Document zdMXMmzn4Ze6vvJB1yB53d916

AR R26 -- [367 PROS: A TOX`OINCSIETEYDTLEISNTGTEOMDEERTGEERNMCIENOEFTSHEEVEEFNFSEPCETCSIOEFS OTFHEPLTAENSTTSSUBSTANCE FINAL REPORT WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454-110 Environmental Laboratory Projct Number U2723 U.S. Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines 'OPPTS Number 850.4100 and 850.4225 and `OECD Guidelinefor TestingofChemicals Proposal for Revision of Guideline 208: Terrestrial Non-Target Plant Tests AUTHORS: AnJdorhenwR3.. BProirgcnhole ReyHmeonydL.. KVarnegHeorvePnRDP.AD. STUDY INTTIATION DATE: November 12,2001 STUDY COMPLETION DATE: May 30, 2003 SUBMITTED TO: Envir3onMmeCnotraplorLaatbioornatory St Pa5u3l5,BMuisnhneAsvoeanu5e5106 Wildlife559I8nCtomemrenrcaetDiriovenal, Ltd Eason, Maryand 21601 (410) 822-8600 Page 1of 136 002193 Wadi . PRoCTNO: 4510 OD LASORNSORSTRSCOANETATEAGNS SR TITLE: PFOS: A Tonicity Testto DeterminetheEffectsoftheTestSubstanceOnSeedling So NS i E TUH DYACOb MPTLe EtTITeON.S MAyE20ST,10Om 1m i8RR A SeTn NOS HiPe y tte--s , GoodLaboratoryPractice(ENV/MC/ andC JapHanE MAFMF,( 599 Nob8 San) ,No1 tifi7 cati) onN; o. STUDYDIRECTOR: nSo t sronsonasmassmTaTE I). Boat 0 ey Loz =/2/03 a 002194 Wildlife I somal Ltd PROECTNO: 454110 QUALITYASSURANCE STATEMENT "Thisstudywasexaminedfor compliancewithGoodLaboratoryPractiosStandardsaspublished STa Ne omsieonnaaan,y10A40iTStEX5F9oi.Toaohed1safLgaobefor4985sOWeEiCgDeiFmpcoccmnis ofGoodLaboratoryPractice (ENV/MC/anCdJaHpaEnMM AFF(, 9 59N8ob) San1 ,No7 tif) icat; ion acre DATECODUCTID STUDYDIoRiBeETROsRsoRMEADNTAOGE:MENT Overs `TestSubstancePreparation Nos190h01 Novem12b,2e00r1 Ns November 1129,,2200011 Nev2e0,2s01 Novea1s5,b2e00r1 B Eee HeiandgWehigtht fume Domes Marites yon ArDn r ras 130 Dust Drom0 hyn easmm Norm 1,00 Nevsbct2s0,2 BDO ps0 Sonn N20 nn pm demas Mem Aminve cori ct. . JompdGeen . QualityAssuranceRepresentative 530-03 a 002195 -------------------------------------- Wildife I ional, Ltd. PROIECTNO: 454110 REPORTAPPROVAL SPONSOR: 3MCaporion TITLE: EPFOS:nA oTvofsSseievyeTnSepysetcioeDeseotfPmetiseteEftofte TotSubacs anSig WILDLIFEINTERNATIONAL, LTD, PROTECTNO: 454-110 STUDY DIRECTOR or LEofTrevi Plaksd etSis Mey2003 Viio| n,PR 5 haafas WIILDNLIFTE ERNATLIID.MOANANGEAMENLT: FDeieyn0.oGfAequsaticTo1xDclgyTeeshssad secs -- < ID\EE iofNCeV ihem,nOiD! on Be.S/hefo3 stiofs a 002196 "TOA FCB ONL TENE TS eT A ------ FCreyaoaemdMrtostomainn reero : -- ---- 2 ga tH . 002197 Wildlife International Led "OTM ea0 TAOFBCOLNTEENTS (continued) Tete] SeslingCondonRagSY TABrLEsS err] I ---- DaySeedlingEmergence Test withAlfalfa .........c..oouvvunmmmsmmsssssssssssssmsmsrmsssnisssins 22. Table EBfetcohf FOBSronSeedliTneg Ewmeergeenc,e,Surrviivral,SShhooontWWeeiigghht,waond HeghHtion h21n Tables B Effciof FOSonSeedliE ngEmergencet, Sourvhie ShooShottWeigWhetg,,andmdHHeeighht2na02- 0 TableS Bt So Wet, mites Effects ofPFOSonSeedlingEmergence, Survival, Shoot Weight,andHeightin a21- 2s Tobe EB ffet e ROESmnmSeec liTgEonwh gi,nSgcie,ShootWeight,an Heigha t na2 Table? Ef ft ofo FOS Stii ngEtemiese gnc,Sivataa, ShootWeighSt,hanidHehghtinn21a.Day n Tobe Effca tofPROSSexlionygEgene,sSSruvia,SShootWeigh ndHeghtinn a21.Du y A EE -- I-- IE A 1 Pre] Id 8 APPENDICEEsSered Aopen 2 Chines1015PRL 3) Appin 3 COB AAT rere 32 Appendix 4 ToB e AnnofPEOr S InSoiand SevenSSppeecfieesoofflPatsnfnSSepuoperofofWWiilede 38 FO ---- FE -- 6s 002198 PROJECTNO: 454-110 `T(cOAoFntCiBnOuNedTL)ENETS Appendix 8 TeSRESUNS,LECcr Appendix 10 TestRESUS RYSGESS crn11 Appendix 12 TestResults, Tomato. eres sneseassrseseresssere p-- i } Appendix 13 BUSCHAIACETZANON1. 132 Appendix 14 Results ofWaterandSoilPesticideSETeening vnc 133 002199 a. PROJECT NO: 454-110 SUMMARY WILDLIFE INTERNATIONAL, LTD, PROJECT NO: 454-110 TEST SUBSTANCE: Perflourooctanesulfonatc, Potassium Salt (PFOS) STUDY TITLE: PSeFeOdSl:inAg ETmoxeircgietnycTeeosftSteovDeetneSrpmeicnieestohfePElfafencttssofthe Test Substance on GUIDELINES: ~~ O20E8;CDTeGrrueisdterliialneNofonr-TTaesrtgientgoPlfaCnhteTmeisctasls, ProposalforRevisionofGuideline `OOPPPPTTSS 885500..44120205 ((PPuubblliicc DDrraaff})) NOMINAL TEST LEVELS: 0 (Control) 3.91, 15.6, 62.5, 250, 1000 mg a.ikg dry soil TESTDATES: SExTpUerDiYmeIntNaIlTSItAarTtIO(NO:ECD): ExperimentalStart (EPA): ESxTpUerDiYmeCntOaMlPTLerEmTiInaOtNio:n: N`Noovveemmbbeerr 1122,,22000011 November 12,2001 AMuagyus3t0,2280,023002 LENGTH OF TEST: EExmteerngdeendceGrPoorwttihonP:or2t1ond:ayusp to 205 days (va:ries by species) TESTSPECIES: sAaltfiavlaf)a, (OMneidoinca(gAlolsiautmicveap,a)F,laRxye(gLriansusm(uLsoiltiautmispseirmeunm)n,e)L,etStouycbee(aLnoc(tGuleyacine max), Tomato (Lycopersicon esculentum) RESULTS: Common name(Latin ame) Sposes Fumi EsCeFnewiv(nega Endpoint TRoymeagtaos(y(cootperspiecroenmces)clenum) ROniiosn ((Aedlimccageop)save) SFoyabesan(Lima(mGhueinsemiarn) SGorlaumnicneesaee LLeiguemisnone Lincpeismeinose 71152715 SSShhhooooootttWWWeeeiiiggghhhttt 5a3s SShhoooott WWeeiigghht 6 Shoot We -8- 0uzzo0 --_--_--,-------- W, PROJECT NO: 454-110 INTRODUCTION This seedling emergence study was conducted for 3M Corporation at the Wildife Intemational, Lud. greenhouse facility in Easton, Maryland. The in-life portion of the test was conducted from November 12, 2001 to June 5, 2002. Raw data generated at Wildife International, Lid, the study protocol, and a copyofthe final report were filed in the archives locaed on the Wildife International, Ld. ste. Keypersonnelinvolvedinthestudaye listedinAppendix 1. PURPOSE "The purposeofthe studywas odetermine the effectsof Perflowrooctanesulfonate, Potassium Sat (PFOS) onth seedling emergence and growthofseven speciesofplants. EXPERIMENTAL DESIGN "Thereweretwopartstothisstudy.Thefirstpatwas a twenty-onedayseedlingemergencetest "Thesccond par, an extendedgrowthperiod, followed immediately. The experimental design for the overall study consistedof a negative control and five treatment groups, with four replicate pots in eachgroup.Tobeginthetwenty-onedayportion ofthetettenseedswereplantedin achpot. Test concentrationsof PFOS were made by soil incorporation {0 each treatment group prior tothe planing ofseeds. The nominal test substance concentrations were 3.91, 15.6, 62.5, 250, and 1000 mg of FOSperkilogramofdrysoil (mg 8./kg).Theserateswereselectedbasedontheresultsofanon `GLP rangefinding test, and are not necessarily indicativeofexpected environmental concentrations. A contol group, which recived no test substance incorporation, was maintained concurrently. Seeds were impartially assigned to growth pots on the dayoftest initiation. The replicate pots were placed in a randomized block design on a greenhouse table afte planting. Observations of emergence weremadeon Days 7, 15, and 21. Onday21, observationsofhight, and assignmeonft plant condition scores were made. Fresh weights were conducted on al replicates containing more than one living seedling. Where possible, one seedling from cach replicate was then left to grow until plants produced frit Gi, the extended growth period), whereupon, plants and fruit were clipped and weighed independently. "9 002201 ------------------------------------------------------------------------ PROJECT NO 454-110 MATERIALS AND METHODS "The sndy was conducted secordtoitnhge procedures outlined in the protocol, "PFOS: AToxicity "TesttoDeterminetheEffectsoftheTest Substance onSeedling EmergenceofSevenSpeciesof Plants." Changes otheapprovedprotocol ar listedin Appendix2. Themetusehdinocondductsing this study were based upon procedures specified in the OECD Proposal for Revision of Guideline 208: Temestrial Non-Target Plant Tests (1) and the USS. Environmental Protection Agency Series 850- Ecological Effects Test Guidelines OPPTS Numbers 850.4100 (2) and 850.4225 (3). TestSubstance `The testsubstancewasreceivedfrom 3MCorporationonOctober29, 1998,andwasassigned `Wildlife International, Ltd. identification number 4675. The test substance was a white powder identified as FC-95;lot number217; wia retanalhysis da of Augu3s1t, 2006. Information provided bytheSponsor indicated tetsubstance purityof86.9%. Thetestsubwsastsoraednatamcbieent room temperature. PrIepanraticon aondSroilporaoftTesitSubostannce `The test sol was prepared by mixing PFOS into bulk test soil with a measured soil maisture of 15%. Test substancefor treatment groups 3.91, 156, 62.5, 250, and 1000mga.i/kgwaspreparbedy weighing five known weights (0.2690, 1.1, 43, 17.1, and 68.5 g) of PFOS. Approximately 1000 wasremovedfrom a measured70kg ofbulksoilandmixedwithweighedtet substance orcachtest concentration. Theremaining 69kg ofblk salwasplaced ito soil mixes,an the 1 kg ofsoil with estsubstwaasnadcdeed. The constituentswerethenmixedfortenminutesin ordertopreparethe test soil for each treatment group. Soils were mixed from lowest to highest concentration to avoid crosscontamination. The negative control soil waspreparedprior to thetreatment groups,butin the same manner, xcept no est substance was added. ALth completionofmixing, the tet soils were sampledtoprovide materialforanalytical confirmationofthetest concentrations. Analytical samples were stored at ambient room conditions for up to four days after their collection until they were processedfo analysis. TestSpecies "Thecommonandscientificnamesforthesevenspesistested,theseedsourse, andtheir approximate plantingdepths relisted below: eo 002202 ------------------------------------------------ ne PROJECTNO: 454-110 TFNesIitM(SsLp(ieHcneinduecsarg/aVasrrasiveeit)ym:NaNoonne)eGiGivveenn FArSooewmeehdreNSdoauMricle,P:rRodeurcetosd,N,oTrXw.ayU,SAA,USA PlantiSSnmmgDepth RLryiceengrt(sulojciesmpev(rceT)nLno/eeS)cMsupmGahmae)nhrcotaiamen3 TTMeeeyorerroSdSSeCeeodd,CCBoo,CCoolttaaggeenMGGDor,vovUe,e,,SOOKRR,LUUSSAA 1p6mymm STooymbaen (Gchocpienerimoasn)esGcrueleenntEav)yResJMoehyoeeryS'seSedlCecot,edBSead,lAlmbMiDons,,MUES,,AUSA 2mmmm These species were chosen because they represent ecologically important fails, and are readily cultivated test organisms tht ae widely usedinresearch. An additional consideration was0 select specieswhichcould be grown to fruiting relatively quickly, while maintaining a reasonable plant ize, Seedswereselectedfrom asinglesizeclasswithincachspecis inoder 0reducethepotentialfor bias from differing seed sizes. Seeds used in ths studywerenot treated with fungicides, insecticides or repellents prio to test initiation. TestSoil "The soil used for the tet represented a loam soil, and was composedofkaolinite clay, industrial quartz sand, and peat mixed in a 2:25: ratio (s:ow). Crushed limestone was added to buffer the pH ofthe soil, and a slow-release fertilizer was addedtoprovidenutrient essential for plant grow. A sample of soil representativeof that used in this study was sent to Agvise Laboratories, Inc, in Northwood, North Dako, fo analysisofthe particle size distribution and organic matter content of the soil (Appendix 13). Th soil was determined to consis of 49% sand, 30% silt, nd 21% clay, withan organic matter content of 2.1%. Thesoil pH wasmeasurebdy Wildlife Intemational Li. to be 7.79. A copyofthe complete report from Agvise Laboratories, Inc. was filed in the archives at Wildlife International, Lid. along with the aw datafo this study. BlanotfSienedgs Seeds were planted in plastic pots (approximately 16 cm in diameter and 12 cm deep) ontheday of test substance application. A template was usedtogently compact th soil andleaveten uniform holes for planting. One indiscriminately selected sced was then planed in each hol, or total often seeds in each pot. Personnel thenclosedthe hoes by slightly depressing the soil surface with their fingers. 002203 ue ---------------------------------------- Ww, PROJECTNO: 454-110 `WatoefSreeidlninggs `Water lost through transpiration and evaporation was replaced by subirigation with well water from the greenhouse facility. Seedlingsweresubirrigated as needed during the test o minimize the potentialforthe leachingofthe test substance through the soil. Subirigation trays were filled to predetermineddepthtohelpstandardizethe amountofwaterdeliveredtocach tray. Thedayson Which watering occurred are listed in Append 5. `Environmental Conditions `The environmental conditions (light intensity, temperature, and relative humidity) during the test aresummainrApipezndeixd5. Thetemperaturewithinthegreenhousewas controlledwith a Wadsworth MicroStep S/A Environmental Control System. Atificial lighting was used to supplement natural sunlight in order to provide a uniform 14-hour photoperiod. The light intensity, temperature,andrelativehumiditywithinthegreenhousewere continuouslymonitoredduringthe test witha Campbell CR-10 datalogger. esticideandMetal Screeningof WellWater andSoil "The well water and sol used for plant studies are analyzed periodically for pesticide and metals. Noanalytesweremeasuredatlevelsthatwere expectedtohaveanimpactonthestudy(Appendix 14). Reports forthe latest analyses are stored in the archives at the Wildlife Intemational, Ltd. ste in Easton, Maryland. `ObsersnvdaMetasiuroemnensts Observations on Days 7 and 15 were conducted to document scedling emergence. Observations onDay21weremade todocumentseedlingemergenceandgrowth,and todeterminetheconditionof individual seedlings. Observations consisted ofnoting whether emergence had or had not occurred, and assessing the conditionofeach seedling. Emergence was defineda the presenceofvisibe plant tissue at the surfaceofthe soi. Seedling condition was described by noting the presence or absence of possible signs of phytotoxicty such as necrosis, leaf wrinkle, chlorosis, plant lodging or plant stunting. Each emerged seedling was then assigned a numerical score (see Table 1) that described the plant condition (4). Condition score is a subjective or qualitative assessment that determines whether damage is slight, moderate, or severe. A score of 10 doesnotmean that 10%ofth plant is showing the effect (e. ehlorosi), merely that the severity ofthe effec (e.g. chlorosis s very light. 