Document zoJQOan1rJO6mYRny6ewjJnb3
AR 426 _ O82
TOXICITY TO AQUATIC PLANTS
IEsTsussTANCE
00000000
Identity:
A mixture containing referred to as PFOS,
perfluorooctanesulfonate; may also be FC-95, or as a component of FC-206CF
or
FC-206CF-X. (1-Octanesulfonic acid) (CAS # 2795-39-3).
2R0e6mCaFr.ksC:urreTnhtein3fMormpartoidounctiinodniclaottensuimt bisear
was 101. The test sample is mixture of 0.5% PFOS, 10%
FC-
d1i.e5t%hysloedneiugmlyoccotlylmosnuolfbautte,yl1e%thseurl,f7on8a.t0e8d%alwkaytleert,h1er.3sa7l5t%s, a6c.ry0l%icufroeaam,e0r.,5%
triethanolamine, and 0.05% tolyltriazole.
cThhaerafcotlelrowiiznegd scounmcmenatrryataipopnlsieosftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicityof the fluorochemical component of the test sample.
ME0 THO0 D: 00000000000
Method: OECD Guideline 201
Type: Acute Static
GLP: No
Year study performed: 1991
Species: Selenastrum capricomutum
tShoeurUcniev:ersIint-yhooufsTeecxualsturaet.AuOsrtiigni,naTlelxyafsr.om the Culture Collection of Algae at
Element basis: Algal Exposure period: 96
cheollurcsounts
(cells/ml),
growth
rate
Statistical Methods: `computer program of
Statistical analyses Stephan, 1983.
were
performed
using
the
Analytical monitoring: pH, conductivity, and temperature
Test Conditions:
-
AmlegdailuNmutprrioevnitdeMdedalil ummin:eraNlutrnuiternitenmtesdeisusemntpiearlUf.oSr.alEgPaAl,gr1o9w7t8h. aTnhdisalnsuotrient
served as the diluent for all medium was 8.0.
algal
operations.
The
pH
of this synthetic algal
wSatoscpkraepnadretedstinsaollgualtimoendpiruemp,araantdioand:deAdpdriirmecatrlyy 1to00t0hemegx/pLossutrocekvseoslsuetliso.n
sEoxlputoisounraendvecsaspeplse:d
250 with
minLveErtdeedngmleaysesrbefalkasekrss.containing
100
mL
test
Agitation: Continuous
Number of replicates: 3.
INnuitmiablecrelolflcooandicnegn:tra1t.0ioxns1:0
cells/mL.
five plus a
blank
control
001899
Lighting: Continuous lighting at 44 uEin/sec/m" using cool-white fluorescent
Wlaamtpes.r Chemistry:
PH range (0 - 96 hours):
.0-10.8 81-83
(control exposure) (1000 mgiL exposure)
Test
temperature range (0 -- 96 21.9-22.3C (incubator)
hours):
R0 Eswt0 s 00000000 Nominal concentrations: Blank control, 62.5, 125, 260, 500, and 1000 mg/L.
96-Hrs. Algal Growth Response values
Nominal Exposure Conc. (mg/L)
Cell-Count (cells/mL)
| AGvrgo.wStphecriaftiec | % ChCaonntgreolfrom
[625 1 63000 |
0.043 0043
[= |0|
[2 3040005 | 000.300 3%7 |[ 1 167|
[800 T7000 | -0.003
1000 [o 000 T >f0 |
Element values: 9966--hhoouurr EN,OCsEoC==25642.(52m3g3iL--.277) mg/L
Element values are based on nominal concentrations.
Reversibility of Growth Inhibition: Not conducted.
SRuebmsatraknsc:e the fluoroc
RTeesmtairnkg hemical
swafiseldc.onTdhuectveadluoens proportion alone.
trheepomritxetduraeppalsy
dteostchraitbemidxitnurteheaTnedstno
t
_
c0 oncl0 usi0 ons0000000000 The test substance exhibits a 96-hour ECs: of 254 mg/L with a 95% Ccoonncfeindternacteiionnte(rNvaOlEoCf)23w3asto6227.57 mmggl/LL.. The 96-hour No Observed Effect
Submitter: 3M Paul, Minnesota,
C5o5m1p3a3ny,
Environmental
Laboratory,
P.O.
