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Azas- 1750 Laboratory Rep-- o Revr isiton 1 Analytical Report of Data for PFOS Dietary LC50 Study in Northern Bobwhite (Sera and Liver) Laboratory Report No. FACT-TOX-129 (U2723) Testing Laboratory 3M Envir3onMmeEnntvailrTonemcehnntoallogLyab&orSaatfoertyy Services FiuorineAnalytical2C3h6e0mi9stry Team (FACT) S9t3.P5aBuuls, hMANve5n5u1e0,6 Laboratory Contact KiBsldHga.ns2e3nE,.P0h9D. St.PauPl.,O.MBNox553133331-3331 PFhAonXe:: ((665511))777788.-66107168 Revision Date: March 19, 2001 Page 106f 3 WN oslaslos C0054! 1 Introduction ! V3`ii oTh5sigssD3tuoedwsyenwyamts(deeosWiigcineenddotocndqemuyaaantgiaootfantnidvmaeylydta2se,snepsE spdeertrfiolu.d eorSS ooCtctao0nese ulplfocenataeo e (eP)FsOSS,oeGie Fdt;SCOEo yE,CAeSape#r2n79e5. een IMEvinLiao a ani para PosTo rEphcyiiuiodnofotods wasscapl by oraO taeofa be PEOS cen. Witkin thatdiedbeforethescheduledsacrificedatewerecollectedandanalyzedalso;resultsareinckudedinthis. CCoi mipneroo,kFh15e3saero EcRlSOwDiei B edo 1E055pePedo Pom sma oe 0 . 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GNOKEE 4 Methods - AnalayndtPriepcaraatolry St AnalEyTtSi.c8a4l.1m,etEhoxdstaocftPoitasosinum PerfluororoOtohecr aiornocehensicllCofmpoounndsafrtom D`uSeerutmotfohesAnmaallylsvioslUusmiengofHbPoLbCwhEilesceosaapvraaiyllaMbales,ssStpanedcatrodecnuerzvyeswwietrhethperfeoplalroewdiniemxectehpatnioolnsi:nstead of f0i6o7m30b9o9b,whPiRtOeSsIeao.1F7o1uwramsautsieds3pikreesfwereenecepmraetpearrieadfl.omthepoked bobwhite quiserum receivedon f[rFoAmCTL-iMv-1e1r,forEAxntarlaycstiisonUsofinPgoHtPasLiCu-mElPecreoscprafyMaossSrpecotroomoretOrtcyhweirtFhutonreocfcohlelmsoiwcianflgCeooxcmeptpotuone:ds t"iTmheeeoxtfreaxcttriaoctniwoan.spPeRrOfSorlmted1f71owllaoswuisngedET8S-e8r-e6.n;cehismmaetetrhioald.hadbeenvalidatedbut no spprovedst he ELTivSerf4o6rA0n,aExltyrsaicstUisoinnofgHPoPaLsCsiEulmecPetrufslpuaoryoMeacsiasnSepslefoonmteeo rOtwietrhFthhieofoolclhoewriingcaexlcCeoptmipoan:nndsfrom Duoemtbotohbeswmahllviaemero,uIntsoof rbeocbasweist,e iIvveersavmapllbelsew,esraevnedryricmuirtveedsawnedrtewporeopramrredeisnarmepdlehsahlaidsttobaedof pcrepoarmedfbfrooimretxnhaecbetoibodwnh.iTtheesveercPFoOSmlo b17ai1ewnainsduiacsaeitdedaiirnetfhoeerernneceamslat.eTriawenmtatyi-spltkeswweore UEsTiSn.g5H.1P,LCBalleycsitsosofprPaotyaMsasisusmSPpeescfltorrooomc.tncsulfoateor Other lsrochenicals in SerExacs FUsAiCnTg:HMP:L2C.,EAlneacltyrossipsroafyPMoaasssiSpuencPtroemetcrywfituh herfoolowoinocgreOxtctehpetnriFoune:orsochueilcafsoooLinveraExttracets: "eTxhteraancatiloyns.iAswsadesspeerrbfeodrimnedthfelmleotwhaoadg,EaTl SSe-7s.s0,arwehircephorhtaedbeiernvnaslidoafteidgbouftaoltyappperrogvreadm hofeivmeerof E(TveSt8w.e7i.g0h,tAn.alysisofPotassium Perfuoroecapesuforat o