Document zoGLNQBDzp6OovKY9O926rOv0

AR 226-0084 TOXICITY TO AQUATIC PLANTS (E.G., ALGAE) FE-2 TEST SUBSTANCE Identity: PFeCr-f9l5u.oro(o1c-tOacnteasnuelsfuolnfaotnei;c macaiyd,a1l,s1o,2b,e2.r3e,f3e,r4r,e4d,5t,o5,a6s,6P,F7,O7,S8,o8r,8heptadecafluoro-, potassium salt, CAS # 2795.35-3) 3ReMmlaort knsu:mbTehre21te7s.t sSuabmsptlaencweaiss astwohrietdeupnodwedrera.mbiSeanmtpcloendwiatsiontsakperniofrrtoom etelsetminegn.taPluraintayldyesteesrmteicnhendiqtuoesb.e 90.49% by LC/MS, 'H-HMR, *F-NMR and METHOD Method: OECD 201, Test: Static acute OPPTS 850.5400, ASTM 1218-S0E GLP: Yes SYpeeacriecso:mplSeetleedn:asSttruudmyccaopmrpilceotmeudtu19m99. Report completed 2000 oSfouTrecxea:s aOtriAguisntailnl,y mfarionmtaTihneedCuilntcuurletCuorlelmecetdiionumofaAtlWgialdeliaftetIhneteUrnniavteirosniatly ALtnd.a,lyEtaisctaolnm,onMiDtoring: PFOS measured at 0, 72, 96-hours gErloewmtehnctubravseiasn:dRegproorwttehdrattheree ways: number of cells/mi, area under the SEtxaprotsduartee:p4e/r1i2o/d9:996-hours. End date: 4/16/99 Ahnouarlsy.tical monitoring: Test concentrations measured at 0, 72, and 96- gTreoswtionrggiannfirsemshswaltaebroraaltgaolrycuclutlutruerme:edAilugmaefocrualttulreeassthtawdobweeeenkasctpirvieolryto gtersatdineitcihaetimoinc.alSstotcokrenvuterriseentossmoolsutiiso-npsurwiefrieedpwreelplawraetderb.y adding reagent- Test Conditions: Test temperature range: 23.6 - 258C 000601 Growth medium: ASTM Standard Guide 1218-90, 1990 Concentration bacoso |ww |op| f|a aw s |me| boo | owe |ow| hose | owe | mg | fo | aw |ou | econ |os | we | hos|ernono|iune| oe | won |un| hue | oowm|ampn| hoo. | mo |og| bo |ur m |e we | boeo | tou | me | bT oc so | mg] pDuirliuftiieodnbwyarteevrerssoeurocsem:osWiisl.diTfeheIntteesmtamtieodniaulmLtwd.aswepllrewpaatreerd by `adding purified the appropriate volumes of well water. The pH of the stock nutrient solutions to medium was adjusted (0 7.5 + 0.1 using (0.22um) 10% prior HCI and to use. the medium was sterilized by filtration wSatoscpkreapnadretdesitnsaollguatlimoendpiruepmaratataiocno:nceAntprraitmiaorny osft1oc8k3 smogl/uLt.ionThe aprpipmraorxyimsattoeclkys2o4luhtoiuornsw. asAfstteirrrmeidxiwnigt,h tahemapgrniemtaircy ssttiorcpklastoeluftoiron `waaddsitpiroonpalortteisotnaclolnycednitlurtaetdiownist.h aAllglaflinmael dteisutmsotloutpiroenpsaraepptehaerfeivde clear and colorless. fElxapskosspulrueggveedssweiltsh:foStaemrislteo2p5p0ermsLcopnotlayicnianrgbo1n0a0temELrolfetnemsetyer solution. Agitation: Number of Shaken continuously replicates: three at 100 pm NInuitmiablearlgoafl ccoenllcelnotardaitnigo:n1s.:0sXev1e0n cpellulssa/mnLegative control plus an abiotic control at the highest concentration tested 000602 Water chemistry: pH ran7g.e5-(08.-196(choonutrrsol) exposure) Test te7m.p4e-r7a.t5ur(e1r79anmgge/(L0e-xp9o6suhrouer)s) 23.6 - 258C Light levels: (0 -- 96 hours) 3870 -- 4610 lux from fluorescent lighting continuous cool-white Methodofcalculating arithmetic mean mean measured concentrations: RESULTS Nominal concentrations: 183 mg/L abiotic control, Bk control, 5.7, 11, 23, 46, 91, 183 mg/L plus Measured concentrations: Element value: <LOQ, 5.5, 11, 21, 44, 86, 179, 169 mg/L 2244--hhoouurr EECCssoo(c(ealrledaeunsnidteyr) c=ur1v6e3) (=741-21291()19m-g1/7L6) mg/L. 2484--hhoouurr EE/CCss)((cgelrlowdetnhsriattye))==81136(7(23--0 -902)04m)g/mLg./L. 4488--hhoouurr EE,xCCssoo ((garroewathunrdaetre)c=ur1v4e)2 =(18047 (-6178--5)1m4g6)/Lmg/L. 72-hour 72-hour ECyq (cell density) ExCro (area under = 37 (<0 curve) = -- 64) mg/L. 46 (<0 -- 56) mg/L 7722--hhoouurr EE,CCsyoo ((ceglrlodwetnhsirtatye))==7503((4243----7684))mmgg//LL. 72-hour 72-hour ExCso E/Cso (area under curve) (growth rate) = 120 = 74 (103 (55 -- 82) mg/L -- 132) mg/L 72-hour 72-hour ECso (cell density) ExCoo (area under = 153 curve) (130 -- = 165 165) (145 mg/L. -- 176) mg/L. 72-hour 96-hour E/Cso ECyo ((cgelrlowdetnhsriattye))==4>917(493m--g/50L)(Cm.gI./Lnot calculable) 96-hour 96-hour ExC1o E,Co ((garroewathunrdaetre)c=ur5v9e)(=544--9 (40 63) - 50) mg/L. mg/L 96-hour 96-hour ECs (cell density) ExCso (area under = 71 (66 curve) = -- 71 