Document zQwV8e4MwroxZXoO3mQnqd8k7
AR226-2899
FOR DP PONT USE ONLY
E. I. dfi Pont de Nemours end Company Haskell Laboratory; for Toxicology sod Industrial Medicine
Elfcton Road, Newark, Delaware 19711
HASKELL LABORATORY REPORT NO, 541-81
Meterla.
fasskell No. " 1 ; W 8 .
StUd6/8/811*t6/23/811*te^
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" ^ ^ ^ i ^ ^ ^ M u c t s Department Experimental Station 353/318
. 0RAL LD50 TEST IN RATiS
Procedure: The test material * en aqueous suspension, was l nistered by lntragastrlc Intubation In si Ogle doses to 6 groups of 10 young adult Crl:CD* male rets. A Range Finding Study was conducted to'determine the Initial dose level for the LD50 test.** T lie surviving rats were weighed and observed during a 14-day recovery perl yd and then sacrificed The LD50 value was calculated from the mortality data using tie method of D. J. Finney.***
Results:
Dose (mg/kg) 3,700
3,000 2,500 2,000 1,500 1,000
Average Body Height (g)
251
Suspension
----(rf>----
f
Average Dose (mL)
2.32
240 4o 1.80
. 241 243 236 241
1.51
io 2.43 P 1.77
i0 1.21
Mortality Ratio
10/10
LD50**** 2,196 mg/kg
5/10
6/10 5/10
3/10 0/10
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I; Company Sanitized. Does not confa>r> to/' />*'
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Clinical Signs;
:iet and/or stained perineal area, weakness, stained face and weight loss at
all dose levels. Chromodacryorrhea at 3,700 and 2,500 mg/kg. Tremors at
3,700 mg/kg. Lacrlmation at ,000 mg/kg. Congestion at 2,500 ag/kg. Stained
underbody at 1,500 og/kg. Lethargy at 3,700, 3,000 and 2,000 ag/kg.
Diarrhea at 1,000-2,500 mg/kg| All deaths occurred within 7 days after
dosing,
:
Summary:________________________ orally to-young adult aale CrJf:CI ag/kg of body weight.
is moderately toxic when administered ts in single doses; its LD50 is 2,196
Clinical signs observed St moat dose levels included: wet and/or
stained perineal area, weakness, stained face, weight loss and diarrhea, All deaths occurred within 7 days after dosing.
*** AMA
A Range Finding Study slhowed deaths occurring at 2,250 mg/kg when administered at 670 andj 2,250 mg/kg, 1 rat per dose level.
Finney, D. J., Problt Analysis, 3rd Ed.i 1971, Cambridge University Press.
951 Confidence Limits: i Lower: Upper: Slope:
1804 ag/kg
-2652 mg/kg 4.94
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Company Sanitized. Does not contain TSCA CBI
Report by:
7 ..... John E. Henry / Technician
/(pproveo by:
oft.-lA
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Wflld L. Kennedy,vj^.
Chief, Acxite Investigations Section
JEH:jrg:WP:12.6
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Date Issued: September 23, 19(81
kReport No. 541-81
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Company Sanitized. Does not contain TSCA CBf
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