Other hirocbemiclsonLiveExits Using HPLC FltospayMassSpectromery. `ASnaampllyetepisrexteracpteda-fHorPmLaaCs/atEmpSilMeSomMatSnr:ixfwoi-tpahiorinnpgienxitnrgarcetaigoennteabuty amano bydrogen lf: (OTnBceAt)heiannaiponH-TcoBnAroplaleidsenfvoiromnemde,tnhte.aTnahlycanaensrefargeendtosetlectnivoelypolgetosragnaionsolveenrtoc(htehnymcals butyl ther,did,adreconstitutedinmeta forMSanalysis HIPnLHOPELCS,MaSnMalSiquotofexci jected an pas through reversepssliquidchromatographic mocboliulmenp.Bhaasseetdohnantahleyatfefisietayiofntehedfanoalyctheafroatcteesrssiaonmaoruynpthoafseven.thFeocroeuxr arelmatiipnvesstloatnehdeai,rdid saonkauityitoenePxFtrOaSctmead yreohmtsaeti8.n0mhiitsaensal.ysRiestwenetieomntaiecshebdeotnwteheenHaPsLaCndsayrsdtePmFtOoSwsoiluitio1n%and be Foorlglaonwiicncg HoPLCmseppairnacotliudoinun,gESfnlMoSrdMocShpsercoa,vlis.deElecpuiodsapnadys,caeionizaetiaonseochrnainquaelyzuisngdpwiidaesranogerof adnetdecatpiroinmoafrmyofloenc,uclhaarriancseriies onfbeearltel,isyaetleeccltaedip.eTlhyeiisfdronbtetmpoereatmudresb. Mwaitohhlrigehdcanweuereigloyngiedzaessd,, Sduebtseectqeudentollsonscestesmallersecoodary i agentsunique the primary i,whichae 1sFofraegxmaemnptlee,dfnorfPoFtOheS o(nCssFcOh)aasn8a0laysmius,(ocnr4e99psisenlgect0eSdaOs t)henchdar2a3c0taemrs (pFriSmaOr)y.on.ETshcihsoifon {mhiegshetseacvoendthaerysfarmagmcheanrtasctedreistteiccte4d9a9anmd apnribmearsyeodnt,odbitfedirfefenrteentPcRhOiSfcraomcoompeorsictoiomnpsosupnddshat seconofrangmendtataionpratys. Page3af3fwe othaik, Croasy 5 Analysis Te S1 Calibration `AaCmklefi,adieovnecchelckasnarhecwkesr aconmpilianetlifyenaenveerny5l1e0sppisevsafsowiito+1r.3s0%ofbeipe.cted Quuhnaeenrcseiasapcoienosdn ooefftchhoenaoorfcmgtovcsslbpayecsckiweeaddsabsaehsxeecdshoronoscoussfiensgelbsesTI.SQyssu(pciga hoetaecdnh1sn 5)hgofetet noisfbeeodca ment Mv1ad021teutmye8of69d%m)ooflPEsOoSnwapsreeddfi clSbhaave pstaedeaS.oAspty fovansok 52 Extraction and Method Blanks `oAsagmepglsewsi.twAhodccicsrhoabncaalcyh,obfwanokmpse,itlshoisdonbdagnwkvset,sulsirocgoaoerliss,lweeresext(acseadlviat)h ,chvebrecehxocfi IBlnanikswedrye,cl omplweirs ceogemtpllyewasdeste hove eitofdecionfoc ssi aye. 53 Surrogates m"oTneironhredyckonof pgeossrsonersvo)aso(uicse,afneceoegslee.nfSuiorosslyersesp,oSseuwogaeceeapspvoaersl ies 5.4 Matrix Spikes Ohveemratticsoprieesvetreerecloyvseisr,ewd yrtissmaaniS0sp3ik0eswereexppeepcveiionewibbtwwoscevpets.iTh,etAoll 5ca0nlguy mreroaconevOeacrtsyopobitfeesrs0edp8t,r1wd9e9ie.Ttrhee3a5r0ee5dxooafncSbteeepdetxmepmeabcetterd2p,vke19.c99oTwvhietshrshewdpiaiyes3g.rs7eda esrainppSlocss1w8nee%edr fToLr niaBlomarmea.y3a ep Bo AaOe, 95 ehoExc de Bsepciaeussteoewbedbaay sreercaovweeryesofigtsecdcanl90u%somfabesxpkedslveies werepepe. 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