Document zbx9Vxjv20NJyDBxrmRY6m8va
3M
3M Environmental Technology and Services
PO Box 33331 St. Paul, MN 55133-3331 612 778 6442
$-6H$ - 8C0~ 3 73
-0051
Laboratory Report
Report of Data for
Exploratory 28-Day Oral Toxicity Study in Rats:
Telomer Alcohol, Telomer Acrylate,
, PFHS, PFOS
Laboratory Report No. FACT-TOX-120.3
Requester Project No. NOTOX #242933
Testing Laboratory
3M Environmental Technology & Safety Services 3M Environmental Laboratory
Fluorine Analytical Chemistry Team (FACT) 2-3E-09
935 Bush Avenue, St Paul, MN 55106
CmCll i *---j
---DvjnmOT
-'-J C'23Crn3
ID
r r>
rc c u > - <- -
u
f
-i*;*'-
U-^ . C - L.
Cv
CS> rD
v^c
Laboratory Contact
Kris Hansen, Ph.D. Bldg. 2-3E-09 P.O.Box 3331
St. Paul, MN 55133-3331 Phone: (651)778-6018 FAX: (651) 778-6176
Requester Marv Case and Paul Lieder
3M Company Toxicology Services
220-2E-02 St. Paul, MN
Report issued 6/11/99
& E P A -O T S 000811788X
O G oaii7flax
000448
3M ENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-120
This study was designed to preliminarily assess the toxicity o fvarious test articles to die female Sprague Dawley ra t Test animals were dosed by oral gavage at the following levels:
Group 2: Telomer Alcohol Group 3: Telomer Acrylate Group 4: Group 5: PFHS G ro u p 6 :F F O S
30 mg/kg/day 30 mg/kg/day 30 mg/kg/day 10 mg/kg/day 3 mg/kg/day
W ithin each group, 8 animals were given test material each day for 28 days, and then sacrificed. Three animals were allowed to "reco v er (no test material) for 14 days before sacrifice; three animals were allowed to recover for 28 days before sacrifice.
liv e r samples collected from the sacrificed animals were sent to the Environmental Lab and analyzed quantitatively for perflnnmoctane sulfonate (PFQS) and other perfluorinated, sulfonate-based or carboxylic-- a d d based metabolites. (A full list o f acronyms and formulas is attached in Attachment B).
Tissue samples were homogenized (liver) and extracted using an ion-pairing extraction procedure. The .extracts were quantitatively analyzed using high-pressure liquid chromatograpby/electrospray tandem mass spectrometry (HLPC/ESMSMS), and evaluated versus an extracted curve. Analytical details are available in the study hinder maintained by FACT. The presence or absence ofknown fluorochemical contaminants and metabolites is determined by inspection
Six perfluorinated carboxylic adds were identified in the liver sample extracts derived from both group 2 and group 3 animals (see Attachment D).
Forgroup2 and group 3, livers from male and female animals in both groups ofrecovery animals showed a decrease in the concentration o f POAA. The concentration of two other carboxylic ad d based flnnnyhem iralg measured qnantitatively (M513, M613) did not show as significant a decrease over the
recovery period The concentrations ofthree other carboxylic add-based compounds could not be determined, as standard material is not available. Other perfluorinated or partiaHy-fluorinated metabolites may be present in the rat liver samples analyzed, but these compounds cannot be confirmed without standard material
Relative to PFOS levels in the corresponding group 6 animals,
(group 4) and PFHS (group 5) levels
determined in liver samples showed a dramatic decrease in the day 14 and day 28 recovery groups. W ithin
each group, samples showed a relatively large standard deviation which may indicate variability in dosing or
in metabolism o f the test compound
W ith the exception o fPFOS-dosed animals (group 6), there were dramatic differences in target analyte levels in livers o f male and female animals. For example, livers from 14 day recovery group 5 females animals were determined to contain two and a half enters o f magnitude less PFHS than the corresponding male animals
Although rigorous quality control measures and 3MEnvironmental Laboratory Standard Operating Procedures were followed, the acquisition o fthis data was not necessarily collected according to applicable Good Laboratory Practices. This data is not suitable for distribution to any government agency in defense of product suitability or safety. Data presented here is tire highest quality data available at this time.
This summary should notbe considered to be a complete inventory of all metabolites associated with the test m aterial Analyte measurements were made only on those compounds deemed by FACT as likely to be . present Certain other compounds have been identified but cannot be quantitated as standard material is not available.
000449
3M ENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-120
Liver samples arrived frozen at the Environmental Lab on 2/3/99. A chain-of-custody was not submitted w ith the samples, but was initiated upon receiptby die Environmental Lab.
There is no holding time criteria associated with these samples for LC/ESMSMS analysis.
Analytical methods FACT-M-1.0, Extraction o f Potassium PerQuooctanesulfonate or Other Anionic Photochem ical Surfactants from Liver for Analysis Using HPLC/ESMS FACTVM-2.0, Analysis ofFluorochemicals in Liver Extract Using HFLC-Electrospray/Mass Spectrometry
Sam ple p reparation - aqueous sam ples, HPLC/ESM SM S: ion-pairing extraction *. Analyte is extracted from a sample matrix with ion pairing reagent [tetrabulyl ammonium hydrogen sulfate
(TBA)] in a pH-controlled environmenL The cationic reagent selectively targets anionic fluorochemicals. Once die anion-TBApair is formed, die analyte is transferred into a non-polar organic solvent (methyl-terfbutyl ether),' dried, and reconstituted in methanol for MS analysis.
HPLC/ESM S and HPLC/ESM SM S: fo r detailed qualitative w ork . In HPLC, an aliquot of extract is injected and passed through a reverse phase liquid chromatographic
column. Based on the affinity o f the analyte for the stationary phase in the column relative to the liquid mobile phase, the analyte is retained for a characteristic amount of time. For example, in a standard solution PFOS may elute at 5.0 minutes. Retention times between a standard EPOS solution and the analyte extracted from liver in this analysis were matched on the HPLC system to within 1%. ... .
.
Following HPLC separation, ESMSMS provides a rapid and accurate means for analyzing a wide range o f
organic compounds, including fluorochemicals. Electrospray, an ionization technique used primarily for
detection o fmolecular ions, is generally operated at relatively m ild temperatures. Molecules are ionized,
and a primary inn, characteristic o f the analyte, is selected This ion is bombarded w ith high-energy gas;
subsequent collisions create smaller secondary ionic fragments unique to the primary ion, which are
detected.
,-x
For example, for PFOS (CgFnSQO analysis, ion 499 is selected as the characteristic primary ion. This ion is fragmented into other ions such as 80 am u (corresponding to SOs"), an d 230 amu (CjFsSCV). Each o f these secondary fragments is detected and can be used to differentiate PFOS from other compounds that m ight have the same characteristic 499 am u primary ion, but different chemical compositions and secondary ion fragmentation patterns.
5.1 Calibration
A mid-level, extracted matrix calibration check standard is analyzed every 5-10 samples to monitor instrumental d rift Calibration check standards are compliant if instrumental response is within +/- 30% o f the expected value.
0004S0
3MENVIRONMENTAL LABORATORY REPORTNO. FACT-TQX-120
Quantitation ofthe target analytes is based on linear regression analysis (weighted 1/x, if appropriate) o f two extracted matrix curves bracketing each group o f samples. Quantitation of each analyte is based in the response o f one or more specific daughter ions using the multiple response-monitoring mode o f the instrum ent
5.2 Blanks
Two extraction blanks, utilizing water as surrogate matrix, are extracted with each batch of samples. Additionally, two samples ofblank matrix, used for the extracted calibration curves, are extracted along with each batch of samples and curves.
Blanks are compliant if no target analyte is detected above the limit of detection for a specific analyte. In this study, all blanks were compliant
5.3 Surrogates
Tetra-bydro perfluorooctane sulfonate is used as a surrogate in this analysis. Surrogate response is monitored to confirm gross instrumental failure, if necessary.
5.4 M atrix Spikes
Duplicate matrix spike analyses utilizing all target analytes were prepared and analyzed for one control animal Except as noted in the results table, recoveries were within the acceptable range o f 70-130%. Recoveries outside this range may be re-evaluated.
5.5 Laboratory Control Samples
Laboratory Control Samples are not a component o f this study.
Six perfiuorinated carboxylic adds were identified in the liver sample extracts derived from both Group 2 and Group3 animals (see Attachment D).
For group2 and group 3, livers from male and female animals in both groups ofrecovery animals showed a decrease in the concentration of POAA Ih e concentration of two other carboxylic a d d based fluorochemicals measured quantitatively (M 513, MS13) did not show as significant a decrease over the recovery period. The concentrations of three other carboxylic arid-based compounds could not be determined, as standard material is not available. Other perfiuorinated or partiaUy-fluorinated metabolites may be present in the rat liver samples analyzed, but these compounds cannotbe confirmed without standard material
Relative to PFO Slevds in the corresponding group 6 animals,
(group 4) and PFHS (group 5) levels
determined in liver samples showed a dramatic decrease in the day 14 and day 28 recovery groups. W ithin
each group, samples showed a relativdy large standard deviation which may indicate variability in dosing or
in metabolism o fthe test compound
W ith the exception ofPFOS-dosed animals (group 6), there were dramatic differences in target analyte levels in livers o f male and female animals. For example, livers from 14 day recovery groups females animals were determined to contain two and a halforders o f magnitude less PFHS than the corresponding male animals
These results indicate that ingestion of each of the test materials leads to some accumulation o f metabolites in the liver. Relative rate ofdearance can be determined.
000451
3MENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-120
Samples and extracts willbe retained for future evaluation, if necessary. 9.1 A ttachm ent A : Table of Results o f Analysis o f Liver Samples 9.2 A ttachm ent B : Table of FACT acronyms and form ulas 9.3 A ttachm ent C : Graphical Results by Group and Gender 9.4 A ttachm ent D: Chromatographic results ofPFC A investigation in Group2 and Group 3 9.5 A ttachm ent E : Chain o f Custody 9.6 A ttachm ent F : Analytical D ata
i
000452
3 M Environm ental Laboratory Fluorine A nalytical Chem istry Team fFACQ
FACT-TOX-120, R at Livers 28-D ay O ral Tox Study b y D aily Gavage in Rats
PFOS (C8F 17S 0 3')
(Female) ..G1 '
G6
Dayl
Day 14
Day28
0.086+/-0.046 0.102+/-0.020 0.329+/-0.060
377+/-31
240+/-25
191+/-15
j[M ale2__
Dayl
Day 14
Day28
G1 0.133+/-0.030 0.255 +/- 0.122 0.099+/-0.007
G6
419+/-86
309+/-34
237+/-25
PFHS (C6F l3S O i )
(Female)
G1 G5
Dayl <MDL 97.0+/-14.7
Day 14
<MDL 0.26+/-0.12
Day28 <MDL 0.03+/-0.01
(Male) G1 G5
Dayl
Day 14
Day28
0.055 +/- 0.020 0.129+/-0.088 0.089+/-0.019
327+/-52 61.9+/-11.7 36.3+/-7.4
(Female) G1 G4
(Male) G1 G4
(7
Day 1 <MDL 0.37+/-0.38
Day 14 < MDL 0.02+/-0.00
Day28 <MDL <MDL
Dayl <MDL 3.09+/-1.58
Day 14
Day28
<MDL
<MDL
0.126+/-0.084 0.025 +/- 0.015
3M Confidential
000453
3 M Environm ental Laboratory Fluorine A nalytical Chem istry Team FACT
FACT-TOX-120, R at Livers 28-D ay O rai Tox Study b y D aily Gavage in Rats
POAA (C 7f 15c o 3 )
(Female) Gl G2 G3
(Male) Gl G2 G3
Dayl <MDL 0.164+/. 0.035 0.112+/-0.039
Day 14
<MDL 0.016+/-0.010
<MDL
Day28
<MDL <MDL < MDL
Day 1 <MDL 6.59+/-2.16 8.64+/-1.48
Day 14 <MDL 3.59+/-0.67 3.36+/-1.65
Day28
<MDL 1.76+/-0.23 1.26+/-0.44
MS13 (C9F 19C 0 2')
(Female) Gl G2 G3
___ (Male) Gl G2 G3
Dayl <MDL 1.69+/-0.18 1.20 +/-0.30
Dayl <MDL 1.91 +/- 0.88 1.78+/-0.31
Day 14 <MDL 1 .3 6 + /-0 .0 2 1.20+/-0.09
Day28 < MDL . 2.24+/-0.22 0 .8 1 2 + /-0 .1 8 8
Day 14 <MDL 1 .7 3 + /-0 .4 6 1.08 +/- 0.26
Day28 <MDL 1 .5 2 + /-0 .3 2 0.801 +/- 0.148
M613 (Cn F 23C O i)
(Female) Gl G2 G3 .
(Male) Gl G2 G3
Dayl <MDL 0.117+/-0.020 0.029+/-0.017
Day 14
<MDL 0.085+/-0.019 0.035 +/- Qj015
Day28
<MDL ' 0.101+/-0.012 0.036+/- 0.011
Dayl
<MDL 0.087+/-0.015
0.0622
Day 14 <MDL 0.107+/-0.041 <MDL
Day28
<MDL 0.106+/-0.017
< MDL
** C9, C l l and C13 perfluorinated carboxylic acids also detected in G2 and G3 animals 3M Confidential
000454
000455
FAC. :-no
2S-Day Oral Tox Stndy by Daily Gavaje la Rata
MfroditokclM nA o(rca S aM a); MutoMnU!
6 Softwm/VcnioK DM TEraaoka/AatlyM: DM *fAaalyili/Aailya: DM TDM R eM M A adj;
Sim ple Data
TO X -!2C lExplorM (y2U )*yO nlTaxkityStsdybyD n!yG m geiiX
T-7023,T-7Q24, T-7022, T-7032, T-4295
iM L iw
IH om bc:
FACTMI4St PACMK-2.0
R-Squred V ahe
So^COOl, H u tte 041090A--fil 0C49I
Siepe
M d.yn3.l
Y -h tan p t
2/22*9 RWW/SAH
02/23* 9, 03/01* 9, 03/ 11* 9, 05/23*9 ho j/m b b /d r b
02/2S*X 03/00*9, <0/12/99,05/26*9 ME8/DKB/KJH/H0J
to A m tlw e iM faeA U M teau Sm AttacfctMett
RATUVER Gmp Dm
M eted B& M eted BUt M aris BUt M arijB ft QC-M O n*
QC-M OfH
Greep 1 ana(W qr
OM I
G m pl 0.0 a g i o te Rm m o vM 4 D m
C risp OOafteM qr iM v a v l'ltD m
CffM f> 30.0pAffidap Tdom a Akohel
C rap l 3 0 .0 a fi|9 t)r Recovery 1 !4Dyv
C rap l 30.0fA f/day Recovar 2-2$ Dey
C raf] 30.0apg/dry Tckw rA nyiM
GreopS
Recovay I 14Deyt rap i
N O opldqr Recovar 2 21 Poye
Sim ple
EstrsdedW k 1
H20Bik-l H20B&-2 X iU llU n rB ir R teciU verB lk-2
106F 106F 1IQF IIOF
97F 91F 99F 100F
101F 102F 103F 104F 105F 106F 107F 109F 110F
II1F
114F
115F II6F 117F IIBF 119F 120F 12IF 122F 123F 129F 126F 127F I29F . 130F 131F 132F 133F 134F 135F 136F I37F 130F 139F 141F 142F 143F
NA NA NA NA 0.9240 0.9240
0.9242 0.9242 0 2 I 0.4339 03106 0390* 0.6970 0.4313 0.4901 OII16 0.6936 0.9240 0.43S9 0.4110 0920
0.9429
03506 0.649S 16769 0.7235 0.6534 0.4162 03791 0.4295 0.0372 0.6291 03660 0.1074 09112 0.6006 0.4M1
0.3012 0.7303 0.5990 0.3011 0.6131 0.5369 03799 RIOM 0.6235 0.5600
0.6693 0.7953 0.7303
MS C ifeC ac.
Fl <MDL <MDL <MDL <MDL 251 250
99
190 67.2 70.1 64.9 9007 Ni 653 62.0 119 106 00.6 344 379 263
4L3 33.7
37.1 364 OU 564 30.0
294 354
314 473 4U 513 524 343 3M . 514
413 494 453 35.7 46.1 17.9 194 43.9 42.4 413 403
T etti A m eut eTPVOS
ufi <MDL <MDL <MDL . <MDL MK S4K 23% 3394 0190 00672 00701 00649 941 . 00765 00655 00630 0119 0106 00006 0344 0379 0263
00412 00937 O03TI 00366 00016 00569 00310 00295 00356 00354 00473
00452 00505 00520
o.aW i 00304 00516 00415 . 00496 / 00152 00357 00460
00179 04194 00439 00424 00413 00402
M ctM Dcicctm Liait (M Dt F fO S 23*fe,raA A -S *fcM 5U -2S m dS O ^rg,M S13*25*fe
A vertit FFOS Fl <MDL <MDL 15% 2394
00063 130 0103 0329
00443 00394 00519
00429 00271 00413
Dm Eatocd/Anly*: DM VsifUd/Aaaiyvt:
02/2699,63/01/99,03/22*9,05/2699 LAC 06*0*9,06/09*9 EAD
RSD M .D cv.
<MDL <MDL
394 NK
53.4 >00461*
263 3.15 193 00196 113 00597
314 00171
173 000614
124 000667
154 000644
519 00146 170 000112
FOAA Cale. Cese,
rafie <MDL <MDL <MDL <MDL
219 267 i k 17172 0.00 OOO OOO 000 OOO OOO OOO OOO OOO OOO 0.00 OOO OOO OOO
13 ns
163 160 105 196 223 139 243
433 17.4 0 0 0
4J 105 954 166 124 906 170 75.1 OOO 000 OOO OOO OOO OOO
T etti A m ari efFOAA
rt <MDL <MPL <MDL <MDL 7394 0994 75% 53%
<MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL
<MDL <MDL <MDL
0135 0115 0163 0160 0105 0196 0223 0139 04245 000453 00174 <MDL
<MDL <MDL
00665 0105 00950 0166 0124 00906 0170 00751 <MDL <MDL <MDL
<MDL <MDL <MDL
NX NoiR^orted
N/A-Not Applicale
m s-
K )A A M513-C9P19COO-
16613* C lIF23CDO
A verti FOAA Fk <MDL <MDL 01% 66%
<MDL <MDL <MDL
0164 0015) <MDL
0112 <MDL <MDL
RSD StODcv. <MDL MDL
20% 25%
<MDL <MDL <MDL
ILS 00354 6X4 00101 MDL
354 04390 MDL MDL
Citc.Ceec>-
Ft MDL MDL MDL <MDL
122 193 122 193
OOO OOO VALUS OOO OOO OOO . VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS
VaLUb VALUS VALUB
VALUS VALUS VALUS VALUS VALUS VALUS VALUB
VALUS VALUS VALUS VALUS avau VALUB VALUB VALUB VALUB VALUS VALUB VALUS VALUS VALUS VALUS VALUS VALUS VALUS
T etti A eusat ef
Fl <MDL
<MDL <MDL <M0L 41% 65% 41% 65%
<10 H* to n *
NR
<n* n * to n *
NR NR NR NR NR NR NR NR
NR NR NR NR NR
NR NR NR NR NR NR NR NR . NR NR
NR NR
NR NR NI NR NR NR Ml NR NR NR NR
A vertit Fl <MDL <MDL 53% 53%
NR NR NR
NR NR NR
NR NR NR
A d. Dm <MDL <MDL 46% 46%
NR NR NR
NR NR NR
NR NR NR
FACT-M-2.0 Excd Vtniom 7.0
TX*120-Imr.ila
6T10*9 5:12 PU
PS
fi
3, M
** H
l||
Hi
li 1
!i<
Si?S
i|I !i?i?- p
S
!)!
! r
|i
!! r
88 j? l2 %1991f99f ri
Il
^
ili ili iiiiiiii
iii iii iiiiiiii
i
i
i
i
l
i
11111*33
il
il
f
il I!
i
i l *1
II
Lsa
1 P
i!*K3p p fpii l i
il P I
iiiiiiii
p pp
ISS i l i
ii
iiii
lit
fllifi 111
lilil
8tP tPSilp3333 ^ir_--
v`f-Vi-
-f$
PiiSSIjjji i l Bggig;
I I ! S M ! 'iilSf.U Mi *
5333333333335355333333355555 33335353353535ZZ SzSi II II * i|
r
!
*r 3 3 3 ?3
555 535
335 553
Z I 1 1 U|
ii 1 1 f S
si
HI
Iiit
| |I I sai sis sisaisil
ili
sSSSSSSl liP P P 888
pppppppp 88888888
53S3P?MiTl*
Sl 'fp.
! ~ sp sp*- =
f
'siti
gg
||
Efpsss
Ml IMi i i i i i i i
II n
II M.**1}
: i Ii s: i s
gS Si15
S
11i
3 3 1 1 *I
K g|3* U
K se s
11s
i Z 1 1 <
HcS "Si IlisSSIiassn m u 523
SSSSciSS
P 8
P 8
P 8
88
8
S 3 2 3 II II i
ili fil gmP P P p p p iiiiiiii
P
a
P
Ili
gpSpSpSp cpip5p5Mi
Ml IIIIIIII
*
IIMl
*
II
II
r &* * S
i
L
i
ii i
5gp u
2 5 3- SC
i i1 i 1 1 sH1s i 1. r
1 3 1 1 3m S J|
I3 z 1 1
Si=1 000456
000457
---------- FA.------- .M20 U 2S-Day Oral Tox Study by Daily Gavage la Rala
Slady;
h o * * >l--t a f f a i SaM fcc M ttic
McfboMcvbn:
A aatytiealEqaipN tSyriaNaari battane* Somc/Vmbo: DricofExttactioarAaalyri: DatoAaayiu/Aatfym: Datea f Data RafeetWAaalyri:
S a a p ltD iU
TOX>120,Erphmtoiy 23-DayOralToxicityStadyby Da&yQ m |t fa Rata
T-7023.T-7Q24, T-7022, T-7032, T*4293
RMLiver
FACT-M-U A FAC44-2.0
R-SqaandVabe
' Maddka 041093, Amelia 062498, d Soap030199
AteLynl.1
Sope Y-aiattpL
3/22/99 RWW/SAH
0Z4/99, OSa1/99,03/14/99,0*2*9 HQjPMEEftttB
03*999,03/12/99.03/17/99.03/2499 DRB/KJH/HOJ
S et A iuchacott
RAT LIVER Graap Dw
Method Btk Method BOt M rtrM k Mris Blk JC-250 R*
<X^250r *
C rm /4
Cr*4 30.0 Boif/day Racttvay I 14D m
C reap4 30.0 e ifttty Recovery 2>2t D m
C rup! lO.Omttyigy
ma
GroopS io .o i|k |io r Recover 1- 14D m
Cm pS
Recovery2-23 D m Creep 4
3.0m fftffrqr nos
G m pt 3.0n*k^ey Recovery I -14 D m
Creep 4 i.0m$/kt/f Recovery 2-23 D m
-ph r
Extractad Wi. S
H20Blk~l
K 20H M Rabbit Liver B ft-l Rabbit Liver Bfk-2
104F 106F
llflF 110F
I4SF 144F 147F I4IF 149F
190F 151F 1S2F 153F 194F 155F
157F I5SF 139F
141F 142F
143F 144F 145F 146F
147F (41F
149F 170F 171F 17P 174F 175F
177F 171F
179F IMF IflF II2F 113F IMF II5F IMF IC7P IS9F 190F 191F
NA HA HA
NA 0.9240
0.9240
&9 0.9242
0.3449 0.4113 0.4437 0.4699 04097
0.4416 03474
0.4341 0.1571 07221
0.1019
0 J1 II 03430 0.9194
0.9399 0,404
013107 0.4779
0.7719 0.4252
0.4732 0.4373
04394 0.7737
0l547I 0.1744 0.7937 0.4424
0.7352 0.4423
0l5442 0.3104 U7I04 04940 0.3512 0.3207 1.0370
0.3345 0.7563 01719 04832 09180
rr i
C rib C w .
afr <MDC <MDL MDL <MDL 253
250 49 99
144 118.7 02 130
102.7 103.0
150 11U 135 71.7 142 94.8
142
99.1 44.7
77.1 117.6
702 72.7
1207 903 45J 434 41.8 564 4M 115-9
34S9I1 333073 334730 333395 422128 404233 343202 325332
213147 247344 235351 132143 183230 201253
T itd A aw d
arm s
fr <MDL <MDL <MDL
<MDL SM MH
23% 33%
0.U4 01137 00902 0130 01027
01030 0150 01114 00774 0135 00717 0142 00948 0142
00991 00447
00772 01174
00742 O07Z7
0121 00903 00459 00435 00418 00545 00435 0114
364 333 337 333 422
/ * 343 325 218 243 235 112 133 203
A m asa FFOS afe <MDL <MDL 15% 28%
OtlSS 00948 0140
00394 00571 00734
377 240 191
RS StODtVe
<MDL <MDL 00304 0354
17. 0.0211 364 00350 24.3 00374
23.4 00212 234 00133 500 OQ36I
49 314 105 234 7.72 14.1
MethodDdectioa Limit(MDL); F F 0 3 -2 4 * 1 2 4 a 9 fr,r0 A A -1 2 4 artl - S P F H S - 3 ftM S D -50a tffrM * -S a ft
FOAA Cafc-Caac
hfr <MDL <MDC <MDL <MDL
219
247 223 173 0.00
ooo OQO ooo ooo
ooo ooo 0.00
ooo ooo ooo
0.00 ooo ooo
ooo ooo ooo ooo 000 ooo
ooo ooo ooo ooo ooo
ooo ooo
ooo O.oo
ooo 0.00 ooo 0.00 ooo Ooo ooo 0.00 OOO ooo ooo ooo ooo
TatalA aaaat rroA A
aefr
<MDL <MOL <MDL
<MDL 73% 39% 75% 53%
<MDL <3401. <MDL <MDL
<MDL <MDL <MDL
<MDL <MDL <MDL
<MDL <MDL <MDL <MDL <v4bL <MDL
MDC <MDL <MDL
<MDL <MDL <MDL
<MDL <MDL <MDL <MDL <MDL
<MDL
<MDL <MDL
<MDL <MDL <MDL <MDL <MDL
<MDL
<UDL <MDL <MDL <MDL
<MDL <MDL
Avana FOAA rt <MDL <MDL 81% 44%
<MDL <MDL <MDL
MDL <MDL <MDL
<MDL <MDL <MDL
N/A*Not Applicable
FFOS-tatemoctaw
FOAA-M aorooctiai
Dria&actad/Aaalyri: DrieVcrifietfAttiljei:
ta n tm .m n a m .ta n im .ta m .o m m lag 0698/99,06/0499 EA
FFHS-F M S13-C9FI9000M413-CUF23COO-
RSD StfoDtv.
44DL
C alc.C aac
aofr ,<UDL .
<MDL 0199
f i '.tU D L
-U ,, IM .. >;>*-193 *'
0254
M l* !^ ?v*-.-;i40,
# = w a f
m ot.
<MDC ,
<MDL
? !V io i & * .* * .. .
V & $ > * . :f M O '
i3 ao io o > ' i'M O -'-v . A .jM O
ili # <MDL ^ ^ 0 7 v if-
<UDL
<MDL
W lh m m ^^sOOO-j'-f^ ^ 0 0 ,0 1 ^ -7
? ^ ftO O ;' L & t M : - ' 1-
<MDL <MDL
fe # !a o o .' 2&&040 </';
as|.o--<c
<MDt ^ ^ roolvv
a^t <MDL
<MDL <MDL <MDL
41% 45%
41% 45% 0140 00309 0.1M 0428 1.19 <MDL 0491 040!
0.0143 0.0146 010115 0.00869
NR NR
Wl NR
NR NR NR
NR NR NR NR NR NR NR NR NR
U NR NR NR NR NR NR NR NR
NR NR NR NR NR
A <MDL <MDL 53% 53%
RSD *M,Dv.
<MDL <MDL 0454 0i454
0375 00145 0.00349
101 0477
114 000194
DWM 4DIV/M
NR NR NR NR NR NR
NR NR NR NR NR NR
FACT-M-2.0 E w d V erioa 7.0
TOX.1204MMJt
1099
*12 FU
1 i
000458
2t-Dy O rai Tox Stndy by Daily Gavage la Rais
Stady: Froda* N n to fr a l S ab a to *): M atrix: M adtod/Rcririoa: A n ljitie tlE p p w a ly tta N n k R ta n n a m i S aftw aa/V ariaa: Data o fExtrartiner/Aaalyc D ocefA aalyririA adyae Data a fData K aterioaM ariyac
S a a ip fo D a ta
TOX'!20lEapfentoty 2-0*yOraiToxidfySMy by DaDyG am e ia R iti
T-7023, T-7024, T-7022. T-7012. T-6295
RM livar
* rm iir
Settinto riffe
FACT-M-l.O* FACT-M-2.0
R-SqaaraJValeeSee A ttedano*
Madrina 041090,Aatriia 061491, aadSaty 020199 Stop: *
SccA itacfeM i
M aaLynx3.t
Y*fartoccpe SceAaachtfnti
2/22/99 RWW/SAH
02/2V99,03/1t/99,03/1699,05/2599 HOJ/MEE/DRB
03AV99,03/12/9, 03/17/99,05/2699 DRB/XJH/HQJ
R A T L tV E R
Cmp
S aap ltf
KxtndcdW t.
I rti
Daaa E O le. CMC.
MetfaodBlk
KMBlk-l
1*1 NA *<D L
MctfaodBlk
H20Bk-2
NA "*MDL
Matrix BUt
R iM iU w m n'
NA
<M)L
M ani Bit Q C-Jw
RAtfcU va BIk-2 I46F I06P
NA 0.9240
09240
. :-MDL' 'xl**. r a s iv i
QC-2M H*
1I0P 09242
1I0F 09242
Crea* 4
14F 03643
DUO * * * ,
I46F
04113
< M ***if'
I47P 04457
I40F 04699
I49P
0.6097
ao/
ISO?
06416
'tM
C rae*4
I51F 09476 ;V & 000 &
132F 0.6341 'v v ;O 0 0 -':>
lilF
0.1576
..oo
3 0 .0 n tty d a y
t$4F 07221
Iceavov 1-14 D m
1S9F
0.MI9
M m W :
C raaf 4
137F O liti
3 0 9 a tfttta r Recovery2-21 D m
I9SF I39F
03430 09196
G rafi tO .O af'if/dej'
K i r ..... 142F
o .n 04004
E5m 100196
ma
I63F
01107
94742
I64F
04779
91925
16SF
07719
116992
166F
04232
19141
I67F
04732
114702
IMF
06371
76152
G rafi
tOLOas/kfVqr Recovery 1-14 D m
M9F 170F I7IF
03394 07757 05671
393 169 226
C raff lO daf/kfU cy
Xceaverv2 2* D m C rafl
.0 m jkj * y
173F I74F I7SF 1771*
I7SF
01766 07937 04626
o ith 04423
373 273 31.6
FFOS
I79F 05462
IMF 00106
IIIF 07104
1I2F
05940
: .'0oqv
I03F
01912
c OM
IMF
01207
-- OOO
G rafi 3.0mfkftay
Raeavarv 1 14D m G rafi
3.0 ls*ay RacararyZ-Sf Deya
1I5F IMF 1I7F II9F I90P 191F
1.0570 01343 07560 00709 06002 09100
ooo V,.?AOOO y;-:.
M #, S aiL B m -
LhafQaantitana(LOQ):
FOAA- 23 . t
MadaodOrtarhaiItorti(MDL): FFOS-2J*t2_Sa*'*,FOA-l2Ja
T attiA n-- t afFFHS
e/ | MDL
MDL MDL MDL
0% OS OK
OS NR
NO NR NR NR NR NR
NR NR NR NR NR NR NR
4.6 to t 94.7 91.9 117 09.1
115 702 0393 0169 0226
.0372 00272 00316
US NR NR NR
NR NR NR NR NR
NR NR NR NR NR
Dato Eafand/Aaalyat: Dato V oiBol/A aalyat:
02/26/99,03/10/99,0V 1 7 /9 9 ,03/22/9, OS/2 0006/99,06/09/99 BAD
A vente FFBS rt <MDL MDL OS OK
NR NR NR
97.1 0363 00320
NR NR NR
RSD M513
S ii. Dar.
Cale C ase.
<MDL
tft <MDL <MDL
MDL
MDL
MDL
#d v /o! fDIVAH
276
212 179 162
OOO OOO OOO
OOO
OOO
OOO
OOO
NR OOO OOO
OOO NR OOO
OOO
OOO NR OOO
OOO
OOO . OOO
0.00
OOO
OOO
153 0.00 14.7 OOO
443 0116
OOO OOO OOO OOO
13.7 000502
OOO OOO
0 OOO
OOO 0.00
OOO
0.00 OM NR OM
OM
OM NR 0.00
OM
OM
NR NRNetRcpofted
OOO
N /A -N atA ppfeaM a
FTOS-
F O A A * PcrflaonocUBO itt
PFHSM513 C9F19COO-
M6t3CtlF23COO*
T atti A neeat
afMS13
Fi <MDL
MDL .' MDL
MDL
92S 94% 60%
54%
MDL MDL MDL
MDL MDL OflX.
MDL MDL mU . MDL MDL
MDL MDL MDL
MDL MDL <MDL
MDL MDL MDL
MDL MDL MDL MDL MDL MDL MDL MDL
MDL MDL MDL ^ MDL MDL MDL MDL ' MDL MDL MDL
MDL MDL MDL MDL
A rante MS13 afe _ MDL MDL 93% 57%
MDL MDL MDL
MDL 4ID L MDL
MDL MDL MDL
BSD $14.Dar. MDL MDL 00205
0103.
40L MDL MDL
MDL m d l MDL
MDL MDL MDL
M613
CafeCaoc.
tfe MDL
MDL MDL MDL 267 314 272
241
OOO OOO OOO OOO OOO
OOO OOO OOO OOO OOO OOO OOO OOO OOO
OOO OOO OOO OM OOO OM OM OM OM OOO OM OM OM OM &eo OOO OOO OOO OOO OM OM O.M
OM OM OOO OOO OOO OM
T etti Am avi
afM613
ttfe MDL
MDL MDL MDL 19% 105%
91% 11%
MDL MDL MDL MDL MDL . MDL MDL <M>L MDL MDL MDL MDL MDL MDL
MDL MDL MDL ` MDL MDL MDL MDL
MDL MDL MDL MDL MDL MDL MDL
MDL MDL MDL MDL MDL MDL MDL <M>L MDL
MDL MDL MDL MDL MDL
A vente M613 *fe MDL MDL 97% MS
MDL MDL MDL
MDL MDL MDL
MDL MDL ' MDL
RSD 814. Dav. MDL MDL
0161 0123
MDL 44DL MDL
MDL <4DL MDL
MDL MDL MDL
FACT-M-2.0 Excd V aria 7.0
T O X -l204tar.iti
6/10/99 3:12 FM
000459
--------- i FA <*--+-otC-1 2 0 l-- a
2S-Day Oral Toz Study by Dally Gava{e la Rats
hadiK (M ada(T (ttd(laaN )! M ak M abodUcviM :
b aran e* SoftwareWcmoa; D aeofBUftctlw/Afra? DneaTAaalyatfAMlya: Datea f Dal RedBCtmfAaily:
S ta p le D ata
TOX-12R E q teao iy 2M )eyOnl ToxfcfcySttdy ly Da3yGsvagefa Rue
T-7023, T.7Q24 T-7022, T.7Q32,T-6235
KMLiver
m enane:
FACT-M-l.Q* FACT-M-10
R-Sqeacd V ette
M ediae 04109,Soap 020199
Rope:
M wLynU
Y ^M cqc
Q4M/99 RWW0CMAK
04/2099, Q5/14A9.0S/2V99 HQI/DRB?
P4/22/9R0V0S/99,05/11/99,05/26/99 KJH/MEE/DRB/HOJ
RA T LIV ER C rap Deec
H n l |tt 0
MnrixBtk 0
Q C .B O frk
QC-M On*
Q C -2b*
C nepl
Cosmi
C rem i O.Onpli|/4oy Recovery I *14 Deve
C respi # 0 .,* a ttq r Recovery 2-21 Deye
C m pt 3 0 .0npt|ay Tdorecr Akshel
C respi X .O s^ tfto y Recovery 1 14Dave
G rasp* 30.0 *(/*/*? Recovery2 -2 f Deye
C rw p) 3 0 .0 n g ftfttq r T rin irr Araytele
G roaf) 3Q.0nt/kgfeey Recovery 1- 14Deyi
G rafi M On^hfM m Recovery 2 2 t Deye
S anplel
H 20Kk-l H20 80^2 KjMit L ira Blk-1 RabW LiverKk-2 3M-MS 1M4ISD 6U-MS A U ISD 13M-MS U IM BD
iU
2M 3M 4M 5M 4M 711 SM m 10M 11M I3M I4M I5M
17M ISM 19M 20M 2IM 22M 23M 24M 25M 2SM 27M DM 30M 3IM DM 34M 35M 36M 37M 3SM 39M 40M 4IM 42M 43M 4SM 46M 47M
k rtn d id W l
I
0.00 0.00 0.00 0.00
0.91! 0.9162 0.9091 0J09S 1.0701 1.0701 0.6511 9.6999 0.9162 0.6124 0.1920
0.909S 0.1965 0.1267
R6344 0.6972 0.1504
1.0701 0.9044 0.SS1I
1.0503 0.9993 04139 0.5331 0l7957 1.0663 0.7241 0.5990 0.56IS 0:7356 0.9176 0.9513 . 1.0SS5 0.9514 0.6419
0.6715 04SM 0.9225 049JJ #.574 017240 016670 1.0153 0.714] 0.9065 1.0667 04633 0.9711
nw Ci Jc-Cmc.
Ik
<MDL <MDL <MDL
<MDL 274 2S6
319 300 2S9 291
Hi
129 102 124 104
13S 127 165 115 239 312 944
107 95.7
' 4 " 404 39.7 36.4 34.6 254
194 294
319 26.0
254 444 93J 91.7 70.4 504
654 5S4 764 4S.7
H.
915 41.4 64J 494 59.7 511 . 56.6
T etti Aneaat efFFOS
<MDL <MDL <m d I
<MDL 92% 96% 107% 103% 97% 97% Oliti 0.129 0.102 0.124 0.104 0.131 0427 0.165 RI 15 0439 04119 R094 RIOT 0.096
R0S4S R04Q2 RQ397 R0966 R0346 R0259 ' R0192 R0290
R0329 0.0260 R0255 R0449 R093S R03I7
0.0704 R0509
. R0652 /0.Q5S0
R0762 R04I7 ROSO# R0925 55414 R0645 R0499 04597
R053I R0566
_
Average FFDS afe <MDL <MDL 94% 105% 97%
RI33 0455 R099
0.0350 0.0211 R056I
ROSTS 0.0SI9 RQS65
MeMedD e tta i link(M DL): fF O S -24m fcrO A A -Sm fem iS-24m fe.<S13-2SaadS0m fcM 613-25m fe
DaeEnered/Aaaly*: Dale Vcrtfed/AaetyM:
05/05/9R05/2699 LAC M INM ,0fM IDEAD
RSD Sti# Dev.
<MDL <MDL
4% 4% 04
20 R03 474 0.1220 6.97 R0069
304 R0107
144 R604I6
574 R03272
224 ROI515
215 R0I17 5.W R0Q327
- M aa Ci R C mc.
afe <MDL <MDL DTV/0I 6MVAM . 254 260 29943 31R30 344.99 31171 R00 R00 0.00 040 0.00 R00 0.00 040 RQO 0.00
RQO RQO RQO 0.00
5077 3415 1366 7910 4944 7045 5713 10045 4197 3712 2*73 14*9 1662 2026
6005 10321 1091 1559 1463 10095 7591 10021 4711
1545 3764 ttS7 SS7 1731
T ettiA m u* errOAA
afe <MDL <MDL DIV/W DIV/M 15% 17% 100% 104% ' 115% 105% MDL <MDL <MDL <MDL
. <KL
<MDL <MDL <MDL
<mdC
<MDL <MDL <MDL <MDL MDL
it 3.49 147 741 444 7.04 5.71 10.0 449 171 1S7 IJ9 L66 103
6.01
IR3 1.09 146 R46 10.1 749 10.0 4.7S 145 176 1.16 R II7 1.74
N /A -NrtAff& aNe
.FTOSPOAA-PerfiaenoclanoMa FFHSF
Msts*cmooo-
M613 ClIF23COO-
Average FOAA afe <MDL DTV/M 16% 102% 110%
SDIV/N D1VAH DIV/M
159 349 1.76 .
164 136 146
RSD SU . Dev.
<MDL 6DIV/M
2% 3% RIO
6DIV/QI HXVAN
6DIVAI
317 116 111 0.669 113 0434
17.1
US
494 145 344 0.435
C SbC ab
ffe rat rat rat rat VALUE! fVALUEl
6VALUEI VALUE! VALUE! VALUEI (VALUEI VALUE! VAUIB VALUEI VALISI VALUE! VALUEI VALUE! VALUEI VALUE! VALUEI VaLi VALUE! VALUE!
VALUE! VALUE! VALUE! VALUEI VALUEI VALUEI VALUEI VALUEI (VALUEI VALUEI VALUEI VALUE! VALUEI VALUEI
(VALUEI
VALUEI VALUE! VALUE! VALUE! VALUEI VALUE! VALUEI VALUEI VALUE! VALUE! VALUE! VALUE! VALISI
T etti Aeeesat
r
a rt * rat
rat rat NR
rat rat
rat rat
rat rat
ita rat
rat rat rat
rat
H*
NR NR
rat
rat
ra t
ra t ra t
rat ra t ra t rat NR rat
ra t rat
rat NR NR
NR NR
NtaR
ra t
NR NR NR NR NR NR
NR NR
NR NR
NR NR
Average afe NR rat rat NR rat
NR
rat rat
rat NR NR
rat rat
rat
Ub
SU. Dnr. NR NR rat rat rat
rat
rat rat
NR
rat
NR
rat rat
NR
FACT-M-2.0 B u d V cntta 7.0
T O X -12aW jtte
ff!99 5:12 FM
000460
----------- F A C ,- ___ . -1 2 0 -------------
*.
2S-D*y Oral Tox Study by Dally Cavate In Rata
FrariaitN iak i(T q l Matrix: McAad/XctUsB AaatyrieM B oa fem tt S yitca N m to n
IllW X M tio ftW W fV d lltK D rtcafE xfractiaa'A aal}:
D ataafAaatyria/Aaaly: Data a fData E rria rtin a iA a riy f
S ia p b Data
TOX-1M , Exploratory Il-O a y O ral T a xfe ityS ttriyky D rily C avatala tota
T.702J, T-7024, T-7022. T-7032.T-4295
MmUm
F ilttm a r
S c c to tia rifb t
PACT-M-U * FACT-M-LO
I-S g u n t Vata c Sea Attor ta l a
M idrib 04109,S o * 02819
MmLynU
S lept: Y4MaNfC
S ttA s a d ttM i SetA ttaccatati
04AW9 RWW0CW5AH
04/20*9, 05/149,05/25/99 HOJ/DRB?
04/22/,o s jw n . e s /ii/t* w w n kjh/mee/drb/ho j
RAT LIVER Creep Daaa
fclwk^PIV 0
Matrix BOc 0
o c - io n *
QC-MOb *
QC-JKb *
C raapl M aj/kfU ur
Coeeal
C raapl OOaf/fcfttay Oecevov t 14D m
Craap t OAopfeptrisy Recovery2 *28 D m
Craap 2 3 0 0 ajftffey T c to sA k to l
C raapl M Aa^fciM ay lU tavav t 14D m
C raapl 39B*kpUqr Recomy 2 2! Dayt
C raapl M o a ito to TaleaiarAciytot
C raapl R O agiaU qf Recavtrv l*14D m
G raapl 390B f/k|toy Recovery2 21 D m
la a p itl
Estrattori WtT 1
H20BU-1 K30BJk>2 R attit LiverBik-I Ratok LiverBSk-2
3M-MSO 6MMS AMBD 12M-MS 13M-MSD
tii 2M 3M 4M 5M
<M 7M tM Ml I0M 1IM 13M MM ISM
I7M ISM SMI 20M SIM 22M DM . 24M 2SM 2CM 2711
29M MM 31M
llM StM ISM MM 37M 3SM MM 40M 4IM 42M 41M J
46M 47M
DM 600 900 0.00 09K 2 0.9102 0.9091 0909 1.0701 1.0701
OASIS 0.4999 0.9142
0l4S24
0.8920
09090 OS94S 0.1247 0.C344 014972 0.IS04 1.0701 0.9044 08511 .
l.ttSOl 09992 04*39 0.5331
07957 1.0642 01724 95990
Q.S41S 0.7354 0.917 99SI3 1.0055 0.9314 .48*
96715 OJSSO 99225 95915 0.5744 0.7240 0.4479 1.01S3 017143 0.9063 1.0647 03633 0.9711
rn u Calc. Caac>
A iCMDL <MDL <MDL <MDL
273 2S4 219 211 247 291
49.0 96.6 49.7 50.9
1 54.7 40J 41.9 443 221 122 101 90.4 4M 34.4
45 SM 112.1 214
1U 12.0
29J 24J
I4 J 22.8
244 44.1 21.1
444 S it 991
j / 417
$63 50.7
104 217 22.4 21.1 29.1 304 274
T atti A aeeat a rrm
fe <MDL <MDL <MDL <MDL 91% SM 107% 104% 19% 97% 0 .0 M .......... 0.0964 0.0497 0.0509 0.0312 0.0547 00400 04> 00442 0221 0122 OlOS 00904 00419.
00344 00455 90514
O lii 00254 0.0284
00220
00299 00249 00149
0022 5 5 3
00441 00211 oo44 00531 00901 0.0453 00447 00541 00507 91038 00227 00234 00311 00298 0090 0027
Avarapa DUI n ft <MDL <MDL 88% 105% 93%
0054 0129 00190
00440 00255 08332
00663 0.0291 00293
W ftO D av.
<MDL <MDL
7% 2% 90S
374 00204 0 00184 2I.C 00194
624 0021 402 00103 34.1 9 0 !!
309 00205
194 000559
142 0.00159
MJ1J Cric. Ciac,
c^l <MDL <MDL
<MDL <MDL
225 227 239J5 29340 217.83 247.4 900 900 900 0.00 0.00 900 OOO 0.00 OOO OOO OOO OOO OOO OOO
102 415 1485 2253 3155
2007 2021 2720 1491 224 1443 1844
1222 1475 2304 2001 1727 1775 1853 1234 1489 1428 788 1174 1270 492 969 741
Tatti Aawaal afM Sll
P i <MDL <MDL <MDL <MDL fe% 74% 80% 98% 73% 90%
<MDL <MDL <MDL <MDL <MDL <MDL
<MDL <MDL <MDL MDL <MDL <MDL <MDL <MDL
MB 041 L4t
245
11
101
102
172 i.4 t 24 144 1.8 142 1.47
240
100 1.72 L7T MS 144 1.69
1.42
4 .H 1.17 1.27 0492 9969 974t
A varila MSU alfe <MDL <MDL 76% 9% 81%
<MDL <MDL <MDL
Ut 1.73 . 142
1.78 LOI OIOI
*b' 9tri.DVe
<MDL <MDL
1% 20% 021
. <MDL
MDL
<MDL
460 087 266 0441 214 0321
174 0307 217 0455 184 0148
-- m *-- C ric Ciac.
*fc <MDL <MDL <MDL <MDL
241 22 m* 21140 22541 23171 i OOO OOO OOO . OOO OOO OOO OOO 000 OOO
OOO OOO OOO OOO
904 494 814 74.7 994 714 960
112 <14
11 142 113 164
11
S5
00 24 OOO OOO OOO OOO OOO OOO OOO 000 OOO OOO 800
M otoriDcttctioaLtoJl(MDL); W p S -li * /|,P O A A S ip fcm
Doa EatcnrifAaalyat: Data V crifiO P Aaalyit:
0505/99.05/24/99 LAC O M W A O M M fE A D
W A -N * Applicato
wos-
POAAITH 1 - frrflawTihnat M513 C9P19COO-
M 6U -C U R 3 C O O .
Votai AawaT" TM413
rt <M0L <MDL <MDL <MDL 1% 77% 43% 71% 76% 71%
<MDL <MDL <MDL <MDL MDL
<UDL <L <MDL <MDL MDL MDL <MDL MDL 4ID L
00904 00493 90834 00747 00995
00724 00940
0112 00416 0119 0142 0113 9006 O li
MDL MDL 00422 <MDL <MDL <MDL <MDL <MDL MDL
<MDL <MDL <MDL <MDL MDL
A varila M413 MDL MDL 79% <7% 77%
<MDL <MDL MDL
00172 0107 010
00622 MDL MDL
USD StOPcv. <MDL <MDL
5% 12% 90S
<MDL <MDL MDL
174 00150 384 00414 162 00172
IVA MDL <MDL
PACT-M-2.0 Eacd V an ce TO
TOX-120-Uvcr.xb
tnwn
5:12 PM
000461
1 Fkrr-TVai-120 ' 2ft-Diy Oral Tox Stody by Daily Gavas la Rats
FiodKtN aaboCftrt Sabetncc): Matrix: M cd w iM M Aanlyffcat EqufAcat SyOeaNoaba:
Dateof EatactkMAoriyat: Dale o f AulyMMMlyiC DateofDate ftefectm'Aaelyat:
S ia p te D a ta
TOX1R Piptooney 2t-DeyOnJw k ity te d y fcyDxflyGavageh l t
T-7Q23..T-70HT-7022,T.7032, T-6293 Rat liv er FCT-U-1.0A F CT-M-2.0 Madeihe 041099
Mm L M 04/1*99 RWW/7CMAH 04/20/99,0S/21/99,05/25/99 HQI/DRB/KJH7
s/oso* 04/9* w w meetcwh;
R-SqeiradValac: Slop
RAT LIVER Grasp te l
Ceoc.
U OtedUk
Matrix Blk
-JM H* Q C -250H *
Q C -2*
Crw p 4 H O m fkJd a y
Creep 4 ) 0 .0 a |t|%
ItooHii 1-14Dm
C reap4 30.0sftU*y Recovery 2* 2t D m
CreopS lO.OBgftg/dajr
ma
C rM ,S 10.0o p t9**T R oovor 1 14D m
GroopS l* 0 fftfttap Recovery 2- 29 D m
C reep X0mfk$Sty
rros
Creep 6
M ^W tgr
Reeevov 1 H D m GreepO
J.O m fltVtr R ccevay2-2ID m
H20 Btt*t
H2Bft~2 R ateU verB tel RebUlUvcr Btt-2
3M445 3M-MSD
AUB KMSD 13M-MS 13M-MSD
fU SOM SIM 52M S3M 54M
SSM SOM S7M SSM S9M OIM C2M CM
45M 66M
C7M M 09M 70M
7IM 72M
73M 74M
7SM 77M 7IM 79M
I1M 92M <3M S4M
SM 6M I7M
IIM WM
90M 91M 93M MM 95M
0 *99
374.4500 310.0600 4001000 390.4100 379.4300 311.7500
NR NR HR
NR NR NR NR NR NR NR NR
NR NR NR
21.) 73.
Nr
NR NR
NR NR NR
154 9*3 903
ii)
IM
m
24*9000 5001700 341.9500
429.1100 49*0600 4*9.7200
455.7600 403J500
32*9700 330.4600
26U200 209.1100 354.0S00 251.4300
MatedDcm(miUsa(ftffig: nOS-S*3*fcrOAA-2S*fc
p ros
p fo
MMIm
C atcC eac
M ar
tfe
<MDC
1 MDL
1 MDL
1 <MDL
1 27409%
1 21575%
1 31931%
I 30793%
i 21104%
1 29090%
VALUE!
1 VALUEI
1 VALUE!
t VALUE!
I VALUE!
1 VALUEI
1 VALUE!
1 VALUE!
1 VALUE!
1 VALUE!
1 VALUE!
1 VALUEI
1 VALUE!
1 VALUE!
1 J
1 731
1 VALUE!
1 VALUE!
t VALUE!
t VALUE!
1 VALUE!
1 VALUE!
1 153
1 9U
1 90.
t 117
i IM
t 1*0
1000 245630
1000 S0271C
1000 . 352337
1000 / 433347
1000 .
47(539
1000 49549
1000 456739
1000 400571
1000 5553
1000 332625
1000 27002*9
1000
1000 *
20790*7 253062.9
1000 250597.1
DateB attit^Aaaiyst: DeteVcrified/Aub*:
<0/24/99, 03/1*99,<0/17/90,03/22/9. 05/24/9*05/24/99 LAC 06/01/9,06M 99 EAD
Average O S
rt
<MDL <MDL 94% 105% 97%
NR NR NR
*0509 *114 *1053
419.4 3091 2371
USD SM. Dey.
<MDL <MDL
4% 4% *0
*0 NR
*0
NR *0 NR
2 *0317 301 *0344 2*9 *0261
2*44 95.73 lt.0 6 3*19 1*7 254
' rOAA Cale. Ceoc.
rt
<MDL <MDL
<MDL <MDL
254 260
299.93 31030 344.99 312.71
VLUfcl
VALUE! VALUE!
VALUE! VALUE! VALUE! VALUS!
VALUE! VALUE!
VALUE! VALUE! VAJfel VALUE! VALUE!
*00 *00
VALUE! VALU VALUE! VALUE!
VALUE! VALUE!
*00 *00
VALUE! *00 0.00 0.00
VALUE! VALU
VALU
VALUE! VALU
VALU VALU VALU VALUS
VALU VALU VALU VALU VALU
fetal A m w t fPOAA
<MDL <MOL <MDL <MDL 5% 97% 100% 104% * 115% 105%
NA
NR NR NX NR NR NR NR NR NR NR
Mr
NR NR
*00 *00 NR NR NR NR NR NR *00 *00 NR *00 *00 *00
Mr
NR NR NR NR NR NR NR NR NR NR NR NR NR
W A -N otA ppteaU e FFOS-PaftwoocteM FOA A "
m fS-tafte
Average FOAA rt <MDL <MDL 9% 102% 110%
NR NR NR
*00 *00 *00
NR NR NR
D SM.te * .
MDL <MDL *0232 *0340 * *0992
*00 NR *00 - NR *00 NR
DIV/M *00
DIV/M *00
IXV/H *00
*00 NR *00 NR *00 NR
te VALU VALUS VALU VALU VALU VALU VALU VALU VALU VALUS
4ot)
2433 2447 2703 903 2034 4347
5962 169
3*4 191 25.0
40J 1*3
VALU VALU
VALU VALU VALUS
VALU VALU VALU
VALU VALU VALU VALU VALU
VALU
Va l
VALU VALUS
VALU valu
VALU VALUE!
VALU VALUE!
VALU VALU VALU VALU VALU
______fe
NR NR NR NR NR NR
NR NR
Mi
NR
4.o
2.43 *43 2.70 *103
*03 415 SK
*169
*0294 *191 *0250 *0405 .0,0103
rat
NR NR NR NR NR NR NR NR
NR NR NR NR NR
NR
NR NR NR NR
rat
NR NR NR
NR NR
Mk
NR
rat
Average Ma NR NR NR NR NX
SD 8M.De*.
NR NR NR NR NR
*09 *12 0.0253
S tJ 119
6*
*0940
5*7 *0151
0 NR NR
*09 NR NR
*00
rat NR
*00 NR NR
*00 NR NR
*00 NR NR
TOX-lW -tlvcrxli
0/1099 5:12 FM
000462
FACT-TOX-120 28-Day Oral Tox Stndy by Daily Gavage la Rats
Sudy: frodart Ih rta r SrtMce): Marfz: . MdfcodflUvfafaa:
Date o f ExOactioaMaatyil: D tfetfA adpbftailytf: ranflT ii ftintniinafftaityir
S a a p k D ata
TOX-I2R ExptortdTOX-121 Eaptamocy2-DayOralTaxkfy Sudyby DailyGavageJ*Rata
'T-7B23,T`7024, T-7D-7023, T-7024, T-7Q22, T-70J2, T-4295
R atL tar
lU ttirar
Sefiat lerigt*
FACT4<1.0A FA<FACTM>1.0k PACT-M-IO
R-Sund Vatw;Se*ABaefanoas
H atrtinr 0*1034 M aidbc 0*1094
Mw Lj b M Mm L}d 3 J
04/1V99 RWW0CD4/HW9 RWW/3CP/SAH
04/2099. CcS/21/ML0V2D9R 03/21/99,05/25/99 HOJVDRMUH?
05/05/99,05/24/99,05/05/99,05/24/99, 05/24/99 MEEflUH/HQI
RATUVER Craap 9m
MahodBlk M rtriiBlk
Q C -2pf* o c-:5 0 pp.
Q C-SOp*
C napl A ta |/k |U q r
G rw p4 H O afftpttqr K tttw y 1 14D m
GrM ?4 tt.O H fif'd ty Rceovcry 2 - 21 D m
Cr* S IlO afftftfap
FFHS
GreapS ie.O B f/kfttajr te a v a r 1-14 D m
Cfw pS lt.0 a |lk # lq r R*C9very2-2lDm
m p^ 10*l[*tfy
FPOS
C r*M Zacevov 1 14D m
Cmpl
Xecovcry2 -2S D m
Ifp ltl
tOOBIk-I H20 Blk-2 RabMUyerBTT RaMtUverBlk-2
3M44S 3M-MSD M 44S SM*MSD u m -m s UM-MSD
49M 90M SIM 52M S3M 94M 55M 94M 57M SIM 59M IM 42M
O4HM
4M 47M 404 49M 70M 7IM 72M 73M 74M 7SM 77M 704 79M
iU
42M DM
Mt$MM
UH 47M MM 9M . 90M 91M 93M 4M 9SM
PFOS CMC.
*M
0 0 194 374.4500 2460400 4005000
390.4400 75355 391.7300
Vk
HR NR NR NR rat NR
NR NR NR
NR NR
NR
NRJ
73.9
NR
NR NR
Mt
NR NR 194 963 90.3
117 414 133
2469000 5012700
3411500 4211400 492.0600 499.7200 455.7400 403.3500
355585
3314400 2413200 209.1100 254.0900 251.4300
PFHS Cale. Cmc.
tfe MDL
MDL MDL <MDL
273 254 319 311 29.4i
29154
fVALUEl
VALUS fVALUEl OVALUEI
9VALUBI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI
OVALUEI OVALUEI
OVALUEI OVALUEI
mu
359107 243354
337434 345414
314441
223309 347000 49362 44349 47999
37903
42744 24199
fVALUEl OVALUEI VALUS OVALUEI
OVALUEI OVALUEI OVALUEI OVALUEI VALU OVALUEI OVALUEI
ovaLi
OVALUEI OVALUEI
HrttoJTVirrrtna1Mil(MDL): PPOS-SA3aa'*90AA-25art.P
TataJ AjMat
efPFHS
/k MDL
<MDL <MDL
MDL
9m 15%
107% 104% 19%
97%
NR NR NR NR NR NR NR NR NR
NR NR NR NR NR
340
359
243
334 344 316
223 347 . 49.4
414 410
37J
421124 Ml
NR NR NR NR NR NR NR NR NR NR }*R NR NR
A verti* PFHS tft MDL <MDL 19% 109% 93%
NR NR NR
327 61.9 343
NR NR NR
Detc Eaiered/Aaely: D*UVoilkd/AaaijM:
02/24/99,WW,ovnm, tamm,as
04/0499.069999 EAD
RSD StlD ev. <MDL MDL 10723 10237 10930
NR NR NR
13.9 510 19.0 11.7 314 7.42
NR NR NR
M513 CafeCaec.
O i <MDL
<MDL MDL <MDL
225 227 273.16 334.12 217.43 247.44
fVALUEl
OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI
OVALUEI OVALUEI OVALUEI
oVaU OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI
OVALUEI OVALUEI
OVALUEI OVALUEI OVALUEI
OVALUEI
OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI
oVALUfi
OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI
TM IAm u I f M5U
a'i <HDL MDL <MDL
MDL 75% 74% ' .91% 112% 73% 90%
U&
NR NR NR NR NR
NR NR NR NR
NR NR
NR NR
NR NR NR
NR NR
NR NR NR NR
NR NR
NR NR
NkR
NR
NR NR NR
NR NR NR NR NR NR NR NR NR
Avcraga M513 4fr MDL MDL 70% 102% 41%
NR NR NR
NR NR NR
NR NR NR
N/A-NMApptfcaMa
FFOS*FcrfhBiooetne 70AA-
USD 4(4. Dev. MDL MDL
m 30% 131
NR NR NR
NR NR NR
NR NR NR
M413 Cale. CMC
rt MDL
MDL MDL MDL
241 229 147 313 325.91 23171
fVALUEl OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI OVALUEI VALUS VALUS VALUS VALUS VALUE! VALUS VALUS VALUS VALUS VALU VALUE! VALUS VALUS
VALUS VALUS VALUS VALUS VALUS VALUS VALUS
VALUS VALUS VALUS VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS
Ti M Am u I
' MM413
afe
<MDL
<MDL
<MDL
MDL
4t%
77%
43%
71%
74%
71%
ta
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
kR
NR
`
NR ft
NR
NR
NR
NR
NR
NR
NR
NR
.NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NX
NR
NR
NR
NR
NR
Average M413 MDL MDL 79% 47% 77%
NR NR NR
NR NR NR
NR NR NR
RSD SM.DCV. MDL <MDL
9% 13% 103
NR NR NR
rat
NR NR
NR NR NR
FAC-M -2.0 E u d Venta* 7.0
TOXI20-tivcrjs
4/1099
9:12 PM
3M Environmental Lab *Fluorine Analytical Chemistry Team
P F S 's
PFHS perfluorohexanesulfonate
C6F13S03-
PFOS perfluorooctanesulfonate
C8F17S03-
PFOSA
C8F17S02NH2
PFOSAA
C8F17S02N((CH2CH3)(CH2C00))-
M556
. C8F17S02N((H)(CH2C00))-
M570
C8F17S02N((CH3)(CH2C00))-
PFOSEA perfluorooctanesulfonylethyl amide C8F17S02N((CH2CH3)(H))
Et-FOSE-OH PFDS perfluorodecanesulfonate
C8F17S02N((CH2CH3)(CH2CH20H)) C10F21SO3-**
PFCA*s POAA M513 M613 M463 M563 M363
perfluorooctanoate perfluorodecanoate perfluorododecanoate
C7F15COOC9F19COOC11F23COOC8F17COO- ** C10F21COO- ** C6F13COO- **
PFS = perfluorinated sulfoante PFCA = perfluorinated carboxylic add
** no standard material available for confirmation;
000463
3M Confidential - Attomey/Client Work Product Privileged - D o N ot Disclose
PFHS in M ale and Fem ale R at Liver, Group 5 3M Environmental Lab - Fluorine Analytical Chemistry
co
! i
0 "5
PFOS In M ale and Fem ale R at Liver, Group 6 3M Environmental Lab - Fluorine Analytical Chemistry
UU0464
POAA in Male and Fem ale R at Liver, Group 2 3M Environmental Lab - Fluorine Analytical Chemistry
1 14 28 Day
10
9
6o
7?
6
t5 S E
4o
3
a w
2S
1
0
M513 in Male and Female R at Liver, Group 2 3M Environmental Lab - Fluorine Analytical Chemistry
Team, Project: FACT-TOX120
3
Ie 2.5 2I
8
c
<3
1.5
o
I
1*
0.5S
4
/ft
1 14 28 Day
M613 in Male and Female R at Liver, Group 2 3M Environmental Lab - Fluorine Analytical Chemistry
Day
0.16 0.14 0.121 0.1 I 0.08 0.06 0
0 .0 421s 0.02 0
00465
D 000466
000467
TOX-120,35M G3 060899009
100-,
M613 (C11EZ3CQCE)1
3: MRM o f 1 Channel ES5.85 TIC
1.09e3
%
0.69.Q.75
23 ` v * 0650899009
lOO-i
1.72^2.13^ \2 - 5 8 \3 -3 1 3.85 4-^ 4 .8 8 f ' \ 5,59^ 1
6.06
|M63 (C1UKZ100CE]|
5.72
6.89 7.7U 7.86 / r m
6:i^RM o f ihannel ESTIC 559
%
0 38 1 03 1-47i^^.11,.5599
3 77
536
22..6655 33..0099 33..55.3S /s
44..8866 55..2255.. |
54 11i*i 11111f i i*i
-,06089900? 100
^ 1^ 11 hfyfa*
,W | 4 , , r , u v ^
|M513 (C9h l9C O O ^
6.75 7.52_ 8 .14.8.29 ,, 7.02 \ .^ 1 C s '? '61
2: MRM o f 1 Channel ESTIC
8.68e3
%.
060899009
100-,
' 'i t t 1 1 1 1 1 i 1 i , - i 1 1 i i i i 1 i i 1 1 1 i 1 rv~[~
|M36'3"(C8FI'KXKFJj
%:
1 1 1 1 1 1 j 1 1 r i i"T 1 1 1 j '1r,,v i "i r 1 1 I 1 1 1 1-
5: MRM o f 1 Channel ES TIC
6.65e3
060899004' " '
100-,
%-
(PO A A fC 7H 5C O O -l
Tn ' ' 111M H l"r" 5.14
1: M R M o f 1 C hannel EST IC
1 .5 6 e 5
m "t r f r r i r1 1
060899009
100-,
1
................................................................ ..C h a n n e l E S -
M3&3 (C6E13UUU-)|
TIC 489
0.66 1-08 1-64
8.93 Time
000468
000469
000471
000472
; Environmental Laboratory O m RequestForm . .
3M Environmental Laboratory 935 Bush Ave. Building 2-3E-09-
St. Paul, MN 55106
Attn: Sample Custodian Phone (612) 778-6750 Fax (612) 778-6176
SafelyConcerns/SpecialHandl ing. [P.rmpm . ., v / V
jagafeas^
>>*1Cffl^i*VSri* WWfeKgj
:' f-A
/Sample No. (Range 0-999)'
[llab AequestNo.'
I
I Data Received
PlojaaLaad----------------- Expected Completion Data
k in Planning Status
O D /n x /n n
Quality Review *
10
Class/Pflority
Date Shipped
1M&.IPaSSfe
>: T^ J 'i-ii, -
is s m .
,0 3 /
/ :/V/
...
'' .:?***'?*
.. . . -.
-V--v--. -" ;-------------'-.--:-
VC--.'.. /
sg i
', > *.'. v.* v . r " --"J *. , hi *" ,`k.r *
. .. ' ...... .
V ' ^ *.
-a v :'
.V;5; . T~ " : . r^ `. " :.;v; i`?.>:;-/V;''T `." * :..>.\.;'
.'. r . I.'.
V*
tt''
I 'JW
`^ -!y -;^ T --J/;/yJ-V*1/ CV: '!V' -
v i * - . p - . - .-
*
--- .'*:t;
r.", .'i.' ./'.'.A'/lv'-V/'-''.-.'. '-vA-fj' .
. ' / .f
.. i. , . .
. , - - ' .
. r.-: K v : .
j;.r .;
. -.V c
v`
' :
v .t*...
;... y .
' ------ :-------------i,--
Total Container^ of Each Item O
000473
C hain of C ustody R ecord
3M Environm ental Laboratory
b v '
Forni 35176-PWO 3M Facility
,
Address
3 /0
::
'1 '
,. . 1 .`j i-
,1. 1 `
T n y i f n l 0Ci\\
vJJ
'
, ' ' 1;.. .
City. State. Zip Code
_________ S\. P f i J
,,h : - ,ff| lHlG MA) S S M - ..
y bi
V lQ 7 lW ' U O ( -L b
3M Requester _ f . . . . . . . .
va V .
'* ,
rlO n /in
3M Project Lead
- A ' ' ( .<;/>
` i vV; : t
' ' ' 1.
. : . ' 3MProJectTitle ,
,.
|.
JOTnv .0W :Km TA X ISO ,).?/>.. Ar/.l TDtf P f i
3M Facility Contact . '
U
:,:i; : HiM.-.* r j u *:V ' ' n .*'****': ' '-i..*..? 0`i? Hi
3MTelephone Number
3M'ProJect Number.
, .. .
ie.JPz.4i
/ Rocu^nn
3M Department Numtier
: qa< ' ' ' -
Lab Requesl/Template Number
________________ . 7 3 V - S I n
.
____
SampledBy(Print) SamplerSignature
V IA ''
"
Contractor<,/..if.f..w...f..1../.,....*......;..., :1
tin r x
Dele
Number Containers -, of each
Preservatives
f ( 3 K Sam ples S p lit See A ttachm ent : Comments
Item #
K) A '!. - ' . I
Sample Description
.
!S A w /W Date Time Matrix
8i
Mi
i
N
Xz
t' X 9z
i i tlf- ' 1
G lA ^ ^ o O ^ (!iiiiT i) ! VniliVilc.!rl u p n n " ' i r i m ' p i f i * rPTfijl) S f l t l- 0 c iv Fil S j } f f tsp in l i t i ' 1' 4tSr`
1
f,,F I
2
G ro2
L iO A r L ii/tr
18
, t ,* t' '
t* iO l U jO f.H. f : .
w:
iler-iLfr irt-Tortna v-v,
I.i-.I
.
34 1 4
.i L-:Ji!
1 \rtr L ivtr
1 .
t t r-liili 9 l i L'il i>;
ir
1
M h ih s -
5 -C xji 6
(I r p L
Lii/vr Ln/-rr
18 If"
1;.IO j h iIC 'iri'.:!
.j ' i
7 ....... ________________________ -- ------------------ -- -------------------------- -- T
8
9
. . . * ' 1 '
* **
10
Vi /
4 i : . i-H i ' t . * *
11
12
13 -- :----------------- ~
------- :-------- -------------------------------------- ------------
Item #
Relinquished By/Affiliation
Time
Date
Shipped Via
Received By/Affiliation
Time
Date
J r Bvprt-M . lM - J __CJh a m *____ 2 L m j L 0 2 l n ? h
f'l: !:' ;Oin
I a lli. fi ui
BESTCOPjTCMIWjLr
Original - Accompanying Samples
Last Page Orlgnalor
See Reverse Side For instructions
Total Containers of Each Item
000474
C hain o f C ustody R ecord
3M Environmental Laboratory
Forni 35178-PWO 3M Facility
3 ft) Tovrc.^locioi
Address
(J O
3 2 -3 .E -0 1
City, Stale. Zip Cod
_____ S i, Paul, MM S S I3 3
nq71Pf U o i J, 0
SMRequeKer 3M ProjectLead
M ri/n C .& s*.
3Mpro`#c,Ti"#
..
__________ K r i Hn5*____________________
T W
. 3M Project Number
WOToy
3M Fadty Contact
TfcXiao 2SCV 5 rJ *n>y YPSs I r R ii+j R V qaqiiopo/B U ^ qi^o 3M Department Number
Mctrvh C a s e ,
3M Telephone Number
HM l
Lab Requeat/Template Number
_________ __ ______________ 7 3 3 - S I ; ) ________________________________________
Sampled By (Print)
Sampler Signature
Item # 1 TGir-I 2 G rp 3. 3 4f
& p*t
5 6 Grp L
7 8 9
V /A .
h )lA Sample
Description
Contractor
A )0T O *
Dato
ll/o lM - I3 I r t h s Date Time Matrix
L iter Lit/tr / .itor L lW LiVer Liw r _4r--
Number Containers of each
Preservatives
2
n O
Z
8M
X
XX
Sam ples S p lit See Attachm ent Comments
(jnA\h AV 1 IfVnviii nlt/foyx iJhhn ^Arrin/i>
tRr/jpV S i t 38 r
28 28
S p ttim e n Irsi 4t)r r n*HtrrWr .motormff1h,hK
Ur.
p o ifo q
at .
------------ ;----------------------- -- ------------------------------------------
*
10 / 11
*w
12 13 --
Item #
UL
Relinquished By/Afflliation
Time
Date
Shipped Via
Received By/Affiliation
k X ^ n e . B t f n j i f i h k C JjL nv* 7T-
' -'i;
Time h.
Date
t i v i * v y
t aetPana-rinnator
,r\
' ' . - f~!
' I. r> f ? : * > n< , f \'
See Reverse Side For Instructions
000475
11/09 'a 18:24 FAX 01736418343
NOTOX B.V.
@003/017
1
I
]
3 PROTOCOL
1 I
j EXPLORATORY 28-DAY ORAL TOXICITY STUDY
' WITH
|
TELOMER ALCOHOL, TELOMER ACRYLATE,
PFHS AMD PFOS (positive control)
! BY DAILY GAVAGE
| IN THE RAT
* FOLLOWED BY A 14/28-DAY RECOVERY PERIOD
J
J NOTOX Project 242933 NOTOX Substances 83484, 83493. 83502, 84456 and 84141
j 3M Test No. T-7023. T-7024, T-7022. T-7032, T-6295
j - page 1 of 16 -
J
000476
t
11/09 *98 16:24 FAX 31736418543
NOTOX B.V.
0004/017
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
NOTOX Project 242933
l 3M Test No. T-7023, T-7024
I T-7022, T-7032, T-6295
! PROTOCOL APPROVAL
1 ----------- :------5------- 1-------------------
STUDY DIRECTOR :
1
Ir. S.C.M. de Hoog
. Date: 11 JELopfcorn-bOe 1 Q 0 ( 9
| SPONSOR:
J
1
J
]
Marvin T. Case, DVM, PhD Corporate Scientist SM Toxicology Services
D*t,: iv J ti iU * I f f f .
L. c 'I
* Page 2 -
000477
11/09 '95 18:25 FAX 31738413543
NOTOX B . V .
1
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
0005/017
NOTOX Project 242933
1 PREFACE ] Sponsor 1 Study Monitor 1
Invoice Contact
Testing Facility
Study Director
J Pathology
1 ]
SM Toxicology Services 3M Center, Bldg 220-2E-02 St. Paul. MN 55144-1000 USA Marvin T. Case, DVM, PhD 3M Toxicology Services 3M Center, Bldg 220-2E-02 Telephone (651)- 733-5180 Fax (651)- 733-1773 Barbara Nelson 3M Procurement Operations 3M Center. Bldg 224-1N-04 St. Paul. MN 55144-1000 Telephone (651)- 733-0766 Fax (651)- 733-1799 NOTOX B.V. Hambakenweterlng 3 5231 DD 's-Hertogenbosch The Netherlands Ir. S.C.M. de Hoog Dr. J. Th. Wilson
.V
- Page 3 -
000478
11/09 '96 16:25 FAI 31736416543
NOTO! B . V .
1____________________________________________ ____
1
! TELOKER ALCOHOL/TELOHERACRYLATE/ /PFHS/PFOS
0008/017
NOTOXProject 242933
STUDY PLAN
1 Start of Treatment
] 06 October 1998 (males)' 08 October 1998 (females) Start Recovery
1 04 November 1998 (males) 06 November 1998 (females)
Blood sampling
1 03 November 1998 (Main groups, males) 04 November 1998 (Main and Recovery groups 1 and 2, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main and Recovery groups 1 and 2, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females)
Urine collection
predose
29*30 September 1998 (Recovery groups 1 and 2)
day 7
11-12 October 1998 (Recovery groups 1 and 2, males)
day 7
13-14 October 1998 (Recovery groups 1 and 2, females)
J day 14
18-19 October 1998 (Recovery groups 1 and 2, males)
day 14
; 20*21 October 1998 (Recovery groups 1 and 2, females)
end of treatment : 03*04 November 1998 (Recovery groups 1 and 2, males)
end of treatment : 05*06 November 1998 (Recovery groups 1 and 2, females)
end of recovery : 17-18 November 1998 (Recovery groups l, males)
] end of recovery end of recovery
19-20 November 1998 (Recovery groups 1, females) 01-02 December 1998 (Recovery groups 2, males)
end of recovery 03*04 December 1998 (Recovery grdups 2, females)
] Necropsy 03 November 1998 (Main groups, males) 04 November 1998 (Main groups, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main groups, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females)
Draft Report
] 26 March 1999
I 000479 Pag 4 -
11/09 '98 18:25 FAX 31736418543
l
NOTO* B . V.
0007/017
TELOMER ALCOHOL/TELOWR ACRYLATE/ /PFHS/PFOS
1
NOTOX Project 242933
TEST SUBSTANCES
The sponsor 1s responsible for the completeness of all test substance data. All available Information on characteristics of the test substance will be taken
Into account before start of the study 1n order to determine 1f they conflict
w1th/m1ght affect the proceeding of the study.
] NOTOX SUBSTANCE 83484
(data will be added by protocol amendment)
Identification
TELOMER ALCOHOL (T-7023)
1 Description Batch
Purity
Test substance storage
1 Stability under storage conditions
Stable
Expiry date Stability in vehicle
1% aqueous carboxymethyl cellulose: not indicated
r Safety precautions
Corn oil: not Indicated Gloves, goggles and face mask to ensure personnel
health and safety
Disposal Category
NOTOX SUBSTANCE 83493
(data will be added by protocol amendment)
Identification
TELOMER ACRYLATE (T-7024)
Description
Batch
J Purity Test substance storage Stability under storage
conditions
Stable
3 Expiry date Stability 1n vehicle
1% aqueous carboxymethyl cellulose: not indicated Corn oil: not Indicated .x
Safety precautions
Gloves, goggles and face mask to ensure personnel
health and safety
] Disposal Category
J
- Page 5 -
000480
11/09 '99 16:28 FAX 31736418543
NOTOX B.V.
@008/017
TELOMER ALCOHOL/TELOMER ACRYLATE/' /PFHS/PFOS
NOTOX Project 242933
NOTOX SUBSTANCE 83502 (data will be added by protocol amendment)
] Identification Description Batch
(T-7022)
Purity
] Test substance storage Stability under storage conditions
Stable
Expiry date
] Stability 1n vehicle Safety precautions
1% aqueous carboxymethyl cellulose: not Indicated Corn oil: not Indicated Gloves, goggles and faee mask to ensure personnel
health and safety
Disposal Category
NOTOX SUBSTANCE 84456 (data will be added by protocol amendment)
Identification
PFHS (T-7032)
Description
Batch
Purity
Test substance storage
Stability under storage
conditions
Stable
Expiry date
Stability 1n vehicle
1% aqueous carboxymethyl cellulose: not Indicated
Corn oil: not indicated
Safety precautions
Gloves, goggles and face mask to ensure personnel
J Disposal Category
health and safety
1 NOTOX SUBSTANCE 84141 (data will be added by protocol amendment)
Identification
PFOS (T-62P5)
J Description Batch Purity
Test substance storage
Stability under storage
conditions
Stable
Expiry date
Stability in vehicle
1% aqueous carboxymethyl cellulose: not Indicated
Corn oil: not Indicated
Safety precautions
Gloves, goggles and face mask to ensure personnel
health and safety
Disposal Category
] Vehicle
1% aqueous carboxymethyl cellulose or Corn oil, specific gravity 0.92
Rationale for vehicle Stability 1n vehicle
Based on trial formulations performed at NOTOX Stability over 4 hours will be determined by the *
sponsor - Page 6 -
0C0481
L___:________ ,_________ __11/09 *93 18:26 FA* 31736418543
NOTO! B.V.
1
, TELONER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
009/017
NOTOX Project 242933
TEST SUBSTANCE FORMULATION
Method Storage conditions
Formulations (w/w) will be prepared dally within 4 hours prior to dosing. Zf necessary, adjustment will be made for specific gravity of vehicle. At ambient temperature
No chemical analysis of dose preparations will be performed.
PURPOSE AND RATIONALE
The nature and purpose of this exploratory study will be to determine and compare the relative oral toxicity in rats following oral (gastric gavage) doses administered daily over 28 days and identify developmental compounds of lesser toxicity for commercial development. This study should provide part of a rational basis for toxicological risk assessment 1n man. The oral route was selected as 1t 1s a possible route of human exposure during manufacture, handling or use of the test substance.
GUIDELINES
This protocol was reviewed and agreed by the Article 14-functionary and the Ethical Committee of NOTOX as required by the Dutch Act on Animal Experimentation. The study procedures described 1n this protocol will be based on the following guidelines: 1) EC Directive 96/S4/EEC, Annex IV.D. replacing EEC Directive 67/548/EEC, Part B : Methods for the Determination of Toxicity and other"Health Effects; B.7: 'Repeated Dose (28 days) Toxicity (oral)". Official Journal of the European Communities -No. L248, September 1996. 2) OECD Guideline for Testing of Chemicals, Guideline 407: 'Repeated Dose 28-day Oral Toxicity Study 1n Rodents', Paris Cedex, 27 July 1995
This protocol, study procedures and the report will not be Inspected by the NOTOX Quality Assurance Unit.
ARCHIVING
NOTOX B.V.. will archive the following data for at least 10 years: protocol, raw data, report and all specimens. No data will be withdrawn without the sponsor's written consent.
- Page 7 -
000482
11/08 '98 18:27 FAI 31738418543
NOTO! B.V.
1 _______________________________ ___
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
I
010/017
NOTOX Project 242933
TEST SYSTEM
] Test System ]
Sprague Cawley rat Cr1:CD BR, outbred, SPF quality. Recognised by International guidelines as the recommended test system (e.g. EPA, FDA, OECD, EEC). Females will be nulUparous and non-pregnant. Source : Charles River, Sulzfeld, Germany
Age at Start of Treatment Approximately 6 weeks
1 Number of animals
84 males, 84 females
Randomisation
1
At least 5 days before study start, by computergenerated random algorithm according to body weight, with all animals within 20% of the sex mean. A health inspection will be performed prior to commencement of treatment to ensure that the animals are 1n a good state of health.