12- 002204 _-- PROJECT NO: 454-110 Onday21, seedling height was measured tothenearest whole centimeter from the surfaceofthe s(osiolytboeatnheatnidpotfotmahteo)t.alleAstllelaifvi(naglfaslefea,dlfilnagxs, lettuce, except onion, and ryegrass) or to the apical meristem one per replicate (when available) were then clipped at soil level, combined, and weighed within 5 minutesof clipping. The total weightofthe shoots plant. was The divided by one plantp the number erreplicate, of seedlings weighed when availble, which inordertocalculatethe mean weight per ws notsacrificedwas allowed t grow for participation in the extended growth tes. Alflf, lx, soybean, and tomato were grown uni fit production. Onionsweregrownuntilan enlbualbr wasgeviedendt. Let wat sgruowc nune til `adequateleaftissue was available for analysis. not observed. Rycgrass seed production was anticipated, but was. SSoilamplandiAnanlysgis On the dayoftest sol preparation (November 12, 2001), thre sol samples wer collcted fom esch treatment group fo verify th test concentrations and determine the homogeneity ofte test subisntthe caarnirc(oie). Onesamwaspcolllcteedfomthecontrolgroup. D0asampyles werecollectedfromthe soilfromeatectgrhoup renaftaerpnotsiwernefilgledforplanting. On day21andat terminationof te ast test species, two soil sampleswer collectedfromthe treatment 70ups to verify and determine homogenciyofthe test substance in the soil. One sample was collected from the control group on both sampling dates. These sampleswerecollected rom test pots.Day21sampleswerecallestdbyremovingasmalpotion ofsoilfromthe surficeandplacing itnthesample container, Testtermination samples werecalectedon Jun5e, 2002using a oil core sampler which removed a cylindrical coeofthe soil from the surface to near the bottomofthe est Pot. Since the fest was terminated on different daysfo the various specie, the tes termination soil samples were collected from the pots containing ryegrass, which was the last specs to terminate. Samples were stored at ambient room conditions until analysis was begun on August 14, 2002. Chemical analysis of the soil used in this study was performed by Wilde Intemational, Lid (Appendix 4).The tst substancewasusedtoprepare calibration standards. TiSssaue mplanidAnnalygsis OnDay 21, December 3, 2001, following observationsofemergence, andmeasurements of eight andfresh weight,threesamplesofplanttissue weretakenfiom cachspeciesatcach test concentration, and analyzed for PROS. The three samples were obtained from compositeof issue ie 002205 --_-- Wdite Led PROJECTNO: 454-110 from eachofthre replicate pots. Three additional plan tissue samples were taken from the single plant remaining in ach replicate, when avaiable, for cach specs at each tet concentration at test teromfthieexntenadedtgroiwthoperinod. Plantsfom thefourthreplicateineach group,when present,were usedtodeterminethemoisturecontent ofplanttissue attheendofeachportionofthe test (21-day and extended growth). Samples were stored frozen until analysis. Chemical analysoifs the plant issue collected in this study was performed by Wildlife International, Lid. (Appendix 4). The test substance was used to prepare calibration standards. Dates of test termination for cach specis are listed below: Spits Alita Fix Lemce Onion Rycprss Sopbean Tomsto DTateeorfmTeisnation Aprit2, 2002 February 14,2002 Jamary 18,2002 Jamary 18,2002 June 5, 2002 Samay 18,2002 February 14,2002 Data Analyses Statistical analyses were used 1 aid in th evaluation of effcts of test substance application on seedling emergence, survival, shoo weight, nd height. These variables were definedforstatistical analysis as follows: `SeedlingEmergence:Thenumberofemergedseedlings ertnplantedseedsincachpot. Survival: The number ofemergedseedlingsin achpotthatwerelvingattet terminationper ten planted seeds. ShootWeightThemeanshoot weightofsacrificedlivingemergedseedlingsin each po. Heght The average hightof vingemerged seedlings ineachpt. Test data were evaluated to determine theno-observed-efectconcentration (NOEC) and lowestbservable-offect-concentation (LOEC) for condition and growth. The NOEC is defined as the `maximum test subsiance concentration that shows no adverse phytotoxic effects and below which no phytotoxic effects are manifested. The LOEC is defined as the lowes test substance concentration used in the study that shows an adverse effct on a variable of interest. Mean seedling emergence, m 002206 w: Lid PROJECTNO: 454-110 survival, weight, and heightof the controlandtreatmentgroupswere compared with Dunnett's Lest using the DUNNETT optionofthe GLM (general linear model) procedureofSAS version 8 (5). Significance was determined at the level of 0.05 (7<0.05). Dunnett test was used to aid in establishing the NOEC by determining which treatment groups differed significantly from the control soup. Statistical analyses for species also included the determinationofeffectrates(ECestimates) and their confidence limits using the non-linear regression analysisofBruce and Verseeg (6) when reductionsintestendpoints amongoneormoretreatmentgroupswere25% ormorerelativeto control means. AnalyseswereconductedusingtheNLINprocedureofSASversion 8 (5). EC, values (i.e. ECys and ECs) were defined as the test substance application rates that caused an x% change in the treatment group mean emergence, dry weight, or height relative to the control group. EC, estimates were calculated using nominal test concentrations and reatment group mean values with the folowing equation: . | Ro O{(log(ECHog(C))o +2] C0 wB=here Lk theprotic biteresponsesconcentrationC c=0 Rh) = hecurreadtiidveirtsrespeornshefsoarncdorl,eora isin 1og(EC,) =the logarithmof thepredicted ER givingan xpercentageof decreaseinthebiologicalparametervs. the 5Zh sihsoaandmdeveiviasnonoef hwehiocrhmpedrseunnieon he ofbistd rssation ck Effcctson survival were designated gs LCs values, and were calculated using the method described above. Ifthe itofthedat totheregressionmodelwaspoor,or ifconfidenceintervalswerenot calculated, theEC,estiwemreacatlcuelatsed using linea interpolation (7). AnslytiealChemistry RESULTS Artificial Soil. Samplesofartificial soil collected on Days 0 and 21, and at test termination `were submitted and analyzed for PFOS. All negative control artificial soil samples were <LOQ for PFOS. Da0y analysesofthe 3.91, 15.6, 62.5, 250, and 1000 mg a.i./kg treatment levels had mean measuredvaluesof 3.61, 11.1, 50.8, 276,and 998mga.4/kg, correspotno9d2i.3n%g, 71.2%, 81.3%, 110%, and 99.8% of the nominal concentrations, respectively (Appendix 4.19). PFOS measured 15. 002207 Wildl ki Lid PROJECTNO.: 454-110 values for test termination samples are presented in Appendix 420. The 3.91, 15.6, 62.5, 250 and 1000 mg a.i/kg treatment levels had mean measured values of 129, 3.56, 162, 157 and 474 mg 8.i.kg, comesponding to 33.0%, 22.8%, 25.9%, 62.8% and 47.4%ofthe nominal concentrations, respectivelyattesttermination. A representative ion chromatogramofanartificial soltestsample is presented in Appendix 421. DSaya2m1ples.Theresultsofsoil andtissueanalysesofsamplescollectedonDay21 were not considered represcatativeofthe actual levelsof PFOS in soil and tissue, and are therefore not reported. The measured levels of PFOS in soil samples were generally well above nominal. The apparentlyhighconcentwerreathtouighottnobse. an artifuc resultingfromtwofactors First,the processof subiigationduringthe testwas thotoucongcenhtratte PFOS nearth soil surfaceoftest pots. Second,thesampleswerecollectedby removing asmallamountofsoilfromthetopofthesoil profile. In addition, issue samples collected on Day 21 were thought to have been inadvertently `contaminated during collection. Due tothesmall size ofthe seedlings on Day21,and the apparently high levels of PFOS at the soil surface, it was determined that soil particles were attached to the seedlings and biased the analytical results. PlantTissues. Samples of fut tisue fromfivespeciesofplant alfalfs, fax, onion,soybean, and tomato) grown in negative control and PFOS-reated anifical soils were collected at test termination (fo a given species) and analyzedfor PFOS residues. Samplesofvegetative tissue fom seven speciesofplant (alfalfa, flax, lettuce, onion, ryegrass, soybean, and tomato) grown in negative control and PFOS-treated artifical soils were collected at experimental termination (for 8 given species) and analyzed for FOS residucs. Measured PFOS concentrations are presented in Appendices 422 ~ 433. Representative ion chromatograms of plant fut and vegetative tissue test samples are presented in Appendices 4.34 and 4.35, respectively. 002208 -16- --_-- Wide Lid PROJECTNO: 454-110 `Theresuofltitssuse analysesare summarizbeyd species inthe following tables. Alfalfa Totaldaysof exposur=e 141 I sa -- -- 0 EE -- ] YS-- e we e oa ] ] m C e Vw oT m ae Noweioig || Flax Totaldaysofexposu=r4e cmogwal) Ve9isnT wn Ei] ] Ca se pp on Cas 51 PS 3 --] C -- -- SI O Lettuce ~Totaldaysofexposu=r6e7 emga l m I-- e --s-- n cmTGs Semsiate | esse T i &T T NNooumgiat || C 1 Nw oocing |Notmppiabie Onion --Toial daysofexposure = 67 "NComoimScalenatn Conor I---- -- EE -- se--T --a --n 1 -- Co m w NoeT wslhvwinngg| | NNooewhliwvnigg J Rycgrass - Total daysofexposure = 205 Coma ma3s Tos wi a| Cs S -- s -- n S-- -- T NTewT gat-- e C -- m ----T ----& -- T -- Nop-- e | CVe owels1Nougpieate J ae 002209 ocr0s tito Soe Toldsfase67 "NoSom il Cioncn enta ratilon [ Commol---- 1 <0|<0i8 | 3 Te ie use 13% om | es Te 2 as Th | o 33 | I CW | Noneliving| WNoneliving | mgalts | Vegemtion | Fit | Cows 1 6 1 <a | se I &@s 250 Cho 131 --------1 Ts | o oss | insufficient ample | Insufficientsample | 1 Nomeliving| Noncliving | T a Ra r r e me otRs eus BiologicalResults ST TYRC [7 Fee Twp fmee peer ree e [__2ldwyEmegenceI ZidaySuviva | foerTe "Monocots: ea e w mma nw -- Trn-- q mme nom m ww mwwsn a mw e -- in | | _| i. 002210 - % , OO romero: sso [7 m pe y we Monocots: T-- it-- s -- e A a im p e esm fe egaa re i sg ] | mem a a m|oahwm mwememm as we s ue manna me ceorimeoiosdy tseas`eCONoiCLpUotSIiONS cons nn -- us Pe ESEErtamm, coomm EEee A i e ffia vn otpcs en = Z_T om JBE o || E|aEnee C| E EE bin `Therewerenoadditionalfindings ofphytotoxicityatthe terminationoftheextendedgrowthportion 002211 _-- PROJECTNO.: 454-110 REFERENCES | POrEopCoDsalGfuoirdeRleivniesifoonor fTGeusidnlginoef 2C0h8e:miTcearlrse.sri1l9N98o. nGTuairdgeeltiPnaenftorTeTsetsst.inOg orfgChaemnicaoles Economic Cooperation Development 2 GUuSi.delEinnvesir(ornmaefn,taOlPPPrToSteNcutimobnerAg8e5n0c.y41.00: 199T6e.resSvealriPla8n5t0.ToErcoilio,giTalieEf1fe(cStesedTiensgt Emergence) 3 US. Enviroumental Protection Agency. 1996. Series 50 Ecological Effects Test Guidelines (draff), OPPTS Number 850.4225: Terrestrial Plant Toxicity, Tier II (Seedling Emergence). 4 MMFreeaatnsshu,roeRdimosenbneWtretaendEd.SAcaninaelndcyesR.iosnSoaofludtPhlEea.rnTWRaeelsebpedorntSs.eecsn.1c9e7P7Sa.goeusDtes1,i3gA3nu3boifunmFBe.UlndiTvreuErxcspoietwyre,i,mAeelnds,tbsaRremasane:dartchhe 5 SAS Institute, Inc. 1999. SAS Proprietary Software Version 8 Cary, NC, SAS Institute, Inc. 6 CBornutcien,soRuosbeTroxticDi.tyaDnadtsD.oEnnavlidroJn.mVeenrtsatleeTgo.ico19o5g2.y anAdSCahiesmiicoarly.P1roce1d4u5r5e14fo5r4.Modeling 7 `INnAohgriebbnicetyri,ognEKnivCniogrn,ocnenmTteJrn.attailo1Rn9e9s3(e.IaCr)cAhALLpiapnbreooarraecrIhnst,e(rVDpeourlsatito,inonM2.iM0ne)ctshooUdtSsf,orESnuvbilreonhmaelntTaolsiPcryo:tectTihoen 5 Wide Internationa, Ltd. 2003. Proje No. 454C-120, Van Hoven,. L., MacGregor, PJ.eAr.f,uaonrdvoNcitxaonnd,suWl.foBn.teF,iPnoatlasRespioornt.SAlnal(y9tFicOa)l MietAhrogdicVialliSdoationfor the Determination of 5 JWAi,daend INnitxeornn,atWi.onaBl., LFtidna.l R2e0p0o1r.t.PAronjaebcitcNaol. M4e5t4hC-o1d25V,aliVdaadnoHnoovrent,he.DeLs,erMmaicsGetrieognoro,f Perfluorooctanesulfonate, Potassium Salt (PFOS) in Plant Tissues. 0 0 02212 _-- Ww, Table 1 Seedling Condition Rating System PROJECTNO.: 454-110 Rating 0 Cotepory No Effect Description No noticeable effect J TT Effect barely noticeable 2 Slight Effect `Some effect, not apparently detrimental 30 dEfeftercitmemnotarle pronounced, not obviously 0 "Eref covermof derate,e plants c sppeart able0 50 Moderate Effect dMoourbetflualstingeffec,recoverysomewhat 6 Lasting effect, recovery doubtful 70 T Ficavy injury, lossofindividual leaves 80 Severe Effect Pllaeanvtesnearlydestrafoewysuervidvi,ng % Occasional surviving leaves 100 Complete Effect Deathofentire plant RatingsalFerosafdnPaslp,anRetdRreoospmo:nsbeasn.deRPoangearsld15.t.T2ailbn.eBr.t.Tr1u9l7o.veD,esai.gnReosfcFsicelhdEMxepterhiomdeinsntsWeaenddtShceienMceea.suSreomuetnetranWdeAdn.alysis ScienceSocey,AuburnUnivers, Alba. 1- 002213 _-- w, L PROJECTNO:: 454-110 Tabl2e EffectsofPFOSonSeedlingEmer`genEcem,SuerTveirvsatlwg,iSthehoAoLtnFWAeciLgFheAt., and Heiinag21h-Dtay Seedling CNonceenTtreaationoEmgd%RSeducetion)ling (mgaike Dal Day15 Duyzl SSeurvS liveal koveg H(eem)nn (Recto) (kReducion) (%Reduction) (MeniSD) (MensSD) (MensSD) (MemsSD) (MemsSD) (MeansSD) Cowl 8754150 S004LIS SMliS 875209 0125008 654064 391 8255050 8504)038 80)05 252)080 0U2L030N 156 [54i1]50 B0aSLA BaMnL B0S)LA 0a0ss002 5 Taanlhs S0[0esLyIS Sealls BoOnSS 010ai2s0010 J 2a0)1 Ia9e) A5l%)N 6a2m54)170 0D[s03008 1000 250[a42890 252040289 3(254)330 10S01) T 00G2300)08 TT MeTarnca+imSen-tgTrroeuaptmmeenatngrogupmeeaan llusorfmrineutsFoonemstaendcaorndtdevmiseionn(.Dunne TRpO) 64a2)075 5465%0)76 614)09 28om0)50 10(450%0)00 002214 -2- _-- Wad Lid PROJECTNO: 454-110 Table3 Effectsof PFOS on Seedling Emerge`nEcmeer,gSeunrcveivTaels,tSwhiotohtFWLeiAgXht, and Height in a 21-Day Seedling TN hCo(nmcegeanirke aei)on o Day? E0%De Raedyu1c5t.ione ) a Duy S (SSuerlvlie veiamlh l oShwiei no n onHe(aeig mi) on (den3SD) (MeansSD) (MensSD) (MemiSD) (Mem SD) (Means Conwol 7252126 7258126 1256126 7256126 0194009 894027 391 60(12)00 T00%s)L4l 725)210 67%54)126 01824)002 156 B0104%0)8 8010:%0)8 800%0)08 Bc0l0on4)0R OaI0s0)0B @5 0":r1m0) 0Cs1m00 8m01y00 80e4m100 01a62s0017 J AB)IN IB)N BA)IN 22552%1)00 00Os1050)09 1000 [0= [o= C or C)o NA TT N/TAecaNmoetnaptpgloicwablmeesainncsetShierpnwicearntnyosfuerrveinvtiFnopsanescaomnesosueemennt( . Dup0e5) Mean + SD Treatmentgroupmean losorminson sandarddevaion 87a4%0)50 88a1080 8224)084 13(48%0)35 Na - 002215 2 _-- PROJECT NO.: 454-110 Tabled EffectsofPFOS on Seedling EmerEgmeenrcgee,nScuervTievsatl,wiSthhoLotEWTeTiUghCtE, and Height in a 21-Day Seedling. TCNRoneceEnwtraetioon tEegSedi0%nRegducion) (menifg) (MeDmayi?SD) (DMaemyssSD) SuSrveilvael SeeWes R(enm) (MDeemya2SlD) ((kMReedwuncitSiDon)) ((kMReedmuncstSiDo)n) (e0%aRednuctSiDo)n) Commi 8758080 8755050 8752050 8752050 03840080 642051 39 M@i%m SM0:0m S>WA0m S@N%L0E 0a2s0)0S 156 875)210 90@04%115 SM:0lIs S@0s%llS 00as0)8 05 85002%1)29 85o0%4)129 SNae)129 ES2:)12 00[553001 20 Wal)e B%l) 8252)126 67@ 55096 0015.