Document zoL2oJoY34520XZJO7ZMM2xR0

JV ( ` T V - ` ,i J Gi i i;:d i it W j: V !. ` i j'j v 1 - , I** j,,\3 1.1.2;:;': :v;7, (-rim.ro; by Dr P. G. "'lias (Consultant,' , P not a . ; ASI 00004260 Probably bt.-tm m 71-7-.^ of inhaled irila.r.e in ir-pm1' JAtrjC-`- In van moip; abaciption occurs within the first :. than Gccram.ea to an croiilbxin:.: V; twain air/bi nod con- . c.'.m ocnuv bainto, m skin and iron the ga si re-in ho a; (von ircblknycn.., 1953). absorbed through the ibrutes of c,.p'V.u''C! ar.l tiers. ib-.ii-r-nbe abm,': l mecm. ubrer ingschbc: In Due rat, rabbit and dog absorbed trilonc is 'hast Vuiod ar.cn." ell organs and tisruen tut concentrates r.-ootly in fat and brain and I - in skeletal lung and live;, also retain lo'..T levels (bsrreht et, al. , .1939; - : ybon C: Parkhcnsc, 19'o2> von Gottingen, 1333). Gimilur organ concentrations '. or; -maud in guinonplgs tut r.ig levels were also found in ovaries and adrenals (Fabre i ', eh..wt, 1952). In the rat trilcne and trichloracetic acid my be selectively bonne' wo erythrocytes lienee given high, spleen levels (Fabre & Trvlaut, 1952) but plasm pi' beins my also be involved (Soucrk h Vlachova, I960). In ran trilcne is detectable in the blood within 30 none of inhalation (Stewart et &1,, 1962). Trilersc is metabolised slowly to chlomlhyjrate (vj..' or. cnoidde) and than ranid to 2; 2,.2-triu'ilerauetic acid <GC1 COCll) and 2,2,2 triob'i. u otherol (CGI bld^Cd), wrier, latt c,r two suits halites are exciv t'id r.s urinary flu curone.. .0 (e, r, triced 11 ethanol gluey ciciurcnic acid) very little unchanged trilcne acp:vr ' ng in the urine (i cwcll,19 Butler, 1949; Bhl 1 brag, 1953; 'fillinns, 1939; Smith, l` d6). Dogs erc.rcro 5-c`;j of absorbed trilcne as trichloracetic acid and li-GOb r.s ta'.-'hloretkmol up to /, dr's after c:.p:n: ere (Barrett at al., 1939; nameU 6 Johnston. 1930: vor. ueitiv.grn, 1355) The lung ard sole.on, less so the liver are probably the. i r'm altos of metabolism Ilia b?,.c cf iiitc CccjMant not canstitufa fcrr.nl prl iraitcn. il rloulj nc'i La rc x'k'WveJ, ct:s!r:.0 ci C-'-'t .. i v.id'mi tiict F.pn.of tic V7a:'': ti* .`.1 v ' ..'n ;`.<i her; p!cn>> s.ra M,,- <o' . 3 ...wci-r.'-U In ,!, a.-_d Cc flocuciant a cr-itUiie O'lB uno puMicaticn. II r.r d-i! f..iic 1 :'r..t r't'.uc.n co;np'..j mriu ou fCi-vi!"* r; <i';.i o-iiatian tano I'; 'nerii-.'.lien do I O'^ a '.w na . V mi flv !'i Uc-iiL j. o;;t,iv'is CNgi' J ol..-. 1 .1 '.'L.i.103 i.i its n'i;iu:'.(--n'. 1 :> (: 1. '-.Ipuc or i ti\:- a.; :. i. . " ' L - ::n'V;- 1 " nf C '} j v: v.:. ' i" !. ^ -I", 19'b). V f-' .t . - tnuc. . C Ac -I V: GO ' , it of c: i, i' , ,s i '! I. s *..:G 1` . ]1 j i. or-r- v . fc:. ' by .ol- i_ . far up to Is ,:s aft; s . eg. .0 I'- ':c,r ml e aos.-xo she: CO.' ab;'1'". aa .. 'o a... oral by r., .PUCOSC- ? <- . Or .'V1:U f' " of tricMa.v cc Gic ac.'b An ;. , / ; : \z c .. 1 (V Ki.ro ; vrUip oftc-v jr! 1 ;; > I abb.it a r -: -' - 0, 3 ' o; V':, > ob ) v.v cam. o.i nr.444;a, blex-d, l). Cjj .'