! Identification
Earmark and tattoo
ALLOCATION
] ]
Group Identification
Test Substance
Number of animals Animal numbers
males females
males females
J 1 Main group
Vehicle control
1 Recovery group 1 Vehicle control
1 Recovery group 2 Vehicle control
88 33 33
1- 8 ! 97-104 9-11 105-107 13-15 109-111
2 Main group
Telomer Alcohol
88
17-24 113-120
2 Recovery group 1 Telomer Alcohol
33
25-27 121-123
1 2 Recovery group 2 Telomer Alcohol
3 Main group
Telomer Acrylate
33 8
29-31 125-127 33-40 129-136
3 Recovery group-1 Telomer Acrylate
33
41-43 137-139
3 Recovery .group 2 Telomer Acrylate
33
45-47 141-143
] 4 Main group 4 Recovery group 1
88 33
49-56 145-152 57-59 153-155
4 Recovery group 2
33
61-63 157-159
5 Main group
PFHS
88
65-72 161-168
5 Recovery group 1 PFHS.
33
73-75 169-171
5 Recovery group 2 PFHS
3. 3
77-79 173-175
6 Main group
PFOS (positive control) 8
8
81-88 177-184
! i
6 Recovery group 1 PFOS (positive control) 3 6 Recovery group 2 PFOS (positive control) 3
3 3
89-91 185-187 93-95 189-191
I Test substances Telomer Alcohol (substance 83484), Telomer Acrylate (substance
(
83493) and
(substance 83502) to be tested at a dose level of 30 mg/kg body
1 weight/day.
PFHS (substance 84456) to be tested at a dose level of io mg/kg body welght/day. PFOS (substance 84141) to be tested at a dose level of 3 mg/kg body welght/day.
- Page 8 -
000483
11/09 '93 18:27 Fa * 31738418543
1
NOTO! B . V.
TELOMER ALCOHOLfTELOHER ACRYLATE/ /PFHS/PFOS
0011/017
NOTOX Project 242933
ANIMAL HUSBANDRY
Conditions Air-conditioned room with approximately 15 air changes per hour and controlled environment with optimal Conditions being a temperature of 21 3*C and a relative humidity of 30*70%. Lighting will be 12 hours artificial fluorescent light and 12 hours dark per day. Accommodation Group housing of 3*4 animals per sex per cage 1n stainless steel suspended cages with wire mesh floors. Acclimatisation period 1s at least 5 days before start of treatment under laboratory conditions. Diet Free access to standard pelleted laboratory animal diet (from Carfll Quality BVBAr.Oud-Turnhout, Belgium). Each batch will be analysed for nutrients and contaminants are analysed on a regular basis. Results are examined and archived. Water Free access to tap-water. Certificates of analysis (performed quarterly) are examined and archived.
TREATMENT
Method Frequency Dose volume Dose level
Oral gavage, using a stainless steel stomach tube.
Formulations will be placed on a magnetic stirrer
during dosing.
f
Once dally for at least 28 days, approximately the same time each day, 7 days per week. Main group animals are dosed up to the day prior to necropsy.
5 ml/kg body weight. Actual dose volumes will be calculated weekly according to the latest body weight.
Group 1: 0 mg/kg b.w./day (Vehicle Control) Group 2: 30 mg/kg b.w./day (Telomer Alcohol) Group 3: 30 mg/kg b.w./day (Telomer Acrylate) Group 4: 30 mg/kg b.w./day Group 5: 10 mg/kg b.w./day (PFHS) Group 6: 3 mg/kg b.w./day (PFOS, positive control)
RECOVERY
Duration
Recovery group 1: at least 14 days Recovery group 2: at least 28 days
i-
- Page 9 -
000484
1 1 / 0 9 '9 8 1 8 : 2 8 F A X 31738418543
NOTO! B.V.
1 _____________________
I
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
0012/017
NOTOX Project 242933
OBSERVATIONS
1 Mortality / Viability
3 Clinical signs
1
1
Body Weights
]. Food consumption Water consumption
Twice dally. Animals showing pain, distress or discomfort, which 1s considered not transient 1n nature or 1s likely to become more severe, w111.be sacrificed for humane reasons. The time of death will be recorded as precisely as possible. Twice dally, detailed clinical observations will be made 1n all animals: once Immediately after dosing and once approximately 4 hours after dosing. The time of onset, degree and duration will be recorded. All symptoms will be recorded and graded according to fixed scales (specified 1n the report). Treatment period : on days 1, 5, 8, 12, 15, 19. 22, 25 and 28. Recovery period : on days l, 5, 8, 12, 14, 19, 22, 25 and 28. Weekly. Subjective appraisal will be maintained during the study and a quantitative assessment Introduced 1n the event of a suspected treatment related effect.
CLINICAL LABORATORY INVESTIGATIONS (Main and Recovery group animals) t
J Blood samples will be collected under light ether anaesthesia, between 7.30 and 9.30 a.m. Immediately prior to post mortem examination for Main and Recovery group animals, and for Recovery group animals also at the end of the treatment
3 period. The animals will be fasted overnight (with a maximum of 20 hours) before blood sampling, but water will be provided. Blood samples will be drawn from the retro-orbital sinus of all Main and Recovery rats/sex/group and collected Into tubes prepared with EDTA for haematologlcal parameters (0.6 ml), with citrate
] for clotting tests (1.0 ml) and untreated tubes for clinical biochemistry parameters (>2.0 ml).
!
- Page 10 -
000485
11/09 '98 16:28 FAX 31736418543
1l"... .
.......... .
NOTOX B.V.
---- --
013/017
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS 1
NOTOX Project 242933
! HAEMATOLOGY
1 The following haematology parameters will be determined from blood prepared with EDTA as an anti-coagulant, using the Instruments listed:
Parameter
Unit
]
Erythrocyte count
T/l
Instrumentation Sysmex K-1000
1 Haemoglobin Haematocrlt
mmol/I 1/1
Sysmex K-1000 'Sysmex K-1000
1 Mean corpuscular volume
fl
Sysmex K-1000.
Mean corpuscular haemoglobin
fmol
Sysmex K-1000
Mean corpuscular
mmol/1
haemoglobin concen
i tration
Sysmex K-1000
*
Platelet count
G/1
Sysmex K-1000
Red cell distribution
width
%
Sysmex K-1000
Total leucocyte count G/1
J Differential leucocyte l(rel) count
Sysmex K-1000 Manual (Microscope)
I The following haematology -parameters will be determined, from blood prepared with citrate as an anti-coagulant, using the Instruments listed:
3 Parameter
Unit
Prothrombin time
sec
Instrumentation Sysmex CA-5000
Partial thromboplastin
time
sec
Sysmex CA-5000
j
Page 11 -
000486
11/09 '98 16:29 FAX 31738413543
1 1
--
- - ' --
NOTO* B.V.
014/017
1
Telomer alcohol/TELOMER ACRYLATE/ /PFHS/PFOS
1
NOTOX Project 242933
I CLINICAL BIOCHEMISTRY
The following clinical biochemistry parameters will be determined from serum
1 samples using the instruments listed:
Parameter '
Unit
Instrumentation
] Alanine aminotrans
pkat/1
ferase
1 Aspartate aminotrans (ikat/1 ferase
Bilirubin, total
pmol/1
Cholesterol, total
mmol/1
Triglycerides
l Creatinine
mmol/1 pmol/1
Glucose
mmol/1
Urea
mmol/1
Protein, total
fl/1
Protein, albumin
g/i
J Protein, globulin
g/l
Albumin Globulin ratio 1
Alkaline phosphatase pkat/1
: Sodium
mmol/1
Potassium
] Chloride
mmol/1 mmol/1
Calcium
mmol/1
Phosphorus
mmol/1
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Calculation {Protein total - Albumin] Calculation {A1bum1n/G1obul1n] Epos selective analyser 5060 (Eppendorf) EFIX 5055 Flame Photometer (Eppendorf) EFIX 5055 Flame Photometer (Eppendorf) Jenway PCLM3 EFIX 5055 Flame Photometer (Eppendorf) Epos selective analyser 5060 (Eppendorf)
- Page 12 -
000487
11/09 '98 18:29 FA* 31706418543
NOTO* B.V.
015/017
l_
______________________________________________ ___ ___________________________________ _
1
TELOMER ALCOHOL/TELOMER ACRYIATEI /PFHS/PFOS
NOTOX Project 242933
URINALYSIS (Recovery group animals only)
1 Urine samples will be collected overnight (approximately 16 hrs) from all animals assigned to the Recovery groups according to the following schedule:
- predose
1
- during the treatment period on days 7, 14 and at the end of treatment - at the end of the recovery period, prior to scheduled necropsy.
During the sampling period animals will be deprived of food but water will be provided. Urine will be collected Into a specimen vial, using a metabolism cage. After collection the samples will be frozen and stored at -20*C at NOTOX until further analysis.
PATHOLOGY
NECROPSY All animals surviving to the end of the observation period and all moribund animals will be deeply anaesthetised using ether vapour and subsequently exsanguinated. All animals will be necropsled and descriptions of all macroscopic abnormalities will be recorded. Prior to exsangulnation a blood sample (1*2 ml) for serum collection will be taken from the aorta. Serum samples will be stored at -20*C at NOTOX until further analysis.
J Samples of the following tissues and organs will be collected from all! animals at necropsy and fixed 1n neutral phosphate buffered 4% formaldehyde solution: Identification marks Adrenal glands Aorta Brain Caecum Colon
] Duodenum Epididymides Eyes with optic nerve and Harder1an gland Female mammary gland area Heart Ileum Jejunum Kidneys Liver Lung, infused with formalin Lvmph nodes - mandibular, mesenteric Oesophagus Ovaries Pancreas
- Page 13
000488
11/09 '98 18:30 FAX 31736418343
NOTOX B.V.
Ql 018/017
THIOMER ALCOHOl/TELOMER ACRYLATE/ /PFHS/PFOS
NOTOX Project 242933
Peyer's patches (jejunum, Ileum) 1f detectable Pituitary gland Prostate gland Rectum Salivary glands - mandibular Sciatic nerve Seminal vesicles Spinal cord -cervical, mldthoradc, lumbar Spleen Sternum with bone marrow Stomach Testes Thvmus Thyroid Including parathyroid. Trachea Urinary bladder Uterus. All gross lesions
ORGAN WEIGHTS
| The following organ weights (and terminal body weight) will be recorded from the | surviving animals on the scheduled day of necropsy:
Adrenal glands { Brain j Heart 1 Kidneys
Liver 1 Ovaries I Spleen J Testes
$
] Both absolute and relative (% body weight) organ weight data will be Included 1n 1 the study report.
."x
1 LIVER SAMPLING
* From all animals surviving until the scheduled necropsy at least 1 gram of the left lateral U v e r lobe will be frozen 1n 1sopentane/N2 . Liver samples will be
I stored at -20*C at NOTOX until further analysis.
I
i
- Page 14 -
000489
11/09 '98 18:30 FAX 31738418543
N0T0X B.V.
017/017
l--------------------------------- -- - _________________ ;
1
i TELOMER AlCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
NOTOX Project 242933
HISTOTECHNOIOGY
All organ and tissue samples, as defined under Hlstopathology (following), will be processed, embedded and cut at a thickness of 2-4 micrometers and stained with haematoxylin and eosln. Special stains will be used at the discretion of the pathologist.
I HISTOPATHOLOGY
v Slides of all underlined organs and tissues (see under Necropsy) collected at the scheduled sacrifice from all animals, as well as from all animals of all dose groups which died spontaneously or were terminated in extremis, and all gross lesions of all animals (all dose groups) will be examined by a
1 pathologist. All abnormalities will be described and Included 1n the report.
STATISTICAL ANALYSIS
The following statistical methods will be used to analyse the data:
If the variables can be assumed to follow a normal distribution, the Dunnetttest (many-to-one t-test) based on a pooled variance estimate will be applied for the comparison of the treated groups and the control groups for each sex.
The Steel-test (many-to-one rank test) will be applied instead of the Dunnetttest 1f the data can not be assumed to follow a normal distribution.
All tests will be two-sided and 1n all cases p < 0.05 will be accepted as the lowest level of significance.
Additional methods of statistical analysis may be used at the discretion of the Study Director. The methods and the results will be described in the report.
References:
I - C.W. Dunnett A Multiple Comparison Procedure for Comparing Several Treatments with a Control, J. Amer. Stat. Assoc. 50, 1096-1121 (1955).
i
J - R.G. Miller Simultaneous Statistical Inference, Springer Verlag, New York (1981).
fi 000490
- Page 15 -
TELOMER AlCOHOl/TELOMER ACRYLATE/ 7PFHS/PF0S
NOTOX Project 242933
DISTRIBUTION
This protocol will be distributed as follows:
Original: 1 Copy: 1 Copy: 1 Copy: 1 Copy:
1 Copy: 1 Copy:
Study Director Coordinating Bioteclmldan Clinical Pathology Necropsy/H1stotechn1que Hlstopathology Management Sponsor
.X
f
000491
STF HIS 325/03
H^NOTOX
Safety& EnvironmentalResearchB.V.
SPECIMEN DISPATCH LIST
NOTOX PROJECT: 242933______
SPECIES:
RAT
TEST SUBSTANCE:
83484, 83494, 83502, 84456, 84141
SIGNATURE/DATE STUDY DIRECTOR:
ShUrCQ-- -
1Q39
TO:
Name and adress company: 3 M Environmental Technology and Safety Services Attn. Kris Hansen 935 Bush Avenue
Building 2-3E-09
SsA ^ 'S f f S l ' j g l tel. 001 .6 s i T 32>5, CnnaruinT. Cca.se)_og
oov L s C P o - u - L Lexter)
55
Group 1 Group 2 Group 3 Group 4 Group 5 Group 6
Animal numbers
males
1-11 +
13-15
17-27 + 33-43 + 49-59 + 65-75 + 81-91 +
29-31 45-47
61-63
77-79
93-95
Animal numbers
females
97-107 + 113-123+ 129-139+ 145-155+ 161-171+ 177-187 +
109-111 125-127 141-143 157-159 173-175 189-191
Number of U v e r
samples
28
28 28 28 28 j. 28
Also enclosed are :
[ ] Copy of Protocol/Amendments [ ] Copies of histology raw data [ ] Study results [ ] Necropsy data transfer file
[]
DISPATCH: Signature:
Date:.
0 I7.i2f0>....
PLEASE SIGN AND RETURN THIS FORM AS SOON AS POSSIBLE AFTER RECEIPT:
COMPANY/SIGNATURE. C d & . A .
...?/*\ Date:... D ^ J . O? /. ^...
CC0492
^
Lisa A. Clemen
02/06/99 04:20 PM
Environmental Technology & Safety Seivlcea
(651)778-5568
3M Building 02-3E-09 PO Box 3331 SL Paul, MN 55133-3331 Fax: (651)778-6176
To: Kris J. Hansen/US-Corporate/3M/US@3M-Corporate Kail J . Rambo/US-Corporate/3M/US@3M-Corporats G rant A. Paul/US-Corporate/3M/US@3M-Coiporate
cc
Subject: NOTQX 242933 Sam ple Receipt
On 02/03/99 we received 168 rat livers from NOTOX 242933 (TOX120, W1220). These samples were received frozen on dry ice and have been stored in Freezer 9 at -20 +V10C.
Lisa
SAKPUE3 tJjEQjuffc 4 V B R i F i e b
000493
3H Environmental Technology and Safety Services
attn. Kris Hansen 935 Bush Avenue Building 2-3E-09 St. Paul, MN 55133-3331 USA
U M otox
SafetyftEnvlronmantalResearchB.V.
Hambakenwetering3 P.O. Box3476 5203OL *s-Hertogenbosch TheNetherlands Tel.: (073)6419575 Fax:(073)6418543
Date : 29-JAN-99 Ref. ; KIM
Subject: NOTOX Test Substance No. 83484, 83494, 83502, 84456, 84141
Dear Kris,
On request of Mrs. S. de Hoog herewith I send the liver samples (total 168) from NOTOX project 242933.
With kind regards, NOTOX B.V.
Mariette de Kinkeider Test Substance Officer
enclosure: a.m. Liver samples
000494
Chamberof Commerce VHertogenboschnr. 16055281
NOTOX Project #242933
P e b .4 ,1 9 9 9
The liver sample numbers listed below did not include a collection date on their containers. After reading the protocol, I wrote the collection date on each container. See below for dates. If these collection are not correct, please call me at 1-165*778*5368.
Regards, Lisa A. Clemen
Ac
Samples 1 2 3 4 17 18 19 20 33 34 35 36 49 50 51 52 65 66 67 68 81 82 83 84
Group 1 1 1 1 2 2 2 2 3 3 3 3 4 4 4 4 5 5 5 5 6 6 6 6
Collection Date Added 7 11/03/98 711/03/98 711/03/98 711/03/98 711/03/98 711/03/98 711/03/98 711/03/98 711/03/98 711/03/98 7 11/03/98 711/03/98 711/03/98 711/03/98 711/03/98 7 11/03/98 7 11/03/98 711/03/98 711/03/98 ? 11/03/98 711/03/98 711/03/98 711/03/98 711/03/98
Samples 109 110 111 125 126 127 141 142 143 157 158 159 173 174 175 189 190 191
Group 1 1 1 2 2 2 3 3 3 4 4
4 5 5 5 6 6 6
Collection Date Added 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 ? 12/04/98 7 12/04/98 ' 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98 7 12/04/98
000495
NOTOX #242933
TOX120 Liver Samples Received 02/03/99
Samples
-2 '3 -4 -5 *6 -7 '8 -9 '10 Nil >13 "14 <5 <7 -48 -19 "20 -21 "22 "23 "24 -25 . "26 <27 "29 "30 -31 "33 -34 "35 -3 6 *37 "38 -3 9 -4 0 Ml "42 '4 3 *.45 "46 "47 "49 -5 0 -51 -52 <53 N54 -55 "56 -57 *58 ^9 "61 -62 -63
Group 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 .3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4
Collection Date 11/03/98 11/03/98 11/03/98 11/03/98 11/04/98 11/04/98 11/04/98 11/04/98 11/18/98 11/18/98 11/18/98 12/02/98 12/02/98 12/02/98 11/03/98 11/03/98 11/03/98 11/03/98 11/04/98 11/04/98 11/04/98 11/04/98 11/18/98 11A8/98
11/18/98 12/02/98 12/02/98 12/02/98 11/03/98 11/03/98 11/03/98 11/03/98 11/04/98 11/04/98 11/04/98 11/04/98 11/18/98 11/18/98 11/18/98 12/02/98 12/02/98 12/02/98 11/03/98 11/03/98 11/03/98 11/03/98 11/04/98 11/04/98 11/04/98 11/04/98 11/18/98 11/18/98 11/18/98 12/02/98 12/02/98 12/02/98
Samples Group Collection Date
--65 5
11/03/98
>.66 5
11/03/98
"67 5
11/03/98
-68 5
11/03/98
"69 5
11/04/98
-70
5
11/04/98
"71 > 5
11/04/98
<72 5
11/04/98
"73 5
11/18/98
-74 5
11A8/98
*75 5
11/18/98
-77 5
12/02/98
*78 5 . 12/02/98
"79 5
12/02/98
'81 6
11/03/98
''82 . 6
11/03/98
-83 6
11/03/98
- 8 4 6 11/03/98
-85 6
11/04/98
<86 6
11/04/98
-87. 6
11/04/98
-88 6
11/04/98
"89 6
11/18/98
-90 6
11/18/98
-91 6
11/18/98
"93 6 -94 6
12/02/98 12/02/98
-95 6
12/02/98
-97 1
11/05/98
-98 1
11/05/98
-99 1 11/05/98
-100 1
11/05/98
J01 1
11/06/98
-102 1
11/06/98
"103 1
11/06/98
<04 1
11/06/98
-105
1
11/20/98
-J0 6
1
11/20/98
"107
1
11/20/98
"109 1
12/04/98
-110 1
12/04/98
-111 1
12/04/98
<113
2
11/05/98
<14 2
11/05/98
*415 2
11/05/98
<16 2
11/05/98
-117 2
11/06/98 '
<118
2 11/06/98
<19 2
11/06/98
"120 2
11/06/98
"121 2
11/20/98
-122
2
11/20/98
-123 2
11/20/98
-125 2
12/04/98
"126 2
12/04/98
-127
2
12/04/98
Samples \1 2 9 <130 -131
Group 3 3 3
Collection Date 11/05/98 11/05/98 11/05/98
<32 -133 -134
3 3 3
11/05/98 11/06/98 11/06/98
"135 3 <36 3
11/06/98 11/06/98
-137 3
11/20/98
"138 <39
3 3
11/20/98 11/20/98
<41 3
12/04/98
< 4 2 3 12/04/98
<43 3
12/04/98
-145
4
11/05/98
-146 4
11/05/98
-147 4
11/05/98
-148 4
11/05/98
< 4 9 4 11/06/98
< 50 4 11/06/98
"451
4
11/06/98
-152
4
11/06/98
"153
4
11/20/98
<54 4
11/20/98
\1 5 5
4
11/20/98
<57 4
12/04/98
<58 4
12/04/98
-159 4
12/04/98
<61 5
11/05/98
'-162
5
11/05/98
<63 5
11/05/98
-164 5
11/05/98
-165 5
11/06/98
"166
5
il/0 6 /9 8
-167 5
11/06/98
-168 5
11/06/98
-169 5
11/20/98
-170 5
11/20/98
<71 5
11/20/98
""173
5
12/04/98
<74 5
12/04/98
<75 5 12/04/98
-177 6
11/05/98
>178 6
11/05/98
-179
6
11/05/98
-180
6
11/05/98
-181 6
11/06/98
-182 6
11/06/98
-183 6
11/06/98
N184
6
11/06/98
-385
6
11/20/98
. "186 -187
6 6
11/20/98 11/20/98
<89 6
12/04/98
-190 6
12/04/98
<91 6
12/04/98
000496
000497
*%
; IX
/
iiraM^TTTyjff<B,Wnir5tfg
r ,
I. From (Collection Address)
Sendbig Depot Receiving Depot
::0 iSander'sAccount No. i |0|1|6|0|9|5| 2 1
` F'i w
NOTOX SAFETY & ENVIRONMENTAL
m *c RESEARCH BV
HAMBAKENWETERING 3
* -s.. ^ GD840494663WW
, a* 'S HERTOGENBOSCH iw/zipo*:
f Proving:
"**
Country:
5231 DD NL **''
-v'X .C on act Name f " . V J
X JL L
Tel No: 0 7 3 6 4 1 9 5 7 5
m 2. To (Receiver) (If a different delivery address is required please complete Section A)
U r i S M & kl& C
! < ? } If'.Addre 3 ^ r\0\<TVV\l<\fc,tXl "T C C-Uf) .&< jCaW M
f- e,ojiCflS, ( X t f ^
cuje\AM.e. J
i ovAA.tct-vvvn-"' 2l-"SE, ~ Oo Ccli
r 1* ; I
cWSt.ftwL*
Postal/ZIpTodc f W l O Y S l $ 3 - 2 l Country: ^
Contact Nam y f ( yf\ . U o . w s g . , v ^
T iN ^ c)o .i^ n a ^ (x 3 t8
A. Delivery Address (If different from Receiver's address)
y
/'M i m * '
CIV Proving: Contact Nam
Postal / Zip Cod Country: TeiNo:
?
3. Goods (If dutiable please complete Section B)
LGeneral DycrlpUon Anh NlM m fc P WNMnUtn k ri
No of . . .. PKkat w f *
Dtmenrieei (I
Check j m
B. Dutiable Shiment_Dotails (Comnlcir when .mnlignbloi
:i V ^o x l - u o t L U O u L
C \ L q fro a box b o ^
fi
: c W w a j! rO v .L
jO l * r C(Y v>V
- 6U m
te e .
1 y
JJ 't
XX
x . x ' 1
Xx
i
NaOFMCXMQir--- .................... -....... - - ^ . WEIGHT(LBS) 8AT
. 4,, HAA
' ' >
004150004
5 . 0 ^ ; Sat No.-"
4 . Services
* 0 :; _
Total'
OPS ftd u |* t Vfarifr.
VWfttt
\felmo
Vbfarrw WW^m
Sj
h
m
World Sendee*.
| | Kur t (t amMhwy) Q GawmtM i [2 j Priori
ORIGIN
LON
SHIPMENT NO.
M15Q0QM
4150004961
%1
004150004 ! 004150004
*-v'*r%' ;..''<j( *'' L|I JII'7PN-weittOettbArmytdfv.re^Wif
Invoke Q StfxMr'
f~ l * * .* >*tm ni . j
<Stn<fiiW*fcfwpfl^ry$mr*wctT*| |
%1 %1 %1
Nn
2*alfe.i 'VS^w,_/_:_
Y r -ji* -- ---------- -- ltu T O )N S SUMMARY SHEET
t
Study: Tir /<g> "/Analyst: ^ L/JZ./??
C
8ohcptftNNumb:/t&M'<SlS9 / Matrix: Y ^ 'p d c , ^
000498
Notts: /2T ^
/ / & " ur
/:L - 522=U^
+ ^^2/ y ff&z+t/s/p'
y
000499
ir-*?# -- -- --
Sludy-^Vie^
DafeMnalyst: JSt/tet?
ample num ber or dtsctpiHon
wr
A 3.
J__ _/3Sf?.
______
/3?r f
/ ^ T _ _____
dieutTons summary sheet
AtSJ& J/YpZ& r*
SobepOTN Number: /& b*' 7? w s& s?
Matrix:
Noies: /?*
"f s&>
%
t
I
000500
DILUTIONS SUMMARY SHEET
Study: T * * / t o
, , ,,, ,
Dilution Date/Analyst: s f a h q / A " **
Box#: '7% 3?&
Solvent/TN Number: f f ' * A / t ~V -A -J.J #
Extraction Date/Analyst:
M atrix/T im epoint:
Uve/'
. sample number
D IL U T IO N S
o r deserpition
1: ) r v 1: w o v 1:
1:
1:
1:
1:
1:
1:
Comments
too. l i f - a j r
X ___
(o ff-
M b-
lo s-p -
toe,F~
>o?f-
}
177F- & fttf-
1s ... ?C-
ft f-
Imp-
tV -F -
MPMP-
tfS F -
lUF,,
II?P-
n i9 /= 9fi=-
=^i
N otes:
I ' . f O P O '11 l u J
/
-
-- Ly
I'/ m J /-serf
t (OoOtJ r tfo ij-
1
*
DILUTIONS SUMMARY SHEET
Study: 'fb'Ar&fi Dilution Date/Analyst: QAc* i Box#: p-tiCfAtfi t
__ .W
Solvent/TN Number: MjU$ , 9 , Extraction Date/Analyst:
Matrix/Timepoint:y P d ^ tr
sam p le n u m b e r . o r deserpition
O SA 65
aA C lA
1:5*00
t *
I'.is o
.
v .a s
1:
1:
( j/ c< lA 7o A 7 /A
i
13/ 1 3 /A 7y /i
V,/ X
71/
j
n .A 77/ 17/ 17/ i7 /
v/ 63
/ X
%
n/ oiopP
'
a t/ 23/
\/
V/
Noles:^./ i ' S W - 3 'Q/jJ.
& ~ t f A c 'V l ^ L
f . z f O - V.O/J. S L -p lt : 796/ ^ l a Uj j H
I.as' A Vty*L
: 9 C,D/*l A c O H
DILUTION S 1: 1:
t
i
' *
I: 1:
. fAH,-XP, o o o J
Comments
000501
000502
DILUTIONS SUMMARY SHEET
Study:~fo%ft2Q
_
Dilution Date/Analyst: tC IIWJAP
Box#: W-'VClftiG
'
Solvent/TNNumber:Midi, JhrA'&OT Extraction Date/Analyst: /5Aprfffl, fM XPt J'fir*
Matrix/Timepoint:AdUyjcT
sam p le n u m b e r
DILUTION S
o r descrpition
1: 2 S
1: / 0
1:
I:
I:
1:
1:
I:
I:
Comments
' -& /A
X
uH /
(
- Z G aA ________ /
- 3 3 A ____6 3 ___________ 3V /I
3 iW
3L/ 3 1 /A
3M
1 7 /1
HO/ ^/
%//
y j A c,2 2 9 /1
3O A
n /1 J
X A
m m
<42/1 6 3
45/ w
1
1
H C / J/
V/
N otes: A f : Q f = H O ^ l f ^ k
Mi
l:fO - fOO^L f*yfk : JOQ/-L / O 0 v
*
. ..
*
DILUTIONS SUMMARY SHEET
Study: -fbX-lQ.Q
Dilution Date/Analyst: B ox#: 19-0(,IAtQ
f7^ / 3 ^
Solvent/TN Number: /MK, -rU -A -W f
Extraction Datc/Analyst: Apr UH,dtPt -pp
Matrix/Timepoint: at Lvcr
'
sam p le n u m b er
D IL U T IO N S
o r descrpition \ u o I: 1: I: I: 1: 1: I: I: Comments
itfM 6 3
\
t
000503
.
,
* .
/
!
*
Notes:: dL>( tto - W O ^ L r^yik, 1M/*L ft)
%
Study:Tox-iao Dilution Date/Analyst:
* *' 9 9 - 0 (a A f>
sam ple number or dtserpition
4M >3
HO b io
DILUTIONS SUMMARY SHEET
^ Solvent/TN Number: MxH (T ru -ri -q s o (* Krtr^dk Drt^Aiudyst: W f-f^//z ji/w-jc/>-s u r - w y
MWrhmmepoliit:p .^ u ^
DILUTIONS 0 1: 1: 1: 1: 1: 1: Comments
S '3 7- ? ? S c*-'
------ -
000504
*
.
'
. /
0
*0 o
^ ------------------------
Notes:
(Moto
L ffi SOJrtfi*
^OO juL
n u o ti
*
0
DILUTIONS SUMMARY SHEET
StadrTbX-120 .
DihiUonDale/Analyst:"/<3/99".AH
SExotlvreanctt/iToNnDNautme/bAenra.Mlys(t6:W)/i fJ
^ *-< r)- b n ____________________ __________________ Nfatrlimmepotoh
/|VfK /
ordescrpitionsa m p le n u m b e r
D IL U T IO N S
1 0 0 0 I: 1: I: 1: I: I: 1: I:
% l M t Gvlff
D lO O fl
j TIM . . H M . TKM S
1
Tl.M .
nM . M ,
.
M ,
Comments
>IM . KM ,
Ihm,
K .m ' L_______
\L _
.. r b r l T 5
c>
* "4 m
0
Notes:
DJOOO -
ScmpU f IOOOmL wdbaml
-------------v -
-
--
,
/TCP
000S05
000506
DILUTIONS SUMMARY SHEET
Study: iW rt^O Dilution Date/Analyst: PTM* g>0X 99-W A*t>
Solvent/TN Number:
^ ^ W SS
.
Extraction Date/Analyst: y //? /? ? j IVSi/jCfi/SCr/SAf/
Matrix/TImepoint: f r f ^ / f r 0Ur f ____________
ordescrpitionsam ple n u m b er
DILUTION S
I: 7 0
1:
1:
1:
1:
1:
1:
1:
1: Comments
WM 6H
0/4
SOM W
1
StM M
O M iV SiM S-tf SUM M
-
mw
V/
*
:
`
/ % %
* Notes:
Oio- oomL sw p U t I mL /m ^ W
%
v
Sample weights/Volumes
Prep Date(s): Analyses): f l 3 C )9-~ Sample Matrix: Pli r L-i u * * Method/Revision: /-'/T c 7"'/'! -f> O
Target Analyte(s):
/
Study Number *T~0 K ( P - 0 Equipment Number: Notebook# and Page: Final Solvent/TN Number
1MG1 2MG1 3MG1 4M Gl
] 3MG1 6MG1 7MG1
1 8MG1 9MG1 OMGI UM Gl 13M Gl 14M Gl 15MG1 17MG2 18MG2 19MG2 20MG2 21MG2 22MG2
J 23MG2 24MG2 25MG2 26MG2 27M G2
Sampie ID
Initial Wt/VoL g/mL/L
,6 s i ?r /0,/g f ? 9 >,5/2~
A.&91L0 /9*. A). 9a.S
0. 3W
#ml MQ Water Added
33 3 . S"
`C> 3M V-S
/. 1 ... 3.2, 2.S
V-3
/ . 7 / SM
M /W 4 M S //
H.S V.3
A 503
S3
> -9 9 ? 3
S.o
33
^5^3'/
2 .1
Pf (2, h S 7 ? - ' 9 .0
A 3 & F ir 0 72VT 03910
S3
3.6 3.0
o stri
ti7 3 * i
0.9/7^
7. 7 9iC
Density(g/ml)
.\
Comments
' ^--
>
*
*"! .
J /^(i
Alf
J ffL fJ
flrl1A i e j
SU
fjr
by O h
( M i *~p
}*f h /*,/&
/ USf
u
O /? 9 V *tf>l)r J jty f lg }
/
Notes:
> a m K-y
^
s n /s r A ?
J
Extraction-1 Template Review Date______ _
Form Approved by/Date:_
\
Weight Volume Sheetl20.xls
000507
Page 1of_
Prep Date(s): Analyst(s): A jJltbJcP 'J'A P Sample Matrix: Method/Revision: fA -fT -'/l-ftQ 1 Target Anaiyte(s):
Sample ID
29MG2 30MG2 31MG2 33MG3
] 34MG3 35M G3 36M G3 37MG3 38MG3 39MG3
40MG3 41M G3 42MG3 43M G3 45MG3 46MG3 47MG3 49MG4 50MG4 51MG4
J 52MG4 53MG4 54MG4 55MG4 56MG4 Summary of method:
]
Sample weights/Volumes
Study Numben / O Y f j u p Equipment Numben Notebook# and Page: Final Solvent/TN Numben
Initial Wt/VoL g/mUL
#ml MQ Water Added
Density(g/ml)
45/3 -Pfr / /
/>*SS S W
.<&/*/ H * .V& 3 Z -
O.GHS- 3.H
.ra sa Sb/3. O e>-
/ . w a s 4.>
3 .0
as?-`/_ LA
.iSbio Z .L 3/3~ a )
/. /53 J,73/3 3 .7
V.3T
s: 3 ~y&
~
M ?//
3, i
3., A.V/5- 3 . 2
.sSit Z .S
Z.l YO
25%? in q >.5-/9 3 -3
..
Comments
Notes:
<D Z
!
1 Extraction-1Template Review Date__________
Form Approved by/Date:.
WeightVolume Sheetl20.xls
000508
Page 1of
PrepDate(s):
] Analyses) : / # j A P
Sample Matrix:
Method/Revision:
D
Target Analyte(s):
Sample ID
57MG4 58MG4 59MG4 61MG4 62MG4 63MG4 65MGJ 66MGS 67MG5 68MG5 69MG5 70MG5 71MG5 72MG5 73MG5 74MG5 75MG5 77MG5 78MG5 79M G5 81MG6 82MG6 83MG6 84MG6 83MG6 Summary of method:
]
j Notes:
1
I
Sam ple weights/Vohimes
Study Number: *TPX / Q
Equipment Number Notebook# and Page: Final Solvent/TN Number:
Initial WL/Vol. g/mL/L n .m i d . q a i _
/. m i l ? t.o i
/.a m o.xo& t d.1Y73 t.O & Y O .Q T 7 7 0 .1 9 o 3. &95/C, t.o u rc 1.027
0.99 o 0.9*130 o .ifo ? 1 .0 9 0 3 D .qw j 0 .-7 1 6 V
0 .9 7 0 3 0 .1 7 a S ' /.o il!
#mlMQ Water Added
.T H.C
?.v -
ny f.y t/. x 2. / 0
--
sU y .9
</.Y -r.3
! 3 . 3 f.O
to
3. Jr
y .i 1 .7
3 .9 **3
Demity(g/ml) Comments
, ..
Form Approved by/Date:.
Extraction-1 Template Review Date________
Weight Volume Sheetl20.xls
000509
Page 1 of
Prep Date(s): y A f f o 9 Analyses): Sample Matrix: / t x f / v y * Method/Revision: p ^ - r p - r f - f . Z> Target Analyte(s):
Sample ID
86M G6 87MG6 88MG6 89MG6
] 90MG6 91MG6 93MG6
1 94MG6 95MG6
Sample w e i g h t s olumes
Study Number: "~p Equipment Number: Notebook# and Page: Final Solvent/TN Number
Initial Wt/Vol.
(dmUL
C>'9Pt .Z37X M l Li 1>%sO
i-o n L I.T33 , 9 ItOJ-Y
1-/037
ftnIMQ Water Added
y* yz `t ' 9 s .9 SI3 S* J&v/ Y* S S7f S* S
Density(g/ml)
Commenta
-
A
J *t
3
Notes:
i
!
J Extraction-1 Template Review Date________
Form Approved by/Date:
Weight Volume Sheetl20.xls
000510
Page 1of.
Extraction Worksheet ETS-8-4.0
Study # T / ? y >1.0
Surrogate Std
Box #. 9 f ^ C 7 A / 6 MatrixJ U jU * J L -
approx. ppm actual/#) ppm
#
AL *UQ 9 __ -A.6Kiit-H.l- __ _________
-LAZ/U-tL
-SaJ - N I -
-/Ajt.k--N )-
FC -M ix
approx. 0.5 ppm
actual
ppm
______
FC -M ix approx. 5 ppm actual ppm # 99t>// - / - )
-
FC -M ix approx. 50 ppm actual ppm
* 9 9 * //Y J l<J -
Date and Initials for
Std. or Comments fitti M-W*<
ta.lM J 7 ^ .0 7 1
1 l1^ *L-M^
\M Cl pM Cl 0 Cl m Cf KM Cl
Vt
io J
- -
10J.
7D
-
- .-
I/
JCP-Si i
LM ir! m rr fif Cl ?M iM IbM C l UM C l IM C l m Cl km a FfM C 2 m 6-1
--1
Vr
--
-
.-
-
- -
, ^ * '
t
1Study num ber w here tihe originiil workshetf is located. ____;_______________:__ ____,-_ _ i-- L_-- , " ~
Blank / / , U J / . ^ a
Std# - ? 01 1 - S^2t>
am ount-
ItO
mL fa . 1 7 9 4 a L /S
Serum Extraction Method
:
P <
Date & Initials-
Vortex 15 sec. Pioette Matrix
Volume
A
ml
Y/re/fQ `
Pipette 1ml of 0.5 M T B A pH 10.
Std.# 9 9 H - Y - 2 .
S A U l I Z I jUIjJ V / l if o ?
Pipette 2 ml o f025 Na?COV0.25M NaHCCh buffer Std. # 9 9 a / U SS~A '
Dispense 5 ml of methyl-t-butyl ether
TN-A- 2 X
Shake 20 min. Centrifuge 20-25 min.
Centrifuge speed: .A T ^i>
Remove a 4 mL aliauot of oreanic laver
Put on Nitrogen Evaporator to drvness Evaporator#:___________________Temperature:__________________ __
Add methanol
Volume 1 .0
ml
TN-A- aM Zfc
Vortex 30 sec.
Filter using a 3cc B-D svrinee with a 0.2um filter into a 1.5 ml autosamole vial
>
n o n v i o v t / p v*uiu. u i c t iu . o p iN c u t u uju u i a
__ ' i_r_ / i ppm. MS/MSD used ssaample )
Cont. Checks used same m atrix as for std curve.
SJuurrrroogate Standard: Spik'--ed1 J~ uL o f a S~~ ppm std f 9 9 a / / ) to all sam ples, standards, and blanks
(j) fth-nxp I-
<. J r t 6 ) } MS2.-t-tjpi.~j/nllc
/JStf
Attachment A
ETS-8-4.0 Extraction of PFOS from Serum
Page 10 o f 13
000511
Extraction Worksheet ETS-8-4.0
W OE !BOE Jit>TBF`lCW Surrogate Std
B o x m - g u t M t approx. ppm
Matrix J2s /VA r
actualJOO PPm
FC-Mix approx. 0.5 ppm actual ppm #
AA f r i a m fri -
____C m L______
.-
1 M fri aaAf f r i -
3lM fr i -
MM fri-
S tM f r i -
aiA t fr i ~
aw # fr i-
fo/M f r i
n m fri
m fri
U M fri ViM ( t i
.
S to iti
VM ( t i
9 A fri
3tM fr i
fri W M 64 VM fr i
* ilM f r i ____________
-
1
Si
TAI f r i iti
V?M fri M P i
sm M
.
%A ________
-
ASudx .number where thie original workshSSit is located.
Std# s i f w a m .
Serum Extraction Method
FC-Mix approx. 5 ppm actual ppm #
-
FC-Mix approx. 50 ppm actual ppm
It -
-
Date and Initials for
Std. or Comments
1 1
1
-
_ . ,, amount ]_. O
1---------T - ____ i /
mL^inQ^ e J k h Sii,1
Pate & Initials
Vortex 15 sec. Pipette Matrix Pipette 1 ml o f 0.5 M TBA, pH 10.
Volume Std.#
Ac?-
ml
?/(*/**s i O *. ~tr
& mzmswmm
Pipette 2 ml of 0.25 Na2CCh/0.2SM NaHCCh buffer Std. # 9 9 o /P -fT ^
Dispense 5 ml of mcthyl-t-butyl ether
Shake 20 min. Centrifuge 20-25 min.
Centrifuge speed: 3Sl)f) Spi*1
Remove a 4 mL aliquot of organic layer
Put on Nitrogen Evaporator to drvncss Evaporator #:
Temperature
Add methanol
Volume A O
ml
TN -A -SH M .& > t f j
Vortex 30 sec.
Filter using a 3cc B-D syringe with a 0.2um filter into a 1.5 ml autosample vial
________________________
MS/MSD/ I f Cont. Checks: Spiked 10 J tL o f a
ppm std (
'l for a final concentration o f
C )r 3 0 ppm. MS/MSD used sampled?) UgI jTUJ ___ . Cont. Checks used same matrix as for std curve.
Surrogate Standard: Spiked ( 0 uL o f a C ppm std (
) to all samples, standards, and blanks
3M6-I f MSaM spS - spitt itMi-l, M
-piti 13*6-1
A ttachm ent A
E T S -8 -4 .0 Extraction o f PFOS from Serum
P a g e 10 o f 13
000S12
Extraction Worksheet ETS-8-4.0
>
i
Study Box W M M B Matrix E r t f i W
ST//M (rH SA V -
obrj/M* ? cSM
b#
(Ur fi -
AV-
6-V -
~~
Surrogate Std approx . ppm actual/ ppm # 9 fo /J
I FC-Mix
approx. 0.5 ppm
actual
ppm
#
-
-
FC -M ix approx. 5 ppm actual ppm #
-
FC -M ix
approx. 50 ppm
actual #
ppm
-
-
Date and Initials for
Std. or Comments
'
STA1 A f -
4/H A V -
1
/A M
--
& M &*/
-
M M tr*i
-
fcCAf A.C U M AT
-
-
M M fri:
-
MM AC
-
MM a t
\ \P
WlAI A T AT
-
^A 4 AT JIM AC
-
AT toW A C
AC
-
-
-
?fA f AC
--
--
IF 7 K 7 K 7 9 H H H
% L_______
-
-
- t~
1Study num ber w here thie original w orksheet is located.----------------------------------------------------------------------------------:--
std# w a - c * a o
amount = !,(j
1Serum Extraction Method
Date & Initials
Vortex 15 sec. Pioette Matrix
- P.-- ~ ' Volume /. t? ml ------------- s:--- H Z
Pipette 1 ml of 0.5 M TBA, pH 10.
Std.#
Pipette 2 ml o f 0.25 NaiCOV0.25M NaHCCh buffer Std. #
_ /s~o
w y** ^
Dispense 5 ml o f methyl-t-butyl ether
TN-A- 2 JT<9
Shake 20 min. Centrifuee 20-25 min.
Centrifuee sneed: 'idX)/tY\
/
Remove a 4 mL aliauot of oreanic laver
Put on Nitroeen Evaoorator to drvness Evaporator #:
Add methanol
Volume l*0
ml
Temperature:
TN-A- ZV3K
1Vortex 30 sec.
1.
Filter usine a 3cc B-D svrinee with a 0.2um filter into a 1.5 ml autosamnle vial uL i
_________________
0 . 3 r d ppm. MS/MSD used s a m p l e _____________ Cont. Checks used same matrix as for std curve.
Surrogate Standard: Spiked 1IU0 UuLL otf a
ppm std ( 7 7 0 / / - // t o - / )) to all samples, standards, anud boliankxs
<DMSjmpl-Sf'ilt 3M 6~lj AASa/MS0-5f))ce (jM fjt M3/MSP3 'S f i &
A ttachm ent A
E T S -8 -4 .0 Extraction o f PFOS from Serum
000513
Page 10 o f 13
Extraction Worksheet ETS-8-4.0
Study U T X -t Box A4-8 Matrix E A f /V<Y
Surrogate Std approx, ppm actual/O ppm
FC-Mix approx. 0.5 ppm actual ppm
#
FC-Mix approx. 5 ppm actual ppm
FC-Mix approx. 50 ppm actual ppm
#
Date and initials for
Std. or Comments
iy/o -fe.-ftrft fr yfl_
n m &<r
INLJrk.z.
66-
6L J *.
'}M frit -
Of
$ H M 6r(r
fm J i k .
J f l 1 - rfiP,
o nnT~i!
r
M&1 rH75
/ J M M -- MS'
TEL
t JL
MM (rio
M l : or swvu / ^ g - L h j r f or/l, S A t t X 7l*>/79 -
a
h/f
-
AS
V
s
1study number where the original worksheet 5 tocatL
Blank K flM irf F ivelT
Std# m j p - W z Z T T
amount8
Serum Extraction Method
,/ajr Zzfr..
Vortex 15 sec.
Pipette Matrix
Volume E E
ml
Pipcttc 1 ml of 0.5 M TBA, pH 10.
Std.#
*/~2-
jU L
mL m n x i t i m t j k i t Date & Initials
Pipette 2 ml of 0.25 Na?COV0.25M NaHCOt buffer Std. # h e / / --/ ? O
Dispense 5 ml ofmcthyl-t-butyl ether Shake 20 min. Centrifuge 20-25 min.
TN-A- jZ ? S Centrifuge speed: Z*Ul'ff
Remove a 4 mL aliquot of organic layer
Put on Nitrogen Evaporator to dryness Evaporator #:
Add methanol
Volume I*0
ml
Temperature: TN-A-
Vortex 30 sec.
Filter using a 3cc B-D syringe with a 0.2um filter into a 1.5 ml autosample vial MS/MSD/ IK C ont Checks: Spiked 10 uL o f a S* ppm std (
^/ ) for a final concentration o f
_ ppm. MS/MSD used sampled?) h , f j /~Ia J
Cont. Checks used same matrix as for std curve.
Surrogate Standard: Spiked 1 0 uL o f a A ppm std ( % / / - / - , / ) to all samples, standards, and blanks
M Sl/M S0/-spi& 3/l4fy rftejAASOJ-sfijce bM MS3/MSPJ - y l f a !3M l
Attachment A
ETS-8-4.0
Extraction o f PFOS from Serum
000514
Page 10 o f 13
IonPair StandardCurves--TOX-120(Rat Liver)
PrepDate(s): Analyses): SampleMatrix: Metbod/Revblon: TargetAnalyte(s):
4/19m
SAH/RWW/JCP/SRP
RatLiver
FACT-M-1.0
POAA, PFOS,
,PFHS, M513andM613
FC Mix StdApprox.0300ppm: FCMix StdApprox.5.00ppm: FCMix StdApprox.50.0ppm: SurrogateStdApprox. 100ppm:
99011-16-2 99011-16-1 99011-124 99012-3-24
. ActualConcentrationsofStandardsintheFC Mix
POAA
PFOS
StdCone. ug/mL
StdCone. ug/mL
StdCone. ug/mL
0.500
0.500
0300
0300
0300
0.500
0.500
0.500
0.500
0.500
0300
0.500
0.500
0300
0300
5.00
5.00
5.00
5.00
5.00
5.00
5.00
5.00
5.00
50.0
50.0
50.0
PFHS StdCone.
ug/mL
0.500 0300 0.500 0.500 0.500 5.00 5.00 5.00 50.0
CalculatedConcentrations ofStandards in theSampleMatrix.
POAA
PFOS
Final Cone.
Final Coric.
Final Cone.
ng/g
ng/g
ng/g
5.56 11.1
536 11.1
536 11.1
27.8
27.8
27.8
55.6
55.6
55.6
111 111 111
278 278 i 278
556
556
556
833 1111
833 1111
833 1111
PFHS Final Cone.
ng/g
5.56 11.1 27.8 55.6
111 278 556 833 1111
StudyNumber TOX-120 EquipmentNumber Final SolventSt TNNumber MeOH/TN-A-2438 BlankTlssue/Identifler RabbitLiver/ 99012-5-20
M513 StdCone.
ug/mL
0.500 0.500 0.500 0300 0.500 5.00 5.00 5.00 50.0
M613 StdCone.
ug/mL
0.500 0300 0300 0300 0300 5.00 5.00 5.00 50.0
All AmtSpiked
mL
0.002 0.004 0.010 0.020 0.040 0.010 0.020 0.030 0.004
All Density
g 0.180 0.180 0.180 0.180 0.180 0.180 0.180 0.180 0.180
MS13 Final Cone.
ng/g 5.56 11.1 27.8 55.6
111 278 . 556 833 1111
M613 Final Cone.
ng/g 536 11.1 27.8 55.6
111 278 556 833 1111
Surrogate StdCone.
ug/mL
100 ' Surrogate Final Cone.
ng/g
2778
All Amt Spiked
mL
0.005 Surrogate Final Cone.
ng/mL
500
POAAJFOS,. ,PFHS.M513J4613.xls
000515
/>?X 7?~37A*t
Ion Pair Extraction Worksheet
Study # T o*
H-,0 Blank - { 4 -- /h iis t- t v e r Blank-/?. W L0 A
1 _______ - t n J -
______ - S S - 1 -7^ -
Surrogate Std approx. ppm actual M t^ p p m #W ^ A tH U tf
FC M ix Std
approx. 0.5 ppm
' actual
ppm
#W 990//V S -L
JsJ 0 * 4 _______ i_
FC M ix Std approx. 5 ppm actual ppm #W 990//-U -I
*
-
10J
FC M ix Std
approx. 50 ppm
actual
ppm
#w 9 ? o //-/i< l
Date and Initials for
Std. or Comments
w n a zn m \
-- V " m"f m
'
I ~ 7SHa*k ~ 1______ - / t t e L i s l >
/ ~ 2s i.'i-sst.H t r
f)f4. le s .
M5
f)NO. llO p---l-lSdnA , HIS tL> I M w
s'
-
.3 e J
fod
7D.
/s.
/ ( /ttiu ! . r f t '. o e S
s~ -J~i *-i ts f r u /! >
_____^
Shf
>*
_ -- 1--
___ - --
----- ' -- -
___ -- ^
!
i
= = = = = ----------- =
" -------
Blank B A I ,+L,u* r
Std# ^ X 0 ? 9 -/S ^ 3
amount =
Extraction Method Number/Revision:__________________________________]____________________;_____________
Date A Initial
Add Surrogate, Vortex 15 see__________________________________________________________________ S JU + IK U M
Pipette Sample
Volume
A t? mL
Pipette 1 mL o f 0.5 M TBA, pH 10.
Std. # G ? o 9 ^ S * r - /J
Pipette 2 mL of 0.25 NaoCOV0.25MNaHCOt buffer Std. # 7 9 f ? -<?
Pipette 5 mL of-ethyTacetate
fa tJ - AhiJt4 TN-A- X Z S 7
Shake 20 min. Centrifuge 20-25 min.
/ Centrifuge speed: kJ 'O oc>
Remove a 4 mL aiiauot o f organic laver
Put on Nitrogen Evaporator to drvness Evaporator #:
Add methanol
Volume / , c) mL
Temperature; / ( . SS-bJA_____________________ TN-A- 3?3Z S '
Vortex 30 sec.
1Filter using a 3cc B-D svrinae with a 0.2um SRI filter into a 1.5 mL autosample v i a } ______________________________ i t ____ --
M S/M SD/ f y ConL Checks: Spiked (0 u L o f a S ~ o o ^ std i f9 eJ )-S S -t ) for a final concentration o f
3J1? opl . M S/M SD used sample M o ^ r
. Cont Checks used same matrix as for std curve.
Additional Comments:
000516
f r y 7-43=tA*B
r L _______ _______________ .
IonPair StandardCurves-TOX-120 (RatLiver)
Prep Date(s): Analyst(s): SampleMatrix: Method/Revision: Target Ana!yie(j);
2/22/99 RWW/SAH RatLiver FACT-M-1.0 POAA, PFOS,
PFHS.M513&M613
StudyNumber: TOX-120 Equipment Number: Final Solvent& TNNumber MeOH/TN-A-232S BlankTbaue/Identilier RabbitLiver/98099-15-3
FC MixStdApprox.O.SOOppm: FC MixStdApprox. 5.00ppm: FC MixStdApprox. 50.0ppm: SurrogateStdApprox. 16.5ppm:
99011-16-2 99011-16-1 99011-124 98094-12-24
1 ] 1
I
1I
Actual ConcentrationofStandardsintheFC Mix.
POAA
PFOS
'
StdCone. ug/mL
StdCone. ug/mL
StdCone. ug/mL
0.500
0.500
0500 .
0.500
0.500
0.500
0.500
0.500
0500
0500
0500
0500
0.500
0500
0.500
5.00
5.00
5.00
5.00
5.00
5.00
5.00 50.0
5.00 50.0
5.00 50.0
PFHS StdCone.
ug/mL
0500 0500 0.500 0.500 0500 5.00 5.00 5.00 50.0
M513 StdCone.
ug/mL
0.500 0.500 0.500 0500 0.500 5.00 5.00 5.00 50.0
M613 StdCone.
ug/mL
0.500 0.500 0.500 0500 0.500 5.00 5.00 5.00 50.0
CalculatedConcentrationsofStandardsin theSampleMatrix.
POAA
PFOS
Final Cone.
Final.Conc.
Final Cone.
ng/g
ng/g
ng/g
5.99
5.99
5.99
12.0
12.0
12.0
29.9
29.9
29.9
59.9
59.9
59.9
120 120 120
299
299 299
599
599 599
898 1198
898 1198
898 1198
PFHS Final Cone.
ng/g 5.99 12.0 29.9 59.9 120 299 599 898 1198
M513 Final Cone.
ng/g 5.99 12.0 29.9 59.9 120 299 599 898 1198
M613 Final Cone.
ng/g 5.99 12.0 29.9 59.9 120 299 599 898 1198
J
1 ] 106F-G1
110F-G1
ms/msd 106F-G1 I10F-G1
POAA Final Cone.
ng/g 299 299
InitialWt. (g): 0.9240 0.9242
PFOS Final Cone. .
ng/g 299 299
Final Cone. ng/g 299 299
Initial VolumefmLV 4.6 4.6
PFHS Final Cone.
ng/g 299 299
M513 Final Cone.
ng/g 299 299
M613x Final Cone.
ng/g 299 299
AU Amt Spiked
mL
0.002 0.004 0.010 0.020 0.040 0.010 0.020 0.030 0.004
AU Density
g 0.167 0.167 0.167 0.167 0.167 0.167 0.167 0.167 0.167
Surrogate StdCone.
ug/mL
16.5 Surrogate Final Cone.
ng/g 494
AU Amt Spiked
mL
0.005
AmtSpikedfmLV 0.010 0.010
.J
POAA.PFOS, .PFHS.M513.M613
000517
Sam ple W eights/ Volumes
Prep Date(s):
1 Analyses): f a o I Sample Matrix: flifi-t
1 Method/Revislon:
Target Analyt*(s):
1,0
Study Number. Equipment Number Notebook# and Page: Final Solvent: SfA
f o
. f
Sample ID
io.97P-D/ />m. 91
*
0 / / 0. /00 jP-- &-) r)N.a! t?~t f) Di. )oJ F--D}
Initial <^yvoL
/fonUL 0,nz 0 a </JJJ
0, J( .
0, w V 0, C.S70 ...
. M 2
o,rifc.
r 9X*/D
DH. to? D-6-!
>, </JJ~9
O//0> (>*7f 6~{
' `/ / Z D
Ain. h f - M
aM . lIF- 6-1
6.92V2
0 .9H29
/Vim . \rtF-frli
. ZO
ntia. IMF-6-3 DA/i UPF- 6-3.
h.W 9r b,0709
t.UOF-6-l
ht 7-33ST
DrJti, HI F`6-3 fan. l/t F-6-1
. 0.C3V .902
P/\Ja. 11$F-6-2
.P'm
OaJsi. /OF -6-3
,H29r
Offa. 131F- 6-3
6.937-2
Cai*. 133 F -i-i_______________________ h. 039/
cU h & O j
Comments
l/hl ihr g m / m t
a.(. 0.11,7 P . H - 6./OS' i . s ... 6 .1 7 2
3 .0 -
..St.^_____
p ?< r
y-i. -
?s Htir
j Q u l O O __________
0.100. _
./OH Ci.(00 h.ior n./Oc a.io?
2.0.
./aT
.l
/.O
./00__________
0.11,7
Ht % JO
A 6 . M
3. Z O - M 3.9 '0.100
3.0 ./0?
3 .3 .ior
P U ./fer
2,1 0.100 PU 0.170
9.2 h, 100 3./ .M
] Summary of Method:
Notes:
J
1 Extraction-1Template 1 Review Date______
Fona Approved by/Date:. Weight Volume Sheebxls
000518
Page 1of
Sam ple W eights/ Volum es
Prep Date(s): P - ( i j ^
Analyst: f c M (S A H Sample M a t r i x : ^ / -
Method/Revislon: pAfTZflA -J,
Target Analyte(s):
Study Number; YO )(*" / 2 Equipment Number: Notebook# and Page; F inalS olvent:^*///. ^ o M r
Sample ID
Initial ztSnpIVVoLL
mL Meier
Mn. MF-e-a
.M
aIa. i?rF-6-X
o.su?v V0-
' Al.
. W 3 .9
i Alp./ai-F-
. 3t,0_
3. /
' 0Ak./3F-<r
.3CI2 -
ONn. M F - <j
O.'ttZ
oma.
.'rff
m*. lilt-6-3
0.32/! _ A ?
JDa}A.IFP- &3
a 0/3/ .. ?,/
,Mfl. /ITT-3
o.rs/,9
?
Dftlfi.l2UF~6-3
2.9
J 0AlA.il??-i
. m n V.
jDAi/i.l2F~6r3
.(,*3$r _ 2J
Aifi.ttfF-frl OaJ/. W P ^ I
..c o f o . 2.2 .003 3.3
] PAJn.mF-Fi
o,?*r? V,0 _
J 0A)n.W?f-6*
3* 7
DM, &.JH1 /,?
jOn /).Ht-6-4
JH/t3 5./
J N. iw/e-frJ
O,WS*?
, f)N/>. HX/T-fr</
H/,99 5.3
j D/S. tWf-r
,&0?7 3,0
J f)rfO`iSoF-Ty_ _ _ _ _ _ _ _ rOVtC - 1 x 2 --
junoty Comments
3/#>C
OJle? O.llp? .&?
___ o-iWt_ _ _
0-n.f0-I/.SJU
r>Jb?_ _ _
JS" DM O.ti? 0.1(*2
to'ib'i__
n.H.'* o.li.9 .Mrb O/bb OJFY 6.M fiJUt 6.m h.m (Ut. ?
J Summary of Method:
Notes:
J
,
Extraction-1Template Review Date______
Form Approvedby/Date:. Weight Volume Sheetxls
000S19
Page 1of
Sam ple W eights/ Volum es
Prep D ate! z / t i f a
Study Number: / / - / 2 - A Equipment Number
Sample M a tite Method/Rerlsioa:
/ 6
Notebook# and Page: Final Solvent
/ ,/
Target naiyte(s):
SaImDple
$VIni/tViaolL dbM'ty Comments
AfmLJL k/aU r
W lrtL
nrfo frfA - -tf
- 51-P--AH n^,o. /r?/? -s</ n/ta,/JV /Z-9
A. ryy 'C t
.Z s? $ >, 72.2. f t'Z o l
2 .7
3.2
9. 3,0 9.0
_CU(,1
./r .M/&
__ J 1 * 2 _n./6>?
ntj. t r z P - o Y hrta. r s v f - a 9
o.ZfF l
Z ,rys0 _r<n?<~
9.
79 ,0
_6 M
' nfo? o -m
//\f)M, U / f - a -r
f)7/ 1
A ,fX f9 0 . * j9
9.9 3 .9
o -n . z 0 ,/u i
f)NA. / /
A-3~
6 ,1 /0 ?
9J
o ./i^ r
/)///>. { s '/ '' o s '
6,1/779 `*?it9
3.?2 .9 6.l(pb o .iu r
o ,y irz
2.1
DH0. (6 7F - O S '
.< m z
2 .9
rOHt ((.7/C~-S'
(7fi/ f - '/ S r
f Tj<H
32 2.7-
./t-
. D M , !7a F - A S '
. ??r? 3 1
.Kfi
f7f F - A S '
/?Ha>.
S'
J[)/Ja , n i r - ~ A r
,r c ? i '7 7 t 6,1937
2?
9.9 9.0
./M 0. Msb .lbfo
Dno,
A<r
p,</z,z
2.5
n ./i T
\ t/. n i/P - t- r .
___ _^0f9. f 7 f JSummary ofMethod:
6,7 TTZ 3 1 'YYZ-2-,. - A ,2
_, .M
MT-
1.
Notes:
Extraction-1 Template Review Date______ I
Fono Approvedby/Date:. Weight Volume Sheetxls
000520
Page 1of.
Sam ple W eights/ Volumes
Prep Date(s): Analyses): / l n / d / T A t f Sample Matrix: A tf-i-t* Method/Rerision: f A - r T - r t " / Target nalyte(3):
Sample
m
ftl/a . 79 P o m . tDf-e-c. Paio, tfi P - & *
j P/tJ, fXLf - S 6 nsia. 'n i ~/ZA nun. -c
] Oa/. lfV- ~/^a
Pt/r j n f - A t fido. m f~- // Dlt, (?<)- r Pa/*}, [ `( o f - - ftNs*- A"-- C
Study Number: *!~f/t - ( 2 - 0
Equipment Number:
Notebook# and Page:
.
Final Solvent: 17, it* $ V*.
Initial <WVol.
t/mUL
ft^-i
JU&Ct'fc? pw/mL
Comments
.r/J~ JL*7
0 ,7 /* \V` I- *f.7 Y . J'C
.X9V0 J*
n./(*r .ii*s-____ ./bJ-
./or
/)>2T/20,3/-0'7 .0*70
*3 YJ s:3 /*i-
o./^r
-.J U .L I ____
O ,!(o?
.lbb
O*/bip
... <j.Y .-Q.dlib:___
rCTf) j . Y
M t*t
0*9(7 d
Y' - ./L>
J
Summary of Method:
]
I * (isti)*
Extraction*1Template Review Date______
.y
o**ft'Jf
FormApproved by/Date:. Weight Volume Sheetxls
000521
Page 1of
SOUP 020199: FACT Equipm ent Log 99014 Quattro II, #6414E
PAGE
DATE: 2/23/99
ANALYST: drb STUDY NUMBER:Tox-120
MATRIX: Rat Liver DESCRIPTION: Liver Samples
FACT Method: FACT-M-4.0 .
Injection volume: 10 uL
Cycle time: 13.5 min.
Mobile Phase:
(A12.0 mM NH4CH3CQ2
Electrode: Z-Sprav
(B).MsQH______
TN-A- or stndrd number (A) 98012-1-4__________
Prepared (date/by):
(A12/11/99 hoi________
Expires:
fAl 8/99
_________
O ) TN-A-226Q.__
(B1 2/99. hoi_____ (B )_ 8 /9 ________
Store at room temp? @ or N
Other:___
G u ard column: None _______________
Part number: na_________
Lot number: na
A nalytical column: Keystone Betasil_______ _____________________________
Size: 2 x 100 mm________ - ' . Type:_C18____________ Sphere size: 5 urn-----------
Part number: 105-701-2__________ Lot number: P8C09
Serial number: 10881106D
Analytical parameters:
Drying gas fL/hrl: 300
ESI nebulizing gas (L/hrl 15
LC Flow rate: 300 nTAnin- Backpressure: 120 bar
Sheath gas (L/hr)_Q____
EOPLC solvent gradient: Long 2
Time (min.) 110.00_____________ 21 8.50_____________
3H L Q ___________ 41 12.0
51 13.5_____________
%B_ 40.0 90.0
M Q.
40.0 Stop
000522
Tuning Method Report
Method
Cs \MASSLYNX\FACT-1.PRO\ACQUBB\PFOS
Printed:
Tue Feb 23 15:59:26 1999 .
....................................................................................................... ______________ 5P U|> 0 2 Q L W
Ot
fe / /T ?
IAC osftolti
Page 1
BESTcopy AVAILABLE
SOURCE ( ESP- )
CapillaryCone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 60 5 0.2 150 250
2.99 -60 -6
142 250
Pressures
Analyser Vacuum Gas Cell
Rdbk
7.8e-6 FAIL
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
MS2
LM Resolution HM Resolution Collision Ion EnergyIon Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
14.0 14.0 2.0 . 0.0 55 650
Set Rdbk
15.0 15.0
45 2.0 0.0 40 0 650
42
39 44
000523
Scanning Method Report
Method t
C t\MXSSLmX\PACT-l. P*OUCQBDS\TOX120A
Laet Modified: Tue reto 23 15:52x20 1595
Tinted:
Tue feto 23 16:00:42 1999
Souq fta o M
Solvent Delay < nina ) i
0.00
Function i 1
KMC of 3 Maas Pairs ( BSP- )
Inter Channel Delay ( S ec s ) : Span ( Daltons ) :
Start Tins ( Mina ) : End Tine ( Mins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell (Sees) Coll Energy (eV)
Cons ( V
1
399.00 80.00
0.25
2
399.00 99.00
0.25
3
399.00 130.00
0.25
45 45 45
60
60 60
Function : 2
MRM of 3 Maas pairs ( BSP- )
Inter Channel Delay ( Seca ) : Span ( Daltona ) :
Start Tine ( Mina ) t End Tine ( Mina ) : Repeats i
Channel Parent Daughter
0.03 0.00 0.00
13.50 1 Dwell
.
(Seca)
Coll Bnergy
(eV)
1
499.00 80.00
0.25
2
499.00 99.00
0.25
3
499.00 130.00
0.25
45
45 45
Cone { V )
60 60 60
Function i 2
M M of 2 Maca eira ( SS- )
Inter Channel Delay ( Secs ) : Span ( Daltona ) :
Start Tine ( Kina } : End Tine ( Kina ) Repeats :
Channel Parent Daughter
0.03 0.00 0.00
13.50 1 Dwell (Secs) Coll Energy (eV)
Cone V }
1
299.00 80.00
' 0.25
2
299.00 99.00
0.25
45 45
35 35
Function : 4
ME M of 2 M u a aire ( BS- >
Inter Channel Delay ( Seca ) :
Span ( Deltona ) : Start Tine ( Mina ) : Bad Tine ( Mina ) i Repeats :
Channel Parent Daughter
0.03 0.00
0.00
113.50
Dwell (Sees) Coll Xnergy ieV)
Cerne ( V )
1 2
3
413.00 119.00 413.00 169.00 413.00 219,00
0.25 0.25 0.25
30 20 30
IS 15
15
Function : 5
KMC of 3 Maas Pairs ( BSP- 1
Inter Channel Delay ( Sees ) : Span ( Daltons ) * Start Tine ( Mina ) : End Tine ( Kina ) < Repeats t
Channel Parent Daughter
0.03 0.00 0.00 13.50 1
Dwell
(Seca) Coll Energy
(eV)
Cone ( V )
1
469.00 169.00
0.25
45
2
469.00 219.00
0.25
45
3
469.00 119.00
0*25
45
,1 3300 30
Function : <
KMC of 3 Mass Fairs { SSF- )
Inter Channel Delay ( Secs ) : Span ( Daltons ) i Start Tine ( Mina ) i
End Tine ( Mina ) : Repeats t
Channel parent Daughter
0.03 0.00
0.00 13.50 1 Dwell
(Sm s ) Coll Bnergy
l.V)
Cone ( V )
1
569.00 169.00
0.25
2
569.00 219.00
0.25
3
. 569.00 119.00
0.25
45 45 45
35 35 35
unction t 7
MBM of 1 Maas Pair ( a*- )
Inter Channel Delay ( Seca ) : Span ( Daltona ) : Start Tine ( Kins } :
End Tine ( Mins : Repeats :
Channel Parent Daughter
0.03 0.00
0.00
13.50 1 Dwell
(Sac.)
Coll Energy
(V)
Cone ( V )
1
427.00 80.00
0.25
40
60
ib lAL-dlolll
Page 1
.
000S24
M asslynx - Sam ple List
020Sample List: C:\MASSLYNX\FACT-1 .PRO\Sam pleDB\022399c.SPL
Printed: Tue Feb 23 16:25:20 1999
r,
1#w. .
DU
________________________________ S n u j>
m
LAC , S /t o m
>
Page 1
R ie Nam e File Text
MS File In le t I Botti olum e Sam ple T j C one A C o n cB
1 022399020 MeOH Blank
TOX12C long2 91
10 Analyte
2 022399021 MeOH Blank
TOX12C long2 91
10 Analyte
3 022399022 Tox 120, RBL02229-H2O Blk-1,2 /2 2 TOX12C long2 1
10 Analyte
4 022399023 Tox 120, RBL02229-RBUVerBlk-1, 2 TOX12C long2 2
10 Analyte
5 022399024 Tox 120, RBL02229-5ppb-1,2/22/99 TOX12C long2 3
10 Standard
6 022399025 Tox 120, RBL02229-1 Oppb-1,2 /2 2 /9 TOX12C long2 4
10 Standard
7 022399026 Tox 120, RBL02229-25ppb-1,2/22/9 TOX12C long2 5
10 Standard
8 022399027 Tox 120, RBL02229-50ppb-1,2/22/9 TOX12C long2 6
10 Standard
9 022399028 Tox 120, RBL02229-100ppb-1,2 /2 2 / TOX12C long2 7
10 Standard
10 022399029 Tox 120, RBL02229-250ppb-1,2/22/ TOX12C long2 8 '
10 Standard
11 022399030 Tox 120, RBL02229-500ppb-1,2 /22/ TOX12C long2 9
10 Standard
12 022399031 Tox 120, RBL02229-750ppb-1,2 /2 2 / TOX12C long2 10
10 Standard
13 022399032 Tox 120, RBL02229-1000ppb-1, 2 /2 TOX12C long2 11
10 Standard
14 022399033 MeOH Blank
TOX12C long2 92
10 Analyte
15 022399034 Tox 120 DNO, 9 7F -G 1,2/22/99
TOX12C long2 12
10 Analyte .
16 022399035 Tox 120 DNO. 98F -G 1,2/22/99
TOX12C long2 13
10 Analyte
17 022399036 Tox 120 DNO. 99F -G 1,2/22/99
TOX12C long2 14
10 Analyte
18 022399037 Tox 120 DNO. 100F -G 1,2/22/99
TOX12C long2 15
10 Analyte
19 022399Q38 Tox 120 DNO. 101F -G 1 ,2/22/99
TOX12C long2 16
10 Analyte
20 022399039 Tox 120, RBL02229-250ppb-CC1, 2/ TOX12C long2 17
10 QC
21 022399040 MeOH Blank
TOX12C long2 92
10 Analyte
22 022399041 Tox 120 DNO. 102F-G1, 2/22/99
TOX12C long2 18
10 Analyte
23 022399042 Tox 120 DNO. 103F-G1,2 /2 2 /9 9
TOX12C long2 19
10 Analyte
24 022399043 Tox 120 DNO. 104F-G1,2 /2 2 /9 9
TOX12C long2 20
10 Analyte
25 022399044 Tox 120 DNO. 105F-G1, 2/22/99
TOX12C long2 21
10 Analyte
26 022399045 Tox 120 DNO. 106F-G1, 2/22/99
TOX12C long2 22
10 Analyte
27 022399046 Tox 120, RBL02229-250ppb-CC2, 2/ TOX12C long2 23
10 QC
28 022399047 MeOH Blank
TOX12C long2 92
10 Analyte
29 022399048 Tox 120 DNO. 107F-G1, 2/22/99
TOX12C long2 24
10 Analyte
30 022399049 Tox 120 DNO. 109F-G1, 2/22/99
TOX12C long2 25
10 Analyte
31 022399050 Tox 120 DNO. 110F -G 1,2/22/99
TOX12C long2 27
10 Analyte
32 022399051 Tox 120 DNO. 111F-G1, 2/22/99
TOX12C long2 28
10 Analyte
33 022399052 Tox 120, RBL02229-250ppb*CC3, 21 TOX12C long2 29
10 QC
34 022399053 MeOH Blank
TOX12C long2 93
10 Analyte
35 022399054 Tox 120 DNO. 113F -G 2,2/22/99
TOX12C long2 30
10 Analyte
36 022399055 Tox 120 DNO. 114F -G 2,2/22/99
TOX12C long2 31
10 Analyte
37 022399056 Tox 120 DNO. 115F-G2, 2/22/99
TOX12C long2 32
10 Analyte
38 022399057 Tox 120 DNO. 116 F -G 2 ,2/22/99
TOX12C long2 33
10 Analyte
39 022399058 Tox 120 DNO. 117F -G 2,2/22/99
TOX12C long2 34
10 Analyte
40 022399059 Tox 120, RBL02229-250ppb-CC4, 21 TOX12C long2 35
10 QC
41 022399060 MeOH Blank
TOX12C long2 93
10" Analyte
42 022399061 Tox 120 DNO. 118F-G 2,2/22/99
TOX12C long2 36
10 Analyte
43 022399062 Tox 120 DNO. 119F -G 2,2/22/99
TOX12C long2 37
10 Analyte
44 022399063 Tox 120 DNO. 120F-G 2,2/22/99
TOX12C long2 38
10 Analyte
45 022399064 Tox 120 DNO. 121F -G 2,2/22/99
TOX12C long2 39
10 Analyte
46 022399065 Tox 120 DNO. 122F-G 2,2/22/99
TOX12C long2 40
10 Analyte
47 022399066 Tox 120, RBL02229-250ppb-CC5, 21 TOX12C long2 41
10 QC
-48 022399067 MeOH Blank
TOX12C long2 93
10 Analyte
49 022399068 Tox 120 DNO. 123F-G2, 2122199
TOX12C 1ong2 42
10 Analyte
50 022399069 Tox 120 DNO. 125F-G2, 2/22/99
TOX12C long2 43
10 Analyte
51 022399070 Tox 120 DNO. 126F-G2, 2122199
TOX12C long2 44
10 Analyte
52 022399071 Tox 120 DNO. 127F -G 2,2/22/99
TOX12C long2 45
10 Analyte
53 022399072 TOX 120 DNO. 129F-G3, 2122199
TOX12C long2 46
1 0 Analyte
54 022399073 Tox 120, RBL02229-250ppb-CC6, 21 TOX12C long2 47
10 QC
55 022399074 MeOH Blank
TOX12C long2 94
10 Analyte
56 022399075 Tox 120 DNO. 130F-G3, 2122199
TOX12C long2 48
10 Analyte
57 022399076 Tox 120 DNO. 131F-G 3,2/22/99
TOX12C long2 49
10 Analyte
58 022399077 Tox 120 DNO. 132F-G 3,2/22199
TOX12C long2 50
10 Analyte
59 022399078 Tox 120 DNO. 133F-G 3,2/22/99
TOX12C long2 51
10 Analyte
.?
ConcC
C
Massiynx - Sample List
Sam ple List: C:\M ASSLYNX\FACT-1.PRO \Sam pleDB\022399c.SPL
Printed: T ue Feb 2316:25:20 1999
c,,
S n ,^ 0 2 D l^
ja a
U 6 S/lo/W
l .
SQf33h<f
Page 2
File Name File Text
MS File Inlet I Botili oiume Sample Tj Cone A ConcB Conce
60 022399079 Tox 120 DNO. 134F-G 3, 2/22/99
TOX12C long2 52
61 022399080 Tox 120, R B L02229-250ppb-C C 7,2 / TOX12C long2 53
62 022399081 MeOH Blank
TOX12C long2 94
63 022399082 Tox 120 DNO. 135F-G 3, 2/22/99
TOX12C long2 54
64 022399083 Tox 120 DNO. 136F-G 3, 2/22/99
TOX12C long2 55
65 022399084 Tox 120 DNO. 137F-G 3, 2/22/99
TOX12C long2 56
66 022399085 Tox 120 DNO. 138F-G 3, 2/22/99
TOX12C long2 57
6 7 022399086 Tox 120 DNO. 139F-G 3, 2/22/99
TOX12C long2 58
68 022399087 Tox 120, RBL02229-250ppb-CC8, 21 TOX12C, long2 59
69 022399088 MeOH Blank
TOX12C long2 95
7 0 022399089 Tox 120 DNO. 141F-G 3, 2/22/99 . TOX12C long2 60
71 022399090 Tox 120 DNO. 142F-G 3, 2/22/99
TOX12C long2 61
7 2 022399091 Tox 120 DNO. 143F-G 3, 2/22/99
TOX12C long2 62
7 3 022399092 Tox 120, RBL02229-250ppb-CC9, 21 TOX12C long2 63
7 4 022399093 MeOH Blank
TOX12C long2 95
75 022399094 Tox 1 2 0 ,1 06F-G1 -250ppb-M S, 2/22; TOX12C long2 64
76 022399095 Tox 1 2 0 ,1 06F-G1 -250ppb-M SD, 2/2 TOX12C long2 65
7 7 022399096 Tox 120, RBL02229-250ppb-CC10, < TOX12C long2 66
78 022399097 M eO H Blank
TOX12C long2 95
79 022399098 Tox 120, RBL02229-H2O B lk -2 ,2/22 TOX12C long2 67
80 022399099 Tox 120, RBL02229-RBUverBlk-1, 2, TOX12C long2 68
81 022399100 Tox 120, R B L02229-5ppb-2,2/22/99 TOX12C long2 69
82 022399101 Tox 120, RBL02229-1 O ppb-2,2/22/9 TOX12C long2 70
83 022399102 Tox 120, R BL02229-25ppb-2,2/22/9 TOX12C long2 71
84 022399103 Tox 120, R BL02229-50ppb-2,2/22/9 TOX12C long2 72
85 022399104 Tox 120, RBL02229-100ppb-2, 21221 TOX12C long2 73
86 022399105 Tox 120, RBL02229-250ppb-2, 2122! TOX12C long2 74
87 022399106 Tox 120, RBL02229-500ppb-2, 2/22/ TOX12C long2 75
88 022399107 Tox 120, RBL02229-750ppb-2, 2 /2 2 / TOX12C long2 76
89 022399108 Tox 120, RBL02229-100Qppb-2, 2122 TOX12C long2 77
10 Analyte 10 Q C 10 Analyte 10 Analyte
10 Analyte
10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte
10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard
000526
Method R eport
Method f i l e t L ast M odified!
Caopoundi
P rin te d !
0 1\MASSLYNX\FACT-l.FRO\HethDB\022399e.ndb Thu Peb 29 13*25*14 1999
2 t PFHS
Wed H er 03 lt* 4 1 i3 9 1999
Compound Monet In te rn a l R of.i Q uantify Tracei Cane* o f S tandardot
U s e r RP V a luei UOer Peak F a c to rt I n t e g r a t e Window F a c to r i
PFHS (None) TIC Cone. A
1.000000, Disab] 1.000000 2.000
R etention Tine (nine) > R elativ e R etention Tinet T in e Window (w ins)*
Peak Selection*
0.513. b a b ie d 0.000, D isabled 1.11?
N earest
Reopenae Typet Reoponeet
Kxtexnal . Areas
F o ly n o a ia l Type* P o in t o f Origin* F i t W eighting! A xil Transfoznatieni
L in e ar Exclude
Mona None
U n its*
Feak-to-peak asp lltu d es
b e e thingi Window s i z e ( s c a n s ! i Muaber o f saooths t b o o t h in g Method*
105
D isabled 1 2
Jo in v alley threshold*. Reduce peak ta ilin g * R aise b a s e lin e naximiwi
30.00 ? o .o o .
4.00
Draw v e r t i c a l th re sh o ld s D etect shoulders* Shoulder threshold*
10.00
D isabled 10.00
R elativ e height* A bsolute height*
R elativ e area* A bsolute area*
50.00, b a b ie d 0.00, D isabled
3.00, b a b ie d 0.00, D isabled
j
3
j
1
-
Soup t a o m
i ____ 1AC._ IS3 0 3 m
* 3
000527
Method Report
Machad f i l a i U a t m odified
Ccapeund M afiadt
CSu\iKFAa9bSM2W9C\tFfAiC2T9-*11.4TM1)9M9lethOS\022399e.adb
It NU
Wad Mar 09
2999
Cea^ouad Hanoi QICneucnaaenr,tniafayfi MTStraaaln.daairds
User W Valas Usar Feak raeters Integrate Window Faeton
JOU
IHoo.J TIC Cane. A
1.0000001.000000. Olsablad
2 .0 0 0
Rato&tian Tina (nina) i Relativo Retention Tina Tina Window (aina)
Faak Selection*
7.490.b a b la d 0.9020 .0 0 0 , D isa b le d
Waaraat
i M p c u t Typ
B etonai Arana
FfoaliynntcaadfaolrTiygpiani*
Fit Weighting Axis Tranafonationi
Unit a i
feak-te>peak a a p litu d e i
te s o things Window ais* (aeans)i Wuabar af snoathsi b n n t M n g natheds
J a in wallasf th ra sh o ld i Reduce pash ta ilin g A aiao b aaalin a naxinuns
Draw vertical thresholds Detect shoulders > Shoulder thrasholdi
A elativ a height A baolute height R elative areas A beelute areas
109
1D isa b le d
2
30.00 70.00 0.00
10.00 D isabled 10.00
90.00. b a b ie d 0 .0 0 , D ieabled 3.00. b a b ie d 0.00 D isabled
c a o i n _____ l . . - l l c h l
000528
Method Rapare
Mathod f i l a i U s e m odified!
Co^oundi
P riatadi
C t\M A IS L Y l \F A C T -l.P R O \IU efaD S \0 2 2 3 9 9 c.n d b Thu Pah 35 132514 1999
4 1 N513
tod Mar 03 K t4 1 i2 9 1999
Coepminrt K o w i In tern al R ef.i Q uantify Traeat Cano, of Standardst
U s a r XT V a lu a t Usar Peak F aeto n I n t e g r a t e Window F a e t o n
H513 ' (Mona)
TXC Cone. A
1.0000001.000000, D isab led
3.000
R eten tio n T ina (mins) i R elativa R etention T insi T in a Window (m ln s )i
Peak Selection
0 .(4 0 , Am bled 0.000, D isabled 0.730
W eareat
R esponso Type Reapc a s a i
A sternal Arana
Polynomial ly p ei
P aint of O rigini
F ie W eighting* Axis T ransform ation*
L in e ar A cclude
Hone v one
U nitsi
Feak-to-peak a sp litu d e i
IIS
A so o th ln g Window s i r e ( s c a n s ) t
Humber o f smooths i Smoothing method!
