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AR 236 - 0830 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) IEstsusstANCE 000000000 Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1122,334,4556,6,7,7,88,8- heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. Thetest sample is FC-600 (Belgium). Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1%N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 %tolyltriazole. The following summary applies to a mixture with incompletely rcehfalreacctttehreizteodxiccoitnyceonfttrhaetifolnuoorfoicmhpeumriictaielsc.omDaptoane`mnatyonfotthaectceusrtately sample. METHOD: 000000000000 TMeestthotdy:pe:NAotcuntoetesdt.atic (Range-finding) GLP: No YSepeacriCeos:mpPliemteepdh:al1e9s8p3romelas Supplier: Commercial supplier in Brady, Nebraska Analytical monitoring: Temperature, pH, DO Exposure period: 96-hours Statistical methods: Not applicable. No mortality at highest Tceonsctenfitsrhataigoen:teJsutveedn.ile Average Length and weight: Length = 3.6 cm - Weight = 0.39 grams Loading: 0.65 g/L. Pretreatment: Not noted. Test conditions: DDiilluuttiioonn wwaatteerr:cChaermbiosnt-friyl:tered well water, St. Paul, MN. PH: 82 Dissolved Oxygen: 9.6 mg/L Temperaturerange: Lighting: Not noted 18C 002036 dSitroecctkwaenigdhttesstadsdoitliuotnis.on preparation: Test solutions were created by CStoanbcielinttyraoftitohnestedsotscihnegmriactae:l Once solution: Not noted Exposure vessels: of test solution. Wide-mouth one gallon glass jars containing 3 liters Number Number of of replicates: One fish per replicate: 5 WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e Four plus study: two blank controls pH range (0 - 96 hours): 7.9-8.2 7.7 - 8.2 (control exposures) (1,000 mg/L exposure) Dissol5ve.d89O.xy6gmegn/Lra(ncognetr(o0l --ex9p6oshuoruerss)).: Temper1a.t5u9re.6ramngg/eL((01~,09060 hmogu/rLs)e:xposure) 18-21C Resuts 0000 Nominal Element vcaolnucee:ntrat9i6o-nhso:ur2LbClsan=k controls, 1, 10, >1,000 mg/L 100, and 1,000 mg/L Element value based on nominal concentrations. RSuebmsatraknsc:eTReesmtianrgkwsafsielcdo. nTduhcetveadluoen rtehpeomritxetduarpeplaisesdetsoctrhiabtedmiixnttuhree Taensdt not the fluorochemical proportion alone. co0 ncl0 usi0 ons0000000000 T>h1,e0F00C-m6g0/0L.96-hour LCso for Pimephales promelas was determined to be SPauublm,itMtienrn:es3otMa,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St. p0 ataQ0 uaL0 Y 0000000 tReesltiianbgi.litHyo:weKvieirm,istchhersaankmipnlge2p.urTithyewsatsudnyotmeperotpseralllytchhearcraictteeriraizfeord qaunaldi=ty _ tprhoepsotrutidoynliancktsheansaollyuttiicoanls.confirmation of the amount of fluorochemical 002037 This study was conducted by 3M Company, Paul, MN, Lab Request number 8875, 1983. Environmental Laboratory, St. Last changed: 6/28/00 002038 } ` BEST COPY AVAILABLE EolUoKaETTNVoIIn.RyTOOMXGEICNITTAYaL DLaRABTOARrATSOuREoaYErT) w_BA%s ve reAn er a Sgaal nesec, eras tonese22s4g 0 ueion Test OransmA LEi A)ne)sToEAu,r NEcve ateRec'd/JL/B2patch No./-A5 avewe. 2,59 grams Avg. S126O)N No. Organisms par dose 5 Test Solution Volume3Liters/All-Glass Tank Tank Dimensions:WIDE-HOUTH | GALLON GLASS stn Time Started4/0/)#( Date Started 24, R= Date Completed Vz)[A= Dilution Matefilrtere_dweC llwa atet r(Tbew.o/B3nc-., 0.0.7po0n, mG2)) FInavretstsigtaitgH ormas ER= 1g, (95% Contlinms i Lfiimintis))_21000ug/l xfJogo/TiE'T [||madondwed] lTvoet.alboss Foes | on | 0po.u]0, TNootbaerns Foes |pn 0|p|.0 mTootraoe|nbWsoiwsp.Picao. l[fheottai]hHeso fupelo. rfins regalo ules 2 Canteel[I----10 "jl, 9Ia Al0 bas kdl ob 0 csp =IL = [io T= T= 0 BdBol Alo Tana 0Tallzzll0 leila 0 Biol oe s hd ad 9 j=Loo Aloo T=oo ll"7 (=mo|lo dpe Raid 0 Basil 0 1180.5 0 Tahal o his 8 0 Giallo wns rr Tr Lr TI Ter TIT] TostTemp. \ wee I[2 13z7z8 ec/ 1rs26 >0feZ%eBARs5 IiIor2on04o5 lt]iss2/2 %Ji. Come4ntWIsDIITY k LOFGTS, rr---------------------------------------------- Effects other than Mortality oo Trem [ =nNo. A5eastes 6b. ewerallA e |Ape pare [Mp || Aa No. Symptoms or Reactions 24-Hr, 48 Hr, Hr, . Cosolllt "Moe [fue [Awe|| Augie | 2 Ne [| Ape | dee || Ace | 22 pae T Lge | Age [| Jace | Za Wwe [| Loe [Aone || Aaa A Smee Aue 5 5Ta 002039