-'.dr/., ahfjiv. r V - .... i,-. v J . ^ 't Xa.dr: si,, I-}-/'.). Calves a.'.. . A : : rib ' a.rfnici .-raa trilr go triehiorno,;tic acid (if) and trice Vae/harel (13-15*') epp:r4-g in thv.:.. urine bar with a twee of hrb; v. The b'.larva probably . via! or excreted in tin frecvs (Go Go 4 Gchulise, 1955). Hen ever r es 6i"--l6f ol inholoi trilen" as trichloracetic acid (Ahlvarb and Forsevin, IvM); oti:er:.' 'ovivd 75-171 ot retained ty-Mcn.a bain;; e;:cretod as tri.ctl1 or- acetic acid (bv-all, 19/5: Coueeh -./o a!),, 195'*') -an '..'.-ll as t -ic'iloroL'-.ar.nl, nonochloracetic: acid :.' d cbiorcC: '(ivea: ot al.19"1; :7c:."al,'"ie, 1165 ). Cvnll amounts of tricllorscotic acid may cOill:^ to b? e::cr'l.c : in the rri' a ter up to 12 c:yo after inyle exr:osavr (vc;i Oet'/.njen, 1955). 5 human anbiects e::poc-~d for 5 houar to trilene e::c'--et.-:d 4> of tno rc;toi..ad done an r.:-o.:aoc!:lorac-.-tic ac'c, 19t as trichlor acetic acid rr.d 5'1'- ; s t;f ei-lcrothvrol c;v-.v thf- re::t 17. days (douce! 1 vlaciiova, Ive'J; Def'iyje, 19-1). In rViOth'jr enperi-or.t 6 sub;oats inhaled trilmc for 5 bourn. 51-641 of the i.uh:5.;.d t: _'' auo van retained the rapt i,y e>di;l! ed uuchajipod. Of Mo retained triione pd-pOl ran cncrctod as urinary triohl'vcbr.uol ar:i 17-365 as u>l''iry trich?.oracctio acid. C.4`'' cf trichi oiacetic acid and trichlor'et'irjyol aas ease t: a in the faeces, Svoni and saliva contained also both neintoli Mr (bartonice:', Uhl). In all species most of the trichloracetic and trichlorsth-u.ol is cmere tod in the first 2 days after empesure b. t excretion may f~o on up Go 53 days, borne 2-4 hours elapse after single exposure before trichlor,acetic acid ap mars in the blood ranching a maximum in 20-50 hours (Ablmarh 4 Fcrss.man, 1951; Oefalges, 19ol). Trichloretlianol appears to be the main metabolite and is much more toxic (bartouicei: 4 Teisirper, 1962). Jisulfirmn de creases the excretion of trichloracetic acid and trichlorcthar.ol by acting either on converting enzymes or on trilens release from fat depots (Bartonicek 4 Teisinger, 1962) while glucose and insulin increase production (Boucek 4 Vlochova, I960). Chronic exposure may cause disturbance of protein metabolism by an increase in the p-globulir. to 165--216 (normal 5 0;t-l/,6) and in fat v.etobolicm by an increase jn unsaturated fatty acids (Guyot-Jeannir. 4 Van otconkisle, 1956). Bepeatei inhalation or oral ingestion by rats causes transitory elevation of SCOT levels for 24 hours after the last errposure, the SG?T levels remaining normal. 6G0T levels return to normal within 9 days after exposure, bo such transitory effects are seen in rabbits (folor et al., I'/Ao; Via!liar 4 Casanova, 1965). Previous ingestion of ethanol potent lutes ir.lleue toxicity in rots as shown by a rice an fdrT and biOij (isocitric dehyirogcr/ase) and i.