Box
33331,
St.
p0 ataQ0 uagr0 y 00000000
001900
tReesltiianbgi.litHyo:weKvieirm,istchhersaankmipnlge=pu2r.itTy hweasstnuodty pmreoepetrslythcehacrriatcetreiarifzoerdquaanlidtythe study lacks analytical confirmation of the amountoffluorochemical proportion
in the solution. This test was conducted by EnviroSystems Division, ResourceAnalysts,
IMnNc,orLpaorbatReedq,uoefstHanmupmtboenr, JN1H884at-4t,he19r9e1q.uest of the 3M Company, St. Paul, Last changed: 6/26/00
001901
)
:
i. Sis
BEST COPY AVAILABLE
snestssemnttsot reer
0 the Alga, Selensstrus capricormucun
Frvm sesmc,maam n
.
i
|
iin
i LE eanEhi
Sa
Es eons
La a
oo.
Ce |
oe
La
po
a
a
|
}
;
*
<
)
CLT
i
)
001902
BEST COPY AVAILABLE --
HBETETDeatelP rSs2,hc=T yI otNrrJownsAlto
ve
sin, nisi a wr 2
:
ScEEEmEtHSWTh WHPTIELShfteueee ci ote we ce
}
S H Setm? n Et , SeTpR eMne Light was adjusted to 44 uEin/sec/a' with TenTet a photoperiod of 24 hours
~~
SS sIeEl nAlgaLe Iumswed mTin SsthIe Litest TBTrlesfeT eAsitt e was from an in-house culture that was
B iEeRkSge S s h mS
rR i
ERT CES ASEEER Sar
Sa
ee
Hog
THRE
C SA aUARTIEEL ee
nceirlSa at ae
pe
oi UR
sa
Gull ie)
ie
ES
ESTs
Bead shin RA ls
BE
Ra
-
iE
remy i
fl 001903
= BEST COPY AVAILABLE
i
BE ;
?
5
Le
Er
[RR
TEE. aden ot sles
wr. eeion
[RS ------
nemite
wel main
Aopen A atesGutsy ca from ToroiTee y
Sided
"
s
.|
.
.
.
-
:
ae
Be
is or a (EE
SO
Sag
ee
|J FRe E ae Eo Ld
RR
ho
EE
he ah
I
PetEE
3
-
.
k
TR
j
x
001904
BEST COPY AVAILABLEEre of ise
oe 1. hBemteLnt aeetcetseeiosn ovfis& pevnesoive &
Table 2. Growth data fromtosicity test
~
0
:
i tabled. percent champs fromcontrol aad average
wi nite 0. psnstein conection on
u
ll 2. Tepeeeace semees uri Snieiy sot "
1 nie hteoh amendantag sod ne
-
a
Ee
;
Ee
Aare
L ad
oa
cme a ee aoOaER
Ca a 3
Bae LC a
RRL
z
RaE se on
se
PCa E RC R
RR
i3
|x
Nae
reas 56,1800 0 A
ln ONE
EndE
a
or
ee
CT
i
-
N
WE
001905
BEST COPY AVAILABLE
e4 pertTehrivinatUaOorTt.aa"nTh
wv. rection fsenepr?oeSnAbayIs3Leocaoamrpunrsy,
oSti.ecmpiiala,y,oNrifssTmsoeodrdsl,eerTeooe
r IE
ha aes Tey eetea aleien tre
Sr rt
"
mer sonsoances SrIheoarnsttreodnt"yTeriOemITsToloeinsd(imyibisErohot3ny1sh0iTee0"om le)SamiTpaeletercMhnTaeibaeoemtrsetidone191n3a8) SiSawtparieEeSLssaetrribiereaeaceestd oestno "E30 ba reeked a e imieabT yereae tfotrermelnismeprliy (fp77Teoe n1er18
SrsoireteetlHoarwiyieerdesosTtneettedeiaadnforUTwitsotceelhinstoirEonLhBoifRetHIerRsAeaarntegshnSntaEdo0)h nf1sofSaarite nsoxoantieetoiteFyy
--
SSha, LSHBiAEotSa uCR roeldlcftoirD ontheoftaAsS lveasU ktotohn StcecuiT rnnsopA SifmaaltI lt]y TporCN of curedS rfro
T 3 0 R
T ee eer ieee mi
renem aenm, GSEe) HG ag a aeb
mie< Smedhors AE
L B&oCEiaeiL S n a e e onsT eT n aS H E Re SS h a CeeasvseatieenssuencoclonncdeuedcV eitosTee2R d4 S2a oe H?E.2
$ Lb EYnSIEa n T h GFeEeee en tT teeSrnooffe r eTeeeWSESERaEo
i cAa R a DEm A xa S raaa S Be eki a Cenaa =
BEBa ae a a Shan ao
,
ps
)
q
}
001906
yi
"BEST COPY AVAILABLE
)
OIE mn, Seen or ST aT
--_ Parameter
eeUnwtit moefe TRepETortERingT
WR Measured
)
Sooxposiorioe wgn.
