Document zbpK7rXq6Dk8E3Y2637Zr5xjz
TOXICITY TO TERRESTRIAL PLANTS ARR AGE -- 1368
TEST SUBSTANCE
Identity: FPeCr-f9l5u.or(oo1c-tOancetsaunlfeosnuatlef;oamcnaiidyc,a1l,s1o,b2,e2.re3f,e3r4r,e4d,5t,o5a.s6.P6.F7O,7S.8o8r8-
heptadecafluoro-, potassium salt, CAS # 2795-39-3)
Remarks: The test substance is a white powder. Sample was taken from 3M lot number 217. Sample was stored under ambient conditions prior to
etelsetminegn.taPluraintayldyesteesrtmeicnhendiqtuoebse. 86.9% by LC/MS, "H-HMR, "F-NMR and
METHOD
Method: The study was conducted using a protocol based on procedures
oOurtglainniezdatinioUn.Sf.orEEPcAonOoPmiPcTSCoGoupiedrealtiinoensa8n5d0.D4e1v0e0loapnmde8n5t0(.O4E22C5D)and the proposed revision of Guideline No. 208. GTeLsPt:tyYpees: Artificial soil Year Completed: 2003
Species Monocots: Allium cepa (onion -- `Texas Grano') Lolium perenne (ryegrass -- `Manhattan 3')
Dicots:
LMienduimcaugsoitsaattiisvsai(maulmfal(ffaa)x)
Lactuca sativa (lettuce -- `Summertime')
LGlyyccoipneersmiacxon(essocyubleeannt-um`G(rteoemnatEon~vyR'u)tgers')
ALnCalMyStiicnasloimloantitDoaryisng0:aTnedsttessutbtsetramnicneatcioonncaenndtriantipolnanstwaenrde
measured
(when
by
possible) fruit tissues at test termination.
r`Setgarteisstsiicoanl amneatlhysoidsso:f EBrCuvcaelaunesd cVaelrcsutleaetegd,,owrhweitnhpnoosns-ilbilnee,arbyinntoenr-ploilnaetaiorn cusoimnpgatrheedItCopcaopntprroolamcehaonfsNowribtehrgD-uKninnegt.t'sTtreesatt(maen=t0.g0r5o)upusmienganSsASwere Version 8 to determineifsignificant differences in emergence, survival, weight or height from the control occurred.
Test organism source:
Onion:
Territorial Seed Co., Cottage Grove, OR
Ryegrass: Meyer Seed Co., Baltimore, MD
-
Alfalfa:
Frontier Natural Products, Norway, IA
Flax:
`Arrowhead Mills, Hereford, TX
.
Page 1 of 12
002181
Lettuce: ~~ Temitorial Seed Co, Cottage Grove, OR
Soybean: Tomato:
MJoehynenry'SseeSdelCeoct,edBaSleteidmso,reA,lbMioDn, ME
Test organism age Test conditions
at
study
initiation:
seed
Test media: Artificial loam soilofthe following composition:
Kaolin clay, industrial quartz sand and peat in a ratioof 2:25:1 by
weight. Crushed limestone (to buffer the pH) and a slow-release
fertilizer were silt, 21% clay,
also and
added. Final 2.1% organic
mix consistedof matter. Soil pH
49% was
sand, 7.79.
30%
Environmental conditions: Study conducted in a temperature-
controlled sunlight to
gprreoevnihdeouaseu.nifArotrimfic1i4al-hloiguhrtipnhgotsouppeprlieomd.ented
natural
Stock and test solutions preparation: Approximately 70 kg of
seeapcahratteeslty.coTnhceenttersattisounbsatnadnctheewnaesgaftirisvtemicoxnetdrowlitwhasa
prepared 1000 g aliquot
ocfontthaein7i0ngkgaolfiqtueosttwsoaisl (tshoeilnmaodidsetudrbeaocfk1t5o%t)h.eT7h0ektgesotfsbuublsktsaonicle-
ianntdhmeisxaemdefomra1n0nemri,nubtuets.noTtheestnseugbasttiavencceonwtraosl asdoldledw.as prepared
Exposure vessels: Plastic pots, approximately 16 cm wide and 12
cm deep.
