Document zQk4wpM6de1dKOQ0dx5jqbYan
Appendix to TNO-report of 3M-02
Final version
CONFIDENTIAL
04-12-2006
APPENDIX TO TNO-REPORT OF STUDY 3M-02
Project / study number: 031.10074 / 3M-02
TNO Quality of Life
TNO Biosciences
Gaubius laboratory Zemikedreef 9. P.O. Box 2215 2301 CE Leiden The Netherlands Phone +31 71 518 1209 Fax +31 71 518 1901
Authors: L H. Cohen
E.J. Pieterman E Hoegee-de Nobel
In assignment of: 3M. Medical Department
Study monitor:
John L. Butenhoff, Ph.D.. CIH. DABT
Corporate Scientist
Medical Department
3M Center, Building 0220-02-E-02
St.Paul, Minnesota 55144-1000
USA
Phone: +1.651.733.1962
Fax:
+1.651.733.1773
E-mail: ilbutenhoff@mmm.com
Study director: Dr. Louis H. Cohen
Status and Date:
Final version: 04 December 2006
Previous versions: Draftl: 18 October 2006 Draft2: 30 October 2006
Number of pages: 172
Authorization by Dr. P.H.A. Quax, Technology manager of Vascular and Metabolic Diseases:
Page A1 of A172
Appendix to TNO-report of 3M-02
Contents Contents
Final version
04-12-2006
page A02
A. Code E3L mice before and after matching
A03
B. Bodyweight and food intake measurements B1. Individual bodyweights B2. Food intake measurements
A04 A04 A07
C. Plasma measurements C1. Plasma cholesterol measurements C2. Plasma triglyceride measurements C3. Pooled lipoprotein profile measurements C4. Pooled plasma ALAT measurements C5. Plasma ketone bodies measurements
A13 A13 A15 A17 A24 A25
D. Measurements in metabolic cages D1. Code mice and metabolic cages D2. Average bodyweight mice in metaboliccages D3. Food and drink intake measurements D4. X activity measurements D5. O2 consumption,CO2 production and respiratory exchange ratio measurements D6. Energy expenditure
A28 A28 A28 A29 A38
A41 A50
E. Fecal bile acid, sterol and fatty acid measurements
A53
E1. Fecal weight measurements, bodyweight and food intake
Measurements
A53
E2. Fecal bile acid measurements
A60
E3. Fecal sterol measurements
A83
E4. Fecal fatty acid measurements
A107
F. Intestinal cholesterol absorption
A122
G. Liver weight, perigonadal fat weight and lipidcontents liver G1. Liver weight and perigonadal fat weight G2. Lipid contents liver
A127 A127 A129
H. HMG-CoA reductase and cholesterol-7a-hydroxylase activity
in liver microsomes and sterol-27-hydroxylase activity in liver
mitochondria
A136
H1. HMG-CoA reductase and cholesterol-7a-hydroxylase activity
in liver microsomes
A136
H2. Sterol-27-hydroxylase activity in liver mitochondria
A148
I. Measurements in vitro experiment (rat hepatocytes)
A156
Page A2 of A172
Appendix to TNO-report of 3M-02
Final version
Appendix A. Code E3L mice before and after matching
1 mouse# cage# beforematching 658 86 663 86 712 93 716 93 752 98 758 98 763 99 784 102 790 102 791 102 641 84 645 84 646 84 674 88 737 96 738 96 740 96 760 99 765 99 785 102 650 85 678 88 690 90/91 699 90/91 729 95 754 98 756 98 759 98 766 99 793 103 681 89 686 89 704 92 705 92 706 92 710 92 768 100 771 100 792 103 795 103 665 87 666 87 667 87 671 87 679 88 700 90/91 730 95 731 95 744 97 799 103 714 93 721 94 727 94 769 100 774 100 776 101 778 101 782 101 783 101 797 103 652 85 657 86 670 87 680 89 687 89 696 90/91 720 94 724 94 761 99 762 99
excluded(655) 85 excluded(656) 86 excluded(741) 96 excluded(742) 96 excluded(748) 97
eartag LR 01 12 00 20 00 02 11 00 02 12 10 21 02 01 10 01 20 00 21 10 01 02 01 11 10 01 20 12 02 10 10 02 00 10 01 02 00 11 00 11 10 01 11 12 12 20 01 11 00 12 01 10 12 10 02 00 01 02 22 21 20 10 02 00 12 00 00 20 10 01 12 00 21 02 20
group# mouse# 1 cage#
after matching
11
1
11 2
12
3
12
4
13
5
13
6
14
7
15
8
15
9
1 5 10
2 6 11
2 6 12
2 6 13
2 7 14
2 8 15
2 8 16
2 8 17
2 9 18
2 9 19
2 10 20
3 11 21
3 12 22
3 13 23
3 13 24
3 14 25
3 15 26
3 15 27
3 15 28
3 16 29
3 17 30
4 18 31
4 18 32
4 19 33
4 19 34
4 19 35
4 19 36
4 20 37
4 20 38
4 21 39
4 21 40
5 22 41
5 22 42
5 22 43
5 22 44
5 23 45
5 24 46
5 25 47
5 25 48
5 26 49
5 27 50
6 28 51
6 29 52
6 29 53
6 30 54
6 30 55
6 31 56
6 31 57
6 31 58
6 31 59
6 32 60
7 33 61
7 34 62
7 35 63
7 36 64
7 36 65
7 37 66
7 38 67
7 38 68
7 39 69
7 39 70
I
04-12-2006
Page A3 of A172
Appendix to TNO-report of 3M-02
Final version
Appendix B. Bodyweight and food intake measurements
B1. Individual bodyweights
G roup
M ouse#
cage#
r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in r u n in AVERAGE
SD r u n in r u n in r u n in ru n in r u n in
641 645 646 650 652 657 658 663 665 666 667 670 671 674 678 679 680 681 686 687 690 696 699 700 704 705 706 710 712 714 716 720 721 724 727 729 730 731 737 738 740 744 752 754 756 758 759 760 761 762 763 765 766 768 769 771 774 776 778 782 783 784 785 790 791 792 793 795 797 799
84 84 84 85 85 86 86 86 87 87 87 87 87 88 88 88 89 89 89 89 90/91 90/91 90/91 90/91 92 92 92 92 93 93 93 94 94 94 94 95 95 95 96 96 96 97 98 98 98 98 98 99 99 99 99 99 99 100 100 100 100 101 101 101 101 102 102 102 102 103 103 103 103 103
exclu d e d (655) exclu d e d (656) exclu d e d (741) e x c 1u d e d (7 4 2 ) exclu d e d (748)
85 86 96 96 97
e a rta g LR 10 21 02 01 20 10 01 12 10 01 11 02 12 01 02 12 00 10 02 12 01 00 11 20 00 10 01 02 00 01 20 00 10 20 12 10 01 11 10 01 20 00 00 01 20 02 12 00 10 01 11 21 02 00 10 11 02 00 01 02 22 00 10 02 12 00 10 11 21 12
12 00 21 02 20
b o d yw e ig h t (g)
w e e k -2 w e e k -1
week 0
3 0 .0
30.1
2 9 .2
2 7 .5
28.1
2 8 .7
3 1 .2
2 7 .5 -- 299--
3 0 .9
2 7 .6 3 0 .0
3 0 .6
2 8 .5 311
3 1 .4 3 2 .6
3 0 .9 3 1 .8
3 2 .2 3 1 .9
30.1
3 0 .9
31.1
3 0 .5 2 5 .0
3 0 .7 2 5 .6
3 1 .3 2 5 .4
2 8 .0 3 0 .7
2 7 .7 3 1 .0
2 7 .9 3 1 .3
2 8 .6
2 7 .9
2 9 .9
3 1 .5 2 9 .9
3 1 .4 3 0 .0
3 1 .9 3 0 .3
2 9 .3 3 1 .4
2 9 .0 3 1 .5
3 0 .9 3 1 .5
2 9 .4
2 9 .2
3 0 .9
29.1 2 9 .9
2 8 .9 2 9 .6
29.1 3 1 .5
3 1 .4 2 6 .2
3 1 .0 2 6 .3
3 1 .8 2 6 .7
2 7 .6
2 7 .9
2 7 .8
2 9 .7 2 9 .0
2 9 .4 2 9 .7
2 9 .5 2 9 .2
3 1 .4 3 2 .0
3 0 .9 3 1 .9
3 0 .4 3 1 .8
2 4 .6 2 9 .4
2 4 .8 29.1
2 6 .4 2 9 .8
2 9 .0
2 9 .6
3 0 .2
2 8 .8 2 8 .2
2 8 .4 2 8 .8
2 8 .9 29.1
3 2 .5 2 7 .5
3 2 .6 27.1
3 1 .8 2 9 .0
2 6 .5
2 6 .9
2 8 .0
2 5 .0 2 9 .4
2 5 .3 2 9 .4
2 7 .0 3 0 .7
2 8 .8 2 8 .9
2 8 .5 2 9 .0
2 9 .3 3 0 .0
28.1
2 8 .7
2 8 .3
3 0 .6 2 7 .0
3 0 .9 2 7 .4
3 0 .9 2 7 .7
2 9 .0 2 8 .8
2 9 .4 2 9 .0
3 0 .5 31.1
2 8 .2
2 8 .0
2 9 .0
2 7 .8 2 4 .5
2 7 .3 2 4 .7
2 9 .3 26.1
2 5 .2 23.1
2 5 .7 2 3 .8
2 6 .4 2 4 .0
2 5 .5
2 5 .9
2 5 .5
2 5 .4 2 7 .2
2 5 .0 2 6 .8
2 5 .9 2 6 .5
2 8 .0 2 7 .0
28.1 2 7 .6
2 7 .9 2 8 .9
28.1
2 8 .2
3 0 .4
2 9 .0 2 7 .2
2 9 .3 27.1
2 9 .8 2 8 .0
2 5 .5 2 8 .2
2 5 .7 2 8 .3
2 5 .3 28.1
2 7 .5
2 7 .6
2 9 .0
27.1 2 8 .6
2 7 .0 2 8 .9
2 9 .5 2 8 .9
2 9 .3 2 8 .8
2 9 .7 2 8 .7
2 8 .8 2 9 .0
26.1 2 7 .4
2 6 .8 2 7 .5
2 6 .6 2 6 .0
2 7 .7
2 7 .8
2 7 .9
2 6 .8 2 9 .0 29.1
2 6 .7 2 9 .5 2 9 .4
2 6 .9 2 8 .0 2 9 .5
2 8 .4 3
28.51
2 9 .0 3
2 .0 3
1 .9 3
1 .9 3
2 7 .3
2 7 .0
2 8 .5
2 2 .2
2 2 .9
2 4 .6
2 9 .0 3 1 .0
2 9 .2 3 1 .6
3 0 .9 3 2 .2
3 0 .6
3 0 .9
3 1 .0
04-12-2006
Page A4 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group Mouse# cage#
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD
4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
1 1 2 2 3 3 4 5 5 5
6 6 6 7 8 8 8 9 9 10
11 12 13 13 14 15 15 15 16 17
18 18 19 19 19 19 20 20 21 21
22 22 22 22 23 24 25 25 26 27
28 29 29 30 30 31 31 31 31 32
33 34 35 36 36 37 38 38 39 39
eartag LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
week 0
31.9 31.1 29.8 28.9 30.5 29.3 25.9 28.9 29.0 26.6
29.19 1 .8 5 29.2 28.7 30.6 31.9 30.0 28.3 30.9 26.4 26.5 28.8 29.13 1 .8 0
28.5 30.3 31.8 27.8 27.0 31.1 29.0 26.1 27.9 27.9
28.74 1 .8 2
30.9 29.1 29.2 30.4 31.8 26.4 28.9 29.8 26.0 26.9 28.94 1 .9 5 31.3 25.4 27.9 29.9 30.9 29.5 30.7 29.3 27.7 29.5
29.21 1 .7 8
30.2 31.8 28.0 30.4 28.0 25.3 28.1 29.0 29.5 28.0
28.83 1 .7 9 31.1 32.2 31.3 31.5 31.5 26.7 29.1 29.0 24.0 25.5 29.19 2.88
week 1
31.4 29.8 30.2 29.4 30.0 28.4 25.8 29.7 28.9 26.2
28.98 1.76 29.4 28.5 30.7 31.7 30.2 28.2 31.6 25.9 26.3 28.1 29.06 2 .0 3
27.4 29.2 32.4 27.8 26.3 30.8 30.1 26.4 27.5 27.8
28.57 2 .0 0