-ide-nporoad deyei'erauive lipid infiltration s,s '.:ell as early cenlrilobular necrosis of the liver (Cornish 4 udefuin, 1966). Single e'vrasure of mice to tire initiation ID of trilano shorred some hepatotordcity as evidenced by a rise in BGbT (Ccr.rang, '3 9c3). ASI 00004261 i r3 Ir.i : as lead:: s'- h - higUX' or.-. f> if".. - " ., J 7 j '. i ' *- > bras re : 1 -'.I't at Ou 1 1 " 1 V; A.-'a-U U' c' ;..." Cv " l*/` '- 1- / a e.a vr:f V of a! a- - , M i . ' 7 '_?,7 J : V I' V. i' i - . , i.. nh: . .. v . U * . r'*' 1 'c ! ' r* 1... i '' > , ; vs i-.-i-/- u cue v rd : r-j f. - , ' K.h. fv - ; *- - v. \S' - . La xr.-a - r* : (Data.' ,, e v;y-.; j.fft] --..''css . 5: i'.: a v -a- r, ^ '*_. r n on - ' I * :* i.- b'W : \ (VU ' f: a : In - - ' ' 1 ' i - , `L _1 t air (xt: .a-: 6 r 15ft) t jr: 1,` wl.' o t. fra . '. L ` . . act 05 * tt 1 iU _ -i\'j *, -A (9.1 l;aa. _. i" >, v 7 1 - o. *- } . m -`.n`; fit i * , . 'a aim ./ nor ll'i 1..0 u?l ; -t . A^.p.c j. Anii.,a 1 - .s Route w,LV), nl/hg w... y. : aht ID, j Reference Mouse Eat Guine-nri G Rabbit Dog inhalation Or Ct i.p. oral inhale Lion inuaiaraon s.c. fob ml a Lion percutaneous i.v. i. p. 11.0 2.2 4.92 * - > 20 - 1.9 7300 pa- (2 has) ~ p- 20000 37000 r-. 5\0..ia) 18O0 , . > 11000 - ; 150 von OeiGingsn.1955 Flan et al., 1550 Elucssen i- Flus-lTiu Saych et sL.,lc`tl A'i'-v'-- ot al,, 19"1 von C. Ltj.i.ren, 1555 Earsa-a. -*1 Sued .15 54 B err a: d i ct al. JL Smyth ot al,, l'>7Bar- cvr ci Sae,d, 15*.5' Klee seen U Fisa,15i Mice, rits, guinearigs end rabbits dying acutely from in::. 1 tion, show no toxic effects c;n the tissues, liver or kidney no:- after s.c, or . ,v. edminisi'ration (Brown: ng, 1965). I.p. injection of Z, 5 ml/Vg trilc-re in . :.foc had no e ffect on PSP excretion and produced no proteinuria or glyco.-v-in ".or histological renal changes (Flaa a Larson, 1965). Oral dopes of >f rf. eg bod;,-weight vere fatal to rats, mice and guineapign with signs of gastro-ir .stiunl irritation (von Gottingen, 1955). Chronic oral poisoning has caused -.ne liver and renal damage in dogs arid rabbits (con Oettingon, 1955). Acute human poisoning cases have recovered without hex tic or renal sequelae. After ingestion tntre is come burning of the oral mucosa, jo tor nausea and vomiting with vertigo, ataxia, somnolence, confusion, delirium mud u;-s (Browning, 1965). Excessive inhalation has been blamed for hepato-nephritis Lut the incidence is very low and ix is possible that liver and renal involvement ar-. ihe result of underlying previous disease (Roche et al., 195S). Untoward effects o'- the circulation, cardiac irreyolaritics and excessive capillary owsirw; with Inchy.,: -a tut no ndvew'o renatre effects huve been \epo?"ed after anacstnotic use (von uutJ: -.yen, 1955). Ingestion of 60 ml appeal's to be fatal in man (Fcbay-fKryroula et al., lyS6). At elevated tempera tuxes trileno reacts with aorta lime to form dichlor: , aiylcre ,and this reacts lurlhcr to generate phosgene carbonylehloiudo and various , ids which are all toxic (Dr.frlyre, 1961). The present fLV is ICO ppm, in Sweden '< ppm, in USE?. 6 ryem (Browning, 1965). Spies oleoresins my contain a nuxi.r.u-.'