Document zd8eQQb9br3OJRoY2YBOnQKK7

Wildlife International, Ltd. a. Priect Number454E-101 ADDENDUM PFOS: 'AN ACTIVATED SLUDGE, RESPIRATION INHIBITION TEST WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454E-101 3M LAB REQUEST NO. U2723 SerieUsS,$50EnviErcoonlmoengtiaal EPrfoteectiToenstAgGeundceylcs 'OPPTS py Number 850.1010 (OECD Guideline 202 his steuddy twashraernpaotasrtreetdviubsseeidrngoeni9se7sd1tb0s0uaabdsdtoatnhntehppeumurriottyyeowrfaecs9e0nd.te4tp5eurr%miitnTyaehnda1leysbsteess8u.6bTs9ht%ae.nrceeTsheusSponsor ested wer simply coreted by th tio of he wo puis or a aio of 09603. HomNo attempt waaslmmasde tho recasloccul,atieoanralyatriocrasl sdtuaendtoarrdouconndcienngtrmaatyiobns porres`emneta.surAetdaccohnecedntisrahtieons. revised summary page from the final report and the most recent certificateofanalysis. gzEgsn = 38 Wildlife International, Ltd. -2- Prsiest Number454E-101 REVISED STUDY ABSTRACT BASED ON A PURITY OF 86.9% Theeffetofthe estsubstanceonactivated sludgemicroorganisms wasassessedbythe i Evo acontol; refine and Heatment groups.The cht foup, was sed:to determine the. caa go]e8 18ghe Dcuaye a The1len si il sgmne yonwa0s.r9.1,025o,f07r,e2sp6i.r5at7i.oo2n0t, i nd TOT % 5 fe paid sre hi ionratesofthetestsolutions i were messi agai So ge ncter. The respiration ats ithetwocontrolswere i anil 401 br 0f3.2%. Thedifferenceincontrolrespiration ateswas | within RT al scfm established for thetest. Thevalidityofthetest wasfurther ii pet be ieh Sus fom he 3,5dichoropheno! referencegroup, whichresulted inan ECS0 | of9.0m. ITHEECS0 waswithinthe $10 30mg/Lrangeconsideredacceptableforthetest. Differencesin0;respiration ratesbetween thecontrol groupandtestsubstance treatments wereobserved aal est substan concentrations. Folislsuommawryoif henregsus Toss CoC$Souinrhoailo21ophenct 3mg 3,5-dichlorophenol15 mg/L 3P,5-edichrlorfophleSnuolluf5o0noimcgA/rcLi.do,PootassciumtSaalt(nPFOeS) 0.87mg ail P `Pele Puqor eecaf rne l fSSu Silulllffr oounsniio ccoAAAcco ciirddd,,,coPPPooot ttotaasasa sssciihuun mmtSSSaacallaltt(((PnPPFFFOOOeSSS))) 26mg 28..67mmggaa.ii/ll.. PPPeeerrfrflluuucoorooooccdkttiaenSeeSohSlsifocenic AAAcccii,dd,,PPPaoastssaiSSsoiallnst(a(iPPSlFFulPOnOFOSSmS))) 28$767m00gmmgEa.8Ai//IL1L. `mgGLb _ `RespirationRate a2n6 313 14.6 385.91 53B5.s1 232477 nko Percent. NNaA 29 638 83764 o1137.00 3310940 Wildlife International, Ltd. 3 CERTIFOI F ACNAALYTSIES Projet Number 4548-101 CoIaNtsTAEmtRyItcMaFtCrbE RooardvR sienTP1R0RI CO7O1Se0F0Oaf)iI F3r1A7C aNcAALY0T2S3IE 91S84 Relrncbee SB018 em [rey SE ENTE sow | [on Meals (ICES) 