Document zd2DN2877EDxJQ4a65aqvpnE0

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh;i,ch may alsobe referredto as PFOS, FC-95, oras a component ofFC-206CE. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 502. The testsample isFC206CE (2/16/89).Currentinformatioinndicatesitisa mixtureof0.92% PFOS, 15% diethyleneglycolbutylether,76.09% water,4% urea,1.28% Sultonefoamer, 1.55% sodium octylsulfate1,.05% polyoxyethylene monooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% tolytriazole. The followingsummary appliesto a mixture with incompletely characteilzedconcentrations of impudties. Data may not accurately reflectthe toxicityof the fluorochemkal component of the testsample. METHOD: Method: OECD Method 203 and U.S.EPA Method EG-9 Type: Acute static-renewal GLP: No Year completed: 1989 Species: Pimephalespromelas Supplier:AquaticBio Systems,Inc.F,ortCollinsC,O Analyticalmonitoring: Temperature,pH, and DO Exposure period: 96-hours Statisticamlethods: Notgiven. Test fishage: Juveniles Length and weight: Average length 19.9 mm Average weight 0.06g (wet) Loading: 0.20g fish/L Pretreatment:None Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutionwater chemistry: Temperature: 20 *C DO: 9.6 mg/L pH: 8.0 Lighting: Not given. Stock and testsolution preparation: The testmaterialwas added directly tothe dilutiownater on a weighvvolume basis. Concentrations dosing rate: Daily Stabilityof the testchemical solutions:Not noted Exposure vessels: 4 L Pyrex glasscylindricatlankscontaining3 L of solufion. Number of replicates:2 Number offishperreplicate1:0 Number ofconcentrationosn:e plusa blankcontrol Waterchemistryduringthestudy: pH range(0-96hours): 7.9- 8.0(contreoxlposure) 7.9- 8.0 (1000 mg/L exposure) Temperature range (0-96hours): 20-21 OC Dissolved oxygen range (0-96hours): 7.8- 9.6mg/L (controelxposure) RESULTS 3.6- 9.6 mg/L (1000 mg/L exposure) Nominal concentrations: Bk controland 1000 mg/L Element values: 96-hourLC50 = >1000 rng/L Element values based on nominalconcentrations. Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206CE 96-hour LCw forfatheadminnow was determined tobe > 1000 mg/L. Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyJ:imischranking2. Testingmeets thecriterifaorqualittyesting. However, sample puritywas not properlycharacterizedand the studylacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Gl 358-2,1989. OTHER Last changed: 6/26/00 F-WIRON"GNTAL LABORATORY LR- FISH TOXICITY DATA SHEET DEFINITIVE- (RJLL-WAM TEST) TYPE TEST CAL TESTED LOT 67 TESR ORGANISM. @fite2u /tr-DATE REICI BATCH NO. AVG. AVG. SIZE 1'99 t9.ll@-rm NO- OF OGRANIS-IS PER IM7MW Za_ LOADING FACrOR--.O,xo g-@T. FISH DISEASE TMTMEW-&ACE TEST VESSEL: TDC Lirpr PYR glass_ Cylindri-cal tanic-TEST SOUJTION VOLUME a@ T. DATE STARTED Igg I ol-- DATE CCt4PLEM DILUTIOWNATERC-arh-fni1ntprpwde-1W-a1ter(Tem-p-.Zoor,D.0.q, mg,,/plH ANALY P,',U Lcso(95% ConfidenceLimitsl-- Ta,,iIknitial Total 24 H ou rs 48 Hou s 72 Hours 96 ou No. Conc. AmoLumn Total Nc. p[i 0. Total No pf['D.O.otal No. pH .0. otal No. pH D.0. mg/L Added Dead Pi/L Dead mg/L Dead Pi/L Dead mg/L T Control :T 9 191?Iz -la&A- -loan r/9 0 Test Temp. 0 c Initials&DaDOtCa@tee CCBMENTS: bipSECON14RY EFFECTS U;4 (UMM @rec-sv maw a &QpMrAi4f'@ 1000 MO MIAN MOITALITY) -i.cl P ],-Av- 4e leyi NO. Sywtoms or Reactions 24-Hr. -i*otaNi=er-Aft-eZfea 48-Hr. 72-Hr. 96-Hr. Test methodology conforms with the following two test protocols: (1) OECD 0203 "Fish, Acute Toxicity Test". (2) US-EPA IBG-9 "Fish Acute Toxicity Test".