. of 30 pum,decaife'i::atcd ground coffee 15 ppm and doonf fcine.led soluble coffee ^Ivoct 10' rum (fjA, 96.3). A=;T 0 0 0 0 4 2 6 2 L .1'* n " jr:-;.;. ': '' 3 (I.1;-.;;; ,j.ou vita uioc-m.'vf' v. ji. rat caU v ' ;c- ...era in non and "roe avai"1 :'nlo b'V' f Kone avow j Obrcrvaib in v Is ( , Of S Aor v'.v" ir A ; ;:v_r V. up to ion r/nits . sh-a/ d: inn" i..b coo,/ AAon.' A3 'j , i----- ,. . - - ...1 `J,, v . u C-J.OC.VC 00 liver or Array or if . : :-A:. . C. V and gaugi cr\'.l dure; or:,tier (;; cr;v o'1"' 'capvg : r: io oI roan- rabbit . r.A d -ye vers c.xpcKid 0 to 3..:.A fm 6 VC :n i-fliiciv i Vic: !. oiv; ,e c i i g 0 .<' . d>.~. rmr to 189 m.g/i.:'' for 90 day a do jv/v-led aa si/nj Alcant p-.thoJ.og'. al ctnng ['u c c. l,, J 1967;. Groups of A j mice -.-"'e i-.:;'; ? r-i. for 1-3 vr.v rh to 2Cj or Ivei V';/..1 daily for 4 hours. Only r.-i.J.. '"i tr'rcic-:' t Tatty hepatic day1 aeration on.I no renal effects were been (bylin ot al., 1995). Guincani-s vo"'? crossed to van err of trilene fee 2v - /, month:' vit'^rui adverse effects on bodyvoight, hoe. .'vtoiogical findings or eririiysis m-su;;..:. but there /as sit rh i eviferco of hepatic parenchymal deroaoraiiar ml non;?. alone: .i. .r end tubular deferer.vol.co (l.-inio ct al., 193;). babbits fiver, for 1 - 3 ::.o:n 0.C74 G/--G trilene shoved livtle sdv: effect or oof yvoi riot, nioic"icnl finding. urinary analysis but see. h V.,t; ror.d. lesions were sc Amis eh a';., 1939). fa; were o.vr mod be- 139-750 pen d: ^: for 2-3 weeks. hepatic injury as ovidone-'d by EUP excretion, glycogen da; lotion and carercny; '1 defacer'.tier as veil as weight loss, lethargy and diu'-rioro. occurred cut cleared or stonnir-j c::u:a;c (boillcr, 1944). Soyabean real crime ird with trileno but not vith hevara or carbon tetrachloride has caused fatal refractory haer.orrharic apl'-stic are avia in. cavtlc (Pi ebon et al., 1953). Tha toxic factor was shomi to be associated vith t!io protv ir. fraction (Soto et al., 1953). Similar effects were produced by tui.lone-extracted moat scrap (P.ahfeld et al., 1953).. hov/cver, chicks fed trileno~e::tro.cted mat scraps shoved improved growth (Ballcun, et al., 1955). The toxic factor has been identified as S-trans-(dicliloicvlnyl)-L-cystoine, a reaction product of triler.e and protein v/hich becomes freed on protein hydrolysis (bcbinr.ey et al., 1957). Groups of 20 male and female rats were fed instant decaffeinated coffee solid extracted uibh t.ri3ene for 2 years at Op or 5/) of their diet (ocvivalent to a' residue of 0.5 ppii* trilcva in tt;e diet) without deleterious effects on survival, hehaviour, growth, food consumption, urinalysis, haematology, organ weights and histopatr.ol.vfical findings (Zcitlin, 1963). 