Number of replicates: four
Number of seeds per replicate: ten
Number of concentrations: five plus a negative control
Watering procedure: subirigation
Test conditions parameters:
Temperature range: ~~ 14.1-32.0C
Relative humidity range: 8-91%
Exposure perLiiogdht(gientremnisniattyiroannagne:d grow1t2h.8te2st6).:62m1o-lDeasysPAR
Exposure period (extended test): up to 205-days (variable by
species)
After observations on day 21, all but one plant was clipped
from each pot. The remaining plant was maintained in the
`extended test, until (if possible) fruit formation. At test
vteergmeitnaattiivoen,titshseueosnweeprleanatnwaalyszehdarsveepsatreadtealnydfotrhePfFrOuiSt.and
Element Basis: ECzs, ECso, NOEC ~ emergence, survival, shoot
Ohetihgehtr,osbhsoeortvawteiioghnts: seedling condition
Method of reporting test concentrations: nominal
RESULTS
Nominal concentrations: Bk control, 3.91, 15.6, 62.5, 250, 1000 mg/kg
Initial measured concentrations: <LOQ, 3.61, 11.1, 50.8, 276, 998 mg/kg
002182
Page 20f 12
Element values:
21-Day Emergence, mg/kg
F-- h -i2o 55 a0 no-T-0 Lae [Species TLOECTENCOs[EECCwT|
[Onion 17250[625|51 |210| [Ryegrass| 250|625|200|340|
[Flax |
[250|
[Lettuce |1000|250|390|560|
[Soybean Tomato
||>11000000|"1205000||>1301000|]>4170000|
21-Day Survival, mg/kg [SpeciesTLOECTNOEC[TEECCs :| [Onion "6251 | 7471|6576| [Regrass| 250 |625|170|310|
[Aflalta "250|625|250|450 |
[Flax_T7--62"5 |117402| 52300| [Lettuce 17250T7625|260|390| [STooymbateoan ||>1602050|]1105060|[>16090|0>1100500|
21-Day Shoot Height, mg/kg [Species TLOECTNECOsE[ECCsT| [Onion 17625|166|20|47| [Afalfa 17250 "625|100|250| [Flax 17250 [7625| 98 |140| [Lettuce |3.07 [<597]68|40|
Tomato | 625 | 166| 22 | 94
002183
Page3o1f2
21-Day Shoot Weight, mg/kg
[Species [LOEC [N|EO CzsE |ECC so| [Onion [7156|391 |13|28| [Ryegrass|156| 3.91 | 7.5 |54| [Alfalfa "250|625|53|150| [Flax [7250|625 | 82| 120| [Lettuce |391[<397]89|20| [Soybean |250 |625 | 160| 330|
Tomato
625 15.6
12
29
All element values were calculated using nominal concentrations.
Statistical Evaluation Variables:
SEumrevrivgaeln:ceN:umN beruofm olifvb iengme eemregr regdesdeesdeleidnlgisngpserpe1r01p0lasneteeddsspeeerdspotp.er pot. Weight: Mean shoot wet weight of living emerged seedlings in each pot
Height: Mean height of living emerged seedlings in each pot.