%0)006 1000 L2252)1260 12502%1)36 12654%1)260 05o0u40)88 00[02020000 TTTrcuiMmeaen +nStgTrroeusptmmeenatnsgrouspgmfecaannptlysGofrmeinnsfoonmsBtaencdaornddevmienn.(DuVno ep00). 50@80%56 S0asni)om 26(529%0)4 10a2)006 10w40y000 2- 002216 ~ Waa Lid -- PROJECTNO: 454-110 Tables Effectsof PFOS on Seedling Emerg`eEnmceer,geSnucreviTveaslt, wSihtohotOWNeIiOghNt, and Height ina 21-Day Seedling THConceentration (mgaifg) Newe0%oREedeucetSioen len SuSrveivaml Day] Days Dy2l | (iReduction) Sewane(eem (O4Redoction) (Reduction) (ensSD) (MensSD) (MemsSD) (MemsSD) (Mem iSD) (viewsSD) Conrol B0020 80:12 BTS1S0 BO4129 01040006 812085 I B(I20)B Saolna 9250205 90004)LIS 00a94s0)015 156 75a2030 8205%505 8254)050 8M:)0 00f7iy006 @5 S0a0wa2)00 6a2s52)26 62255)261 3255)0500 0a02m40)019 3 2a0s0.)24 4030%4)36 40(0sse3)i6r o2) a 1000 ao 0 [o0 coom) aaooo) a TT N/ATreNmootnatpplriocaublmeesainncetshgeneiwceuentay isrfivnifgoplmattseacmoenavsoureemnent(. DueTRp005) Mean+SD Trestmentgroupmean phasorminus one standarddeviation. 73%2045 73io4%0)1 266%0)8 fad he 002217 .25- _--m PROJECT NO: 454-110 Table 6 Effectsof PFOS on Seedling Eme`rEgmeenrcgee,ncSeurTveisvtalw,itShhoRoYtEWGeiRgAhtS,Sand Height in a 21-Day Seedling CCoe ncentration Nebe%dReEdmucdtiSone) dng (mgaike) (MenDasyS7D) D(aMnys1S5D) (MDeaayn2SlD) Col 775409 8004082 80508 SuSrevilvialm ((RMeedmucstSiDo)n) R00S0R SeaWeg ((MReedwucstSioDn)) 01250035 R(eegmn ((M%ReedwuctSiDon)) 1694069 39 156 5 J 1000 TT BS1O0S%L)00 92564%0)8 92C5e4n09 92c5l5o09 OaM4z00B T0%1)91 910%:0)8 SWE 9eNsw0R 00a79s)00M 15a0):088 25@4%09 850401)20 8"250:09% 000B2L%0)0M* A02l)ar SaTw)i s7a5sa1h26 Saa0m 00OL1s50)06 a00) 02o55m0500 07512%0)50 07512%0)30 00a1s00)100 MeTarnes+mSeDn.tgTrreoswtmmeentangrwouap gmeeannptulsyoGmineusnonerostmanhdearcdodnevtismoena.n (DU5RE PB) IS4)s128 11B97%e)0T 13I 62052 37a4s0)97 200 40000 002218 -26- _--_-- Wodite I Lied PROJECTNO: 454-110 Table? EffectsofPFOS on Seedling Eme`rEgmeenrcgee,ncSeurTveisvtalw,iSthhoSotOWYeBiEgAh,Nand Height in a 21-Day Seedling. TEConceEntration nih) Newe0o% Rbedmuectaiodn)sedg Dwr Day15. Duy SSuevliig (ihc) MemsSD) (MensSD) (MemaSD) (Means SD) SeWegT C_i(RMcehmeoSiDm)) H(eme) (GeumRnedsmion Conmol 9755050 1000000 10004000 10001000 3634058 283417 391 86 25 0 1000 TT 978)2050 97a54050 975o1%0)30 97a54050 364s)02%6 95a0)058 950%4)038 900358 950%5)058 36323)030 I0@00%:000 10004000 1000%4)000 1000)5000 40c0i4o0)32 10@00%5000 1000)4000 100@0)1000 975o4%0)50 207w40n3100 9752%)050 1000)4000 1000)4000 1000)5000 0(57w2a0)047 `MTeeacnamSeDng-TrroeusptmmeeanntgrosugpnmTeiananptlyusdiofrmfriennutsoonmeshaendcardodemvnieaatinotn(,Dour l p00) 2684317 26%94)331 30f7e22)51 25a420260 41s2v0)3* 002219 27- ------------------------------------------ PRONJO.E: 45C4-1T10 Table EffectsofPFOS onSeedling Emergence,Survival, ShootWeight,andHeightin a21-DaySeedling EmerTesgtwiethTnOMcATeO ThN Coencemnratm ion ba 0% Redo acson) teSSuS revlivlael eSked Wegn o(Hcg emg)n (mgaike) (MeDnasySD) D(aMyem1s5SD) (MDeamys2SlD) ((MMReefmuacStiDo)n) (%(RMeedumcatSiDo)n) ((%MReedwusctSiDo)n) Comrol 6502191 S00m0m 925409 925205 03540M3 528075 39 6504)17 B0aSL)IS 82a5i41)50 82[524]150 04c02l0e0)81 56"2TM0)31 156 $2[22]29 R221)7 25ai4s1)7 GM[Eely4 0240108 (15%) 482084 2) @S sNsew1)91 BAI)T S0I0)D 62a5m5)09 0a0)m 26(5500%1)4 0 12s1a%l)sr 72a594)09 72522)096 075)209 OoA1N%)AS 20(102%0)00 1000 1000% oms21%3)00 s9i% a ooor a fl TTTecauNm/A ~eNontapptligcraobulpesmiencnetsheveigwneTrcaenntolysurveirveinntplraonmtshsemceosnstrrelmeenatn. (DUS 5, 009) Mean +SD Treatmentgroupmeanpas orminusanestandarddevidion. 002220 .28- _--PROJECTNO.: 454-110 Tabl9e `Observed NOECan`dWCeailgchutl,aatneddHEeCixghEtsinta i21m-fDoaarytPSFeeOesdSloinngSEemeedrlginegncEemeTregsetnce, Survival, Shoot See NEOdECm) ws Alf aEsme)rgence @SiHl @H9e Bmw CDovLe sUsFearcL wih EECCya M3s2 saaml o>is iLCes 2az31m i EECey w M2 e2s " Seon Reps vBowEwQ)m wen @Emsepoce EECCye S@w9im 110p HOsehp EECeh @We%n EECee Bwe 3213 TM60 Fax asEone)gmce BECao 339 a16 " oWeSg)t EEae Soe aEomne)yee CECyw @Swv9inl Leow m18 Iden EmS] aS0i)l 1CCye @Ho9gr EECCey mM6 00613 1nwm @We%pn EEaCo 181s6 4m6d0 i1ns lemce aEsnepmce BECCow s3s a3M0 siosn @Su9l LLeCes 2m9 ewam P-a @He9p BECas @We9ge EEey Tomo aE0m)egwe EECey 5Se6id iLocye wHaa)g EECLs B6s9 d9w9 duoe aMseet EEChs W0e EECC,o 2sm 1sam ms1a aWesgi EEcCos Onion @Emsegmee EECes m2w8 a12s2 e19w5 - aSasal wLepe m13 o1ss 2i1n00 aHe5p EECCye 4251 ao4o 2sae 6Wsep)n EECwh m1i9 0is9 92%10 TTConfidence ervals coudrorbedeen. Bume CDwL gUpceL (eget MM3e 2BI 4s 014 m 0 wam 4m13 w4s8 sm 25581 9999 i10w 010000 - :dGMiDo - . Mm e mWm e4ss m 10 m1mse o JI eM8 adest 6ws7 4m5s0 ioes @2s1 adsn aimm :1s7 a 9ssman -29- 002221 PROJECT NO 454-110 Appendix 1 Personnel Involved Inthe Study `Thefollowingkeypersonnelwereinvolvedin theconductormanagoeftmhiesstnudty: (1) Henry O. Krueger, Ph.D, DireofcAtquoatric Toxicology/Teresrial Plants and Insects 2) John R. Porch, SupervisorofTerrestrial PlantandInsect Studs (3) AndseJw. Brigaole, Biologist (@) Raymond L. Van Hoven, PhD. Scientist (5) Willard B. Nixon, Ph.D. DircctorofChemistry 602222 20- - Woildisfe InternationalLed POEMBA Appendi2x Chatno Pgroe tocsol Ths dywas conducted scoring he spre profs ithe followingchanges |The RperototcolvaessasmnenneedaeidsetodpBcoiluwedeppcrromondeosf hreoergeasrd.ingndday2fl1sdpteandcsoilslipnl5 3: ec Taatoesn mo posigsh dTasyo,nfosoalnzftedoqFiua5rt5s saaedn,,aScnOdEp,ettmain t d2ns1o5y. 4 morsree wecri. ObservationsofemergenceweremadeonDay 15ratherthan14. 002223 ie ----------------------------------------------------" PROJECTNO: 454-110 Appendi3x CertioffiAncaalytsies INTERIM CERTIFOIFACNAALYTSIES `Centre AnalytiScaMLPraRoedvbuiscoito:nrP1RCa(O3O/St7,A/L0Ro0oe)ftre2ri1e7neces: 073-0184 ome TT Referein.c8:e6S9D%-018 Cee Tw) eII.eCoalmn [ e TIm AoE am aC ahL ET]E 3 5 NPwoonar? & im RrSapealtta asrimpeetit] i ELE 5 an [Toml%lmpurity NMR)[7 TT] L1 oOosRwmEx r 3 rLohd ween) 76 Sooosomamanat|| 153 wut B Er T o ree e Frey ELE Eh 033ws ! [Res Sohvets QTGA)[22 | owe Dens TaTeameaw [ [L TE EiEET] ame i32 FhBriieddee EeirtweiaasrsmeE egRaea aE]nL11 SD owie0 mn ~ 55 NSu~ mea: Ome As 0 CUpTCe EEi ETh S SSoRn wSa [Le 3 ImAn 8 di Laeben f1l rxpmeraernd E23atHeEmRaA iro bra S ERa E]EaTmSeYn I5T CNfaamve 15 TTemhavVaaaedoonn pEeTlr-- eid i5 SPaore 5 TTeomeaivViaenmseosn.s| 45 DSeeetna connsamn Peres 002224 x2- Wi Ld PROJECTNO.: 454-110 Appendi3x Cer(tcointfoinfiuAecnda)alytsies. CeIntNreTAEnaRlyItiMcalCLEabRoTrCaIOtAFRoOefrIeFrieACneNceA#sA:LY0T2S3I-E0S18A DateofLustAnalysis: 0831/00 `ExpirationDate: 083101 `StorageConditions:Frozen 10C Re-sssessmentDate: 083101 `FPluuroirtiyde=,01.005%9%-+(NsMuRmoifmpmeutrailisi,mp1ur.i9t3i%es,o1r.g4a5n%ica+cLiCdIiMmpSuirmiptuirietsi,e0s3,88%.-4+1P%OiAnAor,ganic. 033%) "ToPtuarliitmypur=it1y00f%ro-m 1a3l.l09e%sts==861.3.90%9% Potassium isexpectedinthissaltformandisterefoceno consideredanimpurity. PourbitysbyfeDoSrrtChiivssgsaeemnpelrdea.llynotapplicabletomateria oflow pury.Noendotbewrams "Siulfnuriontahrreisonamgmeptlhaeoadpcnpoenadriisttioocbnse.coTnhveearntieodtnroesSuOltangrdeheesnwceeldlewtietchttehduessilngftrhe d`oenetmhienraetsiounsitnhtehSeeOl4eimneoottalcaonnasliydseirse,dleanndiimngpcuotnyf.idencetothisinterpretation. Based THEFABA TrHiefpluoBruoosrcoebtauciiycdariccd NPFFPPAA NPeonntoafflluuoorrooppernotpaannoacciiicccdd "Theoreticalvaluecalculationsbasedontheempirical formula,CaF SOSK"(MW=538) "ThisworkwasconductedunderEPAGoodLaboratoryPractice Sundards(40CFR 160). consaisn ar 002225 .33- Ww PROCNO: 454110 Appi Cer(tcointofifniuAencda)alytsies Centre Laboratories, Inc. SAoN nv ar F ee Fishe , r nie ts INTERIMCERTIFOIFACNAALYTSIES Fr e -- e -- er Ce t -- e r ------ a -- ---- L IE E -- ra -- o -- s-- ey Tl roi EpEeA ree he enGA EL lllplN e]o.iall -- res " 002226 ~ Wildiste I Led. -- PROJECTNO: 454-110 Appendix 4 `TheAnalysisofPFOS In Soil and Seven SpeciesofPlants In Support of `WildlifeIntemational, Ltd. Project No.: 454-110 002227 -35- _--M--_o;~--m--Am--Am--AmAeemA -- A--A -- ---- PROJECT NO.: 454-110 INTRODUCTION Soil and plant tissue (vegetative and fui) samples were collected from a plant toxicity study designed to determine the effects of Perfluorooctanesulfonate, Potassium Salt (PFOS) to seven speciesofplants. Thisstudywas conducted by Wildlife International, Ltd. for 3M Corporation and identified as Project Number 454-110. The chemistry phase of this study was conducted at the `Wildlife Intemational, Ltd. analytical chemistry facility in Easton, Maryland using high performance liquid chromatography with triple quadrupole mass spectrometric detection (HPLC/MS/MS). `Sampleswerepreparedandanalyzedbetween November 15,2001 and Aug2u8,2s00t2. MATERIALS AND METHODS `TestSubstance The test substance sed for this study was Wildlife Intemational, Ltd. identification number 4675. `Thetestsubstance wasusedtopreparecalibrationand matrix fortification samples. AnalyticalMethodology Artificial Soil Submitted artificial soil samples were analyzed for PFOS following procedures documented in Wildlife International, Ltd. study number 454C-120 entitled "Analytical Method Validation for the DeterminationofPerfluorooctancsulfonate, Potassium Salt (PFOS) in Artificial Soil" (8). The eatire submitted soil samplewasblended for approximately two minutespriorto `sub-sampling the requisite 10-g aliquot for the analytical determination. The sub-samples were extracted with methanol, agitated for a minimum of 30 minutes on a gyratory shaker table at approximately 250 rpm, and vacuum filtered with qualitative filter paper. The retained soil was wriple rinsed with methanol. The filtrate was then transferred to 200-mL volumetric flask and `brought to volume with methanol. Approximately 20 millilitersofeach sample was transferred to a separate vial or tube and centrifuged for approximately 5minutesat 2000 rpm. Dilutions into the calibration rangeofthe HPLC/MS/MS methodology were performed with a solution of 50% `methanol (HPLC grade, 99.9+%) and 50% NANOpure water. Samples were then analyzed by direct injection. ConcentrationsofPFOS were determined by reverse-phase high performance. liquid chromatography using 3 Hewlett-Packard Model 1100 High Performance Liquid Chromatograph (HPLC) with a Perkin-Elmer API 3000LC Mass Spectrometer equipped with a Perkin-Elmer TurbolonSpray ion source. Chromatographic separations were achieved using a -36- 002228 --nm-- 1++-------- PROJECTNO: 454-110 Keystone Betasil Cyscol(u 50m mmnx 2.0mm, 3pmparticlesize)fittedwith a Keystone Javelin sCoyslgiuaprrdocviodleudminnA(p2p0emndmixx42..10amnmd)t.heiAnsmtertuhmoedntfplaorwacmheartterfsoar ePFsOuSmmdaertierzmeidnaitnAiopnpseinndaritxif4i2c.ial Plant Tisue Submitted plant tissue samples were analyzed for PFOS following procedures documented in Wildlife Intemational, Ltd. study number 454C-125 entitled "Analytical Method Validatio Tissues" n for (9). the Determination Vegetative (ie. ste of ms Perfluorooctancsulfonate, Potassium and leaves) and fui tissues were pr Salt (P ocessed FOS) and in Plant analyzed using the same procedures. The entire submited plan sample was manually shredded, cu, andlor blended as spproprise Ifsufcient samplequantitywas svailabl, on-gram aliquots of eachhomogenatewereweighedintovialsforanalyticaldetermination. Numerousstudysamples (ie. Day 21 vegetative tissues) were quantity limited. Therefore, the folowing crteion was implemented: IF given replica (A,B,orC) for test level contained 20.5gof issue, the replicate was individually analyzed. Iftwo or mr replicates contained <0. g, th replicates were composited. Composite replwierceaanatlyezesdwhenthecompositeweightwas20.55. Plant subsamples were extracinttehed vias withten millers ofmethanol. Extraction consiofsmatneuadl shakingforapproximateolney minute followed by spproximately 30 minutes ofagitation ongyraoryshaker tablesetat approximately 250 pm. The sub-sampleswere then cenifuged for approximately ten minutes at approximately 2000 rpm. Dilutions into the calibration rangeofthe HPLC/MSMS methodology were performed with a solution of 50% d`mierthtanionlje(cHtiPoLnCusgirnagdet,h9e9s.a9m+e%)iannstdru5m0en%taNlAcNoOnpdiutrieons water. Samples were as described for the then analyzed determination by of PFOS in soil (Appendix 42). Amethodflowchartfoth determinationofPFOS in plant tissues is provided in Appendix 43. PrSimaeryacnd ondStoackSorlutiyons A primary and secondary stock preparations were adjusted for the purity of the test substance (86.9%). Details on the preparation of the stock solutions of PFOS are provided in Appendix 4.4. The primary andsecondarystocks were usedfporeparationof calibration standards and matix orificaion samples a. 002229 _--_-- Wd Lid PROJECTNO.: 454-110 `CalibrationStandardsandCalibrationCurves Calibration standards of PFOS, prepared in 50:50 methanol: NANOpure water by `appropriate dilution ofthe 0.00100 Wg a. WL stock solutionofPFOS in methanol (Appendix 4.4), were analyzed with cach soil and plan tissue sample