30.5 29.3 28.6 31.0 31.6 25.6 28.3 30.3 27.5 26.1 28.88 2 .0 4 29.5 24.7 26.9 29.4 29.4 28.4 27.9 27.9 26.3 28.0
27.84 1.53
29.2 31.2 27.1 30.8 28.0 24.8 27.8 27.7 29.8 27.5
28.39 1.91 30.1 30.2 28.8 30.6 30.4 26.2 28.2 28.4 23.4 25.2 28.15 2 .4 6
week 2
32.6 29.8 29.8 28.6 30.9 27.8 24.8 29.0 29.8 26.3
28.94 2 .2 4 29.8 28.9 30.3 32.3 29.7 29.4 31.7 25.9 26.5 28.8 29.33 2 .0 0
27.3 29.7 32.0 28.6 26.1 30.0 29.9 25.3 27.8 27.4
28.41 2 .0 3
30.2 29.2 29.0 31.6 31.4 25.6 29.1 30.7 28.0 26.3 29.11 2 .0 2 25.6 23.8 26.8 25.4 26.9 26.7 28.3 25.8 24.0 26.1
25.94 1.36
28.8 31.7 27.6 30.9 28.4 24.9 28.1 28.4 30.4 27.2
28.64 1.98 27.0 26.6 26.6 28.9 28.4 25.0 26.6 26.0 22.4 23.6 26.11 2 .0 0
w eek 2.3
25.0 23.8 26.6 25.0 27.0 26.5 28.4 25.6 24.6 25.3 25.78 1.35
26.9 26.4 25.2 27.7 27.3 24.5 25.6 25.4 21.7 23.6 25.43 1.82
week 3
32.5 29.8 29.6 27.9 30.5 28.1 25.0 28.6 29.7 26.2
28.79 2.15 30.2 29.0 29.3 31.7 30.2 29.6 31.7 25.6 26.8 28.2 29.23 1 .9 6
27.1 28.9 32.6 28.8 25.6 29.1 29.9 24.9 27.8 27.3
28.20 2.20
29.7 29.2 28.9 30.8 31.1 25.7 28.3 30.2 27.4 27.0 28.83 1 .7 4 25.6 24.8 27.0 25.7 27.9 27.9 28.4 28.0 25.4 27.0
26.77 1 .3 0
28.7 31.4 27.1 30.5 28.1 25.1 27.7 28.2 30.0 27.1
28.39 1 .8 5 26.2 25.0 24.7 27.8 27.1 24.6 25.5 25.3 22.3 23.5 25.20 1.61
w eek 3.4
28.0 24.8 27.3 27.5 29.1 28.6 29.5 29.7 25.2 27.6 27.73 1.67
26.0 24.1 25.0 27.7 26.8 25.2 26.2 26.0 23.1 24.3 25.44 1.37
week 4
32.6 29.9 29.8 27.9 30.4 27.6 25.7 28.1 29.2 26.1
28.73 2 .0 8 31.0 28.9 29.4 31.9 30.2 29.4 31.8 26.4 27.0 28.3 29.43 1.87
27.8 29.0 32.5 29.2 25.2 29.2 30.0 25.0 28.9 26.7
28.35 2 .2 7
29.7 28.9 28.9 30.9 31.1 25.3 28.2 30.6 27.5 27.3 28.84 1.83 27.5 24.2 26.7 28.1 28.9 28.4 29.2 29.3 25.7 27.8
27.58 1.63
28.3 31.3 27.1 30.3 28.8 25.1 27.9 28.9 30.6 27.2
28.55 1.87 26.3 24.7 25.3 27.9 27.1 25.3 25.8 26.1 22.8 23.8 25.51 1.50
Bodyweight (g) w eek 5 w eek 5.3 w eek 5.6
32.9
33.9
33.9
30.5
31.2
30.5
30.7
30.4
31.0
28.7
28.1
28.2
31.1
31.1
30.6
29.6
29.5
29.4
26.5
26.7
27.2
30.0
30.4
30.6
30.5 27.1
30.6 27.2
31.0 27.3
29.76 1 .9 0
29.91 2.14
29.97 2 .0 2
31.4
33.0
33.1
29.4
29.8
30.0
29.9
31.2
32.0
32.9
32.8
33.1
31.0 30.2
30.8 30.1
31.0 29.8
32.9
32.8
32.8
26.2
26.5
26.6
26.6
26.9
26.6
28.6
29.0
28.8
29.91 2.31
30.29 2.33
30.38 2 .4 8
28.2
27.8
28.3
28.8
28.8
28.9
32.9
32.2
32.3
28.9
28.8
28.9
25.8
25.8
26.3
29.0
28.7
28.5
30.2
30.1
30.1
25.1
25.0
24.7
29.2 27.1
29.0 27.0
29.7 27.0
28.52 2.21
28.32 2.07
28.47 2 .1 2
30.2 29.3
30.3 29.3
30.2 29.2
28.8
29.2
28.9
30.4
30.9
30.7
30.7
31.0
30.7
25.3 27.3
25.6 27.0
25.8 26.7
30.2
30.2
30.2
27.1
27.7
27.2
27.5
27.7
27.7
28.68
28.89
28.73
1 .8 0
1 .8 2
1.78
28.6
28.9
30.0
25.0
25.2
25.3
27.4
27.6
27.8
28.2
28.5
28.1
29.2
28.6
29.7
28.2
28.5
28.6
30.3
30.3
30.1
29.3
29.3
29.9
26.5
27.3
27.2
28.0
27.7
28.7
28.07 1.51
28.19 1 .3 7
28.54 1.52
28.5
28.4
28.5
31.5 27.4
31.3 27.3
31.5 27.2
30.3
30.2
30.0
28.7
28.8
28.4
25.5
25.6
25.4
27.6 28.9
27.6 29.1
27.8 29.1
30.4 26.8
30.3 26.7
30.7 26.7
28.56
28.53
28.53
1 .8 2
1 .7 7
1.87
28.0
28.2
27.7
27.2 26.1
27.3 26.2
26.7 26.6
28.3
28.2
28.5
27.4
27.4
28.2
26.1
26.1
26.1
26.2
26.0
26.2
26.5 22.8
26.7 23.6
26.9 23.0
24.6
25.1
24.6
26.32
26.48
26.45
1 .6 4
1 .4 2
1.65
week 6
32.9 30.1 30.0 28.3 29.2 30.4 26.3 30.0 30.5 27.0
29.47 1.89 32.2 30.0 31.2 33.6 30.1 30.0 32.5 26.3 26.9 29.0 30.18 2 .3 4
28.4 29.2 32.3 29.2 26.1 28.8 29.8 25.1 29.4 27.6
28.59 2 .0 0
29.6 28.7 29.6 30.5 30.7 26.2 26.9 30.1 27.2 27.7 28.72 1.62 29.1 25.5 28.6 28.8 30.1 28.6 30.1 29.7 27.0 28.4
28.59 1.42
28.7 31.4 27.0 29.7 28.0 25.3 26.9 28.8 30.9 26.3
28.30 1.99 29.1 27.8 26.8 28.9 27.6 26.2 26.7 27.2 23.6 25.7 26.96 1.60
w eek 6.3
33.2 30.2 30.2 28.4 31.1 29.6 26.5 30.8 30.7 27.1
29.78 1 .9 9 32.8 30.0 31.8 33.1 30.3 30.9 32.7 25.9 26.5 29.1 30.31 2.53
29.1 29.2 32.5 29.3 26.4 29.1 30.2 25.4 29.6 27.8
28.86 1 .9 8
29.9 29.2 29.8 30.5 31.1 26.5 28.0 30.9 27.5 27.5 29.09 1.61 29.8 25.6 29.2 29.1 29.6 28.6 30.6 30.2 27.3 28.6
28.86 1 .4 8
28.7 32.1 27.4 30.2 28.5 25.6 27.2 28.6 30.6 26.6
28.55 1 .9 8 28.5 27.8 27.0 28.7 28.2 27.1 26.7 27.3 23.8 25.4 27.05 1 .5 0
week 7
33.5 30.6 30.5 28.5 30.8 29.8 27.0 30.9 31.0 27.6
30.02 1 .9 0 33.1 29.5 32.0 33.0 30.4 29.5 31.4 26.2 27.7 29.5 30.23 2.23
29.9 30.1 33.2 29.3 27.2 29.4 30.3 26.3 30.4 28.9
29.50 1 .8 7
29.4 29.4 30.0 30.5 30.7 26.4 27.1 30.7 27.9 28.3 29.04 1 .5 4 30.5 25.9 29.6 29.1 30.6 30.2 30.0 30.5 27.2 29.0
29.26 1 .5 7
28.9 32.0 27.6 30.0 28.1 25.5 26.7 28.5 30.4 26.3
28.40 2.01 28.6 28.4 26.8 28.6 28.3 27.2 26.9 27.1 24.0 25.2 27.11 1 .5 3
week 8
33.8 31.2 30.2 28.6 31.0 29.7 27.5 31.0 30.7 27.3
30.10 1.94 34.1 29.5 32.2 33.7 29.9 30.5 31.2 26.4 28.7 29.7 30.59 2 .3 2
30.2 30.0 33.1 29.5 27.0 30.1 30.1 26.4 30.8 28.6
29.58 1.91
29.7 29.0 30.2 30.3 30.8 26.5 27.6 30.9 28.1 28.5 29.16 1.47 30.4 25.6 29.1 29.1 30.4 29.9 30.4 30.6 27.7 29.1
29.23 1.56
28.8 32.1 26.8 30.3 28.5 25.6 26.7 28.3 30.1 26.5
28.37 2 .0 3 29.0 28.6 27.1 29.1 28.6 27.3 27.2 27.6 24.3 25.7 27.45 1.53
w eek 8.4
33.7 31.0 30.3 28.5 30.8 29.9 27.3 31.9 30.6 27.1
30.11 2 .0 3 33.7 29.5 33.1 33.5 30.8 31.0 31.7 26.5 28.9 29.5 30.82 2 .2 9
30.5 30.1 33.2 29.3 27.2 31.0 30.3 26.1 31.1 28.5
29.73 2 .0 5
29.0 28.7 29.8 30.4 30.7 26.4 27.2 30.6 27.6 28.4 28.88 1.50 30.4 25.4 29.1 29.2 30.1 29.8 30.7 30.5 28.1 28.9
29.22 1.57
28.5 32.1 26.7 28.1 27.4 25.5 26.8 28.2 30.2 26.3
27.98 1.96 29.4 28.7 27.8 29.5 28.6 27.4 27.5 27.6 24.7 26.3 27.75 1.45
week 9
34.5 31.1 30.0 28.4 31.2 30.0 27.7 33.1 30.4 27.0
30.34 2.32 34.8 30.1 33.6 33.6 31.2 31.2 32.4 27.8 29.8 30.1 31.46 2.14
30.6 30.0 33.4 29.4 27.2 31.4 30.7 26.3 31.6 28.1
29.87 2.17
28.4 28.1 29.6 30.2 30.9 26.4 27.0 30.4 27.2 28.6 28.68 1 .5 5 30.2 25.0 28.9 29.3 29.6 29.7 31.0 30.4 28.5 29.0
29.16 1 .6 4
28.4 32.2 26.6 27.8 27.1 25.7 26.7 27.9 30.0 26.3
27.87 1 .9 5 29.7 29.2 28.2 29.8 28.9 27.8 27.6 28.0 25.0 26.8 28.10 1 .4 5
1 w eek 10
34.3 31.4 30.5 28.9 32.0 29.3 28.0 33.2 31.7 27.4
30.67 2 .2 6 35.4 30.6 33.3 33.8 30.6 30.7 32.9 28.3 30.8 30.1 31.65 2 .1 2
31.0 30.9 33.8 28.4 27.3 30.9 31.3 25.9 32.7 27.7
29.99 2 .5 4
28.2 28.1 29.2 29.6 30.3 26.2 26.7 29.7 26.8 28.1 28.29 1.41 31.3 25.4 29.6 29.4 29.5 30.0 31.2 30.7 28.4 29.0
29.45 1.70
27.8 31.3 26.2 27.7 26.8 25.2 26.7 27.6 29.9 26.0
27.52 1.85 29.8 29.5 28.0 29.8 29.5 27.9 27.5 28.1 25.1 27.6 28.28 1.45
Page A5 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0 1 2 3 4 5 5.3 5.6 6 6.3 7 8 8.4 9 10
week
0 1 2 3 4 5 5.3 5.6 6 6.3 7 8 8.4 9 10
week
0 1 2 2.3 3 3.4 4 5 5.3 5.6 6 6.3 7 8 8.4 9 10
Bodyweight, p values Mann-Whitney U-test
group 1 vs 2 group 1 vs 3 group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn p nn p nn p nn p nn
p
nn
p
10 10 0.739 10 10 0.481 10 10 0.853 10 10 0.796 10 10 0.529 10 10 0.631
10 10 0.912 10 10 0.631 10 10 0.971 10 10 0.075 10 10 0.393 10 10 0.631
10 10 0.796 10 10 0.579 10 10 0.796 10 10 0.004 10 10 0.631 10 10 0.011
10 10 0.631 10 10 0.481 10 10 0.912 10 10 0.019 10 10 0.739 10 10 0.001
10 10 0.436 10 10 0.631 10 10 0.912 10 10 0.218 10 10 0.912 10 10 0.002
10 10 0.971 10 10 0.143 10 10 0.247 10 10 0.029 10 10 0.165 10 10 0.001
10 10 0.739 10 10 0.105 10 10 0.218 10 10 0.063 10 10 0.143 10 10 0.002
10 10 0.739 10 10 0.123 10 10 0.143 10 10 0.075 10 10 0.165 10 10 <0.001
10 10 0.579 10 10 0.190 10 10 0.481 10 10 0.218 10 10 0.218 10 10 0.005
10 10 0.579 10 10 0.165 10 10 0.481 10 10 0.190 10 10 0.247 10 10 0.007
10 10 0.971 10 10 0.280 10 10 0.165 10 10 0.190 10 10 0.075 10 10 0.003
10 10 0.739 10 10 0.353 10 10 0.247 10 10 0.280 10 10 0.063 10 10 0.004
10 10 0.529 10 10 0.684 10 10 0.143 10 10 0.247 10 10 0.029 10 10 0.023
10 10 0.247 10 10 0.853 10 10 0.105 10 10 0.247 10 10 0.019 10 10 0.019
10 10 0.393 10 10 0.481 10 10 0.029 10 10 0.315 10 10 0.003 10 10 0.035
group 2 vs 4 group 2 vs 5 group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
n n p n n p n n p n n p__ n n
p
nn
p
10 10 0.912 10 10 0.796 10 10 0.579 10 10 0.579 10 10 0.739 10 10 0.529
10 10 0.853 10 10 0.143 10 10 0.353 10 10 0.481 10 10 0.631 10 10 0.853
10 10 0.796 10 10 0.001 10 10 0.353 10 10 0.005 10 10 0.393 10 10 0.007
