Document zvYNb3bjvp24QNrR6V8vX7Q6

Cpe | | | | | | | 4 | |. | | | | ARYA O09 SUMMARY OF HISTOPATHOLOGICAL EXAMINATIONS OF FATHEAD MINVOW (Pinephales promelas) EXPOSED TO 78.02 FOR 30 DAYS. Fe-9s RESEARCH REPORT SUBMITTED 70 3M COMPANY ST. PAUL, MINNESOTA ~ sarone mcre-11e352 Dan ns ci G,Blonomics en Brecet Y Wareham, Massachusetts . ' 001505 | I INTRODUCTION | | | |. An "egg and fry test" was conducted between June 3 and July 6, 1978 (Bionomics Report BW-78-8-263, August, 1978) to assess the sub-tethal effects of 14c-79.02 on fathead minnows (pimephales promelas). Effects on egg hatchability and on survival and growth of fry were measured. To further investigate possible effects due to exposure to 14C-78.02, histopathological examinations of exposed fish were also performed. mhe results of these examinations are reported here. | MATERIALS AND METHODS A Upon termination of the egg and fry study, five fish from | each replicate of the high 14C-78.02 concentration (1.9 mg/t, measured) were preserved in 108 buffered formalin and sent to the Environmental Pathology Laboratories, Inc., Carolina, Rhode | Island. Control fish vers also examined. | (The fish were examined for gross lesions, and sagital sections were prepared by a pathologist. Fish were placed with identi- . fying numbers into processing cassetts, dehydrated, cleared | ana infiltrated on an Auto-technicon tissue processor using ! the method of the Armed Forces Institute of Pathology. The i fish were then placed in a vacuum oven and subsequently embedded | in parrafin. All blocks were sectioned at 6 microns: one | 001506 [ 2 | slide was prepared from each block and stained with hematoxylin | and eosin. Special staining procedures such as PAS, Trichrome and Acid Fast were used occasionally when requested by the | pathologist. ALL blocks vere sialea with parattin and stored. Slides were labeled, boxed and delivered with all work sheets | to the pathologist for examination. Two slides were prepared from each fish. Tissues processed and examined included but | were not limited to; gill, thymus, liver, spleen, heart, gonad, kidney, foregut; hindgut, olfactory mucosa (mares), brain, ear, skin, muscle, pancreas and pharyngeal mucosa. ~ RESULTS - A summary of the examination of each fish is reported in Table 1. Only those tissues which were missing or contained demonstrable | change are listed: | \The only tissue changes observed in fish in the study vere fatty i change of the liver of fish from both groups and autolysis of : 1 several parts of the digestive tract of fish from the control. i The condition of the livers was consistent with that seen in healthy fathead minnows. . - | In several fish it was obvious that only the deep portion of the | 001507 | 3 affected organs were autolysed while the more superficial | . areas were well preserved. This autolysis appears to be the result of poor generation of fixative. | Based on these data, it is concluded that 30 days exposure to a measured 14C-78.02 concentration of 1.9 mg/t did not | cause any significant, demonstrable, tissue changes in 30 day ola fathead minnow fry. | | 001508 | ' SUBMITTED BY: | | me | Io ! apesoveo | EG & G, Bionomics Aquatic Toxicology Laboratory . December, 1978 stepnen 3. 118 5 Ciro coarse a. cary Director, Aquatic Biology 001509 | Table 1 -- Histopathological examination of fathead minnow (pimephales promelas) exposed 30 days to 1.9 mg/t [ measured 78.02 and control water. | | Fish # Sex Organ Condition Missing Tissue | 1.9 mg/1 78.02 . | 1 male fatty change in liverII none | 2s mfaolmete ffaatcteys cchhaannggee iinn lliivveerr I1I1 neaorn,e chyms | 4 unknown fatty change in liver II gonad 5 female normal car | 6 male fatty change in liver II nares - 7 male fatty change in liver II spleen ! 8 male fatty change in liver IT none | 9 unknown normal gonad I es 10 female fatty change in liver I thymus, spleen | 1 unknown fatty change in liver II gonad | :. 1 . t autoh lysia s inn dpnhairgyvnex,rszr sptaonmcarceha,s intestine, and ; 3 unknown afuattotlyyscihsangien pihnarlyinvxr II gonad, nares : 4 female fauattotlyyscihsangien iinntelsitvierneII spleen 001510 | | Table 1 (continued) | Fish sex Organ Condition Missing Tissue [I | 5 I+ | 7 | | 8 . | 9 | ! 10 unknown ww female unknown unknown . unknown gEmRkEm: faautttoylyscihsangien pihnarlyinvxe,r II intestine, and pancreas satuotmoalcyhs,isiannd pihnatreysntxi,ne afuattotylyscihsangien pihnarlyinvxe,r II stomach, and intestine afuattotlyyscihsangien pihnarlyinvxe,r I stomach, and intestine fauattotlyyscihsangien pihnarlyinvxe,r II pstaonmcarceha,s intestine, and asuttomoalcyhs,isanind pihnatreysntxi,ne ngaorneasd, spleen nares asnpdleegno,nandares nares, gonad spleen, gonad | aLesions graded as follows: " II1 == nmiondienraalte | IITIv == msaervkeerde | I 001511