set. For soil analyses, PFOS calibration standards ranged in concentration from 1.00 to 10.0 pig a.i/L. For plant issue analyses, PFOS calibration standards ranged in concentration from 0.400 to 5.00 ug 8./L. The calibration standard serieswasinjectedatthebeginning andendofcachrun, andonestandard wasinjected,at a minafeirevm eryfuivesmampl,es. ThesameandmostprominentpeakresponseforPFOSwas utilized to monitor PFOS in al calibration and study samples. No attempt was made to quantity PFOS on the basis of individual isomeric components. Linear regression equations were generated using peak ara responses versus the respective concentrationsofthe calibration standards. Typical calibration curves for PFOS determinations in artificial soil and plant tissues are presented in Appendices 4.5 and 4.6, respectively. Representative ion chromatograms of low- and high-level PFOS calibrationstandardsarepresentedinAppendices 4.7and4.8 The concentrations of PROS in the samples were determined by substituting peak area responses into the applicable linear regression equation. Concentrations were calculated on a dry weight basis using moisture determination data submitied with the samples. An exampleofthe calcufolaaretprieseontantisve artificial soilsampleisincilnAuppdenedixd 4.9. LiomfQuiantittatsion ArtificialSoil `The method limitofquantitation (LOG) i artifical soil was 1.18 mg 8.i/kg on a dry- weight basis andwascalculatedastheproductofthelowest calibration standard (1.00ig aL = 0.00100 mga.i/L), the weight/volume dilution factor of the matrix blank samples (1000) dividedbythepercentsolid forthetestsystem soil (84.9%). Plant Tissue- Fruit Method LOQs in plant fit tissues were reported on a dry-weight basis and were calculated for each plant fruit species, except alflfs, as the productofthe low-level calibration standard concentration, the weight/volume dilution factorofthematrixblank samples, divided by the percent solids determined for the fruit species negative control samples. The presence of -38- 002230 _-- Ww, PROJECT NO: 454-110 contamination andlor inteferent matrix specie in alfalfa fruit tissue necessitateda unique LOQ definitionrelativeto theotherplant rut species. For alfalfa fruit, the LOQ was calculated on a dry-weight basis as the maximum mg PFOS/Kg equivalent concentration observed in the negative control extract rounded upward to the nearesttenthmg a.i/kg value (sec LimitsofQuantitation. PlantTissue- Vegetative). Plant Tissue- Vegetative MethodLOQsin plantvegetativetissueswerereportedon adryweightbasisandwere calculated as the maximum mg PFOS/Kg equivalent concentration observed in the negative control extracts forcachplantvegetative issuespecies,roundedupwardtothenearesttenthmg. 2i/kg value. As was the case for alflfa fruit, unidentified matrix interferences were often evident in selected negative control samples at levels above the low standard equivalent LOQ definition (sce LimitsofQuantitation, Plant Tissue ~ Fruit). Further, the magnitudeofthe matrix interference was highly variable within a set of negative controls for a given vegetative tissue. species, thereby negating useofan averaged background correction approach. MaFtroixrtificanad MtatirixoBlnansks ArtificalSoil Selected 20.0-g aliquotsofartificial soil matrix were fortified with the appropriate stock solutionsofPFOS prepared in methanol using a gas-tight syringe. The fortified soilswerethen `homogenized with blending forspproximatclytwo minutes. A 10-5 aliquotofeach homogenized fortified soil sample was weighed into a tared weigh boat and transferred to an 8-02. Frenchsquare botle for extraction. The matrix blanks were unfortfed artifical soil. Along with the sample analyses, three matrix blanks were analyzed to determine possible interferences. No interferences were observed at or above the LOQ during the sample analyses (Appendix 4.10). A representative ion chromatogram ofa matrix blank sample in artifical soil is presented in Appendix 4.11. Arificial soil samples were fortified at 2.36, 118, and 1410 mg a.ikg (dry weight bass) and analyzed concurrently with the samples to determine the mean procedural recovery. The `method yielded mean procedural recoveries of 83.9% 89.0% and 86.5%, respectively (Appendix 39- 002231 ---- W, Lid PROJECT NO: 454-110 4.10). Sample measured concentrations were not comected forthe mean procedural recoveries, A in representative ion Appendix 4.12. chromatogramof amatrix fortification sample in artificial soil is presented Plant Tissues withappr`Soeplreicsteedstaloicqkusootlsu(taipopnrooxfimPaFteOlSypr1.e0p0arg)eodfinnegmaettihvaenoclonutrsoilnpaglagnatst-iisgsuhessywreirnegef.orAtified matrix blankwaspreparedwithunforifednegativecontolplanttissue. Fruit and vegetative plant tissue samples were fortified at target nominal concentrations 0f0.050, 0.50, and 50.0 mg a.i/kg (wet weight todetmeeanprrocemdurail rencoveeries.Inf basis) ut tis and sue, analyzed concurrently with themeanproceduralrecove the samples riesranged from 86.3 to 117%ofnominal concentrations (Appendix Werenotcorrectedforthemeanprocedural recoveries. 4.13). Sample measured concentrations Repreisoenchnrotmaatotgraimsvofea matrix blank and a matrix fortification sample in a plant rit issue are presented in Appendices 4.14204 4.15, respectively. In vegteissut,thaemetan iprocvedueralrecoveriesrangedfom 88.0 10 110% of nominal concentrations (Appendix 4.16). Sample measured concentrationswerenot comected for the mean procedural recoveries. Representative ion chromatograms of a matrix blank and a matrix fortification sample in a lant vegetative tissue ar presented in Appendices 417and 4.1,respectively. RESULTS Acificial Soi `Samplesofarial oilcollectedonDays 0and21,andattettermination weresubmited and analyzed for PFOS. All negative contol artifical soil samples were <LOQ for PFOS. Day 0 analyses ofthe 3.91, 15.6, 62.5, 250, and 1000mg kgtreatment eves had mean measured values of3.61, 11.1, 508, 276, and 998 mg a... comresponding to 92.3%, 71.2%, 81.3%, 110%, and 99.8%of {terminat th ion e nomina samples l concentrations, respectiv are presented in Appendix ely (Appendix 4.19). 4.20. The 3.91, 156 PFOS , 62.5, measured valuesfortest 250 and 1000 mg akg reatmentlevelshadmeanmeasvualrueseofd 1.29, 3.56, 162 157and 474 mg 14g, coresponding 10 33.0%, 228%, 25.9%, 62.8% and 474% of the nominal concentrations, respectively. A representative on chromatogram ofan artifical sil et sample presented in Appendix 4.21 a0. 002232 --_--_--mm--s eooe PROJECTNO.: 454-110 lantTissues `Samples of fruit tissue from five speciesofplant (alfalfa, flx, onion, soybean, and tomato) growninnegativecontrol andPFOS-treatedartificialsoilswerecollectedattesttermination(for a given species) and analyzed for PFOS residues. Samplesofvegetative tissue from seven species of plant (alfalfa, flax, lettuce, onion, ryegrass, soybean, and tomato) grown in negative control and PFOS-treated artificial soils were collected at experimental termination (for a given species) and analyzed for PFOS residues. Measured PFOS concentrations are presented in Appendices 4.2 -- 433. Representative ion chromatograms of plant fruit and vegetative tissue test samples are presentiend Appendices 4.34and 4.35, respectively. a. 002233 e --_--e _--m-- -m A ,--,-------------- W, PROJECTNO: 454-110 Appendix 4.1 `Analytical Method Flowchart for the Processing ofPFOS in Soil 20.P0regpoafresoQilCmsaatmrpilxefsorien soialQmCactirnitxhofrtometdheweapipgrhopbroiaatt.eRmeecthoarndowleisgthotcsks.. TWreaingshfaetprpr8-o0x2i.maFtreelnych isnqumaertehaglnaoslswboittthles.gasF-otritgihftyscyaricnhges.oilAslalmopwlteoawiirthrtyh.e aTphperompartiratiex lvoalnukmeios ufnfPoFrOtSifesdtoscokilsmoalturtiixo.n Forcach QC and testsample,transfer the entire 4 contentsfrom the 8-0z Frenchsquareglassbotle to a blender. Homogenize cach sampleforappzroxoimaetel.y 2 minutes. Place the entire homogenate into `Weigh approximately 10.0 grTarmsoafleaeceh studr y samplq e intoaotiareed weigh boat. Record weights. For each sample, measure 100 mLofFrmeentchhansoqluawriethboatgr.aduated cylinder and transfer into the Cap bottles and place on shaker table. Al4low the samples to shake for a minimum of 30 minutes at approximately 250 rpm. Vacuum ite with gulaive iepaper ad i1e resin il 3 times with methane te `Transfer the flrate intora 20w0-mL volurmeptrsicrlaeskpasnd birinsg to volume with methanol. "Transfer apvpiraolxainmdatcealpy.2C0emntLrioffuegaecshamspalmepslfeorinatpoparosxeipmaaratteelyla5smsicneunttersifautg2e0t0u0beroprms.cintillation Prepare appropriate ilution(s) o bring final co+ncentration ino the calibration rangeofthe LMS. `methodology. For all diilguthitons,riusneg5s0%odmeCtlhoasnoAlv:o5l0u%meNtArNiOcpgluarsscwarwea.ter dilution solvent, gas- Ampulate and submit sample for HPLC/MS/MS analysis. 002234 -a2- - nnn Appendix 4.2 Type HPLCMSIMS Operators Pmt "INSTRUMENT: 1oN SOURCE: HCSepwhelrceohtomim-aePtiaoecrkgaOrrpdeMrotdeedlP1mo1i0ln0HFieighsPoenArPfrToer3sm0ao0n0nceMcLoiqiuidg (MRM) mode. PeinEimerTubolonspray ANALYTICAL COLUMN: Keystone Betasil Cyy (50 mm x 2.0mm, 3-um particle size) `GUARDCOLUMN: OVENTEMPEATURE: sropTE: a5r0cmin Keystone JavelinCyscartridge (20mm x 2mm) FLOW RATE: 250 uL/min MOBILE PHASE: To30% ea NANOpure Waterwith0.1%Formic Acid: NECTION VOLUME: sou FReOsSPEAK RETENTION Approval mines . PFOS MONTIORED MASS: __ 4590 amy 99. sms 002235 _-- w PROJECTNO: 454-110 Appendix 43 Analytical Method Flowchartfo the Processing ofPFOS inPlantTissue Rbleemnodivneg,saasmpalpepsrofprrioamtef.rozWeenisgihratghee.apHproomporgiaetneiuzembsaemrploefs1witahlimqaunoutsalofsharcehdihnogm,ocguetntaitngeainndo/oar tared vial, i Qutailsisutey.coFnotrrtoilfysammatprliexsfaorrtipfriecpatairoendsraompmle1s wiatlihqtuhoetsaopfprhooprmiosgteenPiFzeOdSnsetgoactkivsoelcuotnitorno(ls)plinant `methanol using gas-ightsyringe(s). The mairix blak isunforified plant matrix. 1 For cach samCpalpe,thmeavsuireaa1n0d.ls0hmaskLeomfamneutahlalnyofolrwiatmh ailasns CoilfasomsnAemuivnoulmtuem.etric pipet. + Placesamplesongyratoryshakertableandshakeat asetingof 250rpmfor approximately thirty minutes. + `Centrifuge the vias at approximately 2000 rpm for approximately 10 mines. 1 caliPbrreaptaiorne raapnprgoeoprfitathee dPiFluOtSionLsoCfMsStumdeythaonddolQogCy:samPaprlteisaltloy bfirlilnCglcaosnscAentvroaltuimoentsriwcitfhliansktshweith 5a0g%a:s-t5i0gh%t mseytrhianngeola/nNdAbNriOnpgutro cvowlautmeerwdiitlhutdiiolnutsioolnvesnotl.venAt.ddParpopcreospsrimaatterivxoblluamnekofsasmapmlpelseswiintgh tsheevesraamleredpielauttiionnvaernsdioanlsi.quoSttovroeltuhmeersemasafionritnhgeorliogwiensalt mfoertthiafincoaltieoxntlreavcetls. iCn athpeawnadlmkiixn wceoolllerb.y + "Transfer an aliquot ofeach sample 0 a autosampler vial and submit for HPLC/MS/MS analysis. 002236 a4 -_-- Ww Lid PRONJO.E45C6-1T10 Appendix 4 Analytical Stocks and Standards Preparation A 10.0pg2.i/uL primary stock soluotf iPFoOnSwas prepbyawerigehidng 1.150g8ofthe tmeestthsaunboslt.ancSeeocnonadnaraynalsyttoicckalsboalluatnicoen.s T(h1e00t,es0t1s0u0bs,ta0n.c01e0w0a,s0b.r00o1u00g,httaon1d000-.m00L0f1i0n0alpvgoiluume)witohf PROSin methanol werepreparedfrom theprimary stock byserialvolumetedilution. The csahloiwbrsatdiiolnutsiotnansdcahrdesmewsefroethpreepsartesdoifcnal5i0b%rat(ivo/nv)smtaentdhaarndosleimnplNoAyNeOdpfuoredetewramtienr.atiTonhoeffoPlFlOoSwinign soil and plant issues: ConcSetnotrcaktion' (u00g00a00i11/00u00l) 000.000000111000000 Calibration Standards for DeterminationofPFOS in Soil Aliquot VFoinlaulme Con`cSetnatnrdaartdion w 2150l00 (1m010L00) (g21a50i00l) 1570050000 111000000 571.500000 Calibration StandardsforDetermination ofPROS inPlant Tissues ConcSetnorcaktion ~~Aliquot VolFiunmale ConScteanntdraaztaion g00a01i0l0) o0l0) m1L00) 000100 500 100 (g0a4i00l) 0500 000000110000 21500 110000 215000 000000110000 355000 110000 355000 002237 ase --_-- m sere PROJECT NO: 454-110 Appendix 45 `Typical Calibration CurveforPFOS Determinations in Artificial Soil pCiDn4d20.(01.25)80a.r1Moog ver118B5a1r3c8rd CSloomeai=ontoCogsgts.h=to0n98e738 IS a 1006 so oa "oa x 0 ome asm oi wn ww om mguin. 002238 -46- --_-- PROJECT NO.: 454-110 Appendix 46 `TypicalCalibrationCurvefor PFOSDeterminationsinPlantTissues. WPeRiOgSh.t4ed5(105) I.n0Nto-r=2ec2r79ae.l4pS505ta.0n1d oSmoa=nSepCzo8o6t2a6.2= 0.59864 os aoc reo 1400 1200 1000 800 soo oo 200 ook ia he ade sw se ak 4k Covngaein 002239 a7. - 00000000 PROIECTNO: 454110 Appendix4.7 B5E B LHp T pRe%eii.z a e s.1 e m:i.: E SO Representative Chromatogram of a Low-Level PFOSCalibrationStandard EEn,e p: an : aw =. SB Bn hE BE Nominal onsenation: 0400 ga... I" 008240 Wa Led OO PROJECT No 454-110 Representa f &f imons BPmPi payra-- = FE.on aT= FiorBse,n l830h EEgnme" e iaomn Segre atonTi.pe hA-iV3E Aope8n3 Ciomstogam of ighLevel FOSCalibrationSiandrd er oo : ee non .E: -br ;k. yun o_o we OteTeth EHho Wo Wh1 Waoa 2TSiamn Nominalconcenaion: 100 gai. 002241 -o- EE w roc. sto Appendix4.9 Example Calculations foar Representative Sample. The analytical resultandpercentrecovery ofPFOS in artificial soil for sample number 454-110-10, `nominal concentrationof62.5 mg a.i./kg, was calculated using the following equations: c==0 -- 7 Dobe C = concentration The est osoCa o OM8I1Shn eOifei ol slnQmui-- dissl-- s: ConcenPtFOrS(amtgai.io/kng) = Wx ) (Vi) x (Viax) (%Solids) SO ---- V, =Extraction Volume (100mL) Vii = First Initial Volume (100 mL) Va =SecondInitial Volume (0.0500 mL) VVaa == SFiercsotnFdinFailnaVloVloulmuem(e20(050m.L0)mL) ots xinacon W = Weightofextrsaampcle(t10e.0dg) `Concentration PFOS(mg &.i/k8) = "10.x0100x0.0500 x 0.845 eCooa nnceintnraCtiol on PFnOSn(m-g585.Ei0G/kS5g)tE=og5Sa7.60 neme e 257.60 0m Calculated with HPLC/MS/MS instrument software: MacQuan,version 1.6. 