D isabled
1 2
Jo in v alley throsboldi Reduce peak ta ilin g ! R a isa b a s e lin e maximns*
30.00
70.00 ' 4.00
Draw v e r t i c a l th re e h e ld t D ataet shouldersi Shoulder th resh o ld !
10.00
D isabled 10.00
R elative height! A bsolute h eig h t! R e la tiv e street A bsolute a rcai
50.00, Enabled 0 .0 0 , D isabled
3.00, A m bled 0.00, D isabled
( A C O /o i /m
Hgt 4
g-fc
0^0529
Method Report
Method f i l e t U a t m odified
Ccnpoundi
P rin te d
C\KASSLYMX\FACT-1. PRO\MethDR\033399c.i Thu Feb 35 133314 1999
StM13
Wed H er 03 l i 4 1 3 t 1999
In te rn a l R ef. Q uantify T reeei Cone, e f Standardi
U a e r RF V a lu e i Veer Peak F actori I n t e g r a t e Window F a c to r
MS13 [Mooe] TIC Cone. A
1.0000003.000000 D leahled
3.000
R etention Tine (aim ) t R e la tiv e R e te n tio n Tine T in e Window ( n in e ) i
Peak S e lectio n i
P .S fl. tabled 0.000 D leabled
0.730
Reaponae Type Reaponaei
e te rn a l Arena
F o ly n o n la l Type F e in t e f O rigin F it W eighting i A xle T raaafo n aatio n t
Linear cclude
Feak-te-peak am plitudei
Window a l s e l a c a n e ) t tfiimhsr o f sm ooths! so o th in g atethodi
Jo in v a lle y threaholdt Reduce peak ta ilin g R aise b eaelln e m axim al
Draw v e r t i c a l th re a h o ld t D etect ahoulderet shoulder threshold
R elativ e height A bsolute height R elativ e area A baolute area
1D isa b le d
3
30.00 70.00 4.00
10.00 D isabled 10.00
SO.00, ta b le d 0.00 D isabled 3.00 tab led 0 .0 0 . D isabled
p u p . o a o m ___:___ iac m Im / m
Page S
fJtf .
000530
Method ( l l a i L ast m odified*
Ceapeunds
P rinted]
. Ci\KASILWX\rACT-l.PRO\KethDR\02239Je.mdb Thu Feb 29 13*39sl4 1999
li If
Wad Mar 03 14s41s29 1999
Cnagiraind M m i Z ntam al M f.j Q uantify Tracas Cone* o f S ta a d a rd s i
U aar RF V alas Uta* Peak Factors I n t e g r a t e Window F a c to r s
IS (Mona) TIC Cane. B
1.000000, D isabled 1.000000 3.000
R etention Tina (n in a )s R e la tiv e R atan tio a Tinas T in a Window (m ina) s
Peak O alactioos
7.410, taablod 0.000, D isabled 0.90
M earast
Rasponee Typos Raaponaos
' In tern al . Areas
Polynonial typos
P o in t o f O rigins P i t W eightings A xis T ransform ations
iln aar S a e lu d e
Nans Mans
U hitsi
--
P sak-to-paak am plitudes
IIS
ganoth in g s Window a l s o ( s c a n s ) s Msabor o f saoothss so o th in g nothods
D isabled
1 3
Jo in v o lle y thrsraholds
Raduco peak ta ilin g s R a ia o b a a o li n a maximum
30.00 70.00 . 4.00
Draw v o r t i c a l th re s h o ld s
D etect shouldersi Shoulder threshold]
10.00 D isabled 10.00
R elativ e height! A bsoluta h aig h ti R elative areas
A bsoluta areas
90.00, Enabled
0.00, O iaablad 3.00, Enabled 0.00, O iaablad
Soup.
_____ (AC j / j /m
-4
000531
FACT: DATA REVIEW SUMMARY FORM
De: '
-3 /6 5 / 1 7 ,
Analyst:
/ V ) ^ _______
FACT Study Number ~ 7 o k / ? Q
Matrix
^A r n
Time Points
______________
Dilutions?
________________
Analyte 4:
/) S / 3
Acceptable Linear Range: S~D - <7 ^ 0 ^h
LOQ for this data set: 6 W C,Q \ '
Re-evaluated for different range? ________ Diiu-li&nc httnA h r c\rp 2 *
should d .
Analyte 5:
/r?6 /3
Acceptable Linear Range: SrO -S U O /y J *
LOQ for this data set:
^4
dp'f-i
Re-evaluated for different range?
Analyte 6:
ZZ? jg_________
t>77A- OSSeAV'to*:
Acceptable Linear Range:
i-A^
LOQ for this data set:
Re-evaluated for different range?
Analyte 7:
_______
Acceptable linear Range:_____
LOQ for this data set: _______
Re-evaluated for different range?
Dole.*- (^ftnalgLL -(r fFJiS QrJ. (jilulionj
lu Gryj 3 M5/3.
000532
*r-
Zi
1
P ve
sMsssssslS
O HM M nNH h
Vi H I
II
re
tnennN tA aeH H HNM AnOIrt
SSS99S
n^hfii IWI0 9I
riri
999999
vi or i nr ivr ii -r o-
rniwn rwi nr iwona ririri
Mrn twm? imr^ihaAHaonovni rMtHMrOtir.' h4 rinn
nI rnini nniornriic*mm
ririn9
OnrnNi en<HnONrni0<r4oi
99999
co co h r* si tn rin e
nnnn
re o ri ro
H IS i
nrio)ton9
rin n h n
^o n^ nt cr o o nn n i( ^nreitrrti Vr
S iili* ri ri 1
n re cv r * ce r 4 ri ri 'f r i r i
rihriorriri riri ciroi ti
is s ili
frii
' n r r ir iN O r ir 1 a r i h w ri
M O a n ^ 'w r ir iM M r ir i
n
S iiiS S
ri re
i
n M *O V H in v i ** r* io a*
CO O r i O f# r> n n r ir ir iv
SSSSSS 1* ri 1
H r iH H r if i r ih r ir ir ir i m 19 Cri V I *9 f r i 'ri r l r l r i O
re
ii ii
S ii
ri0> 19
re i1
O r la lo OOo
r if le r ir ir iN
H
r n r O r i Vi i9 9 o ano re r i re
4 h V r ir i h r i M M J i r> a sa a ss
O M C < C r ir ir ir ir ir i w io a M i^ r i r i r i r f ri IV
tv v i v i re o v i r ir ir iV r i r i F ^ r i W r i H r i H r i VI
v i v i m in a r i
t* v i core n r*
n n is r ir iH
4 r i
H IV
re n v i v i nnoe r ir ir ir i ri ri ri ri
VI ^ VI vi H e n v i re CO V I 19
99999999
rr iiOoCwO Cr Oi or inO rriiHcViI
r i re t i
il
ii
f*i9riivro reri rivto virivivireri ^ t s rriem^r inoc on^cvcoonvr ii ovcoi
ar i ^a on irnriirrarliirWvri-rai^Or aer Oirneri r i re ri
b se eo
a R1I
e v e o o c o m v io v tv iin e o o o e o r io o e nnnnnnnnnn aaaaaaaaaa
00 00 00 00 00 90 00 09 00 m om oooooo
r i r e m ro mre ion mr - oo
9v i
e o
in re
r9
vi ri
vi n
re re r i r i o o .
999999
ri ri re
e re
r9i
ri o
r9i o
VI 9r i
VI ri o
aaaaaa
r i r i o io e
u vi
ia r
n r*
n *s
v9
r r- r r
oo
99 *
e o
o m
Oo in m
re re r i
fV t r i CDIV r i rrei n9 O* nO On iVni aaaaah
r in r iv n r1e-1^9 n0 0"V 9r i 9n aaaaaa
re r i re r** re rrei roi r9i rOi iVnI o** n o o ro
in Hr i
ritn Ar i Or i
OrcioO9o
in qr i
anaaa
Vr iI V9 r10i 9r i 9r i rei 9 VI O O 9 VI 9 f 9 9 9 r
r9i
99
9 ri tn r i
O VI VI VI
9 r* r* F"*
Vi Vi Vi r9i VreI VreI 9 r* f*
9e
o o
oo
o o
o o
oo
o e
ri rio
in re
9e re
o re
o ri re
o
9. o n tn
re re re
e r i'V f'ririre rirc ri re riririririririv i e e e io c t o c ic ie c i 9 9 h n F > F 'a r
000000900 o o o eeo o o o r i roi nr i roi oo no oo i on oo
r i re in r* o
n_
CO
IO o o o
mm_ .%%%%
Jj X i V Al sssss ssssss ssssss ssssss s a s s i slisss
IllIlllllIliIllIlSalialalllllalIllsIIflIlB lIlIilsIIIlslIIslillslllsl
9 a
ftei
Ih
I
ri re vi
v i re
vi vi vi vi w
s s rimririririris
n n vriririssssn res ns snsnsnnnnnnnnnns
n rin n n n ssssn
in sssn n n n
M snnn
n hn
* *n
i ri n n n n i
M te n n n ;
V tii
3SSS o o o o o H Kn Mr iDHrrai rOi rr iinOr ri hi rOi
nVI InV InCI InVI lnVI lnVIlVnIlOnIlOnIIVnIIOn II
nnnnnnnnnnn
3n nO nQ nO nO nO nO nQ nO nO nO
J JJ J
J JJ
SSSSSSSSSSS
HOOOOOOOQOOO
R nr i nr i nr i nr inr inr irr ii irii rr ii rni rni
mmnm
1
i
9. a
i
evni 0
1
* 1 P > W ! p o p n * 3 T 4 < TM nfc ' T O Z O n o
ssassassssssssssasssssaaaaasssassxassaasasasaassssayaKasassssssKassKssssKKaaaas..|
C/SE/Z
ltd Coaa
lasaiass sssssssss
iiiiiiiii
a a ss
a s
9tAt A 4*1
ssi i ii i
a s
o
i sil
o o
lIlsS a s
o
en 5ssSssco
CO US5SS2SS
> MUwm M<40
iiiiiiiii
Mw i s as IS s. h t
asa 5 oo So
sbs a ss ss
oi
A Ul A
i
* M
ggg g gg SS
o So i asa
i w w
g . ggg
a s * a
1
s
a i,
Kl
g
aa 5s
A * *1A A i a ii i
a 3
<4 A
si
I a as i
5M o oo s SS a
1i
W
a
H -*
g g gg g
s S
o" sa
t
* -
gg
a s
A A*4
i ii
3 ; ' ISsaSsKs s sssssssss
rra r r r r r r r r r r r ats s ssSassScsS
3
i as
S So S SS 1
u A
g gg
l is a Il3ll=HS
sao ass 1 HA
ggg
A asassaaoa u asaassssas
i i iw
III
1 MMW
^wHfcotoau u
g gggggggggg
*
s
i ss
L
s53s:;2ss?ssss:a2s^s23a522E?ssa3SS2i5Bsss:5:s;r;sssts;si:sKt:sss3SSiiSK;t:s;s5:sKts---i.u.^l.k.>..
iSSiieBilBfiBBeBBBeEggSBBeESBBSeSBeSISISBBiSSSBIBBBSiSiBSISSBSBBBSSBSiillil 1
sssssssasssssasssss S3SS2SSS1SISSSS2|S
iilliiiiiiilllifiiiliiiiliiiiiiiiliiiiiiiiiiiii V
1 !p
!1
fofff Si
mmmmmimnmmimmimmimmimmmmmmnmmmmmmiii
g s S s S S S S s S e S S S s s S s S S s S S i ^ S s s S s S e S s SPSSSSsSfSJSS5SSSsSsssSgSSSSsSSSsS^Sg^SSSsSSSgS8^g3SiSS!
[{ If
k ks
S
S
S
i;lSlSiSisi-lilTSs5
l'*nn\nn'*ni'znn'*nw
5|gS p p g p s p p i s m
}352
f
sss sssss sbbhb ssf
i
s
if# I#i sssssSiih; , ssSs SSSS"S
ms
2r3!
i ili!
l,,...
acLa^a^P-P.
issalsasu. sskissssi
B
ss ss
*4
S
Sg Ss
sgg sss
4ggi4<) *4g
i g g g g a
S S2 SSHS
S W' 5
O O O SSsSSa i=l i C/T CO S2K5S3S C/ sbbssisb
* ***i*
gglIVIII.
i HB s a a s s s 3s
S SS SsSsSS 53 g 36 833358 S3
s sSSs ssSSsS S 3 .2652 SSS325 5 855522 256258 6
g igsgiggg s 2SSSSS8SS
IgiSasgs P SsssSts. S 8355SS83
ggssssss s?
swm
Soup 02019911 qunatltntod and printed fay m 3/25/99
saasasasssssssssasssaaassaapsssaxssaBBgsasaaissasssygKiiaKSgsssKKSSKSEiitJi:!: rasas.,
ss :sssi:lli| | | ll ll! il l ! | ! ! i| ! i ! {
ssils 1 i
S 3 Isss
i
mmmmnmmmmnmmmnmmmmmnmtinmimminmmmii
ESSSSESSSSESSSBSSSSiSSSSSSSSSSSSBSSSSSSBSSSSBiSSSSSBSIiSSSsSSSSSSsSSSSSSsSSSSSSSSSSSsg
;'B;v3'I;':',;'B(',;;i;!(ii;!)ii!i;iiiiii'eiS88!E'c|j|ggrl!gi|{mipgi:igsiiiegg|g|iS8 g s g g h e.B.SSEi:iS(i
miiiii i l l l l l lilis lili!
il
I
.U :hikh
l ili p i l l i [ H i l l
SS PsSSSS "sssss
Usi
m
i? m iiiiiffii
SSSS
Is ls s s s s . S .g g g g s s g ss'sssssss s s s s s s s s
iiiiii
SS i i i i i i iiiiSS SSS3 i i gsisss
asaSs
kvs
sksbss
SiidiK gXXXXX
tU BaseSssssE SSSssii ssbI ss slssSa s l l s S
SES ssSs s iiS 55sls s s l l l l S lls s s c l l S I l S
S 3 3 SSSS S S S S S S SBSSBK S3XSSB gCSSKK S B S S S S a
LL
C
3
s
SX? XSXS XEKXXX H f f B W ififSSSS ISSSXS XSXSSS
uI> S
3r3vc
stsSssssssssssss lililili
jj ! s
SSSHssas
sasssago sssasass .. a
H I M h* U O OMMMOHHO
gBSSSESS
S
e ?
f <
I
s
s I
H*
*I*
t
R& ? I
oooooooooooooooooo
>000000000000000
oooopopopppopppppooopoooppooooooooo*
tMOl OI-OOlMVOIHO OH> WOMOWMiHOHOt f0Vla-W<lMOAO)I0IOIV^Ol(iOei MWO OHi OeO(Ol(tllO*( l>OgaO>nUOMIOM OUa iMOl COHlOO^ >IOOJO>OJOI>OjlOAi 'UO>IOl ^>JOWs lO*MJOH<O4OOO)(OOt >tOJAOOAi UOOIO(#AlOUAOMAOAHAOOUO* IOOUIp,>UO*(JOMOpIlIVU^IWU lMi fHl U^OUI(l *^ J O t Of kOl t Ol kO^ fOk ^OWOWOKOWOWOWOWOWOWOWOlOi l OWOMOMOMOWOMOMOMM
i m t2S33g3g?38
XXXXXXXXXXX
Sx ix?!i
Itm
aa
P H H H H H M H H H r - H -** M N N IMIMN N IWmV MN MN N Vp
V4HMMH
OOOOOOOOOOOMOOOHOOO
*
>> HX0*
HH
O
0O*
HHHH WMMM OOOO
0 MUMHWM
HOOO
HN MH
OO
HM
O
0 MhWw
HOO
Mm
O
mU
O
i m m i t m m m m m m i m m i m m m t t ia as HPHHHH.
** "*
*m
0 K1N MMMW0
HM Hb MHWHHU HMHMMWHWMW X X mf
HOOOOOOH
O H O O O O O M O O O O O O H O O O O O O H OOOOOOOOOOHMH
ssesssesse* PSIM0 r 'Oh Oh MOOOf t0Op0Op 0Op 0Op OtO' o $ Q ! l l ! l I? l i l i ^ i i n i t ' i
IsS iiS il
MU MMMMMMMN1.N....N....M.....M.....M......M............M.....O.....A.
UMMMMNMMMMM MHH
t0 IOWA
O I I
I III Ililil
I MM
s s s ls S S0 8SH4MM
ow ovo
K
S
t
f
g
i
S
MUM MO O
O OOOP
IW Wvr7?-*s& I r r1 MNN* HK
EssMIM W N* W*
M H, .M n o *
NKHKHH MWUWUU MWU U U U
bu ao ub WHU SMSM*Uv MMW Ws
iUt Ua ka l Up Uh h l .
01 f o uowwww
o* * *
MNMMM &SSSSH T mummw nWMMMN fJSNSSS
3 MNWUM fi' N S N S V i mmmmm nQMNMsMs Ms Ws
mhhm MMMM ----W-- sl*O- tWU
ssss 0* * * S UN W D, ' * ' * S ( UUM OMUM O *s. *>s*s
MMNMHH KI MHOt t M a *8* *8*8*8 lilil MflOQA
Uoi*UMM*N*n
fWMMNM SSVSV
NMMMM qmwijmw
m ili iiiiii
8k .s..s..s..s..s
vMWMMM 0` s . S V S S 1 MMMNM nUMMHM
UMM-S Jf* MMMM N N S M I
H* O 01
aaa aaa
aaaaaa a a aa a
3a a a a a * aaaaa
a a a a 100 WV aaa
W ' ^ ' . S S WWNMHW MS
SMWMM MMMS< N
SN
MMMMM s s n m m MMW
NSNSV. U V t f 4 NM
s . UWt A<0 S N
(Al AtttfV
Mtt Wt f t t N
Mi VIA
10
S-
Ui HfHf )HH>
MMMW . 's s s s I MWWM UWIAI d
l lfp ig l
g* - `
W>0MHoHaHH
s
MM OHOHWM *tlt
1 Ii ooo MOflHHH WH H* *
OWOMOMOWOHONMOMO WD OWOU
MMMMMMNMMMM
tMIS tNIf tMI0 tMId lMI NI0HWI MUIM>IVM0I MWI
oH *u JiUoMu HoUoI uWpHiU U) 8D
0 0 Ooes S S Ur 0
fMMMMM
SSSXS MMMMM " " MMW
MUM WMsvs NNMMM
Tf HHH HB
W0WI0WOWV0W
iMMMUU i htWi MS N ' f c
WWm * WMMUNVSMI
r t N \ MSSSSMMMNHH
i SHMMNMMNS*s
MHWMWSNN H
M S S ^ N VIOWIAMM
Su)0I' O100tyfWWtf
S\ MIA
10 k) 0
IS
ff
l B55 *1 I i fl
O o o C/l co
OMUl WM OUI OUOI MI H OOOOOOMOI l
I 0
^ IX* XV XV Hf V* XX* W+ ,
H*J
^ )*jnui
0M ew ve m
#10
tAWt0
UWW0 a a 1 01 M0 0 l
0 10 10 10 10 10
*U 0> 1 -j 0 j (0
10 10 O(0 VA
01 aae nafa
10 10 1A10 <0 10
10 10 10 10 10 10
K>H H Of JHWMH I OM0 0 A 0 W0 0000000110
y
a
tauiu a m h u m m
u u j
SSn
0
aa
amow
u0
00
wwUaSSS
u h 0 h a v m m m u m n w m m w w m u w u 0
0ij0o0O ow hohm
0 h
0 h
u
0 oi
u
>
m
a a 0 w i 0
h ma a mm
0 00 0 h 0 mhwmou
**
<4
HA w- Ig0AMHHU N UttHURMHa BI WB^WWWWI -
MWU HHU WW*J
si | nI
0^0
11
y y
B 355
0*100 5m *h
|J *4 -
533B
HUUJJ o SSShm
|
5&5&&B
sj*i0B o w tta w u w u S a o CawS*-*
1 "'`'
W H SI .
5 & m & B3B5B5
0 H HM
aaoaoo H0MWW0
0 0 0 UWU aoaaaa
UHHHHM
MHH0MO1 a a w 01 0* si
0*1000
mooumou
M
m KI
m
1
I M
Ix a
M
5 & m B BB&B&B &
I a
?
Ooo iuol Uoo aNo oHoaoMa Ho t
aaaaaaa
I000HMOAW0 WHI
oaaa aa c
0a iHal UHa MuUHw0 0s i
53 g g m iv
3
s
Is f m f H H E H H H H I H I H Z E H H H IOUHIUIMHMMIUBfI m m n m \Mff
0
WH MH MP MH MK HM t
HOOOOOOi
MM
MH UM D-MM MM 90 0 0 0
HhHHHM HOOOOOOH
UMMMVMMMMM
OOOOMOOOOO.
immi
nMuOtjOMOMtOjOtOlMlMe oWoMoMeMoMeWeMoMoWoMHtIHVH*
&
I 538883'oMp Mop Mro MPo fWo rMo 1& MKl(IMiMlNWHtiMUHt-lMWWgtMMgt
g PO
?
! JsJMnMUNMMvMSMMMnlSWJMMNI's4 4 4 4 M MM MNN
IJPPMHH h ro*m \
UN UoWl
QWU4HiAWUOl1)WUi*^O0lUU*mf
Uw Wl*4
<mMM0Uk*MUhjIWhM*bhH*l*hOU*
m w Sww
IMMWMM
I MMMMM
8;
f'llSII1 m H m h i i
ligliUli .................... &
S ............
M M H O 4 (B
.UJfnIIfMMNS 4SMM4MNS4IM4MNS4IM4MSV4M M4USN4M
ioMiHuiHmIi I " * "
I0uJdi")m"mi"lm "Cm1 S
1 MUMM
kM
MMM O
HHp H
HPO i
HO4 n
HO4 *
Wf)f>
MHH
441IMMM<4AOO41U1AO*I*1
*> MOMOHOH4444-J
*I MVD*H4 *044i4 < 4I i M2n
M *JUl |J H W
0 m o ut o o m <
65I`SMN.MMS.MMX'MMs
8MWNM
W 4 4 4 4
I MMMNN
Qtro'crtri iY--*
MN*SI M M I N4 Nir'
aT
* M
M4UNH 4MMS4 MS44 WSW4 WS44 4
o* Y Y Y Y * * * * * * * pe
A1n*M*
-* M
* * M
** H
***
NM NM
*
SM
MsMKIHH*HI
SmMMS4
MN4M4nM4V4WxHK4Ms4MsV44MMm44NMm44VM
ms44>
.n44
*h MSM
MmMSVMI
ta uui
tii n
* !i3S?3li~R *Ss1g* t n n si
H 4 M ? *?fi &W IwJjKMjH *mBKH f ?3i3 SI ? S??88 3
A HHMH
:44- m4:"^ggio4:m"g?o1^sr 8
gigggigggSi?si!i8E B E si n n
B.&Z
UuO MOm MO4 WO4 WO4 MOU MO4 MO4 MO4 oo oo oo oo oo oo oo oo oo
Mme Moo oo- `oo
MUOl oo
Mmo oo
MUOl Muo oo oo
4 -Jsi>1 >41-1 4 >J1 4 4 0 M4A WMWUMUl tMWtM4A *MWlMUU IW4WHI AW0 MUOA E4 4S 4K
4 4 4 4 <4 <4 <4 J <4 *4 1 4 <4 *i4 Ni 4 *4 Ni <4 nJ <4 4 4 4 4 4 4
MU4 NWIN44 W4K) WmVSWmlW4m MOu W4u H4
U0i1,tU0JUMUWUMUWMB HUU
UMm
Mw
a
WuWH4Wwa*
e>
MHU SMU UUMMWMIUMi UMM
4iOd OO
444444 MUW MU4 WMU MU4 UMH AHO
MMO UMOl' Oo oa
44444 uIA I^wU4uUUKwIImWU
444444 MW4 UWM M4WUWMUMMf 4Ut
WMO
oo .
444444 U4A WHA lMUiMPW4NA 44U
MIodUMol MoU MoW MoU MoW MoUlMolMou oo oo oo oo oo oo oo oo oo
44444444444 M4 oMmWmUm mW mOw WvUoIiUoMw*>
o o o
CO 00
44A44R404UW 4 AA4UIHI0H AW4 OURHORU4IIIHM
4ldU 4 AO OI Ni O
44 AW 4AU04U OO140* 4 N 4 U U U H 4 Ow A A A l i AW
a m o m a U 44ROAU MUt O011410 WAUUO OUl HO WU OOOAAO
A A4A4W WOA A A AOOB4
400400 UMU0UO
O4H004
O O<4O14 AOUH0 OWO 140i
4 0 O0 0 W W4 W4 IdA HOWOWA
M 10O1010t001 OH M0 4 O 001410 i ill
4 4 4 4 4 0 4 4 AMM OA 4 AOUIOA0ON A MOOAWA A 0 0 A
NMMMMMMMIJ M mmoummui*W I44AWW4UI0
aMOUl>M0W>JOUA W04H04RmAAH
MH 4MOO H 1410 0400 HAU
MMMH MNOH 4 0 AH
00 WOUO
NMHHH UOM0UH HO4AO0
'4 ' 4 n ( ll W0 I H4 UA
1 1h
i
11 1 OIR
ohoiuw aw rhw m
11 MO HAO
55555555555 555
f1 1 1 HU N U
5555
III
1
HMN 1U
HNUHU0
555155
MWMUHH 04UB0M H40U0A
HWNO0N WAOUWA
1 II MH H H OHAuao
515558
HWMUMH U04HAN O H W HMUHWH HAUHU
i O H 0 U H
555555
4MHMMH 4H0H0M 0UOOUA
U4004M WH 4 A O4
0J01010) 0i M A A4A4W W0040O
00OOH WH4U00
MWMWH *4Ul A 01M 0 1A011414
0 OU A 4 0 4 OOA
MM14M01M W0O04H 4 WK) WA 0
o o Ino *i o OWOiNOH
141401WW01 MMOMHM H 4 O0 A W
O010101014 0 4 0 AA
NMMMMMMMM W0W0W4WAMO4 4 M A4A0HOAM
MHbwAAW0A4A AHW0WWMA4AA
111 H 1H 1A B H A A MO
555555
1 H l H W40HH
555555
1 MOI Ut 1 HWHHU MMMUt O u A H A H 4
55555 555555
1 0>140*0H CB* * o ON| o
555555
111 1 1 M 1H A4 uhhhuhw w m hh
55555555555
3
g
Q u a n tity C a lib ra tio n h a p o rt T e n 1 3 0 EN0> S a t L i v e r B c t r a e t a
C a lib r a t io n : C: \KUat.not\rACT-l.T*O\CurvaI\022391a
Lent nodlfiad: Thu yak 23 13:43:3* lift
t r in t a d i
Thu Tab as 13:13:33 139*
lag s 1
Q u an tify C a lib ra tio n la p o x t T o x 13 SNO, K a t U m e x t r a c t a
calibration! C:\MuMMllX\ncs-l.mo\CurvaPa\033399a
tant aKxlliadi O rob 35 13:43t3l 1
rrintad!
tbu Tab 35 I S iMtSS 1555
ay a g a
Soup 0301951 > q u a a tlta ta d an d p r in ta d b y a a a 3 /3 5 /9 9
000540
Q u a n tify C a lib ra tio n JU port S e x 1 2 0 ZWO, b a t t i r e r d e t r a c t a
Calibrations Cs \MLBSLYHX\raCT-l .n o \ C u r r e C B \ 022399e
Last nodifled: Thu Feb OS lOtiOsOS 1999
Tinted!
Thu rab 25 13t43tS5 1999
Soup 020199t! q u a n tita te d an d p r in te d b y n e e 2 /2 S /9 9
000541
Q u an tify C a lib ra tio n lo p o rt T e n 1 2 0 OHO, R a t L i v o r e x t r a c t s
Calibration: C: \IOJiLa\FlCT-l .FR0\C u m B \ O 2 2 3 9 9 o
Last aodiiiod: bn Fob 2S 13:43:3 1999
Friatsdt
fbu Fob 25 13:43:55 1559
Soup 020199:: q u o n tita to d an d p r ia to d b y ano 2 /2 5 /9 9
000542
Q u a n tify C a lib ra tio n lU p o rt T o n 120 SNO, B a t t i r a r B n tr a e ta
Calibrations C: \XxaSLYt0C\riCT-l VftO\CorYal\02239o
tarnt aodifiads T bu fab 2$ 13:43i20 1999
Trintodt
Tbu Fab 2$ 13i43sSS 199
p as% 5
Soup 020199s * q u a n tita ta d and p r ia t a d b y n aa 2 /2 S /9 9
000543
Q u a n tify C a lib ra tio n X aport o n 1 3 0 SUO, H a t U w a t t r a c t s
callbrat Ioni Ci M o a s u n a i r a c z - l . m o \ c u m \ 0 3 3 3 33e
Last sndlfladi Thu Tab 35 U i U i S l 1*5*
Prints*:
Thu rsh 35 I3i43i55 15
paaa f
Soup 0 3 01$S :i q p ia n tlta ta d an d p r in te d by
3 /3 3 /3 3
000S44
Draw v t f t i e a l e lu ru h o ld t Daeaet ahouldara i flfcouldar th resh o ld
10.00 D isabled 10.00
0eI.eS0eSSc
S iS i
m IT IT
*e o o* oo
/
00054S
M i t t'St'tt St K U nwBtccttoNKnnHvmi'i-xsviMnoivniiX'a
l*TJTP" I t u p r *
od.* poinw
SO U P 020199: F A C T E q u ip m en t L o g 99014 Q uattro II, #6414E
P A G E _______
DATE: 2/24/99
ANALYST: drb STUDY NUMBER:Tox-120
MATRIX: R at L iver___DESCRIPTION: Liver Samples .
FACTM ethod: FACT-M-4.0 ,
Injection volume: 10 uL
Cycle time: 13.5 rflin.
Electrode: Z-Sprav______
M obile P hase:
(A)2.0 mM NH4CH3CQ2
(B).M s.QH _____________
TN-A- or stndrd number (A) 98012-1-4
___
(B).XN-A-22$Q_____ ____
Prepared (date/by):
(A) 2/11/99 hoi________
(B).2/29, h o j ______:____
Expires:
(A) 8/99______
. (B)_3/22_______________
Storp at room temp? or N
Other:---------------------- -------- :--------
G uard colum n: None________________ i_
P art number: na______ __
L ot number: na ________ ;____________
A nalytical colum n: Keystone B etasil
______________________
Size: 2 x 100 mm_______________ Type:_lS____________ Sphere size: 5 um_________
P art number: 105-701-2__________ Lot number: P8C09
Serial numbcr:lQ8811Q6D
A nalytical param eters:
Drying gas (L/hr): 300
ESI nebulizing gas (L/hr) 15
LC Flow rate; 300 ut/m in . B ackpressure: 120 bar
Sheath gas (Uhr)_Q_-----
H PLC solvent gradient: Long 2
Tim e fm in.) D000 2) 8.50
3) 11.0
4) 12.0
5 L 1 3 J______
J& 2-----------------M Q ___________ JLQ______ :____ 90.0___________
J fl.iL -_________
Mm_______
000546
Tuning Method Report
Method:
C :\MASSLYNX\FACT-1.PRO\ACQUDB\PFOS
Printed:
Wed Feb 24 15:22:59 1999 ' r
..
____________ _________ Spuj._&a.0M__ lA<L QS/fofo
Page 1
;,w r m w u
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 60 5 0.2 150 250
2.99 -60 -7
.150 250
Pressures
An*alyser Vacuum Gas Cell
. Rdbk
1.2e-4 5.2e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
MS2
LM' Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
14.0 14.0^ 2.0 " . 0.0 53 650
Set Rdbk
15.0 15.0
45 2.0 0.0 40 0 650
42
38 42
000547
g c u n i n g Machad lupore
Maehodi
C t \ M M * u a c c \ n c T - l . m o u c a o s a \ T a x l 20A
L u e Modifldt Sua rab 33 10:01:03 13*9
P r i n t ad:
Wad F*b 2 15i23:l( 1399
elvant Balay ( ala ) i
0.00
Function t 1
MRM et 3 M a u Pair. ( Xlf- }
Inter Channel Delay ( Sees ) x Spaa ( Daltons ) t Start Tine ( Mias ) s Sad Tiae ( Mins ) i
Repeat* * Channel Parent Daughter
9.03 0.00
0.00 13.50 1 M l (Se.) Coll i n r g y
(.V)
1
399.00 30.00
0.25
2
399.00 99.00
0.25
3
399.00 130.00
0.25
43 45 43
60 60 60
<V.)
Function i 2
M UM of 3 M u a Fair. (. ZSP- )
Inter Channel Delay ( Sees ) i Spaa { Daltons ) t Start Tine ( Mias ) i Snd Tiae ( Mins ) x Repeats
Channel Parent Daughter
0.03 0.00
0.00
13.50 1
Bull
(Sees)
Coll Bnergy
* (.V)
Cone ( V J
1
499.00 30.00
0.25
2
499.00 99.00
0.25
3
499.00 130.00
0.25
43 45 45
60
60 60
Function i 3
MUM of 2 M u a Paira { SSF- )
Inter Channel Delay ( Secs ) t Span ( Daltons ) s Start Tine ( Kins ) i En d Tiae ( Mins ) *
Repeats i Channel Parent Daughter
0.03 0.00 0.00 13.30 1
Dwell
(Secs) Cell Energy
(VJ
Cone { v 1
1
299.00 30.00
0 .2 1
2
299.00 99.00
0.25
43 45
33 35
Function s 4
M XK of 3 1toss Fairs *{ ESP- )
.
Inter Channel Delay ( Sees ) i Span ( Daltons ) Start Tiae ( Kina ) x Knd Tiae ( Mins ) i
Repeats Channel Parent Daughter
0.03 0.00
0.00
13.50 1 Dwell (Sees) Coll Energy
(.V)
Cone ( V )
1
413.00 119.00
0.23
2 413.00 169.0 0.23
3
413.00 213.00
0.23
30 20
30
15 IS IS
Function x S
HIM of 3 Mess Fair* ( M l - )
Inter Channel Delay ( Sees ) i 0.03
Span ( Dalton* ) *
0.00
Start Tiae ( Mins ) t
0.00
Snd Tine ( Mina )
13.50
Repeats t
1
Channel Parent Daughter Dwell
(Sees)
Coll
Energy
(.V)
Cone < V )
1 > 469.00 169.00 0.25
2
469.00 219.00
0.23
3
469.00 119.00
0.23 '
45 45 45
30 30 30
Faction i 6
MRM of 3 Mass Fairs ( ESF- 1
Inter Channel Delay ( Secs ) x Span ( Daltons ) t
Start Tiae ( Mins ) i Snd Tiae ( Mins )
Repeats i
Channel Parent Daughter
0.03 o.ao 0.00 13.50 1
Dwell
(Secs)
Coll
Energy
t.V)
1
369.00 169.00
0.23
2
569.00 219.00
0.23
3
369.00 119.00
0.23
43 43 43
Cone C V )
33 33 35
Faction i 7
M R M of 1 Xkta Fair ( tflf- )
Inter Channel Delay ( Seca ) i Span < Dalton* ) ,t Start Tiae ( Mina ) t End Tiae ( K in s ) $ Repeete <
Channel Parent Daughter
1 427.00 10.00
0.03 0.00
0.00 13. SO
1 Dwell (Sac*) Coll energy (eV)
0.23
40
Cone ( V )
(0
lAL or/hli
000548
'*kr:
>>n 1
M asslynx - Sam ple List
Sample Ust: C:\MASSLYNX\FACT-1 PR O \Sam pleD B \022499a.SPL
Printed: W ed Feb 2 4 1 5 :2 5 :3 9 1 9 9 9
_
,,,
/
Soup P1QJ31. JAC Ql/Io
Page 1
F ile Nam e File Text
M S R ie In let 1 B ottk olum e Sam ple T j C one A C o n c B
1 022499001 MeOH Blank
TOX12C long2 91
10 Analyte
2 022499002 MeOH Blank
TOX12C long2 91
10 Analyte
3 022499003 Tox 120, RBL02229-H2O B lk -1 ,2/22 TOX12C long2 1
10 Analyte
4 022499004 Tox 120, FtBL02229-RBUverBlk-1, 2, TOX12C long2 2
10 Analyte
5 022499005 Tox 120, R B L02229-5ppb-1,2/22/99 TOX12C long2 3
10 Standard
6 022499006 Tox 120, RBL02229-10ppb>1,2 /2 2 /9 TOX12C Icng2 4
10 Standard
7 022499007 Tox 120, R B L02229-25ppb-1,2/22/9 TOX12C long2 5
10 Standard
8 022499008 Tox 120, R B L02229-50ppb-1,2/22/9 TOX12C long2 6
10 Standard
9 022499009 Tox 120, RBL02229-100ppb-1,2 /2 2 / TOX12C long2 7
10 Standard
10 022499010 Tox 120, R B L02229-250ppb-1,2 /2 2 / TOX12C long2 8 '
10 Standard
11 022499011 Tox 120, RBL02229-500ppb-1,2 /2 2 / TOX12C long2 9
10 Standard
12 022499012 Tox 120, RBL02229-750ppb-1,2 /2 2 / TOX12C long2 10
10 Standard
13 022499013 Tox 120, RBL02229-1 OOOppb-1,2 /2 2 TOX12C long2 11
10 Standard
14 022499014 MeOH Blank
TOX12C long2 92
10 Analyte
15 022499015 Tox 120 DNO . 145 F -G 4 ,2/22/99 ' TOX12C long2 12
10 Analyte .
16 022499016 Tox 120 DNO. 146 F -G 4 ,2/22/99
TOX12C long2 13
10 Analyte
17 022499017 Tox 120 DNO. 147 F -G 4 ,2/22/99
TOX12C long2 14
10 Analyte
18 022499018 Tox 120 DNO. 148 F -G 4 ,2/22/99
TOX12C long2 15
10 Analyte
19 022499019 Tox 120 DNO . 4 9 F -G 4 ,2/22/99
TOX12C long2 16
10 Analyte
20 022499020 Tox 120, RBL02229-250ppb-CC11, i TOX12C long2 17
10 QC
21 022499021 MeOH Blank
TOX12C long2 92
10 Analyte
22 022499022 Tox 120 DNO. 1 5 0 F -G 4 ,2/22/99
TOX12C long2 18
10 Analyte
23 022499023 Tox 120 DNO. 151F -G 4 ,2/22/99 TOX12C long2 19
10 Analyte
24 022499024 Tox 120 DNO. 152 F -G 4 ,2/22/99
TOX12C long2 20
10 Analyte
25 022499025 Tox 120 DNO. 153F -G 4,2/22/99
TOX12C long2 21
10 Analyte
26 022499026 Tox 120 DNO. 154 F -G 4 ,2/22/99
TOX12C long2 22
10 Analyte
27 022499027 Tox 120, RBL02229-250ppb-CC12, TOX.12C long2 23
10 QC
28 02249928 MeOH Blank
TOX12C long2 92
10 Analyte
29 022499029 Tox 120 DNO . 1 5 5 F -G 4 ,2/22/99
TOX12C long2 24
10 Analyte
30 022499030 Tox 120 DNO. 157 F -G 4 ,2/22/99
TOX12C long2 25
10 Analyte
31 022499031 Tox 120 DNO. 158F -G 4,2/22/99
TOX12C long2 27
10 Analyte
32 022499032 Tox 120 DNO. 159F-G 4,-2/22/99
TOX12C long2 28
10 Analyte
33 022499033 Tox 120, RBL02229-250ppb-CC13, TOX12C long2 29
10 QC
'
3 4 022499034 M eO H Blank
TOX12C long2 93
10 Analyte
35 022499035 Tox 120 DNO. 1 6 1F -G 5,2/22/99
TOX12C long2 30
10 Analyte
36 022499036 Tox 120 DNO . 1 6 2F -G 5,2/22/99
TOX12C long2 31
10 Analyte
37 022499037 TOX 120 DNO. 163F-G5, 2/22/99
TOX12C long2 32
10 Analyte
38 022499038 Tox 120 DNO . 164F-G 5, 2/22/99
TOX12C long2 33
10 Analyte
39 022499039 Tox 120 DNO . 1 6 5 F -G 5 ,2/22/99
TOX12C long2 34
10 Analyte
40 022499040 Tox 120, RBL02229-250ppb-CC14, TOX12C long2 35
QC
41 022499041 M eO H Blank
TOX12C long2 93
10 Analyte
42 022499042 Tox 120 DN . 166F -G 5,2/22/99
TOX12C long2 36
10 Analyte
43 022499043 T o x `120 DNO . 167F-G5, 2/22/99
TOX12C long2 37
10 Analyte
44 022499044 Tox 120 DNO . 168F-G5, 2/22/99
TOX12C long2 38
10 Analyte
45 022499045 Tox 120 DNO . 169F-G 5, 2/22/99
TOX12C long2 39
10 Analyte
46 022499046 Tox 120 DNO . 170F-G5 2/22/99
TOX12C long2 40
10 Analyte
47 022499047 Tox 120, RBL02229-250ppb-CC15, TOX12C long2 41
10 QC
48 022499048 MeOH Blank
TOX12C long2 93
10 Analyte
49 022499049 Tox 120 D NO . 171F-G5 2/22/99
TOX12C long2 42
10 Analyte
50 022499050 Tox 120 D NO . 173F-G 5 2/22/99
TOX12C long2 43
10 Analyte
51 022499051 Tox 120 D NO , 174F-G 5 2/22/99
TOX12C long2 44
10 Analyte
52 022499052 Tox 120 DNO . 175F-G 5 2/22/99
TOX12C long2 45
10 Analyte
53 022499053 Tox 120 DNO. 177F-G 6 2/22/99
TOX12C long2 46
10 Analyte
54 022499054 TOX 120, RBL02229-250ppb-CC16, TOX12C long2 47
10 QC
55 022499055 MeOH Blank
TOX12C long2 94
10 Analyte
56 022499056 Tox 120 DNO . 178F-G 6 2/22/99
TOX12C long2 48
10 Analyte
57 022499057 Tox 120 D NO . 179F-G 6 2/22/99
TOX12C long2 49
10 Analyte
58 022499058 Tox 120 DNO . 180F-G6 2/22/99
TOX12C long2 50
10 Analyte
59 022499059 Tox 120 DNO. 181F-G6 2/22/99
TOX12C long2 51
10 Analyte
\.
000549
Masslynx - Sample List
Sample Ust: C:\MASSLYNX\FACT-1.PRO\SampleDB\Q22499a.SPL
Printed: W ed F eb 2415:25 :3 9 1 9 9 9 ,,
ni..e...l..l...i.
lA c.nsll.lv
Page 2
File Name File Text
MS File Inlet I Botth olume Sample Tj Cone A ConcB ConcC
60 022499060 Tox 120 DNO. 182F-G6 2/22/99
TOX12C Iong2 52
61 022499061 Tox 120, RBL02229-250ppb-CC17, TOX12C long2 53
62 022499062 MeOH Blank
TOX12C long2 94
63 022499063 Tox 120 DNO. 183F-G6 2/22/99
TOX12C long2 54
64 022499064 Tox 120 DNO. 8 4 F -G 6 2/22/99
TOX12C long2 55
65 022499065 Tox 120 DNO. 185F-G6 2/22/99
TOX12C long2 56
66 022499066 Tox 120 DNO. 186F-G6 2/22/99
TOX12C long2 57
67 022499067 Tox 120 DNO. 187F-G6 2/22/99
TOX12C long2 58
68 022499068 Tox 120, RBL02229-250ppb-CC18, TOX12C long2 59
69 022499069 MeOH Blank
TOX12C long2 95
7 0 022499070 Tox 120 DNO. 189F-G6 2/22/99
TOX12C long2 '60
71 022499071 Tox 120 DNO. 190F-G 6 2/22/99
TOX12C long2 61
72 022499072 Tox 120 DNO. 191F-G6 2/22/99
TOX12C long2 62
73 022499073 Tox 120, RBL02229-250ppb-CC1, 2/ TOX12C long2 63
74 022499074 MeOH Blank
TOX12C long2 95
75 022499075 Tox 1 2 0 ,1 10F-G1-250ppb-MS, 2/22/ TOX12C long2 64
76 022499076 Tox 1 2 0 ,1 10F-G1-250ppb-MSD, 2/2 TOX12C long2 65
77 022499077 Tox 120, RBLQ2229-250ppb-CC2, 2/ TOX12C long2 e
78 022499078 MeOH Blank
TOX12C long2 95
79 022499079 Tox 120, RBL02229-H2O B lk-2,2/22 TOX12C long2 67
80 022499080 Tox 120, RBL02229-RBLiverB!k-1, 2 TOX12C long2 68
81 022499081 Tox 120, RBL02229-5ppb-2,2/22199 TOX12C long2 69
82 022499082 Tox 120, RBL02229-10ppb-2,2/22/9 TOX12C long2. 70
83 022499083 Tox 120, RBL02229-25ppt>2,2/22/9 TOX12C Iong2* 71
84 022499084 Tox 120, RBL02229-50ppb-2,2/22/9 TOX12C long2 72
8 5 022499085 Tox 120, RBL02229-100ppb-2,2/22/ TOX12C long2 73
86 022499086 Tox 120, RBL02229-250ppb-2,2/22/ TOX12C fong2 74
87 02249987 Tox 120, RBL02229-500ppb-2,2/22/ TOX12C long2 75
88 022499088 Tox 120, RBL02229-750ppb-2,2/22/ TOX12C long2 76
89 022499089 Tox 120, RBL02229-1000ppb-2,2/22 TOX12C long2 77
10 Analyte 10 QC ' 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte . 10 Analyte 10 Analyte 1 10 Analyte
10 Analyte 10 Analyte 10. Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard
10 Standard 10 Standard 10 Standard
000550
FACT: DATA REVIEW SUMMARY FORM
Date: c & f c f t f o
Analyst FACT StudyNumber
fD)C \ 2 0
Matrix
IZ Jru
Tune Points Dilutions?
[Jo
Analyte 1:
Pfc>
Acceptable LinearRange:
LOQ for this data set
Re-evaluatedfor differentrange?
A)
Analyte 2:
Acceptable Tintar Range: Clot
c *a 5 T ^ WfWCor^rs
LOO fcr this data set
` t --- ^
Re-evaluated for different range?
Analyte 3:
roAA
Acceptable LinearRnmw:
L O Q fix(fatadataset:
Re-evaluatedfor different range?
l\b
Analyte 4:
.513,
Acceptable Linear Rang e : /^ 0 ~~ / , <Dfaoppb>
LOQ fortius data set /. = o
Re-evaluated for.differentrange?
Analyte 5:
Acceptable Linear Rang e : /O -- ' ^ 5 0 pp3o
LOQ for this data set
b
Re-evaluatedfor diffiarentrange?
Analyte 6: Acceptable LinearRange: LOQ for this data set Re-evaluated for differentrange?
Analyte 7: Acceptable Linear Range: LOQ for tins data set Re-evaluated ft rent range?
000551
BESTCOPYAVMUBtE
Quantity Compound Summary Beport TGX-120 lUt Liver
Cample Lint: C: \MASSbTlGC\rACT-l.*no\SeleDl\032499a
Last modified: Wed Her 10 09:21:99 1999
Method:
C:\HA8SLraX\rACT-l.rx0\MetfeOa\O22499a
test modified: Tue Mar 09 13:93:43 1999
Job Code:
Printed:
Wed Mar 10 09:43:96 1999
>*ff1
Compound 1 roc
Mama
Saaplaliat Text
Type
Ctd Cone
XT
Area Si..
n a p e %Sev
1 022499001 *MeOH Blank
Analyte
7.991
131 1
0.00 MM
2 022499002 3 022499003
4022499004 9 022499009 6 022499006
7 022499007 t 022499009
KtfOB Blank Tox 120 K B L Q 2 2 2 9 -H 2 0 Blk-1, 2/22/99
Tox 120, XBL02229-RBLivarBik-l.2/22/99 T ox 120. *BL02229-Sppb-1, 2/22/99
Tox 120. RBL02229-10ppb-l. 2/22/99
T o x 120. *BL02229-2Sppb-l, 2/22/99 Tox 120. BBL02229-S0ppb-1, 2/22/99
Analyte Analyte
Analyte Standard
Standard
Standard Standard
5.99
12.00
29.90 59.90
7.941 .035`
7.960 7.992 7.991 7.986
91 418 961
642 1002 1612
' 0.00 MM 5.18 HI
28.41 MMX 14.74 MMX 30.18 MM
56.29 MM
374
231
-6
9 022499009 Tox 120. K3L02229-100ppb-l. 2/22/99 10 022499010 T ox 120. XBL02229-250ppb-l. 2/22/99 11 022499011 Tox 120. RBL02229-500ppb-l. 2/22/99 .
Standard Standard Standard
120.00 299.00 599.00
7.952 7.922 7.923
2898 7377
14301
111.40 MK 303.25 MM 599.79 MM
-071
1 2 0 2 2 4 9 9 0 1 2 Tox 120, RBL02229-750ppb-l, 2/22/99 13 022499013 Tox 120. *BL02229-1000ppb-l, 2/22/99
Standard Standard
S96.00 7.963. 1198.00 7.654
21841 27547
922.73 MM 1167.13 MM
-33
14 022499014 MeOH Blank
Analyte
15 022499015 Tox 120 DNO. 1457-C4, 2/22/99
Anelyte
7.954
3691
145.67 MM
16 022499016 Tox 120 DUO. 1467-G4, 2/22/99
Analyte
7.843
3012
116.29 MK
17 022499017 Tox 120 DNO. 1477-G4, 2/22/99
Analyte
7.852
2429
91.32 bb
II 022499018 Tox 120 DNO. 1487-G4. 2/22/99
Analyte
7.766 ; 3388
132.39 MM
19 022499019 Tox 120 DNO. 1497-G4, 2/22/99
Anelyte
20 022499020 Tox 120, BBL02229-250ppb-CCU, 2/22/99 QC
7.822 299.00 7.854
2735 6536
104.39 MK 267.21 bb
21 022499021 MeOH Blank * '
Anelyte
22 022499022 T o x 120 10. 1507-G4, 2/22/99
Analyte
7.958
2709
103.27 MM
23 022499023 Tox 120 DNO. 1S17-G4, 2/22/99
Anelyte
7.797
3855
152.38 MM
2402 2 4 9 9 0 2 4 Tox 120 DNO. 1527-G4, 2/22/99
Analyte
7.863
2880
110.60 MK
25 022499025 Tox 120 DNO. 1S37-G4, 2/22/99
Anelyte
7.859
2305
77.44 MK
26 022499026 Tox 120 DNO. 1S47-G4. 2 /2 2 /9 9
Anelyte
7.829
3459
135.41 MK
2 7 0 2 2 4 9 9 0 2 7 Tox 120, RBL02229-250ppb-CC12, 2/22/99 QC
299.00. 7.BS7
587S(
238.88 KM
28 022499028 MeOH Blank
Analyte
29 022499029 T a x 1 2 0 DNO. 1557-G4, 2/22/99 30 022499030 To* 120 DNO. 1577-G4, 2/22/99
Analyte Analyte
7.927 7.860
1975 4075
71.84 MM 161.80 MK
31 022499031 Tox 120 DNO. 158r>C4. 2 /2 2 /9 9
Anelyte
7.840
2570
97.34 MK
32 022499032 Tox 120 DNO. 1597-G4, 2/22/99
Anelyte
33 022499033 To x 120, RBL02229-250ppb-CClJ, 2 /2 2 / 9 9 Q C
7.856 299.00 7.569
4069 6615
161.56 MM 270.57 MK
34 022499034 MeOH Blank
Analyte
35 022499035 Tox 120 DNO. 1617-G5, 2/22/99
Analyte
7.926
2634
100.10 MM
36 022499036 Tox 120 DNO. 1627-G5, 2/22/99
Analyte
7.846
1826
65.48 KM
37 022499037 Tox 120 DNO. 1637-G5, 2/22/99
Analyte
7.858
2079
76.32 KM
38 022499038 Tox 120 DNO. 164r-GS, 2/22/99
Analyte
7.665
3031
117.09 bb
39 022499039 Tox 120 DNO. 1657-05, 2/22/99
Anelyte
7.868
2013
73.46 MM
40 022499040 Tox 120. RBLO2229-250ppb-CCU. 2/22/99 QC
299.00 .7.836
6827
279.66 MM
41 022499041 MeOH Blank
Analyte
42 022499042 TOx 120 DNO. 166r-GS, 2/22/99 43 022499043 Tox 120 DNO. 1677-05, 2/22/99
Analyte Analyte
7.937 7.869
2015 3075
73.56 MK 118.98 MK
44 022499044 Tox 120 DNO. 1687-05. 2/22/99
Anelyte
7.843
2398
89.96 IK
45 02249904S Tox 120 DNO. 1697-05, 2/22/99
Analyte
7.813
1835
65.85 MM
46 022499046 Tox 120 DNO. 1707-65 2/22/99
Analyte
7.858
1771
63.13 MK
47 022499047 Tox 120, RBL02229-250ppb-CC13, 2/22/99 Q C
299.00 7.838
7326
301.06 MK
48 022499048 MeOH Blank 49 022499049 Tox 120 DNO. 1717-65 2/22/99
Analyte Anelyte
7.947
1287
42.37 MM
50 022499050 Tox 120 DNO. 1737-05 2/22/99
Anelyte
7.825
1611
56.26 M K
51 022499051 Tox 120 DNO. 1747-65 2/22/99
Analyte
7.868
1421
48.15 MK
52 022499052 Tax 120 DNO. 1757-G5 2/22/99
Analyte
7.854
3019
116.58 MK
53 022499053 Tox 120 DNO. 1777-06 2/22/99
54 022499054 Tox 120, R8L02229-250ppb-CC16, 2/22/99
55 022499055. MeOH Bleak
'
56 022499056 Tox 120 pNQ. 1787-66 2/22/99
Anelyte
QC Anelyte
Analyte
299.00
7.750
7.861 7.869
7.921
2815804
8723 672
2500912
120593.. MM 360.87 MK
16.03 MK 107105.. MK
\21
57 022499057 Tox 120 DNO. 1797-06 2/22/99
Analyte
7.849
2888958
123726. MK
58 022499058 Tox 120 DNO. 1807-66 2/22/99
Anelyte
7.856 2768001
119402.. MK
59 022499059 60 022499060 61 022499061
62 022499062 63 022499063
64 022499064 65 02249906S 66 022499066 67 022499067 68 022499068 69 022499069 70 022499070
Tox 120 DNO. 1817-06 2/22/99 Tox 120 DNO. 1827-06 2/22/99 Tox 120, RBL02229-250ppb-CC17, 2/22/99
MeOH Blank Tox 120 DNO. 1837-06 2/22/99
Tox 120 DNO. .1847-66 2/22/99 Tox 120 DNO. 1957-06 2/22/99 Tox 120 DNO. 1867-G6 2/22/99 Tox 120 DNO. 1877-66 2/22/99
T o x 120, RBL02229-230ppb-CC16. 2/22/99 MeOH Blank T ox 120 DNO. 189P-66 2/22/99
Anelyte Analyte QC
Analyte Anelyte
Anelyte Anelyte Analyte Anelyte
QC Analyte Anelyte
299.00 299.00
7.752 7.576 7.854
7.927 7.931
7.853 7.653 7.849 7.861
7.861 7.931 7.937
3070609 2923061
10935 1317
2658942 2786810 2179533 2500338 2181490
9312 1129 1727B55
131506.. MK . 125187.'. M K
I,n 455.64 H K 1 43.65 MM 1 113874.. HK 1 119351.. MK 1 93340.52 MK 1 107081.. MK 1 93424.33 MK 1 386.12 MK 1 35.60 MK 1 73994.38 MK
52 29
71 022499071 Tox 120 DNO. 1907-66 2/22/99
Analyte
7.862
2127694
1 91120.17 KK
72 022499072 T ox 120 DNO. 1917-G6 2/22/99 73 022499073 Tex 120, BBL02229-250ppb-CCl, 2/22/99
Analyte QC
7.788 299.00 7.871
2156366 10156
2 92348.31 MK 1 422.36 MK
41
74 022499074 MeOH Blank
Analyte
7.867
1526
52.63 MK
75 022499075 Tox 120, 1107-Ol-250ppb-MS, 2/22/99
Analyte
7.696
13377
560.22 bb
76 022499076 T ox 120, 1107-61-250ppb-MSD, 2/22/99
Anelyte
7.782
15270
641.32 bb
77 022499077 T o x 120, HBL02229-250ppb-CC2, 2 /2 2 / 9 9
Analyte
76 022499078 MeOH Blank
Analyte
7.848 7.947
5786 418
235.07 KM 5.17 MM
79 022499079 TOX 120, BBL02229-K20 Blk-2, 2/22/99
Anelyte
7.918
366
3.01 bb
80 022499080 Tox 120, BBL02229-XSUverBlk-l. 2/22/99 Anelyte
81 022499081 Tox 120, RBLQ2229-5ppb-2, 2/22/99
Standard
7.990 5.99 7.881
416 603
5.10 bb 13.08 Mia
118
82 022499082
83 022499083 84 022499084 85 022499085 86 022499086 87 022499087
88 022499088
89 022499089
T o x 120, RBL02229-10ppb-2, 2/22/99
T ox 120,. B3L02229-25ppb-2, 2/22/99 Tox 120, JtBL02229-50ppb-2. 2/22/99 T ox 120, RBL02229-100ppb-2. 2/22/99
Tox 120, RBL02229-250ppb-2. 2/22/99 Tox 120, KBL02229-500ppb-2, 2/22/99
T ox 120, R8L02229-750ppb-2. 2/22/99
T ox 120, BBL02229-1000ppb-2, 2/22/99
Standard
12.00 7.912
Standard
29.90 7.884
Standard
59.90 7.899
Standard
120.00 7.852
Standard
299.00 7.869
Standard
599.00 7.868
Standard698.00 . 7.665
Standard
1198.00 7.866
636 1180 1924
3319
7281 13610 20328
29544
14.50 bbX 37.81 MK 69.67 MK
129.40 HK 399.12 MK 570.21 MM 857.94 MM 1252.66 KM
21
26 16
0
-5
-45
VvlDOO
Ytllbik
O* (lb
io*
U LM
S o u p 0 2 0 1 9 9 : Q u a n t i t a t e d o n 0 3 / 0 9 / 9 9 a n d p r i n t e d o n 0 3 / 1 0 / 9 9 b y DKB
000552
t
i!
3^
:* 1
*i
43 ^ * oS 3
o
33 S S S j i l i S S 5 3 5 S S i S
S3S25S3S33 33SSgS353
S8SSS5 r i ooo 5M
313 n-- o e
3
riH H H H H
f4 H H
H
3
3 53sssHs|=s SS55Ss Sas jj'
'533SS55S SaiS Sis s
3 SSSSSSSSS
s3
"ggssi i i
i
"5
-ts Rr
Ci?
s aaaaaaaaaaaaaaasssaa aaaaaa as
s aa3383s33s3sas3ssasa sssapi ss
IilSIs`!5 .....................\...i...i..y....i...-...m.... H i m s"a
H H H H H H H H rtH H H H H riH H H H flH
HrtrtrtrtH H H
a
s
3 p 5 | l | P | 5 3 5 1 | U s i l i II
a aaa
a asa
s i
H HHH
oo^hAWonH
O O <0 H I t H I H
HH I
II
It
aaaaaaaaa
sssssssss
"^'ssii
HHHHHHHHH
CO LO
LO O O O
l i S5S|i==
SsSSssSSssSsss SSSss 33
33s33
i Si S
3 S
3 Sss
s S
233333333
SS33SSSSS "sssass
3
Jt
?S3
!*?
Ifl
rS.43
jas
:S53 !sss
S
3
8
2
Si
Iririririririri-'
a sj*
!
m
SSi
s! iliil ii il il il1
ili! i
S S 5 3 S 3 3 3 'S 3 S S S 3 * 8 3 3 3 3 3 a 3S3^^^^
i!HI I s
iss:
l 1 i i i i l i i i i i i i i i i i i i i i i i i i i i i i i i i l i i i i i i i l i i i l i i i i i i i i i i i i i i i i i i i i i i i i i i i i i i i s i i i i i i i i i i i i i i -- 33333as333saaaassR5saaaasasaaa333333S533gsaasssRSss3S332sc3sga{:aapsa2asss3ssssss
TrT]
1
S
U
* oo-ooooooooooosoooooooooooggggogoog^ iiiiilliiiiiilil:
il:"s:ss
m
if
im iM m iffM M fK m m H H fm m fm m m im iM M fm E m m ifm ffK ffffH m iiii,
sggSSsrS
:5SgSSSSSSSSSS?&feSkgSiSSgSSSS!
& e r ^ s i i r i s i lr 8'ssii i m ^h i i i i i ^
r 8g i i i ! i ; i i i i i ; i i s i E ; i i i i i
m
I il UliII Iil i iilllfi!mm m m ss :ssS is i s i '
s
issi jfrr^l
isS
|ff ?!
l-t s5 (M%- MHI? M OA
*m ri ai
il! SSS CS 89 crsr
il
iI
SsssSssb,.
skgkkssks
% P
S5HSa
S s
4
sHs i
Ma eo M B
S. s
j
S
Mt1a0 oo
M1<00 oo
M00 . ao
M00 M1100 OO OO
M1100 OO
isSsSsKB. sssssssss
S
g I
J
*4 j
Ni <4
J
4<4<4<44444
WHH 4OM0 HHJ bu HHH HeH SSSsSsss S
Pir-*$r = m m -
s22`5
uwIM
0oOkB.
(MMM
HMI
01 H
0toail M4100
004B10
S iS lIE E r
!
r :*
p s;
1 11 1 11 1
IsssHss
m4HwMoAOOOMiuO*
HHH H H
/
SES g " S 8
BH J woM o10\ b
H OMJ wM
H MHM
M4
H
1MM0 b
HH
MM Ws
OO oM
H
IMSHI
HHHHHHHH
SsSsSbs. b s b c b :
O?
5
P
i l M 5ss
sss g - g
g
3 S
s
S 5
S
H M gg
mB
fC - f^c W1OI-IMMM1MIHHMO
s1 MM1 MUi 4wH1
Hf1l
HHi
MH we1
B1
H1HHfcWOMVO0i1
i
o c>
O
^ K?
O
r i- S
"* - - S
? &P -
ou> O :*So
'
loup 0301991 Quantitatad on 03/09/99 ani printad ob 03/10/99 by S M
|g ig ssg i3 sisessgss|eesesssssssssssseissssssgsss3ssssssss
:tt
g3ggr2igIf282fggIV -gffSV ffgK fgV V K fggf|fV V gffB ffgV fgggffV gIggH gfV gfgV H l
mmn lmBisisSkSs
*** m u m u 3 m i * m t*
ama
s SSSs s s k ,.
sssssssgs
s
s
s s
M
SSSSpSSB.. sSaaa
Bsasss
Sss S 5
H
M*4>
H(MB s
H
sa
*aCB .
mwH
SssSa
111111
HHH H
H
'r
H
H
H HH H
H
HHHHH
iSssHs
o o
WN(MMA>a^it>t
sm ss
o cn HA1MU1U1WM
cn
cn
SSS S skb b MB S
CBi
5
2
g s
G10 UO<n M1J0 w0 Ul b ggg
t) .a-
as tM b s b 0H1
MMO 001
g g
?.
g
09 HMi 110 0H11
sSss ssass
ggggg
(a*ABH19H0CHiB
P
S
g
11 a !
f
o O
Quantity Cenpousd S u a n r y A.port TCX-130 lUt Ll-nr
O l a Li.t: C i M O a s t a \ > i*CI-l..MO\lulaoS\oa3>a
tua aodifiad: Mad Kar 10 03:21:55 1 M
Mathod:
C:\HMSbnB\rjkCZ-l.MO\MatllOB\033493a
Laat nadifiad: Tua Max 0* U : 5 1 : U 191)
Bob Coda:
\
Tintad:
Mad Mar 10 09:43:30 1939
Ccnpouad Si K613
Mom '
t a p U U s f i Taxt
1 022499001 KaOH Blank
2 022499002 KaOH Blank
Typa Analyta
Analyta
Std Cena
ms
3 022499003 Tox 120, RBL02229-H20 Blk-1, 2/22/99
Analyta
4 022499004 S 022499003 6 02249900$ 7 022499007
8 022499008
9 022499009 10 022499010
1 1 022499011 12 022499012
13 022499013
T o x 120, XBU>2229~RBLivexftlk~l, 2/22/99
Tox 120, BBL02229-Sppb-1, 2/22/99
T o x 120, JtBL02229-10ppb-l. 2 / 2 2 / 9 9 To x 120, B8L02229-2Sppb-l. 2/22/99 Tox 120, RBL02229-50ppb-l, 2/22/99 Tox 120, *BL02229-100ppb-l, 2/22/99 To x 120, BBL02229-2S0ppb-l, 2/22/99
Tox 120, *BL02229-S00ppb-1, 2/22/99 Tox 120, BBL02229-7S0ppb-l, 2/22/99 *
T o x 120, XBL02229-1000ppb-l, 2 / 2 2 / 9 9
Analyta Standard Standard
Standard Standard Standard Standard Standard Standard Standard
5.99 12 .0 0
29.90 59.90 120.00
299.00 599.00 898.00 1198.00
9.373 9.374 9.398 9.380 9.345 9.347
9.289 9.280
] 14 033499014 15 033499013 1 033499010
KaOH Blank
Tox 130 OHO. 14SF-64, 3/33/99 Tox 120 DUO. 146,-64. 2/23/99
Aanlyt.
Bnalyta taalyta
17 022499017 Tox 130 OHO. 147V-04, 2/22/99
Analyta
18 022499018 Tox 130 OHO. 1489-04, 2/22/99
Analyta '
19 022499019 Tox 120 OHO. 1499-04, 3/22/99
Analyta
20 022499020 T o x 130, UL02229-250ppb-CCll, 2/23/99 QC
299.00 9.280
21 032499021 KaOH Blank
Analyta
32 022499023 Tox 110 OHO. 1509-04, 2/22/99
Analyta
21 022499023 Tox 120 DMO. 1319-04, 2/22/99
Analyta
24 022499024 Tox 120 DHO. 1S29-G4, 2/22/99
Analyta
23 022499025 Tox 120 SHO. 1339-04, 2/22/99
Analyta
28 022499026 Tox 120 DHO. 1549-04 , 2/22/99
Analyta
27 022499027 T o x 120, ABL02229-250ppb-CC12 , 2/23/99 QC
299.00 9.374
28 022499028 KaOH Blank
Analyta
29 022499029 T a x 120 UNO. 1559-04. 2/22/99
Analyta
30 022499030 Tox 120 DHO. 1579-04 , 2/22/99
Analyta
31 022499031 T o x 120 DHO. 1589-04, 2/22/99
Analyta
32 022499032 T o x 120 DHO. 1599-04, 2/22/99
Analyta
33 022499033 T o x 120, ABL02229-250pk-CC13, 2/22/99 QC
299.00 9.279
34 032499034 KaOH Blank
Analyta
33 022499035 T o x 120 DMO. 1519-05, 2/22/99
Analyta
35 022499038 T o x 120 DHO. 1529-05. 2/22/99
Analyta
37 022499037 Tox 120 DHO. 153r-05 , 2 /2 2 /9 9
Analyta
38 022499038 T o x 120 DHO. 184r-OS, 2/22/99
Analyta.
39 022499039 T o x 120 DHO. 1559-05, 2/22/99
Analyta
40 022499040 T o x 120, RBL02229-25Oppb-CCl4, 2/22/99 QC
299.00 9.262
41 022499041 KaOH Blank
Analyta
42 022499042 T o x 120' DHO. 1689-05, 2/22/99
Analyta
43 022499043 T a x 120 DHO. 1679-G5, 2/32/99
Analyta
44 022499044 Tox 120 DMO. 1689-05. 2/22/99
Analyta
45 022499045 Tox 120 DHO. 169r-OS. 2/22/99
Analyta
46 022499046 Tox 120 DHO. 1709-GS 2/22/99
Analyta
47 022499047 Tox 120. BBL02229-250ppb-CC15, 2/22/99 QC
399.00 9.262
48 022499048 KaOH Blank
Analyta
49 022499049 Tox 120 DHO. 1719-G5 2/22/99
Analyta
50 032499050 Tox 120 OHO. 1739-CS 2/22/99
Analyta
51 022499051 TOX 120 DHO. 1747-05 2/22/99
Analyta
J 32 022499052 53 022499053 54 022499054
TOX 120 DHO. 1757-65 3/22/99
Tox 120 DHO. 1777-G6 2/32/99 Tox 120, RaL02229-250ppfa-CC16. 2 /2 2 /9 9
Analyta
Analyta QC
299.00 9.287
55 022499055 MoOH Blank
Analyta
56 022499056 'Tox 120 DHO. 1787-66 2/22/99
Analyta
57 022499057 Tox 120 DHO. 1797-66 2/22/99
Analyta
58 022499058 Tox 120 DMO. 1807-66 2/22/99
Analyta
59 023499059 Tox 130 OHO. 1817-66 2/32/99
Analyta
60 022499060 Tox 120 DHO. 1627-66 2/22/99
Analyta
61 022499061 T o x 120, B8D02229-250ppb-CC17, 3/23/99 QC
299.00 9.277
62 022499062 KaOH Blank
Analyta
63 023499063 T ox 120 DHO. 1837-66 2/22/99
Analyta
64 022499064 Tox 120 DHO. 1847-66 2/22/99
Analyta
65 022499065 T ox 120 DHO. '1657-66 2/22/99
Analyta
66 022499066 T ox 120 DHO. 1667-06 2/22/99
Analyta
67 022499067 Tox 120 DMO. 1877-66 2/22/99
Analyta
68 022499068 T o x 120, Ut02239-3S0ppb-CC18, 2/22/99 QC
299.00 9.287
69 032499069 KaOH Blank
Analyta
70 022499070 Tox 120 DMO. 1697-66 2/32/99
Analyta
71 022499071 TO X 130 DHO. 1907-66 2/22/99
Analyta
72 022499072 T ox *120 DHO. 1917-66 2/22/99
Analyta
73 022499073 T o x 120., HBL02229-250ppb-CCl. 2/22/99 QC
299.00 9.289
74 022499074 KaOH Blank
Analyta
75 022499075 T o x 120, 110,-61-250ppb-MS. 2/22/99
Analyta
9.367
76 022499076 T o x 120, 110T-61-230ppb-KSD, 2/22/99
Analyta
9.295
77 022499077 T OX 120, MBL02229-250ppb-CC2, 2/33/99 Analyta
9.253
78 022499078 KaOH Blank
Analyta
79 022499079 T ox 120, R B U 2 2 2 9 - K 2 0 Blk-3, 2/22/99
Analyta
60 022499080 T o x 120. RBL02229-RBUvacBlk-l, 2/22/99 Analyta
81 022499081 T ox 120. MBL02229-5ppb-2, 2/22/99 82 022499082 T ox 120, RBLQ2229-10ppb-2, 2/22/99
Standard Standard
5.99 12.00
83 022499083- Tox 120, XBD02229-2Sppb-2, 2/22/99
Standard
29.90 9.297
84 022499084 T o x 120, HBL02229-50ppb-2, 2/22/99
Standard
59.90 9.311
85 022499085 T o x 120, BBb02229-100ppb-2, 2/22/99
Standard
120.00 9.278
66 022499086 T o x 120, MBL02229-250;b-3, 2/22/99 87 022499087 T o x 120, RAL02229-500ppb-2, 2/22/99
- Standard Standard
299.00 9.295 599.00 9.294
aa 022499088 To x 120, RBl02223-750ppb-2. 2/22/99
Standard
898.00 9.293
89 022499089 T ox 120. RBL02229-1000ppb-2, 2/22/99
Standard
1198.00 9.294
lo u p 020109: Q u u t l t i t s d p a 0 3 /0 9 /9 9 pad p r in te d o a 0 3 /1 0 /9 9 b y drb
I
A n a Di
Cons. lo Mo o t
44 89 148 281
$10
116$ 1788 2127
1 8.41 MM 1 32.10 bb 1 63.32 MM 1 133.38 bb 1 307.64 bb . 1 602.07 bbX 1 930.80 bbX 1 1110.50 bbX
-30 7
6
11 3 1 4
-7
460 1 239.14 bb
459 1 227.79 bb 561 1 282.01 bb
571 1 287.06 bb
634 . 1 320.22 bb
584 1 293.99 b b
578 1 290.74 bb
489 1 243.59 bb 307 1 253.42 bb 446 1 220.79 MM 466 1 231.31 MM
62 142
260 566
1158 1610
2343
1 17.83 MM 1 59.88 MX
1 122.65 MX 1 284.38 MM
1 397.74 bbX 1 836.67 MMX
1 1224.94 MMX
0-40 2
-S0
-7 2
00055S
S* 3
O')]
guaaetfy Cnpoiusd tv m a a x y a p o n TOX-13Q lut Livar
Sanpla Piati C:\WUaL-nJX\7ACT-l.ano\IanBlaDB\02219S*
La. laodlfladi N ad Mac 10 OSiSXtSS 1191
Katbod:
Ct\MXSSLmX\nCT-l.MO\MatllBB\0224m
Laat aodlCladi Tua Har OS 1 3 : 3 1 : 0 U S 9
Job Codai
Sx lat ad
' M a d Mar 10 0 9 i O i 5 1S9S
COoponad St If
1 Mum
Saxplaliat Text
Typa
Std Cone
1 022499001 KcOH Blank
Analyta
2 022499002 MaOH Blank
Analyta
2 022499003 T o x 120 BBL02229-X2O Blk-1. 2/22/99
Analyte
4 022499004 T O x 120 ftBL02229-RBLiverBlk-l, 2/22/99 Analyta
S 022499005 Tox 120 XBL02229-5ppb-l 2/22/99 6 022499006 T ox 120 RB102229-10ppb-l, 2/22/99
Standard Standard
494.00 494.00
7 022499007 9 022499008 9 022499009 10 022499010
Tox 120 KBt02229-2Sppb-l, 2/22/99 Tox 120 BBL02229-50ppb-l, 2/22/99 Tox 120 XBL02229-100ppb-l, 2/22/99 T o x 120 BBL02229-250ppb-l, 2/22/99
Standard Standard Standard Standard
494.00 494.00 494.00
494.00
11 022499011 Tox 120,BBL02229-500ppb-l, 2/22/99 12 022499012 Tox 120 HBL02229-750ppb-l, 2/22/95
Standard Standard
494.00 494.00
13 022499013 To x 120 BBL02229-1000ppb-l, 2/22/99
Standard
494.00
14 022499014 MaOH Blank
Analyta
IS 022499015 T O X 120 DNO. 1457-434, 2/22/99
Analyta
16 022499016 T o x 120 DUO. 1467-04 2/22/99
Analyta
17 022499017 TOX 120 DNO. 1477-64, 2/22/99
Analyta
18 022499018 Tox 120 DNO. 1467-G4, 2/22/99
Analyte
19 022499019 Tox 120 DNO. 1497-04. 2/22/99 20 022499020 To x 120, U L 0 2 2 2 9 - 2 5 0 p p b - C C U # 2/22/99
Analyta QC
494.00
21 022499021 MaOH Blank
Analyta
22 022499022 T O X 120 DMO. 1907-04, 2/22/99
Analyta
23 022499023 TOX 120 DNO. 1517-04, 2/22/99
Analyta
24 022499024 Tox 120 ONO. 1527-C4, 2/22/99
Analyta
25 02249902S Tox 120 DNO. 1537-G4 2/22/99
Analyta
26 022499026 Tox 120 DNO. 1547-64, 2/22/99
Analyta
27 022499027 Tox 120, BBL02229-259ppb-GC12, 2/22/99 oc
494.00
26 022499028 MaOH Blank
Analyta
29 022499029 T o x 120 ONO. 1557-C4, 2/22/99
Analyta
30 022499030 T9X 120 DNO. 1577-64, 2/22/99
Analyta
31 022499031 Tox 120 DNO. 1587-64, 2/22/99
Analyta
32 022499032 Tox 120 DNO. 1597-64. 2/22/99
Analyta
33 022499033 T ox 120, kBL02229-250ppb-CC13, 2/22/99 QC
494.00
34 022499034 MaOH Blank
Analyta
35 022499035 T O X 120 DNO. 1617-05, 2/22/99
Analyta
36 022499036 Tox 120 ONO. 1627-QS, 2/22/99
Analyta
37 022499037 Tox 120 DNO. 1637-65, 2/22/99
Analyta
38 022499038 T ox 120 DNO. 1647-05, 2/22/99
Analyta
39 022499039 Tox 120 DNO. 165r-OS, 2/22/99
Analyta
40 022499040 T o x 120, HBL02229-250ppb-CC14, 2/22/99 QC
494.00
41 022499041 MaOH Blank
Analyte
42 022499042 Tox 120 DNO. 1667-05, 2/22/99
Analyte
43 022499043 Tox 120 DNO. 1677-05, 2/22/99
Analyta
44 022499044 Tox 120 DNO. 1687-05. 2/22/99
Analyta
45 022499015 Tox 120 DNO. 1697-05, 2/22/99
Analyta .