3 males and 16 female rats wore fed on a diet containing f;' or 5b of instant decaffeinated coffee solids extracted vith trilcnc (onnivalm: l > a residue of ` B 5 ppm trileno in the diet). 2 generations v/ere studied as regards paternal and filial mortality, conception rate, resorption, litter sire, grewth and survival of litter. Organ 'eights, blood chemistry, urinalysis and histopaihology of the f0 generation were nor..ml (Zoitl:`n, 1967). ASX 00004263 /rc.it (.-) j A be: naoyerv'.o.LAy yu'-A' ;, -,-. fc.-j -fyn do cal.' aerf. l.-ala.-l 2 t/fc ir-llvo ny. . t' . 20 ih ' n cm- A : r..-. -, - v ..f. in. Ho :. A'- , . ; r\:' . = - r V-.'i :..' .. j'.:'. i, r. - !', , .... I'; :'l trib IO~] '.; A f; , -3. f ( A -.A '. :.J. r?- A 1 'ir.Ml-VLV. O!' 0 : OpV. 1.`Jim A '<' 1 .rvwy. TH.. taros il. in: 'it'';; r.mv"i, All "b) . hV. v. i;'c r-} io v'i ; io .'A"': ' rale i- cl !,rij.:y r; a an r'i?.;r'f: c for Mt.:' an.. A.:"T oAr ' ' : ' ' 1; . 'v. vl ' .1 c*C- A- i. . - " -J . . ^ (V 'la/,:!. l',AA} rail rocogr.' ." c.L> -' ' t;l or.! i :l v (ic oral A.'V.yl': o'A. advii o.t. "ko a v ; A1 o fAe'1' A. . 3957;.. la .j or to:? 2.1CJT5aw ... ,r ;.v \k;. ..: * v.'.O.<- - o,of h;,cv. afct.eglcai o/'Ao'.ta iiOuro <3: ;I'.- .v e r'l. 13.-or ; o :.rar;. ather:*.':: condif;.',': 3 vich rareJ.v apparent car'-.v.ac . .irym Vva:<' ..livial p;J sies and ootde r ^rve involvcMc:;'-!. my !o io>n rl: = to any :ritioe`. iut havu rot boon ro-n vji:h p.wa matr-rial. oA .1'...:'r:t; o-vl ('jOnotro-'inteotl ur 1 ry. ;oto'.:'.n havo boon rooortod all:r inauryr: '.1 o-"o-v._.y-.r'.r''.?. nilIcl.io'.i .bar Icon reported (bar:A`.` j A Vyekocil, 1956; Brouairy. ]Ao5; rrloy. 1913j ,rA.-.A:-,l.TJe; 3.961; ivlt; Kit.'hull 1 Parri0r3-.-Sr.iah 1969). iY.y-ly. ,'tov parformr-co J n not a{Tcct-:,3 by r: rosurn to 190 ppn but there is a doolj no in porj-omancc at higher iniialanion level:: (Itop.j u lIchAughlinj .1.967) o t \ ASI 00004264 r 12 :'..i , ' 2.0,, ' ; . :.'7v A6v: :v>. ,7 114. (rp) }: 1. p! r . : 6 V/.'.' C a.. ". /a...'. (10;..; ly;'' J;/o, y; . V., 163 J- (:?: -.) Ay , )"1: -.2.1:!. 2':, 7-A_ )?clv:.'L; ' .V-ytcr. (195") ?. ', IZU 763 bar." on ! I;., uni i c- (jA;.:-A) ^_yy_.-___ ' . . 'yc;! -, ,7,- 6.75 da; .. O''1. i3r.r: ord . v (,* 1 if ^y..r ;1' y-._. 7 -- , ..0; lyr , 7, :r.G Pyy'r.ynv J. Lyla, A'-Allv... a-_ddl2- -y.d. ; "9. 37-6 Bc::.:r,7. P., Pyuan., and La.'ori, P. (19:4) Ipy ... .'.'.It. 7;2 669 Uxc. 1 (1915) 1;7-1'1' J'LyJ-A-!' 373'' TYA 93 .; 4;7bi:di.;Ajy4A'dlr;:; -J sy.Lnr, Ansi. .'dun Butler, 110. (1949; .by.2 yy: -Ah i . .y. . A y Byiuyteu,. K,H. ana .yi'yy. .'..7. (IdOt) yyny.-, I, 2.(7 Clay -iii; J.l. and PnrAvuay J. (1362) 4n:l L. 6 -_y ?:-' ., 4a, 1/1 Corn!eh, 1.14 and 11-44.;. .1. (7.946) 2:y7-,22'.U, t(yyi,..2.., 17 Daniel. j,;-.'. (l957n) yrayiy.iy/y In. ill./ a77 Bandy, J.VI. (19371) '-'VP danicl, 6.9. (1963) 14 y^' 4 ' a ?_9, 795 0 Defalruo._ R.J. (1961) (A iyJy:AAVyiL_.yAy A-, 2, 665 Derviliey 1., ''ur<, Ch. and Oasis, (1930) 7191 jyt.Oonrr, Undallyd labre, ii. and Trukau t, It. (1956) 4,,_La.'l4lli.r.i.