Document zbo7XKxqKDqerVV2MLVR51Bpg

FOR D PONT USE ONLY Copies to AR226-2898 E a I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 352-81 MR NO Haskell N o . I 14,008 Other Codes Study Initiated/Coimpleted 6/4/81 - 6/6/81 terial Submitted bv Polymer Products Department Wilmington, Delaware 48-HOUR LC50** TO DAPHNIA MAGNA Procedure: The test material, as a 1 mg/mL stock solution in laboratory test 'wter, was diluted with laboratory test water to yield the desired exposure concentrations. After mixing, 200 m L of each concentration was Introduced into each of two separate 250 m L glass exposure vessels. Two identical jars, containing only laboratory test water, were designated as controls. Ten daphnids (Daphnla magna) less than 24 hours old were randomly assigned into each vessel. Food was not provided during the test. Test solutions were not aerated and temperature was maintained at 208C. Photoperiod was maintained at 16 hours light:8 hours dark. Mortality counts and observations were made at 24 and 48 hours after the exposure was initiated. Dissolved oxygen and pH were measured in the control, low, medium and high exposure solutions at the beginning and end of exposure. The total alkalinity, hardness (EDTA) and conductivity of the control were measured at the beginning of the exposure. The 48-hour LC50 and 952 confidence limits were calculated by probit analysis (Finney, 1971).t Results i Percent mortalities for Daphnla magna exposed to H-14,008 are presented in Table I. The 48-hour LC50 was 320 p pm with "952 confidence limits of 281 ppm and 362 ppm. The slope, of the dose response curve was 5.1 with 952 confidence limits of 3.7 and 6.5. At concentrations of 560 ppm and greater the test solutions were too cloudy to complete an accurate mortality count at the 24-hour observation. No daphnids were observed swimming in the 1,000 ppm vessels, therefore, mortalities were noted at 24-hours by l- Company Sanitized. Does notcontan TSCA CS withdrawing each daphnld via a manually operated suction tube and observing the animals* The chemical and physical parameters measured during the test are reported in Table II. SummaryT^ ^ ^ M B I ^ M I ^ ^ B M M M B ^ ^ ^ ^ E x h i b i t e d s li g h t acu te t o x i c i t y to Daphnla magna under static, unaerated test conditions during a 48-hour exposure. The 48-hour LC50 was 320 ppm with 95Z confidence limits of 281 ppm and 362 ppm. The slope of the dose response curve was 5.1 with 95Z confidence limits of 3.7 and 6.5. * Other Names: PurityI Contaminants y ** LC50 The concentration which is lethal to SOX of a test population during the specified time period. t Finney, D. J . , Problt Analysis, 3rd ed., 1971, Cambridge University Press. Report by : / n a r h ljifc < * Robert L. Trlvlts Technician Study^Wreccor: / /& + , ' Frank X. P h i l l i p Research Biologist Approved by: ____ RLT:FXP:CDL:jrg Date Issued; June 18, 1981 Chief, Aquatic Report No. -2 Compaq Sanili*05, Does noi contain s scag mm TABLE I RESULTS OF A 48-HOUR ACUTE iXICITY TEST WITH DAPHNIA MAGNA EXPOSED TO H-14,001 Nominal Test Concentrations ppm 1000 750 560 420 320 240 180 100 Control Observed Mortality (Z) 24 Hours 48 Hours A* B AB 100 100 ** ** ** ** 00 00 00 00 00 00 100 100 100 100 100 100 60 50 40 30 20 40 20 20 00 00 * Replicate exposure chambers containing ten daphnids each. ** A few daphnids were observed swinning, but the test solution was too cloudy to complete an accurate mortality count. f TABLE II RESULTS OF PHYSICAL AND CHEMICAL PARAMETERS MEASURED DURING A 48-HOUR ACUTE TOXICITY TEST WITH DAPHNIA MAGNA EXPOSED TO H-14,0081 Nominal Test Concentrations 1000 ppm (High) Dissolved Oxygen (ppm) 0 Hr. 48 Hr. 5.6 6.6* H 0 Hr. 48 Hr. 7.9 8.1* Total Alkalinity (mg/L as CaCO^) 0 Hr. - EDTA Hardness (mg/L as CaCO^) 0 Hr. Conductivity (umhos) 0 Hr. 420 ppm (Medium) 100 ppm (Low) Control 6.8 7.8 6.5 7.4 8.1 7.7 8.0 7.9 8.3 8.3 7.9 8.1 - - 133 - - 117 265 Final dissolved oxygen and pH were measured at 24 hours because 1002 mortality had occurred within the first 24 hours of exposure. - 4Company Sanitized. Doss not contain TSCA CBS