Document zwdkQ3RmOxd8b4k0EqYEKpV0

AR226-0342 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: N-Ethylperfiuorooctanesulfonamide; may also be referred to as UOc1t4a6n4e,suEltfFoOnSaAm,idEet,P1O,S1,A2,,2F,-36,330,94.,4o,5r.F5X,6-61,27.,7.(81,-8.8heptadecafluoro-N-ethyl-, CAS # 4151-50-2) Remarks: Material is a white solid. Sample purity and lot number were not recorded. METHOD Method: OECD 209 Test type: Static acute GLP: No YAneaalrytCiocmapllmeotneidt:or1i9n9g8: Dissolved oxygen concentrations. Test organism source: Activated sludge collected from the Western Lake `Superior Sanitary district, Duluth, MN Test conditions: Dilution water: Carbon-treated dechlorinated City of Duluth, MN, tap water Synthetic Sewage: 1 liter DI water 16.0g Bacto. peptone 11.0 g Beef extract 3.0g urea 0.7 g NaCl 0.4 g CaCl 2H;0 0.29 MgSO4* 7H,0 2.89 KHPO, Reference and test solution preparation: A 500 mg/L stock solution of the reference substance 3,5-dichlorophenol was prepared using Millipore water. Concentrations tested were 4, 16, and 32 mg/L, prepared by diluting the stock solution with Millpore water. EtFOSA was added directly to test vessels rather than by volumetric addition of a stock solution. Test vessels: Mixtures were prepared (500 mL final volume) and aerated in 1000 mL borosilicate glass beakers and then transferred 006077 into 300 mL Biochemical Oxygen Demand (BOD) bottles to obtain oxygen uptake rates. Number of concentrations: One, in duplicate plus three reference substance concentrations and two Blank controls Temperature: 20.1-21.8C Total Suspended Solidsof sludge on day of testing: 6.5 g/L (TSS in test solutions = 3.3 g/L). Sludge was used one day after collection. Element Basis: Respiration inhibition as determined by dissolved `oxygen concentration. Test mixtures were prepared at 7-minute intervals and aerated. After 30-minutes and 3-hoursofcontact time, dissolved oxygen was measured every 0.5 minutes for up to 5.5 minutes. RESULTS _ _---- Nominal concentrations: Two blank controls, three reference substance concentrations, 1000mgiL (in duplicate) test material solutions Statistical Analyses: ECs values were calculated for the reference material by the Trimmed Spearman-Karber Method. An ECs value could not be calculated for the test substance. Analytical Methodology: Analysis of DO concentrations in all test solutions were performed at AScl Corporation using a YSI Model 54A dissolved oxygen meter. Dissolved oxygen readings were recorded every 30 seconds for 5.5 minutes. Respiration rates were normalized for 20C prior to calculating percent inhibition. Respiration Rates and Percent Inhibitions after 3-Hours Exposure Treatment ResRpairtaetion mg GyLimin. Ipnhoirbciotniton [ES-- C amos Tor-- r0i20eC --| -- | -- [TestsubstanceT000mgL |omer[35 | Control response: satisfactory; difference between controls ranged from 6.8% at 30-minutes to 14.4% after 3-hours of`exposure. 006078 Reference Substance: 3-hr ECso = 18.4 mglL indicating the sludge had normal sensitivity. CONCLUSIONS Tnohmeinteaslt tseusbtsstuabnscteanecxheicbiotnecdenatrnaotiionnhiobfit1or0y0e0ffmegc/t Lonafrteesrp3i-rhatoiuorns aotfa exposure. The ECso respiration inhibition) is therefore greater than 1000 mglL. Submitter: 3M Company, St. Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, DATA QUALITY Reliability: Kiimisch ranking 2. This methodology acceptable at the time, lsatcukdsy,anwahliylteicwaellldactoandfourcted using the determinationof the test substance concentration in the test solutions and sdoeltuebriimiitnyatliimoitnoofftthhee tseastmpsluebsptuarintcye. Testing was also done well above the REFERENCES This study request of was conducted at the 3M Company. AScl Corporation of Duluth, Minnesotaat the OTHER Last changed: 5/18/00 006079 gn .