Biological observations:
Onion - Day 21
MEsom=Rms E0[a[EnooE s|Eeon oa [| oe| ea m oeo" mm,] CeereTTooTToollooww[|oooeToet [|oemeenn|| CCaoeTTeoTeoTowT Tooe a a| ae] Ce wTo[oe e ToT]
Sm wns Ryegrass -- Day 21
Le,
CaeTwTo ew |r|oe|
[FieL tTe t ose Toe rTLe we |t oo |e0r |e] m|a
"Statistically significantly different from the control (p<0.05)
00218
Page dof 12
ler a=] Alfalfa -- Day 21
Procol 0Tw[on[00 [42 | nesomarn| FooTwTw on[oo[20 | ww | [ee Tw[ol oo[o [ 60 | wewsm | [ee To[ol oo[ or [ 00 | wesossrn |
[mo [on Jol ow [or[0 [| smn |
Low | so Tol ow | wo | 7% | essai |
TA scoreof 0 = normal seeding; 100 = dead seeding
=e ea Flax-- Day 21
[mom | 50TnToo| 00 | 14 | vomssmosconnn| [oo To[ol on | wr | or | rose | [ose | wo Tw ow[oo 2 |oumsumssconn] [ee | ow Tolow[wo| 0| tocar | Foo ToTolow [wo | oo uses] Lowe | ooTo |TT = 7]
"Statistically significantly different from the control (p<0.05)
Lettuce -- Day 21
Slee Tea [meres] 0[wl ow[ 00 | 0| ww |
FooTwTw ow [ao [0| ww | [oe TwTw ow[ao | 0 | eon| [oe |wTwl ow |20 | 5 | neon|
= ee To|el o=g| LomLowLor[re[ ow|v [oan]
Page Sof 12
Soybean - Day 21
Concaneosoion
Frosh Weight
pi
"
[pom | woTwo| soo | 20s |0| tem |
FonTwTw| su | oe |on| tose |
[oe |ow [owl ww |or | to | toon |
T"SAtsaticstoicorafle0si=gninfoircamnatllysdeiefdfienrgo;t 1f0o0m=thdeeacdonstreoeld(ipn<g0.05)
Necro LSalma)t
Tomato- Day 21 Concane=aton
Frosh Waight,|| Heaight | Cosnedeitrison | 0
co
[woTofel oo[ww [oTgNecyro 3] |
[oe | wo [ol ow [ 26 | 0 | emer | Foo [ow [ool ow | a0 | 0 | wesw|
"SAtastciosrteicoalf0si=gnniofrimcaalntsedeidfeirnegn;t 1f0r0om= tdheeacdonsteroeldi(npg<0.05)
Control response: Satisfactory for all species
Extended test observations: There were no additional findings of phylotoxicity attheterminationof the extended growth portion of the test.
Analytical Methodology: Analyses of test soils and plants were
cpherrofmoarmteodgraatpWhiyldwiifteh ItrnitpelrenqatuiaodnralupLotld.e umsaisnsg hsipgehctpreormfeotrrimcandceteelcitqiuoind
s(uHbPsLtCa/nMcSeIMinS)t.heWshamepnledse,tetrhmeisnianmgetahendcomnocsenttprartoimoinnoefntthpeeatkestresponse
ofonr tpheerfblausoirsooocftianndeisvuildfuaolnaitseomwearsicucseodm.ponNeontast.temTpthewaLsOmQa(dliemittoofquantify
.
quantitation) in soil was 1.18 mg/kg. For vegetative tissue, the LOQ 002186
.
Page of 12
ranged from 2.4-- 7.6 mg/kg. For fruit, the LOQ ranged from 0.12 6.4 mag.
Tanhaelyrseicsorvaernygeodf mfartormix83fo.r9ti%fi--ca8t9io.n0s%anianltyhzeeSdoiclo,n8cu6r.re3n--%tly1d1u7r%inginstahmepflrueit
and 88.0% -- initiation had
110% in the vegetative measured values from
tissue. 71.2%
Soil samples collected to 110% of nominal.
at
test
Measured values for 10 62.8% of nominal.
samples
taken
at
test
termination
ranged
from
22.8%
Values reported concentration.
as
<LOQ
were
excluded
from
all
calculations
of
mean
Summaries of Analytical Chemistry Data (All results reported as dry weight):
Soil, Day 0:
Concantaton.