002242 _--_-- Woldiste I Led PRONJO.E: 45C4-1T10 Appendix 4.10 Astificial Soil Matrix Blanks and Fortifications Analyzed Concurrently During Sample Analysis. (54a-m1b1e0r) MMAABB--31 Sample Tipe SSooiill MMaatrrixx llaannkk. SaImneprlali_n_g S(megaeifmg Forified Measured! Temmiitnoinon~~ 0000 <<Ll118g" Percent | MMowewnred Recovered' (mg ifkg) -- <Z1 MPeeraennt Recovery - MMAASS--17 SSooiillMMaatrriixxFFoorrtiiffiiccaattiioonn Temrmainiatnion 223366 212909 95s.e1 2 9s MMAASS--28 SSooiillMMaattrriixxFFoorrtiffiiccaattiioonn TeIrnmtiantaitoinon 111188 110084 98113 106 98 MMAASS-=39 SSooiill MMaattriixx FFoorritiffiiccaattiioonn TeImiitnagtioonn 11441100 11321700 120 1250 as - TT SundourmdiDetviation= 3w.m78 Measured ndPercentRecvoaluvesweerercalecu aded wing MacQues,version 1 wNee software. a MmaT caleoaylvaaryoSinghst "t`ThRehesleuolmtwesetrshetopocdrlltiiebmdiortinoofanqdsuratynatnwidetaairtgdi(hot0nb.a(0sL0iO1s.Q00)imna2rt.i1fLi)caalnoditlhweawsei1g.h1t8'mvoglumedkigloutnioancrayc-twoerigohftthbeamsaitssrinxdbwlaanskcaslacmupllaetsed(a10t0h0e)pGriovdiudcetdobyf thepercentsolids forthe tetsysemsoil (84.9%) 002243 -s1- 0000000 PRONTO E4sCt1T10 Apeia12 ReservCromtogamaf1 Afi]Si Mas FrictionSample To we mmm FEieeoe " on P= E ow :; EBEERRL: .`. w Frage p Err x EH :hap pn . untenie A-v0 om iw 2m Zw aw dn one SNourpie eCnoinfeainornn::4151431m10g.M4.AS2 rywightbs). a 002244 _-- PROJECTNO.: 454-110 Appendix 4.13 FruitTissue Matrix Blanks and Fortifications Analyzed Concurrently During Sample Analysis. -Supe Nsaeboe)r Type SIanmtpelrivnagl Con(cmegntxriaftkigo)n Fortified" Measured WeBVasiiesgh DyBWuseigh RePcoveereed! WWREFEMMAABS-3L AAllffaaMFaotrrtiifxicBaatirokn TeTremriminnaattiioonn 50000 3S0049 s1a38t 0- AACCEMMAASBTL IGCEEMMAASSS2 FFllaaxx MFoarrtiisfeBaltaionnk FFllaaxx FFoorrttiiffiiccaattiioonn TTeerrmmiinnaattiioonn 0000495 TTeerrmmiinnaattiioonn 0a5s03 <00o5o810 o<omnz 1 0"2a1 11502 21014 Non-i-FGMMAAB-SL OOnniioonnMFaotrriifxiBcaltainonk TTeerrmmiinnaattiioonn 0004050 Hon--FFMMAASS32 OnOniioonn FFoorrttiiffiiccaattiioonn TTeerrmmiinnaattiioonn 0a4s67 P<roeod a<s0aa2 7-3 0s4iset 4EsY 91%067 sSooyyFEMMAABS-]1 SoSoyybbeeaannMFaotrrtiifxiBclatainokn TTeemrmiinnsatiioonn 0005000 ys0FyMFAMASS32 SSooyybbeeaannFFoorrttiiffiiccaattiioonn TTeerrmmiinnaattiioonn 5004506 <ooosoo0s 0<2om2s 1-02 0a4s78 2909 995674 ftoommCFMMAASB--|1 TToommaattooMFaotrriixfBclaaionnk TTeerrmmiinnaatiioonn 000408 tOommEEMMAASS23 TToommaatoo FForriifisctatoionn TeTremrimniantaitioonn 04a9s5 0<.o040608 0<3o544r 9-39 oaa3 s5o0r5 88683 5 T"hTihceumaertehdosdndiiPetrocefnqtuaRnetciotvateiroend(vLaOlQue)siwnefrietctliossauiewdawscinaglMlaeodus,theveprrsoidounct1o6fmhfevlaowreestMcaanliiabrcaonlsansdalrdm(aa0y.0vi0a0r4oy0S0mnhgsy 31/F1)aondPFrhOeSwiceognhfcteivniosliuoemnesddaileatpornesanttoerdoonf t8hwemeawreiibglhanbakssia.mple. "T`hwaesmceatlhcuoldatledmsssotfheqmuaanxiitamtuiomnm(gLOPQF)OSi/naKgfeqluivfaltenitsscuoens(e6.n4rmagion mkegasoun r3edirnyn-ewgeaitgihvebcaosntsro=l2s.a3mpglae.extractonroauwndeed-uwpewiabargstidosh) the nearest tenn mg 21k. 002245 -54- 0 PROECTNO: s5410 Apentcara ReprsnatofvPletFCr TliesMuaimlnakgSpal mTpohrre em EFaaris " eynn Np EBB2oeFTE%egs. m ]ii20 :h.o R2FoE0g),54 :; l EB=EEErr. =E . RRE GH : e AER EN ------------y Sample Identification: 454-110-SOY-F-MAB-1 (soybean matrix). se 002246 - 0 66 PROTECTNO: 454-110 [e---- RepresentativeChromatogram of aPlantFruitTissue MatrixFortificationSample. Te -------- EFpreom . ry Dd : BEBBREREIi| ;: : aTBmez p: T Mugenwin, 087 " 49 81 1 wg XR 2 head JEL Be te Uh Wh BH AW THER Sample Identification: 454-110-SOY-F-MAS-3 (soybean matrix). `Nominal Concentration: 50mg.a.i.0/kg (wet.weight basis). 002247 5. --_--mmm- / PROJECT NO.: 454-110 Appendix 4.16 VegeTistsueaMattriix Bvlaneksand Fortifications Analyzed ConcurrentlyDuring Sample Analysis - Sample -- Naber sein) Toe ISnaemrpvlianlg Concentration mai Forties" Measured WeBaWsei gh DyBWusaigh RePcoeveersed! AAlEfVYMMAABSL3 AAllallfs FMoarsifiicBaltanokn. TTeerrmmiinnaattiioonn a0l0 MMVvMMAASB3 FFllaaxx FMaotriinfBcaltaonnk TTeerrmmiinnaattiioonn a0s0 lJewVMMAASBZ LeLtetutcceeMFoarsiifaBtiloank TTeermmiinnaattioonn 003002 JWMAS3 LoreeForifcation Temination 04 nonvi--MVMAABS2S OnOinoinonMoartiifxiBcaiinokn TTeerrmmiinnaattiioonn 0001 <"o2s <227 si-s <aosm <12584 3- <003d3%s <osns 1-07 as Ed 9%7 <worn <a21 8-80 MDEEVVMMAABS-2L RRyyeeprrassss MFaotriifxeaBtainokn TTeerrmmiinnaatiioonn o0s0 <4138 <1030 i-o SSooyv-MMAABS2 SSooyybbeeaann MFoarrtiifsicBaltaionnk TTeerrmmiinnaattiioonn 300 <24 <60 - we 1s 1 fToommVMMAABS-3L TToommaattooMFaorrixfcBaainokn TTeermmmiinnaatiioonn 0"0a <w1s4 <276 1_02 meTaheseaumrreetedhdiondnnldeignPaitriovefecqmouRnienttcrcooalvteeixrotnerda(vcLtaOsfQlo)rstwihneacteascchsvaergeoeualtnidwveidniusgpswuMearawdcaQsoutachanle,cvueelraastrieodsn2estth6ehsmomafgxwairmeumMmagnoPaFOuSIKcequiuvamlaeniytvcoanro cyeSntrhnatiyon Fortified PROSconcentrations represeniedon awet-neight ass 002248 57 r--ee-------------------------------------- Ww, PRONJO:E45C4-1T10 Appendix 417 Representative Chromatogorfam Plant Vegetative Tissue Matrix Blank Sample TToehs wer swan man ns En ws Laisea m rs . E anaRB " hE G0 - Ser 2ls ;. mt 170 0 | odeven 38 upninVt 4-5 ET vis son 1200 see to the WH Wh Th UW WE `Sample Identification: 454-110-LET-V-MAB-1 (eftuce mati). "Thearrowindicatesth retentiontime ofPES. 002249 _ss- -_ FROTECTNO; 454110 RepresentativeChromatogramsAPpspteVndeigxs4i.1v8e Tiss wn we mmm H --n mi iE ;- EFEE iJ = BHiger. L8 : BBe om : ET [AA atiFortinSle oeom " a ax] SNoamipnaliCcicaotionn: 4c50.54em4g1n.-1. 1tM(A0veStr:oLcigEathtoaTo:rm-a.rV). 5. 002250 ro------------------eeeeeeeeretme PROJECTNO 454-110 Appendix419 Day 0RecovrisforPROSinAviaSil mr Sengear oepineg e Pacem MVeeard PMeevemn (454-110) Concentration Measured" Recovered" (mg .i.kg) Recovery a a <u - as - H@ 550 5isns n2fia7 sa 23 os 1i56 112 0nm wa na 01 156 864 554 a% aaass s5u0w0 Ei0He0 s08 as iu i =2500 paiAsn 7a1ss6 6 no iisHw oo1to0o sw4wo 3s0s P Py --_-- SundodDovw mn= m36 yieayhghe, eReRsempeodieoedvyewg ed eg MNaouwve TEoR Ve rotehampnrhketsaoaflerso1tw0es0adcae(rtdoLnisnaOrnpdeGhrd(t0l0ll01w0s0a 31e.1187mt01)w.dseornwdoer(ve9ie%gh bhasiosanndfwosrcoifhced Susi outer i xedfomMe bass con o. 002251 --------ee-- e ------------------------ Wildle Ltd PRONJO:E45C4-1T10 Appendix 420 `Termination Recoveries for PFOS in Artificial Soil = Semple (mguifg) Mean Mean (a#5m4b11e0r) ConNcocmnirnaatilon Measured! RePcoevrenrted|' (Mmgeaiafsigd) RPeacroveenrty x 00 <uigt - <t1s - 30 339911 113216 3u170 129 30 23 115566 parse 32s 356 ns EF34 as3 116613 22631 162 29 33s6 220%0 11651 5ie 157 as 33 11000000 siwns asi2s an ae TT Sundurd Deviosimone= 16.4 oNvee a1n0 Th`imacyavsarreydsl1inghdtlPye.rRceesntulRtescroevpeorreteddvaolnuaesdwreyr-eweaiglhltibaesids.wing MacQuen, version 16 sofware. Manual clealaions 2"5Thtehempertohdoudctloimfitthqualnotwietasttiocnlf(rLaOtiQo)nisntaarntdiafridca(l s0ol.w0ams0g1.8 180.m1g0) ankdtghewoneigdhyt-vwoeliugmhebadsiilsuteinondfwaos cotalfctuohleated mari leksampie(1000)divided bythepercent lidefrth etsytemsoi (84.99) "61- 002252 PROTECT NO: 454-110 Apendix 421 Representative Chromatogramofan Artificial Soil Test Sample mE 0 eens pjhsarr a.is " ew 1 pf m;|rnhEie- os p k BBaEiE.el# :. [=In p 5 hE ee Bam x: J. P EERe. 858 : To ta Hh Hh , BG BE SNaommpilnealeCnotniceanttiraotni:on:546-21.0-m1g0k. g (dy-weight basi). a 002253 Ww, k Lid PROJECTNO.: 454-110 Appendix4.22 `Termination MeasuredValuesforPFOSinAlfalfa Vegetative Tissue Nsaebrieor) Sample NominalExposure T(omgaCing) Measured' MeMaesaurned (mgaifig) alafv120 0000 afi 00 <<2r7 <1 <27 atrave) 33991 alf24 391 <<2277 616 616 aaiitfv22s6 151566 alf+27 156 257646 42 <7 aallffvaa9 as5 alfv30 as 916269 nz ni aaflfv)32 225%0 aifv33 250 11528 158 3 aallfsa) 11000000 -- - rd 1000 z --W_e--ssired aloes wereclelatedusing MacQuan, version1.6 sofware. MaralcalcumlaayvtariySoHnHs. Resa "eTphoerimeedtohnodadlirmiyt-ofwqueanitbgiathsatiti.on(LOG)inalfulfavegetative issue (2.7 m a../kgon ady-weightbasis) wascalculated a: thmeemnaixfiem.ummg 4.1Kequivalentconcentrationmeasuredinnegativecontrol exacts rounded Upward tothenearesttenth Nosample was collecteddo to mortality s thisconcentration. @ 002254 _---m_ Wildlife IL PROJECTNO: 454-110 Appendix 423 `Termination Measured ValuesforPFOS in AlfalfaFruitTissue. Sample TT dsaeriiro) NominalExposure (mTgiasGimge)mmae Measured! MeMaesaurned (gaily) aairree?s) 0000 <6 <64 py 00 aairraes!t 339911 aks 391 aairrees?) 115566 aires 155 -Z <64 <as <64 <64 ariaog) aQss arf as <64 <64 aaegE13)) 22050 pray 20 <6s <64 aabtse}r 11000000 ais 1000 z- - z TReVstsirepcovratlesudeosnwuesrdrrcyawleeciuglahdttedbuassii.ngMacQuan, version 16 sofware, Maral calculationsmayvary gly tphTeeharmeeastmxetitemnhtuohmdmlmigmgiat.PoiFf/OkqSgu./akngteqautiovnal(eLnOtcQo)nciennatrlaftaionfrmueitaissusrueedi(5n.n8egativkecgonotnoaledxrtyrawcetisgrhotubnadseisd)uwpawsarcadlfcoutlhaeted as 2ThAecroempwoassitienssuaffmipclicenwtsaasmmpaldeeweoifaglhlts(a<m0p.l5e)s calvlaicltaebdlefaotnhailsycsoins.cern. #No sawamscopllecltededve to mortaltitthiysconcentration 002255 6 _-- Wildl Led PROJECT NO: 454-110 Appendix 424 `Termination Measured Values for PFOS in Flax Vegetative Tissue Semple TT Nsea1bieor) Nominal Exposure (mrgoasitm)mme Measured" MeMsesuarned (mgaifg) 1v0290 0000 frv2l 00 rfevvs2 339911 rv 391 fxxvva2s6 115566 pe 156 Mxxveva2s9 aass fxev30 as <<224 <24 <24 45s46 495 385 2314 158 70 57477 550 37s fMreavt! 22050 ovat 20 -- - - xfxvvaase 11000000 per] 1000 Z- - 2 TTshieghslsyu.reRdvealluaesrwepeorreteCdolnoudrdywuesiignghMtabcaQsu.an,version 16sofware Mama!caleaatonsmay vary lTehuemlettehaodsdtihmeimaoxfiqmuuamnmtgiKt(agLtOGieYqouiinnvallexntvecgoentoaetniivreattiisosnueme(a2s.urmegd fnnekgagtionvecodnyt-rwoeliegxhttrabcatssirso)unwdiesd. uNpwoarsadmtpoltehewnaesacorlslecetehd dmugto m4a5.rialaitthiys concentsion. There was insufficient sampleweight(<0.5g) availabe or issalysie. 002256 -65- _--_--m-- Ww, PROJECTNO: 454-110 Appendix 425 Termination Measured Values for BFOS in FlaxFruitTissue Sample TFT roS(mgCaiof)mmmMo em n sNaumlbee)r Nominal Exposure Measured" (Mmegsasiufriegd) fxxef2 0000 x3 00 <<0o12 <on <on2 pefrfts 3991 xfs 39 xmesr 1155s6 x9 186 feolo aa3s pes as pxe13d) 2200 afas 2%0 oa021777 0x0 olss o1a0n7 136 241 122260 256 on == - z ax116 11000000 af1s 1000 z- 2 z Tgighhly.eRaevsaullustesrweieporrircedlcoeunlaidderdywuesiingghtMabacsQiu.t, version 16 sofware. Manual lcuationsmayvery w*aTshecmalectuhloatdedliamitthoefpqruoadntuicttaotifonhe(LLoOwGe)sitncfalleixb.rfautiiontsitasnedwaards(0.01.20m04g001mgk4.o4n.1a)sdnrdywtheeightbasissnd p`ewgeaitgihvtevcoolnutmreoldifluuttiotnisfuae (c4ot.f3ot%h)re. matrix blanksples (100)divided bythepercent solids for the 2NThoesre waaswimanssufcpfoilclieelcnttesdaedmpele0wemiogrhtatli(t<y0.a5 5h)isavcaoinlcbelnetrfaotioann.alysis. 002257 -66- ---- -- ---- ---- ---- er ---------------- / PROJECT NO:: 454-110 Appendix 426 `Termination Measured Values for PFOS in Lettuce Vegetative Tissue Sample E (mg aiE kg) Mean saemibieor) NominalExposure Measured" (mMgea2siufrkegd) lleetvve2l0s 0000 eval 00 <<sssst <ss <s5 ltaavvaz2s 339911 lev2s 391 1e2l2s 56 752 lleevz2ss 115556 levz? 156 710556 106 926 lleavv2as aas3 lew30 as latavvaa,r 252500 lavas 250 59889 as 20 :- - z lelve3a4s!! 11000000 lev36 1000 z- - z TRiecvukeseredporvtaedsonwaerreycaweiaglhttedbaussii.ng MacQuan, version 16 sofware. Manual calealsfons ay vary Sight. a"sTthheemmeatxhiomdalmimmitgof8q.u1anKteiqautiivoanl(enLtOcQon)cfenetrtautcieonvmeegeatsautriveeditinssnueeg(a5t.i5vemcgonktroglSaancdrryo-uwneidgehdtubpaswi)dwa0s hcealncuelastted Ttehnethremgwa/skigns.ufficientsampleweight (<0.5 ) available for analysis. "No samples were collected duetomoralaitthyis concentration. 002258 -61- -------------------------------------- Ww PROTECTNO; 454-110 Appendix 427 Temination Messed Values for POS in Onion Vegetative Tissue oa (454-110) wiivvvaialo ppuetnesd seme Nominal Exposure Ia00 53391 ommneism Measured' <@pir &&<r MeMaemnwed (mg ai/kg) <r <r pauitvzeas]e i1si5ee6 &s4s 10s oni-v-28" 6s me oni-v-29" a6s ppoJusttee-- =i2o0 - - z- z- . : oni-v-34 1000 oniev-35! 1000 oni-v-36" 1000 - - - - TSTm ie:hRoomsioeavuennTetyLinORVcaosn eveonTsE i (4m7 gVrgon diyvightais vas lll he RTT 518 Sov Soo meee ev om toe oud le na old dr mokofr ergo a is concn. upwardtothe nearesttenthmga.i./kg. There was insufficient sampleweight(<0.5 g) available for analysis. 002259 a _--_--mm Wildite J Lid PROJECTNO: 454-110 Appendix 428 `Termination Measured Values for PFOS in Onion Fruit Tissue Semple TT @asmeb1e0r) Nomina! Exposure S(Gmg i) Measured" MeMsesaurned (mgaifig) oonni? 0000 onf3 00 os1-7 [2 os oonnifsd 339911 oni' 391 osis1s an % oonnie?8 115566 on 156 <1097 22 26 oannii-f1101) aass onif12' as onoifa13!! 22500 onlst 20 -z - z -z - z oonnik1F67"t 11000000 petary 1000 == - = Toseu xrepvosarliueedasownerrrecyewleeiugdhetdbawsiengMacQuen,version 16 software. Manualcalculaions ayvary Sghly. Tcahlceumlaettedhoadsthmeitproofduqcutaontfitthaetiolno(wLesOtGc)aliinborantiioonnsfuintdatrisdsu(e0.w00s0400.042mmggiaLk)gaannd thderwye-iwgehitg/hvtobalsuimseadnidluwtaiosn actor FThtehreemawraxs.ibnsluafnfikcsiaemntplseasmp(l1e00w)ediigvhid(e<db0.y3tgh)eapveariclebnltesfoolridasnafloyrstihse. negativecontrolfu tisue(9.65%). "No sample wascollected due 0mortalitory lackof fritproduction a this concentration 002260 -69- -_-- Ww, 4 PROJECT NO.: 454-110 Appendix 429 `Termination Measured Values for PFOS in Ryegrass Vegetative Tissue. - Sample TT (asm&1b1e0r) Nominal Expose (mgvo8is/okgm)ema Measured! MeMaesuarned (mg aifkg) evo Hev20 0000 eval 00 <<33 <33 <3 eevvs 339911 Hei 39 577103 sis 70 neevvass 115566 prey 156 8103 3a <33 nyeveaas Heo aa3s as 0as 87 367 eevzal 2200 evs 2% 3379s 1 129 reevvaass 11000000 eva 1000 -- - z TTseicpsoirreeoddnvadalruye-swewiegehtcblaciuel.ied wing MacQu, version 16 software. Namal aleiaionsmayvary Shy Revs "fsThhheemmmegatxhaiomd,uimmimteofaq.u1a/nktgietqautiivoanle(nLtOcGo)ncienntrryaetgiroanssvmeegaestuarteidvie tninsesguaeti(v3.e3cmongtrolkegxaocnts rdoruy-nwdeeidgphtabrasies)fowaxscanlecaurleastted No sample was collecteddueto moraloir tacyk ofemergence at his concentration 0ozze61 -70- _-- Ww, PROJECTNO: 454-110 Appendix 430 Termination Measured Values for PFOS in SoybeanVegetative Tissue Sample T N(o5m41b1e10r) Nominal Exposure ro (mg aks g) omeMem an e Messured" (Mmegaasiufriegd) ssoayy2s0 0000 sayv2l 00 <<a 60 <60 <60 ssooyyrv2232 339911 soy 2s 39 1007 156 191 ssooyyv2a6s 115566 soy 2? 