10 10 0.684 10 10 0.005 10 10 0.280 10 10 <0.001 10 10 0.353 10 10 0.105
10 10 0.529 10 10 0.029 10 10 0.353 10 10 <0.001 10 10 0.684 10 10 0.393
10 10 0.280 10 10 0.043 10 10 0.247 10 10 0.001 10 10 0.631 10 10 0.631
10 10 0.247 10 10 0.035 10 10 0.123 10 10 0.001 10 10 0.393 10 10 0.796
10 10 0.190 10 10 0.075 10 10 0.105 10 10 0.002 10 10 0.579 10 10 0.912
10 10 0.165 10 10 0.089 10 10 0.075 10 10 0.004 10 10 0.684 10 10 1.000
10 10 0.218 10 10 0.075 10 10 0.105 10 10 0.009 10 10 0.579 10 10 0.912
10 10 0.218 10 10 0.039 10 10 0.089 10 10 0.003 10 10 0.912 10 10 0.853
10 10 0.165 10 10 0.190 10 10 0.052 10 10 0.002 10 10 0.853 10 10 0.853
10 10 0.035 10 10 0.075 10 10 0.011 10 10 0.002 10 10 0.315 10 10 0.436
10 10 0.009 10 10 0.015 10 10 0.002 10 10 <0.001 10 10 0.218 10 10 0.353
10 10 <0.001 10 10 0.035 10 10 <0.001 10 10 <0.001 10 10 0.105 10 10 0.579
group 3 vs 7 group 4 vs 5 group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn p nn p nn p nn p nn
p
nn
p
10 10 0.481 10 10 0.631 10 10 0.796 10 10 0.631 10 10 0.684 10 10 0.631
10 10 0.971 10 10 0.280 10 10 0.529 10 10 0.529 10 10 0.684 10 10 0.393
10 10 0.023 10 10 0.002 10 10 0.529 10 10 0.007 10 10 0.002 10 10 0.796
1 1 1 1 1 1 1 10 10 0.853
10 10 0.003 10 10 0.009 10 10 0.579 10 10 <0.001 10 10 0.043 10 10 0.023
1 1 1 1 1 1 1 10 10 0.007
10 10 0.011 10 10 0.165 10 10 0.579 10 10 0.001 10 10 0.353 10 10 0.011
10 10 0.029 10 10 0.393 10 10 0.912 10 10 0.011 10 10 0.579 10 10 0.015
10 10 0.043 10 10 0.315 10 10 0.529 10 10 0.009 10 10 0.796 10 10 0.007
10 10 0.015 10 10 0.579 10 10 0.796 10 10 0.015 10 10 0.912 10 10 0.007
10 10 0.052 10 10 0.796 10 10 0.739 10 10 0.035 10 10 0.631 10 10 0.035
10 10 0.023 10 10 0.631 10 10 0.481 10 10 0.019 10 10 0.529 10 10 0.005
10 10 0.003 10 10 0.853 10 10 0.353 10 10 0.029 10 10 0.190 10 10 0.003
10 10 0.019 10 10 0.739 10 10 0.315 10 10 0.035 10 10 0.165 10 10 0.005
10 10 0.043 10 10 0.529 10 10 0.165 10 10 0.143 10 10 0.089 10 10 0.019
10 10 0.052 10 10 0.353 10 10 0.165 10 10 0.481 10 10 0.063 10 10 0.063
10 10 0.123 10 10 0.089 10 10 0.190 10 10 0.912 10 10 0.035 10 10 0.075
group 2 vs 3 nn p 10 10 0.529 10 10 0.481 10 10 0.353 10 10 0.190 10 10 0.247 10 10 0.143 10 10 0.052 10 10 0.089 10 10 0.075 10 10 0.105 10 10 0.393 10 10 0.436 10 10 0.280 10 10 0.165 10 10 0.436 group 3 vs 6 nn p 10 10 0.579 10 10 0.912 10 10 0.796 10 10 0.912 10 10 0.971 10 10 0.971 10 10 0.796 10 10 0.971 10 10 0.684 10 10 0.579 10 10 0.218 10 10 0.218 10 10 0.063 10 10 0.052 10 10 0.043 group 6 vs 7 nn p 10 10 0.481 10 10 0.912 10 10 0.015
10 10 0.001
10 10 0.002 10 10 0.009 10 10 0.015 10 10 0.019 10 10 0.190 10 10 0.105 10 10 0.247 10 10 0.631 10 10 0.853 10 10 0.436 10 10 0.218
I
I P<0 0 5 I
Page A6 of A172
Appendix to TNO-report of 3M-02 B2. Food intake measurements
Final version
04-12-2006
cage#
84 85 86 87 88 89 90/91 92 93 94 95 96 97 98 99 100 101 102 103
number of Food on cage Food off cage mice week -2 (g) week -1 (g)
3 86.1
37.1
3 88.8
41.6
4 117.2 49.1
5 131.5 51.8
3 84.2
22.9
4 105.6 29.3
4 108.5 40.9
4 107.7 49.6
3 79.3
21
4 104.1
40
3 82.6
25.7
5 132.8
40
2 54.5
20.8
5 139.5 57.2
6 156.4 78.2
4 105.8 30.5
4 102.2 40.3
4 102.3 44.7
5 132.2
53.6
days
6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6
Food intake (g/day/mouse)
2.72 2.62 2.84 2.66 3.41 3.18 2.82 2.42 3.24 2.67 3.16 3.09 2.81 2.74 2.17 3.14 2.58 2.40 2.62
2.80 0.32
Food on cage Food off cage week -1 (g) week 0 (g)
82 25
81.8 19.7
108.9
30.3
132.7
39.4
77.6 10.4
103.9
20.9
105.2
27.7
108.2
40.3
80.3 16.7
108.8
31.1
81.6 17.1
130.8
28.5
52.2 9.5
129.7
33.2
152.1
51.8
104.2
20.5
106.9
31
106.8
33.2
130.6
41.7
days
7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7
Food intake (g/day/mouse)
2.71 2.96 2.81 2.67 3.20 2.96 2.77 2.43 3.03 2.78 3.07 2.92 3.05 2.76 2.39 2.99 2.71 2.63 2.54
2.81 0.22
Page A7 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A8 of A172
Group
1 1 1 1 1 AVERAGE SD 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 AVERAGE SD
4 4 4 AVERAGE SD 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 AVERAGE SD
cage#
1 2 3 4 5
number of m ice 2 2 2 1 3
Food on cage week 0 (g) 70.8 69 62.5 31 86.1
Food off cage w e e k 1 (g) 34.8 26.5 26.4 12.5 38.3
days
6 6 6 6 6
63 71 83 92 10 1
85.8 38.3 89.4 59.8 34.1
34.1 15.3 31.4 28.6 18.2
6 6 6 6 6
11 1 12 1 13 2 14 1 15 3 16 1 17 1
34.4 35 63 32.2 84.1 29 30.9
17 16.4 24.3 13.5 31.3 12.6 12.2
6 6 6 6 6 6 6
18 2 19 4 20 2 21 2
62.4 110.6 54.5 65.1
20.9 47.6 12.5 29.8
6 6 6 6
22 4 23 1 24 1 25 2 26 1 27 1
108.8 29.5 29.6 53.8 32 32
48.9 10.7 11.6 17.5 15.9 14.9
6 6 6 6 6 6
28 1 29 2 30 2 31 4 32 1
36.1 59.2 60.2 108.1 40.5
16 20.1 20.7 44.7 23.6
6 6 6 6 6
33 1 34 1 35 1 36 2 37 1 38 2 39 2
33.3 33.8 40.8 56.5 30.8 50.5 55.7
14.2 14.8 23.1 16.1 14.5 18.1 25.1
6 6 6 6 6 6 6
Food intake g /day/m ouse
3.00 3.54 3.01 3.08 2.66
3.06 0.32
2.87 3.83 3.22 2.60 2.65 3.04 0.51
2.90 3.10 3.23 3.12 2.93 2.73 3.12
3.02 0.17 3.46 2.63 3.50 2.94 3.13 0.42 2.50 3.13 3.00 3.03 2.68 2.85 2.86 0.24
3.35 3.26 3.29 2.64 2.82
3.07 0.32 3.18 3.17 2.95 3.37 2.72 2.70 2.55 2.95 0.30
Food on cage w eek 1 (g) 51.4 52.3 51.1 29.7 83.6
Food off cage week 2 (g) 12 9.9 10.9 10.5 31.4
80.7 28.5 81.3 52.9 31.1
24.4 4.3 20.1 18 12.1
26.4 31.4 60 27.6 76.3 27.9 28.3
6.1 11.6 16.2 7.8 23.8 8.2 8.5
50.9 98.6 48.6 56.8
11.4 33.7 8.1 17.8
100.7 24.9 27.2 49.2 25.6 29.5
40.6 5.6 11.2 14.4 9 13
29 53.8 51.2 101.5 31.1
8.6 11.8 9.3 33 11.1
30.5 27.6 28.2 55.6 29.2 54.2 51.2
12.8 11.9 10.8 21 13.5 25.7 21.4
days
6 6 6 6 6
6 6 6 6 6
6 6 6 6 6 6 6
6 6 6 6
6 6 6 6 6 6
6 6 6 6 6
6 6 6 6 6 6 6
Food intake g/day/m ouse
3 .2 8 3 .5 3 3 .3 5 3 .2 0 2 .9 0
3 .2 5 0 .2 3
3 .1 3 4 .0 3 3 .4 0 2.91 3 .1 7 3 .3 3 0 .4 3
3 .3 8 3 .3 0 3 .6 5 3 .3 0 2 .9 2 3 .2 8 3 .3 0
3 .3 0 0.21 3 .2 9 2 .7 0 3 .3 8 3 .2 5 3 .1 6 0.31 2 .5 0 3 .2 2 2 .6 7 2 .9 0 2 .7 7 2 .7 5 2 .8 0 0 .2 4
3 .4 0 3 .5 0 3 .4 9 2 .8 5 3 .3 3
3 .3 2 0 .2 7 2 .9 5 2 .6 2 2 .9 0 2 .8 8 2 .6 2 2 .3 8 2 .4 8 2 .6 9 0 .2 2
Food on cage week 2 (g) 57.2 55 55.7 27.6 75.7
Food off cage week 2.3
days
77.6 27 83.1 55.5 31.8
29.9 29.4 56.3 30.5 81.6 35.1 30.1
57.4 106.4 53.4 52.3
108.7 31.7 31.3 57.8 30.3 34.1
83.2 23.7 24.3 41.4
23 27.4
2.8 2.8 2.8 2.8 2.8 2.8
35.1 57.4 50.2 104.6 36
37.6 32.4 31.1 54.6 33.9 55.7 56.4
30.8 24.6 24.8
41 28.1 44.7 44.5
2.8 2.8 2.8 2.8 2.8 2.8 2.8
Food intake g/day/m ouse
2 .2 8 2 .8 6 2 .5 0 2 .9 3 2.61 2 .3 9 2 .5 9 0 .2 6
2 .4 3 2 .7 9 2 .2 5 2 .4 3 2 .0 7 1.96 2 .1 3 2 .2 9 0 .2 8
Food on cage week 2.3
Food off cage week 3 (g) 15.2 9.3 19.2 5.1 17.4
8.7 9.6 16.5 17.3 10.2
17.5 7.9 8.4 10.3 25.2 15.3 10.1
15.7 24.9
10 11.6
108.6 27.2 31.8 57.1 29.5 31.0
62.5 14.9 20.1 31.1 15.5 17.8
14.3 10.3 7.1 25.2 17.8
32.9 33.6 27.6 52.5 28.7 54.9 60.0
23 24.2 16.6 26.7 18.5 34.5 26.7
days
7 7 7 7 7
7 7 7 7 7
7 7 7 7 7 7 7
7 7 7 7
4.2 4.2 4.2 4.2 4.2 4.2
7 7 7 7 7
4.2 4.2 4.2 4.2 4.2 4.2 4.2
Food intake g/day/m ouse
3 .0 0 3 .2 6 2.61 3.21 2 .7 8
2 .9 7 0 .2 8
3 .2 8 2 .4 9 3 .1 7 2 .7 3 3 .0 9 2 .9 5 0 .3 3
1.77 3 .0 7 3 .4 2 2 .8 9 2 .6 9 2 .8 3 2 .8 6
2 .9 4 0 .2 8 2 .9 8 2.91 3 .1 0 2.91 2 .9 7 0 .0 9 2 .7 4 2 .9 3 2 .7 9 3 .1 0 3.33 3 .1 4 3 .0 0 0 .2 3
2 .9 7 3 .3 6 3 .0 8 2 .8 4 2 .6 0
2 .9 7 0 .2 8 2 .3 6 2 .2 4 2 .6 2 3 .0 7 2 .4 3 2 .4 3 3 .9 6 2 .7 3 0.61
Food on cage w e e k 3 (g) 64.6 59.6 59.6 30.6 80.4
Food o ff cage week 4 (g) 28.8 21 21.2 10.7 30.8
days
6 6 6 6 6
77.4 30.1 79 54.3 33.5
19.5 8.4 18.5 19.6 14
6 6 6 6 6
31.3 32.8 67.6 27.4 78.4 32.9 30.2
11 13.7 24.8 9.1 26.5 14 11.5
6 6 6 6 6 6 6
56.7 110.8 55.6 59.1
18.7 46.9 16.2 23.1
6 6 6 6
104.1 38.5 40.3 65.1 35.3 31.3
30.4 22.5 19.7 21.8 18.8 12.3
6 6 6 6 6 6
29 54.5 54.8 105.1 29.5
9.5 16.3 19.1 39 11.1
6 6 6 6 6
50.1 41.5 31.5 60.4 27.3 52.7 58.5
32.1 23.3 13.4 28.1 8.2 18.4 28.1
6 6 6 6 6 6 6
Food intake g/day/m ouse
2 .9 8 3 .2 2 3 .2 0 3 .3 2 2 .7 6
3 .0 9 0 .2 3
3 .2 2 3 .6 2 3 .3 6 2 .8 9 3 .2 5 3 .2 7 0 .2 6
3 .3 8 3 .1 8 3 .5 7 3 .0 5 2 .8 8 3 .1 5 3 .1 2