ota
Concanvaton| Nome
[oo | swamase | sa | mo| [se | wae [wn | me] [as| wnwme | son |es|
[Cm| wom | ws |wo|
00 = 1.18 mgkg
Soll, termination (Day 205): CoNnocmainniaatToesnt,| MassurodRmoopikcaat Vaues,
a)
Concawnoina|ton, | Po`rNcoarnrtaor moa
[on | [me | [oe |
|
Q = 1.18 malkg
vw ewan wow ww
| um | wo| | sw | me| | we | me] |ow |
002187
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Onion Vegetative Tissue, Termination (67-days):
TTaomoeeerr |T | onmwseiewa on [| me ee] enn]| Meet em ------------ a]
Onion Fruit, Termination (67-days)
[ ammo |scocve oorsmmereseT || o2t0 || Concentration,
mghg
Concentration,
oe| wormrme | mi |
oo| voor |]
'LOQ = 0.42 mg/kg
Jn = nn Ryegrass Vegetative Tissue, Termination (205 days)
e ae TrowenmeT n [|oaew || oo | wows | wn]
Ryegrass frui--t No sample (did not mature by 205 days)
0021;
58
Page 8of 12
5 cen Alfalfa Vegetative Tissue, omeTermination (141:r;cdays)
[so |doodo|neew e| [ss|swam|ac a2a| [oe | msensTo]
oe Alfalfa Fruit, Termination (141 |days) ee| [nogecose| mac | woo |
[ws | oom | won | | 2s |coow|mwmom|
[mw | oocomo | aon | oon | mse |]
[m[Sg erdae risrmem Flax Vegetative Tissue, Termination (94 da)
[me | oumamo | we | [es |snmu |w wos|
LOQ = 2.4 mghkg
VOR159
dreemass | mamsewswsaemrn| Flax Fruit, Termination (94 days) iromn CFo oo|om weor m||oaeomm]| Teese |TT mvemsemmeann | | oowm]|
dai Lettuce Vegetative so Tissue, Termination Er (67 days) Coooe T 1 wwroerwmresn | | ewen]| CToor| | rweasrome | |a] w |
'LOQ = 5.5 mg/kg
Jos Soybean Vegetative Tissue, Er Termination (67 day C[oi T n mroaewo m | |mw] oen]] CTaoee| T wwoenenes | |aan]] [Cooe| | epaomeemsT| ooe]|
.0Q = 6.0 mg/kg
VoZ150
Page 100f 12
Soybean Fruit, Termination (67 days)
oa Test
Tisuo Vas,
a
a
| so |oomom|eizw n| [se[ow|eomn e |
[mo [ wsm |aoao| [m76ao= 0.1| 8 maka vomcomssre|. |
Tomato Vegetation, Termination (day 94)
Concanaton,
rota
Concanesion,
[topmm aceoo|tason||
[om[wae [7 [me [ wam|es wes|
ope 0a= 78mg
Tomato Fruit, Termination (day 94)
Corcoran.
oo
Concorso,
[tomsmao ce co |waa on || [so [aonconm|mmcmoome| [we| woausom | os | [2s [| oomac|awooow a|
hw6ao= 047|maha owe ||
002191
Samplesof soil and plant tissues from all species were also obtained at
.
Day 21 and analyzed for PFOS. However, plant tissues and soil obtained
on Day 21 had considerably higher than expected PFOS concentrations. Day 21 soil samples were collected by scraping the surface of the soil,
Page 110f 12
twehremrineaatsioan.soTilhecolraebosraamtpolryercownacsluudseeddtfhoartsPaFmpOlSescocnoclelnetctreadteadt tienstthe top.
layerofthe soil as a resultofwatering collected atDay 21 were very small in
by subirrigation. size, and likely
Plant tissues contaminated with
vsuarlfuaecsewseoriledcuoenstiodiemrperdopteorbceolnloetctrieopnretseecnhtnaitqiuvees,.anTdhewresrfeorneo,tDraepyor2t1ed.
CONCLUSIONS
eee
sTpheecipeostoafssteirurmespterirafllupolraonotcstfaonretshuelffoonuartset2u1d-ydeanydpEoCisnotsrawnegreefofrosuendvetno be: Emergence: 210 -> 1000 mg/kg Survival: 57->1000 mg/kg Height: 40-460 mg/kg Shoot Weight: 20-- 330 mg/kg
The 21-Edmaeyrgneonocbes:er6v2e.d5e-ff1e0c0t0comngc/enktgration ranges were found to be: HSuerivgihvta:l: <31.59.16-6120.050mmgg//kkgg Shoot Weight: <3.91- 62.5 mg/kg
Submitter: 3M Corporation, St. Paul, Minnesota, 55133
Environmental
Laboratory,
P.O.
Box
33331,
DATA QUALITY
Reliability: Kiimisch ranking 1.
REFERENCES
This study was conducted at Wildlife requestof the 3M Company.
International,
Ltd.
Easton,
MD
at the
OTHER
Last changed: 6/3/03
LvuULlY2
Page 120f 12