32.MtaSgooileusnmiem i PPotaassun' 7= [i oerhrdTair geieobo.in| fod: T1Fe S3 EeSEGeESAEEnE]| 21 000000iswwinkk 3 los SB seBEHEiEoRELT RtTL| gsaainoewa on sige rd 7 coooiwmn (cSmarsl) 2 RACKET TRE NoreB ([ S GC/MS,r raoa Jer TETPeTs IeEaE e]tRteREap ue [[R PuybySDe vseC (TOd R) |ad[eoSTs2NTGoT if gy8= T7T|DeeotxeAapplcicatt ed|| S3Thohew Dm [[8 SESrS E : Be EtCes 31 S saooos ows 55.. NPuhiotsspbme rFbagga a BBaSEEAR ER] 5C5 oooeonrswwnemmenn OrgTa nAcs 10) A a SLR oo 25otPrRmeAa k e[PBSEEEARITISSSEESUERCRSEETETRINNErOTn iHI 2 4 oewrne onus 73i fNceaanen F T[RE ohTeemAt mVeRe:IT 3 b |) beh h es i he memawmior | dua conan fa. Wildlife International, Ltd. a Project Number 454E-101 eInNeTAEnRaIyMaCELRaTIbFIoCaCAOTtERoeOfreFreAinNcsAe:LY(2S5I01S88 * DuLtasAonifs: W310 ------ SingsContos: Frozen10 Reasssoen Due: GRB101 P"Paurnitty,= 0SHABR pons Sri ad pire 0 SHAFOAR 100% - (sumofmetalimpurities, 1.45% +LC/MS impu8r.4i1%t+inoergasni,c: 033%) Toal opuritoyoom3llO es =e 11a3.05% Potassiumisexpectedinthissaltformandisthereforenotconsidered animpurity. Purityby DSCisgenerally notapplicabletomaterialsof low purity.Noendothermwas `obsfoer thrisvsameplde. "SnurlfopurisensnthootesnnatmhmpiSle0oa4pnpneearnstoso.belacTo.dnoversdtieidogtnpoocSnoOxia7nndsshen0wciieldevtaielcrteedeumsning,theBs STTPNFPETAAAA "HNPTeeeriwpafaamluoodorrrwooooappcneaeortciipccdmusoasdstiiaocdd "Theoreticalvaluscalculationsbasedon theempiricalformula, CoF15SOSK"(MW-=538) hsworkwscondoctedunderEPAGod borin Pris Sanda (4 CFR16). onmann J Wildlife International, Ltd. se Project Number4546-101 GeIaNrsTAEmRIbMaCELaRboTraItForIi CACOTAEROtFaAsNA:LYFS30IUSBA LC/MSPurity Profile: [CC tmpumy TwT wwew ea --Tw --1 eam ue I 7SE |SS Notei:aTnrhCep4na.nCd CT6avoaeldvCe5svowieern,waicicelodunueesitdngoih1eCi4h3oe0dwvCae5gselaTedoiarssubegscihroen po aor Gt 8as vs PowayDwias,ECoanesAnicaLsborsiries J Bw `ReviewedP By:mr T ------F So-. [E-- nats ildlife International, Ltd. COTOXICOLOGY & ANALYTICAL TESTING SERVICES December 1, 2000 Ms. SusanA. Beach. i En3vMirCoonrmpeonrtaatiloLnaboratory 3 935 Bush Ave St. Paul, Minnesota 55144 i Dear Susan: Enclosedpleasefindthe Addfoe rthenfolldowiu ngstm udy: 1. PFOS: AN ACTIVATED SLUDGE, RESPIRATION INHIBITION TEST `WILDLIFE INTERNATIONAL, LTD. PROJECT NO.: 454E-101 Ifyou have any questions, please let me know. Sincerely, ERDvI ahecch Edward C. Schacter `Manager, Biodegradation 8598 Commerce Drive- Eason,Merylind21601 Tel 0-822-8600 ix 410-822-0632 - Ema ecotoxgwidieinternaioralcom Eom OrBrgamoueg 1G7 LWa1rr TonNetTr1r057s5 T3F0oc3005875 STB1- Ema serovars