3 .1 5 0 .2 5 3 .1 7 2 .6 6 3 .2 8 3 .0 0 3 .0 3 0 .2 7 3 .0 7 2 .6 7 3 .4 3 3.61 2 .7 5 3 .1 7 3 .1 2 0 .3 7
3 .2 5 3 .1 8 2 .9 8 2 .7 5 3 .0 7
3 .0 5 0 .1 9 3 .0 0 3 .0 3 3 .0 2 2 .6 9 3 .1 8 2 .8 6 2 .5 3 2 .9 0 0 .2 2
Appendix to TNO-report of 3M-02
Final version
04-12-2006
G roup
cage#
1 1 1 1 1 AVERAGE SD 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 AVERAGE SD
1 2 3 4 5
6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21
22 23 24 25 26 27
28 29 30 31 32
33 34 35 36 37 38 39
number of m ice 2 2 2 1 3
3 1 3 2 1
1 1 2 1 3 1 1
2 4 2 2
4 1 1 2 1 1
1 2 2 4 1
1 1 1 2 1 2 2
Food on cage Food off cage
w eek 4 (g)
w eek 5 (g)
50.3
19.0
55.6
22.0
51.7
18.3
26.2
10.0
83.0
34.6
days
5.5 5.5 5.5 5.5 5.5
74.4 29.7 75.5 50.2 25.7
26.0 10.8 24.6 23.8 9.5
5.5 5.5 5.5 5.5 5.5
26.5 29.5 55.8 30.2 84.8 25.7 35.6
9.8 14.7 18.2 16.1 42.1 10.0 20.9
5.5 5.5 5.5 5.5 5.5 5.5 5.5
53.0 107.9 58.2 58.0
19.4 52.0 26.7 29.5
5.5 5.5 5.5 5.5
105.0 30.5 30.2 53.0 28.5 27.6
60.2 16.8 15.7 22.2 15.4 14.3
4.5 4.5 4.5 4.5 4.5 4.5
30.2 50.5 54.1 102.0 30.0
16.3 18.2 22.6 45.9 17.1
5.5 5.5 5.5 5.5 5.5
33.2 31.4 41.8 45.3 26.2 65.5 55.0
17.4 16.7 29.1 16.2 13.8 35.9 33.8
4.5 4.5 4.5 4.5 4.5 4.5 4.5
F ood intake g/day/m ouse
2.85 3.05 3.04 2.95 2.93 2.96 0.08 2.93 3.44 3.08 2.40 2.95 2.96 0.37 3.04 2.69 3.42 2.56 2.59 2.85 2.67 2.83 0.31 3.05 2.54 2.86 2.59 2.76 0.24 2.49 3.04 3.22 3.42 2.91 2.96 3.01 0.32 2.53 2.94 2.86 2.55 2.34 2.64 0.25 3.51 3.27 2.82 3.23 2.76 3.29 2.36 3.03 0.40
Food on cage Food off cage
w eek 5 (g)
w eek 6 (g)
60.2
22.1
60.7
19.5
57.2
16.8
31.6
10.7
77.1
19.2
85.4 37.4 85.2 51.9 33.6
19.8 12.4 14.2 13.6 13.4
36 37.4 55.9 35.4 89.8 27.4 31.2
15.7 16.2 7.1 15.6 32.7
8 9.4
57.7 106.9 53.4 57.7
16.5 33.2 11.1 17.7
109.2 41.4 32.5 61.1 32.8 31.6
32.3 20.1 10.6 15.5 12.5 10.2
36.3 60.8 52.6 105.5 34.8
16.6 20.3 12.3 36.7
16
33.2 35.4 39.9 78.1 31.1 61.2 52.9
12.7 16.3 20 38.8 12.1 24.9
15
days
7 7 7 7 7
7 7 7 7 7
7 7 7 7 7 7 7
7 7 7 7
7 7 7 7 7 7
7 7 7 7 7
7 7 7 7 7 7 7
Food intake g/day/m ouse
2.72 2.94 2.89 2.99 2.76 2.86 0.12 3.12 3.57 3.38 2.74 2.89 3.14 0.34 2.90 3.03 3.49 2.83 2.72 2.77 3.11 2.98 0.26 2.94 2.63 3.02 2.86 2.86 0.17 2.75 3.04 3.13 3.26 2.90 3.06 3.02 0.18 2.81 2.89 2.88 2.46 2.69 2.75 0.18 2.93 2.73 2.84 2.81 2.71 2.59 2.71 2.76 0.11
Food on cage w eek 6 (g) 78.3 73.4 67 38.3 94.8
Food off cage w eek 7 (g) 61.7 55.5 48.9 29.1 71
days
3 3 3 3 3
97.1 42
85.6 60.9 46.3
69.2 32.6 54.1 46.3 37.3
3 3 3 3 3
39.9 40.9 56.5 46.1 114.5 34.9 37.9
30.3 31.4 39.2 37.8 91.4 26.2 29.5
3 3 3 3 3 3 3
61.5 130.6
70 67.9
42.6 98.9 50.3 50.3
3 3 3 3
138.4 52.3 37.3 74.9 39.9 35.4
107.2 44.3 27.6 53.8 31.1 26.8
3 3 3 3 3 3
44.3 66.8
60 142 46.7
34.9 48.7 42.9 112.9
39
3 3 3 3 3
35.7 42.4 46.1 90.6 39.7 76.5 65.9
27.4 33.8 36.9 73.3 30.7 60.4 50.8
3 3 3 3 3 3 3
F ood intake g/day/m ouse
2.77 2.98 3.02 3.07 2.64 2.90 0.18 3.10 3.13 3.50 2.43 3.00 3.03 0.39 3.20 3.17 2.88 2.77 2.57 2.90 2.80 2.90 0.22 3.15 2.64 3.28 2.93 3.00 0.28 2.60 2.67 3.23 3.52 2.93 2.87 2.97 0.35 3.13 3.02 2.85 2.43 2.57 2.80 0.30 2.77 2.87 3.07 2.88 3.00 2.68 2.52 2.83 0.19
Page A9 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group
cage#
mouse# Food on cage Food off cage days Food intake Food on cage
week 7 (g) week 7.5 (g)
g/day/mouse week 7.5(g)
11 1
438.1
428.4
3
3.22
29.8
1 MC 101
2
metabolic cage
20.3
1 MC 105 3
1 MC 201
4
metabolic cage metabolic cage
18.6 23.5
13
5
485.4 | 476.5
3
2.96
26.1
1 MC 206 6
metabolic cage
19.2
14
7
496.1
3 2.86
1
5 8,9,10
425.4
397.3
3
3.12
29.2 54.7
AVERAGE
3.04
SD 0.16
2
6 11,12,13
435
407.6
3
3.04
55.9
2 7 14
429.7
421.0
3
2.91
21.9
2
8 15,16,17
475.5
442.1 3 3.71
44.3
2
9 18,19
450.2
431.3
3
3.15
33.6
2 10 20
425.7
9.5 3 2.94
32.1
AVERAGE
3.15
SD 0.33
3
MC 102
21
metabolic cage
35.6
3
MC 106
22
metabolic cage
29.4
3 13 23
455.8 | 445.8
3
3.35
33.1
3
MC 202
24
3 14 25
metabolic cage
430.2
418.8
3
3.79
26.8 36.9
3 15 26,28 465.3
449.8
3
2.58
45.1
3
MC 206
27
metabolic cage
32.9
3 16 29 3 17 30
437.8 429.1
428.5 421.5
3 3
3.10 2.53
26.3 44.1
AVERAGE
3.07
SD 0.53
4
MC 103
31
metabolic cage
44.3
4
MC 107
32
metabolic cage
51.2
4
19 33,34,35,36
486.1 | 448.3
3
3.15
84.3
4
MC 203
37
metabolic cage
33.1
4 MC207 38
metabolic cage
32.6
4 21 39,40 436.2
419.7
3
2.75
44.2
AVERAGE
2.95
SD 0.28
5
22 41,42,43,44
505.9
470.3
3
2.97
109.2
5 23 45
443.9
434.1 3 3.26
41.4
5 24 46
438.7
428.7
3
3.35
32.5
5 25 47,48 443.3
423.9
3
3.24
61.1
5 26 49 5 27 50
428.6 436.1
419.7 427.5
3 3
2.98 2.86
32.8 31.6
AVERAGE SD
3.11 0.20
6 MC104 51
metabolic cage
38.2
6 29 52
432.6 | 424.2
3
2.81
27.6
6
MC 108
53
metabolic cage
33.1
6 MC204 54
metabolic cage
28.4
6 MC208 55
metabolic cage
33.1
6
31 56,57,58,59
409.8
371.4
3
3.20
87.4
6 32 60
441.6
433.5
3
2.69
21.7
AVERAGE SD
2.90 0.27
7 33 61
439.2
429.8
3
3.12
33.1
7 34 62
431.4
422.0
3
3.15
32.5
7 35 63
442.7
434.7
3
2.67
39.4
7 36 64,65 445.6
427.0
3
3.10
66.1
7 37 66
426.1
7 38 67,68 465.5
417.7 446.0
3 3
2.79 3.25
30.2 65.8
7 39 69,70 445.2
423.9
3
3.55
52.1
AVERAGE SD
3.09 0.29
NB: week 7-7.5: all mice are put on powdered food because of metabolic cages of part of mice
Food off cage week 8 (g) 15.7 12.8 8.7 15.0 13.9 8.3 18.2 25.8
12.5 11.9 11.7 8.4 17.3
26.2 21.4 20.3 17.7 24.7 20.5 25.1 15.5 34.7
35.6 43.3 36.0 23.1 23.2 27.0
63.4 31.1 18.9 38.2 20.9 22.2
27.5 15.6 25.6 20.7 24.7 46.3 11.4
22.4 22.5 27.5 39.3 18.8 40.7 32.5
days
4 3 3 3 4 3 4 4
4 4 4 4 4
3 3 4 3 4 4 3 4 4
3 3 4 3 3 4
4 4 4 4 4 4
3 4 3 3 3 4 4
4 4 4 4 4 4 4
Food intake g/day/mouse
3.52 2.51 3.29 2.84 3.05 3.63 2.75 2.41
3.00 0.45 3.62 2.51 2.72 3.15 3.70 3.14 0.53
3.12 2.67 3.20 3.02 3.06 3.08 2.61 2.71 2.36 2.87 0.29 2.89 2.62 3.02 3.34 3.14 2.15
2.86 0.42 2.86 2.57 3.41 2.86 2.98 2.36 2.84 0.36
3.58 3.00 2.49 2.57 2.81 2.57 2.58
2.80 0.39 2.68 2.51 2.98 3.35 2.84 3.14 2.45 2.85 0.33
Page A10 of A172
Appendix to TNO-report of 3M-02
Final version
G roup Mouse# cage#
AVERAGE SD 2 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3 3
AVERAGE SD
4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
1 1 2 2 3 3 4 5 5 5
6 6 6 7 8 8 8 9 9 10
11 12 13 13 14 15 15 15 16 17
18 18 19 19 19 19 20 20 21 21
22 22 22 22 23 24 25 25 26 27
28 29 29 30 30 31 31 31 31 32
33 34 35 36 36 37 38 38 39 39
Food on cage Food off cage
w e ek 8 (g)
w e e k 9 (g)
34.3 27.1
11.7 5.8
28.6
7.9
44.1
24.6
36.9 43.3
15.6 20.5
42.2
22.2
34.2
10.1
33.0 32.9
12.1 8.6
days
7 7 7 7 7 7 7 7 7 7
34.1 36.5 28.4 29.5 28.3 27.1 36.0 44.3 45.2 33.9
9.5 16.4 6.3 11.4 5.8 3.2 15.9 22.2 25.0 9.3
7 7 7 7 7 7 7 7 7 7
34.0 35.1 29.8 27.6 44.1 44.3 47.2 34.9 33.2 32.6
12.0 14.2 7.7 8.2 18.8 23.4 26.3 13.8 13.1 12.6
7 7 7 7 7 7 7 7 7 7
33.1 34.2 45.7 36.0 45.7 44.1 36.9 32.1 22.5 33.4
11.5 15.5 24.8 14.0 23.0 25.6 17.2 10.7 1.2 12.3
7 7 7 7 7 7 7 7 7 7
44.1 37.7 45.1 44.2 35.6 43.2 44.8 42.5 29.6 34.3
19.5 18.9 21.2 25.6 15.2 19.8 26.1 18.6 7.9 15.6
7 7 7 7 7 7 7 7 7 7
33.9 32.4 35.6 41.8 42.1 44.0 43.3 32.5 28.2 29.4
10.7 14.6 18.6 19.9 22.0 23.1 23.1 9.8 7.1 12.0
7 7 7 7 7 7 7 7 7 7
34.4 45.2 30.1 29.8 27.2 33.4 31.5 46.2 44.1 32.5
14.3 21.3 8.8 7.7 3.8 12.5 10.2 26.1 23.2 14.4
7 7 7 7 7 7 7 7 7 7
Food intake g/day/m ouse
3.23 3.05 2.96 2.79 3.05 3.26 2.86 3.45 2.98 3.47 3.11 0.23 3.52 2.87 3.16 2.58 3.22 3.42 2.87 3.16 2.88 3.52 3.12 0.32 3.15 2.98 3.16 2.77 3.62 2.99 2.99 3.01 2.87 2.86 3.04 0.24 3.08 2.67 2.98 3.15 3.24 2.65 2.81 3.06 3.04 3.02 2.97 0.20 3.52 2.68 3.42 2.66 2.91 3.35 2.67 3.42 3.10 2.67 3.04 0.36 3.32 2.55 2.43 3.13 2.87 2.98 2.88 3.24 3.01 2.49 2.89 0.31 2.87 3.41 3.05 3.16 3.34 2.99 3.04 2.87 2.99 2.58 3.03 0.24
04-12-2006
Page A11 of A172
o
(>
O
01 01
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0-1 1-2 2-3 (gr 5,7: 2.3-3) 3-4 4-5 5-6 6-7 7-7.5 7.5-8 8-9
week
0-1 1-2 2-3 (gr 5,7: 2.3-3) 3-4 4-5 5-6 6-7 7-7.5 7.5-8 8-9
week
0-1 1-2 2-2.3 2-3 (gr 5,7: 2.3-3) 3-4 4-5 5-6 6-7 7-7.5 7.5-8 8-9
Food intake, p va lu e s M a n n -W h itn e y U -test
group 1 vs 2
group 1 vs 3
group 1 vs 4
group 1 vs 5
group 1 vs 6
nn p nn p nn p nn E nn 5 5 0.690 5 7 0.876 5 4 0.905 5 6 0.429 5 5
p 1.000