156 ssooyyv2298 aass foyv30 as a2y3 88 23 797 @3 554 ssooyy3321 22500 foyer) 250 6172s us 103 ssooppvr3as)) 11000000 z- soyv36' 1000 z Te ReMsiuclatssrreedpovratleudesoe nwedrwreyeciaglhltedbassiip .ng MacQuan, vesS on 16sofware Manualn calculations mayvary sgEh.ee cTahlceulmaettedhoasdltihmeitmoafxiqumaunmtimtagti.on1/(kLgOGeq)uiinvasloeynbtecaonnvceegnettraattiiovnemteisassuure(60imgnegaatkivgecoonntadolrexyre-tswrbeoausinidgwedahsupward to hNeonesaarmepslteweanshcmogllekcgtd.eude to morality athis concentration. 002262 - EEE -- PROJECT NO.: 454-110 Appendix 431 `Termination Measured Values for PFOS in Soybean Fruit Tissue 5N1ar1b0er) ssoopyf2 opf3 ssoppffs Sop ssoopp7 sop ssooyp1110 Sopf12 ssoopyf-11d3 soyf1s ssooyyH1167)) sopf18 Sample oSCa (mg ae ig) mmw Men e `Nominal Exposure Measured' (Memagsuarge)d 0000 <<0018 <ois 00 <ois 339911 00333497 140 31 291 115566 a1.s63h om 156 0328 aass LLiia 120 as 133 225500 513567 321 250 249 11000000 z- - 1000 z TsopNortie ocnva+ladureyssewweeirugehctrlbcasueel:aeddusingMacQuan,version 16 software, Manualcalculations mayvary sigRheslay. c*aTlhceulmateetdhsodtlhiemiptroofduqcutanotfitthaetiloonw(eLstOQca)liibnrsatoiyobnesatnarndiatrdi(s0s.u0e0w0a4s000m.1g8 .m1gLa.)a./nkdgtohne awedriyg-hwteiivgohltubmaedsisluatnidonwafsactofothre *`mNatorisxabmlpalnekwsaasmcpollelsc(t1e0d0d)udeivtiodemdorbtyatlhietypearctehinstcsooncleinctfroattihonenegative control rit ssue (22.9%). -7m2- 002263 ---------------------------------- Woildiste I Ltd PROJECTNO: 454-110 Appendix 4.32 `Termination Measured Values for PFOS in Tomato Vegetative Tissue -- (454a-m1b1e0r) ttoommevv--2109 tomv-21 ttoomm-vv--2223 tomv:24 ttoommevv--2265 tomv27 ttoommvv--2298 tomv-30 ttoomm--vv--3321) tomv-33 ttoomm-vv-.3354" tom-v-36" Sample. `Nominal Exposure 0000 00 339911 391 115566 156 aass as 225500 250 11000000 1000 (mT gaifr g) stmeMeam n e Measured' (Mmegasnuikree)d <<166 <76 <6 <<7766 <6 <6 122759 ns 61 "049s 01 349 -z - z -- - z TTreWipcosrutreaenddvadalyueeswewirgehtcabalseisu.iidwing Macuan, veon 1 sofare. Manual calaey vaa rySii giloRenses tThehmeamxeithmoudm lmimgitao.if/qkugeaqnuiiavtailoennt(cLoOnGce)ntinrattoimoantomveeagseutraetdivien nisesguaeti(v7e.6comntgro&l.e1xktrgaocnts roudrnyd-ewdeiughptwabarsdist)owathsenceaalrceusltatteedndahs Tmhgeariekweas insufficientsampleweight (<0.5g) available for analysis. "Nosample was collctedduetomortalityor lackofemergenceatthisconcentrtion. 002264 -73- --_--_--m--m--mmm--ee PROJECT NO: 454-110 Appendix 433 `Termination Measured Values for PFOS in Tomato Fruit Tissue -- aNsaambienr ttoomm12 tom 3 ttoomm4s" tom 6 ttoomm 87 tom 9 ttoomm 1101 tom 12 Semple TTT Nomina Exposure 0000 00 339911 391 115566 156 aass as (mTg i2iSsmkgm) me Measured! <<00a47r <047 <0-) <041 <105417 0380 <004457 <041 MeMaseuwred (mgaifig) <047 <047 108 046 ttoomm 1143) 252500 tom 15 2% woomm 1176! 11000000 tom 18 1000 z- - - - - L- Thpeosrstuedreodnvuadlureyswweeirgehtclbacsuei.ed sing MacQuan, version 16 sofware. Mama! cleuations may vary Sights.Reods ab*5lTathnhekemspeatmrhpooleddsoum(f1ict0th0te)ofldoqiwvueiesdne:tdicaabliyibotrnah(teiLopOneQrcs)eainntndsatoorlimdda(st0fo.o0f0th0ue40t0inmsesgguaetw.iavLes)c0o.ni4tn7rodmltfheau.wigeitgihsotenv(oa8ld.ur4y5m%-e)w.ieilgthitobnafsaicstaonodfwahse cmaaltcuilxated "TNhoesraemwpalseiwnsscoullefctfesdaidmcpuleiewteominogrthtatli(t<y0.o5 g)acakvaoifaebmleerfgorenacnaelayfsithsisconcentration. 002265 ne _-- Wildiste I Lid PROTECTNO 454-110 Appendix 4.34 RepresntaiveChromatogram of a lantFiTissueTetSample TE een aDssonnasmsiis dims aEF f mniit aeen N18i2 Tame EHiAos"oe 7 i E RE e O$R7 Tem " rr nc .2p pp as . i" RAEEE EE] `Sample Identification: 454-110-SOY-F-15 (soybean matrix). 002266 75. _--mm_ Ww, L Lid PRONJO:E45C4-1T10 Appendix435 RepresentativeChromatogramof aPlant VegetativeTissueTestSample foes ewan sein amon i vnsaris any 170)co ae54n8t7ems 25s2a0 SEimaehnm E31S F A ontI236 Ee Los Ebt ixtoneievon 6a5"m7 FJaugcatenrTso.w A-S8E8 . - a B'E Tte TteouGWh hWBW2Wa eHeE `Sample Identification: 454-110-LET-V-28 (lettuce matrix). 002267 -76- EE W, ---- PROJECT NO: 454-110 Appendix Temperature(0)Environmental CoRnedlitaiiovnesFarid (04) Due Minimum Masimum Men Minimum Musimum Men aor 159 268 197 n2z wwe 163 28 158 Ba7 [I 22 21 In nso 164 20 ns 2 3 neo 166 8 210 2@ nar 164 255 199 z@" nage 163 267 21 2 se 38 sol 164 26 2s 0 5 ry zoo 163 267 199 55 nar 162 70 197 2 st nao 163 20 02 "@2 nar 164 266 24 16 5 na 167 20 28 "s nase 180 29 212 Py uso 167 27 29 a15 wa iss 29 24 3s 2s 5 wager 19 21 29 a 0 PF was 127 256 210 aa wien 182 23 2s se 5 om 16s 2s 213 36 5 a or 16s 268 20 2 ss ow 164 320 29 1 ss. owen 16s 268 206 05" user! 167 24 23 n 5 " veo 167 2 210 @ or 168 25 4 @5 owl 167 u3 199 z7a ooo 166 22 20 16 n 3 001 164 2s 156 z 5 36 wer 164 21 199 3 a wor 163 23 196 &" wiser 166 u2 199 @ weer 16 210 23 se Ey sor 16s 27 108 16 n 3 eon 161 m2 196 5 3 z TTiiT+IshnicetssGarsoonrwhich2fl 5f3 , e ce,1ee 9g6 ,ad 0 bean 2svere n rd a > FARProAcm i Rasdin MToilgehstPeARn)s 11948 11967 ns 14 11s0 21935 11993 1173s 73 ns 1s 3 11621 ns 1 1s wo 161 ns 17 169 ns 154 3 3 169 ns is .7- 002268 _--_-- PROJECTNO.: 454-110 Appendix 5 Environ(mceonnttailnuCeodn)ditions Die MinimumTempMearxaitmeu(mC) Men MiniRmeulmuivMesHsaimmiudmi (9)Mem MTohltemsPeARt ser woo 16s 164 73 24 198 197 16 r) 153 183 mm 2m ar 162 161 76 264 195 104 10 st 2 n n 21 23 wan ie mer iss ns 194 24 196 nn in 2x 5 30 196 1s wan' maser 162 153 27 83 15 13 1 @ 3s 5 2 0 196 er 13 73 192 [EL 29 190 n32 199 10 2 2 02 awe us 24 187 we us 286 189 16 " P) is 3 2 17s 1s 23000 148 73 16 aver 4s 72 156 5 2 " 2s 5 3 22 oor ow 1e7 us 20 20 187 1s 5 PF 20 n 2 102 ows2 149 51 ns ovw2 19 24 ws 2 5 2 0a 2 13 107 owsn2 149 owen iso 23 191 24 14 u" u ss 25 166 owmz 148 2s 11 owenz 18 265 16 st 0 is 14 193 owen? 149 2s 15s 2 st 38 onions 161 19 196 PY 8 " 154 ns om: 161 om 1s1 29 104 ns 193 ia" 15 st b 168 192 ome omens 162 161 27 25 199 198 n nw 53 a 36 210 1s omsmz iss 7 0 "@ 194 omen: 152 29 196 ony iss 215 198 1s 2 3s >5" 7 186 Ifics Indiasdaascsnwhhiccchh sloolph, nudco, oca gwe,reswoyabeaen,.dr ---- werewares +DDniadtcsdddaaaysssccooonwwwhhhiccehhhfo sap.omnweasi ,srweed. yetweewn, d7Idtitecssdddaaysyssccoooowwwhhhccchhhlllifp,,itassw, erecdes,c1cotianosinndsweeepwsewedr er PARPromptlyAcie Radon 002269 .78- _--mm PROJECTNO:: 454-110 A(cpopnetnidnuiexd) TDuT c viTeewnennwa(O|EMnveimronmentaMl iCoRinedaiwtiwoevnsRaimdtn(M0en grhaety ons ousmz 149 147 263 29 199 102 2 st ss a 201 4 omen 17 262 13 oir isi u7 195 "aa &0 us 1s one? 1s) ns 199 ome? iss 23 197 is El 3 st a3 1s om 16s 25 m4 ose 1s 26 198 @uP ""4 7 26 ost 151 omz 153 76 199 73 199 1" 2o" 211 199 ome om 16 163 1 29 72 28 18 7 st 2 st 196 2 onomz om? 168 167 7 24 27 20 3 ss 25 192 12 oom? 162 4 08 oman st 2 107 = " ss n0 ns 2s omer 150 oan? es 264 197 27 189 15 56 36 153 3 3 omsez 14s ns ws owen? iso 23 195 1 " Pl 15 4 29 oom ow is2 150 21 25 194 108 I @ " " ns 24 owsm2 ovo 150 152 27 268 199 02 1o" 5 ss 26 m2 om: om sa iso 264 20 103 196 1s 2 1 ss 3s 23 193 om 150 om 150 29 197 03 196 mn nu 50 52 22 2s osm: oem: 150 164 21 26 196 28 ss 3 un ss 11 57 ou ies 20 199 on 14s 2 195 1 st 3 2 51 0 2s us osm oom 149 16s 265 23 20 27 n z 5 7s 5 @ 27 15s I oom 6s 26 206 2 " a 156 +dninisssdddayaysssooonnnwwwhhhiiiccchhh Imy,snvderregserige,wrndvo ies.wera e rel ? PARPompei Act Radin 002270 79- _--_--mmm Wildlife I Led ee PROJECTNO: 454-110 (Acpopnteinndueid5x) TR TDeeeMs inimumTw ewMeal xwinmuCmOwEMnveim nronmend taMliCnoinmn duimtioMnsG eimm)Mem many iss 26 02 ome 1s2 79 198 16 n 3 3 3 ou 1s) onsmz iso 20 199 27 0 n El 3 5 " oem 163 ome as 7 23 28 193 2 @ Pp 1s ss oar oom 1s 1s #2 23 195 198 22 n 7s 36 oom st om is? 26 196 251 21 2 n 57 36 oan 147 owsez 14s 70 190 29 190 n 3 2 5 5 30 owenz 152 27 199 oom 162 21 206 I 56 3 nw @ 3 oom 162 m2 2 oom 166 312 2s 17" 2 n 57 wom 159 us 06 ominz is0 23 195 5 " Fl un " 2 own 1st 21 199 ome 165 21 23 2 P " " n ss one 166 20 28 ose 16s 20 2s n si n5 oven ov 164 162 305 21 216 159 3 se 3@" ower ovsn2 162 163 20s 2 108 2 @ ss 2aa ooo 16s oom ist ny 01 29 m1 " n ss zn n a omnia 20 190 omnes 25 198 n 1 2 our osm iss 16s 27 28 2s 6 15 z ss 3 3 ose ome 169 16s 22 26s 6 21 582 2 os ToTonz 160 meiaens er 22 02 2a54n es209 16 53 o 2PnARdPehyey soniwchiaehlsAlcsweeRtvaearts g Molesi PAR 26 02 2s 190 169 27 262 25 m1 168 198 ns 1s 161 163 191 266 10 12 184 10 10 no 1n8e9 162 ns I ur 1s 135 10 7s 1s 11 uz -80- 002271 EE Ww, -- PROJECT NO.: 454-110 Appendix 5 (continued) Environmental Conditions Due MinimumTemperMaatxuirmeu(mC) Mean MinimRemlativMeaHxuimmiduimty(4)Mean onouz 168 27 23 20a 08102 166 24 08 37 % 57 4012 166 24 06 1s i won 167 238 24 2 "" 040302 165 309 20 2 80 51 oon 159 23 203 16 st 2% owosn2 166 261 204 ""7 osu 152 29 203 3"7 owo7i02 157 261 24 3o2 040802 169 254 2 8n" owo9n2 173 23 26 a 73 won? 1m 24 2 2 9 ss ownioz 10 29 20 33a wn? 17s 26 12 "8o own 170 20 21 55n ounanz 187 2s 21 57 3 n wisn 198 300 27 " = o wien 202 27 7 4&7 owmz 195 316 #9 st % ovigm 201 28 #0 5%n oon 195 303 ns 5%n ouz0m2 196 as 80 56 % no oaz102 167 254 207 u 56 oz 16s 24 208 5 n 52 pr 163 255 207 2 El u oun? 113 261 22 2o3 ouasnz 164 %9 209 0 o El 42602 164 24 208 1" unm 168 25 20 = " st oanm02 17s 267 29 o 8 Ed own902 167 7 208 2 n " oson2 163 257 an na oso 177 254 2s 0 " 57 osm202 179 269 26 os7 osu 171 255 23 22a BOsI man. 165 25L 6 L 210 2 n " * nPtAiRca-teraoyssosnwyhimchbceAcgsovceRwaaasliwslaoyrnd TigMohlteIsnPeAnRs'iy 185 186 160 155 149 143 143 14s 148 148 149 140 143 166 155 145 161 142 151 145 161 145 187 180 134 3138 11 128 m2 156 136 138 150 17 143. 002272 -81- _---- PROJECT NO 454110 (Acpopnetnidnuicxd)5 TDT ue Minimum BimtiniT EnMveimronmentaMliCronadRnitieonMseimdy6Mem owwosn ma73 amsss aaes wnoonmsos woooem mw2s wmo2 uosa aaw8 aa wooome mm3s w as maas aa u ow aa owmemr moss oamsee mass anx oaw aa owumn 6ss7 2m2 mmsa n 5 swouow oonsn; 11677 wwee mues 6FEwE s womwe 2137 smzr amss a0 onwose wwaie mwees aasses aaua vnooe oomw wwaaned >me oasw aas nFsEwE oa wwaovwe:r 1a7 aae4 mase 25 onw oma wwoazs 110s mxss mmos 8swmowow woame 63 mmse mmaa a wuonn wwoaoeo mo1s aamwe mmse a wu v nou wwoome: 2a10 mmse aas 5" oww omow wwooen 12 ams2 m0s Fs E oma wwoosr mms2 0wsr 2ms a5 aow a TT AheFsaepomwmebeneogsemmhwaaabeed T MeesE pAR u1ss 116515 w1z6 nnss w1os u1ss 11605 n1e 117s 3n1 11ss 1ism 1145s3 n1s2 11s3 1w1r 002273 a- _-- -- PROJECTNO: 454-110 Appendix6.1 Alfalfa Emergence. Treatment Naber of eredSecilingDsianyR?eplicate CGornosuopl A7 i5o ioc D3> 7- M5e5m sul1D0ev. 9eimmgaakineg 98 5o 75 55 i` 782s5 01xs0 @Bsommeganiiktsg 86 56 75 85 i) 070 T1as lowmgaing 0 o s s b 10 2m Days Grreosuepnt NuAmberofEm5ergedSeoncginReplaDt: n Mean siaDev oiCmongtaoilng 83 15 05 1smenife 9 5 5 53 s 7` 85000 o11s5 : Fr @SSommegraiinngg 97 57 7 55 ` Z8N0 Lis 00mg 0 0 5 s ` 20 aw "Treatment NumofbEmeergred SeedlingsDaiyn2Re1plicate CGornoswopl A ibo ic Ds> `. MSe00n su1D1e5r II9eimmagaiinngg 98 59 35 s3 :: 0850 orsas @2sommggaaiikngg 97 57 79 :5 ` 78s0 a1ms 100mening 0 1 s 7 ` 32s 3m 002274 _83- -_-- Wide Le Appentix2 Mean Alfalfa EmergoenDnacy2e1 PROECTNO: 454110 u oder fo{ b~~--. ~~ i. ---- ----. -~ 2 T-- . 0 mm wm ow TT 2s Concent(rmagta.i1/okgn) wo ConfidenceLimit CarveParameters To ConfidenceLimit & --o--aregen [7 vaemmmat,en me 2 --- TE m em wm r wm em em =~ ConfidenceLimit ConfidenceLimit : = 002275 e-- e ---------------------------------- Ww. PROJECTNO:454.110 TGoorm sLioCmomniaelne lGMoosommmaaiinrngeg Appendix 63 Alfalfa21-DaySurvival Naberof igSling Di ayph A 5 y5 85 iEo s bs s 75 15 4 `'5 5` 7 01 : : f Osesnes suoopxme O of essm se anaam an 0 AG 9 ETN, T Tol e TNNU i NC %i a NC 2: ~~ a o om ww we we Je------ -- oa | -- Regression +veeee- 905%Cobnittinen. ] me --Cu Tam --_-- e=m mam ConfidenceLimit ow ConfidenceLimit Carve Parameters mw wm ConfidenceLimit oudm ew' e amam om ConfidenceLimit 5 ame . 002276 _--m w PROJECTNO.: 454-110 CGornotuopl 3Igsiemmggaaiisfgg @2s0mmggaaiiflgg 00mgaifty Append6i.x4 AlfalfaMeanSeedFrelshiWenighgt,Day21 x T 5 T < 5 n 0o1n0 001100 0oiz6 000D9 `7 O00U8 0001 000 oonnl `` 00-3 0001 o00w3 0o0o3 31 Me-- n suDev, o01n2 o00o1n3 oolo o00o0 00m2 o00w0ss az ox} ERAN ;Fos,. i a ee 00s Se a01 0 ww ww : me Concent(rmagtaiko)n _ |----ReDguraession errr 95%ContInt. 0% nbbion] me CarveParameters T hi Coe na tecmins. Conetewcis = I) nen we aren - ? own aston E --_-- hiE d EcCoonttneceiuC msw OCeonsdteteit hdE C: T C ` Ton Tam ma her oom asus _86- 002277 _--mm, Wildlife Led PRONJO:E45C4-1T10 Appendix 65 AlfalfaSeeHdeiglhtoin Dnayg21 E "ome Replicate Height (cy m) forPlantNumber: no----Me--m SO 33 4s 6Tw 5 Ww Control BA.53646.8634.61726554 98 6s1z 2oae 5 C .7 48 0s866160085 866s 810810 16 21mm 391mag BA bc 705s1s0048768596 s7 14 98 6110 2i2m 69064 6s7s 4734 84 88 565 aisms Isemgaikg B A 05S888755s s4 s40559434 99 3si5 iuem bc S22L5678735775 54s 86 4652 o2mm Qsmaikg AB. . 5665s58577451602. 71 6s6 2o6m bCl 6 85435 68 41057 7496 26 99 7si0 a1% 2omgaing B Al . 34E 23 24232223 48 2300 oowm bc D>l. 44P2432335224 76 2352 o1wa 100mgaing BA Teo e : i STe in we Ti yma dictsbcet LTa L i; ii 1P4ow10 seeingee8 ntErgo 5drr TASTE om 87. 002278 0 serimon em . ITo -- i. ~~... F M m0 ts SE 0 ey ars mrt Ce - ----Regrssion evo 905% CtaobnitIn.t. -- _ _------ Bo ConfidenceLimit ConfidenceLimit & : ' = ConfidenceLimit ConfidenceLimit x 002279 _--_--mm W. PROJECTNO: 454-110 Appendix6.7 _T--ry_cmo--en_t--_R--eplicate AlfalfaSeedling Condition,Day 21 (Condition (scor.ign)'9forPlant Number: TT 3 ase 7swo "Man SSSa Control 3simgaing BA a0S . e 0- cSON O0o co ec o- oo 00.. 