Document zb9LNzg4aMY62087Gvgm0Gorg

ACUTE TOXICITYTO AQUATIC INVERTEBRATES - Ceriodaphnidaubia TEST SUBSTANCE IdentityN:-Ethylperfluorooctanesulfomnaaymaildseo;be referrteodas U 1464, ETFOSA, ETPOSA, F-6309, orFX-1 2. (1Octanesulfonamide,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluo ro-N -ethyl-C,AS # 4151-50-2) Remarks: Materialisa white solid.Sample purityand lotnumber were not recorded. METHOD Method: Adapted from OECD 202 - Water accommodated fractions Type: Staticacute GLP: No Year completed: 1998 Species: Ceriodaphniadubia Analyticalmonitoring:Nominalconcentrationosnly.Temperature,pH and dissolvedoxygen recorded Statisticamlethods: :EL50 (lethalloading)valueswere calculatedusing the probitmethod. The NOEL was calculatedby Fishees ExactTest, TOXSTAT SoftwarePackage, Version3.5,Laramie,Wyoming. Test daphnid source:.AScl in-housecultureD,uluth,MN Test daphnid age at study initiation<: 24-houroldneonates Test conditions: Dilutionwater: Carbon-treateddechlorinatedCityof Duluth,MN tap water aeratedfor24-hourspriortouse inthetest. Dilutionwater chemistry during the study: Hardness 40-42 mg/L as CaC03 DO: 8.5-9.3mg/L Alkalinity: 42 mg/L as CaC03 pH: 7.8-8.0 Conductivity 118-125 pmhos/cm Test Temp. Range: 21.2-21-.3 Stock and testsolution preparation:Test solutionsprepared by mass additionoftestsubstance to 1.5Laliquotsofdilutiownater (one perconcentration)T.he solutionwsere vigorouslystirred (vortex1/3to 1/2solutiondepth)for20 hours. The aqueous phase was siphoned from the vesselatmid-depth. Exposurevessels:30 mL borosilicgaltaessbeakers Exposurevolume:20 mL Number ofreplicatefso:ur Number ofdaphnidsperreplicatfei:ve Number ofconcentrationFsi:veplusa negativceontrol Elementbasis:mortality RESULTS Nominal Element loading concentrations: Bk control7,8, 130,216, 360 and 600 mg/L value: 24-hour EL50:380 (330-438)mg/L 48-hour EL50:328 (278-391)mg/L 48-hour ELo: 184 (130-224)mg/L 48-hour NOEL: 216 mg/L Mortalityof controls: 0% after48-hoursexposure Remarks: Allvaluesreportedare fornominalconcentrationsN.o measurement oftestconcentrationswas conducted. Percent Mortality Nominai Loading Concentration,mg/L 24-Hours 48-Hours Negabve Control 0 0 78 0 0 130 0 5 216 10 20 360 25 40 600 100 100 Remarks: Allvalues reported are fornominal loadingconcentrations. No measurement oftestconcentrationswas conducted. CONCLUSIONS The testsample 48-hour EL50forCeriodaphniadubia was determinedto be 328 mg/L witha 95% confidenceintervaolf278-391 mg/L Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY ReliabiliKtlyi:miscrhankin3g.Thisstudylacksanalyticdaaltafor determinationofthetestsubstance concentrationinthe testsolutionsand determinationofthe sample purity.Testingwas alsodone wellabove the solubililtiymiotfthetestsubstance withthe use ofwater accommodated fractions. REFERENCES Study conducted atthe requestof3M Company by,AScl Corporation, Duluth,MN. OTHER Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 Last changed: 5/18/00 ASci Corporation/AScl-Duluth EnvironmentaTlestingDivision Submittedto3M Co. -5/98 ASci Study ID# 5010-068 STUDY =E ACUTE TOXICITY OF U1464 TO CERIODAPHNL4 DUBLI DATASTANDARD ADAPTED OECD GUIDELINE 202 STUDY COMPLETED May 4, 1998 TESTING FACILITY ASCI Corporation/AScI-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,MN 55811-5712 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATIOli NUMBERS ASCI Study ED# 5010-068 3M Company Lab Request# U1464 Sponsor. 