73) mg/L. (67 -- 74) mg/L 96-hour 96-hour E(Csy ECs (growth rate) (cell density) = = 126 137 (115 -- (105 -- 138) 153) mg/L mg/L. 96-hour 96-hour ExCoo E/Cgo (area under curve) = (growth rate) = >179 145 (125 -- 155) mg/L mg/L (C.L. not calculable) 72-hour NOEC 86-hour NOEC (growth (growth rate, rate, cell density, cell density, area area under the under the curve): curve): 44 44 mg/L. mg/L 000603 All element values based on mean measured concentrations Statistical methods: Cell densities, area under the growth curve values, growth rates and percent inhibition values were calculated using "The SAS System for Windows", Release 6.12. These values were then analyzed by linear interpolation using TOXSTAT Version 3.5 to estimate theEC, ECs, and ECy values and 95% confidence limits at 72 and 96. Cell densities, areas under the growth curve and growth rates at 72 and 96 hours were also evaluated for normality and homogeneity of variances using the Shapiro-Wilks's test and Bartlett's test, respectively. The treatment groups were then compared to the control using Dunnett's test. values. Results of the statistical analyses were used to determine the NOEC at 72-hours ranged from 81.9 ~ 105% of nominal and for samples taken at Analytical Methodology: Analyses of test solutions were performed at Wildlife International Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the concentration of the test substance in the test solutions, the same and most prominent peak response for perfluorooctanesulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The The mean percent LOQ (limit of quantitation) was recovery of matrix fortifications 0.115 mg/L in this study. analyzed concurrently during sample analysis was 99.1. Samples collected at test initiation had measured values from 82.0 to 98.1%of nominal. Measured values for samples taken at 72-hours ranged from 91.7 to 105%of nominal. Measured values for samples taken at 96-hours ranged from 90.3 to 102% of nominal. For the abiotic controls, measured values for samples taken 96-hours, 90.3 ~ 103% of nominal. Summary of analytical chemistry data: NCoopmnicinananToens,t || Moo"suorue.d oReespsac1i0e,h7y2,maadt 56-||cMoetrsormned, [e[oe|m--mmweoe imcsaco ||wm o0o|a | &||| [ F2 o| Twe esw n | | 0wu e |oowu ] [oe | ermses | ow |w| Loon | sain | 0 | | 000604 Control response: satisfactory Biological observations after 96-hours: Concaomiratan, [o[faCote par |InDhboivnontvi|iAereis UnGndevra|GnrioasnRot o= gmn cone |stom | |_|| [es [ow Toma | wr | we |4| Teomm|an | as|on| 5 [sowow Jomo | | we wo | | a0|a | wo | a0| we wm [lwoewr||oown | nw | ow | |on | | aOgbgsreergvaattiiono,nsf:loAcfctuelrat9i6onohoruarsdhoefreexnpcoesuorfet,hethaelrgeaweetroetnheo signs flasks of in the negative control or any fest treatment group. In addition, there were no noticeable changes in cell color or morphology when compared to the negative control, although a 179 mg treatment groups. few cels appeared enlarged in the 86 and Reversibility of Growth Inhibition: The 179 mg/L treatment group was maximally inhibited after 96-hours. Aliquots of the test solutionwere odiblsuetredvewditihn atlhgealremceodvieruympahnadsec,ultthuereedfffeocrt foinveadlagayls.grBoawtshedwaosn ftoheungdrotowth be algistatic. CONCLUSIONS three calculation methods. By cell density, it was 71 (66-73) mglL., by The potassium perfluorooctanesulfonate 96-hour ECso and 95% confidence interval for Selenastrum capricornutum was determined using area under the growth curve it was 71 (67-74) mg/L and by growth rate 126 (115-138) mg/L. The 96-hour NOEC was determined by Dunnett's procedure (p < 0.05) to be 44 mg/L using all three methods. No signs of aggregation, flocculation,or adherence were noted in anyofthe test aslogliusttiatoincs. or the controls. This test substance was determined tobe PSauublm,itMtienrn:es3oMta,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St. 000605 DATA QUALITY Reliability: Kiimisch ranking = 1 REFERENCES This study request of twhaes3cMonCdoumcptaendya.t Wildife Intemational Ltd., Easton, MD at the OTHER Last changed: 5/3/00 000606