Document zow91nZYK3b933weDw5yLa98R

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic acid; may also be referred to as PFOA, FC26, or FX-1001. (Octanoic acid, pentadecafluoro-, CAS # 33567-1) Remarks: 3M production lot number 269. The test sample is FC-26. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 - 100% test substance and 0 - 3.5% Ce, C7, and C9perfluoro homologue compounds. METHOD: Method: Not noted. Type: Static acute GLP: No Year completed: 1974 Species: Pimephales promelas Supplier: Not noted. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Graphed LC50 Test fish age: Not noted. Length and weight: Average length = 2 inches Average weight = 1.5 g Pretreatment: Not noted. Test conditions: Dilution water: Not noted. Dilution water chemistry: Not noted. Stock and test solution preparation: Not noted Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Not noted. Number of replicates: one Number offish per replicate: Not noted. Number of concentrations: five plus a blank control Water chemistry during the study: Dissolved oxygen range (24 - 96 hours): 4.7 - 5.7 mg/L (control exposure) 3.8 - 5.2 mg/L (375 mg/L exposure)* pH range (24 - 96 hours): 7.0 - 7.2 (control exposure) 6.0 - 6.7 (375 mg/L exposure)* G03738 * 375 mg/L test group (second highest concentration) data given because total mortality occurred in the 500 mg/L test group (highest concentration tested) by 48 hours. Test temperature (24 - 96 hours): 69 to 70F RESULTS Nominal concentrations: Bk control, 50, 125, 250, 375, and 500 mg/L Element value: 96-hour LC50 = 440 mg/L (C.l. not calculated) Element value based on nominal concentrations CONCLUSIONS The test sample 96-hour LCsofor fathead minnow was determined to be 440 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. This study lacks documentation and information on the methodology. Sample purity was not properly characterized. Test concentrations were not characterized. Testing should have been done in replicate. Health of the fish is not known. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request Number 2485, completed on November 10,1974. OTHER Last changed: 5/24/00 003739 740101 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET LR 2 i P'S TYP* T est , SVftV\C, T est O rgm ie FaVV\Cttfll M ivm flU ? Date S tarted ^4 Time S ta rte d _________ IflOpM D ilu tio n Water M aterial Tested F c - \<ft a f c f l Avg. Weight Date Completed S ite XY\cW<> ^ / / " /<f~74______ P lo tte d so----------^ 4 n s m i / / --------------- BEST COPY AVAll&LE Gone. 24 h rs % D.O S u rv iv al pH wm 48 h rs 72 h rs 96 h rs % D.O % D.O % D.O S u rv iv al pH ppm S u rv iv al pH ppm S u rv iv al pH ppm C ontrol IflO . 7 ? 7 loo 7 * 5 .2 'nOWfl/4 Iaa 7.o T _ Joo. (?7 5 .0 loo 7 o 5 .0 too 6.5 ssn. *>15 loo 6 $ 00 .6 ,3 A?. HO . 6*| * \ k M . . 6.0 as Sqo__ . Dh_. *54 a 5fl 4 ,5 Averase Temperature i 2 _ _______ a ______ 1 > 5.0 100 7<> 6/1 4 p t o 6.6 100 6.4 <V.o 0-- . 3 _______ m _ 72 47 loo 4,5 A M . . 7 4*6 7 4.0 1 61 3.8 0 70 003740