5 5 1.000 5 7 0.530 5 4 0.905 5 6 0.017 5 5 0.690
5 5 1.000 5 7 0.755 5 4 0.905 5 6 0.931 5 5 1.000
5 5 0.222 5 7 0.876 5 4 0.730 5 6 0.931 5 5 0.548
5 5 0.841 5 7 0.202 5 4 0.286 5 6 0.662 5 5 0.056
5 5 0.222 5 7 0.432 5 4 0.222 5 6 0.126 5 5 0.222
5 5 0.310 5 7 1.000 5 4 0.310 5 6 1.000 5 5 0.690
4 5 0.905 4 5 1.000 4 2 0.800 4 6 0.352 4 3 0.400
8 5 0.622 8 9 0.606 8 6 0.755 8 6 0.662 8
0 .4 6 3
10 10 0.912 10 10 0.529 10 10 0.353 10 10 0.579 10
group 2 vs 4
group 2 vs 5
group 2 vs 6
group 2 vs 7
group 3 vs 4
nn p nn p nn p nn p nn
p
5 4 0.730 5 6 0.792 5 5 0.690 5 7 1.000 7 4 0.648
5 4 0.905 5 6 0.030 5 5 0.690 5 7 0.005 7 4 0.315
5 4 0.905 5 6 0.792 5 5 1.000 5 7 0.149 7 4 0.230
5 4 0.286 5 6 0.429 5 5 0.151 5 7 0.030 7 4 0.527
5 4 0.413 5 6 0.931 5 5 0.151 5 7 0.876 7 4 0.788
5 4 0.286 5 6 0.792 5 5 0.056 5 7 0.030 7 4 0.648
5 4 1.000 5 6 0.792 5 5 0.421 5 7 0.149 7 4 0.527
5 2 0.571 5 6 0.931 5 3 0.393 5 7 1.000 5 2 1.000
5 6 0.429 5 6 0.429 5 7 0.268 5 7 0.268 9 6 0.955
10 10 0.247 10 10 0.631 10 10 0 .165 10 10 0 .436 10 10 0.971
group 3 vs 7
group 4 vs 5
group 4 vs 6
group 4 vs 7
group 5 vs 6
n n p nn
pn
7 7 0.805 4 6 J 0.476 4
1 I I I i i l7 7 0.001 4 6 1 0 .114 4
7~
0.383 4 6 0.762 4
n 5
l5
5
pn
0.730 4
m0 .1 9 0 4
0.905 4
n ZJ
7
p 0.527 0.042
0 .2 3 0
nn
p
6 5 0.329
1 16 5 0.017
6 5 0.931
7 7 0.026 4 6 0.610 4 5 1.000 4 7 0.527 6 5 0.931
7 7 0.318 4 6 0.352 4 5 0.413 4 7 0.315 6 5 0.082
7 7 0.073 4 6 0.171 4 5 0.413 4 7 0.230 6 5 0.030
7 7 0.535 4 6 0.762 4 5 0.286 4 7 0.315 6 5 0.429
5 7 0.755 2 6 0.429 2 3 1.000 2 7 0.667 6 3 0.167
9 7 0.918 6 6 0.699 6 7 0.445 6 7 0.836 6 7 0.836
10 10 0.684 10 10 0.631 10 10 0.529 10 10 0.739 10 10 0.315
group 1 vs 7
nn
p
5 7 0.755
5 7 0.005
5 7 0.202
5 7 0.202
5 7 0.755
5 7 0.149
5 7 0.639
4 7 0.788
8 7 0.613
10 10 0.631
group 3 vs 5
nn
p
7 6 0.295
7 6 0.002
7 6 0.445
7 6 0.836
7 6 0.366
7 6 0.445
7 6 0.731
5 6 0.931
9 6 0.607
10 10 0.796
group 5 vs 7
nn
p
6 7 0.534
1 6 7 0.445 6 7 0.073
6 7 0.101
6 7 0.295
6 7 0.836
6 7 0.014
6 7 0.628
6 7 0.836
6 7 0.945
10 10 0.853
group 2 vs 3
nn
5T 7 5T T 5T T 5T T 5T T 5T T 5T T
p 0 .6 3 9 0 .7 5 5 0 .6 3 9 0.432 0.432 0.432 0 .5 3 0
5 5 1.000
5 9 0.298
0 .4 3 6
group 3 vs 6
nn
p
7 5 0.530
7 5 0.432
7 5 0.639
7 5 0.432
7 5 0.268
7 5 0.149
7 5 0.530
5 3 1.000
9 7 0.299
10 10 0.481
group 6 vs 7
nn
p
5 7 _ 0.530 5 7 0.018
5T 7 5T T 5T T 5T T 5T T 3T T
77
0.202 0 .2 6 8 0 .1 4 9 1.000 1.000 0.667 0 .8 0 5 0 .3 1 5
p < 0 .0 5
Page A12 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Appendix C. Plasma measurements
C1. Plasma cholesterol measurements
group
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD e x c lu d e d e x c lu d e d e x c lu d e d e x c lu d e d ex c luded
Mouse# cage#
11 21 32 42 53 63 74 85 95 10 5
11 6 12 6 13 6 14 7 15 8 16 8 17 8 18 9 19 9 20 10
21 11 22 12 23 13 24 13 25 14 26 15 27 15 28 15 29 16 30 17
31 18 32 18 33 19 34 19 35 19 36 19 37 20 38 20 39 21 40 21
41 22 42 22 43 22 44 22 45 23 46 24 47 25 48 25 49 26 50 27
51 28 52 29 53 29 54 30 55 30 56 31 57 31 58 31 59 31 60 32
61 33 62 34 63 35 64 36 65 36 66 37 67 38 68 38 69 39 70 39
655 656 741 742 748
85 86 96 96 97
eartag LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
12 00 21 02 20
M easured cholesterol (m m ol/l)
d ilu tio n
week 0 week 1 week 4 week 8 week 0 week 1 week 4 week 8 week 0
3.219 2.814 2.172 2.211
2
2
3
3 6.438
3.446 3.088 2.599 2.394
2
2
3
3 6.892
3.633 3.176 2.120 1.725
2
2
3
3 7.266
3.260 2.556 1.911 1.877
2
2
3
3 6.520
1.847 2.612 2.042 1.733
2
2
3
3 3.694
3.819 3.458 2.033 2.500
2
2
3
3 7.638
4.055 3.958 1.815 2.166
2
2
3
3 8.110
3.949 3.668 2.347 2.295
2
2
3
3 7.898
4.590 3.257 1.972 1.816
2
2
3
3 9.180
3.706 3.015 1.911 1.854
2
2
3
3 7.412
7.10 1.45
3.998 3.901 2.625 2.181
2
2
3
3 7.996
1.791 2.145 1.110 0.867
2
2
3
3 3.582
4.428 3.281 2.094 2.287
2
2
3
3 8.856
4.274 2.878 1.859 1.353
2
2
3
3 8.548
4.453 3.080 2.443 2.272
2
2
3
3 8.906
1.774 2.306 1.057 0.837
2
2
3
3 3.548
1.750 2.322 1.563 1.209
2
2
3
3 3.500
4.079 3.450 2.233 2.151
2
2
3
3 8.158
4.055 2.959 2.233 2.439
2
2
3
3 8.110
3.633 3.233 2.085 2.029
2
2
3
3 7.266
6.85 2.33
4.201 3.217 1.850 2.439
2
2
3
3 8.402
3.446 3.007 1.876 2.021
2
2
3
3 6.892
4 .0 4 7 3 .2 5 7 2 .0 7 7 1.771
2
2
3
3 8.094
4.071 2.999 1.920 2.318
2
2
3
3 8.142
3.649 2.685 1.406 1.763
2
2
3
3 7.298
3.893 2.741 1.885 1.870
2
2
3
3 7.786
3.130 2.798 1.737 1.703
2
2
3
3 6.260
3.519 3.201 1.850 2.219
2
2
3
3 7.038
4.290 3.466 2.521 2.431
2
2
3
3 8.580
2.083 1.968 1.153 1.315
2
2
3
3 4.166
7.27 1.31
3.974 1.565 1.327 1.125
2
2
3
3 7.948
3.625 1.622 1.327 1.353
2
2
3
3 7.250
4.104 2.057 1.476 1.422
2
2
3
3 8.208
3.122 1.912 1.284 1.186
2
2
3
3 6.244
3.698 2.322 1.380 1.490
2
2
3
3 7.396
1.701 1.904 1.301 1.209
2
2
3
3 3.402
3.576 1.895 1.467 1.452
2
2
3
3 7.152
3.511 1.879 1.354 1.156
2
2
3
3 7.022
3.917 2.467 1.754 1.672
2
2
3
3 7.834
2.010 1.557 1.293 1.429
2
2
3
3 4.020
6.65 1.65
1.661 1.871 1.179 1.072
2
2
3
3 3.322
3.617 1.525 1.197 1.885
2
2
3
3 7.234
3.682 1.839 1.162 1.983
2
2
3
3 7.364
3.795 1.670 1.188 1.490
2
2
3
3 7.590
4.323 3.056 1.258 2.591
2
2
3
3 8.646
3.901 2.226 1.388 2.454
2
2
3
3 7.802
3.641 1.485 0.813 0.746
2
2
3
3 7.282
3.065 1.638 1.449 1.429
2
2
3
3 6.130
1.669 2.008 1.354 1.247
2
2
3
3 3.338
2.708 2.274 1.467 2.097
2
2
3
3 5.416
6.41 1.85
2.862 1.992 1.101 1.794
2
2
3
3 5.724
3.138 2.604 1.101 1.247
2
2
3
3 6.276
4.086 2.556 1.223 1.953
2
2
3
3 8.172
3 .3 1 6 2 .2 5 8 1.1 1 0 1.611
2
2
3
3 6.632
3.511 2.492 1.136 1.543
2
2
3
3 7.022
3.584 2.508 1.110 1.968
2
2
3
3 7.168
1.523 1.654 1.214 0.928
2
2
3
3 3.046
3.795 2.330 1.005 1.452
2
2
3
3 7.590
3.357 2.024 1.249 1.763
2
2
3
3 6.714
3.365 2.065 1.310 2.386
2
2
3
3 6.730
6.51 1.39
3.462 1.646 2.338 2.113
2
2
3
3 6.924
3.763 1.775 3.174 2.021
2
2
3
3 7.526
3.024 1.541 2.573 2.447
2
2
3
3 6.048
3.479 1.549 2.242 2.280
2
2
3
3 6.958
3.324 1.501 1.920 1.816
2
2
3
3 6.648
3.357 1.581 2.077 1.832
2
2
3
3 6.714
4.241 1.879 1.998 1.733
2
2
3
3 8.482
4.274 1.928 1.963 2.158
2
2
3
3 8.548
3.917 1.839 1.824 1.695
2
2
3
3 7.834
3.795 1.783 1.894 1.505
2
2
3
3 7.590
7.33 0.81
6.230
2 12.460
1.336 2 2.672
1.360 2 2.720 0.792 2 1.584
0 .7 7 6
2
1.552
C holesterol (m m ol/l)
week 1
week 4
5.628
6.516
6.176 6.352
7.797 6.360
5.112
5.733
5.224 6.916
6.126 6.099
7.916 7.336
5.445 7.041
6.514 6.030
5.916 5.733
6.32 0.90
6.28 0.70
7.802 4.290
7.875 3.330
6.562
6.282
5.756 6.160
5.577 7.329
4.612 4.644
3.171 4.689
6.900
6.699
5.918 6.466
6.699 6.255
5.91 1.12
5.79 1.60
6.434
5.550
6.014
5.628
6.514 5.998
6.231 5.760
5.370 5.482
4.218 5.655
5.596
5.211
6.402 6.932 3.936
5.550 7.563 3.459
5.87 0.84
5.48 1.10
3.130
3.981
3.244 4.114
3.981 4.428
3.824 4.644
3.852 4.140
3.808
3.903
3.790 3.758
4.401 4.062
4.934 3.114
5.262 3.879
3.84 0.61
4.19 0.43
3.742 3.050
3.537 3.591
3.678
3.486
3.340 6.112
3.564 3.774
4.452 2.970
4.164 2.439
3.276 4.016
4.347 4.062
4.548
4.401
3.92 0.94
3.74 0.57
3.984
3.303
5.208
3.303
5.112 4.516
3.669 3.330
4.984 5.016
3.408 3.330
3.308
3.642
4.660 4.048 4.130
3.015 3.747 3.930
4.50 0.62
3.47 0.27
3.292
7.014
3.550 3.082
9.522 7.719
3.098
6.726
3.002 3.162
5.760 6.231
3.758 3.856
5.994 5.889
3.678 3.566
5.472 5.682
3.40 0.31
6.60 1.24
week 8 6.633 7.182 5.175 5.631 5.199 7.500 6.498 6.885 5.448 5.562 6.17 0.87 6.543 2.601 6.861 4.059 6.816 2.511 3.627 6.453 7.317 6.087 5.29 1.88 7.317 6.063 5.313 6.954 5.289 5.610 5.109 6.657 7.293 3.945 5.96 1.10 3.375 4.059 4.266 3.558 4.470 3.627 4.356 3.468 5.016 4.287 4.05 0.53 3.216 5.655 5.949 4.470 7.773 7.362 2.238 4.287 3.741 6.291 5.10 1.81 5.382 3.741 5.859 4.833 4.629 5.904 2.784 4.356 5.289 7.158 4.99 1.23 6.339 6.063 7.341 6.840 5.448 5.496 5.199 6.474 5.085 4.515 5.88 0.88
1
Page A13 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0 1 4 8
week
0 1 4 8
week
0 1 4 8
group 1 vs 2
nn
p
10 10 0.631
10 10 0.436 10 10 0.912
10 10 0.436 group 2 vs 4
nn p 10 10 0.247
10 10 <0.001 10 10 0.029
10 10 0.190 group 3 vs 7
nn
p
10 10 0.739
10 10 <0.001
10 10 0.023
10 10 0.796
