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EHPS 104(61Hayes http://ehpnet 1.niehs.nih.gov/docs/1996/Suppl(6)/hayes.html Mortality among Benzene-exposed Workers in China Richard B. Hayes,l Song Nian Yin? Mustafa Dosemeci,l Gui Lan Li? Sholom Wacholder,l Wong Ho Chow,l Nathaniel Rothman,l Yao Zu Wang? Tan Rong Dai,4 Xin-Jie Chao? Zhong Lian Jiang: Pei-Zheng Ye: Hong Bin Zhao,8 Qing Rui Kou? Wan You Zhang,l0 Juan Fei Meng,'l Jie Sheng Zho,12 Xia Fang Lin,13 Cheng Yu Ding,14 Chin Yang Li,l5 Zhi-Nan Zhang,l6 De Gao Li,16 Lois B. Travis,l William J. Blot,l and Martha S. Linetl Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland; 21nstitute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing, China; Stations of Public Health and Prevention of Infection, 3Shanghai, 4Chengdu, and 5Chonqing,China; Institutes of Labor, Health and Occupational Disease, 6Tianjin, 7Heilongjiang, *Luoyang, 9Shenyan, loSichuan, Jiangxi, 12Nanchang,13Guangzhou,14Jinzhou,China; 15Sectionof Hematopathology,Mayo Clinic, Rochester, Minnesota; l6Division of Hematology, Peking Union Medical Hospital, Chinese Academy of Medical Sciences, Beijing, China e Abstract e Introduction e Methods e Results e Discussion Abstract A large cohort of 74,828 benzene-exposed and 35,805 nonexposed workers employed between 1972 and 1987 in 12 cities in China was followed to determine mortality fiom all causes. Benzene-exposed study subjects were employed in a variety of occupations including coating applications, and rubber, chemical, and shoe production. Mortality was slightly increased among workers with greater cumulative exposure to benzene @trend <0.05),but this excess was largely due to cancer deaths @trend -4.01). Deaths due to lymphatic and hematopoietic malignancies = 0.01) and lung cancer @&end =0.01) increased with increasing cumulative exposure to benzene. Investigationscontinue to relate benzene exposure to specific lymphatic and hematopoietic malignancies and other causes of death. -- Environ Health Perspect 104(S~ppl6)1:349-1352 (1996) Key words: benzene, cancer, lymphatic, hematopoietic, lung cancer, epidemiology This paper was presented at Benzene '95: An International Conference on the Toxicity, Carcinogenesis, and Epidemiology of Benzene held 17-20 June 1995 in Piscataway,New Jersey. Manuscript received 16 January 1996;manuscript accepted 14 June 1996. Address correspondenceto Dr. R.B. Hayes, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6130 Executive Blvd. MSC 7368, Bethesda, MD 20892-7368. Telephone: (301) 496 -9093. Fax: (301) 402-1819. E-mail: havesr@,epndce.nci.nih.gov Abbreviations used: ANLL, acute nonlymphocytic leukemia; HLM, hematopoietic and lymphoproliferativemalignancies; ICD, International Classificationof Diseases; RR, relative risk; SMR, 1 of6 11/7/97 10:32AM EHPS 104(6) Hayes standardized mortality ratio. http://ehpnet1.niehs.nih.gov/docs/l996/Suppl(6)/hayes.html Introduction Benzene is a solvent widely used in industry and found in cigarette smoke, gasoline, automobile emissions, and other products (1). Benzene exposure has been related in numerous occupational studies to increased risk of acute nonlymphocytic leukemia (AWL) (2) and, in some reports, to other lymphohematopoieticmalignancies (3-5). In 1981, the Chinese Academy of Preventive Medicine (CAPM) carried out a national occupational survey in which more than 500,000 workers exposed to benzene in China were identified (6). Subsequently,28,460 of these exposed workers and 28,257 nonexposed workers were followed for cancer mortality between 1972 and 1981 (7,s). Significantly elevated standardized mortality ratios were noted among exposed compared to nonexposed workers for leukemia (standard mortality ratio [SMR] = 5.74) and lung cancer (SMR = 2.3l), whereas elevated but nonsignificant excesses were noted for cancers of the liver, stomach, esophagus, intestine, nasopharynx, and lymphosarcoma (8). Since 1987, the U.S. National Cancer Institute (NCI) has collaborated with the CAPM to expand this study in cohort size, duration, recency of follow-up, and exposure assessment. Previously (9), we described the overall risk for benzene-associated diseases in this cohort, including excess risk for leukemia and lymphoma among benzene-exposed workers. In the current study we utilize information on levels of benzene exposure and report