'
-
:
rr
os
-
E
ess
L _--R
os
%
rop----ym
pa eg Ve TER
5 aga
Gane
rh
Lt
forge
GEES
SB as ible orS Lo Saka
EBRER a ame
;
.
ARE[AE SlGsRaEAiGaRitEysAT
rE LomeI
8 RE iNir Sl
:
i
La
pl i
Pet2t :
A
-
EGEER EL aaaa d
OpSE iS tsg AOS E
SEVERE
i AS
et,C|AN
cE
BEST COPY AVAILABLE
STTS1o1io0Oran0ec0Leo,imLEeiarmrasvvLwiiatoaehn"tosruotLcoSakfoihmeMeutocahtaniaelttedianocsuneuotlsye8lvt4aattnoSeapsreegoamaealassetedmo-pnetrnseScsaiuhsrrieeinCoe,fse2Ta2cr+ehr2,,4SRwiTiEhesehEhsLFhio,vcAfkeire
Soraprannel ere? Sal LR, GRahmimerseSoenaCtienntendotehe Ctaent
}
oEsonptEtopnetMaroriveoodr Tons Semele
win"eeenesr c(EoLyaLlEaRsp ,AtreWs1mrh0e4n,dg eSwTehRaeIielNay aySsiitghsicrriCindmeieoessrlaammeoonFghnessdehenenceetns
GJinoutcretintosie niotnyChSoetf 4"04"6euuScrohelrulasea3ahst)L(4 XSe3n4dehnomwee eTitgsheAooEn eoeheeee,lle,Sienmeiseekecetsh.e
:
SE TeimrroncRirtioyJiemr,neetresferussbSerRhCe neo"frhe oameellgyra(lamer cbaeinlsialse/msap leoofeitnetnesnacad h"SettnTehsteeervoeeensnselsl vK eCanosdndeietateervernimriitennhee8sdd
e R Tee mlte Ioe Cf She ta otxiecnietty f1teesitcriwanrreeahinrateeirp,reyCcFe,asllobeyfsyssreavemrttnrceea
STt Do tedepiStivoeitmdamneteedere"sberonat Tciho"ee.f roSmurhealu"henniheedeDepnrhIeolitTlsieso,enesssoeTaeatHeSpuAcRes,IerEprneaireinstReCcmoLraehu2TwiaeT e(cLhiiycnneien dteuenrdi5nogF
foo
i mE T rRr T eSo S LLsE L o ER T C s2 T 8Bours
3 ,
Bam OST
RE
THR TRE
ig o AarseaoF 5
EVM EE N SSEuaa n
FMT
iSEEa i pe sh
lg ie
seb
TEN
IpRdA A E TNERE RetRiR Ehite v4g
FB peeya ash osm
a
ile
EE
a
Lf
ds
Li
TIER A
i
El
-
:
'
001908
rit
-
BEST COPY AVAILABLE vi. nasurs
ha
:
BR
--
E rn L SIT DLT RR
.
