Document zQoNmKvwNKnB5DaOB9eepy5z0

Evaluationof RespirationIn and BOD/COI sheets (Lisalt ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:PerfluorooctanesulfonatLei,thiumsalt;may alsobe referredto as PFOS Lisalt,FC-94, or FC-94-X. (1-Octanesulfoniaccid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecaflliutohriou-ms,alt, CAS # 29457-72-5) Remarks: The testsample isa mixtureofthe testsubstance inwater (approximatel2y4.5% testsubstance and 75.5% water).No calculations were made toadjustfortheactualconcentrationofthe testsubstance in the testsample. METHOD Method: OECD 209 Test type: Staticacute GLP: No Year Completed: 1994 Analyticalmonitoring:pH and DO concentrations. Statisticamlethods: Not performed Test organism source:Activatedsludgemixed liquocrollectefdrom the MetropolitanWastewater Treatment Plant,St.Paul,Minnesota. Test conditions Dilutionwater: MilliporMeilli-Q@w)ater Synthetic Sewage: Per OECD method Reference and testsolutionpreparation:A stocksolutionofthe referencesubstance,3,5-dichlorophenolw,as prepared per the OECD method. The pH ofthe referencesolutionwas measured to be 7.4. The testsolutionswere createdby directadditionofthe testsample;finalconcentratioonf 1000 mg/L. InitiaplH ofthe two 1000 mg/L testsolutionswas 7.1and 7.2. Test vessels: One litegrlassfleakers. Number of concentrations:1 (done induplicatep)lus 1 reference controland 2 Blank controls Temperature: 14.7-16.80C Total Suspended Solids and pH forsludge on day of testing: TotalSuspended Solids= 4.9 g/L (mixed liquor).Finaltestsolution mixed liquorsuspended solids= 1.92g/L. pH oftheactivated sludge mixed liquorgave an initiraeladingof6.4and a finalreading of 6.7. Element Basis: Respirationinhibitioans determined by oxygen consumption. MethodRemarks:Dissolvoexdygeninthetesstolutiownass quitleow duringthe30-minuteexposureportionofthetest.The technicianrealized thetestreactorswere notbeingsufficientaleyratedafterthe30-minute readingsand adjustedtheairflow.Because the3-hourdata isthemost commonly used data,itwas decidednotto re4estdue tothisoccurrence. Test temperatureswere notcontrolledduringthetest.The respiration rateswere normalizedto200C forthecalculations. RESULTS Nominal concentrations:Two Blankcontrolso,ne referencesubstance (10mg/L 3,5-DCP),two 1000 mg/L testmatedalsolutions. AnalyticalMethodology: Analysisof DO concentrationisnalltest solutionswere performedusinga dissolvedoxygen meter. Control response: Satisfactory Remarks: Values reportedareforthetestsample. No calculationwsere made toadjustfortheconcentratioonfthetestsubstanceinthetest sample. CONCLUSIONS The testsample containing24.5% PerfluorooctanesulfonaLtiet,hiumsalt exhibiteda maximum inhibitoerfyfectof35% upon respiratioant a nominaltestsubstanceconcentratioonf 1000 mg/L at3 hours exposure. The 3-hourEC50 (respiratiionnhibitioins)>1 000 mg/L. Submitter: 3M Company, EnvironmentalLaboratoryP,.O.Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Thisstudylacksinformatioonn the sample puritya,nd thetestwas conductedattemperaturesbelow those recommended by themethod. Oxygen concentrationaslsowere toolow to allowan adequate measurement of oxygen uptakeratefollowintghe30minuteexposure portion.These 30-minuteresultwsere considered invalifdorthestudy.Itwas concludedthat,sincethe 3-hourresultshow acceptabletoxicittyh,e30-minuteresultsalmostcertainlwyould have shown thesame. REFERENCES This studywas conducted by the 3M Company, Environmental Laboratory,Lab Request number Ml 018, 2/22/94. OTHER Last changed: 5/3/00 EVALUATION OF FC-94-XBY OECD ACTIVATEDSLUDGE RESPIRATION INHIBITIOTNEST #209 Results ,TheinhibiteofrfyecotfFC-94-Xt,helithisuamlotfperfluorooctanesulofnonate, activatseldudgerespiratwiaosnevaluateudsingOECD Test# 209.After calculatteedmperatucroerrectiomnesa,surementfsollowian3g-hours exposurteoFX-94-Xat1000mg/L,showedactivatseldudgeoxygenuptake ratewsereinhibitbeyd34%.Thereferenscuebstanc3e,,5-dichlorophaetnol, 10mg/Lcausedaninhibitoifo5n6%. Discussion, Thoughthistudyhaddeficitthse,analysctonsidetrhsatheresulatrse sufficiteonatssessthepotentifaolrwastewatetreatmesnytsteminhibitbiyon FC-94-X. One studydeficwiatsthatoxygenconcentratiwoenrsetoolowtoallowan ade.quatmeasuremenotfoxygenuptakeratefollowitnhge30-minuteexposurpeortionftheOECD #209studyT.hese30-minutreesulatrsethus invalainddshouldbeignoredT.he 30-minutresultasrenotcrititcoatlhe usefulneosfsthestudyh,owever,becauseinhibitiisaolnmostalwaysgreater followi3n-ghourosfexposureI.fthe3-houresulsthsowacceptabtloexicity, the30-minutreesultaslmostcertainwloyuldhaveshownthesame. A seconddeficiisthatemperatucroentrwoals notused.Measuredtest temperaturweesrelowerthanthatrecommendedbg'thmeethodbyasmuch as 5.5OC.However,altlestwsereconductewdithi1n.5degreesofeachother. Also,priotrocomparisoonftheoxygenuptakeratesc,alculatiwoenrsemade toadjusttheratetsothoseliketloyhaveoccurreadt20 OC. Theadjustment usedwas theruleofthumbthatmetabolircateosfmesophilmiiccrobial populatiotnyspicaldloyubleforeach10OC Increasientemperature. July22,1998 ,JNTERNAL CORRE,SPONDENCE13M TO: ERIC A. REINER FROM: SUSAN A. BEACH SUBJECT: Review ofOECD 209 and BOD/COD TestResultsforFC-94-X DATE: July8,1998 Ihave reviewedthe resultfsromthetestof FC-94-X on theinhibitiofnactivatesdludgerespiratioinhibition (OECD 209) and have a few comments. 1 . Althoughthe dissolvedoxygen inthetestsolutionwsas quitelow duringthe30-minuteexposure portionofthe test,itwas adequate after3-hoursexposure.The techniciarnealizetdhetestreactorswere notbeing sufficientaleyratedafterthe30-minutoreadings,and adjustedtheairflow.Because the3-hourdataisthe most commonly used data,itwas decidednottore-.tedsute tothisoccurrence. 2. At the timethistestwas run,thelaboratordyidnothave a procedureinplaceforcontrollitnhgetemperature duringexposure. The temperatureofthetestreactorsfluctuatewdiththetemperatureinthe hood. Because thiswas testedinFebruaryinMinnesota,itisnotsurprisintghatthetesttemperaturewas so low. The respiratiornateswere normalizedto2(rC forthecalculations. 