46 022499046 T ox 120 DNO. 1707-05 2/22/99
Analyta
47 022499047 T o x 120, XBL02229-2S0ppb-CC15, 2/22/99 QC
`494.00
48 022499048 MaOH Blank
Analyta
49 022499049 Tox 120 DNO. 1717-GS 2/22/99
Analyta
SO 022499050 Tox 120 DNO. 1737-05 2/22/99
Analyta
SI 022499051 T o x 120 DNO. 1747-05 2 /2 2 /9 9
Analyta
S2 022499052 T o x 120 DNO. 1757-05 2/22/99
Analyte
S3 022499053 Tox 120 DNO. 1777-06 2/22/99
Analyta
54 022499054 Tox 120, BBL02229-250ppb-CC16. 2/22/99 QC
494.00
55 022499055 MaOH Blank
Analyta
56 022499056* T ox 120 DNO. 1787-06 2/22/99
Analyta
57 022499057 T o x 120 TOO. 1797-06 2/22/99
Analyta
58 022499058 Tox 120 DNO. 1807-08 2/22/99
Analyta
59 023499059 Tox 120 DNO* 1817-06 2/22/99
Analyta
60 022499060 T o x 120 DNO. 1827-66 2/22/99
Analyta
61 022499061 T ox 120, KBL02229-250ppb-CC17, 2/22/99 QC
494.00
62 022499062 MaOH Blank
Analyta
63 022499063 T O x 120 DNO. 1837-06 2 / 2 2 / 9 9
Analyta
64 022499064 T o x 120 ONO. 1847-66 2/22/99
Analyta
65 022499065 TOX 120 DNO.*1857-06 2/22/99
Analyta
66 022499066 T ox 120 DNO. 186r-G6 2/22/99
Analyta
67 022499067 T ox 12 DNO. 1677-06 2/22/99
Analyta
68 022499068 T o x 120, R8Cs02229-250ppb-CC19. 2 / 2 2 / 9 9 QC
494.00
69 022499069 MaOH Blank
Analyta
70 022499070 TOX 120 DNO. 1897-66 2/22/99
Analyta
71 022499071 T ox 120 ONO. 190P-G6 2/22/99
Analyta
72 022499072 T o N 120 DNO. 1917-66 2/22/99
Analyta
73 022499073 T ox 120, ftBlA2229-250ppb-CCl. 2/22/99
QC
494.00
74 022499074 MaOH Blank
Analyta
75 02249907S T O X 120. 1107-61-250ppb-MS, 2 / 2 2 / 9 9
Analyta
76 022499076 T ox 120, 1107-Gl-250ppb-MSD, 2/22/99
Analyta
77 022499077 Tox 120, BBL02229-250ppb-CC2. 2/22/99
Analyta
78 022499078 MaOH Blank
Analyta
79 022499079 T ox 120, R8L02229-H2O Blk-2, 2/22/99
Analyta
90 022499080 T ox 120, ftBL02229-RBLiverBlk-l. 2/22/99 Analyta
91 022499081 T ox 120, RBL02229-5ppb-2, 2/22/99
Standard
494.00
82 022499082 T ox 120, RBL02229-10ppb-2, 2/22/99
Standard
494.00
83 022499083 T ox 120, *BL02229-25ppb-2, 2/22/99
Standard
494.00
84 022499084 Tox 120, HBL02229-50ppb-2, 2/22/99
Standard
494.00
65 02249908S T OX 120, KBL02229-100ppb-2, 2/22/99
Standard
494.00
86 022499086 T ox 120, BBt02229-250ppb-2, 2/22/99
Standard
494.00
87 022499087 T ox 120, RBL02229-500ppb-2, 2/22/99
Standard
494.00
88 022499088 T o x 120, RBL02229-750ppb-2, 2/22/99
Standard
494.00
89 022499089 T ox 120, RBt02229-1000ppb-2, 2/22/99
Standard
494.00
Jtt
7.314 7.320 7.252 7.236 7.235 7.194 7.237 7.209 7.122 7.145 7.13S
7.237 7.136 7.050 7.049 7.116 7.138
7.163 7.082 7.062 7.142 7.031 7.060
7.212 7.142 7.130 7.054 7.072
7.209 7.131 7.144 7.063 7.160 7.119
7.223 7.068 7.130 7.106 7.057 7.124
7.231 7.112 7.151 7.138 7.035 7.145
7.211 7.134 7.059 7.037 7.035 7.141
7.131 7.142 7.137 7.135 7.143 7.145
7.137 7.068 7.080 6.996
7.152 7.066 7.050
7.208 7.197 7.169 7.122 7.093 7.105 7.136 7.068 7.066 7.064 7.064
Araa Dl Cone. Tlaga kDev
348 278 665 847 768 713 583 663 675 673 746
389 632 421 472 617 672
329 481 599 560 402 555
316 473 464 362 654
383 676 588 604 515 558
349 497 586 441 353 542
340 534 513 493 231 SIS
236 216 226 235 204 571
199 225
281 206 308 575
285 351 306 546
322 366 531
308 316 70 704 756 748 691 732 642 638 537
1 248.61 MM 1 198.55 bfa 1 474.76 MK 1 604.74 MK 1 548.31 bb 2 509.24 bb 1 416.56 bfa 1 473.22 bfa 1 482.12 MM 1 480.59 bb 1 532.80 bb
-50 -60
-4 22
11 3
-16 -4 -2
-3 8
1 278.17 MM 1 451.67 bb 1 300.56 bb 1 336.95 bb 1 441.04 bb 1 480.18 bb
-44 -9
-39 -32 -11
-3
1 235.15 bb
-52
1 343.87 bb - .30
1 428.10 bb
.-13
1 414.05 M K
r 287.31 bb
-16 -42
i 396.73 bb
-20
i 225.86 MM i 337.66 MM i 331.42 MM
2 258.72 bb 1 466.87 bb
-54 -32
-33
-48 -5
1 273.25 MK 1 482.50 bb .1 420.31 bb
1 431.38 bb 1 367.61 bb 1 398.50 bb
-45 -2
-15
-13 -26 -19
1 249.01 bb 1 355.24 bb
1 418.91 MM 1 314.98 bb 1 252.29 MX
1 387.24 MM
-50
-28
-15 -36
-49 -22
1 242.98 MM 1 381.17 fab
1 366.66 MM
1 352.10 MK 1 164;71 MX 1 367.80 bb
-51
-23 -26 -29
-- 67 -26
1 168.62 bb 1 154.58 bb 1 161.28 M X 1 167.67 fab 1 146.02 bb
K 407.90 bb
i 142.43 bb i 1643.54 b b i 200.47 MK i 148.91 bb i 220.14 bb i 410.85 bb
-66 -69 -- 67 -66 -70 -17
-71 -68 -59 -70 -55 -17
i 203.31 bb i 250.92 MK i 218.67 bfa i 389.67 bb i 229.80 bb i 261.34 bb i 379.16 bb i 219.86 MM i 226.93 XX
i 478.13 XX i 902.64 bb i 940.16 bb i 533.98 bb i 493.58 bb i 523.07 MM i 458.22 bb i 455.91 bb i 313.25 MM
-59 -49 -56 -21
-S3 -47 -23
-55 -54
-3 2 9 8 0 6
-7 --8 22
Soup 020ISS i Q u a n tlta ta d o a 0 1/0S /S S an d p d a t a d o a 0 3 /1 0 /9 9 b y m
000557
Vasa
Q u a n tify C a lib ra tio n te p o rt TCX-120 a t l i r a *
Calibration: C i M I U I U I B \ n c i - t . n o \ c u n n M I ] U a
Laat laodifiad: M a d Max 10 0 9 : : 03 1999
rintad:
Mad Max 10 00:44:01 1000
*aga X
lo u p 030100: Q u a n tita ta d o n 0 3 /0 0 /0 0 an d p x la ta d on 0 3 /1 0 /0 0 b y su a
000558
Oua n e l i y C a l i b r a t i o n B a p o rt TOX- 1 2 0 l u e U v a r
calibration c>\MaMian\ric>-l.PM\curraBS\0324*a
taat nodifladi ad Mar 10 Mi43iOS I J
Paint ads
W a d Mar IS OS U i 01 ISSS
c u p 0 2 0 1 S S I Q o a a t l t a t a d o n O S /O S /S S a n d p r l n t a d o n 0 3 / 1 0 / S S t r y UHM
000559
Quaatlfy Calibrati apere tax-iao aae U t u
Calibrati: c:\nanuicc\racf-l.no\CaraaDa\0331Ma
Late acdiflad: ad Kar 10 03:43:03 ISO*
relatad:
ad Mar 10 03:44:01 1333
Vaga 3
lo u p 030133: Q u aaeltaead o a 0 3 /0 3 /3 3 a a d p r la ta d o a 0 3 /1 0 /3 3 far S ta
000560
Quantify c alibration Jtapoxe OX-120 xae b i-m r
Calibration! Ci \ M M M N X \ n - l .I*O\CuraaIJ\03241a
M a t aodifiad: M Max 10 01:43:0s loss
yrintad:
a d Max 10 Ofi44i01 Ifff
)
*0 4
*oup 0301591 Q u a n tita ta d e n 0 3 /0 1 /1 an d p r in ta d a n 0 1 /1 0 /9 1 b y SM
000561
Q u a n tity C a lib ra tio n to s e n TC X -120 afe U w a r
Calibration: Cs\NU*Z<l\ncS<l.ntO\cuzvan\>33Mla
beat aoditladi tod Mar IS 05]43:OS 1S5J
rintad:
ad Mar 1 05:44:01 1
Soup 0 2 0 1 9 h Cuanti catad o n 0 3 / 0 9 / 9 9 aad printed e n 0 3 / 1 0 / 9 9 D y D U
000562
Quantify Calibration M p o r t W3X-120 tat Litar
Calibration! C:\XUlLrax\racT-l.nO\CurvaBa\022199a
Last nodlflad) Wad H u 10 09>t2:OS ISOS
Tintad!
Wad War 10 Ots U i O l 1
lo u p 0201991 Q u a n tita ta d on 0 3 /0 9 /9 9 an d p r ln ta d o n 0 3 /1 0 /9 9 b y r a t
000563
Mmtbcd p e rt
Hathed f i l a t Laat aed lfiad i
Coapoundt
Ci \XASSLYIfXNTXCT-l.rRO\KathDB\022499a.ia31J f r i Pab 3 1 0 i3 0 il9 139
li PPOS
P r in t< d l____________ t u . K r 0 1 3 .3 3 ,0 1 1993
Compound S a M t Internal Sf>i Quantify Trace* Cone, of Standards*
Vsar RP Valttai Daar Peak Paccori Xnt.gr.t. Window Pacteri
PPOS (Nona) TIC Cone. A
1.000000, Disabled 1.000000
a.ooo
Ratancion Tina (mina) i Ralatlva R.caution Tiaai Tint. Window (min.) i
Task Saloctiom
1.03, tnablad 0.000. Diaablad 1.030
Dearest
Aeaponse Types Responses
Ixtexnal Areas
Polynomial Type*
Point of Origin* Pit Weightings Axis Transformation*
Linear Bxeliade
XOne
Dnltai
Peak-to-peak amplitudes
iss
Smoothing* Window size (scans)* Number of smooths*
Smoothing methods
Disabled 1
2
Join valley threshold* Seduce peek tailings
Seize baseline maximum*
30.00 70.00 4.00
Draw vertical threshold*
Detect shoulderst Shoulder threshold*
10.00 Diublad 10.00
Relative heights Absolute heights Relative areas. Absolute area*
30.00, Enabled , 0.00p Disabled
3.00* Inabled
.0.00. Disabled
fagt j
S o U p 0 3 .0 1 ^
L A C 0 3 _________________________________
# .!
.
^
'
*
000S64
M ethod R e p o rt
M a thod filai Laat modified!
Compound!
printed!
C: \MASSLYNX\rRCT-l.PRO\M.thEB\02H 9 9 a . m d b Tri T ab 26 10i3 0 i 39 1999
2i ?ras
.
Tua M a r 09 1 1 i2 2 i01 1999
Compound Nanai I n t a n a i Ra. 1 Quantify Tracal Cone, of Standards!
Usar HP Valuei Usar Peak Paetori Integrata Window Pactor:
WHS (Nna] TIC Cone. A
1.000000, Disabled 1.000000 2.000
Retention Tine (alna) t Relative Retention Tine Tine Window (nina) i
Peak Selectioni
.363, enabled 0.000, Disabled 1.117
Keenest
Response types Responses
Internal Arena
Polynomial types Point of Origins Pit Weightings Axis TransCessation
Linear Seclude Bone
Bone
Quitss
Peak-to-paak aaplftude*
Ih j i Window size (scans)i Dumber of smoothsi Smoothing method!
115
Disabled 1 2
Join valley threshold! * Reduce peak tailing Raise baseline maximum
30.00
70.00 4.00
Draw vertical thresholds Detect shoulders
Shoulder thresholds
10.00
Disabled 10.00
Relative height! Absolute heights Relative areas
Absolute areas
50.00 Knabled a 0 .00, Disabled
3.00, Knabled 0.00, Disabled
Vaga 2
Soup m onv LAC n i l i l w
000565
M ethod R e p o rt Method f l l e i te a t M odified!
Compound:
Printed!
C !\W A SSl\rA CT-l.PR 0\H ethI\022499e.edli
P r l Teh 2S 10 00, 1 1999
3 *0** Tue Her OS 13i22i01 1999
Compound N u t t Internal M t . i Quantify Traeai. Cone, of Standardai
Dear XT Value i Deer Teak Taetori Integrate Window Factor!
nu . (Hone)
TIC Cone. A
1 .000000, Diaahled 1.000000 2.000
Retention Time (mins) * Xelative Retention Timei Tin* Kladov (mine)
Teak Selection!
7.490, Xneblad 0.000, Disabled 0.902
Meareat
Xaeponae Typei lspout
Polynomial Typei Point of Origin! Tit Weightingi Axle Trane formation i
Xru
elude gpim mime
Units*
Peak-to-peak aajplltudat
185
Smoothing* Window ni*o (scans)i
number of smooths Smoothing method!
. Disabled
1 2
Join valley threshold!' Reduce peak tailing! Raise baseline marine!
30.00 70.00 4.00
Drew vertical threshold Detect shoulders
Shoulder threshold
10.00
Oiaabled 10.00
Xelative height Absolute height
Xelative area* Absolute areas
50.00, Enabled , 0.00, Disabled
3.00 Enabled 0 .00 Disabled
Soup dZqiM
IAC 031Oik1
000566
Kathod f ll i L u c aodilled!
Compound!
Printed!
C t\]U SS Z ,m rA C T -l.PliO U lehO a\023199a-*<U >. Fcl r.b 2 IO1IO1 1999
11 HSU
Tut Mar 09 13i22OX 1999
Compound Hanoi Internal Ref.i Quantify Tracei Cone, of Standardes
User AT Valuot User Peak Pactori Integrate Window Pactori
X913 (HOno] TIC Cone A
1 .000000, Diaablod 1.000000 3.000
Retention T ine (nine) Relative Retention Tiaoi Tine Window (nine) i
Peak Selection!
B.S4S, Enabled 0.000, Disabled 0.730
nearest
Response Typei Response!
External Aroan
Polynomial Typei Point of Oriffin i
Pit Weighting! Axis Transformation'
Linear Exclude
Wona Kona
Unite2
Peak-to-peak amplitude! *
IBS
Snoothinffi Window sire (scans) x
unbar of smoothai Smoothing method i
Diaablad -1
2
Join valley threshold! Reduce peak tailingi Raise baseline suximusu
30.00 70.00 .4.00
Draw vertical threshold! Detect shoulders!
Shoulder threshold!
10.00 Disabled
10.00
Relative height! Absolute height! Relative area.t
Absolute areat
.
50.00, enabled , 0.00, Disabled
3.00, Rnabled
. 0.00, Disabled
Soup 020m
LAC &blo4v\ j? .
000567
Method Repart
Method filai Lest modified!
Compound:
printedi
Ci \MASSLYNX\rACT-l. PHO\HsthDB\03J499e.mdb fri Tab It 10i30t39 199)
Si MS 13 Tue Her 09 13i33i01 1999
Compound Marne Internal Ref.i Quantify Trace! Cone, of standardet
Osar RP Value t User peek Vectors Integrate Window Vectori
Mill (Monel TIC Cone. A
1.000000, Disabled 1.000000 3.000
Retention Time (mins) i Relative Retention Timet Time Window (mine) t
Peak Selectiont
9.591, Rnefaled 0.000, Disabled 0.730
Nearest
Response Type MSpOOMi
Polynomial types Point of Origins pit Weightingi >xie Tramafexmatioat
^eternal Areas
Trl BNCluda Rone Rone
tJfcitsi
Peafc-to-paafc anplitudst
Smoothing! Window aixa (scene) t Number of smooths! Smoothing methods
Join valley threshold! ' Reduce peek tailings Raise baseline mexinumi
Draw vertical thrasholdi Datact shoulders* Shoulder thresholds
Relative height! Absolute height! Relative areas. Absoluta areas
1RS*
Disabled 1 3
30.00 70.00 4.00
10.00 Disabled 10.00
30.00, enabled 0.00, Disabled 3.00, Rnebled 0.00. Disabled
5duo oaom
1
'
'*
.
i a c ciIm Iw
000568
M ethod p o rt
Method idle i Last nodifieds
Compound t
printedi
C. \MASSL7HX\fACT-l. PRO\HethDB\023499a.sdb Pri eh It 10 s10-.19 1999
Si IS
Tam H er 9 1 1:23.01 1999
Cenpound Hanses I n t e m e l Ref.s Quantity Trace> Cone, of Standardas
Dear U Valuei Uaer Peak Pactozi Integrate Window factors
13 [Hone] TIC Coae. a
1.000900, Disabled 1.000000 2.000
Retention Time (mine) Relative Retention Times Tine Window (mine] s
Peak Selections
7.410 enabled 0.000, Diaabled 0.990
Heareat
Response Types
Polynomial Types
Point ot origins Pit Weightings Riaie Transformations
Unear Kssclude Nona
Unites Peak-to-peak asssplitssdes
Smoothing s
Window size (cans!s
Humber of asnoothas Ssssoothing methods
Diaabled .1
3
join alloy threshold: Radsice peak tailings Raima baseline manimums
30.01 70.01 4.00
D raw vesrtical thresholds Detect shoulderss Shoulder threshold:
Relative heights Absolute heights Relative areas Absolute areas
10.00 Disabled 10.00
50.00, Haablad 0.00, Disabled 3.00, Snabled 0.00, Disabled
SpUj) 0%cm U c Qilo'ilw
000569
M ADELINE 041098: FACT E quipm ent Log 98053 U A
PAGE
I Q uattro II, #6443E
1 DATE: 03/01/99 ANALYST: drb STUDY NUM BER TOX 120 M ATRIX: ra t liver DESCRIPTION: liver extr. dilutions
] FACT M ethod: _
Injection volume, 10 uL
Cycle tim e: 16 m in. Electrode: Z-Spray_______
1 MobUe Phase:
(AM.O mM NH4CH3CQ2 . (B) MeOH_____________
TN-A- o r stadrd num ber (A) 99011-1-9_________
QB) TN-A-2384
] Prepared (date/by): ` ' ( A) 2/12/99 m ee_________ " (B) 2/20/99 dib
E x p ires:
(A) 08/12/99___________
(B) 8/18/99_____________
] Store at room tem p? (Yj o r N
O th er_________________________
T G uard colum n: None________________ .__
P art number: na ________
L ot num ber: na_________________________
I A nalytical colum n: keystone betasil__________
i
Size: 2 x 100mm__________ .
T y p e:c l8 __________ Sphere size: Sum _____________
i
t
P art num ber: 105-701-2_______ L ot number: p8f!3
Serial number 118739a
]
j A nalytical param eters:
D rying gas (L/hr): 300
ESI nebulizing'gas (L/hr) 15
Sheath gas (L/hr) 0
-
J LC Flow rate: 300 uL/m in. B ack pressure: 120 bar
3 H PL C solvent g rad ien t: long2ex
J Time (min.-) 1) 0.00
%B 40
2) 8.5
90
I 3 1.1.0 4 )iy_
51 12.5
I 6) 13.00
90 100 100 40
>
. '
] 000570
1
Tuning Method Report
Method:
C :\MASSLYNX\RATS. PRO\ACQUDB\PFOS
Printed:
Mon M a r 01 17:17:40 1999
. . M W _____ ( A L o r / i o l v
Page 1
BESTCOPYAVAILABLE
MS
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 45 5
0.2 150 250
3.00 -46 -4
153 251
Pressures
Analyser Vacuum Gas Cell
Rdbk
6.4e-5 5.3e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
Set
15.0 15.0 2.0 ? 0.0 ' 5 650
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rd
15.0
15.0 20
2.0 0.0 40
0 650
20
41 18
000571
S c a n n in g MaChod R e p o rt
H athod: l a s t M odiriad:
P rinted:
C:\HhS9LYNX\RhTS.PR0\ACQUDB\TOX12QA
Mon M ar 01 1 7 :1 6 :3 1990
Mon M ar 01 1 7 :1 7 :5 1 1999
,
n iJ jJ iu
S olvant B alay ( alna > i
0 .0 0
<s>
rtM to u ..
LAC
runetion t 1
MM o ( 3 Mata P a i r s ESP- )
In ta r Channal Balay ( Saca ) i Span ( D altona ) t
S t a r t T la a ( Mina ) : End T laa t Mina ) i
Rapaata i Channal P arant D aughtar
o .o a 0.00 0.00 13.50
1
D w sll (s.c a ) C e l l E n erg y <eV)
Cons
1
399.00 80.00
0 .2 5
2
399.00 99.00
0.25
3 399.00 130.00 0.25
45 45 45
0 0
60
j
]
r
:
1
runetion S 3
MM o f 3 Maaa P a i r s ESP- 1
In ta r Channal Balay ( Saca ) i Span ( S altona ) : S t a r t T la a ( Mina ) t End T laa < Mina ) i Rapaata i
Channal P arant O aughtar
0 .0 3 0 .0 0 0 .0 0
13.50
1
D w sll
(Sees)
' c o li Energy
(aT)
1
499.00 90.00
0 .2 5
2
499.00 99.00
0 .2 5
3 499.00 130.00 0.25
45 45 ' 45
Cons Cv |
60 60 60
Ponction : 3
MRM o f 3 Maaa P a i r s C ESP- )
In ta r Channal Balay ( Saca ) i
Span ( D altona ) i
S t a r t T laa ( Mina I I
End T laa t Mina ) t
Rapaata :
Channal P aran t Daughtar
0.03 0 .0 0 0 .0 0 13.50
1 D w sll
ISaca)
C oli Energy
taV)
Cons
1 299.00 90.00 . 0.25
2
299.00 . 99.00
0.25
45 45
35 35
runetion : 4
MRM o f 3 M u s P a ir a < ESP- >
I n ta r Channel Dalay ( Seen ) : Span ( D altone ) S t a r t T la a ( MIna ) 1 End T laa ( Hin ) t R apaata :
Channal P arant Daughtar
0 .0 3 0 .0 0 0 .0 0 13.50
l
D w sll {Saca) C o ll E nergy (eV)
1 413.00 119.00 0.25 2 413.00 199.00 0.25 3 413.00 219.00 0.25
30 20 30
Cons ( V >
15 15 15
J runetion < S
MRM of 3 Maaa Paira ( ESP- I
Zntsr Chsansl Dslsy ( Saca ) i 0.03
Span < Osltons ) t
0.00
Stsrt Tims ( M i m ) :
t 0.00
End Tims ( Hins ) : Rspsats i
13.50
1
Channal Parent Daughtar Dwsll (Sees) Coli Energy (sV) Cone ( V )
1
460.00 189.00
0.25
2
469.00 219.00
0.25
3
469.00 119.00
0.25
45 * 45 45
30 30
30
] Punetlon l S
MRM o f 3 Maas P a irs < ESP- )
In te r Channel D alay { Saca ) t 0.03
opan { D sltons ) i
0.00
S t a r t Tims < Hins ) t
0 .0 0
nd Tims ( H ins ) :
13.50
R spsats : Channel Parent
D au g h tar
1 D w sll IS .e s ) C o l l E nergy (aV)
Cons ( v )
1 569.00 169.00 0.25 2 569.00 219.00 0.25 3 569.00 119.00 0.25
45
45 45
35
35 35
J
P u n e tlo n i T MRM o f 1 K u a P a i r ( ESP- )
In ta r Channal Balay ( Saca ) : Span { S altona ) i S t a r t T in e | Mina ) < End T ina ( Mina ) i
Rapaata > Channal P aran t Daughtar
0 .0 3 0.00 0 .0 0
13.50
1 Dwall . (Saea)
C oll
Enargy
laV)
Cons { v )
1
427.00 0 .0 0
0 .2 5
40
40
}
Pags l
000572
M asslynx Sample List
Sample List: C:\M ASSLYNX\rats.PRO\Sam pleDB\030199c.SPL Printed: Mon Mar 0117:17:14 1999
Pagel
File Name File T ext
1 030199001 MeOH Blank 2 030199002 MeOH Blank
3 030199003 Tox 120, RBL02229-H2O B lk -1 ,2/22/99 4 030199004 Tox 120, RBL02229-RBLIverBlk-1,2 /2 2 /9 9 5 030199005 Tox 120, RBL02229-5ppb-1,2 /2 2 /9 9
6 030199006 Tox 120, R B L 02229-10p p H , 2/22/99
7 030199007 Tox 120, RBL02229-25ppb-1,2 /2 2 /9 9
8 030199008 Tox 120, RBL02229-50ppb-1,2/22/99
9 030199009 Tox 120, RBL02229-100ppb-1,2 /2 2 /9 9 '
10 030199010 Tox 120, R BL02229-250ppb-1,2/22/99 11 030199011 Tox 120, RBL02229-500ppb-1,2 /2 2 /9 9 12 030199012 Tox 120, RBL02229-750ppb-1,2/22/99
13 030199013 Tox 120, RBL02229-1000ppb-1,2 /2 2 /9 9 14 030199014 MeOH Blank 15 030199015" Tox 120 DNO. 1 2 5 F -G 2 ,2/22/99 D 5 16 030199016 Tox 120 DNO. 126F-G 2, 2/22/99 D 5 17 030199017 Tox 120 DNO. 127F-G2, 2/22/99 D5 18 030199018 Tox 120 DNO. 1 2 9F -G 3,2/22/99 D2 19 030199019 Tox 120 DNO. 130 F -G 3 ,2/22/99 D2
20 030199020 Tox 120, RBL02229-250ppb-CC1,2 /2 2 /9 9 21 030199021 MeOH Blank 22 030199022 Tox 120 DNO. 131F-G3, 2/22/99 D2
23 030199023 Tox 120 DNO. 132F-G3, 2/22/99 DZ 24 030199024 Tox 120 DNO. 133F-G 3, 2/22/99 D2 25 030199025 Tox 120 DNO. 1 3 4F -G 3,2/22/99 D2 26 030199026 Tox 120 DNO. 135F-G3, 2/22/99 D2 27 030199027 Tox 120, R BL02229-250ppb-CC2,2/22/99 28 030199028 MeOH Blank 29 030199029 Tox 120 DNO. 1 3 6F -G 3,2/22/99 D2 30 030199030 Tox 120 DNO. 13 7 F -G 3 ,2/22/99 D2 31 030199031 Tox 120 DNO. 138F-G3, 2/22/99 D2 32 030199032 Tox 120 DNO. 1 3 9F -G 3,2/22/99 D2 33 030199033 Tox 120 DNO. 141F -G 3 ,2/22/99 D2 34 030199034 Tox 120, R BL02229-250ppb-CC3,2/22/99 35 030199035 MeOH Blank 36 030199036 Tox 120 DNO. 142F -G 3,2/22/99 D2 37 030199037 Tox.120 DNO. 143F -G 3,2/22/99 D2 38 030199038 Tox 120, RBL02229-250ppb-CC4,2/22/99 39 030199039- MeOH Blank 40 030199040 Tox 1 2 0 ,106F-G1-250ppb-MS, 2/22/99 41 030199041 Tox 1 2 0 ,106F-G1-250ppb-MSD, 2/22/99 42 030199042 Tox 120, RBL02229-250ppb-CC5,2/22/99 43 030199043 MeOH Blank 44 030199044 Tox 120, RBL02229-H2O B lk -2 ,2/22/99 45 030199045 .Tox 120, RBL02229-RBUverBlk-1,2 /2 2 /9 9 46 030199046 Tox 120, R BL02229-5ppb-2,2/22/99 47 030199047 Tox 120, RBL02229-1 Oppb-2,2/22/99 48 030199048 Tox 120, RBL02229-25ppb-2,2/22/99 49 030199049 Tox 120, R BL02229-50ppb-2,2/22/99 50 030199050 Tox 120, RBL02229>100ppb-2,2/22/99 51 030199051 Tox 120, RBL02229-250ppb-2,2/22/99 52 030199052 Tox 120, R BL02229-500ppb-2,2/22/99 53 030199053 Tox 120, R BL02229-750ppb-2,2/22/99 54 030199054 Tox 120, RBL02229-1000ppb-2,2/22/99
MS File
In let File
TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A . TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A ' TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TO X120A .-x TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A TOX120A
long2 long2 long2 long2 long2 long2 long2 long2 long2 k>ng2 long2 k>ng2 long2 . long2 long2 long2 long2 long2 long2 long2 k>ng2. long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 . long2 long2 tong2 k>ng2 long2 long2 long2 long2 long2 long2
B ottle
91
91
1 2
3 4 5
6.
7
8
9
10 11
91
12
13 14 15 16 17 92 18 19
20 21 22
23 92 24 25 26 27
28 29 93. 30: 31 32
93 33 34 35 93 36 37 38 39 40 41 . 42 43 44 45 46
Factor 1 Sampl
0 Analyti 0 Analyti 0 Analyti 0 Analyti 0 Stande 0 Stande 0 Stande 0 Stande 0 Stande 0 Stande 0 Stande 0 Stande 0 Stande 0 Analyti 0 Analyti 0 Analyti 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyt 0 Analyl 0 Analyt 0 QC 0 Analyl 0 Analyt 0 Analyl 0 QC 0 Analyt 0 Analyt 0 Analyl 0 QC 0 Analyl 0 Analyl 0 Analy 0 Stand 0 Stand 0 Stand 0 Stand 0 Stand 0 Stand 0 Stand 0 Stand 0 Stand
" [ Z u r . V \ |V \a J > 4 i V^U.
000573
FACT: DATA REVIEW SUMMARY FORM
BESTCOPYAVAILABLE
Date:
Analyst:
ci-rt
FACT StudyNumber ' T h 't I^ J Q
Analyte 4:
~$2A A
Acceptable Linear Range:
--' Jj
Matrix Time Points
toys l3
LOQ for this data set / D Re-evaluated for different range? f \ ) ^
Dilutions?
S ia -
Analyte 1:
Pfc^L
Acceptable Tihren*Range:
Analyte 5:
T - e J V T ^ M la i
Acceptable LinearRange^ / c o - J t C C D p f b
LOQ for this data set
LOQ fix Ibis data set: j b
Re-evaluatedfor differentrange?
f\)o`
Re-evaluatedfor different range? $ D
Analyte 2:
WEE
Acceptable Linear Range], / O ' l < ^ o p p b
LOQ for dns data set:
/O ffe
Re-evaluated fix different range?
(Jo
An junGaklxyjtve* v6.:
tpfah* ^'gJyw tv --SjCa*i/
Acceptable E faggf^ ^e:
LOQ for this data set
Re-evaluated fix different range? a /a*d _
feloh
cLclVc
0*
Analyte3: Acceptable Linear Range: " / OC& o p b LOQ fix this data set Re-evaluated fix different range? lO^
Analyte 7:
Acceptable LinearRange:
LOQ fix this data set
~~
Re-evaluated for differentrange?
000574
vs~^ o
t RS 7 " S
SSSSSSSaSSSSSSSSSSSSSSSSSSSa Fi2gS3SS3S3SSpg2Sg3^2S?2222S2ESSSi:SS33SSS?gSS2SSSSaS2SS s - o` d s i i s s g s g E S " ^ " o,3 d s o' s s s 3 d H " a ' " s a E " d S " s s 8 " , ; -;,,!a a 3 2 8 g g
V
2
IHHrIHHi
i|5 liM s ll||ip S S lla p lS S a il5 l 8 lp il|3 |ip llls ls i!||i,
> S :S H S p .p 3 S iS 3 p ie g :3 p S 3 s :S H iS S H S p ^ iiiiS S S S H p .
sssssssss s s s g g sssssssss
' s S S jj
i
i
S S
"SSSSSSS5
l | | | | l | ! ! p aaaaal aaaaag aaaaal ,, g | p P . | | | | | | | l |
Uh
4** i s f iS 32
h |s |3
Isis
iW
? I ! ~i i
!
3 s
S f f l W i ! 1 1 1 '* S ` - il! 'W W W I
S jj a a a a a a lla 3 | s a ||s ! s a ,| |8 |a j is .s ia i
8
a a S i S 5 ` S5's` 5 ' S ' 5 S ' a 5 5 ' a 5 5 a a 8 3 S 5 5 a 5 5 ' ; S 5 ' 5 ; S S S S 5 5 a 5 5 5 5
lIlSHlSlSSilllSHlSSllMlllSSll1
I V
g I W1
lli
liii)
lil
'S a a 3 3 3 S 5 S 3 S S a S S S S 5 S 5 S 2 S !3 2 S g P ;S 5 S i3 5 5 S S E S 3 S S S 2 S
W rs.
in o
o
o
1041098:: q u an titafcad and p rin ta d 0 3/08/99 by drb
9 r l t t N O l O H V r l 4H II I
i (4
1
a a 3S2232SSSS
s " -- 33333
4
H H H rrH H H H H
i H H S jg H
rl
a .s
r*-
3
NN1
a*> 2
*
i
w4 H H
! 3
u 3 S 3
SSSSoooIS
"SSS2SSSS
s
s
t
jf|piiiill11i|ff|| ******
I 3 s"
a aa ?aa
ro-
<on 3 3 ?
e o oMtf)oo<H O O f4<hO M 4
e lMA
H a la
-3 3 3 5 3 3 2 3
iii " S*5H|S,
5 K3S SS SS
s S S S s s S s K a 3o33ooSoo ' 'S S H jj
1
l ? p l | | | p sasaa s a s s s a a aSS ..i i! i i i i l i l i
3333' 3333
ffiiili lilil lilil lili
if'
1 * 51 I I 8 1 I I
41MM " SS * - 3 a a 3 3 3 3 3 S 3 3 * ag3a3s3s2a as;si a; 3a3s3s3s Ss 3s ^s ^s s s s s s s s s s a s s s a
< r ir>
o o o
141098:: q u a n tita ta d and p r in ta d 0 3 /08/99 b y d rb
TOX 120 BHO B a t L iv a r Ocoups 2 C I >041098! : q u a n ti t a t a d a n d p r i n t a d 0 3 /0 8 /9 9 lay 4 < t
J 7" '
1 533333
I sisals
3 m ai
J
.2
i s^ 2 3
tnH in
rH- 3 0 " ^ 7
3
3 s 333 **
333333
arlt mr- - s S S 3 lrAjoivnwtlBffNiH'
araitt
rn4
< 3 333
NNojrfntllOOoOQvleH
4 H *-t 4HH rlHHHHH
r-# 1 i s s s
H SSI3I
VMHOrtH(MiHwNrwt NAe
im
s s
* i m rin no nu n
. ... $. &
llBllllllallllllsilllallla
SS393S
sssssgsss
-sssssssis
3
3 s oH
-
isisasiiill....1 11aaaaal aaaaal aSaaal aal 111IpSpIllt
1
4 l l .......
a a a 5 S S S S 'S 5 S '= 3 3 5 S S S S S 3 S 5 a 3 3 S ';a 5 5 2 n S " 3 5 S
3 i i J < J 2 <<(<<3 l i 2 2 <<2 l < i J l l 3 l < i i < J i i l 2 2 J3 l I 2 1 2 l < l 2 2 l
p*
K
oIn o o
141098: : q u a n tita te d and p r in te d 03/08/99 by deb
O O
o
C/l
OB
8S88888 Ko)oKo) NoMooK o) N*M-U* ................... 5ir
1888 888 8 8 8 8 8 SS8 8 8 8 8 8 I
_ H* H* I--M H*
MO O O O O O
tH0QORO OtO4OMMV
* s i g m *S
S'b H I H *
NoKt*s t)H*H<JMU*WUP*4*UHM4)(H*A4
' K
NME*0IUMj iUaWU*UM*Um* ?KBnSBBmB
D* * * *
Ko *BBB
MoKoloKol MoNoMwViI ...................6 ! B S S S B B "'' SEBEES
BBBoB
ft*
7TJNJ KNJ KMJNt J JNJ
*.8)i>00 0W 00 0 w
SgBBBB
q vk. S k ks.kv.
H 0 0 00000
C>0B B 8 8 S
NMNMSMNNMNS MNS SS i00iH00i00.0ti
al ia
H00 00t t0W00NMOH0 t0NMH I )O OO O O I
MUa MeU0uU4AU*MUuUK0*)Uu1U0A
0 U
4a UNM
rIO* /
H HHM 3
4 K)
/NUUIOHUAU. Mt
A 0aaN *ea
HO HHH
Oh 4A*1 <400 OD U0U000 l00 e 00A0U0 OW0
0<4<40H HHHHHH
4HU4A 00M0*4<40 S&&SS5SS
e M4M4M*J SMO 4Hhi
S5& S
5 6
U0O000M0000OAM M0JlJ<<4J & H *m m
H 00 0W0NNHW N H 0000O 00M I
J: g i J14 UUKJM)l lW0U00U0WUWUUU0M
w*
mh
w
oo
H,,M
A0UH
wSSSStSIii HUVbH
00HO0M M MUHHAHAM
Ss?
MMg
5 ffI
Q uantify Coapound Suanaxy R aport
teat 120 DHO R a t L iv ac G roups 2 6 3
S aap la L i s t : M:SBAIS.8R0\.1aspTarB\030i99o
b a s t n o d i f i a d : Man M ar 08 0 9 :4 9 :2 1 1999
lia th e d :
M :\ratx.SRD\HathDB\030199a
b a s t n o d i f i a d : Mon M ar 08 1 0 :0 3 :3 2 1099
Job Coda:
P rintad:
Mon M ar 08 1 0 :3 3 :4 9 1999
Paga 3
Coapoiad S : TalaarlSO
Wane
f a lla l 1st ta rt
1 030199001 MaOH B lan k
2 030199002 MaOH B lan k
3 030199003 T o r 120, BBL02229-H20 B lk -1 , 2 /2 2 /9 9
1 030199001 Tox 120, BBb02229-BBbivarBlk-l, 2 /2 2 /9 9
S 03019900S Tox 120, BBM2229-Sppb-1, 2/22/99
6 030199006 Tox 120, B8I02229-10ppb-l, 2/22/99
7 030199007 Tox 120, BBL02229-25ppb-l, 2/22/99
0 030199008 Tox 120, 9BL02229-S0ppb-1, 2 /2 2 /9 9
9 030199009 Tox 120, HBL02229-100 p p b -l, 2 /2 2 /9 9
10 030199010 Tax 120, XB02229-250ppb-l, 2 /2 2 /9 9
11 030199011 Tox 120, BBl02229-500ppb-l, 2/22/99
12 030199012 Tox 120, BBL02229-750ppb-l, 2/22/99
13 030199013 Tox 120, m 0 2 2 2 9 -1 0 0 0 p p b -l, 2 /22/99
11 030199011 . HaOK B lank
15 030199015 Tox 120 DHO. 12SF-62, 2 /2 2 /9 9 DS
16 030199016 Tox 120 DHO. 126P-C2, 2 /2 2 /9 9 DS
17 030199017 Tox 120 DHO. 127F-G2, 2 /2 2 /9 9 D5
18 030199018 Tox 120 DHO. 129F-G3, 2 /2 2 /9 9 02
19 030199019 Tox 120 DHO. 130F-G3, 2 /2 2 /9 9 D2
20 030199020 Tox 120, BBL02229-2S0ppb-CCl, 2 /2 2 /9 9
21 030199021 MaOH B lank
22 030199022 Tox 120 DHO. 131H-G3, 2 /2 2 /9 9 D2
23 030199023 Tox 120 DHO. 1321-03, 2 /2 2 /9 9 D2
24 030199024 Tox 120 DHO. 13 3 r-C 3 , 2 /2 2 /9 9 D2
25 030199025 Tox 120 DHO. 13IF-G 3, 2 /2 2 /9 9 D2
26 030199026 Tox 120 DHO. 13SF-G3, 2 /2 2 /9 9 D2
27 030199027 Tox 120, RBb02229-250ppb-CC2, 2 /2 2 /9 9
28 030199028 MaOH B lank
29 030199029 Tox 120 DHO. 136F-63, 2 /2 2 /9 9 D2
30 030199030 Tox 120 DHO. 137 1 -0 3 , 2 /2 2 /9 9 D2
31 030199031 Tox 120 DHO. 138F-G3, 2 /2 2 /9 9 D2
32 030199032 Tox 120 SNO. 139F-G3, 2 /2 2 /9 9 02
33 030199033 Tox 120 DHO. 141F-03, 2 /2 2 /9 9 D2
34 030199034 Tox 120, RBb02229-2SOppb-CC3, 2 /2 2 /9 9
35 030199035 MaOH B lank
36 030199036 Tox 120 DHO. 112F-G3, 2 /2 2 /9 9 D2
37 030199037 ToX 120 DHO. 143F-G3, 2 /2 2 /9 9 D2
38 030199038 Tox 120, RBL02229-2S0ppb-CCI, 2 /2 2 /9 9
39 030199039 MaOH B lan k
40 030199040 Tox 120, 106F-01-250ppb-MS, 2/22 /9 9
41 030199041 Tox 120, 106F-G1-25Oppb-MSD, 2 /2 2 /9 9
42 030199042 Tox 120, BBb02229-2S0ppb-CCS, 2 /2 2 /9 9
43 030199043 - MaOH B lan k
44 030199044 Tox 120, BBL02229-H20 B lk -2 , 2 /2 2 /9 9
45 030199045 Tox 120, BBb02229-BBblvarBlk-l, 2/2 2 /9 9
46 030199046 Tox 120, RBb02229-5ppb-2, 2/22 /9 9
47 030199047 Tox 120, BBL02229-10ppb-2, 2/22 /9 9
48 030199048 Tox 120, HBL02229-25ppb-2, 2 /2 2 /9 9
49 030199049 Tox 120, BBL02229-50ppb-2, 2 /2 2 /9 9
SO 030199050 Tox 120, BBb02229-100ppb-2, 2 /2 2 /9 9
51 030199051 Tox 120, BBL02229-2S0ppb-2, 2/22 /9 9
52 030199052 Tox 120, BBb02229-500ppb-2, 2/22/99
S3 030199053 Tox 120, BBbO2229-7S0ppb-2, 2 /2 2 /9 9
54 030199051 Tox 120, BBW2229-1000ppb-2, 2 /22/9 9
*
S td Cono
A nalyt
RS
A nalyt
A nalyt
A nalyt
S ta n d s
5.99
S ta n d s
12.00
S ta n d s
29.90
S ts n d s
59.90
S tan d s 120.00 8.571
S tan d s 299.00 S.S49
S tands 599.00 8.593
S tan d s 898.00 8.562
S tan d s 1198.00 . 8.541
A nalyt
A nalyt
8.150
A nalyt
8.168
A nalyt
8.149
A nalyt
8.361
A nalyt
8.340
QC 2 9 9 .0 0 8.599
A nalyt
A nalyt
8.370
A nalyt
8.553
A nalyt
8.371
A nalyt
8.540
A nalyt
8.552
QC 2 9 9.00 8.559
A nalyt
A nalyt
8.352
A nalyt
8.331
A nalyt
8.572
A nalyt
8.568
A nalyt
8.585
QC 2 9 9 .0 0 8 .5 6 3
A nalyt
A nalyt
8.572
A nalyt
8.559
QC 2 9 9.00 8.568
A nalyt
A nalyt
8.580
A nalyt
8.560
QC 2 9 9 .0 0 8.5 0 5
A nalyt
A nalyt
A nalyt
S te n d a
5.99
S tatida
12.00
Stenda
29.90
S te n d a
59.90
Stenda 220.00 8.536
Stenda 299.00 8.550
S tenda 599.00 8.546
S tenda 698.00 8.505
Stenda- 1198.00 8.578
A rs a D U .F a c t. Cono F la g * %Dev
86 1 27S 1 372 1 631 1 727 1
311 1 301 1 266 1 329 1 418 1 173 1
281 1 4(5 1 260 1 352 1 500 1 163 1
391 1 380 1 408 1 357 1 328 1 262 1
257 1 218 1 222 1
226 1 196 1 190 1
119 1 209 1 393 1 535 1 703 1 X
8 5 .1 4 MM 4 1 9 .5 1 bbX 5 9 2 .4 3 MM 1 0 4 9 .4 9 MMX 1220.49 bb
4 8 3 .6 5 MK 4 6 5 .1 7 MK 403.94 bb 5 1 4 .8 4 'bb 7 2 5 .7 9 MK 23B .58 MK
430.52 bb 7 2 0 .9 0 MM 393.56 bb 5 5 6 .1 9 MM 8 ia .12 bb 2 2 0.94 MK
6 2 5 .6 7 MK 605.50 bb 6 5 4 .8 5 MK 564.48 bb 513.91 bb 3 9 7 .3 4 MM
3 8 8 .5 0 MM 319.39 bb 3 2 5 .7 9 MK
3 3 3 .8 2 MK 2 7 9 .8 7 MK 2 6 8 .6 1 MM
.
-29 40 -1 17 2
\ \ \
\I
-20 /
/I /1*b.-hwL-t. t_AJ.
r/ 0 3 / oj
-26 - \ \
\ I
A
33 | j
/ / 9_ //
-10
144.01 bb
302.82 bb 6 2 8 .6 2 MK 879.50 bb 1177.99 bb
20 1 5
-2 -2
% 3A t r ilt r ii, 7^1 (lloooil^ COsrk chicli. LS Wil'L'U. Cfiltrf.+ hrailhj.
ewwlo. Qflkft. is Ok fl/lA Urtjorialjli.,
W e - o j / o j lq<\
? /fi/Ojpfcc.
Hd^Usi
adelinaO ilQ O O : t q u a n tita te d a n d p r in te d 0 3 /0 8 /9 9 b y d rb
000579
4t a e cm t a m 1 1 CM 0 CM 0
1 1111
? sU
CM CM t a CM m 0 c e 0cm a
s1 0 H M A m i t a o h cm a 0 t a <-4 CM CM 0CO
II
t3a i HHHHHH
t a <0 49 40 49 m
H CM CM A t a O H CM CC 49 0 i f l
0 A 0 O 0 *4 S H N H N O
AAAAA
AO O O O O O O O g A O A A eeeoo 3 0CM A A O A AA A
4CM 0C M A A AA * 4 CM 0 A >4
3
C0
0t
33 taA 40
m CM CM 4P m H
CM CM
g
CM 0 A CM CM
ta 0
CM H 11
0
SS3
a wm 0 IM O
CM A A CM CM H
CM
S3
H CM tn 0
00
Ns
5S3
0 CM H aa e
CM CM O 0w m NHH
A A K) h,
CM CM 40 777
S 3 3 3
0 O CM t a CM 0 A t n 0 40 (M 40 0 0
0 0 CM CM A CM 0 CM 40 4 O . A * 4 *
H 0 0 0 ta
HH 0 40 40 0 CM H
H
ta
e M
A A 0 *4
AA
O e A A CM
CM CM
A
e 0 A A CM
H HH
40 A H M 0
0
*H H
00 00
O
HH H 0 ta ta 0 m cm 0 0 cm
H HH HH HH
0 CM CM 0 0 0 A 0 CM 0 t a A 0 CM
4 CM 40 * 4 t a
49 0 ta CM 0 CM
AAA
e e A A CM
OA CM CM
AA
0 ta 0
0N 0 AAA
Oe eA AA AA CM CM
ta H 0 0 0 0 e
00 0 <4 CM <4 0 0 000000 AAAAAAA
OOOOOOOOO AO AAO O O O O 0CM A A O A A00
4CM 0CM A A AA H N 00H
A A A t oAt AA A A
NNNSSNA
nN
A
Nn
A
Ms
S
nN
r-Sni
NM
S ! M CM
a a a *N N CM
0 OO0 O
rAIi ANfAAIAHINAIiAnI CCMM CCMMCMM CCMM
M CM CM CM CM .
aasaaa
iS iaaaa
h N N ta ta A
ssassam w R a o m
N CM S" 3 8 H N
NIMNCMN(MNNNCM
NNNN^
CM CM CM CM CM
m mptHaiNntaPntIaMntaCntMaCNA
tatatatataA
PlPIPlU^fj
H H H r l H *'
Il______
O O CI
MMIm n cm
iiiila*ggggga 3*gggggg
iwOOOOOo o e o o o O O H O O O O O O r
CM CM CM CM CM CM C M C M M C M C M C M C M C M C M I
HrlHHHH a HHHH sasass s a s - s a s ;H H
Hn H H HH t
CM CM CM CM
t3a H3 3H843N3Ht3a
8
ta
1
X
t
3
at
8
at
8
at
3
at
8
at
81
aS
t3at8a8ta8ta8ta8ta j5H3 K3 H3 HS H3 HS S MS 3> K8 f K3K3h8:;
t CM
A
CM
AA AA A
I NS Ao A WA AS AN AS SA N*Sk
I A SSN N N N N N
N N M C iC C P IC C fC 's
IH N N N N N N S S N
I CM S N S CM CM CM
+ M CM M CM
li- ft a
** >
M CM CM CM CM
I H CM CM CM `
tatatatatas*ai isisisisis**t r -
m mA A A A A A CM CM CM CM CM CM CM CM CM CM 4 CM
3333333333 --gasaaaaa _OOOOOOOOO
CMCMCMCMCMCMCMCMCMCM HHHHHHHHHH
88 8 3 8 3 3 8 3 3 3
HNH OV
I O, O H N P I M
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAoOA oOA oOA oOA oOA oOA eOAoOAwOA -OA. OAo OA' A A A A A A A A A A A A A A A A AOx .O........O........O........O........O........O........O........O........O.......-O ....O........O........O........O........O........C.......-. A_ A--A_ A--A-- A--A--A_ A--A--oA--o Ao- Ao Ao Ao oA oA cA A A A A A A A
H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H oooooooooooooooooooooooooooooo ooooooooooooooooooooooo
i J rp--i i --O --f i -P ^> foi Po i on no poi oo Po Ho i Po oP oi Po i o Po Ho i ro t oP o r ot Po i o ioP oi 5of o 3o0 o2 2o 2o2 o ? o2 2o 2o 2o o2 o 2 o2 2o 2o So 2o o2 io2 o2 o2 o2 0o o2 2o 2o2
|N P |M C IM h A O H N P I ill< B h B A e H N P IM O I0 M }a e H N P IM iO V h a O lO H N n M ) r 4 H H H H H H H N N C I N M N P I W P | P | P | P J P l P | P | P H l1 P I M M M M M M M M M M i l H O l l l i n
O 00 in o o o
W *4 66/80/0 Wtrptd f a pawnTM** ::S60TI
141098:: q u a n tita ta d and p r ia ta d 01/08/99 b y dxb
saBSSSSaSSSCSSSSSiSSSSSSSJSSKlgKSSSSKCKPS.-^.-.
r I
m m m m m iw iiw m m w iw w im m m m u
gsggSSgSSgggggssssgsssSssgsgSsgBSsgsSsggsssgssgggsggssg
i* ; ;
r BBv * &V a V a &a &&*
f
!I1IB ilii IBJ % o * o % o * *
s IIPX r r r r f**i*'l
!lllllll5l sil Ssssss
p
p
= bb
S s b ' b S b b b b b B-
bbbbb -
1111
til?
SSS-
S5SSS
K!
s
i
sssssssss
n* OUe N(tNUOMOtM*fJ
IfEKH
&&****
VHHHMMHHM Oo Oo Oo Oo Oo Oo Oo Oo oO
NV UO N tNf WH OUOU>NJNOU UU
O
o o en
HH NH WH NH NO Hb^NO
H 0O
0
MHHMKHMHH H N H M N H H M O U I*
9 H
9 O
Hs
0
*#
HM
(?V
0 0 e o e OHOHHOOMOOO
SSSP3S3K32
#- aas
0o 0UkOM-oNObU>WNOt9fa9H0
S333535S33*
3 3 3 33333333333
wi M iU,l w u .~
1
Q uantify C a lib ra tio n R aport
rox 130 BHO R a t L iv a r Seoupa 3 8 3
1 A llib ra ti on: M:\xata.SRO\CurvaDB\010199a ' M t n o d i i i a d : Mon Mar 08 1 0 :1 0 :0 7 199*
Printed:
Mon Mar 09 1 0 :3 S :0 S 1*99
I
]
]
]
T
]
i
i
J
3
]
1
!
J
I idalinaO U .098 : : q u a n tita te d an d p r in t a d 0 3 /0 8 /9 9 b y d rb
i
Raga 1
000582
Q u an tify C a lib ra tio n K ap ert
TOC 1 2 0 aio K a t l i v m r C ro u p s 2 C 1
C a lib r a tio n : M :\sats.9B0\CurvaEB\O3O199e
l a s t m o d ified : lion Iter 00 11:0<:32 190
P rinted:
Mon Mas 08 1 1 :1 0 :0 7 1998
Paga 2
aa d elin aO 41098 : : q u a n tita te d and p r in te d 0 3 /0 8 /9 9 b y dab
000583
1
ifcify C a lib ra tio n E ap o rt 120 DHO S a t U v a s O roupa 2 0 3
1 a l it e r a t i o n : M: \ r a t a .JUACurva0B\030199o a a t a o d if la d : Mon H as 08 1 0 :1 0 :0 7 1980
T intad:
I t e Mas 08 1 0 :3 8 :0 9 1399
1
I
]
1
T
1 .1
i
I
]
J
]
]
.1
J
] m ad alin a0 4 1 0 9 8 : : q u a n tit a ta d an d p r in ta d 0 3 /0 8 /9 9 b y deb i
Vaga 1
000584
Q u a n tify C a lib ra tio n Rapor t TOX 1 2 0 DHO R a t U v a s S c a u p s 2 C 3
C a lib ra tio n : M :\cats.9RO\CumOB\03Q199a
L a s t a o d ifia d : Mm M u 08 10:10:07 1908
P rintad:
Mon MU 08 1 0 :3 0 :0 3 1899
Compound 4 name: POAA Correlation coefficient r = 0.998817, r*2 = 0.997636 Calibration curve: 5.20938 * x +18.1144 Response type: External Std, Area Curve type: Linear, Origin: Exclude, Weighting: Null, Axis trans: None
Pag* 4
m * d u lin a 0 4 1 0 9 8 :$ q u a n t i t t a d a n d p r i n t e d 0 3 /0 8 /9 9 b y dadi
000585
Quantify Calibration Hapcct TOX 120 OHO Hat Livar Sroup 2 c 3
Calibration: M: \rata.9H0\CuxvuCB\010199a
last aodiflad: Mon Mar 03 10:10:07 1399
Prlntad:
Moo Mar 08 10:38:03 1999
paga 5
aadalina041098:: quantitatad and psiatad 03/08/99 by drb
000586
1
lu an tify C a lib ra tio n R apact fCflC 1 2 0 OSO R a t U v n C r o u p * 2 ( 3
1 la lib r a tio n ; M :\rats.IR O \C u rv tfB \0 3 0 1 9 9 a * * t a o d i f i a d : Mon Max 00 1 0 :1 0 :0 7 1900
fein tad :
Mon Max 09 1 0 :3 0 :0 3 1990
1 C om pound 6 nam e: Telm er569 C orrelation co efficien t r = 0.997982, r* 2 = 0.995968 C alibration curve: 2.28567 * x + -17.6540
1 R esponse type: E xternal Std, A rea C urve type: Linear, O rigin: E xclude, W eighting: N u ll, A xis trans: N one
]
j
1
1
i
I
]
J
3
]
]
I
]
1 td elin * 0 4 1 0 9 8 :: i{ u a n tiU ta d and p r tn ta d 03/0 8 /9 9 b y drb
ii
Saga (
000587
Q u an tify C a lib ra tio n B a p e rt TOX 1 2 0 OHO B a t U n r C r o u p s 2 0 3
C a lib r a tio n ! M :\rats.B 8 0 \C u rv aB B \O lO 1 9 9 o
L a a t a o d if ia d : Mon M ar 01 1 0 :1 0 :0 7 1390
B rin ta d :
Mon M ar 00 1 0 :3 0 :0 9 1999
7
aadalina041098:: q u a n tita ta d and p rin ta d 01/08/99 by dsb
000588
M ethod R e p o rt
M ethod f i l e ! L a st m o d ified : Compound:
P rin te d :
M :\rats.P R O V M athD B \030199e.m db Hon M ar 09 1 0 :0 3 :5 2 19990
1 : P90S
KOn M ar 09 1 0 :1 0 :0 2 1999
Compound Hio u i In tarn al R af.:
Q uantify Traca: Cone, o f S tandards:
U sa r RF V a lu o : Usar Paak F actor: I n t a g r a t a Window F a c to r :
Paale L o c a tio n
R atantian Tina (B ins): RaXativa R atan tio n T ina: T in s Window (m in s):
Paale S s ls c tl o n :
Raaponaa
Rasponsa Typa: Raaponaa:
C a lib ra tio n Curvaa
Polynom ial Typa: P o in t o f O rigin: F i t W aighting: A xis Tranaform ation:
C oncantratlon
Units:
Kola a
Paak-to-paak am plituda:
SBOOth
Sm oothing: Window a i z a ( s c a n s ) : a Huabsr o f aaooths: Smoothing mathod:
B aaallnas
Jo in v a lla y thrashold: Raduca paak ta ilin g : R a is a b a s a l i n a maximum: v
Paak S aoaration
Draw v a r t i c a l th r a s h o ld : D atact shouldars:
Shouldar th ra sh o ld :
Rasponsa Thraahold
R alativ a H aight: A bsoluts H aight: R alativa araa: A bsoluts araa:
nos (Mona) TIC Cone. A 1.000000, D isablad
1.000000
2.000
0.130, Inablad 0.000, D isablad 0.793 F aarast
S x ta rn a l A raas
L in aa r Ix c lu d a Rons Mona
150
D isablad 3 2
2 0 .0 0 100.00 0.00
10.00 D isablad 10.00
1.50, D isablad 0.00, D isablad 2.00,* D isab la d 00.00, Inablad
Peg* 1
OHI0W Uc o i l o s h i
A
000589
M ethod R e p o rt
M ethod f i l e : t e a t m o d ified : Compound:
P rin te d :
H :\nto.IIl0\ltethia\0301M e.adb Hon H er OS 1 0 :0 3 :5 2 19990
2: m s
Hon H er 09 1 0 :1 0 :0 2 1999
Compound Mama: In tern al R ef.:
Q uantify Traee: Cone, o f Standards:
PfHS (Nona] TIC Cone. A
U sa r RF V a lu a : Usar Paak fa c to r: I n t e g r a t e Window f a c t o r :
Paak Location
R eten tio n Time (m ine): R e la tiv e R eten tio n Time: Time Window (m in s):
Paak S election:
Response
Response Type: Responses
1.000000, Dlsabl
.1.000000
2.000
5.732, In ib lad 0.000, D ijablad 1.109
V aarast
' Ixtam al A reas
C a lib ra tio n Curves
Polynom ial Types Point o f O rigin: f i t W eighting: A xis Transform ation:
L in ea r Include None Stone
C oncentration
U nits:
N o ise
Peak-to-peak am plitude:
290
Smooth
Smoothing: Window s i z e ( s c a n s ) : . Number o f sm ooths: Smoothing method:
D isabled 3
2
B aselines
Jo in v a lle y threshold: Reduce peak ta ilin g : R a ise b a s e l i n e maximum:
0.00 100.00 0.00
Peak Seoaration
Draw v e r t i c a l th r e s h o ld :
10.00
D etect shoulders:
D isabled
Shoulder th re sh o ld :
40.00
Response Threshold
R elative h eight: A bsolute h e ig h t: R elative are: A bsolute a rea:
1.50, D isabled 0.00, D isabled 2 .0 0 / D isabled 10.00, Enabled
Page 2
Mank lw.. cwoy______ i m .oiIm Ivi
000530
M ethod R e p o rt
M ethod f i l e : L a st m o d ified : Compound: P rin te d :
M:\ r a t s . PR O \H ethD B \0 3 0 1 9 9 e.m d b Kon M ar 00 1 0 :0 3 :5 2 1999Q
3:
Kon Mar 08 1 0 :1 0 :0 2 1999
Compound Hi m : In ta m a l Ref. i
Q uantify' Trace: Cone, o f Standards:
[Mona] TIC
Cone. A
V sar HI V alua: Vsar Paak F actor:
I n t a g r a t a Window T u t o r t
1.000000, D isab) 1.000000 2.000
Paak Location
R atantion Tiaa tain s):
R alativ a R atantion Tina) T in a Window (n in a )i
1.103, Xnablad 0.000, D isablad 1.000
Tank S o lu tio n :
B aaraat
Response
Response Typai Response:
K xtarnal A raas
C a lib ra tio n Curvaa
Polynom ial Typa: P o in t o f O rigin: f i t W eighting: A xis Transform ation:
L in ea r Ixclw le
Bone Bene
C oncentration
U nits:
M oiaa
Peak-to-peak am plitude:
1
Smooth
S m oothing: Window s i z e (s c a n s ):
Humber o f sm ooths: Smoothing method:
D isabled 3 2
B aselines
J o in v a lla y thraaK old: Raduca paak ta ilin g : R aisa b a sa lin a ariwna: 1
Task S atu ratio n
20.00 100.00 0.00
Draw v e r t i c a l th re s h o ld : D ataet shoulders: Shoulder th resh o ld :
10.00 D isabled
0.00
Response Threshold
R alativa haight: A baoluta h aig h t: R alativa azaai A baoluta araa:
1.50, D isablad
0.00, D isablad 2 .0 0 ,'D isablad 00.00, Xnablad
Rag 3
M a c U .W , DM1DM
A t. m / m / w
000591
M ethod R e p o rt
M athod f l l : L a s t m o d ified :
Com pound: P rin te d :
H :\rats.P R 0 \H eth D B \0 3 0 1 9 9 c.m d b Hon Ite r 06 1 0 :0 3 :5 2 19990
I : POM
Mon M ar 08 1 0 :4 0 :0 2 1999
Compound N ana: In tarn al R ef.:
Q uantify Trace: Cone, o f S tandards:
PQAA [None] .
TIC Cone. A
U s e r RP V a lu e :
U ser Paak fa c to r: I n t g r a t Window F a c to r :
1.000000, D isabled
1.000000
2.000
Paak Location
R etention Tina (n in e): R elativ e R etention Tina: T in e Window ( n in e ) :
6.624, Snabled 0.000, D isabled
1.000
Paak Selection:
D earest
Response
Response Type: Response:
In te rn a l A reas
C a lib ra tio n Curve
Polynom ial Type: P o in t o f O rigin: l i t W eighting: A xis Transform ation:
L in ea r Kxclude Done Hone
C oncentration
Units:
P o is e
Peak-to-peak am plitude:
107
Smooth
Sm o o th in g : Window s i z e ( s c a n s ) : .
Humber o f sm ooths: Smoothing method:
D isabled 3
2
B aselines
Jo in v a lle y th resh o ld : Reduce peak ta ilin g :. R a is e b a s e l i n e maximum:.*
2 0 .0 0 100.00 0.00
Peak Separation
Draw v e r t i c a l th re s h o ld : D etect shoulders: Shoulder th re sh o ld :
10.00 D isabled
. 40.00
Response' Threshold
R elative h eig h t: A bsolute h e ig h t: R elative area: A bsolute are a:
1 .5 0 , D isabled
0.00, D isabled
2 .0 0 ,
`D isabled
60.00, D isabled
Pag 4
MadfeL, omoqj___ ik e . m I m / m
:X
000592
M ethod R e p o rt
M ethod f i l e : L a st m o d ified :
Coapound:
P rin te d :
M :\rats.PRO\M ethDa\030199c.m db Ksn Mer 08 10:03:32 19990
3 : T elm art< 9
Mon M ar 08 1 0 :1 0 :0 2 1999
Coapound Hama: I n ta rn a l Hof: Q uantify Traca: Cone* o f S ta n d a rd s :
U s e r MT V a lu e : User Peak F actor: I n t e g r a t e Window f a c t o r :
Peak lo c a tio n
R etention Tine (B ins): R elativ e R etention Tine: Tima window ( n in s ) :
Paak Salaction:
SUsDcnsa
Rasponsa Typa: R tapons:
C a lib ra tio n Curvas
Polynom ial Typa: Point o f O rigin: f i t W eighting: A xis T ransform ation:
C oneantration
u n its:
K o isa
Peak-to-peak am plitude:
Smooth
S m oothing: Window s l z a ( s c a n s ) : . Nunbar o f sm ooths: Smoothing nath o d :
B asallnas
Join v allo y threshold: Radueo peak t a i l i n g : R a isa b a s e l i n e maximum:.'
Paak S a tu ra tio n
Draw v a r t i e a l th ra s h o ld x D atact sh o u ld ars: Shouldak th ra s h o ld :
kasDonsa T hrashold
R alativa h aig h t: A bsoluta h a ig h t: k alativa araa: A bsoluta a ra a:
Talaar469 IHona) TXC Cone. A 1.000000, D isabl 1.000000 2.000
0.370, Rnablad 0.000, D isabled 1.000 V aarast
a In tern al A reas
L ln aa r Ix c lu d a Kona Hona
169
D isablad 3 2
20.00 100.00 0.00
10.00 1 D isabled
. 0.00
1 .50, D isabled 0.00, D isabled 2.00,' D isabled 00.00, Rnablad
Macl&Li QKtoM \ A c b ih x lw
Page 3
000593
M ethod R e p o rt
M ethod f i l e : L est m o d ified :
C om pound:
P rin te d :
M: \ra ts.P R O \M sth D 8 \0 3 0 1 9 9 c.a d b Mon Mar 08 1 0 :0 3 :5 2 1099a
6: T e la e rS < 9
Mon M ar 08 1 0 :1 0 :0 2 1999
Compound Ham! In tern al R tf. s Q uantify Traea: Cone o f S ta n d a rd s )
T alm ar569
[Hone] TIC Cone. A
U se r R7 V a lu e : User Peek F actor: I n t e g r a t e Window f a c t o r :
1.000000, D isab led 1.000000 2.000
Saak Location
R etention Tine (nlns): R elative R etention tin e : T in e Window (m in s):
9.818, Bnabled 0.000, D isabled 0.500
Peak S electio n :
V earest
Resocnse
Response Type: Response)
E x tern al A reas
C a lib ra tio n Curves
Polynom ial Type:
Point o f O rigin: P it W eighting: A xis Transform ation:
L in ea r Exclude Home Rene
C oncentration
U nitst
B o ise Peak-to-peek an p litu d a:
.
169
Smooth
Smoothing: Window s i c e ( s c a n s ) : Kuaber o f smooths: Smoothing method:
D isabled 3 2
B aselines
Jo in v a lle y threshold: Reduce peak t a i l i n g : R a is e b a s e l i n e maximum: .
20.00 100.00 0.00
Peek Separation
Draw v e r t i c a l th re s h o ld : D etect shoulders: Shoulder th re sh o ld :
10.00 4 D isabled
40.00
Response Threshold
R elative h eig h t: A bsolute h e ig h t:
R elative area: A bsolute a re a:
1.50, D isabled 0.00, D isabled 2 .0 0 ,.D isabled 00.00, Inabled
MorlA). CH UM ___ LAC- Q l l p t f a
leg e 6
000594
M ethod f i l e : L e st m o d ified :
Compound:
P rin te d :
H :\rata.P R O \H eth IIB \0 3 0 1 9 9 c.m d b Mon M u 01 1 0 :0 3 :5 2 19990
7: su rr
Mon M er 01 1 0 :1 0 :0 2 199
Compound V iH t I n tu n e l K ef.: Q uantify Trace: Cone, o f S tandards:
U se r RP V a lu e: User Peak Factor: I n t e g r a t a Window T a c to r :
Peak lo c a tio n
R e te n tio n Tima (m ins): R e la tiv e R eten tio n Time: Time Window (m in s):
Peak S election:
Response
Response Type: Response:
C alib ratio n curves
Pclynom ial Type: Point o f O rigin: T it W eighting: Axis T ransform ation:
C oncentration
U nits:
B o ise
Peak-to-pak am plitude:
Smooth
soothing: Window s i z e ( s c a n s ) : Humber o f sm o o th s: so o th in g method:
B aselines
Jo in v a lle y threshold: Reduce peak ta ilin g : R aise b a s e lin e maximum:
Peak Seoaration
Draw v e r t i c a l th r e s h o ld : D ateet shoulders: Shoulder th resh o ld :
Response Threshold
R slative height: A bsoluts h e ig h t: R elative aree: A bsoluts a rsa:
s u rr [Bone) TIC U sed 1.000000, D isabled 1.000000 2.000
7 .3 0 0 , Xnabled 0.000, D isabled 1.000 H auest
' Internal A reas
lin ear Include Bo m Hens
169
D isabled 3 2
20.00 100.00 0.00
10.00 D isabled 10.00
1.S0, D isabled 0.00, D isabled 2*00,. D isabled 00.00, b a b ie d
M a A t L - Mici? u c oiloaln
000595
AM ELIA062498: FACT Equipm ent L og 98061
Q uattro n , #6448E
PAGE
DATE: 03/16/99
ANALYST: hoi ' STUDY NUM BER to xl20. MATRIX: R at liver D ESCRIPTION; ion pair dilutions
FACT M ethod: FACT-M -4.0
Injection volum e.10 uL
Cycle time: 13.5 m in.
Electrode: Z-Spray
M obfle P hase:
(A12.0mM NH4CH3CQ2
(BIM eO H
TN -A - or stndrd num ber (A ) 98094-15-20________
. (B1TN-A-2325
Prepared (date/by): E x p ires:
( A) 3/16/99 hoi__________ ( A) 09/99_______________
0313/16/99 hoi (S ) 9/16/99
Store at room tem p? Q o r N
O ther
G u ard colum n: N one___________________
P art num ber: na_________
Lot number na
A nalytical colum n: keystone b e ta sil___________;__ Size: 2 x 100mm________________ Type: c l 8___________ _ Sphere size: 5 um Part num ber 105-701-2_______ L otnum ber__JP8H 08 Serial num ber 02949SD
A nalytical param eters:
Drying gas (L /hrl: 300
ESI nebulizing gas (IVhr) 15
LC Flow rate: 300 uL/m in. B ackpressure: 100 b ar
Sheath gas (L/hr)_0___
HPLC solvent gradient: long2
Tim e (m in.) 11 0.00________ 21 8.5 3 11.0_________ 51 12:5 6113.5_________
%B 40 90 90 40 40
000596
^ Tuning Method Report
: ________
Method:
C :\MASSLYNX\RATS2.PRO\ACQUDB\PFOS
Printed:
Tue Mar 16 09:59:43 1999
_ ___________________________________ _____ _____________
.I
A to L * n i M V i
Page 1
iSTCWmtlMLE
MS
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Dau 499.00
Set Rdbk
3.00 60
5 0.2
150 .250
2.99 -60 -6
150 258
Pressures
Analyser Vacuum Gas Cell
Rdbk
1.0e-4 4.5e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
.*S.
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0 2.0? 0.0 ' 56 650
Set Rdbk
15.0 15.0 45 2.0
0 .0
40
0
650
46
38 46
000597
S c a n n in g M ethod S o p o ri
M ethod:
C:\HMStTJOC\RAI32. s R o u c o u B a w r o a - p n s -M
L a a t M o d ifie d : Mem M ar IS 1 3 :2 3 :3 3 1999
P rin te d :
Too M ar 1 1 0 :3 3 :3 1 IM S
Solvent D elay < a la s ) i
0 .0 0
function : 1
MRM o f 4 M aes f a i r s ( ZAP- )
I n te r Channel D elay ( Secs ) l
Span ( D eltona I t
S ta r t Tine ( K ins 1 t End T ine ( K ins ) i
R ip ilti S Channel P aren t D aughter
0.03 0.00 0 .0 0
13.30 1 D w ell (S ees) C o ll E n e rg y (eV)
Cone < V )
1
499.00 0 .0 0
0.40
2
499.00 99.00
0 .4 0
3 499.00 130.00 0.40
4 499.00 180.00 0.40
45 4S 45 45
0 SO SO SO
Function t 2
MRM o f 4 M ass f a i r s 1t S f - 1
In te r Chennai D elay I Secs ) i
Span ( D altons ) : S ta r t T ina ( Kina 1 : End T ine ( H ina ) I R epasts :
Channel P aren t Daughter
0 .0 3 0 .0 0 0 .0 0 13.30 1 D w ell (S ecai C o ll E n e rg y (eV)
Cone ( V )
1
3 9 .00 0 .0 0
0 .4 0
2
399.00 99.00
0.40
3 399.00 130.00 0.40
4 399.00 180.00 0.40
43 43 43 < 43
<0 so
so so
lunation i 3
MM o f 1 Mass f a i r ( ESP- )
In te r Channel Delay ( Seca I : Span ( D altons > :
S t a r t T in a < Mina ) t End T ine { Mina ) :
Repeats :
Channel P aren t D aughter
0.03 0.00 0 .0 0
13.30
1 Dwell
(Seca)
C oll Energy
(aVJ
Cone ( v )
1
421.00 0 .0 0
0 .4 0
40
00
L& V
LAC. osL h
fags 1
t*r
000598
BESTCOPYAVAILABLE
jjk \ l tfa r M asslynx Sam ple List
Sample List: C:\M ASSLYNX\rats2.PRO\Sam pleDB\031699b.SPL
Printed: Tue M ar 1614:40:11 1999
ArUc
DUW
Page 1
File Nam e
File Text
MS File
In let File
1 031699001
MeOH Blank
PFO S-PFHS-IS
long2
2 031699002
MeOH Blank
PFO S-PFHS-IS
long2
3 031699003
Tox120, R B L02229-H 2O B Ik-2,2/22/99
PFO S-PFHS-IS
long2
4 031699004
Tox120, R B L02229-U vBlk-2,2/22/99
PFO S-PFHS-IS
long2
5 031699005
TOX120, RBL02229-5ppb-2,2/22/99
PFO S-PFHS-IS
long2
6 031699006
Tox120, RBL02229-1 Oppb-2,2/22/99
PFO S-PFHS-IS
long2
7 031699007
Tox120, RBL02229-25ppb-2,2/22/99
PFO S-PFHS-IS
long2
8 031699008
Tox120, RBL02229-50ppb-2,2/22/99
PFO S-PFHS-IS
long2
9 031699009
Tox120, RBL02229-1OOppb-2, 2/22/99
PFO S-PFHS-IS
long2
10 031699010
Tox120, R BL02229-250ppb-2,2/22/99
PFO S-PFHS-IS
long2
11 031699011
Tox120, RBL02229-500ppb-2,2/22/99
PFO S-PFHS-IS
long2
12 031699012
Tox120, R BL02229-750ppb-2,2/22/99
PFO S-PFHS-IS
k>ng2
13 031699013
Tox120, RBL02229-1000ppb-2,2/22/99
PFO S-PFHS-IS
long2
14 031699014 15 031699015
MeOH Blank T o x 1 2 0 ,1 6 1 F -G 5 ,2/22/99, d250
PFO S-PFHS-IS PFO S-PFHS-IS
long2 long2
16 031699016
T o x120,162F -G 5,2/22/99, d250
PFO S-PFHS-IS
long2
17 031699017
T o x120,163F -G 5,2/22/99, d250
PFO S-PFHS-IS
long2
18 031699018
T o x120,164F -G 5,2/22/99, d250
PFO S-PFHS-IS
k>ng2
19 031699019
T ox120,165F -G 5,2/22/99, d250
PFO S-PFHS-IS
tong2
20 031699020
Tox120, RBL02229-250ppb-CC2,2/22/99 PFO S-PFHS-IS
long2
21 031699021
MeOH Blank
PFO S-PFHS-IS
long2
22 31699022
Tox120,166F-G 5, 2/22/99, d250
PFO S-PFHS-IS
long2
23 031699023
T o x120,167F -G 5,2/22/99, d250
PFO S-PFHS-IS
long2
24 031699024
T o x120,168F -G 5,2/22/99, d250
PFO S-PFHS-IS
long2
25 031699025
Tox120, RBL02229-250ppb-CC3,2/22/99 PFO S-PFHS-IS
long2
26 031699026
MeOH Blank
PFO S-PFHS-IS
long2
27 031699027
T o x1 2 0 ,1 7 7 F -G 6 ,2/22/99, d1000 .
PFO S-PFHS-IS
long2
28 031699028
T ox120,1787F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
29 031699029
T o x120,179F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
30 031699030
T o x120,180F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
31 031699031
T o x120,181F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
32 031699032
Tox120, RBL02229-250ppb-CC4,2/22/99 PFO S-PFHS-IS
long2
33 031699033
MeOH Blank
PFO S-PFHS-IS
long2
34 031699034
T ox120,182F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
35 031699035
T o x120,183F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
36 031699036
T o x120,184F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
37 031699037
T ox120,185F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
38 031699038
T ox120,186F -G 6,2/22/99, d1000
PFO S-PFHS-IS ' long2
39 031699039
Tox120, R BL02229-250ppb-CC5,2/22/99 PFO S-PFHS-IS
long2
40 031699040
MeOH Blank
PFO S-PFHS-IS
long2
41 031699041
T ox120,187F -G 6,2/22/99, d1000
PFO S-PFHS-IS
k>ng2
42 031699042
Tox120,169F-G Q , 2/22/99, d1000
P FO S -P FH S -IS . k>ng2
43 031699043
T o x120,190F -G 6,2/22/99, d1000
PFO S-PFHS-IS
long2
44 031699044
T o x i2 0 ,191F -G 6 ,2/22/99, d1000
PFO S-PFHS-IS
long2
45 031699045
Tox120, RBL02229-250ppb-CC6,2/22/99 PFO S-PFHS-IS
long2
46 031699046
MeOH Blank
PFO S-PFHS-IS
long2
47 031699047
T o x1 2 0 .1 6 9 F -G 5 ,2/22/99 .
PFO S-PFHS-IS
long2
48 031699048
T ox120.170F -G 5,2/22/99
P FO S -P F H S -IS
long2
49 031699049
T o x120.171F -G 5,2/22/99
PFO S-PFHS-IS
long2
50 031699050
Tox120, RBL02229-250ppb-CC5, 2/22/99 PFO S-PFHS-IS
long2
51 031699051
MeOH Blank
P FO S -P F H S -IS
long2
52 031699052
T ox120.173F -G 5,2/22/99
PFO S-PFHS-IS
!ong2
53 031699053
T o x120.174F -G 5,2/22/99
PFO S-PFHS-IS
long2
54 031699054 . T o x120.175F -G 5,2/22/99
PFO S-PFHS-IS
long2
55 031699055
Tox120, RBL02229-250ppb-CC6,2/22/99 PFO S-PFHS-IS
k>ng2
56 031699056
MeOH Blank
PFO S-PFHS-IS
long2
57 031699057
Tox120, RBL02229-H2O BIk-2,2/22/99
PFO S-PFHS-IS
long2
58 031699058
Tox120, R BL02229-UvBlk-2,2/22/99
PFO S-PFHS-IS
tong2
59 031699059 60 031699060
Tox120, R BL02229-5ppb-2,2/22/99 Tox120, RBL02229-10ppb-2,2/22/99
PFO S-PFHS-IS PFO S-PFHS-IS
Iong2 long2
B ottle
91 91 1 2 3 4 5 6 7 8 9 10 11 91 12 13 14 15 16 82 91 17 18 19 83 92 20 21 22 23 24 84 92 25 ^ 26? 27 28 29 85 92 30 31 32 33 86 93 34 35 36 85 94 37 38 39 86 94 1 2 3 4
000S99
V olum e Sar
10 Ani 10 An: 10 Ani 10 An< 10 Sta 10 Sta 10 Sta 10 Sta 10 Sta 10 Sta 10 Sta 10 Sta . 10 Sta 10 Ani 10 Ani 10 Ani 10 Ani 10 Ani 10 Ani 10 QC 10 . Ani 10 An; 10 Ani 10 Ani 10 QC 10 An; 10 Ani 10 Ani 10 An; 10 An; 10 An 10 QC 10 An 1 0 : An 10 An 1 0 An 10 An 10 An 10 QC 10 An 10 An 10 An 10 An 10 An 10 Q( 10 At 10 Ar 10 Ar 10 Ar 10 Q< 10 Ar 10 Ar 10 Ar 10 Ar 10 Qi 10 Ar 10 Ai 10 Ar 10 SI 10 SI
KSTCWmiUBlf
b) 3 itef&9____ ;__________ ;____________ ______________ ;______
M asslynx - Sam ple List
Sam ple List: C :\M ASSLYNX\rats2PRO \Sam pleDB\031699b.SPL Printed: Tue M ar 1614:40:11 1999
0L 2W
Page 2
lA C P s/lolH
F ile Nam e
61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067
F ile T ext
TOX120, R B L02229-25ppb-2,2/22/99 Tox120, R B L02229-50ppb-2,2/22/99 Tox120, R B L02229-100ppb-2,2/22/99 Tox120, R B L02229-250ppb-2,2/22/99 Tox120, R B L02229-500ppb-2,2/22/99 Tox120, R BL02229-750ppb-2,2/22/99 Tox120, R B L02229-1000ppb-2,2/22/99
MS File
P F O S -P F H S -IS P F O S -P F H S -IS P F O S -P FH S -IS P F O S -P FH S -IS PFO S-PFHS-IS P F O S -P FH S -IS P F O S -P FH S -IS
In let File
k>ng2 Ing2 long2 long2 long2 long2 long2
B ottle
5 6 7 8 9 10 11
Volum e Sai
10 Sta 10 Sta 10 Sta 10 Sta 10 Sta 10 Sta 10 Sta
# I
000600
FACT: DATA REVIEW SUMMARY FORM
Date:
3/nlqq
Analyst: FACT Study Number
AC,
T o k -120
Matrix Time Points
i d L L n/er
fii-ppps. Gs-rm
Dilutions?
y * / * p - -----------
Analyte 1:
UeJa)i'ttA Ibur Acceptable LinearRange:
1
LOQ far this data set:
9 1 m ---------- .
Re-evahted fix differentrange?
Alo
Analyte 4: Acceptable Linear Range: LOQ for this data set: Re-evaluated for different range?
Analyte 5: Acceptable Linear ] LOQ for this data set. Re-evaluatedfor differentrange?
Analyte 6: Acceptable! anear Range: LOQ for thf< data set Re-evaluate*1for differentrange?
Analyte 3: Acceptable Linear Range:
LOQ for this data set . range?
*/
PFos t f Pfis Wtrt, in ietta tad stjJralti^.
IC 0 3/!? /<}< }
1 .