: LE
eEeTHeldT f Tet eoe ge T3 2
|
| S Ecoe Lg A E a o E Se AE E amao
Hi a J gg eCRe
Tjaasle is
E
.
}
;
"n=
001909
rie 2. acon ut trom snteey ne BEST COPY AVAILABLE
e-- e ----------e----
S cNoewamtnnmalis weno Ie TumbeOr oBfwCCeT llleswpmacMKiillndiieten r1x1010)w O(hoour))
.
"aod0B 3 moomoo
- xR % moo=
;
"6 BB x =
L bR es 24 8oe m EwEFom 8
in ee
Bp Hed RET
8
fora ES
een 3
po
Ranson 12 Sradag 0 ol3,
|
fl ea
hme BR EN EE
Bi FacChl
2 C 0RRa PRCRm M Ls
SE nC i
Ae
Een
EEE
a
freEe
ih
Bono RO
BE ee
0.
ae
he ate Eo
:
ER
ie
feos
-
)
I rr
001910
Tie 3. eErsmhr rm coe a sce sei armen ue
:
hmNIe oimina,l rAe vereagme e spveiticee TeoePeesrcemeente nchangee
: ||
;
#
i.
es
1
0.06 0.00 0.083 0.05
00m oom oom 00m
11 5 0
wm 3. ae
250
0.035 0.029 0.030 0.03 - 27 46 35 16
o--on _----soit eaT e moE ci T7 sm e um -in
i
i
La
il
i3 4
gon a
BEST COPY AVAILABLE
0 ae) ;
5d
Sid
5
BE
vod,
ae
iden E
asp
Es ee
Bote ol Ba ia
; |= Ee a ae
R a i f SR aE EE nl pg Sa E
i
)
PieE
eam bes
8
SN
001911
rusia a. Medien attective concentzations (ECS0R) from toxieley test
i
p
Te a Efe CEE
ee men mrmen wn
:ke aenn mwimmaan
i EN iyi,
BEST COPY AVAILABLE
Ere
ied
EA
Suge
Daclt
&
pga
EETTA 3
Boi Oe
sg Lyergadl
||
SE
|
i
E
oa
LEER
faa Cd a
fi HL Le
ia
oh:
AL
Shani as
UR
B is e ena a
SEM
eps TY
.
:
g
|
:
VII. neracas
ip,RL SLT St er
Seapnan SF 8. computer Program for calculation of LCSO values.
)
Skise To Aoan Se e aprienn prints Monk Assy
}
BEST COPY AVAILABLE
|
ma fe
he R Phir TE pe
eL an e S aI eE eS
Ei
Ebi Co ERE
El
-
)
IE
g
001913
BEST COPY AVAILABLE [TV
2
i2
i Oat47
dLiaagy
Sa U a EJEang aba
A rE
Reale
aio
F od a fea i
7
Ee
fo Coa
TRE
Sa ED
f s
RSL ana
PON Py
4:
|
Ei
fboy3 aE
7C%2E!
[i
.
.
I"
a
001914
|
i
EE
_
rsuiese ABSE; F Gove agp i
3
2.
;
og
)
*
2.3
--
-_--
i
i
Ren
Gadi
AE Sablah SO
a
Rtn
4
PIs
GuslERes bnlEE ee es Se
Bn a
f|
E BaEE me
aa eo
]
VEN ea
;
J
hae . 4
CCN
Es
-
ETI
B
001915
2
y
Eg
.:
er. mene iy ners COPY AVAILABLE
EEeT)
seisons
=
nam
2 tpmtuety as
3
:
.
a0
10.8
pes
06
B
a
;
noua
:
0 0.
in
3
a3 3
ioo0oB OH
i
=
:
Pr
i FE Pn Wn
aR Paks tm
ie Selah SERRE
Se
fb sn CERN SR Ra ERE hr
4 Sy
bi iE
i
VRE
a He a ae So
Wie a
e Cy a ACR CE
[Es2
|
--_--
.
I" :
2
CE CEE LTR
fe
i
R