-41-? 9, 39 Pood an! 7t.'.G Adulnistyation (d'X:A) .111:_111, i'4'7. Gahyny, P,,J. (i960) T or: - a1 . a--.;-? . rr -. me a -l.. 1?, yyy Guyon-i cur.nin, G. rend Van StoenkiL'tc, J. (ivi'd) ,-.rch. ) a! a-'. Prof-an., IQ. 439 Helllvel], P.J. and Hutton. A.A. (1950) AyoidiTy 5, 4 Johnson, M.K. (1965) Ivoohe:-. ?hn--ypl, V, 1303 lClar;cron, C.D. and Plan, Gy. (19690 Toricri. r. .nl. ?har-ncol. . 2; -99 Klaaasen, C.D. and rlaa, G.L. (1967) Tori cdl, rn~U. lna:::anrl.i Tip, 119 Kylin, B., Suneyi, I. and YUner, 6. (1965) Aci-' "-ham-'cl. 22, 379 Landc, P., Dei-villc-e, ?. and ihn:, Ch: (1939) Inch. ioToss. , 7, 454 Uchinncy, L.L., '.Jeahloy, P.B., lldrldye, A.C., Canpbell, 7.1., Govan, J.C., Pickon, J.C. Jr. and Bieoter, II.1. (957) J. Aner. O-en. Soc. 72, 3932 i-S.lby, T.H. (1962) J. Occud. i-led.. 10, 252 i-dtchcll, A.3.S. and Farsonc-G.dl,n, 13.G. (1969) B.M.J. 11, 422 iiococ.hlin, 6. (1956) hl.inik_-l Tls^-nle agr 7or~j.p unmn. Th , Stuttgart Patty, l\k.. (195S) Industrial. hy"\c-ne and To;n.colo'4. Vol.II. 13C9 Pcbny-Poyroula, F., LeGall, J.P.., I'rl j-ville, J.P., PoscnOi'.al, 9. Ci Gaultier, K. (1966) lull. Soc. i.ed. };ar. laris. 117, 1137 Ficken, J.C. Jr., Jaconsen, b.L., .alien, P.S., Eicstcr, H.I., Bennett, P.C., McKirj'.ey, L b. and Covan, j.0. (1955) -rri.c. Food 01c---. . 420 Plan, G.L,, Ivans, 1. A. and line, O.H. (1950) J. ~r. / col. l~:nt. lhen-r>., 123., 224Plea, G.L. and 'narson, 3.2. (I3c5) Toxicol. .'.n-4, lnr.--:c.ol.. 7, 57 Prenderynsn, J.A., J one.., 3.A., Jen,.ins, L.J. Jr. au.d ^ieyel, J, (196?) Toa-iooi . An-,],. Ph"rr a col., IT), 270 hehfeld, G.d., Pei.nan, V. , Buutler, .T.H. and achultao, .0. (1953) A arig. Food. Chon., 6, 227 Roche, L.. Lejeune, a. and Hi Pi at, J. (1953) .-'.nr. lied LoVnle Crird.tr'l. Police Hci. Tonlcrdl., a' 336 1 7a .'i > / \ '1 t,.'sf. ^cr. r AS1 00004265 1 .j : { Soto, Y.4.. ;'"rl Z.-h'-]'!.'~. ' ! r-- 6,- (r;.M) ; - ' i ' ; * . 1 -1 vJ . - .1 u ;.. ` . U 1 1 > . ; * v' *t *- ' / ij /, - > v-~; Go- '"A;;:. :5. , ; ' 1 Gi.c.t Sv. -A, A. "j. (V, `'' - -- ` J- .* r) s '' ' 1 ` - ~ ' r - 1 `v 1 ; ;'V7. - L, 31 - l. (A'; ' 1 1 `.._J J , .Iv '-3 7 _ r i . (196:) ^ ; V ! ' : ' ' - *6. , i. . U. . m . .... S^OT).,,. C..;. : ......... MV. Uv-4 ) /- . ' ::6 .i , J.1. 7:.. : J. trz\ :, 6. (,19c'.-,; j 1 v \ . I-' . _ ,-cun. Vi--:v.. IT; /V. ' ......... ' Uhl; G. 1: (iGl'j) lyyh, V . y -1. . VV, 97 Vir.lllf.r. J. or1 , ?. (Hoi',} - V'c-rvl, lr - 4 : . . TH, 2219 von Orilinyon, 9.9. Ovr-lZ>) U.S. 'hilli.r :.:n; i:,T. (l;r?) y /....>V t.*1;1 4 .V:1. /V y ; -.11; Jo!''' 1'ij.oy V L-.-nj, i'tw Yo:::: Zei'-'-iin, B.K.. (I'.'O) }~i v:y Hoy;-J 6" : y. 0 rr~ ;.-'"L'tlr,n pyh: ! It..' to :0 Zeitji.'i; b.r. (?. v) 2::ixI.VIhn-;J3 ::_1 .i'.'.'G ; o. f ,y.-3 huy ~i, t n to WO Zoii!:.n, (. j. V - - J ^''S'Z'u ''> Z'<='`j[;v ' : o' 5 Govt-' on, n M'r-M-i ! i., tn 'll PowelD, J.T (1746) gin., ..vr.": :.:T7 imr ` k V ASI 00004266