000601
i s - I I I
I
t m nvnHMAAHA
I
H
HH
I
SSSSSSSSSSlf SSSSiSSgSSSi 3 3 3 3 3 3 3 3 3 3 Z 3 3 3 3 3 o'ggggSSSSSSSSSgg gggSggggSgSgSggggSSSSgSaS S35SgSK Sggg2SS3?SSS
K E E iS H |8H 3 E 3 3 g = = ||*||3 p i| | a S i||* H IS I ii
S S U iU U s iE E S i l 3 H S H U H i S i I : s 3 i i S p s s s s p i p S S s S S j j p s
ggggggggg
gg
; "" 38855 . S S
g g g g g Sgggggggg
S 8 s s s "sssg'sss'j
i l h h1 1 1 1 1 1 1 1 1 II 3
' 5 5 i i ' ' a ' i . --3 : m S S S S S S S J S S S S S S SSSSii
N O <> O O O
TQ3CX20 R a t L i v a r G roup S an d < A m alia 062491 q u a n t i t a t a d a n d p r l n t a d e n 0 3 /1 7 /9 b y LAC
J.J.H1 14
-- " " '' " " '' - " S S S S S S lS S S ia a s a S R g a S S R S S S R S S S S S S S S S S S S S S S S S G S S S S S S S S S G
8Q u a n t i f y C o m p o u n d t u m u r y B a p e r t
10X120 H at L iv e r G roup 8 and
Saoplo L is t: G :\rata2.9ltt\6a4plaD B \031699b
L a s t M o d ified : Hod Mar 17 13:54:10 1999
M othod:
G :\rata2.P B 0\M ethD B \O 31699b
L o o t a e d i f i o d : Wed Mar 17 0 8 :5 8 :2 0 159
Job Coda:
P rinted:
bod Mar 17 14:3 3 :5 8 1999
Cenpound 2 : f u r r
9 Mimo 1 031699001 2 031699002 3 031699003 4 031699004 5 031699005 6 031699006 7 031699007 8 031699008 9 031699009 10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 15 031699015 16 031699016 17 031699017 18 031699019 19 031699019 20 031699020 21 031699021 22 031699022 23 031699023 24 031699024 25 031699025 26 031699026 27 031699027 28 03169902B 29 031699029 30 031699030 31 031699031
32 031699032 33 031699033
34 031699034 35 031699035 36 031699036 37 031699037 38 031699038 39 031699039
40 031699049 41 031699041 42 031699042 43 031699043 44 031699044 45 031699045 46 031699046 47 031699047 48 031699048 49 031699049 50 031699050
51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067
flaaplaliat la s t MaOH B lank
KeOH B lank 7 o x l2 0 , RBL02229-R20Blk-2, 2 /2 2 /9 9 TOX120, RBL02229-IiivBlk-2, 2 /2 2 /9 9 7oxl20, RBL02229-5ppb-2, 2/22/99 Toxl20, RBL02229-10ppb-2, 2/2 2 /9 9
7oxl20, RBL02229-25ppb-2, 2/2 2 /9 9 Toxl20, *BL02229-50ppb-2, 2/22 /9 9
TOX120, *BL02229-100ppb-2, 2 /2 2 /9 9 10X120, *BL02229-250ppb-2, 2/22/99 T o x l2 0 ,` KBL02229-500ppb-2, 2 /2 2 /9 9 Toxl20, RBL02229-750ppb-2, 2 /2 2 /9 9
TOX120, RBL02229-1000ppb-2, 2 /2 2 /9 9
MeOK B lan k 1OX120, 161F-GS, 2 /2 2 /9 9 , d2S0 . 70X120, 162F-GS, 2 /2 2 /9 9 , 8250 70X120, 163IH35, 2 /2 2 /9 9 , d2S0
70X120, 164F-G5, 2 /2 2 /9 9 , d250 70X120, 165IW35, 2 /2 2 /9 9 , d250 7o x l2 0 , RBL02229-250ppb-CC2, 2 /2 2 /9 9
KaOH B lan k T o x l2 0 , lS r-G 5 , 2/22/99, d2S0 IOX120, 167P-GS, 2 /2 2 /9 9 , d2S0 TOX120, m r - C S , 2 /2 2 /9 9 , d2S0
TOX120, BBM2229-2S0ppb-CC3, 2 /2 2 /9 9 MaOH B lan k TOX120, m r - C i , 2 /2 2 /9 9 , dlOOO TOX120, n a / r - G S , 2/ 22/ 99, d io o o TOXI20, 179r-GC, 2 /2 2 /9 9 , dlOOO T0X120, l a o r - c s , 2/ 22/ 99, diooo TOXI20, OIF-GS, 2 /2 2 /9 9 , dlOOO TOXI20, RBL02229-2SOppfa-CC4, 2 /2 2 /9 9
MaOH B lank TOX120, 182r-GC, 2 /2 2 /9 9 , dlOOO TOX120, 183F-G. 2 /2 2 /9 9 , dlOOO TOX120, ltdF-GC, 2 /2 2 /9 9 , dlOOO TOX120, lOSr-G, 2 /2 2 /9 9 , dlOOO TOX120, 18SF-G6, 2 /2 2 /9 9 , dlOOO TOX120, BBL02229-250ppb-CCS, 2 /2 2 /9 9
MaOH B lank TOX120, 1871-G, 2 /2 2 /9 9 , dlOOO TOX120, 189F-G6, 2 /2 2 /9 9 , dlOOO T o x l2 0 , 190r-G 6, 2 /2 2 /9 9 , dlOOO T o x l2 0 , 191F-G, 2 /2 2 /9 9 , dlOOO
TOX120, RBL02229-250ppb-CC6, 2 /2 2 /9 9
MaOH B lank TOX120. 189r-GS, 2 /2 2 /9 9 TOX120. 170r-G 5, 2 /2 2 /9 9 TO X l20.17lr-G S, 2/22/99 TOX120,' RBl02229-230ppb-CC5, 2 /2 2 /9 9
MaOH B lank TOX120. 1 7 3 M J , 2 /2 2 /9 9 TOX120. 174P-G5, 2 /2 2 /9 9 TOX120. 17C-GS, 2 /2 2 /9 9 TOX120, BBL02229-2S0ppb-CCC, 2 /2 2 /9 9
MaOH B lank TOX120, BBL02229~H20Blk-2, 2 /2 2 /9 9 TOX120, RBX,02229-LlvBlk~2, 2 /2 2 /9 9 Toxl20, BBL02229-Sppb-2, 2/2 2 /9 9 TOX120, RBL02229-10ppb-2, 2 /2 2 /9 9 '
TOX120, BBb02229-2Sppb-2, 2 /2 2 /9 9 TOX120, *BL02229-S0ppb-2, 2 /2 2 /9 9 TOX120, BBL02229-100ppb-2, 2 /2 2 /9 9 TOX120, ABb02229-250ppb-2, 2 /2 2 /9 9 TOX120, BBL02229-500ppb-2, 2 /2 2 /9 9 Toxl20, RBL02229-7S0ppb-2, 2 /2 2 /9 9 TOX120, RBL02229-1000ppb~2, 2 /2 2 /9 9
0 ^ > ix c o iH V i
Vago 2
*VPA naly Analy
A n aly A n aly S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d
A naly naly A naly A n aly A naly A naly
QC A n aly A n aly A n aly A naly
QC A n aly A n aly A n aly A n aly A n aly A naly
QC A naly A naly A n aly
A n aly A n aly A n aly QC A n aly A naly A naly A naly A naly
QC A naly 'A n aly A naly A naly
QC A naly A naly A naly A naly
QC A naly A naly A n aly S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d S ta n d
B td Cono
ST
8.413
1.00
1.00 1.00
1.00 1.00
1.00 1 .0 0 1 .0 0 1.00
9.404 9.420 9.404 9.354 8.413 8.354 8.356 9.354 8.361 8.356
1 .0 0
8.290 6.379 8.453
1.00
8.37*9 6.384 8.384,
8.123
1.00 8.380
7.994
1.00 9.446
1.00
1.00
1.00
1.00 1.00 1.00 1.00 1.00 1 .0 0 1.00 1.00 1.00
9.355
8.418 8.228 8.235 8.294
6.361 8.303 6.301 6.230
8.359 8.359 8.290 9.285 6.269 6.269 8.357 9.295 8.286 8.304 6.292
A rea
614
2274 2907 2500 2199 1931 . 2004 1623 1549 1775
48
12 737
7
1174 9
10
14
982
9
955
741
1213 601 617 809
1236 706 659 623
1229 1222 1139 . 1567 ^ 1317 1190 1278 1211 1102 1179 1136
Beaponae
684.42 2274.17 2906.68 2499.85 2189.27 1931.02 2004.23 1622.84 1548.69 1774.97
47.58
12.14 736.68
7 .0 6
1173.91 8.97 9.59
14.36
. 981.80
9 .1 5
955.41
Cono Tinga tCev
0.41 bb
1.37 bb 1.75 bb 1.51 bb 1.32 bb 1.16 bb 1.21 bb 0.98 bb 0.93 bb 1.07 bb 0.03 bb
-59
37 75 51 32 16 21 -2 -7
7 -97
0.01 bb 0.44 bb 0.00 bb
-99
-56 -100
0.71 bb 0.01 bb 0.01 bb 0.01 bb
0.59 bb
0.01 bb
-29 -99 -99 -99
-41
-99
0.58 bb -42
740.87
1213.32 600.53 616.93 609.04
1235.83 706.35 657.72 623.03
1229.39 1221.94 1136.70 1567.05 1317.43 1169.57 1277.63 1211.23 1102.30 1178.91 1135.66
* %
0.45 bb
0.73 bb 0.36 bb 0.37 bb 0.49 bb
0.74 bb 0.43 bb 0.40 bb 0.38 bb
0.74 bb 0.74 bb 0.69 bb 0.94 bb 0.79 bb 0.72 bb 0.77 bb 0.73 bb 0.66 bb 0.71 bb 0.69 bb
-55
-27 1 -64
-63 -51
-26 -57 -60 -62
-26 -26 -31
-6 -21 -28 -23 -27 -34 -29 -32
B a ta lla 0 6 2 4 9 * q u a n t i t a t a d a n d p r i n t e d o n 0 3 /1 7 /9 9 fey tA C
000603
Q u an tify C a lib ra tio n R ap o rt TCX12 0 1 s t L l v a r G r o u p 5 a n d *
C a lib ra tio n : O :\rata2.pro\C urvuD B\031i99b
L a a t a d l f l a d t Wad Mar 17 1 4 :3 7 :1 0 1*9
P rinted:
Wad Mar 17 1 4 :3 4 :2 9 1999
y^g. ,
t o a l l a 0 4 2 4 9 9 q u a n t i t a t e d a n d p r i n t e d a n 0 3 /1 7 /9 9 b y 1AC
000604
Q n an tify C alib sa tio n X aport TOXI30 K a t U w G roup 5 a n d <
C a llb ra tio n : G:\rm t* 2 .mo\CurvaDX\031(99b
l a a t a o d i f i a d : a d Mas 17 1 4 )3 7 :1 0 19#
P rlntad:
a d Mar 17 14:34:3* 1**9
A m alla 04249* q o a n tlta ta d a n d p s ln ta d o a 0 3 /1 7 /9 9 b y U C
000605
Hacked apere
1 Method t i l e i La*t m odified:
Oi \ r a t a 2 . PRO\KathDB\D31699b.mdb Nad H er 17 0 4 :3 4 :2 0 199
1 Confound Hama: Internal R at.: Q uantity Trace: Cone, o f Standard*:
d o e r RP V a lu es
1 dear Peak Paccar: I n t e g r a t e Missdov f a c t o r s
H09
[None] TIC Cone. X
1.000000, D isabled
1.000000
2 .0 0 0
R eten tio n Tiiae l a i u ) s R e la tiv e R e te n tio n Tima: Time Window (m in * ]:
Peak Selections
] Reepenee Type: Raaponaa:
8 .45, Inablad 0.000. D iaabled 0.717 N earest
S x te z n a l X reaa
1 Polynom ial Types Point o t O rigin: P it W eighting: xia Tran*form ation:
L in ea r
exclude 1/2
Hone
1 Unites
Feak-to-pak am plituda:
293
S m oothing: Window s i a e t i c i a i ) : Humber o f sm ooths:
Smoothing m sthod:
D isablad
3 a
Jo in v a lle y thrashold: Reduce paak ta ilin g : H a is b a a l in e maximum:
] Draw v e r t i c a l th r a a h o ld : D ataet shoulders: Shoulder th raah o ld :
J R elativ h eig h t: A bsoluta height.: R elativ area: A bsoluta area:
30.00 70.00 4.00
10.00 D isablad 10.00
50. 00 Snablad 0.00 D isablad 3.00. B u b led 0.00 D isablad
J
!
000606
Method Report M ethod f i l e s t e s t m o d ified :
Compound: Printed:
a :\rats2 .F R O \X eth D B \Q 3 1 f9 9 b .m d b N ed H er 17 0 < :3 6 ;2 0 1999
2: Surr
Med Mer 17 12:11:29 1999
Coevound Mane: Internal Ret.: Quantify Trace: Cone, of Standards:
Osar RP Value: Osar Peak Factor: Integrate Window Factor:
Surr [Hone] TIC Fixed
1.000000, Disabled 1.000000 2.000
Retention Time (mins): Relative Retention Time: T i M Window (mins) s .
Peak Selection:
S.231, Enabled 0.000, Disabled 0.588
Nearest
Response Typ: Response:
External , ASM*
polynomial Type:
Point of Origin: Pit Weighting: Axis Transformation:
Linear Exclude
Hone None
unit*!
Paak-to-peak amplitude:
77
.Smoothing:
Window aise <e*na): Humber of smooth*: Smoothing method:
. Disabled
3 3
Join valley threshold:
Reduce peek tailing: Raise baseline maximum:
30.00
70.00 4.00
Draw vertical threshold: Detect shoulders: Shoulder threshold:
10.00 Disabled 10.00
Relative height: Abaolute height: Relative area: '
Aheoluta area:
90.00. Enabled
0.00. Disabled 3.00, ttubled 0.00, Disabled
AhL OUW
lAc. mln-hl
000607
Q u a n tify Cengxnmd Sm xaaxy a p a rt TOX120 R a t U v a r O roup 9 a n d
Saapl. U a t i S:\rata2.PHOMaaplaOB\03K99b
L aat aodifiad: ad M ar 17 13:51:10 1999
Hathed:
S:\rata3.m0\MathOB\O31999b3
L aat aodifiad: ad M ar 17 09:59:09 1999
Job Coda:
Frintad:
ad Mar 17 15:01:13 199
" n m m sm
*8. 1
Compound Is FFB8
I Mima 1 031699001 2 031699002 3 031699003 4 031699004 3 031699005 6 031699006 7 031699007 03169900 9 031699009
10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 15 031699015 16 031699016 17 031699017 1 03169901 19 031699019 20 031699020 21 031699021 22 031699022 23 031699023 24 031699024 25 031699025 26 031699026 27 031699027 28 031699028 29 031699029 30 031699030 31 031699031 32 031699032
33 031699033 34 031699034
35 031699035 36 031699036 37 031699037 38 031699038 39 031699039 40 031699040 41 031699041 42 031699042 43 031699043 44 031699044 45 031699045 46 031699046 47 031699047
4 031699049 49 031699049 50 031699050 51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067
G ? iac,
aeplellet Text MtOK Blank KaOH Blank TOX120, RBL02229-B20Blk-2, 2/22/99 Toxl20, RBL02229-ItlvBlk-2, 2/22/99 TOX120, KBL02229-5ppb-2, 2/22/99 TOX120, *BL02229-10ppb-2, 2/22/99 TOX120, RBL02229-25ppb-2, 2/22/99 TOX120, *BL02229-50ppb-2, 2/22/99 TOX120, RBL02229-100ppb-2, 2/22/99 Toxl20, RBL02229-2S0ppb-2, 2/22/99
?oxl20, RBL02229-500ppb-2, 2/22/99 TOX120, RBL02229-*750ppb-2/ 2/22/99' TOX120 RB102229-1000ppb-2, 2/22/99
HaOH Blank TOX120, 161F-G3, 2/22/99, d250
TOX120, 162FH55# 2/22/99, d250 Toxl20, 163F-GS, 2/22/99, d250 TOX120, 164F-GS, 2/22/99, d250 TOX120, 165F-G5, 2/22/99, d250 TOX120, *8102229-250ppb-CC2, 2/22/99
MaOK Blank TOX120, 166F-65, 2/22/99, d250 TOX120, 167F-GS, 2/22/99, d2S0 Toxl20, 168FHS5, 2/22/99, d250 70X120, BBL02229-2S0ppb-CC3, 2/22/99
HaOH Blank to TOX120, 1 7 7 M 6 , 2/22/99, dlOOO toxiio, naXr-G, 2/22/99, diooo Toxl20, 179r-CC, 2/22/99, diooo TOX120, 180F-C, 2/22/99, dlOOO
70X120, 181F-G, 2/22/99, dlOOO 7oxl20, BL02229-2S0ppb-CC4, 2/22/99 KaOH Blank 70X120, IB2F-G8, 2/22/99, dlOOO 10X120, 103F-GS, 2/22/99, dlOOO Toxl20, 1B4F-G6, 2/22/99, dlOOO Toxl20, 185F-G6, 2/22/99, dlOOO Toxl20, 18F-G6, 2/22/99, dlOOO 70X120, BL02229-250ppb-CCS, 2/22/99
HaOH Blank 70X120, 187F-GS, 2/22/99, dlOOO Toxl20, 199F-G6, 2/22/99, dlOOO Toxl20, 190F-G6, 2/22/99, dlOOO Toxl20, 191F-GS, 2/22/99, dlOOO 70X120, RBL02229-2S0ppb-CC6, 2/22/99
HaOH Blank 70X120. 1C9F-G5, 2 /2 2 /9 9 70X120. 170F-GS, 2 /2 2 /9 9 70X120. .171F-GS, 2/22/99 70X120, RBL02229-250ppb-CCS, 2/22/99 HaOH Blank 70X120. 173F-GS, 2/22/99 70X120. 174F-GS, 2/22/99 70X120. 17SF-G3, 2 /22/99 7oxl20, RBL02229-250ppb-CC(, 2/22/99 HaOH Blank 70X120, RBL02229-H20Blk-2, 2/22/99 7oxl20, RBL02229-LivBlk-2, 2/22/99 70X120, RBL02229-Sppb-2, 2/22/99 7oxl20, RBL02229-10ppb-2, 2/22/997oxl20, RBL02229-2Sppb-2, 2/22/99 70X120, BL02229-50ppb-2, 2/22/99 7oxl20, RBL02229-100ppb>2, 2/22/99 70X120, BBLO2229-2S0ppb-2, 2/22/99 7oxl20, RBC02229-500ppb-2, 2/22/99 70X120, BBL02229-7S0ppb-2, 2/22/99
70X120, RBL02229-1000ppb-2, 2/22/99
Typa . Bnaly Bnaly Bnaly Bnaly Stand Stand Stand Stand Stand Stand Stand Stand Stand
Bnaly Bnaly Bnaly Bnaly Bnaly Bnaly
QC Bnaly Bnaly Bnaly Bnaly QC Bnaly Bnaly Bnaly Bnaly Bnaly Bnaly
QC Bnaly Bnaly Bnaly Bnaly Bnaly Bnaly
QC' Bnaly Bnaly Bnaly Bnaly Bnaly
QC Bnaly Bnaly Bnaly Bnaly QC Bnaly Bnaly Bnaly Bnaly
QC Bnaly Bnaly Bnaly Stand Stand Stand Stand Stand 'Stand Stand Stand
Stand
ltd Ceno
XT
5.99 12.00 29.90 59.90 120.00
299.00 599.00 898.00 1198.00
7.878 7.829 7.812 7.828 7.820 7.826 7.830 7.826 7.832
299.00
7.830 7.893
7.849 7.901 7.889
7.850
299.00
7.857
7.793 7.857 7.850
299.00 7.849
299.00 7.851
299.00 299.00 299.00
7.827
7.889 7.766 7.770 7.702
7.831 7.775 7.711 7.765
5.99 12.00 29.90 59.90 120.00 299.00 599.00 98.00 1198.00
7.761 7.757
7.762 7.763 7.766 7.770 7.759
7.775 7.766
Area
52 93 216 325 751 1966 3677 5126 7909
2007 2569 2200 2150 2715 2299
2120 2652 1786 2773
1980
2185
2065
2303 999
1350 1319
234 175 203 1298
46 99 . x 178 328 730 1710 3403 5157 7163
Itaa p o n a
Ceno. Fla. tCav
51.87 92.97 216.31 325.31 750.60 1965.87 3676.73 5125.94 7909.42
2007.43 2569.44 2199.84 2149.83 2714.90 2299.14
2120.11 2651.97 1785.69 *2772.88
5.86 bb 12.91 HM 34.09 MM 52.81 bb '125.83 bb 334.50 bb 628.26 bb 77.10 bb 1355.04 bbX
341.64 MM 438.13 KM 374.67 KM 366.09 MM 463.11 MM 391.72 bb
360.98 MM 452.31 MM 303.56 MM 473.07 MM
-2 8
14 -12
5 12
5 -2 13
31
i 58
1979.57
338.95 bb
13
2184.78
372.08 bb
24
2064.91
2303.09 998.60
1350.47 1319.16
233.79 175.09 202.65 1298.25
351.50 MM
392.40 bb 168.41 bb : 228.83 MM 223.45 bb
37.09 bb 27.01 MM 31.74 bb 219.86 bb
18 *
-25
-2 fi-
46.35 98.73 177.97 328.19 730.44 1709.98 3402.77 5157.15 7163.23
.
4.91 MM 13.90 bb 27.51 MM
53.30 MM
122.37 MM 290.56 MM
581.22 MM 882.46 MH 1226.91 bbX
-18 16 -8
-11 2
-3 -3 -2
2
Cal, Cktck-i fr- * 3 tvtrt, outierj, wUt &}l oHur c-c ixftrc WiUv'V. C-rilcrO. CaL ChicJo will
W u A o - H u r j In d a V a $t,V- Vo v & r ^ uktjlur or n-V
*Vk,j daVa. 3 ac.ttjiVfttile.
H e o iln h j
ftrt ouin. TP Con(V/'c/
ucs 3 -,1o Vtonu. . t f U f c l W W j k
A m a l i a 0 6 2 4 9 8 : q u a n t i t a t a d a n d p r i n t a d e n 0 3 / 1 7 / 9 9 b y LAC
000608
cW rJ
Mi
3,
Q u a n tify CoBpeond S u n eary S taport 10X120 H at l i v a r G roup S and C
8ampia List: G:\rata2.P90\5aqpleDB\O31699b
Lait modifiad: ed far 1? 13:54:10 1999
Mnthod:
G : \ r a t s 2 .PR0\>4athDB\O31699b2
Laat nodlfied: Wed Mar 17 04:39:09 1999
Job Ceda:
Printed:
ad Mar 17 15:01:13 1999
Coapound 2 : Surr
ama 1 031699001 2 031699002 3 031699003 4 031699004 & 031699005 4 031699006 7 031699007 t 031699008 9 031699009 10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 13 031699015 14 031699016 17 031699017 14 031699016 19 031699019 20 031699020 21 031699021
22 031699022 23 031699023 24 031699024
25 031699025 24 031699026 27 031699027 29 031699028
29 031699029 30 031699030 31 031699031
32 031699032 33 031699033 34 031699034 35 031699035 34 031699036 37 031699037
38 031699038
39 031699039 40 031699040
41 031699041 42 031699042 43 031699043 44 031699044 45 031699045
46 031699046 47 031699047 48 031699048
49 031699049 50 031699050
SI 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 *
57 031699057 58 031699058 59 031699059
60 031699060
61 031699061
62 031699062 63 031699063 64 031699064 65 031699065
66 031699066 67 031699067
S a a p l a U a t Tmxt M*OK Blank M.0H Blank Toxl20, RBL02229-H20Blk-2, 2/22/99
Toxl20, ftB1.02229-UTBlk-2, 2/22/99
Ioxl20, m02229 - S p p b - 2 , 2/22/99 TOX120, RBL02229-10ppb-2, 2/22/99
TOX120, BBL02229-25ppb-2, 2/22/99 TOX120, BBt,02229-S0ppb-2. 2/22/99 Toxl20, BBL02229-l00ppb-2, 2/22/99 Toxl20, RBL02229-250ppb-2, 2/22/99
Toxl20,-ULa2229-900ppb-2, 2/22/99 TOX120, BBL02229-7S0ppb-2, 2/22/99 TOX120, BBLG2229-- 1000ppb*>2, 2/22/99 ttaOB Blank 70X120, 1S1F-G3, 2/22/99, d2S0 70X120, 1C2F-GS, 2/22/99, d2S0 ' 70X120, 163F-G5, 2/22/99, d2S0 7OX120, l4r-S, 2/22/99, <1290 70X120, ICSr-GS, 2/22/99, d290
70X120, RBL02229-2S0ppb-CC2, 2/22/99 XaOH Blank 7oxl20, 1SSF-G5, 2/22/99, d230 70X120, 1S7F-6S, 2/22/99, <1230 70X120, 168F-G5, 2/22/99, <1230 70X120, RBL02229-230ppb-CC3, 2/22/99 MaOH Blank A
70X120, 177F-GS, 2/22/99, dlOOO 70x120, m i r - c i , 2/22/99, diooo
TOX120, 179F-G6, 2/22/99, dlOOO 70X120, 180F-G6, 2/22/99, dlOOO 70X120, 181F-G8, 2/22/99, dlOOO 70X120, BBli02229~250ppb-- CC4, 2/22/99 HaOH Blank 70X120, 182F-G8, 2/22/99, dlOOO 70X120, 183F-G6, 2/22/99, dlOOO TOX120, 194F-G4, 2/22/99, dlOOO 70X120, 183F-G, 2/22/99, dlOOO
TOX120, 188F-64, 2/22/99, dlOOO Toxl20, RBl02229-250ppb-CC5, 2/22/99 KaOK Blank 70X120, 187F-GS, 2/22/99, dlOOO 70X120, 189F-G6, 2/22/99, dlOOO 70X120, 190r-G6, 2/22/99, dlOOO 70X120, 191F-G8, 2/22/99, dlOOO 70X120, RBU32229-250ppb-CC6, 2/22/99 KaOB Blank 70X120. 169F-GS, 2/22/99 70X120. 1 7 0 M S , 2/22/99 70X120. 171F-G5, 2/22/99 70x120, BBL02229-230ppb-CCS, 2/22/99 HtOU Blank
70X120. 173F-GS, 2/22/99 70x120. 174F-GS, 2/22/99 7oxl20. 17SI^GS, 2/22/99 70X120, RBU)2229-250ppb-CC<, 2/22/99 HaOH Blank 7oxl20, m02229-H2OBlk-2, 2/22/99 70X120, BBt.02229-LiaBlk-2, 2/22/99 7oxl20, RBL02229-5ppb-2, 2/22/99 70X120, RBU)2229-10ppb-2, 2/22/99 7oxl20, BBL02229-2Sppb-2, 2/22/99 7oxl20, BBli02229-30ppb*2, 2/22/99 70X120, BBl*02229-100ppb*2, 2/22/99 7oxl20, BB1.02229-23appb-2, 2/22/99 7oxl20, BB02229-500ppb-2, 2/22/99 70X120, Bt.02229-7S0ppb-2, 2/22/99
70X120, BB1.02229-10aOppb-2, 2/22/99
(f > @ IA C 03
Typo taaly Bnaly Analy Bnaly Stand Stand Stand Stand Stand Stand Stand Stand Stand Bnaly Bnaly Bnaly Bnaly Bnaly' Bnaly
OC Bnaly Bnaly Bnaly
Bonca l y
Bnaly Bnaly Bnaly Bnaly Bnaly Bnaly
OC Bnaly Bnaly
Bnaly Bnaly Bnaly
Bnaly
. oc
Bnaly Bnaly Bnaly Bnaly Bnaly.
oc
Bnaly Bnaly Bnaly Bnaly
OC - Bnaly
Bnaly Bnaly Bnaly
OC Bnaly Bnaly Bnaly
Stand .Stand Stand Stand stand Stand Stand Stand Stand .
Std Cena
xr
1.00 1.00
1.00 1.00 1.00 1.00
1.00 1.00 1.00
1.412 8.482
8.404 8.420 8.404 8.354 8.413
8.354 8.358
8.354 8.361 6.356
1.00
8.290 6.378 8.453
1.00
8.379 6.364 8.384
6.123
1.00 8.380
7.9B4
1.00 8.446
1.00 8.355
* 8.418
8.228 6.235 1.00 8.294
1.00
8.361 8.303 8.301 8.230
1.00 1.00 1.00
1.00 1.00 1.00
1.00 1.00 1.00
8.359 6.359 8.290 8.285 8.269 8.289 8.357 8.295 6.286 6.304 8.292
Area
684 904 2274 2907 2500 2189 1931 2004 1623 1549 1775
48
12 737
7
1174 9
10
14
982
9
955
.
741
1213 601 617 809
1236 706 658 623
1229 1222 1139 1567 1317 1190 1278 1211 1102 1179 1136
Aeapona
684.42 903.69 2274.17 2906.68 2499.85 2189.27 1931.02 2004.23 . 1622.84 1548.68 1774.97
47.56
12.14 736.68
7.06
* 1173.91 8.97 9.59
14.36
981.80
9.15
955.41
Cono. Fina. .Dm
0.41 bb 0.54 bb
1.37 bb
1.75 bb 1.51 bb
1.32 bb 1.16 bb 1.21 bb 0.96 bb
0.93 bb 1.07 bb
0.03 bb
-59 -46
37 75
SI
32 16
21 -2 -7
7 -97
0.01 bb
0.44 bb 0.00 bb
-99 -56
-100
0.71 bb 0.01 bb 0.01 bb
0.01 bb
-29 -99 -99
-99
0.59 bb - -41
0.01 bb
-99
0.58 bb -42
740.97
0.45 bb
1213.32 600.53 616.93 809.04
1235.83 706.35
- 657.72 623.03
: *i *
0.73 bb 0.36 bb 0.37 bb 0.49 bb
0.74 bb 0.43 bb 0.40 bb 0.38 bb
1229.39 1221.94
1138.70 1567.05 . 1317.43 1189.57
1277.63 1211.23 1102.30
1178.81 1135.66
0.74 bb 0.74 bb
0.69 bb
0.94 bb 0.79 bb
0.72 bb 0.77 bb 0.73 bb
0.66 bb 0.71 bb 0.68 bb
-55
-27 -64
-63 -SI
-26 -57 -60 -62
-26 -26 -31
-6 -21 -28 -23 -27 -34 -29 -32
A m alia 0 4 2 4 9 9 : q u m n tita ta d a n d p r i n t a d m i 0 3 /1 7 /9 9 k y IAC
000609
Q u an tify C a lib ra tio n K ap ert 100(120 R a t L i v a r G ro u p 9 a n d (
Calibratieni a : \ r a t a 2 . P m \CurmDB\03M99b2
Last modified: Wed Mar IT 19:09:90 1**9
Printed:
Wad Ma r 17 19:01:49 199*
Pag 1
000610
Q u a n tify C a lib ra tio n R ap o rt TQX120 R a t L l v a r C ro u p S a n d 3
Calibration: a:\rata2.3RO\CurraOB\031l93ba
Laat nodifiad: Wad Mar 17 18:04:30 1333
prlntad:
Wad Mar 17 13:01:43 !
fW 2
I n a i l a 062498: q uant i t a ra d and p r ln ta d on 0 3 /1 7 /3 3 b y M C
000611
1 Method Report
Method file:
1 Last modified: Compound:
printed:
O:\rats2.FRO\MethDS\031699b2.ndb Wa d Mar 17 06:39:09 1999
1: FFHS W ad Mar 17 1 3 : U : 3 S 1999
1 Compound Mane: Internal Ref.: Quantify Trace: Cone, of Standards:
u*c r f valuei
I User Pm I Factor! Integrate Window Factor t
**** , ,n
3 9 9 . 0 0 00.00 Cone. *
1.000000, Disabled 1.000000 2.000
tetnfcioa T i M (lint) i
1 Relative Retention Tim*: T i M Window (mine) t
Peak Selection:
7.543, Xnablod 0.000# Disabled 0.020
XMTMt
Response Typo i
] Response:
eternal Arons
polynomial Typ*
1 Point of Origin: Pit weighting: Axia Transformation:
Exclude 1/X
1 units: Peak-to-peak amplitude:
Window eixe (scans): Number of smooths: Smoothing method;
293
Dlaablad J *
J oin valley fehraahoid: * Jtadoca peak tailing: Raise baseline auxiaum:
30.00 70.00 4.00
Draw vortical threshold: Datact shoulders:
Shoulder threshold:
/
10.00 Disabled 10.00
J Relative height! Absolute height:
Relative are.: Rbaolute area:
50.00, Snabled 0.00, Disabled 3.00, Enabled
0.00, Disabled
!
Artekd 0LW8________/AC Qllfrlw
j:
I 000612
M ethod R e p o rt
H ath o d f i l e : L u t B O d lfiad : Confound:
P rin te d :
O :\r.ta2 .F R O \H eth L B \0 3 1 i9 9 b 2 .sd b Wad W ar 17 0 i:5 S :0 J 1599
2 : Surr
Wed M u 1 7 1 3 :1 1 :3 3 1991
Conpouad Naaei Internal Ret.t Quantity Trace: Cone, of 3tenderda:
Bear RF Value: D a n Peek Factor: Integrate Window Factors
Sun (Hone) TIC Fined
1.000000, Disabled 1.000000 2.000
Retention Tine (mine) i Relative Retention Tine: Tine Window (nine) t
Peek Selection:
9.331, Rnabled 0.000, Disabled
o.saa
Nearest
Response Type: Responses
Brterael Areas
Polynomial Types Point of Origin:
Fit Weighting: Axis Transformations
Linear Ktclude Hon
Hone
Units;
Feak-to-peak amplitude:
Smoothing: Window site (scans): Humber of smoothes Smoothing methods
77
Disabled 3 2
Join valley thresholds `
Reduce peak tailings Raise baseline maximum:
30.00 70.00 4.00
Draw vertical threshold; Detect ehouldere: Shoulder thresholds
10.00
Disabled 10.00
Relative heights Absolute heights
Relative areas . Absolute areas
SO .00,, Bnahled . O.OOg Disabled
3.00. enabled 0.00. Disabled
A tJ ia . o m w ____ AC__
000613
FACT: DATA REVIEW SUMMARY FORM
Date: * /s h i
000614
Q u an tify Conpound Susaaxy R eport Tox-120 R a t U v u
Raspi* U s t i M:\rata2.PRO\aasplaDa\04209Ra
Last nodlflad: Thu Apr 22 09:02:11 1999
Method:
H:\rats2.9R0\ttethDB\042099o
Last nodlflad: Tu* May 04 08:52:41 1999
Job Cod*:
Printed:
Hod May OS 11:10:51 1999
BESFcopyAMliABIE
Pag* 1
Compound 1: PQAA
Sanplallat Taxt
Typ
Std Conn
XT
Arra
Xaapona Cene. llaga 4Dv
042099002
MsOM Blank
042099003 042099004 042099005 04209900 042099007
042099008 042099009
042099010 10 042099011 11 042099012
12 042099013
TOX120, RBL04199-H20Blk-l, 4/19/99 TOX120, RBL04199-UTBlk-l, 4/19/99
Toxl20, RBL041S9*3ppb-l. 4/19/99 TOX120. RBL04199-10ppb-l, 4/19/99 TOX120, RBL04199-25ppb-l, 4/19/99 TOX120, RaL04199-50ppb-l. 4/19/99 TOX120, ABL04199-100ppb-l, 4/19/99
TOX120, RBL04199-250ppb>l, 4/19/99 Toxl20, BBL04199-S00ppb-1, 4/19/99 Toxl20, RBL04199-750ppb-l, 4/19/99
TOX120, RBL04199-1000ppb-l. 4/19/99
Standard Standard Standard . Standard Standard
Standard Standard Standard . Standard
5.56
11.10 27.80
55.60 111.00 278.00 556.00 833.00
1111.00
4.483
4.480 4.477 4.479 4.479 4.486 4.484 4.481 4.484
74
37 77 121 16S 374 140 922 107S
73.837
57.350 76.531 121.096 165.361 374.157 739.533 921.621 1074.679
32.81 MM .
20.70 HM 34.85 MM 7.75 MM 100.42 bb 254.53 bb
524.21 MM <58.0 bbX
771.72 bbX
86 25
22 -10
-8 -6 -21 -31
13 042099014
HOH Blank
14 042099015
Toxl20, RTL, IM, Gl, 4/19/99
15 042099016 16 042099017
OX120, RTL, 2M, Gl, 4/19/99 ToxllO, RTL, 3M, Gl, 4/19/99
17 042099018
TOXI20, RTL, 4M, Gl, 4/19/99
16 042099019 19 042099020
TOXI20, RTL, SH, Gl, 4/19/99 T0XI20, RBL04199-250ppb~CCl, 4/19/99
QC
278.00 4.483
355 354.906 240.32 bb
20 042099021
HaOH Blank
21 042099022
T0XI2O, RTL, M, Gl, 4/19/99
22 042099023
TOXI20, RTL, TM, 61. 4/19/99
23 042099024
TOXI20, RTL, SM, Gl, 4/19/99
24 042099025
TOXI20, RTL, 9M, Gl, 4/19/99
25 042099026 26 042099027
TOXI20, RTL, 10H, Gl, 4/19/99 T0XI2O, RBL04199-250ppb-CC2, 4/19/99
OC
278.00 4.480
447 447.316 308.53 bb 11
27 042099028 28 042099029
MaOH Blank T0XI2O, RTL, U M , Gl, 4/19/99
29 042099030
T0XI2 O, RTL, IBM, Gl, 4/19/99
30 042099031
T0XI2O, RTL, 14M, Gl, 4/19/99
31 042099032 32 042099033
T0XI2O, RTL, 15M, Gl, 4/19/99 T0XI2O, RBL04199-250ppb-CCl, 4/19/99
oc
278.00 4.484
341 341.490 230.42 MH
33 042099034
MaOH Blank
34 04209903S
TOXI20, RTL, 17K, G2, 4/19/99
35 042099036
TOXI20, RTL, 18M, G2, 4/19/99
36 042099037
TOX120, RTL, 19M, G2, 4/19/99
37 042099038
TOXI20, RTL, 2OM, G2, 4/19/99
38 042099039
TOXI20, RTL, 21M, G2, 4/19/99
39 042099040
TOXI20, RBL04199-2J0ppb-CC4, 4/19/99
40 042099041
MaOH Blank
41 042099042
TOX120, RTL, 22M, G2, 4/19/99
42 042099043 T0XI2 O, RTL, 23M, G2, 4/19/99
43 042099044
TOXI20, RTL, 24M, 62, 4/19/99
44 042099045
TOXI20, RTL, 2 SM, G2, 4/19/99
45 042099046
TOXI20, RTL. 2 SM, G2, 4/19/99
46 042099047
TOXI20, RBL04199-2S0pph-CC5, 4/19/99
QC QC
278.00
4*480 4.482 4.480 4.471
4.495 4.47
3899 5143 5371 5320 5764
413
4.479
4423
4.479
3922
4.487
5424
4.485 2795
4.484
3068
278.00 4.479
343
3898.674 5142.572
5371.353 5319.678 5764.340
413.054
2855.93 bb
3774.03 bb 3942.89 bb 3904.90 bb
4232.95 bb 283.24 bb
4422.820 3922.058
5424.250 2794.636 3067.762
342.693
3242.79 bb 2873.19 bb 3981.94 bb 2041.05 bb 2242.64 bb
231.30 MH
fO-to, I ' I S
d ilu W
47 042099048
MaOH Blank
4*8 042099049
TOXI20, RTL, 27M, 62, 4/19/99
49 042099050
T0XI2O, RTL, 29M, 62, 4/19/99
J 50 042099051 51 042099052 52 042099053
53 042099054 54 042099055
TX120, RTL, 3OM, G2, 4/19/99 T0XI20, RTL, 31M, 62, 4/19/99
TOXI20, RBL04199-250ppb-CC, 4/19/99
MaOH Blank T0XI2O, RTL, 33M, 63, 4/19/99
QC
55 042099056
T0XI2 O/ RTL, 34M, 63, 4/19/99
56 042099057
TOXI29, RTL, 3SM, G3, 4/19/99
57 042099058
TOXI20, RTL, 36M.'63, 4/19/99
58 042099059
TOXI20, RTL, 37M, G3, 4/19/99
59 042099060
TOXI20, RBL04199-250ppb-CC7, 4/19/99
QC
278.00 278.00
4.484 4.483 4.479 4.479 4.483
4.481 4.479 4.479 4.495 4.453 4.495
2119 1448 1545 1644
419
4232 5668 4583 4657 5081
411
2119.342 1448.030 1544.621
1644.361
418.663
1542.63 bb 1047.14 bb
1118.43 bb 1192.05 bb
287.38 bb
4232.225 5668.119 4582.970
4657.182 5081.245
411.447
3102.12 bb 4161.93 bb 3361.00 bb 3415.77 bb 3728.77 bb
282.05 MM
^ bitA l*lo
U
60 042099061 61 042099062 62 042099063 63 042099064
64 042099065 65 042099066 66 042099067
MaOH Blank 4 TOXI20, RTL, 38M, 63, 4/19/99
TOX120, RTL, 39M, 63. 4/19/99 T0XI2O, RTL, 4OM, 63, 4/19/99
' TOX120, RTL. 41M, G3, 4/19/99 TOX120', RTL, 42M, 63, 4/19/99 TOXI20, RBL04199-250ppb-CC8, 4/19/99
QC
278.00
4.472 .4*483
4.475 4.488 4.481 4.480
5831 4587'
5604 . 3410
1319 443
5631.017
4587.398 5603.629 3409.806 1318.606
442.641
4134.55 MM 3364.27 IM
4114.33 bb 2495.10 bb
951.61 bb 305.07 bb
67 042099068 68 042099069 69 042099070 70 042099071 71 042099072 `72 042099073
MaOH Blank T0XI2O. RTL, 43M, 63, 4/19/99 TOXI20, RTL, 45H, 63, 4/19/99
TOX120, RTL, 4EM, G3, 4/19/99 TOXI20, RTL, 47M, G3, 4/19/99
TOXI20, RBL04199-250ppb-CC9, 4/19/99
QC
278.00
4.483 4.479 4.480 4.486 4.484
2595 1076
650
1384 398
2594.520 1075.927
850.322 1383.690
398.107
1893.35 bb 772.50 bb 605.99 bb
999.65 bb 272.20 MM
73 042099074
MaOH Blank
74 04209907
TOX120, RTL, 5M, 05, 4/19/99
75 04209907
TOX120, RTL. 6M. 05, 4/19/99
7 042099077
TOX120, RTL, 73H, 05, 4/19/99 '
77 04209907
TOX120, RTL. 74M, 05, 4/19/99
78 042099079
79 042099080 80 042099081
TOX120, RTL, 7SM, GS,
4/19/99-
TOX120, R9L04199-250ppb-CC4, 4/19/99
MaOH Blank
OC
278.00 4.454
413 413.360 283.48 MM
81 042099082
TOX120. RTL, 77H, 05, 4/19/99
82 042099083
TOX120, RTL, 78M, GS, 4/19/99
83 042099084 84 042099085
Toxl20, RTL, 79M, OS, 4/19/99 TOX120, RBL04l99-2S0ppb-CCS, 4/19/99
QC
278.00 4.459
364 363.646 246.77 W
85 04209908 86 042099087 87 042099088
88 042099089
MaOH Blank TOX120, RTL, 3M, Gl-2S0ppb-H3, 4/19/99 Toxl20, RTL, 3H. Gl-2S0ppb-MSD, 4/19/99
TOX120, RTL, CM. Ql-2S0ppb-MS, 4/19/99
4.477 4.472
4.485
372 372.063 252.98 bb 380 360.105 258.92 MM 385 384.795 262.38 bb
,m ad elin * 041099: q u a n tita ta d and p r ln ta d by i 5 /0 9 /9 9
000615
1 1 !
]
Quantify Compound Summary Raport Tox-120 Rat U v
Saapla U a t : N:\zata2.PRO\8amplaDB\04209Sa last modlflads Thu Apr 22 09:02:11 1999
Hathod:
H:\rata2.PRO\MathDB\042099a
Last modlflad: Tua Hay 04 09:52:4 1999
Job Coda:
Printad:
ad Hay 05 11:10:51 1999
Compound 1: PGAA
1 Mu m 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 96 04209909*7 9*7 042099099 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 04209910S
Samplallat Tax
Typ
fltd Cono
TSX120, RTL, 6H( Gl-2S0ppb-M3D. 4/19/55
T0X120, RTL, 13H, 61-250ppb-HS, 4/19/99
TOX120, RTL, 13H, Gl-2iOppb-HSO, 4/19/99
Toxl20, RBL04199-250ppb-CC6, 4/19/99
OC
278.00
H*OK Blank
ToxllO, RBL04199-H20BU-1, 4/19/99
Toxl20, R8L04199-LlvBlk-l, 4/19/99 TOX120, RBL04199-Sppb-l, 4/19/99 Toxl2( RBL04199-10ppb-l, 4/19/99
Toxl20, RBL04199-25ppb-l, 4/19/99 Toxl-20, RBL04199-50ppb-l, 4/19/99 TOX120, RBL04199*100ppb-l, 4/19/99 TOX120, RBL04199-250ppb-l, 4/19/99 TOX120, R8L04199-S00ppb-1, 4/19/99 TOX120, RBL04199-750ppb-l, 4/19/99 Toxl20, RBL04199-1000ppb-l,'4/19/99
Standard Standard Standard Standard . Standard
Standard Standard Standard Standard
5.5
11.10 27.80 55.60 111.00
278.00 556.00 833.00 1111.00
XT 4.483 4.484 4.486 4.475
4.485 4.479 4.484 4.483 4.483 4.484 4.470 4.485 4.484 4.441
hgt 2
Aru 397 492 449 377
110 18 57 89
107 173 381 847 1080 1247
Xaipon 397.088
492.434 449.098 376.897
Cone. Flogs %Dev
271.45 bb
341.83 bb
309.84 bb
256.55 MM
-8
110.250 17.853 56.918 8.793
107.191 172.713 380.632 647.164 1080.222 1247.489
59.74 bb 0.00 MM
20.38 bb
43.90 MM 57.48 bb 105.84 bb 259.31 bb 603.65 MM 775.67 MMX 899.12 bbX
-100 84 56 3 -5 -7
9 -7
-19
j
1
] !
i
r
m ad alln a 041099: :q u a n tlta ta d and p r in ta d by m aa 5 /0 5 /9 9 i
is
000616
I
g ssssassssa ssaass sssssn sascs ssgasa ssssss aaass ssssss ssasss assss ssaasa sssssass
s i i s ia - " - s a l a s s a i i i i H s s s s r s g a s s a i i i i i i s a s a s s
i v i ss.ssg sg asa a s s s S t r i s i
I ssssasssa saageS ssaasii salsa aaaali sass-s sasis sasssa sassss assss ssSsss sSsalsas 3HSSS5Sg H H H P 'i i i2 | 333SS MU U U U t t i i SSISss alias issasi S5S58|s |
J SHSaasSsas S58gg ffil iliS a SaSSI saassl salsa 4SS3!'E>333g SSSSg S3g33g &g' ggg
s ssssaassss saassa sSaass sassi sasasa aassia ssaas saasss aiassa sassi sassi? aasssaas
l 52S8SSSSS
s ss
i 5
s s s g s ss
m
N
b illi
[lp (afa [sfa'
]s
s ^s
m
S s3
a
s
a
s
1
2
\ li..I|
1filai
sIg
s
-J'T .
s- m i r
| S3SS;
s'ssss .s8s8'8s'8s8sSs s3'3s'3s3's3's1's 3`8'88'f 833*33 s's's'ss"^ 33331 SSBBsf 3SS5. ggg
i --ssssssti m Y ii m t i l * * & i i i i i m M i i m i i U m i i i i i t t i t t m m i m
> g g o g g g g g g g S o 0*0=o a o'o'o 0 =0800 o o o 3 00 0*000 a 00 00 00 a 00000S g o'o'o'o oSoo'o'o o o 3 g-g'g'g'g-agg'g'gg'g a gg-g-g-a g gg '
pgwgggo^NM^*nr^a>OjjjNrn
inv^wre
[U H I!
l i s ils iiiis s iiis s s s s s s s s s s is s s is is is s s sIissHiiSss s; is s s s s is iiiis s lis s s s lillig s s ls s s s llis ii; wr--sasnsssasssasasasRsssasasasKssgasaasaaaasaaasasKsssassasscsssaspsssssssasssssss
Q u a n t if y C om pound Suom iary R a p o r t Tox-120 R at U v a r
Sampla Liat: H:\rata2.PR0\SamplaDB\042099o
l u t modifiad: Thu Apr 22 09:02:11 1999
Mathod:
M:\rata2.P9O\MathDB\042099c
Laat modifiad: Tua May 04 09:92:49 1999
Job Coda:
Printad:
Wad May 09 11:10:91 1999
BESTCOPY AVAILABLE
Pacja 4
Compound 2: 9109
9 Mama 09 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 96 042099097 97 042099099 90 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105
Samplaliat Text
*
Toxl20, m , 6M. Gl-2S0ppb-MSD, 4/19/99
Std Cena
TOX120, RTL. 13M. Gl-2S0ppb-M3, 4/19/99
TOX120, RT1, 13M, Gl-250ppb-H3D, 4/19/99
TOX120, RBL04199-250ppb-CC6, 4/19/99
QC
279.00
MaOH Blank Toxl20, RBL04199-H2OBU-1, 4/19/99
Toxl20, RBL04199-UvBlk-l, 4/19/99 Toxl20, RBL04199-5ppb-l, 4/19/99 Toxl20, BBL04199-10ppb-l, 4/19/99 Toxl20, RBL04199-25ppb-l, 4/19/99 TOX120, RBL04199-S0ppb-1, 4/19/99 Toxl20, RBL04199-100ppb-l, 4/19/99
ToxI20. *BL04l99-250ppb-l, 4/19/99 TOX120, RBL04199-S00ppb-1, 4/19/99 TOX20, RBL04199--750ppb~l* 4/19/99
TOX120, RBL04199-1000ppb-l'.' 4/19/99
Standard Standard Standard Standard Standard Standard Standard Standard Standard
5.56
11.10 27.80 55.60 111.00 278.00
556.00 033.00 1111.00-
RT 4.622 4.779 4.760 4.616
4.924 4.760 4.619 4.760 4.776 4.779 4.776 4.906 4.779 4.779 4.760
Arsa 2997 2906 2923 2396
57 139 164 199 382 637 1056 2337 4350 6492 7651
Raapanaa 2987.061 2905.830 2922.907 2396.152
56.592 139.347 163.940 198.888 392.141 637.350 1058.178 2336.510 4349.988 6481.772 7650.592
Cone, flag 390.48 bb 379.43 bb 381.75 bb 310.07 bb
IDV
*\ Y )
.
)bnj w ' ffwjfwfvifyJs
i6
12 O S I oi/
* 0.00 bb 2.98 bb 6.32 bb
li:08 bb
36.01 bb 70.74 bb 128.01 bb 301.96 bb 575.94 MM 66.03 bbX 1025.08 bbX
14 0
30 27
15 9
4 4 -8
I*
m ad a lin a 041099: :q u a n titn ta d an d p r in ta d b y m aa 5 /0 5 /9 9
000618
Q u an tify Conpound Sum uzy R apert Tex-130 R at L iv .r
Sanpla U a t : H;\raU2.mo\SaiqplaDB\a4309Se
Last audifiad: Thu Apr 2 2 09:03:11 1999
Mathod:
H:\rata2.PRO\MathDB\042099o
Laut modifiad: Tua Hay 04 09:53:4 1999
Jab Coda:
Printad;
Wad May OS 11:10:51 1999
BESTCOPYAVAIUBi!
Paa 5
Compound 3s PFHfl
Mama 1 042099002 2 042099003 3 042099004 4 042099005 5 042099006 6 042099007 7 042099008 8 042099009 9 042099010 10 042099011 11 042099012 12 042099013 13 042099014 14 042099015 15 042099016 16 042099017 17 042099018
18 042099019 19 042099020
20 042099021
21 042099022 22 042099023 23 042099024 24 042099025 25 042099026 26 042099027 27 042099028 28 042099029 29 042099030 30 042099031 31 042099032 32 042099033 33 042099034 34 042099035 35 042099036 36 042099037 37 042099038
38 042099039 39 042099040 40 042099041
41 042099042 42 042099043 43 042099044 44 042099045 45 042099046 46 042099047 47 042099048
48 042099049
49 042099050 50 042099051
51 042099052 52 042099053 S3 042099054
54 042099055 55 042099056 56 04209905757 042099058
58 042099059 59 042099060 <0 042099061 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 66 042099067
67 042099068
68 042099069 69 042099070 70 042099071 71 042099072 72 042099073 73 042099074 74 042099075 75 042099076 7 6 .04309907? 77 042099079
78 042099079
79 042099080
80 042099081
81 042099062
82 042099083
83 042099084
84 042099085
85 042099086 86 04209908?
67 042099088 88 042099089
Smplallit Taxt MaOH Blank
TOX120, RSL04299-X2O81k-l, 4/19/99 TOX120, RBL04199-L1VBU-1, 4/19/99 TOX120, RBL04199-Sppb-1, 4/19/99
TOX120, RBL04199-10ppb-l, 4/19/99 TOX120, RBL04199-25ppb-1, 4/19/99 TOX120, RSL04199~50ppb-l, 4/19/99 TOX120, RBL04199-100ppb-l, 4/19/99 TOX120, RBL04199-250ppb-l, 4/19/99 T0X12, RBL04199-S00ppb-1, 4/19/99 Toxl'20, RBL04199-750ppb-l, 4/19/99 TOX120, RBL04199-1000ppb-l, 4/19/99 MaOH Blank ToxlZO, RTL, 1M, Gl, 4/19/99 TOX120, RTL, 2M, Gl, 4/19/99 TOX120, RTL, 3M, Gl, 4/19/99 TOX120, RTL, 4M, Gl, 4/19/99 Toxl20, RTL, 5M. Gl, 4/19/99 TOX120, RBL04199-2S0ppb-CCl, 4/19/99 MaOH Blank TOX120, RTL, CM# Gl, 4/19/99 Toxl20, RTL, 7M, Gl, 4/19/99 TOX120, RTL, 8M, Gl, 4/19/99 TOX120, RTL, 9M, Gl, 4/19/99 Toxl20, RTL, 10M, Gl, 4/19/99 Toxl20, RBL04199-250ppb-CC2, 4/19/99 MaOH Blank TOX120, RTL, 11M, Gl, 4/19/99
TOX120, RTL, 13M, Gl, 4/19/99 . TOX120, RTL, 14M, Gl, 4/19/99
TOX120, RTL, 1SH, Gl, 4/19/99 Toxl20, RBL04199-250ppb-CC3, 4/19/99
MaOH Blank TOX120, RTL, 17M, G2, 4/19/99 TOX120, RTL, IBM, G2, 4/19/99 Toxl20, RTL, 19M, G2, 4/19/99 TOX120, RTL, 2H, G2, 4/19/99 TOX120, RTL, 21M, G2, 4/19/99 TOX120, RBL04199-250ppb-CC4, 4/19/99 MaOH Blank TOX120, RTL, 22M, G2, 4/19/99 TX120, RTL, 23M, G2, 4/19/99 TOX120, RTL, 24M, G2, 4/19/99 TOX120, RTL, 25M, G2, 4/19/99 Toxl20, RTL, 26M, G2, 4/19/99 Toxl20, RBL04199-250ppb-CC5, 4/19/99 MaOH Blank ToxlZO, RTL, 27M, G2, 4/19/99 Toxl20, RTL, 29M, G2, 4/19/99 TOX120, RTL, 30M, G2, 4/19/99 TOX120, RTL, 31M, G2, 4/19/99 TOX120, RBL04199-25Oppb-CC6, 4/19/99
MaOH Blank TOX120, RTL, 33M, G3, 4/19/99 Toxl20,,RTL, 34M, G3, 4/19/99 TOX120, RTL, 35H, G3, 4/19/99 TOX12, RTL, 36H, G3, 4/19/99 Toxl20, RTL, 37M, G3, 4/19/99 TOX120, RBL04199-250ppb-CC7, 4/19/99
MaOH Blank TOX120, RTL, 38M, 63, 4/19/99 TOX120, RTL, 39M, G3, 4/19/99 TOX120, RTL, 40M, G3, 4/19/99 TOX120, RTL, 41H, G3, 4/19/99* Toxl20,* RTL, 42M, G3, 4/19/99 TOX120, RBL04199-250ppb-CC8, 4/19/99
MaOH Blank TOX120, RTL, 43M, G3, 4/19/99 Toxl20, RTL, 45M, G3, 4/19/99 . TOX120, RTL, 46M, G3, 4/19/99 TOX120, RTL, 47M, G3, 4/19/99 * TOX120, RBL04199-250ppb-CC9, 4/19/99 MaOH Blank TOX120, RTL, 65M, GS, 4/19/99 TOX120, RTL, 66M, GS, 4/X9/99 ToxlSO RTL, 73M, GS, 4/29/99 Toxl20, RTL, 7 4M 65* 4/19/99
TOX120, RTL, 7SM, 65, 4/19/99 Toxl20, RBL04l99-250ppb-CC4, 4/19/99 MOH Blank Toxl20, RTL, 77M G5, 4/19/99 TOX120, RTL, 78M, G5, 4/19/99 Toxl20, RTL, 79M, GS, 4/19/99 TOX120, RBL04199-2S0ppb-CCS, 4/19/99 * M0H Blank TOX120, RTL, 3M, 61-230ppb-MS, 4/19/99 Toxl20, RTL, 3M, Gl-250ppb-MSD, 4/19/99 Toxl20, RTL, 6M, Gl-250ppb-MS, 4/19/99
Typ Standard Standard Standard Standard Standard Standard Standard Standard Standard
QC
QC
QC
QC
QC
QC
QC
QC
QC
QC
QC
Btd Cono
RT
Aram
Rasponaa Cono. Flaga %Oav
5.56
11.10 27.80 55*60 111.00 278.00 556.00 833.00 1111.00
4.160 4.158
4.154 4.112 4.156 4.118 4.117
4.114 4.117
278.00
4.162 4.155
4.161 4.154 4.118
4.116
278.00
278.00
4.161 4.159 4.115 4.160 4.158 4.112
4.160 4.162 4.118 4.159 4.118
99
179 502 895 1993 4901 10347
1516616551
99.365 179.476 502.222
895.168 1992.760
4900.550 ' 10347.100
15166.480 16551.121
1325 1876
965 . 995
607
5128
1324.885
1875.834 964.554 995.322 606.564
5127.719
966
792 823 854 * 4262 4828
965.683
792.578 823.111 853.907
4261.949 4828.117
2338 2072
1765 1326 5008
2337.712
2072.375 1764.636
1326.162 5008.266
4.87 HM
9.01 MM 25.67 MK 45.96 MM 102.63 MM 252.77 bb
534.00 bb 782.84 bb 854.34 MMX
68.15 KM 96.59 MM
49.54 MM 51.13 KM 31.06 MM 264.50 bb
'
49.60 bb 40.61 bb
42.24 MM 43.83 MM
219.80 bb 249.03 bb
-12 -19
-8 -17
-8 -9 -4 -6 -23
\\\\-
-10
120.44 bb
106.74 MM 90.8S bb 68.21 bb
258.33 bb
-7
278.00 278.00
4.157 4.159 . 4.157 4.148 4.117 4.153
4.1SS 4.156 4.120 4.118 4.161 4.112
703 886 1144 2148 495 5496
562 ' 629 584 721 331 4659
702.749 885.923 1143.722 2147.549 494.722 5495.959
562.068 628.042 583.798 721.418 330.691 4658.773
36.02 MM 45.48 bb 58.79 bb 110.62 bb 25.28 bb 283.52 bb
28.76 bb 32.21 bb 29.86 bb 36.99 bb 16.81 MM 240.29 bb
/ /
\c
2)
I
\\
-14
278.00
4.117
4.160 4.156 4.111 4.118
445 667
863 410 5427
* 445.452
666.912 862.746 . 409.525 5426.568
22.74 bb 34.17 bb
44.29 bb" 20.88 MM 279.93 bb
.
1
278.00
4.158 4.157
4.113 4.131 4.133 4.130
1268 1021 1716
884 1278 4754*
1268.351 1021.077 1715.671
883.895 1278.325 4754.072
85.23 MM 52.46 MM 88.33 bb 45.38 bb
65.74 bb 245.21 MM
-12
278.00
4.150 4.140 4.111 4.118 4.114 4.112
1086.
993 2036
445 646 5605
' 1095.610 992.997
2036.141 445.27T 46.280
5605.275
55.79 MM 51.01 bb 104.87 bb
' 22.73 MM 33.11 MM
289.16 bb
V/ /
278.00 278.00 278.00
4.116 4.112 4.114 4.117 .4.117
4.116 4.068 4.117 4.127 4.102 4.132 4.174 4*149 4.115
4.111 4.138 4.179 4.154 4.148 4.118
611 585 600 534 4623
. 1326573 1485431 569662 494912 582310 <459 245 331762 383053 302276 4714 115 6236 5869 6367
611.040
31.29 bb
585.165
29.95 MK
600.354
30.74 MM
cVu4 P5D& A\533.884
27.30 bb -
4822.879 248.76 bb _ -11
1326572.750 1485431.125
68495.76 76698.23
bb bb
J"' *
i
... ...
29413.54 bbs
494911.688 25553.92 bb
582309.750 30066.62 bb p - e t A b X S b
6459.212 333.25 bb
245.376
12.41- MM
331762.219 17129.89 bb
383053.431 19778.25 bb
302275.969 15607.40 bb
4714.130 243.15 I
-13
114.576
5. 65 MM
6236.417 321. 75 bb
5868.653 302. 76 bb ) E/tU tM
6366.802 328. 48 bb
* / io
g ) LAC os/os f a
m a d a lin a 04 1 0 9 9 ; '.q u a n ti t a ta d an d p r i n t a d b y n a a 5 /0 5 /9 9
000619
O O
o
a
K
mad*lina 041099:-.quantitat*d a nd printad b y a m 3/05/99
Tox-120 Hat U w
22S2SSSSSS2SSSSS.
11
III
I *E|U i !n ?
H%* ***I* a t-
! 3 5'3C 5
I iis*rs
s 2sa
s S58 s s
P 5 * " fc
I 111"
? 3
SSSS
I|& a|
ESESSisas:. S8SS8SSSX
2
8
sSSSSssaSESSsSSes
liiiis s ~ sssgl
iiilig g g BggS BSSSSS3SSS333SSSI
S 2 ! S 5 s 8 s . h MMS s 5 6
SSggSS2i2S:S2 ggSS3ggS3383ig&&3
. *H W A OH M* H MO D MH
8
i-J
j
<
rSP
Q u a n tify Compound B u m u ry R e p o rt Tox-120 R at L iv o r
Sample Lit: N:\rata2.PR0Vsaopla0B\04209Sa Loot modified: Thu Apr 22 09:02: li 1999
Method:
M:\rata2.PRO\MethD8\042099o
Laat modified: Tue Hey 04 0S:S2:4S 1999
Job Code:
Printed:
Rad May OS 11:10:S1 1999
Compound 4: IS
#Hama
Sampialiat Text
1 042099002
MeOH Blank
2 042099003
Toxl20, RBL04199-H20Blk-l, 4/19/99
3 042099004 4 042099005
OX120, RBL04199-LivBlk-l, 4/19/99 Toxl20, RBL04199-5ppb-l, 4/19/99
6S 042099006 042099007
TOX120, RBL04199-10ppb-l, 4/19/99 Toxl20, RBL04199-2Sppb-l# 4/19/99
7 042099008
Toxl20. RBL04199-50ppb-l, 4/19/99
8 042099009
Toxl20, m04199-100ppb-l. 4/19/99
9 042099010
Toxl20, KBL04199-2S0ppb-l, 4/19/99
u10 042099011 042099012
Toxl20, RBL04199-500ppb-l, 4/19/99 Toxl20, ABL04199-?S0ppb-l, 4/19/99
12 042099013
Taxi20 RSL04199-1000ppb-l, 4/19/99
13 042099014
MeOH Blank
14 042099015 15 042099016 16 042099017
Toxl20, RTL, IK. SI. 4/19/99 Toxl20. RTL. 2M, SI. 4/19/99 Toxl20. RTL, 3M, Gl. 4/19/99
17 042099018
TOX120. RTL, 4M, 61, 4/19/99
18 042099019
Toxl20. RTL, SM, Gl, 4/19/99
1 042099020
Toxl20, RBL04199-2S0ppb-CCl, 4/19/99
20 042099021
MeOH Blank
21 042099022
TOX120, RTL, M. Gl, 4/19/99
22 042099023
TOX120. RTL, 7H. Gl, 4/19/99
23 042099024
Toxl20, RTL. BH. Gl, 4/19/99
24 042099025
TOX120, RTL, 9M, Gl, 4/19/99
25 042099026 26 042099027
TOX120, RTL, 10M, Gl, 4/19/99 TOX120, RBL04199-250ppb-CC2, 4/19/99
27 042099028
MeOH Blank
28 042099029
Toxl20, RTL. 11M, 6 1 , 4/19/99
29 042099030
Toxl2Q, RTL, 13M, Gl, 4/19/99
30 042099031
TOX120, RTL, 14M, Gl, 4/19/99
31 042099032 ` TOX120, RTL, ISM, Gl, 4/19/99
32 042099033
Toxl20, RBL04199-2S0ppb-CC3, 4/19/99
33 042099034
MaOH Blank
34 042099035
TOX120, RTL, 17M, G2, 4/19/99
35 042099036
TOX120, RTL, ISM, G2, 4/19/99
36 042099037
TOX120, RTL, 19M, G2, 4/19/99
37 042099038
TOX120, RTL. 20M, G2, 4/19/99
38 042099039
TOX120, RTL, 21M, G2, 4/19/99
39 042099040
TOX120, RBL04199-2S0ppb-CC4, 4/19/99
40 042099041
MeOH Blank
41 042099042 '
TOX120, RTL, 22M, G2, 4/19/99
42 042099043
Toxl20, RTL, 23M. 62, 4/19/99
43 042099044
TOX120, RTL, 24M, G2, 4/19/99
44 042099045
TOX120, RTL, 25M, G2, 4/19/99
45 042099046
TOX120, RTL, 26H, G2. 4/19/99
46 042099047
TOX120, RBL04199-2S0ppfa-CCS, 4/19/99
47 042099048
MaOH Blank
48 042099049
Toxl20, RTL. 27M, G2, 4/19/99
49 042099050
TOX120, RTL, 29M, G2, 4/19/99
50 042099051
Toxl20, RTL, 30M, G2, 4/19/99
51 042099052
TOX120, RTL, 31M. G2, 4/19/99
52 042099053
TOX120, RBL04199-2S0ppb-CC6, 4/19/99
53 042099054 54 042099055
MeOH Blank TOX120, RTL, 33M..G3, 4/19/99
55 042099056 56 042099057. 57 042099058
TOXI20, RTL, 34M, G3, 4/19/99
Toxl20, RTL, 35M, G3, 4/19/99 Toxl20, RTL, 3SM, G3, 4/19/99
58 042099059
Toxl20, RTL, 37M, G3, 4/19/99
59 042099060
ToxI20, RBL04199~2S0ppb-CC7, 4/19/99
60 042099061
MeOH Blank
61 042099062
TOX120, RTL. 36H, G3, 4/19/99
62 042099063
TOX120, RTL, 39M, G3, 4/19/99
63 042099064
TOX120, RTL, 4QM, G3, 4/19/99
64 042099065
TOX120, RTL, 41M, G3, 4/19/99
65 042099066
TOX12Q,* RTL, 42M, G3, 4/19/99 '
66 042099067
TOX120, RBL04199-230ppb-CCS, 4/19/99
67 042099068
MeOH Blank
68 042099069 69 042099070 70 042099071 71 042099072 72 042099073
TOX120, RTL. 43H, G3, 4/19/99 TOX120, RTL, 4SM, G3, 4/19/99 TOX120, RTL, 44M, G3, 4/19/99
Toxl20, RTL, 47M, 63, 4/19/99 ' TOX120, RBL04199-2SOppb-CC9, 4/19/99
73 042099074 74 042099075
MeOH Blank TOX120, RTL, SSM, GS, 4/19/99
75 042099076 76 042099077
TOX120, RTL, (SK, GS, 4/19/99 TOX120, RTL, 73M, GS, 4/19/99
77 042099078
TOX120, RTL, 74M, G5, 4/19/99
78 042099079
TOX120, RTL, 7SH, GS, 4/19/99
79 042099080
TOX120, RBL04199-2S0ppb-CC4, 4/19/99
80 042099081
MeOH Blank
81 042099082
TOX120, RTL, 77M, GS, 4/19/99
82 042099083
Toxl2Q, RTL, 78M, GS, 4/19/99
83 042099084
Toxl20, RTL, 79M, GS, 4/19/99
84 042099085
TOX12Q, RBL04199-250ppb-CCS, 4/19/99
65 042099086 * 86 042099087
MeOH Blank TOX120, RTL, 3H, Gl-2S0ppb-M5, 4/19/99
87 042099098
TOX120, RTL, 3M, Gl-2S0ppb-MS0, 4/19/99
86 042099099
TOX120, RTL, 6M, Gl-2S0ppb-MS, 4/19/99
TVpe
S td C otta
Standard
Standard Standard
Standard Standard Standard Standard Standard , Standard
1.00
1 a00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
QC 1.00
OC .
QC
1.00 1.00
QC 1.00
QC 1.00 QC 1.00
QC 1.00
QC 1.00 QC 1.00
QC 1.00 .
QC 1.00
xr
4.67 4.477 4.477 4.475 4.472 4.474 4.473 4.481 4.4-78 4.475 4.47
4.477 4.473 4.479 4.473 4.480 4.477
4.479 4.476 4.478 4.478 4.476 4.475
4.476 4.479 4.479 4.476 4.479
4.474 4.476 4.475 4.465 4.434 4.471
4.473 4.474 4.438 4.434 4.434 4.473
4.478 4.434 4.473 4.429 4.478
4.475 4.474 4.430 4.445 4.448 4.445
4.467 4.456 4.426 4.437 4.475 4.475
4.434 4.474 4.475 4.435 4.478
4.478 4.475 4.434 4.442 4.461 4.449
.4.465 4.473 4.428 4.453
4.472 4.466 4.480
A to m
11168 14262 17108 14220 14163 15047 14165 12687 13127 12184 14980
13354 15480 16743 16108 16428 12965
12592 12908 14767 13113 . 16767 15329
. 14482 11815 13252 12072 12820
7188 6839 6975 7305 7576 15008
7420 8272 8265 9236 9268 14716
8715 12268 10776 11197 14400
16537 8276 7717 9574 7747
15240
7589 9947s 7538 8461 12183 .14952
8922 9854 13112 9645 15910
10254 11430 14854 14617 14512 15752
26825 16234 16282 15674
15718 17154 15585
tOvX l t p O R M C o tta flag *
11168.010 14262.124 17107.816 14220.119
14162.521 15047.055
14164.800 12686.997
13127.305 12183.721 14980.457
0.72 bb
0.92 bb
1.10 bb 0.92 bb 0.91 bb 0.97 bb
0.91 bb 0.82 bb
0.85 bb 0.79 bb 0.97 M M
-28 -8
10 -8
-9 -3 -9 -19 -15 -21 -3
13353.618 15479.964 16743.496 16107.631 16427.918
12964.758
0.96 bb 1.00 bb 1.09 bb 1.04 bb 1.06 bb
0.84 bb
-14 0 8
4 6 -16
12591.617 12908.252
14767.411 13113.133 16766.613 15328.990
0.81 bb
0.83 bb 0.95 bb . 0.85 bb 1.08 bb 0.99 bb
-19 -17
-5
-158
-1
14482.320 11815.149
13252.295 12071.706 12819.677
0.93 bb
0.76 bb 0.86 bb 0.78 bb 0.83 bb
-7 -24 -14 -22 -17
7188.418 6839.444 6975.442 7304.985 7576.105 15007.997
0.46 bb 0.44 bb 0.45 bb 0.47 bb 0.49 bb 0.97 bb
-54 -56
-55 -53
-51 -3
7419.602 8272.232 8264.774
9235.608 268.021 14716.280
0.48 bb 0.53 bb 0.53 bb 0.60 MM 0.60 bb 0.95 bb
-52 -47
-47 -40 -40
-5
8714.930 12268.174 10776.486 11196.844 14399.545
0.56 bb
0.79 bb 0.70 bb,
0.72 bb 0.93 bb
,
-44 -21 -30 -28
-7
16537.041 8276.272 7716.823 9574.318
7747.233 15240.495
1.07 bb 0.53 bb
0.50 bb 0.62 bb 0.50 bb
0.98 bb
7 -47
-50 -38 -50
-2
7588.534
9947.499 7537.861 480.613 12182.729 14951.941
0.4 bb 0.64 bb 0.49 bb 0.55 bb 0.79 bb 0.97 bb
-51 -36
-51
-45 -21
-3
8921.938 9854.359 13111.587 .
9645.204 15909.816
0.58 MM
0.64 bb
0.85 bb 0.62 bb 1.03 bb
-42 -36
-15 -38
3
10253.570 11430.374
14853.702 14617.353 14511.814 15752.205
0.66 bb
0.74 bb 0.96 MM 0.94 bb
0.94 bb 1.02 bb
-34 -26
-4 -6
-6
2
26824.648
16234.444 16281.634 15674.262
1.73 bb 1.05 MM 1.05 MM
1.01 bb
73 5 S
1
15717.900
17154.322 15585.048
1.01 bb 1.11 bb 1.01 bb
1
11 1
Pape 7
M ad elin e 0 4 1 0 9 9 :q u a n tita te d and p r in te d b y m aa 5 /0 S /9 9
000621
Q u a n tif y Com pound Summary R a p o rt Tox-120 S a t L iv a r
Sample Liat: H:\rata2.PSO\SaapleDB\Q42099e
Laat modified: Thu Apr 22 09:02:11 1999
Mathod:
N :\rata2. VS0\MethD8\O42O99o
Laat modified: Tua Hay 04 09:52:49 1999
Job Coda:
Printed:
Wad Hay 05 11:10:51 1999
Compound 4: 15
Mama 99 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 95 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105
Saapl*liat Taxt
Typ*
Std'Cono
TOX120, RTL, CM, Gl-250ppb-MSD, 4/19/99
TOX120, RTL, 13M, Gl-2S0ppb-MS, 4/19/99
TOX120, RTL, 13H, 61-2S0ppb-M5D, 4/19/99
OX120, RBL04199-2iOppb-CC, 4/19/99
QC
1.00
HaOH Blank
TOX120, RBL04199-H2OBlk-l, 4/19/99
TOX120. RBL04199-LivBlk-l, 4/19/99 TOX120, RBL04199-- 5ppb~l, 4/19/99
TOX120, RBL04199-10ppb-l, 4/19/99 TOX120, RBL04199-25ppb-l, 4/19/99
TOX120, RBL04199-S0ppb-1, 4/19/99 TOX120, RBL04199-100ppb-l, 4/19/99 TOX120, RBL04199-2S0ppb-l, 4/19/99
TOX120, RBL04199-S00ppb>l, 4/19/99 TOX120, RBL04199-7SOppb-l, 4/19/99 Toxl20, RBL04199-1000ppb-l,-4/19/99
Standard Standard Standard Standard . Standard
Standard Standard Standard Standard
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
M 4.477 4.478 4.435 4.470
4.478 4.478 4.473 4.435 4.433 4.434 4.433 4.465 4.434 4.434 4.435
Pago 8
Araa 15039 14583 15562 16369
13605 17110 20728 17142 16514 17079 17088 15845 15402 15262 16108
Response 15038.934 14562.552 15562.266 16367.936
13604.745 17109.813 20728.434 17141.998 16513.951 17078.793 17087.787 15644.741 15402.126 15261.598 16108.356
Cone. Flaoa %Dav
0.97 bb
-3
0.94 bb
-6
1.00 bb
0
1.06 bb
6
0.68 bb
1.10 bb 1.34 bb 1.11 bb 1.07 bb 1.10 bb 1.10 bb 1.02 bb
0.99 bb 0.99 MM 1.04 bb
-12 10 34 11 7
10 10
2 -1 -1 4
.!>t
m ad elin e 0 4 1 0 9 9 ::q u a n tita ta d and p r in ta d b y aa a 5 /0 5 /9 9
000622
Q u a n tify C a lib ra tio n fiap o rt T ox-120 H at L iv a r
Calibration s W:\rata2.PH0\CurvaDB\042099o Last tnodifiad: W o d M ay 05 09:00:19 1999
Printad:
Wad May 05 11:11:10 1999
^Compound 1 name: POAA Coefficient o f Detennination: 0.989036
1
m ad alin a 0 4 1 0 9 9 :tq u a n tita ta d and p r in ta d b y n aa 5 /0 5 /9 9
000623
Q u an tify C a lib ra tio n R aport Tox-120 R a t L iv a r
Calibration: M:\rata2.FRO\Curva0B\042099a
Laat nodifiad: Had May OS 09:00:19 1999
Frintad:
Rad May OS 11:11:10 1999
Vago 2
m ad a lin a 041099: :q u a n ti ta ta d and p r in ta d b y m an 5 /0 5 /9 9
000624
Q u in tiy C a lib ra tlo n R aport Tox-120 R at L iv a r
Calibration: M:\rata2.VRO\CurvaDB\042099e
List nodifiad: Rad May 03 09:00:19 1999
Printad:
Rad Kay OS 11:11:10 1999
Vaga 3
n id a lin a 041099: : q u a n ti ta t a d an d p r in ta d b y M a S/O S/99
000625
Quantify Calibration Kaport Tea-120 tat Llvar
Calibration: M:\rats2.mo\CurvaDB\042099a
Last nodifiad: Wad May OS 09:00:19 1999
Prlntad:
Wad May OS 11:11:10 1999
'
Pag 4
a a d a lin a 041099: iq u a n tita ta d and. p r in ta d b y n aa S /0 S /9 9
000626
H.thod Report Method t i l t : Last modiil.d: Compound: Printed:
N:\raCs2.PRO\HethOB\042099c.mdb Tu Hey 04 0S:S2:4S 1999C
1: POAA
Had Hey OS 11:10:2 1999
Compound N am: Internal Ref.: Qutnify Traa:
Conc. of Standards:
POAA (Nona) TIC Cone. A
Usar RF Vala:
Usar Psak Factor: Integrate Window Factor:
1.000000, Dlsablad 1.000000 2.000
Peak Location
Ratantion Tima (mine):
Relative Ratantion Tina: Tina Window (nina):
4.400, Enabled 0.000, Disabled
0.834
Faak Salactlon:
Nearest
Rasoonaa
] Raaponsa Typa: Rasponsa:
Calibration Curves
External Areas
Polynomial Typa:
1 Point of Origin: Fit Weighting: Axis Transformation: .
Linear
Exclude 1/X
Nona
Concentration
] Units: Noise
Peak-to-'peak amplitude
434
Smooth
Smoothing:
Window sisa (scans): Numbar of smooths: Smoothing method:
Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing:
Raisa basaliaa maximum:
20.00
30.00 5.00
Peak Senaration
Draw vertical threshold:
Detect shoulders:
Shoulder threshold:
:
10.00
Disabled 40.00
J Response Threshold Relativ height:
Absolute height: Relative area: Absolute area:
1.50, Disabled 0.00, Disabled ' 2.00, Disabled 0.00, Disabled
3
]
I
l'*0. 1
I
LAC/ oslof h ?
t* *
t
`f i f
000627 r * !
K.tfiod Report
Method file: Last modified:
Compound:
Printed:
N : \ r t j 2 .P R O \ M * t h O B \ 0 4 2 0 9 9 c . a d b Tu. May 04 04:52:4* 19990
2: Pros
Had Hay 05 11:10:26 1999
Compound Name:
Internal Ref.: Quantity Trace: Cone*, of Standards
pros (Hone]
TIC Cone. A
User ftp Value:
User Peak Factor: Integrate Window Factor;
1.000000, Disabled 1.000000
2.000
Peek Location
Retention Time Ulna): Relative Retention Tine: Time Window (mins):
4.790, Enabled 0.000, Disabled 0.034
Peak Selection:
Nearest
Response
Response Type: Response:
Estensi Areas
Calibration Curves
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
Linear Exclude 1/X
None
Concentration
Units:
Noise
Paak-to-peak amplitude:
434
Smooth
Smoothing; Window size (scans)
Number of smooths: Smoothing method:
Disabled
3 2
Baselines
Join valley threshold: Reduce peak tailing:
Raise baseline maximum:
20.00 50.00
a. 5.00
Peak Seoaration
Draw vertical threshold:
Detect shoulderj;
Shoulder threshold:
.
10.00
Disabled 40.00
Reseonse Threshold
Relative height: Absolute height: Relative area:
Absolute area:
1.50, Olaablad
0.00, Disabled * 2.00, Diaablad
0.00, Disabled
Rage 2
M fljtlihi, Wl(M lAC. n ila tk _ _ _ _ _ _ _
A*4
000628
Method Report
Method file: Lest modified:
Compound:
Printed:
N:\rats2.PRO\MethDB\042099c.mdb Tue May 04 00:52:48 19990
3: PFHS
Med May 05 11:10:26 1999
Compound Name: Internal Ref.: Quantify Trace: Cone', of Standards:
User RF Value: User Peak Factor: Integrate Window Factor:
Peak Location
Retention Time (mins): Relative Retention Time: Time Window (mins]:
Peak Selection:
Response
Response Type: Response:
Calibration Curves
Polynomial Type: Point o Origin: Fit Weighting: Axis Transformation:
Concentration
Units:
Noise
Peak-to-peak amplitude:
Smooth
Smoothing: Window size (scans): Number of smooths: Smooching method:
Basellnes
Join valley threshold; Reduce peak tailing: Raise baseline maximum:
Peak Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold:
Response Threshold
Relative height: Absolute height: Relative aree: Absolute area)
PFHS (None) TIC Cone. A 1.000000, Disabled 1.000000 2.000
4.120, Enabled 0.000, Disabled 0.834 Nearest
External Areas
Linear Exclude 1/X None
434
Disabled 3 2
20.00 50.00 5.00
10.00 Disabled 40.00
1.50, Disabled 0.00, Disabled 2.00, Disabled 0.00, Disabled
H & cI lL , OHIdW LAC/ o r / c ^ M
3
.X
000629
Mechad Report
Method file: Last Modified:
Compound:
Printed:
N:\r.t32.PR0\M.thDB\042099e.mdb Tue May 04 08:52:49 1999Q
4: IS
Ned M ay OS 11:10:26 1999
Compound Name: Internal Ref.:
Quantify Trace: ConC of Standard:
, IS
(None) TIC Fixed
User RF Value: User Peak Factor: Integrate Window Factor:
1.000000* Disabled 1.000000 2.000
Peak Location
Retention Time (mins): Relative Retention Time:
Time Window (mins):
4.480, Enabled
0.000, Disabled 0.834
Peak Selection:
N.r.it
Resoonse
Response Type: Response:
External Areas
Calibration Curves
Polynomial Type:
Point of Origin: Tit Weighting: Axis Transformation:
Linear Exclude 1/X
None
Concentration
Unita:
Noise
Peak-to-peak amplitude:
434
Smooth
Smoothing:
Window sire (scans): Number of smooths: Smoothing method:
Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing:
Raise baseline maximum:
20.00 50.00 5.00
Peak Separation
Draw vertical threshold:
Detect shoulders:
Shoulder threshold:
t
10.00 Disabled 40.00
Response Threshold
Relative height: Absolute height:
Relative area: Absolute area:
1.S0, Disabled 0.00, Disabled
4 2.00, Disabled 0.00, Disabled
M a A tU
c h ip
Pag* 4
000630
AM ELIA062498: FACT Equipm ent Log 99016
Q uattro H , #6448E
PAGE
DATE: 04/20/99
ANALYST: mee
STUDY NUMBER Tox-120 MATRIX: R at Liver DESCRIPTION: ip
FACT M ethod: ETS-8-5.0________
Injection volume,10 uL
Cycle tim e: 9.0 m in. Electrode: Z-Spray
M obile P hase:
(A12.0 mM NH4GH3CQ2
(B)M eO H
TN-A- or stndrd num ber (A ) 99012-5-22_________
(B) TN-A-2414
Prepared (date/by):
(A) 4/16/99 / ok_________
(B) 4/9/99 hoj
E x p ires:
(A) 10/99_______________
(B) 10/99
Store at room temp? |Y| o r N
O ther:___________________
G u ard colum n: N one ______________
P art num ber na_________
Lot number: na______________________
A nalytical colum n: keystone betasil
___________________________________
Size: 2 x 50mm_________________ Type: c l8____________ Sphere size: 5 um
Part number: 055-701-2
L ot num ber P7L04 Serial num ber 0481172z
A nalytical param eters:
t
D rying gas (L/hr): 300
ESI nebulizing gas (L/hr) 15
LC Flow rate: 300 ulVmin. Back pressure: 59 bar
Sheath gas (L/hr)_0___
H PLC solvent g rad ien t: long2
Time (m in.) 1) 0.00________ 2) 4.5__________ . 3 6.5_________ 5) 7.0_________ 6) 9.0__________
%B 40 95 95 40 stop
000631
Tuning Method Report
Method:
C:\MASSLYNX\RATS2.PRO\ACQUDB\PFOS
Printed:
Tue Apr 20 17:06:38 1999
' Page 1
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 15
5 0.2 150 250
2.99 -14 -6
152 258
Pressures
Analyser Vacuum Cas Cell
Rdbk
1.6e-4 4.7e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
Set Rdbk
15.0 15.0 2.0 s o.o- 57 650
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rd
15.0 15.0
30 2.0 0.0
40 0 650
32
39 30
000632
FACT: DATA REVIEW SUMMARY FORM
Date:
4 /^ /5 2
Analyst:
H
FACT Studv Number W ' M
Matrix
ILf
Tuna Pointa
M f 4
Dilutions?
