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AR R26 0698 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TESTSUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9lu5oorrooacstaanecsoimfpoonnateen,twohfiFcCh-m20a1yAFa.lso(b1-e Octanesuifonic acid) (CAS# 2795-39-3). RiseFmCa-r2k0s1:AF. TChuerr3eMntpirnofdourcmtaitoinonloitnnduicmabteerswitaiss anomtixntoutreed.of T3h%e PteFsOtSs,ample 81.82.55%9%Acdriyeltihcyfloeanmeegrl,yc9ol%bsutoydlieutmheorc,ty3l7s.u8lf1a%tew,at0e.r3,%1s4o%dieutmhyllaeurnyelgsluylcfoalt,e, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole. Tchhaerafcotlelroiwziendg scounmcmeantrryataipopnlsioesftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical component of the test sample. METHOD: Method: OECD Guideline 202 TGyLpPe:: NAocute static YSpeeacriceosm:plDeatpehdn:ia1m9a9g5na Supplier: In-house cultures. Originally obtained from U.S. EPA-ERL, Duluth, MN. Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 48-hours TSteasttisotricgaalnmiestmhaogdes:: <N2o4thaopuprlsicoalbdle dueto study results. Test coDnidliuttiioonnsw:ater: Carbon-filtered well water (3M Well #2, St. Paul, MN). Dilutionwater chemistry:Not given. LSitgohctkinagn:dNtoetsgtisvoeln.ution preparation: A primary stock solution was prepared by dissolving 1000 mg of test substance in 1000 mL water. Exposure concentrations were prepared by additionof stock solution to each vEexspseols.ure vessels: 100 mL vessels containing 50 mL exposure solution. Numofrbepliecatres: 4 Number of Numberof dcoanpchennitdrsatpieornsr:eploincaetep:lus5 a blank control Water cphHemriasntgrey(d0u4r8inhgoutrhse):study: 8.08.1 (control exposure) 000298 Temper8a.t0u8re.1ra(n1g0e00(0m4g8/Lhoeuxrpso)s:ure2)0.2 -20.4 C Dissol8ve.d38o.xy4gmengra(ncgonetr(o0l-4e8xphoosuurrse)): 6.9 -- 8.4 mg/L (1000 mg/L exposure) RESULTS c--r -------------------- Nominal concentrations: Blank control and 1000 mg/L. Element values: 48-hour ECso = >1000 mg/L Element values based on nominal concentrations. Remarks: Substance Testing was conducted on Remarks field. The values a mixture reported as described in the Test apply to that mixture and not the fluorochemical proportion alone. conclusions The test substance 48-hour ECso was determined to be >1000 mgiL, the highest concentration tested. No mortality was observed. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking = 2. This study meets the criteria for quality tleasctkinsg.anaHlyotwiecavlerc,onsfiarmmpalteiopnuorfittyhweaasmnooutnptroofpefrlluyorcohcahreamcitcearlizperdopaonrdtitohneisntudy solution. REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMN2C9o6m4p,an1y98,5.Environmental Laboratory, Qs UHE-- R, -- ee --eeeeeesmsee Last changed: 6/26/00. 00029 |. . BEST COPY AVAILABLE---- wn sams [A -- A FC vee est: STATIC X STATIC RENEWAL. Wi EN ICR CARI b5e8 oilunon WATER: `CARBON-FTIOLTWE.R2E0D4WELLHWAATERD(3 WELDL#O2,ET. PAmULL,180) Pia id 8 oaDAGE: asrROLONEOWATES NO. OF DAPHNIDSTESTCHAMBER: 55 [i mane seman _rrsounouous smu sasorervesa|s io le owen, gals sao oaTeTemeond LSoIp AsT NE Zz TOTALTGR hi ER VESS | aE HUT PEs e Be T a Bz _ |cToenter]o T roo--J-o_La&iL ihFeE)hi Eo iBkp Cl sa | c[ocuommmoerr| --|S ETEERERE Con oe TEeey i o- |a | o |g} ylyp-|lEaadtieesd ia bil | 8 eda R CAR e E a : [a AGU L aa E Bn aE Ra BESS sas ET TRA| RR | Lt me SS ERE SEE 0 REG EE le i (Rv|SRe a 3 i SEE TI LT f iE i. NR li a tS ront[icreeosT oe niP . I 0R >y1E s00YryS --S (OP D--S A --e R --Y --Y --a ---- ;E] won Gly, 000360