0 000. 0 00.. 0. 00.. oo 00.. 0. 00.. 0. #&0 10 105 0 3070 00 5 o-oo oo oc oo oo oo 4 0 00 BA c 0. 00. 00:. 00:. 00. 00 00. 00. 00.. 9& 00-0 0c or oo 0. 0 0 0 9 00 0000 1 m3 > 0. 0. 0: 00 oo 0 0 0. & 0 00 is6mgaing BA 00.-00.. 00.. 00.- 00.-00-0.0. S0N 00.. 99 100 30000 5c ON ON 00c 00c. 000 00-00 -0oo 86 103 2005 Gsmgaing BA >c 20mgaing BA bc . 0-00: M0N o0o 0oo o0o 00. .0. 77 40 1030 001. 00. 00 o0o. o0o o0o o 0 o0. 00. 5 00 O0000 Loe ee 0 0 00 00. -00. 00. -00.-59 210 Mn73 : 0. 00.. B0O.N o0o. 00.: o0o- o0o -00-78 21%3 3M534 W0ngaing BA : . : Lo6. e1 0 5c 16Lo 0-160-100. 10 S10N0-1G00N. 100.0- SGNN 75 72 d1s7o9 TT TNih=neNdeiccrsaoydtsmebseaosld isnedwiciantge.sItnhtaetrtmhedsicadtleisncgorGedsaoretaesmseirggneed.foAsicnodriecaotfheinrdeilcaatitveesaeoverraylosfeoebdsienrgv.ewdhiile sog cforKeEnoYf .100 0 002280 --_--m Ww, PROJECT NO.: 454-110 Appendix 7.1 Flax Emergence Day? TGreraomuepnt NA oomfEmbBergeedSered[li3ngsinReplicatDe: n Si= min 77 59 7s 77 `7 2iSsemmggaaiikisg 98 95 97 73 `` 12000mmggaaiikkgg 08 o9 oH 07 `` Day1s "GTrreoautmpent __NA uomfEmbBergeedSreedli<ngsinRepliDca n 3"9Comngmaoling 77 55 3s 87 `7 @isSemmggaaiikkgg 98 95 97 78 `` 12000mmggaaiikkgg 08 o9 05 07 P]` Day21 "GTrreaotmwent __NA umofbEmBeergred SecA[ii3ngsinRepliDes n 3"oCiomntgraoling 7& s9 s8 87 `7 QiS5emmggaaiinkgs 98 97 78 `` i2000m0gmgaaiikkgg 08 o 0s 07 ]` Mean Sul Dev. 672550 110206 855000 [1940 072050 01070 Mess Su Dev. 772050 114216 885000 018020 070205 o17o1 M7e2a5n Su26Dev. 870205 01520 782550 a100 000 000 002281 -90- - nnn oC: see Appendix 7.2 o Lp] F-L--] A 1 1 E 1 1 Em I | -- EEE EE. & MSS re ISAC IGN ream T een itv y foen) Pet 00 a ee dl Conues nm ves0 wns _ ee s en Hl ma e EC andECy esumatescokouatedby knsarinterpolationusing the IGPINprogram(7) 002282 -_ IAppendix7.3 aw | 3 | `NEN 5., EE ./ I | ARE oI 2 FoS F WSf oFSS oF Ste EC Cven 2258929 crmmeim 150.6256. comesin 367.5893 002283 ----------------------------ereeeeetererreesseen PROJECTNO: 456-110 Appendix 74 FlexMeanSeedlingFresh Weigh,Day21 B-- EGone -- yS-- r-- r 5 -- no Mem saper sSseCimomgmusaiilnkgs o002m61 o00l1Bs a0Ol1Be aoommn 44yomoomm oooooimns fGSrSoymmeaeirrnsg on ooom aomu oo:mn 034 oomw oooomn as and ?gost \}. zFool t\y| Eos 3 Ee om = ne . aos Concentrmagt1io)n on1 --eoReCsonet | 4 bition] Te hoirc) TTT EC Tr ee aon ConfidenceLint ao ConfidenceLimit Curve Parameters ee rt ConfidenceLimit or&) emse ConfidenceLimit wa: ~ -- o2w 53 002284 Wildl I Lid PROJECT NO: 454-110 Appendix7.5 FlaxSeeHdeiglhtoinDnayg21 TemCroeup, p ReplicateT h`Heeiigghhtt((comm)ffoorrP!lantNumber: mA n Mean pl 1 2 3 45 6 7 8 9 0 Control A L059 9 9 8 1010 7 86 12 Bc 5s 9. 9 19 11128109119255 96 9818 222632 oD. 181M 7 009 9 7 91 135 39imgaing A . Bo. 8 8L19019 910109 1100 75 9936 009359 c 8116 8 1 U1 9 3 81 340 D 783 8109 9 7 77 229 1s6mgaikg A. . 9 10 8 1110 9 11 10 8 98 1.04 cB oo.91089 98 18111196995 92 97 a8l0 039136 DL. . 68 8109 7 8 10 8 83 139 625mgaikg A B . 5 i8z 190 110098 150 111397 81 99 8868 317808 cD 9. 8 98 31171039 59160710 97 77178 226346 20mgaikg AB .o co D . CCo2.1..11141103 050 S111 1 410 000 Loo1 22 2 4 a8 050 00mgaikg A . . Lo B . 0 c D o.LL . . ME __0 The symbol dicate tha heseedling ber 10nokemergeof ed rortomeasurement. 002285 -94- --er--r ------------------------m-------------------------- Ww PROJECTNO 454-110 e pym slssCeimomganaininngg GIZoioommomaagiiairrngeg Appendix 76 Flux MeanSeeding HeightonDey 21 y-- mE EmEeEE 5 n 58583 6ST5516 8&810a o2r1 8 74 ` slso a1s3 9io 8m1:s o4 MS8esm suoopmmer 8n2 osooms 0 8 2X fg, 2, 4 2 ee " e Concentr(magatii/kogn) TReogroesasion eRCont 0%Inhibition CurveParameters -- ee koi n Condperns im. Contdwemieeeinit e>n on r'= T T a E em E EEE Se ConfidenceLimit ConfidenceLimit : C 5 002286 Wildl Led PROJECTNO; 454-110 Appendi7x.7 Flax SeedlingCondition,Day21 TEoEr --_-- mRemer T TComny v E teimn eee re es fy w rPl w Nors t m e "Men 7 Cont AI Ble. d 5 ewCooeweoooweoawoo0ooo0w aak "owe Eww p 57 o8o w8a 88 simaine A8 5 Wee LGO0E 0 6 oAe o owE a ie w0o Eoawo o0 345bbon omom Semaine A<5 5 Goeeoooeeo o o ow o e ooo ow a 0oo o0 miaceo74 oo0no a8ow oo Wey ow Qsrering cA5 5 oeE ee o oE oon ool ooo wo EeEn oo EE oaE2&E330E 8B8 ow x Ew somins 8A 5 We : 0Im o0l We d0e we mW0ele 00 MWdUUoeSC WC Mm0iUs.Sc Roc Bmwuscme MC mwsoueus BSC 55& Bkmtss0s 7B omnening A . i" 5<8 . 0 o0 TUiSRCn-UsndyimhdbeeiodalSdseteededdiCinenoegs.IhLtCeeLeesnteCgusrorNAes-naNekescrioegrnee.d 0Asdeirasoheinedaicta sseverreiatyloefeblirngyWHignRoFfStyST.OO -96- 002287 _--mnmmm--m--m-- PROJECTNO.: 454-110 Treatment Group `Control 391 mgaikg 156mgaifkg 25mgaifkg 250mgaifkg 1000mgaiskg Appendix 8.1 Lettuce Emergence Day7 `ANumberof EmBerged Seedli<ngs in ReplicatDe: 9 0 9 & 9 9 10 10 7 8 10 9 7 8 10 10 7 7 8 3 0 i 1 n Mean Su Dev. ` 875 050 ` 9.00 os ` 875 150 ` 850 129 ` 8.00 141 ` 1.25 1.26 Days Treatment `Numberof EmergedSeedlingsinReplicate: Group A B c D n Mesn Sud Dev. `Control 9 3 391mgaikg 8 9 9 5 9 10 ` 875 050 ` 9.00 082 156mgaikg 10 8 625mgaikg 9 7 8 10 8 10 ` 9.00 115 ` 850 129 250mgaikg 10 7 1000mgaifkg 3 0 7 1 8 1 ` 800 141 4 1.25 1.26 "Treament Group 3S`iComngtraoil kg 156mgaikg 25Smgaikg 250mgaikg 1000mgaikg Day21 "NumberofEmerged SeedilniRepnligcaste A B c D 98 3 5 9 5 5 10 10 8 8 10 9 7 8 10 10 8 7 8 3 0 1 1 n Mean Sul Dev. 4 ` 375 9.00 050 082 ` 9.00 11s ` 8.50 129 ` 825 126 4 125 126 002288 .97- --_---- -------- Ww. PROIECTNO; 454.110 Append8i.x2 MenLetuce EmergaennDyce21 10 cman, F i. IN NC i oN da 2 . om Te oe SNS. mw wm we CD oaotmi eC, btm me Comatacnsrins T m e ee TT = mm ConfidenceLimit se ConfidenceLimit w%m e ee? TT TT Ee m ee , -- Cu ConfidenceLimit ConfidenceLimit x c : he 002289 - OOOO PROIECTNO: 454110 Tr --iHmeemia mSSoeimamln Appendix 8.3 Letuce21.Day Survival Tambor SipDuy + Mm supe B 8v+ 25 433 h m m4 4maommmoimm 3 814%8 4 e3mm aa= " ol FN i Ne, 2. NG, : ze ~~] omc. 50% Inhibition mm ww we me Coocentr(magt&1i./okgn) [A --_-- ~~ id Confidence Lt Contin iit e~-- : T:T er-- ete meeeTM 5 Confidence Limit ConfidenceLimit c 99 00032%290 ------------------------------------------ Ww, PROJECTNO. 454-110 Append8i.x4 LetsceMean SeedlingFreshWeigh,Day21 -- Geo aTv F E3 Q 5 E a E Men e sepen sloeClmomawsiilkdyg 00022860oo3nm 0oa3m; o00m%x 444 oooxsm ooosxms ioaZosoommmsagaiiikddsss 00000006 o0m0 0Oo; ooGmw0 442 ooooom oooomm or as 53"00 Fol Ef&EH oeegcusswio] n |-- 905k%CaohnbtiInotn. oor \ te ee ) 0 mw wo wo dow mw Concent(rnagtaikoen) Carve Parameters T w a ee en ECn ConfidenceLimit ConfidenceLimit 5 . hd ry 3m as -- em oem = Contesimi Con= tes iis = Fr Ir) ET) TM 2 wn wo 0 002291 ------------ eee -- S-- re -- e-- PROJECTNO: 454-110 Appendix 8.5 Lettuce Seedling Height on Day 21 BE Con Replicate --Heigh-- t (cm)for lant Number: n w Mem s pt T734657 8 5wo Control BA.0 6 . 7 388996856485768 s 98 16 1I2m7 cb li6s78486s66661055 76 99 6593 01m% 39imgaikg BA. S. s6 65 75S 74 74 55 47 45 98 5s6i 0im9 cD7 64 6433746633 433754 09 S4O2 i1em IS6mgaikg AB 4 5S 46 47 34 56 35 56 38 120 08 6316 11086 DC 6. 4. 45 763664 45s578s2 J8o 4575 11s smgaing BAo.. 5 5S 32 22 22 3232225s2 97 2287 1i3u0 D c 23254105121223232 22 180 230 0i8s 20mgaing A B .l . 1 2 1111 11 11 11 11 &6 1110 0030% cbo ll iTr1r 1a 167 1100 0o0w 00mgaig A Bo.... Lo FE] [EET] : cDb. : : . SLlie1 10 Te ym dice hteseen cir 4 otcng ofGdprior resent "101 002292 --- PROJECT NOL: 454-110 EE Gooey-- soCwomiong @ZioSsmemgmaasiiinndsgs Wma Appendix 8.6 Lettuce MeanSeedingHeightonDay21 iT se ro 5 T5T1 a1 s3e 6s s 4525 e4s7o 2L8I 10 2170 3d:0o 2d0o i -- Mem--su-- pe e 44 o 55000 notso 434 If1o oooxmo 1 wo oh 7E ER6f8 EO+N Te rors ---- RDeugaresion I . Joeconst havin 204 i 00 W000RTT 00 120 Concentr(magatii./okgn) Curve Parameters e--r ----------T--o ----------T--on--e ---------------------------- N --_-- i= cCoonfimwdeaneceuLimwit cCoonfmiedmenoceesLnimiC t w%o Ti core s-- e ---- To----T-- r e-- ee--mmr------ -- -- -- o E= hrid) n ConfisdeoncmeLimit e ConfiodenmceLimis t M pr = 0 i 0 Thdi e ao 3 102- 002293 -------------------------------------- Woildite Ld BROJECTNO:454-110 Appendix8.7 Lets Secling Condition,Day 21 TET E = Cmomes e iSe Comos eis rr be: Parmtesss PE Gam 528A eoCo w ooBow one0ewo0r dwE0 EoroE oEoLy orwooy oowvorof5 oorb0 oaw [ETRONENB:Booe ew[ owdeoaeow oobA dreor ro0e Eo0e osaw oseesosA r0 o0w on B2: oe oe CoGw oowooosRRe oooNrw oE ry oroE y oorw S ox x hrsoorI onu amin DFA:H oan wn wsr Cno oamEnE nooSBnhYORaMMPn EEBENYVRBBE YVonE OByRVY EOOomBm 5IE onE oOFE EoOoWRw domids 5:iA wT e o wWoN mda0nmooaS ShN oRmN6eRaavN BeE0vN oMmeaven SSe6ho r003 yB3wOaEpoMmm [EAST wo me mx sw ous Tio s rnodtc5uscd0s5e e0eadiCn:gh.Iee nSmoCeoti cr5s en rogroTnrilTlSa:.itsvTerTyoSfhsheA1se0of-1|yT1O0O 0 "103 002294 - 00 Woldite ternationalled PROECTNO:eio OnAipopneEnmdeirxg9e.n1ce i T re EoE ur ETS e FoBmeremanicsm 0SYoAy3HA33 S S31 E p4 A momom MGaommii 3 8 4545854 wmm om TEETe Duis Rais ew sane -- Foerfmiteaee nrrS b SE AwSJr-- S 3 --S-- E5 EA-- S1E -- iJMsm s2pe mSmaalm 25 v 6 fa 44p4 mam aeas T Se RENpuE nE wm mom simese ow 5 5 4m i FGSeiememelars S2H A4Y Ef 5 4fiAm im m oe. 00.2295 C-- o ---------------------------------- Ww, PRONJO:E45C4-1T10 Appendix 92 MeanOnion EmergoenDnacy2e1 n F jm E 7 is HEN. . Ri | | | FSS Ss ST& & & & oF eT TreatmentGroup "Treatment group mean significantly diferent fom the contol msn (Dunnett's st, p00). he monic ersion eho le fogenrewelres. Trnarepolo ationw, swed Ta StsRls Ea Concentration me Confidence Limit ome ConfidenceLimit Ss 50.7750 121515 194.5350 EC 0 30 EC 2c0e8.t3t33e3cladby2l5.0u000ton sing a C64P4.N23r0o8gram 1) 002296 -105- --e--e --e----------------------iemter---- / PROJECTNO: 454-110 Appendix 93 Onion 21-DaySurvival TGrroovwe A ambp erovifg Se<DlIanyR2le1psl. 2 SSieCmomngtaarioilnneg 170 1570 5`5 i`8 `i7 sSommaiang 3 Io o3 30 i` ioomaing 0 o 0 i MTem ISDupe 0S00 oLims 5a308 ooswm ow ow 10 100 3- 80 $ ioe eoow 220 ! 00 io . 20 li Concent(rnagatkieo)n [=rerome ----Regression Ip---- | -- so ahbiton] CurveParameters T m a er n rhrid aomn ConfidenceLimit saan ConfidenceLimit wo*m we: ons TThii Coentemiis Conetomsiri a x Sn a wns vom :~ : oe ons ~106- 002297 --rr---------------------------------- Ww, PROJECTNO: 454-110 Append9i.x4 Onion MeanSeedlingFreshWeigh,Day21 a poy 5 5 ------2 ----Mem supe sIseimmaaiikngg 000066 0006 oamm 0o0l A + ooI mm Toooo @SSmmgaaiinkge 00 00s 0 ow loony aig : 0+ om oo: m :: ass ox, 3 |i HFE oRAusEYii Eri om f\ nl wo \ ~ ~~ SS om ww wm Conentrmagtthoe)n 5 --|r --RoeBmguraecsoinotnne S04 Inhibition aw CurveParameters = = Contesiri Conteciris * : ; -- oem we ew wm owe Ta Ta5m oem ConfidenceLimit em a wn ConfidenceLimit w% m em : o2me 002298 "107- _-- Ww, ee PROJECTNO: 454-110 Appendix9.5 Onion Seedling Height on Day 21 ThemGroeup.n p ReplicatT e Heioghwt(cem)forPlantNumber:er nmn eMm ean SSAd T7345 678 5 0 Control BA o. LB 488779 89 75 81388 87 1866 0382 DC 72.7717078 897110130s1488 2 09 7864 320600 9imgaikg B A 8786 97 56 77 8887 87 77 75 1100 76115 o1.0m8 oCbc . . 8989998 71.7877 88 88 1193 2080 1s6mgaikg BA. 23 8568 586u7s 88 69 79 1143 224p0 Dc S981.4576 98 81 38 97 48 85 71 23800 @Smgaig BA bc Co 45322 14 3 30 0 10 Im Cl 3T01102s033 30 13 dn os 2omgaing ABl : . : FE J][I . ocb : ol l : le0 : 1000mgaikg ABl :. Co - 2 00 . Dc r: te . oC : 0 . The symbol nici hath seeding ee G0 no merge of Ge por 1 srr. 002299 -108- _-- nn Ww, L PROTECTNO 454-110 Appendix 96 Onion MeanSeedlingHeightonDay21 -- I GCrooowl -- 8Z 6 -- rp y 76 c -- S5 7o -- Mae1m su0pse s@eoSmimamasiiindngsg 7647 30 71s3 20 1015 171133 :: i 33somooums 2i5o00mmggaaiifnyg !: : 2 ; : "os sii T< og = ---- RDegeression iFob te 1 5m5%aCotntolnn. af aww ww wo ido B Concentrnagtikoen) Curve Parameters -- Tere --_-- S5 o eConfeiaderncsteLimit cConfe isdecncieLpimit N To c o:s 7 nCn -- e-- e-- Tm------ Tre ------------ 2 a= on oCownfiesdeancsezLimit eConfiw2denmceeLimsit M Toxs C wa n am: o "109- 002300 ys L PROJECTNO.: 454-110 Appendix 57 OnionSeedlingCondition,Day21 TE een Contin Geren) Pr ve = oor S SSS SS ---- Cot 23A 6 6 ooo oCoRo6o owow owoe oowoo w w0 oo0ews2 0 db mo8ew semaine FAPoRoEk0 A0oC A0RSeTa AoEOoNA AooT ooN Aoow o Aha os4 uWws omits 52A GeoWC@eoobwwowwoowbwo owww oowbowooo ooooo sP Bobm omwms 5 2Bole C CWowEwoTnwowrow Eoww owaowwew ow ooaooos oovouas smite eA3 we wa wToo w" o m ddoeo ew0e o6onn oo0n ooonl Q65o %@biws semaine A55 w:Cow den w dE e wda e wfomo wfoeo 54 dMoe o0o [ro 25 we :Coo Wow 3oPiedo 6 325 Co : : ooo l iffo seTco ,T merce h rB e gr d Bo c Bhaetess me) fne ERoETO o- 002301 w Lid PROJECTNO:: 454-110 Appendix 10.1 RyerassEmergence T veeTGrreaotumSpenet c NuAmberofEm5ergedSeedlincgsine ReplicatDTee,eeenemeMreaen es--uD--ev-- siCmognaoirng 97 55 7s 7 7 78705 01%0 @issemmegaaiinfgg 78 7` 05 `s `` 7i50 oiss 0M0ommgraiikfss 6 + 30 30 `` oswo oLma Tresmme NuomfEmbergeedSroclngDsaiyn1Resplies GCroonuopr As 55 c7 D> 7n MTeOm suoDmev 3Ioimmgaaiing 99 105 1100 5: bl` s 025 om0% @Ssommgaaiitkss 67 00mg sing 1 ss o o7 55 o `` 5812ss 01%2 ` 0s ox Tresmment Numofbmeergred SeclingDsaiyn2R1eps: Group A 5 Contr s 5 c 7 D3 7 MTem oSm uDev. oeimmegaaiinngg 59 15 I1o :5 p 0s2o5 o0m% @osmmggasiiknsg 67 45 79 1s0 `` 58510 1136 100mgnifs 1 1 1 + ars oso 002302 m- Appendin102 MeanRycgras EmergoenDnayc2e1 PROECTNO 454-110 a 0d 314 , Fool .NL - iE. O=N -- 2. om Tee ---- ---- -- ww mw ow Concentrmagt34i7okn) Toe] |----oResgreCssaionnt|| ok avbison me Tore Tmam hid ConfidenceLimit Tehiwd "Tonos ConfidenceLimit CarveParameters Tr Tw ConfidenceLimit wm w Tew om ConfidenceLimit --- owCe o m ' 002303 ne Ww PROJECTNO 454-110 To E WeCmoe ane fGSooymrmeeatsA Append 103 -- A amee roSelrigs DEayK2atelg, Mem sabe 5 5 >: ds T I oSnesoonom 7 82 ;5 ; :; o V Tasmvaomm u 10 FNU N i ON, i , See pTeoien)| ac S04 hin 2: ~~ EE -- TT ow me wm we ow Concent(rmagta.i1.okgn) Curve Parameters r a Tr E -- Scr ConfidenceLimit ConfidenceLimit hic . ? T wm T Tm , ---- ECn Confidence Limit ConfidenceLimit % . ' Sa 002304 Ww, Lid PROJECTNO: 454-110 Goromupe tmCognaaoilng @isSemmgsaiiffkgg 0M0ommagaaiiffgs Appendix 104 Ryegrass MeanSeedlingFreshWeight,Day 21 x Me W5eg@brcRete > n 009 012 R 0; Ton 7` 000077 000067 0O10 000089 `` 000013 00:024 oo.l oolo 3` Mem SdDev. oonn ooao 000078 oooomn 0oocl oooon 030 02s om fi Sault 2Poo fookd . eSe een Eos . a . F hao0 ee Concent(rmagta1i/kogn) CurveParameters hd CTotoesotr ConUtpepceirt. hi 7s0sss eons = a Bo CToonmteec it CoTnrep.ins Lu wn I) on ona nas aa oe oRewgrCeosnRetgreisionn. | | 0% ion] : : zon ase : Lae cums 002305 ------,---- PROJECTNO: 454-110 Appendix 10.5 RycgrassSeedlingHeightonDay 21 TemCoen Rmepns mHeieghtc(cm) for Plant--Numb--er:-- no T4 T 73 s 6 Tw sw Coral AB 15 105020150240 216 1167111 22019 Ls9 cDL 1 i8178 119125 214 019 1169 1138 &7 3Simgaikg A B1.8 1371718210S 1168 21221081165217 162 J9o DCo1.816L2916151164 164 21011531157 119 I8o ISemgaikg BA o.12 113 11B59121D 82 192 414 917 5 99 bC 19 141M613320913289M1U2180 @Smgaing A cB D LLoM.99 6se10 11831UsIs18 127 87 0S0212101906 B4 B15 176 01s8 1155 99 20mgaifg A B. L b cll l L3 405 6 4055 34 26 2642202132432 52 67 00mgaing ABo . . la2 a1 cD__._. . . "a. _---: 2 01 The symbol dicatetht heseedling ier 1 htcrgeor dedprio to meaner. Man sSa O e16s3 a3ym 117695 23434 11523 423645 1la4ds 24460 B12a8 2sn9 113486 3344 1B3L0 aasme 13540 4m 38 4ass o10ms 3202 1anB 2a0 .: 20 :" 002306 -us- --_-- PROJECT NO.: 454-110 Gorom slosCiemmoaamiinngg QSSommgaiikkgy i0maing Appendin 106 RyegrassMeanSeedlingHeight onDay 21 x m5 mCm c 3) A 111346816m1382 BMT4s6916MM34e 444 B45A 20 B45O 30 i3s00 B27a 20 145 M6a5n su0pe m514 o1m [ 30 EToomw 0 sh 2 SFu8h0 i, 2 sh of 5 Mu-- > TS-- ee To a. Ra e Concentr(oagatiko)n ---- ReDgruession 99 Conta, 50% Inhibition 10 Curve Parameters FE F oind oucowton nton ccmowmeomnen:C exo wa:n ao` s -_-- Tome Teri kiodm nme ConfidenceLimit smn ConfidenceLimit ehwd oo n C ais 1 002307 _--mm----,,------------ Wildlife Led PROJECTNO.: 454-110 Appendix 10.7 RycgrassSeedlingConditiDaoyn2,1 oy Replicate Condition (scor.sign) for Plant Number: a Men SO T wss ew wo Contra BA 0 001 00 o0o o0o o0o 00.. 