YJLtdJ-
Analyte 1: AcccotableT,mftarRange:
LOQ for Aia data set _______ ^ Reevaluated for differentrange?
M
Analyte 4:
Acceptable Linear Range:
/
LOQ for this data set
/
Re-evaluated for differentrange? /
/ ____________________ _____
for different range?
Analvte2:
./
Acceptable Linear Range:
LOQ for this data set
.f
Re-evaluated for different range? - J . ____________
Analyte 3:.
Acceptable Linear Range:
LOQ for this data set:
Re-evaluated far different
Analyte 6: Acceptable linear Range: LOQ for diis data set Re-evaluated for differentrange?
Analyte 7: Acceptable Linear Range: LOQ for this data set Re-evaluated for different range?
h ilL j
ly v d r C fiv J e l b * k u /b * ~ '
000633
Q u a n tif y Com pound flutto*r y l u p o r t
Saaplo Lit: C:\MA8SLYKX\rata2. FR0\SaoplaDB\042099d
Last aodifiad: Thu Apr 22 07:25:34 1999
Mathod:
C:\HA38LYHX\rmta2. PRO\MothDB\042099d
Last oodifiad: Thu Apr 22 07:23:52 1999
Job Coda:
Printod:
Thu Apr 22 07:31:55 1999
Compound 1$ "
I iiaaa 1 04209910
2 042099107 3 042099100 4 042099109 5 042099110 0 042099111 7 042099112 8 042099113 9 042099114 10 042099115 11 042099116 12 042099117 13 042099110 14 042099119 15 042099120 16 042099121 17 042099122 U 042099123 19 042099124 20 042099125 21 042099126 22 042099127 23 042099128 24 042099129 25 042099130 26 042099131 27 042099132 28 042099133 29 042099134 30 042099135 31 042099136 32 042099137 33 042099138 34 042099139 35 042099140 36 042099141 37 042099142 38 042099143 39 042099144 40 042099145 41 042099146 42 042099147 43 042099148 44 042099149
Saxploliat Taxt MtOH BlftJe 10x120, RBL04199-H20Blk-l, 4/19/99
Toxl20, RBL04199-LivBlk-l, 4/19/99
TOX120, RBX04199-5ppb-l, 4/19/99 TOX120, RBL04199-10ppb-l, 4/19/99 TOX120, ABL04199-2Sppb-l, 4/19/99 TOX120, RBt04199-50ppb-l, 4/19/99
Toxl20, RBL04199-100ppb-l, 4/19/99 Toxl20, RB104199-250ppb-l, 4/19/99 TOX120, RBL04199-S00ppb-1, 4/19/99
TOX120, RBL04199-750ppb-l, 4/19/99
TOX12Q, RBL04199-1000ppb-l, 4/19/99 KaOH Blank
TOX120, ATI, 49H, 64, 4/19/99 TOX120, RTL, 50M, 64, 4/19/99 Toxl20, RTL, 51M, 64, 4/19/99
TOX120, RT1, S2M, 64, 4/19/99. TOX120, RTL, 53M, 64, 4/19/99
TOX120, RBL04199-2$0ppb-CCl, 4/19/99
MaOK Blank Toxl20, RTL, 54M, 64, 4/19/99
Toxl20, RTL, 55H, 64, 4/19/99
TOX120, RTL, 56M, 64, 4/19/99 TOX120, RTL, 57M, 64, 4/19/99
oxl20, RTL, S8H, G4, 4/19/99 TOX120, RBL04199-250ppb-CC2, 4/19/99
HaOH Blank TOX120, RTL, S9M, G4, 4/19/99 Toxl20, RTL, 61M, 64, 4/19/99 Toxl20, RTL, 62M, 64, 4/19/99 TOX120, RTt, 63M, G4, 4/19/99 TOX120, RBL04199-250ppb-CC3, 4/19/99
KeOK Blank Toxl20, RBU)4l99-H20Blk-l, 4/19/99
TOX120, RBU>4l99-LivBlk-l, 4/19/99
TOX120, RBL04l99-5ppb-l, 4/19/99 TOX120, RBL04199*1Oppb-1, 4/19/99
TOX120, RB104199-25ppb-l, 4/19/99 TOX120, RBl04l99-50ppb-l, 4/19/99
Toxl20, RB104199-I00ppb-1, 4/19/99 TOX120, RB104199-250ppb-l, 4/19/99 Toxl20, RBI*04199-S00ppb-1, 4/19/99 Toxl20, RBI*04199-7S0ppb-l, 4/19/99 TOX120, RBL04199-1000ppb-l, 4/19/99
Pag 1
Typa
Standard Standard Standard Standard Standard Standard Standard Standard Standard
QC
OC
QC
Standard Standard Standard Standard Standard Standard Standard Standard Standard
fltd Ceno
5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00
250.00
250.00
250.00
5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00
RT 4.631 4.574 4.770 4.766 4.770 4.768 4.746 4.743 4.746 4.743 4.743 4.745 4.436 4.728 4.746 4.754 4.745 4.747
4.731 4.502
4.746 4.724 4.747
4.791 4.764 4.742 . 4.502 4.766 4.756 , 4.770 4.762 4.747 4.418 4.528 4.768 4.766 4.764 4.763 4.765 4.766 4.763 4.768 4.750 4.755
A m Cono. Plaga %Dav
12 0.00 b b
40 0.95 bb
36 0.00 bb
57 5.24 bb
5
89 13.82 MM
39
155 31.66 MM
27
247 56.22 bb
12
485 120.05 bb
20 *
1112 287.94 MM
15
2185 575.32 bb
IS
2976 787.41 bb
5
3297 873.36 bbX
-13
87 13.52 bb
12991 3470.15 bb
6082 1619.43 bb
5703 1517.85 bb
I
7024 1871.64 bb
2063 543.96 bb
1057 273.37 bb
9
100 16.96 MM 6163 1641.02 bb
rv
9346 2627*60 bb
11879 3172.37 bb
651 164.53 bb
150 30.42 bb
1017 262.59 bb *
5
124 23.19 bb
772 196.94 bb
155 31.70 bb
189 40.35 bb
60 6.26 bb
977 251.92 MM
1
107 18.64 bb
26 0.00 bb
33 0.00 bb
51 3.82 bb -24
S3 4.34 bb -57
111 19.80 bb
-21
225 50.35 MM
1
397 96.32 MM
-4
864 221.52 bb
-11
1745 457.44 bb
-9
2459 648.75 bb
-13
2343 7S1.70 MMX
-25
p.
Amalia 062493: Raviavad and quanti tatad by kjh 4/22/99
000634
Q u an tity C a lib ra tio n a n p o rt
Calibrations C:\MASSUmx\rata2.91IO\CurvaDB\042099d
Laat noditlad: Thu Apr 22 07:30:53 1*9
Printed:
Thu Apr 22 07:31:55 19*9
Pago 1
Anal la 052495: Aavlawad and quantitated by k)h 4/22/9
000635
Masslynx - Sample List
Sample Ust: C:\MASSLYNX\rats2.PRO\SampleDB\042099c.SPL Printed: Tue Apr 20 16:51:50 1999
F ile Nam e
F ile T ext
1 042099002
MeOH Blank
2 042099003
Tox120, R B L04199-H 2O B Ik-t, 4/19/99
3 042099004
Tox120, RBL04199-LivBlk-1,4 /1 9 /9 9
4 042099005
Tox120, R B L04199-5ppb-1,4/19/99
5 042099006
Tox120, R B L04199-10ppb-1,4/19/99
6 042099007
Tox120, RBL04199-25ppb-1,4 /1 9 /9 9
7 042099008
Tox120, R B L04199-50ppb-1.4/1 9 /9 9
8 042099009
Tox120, RBL04199-100ppb*1,4 /1 9 /9 9
9 042099010
Tox120, RBL04199-250ppb-1,4 /1 9 /9 9
10 042099011
TOX120, R BL04199-500ppb-1,4 /1 9 /9 9
11 042099012
Tox120, RBL04199-750ppb-1,4 /1 9 /9 9
12 042099013
Tox120, RBL04199-1000ppb-1,4 /1 9 /9 9
13 042099014
MeOH Blank
14 042099015
Tox120, RTL, 1M , G 1 ,4/1 9 /9 9
15 042099016
Tox120, RTL. 2M , G 1 ,4/1 9 /9 9
16 042099017
Tox120, RTL. 3M . G 1 .4/1 9 /9 9
17 042099018
Tox120, RTL. 4M . G 1 .4/19/99
18 042099019
Tox120, RTL. 5M , G 1 ,4/1 9 /9 9
19 042099020
Tox120, R B L04199-250ppb-C C 1,4/1 9 /9 9
20 042099021
MeOH Blank
21 042099022
Tox120, RTL, 6M . G 1 ,4/19/99
22 042099023
Tox120, RTL, 7M , G 1 .4/19/99
23 042099024
Tox120, RTL, 8M , G 1 ,4/19/99
24 042099025
Tox120, RTL, 9M , G 1 ,4/19/99
25 042099026
Tox120, RTL. 10M , G 1 ,4/19/99
26 042099027
Tox120, R B L04199-250ppb-C C 2,4/19/99
27 042099028
MeOH Blank
28 042099029
Tox120, RTL, 11M , G 1 ,4/19/99
29 042099030
Tox120, RTL, 13M , G 1 ,4/19/99
30 042099031
Tox120, RTL, 14M , G 1,4 /1 9 /9 9
31 042099032
Tox120, RTL, 15M , G 1,4 /1 9 /9 9
32 042099033
Tox120, R B L04199-250ppb-C C 3,4/19/99
33 042099034
MeOH Blank
34 042099035
Tox120, RTL, 17M , G 2 ,4/19/99
35 042099036
Tox120, RTL. 18M , G 2 ,4/19/99
36 042099037
Tox120, RTL, 19M , G 2 ,4/19/99
37 042099038
Tox120, RTL, 20M , G 2 ,4/19/99
38 042099039
Tox120, RTL, 21M , G 2 ,4/19/99
39 042099040
Tox120, R B L04199-250ppb-C C 4,4/19/99
40 042099041
M eO H Blank
41 042099042
Tox120, RTL. 22M , G 2 ,4/19/99
42 042099043
Tox120, RTL, 23M , G 2 ,4/19/99
43 042099044
TOX120, RTL, 24M , G 2 .4/19/99
44 042099045
T o x t2 0 , RTL, 25M , G 2 .4/19/99
45 042099046 Tox120, RTL, 26M , G 2 ,4/19/99
46 042099047
Tox120, R B L04199-250ppb-C C 5,4/19/99
47 042099048
M eO H Blank
48 042099049
Tox120, RTL, 27M , G 2 ,4/19/99
49 042099050
Tox120, RTL, 29M , G 2 ,4/19/99
50 042099051
Tox120, RTL, 30M , G 2 ,4/19/99
51 042099052
Tox120, RTL, 31M , G 2 ,4/1 9 /9 9
52 042099053
Tox120, R B L04199-250ppb-C C 6,4/19/99
53 042099054
MeOH Blank
54 042099055
TOX120, RTL, 33M , G 3 ,4/1 9 /9 9
55 042099056
Tox120, RTL, 34M , G 3 ,4/19/99
56 042099057
Tox120, RTL, 35M , G 3 ,4/1 9 /9 9
57 042099058
Tox120, RTL, 36M , G 3 ,4/19/99
58 042099059
Tox120, RTL, 37M , G 3 ,4/19/99
59 042099060
Tox120, R B L04199-250ppb-C C 7,4/19/99
60 042099061
MeOH Blank
y /^ y y 7 Page 1
MS File
In let File
9MIN-COMBI7IS-POAA 9mln
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9mln
9M IN-COMBI7IS-POAA 9min
9MIN-COMBI71S-POAA 9min
9M IN-COMBI7IS-POAA 9mln
9MIN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9mln
9MIN-COMBI71S-POAA 9min
9M IN-COMBI7IS-POAA 9mln
9M IN-COMBI7IS-POAA 9mln
9M1N-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min
. 9MIN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
. 9MIN-COMBI7IS-POAA 9mln
9MIN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9mln
9MIN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9niln
9M IN-COMBI7IS-POAA 9min
9MIN-COMB17IS-POAA 9min
9M IN-COMBI7IS-POAA 9mln
9MIN-COMB17IS-POAA 9min
9MIN-COMBI7IS-POAA 9mln
9MIN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA - 9min
9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9mln
9M IN-CO M BI7IS-PO A A . 9mln
9M IN-COMBI7IS-POAA . 9min
9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9mln
9M IN-COMBI7IS-POAA 9mln 9M IN-COMBI7IS-POAA 9mln
9M IN-COMBI7IS-POAA 9min
9M IN-COMB17IS-POAA 9 M IN -C O M B 17IS -P O A A 9M IN-COMBI7IS-POAA
9min 9mln 9mln
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9mln 9M IN-COMBI7IS-POAA 9mln
9M1N-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9M IN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9min
9M IN-COM B17IS-POAA 9min
9M IN-COMBI7IS-POAA 9min 9M IN-COMBI7IS-POAA 9mln
B o ttle
91 1 2 3 4 5 6 7 8 9 10 11 * 91 12 13 14 15 16 81 92 17 18 19 20 21 82 92 22 23 24 25 83 92 26 27 28 29 30 84 92 31 32 33 34 35 85 92 36 37 38 39 86 93 40 41 42 43 44 87 93
000636
Masslynx - Sample List
Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\042099c.SPL Printed: Tue Apr 2016:51:50 1999
F ile Nam e
61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 66 042099067 67 042099068 68 042099069 69 042099070 70 042099071 71 042099072 72 042099073 73 042099074 74 042099075 75 042099076 76 042099077 77 042099078 78 042099079 79 042099080 80 042099081 81 042Q99082 82 042099083 83 042099084 84 042099085 85 042099086 86 042099087 87 042099088 88 042099089 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 96 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105
F ile T ext
Tox120, RTL, 38M , G 3 ,4/19/99 Tox120, RTL, 39M , G 3 .4/19/99 TOX120, RTL, 40M , G 3 ,4/19/99 Tox120, RTL, 4 1 M , G 3 ,4/19/99 Tox120, RTL, 42M , G 3 ,4/19/99 Tox120, R B L04199-250ppb-C C 8,4/19/99 MeOH Blank TOX120, RTL, 43M , G 3 ,4/19/99 TOX120, RTL, 45M , G 3 ,4/19/99 Tox120, RTL, 46M , G 3 ,4/19/99 Tox120, RTL, 47M , G 3 ,4/19/99 Tox120, R B L04199-250ppb-C C 9,4/19/99 MeOH Blank TOX120, RTL, 65M , G 5 ,4/19/99 Tox120, RTL, 66M , G 5 ,4/19/99 Tox120, RTL, 73M , G 5 ,4/19/99 TOX120, RTL, 74M , G 5 ,4/19/99 TOX120, RTL, 75M , G 5 ,4/19/99 Tox120, R B L04199-250ppb-C C 4,4/19/99 MeOH Blank Tox120, RTL, 77M , G 5 ,4/19/99 Tox120, RTL, 78M , G 5 ,4/19/99 Tox120, RTL, 79M , G 5 ,4/19/99 TOX120, RBL04199-250ppb-CC5, 4/19/99 M eO H Blank Tox120, RTL, 3M , G1-250ppb-M S, 4/19/99 TOX120, RTL, 3M . G1-250ppb-M SD, 4/19/99 TOX120, RTL, 6M , G1-250ppt>-MS, 4/19/99 Tox120, RTL, 6M , G 1-250ppb-M SD, 4/19/99 Tox120, RTL, 13M , G1-250ppb-M S, 4/19/99 Tox120, RTL, 13M , G1-250ppb-M SD, 4/19/99 Tox120, R BL04199-250ppb-C C 6,4/19/99 M eO H Blank Tox120, R B L04199-H 2O B Ik-1,4/19/99 Tox120, RBL04199-LivBlk-1,4 /1 9 /9 9 TOX120, RBL04199-5ppb-1,4 /1 9 /9 9 TOX120, RBL04199-1 Oppb-1,4 /1 9 /9 9 TOX120, R B L04199-25ppb-1,4/19/99 Tox120, RBL04199-50ppb-1,4 /1 9 /9 9 Tox120, RBL04199-100ppb-1,4/19/99 TOX120, RBL04199-250ppb-1,4 /1 9 /9 9 Tox120, R BL04199-500ppb-1,4/19/99 TOX120, RBL04199-750ppb-1,4 /1 9 /9 9 TOX120, RBL04199-1000ppb-1,4 /1 9 /9 9
Page 2
MS File
9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M N-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M INrCOM BI7IS-POAA . 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M 1N -C O M B I7IS -P O A A 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN -C O M B I71S -P O A A 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M 1N -C O M B I7IS -P O A A 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA 9M IN-CO M BI7IS-POAA
In let F ile
9min 9min 9min 9min 9mln 9min 9min 9min 9min 9mln 9min 9min 9min 9min 9mln 9mln 9min 9min 9mJn 9m in. 9min 9min 9min 9min 9mln 9min 9min 9min 9mln 9mln 9min 9mln 9min . 9min 9min 9min 9min 9min 9mln 9min 9min 9mln 9mln 9mln
B ottle
45 46 47 48 49 88 93 50 51 52 53 89 93 68 69 70 71 72 84 94 73 74 75 85 95 76 77 78 79 80 90 86 94 1. 2 3 4 5 6 7 8 9 10 11
000637
Masslynx Sample List
Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\042099d.SPL Printed: Tue Apr 2016:51:40 1999
-
File Name
1 042099106 2 042099107 3 042099108 4 042099109 5 042099110 6 042099111 7 042099112 8 042099113 9 042099114 10 042099115 11 042099116 12 042099117 13 042099118 14 042099119 15 042099120 16 042099121 17 042099122 18 042099123 19 042099124 20 042099125 21 042099126 22 042099127 23 042099128 24 042099129 25 042099130 26 042099131 27 042099132 28 042099133 29 042099134 30 042099135 31 042099136 32 042099137 33 042099138 34 042099139 35 042099140 36 042099141 37 042099142 38 042099143 39 042099144 40 042099145 41 042099146 42 042099147 43 042099148 44 042099149
File Text
MeOH Blank Tox120, RBL04199-H20Blk-1,4/19/99 Tox120, RBL04199-UvBlk-1,4/19/99 Tox120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-10ppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4/19/99 Tox120, RBL04199-50ppb-1,4/19/99 Tox120, RBL04199-100ppb-1,4/19/99 Tox120, RBL04199-250ppb-1, 4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99 MeOH Blank Tox120, RTL, 49M, G 4 ,4/19/99 Tox120, RTL, 50M, G 4 ,4/19/99 Tox120, RTL, 51M, G 4 ,4/19/99 Tox120, RTL, 52M, G 4 ,4/19/99 Tox120, RTL, 53M, G 4 ,4/19/99 Tox120, RBL04199-250ppb-CC1,4/19/99
MeOH Blank Tox120, RTL, 54M, G 4 ,4/19/99. TOX120, RTL, 55M, G 4 ,4/19/99 Tox120, RTL, 56M, G 4 ,4/19/99 TOX120, RTL, 57M, G 4 ,4/19/99 Tox120, RTL, 58M, G 4 ,4/19/99 Tox120, RBL04199-250ppb-CC2,4/19/99 MeOH Blank Tox120, RTL, 59M, G 4 ,4/19/99 Tox120, RTL, 61 M, G 4 ,4/19/99 Tox120, RTL, 62M, G 4 ,4/19/99 Tox120, RTL, 63M, G 4 ,4/19/99 Tox120, RBL04199-250ppb-CC3,4/19/99 MeOH Blank . Tox120, RBL04199-H2OBIk-1,4/19/99 Tox120, RBL04199-UvBlk-1,4/19/99 Tox120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-1Oppb-1,4 /1 9 /9 9 Tox120, RBL04199-25ppb-1,4/19/99 Tox120, RBL04199-50ppb-1,4/19/99 Tox120, RBL04199-1Q0ppb-1,4 /1 9 /9 9 ' Tox120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99
MS File
Page 1
Inlet File
long6
long6 long6 longe longe longe long6 longe long6 longe long6 long6 longe longe long6 longe long6 long6 longe long6 long6 long
longe long6 longe long6 long6 long6 long6 long6 longe longe longe longe * longe " long6 long6 longe long6 long6 long6 long6 longe long6
'
Bottle
91 1 2 3 4 5 6 7 8 9 10 11 93 54 55 56 57 58 81 94 59 60 61 62 63 82 94 64 65 66 67 83 94 .1 2 3 4 5 6 7 8 9 10 11
000638
Masslynx - Sample List
i Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\042099d.SPL Printed: Tue Apr 2016:51:401999
1 2 3 4 5 6 7
8 9 10 11 12 13 14 15 16 17 18
19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43
44
File Name
042099106 042099107 042099108 042099109 042099110 042099111 042099112 042099113 042099114 042099115 042099116 042099117 042099118 042099T19 042099120 042099121 042099122 042099123 042099124 042099125 042099126 042099127 042099128 042099129 042099130 042099131 042099132 042099133 042099134 042099135 042099136 042099137 042099138 042099139 042099140 042099141 042099142 042099143 042099144 042099145 042099146 042099147 042099148 042099149
File Text
MeOH Blank Tox120, RBL04199-H20Blk-1,4/19/99 Tox120. RBL04199-UvBlk-1,4/19/99 Tox120, RBL04199-5ppb-1,4/19/99 TOX120, RBL04199-10ppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4/19/99 Tox120, RBL04199-50ppb-1,4 /1 9 /9 9 Tox120, RBL04199-100ppb-1, 4/19/99 Tox120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 TOX120, RBL04199-750ppb-1,4 /1 9 /9 9 Tox120, RBL04199-1000ppb-1,4/19/99 MeOH Blank TOX120, RTL, 49M, G 4 ,4/19/99 Tox120, RTL, 50M, G 4 ,4/19/99 Tox120, RTL, 51M, G 4 ,4/19/99 Tox120, RTL, 52M, G 4 .4/19/99 Tox120, RTL, 53M, G 4 ,4/19/99 TOX120, RBL04199-250ppb-CC1,4 /1 9 /9 9 MeOH Blank Tox120, RTL, 54M, G 4 ,4/19/99. Tox120, RTL, 55M, G 4 ,4/19/99 Tox120, RTL, 56M, G 4 ,4/19/99 Tox120, RTL, 57M, G 4 ,4/19/99 Tox120, RTL, 58M, G 4 ,4/19/99 Tox120, RBL04199-250ppb-CC2,4/19/99 MeOH Blank Tox120, RTL, 59M, G 4 ,4/19/99 Tox120, RTL, 61 M, G 4 ,4/19/99 Tox120, RTL, 62M, G 4 ,4/19/99 Tox120, RTL, 63M, G 4 ,4/19/99 Tox120, RBL04199-250ppb-CC3,4/19/99 MeOH Blank . Tox120, RBL04199-H2OBIk-1,4/19/99 Tox120, RBL04199-UvBlk-1,4/19/99 Tox120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-1Oppb-1,4 /1 9 /9 9 Tox120, RBL04199-25ppb-1,4/19/99 Tox120, RBL04199-50ppb-1,4/19/99 Tox120, RBL04199-100ppb-1,4/19/99 ' Tox120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-100Qppb-1,4 /1 9 /9 9
/K r
Fagel
/ MS File
1 PFBS-4 PFBS-4
PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4
PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 / PFBS-4 . PFBS-4 PFBS-4 I . PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 . PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 i PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4
\
/ /
Inlet File
longe long6 long6 long6 long6 long6 long6 long6 longe longe long6 long long6 long6 long6 longe long6 long6 long
longe long6 long6 long6 long6 Iong6 longe long6 long longe long6 longe long6 long6 long long " long long6 long6 long long long long6 longe long6
Bottle
91 1 2 3 4 5 6 7 8 9 10 11 . 93 54 55 56 57 58 81 94 59 60 61 62 63 82 94 64 65 66 67 83 94 .1 2 3 4 5 6 7 8 9 10 11
000639
S e a m in g M ethod R a p e rt
Method s
C :\MASSLYHX\RATS2. 9RO\ACQUDB\9KBI-COMBX7ZS-PaAA
L a s t `Modified: Toe A pr 20 16*33:12 1099
P rin te d *
Tue A pr 20 16:33*19 1999
Solvent Delay ( nine ) s
0.00
Function * 1
KMC of 1 M a e Pair ( ESP- )
Inter Channel Delay ( Secs ) * Span ( Daltons ) s Start Tine ( Kins ) i Cnd Tine ( Mins ) ; Repeats *
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs)
Coll Energy
(eV)
Cone { V )
1
413.00 169.00
0.30
30
15
Function t 2
MRM of 1 Mass Pair ( ESP- )
Inter Channel Delay ( 5 . ) > Span ( Daltons ) :
Start Time ( Mins ) x End Time ( Mins } i
Repeats * Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Sees)
. Cell Energy
<.V)
Cone
1 469.00 119.00 0.30
30
15
Function : 3
MSN of 1 Mass Pair ( ISP- )
Inter Channel Delay ( Secs ) i Span ( Daltons ) * Start Time ( Mins ) s End Tima ( Mins ) :
Repeats : Channel Parent Dsughtsr
0.03 0.00 0.00 9.00
1 Dwell
(Sees)
Coll Energy
(VI
Cone
1 569.00 119.00 0.30
30
15
Function i 4
MRM of 1 Mass Pair ( ESP- )
Inter Channel Delay ( Secs ) :
Span ( Daltons ) : Start Tine ( Mins ) t
End T i m ( Mins ) : Repeats :
Channel Parent Dsughtsr
0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy U V )
1
419.00 119.00
0.30
30
Cone 15
Function : 5
MRM of 1 M u s Pair ( ESP- )
Inter Channel Delay ( Secs ) * Span { Daltons ) : Start T i m ( Mins ) : End T i m ( Mins ) :
Repeats * Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwsll
(Sacs) Coll Energy (V)
Cone
1
519.00 119.00
0.30
30
15
Function t 6 ' MRM of 1 Muse Pair ( ESP- )
Inter Channel Delay ( Sacs ) : Span ( Daltons ) t
Start T i m ( Mins ) : End T i m ( Mins ) * Repests :
Channel Parent Daughtar
0.03 0.00 0.00 9.00 1 Dell
(Secs) Coll Energy (.V)
Con.
1
499.00 9.00*
0.30
45
SO
Function i 7
MSN of 1 Mase Pair < ISP- )
Inter Channel Delay ( Secs ) i -Span ( Daltons ) :
Start Tiae ( Kina ') s
End Tiae ( Mins ) I Repeats t
Channel Parent Daughter
0.03 0.00 0.00
9.00 1 Dwell
(Secs)
Coll Energy
(eV)
Cone ( V )
399.00 90.00
0.30
45
60
Function : S
MSN of 1 Mass Pair ( ISP- )
Inter charnel Delay ( Secs ) i 0.03
Span ( Daltons )
0.00
Start Tiae ( Mins ) :
0.00
End Tiae ( Mins ) i
9.00
Repeats *
1
Channel Parent Daughter Dwell
(Secs)
Coll Energy
(eV)
Cone ( V )
1
427.00 90.00
0.30
40
90
Page 1
000640
S c a n n in g M athed R e p o rt
Mathed:
C:\WCJSLrNX\RAT92.PWJ\ACCMJ8\9MIM-C0KBma-FaAA
last'Modifiad: Tua Apr 20 10:33:12 199
P rin ta d :
tu a A pr 20 1 0 :3 3 :1 1999
P l 1
Solvant Dalay ( adna ) I
0.00
punetlon : 1
M M of 1 Maaa Pair ( ISP- )
Intar C h a m a l Dairy ( Seca ) t Span ( Daltons ) : Start Tina ( Hina ) End Tina ( Mina ) : Repasts :
Channel Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Saca)
Coll Energy
(V)
Cona ( V )
1
413.00 169.00
0.30
30
15
function i 2
MRM of 1 H u i Pair { ESP- )
Intar Channel Daisy ( Sees ) : Span ( Daltons ) I Start Tine ( Mina ) I
End Tina ( Mina ) : Repeata :
Charnel Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Saca) Coll Energy
(eV)
Cona ( V )
1
409.00 119.00
0.30
30
IS
function t 3
MRM of 1 M m i Pair ( ESP- )
Inter Charnel Delay ( Secs ) x Span ( Daltons ) :
Start Tine ( Mina ) : End Tina ( Mina ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Seca)
Coll Energy
(V)
Cona ( V )
1
509.00 119.00
0.30
30
IS
/
Function : 4
MRM of 1 Mill Pair < ESP- )
Inter Channel Deity ( Seca ) :
Span ( Deltona ) : Start Tina ( Kina ) : End Tina ( Kina ) t
Repeata x Channel Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Seca)
Coll Energy
(V)
Cona ( V )
1
419.00 119.00
0.30
30
IS
Funation 5
MRM of 1 M u i Pair ( ESP- )
Inter Channel Delay ( Seca ) t Span i Daltona ) : Start Tiae ( Mins } i
End Tiae ( Mins ) t Repeata i
Channel Parent Daughter
0.03 0.00 0.00
9.00
1 Dwell
(Seca) Coll Energy
(V)
Cona ( V J
1
519.00 119.00
0.30
30
15
A
Function x * MRM of 1 Maaa Pair ( ESP- )
Inter Channel Delay ( Seca ) t
Span ( Daltona ) : Start Tine ( Mina ) : End Tine ( Mins ) : Repeats s
Channel Parent Daughtar
0.03 0.00 0.00 9.00
1 Dwell
,
(Seca) Coll Energy (V)
Cona ( V )
1
499.00 99.00*
0.30
45
00
Funation x 7
MRM of 1 Mese pair ( BSP- )
Inter Channel Delay { Secs ) x
Span t Daltona 1 s Start Tine ( Mina *) : End Tine ( Mins } i Repeats t
Channel Parent Daughter
0.03 0.00
0.-00 9.00
1 Dwell
(Saca)
Coll Energy
(V)
Cona [ V )
1 - 399.00 80.00
0.30
45
00
Function x 8
MRM of 1 Maas Pair ( ISP- )
Inter Channel Delay ( Sec* ) i
Span | Daltona ) : Start Tine ( Hina ) t
End Tine < Mina ) x Repeats i
Channel Parent Daughter
0.03 0.00 0.00 9.00
l Dwell
(Saca)
Coll Energy
(V)
Cona ( V )
1
427.00 90.00
0.30
40
00
000641
canning Method Xaport
Method:
C:\HASSUna\RAM2.PII0VACaD0B\
LS Modified: Mon Mar 15 14:22:03 1999
P rin te d :
Tua A pr 20 1 7 :0 5 :2 p 1P9P
-4
Solvant Delay ( nina 1 t
0.00
function t X
KRK of 4 K u i Padre ( ISP- )
Inter Channel Delay ( Seca > : Span ( Daltona ) : Start Tina (.Mina )
End Tina ( Mina ) : Rapaata :
Channel Parent Daughter
0.03 0.00 0.00 13.50 1 Dwell
(Saca)
Coll Energy
(eV)
Cone.< V )
1
299.00 '90.00
0.30
2 299.00 99.00 0.30
3
299.00 130.00
0.30
4
299.00 180.00
0.30
45 45
45 45
60 60 60
60
1
T
]
ii
I j
J
J
]
]
I
j
i
'
\
4
Paga 1
n.
000642
AM ELIA062498: FACT E quipm ent L og 99016
Q uattro H , #6448E
PAGE
DATE: 04/22/99
ANALYST: mee STUDY NUM BER Tox-120 M ATRIX: R at L iver D ESCRIPTION: ip
FACT M ethod: ETS-8-5.0________
Injection volum e. IO uL
C ycle tim e: 13.5 m in.
M obile P hase:
(AYLO m M NH4CH3CQ2
TN-A- or stndrd num ber (A ) 99012-5-22_________
Prepared (date/by):
(A) 4/16/99 / ok_________
Expires:
(A ) 10/99________________
Electrode: Z-Spray (B) M eOH (B) TN-A-2414 (B ) 4/9/99 hoi IB ) 10/99________
Store at room tem p? [YJ or N
O ther
G u ard colum n: N one ______________ _
Part number: na_________
L ot num ber: na
A nalytical colum n: keystone betasil____________________________________________
Size: 2 x 50mm__________________ Type: c l 8____________ Sphere size: 5 um
P art number: 055-701-2
L ot num ber: P7L04 Serial num ber 0481172z
A nalytical p aram eters:
t
Drying gas (L/hr): 300
ESI nebulizing gas (L/hr) 15
LC Flow rate: 300 uL/m in. B ack pressure: 59 b ar ''
H PLC solvent g rad ien t: long2
Tim e (m in.) 1) 0.00________ 2) 8.5__________ 3 11.0________ 5) 12.0________ 6) 13.5_________
%B 40 90 90 40 stop
Sheath gas (L/hr) 0
.*s.
000(b43
AM ELIA062498: FACT E quipm ent Log 99016
Q uattro II, #6448E
P A G E ________
DATE: 04/22/99
ANALYST: mee STUDY NUM BER Tox-120 M ATRIX: R at L iver (pfbs) DESCRIPTION: ip
FACT M ethod: ETS-8-5.0
Injection volum e.10 uL
C ycle tune: IS m in. Electrode: Z-Spray
MobUe P hase:
(A12.0 mM NH4CH3CQ2
TN-A- or stndrd num ber (A) 99012-5-22
Prepared (date/by):
(A) 4/16/99 / ok
Expires:
(A ) 10/99
(B) MeOH (B ) TN-A-2414 (B) 4/9/99 hoi (B ) 10/99
Store at room temp? (Y| or N
O ther:
G u ard colum n: None _________
Part number, na
L ot number: na
A nalytical colum n: keystone betasil________________
_________________
Size: 2 x 50mm_________________ Type: c l 8 __________ Sphere size: 5 urn
Part number: 055-701-2
Lot number: P7L04 Serial num ber: 0481172z
A nalytical param eters:
Drying gas (L/hr): 300
ESI nebulizing gas (L/hr) 15
LC Flow rate: 300 uL/m in. B ackpressure: 59 bar
Sheath gas (L/hr) 0
H PLC solvent g rad ien t: long2
Time (m in.) 1) 0.00________ 2) 5.0__________ . 3 12.0 51 13.5 6) 15_________
%B 25 95 95 25 stop
000644
/^ fZ F
Tuning Method Report
Method:
C:\MASSLYNX\RATS2.PR0\ACQUDB\PF0S
Printed:
Thu Apr 22 16:25:24 1999
AimL OLiHHf
Page 1
LAC, Of/l/M
BESTCOPYAVAILABLE
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 15 5 0.2
150
250
2.96 -14 -5 -
152 253
Pressures
Analyser Vacuum Gas Cell
Rdbk
2. Oe-4 5 . 5e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0 2 0 0.0 . 58 650
Set Rdbk
15.0 15.0
30 2.0 0.0 40 0 650
32
39 31
000645
Masslynx - Sample List
Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\042299a.SPL
Printed: Thu Apr 2217:50:31 1999
^
y/*<;?7
Page 1
File Name
File Text
1 042299001
MeOH Blank
2 042299002
Tox120, RBL04199-H 20Blk-1,4 /1 9 /9 9
3 042299003
TOX120. RBL04199-LivB!k-1,4/19/99
4 042299004
Tox120, RBL04199-5ppb-1,4/19/99
5 042299005
TOX120, RBL04199-10ppb-1,4/19/99
6 042299006
OX120, RBL04199-25ppb-1,4/19/99
7 042299007
TOX120, RBL04199-50ppb-1,4 /1 9 /9 9
8 042299008
TOX120, RBL04199-1 OOppb-1,4/19/99
9 042299009
Tox120. RBL04199-250ppb-1,4 /1 9 /9 9
10 042299010
TOX120, RBL04199-500ppb-1,4 /1 9 /9 9
11 042299011
TOX120, RBL04199-750ppb-1,4 /1 9 /9 9
12 042299012
Tox120, RBL04199-1000ppb-1,4/19/99
13 042299013
MeOH Blank
14 042299014
TOX120, RTL, 49M, G 4 ,4/19/99, D10
15 042299015
TOX120, RTL, 50M, G 4 ,4/19/99, D10
16 042299016
TOX120, RTL, 51M, G 4 ,4/19/99, D10
17 042299017
Tox120, RTL, 52M, G 4 ,4/19/99, D10
18 042299018
Tox120, RTL, 53M, G 4 ,4/19/99, D10
19 0422990-19
TOX120, RBL04199-250ppb-CC1,4 /1 9 /9 9 .
20 042299020
MeOH Blank
21 042299021
TOX120, RTL, 54M, G 4 ,4/19/99, D10
22 042299022
TOX120, RTL, 55M, G 4 ,4/19/99, D10
23 042299023
Tox120, RTL, 56M, G 4 ,4/19/99, D10
24 042299024
Tox120, RTL, 57M, G 4 ,4/19/99,
25 042299025
TOX120, RTL, 58M, G 4 ,4/19/99,
26 042299026
TOX120, RBL04199-250ppb-CC2,4/19/99
27 042299027
MeOH Blank
28 042299028
Tox120, RTL, 59M, G 4 ,4/19/99,
29 042299029
TOX120, RTL, 61 M, G 4 ,4/19/99,
30 042299030
TOX120, RTL, 62M, G 4 ,4/19/99, .
31 042299031
TOX120, RTL, 63M, G 4 ,4/19/99,
32 042299032
TOX120, RB L04199-250ppb-CC1,4 /1 9 /9 9
33 042299033
MeOH Blank
34 042299034
TOX120, RBL04199-H2OBIk-1,4/19/99
35 042299035'
TOX120, RBL04199-LlvBlk-1,4 /1 9 /9 9
36 042299036
Tox120, RBL04199-5ppb-1,4/19/99
37 042299037
TOX120, RBL04199-1 Oppb-1,4 /1 9 /9 9
38 042299038 . TOX120, RBL04199-25ppb-1,4/19/99
39 042299039
T0 X I2 O, RBL04199-50ppb-1,4/19/99
40 042299040
TOX120, RBL04199-100ppb-1,4/19/99
41 042299041
TOX120, RBL04199-250ppb-1,4/19/99
42 042299042
TOX120, RBL04199-500ppb-1,4 /1 9 /9 9
43 042299043
Tox120, RBL04199-750ppb-1,4/19/99
44 042299044
TOX120, RBL04199-1000ppb-1,4/19/99
>
MS File
15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15M1N 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15M1N 15MIN 15MIN 15MIN
Inlet R e
long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 * long15 long15 long15 long15 long15 long15 long15 long15 long15 long15
Bottle
91 1
2 3
4 5 6 7 8 9 10 11 91 12 13 14 15 16 17 91 18 19 20 21 22 23 91 24 25 26 27 17 92 1 .. 2 3 4 5 6 7 8 9 10 11
000646
VA*Jw
Masslynx - Sample List
Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\042299b.SPL Printed: Thu A p r2217:46:29 1999 ____________ / W i f f l . b L M H #
I A C 6.ilh d tf .
Page 1
File Name
File Text
1 042299045
MeOH Blank
2 042299046
Tox120, RBL04199-H20B!k-1,4/19/99
3 042299047
Tox120, RBL04199-LivBlk-1,4/19/99
4 042299048
Tox120, RBL04199-5ppb-1,4/19/99
5 042299049 . Tox120, RBL04199-10ppb-1,4/19/99
6 042299050
Tox120, RBL04199-25ppb-1,4/19/99
7 042299051
Tox120, RBL04199-50ppb-1.4/19/99
8 042299052
TOX120. RBL04199-100ppb-1,4 /1 9 /9 9
9 042299053
Tox120, RBL04199-250ppb-1, 4/19/99
10 042299054
Tox120, RBL04199-500ppb-1,4/19/99
11 042299055
Tox120, RBL04199-750ppb-1,4/19/99
12 042299056
Tox120, RBL04199-1000ppb-1,4/19/99
13 042299057
MeOH Blank
14 042299058
Tox120, RTL, 17M, G 2 ,4/19/99
15 042299059
Tox120, RTL, 18M, G 2 ,4/19/99
16 042299060
Tox120, RTL, 19M, G 2 ,4/19/99
17 042299061
Tox120, RTL. 20M, G 2 ,4/19/99
18 042299062
Tox120, RTL. 21M, G2. 4/19/99
19 042299063
Tox120, RBL04199-250ppb-CC4,4/19/99 .
20 042299064
MeOH Blank
21 042299065
Tox120, RTL, 22M, G 2 .4/19/99
22 042299066
Tox120, RTL, 23M, G 2 ,4/19/99
23 042299067
Tox120, RTL, 24M, G 2 ,4/19/99
24 042299068
Tox120, RTL, 25M, G 2 ,4/19/99
25 042299069
Tox120, RTL, 26M, G 2 ,4/19/99
26 042299070
Tox120, RBL04199-250ppb-CC5,4/19/99
27 042299071
MeOH Blank
28 042299072
Tox120, RTL, 27M, G 2 .4/19/99
29 042299073
Tox120, RTL, 29M, G 2 ,4/19/99
30 042299074
Tox120, RTL, 30M, G 2 ,4/19/99
31 042299075
Tox120, RTL, 31 M, G 2 ,4/19/99
32 042299076
Tox120, RBL04199-250ppb-CC6,4/19/99
33 042299077
MeOH Blank
34 042299078
Tox120, RTL, 33M, G 3 ,4/19/99
35 042299079
Tox120, RTL, 34M, G 3 ,4/19/99
36 042299080
Tox120, RTL, 35M, G 3 ,4/19/99
37 042299081
Tox120, RTL, 36M, G 3 .4/19/99
38 042299082
Tox120, RTL, 37M, G 3 ,4/19/99
39 042299083
Tox120, RBL04199-250ppb-CC7,4/19/99
40 042299084
MeOH Blank
41 042299085
Tox120, RTL, 38M, G 3 ,4/19/99
42 042299086
Tox120, RTL, 39M, G 3 ,4/19/99
43 042299087
Tox120, RTL, 40M, G 3 ,4/19/99
44 042299088
TOX120, RTL, 41M. G 3 .4/19/99
45 042299089
Tox120, RTL, 42M, G 3 ,4/19/99
46 042299090
Tox120, RBL04199-250ppb-CC 8,4/19/99
47 042299091
MeOH Blank
48 042299092
Tox120, RTL, 43M, G 3 ,4/19/99
49 042299093
Tox120, RTL, 45M, G 3 ,4/19/99
50 042299094
Tox120, RTL, 46M, G 3 ,4/19/99
51 042299095
Tox120, RTL, 47M, G 3 ,4/19/99
52 042299096
Tox120, RBL04199-250ppb-CC9, 4/19/99
53 042299097
MeOH Blank
54 042299098
Tox120, RB L04199-H20Blk-1, 4/19/99
55 042299099
Tox120, RBL04199-LivBIk-1, 4/19/99
56 042299100
Tox120, RBL04199-5ppb-1,4/19/99
57 042299101
Tox120, RBL04199-1 Oppb-1,4 /1 9 /9 9
58 042299102
Tox120, RBL04199-25ppb-1, 4/19/99
59 042299103
Tox120, RB L04199-50ppb-1, 4/19/99
60 042299104
Tox120, RBL04199-100ppb-1, 4/19/99
MS File
Inlet File
13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13M IN-COM BI7IS-POM 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13M IN-COMB I7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13M IN -C O M B I7IS -P O M 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13M IN-COMB I7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI71S-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13 M IN -C O M B I7 IS -P O A A 13 M IN -C O M B I7 IS -P O A A 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13MIN-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 13 M IN -C O M B I7 IS -P O A A
long2 long2 long2 long2 long2 long2 long2 long2 long2 long2
long2 long2 long2 long2 long2 long2 long2 long2 tong2 long2 long2 long2 long2 long2
long2 long2 long2 long2 long2 long2 long2 long2 long2 Iong2 long2 long2
long2 long2 long2 long2 long2 long2 long2 long2 long2 long2
long2 long2
long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2
Bottle
91 1 2 3 4 5 6 7 8 9 10 11 91 31 32 33 34 35 84 92 36 37 38 39 40 85 92 41 42 43 44 86 93 45 46 47 48 49 87 93 50 51 52 53 54 88 93 55 56 57 58 89 93 1 2 3 4 5 6 7
000647
Masslynx * Sample List
Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\042299b.SPL / Printed: Thu Apr 2217:46:291999______________________ A m l i C .
File Name
61 042299105 62 042299106 63 042299107 64 042299108 65 66 67 68
70 71 72 73 74
File Text
Tox120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 TOX120, RBL04199-750ppb-1,4 /1 9 /9 9 Tox120, RBL04199-1000ppb-1,4/19/99
y /* < ./? y
Page 2 /, I A D / Z o / ^ _______________
M S File
13M1N-COMBI7IS-POAA 13M1N-COMBI7IS-POAA 13 M IN -C O M B I7 IS -P O A A 1 3 M IN -C O M B I7 IS -P O A A
Inlet File
long2 long2 long2 long2
Bottle
8 9 10 11
000648
V /^ v 9 /
c a n n in g M ethod R a p o rt
Mathod:
C!\MASSLWX\ItATSa.P0\ACQUDB\13MXH-CCt<BI7I-PQAA
taut Modified: Thu Apr 22 1C:S0:10 1999
Printed:
Thu Apr 22 1:30:1( 1999
A p v L ____n u x i V
Solvant Delay ( nine ) i
0.00
Punction : X
to o f 1 Mfeaa fair { ESP- )
Inter Channel Delay ( Seca 1 t Span ( Daltons I ; Start Tine ( Kins 1 : End Tine ( Kins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell
(S.ca) Coll Energy (eV)
* Co m ( V )
1 413.00 169.00 0.30
30
15
function : 2
MRM of 1 Maes fair I ESP- )
Inter Channel Delay ( Saca ) : Span ( Daltons ) : Start Time | Hina ) !
End Tine l Mins ) :
Repeats : Channel Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell
,
(Saca) Coll Energy
(aV)
1 469.00 119.00 0.30
45
*
Cone C V ) 33
function : 3
MRM of 1 Haai Pair ( ESP- )
Inter Channel Delay ( Saca ) : Span ( Daltons ) :
Start Time ( Mina ) : End Tine ( Mins ) : Repeats i
Channel Parent Daughter
0.03 0.00 0.00 13.50 1 Dwell
(Saca)
Coll Energy
(eV)
COM t V >
1 569.00 119.00 0.30
45
35
function : 4
MRM of 1 Mass Pair t ESP- )
Inter Channel Delay ( S.ca ) i
Span ( Daltons ) : Start Tine ( Mins ) : End Tine ( Mins ) s Repeats t
Channel Parent Daughter
0.03 0.00 0.00 13.50 1 Dwell (Saca) Coll Energy (aV)
.
Cone ( V )
1 419.00 119.00 0.30
45
35
function : 9
MRM of 1 Miss fair { ESP- )
Inter Channel Delay ( S.ca ) :
Span ( Daltons ) :
Start Tine { Mina ) :
End Tim. ( Mina ) :
Repeats :
Channel Parent Daughter
0.03 0.00
0.00 13.50
1 Dwell
(Secs)
Coll Energy
(eV)
Cone ( V )
1
519.00 119.00
0.30
45
35
Function : ( ' MRM of 1 Maaa Fair { ESP- >
Inter Channel Delay ( Saca ) Span ( Daltons ) : Start Tine { Mins ) : End Tine J Mins ) ;
Repeats :
Channel Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell (S.ca) Coll Energy (V)
Cano ( V )
1
499.00 99.00*
0.30
45
<0
function t 7
MRM of 1 Maas fair ( ESP- )
Inter Channel Delay ( Secs ) i Span ( Daltona ) Start Tim* ( Mina ) i End Tine ( Mina ) t Repeats s
Channel Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell
(Secs)
Coll Energy
(eV)
1
399.00 60.00
0.30
45
Cone I V 1 <0
Function : S
MRM of 1 Maaa fair ( ESP- )
Intar Channel Delay ( Secs ) ; Span ( Daltons ) : Start Tine { Mins ) s End Tine ( Mins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell
(Secs)
Coll Energy
(V)
1
427.00 80.00
0.30
40
Cone ( V ) 60
LAc- o i L h t
.t*
000649
P ig t 1
S c a n n in g M ethod R e p o rt
Method:
c:\MMSLnoc\iuTS2.MoVAOQOoaUSK
Last Modifiad: Thu Apr 32 13:32:50 1999
Printed:
Thu Apr 22 13:52:34 1999
Solvent Dlay < nine > >
0.00
function : 1
M M a t 4 Mass fairs ( U f - 1
Inter Channel Delay (1 Sac* ) i Span ( Daltons ) : Start Tine ( Kina ) i End Tine ( Mine J : Repeats s
Channel Parent Daughter
9.03 0.00 0.00 IS.00 1
Etoell (Secs)
1
299.00 90.00
0.30
2
299.00 99.00
0.30
a
299.00 130.00
0.30
4
299.00 180.00
0.30
45 45 45 45
1
i
I sH*
A n u L /LVIV Ucox/io/w
Cons | V J 60 60 60 60
fag s 1
000650
S c a n n in g M ethod R e p o rt
Hathod:
C: \ M M a L 0 ( \ R X I S .P0\*CQUI!B\1
Last Modified: Ihn Apr 22 19:92:90 1*
Prlntad:
Thu Apr 22 19:92:94 199
________ ri rmr i ttt iLfj n h W
Solvant Palay ( atina )i i
0.00
function : 1
m M of 4 Haas falsa ( IIP- >
Intar Charmai Palay ( Sacs ) : Span ( Daltons ) : Start Tiaa ( Hina ) : End Tina { Kina ) : Riputa :
Channel Parant Daughtar
0.03 0.00
0 a00 19.00
1 thrall (Secs) Coll Energy (aV)
Cons I V )
1
299.00 90.00
0.30
2
299.00 99.00
0.3
3
299.00 130.00
0.30
4
299.00 190.00
0.30
45 45 45
45
60 60
60 60
* //* * /? ?
Pag. i
U t O j/z o /w
er
000651
] FACT: DATA REVIEW SUMMARY FORM
Date:
4 /2 3 /0 9
] Analyst:
L
FACT Study Number 1 D X - 1 ^ 0
I Matrix
\A a d jL U ' w f
Urne Points
1 Dilutions?
tffu p H
S(/mc oaJ -
3 Analyte 1:
fNr..p...b...s^'i
Acceptable LinearRange: 1
ipnplo
LOQ fiartills data set:
1 Reevahuted&r differentname?
fvo
Analyte 4: Acceptable Linear Ranjg: LOQ for this data set Reevaluated for different
Analyte 5: Acceptable] LOQfor tbia data set
Lfor dxffbicQ-trange?
Analyte2:
Acceptable LinearRango:
LOQ far this data set
] Reevaluated for different range?
J Analyte3; Acceptable Linear Range:
LOQ fiartills data set 3 Reevaluated for differentrange?,
z
A j l y t e 6:
Acceptablelinear Range: A \ LOQ for this data set
Reevaluated for differentrange?
Analyte 7: Acceptable T Range: LOQfor tide data set: Reevaluated far different range?
]
DinJfilU potk* * dkAJXKSi- j \oof*. p*-ab* i'oprJr*d-
i
V U '5
o.
(yu * f / 2 - 5 / d 3
J
] 000652
i
FACT: DATA REVIEW SUMMARY FORM
Date:
Analyst: FACT Study Number
JL
T V X 'IU )
Matrix Tima Pointa Dilutions?
h a lt u m v s
m c p y-
SU Y ru. yy X~
Analyte 1: Acceptable LinearRange: 5 '^ 7'S D lr> jylo
LOQ for ds data set: Re-evahiated far differentrange?
oo
Analyte 2: Acceptable LinearRange: LOQ for this data set Re-evaluated for differentrange?
Analyte 3; Acceptable Linear Rango^ LOQ for this data set Re-evaluated for differentrange?
Analyte 4: Acceptable Linear Range: LOQ for this data set: Re-evaluated for different
Analyte 5: Acceptable LOQ for this i&ta set:
Acceptable Linear Range: LOQ for this data set Re-evaluated for differentrange?
Analvte7: Acceotable Linear Range: LOOfor this data set Re-evaluated for different range?
X )o J fiU p u d * -*'* \} M S
O -.
fcp.
.
000653
Vi
o
1
Vt0VO*Bil)iO
HHN I
I CM
III
t
SS522SSSSSisSliS5SS55SiSSS3SSSS2S:8SSSS
)nH rn >*ioHrv ri A* r'oo'n no on iHv h< ,St Hn Hi rn'B*ODrr4f ) Di fw lfrtaw*snoMin >9 )r a noviNnnSHM Hn t oN <anenr*NrHH n
HrHt N h s NMSS^HB Pv tvnNvSnO N Hi oBfO BH elfNtrN'Oi Nn vhoiflfftHN Bn vNotHo if
tnviABMn^oooA
h n o n n rt
f N l O ^ rr t rt r* n w kjh n h
t
h t * nr* r*
>nnmnMSSiNnw^pS*f^nSnBaCInmSoS^tS'SoS~lorovthOfH*Atow^rHvrSnVrtOS-OMSr-e'moi-D<Shrf l a w m a h v D f - n n o N h HNnHNV H W
w n HHi tT0i N H H H r *
H N D H h hHn Ht
J^AOMNxAAnwAaAArnrtA"AntAnf'*tn*nio,nooxnff*'imerW*nNrnWA^Nrr'N*AAiMXniHr-lM*NrrNrNr'*iNo*'B0f'*oi^nnow'AnOMo^ inr-frnl*ran%NrANfo,>fsAn*n0nMsn,aAnanrsn*sAn fsn-snA
lo n o o o o o o HNinomomo -tenor o
litllllif
M oweooooo HMSoaomo HNlflhO
ikhhhN M M h
ilfiillil
Last nodifitdi ftl Apr 23 08:42:43 1999
C:\MfcS9LTOC\rata2.FRO\MathDB\042299a
Last nodlfiad: M Apr 23 08:51:2 199
arpia List: C :\MfcSSLYXX\rt*2.PRO\SawplaDB\042299a
.NOAkAASafAtAAAAASANAAANAANA'sA.
AAs
ooooos H H H H pt A QOOQQh
ooo H H p< QQ
NHAANHANA'AH>AH.NAH.HASANHANrtAHNiHS^
SAHNAHSAr-tSpA1.SAHUO
AAAAAAAA
sAAHAsAAHAaAN'asA.a-Af^tA'AAs.AAHsAAAsHAAsAHAAsAHAAV-V.
PV W
NAH SHASHASHA.UHU
.H H HH I
I I .HH H I I I I A
3 3VAAS||S
ghfhaasosmao&oAUoSmso
V.^. X.1.^
^*Qi
.. ^ r
.
.. r ^
a o
oo u o litn u o u u o m
o>ftftftftoooooN N^ S S AI
I I .H H H I M M r * l I I 4343A._ as
* A* n o i l 'S a 'S. f t'Sa-Sa ' f to
^^ ^o ooeum
ftonooinom o
SdflHN ArtN flr>H
BZU
ooooooooooo %%o ..*;..O ***JO oooooocAI AI AI AI AI AI Ai Al AIAI AI
AAAAAAAAAAA
SS5SSS
.I &388
tt
AAAAAAAAAAA
oooo
1*4 J 1a
|^S2SSSS2SaSS3SEa|SSS3S|SaS2S2! 2222
u
SrlX"ofaHXtn*OoHXn--O-o-HXn-O-o--HXn-O-o-H-Xn-O-o*HXnO*oHnX*OoHnXiOoHInXOpHnXOonXOoOAaXnOoXnOoXOoXnOoXnOo XOo OA_a XOno XiO*onXO*onXO*onXOw*o XOo.n...O.A._a....XO.o....XO.om....O.o.X...O.oX.n..OonXAOOp*nXOpnHXOpnHXOnpHXnOoHXmOoHXONHXo HONXo HONoXHONoXHOnoX
ooeo3INNHoAoAWNHNAAoo NN-NAoAo.MHNAAoo.NN<ANooANNnNNXAAoowNNNNAooAr>NNNNAAooNNNXAoAAoNNAANXOWNMNAAONNAANNOWNNOAAMNNAnBoaNHNNtnNAeaHNlNMonAaHNvMnoAaNHNrnoANaHWBnoAManHMnoAAONNNnoHAANNNnnoAaPNNNrnloAaNNiNnAoANvNNnAoaNBNNnAoaWNNNnAoMaNNNniAoaNNNBnAoaNNONnAoaNNinnAoafNNirlnAoatNNWNnwaAoNnnNNnAANoWHCAAorMiWNNAANpoNNi[NNAAon'NNNAoAni>IINN(fnOnAAN<0<rWNoAOVNAHANANONNANOANNAA^On--NNOA^At
S Ho No no o so o No so AoOoHoNonoio o o o o o o o o o o o o o o o o o o o o o o o o o o
Job Coda:
Hsthod:
U
CD
O
O
O
A m i l a 042498; Raviawad and quanti tatad by kjh 4/23/99
Quantify Calibration Raport
Calibration: C:\HAS3Un0C\rata2.PRO\CurvaDB\042299a
Last aodifiad: Fri Apr 23 08:31:34 1999
Printad:
Pri Apr 23 08:32:40 1999
'
Pag 1
Amalia 082498: Raviawad and quantitatad by kjh 4/23/99
000655
000656
1
;p
O e
$ ?
1*0 -t
Mt (titHH er H tu
`tNnttMa<(i*NS'<>tn tiM i ii ** ti{M i\u
wn
itvi nm m
SOUP 020199: FACT Equipm ent L og 99014
Q uattro II, #6414E
PAGE
DATE: 05/14/99
A N A L Y S T idrb
STUDY NUMBER: T ox-120 M ATRIX: R at Liver DESCRIPTION: dilutions, FACT M ethod: ETS-8-S.0 .
Injection volume: 10 uL
Cycle tim e: 9 min. Electrode: Z-Spr^y---------
M obile P hase:
(A12.0 mM NH4CH3CQ2
(B)M eQ H ---------
TN-A- or stndrd num ber (A) 99011-6-9__________
(B) TN-A-25.Q6
Prepared (date/by):
fAl 04/28/99 drb______ _
(B) Q4/26/99,drb
Expires:
(A1 10/99_____________ __
(B ).,10/99_____
Store at room temp? [y| or N
Other:---------------------------- .--------
G u ard colum n: None______________
P art number: na_________
Lot num ber:jna_______________________
A nalytical colum n: Keystone B etasil __________________________________
Size: 2 X 50 mm
___________ Type:j18____________ Sphere size:J u ffi______
Part number: 055-701-2__________ L ot num ber P7LQ4
Serial numben0481175Z
A nalytical param eters:
Drying gas (L /hrl: 350
ESI nebulizing gas (L/hrl 15
LC Flow rate: 3 0 0 11T./min. B ack pressure: 55 bar
Sheath gas (L/hr)^.
H PLC solvent gradient: 9m in-2
Time (min.)
D0.00______ 2) 1.00______
3) jL5Q____
416.50 51 7.00______
%B_
40.0 40.0
J10
35SL 40.0_
000657
Tuning Method Report
Method:
C :\MASSLYNX\KACT-1.PRO\ACQUDB\PFOS
Printed:
Pri May 14 16:17:39 1999
Rage 1
.EST COPY AVAILABLE
MS
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 60 5 0.2 150 250
2.98 -29 -7
153 248
Pressures
Analyser Vacuum Gas Cell
Rdbk
3.3e-5 4 .3e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
'.*X
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
14.0 14.0 * 2.0 . 0.0 56 650
Set Rdbk
15.0
15.0 45 2.0 0.0 40
0 650
42
40 44
000658
Masslynx - Sample List
Sample Ust: C:\MASSLYNX\FACT-1.PRO\SampleDB\05T499c.SPL Printed: Fri May 1416:17:21 1999
Page 1
File Nami File Text
MS R ie Inlet I Botti ilume Sample T Cone A
1 05149906 MeOH Blank 2 05149906 MeOH Blank 3 05149906 Tox-120, RBL04199-H20Blk-2,4/19/99 4 05149906 Tox-120, RBL04199-LBIk-2,4/19/99 5 05149906 Tox-120, RBL04199-5ppb-2,4/19/99 6 05149906 Tox-120, RBL04199-10ppb-2, 4/19/99 7 05149906 Tox-120, RBL04199-25ppb-2,4/19/99 8 05149906 Tox-120, RBL04199-50ppb-2, 4/19/99 9 05149906 Tox-120, RBL04199-1 OOppb-2,4/19/99 10 05149907 Tox-120, RBL04199-250ppb-2,4/19/99 11 05149907 Tox-120, RBL04199-500ppb-2,4/19/99 12 05149907 Tox-120, RBL04199-750ppb-2,4/19/99 13 05149907 Tox-120, RBL04199-1000ppb-2, 4/19/99 14 05149907 MeOH Blank 15 05149907 Tox-120 23M G 2/D 1:25 16 05149907 Tox-120 24M G2 / D1:25 17 05149907 Tox-120 25M G2 / D1:25 18 05149907 Tox-120 26M G 2/D 1:25 19 05149907 Tox-120, RBL04199-250ppb-CC1, 4/19/99 20 05149908 MeOH Blank 21 05149906 Tox-120 33M G 3 /D 1 2 5 22 05149908 Tox-120 34M G3/D1 5 23 05149908 Tox-120 35M G 3/D 1:25 24 05149908 Tox-120 36M G 3/D 1:25 25 05149908 Tox-120 37M G 3/D 1:25 26 05149908 Tox-120 38M G 3 /D 1 2 5 27 05149908 Tox-120 39M G 3/D 1:25 28 05149908 Tox-120 40M G 3/D 1:25 29 05149908 Tox-120, RBL04199-250ppb-CC3,4/19/99 30 05149909 MeOH Blank 31 05149909 Tox-120 2 7 M G 2 /D 1 :1 0 32 05149909 Tox-120 2 9 M G 2/D 1:10 33 05149909 Tox-120 30M G 2/D T.10 34 05149909 Tox-120 31 M G 2 /D 1 :1 0 35 05149909 Tox-120, RBL04199-250ppb-CC4, 4/19/99 36 05149909 MeOH Blank 37 05149909 Tox-120 4 2 M G 3/D 1:10 38 05149909 Tox-120 43M G 3/D 1:10 39 05149909 Tox-120 45M G 3/D 1:10 40 05149910 Tox-120 46M G 3/D 1:10 ' 41 05149910 Tox-120 4 7 M G 3 /D 1 :1 0 42 05149910 Tox-120, RBLQ4199-250ppb-CC5, 4/19/99
43 05149910 MeOH Blank 44 05149910 Tox-120, RBL04199-H20Blk-2, 4/19/99 45 05149910 Tox-120, RBLQ4199-LBIk-2,4/19/99 46 05149910 Tox-120, RBL04199-5ppb-2,4/19/99 47 05149910 Tox-120, RBL04199-1 Oppb-2,4/19/99 48 05149910 Tox-120, RBL04199-25ppb-2,4/19/99 49 05149910 Tox-120, RBL04199-50ppb-2,4/19/99 50 05149911 Tox-120, RBL04199-1 OOppb-2,4/19/99 51 05149911 Tox-120. RBL04199-250ppb-2,4/19/99 52 05149911 Tox-120, RBL04199-500ppb-2,4/19/99 53 05149911 Tox-120, RBL04199-750ppb-2,4/19/99 54 05149911 Tox-120, RBL04199-1000ppb-2,4/19/99
TELMER 9min- 96
10 Analyte
TELMER 9min- 96
10 Analyte
TELMER 9min- 41
10 Analyte
TELMER 9min- 42
10 Analyte
TELMER 9min- 43
10 Standard 4.93
TELMER 9min- 44
10 Standard 9.76
' TELMER 9min- 45
10 Standard 24.8
TELMER 9min- 46
10 Standard 49.3
TELMER 9min- 47
10 Standard 97.6
TELMER 9min- 48
10 Standard 248
TELMER 9min- 49
10 Standard 493
TELMER 9min- 50
10 Standard 735
TELMER 9min- 51
10 Standard 976
TELMER 9min- 96
10 Analyte
TELMER 9min- 52
10 Analyte
TELMER 9min- 53
10 Analyte
TELMER 9min- 54
10 Analyte
TELMER 9min- 55
10 Analyte
TELMER 9min- 56
10 Analyte
TELMER 9min- 96 .
10 Analyte
TELMER 9min- 57
10 Analyte
TELMER 9min- 58
10 Analyte
TELMER 9min- 59
10 Analyte
TELMER 9min- 60
10 Analyte
TELMER 9min- 61
10 Analyte
TELMER 9min- 62
10 Analyte
TELMER 9min- 63
10 Analyte
TELMER 9min- 64
10 Analyte
TELMER 9min- 65
10 Analyte
TELMER 9min- 97
10 Analyte
TELMER 9min- 66
10 Analyte
TELMER 9min- 67
10 Analyte
TELMER 9min- 68
10 Analyte
TELMER 9min- 69
10 Analyte
TELMER 9min- 70
10 Analyte
TELMER 9min- 97
10 Analyte
TELMER 9min- 71
10 Analyte
TELMER 9min- 72 ' 10 Analyte
TELMER 9min- 73
10 Analyte
TELMER 9min- 74
10 Analyte
TELMER 9min- 75 x
10 Analyte
TELMER 9min- 76
10 Analyte
TELMER 9min- 98
10 Analyte
TELMER 9min- 41
10 Analyte
TELMER 9min- 42
10 Analyte
TELMER 9min- 43
10 Standard 4.93
TELMER 9min- 44
10 Standard 9.76
TELMER 9min- 45
10 Standard 24.8
TELMER 9min- 46
10 Standard 49.3
TELMER 9min- 47
10 Standard 97.6
TELMER 9min- 48
10 Standard 248
TELMER 9mln- 49
10 Standard 493
TELMER 9min- 50 TELMER 9min- 51
10 Standard 735 10 Standard 976
' Factor 1
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
000659
Scanning Mathod Report
Method:
C: \HXS8LniX\FACT-l. PR0UCQODS\TSXJaai9
Last Modified: frl M ay 14 15:30 01 1999
Printed:
frl May 14 18:17x28 1999
olrent Daisy ( alas ) i
0.00
Function s 1
MUM of 3 Maas fairs ( U > - )
Inter Chaaasl Daisy ( Sacs J i Spaa ( Daltons ) :
Start Tina ( Kins ) :
Bad Tina ( Kins ) : Repeat i
Channel Parent Daughter
0.03 0.00 0.00 9.00
1
(Sacs) Coll B nergy (aV)
Cona ( V )
HH
1
1
413.00 119.00
0.2S
2
413.00 169.00
0.25
3
413.00 219.00
0.25
30
20 30
15 IS 15
Function s 2
M M of 1* Mass Pair ( - )
.
Inter Channel Delay ( Secs } t Spaa ( Daltons ) i Start Tina ( Mins ) i Snd Tina ( Mins ) i Repasts t
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Sm s ) Coll
Bnergy
(aV)
Cone ( V )
1
463.00 219.00
0.40
20
35
nineties : 2
MRK of 1 Mass Pair ( >
Inter Channel Delay ( Secs ) : Span ( Daltons ) t Start Tina ( Kins ) i Bad Tine ( Kins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell (Sacs) Coll Bnergy (aV)
Cone ( V )
1
513.00 219.00
0.40
20
35
Function x 4
KKK of 1 Mass Pair ( BSP- )
Inter Channel Delay ( Secs ) : Span ( Daltons ) i Start Tina ( Mins ) i End Tine ( Mins ) : Repeats t
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell (Sacs) Coll Bnergy (aV)
Cone ( V )
1
563.00 219.00
0.40
25
30
Function s S
HUM of 1 Mass Fair ( BSP- )
Inter Channel Delay ( Secs ) i Span ( Daltons ) i Start Tine ( Mins ) i Xnd Tine ( Mins ) i Repeats i
Channel Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell (Sacs) Coll Bnergy (aV)
Cone ( V )
1
13.00 219.00
0.40
20
40
function t *
mat of 1 Mans falx ( BSP- )
Inter Channel Delay ( Sees ) : Span ( Daltons ) : Start Tine ( Kins ) : Snd Tine ( Mias ) i Repeats :
Channel Parent Daughter
0.03 0.00
0.00 9.00
1 Dwell
(Sacs)
Coll Bnergy
(aV)
Cone ( V )
1
427.00 80.00
0.40
40
60
Vl0* 1
000660
FACT: DATA REVIEW SUMMARY FORM
Date: fls//e/Ti
Analyst: FACT Study Number
I
Matrix
Time Points Dilutions? Analyte 1:
' & + Z 3 ...
|:35~J|'Jl/h).
PoA A
Acceptable LinearRange:
,
LOQ fix this data set
/ >
Re-evaluated fix diffisent range?
.
Analyte 4:
yl/1 S & 3
Acceptable Linear Range: --A ) 0 S T A jO- --
LOQ for this data set:
*
Re-evaluated for different range? -- .
AnafyteS:
/>?,/3
AcceptableLipearRange:
LOQ for this data set
Re-evaluated for differentrange?
Analyte 2: Acceptable T.inearRange: -- LOQ for this data set __________ ' Re-evaluated far different range?
Analyte 3:
TW
AcceptableT.incarRange:
LOQ for this data set
/ P j t f c --------------
Re-evaluated for different range?
^ fo r /W V & 3
Lw I
(k .'fC ' A - b & y * -
(
H
V l. * 4 ^ 3
^ A1563 JkiuUxis't*
W f -V k , leA*H--hrv^JL fro - + U -
d -b d v ^ -
ftsVis/ft
/ " * / . v a . c w fe
S '"4 /) / 'Jo
D /;3S "d-0/M O
000661
cfe
Q u a n tify Com pound Sum mary R a p o rt TOX-120 R a t L lv a r D ilu tio n
Saltila U a t : S:\rACT-l.FRO\SaaplaDB\OS1499a
Last modifind: Mon May 17 14:14:20 1999
M a t h od:
S:\rxCS-l.FMO\MathDB\051499e
Laat modlfiad: Mon May 17 14:23:38 1999
Job Coda:
Frintad:
Tua May II 09:22:40 1999
Compound 1: PCAX
Maaa 1 051499081 2 051499082 3 051499083
4 051499064 5 051499065 6 051499066 7 051499067
S 051499068
9 051499069 10 051499070 11 051499071 12 051499072 13 051499073
14 051499074
15 051499075 16 051499076
17 051499077
18 051499078 19 051499079
20 051499080
21 051499081 22 051499082 23 051499083 24 051499084 25 051499085
26 051499086
27 051499087
26 051499088
29 051499089
30 051499090 31 051499091
32 051499092
33 051499093
34 051499094
35 051499095 36 051499096 37 051499097
38 051499098 39 051499099 40 051499100
41 051499101 42 051499102 43 0S1499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 SO 051499110 51 051499111 52 051499112 53 051499113
54 051499114
Sairplaliat Taxt
MaOH Blank
MOH Blank
Tox-120, RBL04199-B20Blk-2, 4/19/99
Tox-120, *Bl04199-tSlk-2, 4/19/99
Tox-120, *BL04199-Sppb-2, 4/19/99
Tox-120, RBL04199-10ppb-2, 4/19/99
Tox-120, RBL04199-2Sppb-2, 4/19/99
Tox-120, BBL04199-50ppb-2, 4/19/99
Tox-120, BBD04199--100ppb-2, 4/19/99
TOX-120, NU.04199-250ppb-2, 4/19/99
Tox-120, *Bl04199-S00ppb-2, 4/19/99
Tox-120, BBL04199-750ppb-2, 4/19/99
Tox-120, XBL04199-1000ppb-2, 4/19/99
MaOH Blank
Tox-120 23M 62 / D1-.2S
Tox-120 24M G2 / Dl:25
Tox-120 25M G2 / Dl:25
Tox-120 23M G2 / DI:25
Tox-120, RBI.03199-- 2S0ppb-CCl, 4/19/99
H e O S Bl'ank
Tox-120 33M G3 / Cl:25
Tox-120 34M G3 / Dl:25
Tox-120 35M G3 / Dl:25
Tox-120 36M G3 / DI:25
Tox-120 37M G3 / Dl:25
Tox-120 36M G3 / DI:25
Tox-120 39M G3 / DI:25
Tox-120 4OH G3 / DI:25
Tox-120, ABL04199--250ppb-CC3, 4/19/99
HaQH Blank
/Tox-120 27M G2 /Tox-120 29M G2 /Tox-120 SOM G2 /Tox-120 31M G2
DI: 10
DI: 10 Dl:10 D i : 10
Tox-120, XBX*04199-250ppb-CC4, 4/19/99
KaOH Blank
/Tox-120 42M G3 /Tox-120 4SM G3 /Tox-120 45M G3 /Tox-120 4 SM G3 /Tox-120 47M G3
DI: 10 DI: 10 DI: 10 DI: 10 DI: 10
Tox-120, RBL4199-250ppb-CC5, 4/19/99
M.OB Blank
Tox-120, RBL04199-H2OBlk-2, 4/19/99
Tox-120, RBL04199-LBlk-2, 4/19/99
Tox-120, BBZi04199-5ppb-2, 4/19/99
Tox-120, BBL04199-- 10ppb-2, 4/19/99
Tox-120, RBL04199-25ppb-2, 4/19/99
Tox-120,- RBL04199-- 50ppb-2, 4/19/99
Tox-120, KBI*04199-100ppb-2, 4/19/99
Tox-120, RBL04199-230ppb-2, 4/19/99
Tox-120, RBl*04199-500ppb-2, 4/19/99
Tox-120, ABL04199-750ppb-2, 4/19/99
Tox-120, RBD04199-1000ppb-2, 4/19/99
Typ Analyta Analyta
Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
Analyta Analyta Analyta Analyta Analyta
OC Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta
OC Analyta
Analyta Analyta Analyta
Analyta OC Analyta
Analyta Analyta Analyta Analyta Analyta
OC Analyta
Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
Std Cono
5.56 11.10 27.80 55.60 111.00 278.00 * 556.00 833.00 1121.00
278.00
278.00
278.00
278.00
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
XT
3.050
3.047 3.030 3.049 5.049 5.033 3.043 5.048
5.050 5.048 5.049 5.049 5.049
5.048 5.050 5.050 3.050 5.053 5.049 3.048 5.049 5.049
5.050 5.048 5.049 5.050 5.050
5.048 5.049 5.048 5.049 5.048 5.049
5.052 5.048 5.049 5.051 5.050 5.049 5.050 5.050 5.049 5.048 5.051
Paga 1
Asta
Xtsponn
Cena. Flag IDc t
72
132 113 175 406 801 1123 1283
368 592 283 255 466
383 601 481 . 515 503 591 465 598 485
440 268 281 326 508
263 563 214 177 288 471
81 58 113 39 139 105 249 429 837 1175 1380
71.666
132.374 113.329 175.242 406.110 800.930 1122.779 1282.724
368.371 592.299 282.603 254.927 463.685
382.606 601.330 480.556 515.341 502.581 590.622 464.784 597.567 485.149
439.886 268.097 280.775 325.677 508.135
262.546 562.997 213.511 177.013 287.691 471.073
81.083 57.712 112.987 38.519 138.857 104.892 248.672 429.161 837.128 1175.497 1379.791
9.88 Mf
78
53.49 bb 41.18 bb
87.71 bb 231.22 bb 557.95 bb
799.83 bb 920.04 bbX
100
-26 -21
-6 0
-4 -17
232.86 bb 401.15 bb 168.40 bb 147.60 bb 304.49 bb
\\
10
243.55 bb 407.94 bb 317.17 bb 343.31 bb 333.72 bb 399.89 bb 305.32 bb 405.11 bb 320.62 bb
266.60 bb 157.50 bb 167.02 bb 200.77 bb 337.90 bb
// \1S ( 22
153.32 bb 379.13 bb 116.47 bb
89.04 bb 172.22 MK 310.04 bb
12j'/j
16.95 KH
0.00 MH
40.92 fabX 636
0.00 MK
-100
60.37 bb
117
-i34.84 bb -37
142.90 bb
. 29
278.54 bb
0
585.15 bb
5
839.45 bb
1
992.99 MMX
-11
BESTCOPYAVAILABLE
S o u p 0 2 0 1 9 9 , K o v lo v o d odi q u a n t i t a t a r i toy d r b 5 / 1 7 / 9 9
000662
TOX-120 Rat U v a s Dilution 1080 1367 1207 1023 MHZ bbl MMI MHZ MHZ
Soup 020199, R f t v i m d and quantltatad by drb 5/17/99
& &
H MH H 3222
S X mXXXXX
HHHH
2222
Is r
pk s I . Ss s SSssp
iiii
3SsS ssc ssa ss
S2aS m
S isa s
8SS53
32223
S3SS S3S535SS I l i a S5SSS
sasssssss 3 "388=5353
3
s
s
s5
s sassgggg
i S B gjgg
31111*3 5 5 . . i P P P i P * * * *
m
m
m
m u min n
m u m ih in
/t
n i
ln a n Uljn
i
HtHrmH Hh
PP
* g* e 22
-1
s a
Mi
n ils
assaf aaaaaaaaf saass aaaaa
SaSaSaSjj; NaNsWNSaNaNidg SaSaNaaS? aaaaa
I S888S 833388383 88883 38833 .saaaaaaaass^a^asggisa^sgsa^sssg^asaaagssass^
*3^s<SSaS^S^^SSS^^ m m m nm m m m m m m m m m m m um
iiliiilliliiiliiiliiililiilliilillilllliilliliiiiiilii
JsSSSoSSo33S8oSoooSo88S2SS8SSSooooSSoSoSSoo383S3SSoSsio "-""-"''-''aaaasaaasaaaaaaaaRaaaasssssRsassiisssscsssaBSs
CO
W
o o
1
Quantify Canpound S w a r y Raport TOX-120 Rat Livar Dilution
Paga 3
1 Mano 1 051499061 2 051499062 3 051499063
4 051499064 5 051499065 6 051499066 7 051499067 S 051499068
9 051499069 10 051499070 11 051499071
12 051499072
13 051499073
14 051499074 15 051499075 14 051499076 17 051499077 18 051499078 19 051499079 20 051499080 21 051499091 22 051499082 23 051499083 24 051499084 25 051499085 26 051499086 27 051499087
28 051499088 29 051499089 30 051499090
31 051499091
32 051499092 33 051499093
34 051499094 35 051499095 36 051499096 37 051499097
38 051499098 39 051499099 40 OS1499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 4B 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114
8aspilit Taxt HaOH Blank
HaOH Blank Tox-120, RBL04199-H20BIk-2, 4/13/99
TOX-120, RBW)4199-lBlk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99
Tox-120, BBl*04199-10ppb-2, 4/19/99 Tox-120, KBL04199-25ppb-2, 4/19/99 Tox-120, BBL04199-S0ppb-2, 4/19/99 Tox-120, X8L04199-100ppb-2, 4/19/99 Tox-120, RBL04199-2S0ppb-2, 4/19/99 Tox-120, 9BL04199-500ppb-2, 4/19/9.9 Tox-120, BBX04199-750ppb-2, 4/19/99 TOX-120, RBX041S9-10Q0ppto-2, 4/19/99
HaOH Blank TOX-120 23M 62 / 91:25 Tox-120 24M G2 / DI:25
Tox-120 2SH G2 / DI:25 Tox-120 2 62 / 91:23 Tox-120, RBL04199-250ppb-CCl, 4/19/99
HaOH Blank Tox-120 33H 63 / 91:25 Tox-120 34M 63 / 91:25 Tox-120 3SM 63 / 91:25 Tox-120 36M 63 / 91:25 TOX-120 3TH 63 / 91:25 Tox-120 3SM 63 / 91:25
Tox-120 39M 63 / 91:25 Tox-120 40N 63 / 91:25 Tox-120, BBL04199-250ppb-CC3, 4/19/99
MaOH Blank Tox-120 27H 62 / 91:10 Tox-120 29H 62 / 91:10 Tox-120 30M 62 / 91:10 Tox-120 31M 62 / 91:10 Tox-120, BBI>04199-250ppb-CC4, 4/19/99
HaOH Blank Tox-120 42H 63 / 91:10 Tox-120 43M 63 / 91:10 Tox-120 45M 63 / 91:10 Tox-120 4SM 63 / 91:10
Tox-120 47H 63 / 91:10 Tox-120, RBL04199-250ppb-CC5, 4/19/99
HaOH Blank Tox-120, RBL04199-H20Blk-2, 4/19/99 Tox-120, RBL04199--Ilk-2, 4/19/99 Tox-120, RBL04199-- 5ppb-2, 4/19/99 Tox-120, RBL04199-10ppb-2, 4/19/99
Tox-120, RBL04199-25ppb-2, 4/19/99 Tox-120.- BBL04199-50ppb-2, 4/19/99 Tox-120, BBL04199-100ppb-2, 4/19/99 Tox-120, ABL04199-250ppb-2, 4/19/99 Tox-120, BBL04199-500ppb-2, 4/19/99 TOX-120, RBL04199-750ppb-2, 4/19/99 Tox-120, BBL04199-1000ppb-2, 4/19/99
Typ Analyta Analyta Analyta
Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta OC Analyta Analyta Analyta Analyta Analyta Analyta
Analyta Analyta Analyta
OC Analyta Analyta Analyta Analyta Analyta
QC Analyta
Analyta Analyta Analyta
Analyta Analyta
QC Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
ltd Cono
Kt
5.56 11.10 27.80 55.60 111.00 278.00 ' 556.00 833.00 1111.00
5.466 5.463 5.464 5.464 5.465 5.500
5.464 5.514
278.00
5.515 ' 5.513
5.514
5.514 5.515
278.00
5.514 5.515 5.516 5.516 5.523 5.515 5.514
5.515 5.514
278.00
5.514 5.514 5.514
5.515 5.515
278.00
5.514 5.515 5.512
5.514 5.514 5.515
5.56 11.10
27.80 55.60 111.00
278.00 556.00 833.00 1111.00
5.516
5.515 5.513 5.514 5.516 5.515
5.513 5.516
iru
RftSpOM*
Cono. Flags %Dv
13 34 49 128 253 467 763 764
79 103
59 86 245
90 76 66 69 71 49 . 66 64 241
136 173 116 138 275
111 121
68 93 71 244
13.419
34.422 .48.961
127.672 253.440 466.900
763.341 764.046
10.23 MK
33.18 MK 49.07 MK 135.10 bb
272.54 bb 505.83 bb 829.80 bb
830.57 bbX
78.528 103.445
58.790 86.441 244.847
81.39 MK 108.62 bb
59.82 MK 90.03 bb 263.15 bb
89.556
93.44 bb
76.379
79.04 MK
65.982
67.68 MK
69.198 . 71.19 MK
70.905
73.06 MK
48.849
48.95 MK
66.234
67.95 MK
64.294
65.83 MK
241.294 259.27 bb
135.719 173.108 116.489
137.808 274.745
143.89 bb 184.75 bb 122.87 bb 146.17 bb
29S.83 bb
110.694 121.136
67.748
93.064 71.230
244.465
116.54 bb
127.95 bb 69.61 MM 97.27 bb
73.41 MK 262.74 bb
8 19 -12 22 -2 -9
0 -25
\
-5
-7
6
-5
15
14.743
11.68 MK
5
23
23.309
21.04 MK
-24
50
50.399
50.65 MK *
-9
116 116.114 122.46 b b . io
262 262.095 282.00 bb
1
547 546.872 593.23 bb
7
762 761.863 828.19 bb -1
773
772.968 840.32 MMX
-24
i
1k l j
f
i
j'
S o u p 0 2 0 1 9 9 , R o v io w o d a n d q u a n t i t a t a d toy d r b 5 / 1 7 /9 9
000664
Quantify Ccxnpound Sunnary ltapert TOX-120 Rat U n r DUutiona
45.418 70.127 44.525 47.121 Soup 020199, Sviawd and quantitatad hy drb 5/17/99
SU
NA
CO
! a
i
i
t
sii
. Ssi 558 523
ssis
3835
fi
SSSSggggg
SEE in
! " 5583S553
5
EBBE
g8
55
g SSSSggggg
5 " ^ S r'SSH
i ni m i i n u m i i m i m i n i l .