on the exposure-responserelationship with respect to benzene and mortality risk. Methods The methods for this expanded study are described in detail elsewhere (10,ll). The benzene-exposed group was comprised of workers employed between 1972 and 1987 in 1427 selected benzene-exposed work units (departments) in 672 factories in 12 cities in China. Workers in a variety of industries and occupations that use benzene were studied; the occupational areas included painting, printing, and manufacture of footwear, paint, and other chemicals. A nonexposed comparison group was assembled from workers employed between 1972 and 1987 in work units in which benzene was not used in 69 of these factories or in 40 additional factories. Subjects were identified from initial employment, salary, and other factory records. We abstracted demographic data, including birth date, sex, and occupational data, including the dates of employment by work unit and job title for all jobs held by subjects in the study factories. Benzene-exposurejobs were determined from factory-level and job title-specific information on benzene use. For the cohort study, average occupational exposure to benzene (in ranges of <1 ppm, 1-<5 ppm, 5 - 4 0 ppm, 10-<25 ppm, 25-<50 ppm, and 50 + ppm) was estimated by local industrial hygienists and other industrial health personnel for seven calendar periods (1949-1959, 1960-1964,1965-1969,1970-1974, 1975-1979, 1980-1984, 1985 and later) and for study-specificjob titles (11). A total of 18,435job and calendar period-specific exposure estimates were made, 38% based upon monitoring data collected primarily after 1975. When no monitoring data were available or when the measurement results were not consistent with other exposure information (including amount of benzene-containingmaterials, percent of benzene in the materials, average frequency of exposure, and changes in industrial hygiene controls), monitoring data from other calendar periods were used, with adjustment for historical changes and exposure frequency. If no monitoring data for the specificjob were available in any calendar period, monitoring results for similarjobs were used after task descriptions and historical changes were considered. If none of the above sources were available, the field center staff used all available exposure information and their professional judgment to estimate the exposure. Subjects were followed for selected lymphohematopoieticmalignancies and other hematologic disorders and for vital status to 31 December 1987. We used factory personnel records at study factories and subsequent places of employment or, when necessary, contacted next-of-kin, work colleagues, treating 2of6 11/7/9710:32 AM EHPS 104(6) Hayes http://ehpnet1 .niehs.nih.gov/docs/l996/Suppl(6)/hayes.html physicians, or others. For deceased subjects, cause of death was obtained from medical records, other written factory records, or death certificates. Only after extensive search had failed to locate written records listing cause of death were treating physicians or next-of-kin contacted. For any worker suspected of having any hematopoietic or lymphoproliferativemalignancy or nonmalignant related disorder, extensive efforts were made to obtain detailed medical records, hematological laboratory reports, histopathology reports, and pathology specimens. Details of the specific information abstracted and the review of clinicopathologic material by expert U.S. and Chinese hematopatholgists are described elsewhere (12,13). For the statistical analysis, subjects employed less than 6 months and those hired before 1949 were excluded. Person-years were accumulated for the benzene-exposed workers, with a 1.5-yearlag, from 1 January 1972 or, if hired later, from the first date of employment in a benzene-exposed job until death, loss to follow-up, or 3 1 December 1987. For the nonexposed comparison group, person-years were accumulated from 1 January 1972 or, if hired later, from the first date of employment. Analyses of mortality were made by internal comparison of disease rates in the benzene-exposed group to the rates in the unexposed group, with adjustment by Poisson regression for age and sex as appropriate, yielding relative risk (RR) for exposed versus nonexposed workers (14,15). Tests for trend of increasing risk with increasing level of benzene