3M Company Sponsor Study ID# U1464 ASel Corporation/ASci-Duluth Environmental Testin@.Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 1.0GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# TestingFacility PrincipalInvestigator ProjectManager TestingFacilitDyirector DefinitiveTest Dates Data Validationand Report Review AcuteToxicityof U1464 to Ceriodaphnia dubia. - Adapted OECD Guideline202. 3M Environmental Technology and Services, Building2-3E-09, 935 Bush Avenue, St.Paul, MN 55133. Rochelle R. Robideau 3M Environmental Technology and Servi'ces,Building2-3E-09, 935 Bush Avenue, St.Paul,MN 55133. Tel No. (612)778-7065. U 1464. AScl Corporation/AScl-DuluthEnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811; Tel. No. (218)722-4040. Todd McGuire. Joe Amato. Donald Mount. April 15-17, 1998. Alan Mozol. Report Signatur Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Two years from the datestudy iscomple@ed. ASCI Corporation/AScl-DuluthEnvironmental Testing Division,4444 Airpa-rkBoulevard, Duluth,MN -55811;Tel.No. (218)722-4040. Sponsor: 3M Company Sponsor Study ID# U1464 2 AScl Corporation/AScl-Duluth EnvironmentalTesting Division Submitted to 3M Co. - 5/98 ASci Study ID# 5010-068 2.0 OBJECTIVE(S) To determinethe48-h EL's (effectivleoading'sE)L,o and EL50, and 48-h NOEL (no observed effectloading)of the testsubstance,U1464, for Ceriodaphniadubia. This studywas conductedaccordingto the AScl studyplan retainedby theSponsor and an adaptationof OECD Guideline202 (OECD 1993). Test solutionpreparatiownas wateraccommodated fraction(WAF) per Girling& Whale 1994. 3.0 TEST METHODS Test Substance Test Organisms DilutionWater Exposure Chambers Properties:U1464, whitecrystalss,pecifigcravity1.7 (water= 1),stable, and negligiblesolubilitiyn water. Test loadings:0 (dilutiownatercontrol)7,8, 130,216, 360, and 600 mg/L. Each testsolutionpreparedby mass additionof testsubstanceto 1.5-L aliquotsof dilutionwater. Each solutionwas vigorouslystirred (vortex1/3 to 1/2 of flasksd'epth)for20 hoursto obtainWAF'S. Test solutionobtainedfrom flasksby siphoningaqueous phase from mid-depth. Source: Healthy, _:!2@4_-h old Cefiodaphnia dubia neonatesobtained from ASCI individualorganism culturesmaintainedin syntheticmoderately hard reconstitutewdater (MHRW) (hardness80 to 100 mg/L as CaC03). Use in test:Gravid adultsisolatedin MHRW 24 h beforetestinitiation. Used 5 organisms/replicataend 4 replicates/telsotading. Handled with a smooth-bore eye dropper. Did not feed duringthetest. Source: Carbon-treatedechlorinateCdityof Duluth,MN, tap water. The most recentchemicalanalysisof postcarbon (PC) water isin the Raw Data Package. Characte?istics:At testtime, the water had a hardness of 40 mg/L (as CaC03) and a pH of 7.87. Aeratedfor24 h priorto usingin thetest. 