I ssssi sssss
5 s s a s s s s s * aaaai a a a a a
a I 55355555555 88885 888888885 83883 888883 5555555552
m0 H
1 ^SSSSSSSS^SS^SSSSS^SSSSSSSSS^SSSSS^SSSSaS^SSSSSSS'SSSS
I
iiiiiiiiiiiilliliiiliilifiiiiiiillliiliiiliil
1 lilffl3 H 3 3 m iltii H m m m iH n S U S n im m
2 "SSSSSnSSSSSSSSSSSRSSSriSRSSSSiSgSSSSSSgSgSSSKSS
1/3
<3 00 O O
O
am
rm^ fiHi niitiomi
m
&
3
m aaa
a
g
XPSPSSS
s
I
KKSPSSS
i
s
!
555555
i
!
8853
S
*2
NNrntiArtnHinlfrl|OH
Nw
s
int5n
5
tn
5
5m5
w
'
3 "
o1 x s s s s s s g g 3 "28323
S '5
1fH
0
iH
aa
O5 o r-8 Nr* 3
1n0
r
?
o
Hr t 2
Hn toft
IfOi S13t 7 - 3 7 S1
2 ssisssl min 2S SS S SS frnit 2 3 3 S 5 N*C m m mfiH< 5 3 5 3 Mn 0HWfiiNArn<srn|mH
0i
MS> tn
5in
oo s mr- 5
Mem S S S 2 S S 3 tft inin ininwiin *oo S 5S S S S S S S ran* " * 2 5 3 2 3
555..
Siili
I l i1 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 . i*iiiiiiiiiiiiiiiiiiiiiii
il
mi iiiiti55551 555.555.55s 55.5.51
8 **NNN7
3 3 8 8 ? 8 3 8 3 3 3 8 3 ? 3 3 3 3 ? 8 3 8 3 3 3MiO*-*r4nMoiior`f^
sN* N* v * 0 S S S X S f l
S 83883838888 88833 383338888 88888 88833
8 S i l l l l 3 I I 3 l a 1 s s ! s 3 S 5 a 5 S | S | s 1 s * s a 5 a s a 5 f l 5 8 l a * s . a ,
jM 8 S $ $ S $ $ '3 S S S ! 3 S 3 S 5 S 3 8 S s S 5 5 3 a 3333333'aS '5'5'$$S S W
lllllee83383s8l38
iililiiiliiiiliiiiiilliliiiiiiiilliiiiiilfiliiliililii
Jssssssssssssssssssssssssssssosssssssssssssssssssssssss
CO CO CO
o
o
Soup 020199 Roviowod and quantitatod by drb 5/17/99
Q u a n tif y Ccs p o u n d Suinit r y R t p o r t TOX-120 S a t L i v a r D i l u ti o n s
Sample List: S:\IACT-1.FR0\8a^leDB\O51499o
Last modified: Mon May 17 14:14:20 1909
Method:
S:\ISCT-l.PR0\Math0B\051499o
Last modified: Mon May 17 14:22:39 1999
Job Coda:
Printed:
Tua May 1> 08:22:40 1999
Compound (: IS
# Mima 1 051499061 2 051499062 3 051499063
4 051499064 5 051499065 051499066 7 051499067
0 051499068
9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 18 051499078
19 051499079 20 051499080
21 051499081
22 051499082
23 051499083
24 051499084 25 051499085
26 051499086 27 051499087 28 051499088
29 051499089 30 051499090
31 051499091
32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101
42 051499102 43 051499103
44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114
SanvXaXi.t l u t
M.0H Blank MaOH Blank Tox-120, RBL04199-H20Blk-2f 4/19/99
Tox-120, M104199-LBlk-2, 4/19/99
Tox-120, RBL04199-Sppb-2, 4/19/99 Tox-120, BBL04199-10ppb-2, 4/19/99 Tox-120, RBL04199-2Sppb-2, 4/19/99 Tox-120, RBL04199-50ppb-2, 4/19/99 Tox-120, BBL04199-100ppb-2, 4/19/99 TOX-120, BBL04199-250ppb-2, 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99
Tox-120, 8BLQ4199--750ppb-2, 4/19/99 Tox-120, BBL04199-1000ppb-2, 4/19/99 MaOB Blank Tox-120 23K 62 / Dl:25 Tox-120 24K 62 / Dl:25
Tox-120 234 62 / 01:25 Tox-120 26M 62 / Dl:25
Tox-120, RBL04199--250ppl>-CCl, 4/19/99 HeOH Blank Tox-120 33M 63 / D1:2S Tox-120 34M 63 / 01:23 Tox-120 334 63 / 01:25 Tox-120 304 63 / 01:25 Tox-120 37M 63 / 01:25 Tox-120 3BM 63 / 01:25 Tox-120 39M 63 / 01:25 Tox-120 40M 63 / 01:25 Tox-120, RBL04199-250ppb-CC3, 4/19/99 MeOH Blank Tox-120 27K 62 / 01:10'
Tox-120 29K 62 / 01:10 Tox-120 SOM 62 / Dl:10 Tox-120 31M 62 / 01:10 Tox-120, RBL04199-2S0ppb-CC4, 4/19/99 MaOH Blank Tox-120 42M 63 / Dl:10 Tox-120 43M 63 / Dl:10 Tox-120 45M 63 / 01:10 Tox-120 44M 63 / 01:10 Tox-120 47M 63 / 01:10 Tox-120, RBL04199-250ppfa-CC5, 4/19/99 MaOH Blank Tox-120, BBL04199-H20Blk-2, 4/19/99 Tox-120, BBL04199-lBlk-2, 4/19/99 Tox-120, 9BL04199--Sppb-- 2, 4/19/99
Tox-120, BBL04199-10ppb-2, 4/19/99 Tox-120, BBL04199-25ppb-2, 4/19/99 Tox-120, RB104199--50ppb-2, 4/19/99 Tox-120, RB1.04199--lOOppb-2, 4/19/99
Tox-120, BBL04199--250ppb-2, 4/19/99 Tox-120, BBl04199-500ppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 Tox-120, KB104199-1000ppb-2, 4/19/99
Typ
Analyta Analyta Analyta Analyta Standard
Standard Standard Standard ' Standard Standard Standard Standard Standard
Analyta Analyta Analyta Analyta Analyta
QC Analyta Analyta
Analyta Analyta Analyta Analyta Analyta Analyta Analyta
QC Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyte Analyta
Analyte QC Analyta Analyta Analyta Standard
Standard Standard Standard Standard Standard Standard Standard Standard
Std Cono
St
1.00 1.00 1 a 00 1.00 1.00 1.00 1.00 1.00 1.00
1.00
1.00
1.00
1.00
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
5.036 5.035 5.036 5.036 5.033 5.036 5.034 5.035 5.023 5.034 5.034
5.036 5.034 ' 5.035 5.035 5.035
5.034 3.036 5.036 5.036 5.042 5.035 5.034 5.035 5.035
5.037 5.034 5.035 5.036 5.036
5.034 5.035 5.034 5.035 5.034 5.035
5.038 5.034 5.035 5.037 5.036 5.035 5.036 5.036 5.035 5.034 5.037
Area
2407 2496 2699 2722 2446 2280 2566 2352 2408 2309 2302
81 109
74 70 2336
170 81 71 95 84 83
. 97 73
2253
163 198 184 179 2464
214 175 164
no
161 2414
2416 2396 2542 2751 2SOO 2337 2586 2464 2333 2355 2457
Reponce
Cene. Slags %Dev
2406.723 2496.454 2699.373 2722.382 2446.267 2280.384 2565.513 2351.564 2408.171 2308.686 2301.694
81.237 109.041
74.247 69.750 2336.227
169.517 80.550 71.387 94.601 84.019 82.882 96.582 72.744
2253.416
163.472 198.362 183.888 178.796 2464.372
213.740 174.571 163.908 169.648 160.737 2413.697
2415.683 2395.952 2541.736 2751.209 2500.397' 2336.569 2585.568 2463.607 2333.406 2355.073 2457.479
0.98 bb 1.01 bb
1.09 bb 1.10 bb
0.99 bb 0.92 bb 1.04 bb 0.95 bb 0.98 bb 0.94 bb 0.93 bb
-2
1 9 10 -1 -8 4 -5 -2 -6 -7
0.03 bb 0.04 bb 0.03 m
0.03 MM 0.95 bb
-97
-96 -97 -97
-5
0.07 bb 0.03 bb 0.03 MM
0.04 bb 0.03 bb 0.03 bb 0.04 bb 0.03 MM 0.91 bb
-93 -97
-97 -96 -97 -97 -96 -97
-9
0.07 bb 0.08 bb 0.07 bb 0.07 bb 1.00 bb
-93
-92 -93 -93
0
0.09 bb 0.07 bb 0.07 bb 0.07 bb 0.07 bb 0.98 bb
-91 -93 -93 -93 -93
-2
0.98 bb 0.97 bb 1.03 bb
1.12 bb 1.01 bb 0.95 bb * 1.05 bb 1.00 bb : 0.95 bb 0.95 bb 1.00 MM
-2 -3
3 12
1
-5 5 0
-5 -5
0
Paga 6
Soup 020199, S a v ia wad an d q u a n tita te d b y d sb 5 /1 7 /9 9
000667
Q u an tify C a lib ra tio n S a p o rt
Tax-120 a t l i v a r D i l u t i o n s
Calibration s B:\raCT-l.PRO\CurvarB\05UaJa
Last aedifiad: Tua May 1 01:21:03 1999
Printad:
Tua May 18 00:22:33 1909
Paga 1 *
Ooup 020199, K aviavad and q u a n tita ta d b y dxb 3 /1 7 /9 9
000668
Q u an tify C a lib ra tio n R ap o rt TCX-120 b a t Zalvor O l l u tl o n a
Calibration: i:\rACT-l. rao\CurvDB\051499o
Laat aodlflad: Tun May 18 08:21:03 1999
Prlntad:
Tua Kay 18 08:22:83 189
Paga 2
000669
Q u an tify C a lib ra tio n P a p o rt T0K -120 P a t L lv a r B llu tlo n a
C a lib r a tio n s S :\yP C T -l.P 9O \C urvaD B \0S 1499a
l a a t n o d l f i a d i T ua May 1* 0 3 :3 1 :0 3 1339
P rln ta d :
T ua May 13 0 3 :2 3 :3 3 1999
Pago 3
Q u a n tify C a lib ra tio n R ap o rt TOX-120 R a t U v a r D llu tio n a
Calibration: I:\Plcr-l.PRO\CurMD8\051499o
Laat nodlflad: Tun May It 08:21:03 1989
trlntad:
tun Kay 19 08:22:53 1999
Paga 4
Soup 0 2 0 1 9 9 , R arvlaw ad a n d q u a n t i t a t a d b y d r b 5 /1 7 /9 9
000671
Q u an tify C a lib ra tio n K aport TGX-120 M at L lv a r O il u tlo n a
C a l i b r a t i o n 8 :\F A C T -1 .PR O \C urvoD B \Q 51498c
L a s t a o d if l a d : Tua M ay 18 0 8 :2 1 1 0 3 1888
P rin ta d :
Tua May 18 0 8 :2 3 :3 3 1888
faga 5
Soup 020188, Raviawad and quanti tatad by drb S/17/88
000672
1
Q u a n t i f y Ca l i b r a t i o n B a p o r t
(ax-120 B a t U v a r D l l a t l a n a
lalibration! S:\XBCT-l.Pie\eanrtfB\0S14BBa
1 U a t aodiflad: Tua May IB 08:21:03 1898
(rintad:
Tua May IB 08:22:53 1999
Compound 6 name: IS
1
Response Factor. 2467.17 Response type: External Std, Area
Curve type: RF
I
1
3
I
1
1 !
;
i
I
I
J
1
3
!
J
;i
?
Paga
Method Report
Method file: Last modified:
Compound:
Printed:
S:\IACT-l.PBO\MethDB\051499c.mdb Mon Hay 17 14:23:3 19990
1: PQAA
Tue Hey 18 09:41:43 1999
Compound Name: Zntemel Ref.: Quantify Trace: Cone, of Standards:
User fUT Value: User Peak rector: Integrate Window rector:
Peak location
Retention Time (mins) : Relative Retention* Time: Time Window (mins) :
Peak Selection:
Response
Response Type: Response:
Calibration Curves
Polynomial Type: Point of Origin: rit Weighting: Axis Transformation:
Concentration
Units:
Noise
Peak-to-peak amplitude:
Smooth
Smoothing: Window sire (scans): Number of smooths: Smoothing method:
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
Peak Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold:
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
POAA [None] TZC Cone. A 1.000000, Disabled 1.000000 2 .0 0 0
4.998, Enabled 0.000, Disabled 0.400 Nearest
External Areas
Linear Exclude 1/X None
140
Disabled 3 2
20.00 100.00 0.00
10.00 Disabled 40.00
1.50, Disabled 0.00, Disabled 2.00, Disabled 90.00, Enabled
Page l
000674
M athod R e p o rt
Mathod fila: Last modified:
Compound:
P rin te d :
9:\FACT-l.PRO\MethDB\05I499c.mdb Mon May 1? 14:23:39 19990
2: M463
Tua May 18 0 9 :4 1 :4 3 1999
Conpound Hama: Internal Ref.: Quantify Traeai Cone, of Standards:
Caer RT Valua: Oaar Peak ractor: Intgrata Hlndow factor:
Peak Location
Retention Time (mins): Relative Retention Time: Time Window (mins):
Peak Selection:
Response
Response Type: Raeponse:
Calibration Curves
Polynomial Type: Point of Origin: Pit Weighting: Axis Transformation:
Concentration
Dnita:
Noise
Peak-to-pak anpiitndei
Smooth
Smoothing: Window size (scans): Number of smooths: Smoothing method:
Bsselines
Join valley threahold: Rduca peak tailing: Raiaa baseline naxlxun:
Peak Seoaration
Draw vertical threahold: Detect ahouldera: Shoulder threahold:
Response Threshold
Relative height: Rbaolute height: Relative area: Absolute area:
M463 [None] TIC Cone* A 1.000000, Disabled 0.000000 2.000
5.40, Enabled 0.000, Disabled 0.551 Nearest
external Areas
Linear exclude 1/X Hone
140
Disabled 3 2
20.00 100.00 0.00
10.00 Disabled 40.00
1.50, Disabled 0.00, Disabled 2.00, Disabled 90.00, Enabled
Sp/in 0 2 0 1 ^ --------------------------------------------
W c ojt/ W m
.X
Paga 2
000675
Hathod Report
Method file: Last Modified:
Compound:
Printed:
S:\PACT-l.PAO\MethBB\OS1499e.mdb Mon May 17 14:23:3 19990
3: MS13
Tue May 18 01:41:45 1999
Compound Hama: Internal Ref.: Quantify Trace: Cone, of Standards:
Uses RF Value: D i m Peak Factors Integrate Window Factors
Paak Location
Retention Time (mine): Relative Retention Timet Tina Window (mins):
Paak Selection:
Response
Raspons. Type: Response:
Calibration Curves
Polynomial Typos Point of Origin: Fit Weighting: Axis Transformation:
Concentration
Units:
Holao
Pealc-to-pealt aapiltude:
Smooth
Smoothing: Window size (scans): Humber of smooths: Smoothing method:
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
Paak Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold:
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
M513 (Hone) TIC Cone. A 1.000000, Disabled 1.000000 2 .0 0 0
5.538, Knablad 0.000, Diaablsd 0.325 Maaraat
external Areas '
Linear Exclude 1/X None
140
Disabled 3 2
20.00* 100.00 0.00
10.00 Disabled 40.00
1.50, Disabled , 0.00, Disabled
2.00, Disabled 80.00, Enabled
Sp(r*ya fr lP ill
Page 3
000676
M ethod R e p o rt
Method file: test Bodifled:
Compound:
P rin te d :
S:\IACT-l.PRO\Nathna\0S1499c.ndb Mon Key 17 14:23:3 19990
4: M S 63
Tue Key 1 0 8 :4 1 :4 1999
Compound Nana: Internet Ref.: Quantify Trees: Cone, of Standards:
User RF Vslusi Usar Peak Factors Integrate Window Factors
Paak location
Ratantion Time (Bins) : Relative Ratantion Tina: Tina Window (mins)s
Paak Salactions
Raaponaa
Rasponsa Type: Rasponsas -
Calibration Curves
Polynomial Typas Point of Origins Fit Weighting: Axis Transformations
Concantratlon
Units:
Koisa
Peak-to-paak amplitude:
Smooth
Smoothings WlndoH size (seens): Mumbar of smooths t Smoothing methods
Basallnas
Join valley threshold: Reduce peek telling: Relse baseline Milieu:
Peek Ssuaretlon
Drew vertical threshold: D a t e d shoulderss Shoulder threshold:
Response Threshold
Rslstive height: Absolute height: Relative area: Absolute area:
MSC3 (None] TIC Cone. A
1.000000, Dlaablad 1.000000 2 .0 0 0
5.349, Enabled 0.000, Dlaablad 0.51B nearest
External Areas
Linear Exclude 1/X Mona
140
Disabled 3 2
20.00 100.00 0.00
10.00 Dlaablad 40.00
1.50, Disabled < 0.00, Disabled
2.00, Disabled 10.00, enabled
'tH P n 0 liefr\
tx ito lfl
Paga 4
|
000677
Method Report
Method fllo: Loot modified:
Com pound:
P rin te d :
S:\FACT-l.PR0\MethDB\0S1499c.mdb Mon Hoy XT 14:23:3 19990
5 : H613
Tue May 18 0 1 :4 1 :4 6 1999
Compound Nemo: Internal R e f .: Quantify Trace: Cone, of Standards:
Deer RP Value: Dser Peak factor: Integrate Window factor:
Peak location
Retention Time (mins): Relative Retention Time: Time Window (mins):
Peak Selection:
Response
Response Type: Response:
Calibration Curves
Polynomial Type: Point of Origin: fit Weighting: Axis Transformation:
Concentration
Chits:
Mol am
Peak-to-pest amplitude:
Smooth
Smoothing: Window sis (scans): Number of smooths: Smoothing method:
Baselines
Join valley threshold: Reduce peak tailing: Raisa baseline maximum:
Peak Seoaration
Draw vertical threshold: Detect shoulders: Shoulder threshold:
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
M613 [MOne] TIC Cone. A 1.000000, Disabled 1.000000 2 .0 0 0
5.S3, Enabled 0.000, Disabled 0.27S nearest
External Areas
lilnesr Exclude 1/X Hone '
140
Disabled 3 2
20.00 100.00 0.00
10.00 Disabled 40.00
1.S0, Disabled , 0.00, Disabled
2.00, Disabled 0.00, Enabled
Peg* S
000678
Method Report
Method file: last Modified:
Conpound:
Printed:
S:\lKT-l.PRO\MethDB\051499c.ndb Mon M ay 17 14:23:38 19990
6: is
Tue May 18 08:41:48 1999
Conpound Nana: Internal Ref.: Quantify Trace: Cone, of Standards:
Oser RT Value: Daer Peak Factor: Integrate Window Factor:
IS [Done] TIC fixed
1.000000, Disabled 1.000000 2 .0 0 0
Peak Location
Retention Tine Inins): Relative Retention Tine: Tine Window Inins):
5.034, Enabled 0.000, Disabled 0.200
Peak Selection:
Nearest
Rasoonsa
Response Type: Response:
external Areas
Calibration Curves
Polynomial Type: Point of Origin: Fit Weighting:
Axis Transformation:
Linear Exclude
1 /X Hone
Concentration
Units:
Holsa
Peak-to-peak amplitude:
140
Smooth
Smoothing: Window size (scans}:
Humber of smooths: Smoothing method:
Disabled
3 2
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline nsxiaun:
20.00 100.00 0.00
Peak Sesaratlon
Draw vertical threshold:
Detect shoulders:
Shoulder threshold:
0
10.00 Disabled 40.00
Response Threshold
Relative height: Absolute height: Relative area:
Absolute area:
1.50, Disabled t 0.00, Disabled
2.00, Disabled *0.00,. Enabled
b D L 1!
M c, x /i /^9
rage 5
000679
SOUP 020199: FACT Equipm ent L og 99014
Q uattro II, #6414E
DATE: 05/21/99
ANALYST: drb
STUDY NUMBER: TQX-120 MATRIX:Rat Liver _DESCRIPTION: Pilutjons/Tclmer FACT Method: ETS-8-$Q ^
Injection volume: 10 uL
Cycle time:.. ? TOIL Electrode:.Z-$pi ay-----------
M obile Phase:
fAtt.OmM NH4CH3CQ2
(B).MsOH--------------- :--
TN-A- or stndrd number (A) '99011-6-9_________ .
(B )T-A-254S
Prepared (date/by): Expires:
(Al 04/28/99 drb_______ _ (A) 10/99----------------------
(B) 05/^1/99 a h fBl 10/99
Store at room temp? @ or N
Other:.
Guard column: None__________________
Part number: na_________ ' Lot number:jja--------------------------------
A nalytical colum n: Keystone Bctasil
-------------------------------- _ _ _ _ _
Size: 2 x SO mm________________ Type: C l8____________ Sphere size:Jjm a----------
Part number: 055-701-2__________ Lot number: P7LQ4 Serial number:Q481175Z
A nalytical parameters:
Drying gas (IVhrl: 350
ESI nebulizing gas (L/hr)J5___ Sheath gas (L/hr)_0
LC Flow rate: 300 uL/min, Back pressure: 55 bar
HPLC solvent gradient: 9min-2
Tinte (m in.l 110.00 211.00
3 1 4 ,5 0 4 1 6 .5 0 51 7.00
-%B 40,0 40.0_ _95.Q_ 95.0 40.0_
000680
Tuning Method Report
Method:
C :\MASSLW0C\PACT-1 .PR0\ACQTOB\PF0S2
Printed:
Fri May 21 16:28:16 1999
Rage 1
best copy available
MS
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
MS2
Set Rdbk
3.01 40
5 0.2 150 250
3.01 -46 -8
153 254
Pressures
Analyser Vacuum Gas Cell
Rdbk
2.4e-4 4.8e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
."x
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
14.0 14.0 2.0 -Jl o.o ''* ' 53 650
Set Rdbk
i5.0 15.0 45 43 2.0 0.0 40 39 0 43 650
000681
BESTCOPYAVAILABLE
M aaslynx - Sam ple List
Sample List: C:\MASSLYNX\FACT-1 .PRO\Sam pleDB\052199b.SPL Printed: Fri May 21 16:28:031999
Fite Nam e
Fite T ext
1 052199068
MeOH Blank
2 052199069
MeOH Blank
3 052199070
Tox-120, R 8L04199-H 20B lk-1,4 /1 9 /9 9
4 052199071
Tox-120, RBL04199-LBIk-1,4/19/99
5 052199072
Tox-120, RBL04199-5ppb-1,4/19/99
6 052199073
Tox-120, RBL04199-1 Oppb-1,4/19/99
7 052199074
Tox-120, RBL04199-25ppb-1,4/19/99
8 052199075
Tox-120, RBL04199-50ppb-1,4 /1 9 /9 9
9 052199076
Tox-120, RBL04199-100ppb-1,4 /1 9 /9 9
10 052199077
Tox-120, RBL04199-250ppb-1,4 /1 9 /9 9
11 052199078
Tox-120, RBL04199-500ppb-1,4 /1 9 /9 9
12 052199079
Tox-120, RBL04199-750ppb-1,4 /1 9 /9 9
13 052199080
Tox-120, RBL04199-1000ppb-1,4 /1 9 /9 9
14 052199081
MeOH Blank
15 052199082
Tox-120 4 9 M G 4 /D 1 :1 0
16 052199083
Tox-120 5 0 M G 4 /D 1 :1 0
17 052199084
Tox-12051M G 4 /D 1 :1 0
18 052199085
Tox-120 5 2 M G 4 /D 1 :1 0
19 052199086
Tox-120, RBL04199-250ppb-CC1,4 /1 9 /9 9
20 052199087
MeOH Blank
21 052199088
Tox-120 5 3 M G 4 /D 1 :1 0
22 052199089
Tox-120 5 4 M G 4 /D 1 :1 0
23 052199090
Tox-120 5 5 M G 4 /D 1 :1 0
24 052199091
Tox-120 5 6 M G 4 /D 1 :1 0
25 052199092
Tox-120. R BL04199-250ppb-CC3,4/19/99
26 052199093
MeOH Blank
27 052199094
Tox-120 6 5 M G 5 /D 1 :5 0 0
28 052199095
Tox-120 6 6 M G 5 /D 1 :5 0 0
29 052199096
Tox-120 6 7 M G 5 /D 1 :5 0 0
30 052199097
Tox-120 6 8 M G 5 /D 1 :5 0 0
31 052199098
Tox-120, RBL04199-250ppb-CC11,4/19/99
32 052199099
MeOH Blank
33 052199100 Tox-120 69M G 5 /D 1 :5 0 0
34 052199101
Tox-120 70M G5 / 0 1 :500
35 052199102
Tox-120 71M G 5 /01:500
36 052199103
Tox-120 7 2 M G 5 /D 1 :5 0 0
37 052199104
Tox-120, RBL04199-250ppb-CC1,4 /1 9 /9 9
38 052199105
MeOH Blank
39 052199106
Tox-120 7 3 M G 5 /D 1 :2 5 0
40 052199107
Tox-120 7 4 M G 5 / D1:250
41 052199108
Tox-120 7 5 M G 5 /D 1 :2 5 0
42 052199109
Tox-120, R BL04199-250ppb-CC11,4/19/99
43 052199110
MeOH Blank
44 052199111
Tox-120 7 7 M G 5 /D 1 5 5 0
45 052199112
Tox-120 78M G5 / D1:250
46 052199113
Tox-120 79M G 5 /01:250
47 052199114
Tox-120, RBL04199-250ppb-CC1,4 /1 9 /9 9
48 052199115
M eO H Blank
49 052199116
Tox-120, R BL04199-H20Blk-1,4 /1 9 /9 9
50 052199117
Tox-120, R BL04199-LBIk-1,4/19/99
51 052199118
Tox-120, RBL04199-5ppb-1,4 /1 9 /9 9
52 052199119
Tox-120, RBL04199-1 Oppb-1,4/19/99
53 052199120
Tox-120, RBL04199-25ppb-1,4 /1 9 /9 9
54 052199121
Tox-120, RBL04199-50ppb-1,4/19/99
55 052199122
Tox-120, RBL04199-100ppb-1, 4/19/99
56 052199123
Tox-120, RBL04199-250ppb-1,4 /1 9 /9 9
57 052199124
Tox-120, RBL04199-5Q0ppb-1,4 /1 9 /9 9
58 052199125
Tox-120, RBL04199-750ppb-1, 4/19/99
59 052199126
Tox-120, RBLQ4199-1000ppb-1, 4/19/99
M S File
TEU -PF2 TE -PF2 T E L I-P F 2 T E L I-P F 2 TEU -PF2 T E L I-P F 2 TEU -PF2 T E L I-P F 2 TEU -PF2 . TEU -PF2 T E L I-P F 2 TEU -PF2 TEU -PF2 TE -PF2 TEU -PF2 TEU -PF2 TEU -PF2 T E L I-P F 2 TE -PF2 T E -P F 2 TEU -PF2 TEU -PF2 TEU -PF2 TEU -PF2 T E -P F 2 T E U -P F 2 TEU -PF2 T E U -P F 2 TEU -PF2 T E L I-P F 2 T E L I-P F 2 T E L I-P F 2 TEU -PF2 T E U -P F 2 TEU -PF2 T E U -P F 2 TE -PF2 TEU -PF2 TEU -PF2 TEU -PF2 TEU -PF2 T E U -P F 2 TEU -PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 TEU -PF2 TEU -PF2 TEU -PF2 T E U -P F 2 TEU -PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E L I-P F 2 T E U -P F 2
In let File B ottle ilum e
9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m In-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9m In-2 9m ln-2 9m in-2 9m ln-2 9m in-2
9m ln-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9min-2 9m in-2 9mln-2 9m in-2 9m in-2 9mtn-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m In-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9min-2 9min-2 9mln-2 9m in-2 9m in-2 9m In-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m In-2 9m ln-2 9m ln-2
91
91
79 80 81 82 83 84 85 86 87 88 89 92 57 58 59 60 90 96 61 62 63 64 99 92 65 66 67 68 100 93 r 69 70 71 72 90 94 73 74 75 100 95 76 77 78 90 96
79 80 81 82 83 84 85 86 87 88 89
.
10
10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 to 10 10 10 10 10 10 10 10 10 10
000682
Scanning1Method Report
Method:
C: \KU8tfna\FACT-l.TS0\JU5QOBS\TSLX-TF2
Last Modified: Tri Hay 21 15:13*53 1999
Triated:
Tri May 21 16:20:25 1999
olvwt Mliy ( a i m I t
0.00
Function s 1
MM( of 2 Mesa M i n ( m - )
Xntar O u n l Dilay ( Sees ) : Span ( Dalton. > i
Sturt Tima ( >lias ) i Sad Tina ( Mins ) i Kapaatf i
Channel Taraat Daughter
0.03 0.00 0.00 9.00 1 Dwell (S.c.) Coll Energy (*V)
Con. ( V )
1
399.00 99.00
0.25
2
399.00 130.00
0 *2 5
45 45
60 60
Function i 2
MRM of 2 Mass Taira ( sar- >
Zatar Channel Delay ( Sac. ) Spaa ( Daltoas ) t Start Tim. ( Mia. )
Sad Tine ( Mias ) : Repeats :
Channel Taraat Daughter
0.03
0.00 0.00 9.00
1 Dwell (Seca) Coli B u r g y
(V)
. Con. C V )
1
299.00 99.00
0.25
2
299.00 130.09
0.25
45 43
60 60
Juaetloa t 2
W M of 1 Mass Talr ( U T - )
Intar Channel Daisy ( Secs ) t 0.03
Spaa ( Daltons ) * Start Tina { Mias ) :
0.04 0.00
Sad Tina { Mias ) :
9.00
Repeats :
1
Channel parent Daughter Ovali
(Sacs)
Coll Knargy
<aV)
Cono ( V )
1
427.00 80.00
0.23
40
00
**ge i
000683
FACT: DATA REVIEW SUMMARY FORM
Date:
S M H S _______
000684
Tocnao-cs dilati
a p i* U s ti cxm iiL nm rA C T -i.fK o\*^ia6a\03ais
L u i a o d l t l t d * Mon Mty 24 0 0 i0 3 i0 9 1 H I
M athedi
e I\jttj* L Y \w u rr-i .9no\M atfcn\os3i99k
U * t a a d i t l a i i Noa May 24 0 7 iS 9 i4 4 1991
t r i t li
Iter 34 O ltlO iSS 1999
it m a
Nmm 1 052199044
2 052199049 1 052199070 4 052199071
S 052199072 4 052199073
7 052199074 052199075 9 052199074 10 052199077 11 052199079 12 052199079 13 092199040 14 052199041
15 052199042 14 052199043 17 052199044 14 052199045 19 052199044 20 052199047 21 052199044 22 052199049 23 052199090 24 052199091 25 052199092 24 052199093 27 052199094 24 052199095 29 052199094 30 052199097 31 052199094 32 052199099 33 052199100 34 052199101 35 052199102 34 052199103 37 053199104 34 052199105 39 052199104 40 052199107 41 052199104 42 052199109 43 052199110 44 0S21991U 45 052199112 44 052199113
47 052199114 44 052199115 49 052199114 50 052199117
51 052199119 52 052199119 53 053199130 54 052199131 55 052199132 54 052199123 57 052199124 54 052199125 59 052199124
S tx p ltlla t tank MtOH S ita le MtOH S ita le Tox-120. M lL04139-K20Slk-l, 4/19/99
Tox-120, xaL04199-bBlfc-l. 4/19/99 TOX-120, HBL04199-Sppb-1, 4 /19/99 TOX-120, HBL04199-10ppb-l, 4 /1 9 /9 9 Tox-120, XSL04199-25ppb-l, 4/19/99 Tox-120, M L04199-50ppb-l, 4/19/99 T ox-120, ftBL04199-100ppfe~l, 4 /1 9 /9 9 Tox-120, M L04199-250ppb-l, 4/19/99 Tox-120, M U 4199-500tpb-l, 4/19/99 Tox-120. XBL04199-750ppb-l, 4/19/99 Tox-120, XBL0419t-1000ppb-l, 4/29/99 MoOH a ita la Tox-120 49M 04 / O l i 10 Tox-120 SOM 04 / 01*10 Tox-120 S1M 04 / O l i l O Tox-120 52M 04 / 01*10 Tox-120, UL04199-250ppb-CCl. 4/19/99 Mao a ita le Tox-120 S3M 04 / 0 1 :1 0 Tox-120 S4M 04 / O lilO TOX-120 S5K 04 / O lilO
Tox-120 5 SM 04 / 01*10 Tox-120, ML04199-250ppb-CC3, 4/19/99 Mooa a ita le Tox-120 <5M OS / O l i 500 Tox-120 44M OS / O l i 500
Tox-120 47M OS / OliSOO TOX-120 4M OS / 01*500 Tox-120, M U 4199-2S tlprh CC11, 4/19/99 Htoa a ita li Tox-120 C9M 05 / 01*500 Tox-120 70M OS / 01*500 Tox-120 71M OS / 01*500 Tox-120 72M OS / 01*500 Tox-120, RBL04199-250ppb-CCl 4 /1 9 /9 9 MtOH a ita le Tox-120 73K OS / 01*250 Tox-120 74M OS / D lt2S0 Tox-120 7 SM OS / DI 250 TOX-120, M LO4190-25Oppb-CCll, 4/19/99 MtOH a ita le Tox-120 77M 05 / 01*250 Tox-120 7W Q 5 / 01*250 Tox-120 79M OS / 0 1 i2S0 Tox-120, XabO42JJ-250pffe-CCl, 4/29/99 MtOH a ita le Tox-120, JtaL04199-K2Oai)c-l, 4 /1 9 /9 9 Tox-120. K U 04199-LBlk-l, 4/19/99 Tox-120, RBL04199-Sppl>-1, 4 /1 9 /9 9
Tox-120, RSL04199-10ppb-l, 4/19/99 Tox-120, U L04199-29ppb-l, 4/19/99 Tox-120, 2BU)4199-S0ppb-1, 4/19/99 Tox-120, U L 04199-100ppb-l, 4/19/99 T ox-120, MBL04199-2S0ppb-l, 4 /1 9 /9 9 Tox-120, KBL04199-500ppb-l, 4/19/99 Tox-120, aaL04199-750ppb-l, 4/19/99 T ox-120, 2BC,04199-I000ppl>-1, 4 /1 9 /9 9
Typt A atly tt A atly tt A atly tt A atly tt S ta n d ard Standard S ttad trd Standard S ta n d ard Standard Standard Standard S e a n d trd A atly tt A atly tt A atly tt A atly tt A atly tt
OC A atly tt
A atly tt A atly tt A atly tt A atly tt oc A atly tt A atly tt A atly tt A atly tt A atly tt
OC A atly tt A atly tt A atly tt A atly tt A atly tt
OC A atly tt A atly tt A atly tt A atly tt oc A atly tt A atly tt A atly tt
A atly tt OC A atly tt A atly tt A atly tt S ttad trd S ttad trd
S ttad trd S ttad trd S ttad trd S ttad trd S ttad trd S ttad trd S ttadtrd
s td eoa
5.54 11.10 27.00 55.40 111.00 270.00 550.00 033.00 1112.00
270.00
270.00
270.00
270.00
270.00
270.00
5.50 11.10 27.00 50.40 111.00 271.00 554.00 33.00 1111.00
ta
4.040 4.040 4.045 4.545 4.045 4.045 4.049 4.045
4.040
4.040
4.045 4.045 4.045 4.045 4.040
4.045 4.045 4.045 4.045 4.040
4.045 4.045 4.051 4.0SS
4.445 4.145 4.145 4.04S
4.040 4.045 4.045 4.045 4.045 4.045 4.045 4.045
A r t t 81 ,, C ena, y i t a t MDtnr
10 33 77 133 372
720 1173 1315
1 0 .1 5 MI 1 25 .1 3 MI 1 5 7 .1 4 MM 1 99.00 bb
1 274.74 bb 1 530.90 bb 1 043.01 bb
1 9 4 0 .9 4 bbX
-27 -10
4 -11
-1 -4
4 -13
433 1 319.33 bb
15
454
90S 902 775 925 3<1
944 OSO 400 1059 459
379 240 347 324
203 320 151 430
*
1 334.73 bb
1 725.97 bb 1 723.09 bb 1 570.93 bb 1 4 1 1 .7 4 MI 1 244.47 bb
1 497.07 bb 1 420.40 bb 1 442.71 bb 1 740.20 bb 1 330.75 bb
1 279.57 bb 1 192.45 bb 1 270.91 bb 1 341.10 bb
1 150.55 bb 1 140.00 bb 1 111.00 MI 1 323.44 bb
30 -4 32 -13 14
11
41 3 130
391 749 1134 1297
1 1 3 .9 4 MM 1 31.34 m 1 0 1 .9 3 MI 1 1 0 2.30 U 1 200.42 bb
1 552.14 bb 1 035.44 bb 1 955.47 bbX
24
13 11 0 4 -1 0 -14
>i 1 5
-- 0201J* t g u n t l u M b y u l p r i n t d b y k jk U / / l l
000685
Ott 090191t O M B ti U t i kV u p r i n t b y k j k 08/94/91
f W H l
i
5r r
SS
SSKuS g
""""" 5SSUU
9
ilglillisf I 3
Iz k & l i i i l s i l i l l !
................................................................... ?
r.*r.rrrrr." ? ?
o
o
uSSSSSSSSSS 3 3 3? . 3
SSS3S Oooo awsawwwwo
i
O MMV MMM % % V % t f i f S VMM MMV MMM !
o i1
M k
S
iU
tk W U U HUI S.I I
f
0
&
GO
01
>v-
?
Kathod E ilet Last aedU iw lt
Compound!
P rin ted i
Ct \KASSLYNX\PACT*1 .PRO\M ethDR\O2199b.ndb Men Nay 24 0 7 iS 9 s 4 t 11*9
li i m a
Man May 24 0 i l4 t2 1 1*9
In carn ai R ef.i Q uantify Traeat Caos, f Standards
U s a r MT V a lu si Usar H ak F aeto n I n t e g r a t a Window F a e t o n
tMona) 399.00 > 99.00
1.000000, D isaU ad 1.000000 2 .0 0 0
n a ta n tien f in a (mina) R elativ a R etention Tinsi T in e Window (m ina) t
Peak S electio n t
4.040, Raablad 0.000, O issblad 0.599
H aarest
Raapensa Typst Rospensa
B etonai Areas
P elyaesdal Typet P o in t e i O rigini P i t Weight i n g i
Axis Transform ation
L in e ar R x cluda
1/X Mane
U nitsi
Peak-to-peak aapliC udat
--
i n n i i t mi i Window s i s a ( s c e n a ) i JAm b a r o f s n o o th s P ioothing awthedi
114
O issblad 3 2
Jo ln v a lle y threeheld* Reduce peak ta ilin g i R aiaa b aselin a naxiaunt
20.00
100.00 0.00
Draw v e r t i e a l tb ra s b o ld i
O aeact sheuldorsi Shoulder threshold
10.00
O issblad 40.00
R elativa h a l i t i A bsoluta h a lg h ti R elativa arasi A bsoluta a ra si
1.50, O issblad 0.00, O issblad
2.00, O issblad 00.00, feab led
S p u j} o i o i w ____I C m / ^ y / 9 ?
for*
000687
Method R ep art Method f i l e t I n s t m odified
P rin te d
Ci \KA5SLYMX\FACT-l.PIIO\MechDS\032l9b.i Men May 24 07s994* 1999
2t IS
Men May 34 0 0 1 4 t2 1 1999
In te rn a l R ef.s Q uantify Trace Cone, o f Standards
U s e r RT V alue V eer Peak Factors I n t e g r a t e Window F a c to r s
IS (Rene] TIC F in e d
1.900000, D isabled 1.000000 a.ooo
R etention Tine (alas) t R e la tiv e R ten tio n Tinas T in e Window ( n ia e ) s
Peak S electleas
9.130. 0.000, D isabled 0.420
Response Types Reopens# t
I Polynomial types P o in t e f O rigins F i t W eighting Axle T ransform ations
^ units
S xteraal Arena
P eak-to-peek am plitudes
S m oothing Window s i r e ( s e a a s ) i
Hunker e f smooths W snotMng methods
D isabled 3 2
J o in v a lle y threshold Reduce peak t a i l in g > R aise b aselin e naxiaust
Draw v e r t i c a l th re sh o ld s D etect shoulders Shoulder thresholds
2 0 .0 0 100.00 0.00 '
10.00 D isabled 40.00
R elativ e heights A bsolute h eights R elative areas
A bsolute areas
1.90, D isabled 0.00, D isabled 2.0 0 , D isabled
00.00. feebled
J
3
n a o m LAc o s / u f a
fiflt 2
000688
Q uatlfy e llM ttiM to pan T0*130-03 d i l a t i ocui
crtlteftfelM it ci\M M ttnonncr~ i.9jio\cuxvD a\os3i9k
U * t M ditU A t N a a to y M N iN iM U N
PrlaC M li
Mm t o y 34 M t U i l l I N t
] 1
1
J
] J
t
i totip 030139t Q a u t l u u by ad pciafcad b y * Jb 09/34/9S
000689
Q uantify C a lib ra tio n lUport 10X120-09 d ilu tio n s
C a lib ra tio n s Ci\X XO 0LTXX\W LCT-l.mncurruD\032im
Laa t a o d i f l o d i Moa May 34 0 i0 4 t4 9 1991
triatad i
Mon May 30 M ilO tSO 1991
a
ou 0301991 M a ti ta t o i b y and p r i s t ad b y k jh 05/34/91
000690
AM ELIA062498: FACT E q u ip m en t Log 99016
Q uattro II, #6448E
PAGE
DATE: 05/25/99
ANALYST: hoi, sah
STUDY NUMBER Tox-120. Tox-026 MATRIX: rat liver, monkey sera DESCRIPTION: dils.
FACT Method: cts--M -5.0________
Injection volume. 10 uL
Cycle time: 9 min. Electrode: Z-Spray_______
M obile Phase:
fA12.0m M NH4CH3C02
TN-A- or stndrd number (A ) 99012-10-7
Prepared (date/by):
(A) 5/21/99 ias
Expires:
(A) 11/99______
Store at room temp? [Y] or N
Otilen
(B )MeOH_______________ . (B) TN-A-2540 (B ) 5/18/99 drb (B ) 11/99
Guard column: None _________
Part number na
Lot number na
A nalytical column: keystone b c ta sil___________________________________________
Size: 2 x 50m m _________________ Type: c l 8____________ Sphere size: 5 um
Part number 055-701-2
Lot number: P7J20 Serial number 038126D
A nalytical param eters:
Drying gas (L/hrl: 300
ESI nebulizing gas (L/hrl 15
LC Flow rate: 300 uL/min. Back pressure: 70 bar
Sheath gas (L/br)J)
HPLC solvent gradient: 9min-2
Time (min.) 11 0.00________ 40
2) 1.0_________
3 4.5 51 6.5
%B
40 95 95
L 1 _______ _________
71 9
40_
end
000691
Tuning Method Report
Method:
C :\MASSLYNX\RATS2.PRO\ACQUDB\PFOS
Printed:
Tue May 25 15:22:53 1999
Page 1
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 15 5 0.2 150 250
2.98 -15 -5
150 251
Pressures
Analyser Vacuum Gas Cell
Rdbk
1.6e-4 4.5e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy.Ramp Lens 6 Multiplier
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0
2.0 ? 0.0 5 650
7
Set Rdl
15.0 15.0 30 ' 46 2.0 0.0 40 40 0 48 650
000692
tid S
_______________________________________________ -
M asslynx - Sam ple L ist
Sample L is t C:\M ASSLYN X\rats2.PR O \Sam pleD B\052599a.SPL Printed: Tue May 25 15:16:14 1999
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42
43 44
45 46 47 48 49 50 ' 51
v 53 54 55 56 57 58 59 60
F ile Nam e
052599001 052599002 052599003 052599004 052599005 052599006 052599007 052599008 052599009 052599010 052599011 052599012 052599013 052599014 052599015 052599016 052599017 052599018 052599019 052599020 052599021 052599022 052599023 052599024 052599025 052599026 052599027 052599028 052599029 052599030 052599031 052599032 052599033 052599034 052599035 052599036 052599037 052599038 052599039 052599040 052599041 052599042 052599043 052599044 052599045 052599046 052599047 052599048 052599049 052599050 052599051 052599052 052599053 052599054 052599055 052599056 052599057 052599058 052599059 052599060
F ile Text
methanol blank methanol blank tox120, rbl04199-wblk-1 tox120, rbl04199-lblk-1 tox120, rb!04199-5ppb-1 tox120, rbl04199-10ppb-1 tox120, rbl04199-25ppb-1 tox120, rbi04199-50ppb-1 tox120, fbl04199-100ppb-1 tox120, rbl04199-250ppb-1 tox120, rbl04199-500ppb-1 tox120, rbl04199-750ppb-1 tox120, rbl04199-1000ppb-1 methanol blank tox120, rti, 3M -g1-250 ppb-ms, 4/19/99 tox120, rtl, 3M -g1-250 ppb-msd, 4/19/99 tox120, rtl, 110F-g1-250 ppb-msd, 2/22/99 tox120, rtl, 110F-g1-250 ppb-msd, 2/22/99 tox120, rtl, 106F-g1-250 ppb-ms, 2/22/99 tox120, rtl, 106F-g1-250 ppb-msd, 2/22/99 tox120, rbl04199-250ppb-cc-4 methanol blank tox120, rtl, 81 M -g 6 ,4 /19/99, d1000 tox120, rtl, 8 2 M -g 6 ,4/19/99, d1000 tox120, rtl. 8 3 M -g 6 ,4 /19/99, d1000 tox120, rtl, 8 4 M -g 6 ,4 /19/99, d1000 tox120, rtl. 8 5 M -g 6 ,4 /19/99, d1000 tox120, rbl04199-250ppb-cc-1 methanol blank tox120, rtl, 8 6 M -g 6 ,4 /19/99, d1000 tox120, rtl, 8 7 M -g 6 ,4/19/99, d1000 tox120, rtl, 8 8 M -g 6 ,4/19/99, d1000 tox120, rtl, 8 9 M -g 6 ,4 /19/99, d1000 tox120, rtl, 9 0 M -g 6 ,4 /19/99, d1000 tox120, rbl04199-250ppb-co-6 methanol blank tox120, rtl, 9 1 M -g 6 ,4 /19/99, d1000 . tox120, rtl, 9 3 M -g 6 ,4/19/99, d1000 tox120, rtl, 9 4 M -g 6 ,4/19/99, d1000 tox120, rtl, 9 5 M -g 6 ,4 /19/99, d1000 tox120, rbl04199-250ppb-cc-3 methanol blank tox120, rtl, 1 7 M -g 2 ,4 /19/99, d25 tox120, rtl, 1 8 M -g 2 ,4 /1 9 /9 9 , d25 tox120, rtl, 1 9 M -g 2 ,4 /19/99, d25 tox120, rtl, 2 0 M -fl2 ,4 /1 9 /9 9 , d25 tox120, rtl, 2 1 M -g 2 ,4 /19/99, d25 tox120, rtl, 2 2 M -g 2 ,4 /19/99. d25 tox120, rbl04199-250ppb-cc-11 methanol blank tox120, rtl. 1 7 M -g 2 ,4/19/99 tox120, rtl, 18M -g 2,4/19/99 tox120, rtl, 19M -g 2,4/19/99 tox120, rtl, 2 0 M -g 2 ,4/19/99 tox120, rtl, 2 1 M -g 2 ,4/19/99 tox120, rtl, 2 2 M -g 2 ,4/19/99 tox120, rbl04199-250ppb-cc-2 methanol blank tox120, rtl. 4 1 M -g 3 ,4/19/99 tox120, rbl04199-wblk-1
________________ ____________________ _ Pagel
IMS File
In let File
9M IN-PFO S-PO AA -513-613
9M IN -PFO S-PO A A -513-613 9M IN-PFO S-PO AA -513-613
9M IN -PFO S-PO A A -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M 1N -P F O S -P O A A -513 -6 1 3 9M 1N -P F O S -P O A A -513 -6 1 3 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613
9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 v 9M IN-PFO S-PO AA -513-613 9M 1N -P F O S -P O A A -513 -6 1 3 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA-513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613 9M IN-PFO S-PO AA -513-613
9m in-2 9m in-2
9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2
9m In-2 9m ln-2
9m ln-2 9m ln-2 9m ln-2 9m in-2 9m ln-2 9m ln-2 9m in-2
9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2
9m in-2 9m ln-2 9m in-2 9m ln-2 9m In-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9m in-2
000693
\ \ f e s f a _________________________________________________
Masslynx - Sample List
Sample List: C:\MASSLYNX\rats2.PRO\SampleDB\052599a.SPL Printed: Tue May25 15:16:141999
File Marne
61 052599061 62 052599062 63 052599063 64 . 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070
File Text
10x120, rbl04199-lblk-1 tox120, rbl04199-5ppb-1 tox120, rbl04199-10ppb-1 tox120, rbl04199-25ppb-1 tox120, rbl04199-50ppb-1 tox120, rfel04199-100ppb-1 tox120, rb!04199-250ppb-1 tox120, rbl04199-500ppb-1 tox120, rbl04199-750ppb-1 10X120, rbl04199-1000ppb-1
Page 2
MS File
9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613
In let File
9mln-2 9mln-2 9min-2 9mln-2 9min-2 9mln-2 9min-2 9min-2 9mln-2 9min-2
000694
S Izs
________________________________ ___
S c a n n in g M athod H a p e rt
Method!
C:\MMSLYXX\llAT82.Pll0\Acg00B\9MXN-proa-PafcA-513-613
U s t Modified: Tue Kay 23 10:39:4S 1999
Printed:
Tu May 25 13:19:29 1999
tolvant Delay ( alna ) :
0.00
Punction : 1
M M of 1 Maaa Pair ( u p - >
Inter Channel Delay ( Seca ) : Span- ( Daltona ) : Start Tina ( Mina ) : End Tina I Mina ) Repeats :
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs) Coll Energy (aV)
Cone ( V )
1'
413.00 169.00
0.30
30
15
Punction : 2
M M of 1 Maaa Pair ( ESP- )
Inter Channel Delay ( Sacs ) i Span ( Daltona ) : Start Tine ( Hina ) : E nd Tina (*Mina I : Rapeata :
Channel Parant Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Sacs)
. Coll Energy
(SV)
Cone ( V )
1 313.00 169.00 0.20
30
15
Tuna tion : 3
M M of 1 Maaa Pair ( ISP* )
-
Inter Channel Delay ( Seca ) : Span ( Daltons ) t
Start Tina ( Mins ) : End Tina ( Mins ) 3
Repeats : Channal Parant Daughter
0.03 0.00
0.00 9.00 1 Dwell
(Sacs)
Coll Energy
(aV)
Cone ( V )
1 613.00 169.00 0.20
30
15
Tunotion : 4
M M o 1 M a a Pair ( ISP- )
Intar Channal Daisy ( Sacs ) s
Span ( Daltons ) : Start Tina ( Mins ) t End Tina < Mins ) s Repasts t
Channal Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Seca)
Coll Energy
(aV)
Cone ( V )
1
499.00 99.00
0.30
45
60
function > S
M M of 1 Maaa Pair ( ESP- )
Intar Channal Delay < Sacs ) : Span ( Daltons ) : Stsrt Tina ( Mins ) t End Tina ( Mina ) i Rapeata t
Channel Parent Daughter
0.03
0.00 0.00 9.00
1 Dwell
(Saca)
Coll Energy
(aV)
Cone ( V )
1
427.00 0.00
0.30
40
60
Pag. 1
000695
FACT: DATA REVIEW SUMMARY FORM
Date:
Analyst
FACT Study Number 'y j ' k f T
Matrix
Time Points Dilutions?
______
0
Analyte 1:
/ k & 9'
Aecentahfa linear Renee: S T j~g -
LOO for ds data se t
S ~ lS /y6
Re-evaluated ffardifferent range?
Analyte 2:
A nSf` 3
Acceotable Linear Range:
" 'iS jW J
LOO fix'this data set
Re-evaluatedfor differentrange?
sS _
Analyte 3:
/h /7
Acceptable linear Range: vT< -- W /S /h
LOO for this data set
Re-evaluated for different range?
Analyte 4:
r*oj
Acceptable Linear Range:
LOQ for this data set
Re-evaluated for different ran^e? j \ J *
Analyte 3: Acceptable LinearRange:
LOQtr this data set *%0"GVWIURBvIO QZDjCaCQCTmumjmi
\ Analyte 6: \ /
te i Acceptable Linear Range: ' LOQfor tbis data set \ Re-evaluatedfor differentrWe?
________________________ V - _____________
Analyte 7:
\
Acceptable LinearRange:
\
LOQ for this data set
\
Re-evaluated for different range?
\
\
000636
&
oo
(? u "I
o -SZ. -5 ^
N00Nnt*0
i f I I I H H f l H I C4
I saaaasaaaai sslssss
^3 -"tt
rirM OhO A
t<-.
?1
2
i IIIn H t H v n
aaasas aaaaaaa sSsS 1 H H 1
A No
NmO OWWHMAWAfOkW
N8
H OH nniHi wA'On V
H1H_0f_0#f01s
m t4
Wfl*WNh 1H WWW
VAAAA nMm4 0kOAW0tA
nAO iONoAOo AA rAA*HAo rfAiOrNiOp HiOANONN AA rPOwtA-AOmoOooOoo Oon
vA vA A nn
OMO ooooooo loeeeoi
SWAr*AJoNhoNoo^NoMnf
ntfiiAAAAA AMAAAA AA ' AVAAAA
IHMOHnAptH
r t N A HrtMNOAPAI HH
AHMAHttttnH H A A HH Nh AAAAWH
HlthMl .fcWMWi ' "
1 t U tj U I
|S ..&..............!.................................. _..... ................*....... S *ii3 3 3 31U33_^_ -
3 3 8 ii!l 5 3 !ii3 8 s3 ls8 ls2 Iil8 3 l3 lilsl3 3 l;M IIl!f
j)
OooOooOooOooOoo
OooooOooOooOOoo
oooo
oo oo oo oo
AAAAAA
33335
333333
vrvjU i i i i StStSB kiiIAAAAA
Ao mo ni nimi ni aS
SAAHASAAHASAAAH.AAAHSAASAHUHIUI NHAotiA0AHNiAmHNiAOmHNkAmar^tIIPu0i
A010A1001t0011NI k k * _>rt o m m m m m - 01 01 01 01 01 01 f
SSSN SN U
HHrt4 I NSSSSN*VWsWNWS ffit
01 0k 0k 0 01 01 U H 4 H H H H I
01 0k 01 0 0k 0k N
ssssssU0 0) 0k 01 01 0 I
0 1 0 0k 0k 0k 0k U
0k Sr-tHHrtjw^* Ht
ftftftft
2g2aSn.&saLsAi 1".t.".t.7.t..M.. S 35+S& S A m * * &S^HMomJ'IdJ11I4oAAMHf1f*fAAoHMi4TAMoAiAoHAiAHo?iAAHo;AiO#Ah0m4A0AhNm3mQH04hAM)r4A0HAhM3O04A4h-M)tH4MRHNxOII*HzNoOt.IhtHIHOwhOHHbJ**oWbMHnJoHWHh|*OmHa>AmNIo.
WWWA
BIAAAAN
. - . *o
A*A*A*A*AJ.AAI
*
AAAAAN
NNNNN eiene^
AAAAH4h
s
h
g
h
g
n
g
n
* 4HHHH.OH.
UMMMMJ**J*44343 `
b h MMh h
3si r..i.H..H...W...H...HNHoNHo.NHoNHo*NHo*acooNNo No No NO NO ONj
o N
o N
o N
o N
oo NN
eo o oo oo jN N N N N M
!
*
O O O O OO B
HH H 4hN N N N N S
N O NNNNNNA
*O A O
j0N A A A A A A N A ) H A H N A H 01 P - H
n
h
soHi 4MBmHhiHshHhNguNgkNnHnsJkhs*OI0..W03.HHjS*HH0aoH1oHJoaHotHHAaooHraOoH4t aAOoHH aJOorik-4o**Ot Ho00oIk1Hrooo0rIH4koo>00rIkkHoo40<0IkHk
43 43 434)
" Mh H
H
Qk k k k k k k k goooooooo` S o o o o o o o
J N N N N N N N N N N N N N N N
fS fl SI _.x m x x v x x x x x k x I j x x x x m m S x x x x x x S xK xX xX xX x 4s ?
a s 4Q>O 4Ol0t i480 IOJO4 UO1Q0 QO >0O4 UO<O OM <OUO4 49P4 OMOW 0O4Ol 0O0U OlOk )O4Q} kQi0|
g g gSg S
41 AJ AJ U U *4
f8888S88
fgggggggg
|0 4 l0 0 4 l 4 4 tf
iSSS
41 AJ 4J 41
3O0001kO0OOkkOOOOkkOO0OkkmooOOkkoo00wk1f0oO0-kkOOo0OkkOoOOOkkkHoo0OkkHvo0O-kitNO0Okk-O0OAkk-<Ii4MkkA0AoiH0AkoHOrAokH0AAokMOAoOkN0AoHiN0AokNNOAoknN0Ao<kN0oArkANo0AkNOWOOkkOONOMkkfcc<
IINA NA NmNa 0>m
iAA OO
A A I I a a a a a a a a a a a AA*
>OoII>Nm
Nm
'
iII
N A
N A
N A
N A
N A
N A
N A
N A
N A
N A
N A
NNf AAH
o o o o o o o o o o o o O<
HNnoAWhwAOHNnAWham(
No0kmo0kfio0kj-ipo0N1)noOnkno0okAcOojkpo0koor0k B--A->kOOokHoO-kNoO-kAoOkOO--AO-W-O--PoO-kO-0o-kA-<t-O-o-r-o-I Mkfo0<kmAOok oOmkoOmmkoOtAtkrAoO-kcAoOoAkooOWkkoOOWkorOAWkoONWkoOrtkcU^fl
Om10Om10o
Ok Ok Ok
o
Ok
O
Ok
o
Ok
OkCk O 0k o 0k 0k
mm >mmknm mmmminmmmmmmmum m AA A A A NN NNN N N N N mm *mm mm mmm m m m m m m
Oo ooooo<oooo<oo ooooooooooooooo o o o o
i 0k 01 Ok Ok Ok Ok Ok 0k 0k t !0k OkOk Ok OkOk O k O k O k O l O l O k O k O k C m m m A A A AU
I N N N N N N N N N f N N AN N N N AAA
mOk mOk mOkmOk
Nm mN
OO
Nmo
moN
iwr-oAOHNAvAWACtComv-ii
NOC
7%.
<D
C> O O
o
a
* 1
e
L
3
XXf
Anali 062499: quantlatad and printad b y boj 5/28/99
J a u i* u m h (
*uiui m uiV uu><(Mu
9 10 40 40 40 40 40 40 40 40 40
910O 4O0 4O0 4O0 4O0 4O0 O40 O40 O40O10
O IA tt j0 b lA W M H O (0
:HO(oa>Joiv*WNMOttBg
ilU U U M M >
itMHOWM^nUtBWMHOWO^aWAi
m tuut> 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
M H M JtO l lU K jU JlU tU tU l i iU lM n U ltM M ll )N > M M M M N M M N N N M N N M M M N M M M M JIU U IU IU IU IU IU I t IU I tU I Ib lU llN IU llll
tMuMMuKu) w u <0 m
OOOOO OOO OOOO
M M mM MuiMm M(p Mw Mc p Mw Mw M0MmMu UMUMIMMMl
4P u* ui ui m 10
10 4M U U U t U t U t U t t u u t m
ut
Ow Ow O10O40O(0O(0Oin M NNM M M M U t Ut ( 0 040 t u u
> 4 0 40 40 40 40 40 40 40 40 40 40 40 40 40 1 0 0 4 0 4 0 4 0 4 0 4 0 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 ( A 40 40 ( 0 ( 0 10 40 40 40 40
O O O O O O O O O O O 0-0 O O O O O O O O
}l0l^UMHO(Da'JAU*UNHOVO>J
uO wO Ou Ou Am *u
O u
O u
UO MO KO)
OK IOMOMOMOM
O
O
O
OOOO
OOOO
O 40 O 4 n u 0 U K t H 0 4 0 W A U * U U
O O O O O O O OOOO MMO O OO O M O 40 CB J 04 40
rt rt rt rt r
SSS85
ft ft ft ft ft ft ft X ft rt rt rt rt rt rt o p o p o p o aopooooo_
gs
ft ft ft ft ft OOOOO
ftrrrtftftftH rtftftftrrft
O O O O O O O O O O 0.0 O
X
X
X
X
X
X
K
Q
X
X
K
X
XX -
X
g
t
Si r .X....X....X....X...X XH MX HX HX HX HX tf trXHXH XH XM XH XM
N 0
U 0
N 0
N 0
M 0
0 N0 N9s0
M 0
M M N N N M IM N M M 0 0 0 0 0 0 9 OOOO
M O
0 MNNMMN g OOO OO O
- -
* * * % * * * %
.......................... o
H H 1 M! HS' H0*0H* tHr<4
H H H H K H t| tr tr tr tr tr tr rt t
00004o o o o o o*> I
6 Xa a a M M M M M M M t
Htr
Hft
Hrt
Hrt
f* ti
rt
rt
H er tr
rt
H rt
H rt
* rt
h ft
H rt
t
O t t t t t p h0o *h h% h* h^ h m ia o * % * *
a- 9 *
H N N N 4 J H H }fH N N M H H H K 'H V t0 l0 l0
t%r tr r1t fHt rXt Xrt Xft tr Htr Krt HrtNftHrtHrt
HHHPHHH
g o .****
h0 0
5 2 ........
X h a a a 0a
SSSSS! SKSSSS g g SI 4 0 40 40 40 40 40 4 0 M
- > i p | p 40 40 40 40 j
4 I0 N M U 4 H 40 ? w p t r o ' u S S a.H
I i I I iT i
MlO 0 0 1 0 0 0 IP M N M N m m
lilil
I I I IT
WUol004001004O04
mm g 04 04 04 04 04
0t SHSHSHS HNH. HS n 40 40 40 40 40 40
fIf HWH Ms SH O 40 40 40 40
i a a 4040 a
I 40 40 40 40 40
ftft
U4 U 414 U U4 U4
OOOO
OOOO
0000
AM nMaHoH. Ha OOOOO OOOOO OOOOO
aH Ha Ma Ha Ha OOOOO OOOOO OOOOO
Ht r cHr Xc rHt rXc rHc rHt r t r t r fat H H PH H H H H H o o o o o o o g g I
40 40 40 40 40 40 40 40 40 40 40 40 40 40 I I I I IT I M UNM UHI 0 0 0 0 9 70'
ilf?
ft
| Ck in r s
- f ih i
iy
tit2 s ? "
I K. B * B
-3
~ 3 3
H M>
40 X 40
Ti 56 pm
euN M U M o JH M H H U a b h o w h ip
0 taU>UMPUM0 (0
auuuuaui UCt A H O H N
u t w o u u* m u t
H IO H B M O U A U I0 U M O
<J Ol
J*I0 U N H
hm m m hh
WOAUUMU U A H ^ B 0(1
UHMHH MAH0UA40
HH H
N *JH H I0 C H
NH
H 00U 0A H
O0M 0AH0
A H H H *JH U
O in *J O O
% gSS&gSg gg&5?gS 5
till
M
UUOA MUON NOAI AW OO
BC. . . . 1 g^a a a
H B 4 IM H H U U l-jM U lM I' M u a a P i- 4 1
as.
t*U lU lU IU IU IU M JJIO lU IU M 0U IU
*00 U00 000v00)040f0*U*rM<Ao4Uua0wa*uw*ia0aA00a*a* 0HJ
M
ilk
W
4P H
WM 0
gg
1 H
*m m m h h M m
9 40 <D 04 HI
40 ! W W M
N 0 0 H U > IH
0 U * iM 0 N 0 U a O A O N N
4 0 O W V iU 0 U l4 0 U iH a A A * l0
1g&ggffggg g g g g g g g
irv < ?
11
i r UT M l 1H H U U " St
v/
0
8.
3
m
IQ
?2
3
?
Quantify Ccaound Summary Raport TOX-120 Xat Livar Dilution
8ampia U a t : S:\PACT-1.PRO\SamplaOB\051499a
Last aodifiod: Mon Hy 17 14:14:20 1999
Mathod:
S : \ P A C T - 1 .PftO\>totb&B\051499a
Last aodifiod: Mon May 17 14:23:30 1999
Job Coda:
Printed:
Tu M ay 1 08:22:40 1999
Compound 2: M463
4 Mua 1 051499041
2 051499062
3 051499063
4 051499064
5 051499065
6 051499066 7 051499067 9 051499068
9 051499069 10 051499070
11 051499071
12 051499072 13 051499073 14 051499074 IS 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 051499080
21 051499081 22 051499082 23 051499083
24 051499084 25 051499085 26 0S1499086 27 051499087
28 051499088 29 051499089 30 051499090
31 051499091
32 051499092 33 051499093
34 051499094
35 051499095 36 051499096 37 051499097
38 051499098
39 051499099 40 051499100 41 051499101
42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114
Saaplallst Taxt MsOH Blank MoOH Blank .
Tox-120, RBL04199-H20Blk-2 4/19/99 Tox-120, RBL04199-LBlk-2, 4/19/99
Tox-120, RBL04199-10ppb-2, 4/19/99 Tox-120, HBL04199-2Sppb-2, 4/19/99 Tox-120, *BL04199-S0ppb-2, 4/19/99 Tox-120, RBL04199-100ppb-2, 4/19/99 Tox-120, RBL04199-250ppb-2, 4/19/99 Tox-120, RBL04199-S00ppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99 I M S Blank
Tox-120 23M G2 / Dl:23 Tox-120 24 62 / Dl:25 ' Tox-120 2SK 62 / Dl:25 Tox-120 26M 62 / D1:2S Tox-120, RBt04199-2S0ppb-CCl, 4/19/99
M.OH Blank Tox-120 33K 63 / Dl:23 Tox-120 34M 63 / Dl:25 Tox-120 35N 63 / 1)1:25 Tox-120 34M 63 / Dl:25 Tox-120 37M 63 / 01:25 Tox-120 38K 63 / D1:2S Tox-120 39M 63 / 01:25 Tox-120 40M 63 / D1:2S Tox-120, BBL04199-250ppb-CC3, 4/19/99 MeOH Blank
Tox-120 27H G2 / 01:10 Tox-120 29H 62 / 01:10 Tox-120 30M 62 / 01:10 Tox-120 31M 62 / 01:10 Tox-120, BBL04199-250ppb-CC4, 4/19/99 HaOB Blank Tox-120 4211 63 / 01:10 Tox-120 43K 63 / 01:10 Tox-120 4SM 63 / 01:10 Tox-120 46K 63 / 01:10 Tox-120 47K 63 / 01:10 Tox-120, RBL04199-250ppb-CC3, 4/19/99 MeOH Blank Tox-120, HBU)4199-H20Blk-2, 4/19/99 Tox-120, RBL04199-LBlk-2, 4/19/99 Tox-120, RBI,04199-- 5ppb-2, 4/19/99 Tox-120, RBL04199--10ppb-2, 4/19/99 Tox-120, BBL04199-25ppb-2, 4/19/99 Tox-120, BBL04199-50ppb-2, 4/19/99 Tox-120, BBL04199-100ppb-2, 4/19/99 Tox-120, RBl,04199-250ppb-2, 4/19/99 Tox-120, RBL04199--500ppb-2, 4/19/99 Tox-120, BBIt04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99
Typ* Analyta Analyta
Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
Analyta Analyta
Analyta Analyta
Analyta OC Analyta Analyta Analyta Analyta
Analyta Analyta Analyta Analyta
Analyta QC Analyta Analyta Analyta
Analyta Analyta QC Analyta
Analyta Analyta
Analyta Analyta Analyta QC Analyta Analyta Analyta
Standard Standard Standard Standard Standard Standard Standard Standard Standard
Std Cono
XT
5.56 11.10 27.80 55.60 111.00 278.00 * 556.00
833.00 1111.00
278.00
.5.307 5.306 ` 5.307 5.307
5.306 5.308 5.308 5.308
5.31S . 5.307
5.306 5.307 278.00.
278.00
5.308 5.306 5.307 5.307
278.00
5.306 5.307 5.306
5.307 5.306
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
Aras
Kaoponaa Cone, rings %Dav
613 637 509 '
473
612.731
636.927 509.037
472.806
48 49 47
44 44 35
64 43
48.048s 48.381
46.922 43.598 43.676 34.651 44.313
42.794
1080 1367
1207 1023
1079.684 1367.145 1206.705 1022.573
66 66.125 83 83.086 50 50.209 63 63.197
55 55.155
bbx
bbl bbX bbl
MMX MMX MMX MMX lfifX MMX MMX MMX
bbl bbl bbl bbX
MMX bbX MMX MMX MMX
Pag 2
Soup 020199, Xaviaaad and quantitated by drb 8/17/99
000699
Q u an tify Compound Sunaary la p o r t toxl20, r t l
ampi. Lit: C :\ M M L Y N X \ r t 2 .PKO\bnplaDB\OSZS99a
Laat modlflad: Wd May 2 07:17:2V 1*0*
Mathod:
C:\MMaLytOC\rata2.VlW\MathOB\OS2S9Sa
Laat ediflad: ad Ma y 2 07:10:17 1900
Job Coda:
Printad:
ad May 2 00:20:32 1000
Caopound 3: M613
1 HtM 1 052599001
2 052599002 3 052599003 4 052599004 5 052599005 6 052599006 7 052599007 9 052599008 9 052599009 10 052599010 11 052599011
12 052599012 13 052599013 14 052599014 15 052599015. 16 052599016 17 052599017
18 052599018 19 052599019 20 052599020 21 052599021
22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027
28 052599028 29 052599029 30 052599030 31 052599031
32 052S99032 33 052599033 34 052599034 35 05259903S 36 052599036 37 052599037
36 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043
44 052599044 45 052599045 46 052599046 47 052599047
48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 052599056 57 052599057 58 052599056 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070
Saaplalist Tant
nathanol blank
Mathanoi blank
toxl20, rbl04199-wblk-l
toxl20, rbl04199-lblk-l
toxl20, rbl04199-5ppb-1
toxl2Q, rbl04199-10ppb-l
toxl20, rbl04199-25ppb-l
toxl20, rbl04199-50ppb-l
toxl20, rb!04199-100ppb-l
toxl20, rbl04199-250ppb-l
tox!20, rbl04199-500ppb-l
toxl20, rbl04199-750ppb-l
toxl20, rb!04199-1000ppb-l aathanol blank
i\fc?
tox!20, rtl, 3H-gl-250 ppb-as 4 / 1 9 / 9 9
| | eC
toxl20, rtl, 3K-gl-2S0 p p b - u d , 4/19/90
toxl20, rtl, 110r-gl-25O ppb-ma/, 2/22/99
toxl20, rtl, 110r-gl-250 ppb-aad, 2/22/09
toxl20, rtl, 107-gl-250 ppb-ma, 2/22/99
toxl20, rtl, 10F-gl-2S0 ppb-aad, 2/22/00
toxl20, rbl04199-2S0ppb-cc-4
nathanol blank
toxl20, rtl, 01M-g(, 4/19/99, dlOOO
toxl20, rtl, 02H-g, 4/19/99, dlOOO
toxl20, rtl, 03M-g, 4/19/99, dlOOO toxl20, rtl, 04M-g, 4/19/99, dlOOO
toxl20, rtl, SH-g, 4/19/99, dlOOO
toxl20, rb!04199-2S0ppb-ce-l
nathanol blank
toxl20, rtl, 0K-g, 4/19/99, dlOO
toxl20, rtl, 7M-g, 4/10/09, dlOOO
* toxl20, rtl, (8K-g6, 4/19/99, dlOOO
toxl20, rtl, 9K-g, 4/19/99, dlOOO
toxl20, rtl, 90M-g(, 4/19/99, dlOOO
tox!20, rbl04199-2S0ppb-ee-S
nathanol blank
*
toxl20, rtl, 91M-g(, 4/19/99, dlOOO
toxl20, rtl, 93M-g, 4/19/99, dlOOO
toxl20, rtl, 94M-gS, 4/19/99, dlOOO
toxl20, rtl, 95M-gi, 4/19/99, dlOOO
toxl20, rbl04199-250ppb-cc-3
nathanol blank
toxl20, rtl, 17M-g2. 4/19/99, d2S
toxl20, rtl, 18M-g2, 4/19/99, d25
toxl20, rtl, 19M-g2, 4/19/99, d25
toxl20, rtl, 20H-g2, 4/19/99, d25
toxl20, rtl, 21M-g2, 4/19/99, d2S
toxl20, rtl, 22K-g2, 4/19/99, d25
toxl20, rbl04199-2S0ppb-cc-ll
nathanol blank
toxl20, rtl, 17M-g2, 4/19/99
toxl20, rtl, 18M-g2, 4/19/99
tox!20, rtl, 19M-g2, 4/19/99
toxl20, rtl, 2QM-g2, 4/19/99
toxl20, rtl, 21M-g2, 4/19/99
tOX120, rtl, 22M-g2, 4/19/99
toxl20, rbl04199-250ppb-cc-2
nathanol blank
toxl20, rtl, 41M-g3, 4/19/99
toxi20, rbl04199-wblk-l
toxl20, rbl04199-lblk-l
toxl20, rbl04199-Sppb-l
toxl20, rbl04199-10ppb-l
toxl20, rbl04199-2Sppb-l
toxl20, rbl04199-50ppb-l
toxl20, rbl04199-100ppb-l
toxl20, rbl04199-2S0ppb-l
toxl20, rbl04199-S00ppb-l
toxl20, rbl04199-7SOppb-l
toxl20, rbl04199-1000ppb-l
Typ Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta
Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta
QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta
QC Analyta
Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
Std Cono
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
278.00
- 278.00
278.00
278.00
27B.00
278.00
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
Kt
5.795 5.797 5.765 5.808 5.784 5.782 5.776 5.767 5.788
5.782 5.778 5.784 5.772 5.765 5.767 5.767
5.792
5.782
5.795 5.781
5.813 5.812 5.791 5.788 5.815 5.783 5.785 5.782 5.789
5.741 5.807 5.806 5,775 5.786 5.790 5.765 5.787 5.786
Aran
Cone. Flags %Osv
3 1.54 KM 5 8.66 MK
14 32.23 bb
33 82.54 bb 53 137.72 bb 116 304.34 bb 221 587.57 bb
318 848.84 bb
369 985.61 bb
-72 -22
16 48 24
9 6 2 -11
105 274.93 bb
118 310.32 MM
104 273.67 M M \
92 241.88 M M 2
102 268.45 bb < w e r e c l
120 315.53 MM J
101 266.49 HM
-4
112 295.72 M M
6
112 295.27 M M
6
100 262.77 M M 5 8.69 bb
-5
76 199.37 MH
-28
. 36 89.73 M M >
28 69.25 b b :/ i 1
33 84.01 bb *) E i i fcru
30 73.71 b b <
39 96.92 bb )
29 72.66 bb >
81 210.54 M M
" 24
27 66.29 bb
9 17.56 bbX 216
x
7 11.87 bbX`
7
13 29.34 bbX
6
11 23.51 bbX -58
41 103.06 bbX
-7
87 227.94 bbX
-16
150 398.05 bbX
-28
204 542.63 bbX
-35
252 669.29 bbX
-40
Vag 3
Uc. oil
Anali. 0(2490: quanti atad and printad by ho] 3/2/90
000700
sP
Ift i I
9
t S
0 0 0
0LA
MKAAA A( lA A U M H f
It ffllAV Ui gMiHeitt tDMAf^lAA AMAAAUANA AH AOAlAll
IAU M H O VA>JA AU M H *
> 0000 lu m u ib i (M M M M i m m m ui > U M O U > tO
) 10 40 0 IA > 0000
IAAA A
M P lA
e o o O O O O O O O OOO OOO O O O < O O O O
UO(A AAN M
U----U--MU AAAAAAAAAAUJ
IA Al
AIA AAK)
IA IA 4AIA IA AAAAAl AIA
IA l AIA
t n (a I A I A _ _ _ _ _ _ AIM M M M M M M A IM
M UUiA IA IA U UH* CAIA IA IA IA 04 IA Ui Ui IA C IA IA IA IA4I'A IA *04' IA" I"A *I"
40 IOIO40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 If 40 40 40 40I 40 40 40 40 40 4
40 IO40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 If OOO O O 0 0 0 0 0 OOO OOO O O O C
40 40 40 40>40 40 40 40 40 4 O O O o> 0 0 O O O I
A A A 04 04 01
AAU
O IO A
IAIAIAIAC(AACUA>01 (A
*A A
M H O I0 O < J A IA *U M M O
WWW! 40 00 J <
IA M (A 40 40 O u
IA M (A 40 40 O u
OOO IA IA (A MMM (A IA IA
40 40 40 40 40 40 OOO uuu U K1K
OOOO IA IA IA IA MMMM |n <A<AtA
40 40 40 40 40 40 40 40 OOOO MM MM O 40 CDI
OOO O O O O O O O
CA4AIA IA IA (A 4AIA IA 4A
M Al N M M M M M M M
IA IA (A IA CACAIA AIA IA 0* IA IH IA
10 40 40 40 40 40 40 40 40 40 40 40 40 40
40 40 40 40 40 40 40 40 40 40 40 40 40 40
0 0 0 O O O O O O O OO O O
K) A) Al N N N N H H H 01 IAUAAl> H O IO B J
O
O
i
A
U
OOO IA (A (A M Al Al IA IA IA 40 40 40 40 40 40 OOO
MHO
IA 40 40 O O 10
IA 40 40 O O
00 O IA IA MM IA AIA 40 10 4 0 40 40 4 0 OOO Oo o *4 04
O
IA 40 40 O o
O
IA 40 40 O o
O
t 41 < <
O
*"
rt rt rt rt rt 00000 M X X M I HHHM
Isssssssir rt f t f t f t f t ft X r t r t f t f t f t f T f T l
OOOOOOO
XXXXXXX
Bm m m m m m m S oooooooa
5 ilil _*I s s s s s s s shi r t f t r t f t f t ft X f t f t f t f t f t f t X 1f1t f t f1t f t f t f t f t g f t f T f t f t r r r r r t f t O O O la m a s x S k S S S x
`T H H H H H P H M H M M M VM M
AB M M M M M M >5 O O O O O O
f t MMMMMM 9 OOOOOO
B9
M o
M o
M o
M o
M o
M o
M o
ft a
o
M0 M0 M0 0M 0M 0M 0M 0M 0M 0M 9fOt Of?t f0ftt*
**H H U H N H H
8O' f t f t rr f t rt f t W f t r t r t r t r t r t l l^^***ftft*ft*ft-*ft*ft g o -------------------------------g o ---------------B2 .............B s s s p p s s r TIf H f --
.*1 H N H H
t O' IT f t f t f t f t f t -ip H H H H H
KHIBOOOAlr
f f f f . T I I 7 I I I IIM M M M M 8 e a 9 i0*i0oi rOr'_O 40 10 40 40 40 40 ssssn W S S S S
I 10 40 40 40 40 40
N I) l iQ
IA M Al Al M M M
$ .................................. V A *A A A * ffS S S N S S
IH H H H H H O 40 40 40 40 40 40 ft S S S S S S i 40 IO 40 40 40 40
S AAAA
I H ftftft 40 40 40 40
I 40 40 40 40
IA * 01 04 04 04 40 40 40 40 40
MO O O O O |A 04 04 04 04 04
1 ft*ft*ft*ft*ft* fftt S40 S40 S4 0 S40 S40
I 40 40 40 40 40
tr *ertr o* tr tro' tr XtrXcrXo'erer fftt
^ p H f t f t f t f t f t f t ***** ` OOOOOOOO
XaJ Mafo| o44T 1T0
n r 7 7 r ft* ft* u o a fttO I I O ft* ft* ft* ft* M M " I I I I In IA
MMMMOO
4 0 4 0 40 40 40 40 40 40 40 40 40 40 40 40 40
ft*-J(AA>ft*tAAft*
O' O ' O ' I I I I l I H H ft*
M 10 40 40 40 40 40 4 0 40 40 40 40 40 M 40 40 40 40
ft* 10 40 40 40 40
fcfcft&fcfc
IA CAIA 4A4AlA
aM aH dH uH
OOOO
OOOO 0000
aftnHaMa ffttft
OOOOO OOOOO
OOOOO
aft*aft*aft*aP aft* O0 0O O0 O0 O0
OOOOO
M SM M HV SM SS40S MMNMNU M V. Al M 40 40
W V S ID 10 40 40 40 40 40 40
4?
