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ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic PFOA ammonium acid, salt, ammonium Ammonium salt; may also be referred perfluorooctanoate, PFO, to as FC- 116, FC-126, FC-169, or , ammonium salt, CAS# 3F6C2-51-4236.-1()Octanoic acid, pentadecafiuoro- 1R4e3m.arIkt'ss :puritTyhweas3Mnoptrsoudfufcitciieonntllyotchnaurmabcteerriwzaeds,8t3h.oTuhgeh tceusrtresnatmipnlfeorimsaFtCio-n ainnddicCastepseriftlisuoaromiaxntaulroegoufe96c.o5mp-o1u0nd0s%.test substance and 0 -- 3.5% Ce, Cr, METHOD: Method: Not noted. Type: Static acute GLP: No Year completed: 1978 Species: Bluegill sunfish (Lepomis machrochirus) Supplier: Not noted. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Not noted Test fish age: Not noted. Length and weight: Average length = 4 cm Pretreatment: Not noted. Average weight = 0.20 g Test conditions: * Dilution water: Carbon filtered well water. Dilution water chemistry: Temperature: 19C Dissolved Oxygen: 9.1 ppm PH: 7.9 CSotnocceknatnradttieosntssodloustiinognrparteep:aOrantcieon: Not noted Stabilityof the test chemical solutions: Not noted Exposure vessels: Tanks containing 16 liters test solution. Number of replicates: one Number of fish per replicate: 20 Loading rate: 0.25 gL Number of concentrations: five plus a blank control Water chemistry during the study: Dissolved oxygen range (24 -- 96 hours): 5.36.9 mg/L (control exposure) 5.1-7.3mg/L (420 mg/L exposure) pH range (24 - 96 hours): 03741 7.9 (control exposure) 7.88.0 (420 mg/L exposure) Test temperature (24 -- 96 hours): 18-19C RESULTS Nominal Element concentrations: Bk value: 96-hour LC50 control, = >420 135, 180, mg/L. 240, 320, and 420 mg/L Element value based on nominal concentrations CONCLUSIONS The test sample 96-hour LCso for bluegill mglL, the highest concentration tested. sunfish was determined to be >420 Submitter: 3M Paul, Minnesota, Company, 55133 Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY fRoellilaobwielidt.y:NKoiiimnifsocrhmartaionnkionng s3.tocTkesotrintegstlascoklsutiinofnorpmraetpiaornatoinontsh.e mAveetrhaogde fish weight is analytical suspect. Sample purity was not properly characterized confirmation of test substance concentrations. and it lacks REFERENCES This study was conducted by the 3M Company, St. Paul, MN, Lab Request number 3844. Environmental Laboratory, OTHER Last changed: 5/24/00 03742 32 Ty * Lo : | ! 4 v1 [.0, 8344 JB TIRES peroopy anne Type Test__Yb- Hout Material Tested FC. -J43 Lod 3 4 test Organism Rlnaepd\ Avg. WeQ.n grams Avg. Size_ Mea Test Solution Volume_ | (o Liters/Tank No. Organisms per dose__ "AG : Tine Started_TB100Pn Date Started_4=H = Date Compleced_d-9-1 Dilution Water Carbon Filter - Well Water (Temp.)3 C., D.0Q,1ppm, PH13 ) Investigator, P. LC 50 (95% Confidence Limits) pg : T E mo Il o Conc, posed. No.Dead, pli ppaplmuall o||No.Dead halei_ppm To ualell ||No.Dead pl ppm ||No,Dead hpH alpeps: \ sol TaoIo boluslo [scl] 20|_ofool] _cJucliall o Pulesll 0Tuleell hile hales ol ao | 0hahallcDafuell olesfsall leolse ) [sao] 20 ol 20 || aJanell 0 halal o o [Jssoolfuudll[Un TJraallssslalll11 halo Jisls) Co rrr rrr 1 | bI oze 1 oe II ye|| wt [wt Comments: J a --- - - J- ee 03743