00.. 0. 0. 95 00 000 5c 0. o0o o0o oolc o0 o.0 o0 o0 &7 00 0000 Simening ABo. 00 0o.o o 0. o0 o0 o.0. 00. 00. 00. W8o 00 0000 5co oe. o0o. oolo o0o o0o o0l. o0l. o0.o o0l J& 00 0000 is6mgaiky AB 0. 00.. 00. o0o o0o. 00. 00.. o0o 00 6.. 99 00 0000 5 Cc .0 or e0r e0l0 oooo oofo o0l oool o0l0 o0l 0& o0 0000 smite A 5 . 0-0.0 00.. 00 00.. 00.. 00.. 00.. &7 00 0000 Dc we. 60. 00 o0o oool oool. o 0. o0. 0oo0. I8o 10 3060 omgaing BA c5 OSN C0. dOlNe dWeONe O0N 00 46 s7m 5527 : DN Oo-l .sNN sNN N0. A0.N 00 67 21B 3l8a3s I0omgaing AB sn . Do . WoNN 1 1 %ow 5 c . : . :. : 6. 10 6 . TT TiNh-enNedcosisydmsebcaoldasedliticnaegt,e hItnthteseeerinsmcgore0edsdanoeikaenmseitrggeeed. 0Asionredofit0hceraelcaatttieevse asnoermvaleosferoebdisleitrnvgyeWdhisleiaosfgconic(sy100 002308 -u7- Wildlife loternational ltd TOECTN:eH Appendix 11.1 SoybeanEmergence a Ee - mum -- rt rrJ 2 2SEu A2 5S n A2SR = BDGelomavnae:e 228 52 B 22 BI 2 !ii B >om =oom M EE r puss B mf ewe n 2 e2 E0Ye: km2 enm,e7 E BGieamEE a2 SE2EFSO 20BWA S 20: E71 monnwTT. mB Em = oom Te E SenE etDE un a (LFf.ETSe 5 n2 AG FrEe] eA em$mu3i:mt W 52 W2 2 W B2 2k 2p :I 2 ne wo0moE B bawm Buim ns. 002309 nnn once.sir iis \ EEaEEE TEER R Hi | | | | 1111 J. & ed CF&CoFA& WA FCd F&oF& ree e--t--e-- e -- pa J. 002310 Ww, Lid PROJECTNOL: 454-110 TGrroeunpt siCmgoeming Comiaminag lSooovmmagaiindlge Appendix 113 Soybean 21:Day Survival Nbrof Ling SelligsDianyRzegn 0 1 y-- 09 11 0b1> 9 d 10 ibh5 15 d1b l 10 m10 2 ERE 2 MhSeonom suoopmme Gske oomm "vnte oomw Fl | | | | | | FPCCe A r GE o& e& oF& & & Trestment Group No veamer groupmeni gift fiero hecomenmDunse' sh,F205). Noepesionvasconducteddu 10th ck of eft 002311 10- PROJECTNO: 454-110 TGraomuep otCmosmions @IsSemmagaiikkss iZommgaaifgs Appendix 114 SoMyeab nSeeedlia ngFrneshWeight, Day21 x Meve5 Emb ec e 5 337457 34246353130a bi7 338%1 33 A3n% 4% hi` o23e6 021@ 01s8 2omm `` MTeem se0pmev. J3s 0oxm0e w20o0 o03m0 051 oos s SES ado"., ,7 . 22 , Ez .-~. Raa Se --_n, a 0 a 0 ww wo me ow Concent(rmagta4i/kogn) Curve Parameters Treo Tre ou as a ConfidenceLit am ConfidenceLimit x Ed Toner Teo id re ConfidenceLimit nase ConfidenceLimit Saser EZ Dua IS-- S ConIt. Lo s5o0%mIbnhbiibiitoinon| ido : c wes 2) 2 cases a) L121 002312 Ww, PRONTOE45C4-1T10 Appendix115 Soybean SeeHdeiglhtoinDnayg21 E Rept Tigehtcmm) eoro Pl Nor aeen = Bo Cou ABCo aNnimmmoa su nwwm om n omoowwnmow3b0o wmwmee se3mso D BBB moun wm 0 Bs Tm Simgaiss ABom3Ta%Hn Baw m nmoa wMmows MomMon aHmod0ovs mN nes n6sxi EEE EEE EERE ENE emai CABodoRumdamwt nwmmmoma yRw1usamsoa blooo wmlse iIamwm 5 Bu LBs n omn s me Iu Qsmaite ABMBm M3B33W3o3wd s o WBo0 mds anem CDR NumnanomusmmmnuRmowl0 N Bo s1 somgaing B A owmma a n mm u am ms moos mme anso cPD R22 m24 a17u18n2m0 2n0 m21 u16 22 n1s4 00 ue 19.4 3.10 oomgaihs BA 423103si3 64 483s za s i 3 owi a36 2od0o T GCDeo3di4s Fe3S 5 r4 ei 3sF4 sSAes Te 3aenc a 43 aomm -2- 002313 Ww, PROICTNO: 454110 Appendix 11.6 Soybean enSexlin Heighten Day 21 e -- iI mei W --E Bm -- s rew Be m Be a -- a 4 ma aEnT F SBeommr ianne oman mA d3s3 Bmwms w H mmis WmW se A4 W mmamon de =wy zEnb Es aTa ~. =,~ "ee, nSSe :5 ----. = mmCocee nregssw iikn) we "ow A seam S04 lahibition me e ------ ET -- e CarveParameters e hid ConfidenceLint ConfidenceLimit x -- TEE E _ EEr-- rE -- on ConfidenceLimit ConfidenceLimit % 2 : 4 se 002314 Witdlte I LLid PROJECTNO: 454-110 Appendix 11.7 `SoybeanSeedlingCondition, Day 21 TEEBE mRempu Conditioncmecr foP--h i N--orber: C aE e es v Cont Soimaids isemning @smaiss A Beoo eo Boo AB e o0. bd e ABew. 5 AB eoo0 5% 0o oe ae woo aa aoa oa 0o od a0 0Waw ro0ommmoeoo aaao oe dE woe Ew Shoo Ww 0o [A o0. m0ai.c 0o Eo E0 0o E o0 E 0a 0oO IiA03 ]0es oo a oo oo oo a o i 0 oo o e a o aea0ooa0 0aooo 0 a0ooa0 oa6n dm9 ooo0 oaooen oo ee oa eo as oo a 0o eaa l0 0o ooo 0 o0oo0oow0ie wo0ie oaof iomn 043 mb0s0s ow down NE owns 8 maim 5Adm.oir c oo 0.f aa 0. omoEolm0a oo0ol oo 0 noo0 mC0ioc wa0i. e 0gfoo 115 uN$ssE 5 8 ol ao oo oe oo om os 0 IOmgsifs 5CA OoHSNNC m0WNSSCCNUMMSINC MSOiCcNNh @WOsINCCN MOSSIOCNCN AOOlNNc WNGiNN WWoiILCCC wOUsNN J0Joo 44@ 117896) TTR sby_m_bo@liincd_ies hoaN seeMdiNngGoeknemerge.oAnSoeoIf0Cndiaoesnaporsalssclinge,nwheeasnre oif 00S uo "dNecirosics,dLeaaCds~tLeelaiefnCgu.rln,tSrCme-dSitnemesCcoorrles,UrSeCs-sUhge1dniSnedeeidcadCtoeatth,eCreLlatiCvheasreovneryof observed signs ofoxic. N 002315 124 ------------ e-- e -- ---- eee-- PROJECTNO: 454-110 Appendix 12.1 `Tomato Emergence sp Treament -- Number ofEmerged Seedlings in Replicate: re Group AB <D n Mean 3"9Ciomntgraoilng 56 67 ss 53 `7 665300 @isSemmggaaiifngg 86 42 6i 44 :` 555205 12000mmggaiafilngg 00 o3 02 0o <` 010205 Treament Day 1s __Nomber ofmerged Seedlings in Replicate Group A B < D n Mean 3"gCiomntgraoilng i9o s7 S7s 5s `7 890000 2i15Sm6gmagaiifkge 190 68 108 96 `+ 882255 120S00mmggaaiikkeg 36 08 08 07 `` 071255 Day21 TGrreoatumpe.nt NAumberofEmBerged SeecdilnRiepnlicgaDise: n Mes gCmognaoiltg 90 07 7S 100 ` 892255 @iSSemmgeaaiiings 10 65 190 96 `: 885205 12000m0gmagaiikfeg 36 31 o8 o7 ]` 712050 -- Su Dev. 19713 219919 o15o0 sdDev o11ms 1171 019560 St Dev. 019560 1i7n3 014316 002316 -125- Wilde Ld Appendix 122 MeanTomatoEmergoenDnacy 2e1 PROJENCOT 454-110 ome iPYY ne Eo B Se i Su Ha, 2 a een . -- 0 mw we mw wo Concent(rmagtali/okgn) Ta hEix) em EX ConfidenceLimit TTee ia ConfidenceLimit am nn CurveParameters em wn ConfidenceLimit Sas> ee ConfidenceLimit 5 aun wen [ocome] ----Regression | ee95%eCon. fInt, um wm ao? n hd 2 wm ams 002317 -126- ben maios mp I _ . 7 LX HEARN Regression o ses. o : eEe R eelie raw hid Contosimi Contes ni ~ e mo S 5 ConfidenceLimit ConfidenceLimit . ' ' a127- 002318 w Lid PROJECTNO: 454-110 Appendix 12.4 `Tomato Mean Seedling Fresh Weight, Day 21 MeGromm up vx Ve 5 Re>ER n EMewEsus Dev IiCmogoalifg 003&12 00B2 004% 004a0 7i 0o3i ooomm @IsSemmggaaiikksg 0005 00123 000%7 000x6 `i 00% ooims 0Wo0mmggaaiikfgg 003 : i0 on as os on asf 3 onl owl 2 and, i% omf\ Su- 010} WN me I000}SE == T a SE om Concentrat(inogn akg) "ow RegrSescsioonitn, [som aniion Curve Parameters m = m Comms int Com nes ins n : : wn ro) nem wo wo nm sc CoTnioa it SoCthroetins = on sao Taam I) : : a Sn -u8- 002319 - ee PROJECT NO: 454-110 Appendix 125 Tomato Seedling Height on Day 21 TCrom ment Reppliicate fHeeighet (ocmm)ffoorr PPalntant NumNbeehre:r mn Men pSSa TT 3a se Tw si Control A B s6 4626s434 27 545s67734 00 a523 L11Ss Dc 8647635646 0965 6655s 98 6sa 01:;2 Igimgaing AB 75674577658357 46 97 6540 11a D c 5. 6. 5l 85s 65s 37 5662s6 76 I7o 5536 112s I156mgaing ABo. 6. 733422 53 54s 24 34 57 98 a355 1Im cb 5: 4055354764758 77 96 5545 iae2 @smaike BA fl223S3323 2323 3034 86 2281 oomm eb . 3223332422322. 67 224 ooms 2omgaing BAo.. C.o b c o : L.2T2e2 20 ow >iL. D: 2010 do 100mgaikg ABl . . .: 00 . Dc __. Na . N Do0 : Toe symbol cies at seeding berG4nfrer 1didproio easement. 129+ 002320 Ww, L PROJECTNO. 454-110 Appendix 12.6 `Tomato MsnSeedingHeight nDay 21 eCoep EEpERE ECE Com SA eM Mseim su0p%e iGeiimmmaaaiiilnnsge 6a3s06 S33s 43saa 1ssss 44a3se ooonmm ITommuaifiy 20. noo:: B!m o:m u 0 of 5E4Rh iS [2J 3ENG 2 " De 2 200 Amn600S100 Concentr(magt&1i./okgn) TaeBo Toees ConfidenceLimit CarveParameters Twoe om ConfidenceLint ke wo cotimatins _catimertion ~ a on sans Tm ----Tteoguaasion reo Contlt, 50% Inhibitio|n 1200 em en : : en 10 002321 Ww, PROJECTNO: 454-110 Appendix 12.7 TomatoSeedlingCondition,Day21 hTadE ee S Coon a S be PerSs --" Th Get B :A S0-0oooo0 ow0 oe0 wo0. eo0 oo0 oo0 o0 b1F0oooo0 oowoom 5 CO Eww Ere a simenins 2sA o" o oo0 oa0 oo0l oo0 oo0 oo0 aa0 775 000 oo0oo0 5 6 6 6 a oo oo oo a io oo omaine S34 ow 6o"eohonaa0 oo0n noo0n we0rn e90x o0wow0 bs5 oowl0 obodoae 5 [A AS A A Gsmaii A23 0. ON Wo ON 0 AON wa NFoRnN GN oN C Ibno O S0ON EaSNNI 0 MN 150 B5I2 u29I47 5 WS WN aN BN oN XN HN ee 5 3 samira 3ASm o Wo wmne I0do I0doo lomoeob lw0moho W0lN0a TfeNeo &&6%Wwww 1o05o0s 5 COMO I ww ws wm 26 [A5 e 0Co :: " 0 j0o 3 0 10 oo ae 5 on i,a etleorBT an 10rBoeOR oe er of in ReeSBnreeoa,io fray 002322 REN nnn Romero: #56110 -- Blk hactmion Anse py, [= re iscosine mp SsomEonTe m J mam mm ma, Tram, En -m fooEEomTlmR--Em cue wm vm nsB#3n mE o --A--eE na aRS. a j--pigoninnitl ai" aess--nsn o Fralmiit fr 4 i LELEEEyyRroRgEen EiEonA s min&afi rSe ---- 002323 --_--_--mmm PROJECTNO.: 454-110 Append1i4x Resultsof WaterandSolPesticideScreening, We manLi.Wael ,ter: eseidOrsgs TT Teh Comes ewibC<eemnewm DiaCommer NNbeers wine Nec lode pr<ay <a DDiicctkioires Diciorren pNraen Aree <<aa DDDiiicocoketoporphmosei Anvapihcoereint = DDiiect.a BAseiapibocreint Bir p=r 0 DDDiiiopbahatnyiamcriniode BBriomamne Brorephosment pprrss DDuiroo n prs Endo! CaBproumtoo motsid tr pprry FErntiat! cSaonty Cutotrn pr<a) oEnnirinkeos CCamapvpeinnaion <<aa EEivtirni mGorsCoCthtirniene <<aa EEbvoepyn CClinrteinneeinom. <<aa FEerurdiapilos CahnoscCbehrseinoe ps prs FFeornmtes CChilooowoopocrsoppotapme pri@s feFFeeeplspaoinrn CCCohhooyorpanscmenits pprr FFlenoiarn y pr Felon CCoruinoecpurtes pprry HHeeppuacctiirrcoside GCoroazneer <<aa aHezsbeoHorrtersre CeSoy7m0re0e0nn t <<aa fictaeges <a Linde 5r70r00. <<aa Mtiamen p5ye-0e0E pr<a) NMemsiiudnsbes BBbrye-n0e0Tn a&&n MMietnhaoutrison Aaspertomedby sygenResch onape lect only 24,200 WegmeibG<ee%nmemn =<=n ==<u p=rs spprwry <<au aa a<"au ><a b=r:y =pry = pary pprl p&rs <pprrayy aa<x pprr pprrs p9r " 133 VUR324 PROJECT NO: 454-110 A(pcpoenntidniuxed1)4 ResultsofWatanedSoril Pesticide Screening Wie resoWenlW,at. PaAdirpcsPee) TedCorpo EE mborangi FrCeomepsonent NNNeeesataempration p<parr roPPrearlriee NJHeeieetvues pparr JJPeurneye NoMyvirnptin ZZoo joQuee]r NNoopwtpe e <5x0 SSRaonmmae!ires NOferuyizna SSiooo SSiSpunaetm fFrSieoernnte SSoo TToemiein &* Teron Farertiteemoentuin &&So TTfheinooyorde FFPrreoommeno pSprroe Tnhnootrma FFaFeroimptnunive ppSyyo TTTrainamnioan FFomopiioursient! p&r%y TVariyncoburn Feri x en Tinommnee Zmoonanny jToierye CrBCitcmn <ZZaooooss NTaNokeuabiem CCGooomurenern SpepHos SSSoeiernm Moarpes <3a py a -------- mo5prree s SSSooo p&5r pprye S%<on SS%oo %&So p&&%*r p0iro SpS&oor mZZooaoosml <Z<s5oos <"oooon0s Zo 002325 -134- PROJECTNO 454-110 Appendix 14 ResofuWaltetr a(sncodntSioniulePde)sticideScreening. Whe TT -- pA Nro .ine JANooowetaasswcont pANrraanormecsant BNeeranemen BBBriioromet CBoBraormtpobsotsmscent i ec CSCaohtraininCuotin CoCotrrpCriontmaisenieon CJiiicCuviatnnyoigion GSSimempoenpstuen Steheaprreite CSoiprtietsamit CaCGzarnnmaet Coepnooemoenarnt 5FS5oo0no0e0n Bpiet Denno HeesprtoredbyBygnReeeh aisiGrebSel. Pen00iOpss dbetrnany J <S=0o DDDiiisnionrn %&& DDDiieovrpemreh prSSsoo DDDiidiniesroon S=o DDeoniins &=& DD=iernyarie %S&o EExrrdioystitni!t p&=d EEESrtensa p%%t EEio=nin prp%sd jFEi demtes &aaoo FFTorarmons pp"yt PFFeeinnrnontn &Sioo JiFoaaneerr nie p=r&% f[rrurmeenes) ppprrrssy LLoirnpeeens ppr%ry NNMeaetsraeonrs p<ee) Njerteswaaesy % n rlscolec 24,20 VemECnm <SS0oo phhyoo Sihooo SSoo S&&o* &&So S&Soo SSoo SSSooo S=<o SSSooo S&aoo* Sp%ro SSSooe SSSooo pprs] -13s- 002326 Ww, PROJECTNO:: 454-110 Append1i4x (continued) Resultsof WaterandSoilPesticideScreening Wilenron], 1.Gross J-- EE omeregn) MMeetdaeor <<ax MMeetroitopanrin pr@y MceiroMieighos pprryy MMoinreaeronbos pprry MyLcbpant pprr NNopiropanmice pprry NOonruirnun pprry Onnfmheirtn pprr PPoinactxioonn p=r PPenodeamedcatneae pras pPerree y == FFhrosatisne pr=y PPaepntianidon Primi pray a PFPrrrinimmpteorreot a<&= ator pry Sl: PesAntdOircdsPeges2) Compan. fProomeyne PPrroovpaerlice PPorpohyatme PePrdoprona QQuuisoimenbicnse QRuoamkeiapetnt SSmiemeao SiTpreeteos Tfenrchyooriphor TTheincdwnocnuts TiTTtroetrnadsnole fTetl Tirdeensti fVTriecmeminicnubic Gaborng) <%x w = <<aa =<a =<n <<aa <au <u = aww 0prs pprrs pprr & AFninrieon CBeaiinn GCraeion. CCooonmver Mapes <5meal pSrent <p0ut <0107 <"aia<m1 ee Me= ry NMeokbhaihensm SFauensamo SSaidr Zoe "Ghoomera iLan <<u0 aSann <0136 tesprodby ypeRes onsrecollectedon ly24,2001. -136- R37?