7@pe: 30-ml borosilicatgelassbeakers. Use in test: 20 ml of dilutionwater or testsolution/chamber. During the I test,kept covered, exceptwhen experimentalobservationswere made. Sponsor: 3M Company Sponsor Study ID# U1464 3 AScl Corporation/AScl-DuluLh Environmental Testing Division Submitted to 3M Co. - 5/98 ASel Study ID# 5010-068 Incubation Observations Endpoint Calculations Duration: 48 h. Daily photoperiod: 16 h lightand 8 h dark, maintainedusing cool-white fluorescentbulbs. Temperature: 21'C. Biological:Daily- counted dead testorganisms. Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry:(1) At testinitiationt-otalhardness and alkalinity(,2) daily-temperature,pH, DO, and conductivity.All determinationswere made accordingtoAPHA methods (1995). ELo and EL50: EPA ProbitMethod. NOEL: Fisher'sExact Test(TOXSTAT statisticsaolftware,Version 3.5, Universityof Wyoming & West Inc.). 3.0 RESULTS Test SubstanceToxicity(95% Confidence IntervalP)ertinentData arein Table I Ranges of Water Chemistry Parameters L 24-h EL50: 380 mg/L (330-438 mg/L). 48-h ELSO: 328 mg/L (278-391 mg/L). 48-h ELIO: 184 mg/L (130-224 mg/L) 48-h NOEL: 216 mg/L. Totalhardness (as CaC03 mgll): 40-42. Alkalinity(as CaC03 mgll): 42. Temperature (C): 21.2-21.3'C. DO (mglL): 8.5-9.3. pH: 7.8-8.0. 1 Specificconductivity(tLmhoslcm): 118-125. Sponsor: 3M Company Sponsor Study ID# U1464 4 AScl Corporation/AScl-Duluth EnvironmentaTlestingDivision Submittedto3M Co. - 5/98 AScl StudyID# 5010-068 4.0TEST QA/QC QA/QC Criteria Testorganism dead,immobilizedor trappedin controlexposuremust not be more than 10%. The lowestDO concentratioant test terminatiomnust be atleast60% of theair saturatiovnalue attesttemperature. Test durationmust be 48 h. Referencesubstancetoxicittyest(thistestis optional). Data 0% dead,immobilizedor trapped. The lowestDO concentratiomneasured was 96% of theairsaturatiovnalueat21.0*C. The testdurationwas 48 h. CurrentcontrolchartforC. dubia acute testingisincludedin theraw datapackage. 5.0 REFERENCES American PublicHealthAssociation(APHA). 1995.StandardMethods forthe Examinationof Water and Wastewater.APHA, Washington,D.C. GirlingA,.E., G.F. Whale, and D.M.M. Adema. 1994. A GuidelineSupplementfor DeterminingtheAquaticToxicityof PoorlyWater-SolubleComplex MixturesusingWaterAccommodated FractionsC.hemosphere, Vol. 29, No. 12,pp. 2645-2649. OrganizationforEconomic Cooperationand Development (OECD). 1993.OECD Guidelinesfor Testingof Chemicals.OECD PublicatioInnformationCenter,Washington,D.C. Universityof Wyoming & West Inc.1996.ToxstatVersion3.5.Cheyenne, Wyoming 82001. USEPA ProbitAnalysisProgram. Version1.4. Sponsor: 3M Company Sponsor Study ID# U1464 5 AScl Corporation/ASel-Duluth Environmental Testing Division Submia.ed to 3M Co. - 5/98 AScl Study ]D# 5010-069 TABLE 1. ACUTE ............ T 0 78 130 216 360 600 TOXICITY OF U1464 TO CERIODAPHNIA .......... .................... h .......... ti6,, d P 0(0) 0(0) 0(0) 2(10) 5 (25) 20(100) DUBIA f 0(0) 0(0) 1 (-5) 4(20) 8(40) 20(100) Percentages are in parenthesis. EFFECT AND NO-EFFECT LOADINGS WrM CORRESPONDING 95% CONFIDENCE INTERVAIS 'E:Ll@-. d :N.OEj@@ 24-h EL50 48-h ELSO 48-h ELIO 48-h NOEL ............... D 380 328 184 216 95@Y.-. 330-438 278-391 130-224 Not Applicable Sponsor: 3M Company Sponsor Study ID# U1464 6