Document zdEEqawLV1EErYJMqDwxDX50a

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassiumsalt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was notnoted. The test sample isFC-600 (fromAntwerp,Belgium).Currentinformatioinndicates itisa mixtureof 1.0% PFOS, 81.20% water,12.00% diethyleneglycol butylether,1.00% sodium octylsulfate2,.00% propane Sultonefoanier, 1.00% sodium decylsulfate0,.85% xanthan gum, 0.1% N-(3-chloroallyi) hexaminium chloride0,.80% starch,and 0.05 % tolyltdazole. The followingsummary applies to a mixture with incompletely charactetizedconcentration of impurities.Data may not accurately reflectthe inhibitionby the fluorachomical component of the test sample. METHOD Method: 3M Protocol,Reference EAR 11/8f79 Test type: Acute static GLP: No Year Completed: 1983 Analyticalmonitoring: Dissolvedoxygen concentrabons Statisticamlethods: Graphed Test organism source: Activatedsludgemixed liquorcollectefdromthe MetroWastewater TreatmentPlant,St.Paul,MN Test condition: Dilutionwater: Deionizedwater. Syntheticsewage: Not noted Reference and testsolutionpreparation:A stocksolutioonfthe testsubstance was createdby dilufin0g.60 granisoftestsample to 100 mL vathdilufiownater. Itisnotnoted exactlyhow the 1,000 mg/L testsolutionisprepared from thisstocksolution. Test vessels: Not noted Number of concentrations: 1 plus1 blankcontrol Temperature: Not noted Total suspended solidsand pH on day of testing:Not noted Element Basis: Respirationinhibitioans determined by oxygen consumption. RESULTS Nominal concentrations: Blank controland 1,000 mg/L Elementvalues: No acuteinhibiteofrfyecotn activatseldudge respiratiornateat 1000 mg/L vatha contacttime of4 minutes,45 seconds. Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The value reportedappliestothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS LimitedcontacttnieestimatesthatFC-600, Belgium isnotexpected to inhibitthe activitoyfactivatedsludge. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordof methodology used. The method was notan agency approved method. Test duration was not longenough todefinibveldyetermineiftheremight be other effectsthatmightoccur duringtypicawlastewater treatmentretention times. The sample puritywas notcharacterizedand the studylacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M EnvironmentalLaboratory,St Paul, MN, Lab Request number 8875, 1983. OTHER Last changed: 6/28/00 8 1f 7 E <5 CA: 4 lj@u 0 ui CL 0. -A oop, @,@-ol.".@ ENVIPONMENTAL LABORATORY SUMKARY SHEET TEST: Acute Toxicity of a Cmpound to Activated Sludge 141xed Liquor. SAMPLE DESCRIPT.: i3F-LCILI") ANALYST: DATE ANALYZED: .2 LAS REQ. NO.: 111 11 tiI I It H-1 llill1111111 j t I 00, N I t I-1 0', 2 F-I I ffl4 0 -'-T T-r-I 41 Lt TIME minutes r L4LL-L-L 3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to.-ActivatedSludge Mixed Liquor Protocol Reference: EAR 11/8/79 Lab request no.: Date: 2-loi Ana Iys t: IJ. 0 The activated sludge mixed Metro Wastewater Plant on a2.1c liqu r was collected from the St. Paul/Minneapolis Sample description: Fc-- GOO L- P,-v 1? -9 -7 -5'- 2 Preparationof stock solution: 6.(o0 grams of Sample yierediluted to 10<3 ml with deionized reagent water. Date of preparation: 0 TIME min. sec. 30 ONC NTtt ION O@O;EST KI;ERIAL N ACTI A DI ; CthT@m mg@l mg/l mq/1 lConMtiuol m 7,!@O SL GE gl)IBRE M4 1-7,3,3 ,2 16.2-0 3 3.40 00 70 ass=_- IL COMMENTS-.