group 1 vs 3 nn p 10 10 0.631 10 10 0.393 10 10 0.035 10 10 0.796 group 2 vs 5 nn p 10 10 0.218 10 10 <0.001 10 10 0.015 10 10 0.796 group 4 vs 5 nn p 10 10 0.739 10 10 0.739 10 10 0.089 10 10 0.165
Plasma cholesterol, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn p nn E nn
p
nn
p
10 10 0.631 10 10 0.393 10 10 0.247 10 10 0.912
10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 <0.001
10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 0.853
10 10 <0.001 10 10 0.247 10 10 0.035 10 10 0.393
group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
n n p n n p nn 10 10 0.190 10 10 0.529
p
nn
p
0.353 10 10 0.315
0000
10 10 0.009 10 10 <0.001
<0.001 10 10 0.001
10 10 0.007 10 10 0.579
0.005 10 10 0.002
10 10 0.579 10 10 0.853
<0.001 10 10 0.393
group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn
p
nn
p
nn
10 10 0.393 10 10 0.654
p
nn
p
0.631 10 10 0.436
0000
10 10 0.015 10 10 0.052
0.052 10 10 0.247
10 10 <0.001 10 10 <0.001
0.089 10 10 <0.001
10 10 0.023 10 10 <0.001
0.853 10 10 0.315
group 2 vs 3 nn p 10 10 0.796 10 10 0.853 10 10 0.393 10 10 0.579 group 3 vs 6 nn p 10 10 0.123 10 10 0.001 10 10 <0.001 10 10 0.089 group 6 vs 7 nn p 10 10 0.190 10 10 <0.001 10 10 <0.001 10 10 0.089
p<0.05
1111 1111
Page A14 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
C2. Plasma triglyceride measurements
group
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD e x c lu d e d e x c lu d e d e x c lu d e d e x c lu d e d e x c lu d e d
Mouse# cage#
11 21 32 42 53 63 74 85 95 10 5
11 6 12 6 13 6 14 7 15 8 16 8 17 8 18 9 19 9 20 10
21 11 22 12 23 13 24 13 25 14 26 15 27 15 28 15 29 16 30 17
31 18 32 18 33 19 34 19 35 19 36 19 37 20 38 20 3 9 21 4 0 21
41 22 42 22 43 22 44 22 45 23 46 24 47 25 48 25 49 26 50 27
51 28 52 29 53 29 54 30 55 30 5 6 31 5 7 31 5 8 31 5 9 31 60 32
61 33 62 34 63 35 64 36 65 36 66 37 67 38 68 38 69 39 70 39
655 85 656 86 741 96 742 96 748 97
e a rta g LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
12 00 21 02 20
M easured T G (m m ol/l)
d ilu tio n
week 0 week 1 week 4 week 8 week 0 week 1 week 4 week 8
0.445 0.387 0.257 0.298
2
2
3
3
0.467 0.270 0.263 0.266
2
2
3
3
0 .9 5 0 0.731 0 .2 9 0 0 .2 3 3
2
2
3
3
0.706 0.609 0.329 0.277
2
2
3
3
1.666 0.583 0.214 0.277
2
2
3
3
0.645 0.472 0.345 0.244
2
2
3
3
0.595 0.625 0.247 0.471
2
2
3
3
0.495 0.482 0.208 0.293
2
2
3
3
0.562 0.477 0.296 0.233
2
2
3
3
0.756 0.456 0.186 0.212
2
2
3
3
0.584 0.434 0.274 0.255
2
2
3
3
0.695 0.667 0.356 0.336
2
2
3
3
0.639 0.445 0.219 0.255
2
2
3
3
0.961 0.535 0.219 0.222
2
2
3
3
1.366 0.842 0.340 0.461
2
2
3
3
0.833 0.688 0.422 0.655
2
2
3
3
0.417 1.080 0.515 0.585
2
2
3
3
0.589 0.376 0.257 0.201
2
2
3
3
0.467 0.477 0.252 0.320
2
2
3
3
0.501 0.355 0 .2 1 4 0.195
2
2
3
3
0.573 0.276 0.121 0.190
2
2
3
3
0.606 0.307 0.148 0.201
2
2
3
3
0.978 0.762 0.307 0.233
2
2
3
3
0.661 0.932 0.372 0.168
2
2
3
3
0.795 0.456 0.143 0.190
2
2
3
3
0.656 0.482 0.148 0.244
2
2
3
3
0.478 0.376 0.143 0.152
2
2
3
3
0.528 0.382 0.137 0.195
2
2
3
3
0.412 0.403 0.208 0.336
2
2
3
3
0.900 0.673 0.312 0.369
2
2
3
3
0.856 0.244 0.137 0.082
2
2
3
3
0 .6 3 4 0.281 0 .1 4 3 0 .0 8 2
2
2
3
3
0.445 0.276 0.121 0.087
2
2
3
3
0.506 0.233 0.099 0.103
2
2
3
3
0.589 0.207 0.132 0.093
2
2
3
3
0.489 0.297 0.230 0.206
2
2
3
3
0.739 0.329 0.132 0.082
2
2
3
3
0.994 0.419 0.148 0.082
2
2
3
3
0.562 0.270 0.104 0.103
2
2
3
3
0.673 0.339 0.186 0.190
2
2
3
3
0.229 0.376 0.121 0.120
2
2
3
3
0.456 0.212 0.132 0.228
2
2
3
3
0.512 0.165 0.099 0.206
2
2
3
3
1.194 0.202 0.132 0.222
2
2
3
3
0.928 0.218 0.099 0.358
2
2
3
3
0 .6 3 4 0.181 0 .1 1 0 0 .3 2 0
2
2
3
3
0.722 0.260 0.247 0.309
2
2
3
3
0.678 0.207 0.208 0.250
2
2
3
3
0.589 0.323 0.870 0.103
2
2
3
3
0.551 0.186 0 .1 1 5 0.260
2
2
3
3
0.728 0.424 0.110 0.131
2
2
3
3
0.545 0.773 0.170 0.125
2
2
3
3
0.850 0.620 0.208 0.201
2
2
3
3
1.227 0.916 0.159 0.136
2
2
3
3
0.667 0.720 0.170 0.212
2
2
3
3
0.606 0.487 0.159 0.228
2
2
3
3
0.168 0.244 0.154 0.114
2
2
3
3
0.628 0.588 0.154 0.179
2
2
3
3
0.578 0.419 0.154 0.168
2
2
3
3
0.467 0.366 0.126 0.325
2
2
3
3
1.349 0.196 0.263 0.168
2
2
3
3
0.717 0.228 0.361 0.163
2
2
3
3
0.650 0.249 0.356 0.212
2
2
3
3
0.856 0.244 0.203 0.217
2
2
3
3
0.784 0.265 0.236 0.260
2
2
3
3
0.539 0.329 0.318 0.212
2
2
3
3
0.606 0.202 0.312 0.201
2
2
3
3
0.428 0.265 0.225 0.379
2
2
3
3
0.573 0.302 0.230 0.212
2
2
3
3
0.473 0.255 0.132 0.136
2
2
3
3
1 .9 5 4 0 .9 7 8 0 .9 1 7 0.251 0 .2 5 6
2 2 2 2 2
week 0 0.89 0 .9 3 4 1.9 1 .4 1 2 3 .3 3 2 1.29 1.19 0.99 1 .1 2 4 1 .5 1 2 1.46 0.73 1 .1 6 8 1.39 1 .2 7 8 1 .9 2 2 2 .7 3 2 1 .6 6 6 0 .8 3 4 1 .1 7 8 0 .9 3 4 1 .0 0 2 1.41 0.57 1 .1 4 6 1 .2 1 2 1 .9 5 6 1 .3 2 2 1.59 1 .3 1 2 0 .9 5 6 1 .0 5 6 0 .8 2 4 1.8 1.32 0.36 1 .7 1 2 1 .2 6 8 0.89 1 .0 1 2 1 .1 7 8 0 .9 7 8 1 .4 7 8 1 .9 8 8 1 .1 2 4 1 .3 4 6 1.30 0.35 0 .4 5 8 0 .9 1 2 1 .0 2 4 2 .3 8 8 1 .8 5 6 1 .2 6 8 1 .4 4 4 1 .3 5 6 1 .1 7 8 1 .1 0 2 1.30 0.53 1 .4 5 6 1.09 1.7 2 .4 5 4 1 .3 3 4 1 .2 1 2 0 .3 3 6 1 .2 5 6 1 .1 5 6 0 .9 3 4 1.29 0.54 2 .6 9 8 1 .4 3 4 1.3 1 .7 1 2 1 .5 6 8 1 .0 7 8 1 .2 1 2 0 .8 5 6 1 .1 4 6 0 .9 4 6 1.40 0.53 3 .9 0 8 1 .9 5 6 1 .8 3 4 0 .5 0 2 0 .5 1 2
T G (m m ol/l)
week 1
week 4
0 .7 7 4 0 .5 4
0.771 0.789
1 .4 6 2
0.87
1 .2 1 8 1 .1 6 6
0.987 0.642
0 .9 4 4 1.25
1.035 0.741
0 .9 6 4 0 .9 5 4 0 .9 1 2
0.624 0.888 0.558
1.02 0 .2 6
0.79 0.16
0 .8 6 8
0.822
1 .3 3 4 0 .8 9
1.068 0.657
1.07 1 .6 8 4
0.657 1.02
1 .3 7 6 2 .1 6
1.266 1.545
0 .7 5 2 0 .9 5 4 0.71
0.771 0.756 0.642
1.18 0 .4 6
0.92 0.30
0 .5 5 2
0.363
0 .6 1 4 1 .5 2 4
0.444 0.921
1 .8 6 4 0 .9 1 2
1.116 0.429
0 .9 6 4 0 .7 5 2
0.444 0.429
0 .7 6 4
0.411
0 .8 0 6 1 .3 4 6
0.624 0.936
1.01 0 .4 3
0.61 0.27
0 .4 8 8
0.411
0 .5 6 2 0 .5 5 2
0.429 0.363
0 .4 6 6 0 .4 1 4
0.297 0.396
0 .5 9 4
0.69
0 .6 5 8 0 .8 3 8
0.396 0.444
0 .5 4 0 .6 7 8
0.312 0.558
0 .5 8 0 .1 2
0.43 0.12
0 .7 5 2 0 .4 2 4
0.363 0.396
0 .3 3 0 .4 0 4
0.297 0.396
0 .4 3 6 0 .3 6 2
0.297 0.33
0 .5 2 0 .4 1 4
0.741 0.624
0 .6 4 6 0 .3 7 2
2.61 0.345
0 .4 7 0 .1 4
0.42 0.16
0 .8 4 8 1 .5 4 6
0.33 0.51
1.24 1 .8 3 2
0.624 0.477
1.44 0 .9 7 4
0.51 0.477
0 .4 8 8
0.462
1 .1 7 6 0 .8 3 8 0 .7 3 2
0.462 0.462 0.378
1.11 0.41
0.47 0.08
0 .3 9 2 0 .4 5 6
0.789 1.083
0 .4 9 8 0 .4 8 8
1.068 0.609
0 .5 3
0.708
0 .6 5 8 0 .4 0 4
0.954 0.936
0 .5 3 0 .6 0 4 0.51
0.675 0.69 0.396
0.51 0 .0 8
0.79 0.22
week 8 0 .8 9 4 0 .7 9 8 0 .6 9 9 0.831 0.831 0 .7 3 2 1 .4 1 3 0 .8 7 9 0 .6 9 9 0 .6 3 6 0.84 0.22 0 .7 6 5 1 .0 0 8 0 .7 6 5 0 .6 6 6 1 .3 8 3 1 .9 6 5 1 .7 5 5 0 .6 0 3 0.96 0 .5 8 5 1.05 0.49 0.57 0 .6 0 3 0 .6 9 9 0 .5 0 4 0.57 0 .7 3 2 0 .4 5 6 0 .5 8 5 1 .0 0 8 1 .1 0 7 0.68 0.21 0 .2 4 6 0 .2 4 6 0.261 0 .3 0 9 0 .2 7 9 0 .6 1 8 0 .2 4 6 0 .2 4 6 0 .3 0 9 0.57 0.33 0.14 0.36 0 .6 8 4 0 .6 1 8 0 .6 6 6 1 .0 7 4 0.96 0 .9 2 7 0.75 0 .3 0 9 0.78 0.76 0.21 0 .3 9 3 0 .3 7 5 0 .6 0 3 0 .4 0 8 0 .6 3 6 0 .6 8 4 0 .3 4 2 0 .5 3 7 0 .5 0 4 0 .9 7 5 0.55 0.19 0 .5 0 4 0 .4 8 9 0 .6 3 6 0.651 0.78 0 .6 3 6 0 .6 0 3 1 .1 3 7 0 .6 3 6 0 .4 0 8 0.65 0.20
1
Page A15 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0 1 4 8
week
0 1 4 8
wee k
0 1 4 8
group 1 vs 2
nn
p
10 10 0.971
10 10 0.739
10 10 0.393
10 10 0.684
group 2 vs 4
nn p 10 10 0.912
10 10 <0.001
10 10 <0.001
10 10 <0.001
group 3 vs 7
nn
p
10 10 1.000
10 10 <0.001
10 10 0.123
10 10 1.000
group 1 vs 3 nn p 10 10 0.971 10 10 0.631 10 10 0.089 10 10 0.043 group 2 vs 5 nn p 10 10 0.796 10 10 <0.001 10 9 <0.001 10 10 0.218 group 4 vs 5 nn p 10 10 1.000 10 10 0.029 10 9 0.400 10 10 <0.001
Plasma triglycerides, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn
p nn E nn
p
nn
p
10 10 0.912 10 10 0.796 10 10 0.912 10 10 0.912
10 10 <0.001 10 10 <0.001 10 10 0.739 10 10 <0.001
10 10 <0.001 10 9 <0.001 10 10 <0.001 10 10 0.912
10 10 <0.001 10 10 0.353 10 10 0.002 10 10 0.007
group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
nn p nn p
nn
p
nn
p
10 10 0.853 10 10 0.971 10 10 0.971 10 10 0.912
10 10 0.853 10 10 <0.001 10 10 0.002 10 10 <0.001
10 10 <0.001 10 10 0.529 10 10 0.063 10 9 0.017
10 10 0.003 10 10 0.029 10 10 0.001 10 10 0.529