~ EAnSveirloCnomnepnotraaliToens/tAinSgchDDiuvilsuitohn SuAbSmailteSdudy3IMDFCo0.10-.068 STUDYTITLE INHIBITION OF U1464 FOR ACTIVATED SLUDGE RESPIRATION DATASTANDARD = OECD GUIDELINE 209 STUDYCOMPLETED MAY 15, 1998 TESTINGFACILITY EAnSvicrlonCmoerpnotraaltTieosnt/iAnSgclD-iDvuilsuitohn 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 PROJECTIDENTIFICATIONNUMBERS . AScl Study ID# 5010-068 3M Company Lab Requests U1464 SSppoonnssoorr: Su3dMy CIoDmIpUalndyss 1 006089 ot EN ." Study Title {| Data Standard f `Sponsor's Representative f Sponsor'sLabRequest . `Testing Facility (oTPreimnicinpablFiIanvinetsctiBgnart_oree 1.0 GENERAL INFORMATION EAnSvciSlruoCnbomriepnaotracaltoiToe3nsM/tiAnSCgeol.D-iDv-uil1su5ito3hn Ase Sudy 104 3010.068 Inhibition of U1464 For Activated Sludge Respiration. l OECD Guideline 209... 3M Environmental 2-3E-09, 935 Bush Technology Avenue, St. and Safety Paul, MN Services,Building 55133; Tel No. | | (612) 778-7452. lowe. SRaofcehteyllSeerRv.icReos,biBdueialud,in3gM2-E3nEv-i0r9o,nm9e3n5taBlusThecAhvneonluoeg,ySat.nd | Paul, MN 55133; Tel No. (612) 778-7065. i AScl Corporation/AScl-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. [Doepeianrews | No. (218) 722-4040. l Data Validation Review and Report Alan Mozol Report Sig 0 | NW We | Retention of Raw Data and Final| Five years from the date study LF is completed. | | Report | Location of Raw Data and Final | AScl Corporation/AScl-Duluth EnvironmentalTesting I Report -| Division, 4444 Airpark Boulevard, Duluth, 1 MN 55811; Tel. No. (218) 722-4040. ra ore Sponsor: 3M Comy 2 006081 ve D EAnSveirloCnmoernptoarlaTiens/tiAnegl-DiDvuilsiuohn Lo. `Su'AbSmeilneSstutoy3IMDFCo5.01-0505688 2.0 OBJECTIVE |[TUo14d6e4tefromrinaec,tiivfaptoesdsisbllued,gethreesp3i0r-amtiionnu.te and 3-h (contact times) ECyy's of the toot substance |l |i | Ghuiisdesltiundey2w0a9s(cOoEndCuDct1e9d93a)c.cording to the AScI study _ plan retained _ by the Sponsor and OECD 3.0 TEST METHODS Test Substance | Reference Substance Properties: U1464, white crystals, specific gravity = 1.7 (water = | 1), stable, and negligible solubility in water. I Test concentrations: 0 and 1000 mg/L. prepared by direct addition. | 0N9a6m1e5:KG3.,5-dichlorophenol, purity 97%, Aldrich Chemicals, Lot# | | | `Test Organisms Test concentrations: 0, 4, 16, and 32 mg/L prepared by direct | addition of 500 mg/L stock solution in Millipore water. The reference test was performed concurrently with the exposure for | U1464. Results and control chart appended to report. i SDiosutrrciec:t DAucltuitvha,teMdinsnleusdogteafrcoolmletchteedWeosnteAprrnilLa3k0e, S1u9p9e8r.ior Sanitary | i Atocttailvastuesdpesnldueddgesoclhiadrsacctoenricsetnitcrsa:tioTnheofs6l.u5dgge/Lwaats dteesttertimmien.edTthoehatovteala suspended solids concentration in the test solutions was 3.3 g/L. The |1 | imniixttiautrieon.wasThfeedsslyundtgheetwiacssuesweadgeonfeeeddaaynadftaeerracotleldecattio2n1.C until test | Synthetic Sewage Feed | Composition: {I | `SSppoonnssoorr: St5uMdy CIoDm#pUanndysd Bacto. peptone. UBreeeaf extract CNaaCCll, 2H,0 MKg,SHOP,O.,7H,0 16.0 31.10.0 00..74 02.82 3 || 006082 weo a Dilution Water Test Setup | DReestpeirrmaitniaotnion of | { | catcutation of | Respiration Rate { Endpoint Calculation ESnvei`lrSouCnbmmoeirnnpetdaolrtaoToe3niM/nAgGSoc.Dilv--i9Dsi5uo8an "ASe Sudy 1D 3010068 S`Tohuercmeo:stCraercbeonnt-tcrheeamtiecdaldeacnhalloyrsiinsatoefdpCoisttycoafrbDounlu(tPhC,)MwNat,ertaips iwnattehre. | Raw Data Package. Ecoxnptorsoulrseanvedss1eltse:st 4c,on1c-enLtbroartoisoinliecxaptoesgulraessinbedaukpelrisc;atet.wo inoculum | Orderof preparation: At 7-minute time intervals: (1) first inoculum | control, (2) test exposure concentration replicates, and (3) second inoculum control. The final volume in each beaker was 500 ml. Pardedpeadra1t6iomnl: o(f1s)yAntdhdeetidcrseeqwuairgeedfaemedoutnoteoacfhcabrebaokne-rt,reaantded(3w)atienr,tes(t2) | exposures added the required amounts of the test substance, (4) and | added 200 ml of the sludge, for a final test solution volume of S00 { ml. # Incubation and aeration: 20.1 to 21.8C and at an aeration rate of | 0.5 to 1.0 liters per minute. Sequence: Same as exposures were prepared. f Determination: At 30-min and 3-h contact times: (1) filled one 300- ml BOD bottle with the exposure medium and continued to aerate the remaining stirring pr o medium, (2) using be, determined the a YS DO I S4A oxygen concentration meter in the witha self- exposure | `medium every 0.5 minutes for up to 5.5 minutes (the first | determination was made was placed back into the after 1-L 0.5 minutes), and exposure chamber. (3) the sub-sample DO meter I ceaalcihbrcaotnitornolchoercktsrewatemreentm,ade between the sets of measurements for | For each exposure, calculated the slope correlated DO depletion of the regression line as and the time interval, and respiration rate. | ECsy: Trimmed Spearman-Karber Analysis (Hamilton, 1977). SSppoonnssoorr:Su3dMy CIoDnFyU1ch 4 006083 WI , EA5rtrConpoorTiooton/DDAilSane . SoRvmSineSdatyo M04Co3.01030658 3.0 RESULTS [Test substance Toxicity 30-minue ECgy: > 1000 mg/L. 1 (Pertinent data are in Tablel1)shecg >ioomgr. ] |l 4.0 TEST QA/QC QAQC Criteria Respiration rates of two inoculum controls must not vary by more than 15%. 3-h ECs of reference substance must be between 5-30 mg/L. `Test duration must be 3 h. [~ own ~] | Variations after 30-minute and 3-h contact times were 7% and 14%, respectively. | 3h ECgg was 18.4 mg/L, indicating the sludge `had normal sensitivity. | The test duration was 3 h. J --_--_-- 5.0 REFERENCES Hamilton, M.A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in `Toxicity Bioassays. Environ. Sci. Technol. 11(7): [ 714-719; Correction 12(4):417. 1978. { TOersgtainnigzaotfioCnhefmoircEalcso.noOmEicCDCooPpuebrliactaitoinonanIdnfDoermvaetlioopnmeCenntte(rO,EWCaDs)h.in1g9i9o3n.,ODE.CC.D Guidelines for SSppoonnssoorr: Su3dMy CIofmpDaindyss s 006084 CTEACLaDCUnALCATT10IIVOANTOEFDRSELSUUDLTGSE.RESPIRATION INHIBTION TEST 209 Test Subatance: 1464 Any: OIERKES 30Mote Exposure Otsoived Oxygen Concenations, man. EmoitCopTomniDinesss a aiyo on oo3 o PfoH rseeere]] eeF l17805 r 751[775217659[[7e16[6635r 6|6s0[[6523]55:35556012211r 22[ToosreewsJJoosssses]l 4 5] ] H er sT e e a r err rr r Tr rTr Tr -- r r r r GazTecso]nvB:alv6s8|76[75]G57006|55% 6Gi.|6130010m6a0l157 [sa|208 |GwsosFfaoc GsF s05a37]8 3 ou Exposure Dissolved Osygen Concomraions, mgt f[Peeossooassl] os0r0ef]l8867.61.T8ss8sl1l8s5s3]|Tse8aa1ll[7o89o1]a770.z]822..7a1l77203.001.v2261]]177604] 2z0o0..s1 o00..sa4013e068oJ[aondooeasrTl|] 3460|]] E E FrrrE a r T r ee . [r T= aceroe4a:l Tshaalsal o3Ce ]5703(3]5%vCai:l373T 0050mavlaT l val a mslo--se--slFa1mo--5:w10s7--97]0-- SQOahveAcs mcmsceamnnttio St10 ntsecc,eRmniAshRsC om rtmtb 3 @ i Spnoonr.s3t4yCoomrpaonye c[hae ssromc or se o ffeeraseeen