I > i|U
im i
III?
ft* ft* CD CA M f t * ftU (R < JM lA N H ft*U 049ft*CA 4ft*CA
OOOOOAAM U OOOOOOOOO
M vl CD O O
M 4
O O
( A ( A CA I A I A t A I A I A CA (A IA CA CA CA CA CA CA CA IA
m m m m m m m n w * 1 0 IO 0 A i* J O O JO V M A M * I0 U N
M W UW MMMM M O O 0000 * UUOW HAM
M CD M
MM M M <J J 1 A CD CD od m
OO OO
00 00
ft* ft* m CA M f t * ft*M CAJft*CAM ft* t*M 0lCBft*CA'4ft*CA
O O O O O 0 4 C ft* CA 0000000004
CA t A CA
M M OI CD 40 O 0*0
CA ( A CA (A CACA CACA CA IN | A (A M M M M Al Al Al Al Al Ai M M CD 40 J Oft D U < l < i 40 J 0k CD CD * 40 W A M U 0 CA * O CA
CA CA (A CA CA CA I A CA CACA CACA CACA
`M M M M M M M M M M M M M M 0 0 0 0 0 * 0 0 4 CA 0 4 * J 04 1 * C A M M O O D C A M ft* CA 04 O 0 4 CA
CACACACACACACACACACACA
MMMMMMMMMMM <4CD0ICA<4<4CAO404CDM C B *4O tA -4O D 4O *M 40
i o m ca m m O 4O M 04O CA A M M U O O U C A *J < I O V f t
O 01 1 * l A I A CA 01 01 O ft* 40 M 4 0 * Oft Ak *
JU A M A l CD M ft* 4 0 U f t * H M H * 4 * jA ft* 0 0
{ A M O 4 (A 0|40I0M O O A A O 0< J< 0M < JO O
M ft* Al ft* ft ft M M M
40 ft* 04 M M O M * 40 O 0 k CA M 04 * 0ft 01 CA 00 J CD M O
* * w CA
m m m * * * . 2
I I I I I I t Ift*
U U X N ftW *A O VOAHXW AAO
311 370 367
279
ft* 0k A Oft
sm
t*l * * * (A Ol 01 CD O <J 04 01 U - J 40 40 * 0 01 04 * IO
* 0k 0k * 0k U
9 CA CA 0 4 0 4 04 *
O 04 O 40 40 04 * O ft* 04 M O 40
U 4 4 0 O * M f t * C A O 04 04 Ot M * 04
ft* ft* M ft* 9 * ft* ft* M ft* 0 4 * 40 M 4 0 * * l 0 f t * JV 0ft*IA 4A 40M M M *M
OOO
. 00 OOO 88%
M MMMM MU M* * *
CA (A O ft*
CA O * 40
0k a
0
A 40
0M
M 01
O 4A CD M C A 40
ft* * O ft* U O * A * 40 M J A CD M CD ft* Al O A 04 A CA 04 M
88888888888
M * * M (A M M U M * * 4*1 Iti
CD CD M * O *
O CA 0 4 CD CA CA (A
O M 4 0 O O 0 4 O 4 4 CA O O 0 4 O
O f t * O M 4 0 0 A Ok M O ft* O M 40 0 4 f l D C A J O O * U 4 | A CB O 4
88888888888888
4 0 CD 0 t M ft* CD M M ft* M 0 4 * *IA C D O O 40 4 -* * M O O
IA **C D O A 4O 0k*O O * M 4 0 IA 0 4 W ft* 0 4 O O O
S8888888S88
t- 1 0 0
- .
I
?r
0
"C5" r*
I tI ft* I ft* ft* M in IA M ft*ft*MMflU'ft*CD40
3
I
n< Vnn0 nnh
H
<H
9
Hi
V
0iOr t
f
f t
H wi
H
00
00
Na
H0
N
f ii i iiii
o*
s sssssssss s s s
OHHHHHOHOOO MOOOOH
m r* n N n n n N W N H 0 O h O A O 0 2 NP><monH0Nt' OoVAOrintf ft A 0 * n n f 4 o e H-1N H N N N K 1 AH
NV NN AA AO NN mA nA nA H0 AAnA OOAOOAAAAAA
1A 1A 0 1 A 1A 0 0 0 0 0 0
HA HO NA AA OH AA 0A AOAAOAA
0M00M00
n a a r- on AAAA0AN IIIIIII
nIAIn AAI AAI AAIAAIHAI.
DIPwlIlnH nH HIAIN NI ArINtI*HI4N!mH
2 2222222 2222222 222222222222 o o o o o o o 0 0 0 0 0 0 000HOOHOOOOO
f00Nm
in n noa a>A 0 O A N r* OlAHA A N A M A A HOAnPIOAHAAAA PA0A0AO H 0 O 0 N N N N O O A O
OAH0AWO HNtHHTN OOOOOOO IO A A A A A A
O000A0N NNHAHAH OOOAOAO
MHhNAAONrHNH
AOHNH0OAAAAA AOOOOAOAAAAA
IAAA0A00000
ss.... h h h h
.........._ _ J 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SSSSSS SSSSSS SSSSS
OT*0TJflTJtTJ*0
h U titih titi H n h h U U H H h U U U h ^ & h iU ih i
<r* "3
2*
AHNNS
<0 VT9 T)
3332
OOOOO OOOOO
OrjO O O O
OOOOO OOOOO OOOOO
OOOO OOOO OOOO
AAAAAA
AAAAAH AAAAA I
AA AA AA AA AA AA nI
A A A A A H AAAAA0
SSSSN.S 0 AAAAAA P
HHH I
HHHHHT
HHHI HHHHHH1
O O Nn Nn nN AN Aa
S.-s*S.N.'*s<
s s s A n n s s s n J SSSSS.S 000ft 0 0 0 0 0 0 1
JAiA'l
nnnnnnftOA O O n s n a
0 a i i i iS
N1 NI HA HAH0H*0OA
O <0O O A A A
:i y
AAAAAC4 AAAAAn A AnA H N A H N A h H H H I IJJ N
A Mh1 1 * 1 1 1 1 1 1
AAAAAAAAA
A Am kwh I IX O O A A A
........ A
AAAAAAAAA ZZHHOOA.
RRh h h h h J 90000HM 0 0 0 0 "
O O O O O O O O O ..
0 3
a0
5 a0 A
.y.1 SA A SA SH
X
H sH HSn iN Sn.n. x .ss
HaHaHNaaNnan^0nAIH,AA9fIlHAAAHIAASIHAAoINAAAI AA0HI0AANI(AA0I)AA0IPAA0*IAA0HI t . . OS OOOO O O O O O O O O O
H.p
H
H-OHA
H k
H A
H A
HH J A
H A
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
h
MHHUUUU *
sssssssssfssgssgsissssssssssssi ggggg^ggggggglO O O O O O CitiCiCitiCi
c2 OnOnOnOnOn OnOntOntOnkOntOnOnkQ<L0nk-n0knOknOknOknOknOk Oc0 k
S XX*MMMAJ. . H H H H H H H H H H H H H H H h HHHHHHHHHHHH,S h h H h h x h h h h h h h j HHHHHHHJS
IAsJs3
i*J J *J *J*J +J J 4J44I4J40 4141y 4JilililJy y0 y0 0y 0y 0y 0y 01 AxoJaoxJaoxJAoxJAoxJJoxJAoxJoAxJoAxJSAxJSAxJSAxJ
1 nnonnhnnnh
H>> N00 00n 000 mO Ow Oh ooO0 ooO0 oOH
1A 0101
AAA
A A A A vt00A A A
anAO
nOaA
nOaA
nOAa nOaA OnAa OnAa
AA nn
AO OA M
H
o
A A
N n0O
O o oo
A A AA AAAA AA
nA An
0 O A A
An
0A
i* O A A
nA
0A
A O< A< A(
An *
0At
0 A A A A
00 AA AA AA AA
nnn
AAA0 0AAA 0AAA nA nA An
OOO
9 n b
iIiiii>Ii1i)>illnAAAnA00 A00nAAnA AnoAAA
A A An oA
H N PIT A o r*O A
AAAA
AA
OOOO 000 O O
AAAA
AA
AAAA
AA
An
An AnAnAnnAnA
inn C4C
AAAAAAAA A
OOO OO
O^ n^ n9 A0 V0 A0 O0 n0 A9 A0OArA AAAA0A>AAAOrA *A9AA
------o--o----I--o--o------>--o--o--<--O O O O O O O O
A A tA A A A C A A A A A A A A
A A AA A A A A A A A A A A
m tnA A iCif4fiCi AAAA OOOO
>i}>OAAnnOAA o___o)1iAAonnAoAMc
AAAAAAAA nnnnnnnn AO AO AO AO AO AO AO AO
ihn
A A r- m A O
I w w 0A A A A A A n
>00 AA
O A
O A
OA
OA
O A
O A
0A 0A
AA A A A A A A A A
)A in
A n
An
An
An
nA
A
A
AA AAAA
OOOOOOOOOOO
33
Hnn000p
HO dH HN Hn H0 H0 HW Hh H# H0 nO nH nN nn n0 nA n0 f
A o n n n 0 A A r '9 A O H f
)A A A A A
c* o
o o o
A m X i a 023(91: quantlafcad and printad b y hoj 5/28/99
Q uantify C alib ratio n Raport to*120, r t l
C a l ib r a tio n : C:\9AMLTWC\rata2.VK3\CuxvaDB\0S2599a
L a a t a o d i f i a d : Wad a y 2 0 7 :5 9 :3 9 1999
V rintad:
Wad a y 2 0 1 :2 1 :0 3 1299
Van l
000703
Q uantify C alib ratio n (a p a rt toxl20, r t t
c a l ib r a tio n ! C:\MM9LTWC\rate2.P9O\CurraD9\052399a
L a a t a o d i f l a d : Wad May 2 0 7 :5 1 :3 1
P rinted:
Wad May 29 0 9 :2 1 :0 9 1
Pago 2
alia 09249! q u an tlated and p rin ted by boj 9/29/99
000704
Q uantify C alib ratio n ta p o rt toxl20, r t l
C a lib ra tio n s C:\M M SU N C\rata2.m o\CurvaD B\052599a
L a a t a o d l f l a d : Wad May 2 0 7 s S (:3 S 1999
V rlntads
Wad May 2 0 9 :2 1 :0 5 1999
000705
Q uantity C alibration Hapert toxi20, r t l
Calibration* C: \M*4IL.nOC\rata2.P*O\CoxvDB\0S2S99a
Laat modified: ad May 2 07:91*39 1999
Vrintad*
ad May 2 09:21:09 1999
a lia 092496* quantin te d and p rln ta d by bo j 9/24/99
000706
Q uantify C alib ra tio n K apert to*120, r t l
Calibration: C:\HASaLnoC\rats2.FKO\CarvaOB\032399a
Laat modified: Wed May 2 07:55:3 U M
Printed:
Wad May 2C 09:21:05 U M
Fan a
Amalia 062498 : q u a n tla ta d and p r in te d b y h e] 5 /2 6 /9 9
000707
Hathod Report
Method til: Last modified:
Compound:
Printed:
C:\HASSLWOC\rats2.PRO\MathDB\OS2599a.mdb Wad Kay 2 07:18:17 19990
1: P O M
Compound H u n Internal Rot.: Quantity Tracai Cane, of Standard*:
Usar RF Value: Usar peak Factor: Integrata Window Factor:
Peak Location
Retention Time (mina): Relative Retention Tima: Tima Window (mina):
Peak Selection:
Response
Response Type: Response:
Calibration Curves
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
Concentration
Obits:
Noise
Peak-to-peak amplitude:
Smooth
Smoothing: Window is (scans): Number ot smooths: Smoothing method:
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
Peek Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold:
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
PCM [Hono] TIC Cone. A 1.000000, Disabled 1.000000 2.000
5.010, Enabled 0.000, Disabled 0.304 Nearest
External Areas
Linear Exclude 1/X None
(0
'D i s a b l e d 3 2
30.00 70.00 4.00
10.00 Disabled 10.00
50.00,* Enabled 0.00, Disabled , 3.00, Enabled 0.00, Disabled
-
l a c o s / u l n _______________
Page 1
000708
Hathod K aport
Hathod fila: Last modified:
C:\HU3LnK\rats2.MO\XathDB\052599a.ndb Mad Hay 26 07:11:19 19990
Compound:
2 : H513
P rinted:
Mad Hay 2 0 1 :2 1 :1 9 1999
A hJ i *
Compound Nana: Internal Rtf.: Quantify Traca: Cone* of Stmndarda:
Uaar RT Value: User Peak rector: Integrate Nindow rector:
Peak Location
K513 (None) TIC Cone. A
1.000000, Disabled 1.000000 2.000
Retention Tima (mine): Relative Retention Tima: Time Nindov (mins):
Paak Selection:
Resnonse
5.531, Enabled 0.000, Disabled 0.233
Nearest
Response Type: Response:
Calibration Curvea
External Areas
Polynomial Type: Point of Origin: Fit Weighting: Axis Tranefornation:
Concentration
Linear Exclude
. 1J * Mona
Units:
Noise
Feak-to-peak amplitude: Smooth
64
Smoothing: Window alza (acea): Number of smooths: Smoothing method:
Baaellnea
Disabled 3 2
Join valley threshold: Reduce peak tailing: * Raise baseline maximum:
Peak SeDaratlon
30.00 70.00 4.00
Draw vertical threshold: Detect shoulders: Shoulder threshold:
Response Threshold
10.00 Disabled io.oo
Relative height:
Absoluta height:
Relative area: Absolute area:
50.00 Enabled 0.00 Disabled 3.00, Enabled 0.00, Disabled
___o l m I i
Uc o r h J il
Paja 2
000709
Method Report
Method file: Last modified:
Compound:
Printed:
C:\MAS3URM\rats2 .PR0\KetbDB\0S2599a .mdb Nad May 26 01:10:1? 1999a
3: M613
Ned May 26 00:21:19 1999
^ mfh
Compound Nana: Internal Ref.: Quantify Trace: Cone, of Standards I
M13 (Nona)
txc Cone. A
Ueer RT Valus:
Osar Peek Factor: Integrata Window Factor:
1.000000, Disabled
1.000000 2.000
Fasi: location
Retention Tina (Bins): Relativa Retention Tina: Tina window- (nine):
5.001, Enabled 0.000, Disabled 0.210
Peak selection: Response
Nearest
Response Type: Response:
External Areas
Calibration Curves
Polynomial Type:
Point of Origin: n t Weighting:
Axis Transformation:
Linear
Exclude 1/X
Nona
Concentration
Units:
Noise
Feak-ta-peak amplitude:
19
Smooth
Smoothing:
Window also (scans): Number of smooths: Smoothing mathod:
D i s a b l e d 3 2
Baselines
Join vallay threshold: Reduce peak tailing: Raise baseline maxima:
30.00 70.00 4.00
Peak Separation
Draw vertical threshold:
Detect shoulders: Shoulder threshold:
10.00 Disabled 10.00
Response Threshold
'
Relative height: Absolute height:
Relative area: Absolute area:
30.00, Enabled 0.00, Disabled
3.00, Enabled ' 0.00, Disabled
OLIWS
(AC Ofhufa,?
Page 3
000710
ltethod Report
Method m * i Lilt modified:
Compound:
P rinted:
CI \MA3SLYNX\xats2.PRO\M*tMJB\OS2599a.ndb ad May 28 07:18:17 1 9 9
4: Pros
Wad K ay 28 0 8 :2 1 :1 9 1999
Compound Mao:
I n t a n a i Raf.: Quantify Trac*: Cane, of Standard*:
Osar RT Valua: Usar P*ak Factor: Zntsgrata Window factor:
Peak Location
Retention Tin* Inina):
Salativ* Retention Tina: Tin* window Inins):
Peak Salactlon:
Raapons
Response Typa: Rasponsa:
Calibration Curves
Polynonial Typa: Paint o Origin: Fit Weighting: Axis Transformation:
concentration
Unita:
Kola
Peak-to-paak enplltude:
Snooth
Smoothing:
window six (scans)t Humber of smoothes Smoothing method:
Baselines
Join valley threshold: Reduce peak tailing: Rais* baseline naxinun:
Peak Saoaration
Draw vertical threahold: Detect shoulders: Shoulder threshold:
Rasoona* Threahold
'
Relative height: Absolute height: Relative area: Absolute area:
pros (Hone] TIC Cone. A 1.000000. Disabled 1.00Q000 2.000
5.281, Enabled 0.000, Disabled 6*2*3 Nearest
External Areas
Linear Exclude 1J X Hone
72
.Disabled 3 2
30.00 70.00 4.00
10.00 Disabled 10.00
SO.00, Enabled 0.00, Disabled 3.00, Enabled * 0.00, Disabled
A h ,,L
L2HW
IA t os/ z U m
Pig* 4
000711
Method R eport
Method tilo: Last modified: Compound :
i r l a tadi
C:\MM3LYNX\rata2.PRO\KathDB\052399a.ndb Mod May 26 07:19:17 19990
5: IS
a d May 26 0 9 :2 1 :1 9 1999
Compound Nan: Internal Rtf.: Quantify Traca: Cone, of Standards:
User nr Vala: Osar Paat Tactor: Integrate Window Factor:
Peak Location
Retention Time (mina): Relative Retention Time: Time Nindow (mins)
Peak Selection:
Response
Response Type: Rasponee:
Calibration Curvea
Polynomial Type: Point of Origin: rit Weighting: Rucia Transformation:
Concentration
Units:
Kola
Peak-to-paak amplitude:
Smooth
Smoothing: Nindow tilt (scans): Number ot smooths: Smoothing method:
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
Peak Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold:
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
is (None) TIC Fixed
1.000000, Disabled 1.000000 2.000
5.029, Enabled 0.000, Disabled 0.304
Nearest
External Areas
.
Linear Exclude
1/X Mon.
93
.Disabled 3 2
30.00 70.00 4.00
10.00 Disabled 10.00
30.00, Enabled 0.00, Disabled 3.00, Enabled 0.00, Disabled
4 / J f t O U W _____ l A c
Pag* s
't
r 000712
^W/An_______________
M asslynx - Sam ple L ist
Sample L is t C:\M ASSLYNX\rats2.PRO\Sam pleDB\060199c.SPL Printed: Tue Jun 0116:19 :1 8 1 9 9 9
F ile Nam e
1 060199020 2 060199021 3 060199022 4 060199023 5 060199024 6 060199025 7 060199026 8 060199027 9 060199028 10 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 18 060199035 17 060199036 18 060199037 19 060199038 20 060199039 21 060199040 22 060199041 23 060199042 24 060199043 25 060199044 26 060199045 27 060199046 28 060199047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 . 060199054 36 060199055 37 060199056 38 060199057 39 060199058 40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 48 060199067 49 060199068 50 06019906S 51 060199070 52 060199071 53 060199072 54 060199073 55 060199074 56 060199075 57 060199076 58 060199077 59 060199078 60 060199079
'
File Text
methanol blank methanol blank tox120, rbl04199-wblk-2 tox120, rbl04199-lblk-2 tox120, rbl04199-5 ppb-2 tox120, rbl04199-10 ppb-2 tox120, rbl04199-25 ppb-2 tox120, rbl04199-50 ppb-2 tox120, rbl04199-100 ppb-2 tox120, rt>l04199-250 ppb-2 tox120, rbl04199-500 ppb-2 tox120,rbl04199-750 ppb-2 tox120, rbl04199-1000 ppb-2 methanol blank to x120,1M -g 1,4/19/99 to x120,2M -g 1,4/19/99 to x120,3M -g 1,4/19/99 to x120,4M -g 1,4/19/99 tox120,5M -g1t 4/19/99 tox120, rbl04199-250 ppb-co-1 methanol blank to x120,6M -g 1,4/19/99 to x120,7M -g 1,4/19/99 to x120,8M -g 1,4/19/99 to x120,9M -g 1,4/19/99 to x120,10M -g 1,4/19/99 tox120, rbl04199-250 p pb-cc-2,5/28/99 methanol blank to x120,11M -g 1,4/19/99 to x120,13M -g 1,4/19/99 to x120,14M -g 1,4/19/99 to x120,15M -g 1,4/19/99 tox120, rbl04199-250 ppb-cc-3 methanol blank tox120,6M -g1, ms, 4/19/99 tox120,6M -g1, msd, 4/19/99 tox120,13M -g1, ms. 4/19/99 tox120,13M -g1, msd, 4/19/99 tox120, rbl04199-250 ppb-cc-4 methanol blank tox120.3M -g1.m s. 4/19/99 tox120,3M -g1, msd. 4/19/99 to x120,41M -g 3.4/19/99, d10 to x120,23M -g 2,4/19/99 to x120,24M -g 2,4/19/99 tox120, rbl04199-250 ppb-cc-5 methanol blank to x120.25M -g 2,4/19/99 to x120,26M -g 2,4/19/99 to x120,27M -g 2,4/19/99 to x120,29M -g 2,4/19/99 to x120,30M -g 2,4/19/99 tox120, rbl04199-250 ppb-cc-6 methanol blank to x120,31M -g 2,4/19/99 to x120,33M -g 3,4/19/99 to x120,34M -g 3,4/19/99 to x120,35M -g 3,4/19/99 to x120,36M -g 3.4/19/99 tox120, rbl04199-250 ppb-cc-1
Page 1
MS File
9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9MIN-PO AA-513-613 9M IN-POAA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9MIN-PO AA-513-613 9M IN-POAA-513-613 9M IN-PO AA-513-613 9MIN-PO AA-513-613 9M IN-POAA-513-613 9M IN-PO AA -513-613 9MIN-PO AA-513-613 9M IN-PO AA-513-613 9MIN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M 1N -P O A A -513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-PO AA-513-613 9M IN -P O M -513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 v 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9 M IN -P O A A -513 -6 1 3 9M IN-POAA-513-613 9M 1N -P O A A -513-613 9M IN-POAA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-POAA-513-613 9M IN-PO AA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613
000713
In let File
9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m in-2 . 9min-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m ln-2
Masslynx Sample List
Sam ple L is t C :\M ASSLYN X\rats2PRO \Sam pleDB \060199C.SPL Printed: Tue Jun 01 16:19:181999
F ile Nam e
61 060199080 62 060199081 63 060199082 64 060199083 65 060199084 66 060199085 67 060199086 68 060199087 69 060199088 70 060199089 71 060199090 72 060199091 73 060199092 74 060199093 75 060199094 76 060199095 77 060199096 78 060199097 79 060199098 80 060199099 81 060199100 82 060199101 83 060199102 84 060199103 85 060199104 86 060199105
H ie Text
methanol blank to x 1 2 0 ,3 7 M -g 3 ,4/19/99 to x 1 2 0 ,3 8 M -g 3 ,4/19/99 to x12 0 ,3 9 M -g 3 ,4/19/99 to x 1 2 0 ,4 0 M -g 3 ,4/19/99 to x 1 2 0 ,41 M -g 3 .4/19/99 tox120, rbl04199-250 ppb-cc-2 methanol blank to x12 0 ,4 2 M -g 3 ,4/19/99 to x 1 2 0 .43M -g 3.4/19/99 to x 1 2 0 .45M -g 3,4/19/99 to x 1 2 0 ,4 6 M -g 3 ,4/19/99 to x 1 2 0 ,4 7 M -g 3 ,4/19/99 tox120, rbl04199-250 ppb-cc-3 methanol blank tox120, rbl04199-wblk-2 tox120, rbl04199-lblk-2 tox120, rbl04199-5 ppb-2 tox120, rbl04199-10ppb-2 tox120, rbl04199-25 ppb-2 tox120, rb!04199-50 ppb-2 tox120, rbl04199-100 ppb-2 to x l2 0 , rbl04199-250 ppb-2 tox120, rbl04199-500 ppb-2 tox120, rbl04199-750 ppb-2 tox120, rb!04199-1000 ppb-2
Page 2
M S F ile
9M IN-PO AA -513-613 9M 1N -P O A A -513-613 9M IN-PO AA-513-613 9M IN-PO AA -513-613 9M IN-PO AA -513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PQ AA-513-613 9M IN-PO AA -513-613 9M IN-PO AA-513-613 9M 1N -P O A A -513-613 9M IN-PO AA-513-613 9M IN-PO AA -513-613 9M IN-PO AA-513-613 9M IN-PO AA -513-813 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M 1N -P O A A -513 -6 1 3 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA -513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613 9M IN-PO AA-513-613
Inlet File
9m in-2 9m in-2 9min-2 9min-2 9min-2 9m in-2 9min-2 9min-2 9m in-2 9m in-2 9m ln-2 9min-2 9m in-2. 9m ln-2 9min-2 9min-2 9min-2 9m in-2 9m in-2 9min-2 9m in-2 9min-2 9m in-2 9min-2 9m in-2 9m in-2
000714
jp ^ > I 6
_________________ __________________
S can n in g Method R aport
Method:
C:\MRSSUrMX\MTS2.9N0\ACSaDB\9KZN-9CM-S13-13 '
Last Modified: Toa Jun 01 14:34:0 19
Prlntad:
tua Jun 01 16:19:2 199
S o lv en t D alay | a ln a ) :
0 .0 0
Tunotion : 1
M M of 1 Maaa air ( 199- >
Istar Channel Balay ( Saca ) : 0.03
Span ( Daltons ) >
0.00
Start Tina ( Mins ) :
.0.00
End Tina ( Mina ) t
9.00
Repasts :
1
Channel Tarant Daughter Dwell
(Sacs) Coll
Energy
(aV)
Cone ( V >
1 ' 413.00 169.00 0.30
20
13
:lunation 2
M M of 1 Maas Vais ciw- )
( )Inter Channal Dalay Seca ( )Span Daltons i )Start Tina 1 Mina i ( ) :End Tine Mins :R e p a s t a
i
Channal Parent Daughter
0 a03 0.00
a0.00
9 00 1 Dm U
. (Sees) Coll Energy (aV)
1 513.00 169.00 0.30
20
.
(Coo V 1
15
lunation : 3 Mm of 1 Mean M r (CSV- )
( ) :Inter Channel Dalay < )Span Daltons i ( ) :Start Tina Mina { ) :End Tina Mins :R a p a a t a
Sacs
Channal Parent Daughtar
0.03 0.00 0.00 0.00 1 Dwell
(Sees)
Coll Energy
(eVl
<Con V 1
1
613.00 169.00
0.30
20
15
Tunction : 4
M I of 1 Mesa 9alr ( 189- >
lutar Channal Dalay ( Seca ) 0.03
Span ( Daltons ) :
0.00
Start Tina ( Mina ) :
0.00
End Tina ( Mina ) : Repaata :
9.00 1
Channal Parant Daughtar Duali (Seca) Coll Energy (eV)
Cona ( V )
1
427.00 60.00
0.30
40
.60
Page X
000715
1
(o ltfe a
Tuning Method Report
Method:
C :\MASSLXNX\RATS2. PRD\ACQUDB\PFOS
Printed:
Tue Jun 01 16:20:59 1999
Page 1
1 SOURCE ( ESP- )
j Capillary Cone Extractor RF Lens
J Source Block Temp. J Desolvation Temp.
]1
. Pressures
1
Analyser Vacuum 1 | Gas Cell
\
|
i i
Set Rdbk 3.00 2.97 15 '-14 50 0.2 150 152 250 253
Rdbk
1.6e-4 5.1e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
Set Rdbk
15.0 1 5 . 0,, 2.0 * . 0.0 56 650
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Ener g y 'Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0
20
2.0 0.0 40 0
650
21
38 20
000716
FACT: DATA REVIEW SUMMARY FORM
f aOste:
Analyst: FACT Study Number Matrix Time Foists
/o k ite )
?TL
Dilutions?
A s'
Analyte 1:
A h A /f'
W a r R ange: / ~ ////// _
LOQ br ds data se t Reevaluated fir differentrange?
a/
\JAnalyte 4:
Acceptable Linear Range: LQQ for dus data set
iluated for different range?
Analyteo: Linear Range:
LOQ for thu data set Re-evaluatedfix dif ferent range?
Analyte 2:
s h ir t
Acceptable T,mear Rang
- /////^
LOQ for this data se t
S S ^ -G /Y S
Re-evaluated for different range?
J Analyte 3:
A lt/?
Acceptable T.nearRange: fj T f
LOQ for ds data set ST*C A A
Re-evaluated fix differentrange?
Analyte 6: \ Acceptable Linear Rail<Mk*
LOQ fix ds data sett^
Rc-cvaluatedfor diffoMotrange? \
Analyte 7: Acceptable linear Range: LOQ for ds data set Re-evaluated for different range?'
000717
\
t t / t O / Coti A q p 3 U T i t l p u * p a 7 3 u * n b : 6 H J 0 * t t W
a> in i> g h o v g uj*-gu w4 gH<oii**0gi<0iiauii0Aiw0iM0tHi0uiaigii0tuifuu*MuiiN<iHiOt0^ a g
oHo0 HIooO*Uoo>(HooAH1oo0HIooOtHot>Ool.0OlgOo_0OaltOo0tOill00OoO0VVOoAIOt00QUo*OW0MOQO)(H00OOlOo0WOO<<oI0OAO0oWlO0OogI4O0O|Oo(OO0i0oO|Ot00o0O|lO00o*IO|OA0Uo|OOt0M0o|OalOH00oO00So00(OOo(0o40O01o*o0J oK(go0*oI(o0-W>I (oo*o04*(ooMo0Wo1o-e004NoNo1oMO0MooVo1JA0 ooOV(o40Ao0oV((o00Ooo0V(o0OooVVo4OOooV0oVPOooUtVoo0o*tvooHoaUoMtoiooooAo1O0*A1oVtoOA^SoVVo0UODIVo1Vo0oVO0OotoAA(oRoO oi0otoonto0otoooo0outo(0oto0o1to*o0o0oVteuUoOoNo0uoVto*Oo-ou0ottooo0ooto0oAoVtooOoAoVo(O0c0oSOP-0-oOt(o0-0v(-oO0o0-t0O-oOtotO-0toO0V-0oOOt-0V-0ooOt-Oo-0tootO00V0-ooOv-000-(SoOOt-00O-ooOi-0V0-oo0Ot-0V0-ooOOv00-too0OtoV0-oovO-001o-ooO0-vV0-oopO0-tIVoo-oOtOo-oVo-OvOoo-oO(t-0ooVoOto0ootOtOo~ovoO-O0o-oOVt-oo0-oOVtoo^DoOt"o(o0otOto~0ovVoOK_o0x"
XKXKXXX XXXX!J*KXX S 5 XXXXXrtXXXXrliH-OTnXO(XOtfXtOiXrOrXtO|XVfo o#* ort rot oct ort oft
o? o
n rt rt OOo
rtitrt ooo XXX
.
.
f t IT ItltftlT
s......s.........X X X
V0r
trOtxrOtxOf xt Orxt OrxtOxr
trOBrOf
xtOrxtOrxtOxn Onx
Orxt
o
oo
___
9oooooo gooooo>.0 0(0K 0i M30 0M 0N <(
H W H NXM 3000
0 MKIMMNM
09 N0 0N 0N 0N 0N 0N 3a*0M 0M 0M 0M 0N 0M
v- **u M M 3**** M
I
M0
0M
0MM0NM0NM30
K IN N
OOO
O ' *> o *
n n n n r t n l n r r tr t n r t r r t r r r r
rTrh
Sxx
km
S
h
x
h
Sf
h
VT
K
h
X
m
>0 0 0 0 0 * 0 0 iX X X X X n n l - ST a r f l
g 0 0 0 0 0 0 9 0M 0N 0N M0 0N 0N O8oM0M0
. . . . __XXX *&SS!ae r t x t r t r t r t r t r
n0J rtrtr -4c
<r 3- i P
T o o o o o o o
(-HX OOOOXOt I
I
(
I
0 0 05 a
^,(1 U N N M N N N N * U U
ftH H A
N
SSxxS
epX
o*
M
0i
W
0i
U
0i
U
0i
U
0T
N
" *1`
*
X M0 N N MV N M* X HVO
*
-
O J (00 H1*0 0NV 0H *H**M
-09
**0* 0*1 0*. 0.
0. *
h
X9
hX I *O 00H
I 0H
I 0H
0"
XV- H * *
3X
*M
-***
Xn i
. -I
0
rtrc rcrcT it
>*>->->-
0 0 0 0 0 0 0 OO0 0 0
vo vo **VO-**to-3X8x1IHt
Ii IIIIII
H JU N W U N mi s i ,
0ooooO l D O K O O t t
W *
N 'S*'S. V*
N10.(0SVNON(0
s
0
VO VOVO0 to to ve
?0 0 * 0 0
N \SSS\
OV0V0 e v o o
sss 7
TM So N* S* S* *S
U o
HV0
HHHH va to vo vo
V0 vo . . . e e
N V .V S S S NNNNfk*
Otovo1010V0u h h h h h \t s \s
UHHHV O NVONOSV00 S0
to V0 V0 V0 vo
vo vo to to vo
>V0 VAM V0
* s
S ill!
u& *
I**** NSSSN U M - *- M o vo vo 0 vo _ sw s. S vo vo vo vo
7vo VOVOVOlO
***0 inm toto to vo O 0S S W ,, *s.(OV0 vo vo V VOVOV0 (O 10
u__*__*__*____*_? f
I I I I I I
NvtHoHvoMvoMvoHo HO i4n Uo Ne Ho Uo teN oH U
t IO
O
ttWoo v(oONtvooVvvooSvvoo
0" 0 O O O
S HM XX
- 8 S S 3 7 MM
dt^ dt A A A M
1 MMNM
3?
>iUI
s? 15
M It
f s si ;
V AM * ft
& i"
ca
e-
!r f . a
o o o
P
05
HAUM H N U U 4H U M H H U 0 O 0 l* 0 M
U U U U U U U l U U t f U .l*inUiVMOUMnOUI4O(0M|*(|l(OUMO(MllU(O(fMlllMO<n NO4 UMO NMO|*O*NO| MO* **O N0O U0O4*O0 0O4 o*U Oo* Hou Ao* Uob o* peMo*Uou*o*4 o*0 o*U oo0 oA to* foN 4oH 0o* Hou 4o*Oo*HoM0onH oo| o*l oNV o**
1*10 tniAtnlnintnininmui
0U 0W vo**
NOvo *Ovo UOK**O100M4
UON
UOA
UOO
OuC JO0*
0* 0uU u001
uu*u*uu*u 0N0 U*U U*0 *0X 0 *0U O0O O4U UMO
10 (1* u * u u u u u u u * u * u
0u0
VpOvMo O VO
HOU 40 nUOuUOoNOu UO4 MO0 *NO u*O iNUNUO*40u **i
i* a s
UM** *(*IP *N U Mn h *t*04UNOeO
NU0U HOV0UI00 HI tJ M in * U * O V 0 M O U H
luUno4UunPot0h0uNm*m4n40o(<
i a * H to M m ^ M vo i0 (* N U i4 * a K t* u
o a ve a to to u* a w est
O 4U M H M U 4 VIU00 * u n U 0 0 N N 4 0 O 4 UO o o n 0 O O O o v 0M(n * H 0OO 0UON*N
4N4IAN*mH- uwoienun it
MMOWOV0
MOvOtOMOMMMnMnOOMMWOOOU A n A U t t S e U 0 g u O e * S A A N O U * 4 l0* A M O r ` A O * * 0
*M *W U *n A U M N U > U U H M H 0 M4O H H U AA 4 U * 4 * A U * n 4 A 4 4 4 U N U e A O U A M 4 l0 U *
ggggggggggggggggggggggggig$
M VOM An M M W M U M M 0 4 O 4 A A NV0 N * U * H 4 H H O * 0 OOVS
uuMotovooMMmuvo U(H0 4 N U 0 B * 0 A
ssm sssm s
N to * 0 00 00
gg
JI0 W O U 0 0 A *
HM
h
OMO U N A U A O
4W0 4 U M N A 0 * O 0 U U 4 OO
0M u 0 0 M 00 01 U 10
? g
UMMH U O O N 0N U HH U W H O U *U 4 *(0 I0 N V
* U 0M AABH *N H O A H 0 |0U 0 A M W * O H 0 4 U U A B U N 4N U 0 HOW4 A A A H A M O A O
gggggggg%S$gg
r
.-
o00* cr *
T
AI l0 * H M MM 4MOON0
I HANHn O0V00U4U0
I
Q u a n tify Compound Summary l U p o r t to*120, 1*3# r t l
ampio tlat: C:\MMSLYMX\rits2.mO\SamplaCa\0CO199e
Laat modlfiod: Tuo Jun 01 16:18:01 i m
Hothod :
Ci \MAA5LYHX\rats2.9N0\HmthD\040199e
Laat modlfiod: Nod Jun 02 06:48:03 199
Job Codo:
frintod:
Nod Jun 02 07:20:4? 1999
Compound 2: HS13
Hama 1 040199020
2 040199021
3 04Q199Q22 4 040199023
3 040199024 4 040199Q25 7 040199024 8 040199027
$ 040199028 10 040199029 u 040199030 12 040199031 13 040199032 14 040199Q33
IS 040199034 14 040199035 17 040199034 18 040199037
19 040199038
20 040199039 21 040199040 22 040199Q41 23 040199042
24 040199043 2S 060199044
24 040199045 27 040199044 28 040199047
29 040199044 30 060199049 31 040199Q5Q 32 040199051 33 060199052 34 060199053 35 040199054 34 040199QS5 37 040199054 38 040199057 39 040199054 40 060199059
41 040199060
42 040199061 43 040199042 44 040199043 45 040199044 44 040199065 47 040199064 48 040199047
49 040199044
50 040199049 51 060199070 52 060199071
S3 060199072 54 060199073 55 060199074 54 060199075 57 060199076 58 060199077
59 060199078 40 060199079 41 060199080 42 060199081 43 060199082 44 060199083
45 060199084 44 060199085 47 060199086 48 060199087
89 060199088 70 060199089 71 060199090 72 060199091 73 060199092 74 040199093 75 060199094 74 060199095 77 060199094 78 060199097 79 060199099' 80 060199099 81 060199100 82 080199101 83 060199102 84 060199103 85 060199104 84 060199105
Taxt nathanol blank
nathanol blank tox!20, rbl04199-nblk-2
toxllO, rbl94199-lblk-2 toxl20, rbl04199-S ppb-2 (0X129, rb!04199-10 ppb-2 toxUO, rbl04199-2S ppb-2 tOX120, rbl04199-S0 ppb-2 toxl20, rbl0419-100 ppb-2 toxl20, rb!04199-2S0 ppb-2
(OX120, rbl04199-500 ppb-2 (OX120, rbl04199-OSO ppb-2 (OX120. xbl04199-- 1000 ppb-2
M(hanol blank (OX120, IH-gl, 4/19/99 (OX120. 2H-gl, 4/19/99 (OX120, 3M-gl, 4/19/99 (OX120, 4H-91, 4/19/99 (OX120, SK-ol, 4/19/99 (OX120, rbl04199-2S0 ppb-ce-1
m(hanol blank (OX120, CK-fl, 4/19/99 (OX120, TK-ol, 4/19/99 (OX120, OM-gl, 4/19/99 (OX120, SK-gl. 4/19/99
(OX120, lOK-gl, 4/19/99 (OX120, zbl04199-2S0 ppb-ec-2, rn.th.nol blank
(0X120, llH-gl, 4/19/99 (OX120, 13M-gl, 4/19/99 (OX120, 14M-91, 4/19/99 (OX120, 1SK-ol, 4/19/99 (OX120, rbl04199-250 ppb-cc-3 xa(hanol blank toxl20, CM-gl, , 4/19/99 (OX120, K-gl, msd, 4/19/99 toxl20. 13H-gl, na, 4/19/99 (OX120, 13H-gl, nad, 4/19/99 (OX120, rbl04199-250 ppb-cc-4
nathanol blank (OX120, 3M-gl, ns, 4/19/99 (OX120, 3K-gi, nod, 4/19/99 (OX120, 41M-g3, 4/19/99. dlO (OX120, 23K-g2, 4/19/99 (OX120, 24M-g2, 4/19/99 (OX120, cbl04199-- 230 ppb-ce-5 nathanol blank (OX120, 25M-g2, 4/19/99 (OX120, 2<M-g2, 4/19/99 (OX120, 27M-g2. 4/19/99 (oxl20i 29M-g2, 4/19/99 (0x120, 30M-g2, 4/19/99 (OX120, tbl04199-2SO ppb-cc-4 nathanol blank toxlOO, 31M-g2, 4/19/99 boxl20, 33M-g3, 4/19/99 (0x120, 34M-gl, 4/19/99 (OX120, 3SH-g3, 4/19/99 (oxUO, 3<M-gl, 4/19/99 (oxl20, kbl04199-230 ppb-cc-1
na(hanol blank (oxUO, 3m-gl, 4/19/99 (0x120, lK-gl, 4/19/99 (0x120, 39K-g3, 4/19/99 (OX120, 40M-g3, 4/19/99 (OX120, 41M-g3, 4/19/99 (0x120, cbl04199-23O ppb-ee-2 nathanol blank (OX120, 42M-gl, 4/19/99 (OX120, 43K-g3, 4/19/99 (OX120, 43H-gl, 4/19/99 (0x120, 4SM-g3, 4/19/99 (OX120, 47M-g3, 4/19/99 toxl20, rbl04199-230 ppb-cc-1
nathanol blank (OX120, xbl04199-wblk-2 toxl20, rbl04199-lblk-2 (OX120, rblO.lSS-- 3 ppb-2 (0X120, rbl04199-10 ppb-2 toxl20. tbl04199-23 ppb-2 (OX120, rb!04199-30 ppb-2 (0x120, rbl04199-100 ppb-2 (0x120, rbl04199-2S0 ppb-2 tox!20, rbl04199-300 ppb-2 COX120, rbl04199-130 ppb-2 tox!20. rb!04199-1000 ppb-2
S/20/99
Amnlla 042491: q u a n tia to d and p rin to d b y b o j 4 /0 2 /9 9
a 2
*> Analyta
td Cono
Analyta
Analyta
Analyta
Standard
5.5
Standard
11.10
Standard
27.10
Standard
SS.SO
Standard
111.00
Standard
270.00
Standard
55.00
Standard
033.00
Standard
1111.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 270.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
OC Analyta
270.00
Analyta
Analyta
Analyta
Analyta
QC Analyta
271.00
Analyta
Analyta
Analyta
Analyta
QC 270.00
Analyta .
Analyta
Analyta
Analyta
Analyta
Analyta
QC 27.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 270.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 278.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 270.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 270.00
Analyta
Analyta
Analyta
Standard
S.S
Standard
11.10
Standard
27.00
Standard
35.(0
. Standard
111.00
Standard
271.00
Standard
55.00
Standard
133.00
Standard
1111.00
ST S.310
S.484 3.477 3.43 3.470 3.437 3.457
3.481
5.47
5.413
3.474 5.4 5.477 5.509 3.4(1
3.473 S.S03 3.470 3.479 3.482 3.492
3.491 5.4(0 3.473 3.430 3.4 3.487
3.474 3.305 3.475 3.490 5.47 5.474
3.412 5.300 3.490 3.474 5.4(3 5.4
3.47 3.479 5.49 5.4(0 5.4(3 5.493
5.514
5.437 5.49 5.492 3.494 3.472 3.473
At m 6
Cone, fina %0v 33.86 bb
4 38.43 bb 17 155.79 bb
33 340.82 KK 58 626.52 bb 78 650.34 KM 105 1156.36 KK
-31 40
23
13 2 4
22 215.92 MM
-22
28 286.55 MK
3
26 259.87 M M
-7
24 238.96 bb
29 292.29 M M
22 215.83 M K 26 265.21 MM 29 295.89 MK
6
23 224.10 MM 23 225.97 bb 10 78.75 bb 115 1272.23 M K 147 1632.39 MM 27 271.64 MM
-2
99 1083.93 M M
123 1360.34 MM 191 2126.03 M M 118 1302.00 MM
75 920.19 MM 25 250.88 bb
,7 -10
87 956.99 MK
102 1127.3? M M ` 135 1492.73 H i
92 1013.14 MM 92 1010.59 Ml 26 260.57 bb
-6
100 1104.83 Ml
75 819.95 Ml 109 1201.84 MM
96 1051.95 W 86 942.87 MK 29 294.92 bb
6
78 851.36 MK 97 1067.95 MM 57 610.50 Mf 65 707.74 bb 47 496.73 bb
30 307.89 bb
11
4 14.64 MK
6 40.05 MM 10 1.11 bb 25 254.54 MM 46 490.00 MM 78 854.07 MM 91 995.71 M M
32
-29 -27
-6 -12
3 -10
000719
SS2SS2Sii3i!iCrfiiSS5S3SSSSiSSSSi52SSSSSil:fiSCi:i:SSSt5SSS:!SiS!gS5iiS!tiS!5iiSS = iJS:S5:UEiS--ui*.u--
22222222222222222222SSS22SSS22SSf2S2SS2S2f22SSS2S2S2SSSS222SS2S2S22gg|25|!2222gg| T S ooooqqooS ooooooooooooooooooooooeoooooooooooooooog
iaaa*a
...........Hoooooooooooooooo^eooooooodooooodooovo
ot oAoUoMo HM
>etfio0 *i
suwi*ADUi N0
WHUOM0A
<a
aJiniuait *a
iwaMAH(Oi t(
tt 4**lJ A'>J('i4*^'UiM'4H>4o-i> <o'aJ*n AJAAO iUoit
OSO
luOOMOiHOO
OOI
Oe
o Ao"OUfUOtUOUUOO*OOUOONUOH>OOUtOUSiOV*Og*aO*UO*lO**OU*MO*HO*OO* WOa U*OVJO)AOUO
UO WO0_..WU...O_.0.W_N.._..O._UH0.._.._U0o__N<_0_aN_0_N_0_wN_a0_M_i_0_iM_0a_Nx0Muv0NoM0Kt
a0 NO .
!ei SS e rtrtrtE S aoooooog m i Sa*
OBOI
KXX)
Io o o o o o CX X X X X X OOOOOO
t t #r r t t r t r t
rtiti
OOOOOO O O O O O O
OOP
M X X X X X - X X X X X X rt X X X
hhhmhh
M M M M M M ?... 9 O 0-0 O O O g.oio o o o o :
fftittffrtrt
OOO o o o o p * X X X t* X X X X X
1 M M M N N X
n
a
rt
o XX m
t rr tr r t rt
ooooo XX XX XX XX XX s xmnwm
ffr tttffr tl rtrtrtitrt
XnOX XnOX Xm-t<I>0H"xNH0x"Nr*"fIt0H'Nx'0H"Nx0HM"x0HMx" 0HxN"
B.'"
it
or t or t ? o o ? S
X X X X X X rt MMNMNM
>g O O O O O O 9 O O O O O O --------------->009o O *O *O O* O
r t r t r t rt c
" o o oc X X rtX> V
MNMWNM i Nf o o o o o o 9 OC
O' "
tf*rtffr*r*rfnrfW I I
O O O O O O O p i I X K X X X X X X rt rt l
SrT IMMNMMNMNt H
>0 0 s0 0 0 *0 0 0 9o 9O t*
* *-
m
HAAUUO
N u u UUU
H UMMMM
i oooooooooooa s s s s s s a : s s s -- **-------O oxoi I IIOTAA4I OAAi S E i S K a S . 0 1 1 1 1 7 1 0 1
tm
* *irHr * H
<HHHHHHV^,
WUUUW
9 XAOOOO (THUUWUW
l OOO w
Ir 1*n0Au u%Uv
s c iis iia a s tot I 0 0 9 > A 4 4 HH KrtKiNU*
0 *AA l
____- O O O X
ithhX
HA` HK)
H
4K`
H<`
9
O O......H......H......H......H.... 0
0 * * *
o*
"
?I 8 f <l*iltio *f* * * * * > s. s. sT * * * * * ? * 1 1 e e *t* 3
OOO w
I I l 1 I I I I I (
uo> ae
MH OO
UlMHtfH O 4* 0 _ O* S'
a Hi-
& INN
M S\SV \
OOOOOO N V N SS OOOOO OOOOO
N SW SS
oI DoHoHoHoMoH
N \> \\ *0 O O O O O *0 O O O O O
N SN S
n\SV 000
N S N 'V N. S
Wo oHoHoH oH oH
N SSSN 00000 OOOOO
eb
N N SV i*
O Os O\ Os * s*
EE
a * 0. o*
M SSSS ItHHHH OOOOO
SSSN V Oo o o ^ow
M'.. U>H
HH
O O
H
tOt
o ia ssss
*>.0000
"> 0 0 0 0
5 . . ?TN N N I MNNW
T 8
aoo
HO
00000000 To ? ooooo
NrtltNrtplH
O O O O 0_ v U
Sx S
-2
2H 9
f**- a
4 1
0 0
vi
f l s s S S S S S sS s f& s S * H S S S S sS fsS S H S 's s i' S S f f * s S f p
*
aaa& o.ftA P.a
sssss?
ss****
(RMtlMUHtttt "if ? ? ? ? p ? ?
9 S 9 U*-*-*-!
f r a | ? < s -5
Laaanaaa
**
M>4 m
M0<4
N*a4
M4
o0 o0 o0 o0 o0 0 0 h0 u*
00
0O
00
OO
N<B4 M4 *4O4 144 N4O OO OO OO OO OO
H HhX
UUa
Wu 4NHHhIt
Af
M4
HH
U
OO OO DO OO OOXOMOH OU Ot
M
E
E
i
uuwuuui a 4 4 0 m 4 iOOBN
mia U*400
O
O (X (X o o o o o o O O O O O O O O O O O
O
0e
*O44
V0i Ut
0Hu 4*O 4O 04O X4O 44 0H 0*<04-444O<*44 OO4 OO4 OX4 OX0 OH4 UO4 OO4 O44
O144
O OOOOOO OOO
8444 O44 H44 A40 U40 4* 4A0 *O0 4H40U4*
rtNIf Al NNIHM t
4WMH M4UHM 0 M H J O O * -4*0
g53535
MOlI* *I OHl UinI
O OM
HM u0 aU tm*
5?
M*I*
/"
Mu
1U4t X
t*** 1U4S OW MUtH* U teoto*o NH N14 M14MM MO H4 HO O>
NO
1H4.
OH HO U0*H4 OH * 0 *A*1 4
ir 1
M N
X 4 0
** M H H H <4 N M H < 4 H
UNU HNH A
1NO41*O4 1Ua4UH14MUMtoMt uAM
N O
M4
W(4
*OO*AX IAAUM HM * t0-0H( 4
p
U
*N 0
1O4 UaH Au 4a iU N U0 *h 0u MO M* *0 04 0* 00 0* 1t*4
O4
*
UW *M 4400**4 *0 *U*A(A4O0
a
3
3 ? ? 353553 555 55 55555 3
5
5.
35553 g|5
3 51
1
sO
O
14
*O14
H*1
w4
*1-* H1 O .O
1 H1 >14 *4A*0O4 04**H4 M*
Q u a n tify Compound S u m a ry K a p e rt to r 120, Ql-3 , r t l
Sampl. U a t : e : \ M K M L Y M X \ n t e 2 . M O \ S M pl a O B \ O M 1 3 9 a
taat nodlfled: Tue Jun 01 1*:1S:01 1*9
Method:
C:\MA3SLTWX\rata2.PBO\M.thOO\OS01Mo
Last modified: W e d Jun 02 OS: 4*: 03 199*
Job Cede:
Printed:
Wed Jun 02 07:20:47 1999
Compound 4: IS
Ha " t 060199020
2 060199021 3 060199022 4 060199023 S 060199024 6 060199025 7 060199026 9 060199027 9 060199028 10 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 15 060199035 17 060199036 19 060199037 19 060199038 20 060199039 21 060199040 22 060199041 23 060199042 24 060199043 25 060199044 . 26 060199045 27 060199046 28 060199047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 060199054 35 060199Q5S 37 060199056 38 060199057 39 060199058 40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 49 060199067 49 06Q199Q68 50 060199069 51 060199070 52 060199071 S3 060199072 54 060199073 55 060199074 56 060199075 57 060199076 56 060199077 59 060199078 60 060199079 61 060199080 62 060199081 63 060199082 64 060199083 65 060199084 66 Q60199085 67 060199086 68 060199087 69 060199088 70 060199099 71 060199090 72 060199091 73 060199092 74 060199093 75 060199094 76 060199095 77 060199096 71 060199097 79 060199498 60 060199099 61 060199100 82 060199101 63 060199102 64 060199103 65 060199104 86 060199105
Sappiali, t Text ethanol blank ethanol blank
toxl20, rbl04199-eblk-2
COX120, rbl04199-lblk-2
toxl20, rbl04199-S ppb-2 toxl20, rbl04199*10 ppb-2
toxl20, rblQ4199-2S ppb-2
toxl20, rbl04199-50 ppb-2 toxl20, rbl04199-100 ppb-2
toxl20, rb!04199-2S0 ppb-2 t0X120, cbl04199-300 ppb-2
toxlZO, rbl04199-750 ppb-2
toxl20, rbl04199-1000 ppb-2 ethanol blank t0X120, lM-gl. 4/19/99 toxl20, 2M - 9 1 . 4/19/99
toxl20, 3H-gl, 4/19/99 . toxl20, 4M - 9 1 , 4/19/99
t o x H O , SM-gl, 4/19/99
toxl20, rbl44199-2S0 ppb-ce-1 ethanol blank
toxl20, SM-gl, 4/19/99 toxl20, 7M-gl, 4/19/99 toxl20, (M-gl, 4/19/99 toxl20, 9M-gl, 4/19/99
tOX120, 10M-gl, 4/19/99
toxl20, rbl04199-2S0 ppb-cc-2, ethanol blank toxl2Q, llH-gl, 4/19/99
toxl20, 13M-gl, 4/19/99 toxl20, 14M-gl, 4/19/99
' toxl20, 1SM-gl, 4/19/99
toxl20, rblQ4199-250 ppb-ce-3 ethanol blank
toxl20, 6M-gl, a, 4/19/99 tOX120, SM-gl, Bad, 4/19/99 toxl20, 13M-gl, as. 4/19/99 toxl20, 13M-gl, Bad, 4/19/99 toxl20, rbl04199-290 ppb-cc-4
ethanol blank toxl20, 3K-gl, xa, 4/19/99 toxl20, 3M-gl, Bad, 4/19/99 toxl20, 41M-g3, 4/19/99, dlO COX120, 23M-g2, 4/19/99 COX120, 24M-g2, 4/19/99
toxl20, rbl04199-250 ppb-cc-S ethanol blank COX120, 25M-g2, 4/19/99
toxl20, 2SM-g2, 4/19/99 COX120, 27M-92, 4/19/99 COX120, 29M-g2. 4/19/99 toxl20, 30M-g2, 4/19/99 COX120, rbl04199-250 ppb-cc-S
ethanol blank toxl20, 31M-g2, 4/19/99 tOxl20, 33M-g3, 4/19/99 toxl20. 34M-g3, 4/19/99 toxl20, 3SM-gl, 4/19/99 toxl20. 3SM-g3, 4/19/99
toxl20, rbl04l99-2S0 ppb-ee-1 ethanol blank toxl20, 37M-g3, 4/19/99
toxl20, 3SM-g3, 4/19/99 toxl20, 39M-g3, 4/19/99 toxl20, 40M-g3, 4/19/99
toxl20, 4 W -g3, 4/19/99
toxl20, rbl04199-230 ppb-cc-2 ethanol blank
toxl20, 42M-g3, 4/19/99 toxl20, 43M-g3, 4/19/99 toxl20, 45H-g3, 4/19/99 toxl20, 46M-g3, 4/19/99
tox!20, 47K-g3, 4/19/99 toxl20, rbl04199-2S0 ppb-ce-3 ethanol blank
toxl20, rbl04199-wblk-2 toxl20. rbl04199-lblk-2 tox!20, rb!04199-3 ppb-2 toxllO. xbl04199-10 ppb-2
t0x120, rbl04l99-2S ppb-2 COX120, rb!04199-50 ppb-2 toxlZO, rbl04199-100 ppb-2
toxl20, rb!04199-2S0 ppb-2 toxl2Q, rbl04199-S00 ppb-2 t0x120, rbl04199-730 ppb-2 toxl20, rbl04199-1000 ppb-2
S/2S/99
Amelia 0S249S: q u a n ti a te d and p r in t e d by h o i S/02/99
Page t
Type Analyte Analyte Analyte Analyte Standard standard Standard Standard Standard Standard Standard 'Standard Standard Analyto Analyta Analyte Analyte Analyte Anelyte QC Anelyte Anelyte Anelyte Analyte Anelyte Analyta OC Analyta Analyte Analyta Analyte Analyte QC Anelyte Analyte Analyte Anelyte Analyte QC
Analyta Analyta Analyte Anelyte Analyte Analyte QC Anelyte
Anelyte Anelyte Anelyte Analyte Anelyte QC Analyta Analyte Anelyte Anelyte Analyta Analyta
QC Analyta
Analyte Analyte
Anelyte Anelyte Analyte QC Analyte Anelyte Anelyte Analyte Analyte Anelyte QC
Analyte Anelyte Anelyte Standard Standard Standard Standard Standard Standard
Standard Standard Standard
ltd Cone
**
1.00 1.00 1.00 1.00
1 e00 1.00 1.00 leOO 1.00
S.0S1 S. 0S1
5.024 S.031 S.01*
5.032 5.021 5.027 9.014 5.02S 5.003
. 1.00
5.024 5.01S S.04S 5.02 5.010 5.024
1.00
5.023 5.014 5.024 5.004 5.005 5.((21
1.00
5.02* 5.014
5.023
5.023 5.027
1.00
5.021
5.033 5.023 5.030 5.024
' 5.01C
5.025 5.022 5.023 5.025 1.00 5.033
1.00
5. QIC 5.024 5.01S
4.55 5.013
5.015
1.00
5.042 5.004
5.019 5.012
5.019 5.010
1.00
5.025 5.021
5.042
5.019 5.005
5.014
1.00
5.022 5.049 5.01S S.02S 5.033
5.015
XeOQ 1.00 1.00
1.00 1.00
1.00
1.00 1.00
1.Q0
5.050 5.034 5.039 5.034 5.037
5.023 4.997
5.01S 5.01C 5.020 4.999
Ar*
Cone, Flag* lDv
862 1055 1051 1057
1119 909
1047
906 907 899 972
0.92 bb
1.13 bb 1.12 bb 1.13 bb 1.20 bb 0.97 bb
1.12 bb 0.97 bb 0.97 bb 0.96 bb
1.04 bb
779 0.83 bb 73 0.93 bb
172 0.93 bb 909 0.97 bb 764 0.92 bb 870 0.93 bb
691 0.74 bb 730 0.78 bb 809 0.87 bb 700 0.75 bb 970 0.93 bb 953 1.02 b b
702 0.7S bb 684 0.73 bb 783 0.84 bb 667 0.71 bb 963 1.03 bb
768 0.82 bb
763 0.82 bb 696 0.74 bb 810 0.87 bb 948 1.01 bb
36 0.99 bb 77 0.94 bb
93 0.10 bb 466 O.SO bb 555 0.59 bb 886 0.95 bb
541
523 1219
779
536 9$
0.58 bb 0.54 bb 1.30 MM 0.93 bb 0.57 bb
0.96 bb
591 985 509 . 457 546 814 . V.
517 483 630 466 471
940
0.63 bb 1.05 bb 0.54 bb 0.49 bb 0.59 bb 0.97 bb
Q.SS bb 0.S2 bb 0.67 bb O.SO bb 0.50 bb 1.01 bb
659 0.71 bb 566 0.61 bb 535 0.57 bb 615 0.66 bb 534 0.57 bb 836 0.69 bb
913 >23 923 974 1061
108 978
843
7S8 764
850
0.99 bb
0.99 bb 0.99 bb 1.Q4 bb
1.14 bb 0.96 bb 1.05 bb
Q.9Q bb 0.81 MM .0.82 bb
0.91 MM
-8 13 12 13 20 -3 12 -3 -3 -4 4
-17 -7 -7 -3
-18 . -7
-24 -22 -13 -25
-7 2
-23 -27 -16 -29
3
-18 -18 -26 -13
1
-11 -6
-90 -30 -41
-5
-42 -44
30 -17 -43
-4
-37 5
46 -51 -42 -13
-45 -49 -33 -50 -SO
1
-29 -39. -43 -34 -43 -11
-2 -1 -1
4 14 -14
5 -10 -19 -18
-9
000721
b k \ Ch fat 4Ao>
Quantify Calibration Raport to*120, fl1-3, rtl
Calibration) Ci\MMOUfWC\rata2.R0\Curara\OOO199a
U o t nodiflad: Wad Jan 02 07il(>0( 18 11
Prlntad:
Wad Jun 02 07:20:91 1118
AaMlla 002490: q u a n tia ta d and p r ln t a d b y h o j 0/02/99
000722
Q uantify C alib ratio n Raport to*120, (1 -3 , r t i
Calibrations C:\MJftanoc\rataZ.9IIO\Curvooa\090199a
Laat aodlflod! (ad Jun 03 07:19:09 1*9
Prlntad:
(ad Jun 03 07:30:31 19*9
9aja 3
alla 093499: piantlatad and p rln to d by ho) 9/03/99
000723
Q uantify C alib ratio n apace to*120, 1 -3 , r t l
Calibration: C:\MM3UNX\rsta3.M\CtfrvaDa\0019fa
Laat laodlflad: W a d Jun 03 07:10:0 1*9
rlntad:
ad dun 03 07:30:31 If
Pia* i
Aamlia 0C2498: q u a n tla ta d and p rln ta d b y b o ) /0 3 /f f
000724
SOUP 020199: FACT Equipm ent L og 99014
Q uattro II, #6414E
PAGE
DATE: 06/08/99
ANALYST: drb
STUDY NUMBER:TQX-120 M ATRIX:R at Liver DESCRIPTION: PQ AAanaIog_C_hcsk FACT M ethod: ETS-8-5.0 ,
Injection volume: 10 uL
Cycle time: 9 min. E lectrode:.Z -SpPy-------
M obile P hase:
(A12.0 mM NH4CH3CQ2
(B ) M eQB--------
TN-A- o r stndrd num ber (A) 99012-10-9________
(B):TK-A:254fiL
Prepared (date/by):
(A1 06/04/99 drb ______
(B), Q6/03/29_dijb_
Expires:
(At 10/99_______________
( B ) _ lf lS L -----
Store at room tem p? @ or N
Other:----------- :--------------- ----------
G u ard colum n: N one _______________
P art number: na_________
L ot number: na
A nalytical colum n: Keystone B etasil
-----------------------------------------
Size: 2 X SO mm_______________ _ Type: C18____________ Sphere size:J jjs a ----------
Part number: 0S5-701-2________ _ Lot number: P7LQ4
Serial num ber:Q481175Z
A nalytical p aram eters:
Drying gas fIVhrl: 350
ESI nebulizing gas (L /h r)J5 ____ Sheath gas (L/hr)_0
LC Flow rate: 300 uL/m in. B ack pressure: 55 b ar
''
H PLC solvent g rad ien t: 9m in-2
lim s im iik L
l io.oo
2 ) 1 0 0 ....
314.50
4 1 6.50
51 7.00
%B 40.0 40.0
.210
21Q_
40.0_
000725
M asslynx Sam ple List
Sample List: C:\MASSLYNX\soup-rats.pro\SampleDB\060899a.SPL Printed: Tue Jun 0815:56:34 1999
Page 1
File Nam e
1 060899001 2 060899002 3 060899003 4. 060899004 5 060899005 6 060899006 7 060899007 8 060899008 9 060899009
F ile T ext
M S F ile In le t File B ottle
MeOH Blank Tox-120, RBL04199-UverBlk-1,4 /1 9 /! Tox-120, RBL04199-10 ppb - 1 ,4/19/ Tox-120, RBL04199-100 ppb -1 ,4 /1 9 , Tox-120, RBL04199-1000 ppb -1 ,4 /1 : MeOH Blank TO X -120,19M G 2 MeOH Blank T O X -120,35M G 3
POAA-51: POAA-51! POAA-51: POAA-51: POAA-51: POAA-51: POAA-51: POAA-51: POAA-51:
9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m In-2 9m in-2
91 1 2 3 4 91 5 92 6
4 V olum e S a m p le T C one A
10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Analyte 10 Analyte 10 Analyte 10 Analyte
10 100 1000
000726
000727
a c u m i n a Katfced Sapore *
Maehodi
c>\nsiumx\poDV-iiua.M0vacaasa\aAk-si3-<ii'
U i t Modifiadt t u A m 01 ll:55i54 U S *
printad i
Xu* A n 0* 15t00i44 I S M
Solvent Stly ( alas ) j
0.00
Puaetioa : 1
M M of 1 M u i n i r < Uff- J
Zatar Channel Delay ( Sacs ) i Spaa ( Daltona ) t Start Tina { Mina ) i
Sad Time { Mina ) :
Sapaata t Channal Paraat Daughter
0.03 0.00 0.00
9.00
1 Dwell
(Seen)
Coll
Snargy
(aV)
Corn ( V )
1
413.00 109.00
0.25
20
IS
function s 2
m m of 1 Maas vaia ( Uff- )
Zntar Channal Dalay ( Saca ) <
Span ( Daltons ) > Start Tina ( Mina ) i Sad Tiaa ( Mina ) s Sapaata i
Channal Parant Daughter
0.03
0 .0 0 0.00 9.00 1 Duali (Saca) Coll Snargy (aV)
Cona ( V )
1
513.00 169.00
0 .2 S
20
15
function 3
M M of 1 M a i pair ( BSP- )
Zatar Channal Dalay ( Saca ) Spaa ( Daltoaa ) t Start ?Jj m ( M i n a ) i Snd Tiaa ( Mina ) i
Sapaata * Channal Paraat Daughtar
0.03
0.00 0.00 9.00
1 Dwell
SKI)
Coll Snargy
t.V)
Cena t V )
1
613.00 169.00
0.25
20
IS
Puaetioa t 4
M M of 1 M u Pair ( SSP- )
Zntar Chaanal Dalay ( Saca ) s Spaa ( Daltoaa ) i Start Tiaa ( Mina ) i
Snd Tiaa { Kina ) t Sapaata t
Channal Parana Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Sac.)
Cell Snargy
(V)
Cona ( V. )
1
363.00 119.00
0.20
20
15
yuaotioa i 5
M M of 1 Naaa Pair C mar- )
Zatar Channal Dalay ( Saca ) t Span ( Daltoaa ) i
Start. Tiaa ( Mina ) i
Snd Tina { Mina ) i Sapaata *
Channal Paraat Daughtar
0.03
0.00 0.00 9.00 1 Dwell (Sac.) Coll Snargy t.V)
Cona t V >
1
463.00 169.00
0.20
20
15
Function f _ m o l of 1 Kuril Pair ( K4F- >
Zntar Channal Dalay { Sk i ) i
Spaa ( Daltoaa ) i Start Tiaa ( Mina ) Snd Tina ( Mina X t Sapaata *
Channal Paraat Daughtar
0.03
0.00 0.00 9.00 1 Dwell (Sac.) Coll Snargy (V)
Coaa < V )
1 563.00 149.00 . 0.20
20
15
Function 1 7
uni of 1 H u . Pair ( U P - )
Zatar Channal Dalay ( Saca ) i Spaa ( Daltoaa ) i
Start Tina ( Mina ) t Bad Tina ( Mina ) *
Sapaata t Channal Paraat Daughtar
0.03
0.00 0.00
9.00 1 Dwell (Sac.) Coll Snargy t.V)
Coaa < V )
1
663.00 169.00
0.20
20
15
5 S o & fa
Page 1
000728
Tuning Method Report
Method:
C :\MASSLYNX\SOUP-RATS. PRO\ACQUDB\PFOS
Printed:
Tue Jun 08 16:43:16 1999
o fo /o e /f
Page 1
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 60
5 0.2 150 250
3.01 -60 -7
153 252
Pressures
Analyser Vacuimi Gas Cell
Rdbk
2.2e-4 4.9e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
\
MS2
Lto Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
14.0 14.0 2.0 0.0 52 650
Set Rdbk
15.0 15.0 45 2.0
0.0 40 0 650
43
38 43
000729
AM ELIA062498: FACT Equipm ent Log 99016
Q uattro n,# 6 4 4 8
PAGE
DATE: 06/09/99
ANALYST: sah/mee
STUDY NUMBER Tox-120, M ATRIX: ra t liver DESCRIPTION: IP
FACT M ethod: cts-M -5.0________
Injection volume,lOuL
Cycle tim e: 13.5 min.
Electrode: Z-Spray
M obile Phase:
(A12.Q m M NH4CH3CQ2
(B1 MeOH
TN-A- or stndrd num ber (A ) 99012-10-6_________
(B) TN-A-2647
Prepared (date/by):
(A) 5/21/99 ias__________
(B1 6/7/99 sah
Expires:
(A) 11/99_______________
(B1 12/99________
Store at ropm tem pi [7] or N
O ther
G u ard colum n: None___________________
Part num ber na '
L ot number: na
A nalytical colum n: keystone bctasil_____________
Size: 2 x 100mm ""
Type: cl 8
Part num ber 105-705-2______ L ot num ber
p7h!2
Sphere size: 5 um /
Serial num ber 068640b
A nalytical param eters:
Drying gas (I/h r): 300
ESI nebulizing gas fL/hr) 15
LC Flow rate: 300 uL/m in. Back pressure: 80 b ar
Sheath gas (L/br)_0
H PLC solvent g radient: p fb sl
Tim e (m in.) IV 0.00________
%B 5___
______________
3 8.5_________ 51 11.0________ 61 12.0 . 71 13.5________
1__
90 90 5___ end
000730
M usU k
M asslynx - Sam ple List
S A K iA f c f _____:
Sample U s t C:\M ASSLYNX\am elja-rats.PRO\Sam pleDB\060999a.SPL Printed: W ed Jun 09 15:01:191999
File Nam e
F ile Text
1 060999001
methanol blank
2 060999002
methanol blank
3 060999003
tox120, rb!02229-H2Oblk-1
4 060999004
tox120, rbl02229-Llverblk-1
5 060999005
tox120, rbl02229-5ppb-1
6 060999006
tox120, rbl02229-10ppb-1
7 060999007
tox120, rbl02229-25ppb-1
8 060999008
tox120, rbl02229-50ppb-1
9 060999009
tox120, rbl02229-100ppb-1
10 060999010
tox120, rbl02229-250ppb-1
11 060999011
tox120, rbl02229-500ppb-1
12 060999012
tox120, rbl02229-750ppb-1
13 060999013
tox120, rb)02229-1000ppb-1
14 060999014
methanol blank
15 060999015
tox120,106F-G 1-250ppb-m s, 2/22/99
16 060999016
to x 1 2 0 ,106F-G1-250ppb-msd, 2/22/99
17 060999017
tox120,3M -G 1-250ppb-m s, 4/19/99
18 060999018
tox120,3M -G1-250ppb-m sd, 4/19/99
19 060999019
tox120, rbl02229-250ppb-cc1
20 060999020
methanol blank
21 060999021
to x120,153F -G 4,2/22/99
22 060999022
to x120,154F -G 4,2/22/99
23 060999023
to x120,155F -G 4,2/22/99
24 060999024
tox120, rbl02229-250ppb-cc2
25 060999025
methanol blank
26 060999026
to x120,157F -G 4,2/22/99
27 060999027
to x120,158F -G 4,2/22/99
28 060999028
to x120,159F -G 4,2 0 2 /9 9
29 060999029
tox120, rbl02229-250ppb-cc3
30 060999030
methanol blank
31 060999031
to x120,57M -G 4,4/19/99
32 060999032
to x120,58M -G 4,4/19/99
33 060999033
tox120, 59M -G 4,4/19/99
34 060999034
tox120, rbl02229-250ppb-cc4
35 060999035
methanol blank
36 060999036
to x120,61M -G 4,4/19/99
37 060999037
to x120,62M -G 4,4/19/99
38 060999038
to x120,63M -G 4.4/19/99
39 060999039
tox120, rbl02229-250ppb-cc5
40 060999040
methanol blank
41 060999041
to x120,169F -G 5,2/22/99
42 060999042
to x120,170F -G 5.2 0 2 /9 9
43 060999043
to x120,171F -G 5,2 0 2 /9 9
44 060999044
tox120, rbl02229-250ppb-cc6
45 060999045
methanol blank
46 060999046 to x120,173F -G 5,2 0 2 /9 9
47 060999047
to x120,174F -G 5,2 0 2 /9 9
48 060999048
to x120,175F -G 5,2 0 2 /9 9
49 060999049
tox120, rbl02229-250ppb-cc7
50 060999050
methanol blank
51 060999051
tox120,73M -G 5, d 2 5 0 ,4/19/99
52 060999052
tox120,74M -G 5, d 2 5 0 ,4/19/99
53 060999053
tox120,75M -G 5, d 2 5 0 ,4/19/99
54 060999054
tox120, rt>l02229-250ppb-cc8
55 060999055
methanol blank
56 060999056
tox120, rb!02229-H2Oblk-2
57 060999057
tox120, rbl02229-Liverblk-2
58 060999058
tox120, rbl02229-5ppb*2
59 060999059
tox120, rbl02229-10ppb-2
60 060999060
tox120, rbl02229-25ppb-2
Pagel
MS File
PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS -PFHS PFHS PFHS PFHS -PFHS PFHS PFHS -PFHS PFHS PFHS PFHS PFHS -PFHS -PFHS PFHS PFHS -PFHS -PFHS -PFHS -PFHS -PFHS -P F H S -P F H S -PFHS -PFHS -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S
In le t F ile
. pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l p fb s l pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l p fb s l p fb s l p fb s l pfbsl pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l
-
000731
B o ttle
91 91
1 2
3 4 5
6
7
8
9
10 11
92
12
13 14 15 81 92 16 17 18 82 93 19
20 21
83 93
22
23 24 84 94 25 26 27 85 94 28 29 30
86
95 31 32 33 87 95 34 35 36
88
96 37 38 39 40 41
A iiiih k U M
Massiynx - Sample List
X M U / n f t l - ___________ :
Sample List C:\MASSLYNX\amelia-rats.PRO\SampleDB\060999a.SPL Printed: Wed Jun0915:01:191999
File Name
61 060999061 62 060999062 63 060999063 64 060999064 65 060999065 66 060999066
File Text
10X120, rb)02229-50ppb-2 tox120, rbl02229-100ppb-2 tox120, rb!02229-250ppb-2 tox120, rW02229-500ppb-2 tox120, rblQ2229-750ppb-2 tox120, rb!02229-1OOOppb-2
v/v/vy
/y ? ? a
Page 2
MS File
PFHS PFHS PFHS PFHS PFHS PFHS
Inlet File
pfbsl pfbal pfbsl pfbsl pfbsl pfbsl
Bottle
42 43 44 45 46 47
000732
A n tiO i M S A r i fr/a/91______
Tuning Method Report
Method:
C :\MASSLYNX\AMELIA-RATS.PRO\ACQUDB\PFOS
Printed:
Wed Jun 09 1 5 :0 1 :3 5 1999
0/1/9? '
/n & ?
-
Page 1
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 15 5
0.2 150 250
2.98 -60 -5
150 251
Pressures
Analyser Vacuum Gas Cell
Rdbk
1.4e-4 4.9e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0 2.0 > 0.0 56 650
Set Rdbk
15.0 15.0 30 2.0 0.0 40 0 650
47
37 44
000733
Amtitk M W
SM U
Scanning Method Report
Methods
C.-lMMSLXKXVMSKUh-RhTS .PRO\*CQUDS\
taat Modified: Wad Jun Of 14:17:1? I M S
Printed:
ad Jon 0 19:01:0 199
/a M
-ms
Solvent Delay ( mlna ) i
0.00
function I 1
M8M of 2 Mass t a i n ( ISP- )
Inter Channel Delay { Sees ) t Span.( Daltons ) >
Start Ties ( Nina ) i End Tina ( Xina ) :
Repasts t Channel Parent Daughter
0.03 0.00 0.00 13.90
1 Dwall (Secs) Coll tnsrgy (aV)
Co m
<V )
1
299.00 0.00
0.00
2'
299.00 99.00
0.40
49 45
<0 60
funotion s 2
M M o f 2 Maas Pairs ( ISP- )
Inter Channel Delay ( Secs ) t Span ( Daltons ) > start Ties ( Mins ) i End Tina ( Mins I : Repeats i
Channel Parent Daughter
0.03
0.00 0.00
UeSQ
1 fiNill (Sacs) Coll Energy lev)
Co m
* (V )
1
399.00 10.00
0.40
2
399.00 99.00
0.40
43 45
60 60
Saga 1
#
000734
r fiv. i ; u r t i A K ^ v i L W SUMMARY FORM Date:
M ila 0(34*8: quantitad and p rin ta d b y bo} < /1 0 /M
01 (*J U M
l) WA A*Al *f l* UA**U*t *4 H OUVUl<
d1 UW NWHW0 UV M0 -M4M0MU IH4
M A U * U N #-* O 10 O I A 40A
oooo oA<4
ooo se o e
e oe eooc o o o o e o4 c
004 10 to I
IOI4WU410to V 4UUUUMMNMMe o0e4
,
10(0 O 10 oo oto
tow oov OO O Oc AOVKI
sa* 1 X 4
HOOOa
Oo
AA
OO
to IA
to 10
to 10
10Atlft
O .O
IO O
10 O
Oo
Oo
to OI 01
.ACO*
eoooooooo oo<
o o o o o o o ______Ae__o_A_e__
10(010101010101010101010
10101010101010'' "> 1-0 1-0 1 1*
VtftfVMAIOV OOOOOOO
e-- e>ioo oio rid
Ul (0 A A A A *
M O IO <I I*U N H 0
oooo
AA O10 1O0 10 o
O o
O 10 O 10 10
1o0 1o0 uu
e
o
U
io
oc
Ul
i0 m W A i
3 0 0 0 0 0 0 0 0 0 OOOOOO
o" to to>
WAe0MloA0IoOoAAw0OIwoA0lOvoAOM0i
MOpoA0ItMoA0OatIoA0fwPA0o1O0
A O 10 to to MO
A O to to to OM
A O to to to ON
AA OO 10 to 10 to to to ON ON
A O 10 10 to OH
lUNHOVBslAU) 0UNHOW
O A O to to to
HO O
OOOOOO AAAAAA OOOOOO to to to to to to to to to to to to to to to to to to
OH OH Oh Om Oh Oh *JO O0UM
OO Ao oA 1010 to to 10 to OO
OOOOOO AAAAAA OOOOOO to 10 10 to 10 to to to to to to to to to o io to io OOOOOO OOOO OO oO oO oO to O JA U A U M M
sssssssssssr t r t r t r t r t r t r t r r n r r r f
MeoNMooMoMoNMoNoMoMoMe aTNoHMMoMMeoMN
ooooooooooo
HTO UJTOT0 UTM THO OT jTaTMbTUs TMK THR
. 1 1 MtTK . . . . J MMM H (
MMMN
ft M
ff KKKXftXrbKr Xon rXot WfitbrXf orMr brMt ofMt MiftotMt foKt Mo<*oXr t OMNMMN
g oopogo
ftrtv tftftB ftftftftftrrre rtftftft
t|f9B i8faM08Ma0M8M*0MM80MMS0MM9OtiMSOra M8HOa M8HaOM8MaOMSMOMM8OM8MOM8NOHaM8OMa8MOMa8MOH l
i
t
s & S t s s S Uu2 *UwiUMrM.fNf1 uM0u0MuMeJ rM(MHr
Wt*MHHH1HHNHH'
IB H *
i %
a
rs r
!CSC
f
c cc
CO
ta a U a 0S2499 : q u a a tla ta d and p rln ta d by i a j 9/1 0 /3
ffT T Y T T T T T T
IP MI M1 M1 N1 M M M KM MI
,&ea
?ssg
Ari sIHW0WsHVIUH\0>
MMM M S S . S
ga I " " " f t S t 4ttv
mhOOmm Omh Omh Omh Omn Omm OmmQmhOmhOmhfthh5ftH
K K K K K m KKKKK"'^ 5A Ai f Y f Y Y Y Y f Y Y f
E * E.Sl Ht 1*M
Ut} 58S
I
. .I rc s !
S SS t l~
r ?ir }ifai" 3 S Il 0X0] Sa S s is ! M ft Oft :: vv pi
mjp o 9 O
tilllllli--- 5553 33333hIiII3333H I H f ! H i i 8 E i 8 r ' H s r r , H S { * H E r , E i U i { H H i r i i f 3333 3333 3333 3333 3333 3333
HsytMH
SSSSSSS
SSSSS8888
I
' '
> W
S8
M
8
ggg
MM
8 .8
W
.8
gggg
< t t v w w w w www o
|S3g i i g s ssss ssss is s g -s
WM
88
g
HftdINH
.8 8 8 8 8 8 8 8 .
ggggggggg
# < w w w w w w w w w w
s gsssg sssssgsgsl
o I
3
iS S S S s b
" H i " S = s s s : ; 3 SSSs st . s s H
^ S s s s a s
S
O
O
I s S s S s s S S S i ' s B S s H i t s s s 5 5 =
s ' : l l ' i I SSs s s
O
sssssss
Sags sss s s ts 58So sas? s
bssss ssssbcbsss
>3
ggggggg . gggg gggs ggg gggg gggg g
g ggggg gggggggjfg I
CO
WI HHt HfIt fIt O O f t
MoI . rfj- MHI
HO I
UI
H
MM l m
w
I WUtMf HuOHwM
?
f e } > L > fe j-
_________________________ ______________________ _ ______________________________
a u itie ir CaU bcm tlon lU p o rt
t U O ,
and f O i
fan 1
C a l l t e a t i o n i O V a M M n o ftaaar t l a B d . n a v e t nOOVOOOOW*
l u t a o d lfla d i I t a Jttn 10 0 7 iU il7 1999
T rlatadt
I t a Ota 10 0 7 itt< 3 S 1199
t a l i 0<34M> q u a n tla ta d and f r l a t a d b y b a l 9 /1 0 /9 9
000738
