Document zoeDNp0aDdOznkX5LXLDO3VVg
TRADE SECRET
AR226-3191
DuPont-8838
Study Title H-24944: Static, Acute, 96-Hour Screening Test to Pimephales
promelas
Laboratory Project ID: DuPont-8838
AUTHOR: Laurie D. Bouchelle
STUDY COMPLETED ON: November 21,2001
PERFORMING LABORATORY:
E.I. du Font de Nemours and Company Haskell Laboratory for Health and Environmental Sciences
EUcton Road, P.O. Box 50 Newark, Delaware 19714-0050
WORK REQUEST NUMBER: JH|B^ STUDY CODE NUMBER: iHlV
-1-
Company Sanitized. Does not contain TSCA CBI
H-24944: Static, Acute, 96-Hour Screening Test to Pimephales promelas
DuPont-8838
CERTIFICATION
We, the undersigned, declare that this report provides an accurate evaluation of data obtained
from this study.
Work Completed by: ^(lHl[tS^^^Wjk jiQQjL________
Laurie D. Bouchelle
Associate Scientist
^J^tontfeeO Sl06{
Date
Issued by Study Director:
Alan Samel, M.S. Research Ecotoxicologist
Z/ /b.^-^l<^ ^31
Date
Csn-.ps^y Sanitissd. SGSS ns; cGnta'n TSCA CB?
H-24944: Static, Acute, 96-Hour Screening Test to Pimephales promelas
STUDY INFORMATION
Svnonyms/Codesi
DuPont-8838
HaskeU Number: 24944
Composib^^^iff_| Known Impurities:^^^^^B_
C^^^"
Physical Characteristics: Light brown liquid
Sponsor:
E. I. du Pont de Nemours and Company Wilmington, Delaware 19898
U.S.A.
m-Life Imtiated/Completed: October 29, 2001 / November 2, 2001
-3-
Company Sanitized. Does not contain TSC& CBI
SUMMARY
The acute toxicity ofH-24944 to the fathead minnow, Pimephales promelas was determined in
an unaerated, 96-hour, stadc test.
The study was conducted with 4 concentrations ofH-24944 and a dilution water control at a
mean temperature of21.7C (range of 21.6-21.7C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and the 1,10 and 100 mg/L test concentrations were clear and colorless at test start. The 1000 mg/L test concentration was clear and pale tan in color at test start. All water quality
parameters were within acceptable limits during the exposure.
Exposure ofH-24944 to the water control and nominal H-24944 concentrations of
1,10,100, and 1000 mg/L resulted in 0,0, 0,0, and 0% mortality, respectively, at the end of 96 hours. No mortality or sublethal effects were seen in the water control test organisms. The
highest nominal concentration causing no mortality at test end was 1000 mg/L.
The results are summarized as follows:
Nominal concentrations ofH-24944, mg/L 96-hour LCso for H-24944. based on nominal concentrations, mg/L
dilution water control, 1, 10,100, and 1000 > 1000
H-24944 exhibited low concern for aquatic hazard3 in an unaerated, 96-hour, static, acute test using the fathead minnow, Pimephales promelas.
Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASJMSTP 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials,
Philadelphia-
Company Sanitized. Does not contain TSCA CBI
H-24944: Static, Acute, 96-Hour Screening Test to Pimephales promelas
DuPont-8838
TABLE 1
DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES
Nominal H-24944
Concentration (mg/L)
Test Initiation (0 hours)
Dissolved
Oxygen
(mg/L)
PH
Test Completion (96 hours)
Dissolved
Oxygen
(mg/L)
PH
Water Control
8.8
7.7
7.9
7.7
1.0
8.8
7.6
7.9
7.7
10
8.7
7.6
7.6
7.6
100
8.7
7.5
7.9
7.7
1000
8.7
7.4
7.5
7.6
TABLE 2
MORTALITY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24944
Nominal H-24944 Concentration (mg/L)
Water Control 1.0 10 100
1000
Number of Dead Test Organisms /
Number of Test Organisms at Study Start
24 Hours
48 Hours
72 Hours
96 Hours
0/10 0/10 0/10 0/10 0/10
0/10 0/10 0/10 0/10 0/10
0/10 0/10 0/10 0/10 0/10
0/10 0/10 0/10 0/10 0/10
-5-
romnanv Sanitized. Does not contain TSCA CBl