3. The softwareused toproducethe datareductiosnheetsdidnot have an easy way todepictexponents,so the equationforthetemperature-adjusterdespiratiroantes(Ra)isdiffictuoltunderstand.Itisas follows: Ra = R adjustedto2CPC R x Where 0 = 1.072 x = T (observed)-200C if 4. Even though the 30-minutedataisinquestionand thetestwas conductedatlowerthannormaltemperatures, the data are usefulforthe interpretatioofnthetoxicitoyfFC-94-X toactivatesdludge.An average of34% inhibitiwoans seen at1000 mg/L. We generalldyo notconsiderinhibitiloenssthanor equalto 15% to be significawnhten usingthisprocedum due tothe inherentvariabiliotfythe method. As forthe BOD/COD testingw,e have been unabletolocatethe dataatthistime. Pleasesee me ifyou have any questions. Regards, Susan A. Beach ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MD(F-DUQUOR LR Date ;?t@t;)Ily Analyst Page I of 14 Type Test: OECD ACTIVATED SLUDGE RESPIRATION INHIBITION, 209 ACTIVATED SLUDGE MIXED LIQUOR Date Obtained Date Used Mixed Uquor Suspended Solids(MLSS) g/L MLSS infinaltestsolution g/L InitipaHl (p,q (Isttestsoin.prepared) Comments Adjusted'to FinalpH (o, g/L (lasstoln.prepared) TEST SUBSTANC Sample DescriptionFC-:-CJLI-Z InitiaplH of highestconc. tested Comments -7@.R AdjustedtopH REFERENCE SUBSTAN Sample Description %C\ALLRCEZ:beApl- InitipaHl of stocksolutio(n';;00 mg/L)- Comments 'Adjustedto pH EXPOSURE CONDMONS Test Duration 'V@a3@?-S Test Temperature Range: 14. Comments t,, c LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIONTEST #209 TEST SOLUTIONS PREPARATION Test Substance: FC- - 9 Lt Sample Description: Analyst: Date: ,7@ o%-@- LA Test Conc. mg added matl 12er500 mL mL MO(l) water per 500 mL mL syn.sewage feed 2er 500 mL L mL microbial inaculum oer 500 mL TotalVolume mL /COO m Zq -ZjzAfz@i/gy COA)CITc--,T- )AIJriAL Sol,, @ilu tocx) (1) MilliporMeilli-ORhigh puritywater ENVIRONMENTAL LAIBORATCRY OE",D ACTIVATED SLUDGE FiEc-PIRATION INHIEITICN TEST 9209 RAW DATA Tes,elSubs-,,ance: 9 4-X Source of Ac#4,ivaStie'dudgeMixedUquor:hit, Ul,-,TaE@-keTreqir,,Ps,,li DateRec--zived: I-CIL( DateUsed: Analys, 30-,MINUTE EXPOSURE TestSubs=ce Concentration O@it DISSOLVED OXYC-@r-NCONC=Nrir--.A-i]CNS, mg(L TIME-MINUTES 1.0 2.0 2.5 3.0 3.5 4.0 4.5 S.o Temp cc F-KOCNTRCL I q.0 b,ci 110.9 1r Io.<,-I - I- I I SK CCNTRCL 11 9.0 1-7,-717-q 1-7.1 (@,g S"9 3-HOUR EXPOSURE Dr,-Z-@OLNr=-ODXYC-r--N CCNC-=NTr--A-iICNS, mgtl Ie--tSubs=ce Concentration m-KCCNTRCL I /6-C TIME - MINUTES Temo 0.5 1.0 1.5 2.0 2.5 13.0 3.S 4.0 4.5 5.0 =.S cc I I I I I (o i7,V -7,1 -3 G-0 6,3 5-,!g15,3 15.1 1q,g lq,5- 1( 15.3 S-..l11419 q. 114I.r lq,3 Iq.1 13@@ 3.+ 135- 13)3 I- -=K C*--dNTRCL 11 ,:,-Hof ac*.ivated sludge mixed liquorcH Of highest concentrationtes,,ed: @est temperature: Initial Gq Initialti, S.-7 F:;nai(@o, F'nal t.,S' Z4.(10 Li,g ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION CALCULATION OF RESLA.TS INHIBMON TEST #209 Test Substance: FC-94-x Analystf:M 30-Mlnuto Ex)osure Dissolvd OxyCen Concentrati s,mg/L /L 0.6 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5..0O0@ 5.5 BK1 7.0 6.9 6.4 6.0 5.7 5.4 5.0 4.7 4.3 @ 4.0 3.6 looo.o 2.3 1.7 1.5 1.2 1.0 1 0.8 0.5 1 1 iooo.o 1.2 0.0 0.6 0.5 T -C R (1 16.6 0.707 16.8 0.474 16.8 0.400 BK2 8.0 7.7 7.4 7.1 % diffincontrols(4): 10.1 6. 