group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn p nn p
nn
p
nn
p
10 10 0.971 10 10 0.853 10 10 0.971 10 10 0.796
10 10 0.001 10 10 0.143 10 10 <0.001 10 10 0.190
10 10 0.143 10 10 0.001 9 10 0.133 9 10 0.001
10 10 0.004 10 10 <0.001 10 10 0.123 10 10 0.315
group 2 vs 3 nn p 10 10 0.971 10 10 0.393 10 10 0.023 10 10 0.029 group 3 vs 6 nn p 10 10 0.971 10 10 0.529 10 10 0.971 10 10 0.123 group 6 vs 7 nn p 10 10 0.796 10 10 0.190 10 10 0.001 10 10 0.315
p<0.05
Page A16 of A172
Appendix to TNO-report of 3M-02
Final version
C3. Pooled lipoprotein profile measurements
04-12-2006
Week 0
C h olesterol
fra ctio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured c h o le s te ro l (m M ) po o le d s a m p le 1
0 .0 0 8 0 .0 0 2 0.021 0 .0 4 3 0 .2 9 2 0 .2 9 5 0 .1 6 6 0 .1 0 9 0 .0 8 7 0 .1 0 9 0 .1 1 5 0 .1 4 7 0 .2 2 2 0 .2 6 6 0 .2 5 7 0 .2 1 6 0 .2 3 2 0 .2 9 5 0 .3 0 7 0 .2 2 9 0 .1 4 7 0 .0 9 3 0.071 0 .0 4 0
dilu tio n c h o le s te ro l (m M )
2 0.016 2 0.004 2 0.042 2 0.086 2 0.584 2 0.590 2 0.332 2 0.218 2 0.174 2 0.218 2 0.230 2 0.294 2 0.444 2 0.532 2 0.514 2 0.432 2 0.464 2 0.590 2 0.614 2 0.458 2 0.294 2 0.186 2 0.142 2 0.080
m easured c h o le ste ro l (m M ) po o le d s a m p le 2
0 .0 1 2 0 .0 0 8 0 .0 1 2 0 .0 6 8 0 .2 4 8 0 .2 3 5 0.131 0 .0 9 3 0 .0 7 5 0 .0 7 8 0 .1 0 0 0 .1 3 4 0 .1 8 8 0 .2 2 2 0 .2 2 9 0 .1 9 4 0 .2 1 0 0 .2 8 8 0 .2 8 8 0 .2 0 3 0 .1 2 2 0 .0 9 0 0 .0 4 9 0 .0 4 0
dilution c h o le s te ro l (m M )
2 0.024 2 0.016 2 0.024 2 0.136 2 0.496 2 0.470 2 0.262 2 0.186 2 0.150 2 0.156 2 0.200 2 0.268 2 0.376 2 0.444 2 0.458 2 0.388 2 0.420 2 0.576 2 0.576 2 0.406 2 0.244 2 0.180 2 0.098 2 0.080
m easured c h o le ste ro l (m M ) p o o le d s a m p le 3
0 .0 1 2 0 .0 1 5 0 .0 0 5 0 .0 6 2 0 .2 7 0 0 .2 8 5 0 .1 6 3 0 .1 4 4 0 .1 0 3 0 .1 0 3 0 .1 3 4 0.181 0 .2 8 2 0 .3 1 4 0 .2 7 9 0.241 0 .2 3 8 0 .3 3 9 0 .3 7 0 0 .2 7 0 0 .1 6 6 0 .0 9 3 0 .0 5 9 0 .0 4 0
dilu tio n c h o le s te ro l (m M )
2 0.024 2 0.030 2 0.010 2 0.124 2 0.540 2 0.570 2 0.326 2 0.288 2 0.206 2 0.206 2 0.268 2 0.362 2 0.564 2 0.628 2 0.558 2 0.482 2 0.476 2 0.678 2 0.740 2 0.540 2 0.332 2 0.186 2 0.118 2 0.080
A v e ra g e p lasm a cho le ste ro l (m m ol/l) 0.021 0 .0 1 7 0 .0 2 5 0 .1 1 5 0 .5 4 0 0 .5 4 3 0 .3 0 7 0.231 0 .1 7 7 0 .1 9 3 0 .2 3 3 0 .3 0 8 0.461 0 .5 3 5 0 .5 1 0 0 .4 3 4 0 .4 5 3 0 .6 1 5 0 .6 4 3 0 .4 6 8 0 .2 9 0 0 .1 8 4 0 .1 1 9 0 .0 8 0
sd
0 .0 0 5 0 .0 1 3 0 .0 1 6 0 .0 2 6 0 .0 4 4 0 .0 6 4 0 .0 3 9 0 .0 5 2 0 .0 2 8 0 .0 3 3 0 .0 3 4 0 .0 4 9 0 .0 9 5 0 .0 9 2 0 .0 5 0 0 .0 4 7 0 .0 2 9 0 .0 5 5 0 .0 8 6 0 .0 6 8 0 .0 4 4 0 .0 0 3 0 .0 2 2 0 .0 0 0
PL
fra ctio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured P L (m g/dl) po o le d s a m p le 1
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 3 .7 7 6 4 .4 2 9 2 .3 0 5 1 .7 0 6 1 .5 9 7 1 .6 5 2 1 .9 7 8 3 .5 0 3 5 .0 8 3 8 .6 2 3 7 .7 5 2 7.261 9 .0 5 9 1 2 .7 6 2 1 5 .1 0 4 1 2 .4 3 6 1 0 .0 9 4 5 .9 5 4 3 .9 3 9 1 .7 6 0
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
PL (m M )
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 5 8 0 .0 6 8 0 .0 3 6 0 .0 2 6 0 .0 2 5 0 .0 2 6 0.031 0 .0 5 4 0 .0 7 9 0 .1 3 3 0 .1 2 0 0 .1 1 2 0 .1 4 0 0 .1 9 7 0 .2 3 4 0 .1 9 2 0 .1 5 6 0 .0 9 2 0.061 0 .0 2 7
m easured P L (m g/dl) po o le d sa m p le 2
0 .0 0 0 0 .0 0 0 0 .0 0 3 0 .9 8 4 3 .5 1 3 4 .2 3 5 2 .5 8 4 2 .0 1 6 1 .7 0 6 1.861 2 .0 1 6 3 .4 0 9 5 .7 3 2 7 .0 2 2 8 .4 1 6 7 .4 3 5 8 .6 2 2 1 5 .2 2 9 1 5 .1 7 7 13.061 9 .9 1 3 7 .7 0 4 3 .8 2 2 2.171
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
PL (m M )
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 1 5 0 .0 5 4 0 .0 6 5 0 .0 4 0 0.031 0 .0 2 6 0 .0 2 9 0.031 0 .0 5 3 0 .0 8 9 0 .1 0 9 0 .1 3 0 0 .1 1 5 0 .1 3 3 0 .2 3 5 0 .2 3 5 0 .2 0 2 0 .1 5 3 0 .1 1 9 0 .0 5 9 0 .0 3 4
m easured PL (m g/dl) p o o le d s a m p le 3
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 4 .1 4 5 4 .1 9 9 2 .6 7 0 1 .8 5 0 1 .4 6 8 1 .7 9 6 2.561 3 .7 0 8 7 .3 1 3 9 .3 8 9 1 0 .0 4 4 8 .4 6 0 1 0 .0 9 9 1 7 .0 3 6 1 9 .1 6 7 1 5 .9 4 4 1 0 .9 1 8 7 .9 6 9 4 .4 1 8 1 .6 3 2
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
PL (m M )
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 6 4 0 .0 6 5 0.041 0 .0 2 9 0 .0 2 3 0 .0 2 8 0 .0 4 0 0 .0 5 7 0 .1 1 3 0 .1 4 5 0 .1 5 5 0.131 0 .1 5 6 0 .2 6 3 0 .2 9 6 0 .2 4 6 0 .1 6 9 0 .1 2 3 0 .0 6 8 0 .0 2 5
A v e ra g e p la s m a P L(m M )
sd
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 5 0 .0 5 9 0 .0 6 6 0 .0 3 9 0 .0 2 9 0 .0 2 5 0 .0 2 7 0 .0 3 4 0 .0 5 5 0 .0 9 3 0 .1 2 9 0 .1 3 5 0 .1 1 9 0 .1 4 3 0 .2 3 2 0 .2 5 5 0 .2 1 4 0 .1 5 9 0.111 0 .0 6 3 0 .0 2 9
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 9 0 .0 0 5 0 .0 0 2 0 .0 0 3 0 .0 0 2 0 .0 0 2 0 .0 0 2 0 .0 0 5 0 .0 0 2 0 .0 1 8 0 .0 1 9 0 .0 1 8 0 .0 1 0 0 .0 1 2 0 .0 3 3 0 .0 3 6 0 .0 2 9 0 .0 0 8 0 .0 1 7 0 .0 0 5 0 .0 0 4
Page A17 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week 4
C h olesterol
fractio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured cholestero l (mM )
pool group 1 0 .0 0 6 0 .0 0 6 0 .0 0 6 0 .0 2 8 0 .1 3 6 0 .1 3 9 0 .0 9 6 0 .0 7 4 0.071 0 .0 8 3 0 .1 2 6 0 .1 9 7 0 .2 7 2 0 .2 8 4 0.241 0 .2 0 4 0 .2 7 8 0 .4 4 2 0 .4 2 9 0 .2 6 5 0.151 0 .0 9 9 0 .0 6 5 0 .0 4 0
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholesterol (m M )
group 1 0 .0 1 2 0 .0 1 2 0 .0 1 2 0 .0 5 6 0 .2 7 2 0 .2 7 8 0 .1 9 2 0 .1 4 8 0 .1 4 2 0 .1 6 6 0 .2 5 2 0 .3 9 4 0 .5 4 4 0 .5 6 8 0 .4 8 2 0 .4 0 8 0 .5 5 6 0 .8 8 4 0 .8 5 8 0 .5 3 0 0 .3 0 2 0 .1 9 8 0 .1 3 0 0 .0 8 0
m easured cholestero l (m M )
pool group 2 0 .0 1 2 0 .0 0 9 0 .0 0 9 0 .0 6 2 0 .1 8 5 0 .1 5 7 0 .0 9 9 0 .0 7 4 0.071 0 .0 7 7 0 .1 2 0 0 .1 7 3 0 .2 2 5 0.231 0 .2 1 9 0 .2 0 7 0 .2 5 3 0 .3 5 8 0 .3 2 7 0 .2 0 4 0 .1 2 3 0 .0 8 0 0 .0 4 9 0 .0 3 4
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholestero l (m M )
group 2 0 .0 2 4 0 .0 1 8 0 .0 1 8 0 .1 2 4 0 .3 7 0 0 .3 1 4 0 .1 9 8 0 .1 4 8 0 .1 4 2 0 .1 5 4 0 .2 4 0 0 .3 4 6 0 .4 5 0 0 .4 6 2 0 .4 3 8 0 .4 1 4 0 .5 0 6 0 .7 1 6 0 .6 5 4 0 .4 0 8 0 .2 4 6 0 .1 6 0 0 .0 9 8 0 .0 6 8
m easured cholestero l (m M )
pool group 3 0 .0 0 9 0 .0 1 2 0 .0 0 9 0.031 0 .1 0 8 0 .1 4 2 0 .0 8 9 0 .0 7 7 0 .0 6 8 0 .0 8 3 0 .1 0 8 0 .1 5 7 0 .2 0 4 0 .2 0 7 0 .1 8 2 0 .1 7 9 0 .2 2 2 0 .3 3 7 0 .3 5 2 0 .2 3 8 0 .1 3 6 0 .0 8 6 0 .0 4 9 0.031
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholesterol (m M )
group 3 0 .0 1 8 0 .0 2 4 0 .0 1 8 0 .0 6 2 0 .2 1 6 0 .2 8 4 0 .1 7 8 0 .1 5 4 0 .1 3 6 0 .1 6 6 0 .2 1 6 0 .3 1 4 0 .4 0 8 0 .4 1 4 0 .3 6 4 0 .3 5 8 0 .4 4 4 0 .6 7 4 0 .7 0 4 0 .4 7 6 0 .2 7 2 0 .1 7 2 0 .0 9 8 0 .0 6 2
fra c tio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured cholesterol (m M )
pool group 4
0.020 0.014 0.027 0.030 0.064 0.058 0.048 0.030 0.027 0.030 0.054 0.107 0.215 0.324 0.324 0.271 0.231 0.203 0.160 0.107 0.064 0.045 0.036 0.024
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (m M )
group 4
0.040 0.028 0.054 0.060 0.128 0.116 0.096 0.060 0.054 0.060 0.108 0.214 0.430 0.648 0.648 0.542 0.462 0.406 0.320 0.214 0.128 0.090 0.072 0.048
m easured cholesterol (m M )
pool group 5
0.008 0.008 0.005 0.024 0.076 0.079 0.051 0.036 0.033 0.030 0.030 0.061 0.132 0.209 0.259 0.231 0.209 0.209 0.175 0.116 0.073 0.039 0.036 0.017
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (m M )
group 5
0.016 0.016 0.010 0.048 0.152 0.158 0.102 0.072 0.066 0.060 0.060 0.122 0.264 0.418 0.518 0.462 0.418 0.418 0.350 0.232 0.146 0.078 0.072 0.034
m easured cholesterol (m M )
pool group 6
0.011 0.008 0.011 0.024 0.054 0.073 0.039 0.036 0.027 0.030 0.036 0.064 0.135 0.215 0.249 0.218 0.197 0.184 0.160 0.110 0.064 0.039 0.027 0.014
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A ve ra g e cholesterol (m M )
group 6
0.022 0.016 0.022 0.048 0.108 0.146 0.078 0.072 0.054 0.060 0.072 0.128 0.270 0.430 0.498 0.436 0.394 0.368 0.320 0.220 0.128 0.078 0.054 0.028
m easured cholesterol (m M )