exposure were carried out. Results The study group consisted of 74,828 benzene-exposed and 35,805 nonexposed workers. On average, benzene-exposed subjects were followed for 10.5 years, while nonexposed subjects were followed for 1 1.7 years. Women contributed 47%of the person-years in the benzene-exposed study group and 40% in the nonexposed group. Overall, the study groups were young, with about 60% of the total person-years at risk contributed by subjects less than 30 years old at study entry. In this young study population, about 2%died during the follow-up period (1,369 benzene-exposed and 598 nonexposed). Only 147 exposed (0.20%) and 90 unexposed (0.25%)workers were lost to follow-up. Table 1. Mortalityaamng Chineseworkers. cumulativebenzene apcsure. Causeof death (CD.Sfb Revi?h)B None? <10 Curmlative apcrsure. ppm-years 10-3 404a 1o w 0 0 400+ All causes RR 1.o 1.1 1.o 1.1 1.1 1.2 No. cases Ea 158 133 210 ?el 376 Infcctious and pmsitic (001-ius) RR 1.o 1.2 1.2 0.4 OB 1.4 No. cases D 7 9 3 11 15 Malignant neoplasm (140-206) RR 1.o 1.1 09 1.o 13 1.4 No. cases 218 Ea 63 70 163 155 Circulatorysystem(3W99) RR 1.o OB 1.o 1.1 1.1 t .o No. cases 21 0 28 73 84 147 134 Respiratory system(450-5lg) RR 1.o 4.2 2.2 OB 09 19 No. cases 14 12 11 4 9 18 DigH i v esystem(520-5754 RR 1.o OB 0 3 0.7 09 0.7 Gihasis (571) No. cases 51 9 5 11 25 18 RR 1.o OB 0.4 OB 1.1 09 No. cases 35 6 4 8 22 15 Urinary tact (580-629) RR 1.o 1.2 0.7 OB 0.7 0 9 No. cases 16 6 4 4 66 Occupational injury. poisoning RR 1.o 15 OB 4.7 0.7 5.4 No. cases 4 2 1 5 15 Fonsderej as relative risk(RfQ.adjust& forageand sa.Vromthe World Health Olganization(2q. CReferentcategory: RR = 1.Ofor nonexpcrsaiworkers. P fc As shown in Table 1, all-cause mortality is slightly increased among workers with greater cumulative exposure to benzene @trend <0.05),but this excess is largely due to cancer deaths @wend <0.01). In general, other nonmalignant causes of death were not related to cumulative benzene exposure except for occupational injuries. Deaths due to all hematopoietic and lymphoproliferativemalignancies (HLM) = 0.01) and lung 3 of6 11/7/97 10:32AM EHPS 104(6) Hayes http://ehpnet1 .niehs.nih.gov/docs/1996/Suppl(6)/hayes.html cancer (ptrend = 0.01) were increased among workers with greater cumulative exposure to benzene (Table 2). Other cancer sites showing suggestivebut not statistically significant excesses include nasopharynx and esophagus. Tabk2CancermotlaIkyaamngGhinsseworkers. bycumuhtivebenzene apcsure. Cause of death (ED,9!h Revkrbng Malignantneoplam (140-208) Nasophwynx(l47) ESOPhgUS(15Q S t o m c h (151) Colonand mtum(153,154) Liverand gall bladder(155,156) Tmchea. bmncwand lung(l6Z) Brain and othercentml nervoussystem (191a 7-25) Hematopoietic mlynancies (200-208) RR NO. cases RR No. cases RR No. cases RR No. mses RR No. cases RR NO. cases RR No. cases RR NO. cases RR No. cases Nond 1.o 218 1.o 3 1.o 7 1.o 43 1.o 17 1.o 49 1.o 41 1.o 5 1.o 12 <lo 1.1 99 2.7 2 35 5 08 6 15 7 1.1 12 1.2 10 OB 1 25 9 Cumlative aposure. ppm-ymrs 10-3 4- 1O W 0 09 1.o 1 3 63 70 163 4.4 - 25 4 04 0 5 13 1.1 1 35 1.o 09 1.o 13 12 25 0.7 05 OB 4 38 OB 08 16 12 9 44 1.o 1.4 1.4 13 19 38 1 9 1 3 0.4 3 21 2.1 2 9 3.1 a 10 18 400 + 1.4 155 29 4 3.2 13 1.2 27 1.4 12 1.2 B 17 41 23 5 2.0 9 T;onsklereJ as relative risk(RM,adjustej forageandsex; 'Fromthe World Health Organization(Z.!j) CReferentcategory:RR=l.Ofornonexposd workers. F h Discussion We found increased mortality due to lymphatic and hematopoietic malignancies, lung cancer, and occupational injuries in association with increased exposure to benzene. Suggestive associations were noted for other causes of death, including nasopharyngeal and esophageal cancer. Because of the known relationship of benzene as a risk factor for ANNL and preliminary evidence from this and other studies implicating benzene in the etiology of other HLMs (2-5), we are continuing statistical evaluations of this association In experimental studies (16,17) benzene has been found to cause cancer at multiple sites, suggesting that excesses of hematological and nonhematological tumors in humans could be causally related to exposure. Lung cancer mortality showed an exposure-responsepattern in our study. Benzene exposure has not previously been related to lung cancer (2) except for an earlier report from a subset of the present cohort (8). In that report an overall excess was shown in a comparison of ever- and never-exposed workers (SMR = 2.3), but no evaluation of level of benzene exposure was made. In the current study, however, we show that the excess risk parallels increases in cumulative benzene exposure. Some benzene-exposed workers in this cohort were employed in occupations that have previously been associated with increased lung cancer risk; these workers include painters (18) and leather workers (19). We plan further studies to determine if the association of benzene with lung cancer risk is due specificallyto benzene or to other types of exposure, including tobacco use. Suggestive increases in risk were seen for nasopharyngeal and esophageal cancer. Continued monitoring will provide opportunities to study these associations in more detail as additional tumors are identified in the aging study cohort. Excess mortality was also found due to industrial accidents among workers highly exposed to benzene. A limited number of epidemiologic investigationshave provided the scientific base for determining the quantitative relationship between benzene exposure and cancer risk (20-22). Because we have characterized exposure in detail for a large number of benzene-exposed men and women, data from this cohort should provide a substantive addition to current knowledge. 4of6 11/7/9710:32A M EHPS 104(6) Hayes http://ehpnet 1 .niehs.nih.gov/docs/1996/Suppl(6)/hayes.html The study had several additional strengths. Although the present analysis is limited to mortality outcomes, cases of lymphatic and hematopoietic diseases have been identified and classified by disease type following review of available pathologic material and medical records (12,13). This detailed information will be utilized in hture reports. Quantitative exposure estimates have been developed for cohort study subjects (11) and these estimates correlate with the occurrence of benzene hematotoxicity (23), supporting the validity of our approach to exposure estimation. Complementary to these investigations, biomarker studies among a series of workers with current benzene exposure and among workers with a history of benzene toxicity are being analyzed to provide insight into mechanisms of benzene-associated disease in humans (24). Several limitations should also be noted. Detailed evaluation of more than 110,000 study subjects necessitated a large number of investigators at many sites, requiring extensive efforts at study coordination. Although not unique to this study, exposure assessment relied upon limited measurement data, particularly for the early years of study. Efforts are continuing to refine and validate these estimates. The cohort is young and follow-up continues to characterize further the long-term effects of benzene exposure as the cohort ages. In summary, mortality due to lymphatic and hematopoietic malignancies, lung cancer, and occupational accidents increased in direct relation to cumulative benzene exposure among industrial workers in China. The results are preliminary; further investigations are planned to characterizethe scope of benzene as a human carcinogen in this large cohort of industrial workers. References 1.Wallace LA. Major sources of benzene exposure. Environ Health Perspect 82:165-169 (1989). 2.IARC. Benzene. In: Some Industrial Chemicals and Dyestuffs. IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. Vol29.Ly0n:International Agency for Research on Cancer, 1982;93-148. 3.Goldstein BD. Is exposure to benzene a cause of human multiple myeloma? Ann NY Acad Sci 609:225-334(1990). 4.Young N.Benzene and lymphoma. Am J Ind Med 15:495-498(1989). 5.Mehlman MA. Benzene health effects: unanswered questions still not addressed. Am J Ind Med 20:707-711(1991). 6.Yin S,Li Q, Liu Y, Tian F, Du C, Jin C. Occupational exposure to benzene in China. Br J Ind Med 44:192-195(1987). 7.Yin SN, Li G, Tain F, Fu Z, Jin C, Chen Y, Luo S, Ye P, Zhang J, Wang G, Zhang X, Wu H, Zhong Q. Leukaemia in benzene workers: a retrospective cohort study. Br J Ind Med 44:124-128(1987). 8. Yin SN, Li G, Tain F, Fu Z, Jin C, Chen Y, Luo S, Ye P, Zhang J, Wang G, Zhang X, Wu H, Zhong Q. A retrospective cohort study of leukemia and other cancers in benzene workers. Environ Health Perspect 82:207-213 (1989). 