1 6.5 6.2 5.9 1 5.6 EC50 (96% Cl):> 1000 mgtl 5.4 J_ 5.1 3-Hour Exposure Dissolv d Oxycen Concentrati s, m cov/ /L .0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.6 5.0 5.5 BK1 7.9 7.6 7.4 7.1 6.8 6.5 6.313 6.0 5.7 5.4 5.1 iooo.o 1 6.6 6.3 6.0 5.9 ;5.7 5.5 5.33 5.1 1 4.9 4.7 1 4.5 iooo.o 5.3 5.1 4.9 4.7 4.5 4.3 4.1 53.9 3.7 3.5 3.3 15.2 Tamp. OC 14.7 16.0 15.9 0.580 n (1) 0.558 0.391 0.400 BK2 1 7.4 1 6.9 6.5 6.2 6.0 5.7774 5..40019-1 5.2 4.9 1 4.6 % diffin controls(4): 2.3 EC 60 (95% Cl):> 1000 mci/L 14.9 (1) Ro Oxygen uptake rate(mg 02&-ndn) a- 1.072 (2) Ra- R adimied to IRI@." R&Cm- mean ad*ted R. mdmb (3)% lnldbkki)(mRaCm)-RmS/(RaCm)lloo Bk-blanckontrSonls.ample (4)% dnioreinncceortroIlIsR:&Bkl-RaBk2VRa(CTmwlocloonotrmoulsstbewkhl1n6%oleacohther.)Tobs-samplteomp (6)MeanadjudeRd.coriro[bR=aBkl+RaBl2y2 x-Tobs-20*C 0.553 ENVIRNOMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST#209 TEST SOLUTIONS PREPARATX)N Test Substance: Sample Description: @RoA Analyst: I L-nbp\ tc, i-,Ajo Date: Stock Solution Preparation::- IL- A scn, A b-oM --r@ U\JrFn -@n_ k-D -Li ri-7ft 0%_@H -7,q L MQ u:bTE(Z or-3. Test Conc. mg/L mL added 12er500 mL mL MO(l) water 22r 500 mL mL syn. sewage feed 12er500 mL mL microbial inoculum per 500 mL TotalVolume mL ID -7Li (1) MilliparMeilli-ORhigh puritywater ENVIRONMENTAL LABORATORY OE--D AC-nVATE-:) SLUDGE RE-FIRAI-ION INHIBITION TEST -209 RAW DA i-A Tes*L Substance: Source of Ac#*IvatedSi'udge Mixed Liquor: Date Re---cived: L( Analys, @@l + T- ST, Pl Date Used: 30-,MINUTE EXPOSURE Test Substance ConcentrationQ%jtL) 0.5 1.0 F,KCONTRCJ- I I- 1 DISIZCLVED OXYC-=-.NCONC=NTRA-iICNS, mg(L TIME-,MINUTES 1.5 2.0 2.5 3.0 3.5 -4.0 4.5 5.0 Temp S.c CIC 5;q 14,-@-14.3 1L40. -7,7 -7,9- 1-7,3 17, cZ 17.6 . ...................... '3.(o1/(Olb EK CCNI FICL 9 1 3-HOUR EXPOSURE ENSSOLVE-:) OXYC-=N CCNC=NTm-A-iICNS, mgl- Test Substance TIME - MINUTES Temo ConcenWation (MI/L 0.5 1.0 1.5 2.0 2.5 13.0 3.5 4.0 4.5 C.0 5.5 SK CONTRCL I ,-1.9 1,6 7,q -7,1 1(o.,? (0, (o,C)!5, I!&,). 31 1 3@D 7,cl 1-1,1 @7. -1 1-7,S-, q -7.3 -7.R '110 liq,or- P-K C'-.N-Md C.L U 7,q [-7,J P-oHof acoivatedsludge mixed liquor: Initial *pH of highestconcentrationtested: Initial (a Test temperature: Final F-nal (@o--7- EWIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRA71ON INHIBMON TEST #209 CALCLLA11ON OF RESULTS Test Substance: 3.5-Dichlorol2henRoelferenceSubstance Analystf:lff 30-Minuto Ex osure mg/L 0.5 1.0 1.5 n BKI 7.0 I 6.9 6.4 10.0 8.6 8.4 8.3 Dissol d Oxycon Concentratis,m 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 F.. 6.0 -5.7 5.4 5.0 4.7 4.3 4.0', 3.6 8.1 7.9 7.7 7.5 7.3 7.2 7.0 6.8 To -c R (1) 16.6 0.707 14.7 0.363 7.7 7.4 7.1 1 6.8 6.5 6.2 6.9 5.6 1 5.4 5.1 % diffincontrol(s4): 10.1 ECSO (95% Cl):> 10 mgll 3-Hour Exposure Dissolwdv Oxyten Concentratis,mg/ /L .0.5 1.0 1.5 2.0 2.5 3.0 1 3.5 4.0 4.5 5.0 5.5 BK1 7.9 7.6 7.4 7.1 6.8 6.5 6.3 6.0 5.7 5.4 5.1 10.0 8.2 8.1 8.0 7.9 ,7.8 7.7 7.5 7.4 7.3 7.2 7.0 15.2 Tww. oc 14.7 14.5 0.580 R (i 0.558 0.241 BK 2 7.4 7.1 6.9 6.5 6.2 6. 1 5.4 5.2 4.9 4.6 % diffincontrol(s4):. 2.3 EC 50 (95% Cl):< 10 mgll 14.9 1 .553C (1)R- Oxygenqmam rde (mg02&-Wn) (2)Ra- R acQM*d So20-C- Pe.Exi (a)% inhn*bn-i(R&Cm).Ras/(Racm)lioo (4)% dn[wonceIncoraralsI:lRaBkl-RaBk2MaCm]100(Twomdmb (5)Mom adoged R.wi*obu PUMI+RaBk2y2 mustbe whmn 16% ofeachothw.) 1.072 R&Cnv- mean adlmlodR,mdrols Bloxblanmkdrol,Sm umple Tobsu sampletamp xn Tobs-2010