9.Yin SN, Hayes RB,Linet MS, Li GL, Dosemeci M, Travis LB, Li CY, Zhang ZN, Li DG, Chow WH, Wacholder S, Wang YZ, Jiang ZL, Dai TR, Zhang WY, Chao XJ, Ye PZ, Kou QR, Zhang XC, Lin XF, Meng JF, Ding CY, Zho JS, Blot WJ. A cohort study of cancer among benzene-exposed workers in China: overall results. Am J Ind Med 29:227-235(1996). 10.Yin SN, Linet MS, Hayes RB, Li GL, Dosemeci M, Wang YZ, Chow WH, Jiang ZL, Wacholder S, Zhang WU, Dai TR, Chao XJ, Zhang XC, Ye PZ, Kou QR, Meng JF,Zho JS, Lin XF, Ding CY, Kneller R, Blot WJ. Cohort study among workers exposed to benzene in China. 1 : General methods and resources. Am J Ind Med 26:383-400(1994). 11. Dosemeci M, Li G-L, Hayes RB, Yin S-N, Linet M, Chow W-H, Wang Y-Z, Jiang Z-L, Dai T-R, Zhang W-U, Chao X-J, Ye P-Z, Kou Q-R,Fan Y-H, Zhang X-C, Lin X-F, Meng J-F, Zho J-S, Wacholder S, Kneller R, Blot WJ. A cohort study among workers exposed to benzene in China. 2: Exposure assessment. Am J Ind Med 26:401-411(1994). 12.Travis LB, Li CY, Zhi ZN, Li DG, Yin SN, Chow WH, Li GL, Dosemeci M, Blot W, Fraumeni JF JR, Hayes RB, Linet M14S:9. 1H-e1m02at(1op9o94ie)t.ic malignancies and related disorders among benzene-exposed workers in China. Leuk Lymphoma 13.Linet MS, Yin SN, Travis LB, Li CY, Zhang ZN, Li DG, Rothman N, Li GL, Chow WH, Donaldson J, Dosemeci M, Wacholder S, Blot WJ, Hayes RE%.Clinical features of hematopoietic malignancies and related disorders among benzene-exposed workers in China. Environ Health Perspect 104(Supp16):000-000 (1996). 5 of6 11/7/9710:32AM EHPS 104(6) Hayes http://ehpnet 1.niehs.nih.gov/docs/1996/Suppl(6)/hayes.html 14. Breslow NE, Day NE. Statistical Methods in Cancer Research. Vol2: The Design and Analysis of Cohort Studies. IARC Scientific Publications Series 82. Ly0n:InternationalAgency for Research on Cancer, 1987. 15. Preston DL, Lubin JH, Pierce DA, McConney ME. Epicure User's Guide, Seattle, WA:Hirosoft International Corp., 1993. 16. Maltoni C, Ciliberti A, Cotti G, Conti ByBelpoggi F. Benzene, an experimentalmultipotential carcinogen: results of the long-term bioassays performed at the Bologna Institute of Oncology. Environ Health Perspect 82:109-124(1989). 17. NTP.Toxicology and CarcinogenesisStudies of Benzene (CAS No. 71-43-2) in F344/N Rats and B6C3F1 Mice (Gavage Studies). TR-289. Research Triangle Park, NC:National Toxicology Program, 1986;ll-101. 18. IARC. Occupational exposures in paint manufacture and painting. In: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Vol47: Some Organic Solvents, Resin Monomers and Related Compounds,Pigments and Occupational Exposures in Paint Manufacture and Painting. Ly0n:International Agency for Research on Cancer, 1989;329-442. 19. IARC. Leather. In: IARC Monographs on the Evaluation of the CarcinogenicRisk of Chemicals to Humans.Vol25: Wood, Leather and Some Associated Industries. Ly0n:InternationalAgency for Research on Cancer, 1981;201-292. 20. Rinsky RA, Smith AB, Hornung R, Filloon TG, Young RJ, Okun AH, Landrigan PJ. Benzene and leukemia: an epidemiologic risk assessment. N Engl J Med 316:1044-1050 (1987). 21. Ott MG, Townsend JC, Fishbeck WA, Langner RA. Mortality among individuals occupationally exposed to benzene. Arch Environ Health 33:3-10 (1978). 22. Wong 0.An industry wide mortality study of chemical workers occupationallyexposed to benzene. 2: Dose response analyses. Br J Ind Med 44:382-395 (1987). 23. Dosemeci MyYin SN, Linet M, Wacholder S, Rothman N, Li GL, Chow WH, Wang YZ, Jiang ZL, Dai TR, Zhang WU, Chao XJ, Ye PZ, Kou QR, Fan YH, Zhang XC, Lin XF, Meng JF,Zho JS, Blot WJ, Hayes RB. Indirect validation of benzene exposure assessment by association with benzene poisoning. Environ Health Perspect 104(Supp16):000-000 (1996). 24. Rothman N, Smith MT, Hayes RB,Li GL, Irons RD, Dosemeci M, Haas R, Stillman WS, Linet MyXi LQ, Bechtold W, Wiemels J, Campleman S, Zhang LP, Quintana PJE, Titenko-Holland N, Wang YZ, Lu W, Kolchana P, Meyer KB, Yin SN. An epidemiologic study of benzene's early biologic effects in heavily exposed workers in Shanghai, China. Environ Health Perspect 104(Supp16):000-000 (1996). 25. WHO. International Classification of Diseases. 9th Revision. Geneva:World Health Organization, 1977;1-773. __ [Table of Contents] Last Update: Febuary 12, 1997 - -- [EHIS Home1 [Search EHPl [Comment on article1[Tech Assistance] [SubscriptionOptions1 [Single Copy Order Form] 6of6 i 11/7/97 10:32 AM