pool group 7
0.016 0.013 0.010 0.025 0.120 0.126 0.065 0.049 0.031 0.034 0.043 0.095 0.246 0.436 0.525 0.458 0.359 0.277 0.203 0.129 0.083 0.049 0.040 0.025
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A v e ra g e cholesterol (m M )
group 7
0.032 0.026 0.020 0.050 0.240 0.252 0.130 0.098 0.062 0.068 0.086 0.190 0.492 0.872 1.050 0.916 0.718 0.554 0.406 0.258 0.166 0.098 0.080 0.050
Page A18 of A172
Appendix to TNO-report of 3M-02
Final version
PL
fractio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured P L (m g/dl) pool group 1
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .1 1 3 0 .2 4 7 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .9 1 8 3.201 5 .7 5 2 7 .0 9 5 6 .2 8 9 6 .1 5 5 12.063 2 2 .8 0 4 2 3 .7 4 4 14.748 8 .3 0 3 4 .9 4 6 2.261 0 .2 4 7
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage PL (mM ) group 1
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 2 0 .0 0 4 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 1 4 0 .0 4 9 0 .0 8 9 0 .1 1 0 0 .0 9 7 0 .0 9 5 0 .1 8 6 0 .3 5 3 0 .3 6 7 0 .2 2 8 0 .1 2 8 0 .0 7 6 0 .0 3 5 0 .0 0 4
m easured P L(m g/dl) pool group 2
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 1.321 1.187 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .6 5 0 2 .6 6 4 4 .2 7 5 5 .8 8 6 5 .6 1 8 5 .8 8 6 10.317 17.702 17.433 10.586 5 .7 5 2 3 .8 7 2 1.187 0 .0 0 0
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage PL (m M ) group 2
0.000 0.000 0.000 0.000 0.020 0.018 0.000 0.000 0.000 0.000 0.010 0.041 0.066 0.091 0 .0 8 7 0.091 0.160 0.274 0.270 0.164 0.089 0.060 0.018 0.000
04-12-2006
m easured P L (m g/dl) pool group 3
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .5 1 6 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0.381 1.992 3 .6 0 4 4.141 4 .4 0 9 4 .6 7 8 8 .8 4 0 17.031 19.448 12.331 7 .3 6 3 4 .8 1 2 1.321 0 .0 0 0
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage PL (mM ) group 3
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 8 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 6 0.031 0 .0 5 6 0 .0 6 4 0 .0 6 8 0 .0 7 2 0 .1 3 7 0 .2 6 3 0.301 0.191 0 .1 1 4 0 .0 7 4 0 .0 2 0 0 .0 0 0
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured PL(m g/dl) pool group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.939 4.149 8.162 9.232 8.162 6.557 6.825 5.487 2.946 1.340 0.939 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.064 0.126 0.143 0.126 0.101 0.106 0.085 0.046 0.021 0.015 0.000 0.000
m easured PL(m g/dl) pool group 5
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 1.742 4 .5 5 1 6.825 6.825 6.022 7.226 6.022 3 .4 8 1 1.875 0.672 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 5
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.027 0.070 0.106 0.106 0.093 0.112 0.093 0.054 0.029 0.010 0.000 0.000
m easured PL(m g/dl) pool group 6
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 1.742 4.283 6.022 5 .6 2 1 5.219 5.487 5.086 2.812 1.207 0.538 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 6
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.027 0.066 0.093 0.087 0.081 0.085 0.079 0.043 0.019 0.008 0.000 0.000
m easured PL(m g/dl) pool group 7
0.000 0.000 0.000 0.000 0.000 0.187 0.000 0.000 0.000 0.000 0.000 0.858 5.690 11.998 16.024 15.085 12.400 10.253 7.435 4.348 2.603 1.529 0 .3 2 1 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 7
0.000 0.000 0.000 0.000 0.000 0.003 0.000 0.000 0.000 0.000 0.000 0.013 0.088 0.185 0.248 0.233 0.192 0.159 0.115 0.067 0.040 0.024 0.005 0.000
Page A19 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Week 6
C h oleste rol
fractio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured cholesterol (mM)
pool group 1
0 .0 1 0 0.001 0 .0 0 4 0 .0 2 5 0 .1 3 5 0 .1 3 5 0 .0 8 3 0 .0 6 5 0 .0 5 6 0 .0 7 4 0 .1 2 9 0 .2 2 3 0 .2 9 3 0 .2 9 3 0 .2 3 8 0 .1 8 3 0.241 0 .3 6 8 0 .3 4 7 0 .2 2 0 0 .1 3 2 0 .0 9 2 0 .0 6 2 0 .0 4 4
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholestero l (m M )
group 1
0 .0 2 0 0 .0 0 2 0 .0 0 8 0 .0 5 0 0 .2 7 0 0 .2 7 0 0 .1 6 6 0 .1 3 0 0 .1 1 2 0 .1 4 8 0 .2 5 8 0 .4 4 6 0 .5 8 6 0 .5 8 6 0 .4 7 6 0 .3 6 6 0 .4 8 2 0 .7 3 6 0 .6 9 4 0 .4 4 0 0 .2 6 4 0 .1 8 4 0 .1 2 4 0 .0 8 8
m easured cholestero l (m M )
pool group 2
0 .0 0 7 0 .0 0 7 0.004 0.050 0.174 0 .1 4 7 0.083 0.065 0.050 0.068 0.114 0.186 0.226 0.211 0.180 0 .1 6 2 0.220 0.274 0.241 0.153 0.086 0.059 0.035 0 .0 2 2
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholestero l (m M )
group 2
0 .0 1 4 0 .0 1 4 0 .0 0 8 0 .1 0 0 0 .3 4 8 0 .2 9 4 0 .1 6 6 0 .1 3 0 0 .1 0 0 0 .1 3 6 0 .2 2 8 0 .3 7 2 0 .4 5 2 0 .4 2 2 0 .3 6 0 0 .3 2 4 0 .4 4 0 0 .5 4 8 0 .4 8 2 0 .3 0 6 0 .1 7 2 0 .1 1 8 0 .0 7 0 0 .0 4 4
m easured cholesterol (m M )
pool group 3
0 .0 1 3 0 .0 1 3 0 .0 1 0 0 .0 6 2 0 .1 8 9 0 .1 6 8 0 .0 9 2 0 .0 7 7 0 .0 6 2 0 .0 8 3 0 .1 2 3 0 .1 8 0 0 .2 2 3 0.211 0 .1 6 2 0 .1 5 6 0 .2 3 5 0 .3 2 9 0 .2 9 6 0 .1 8 9 0 .1 1 4 0 .0 7 4 0 .0 4 7 0 .0 3 2
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholestero l (m M )
group 3
0 .0 2 6 0 .0 2 6 0 .0 2 0 0 .1 2 4 0 .3 7 8 0 .3 3 6 0 .1 8 4 0 .1 5 4 0 .1 2 4 0 .1 6 6 0 .2 4 6 0 .3 6 0 0 .4 4 6 0 .4 2 2 0 .3 2 4 0 .3 1 2 0 .4 7 0 0 .6 5 8 0 .5 9 2 0 .3 7 8 0 .2 2 8 0 .1 4 8 0 .0 9 4 0 .0 6 4
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured cholesterol (m M )
pool group 4
0.016 0.004 0.010 0.022 0.068 0.056 0.034 0.031 0.025 0.028 0.059 0.138 0.251 0.315 0.300 0.217 0.168 0.129 0.080 0.056 0.037 0.028 0.025 0.019
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (m M )
group 4
0.032 0.008 0.020 0.044 0.136 0.112 0.068 0.062 0.050 0.056 0.118 0.276 0.502 0.630 0.600 0.434 0.336 0.258 0.160 0.112 0.074 0.056 0.050 0.038
m easured
cholesterol (m M ) dilution
pool group 5
0.010
2
0.004
2
0.010
2
0.077
2
0.248
2
0.193
2
0.117
2
0.080
2
0 .0 7 1
2
0.077
2
0.129
2
0.220
2
0 .2 8 1
2
0 .2 5 1
2
0.202
2
0.156
2
0.205
2
0 .2 8 1
2
0.254
2
0.150
2
0.092
2
0.056
2
0.043
2
0.037
2
Average cholesterol (m M )
group 5
0.020 0.008 0.020 0.154 0.496 0.386 0.234 0.160 0.142 0.154 0.258 0.440 0.562 0.502 0.404 0.312 0.410 0.562 0.508 0.300 0.184 0.112 0.086 0.074
m easured cholesterol (m M )
pool group 6
0.016 0.025 0.016 0.037 0.071 0.071 0.037 0.034 0.031 0.037 0.053 0.120 0.214 0.266 0.266 0.211 0.156 0.126 0.080 0.049 0.037 0.025 0.016 0.013
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (m M )
group 6
0.032 0.050 0.032 0.074 0.142 0.142 0.074 0.068 0.062 0.074 0.106 0.240 0.428 0.532 0.532 0.422 0.312 0.252 0.160 0.098 0.074 0.050 0.032 0.026
m easured cholesterol (m M )
pool group 7
0.008 0.011 0.011 0.029 0.093 0.081 0.051 0.045 0.036 0.045 0.075 0.142 0.306 0.425 0.407 0.306 0.206 0.139 0.081 0.054 0.039 0.033 0.026 0.017
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (m M )
group 7
0.016 0.022 0.022 0.058 0.186 0.162 0.102 0.090 0.072 0.090 0.150 0.284 0.612 0.850 0.814 0.612 0.412 0.278 0.162 0.108 0.078 0.066 0.052 0.034
Page A20 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
PL
fraction 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL(mg/dl) pool group1
0.000 0.000 0.000 0.000 1.035 1.166 0.246 0.115 0.115 1.035 2.349 4.977 6.948 7.605 6.685 6.685 11.415 19.168 18.248 11.678 7.342 4.977 2.086 0.772
dilution 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL(mM) group1 0.000 0.000 0.000 0.000 0.016 0.018 0.004 0.002 0.002 0.016 0.036 0.077 0.107 0.118 0.103 0.103 0.176 0.296 0.282 0.181 0.114 0.077 0.032 0.012
measured PL(mg/dl) pool group2
0.000 0.000 0.000 0.000 1.823 1.297 0.903 0.640 0.246 0.509 1.823 4.057 5.502 6.159 5.897 6.028 9.313 13.649 12.729 8.525 4.714 2.874 1.035 0.115
dilution 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL(mM) group2 0.000 0.000 0.000 0.000 0.028 0.020 0.014 0.010 0.004 0.008 0.028 0.063 0.085 0.095 0.091 0.093 0.144 0.211 0.197 0.132 0.073 0.044 0.016 0.002
measured PL(mg/dl) pool group3
0.000 0.000 0.000 0.000 1.429 1.429 0.115 0.115 0.000 0.640 2.743 3.400 4.451 4.845 4.188 5.108 9.050 14.963 14.306 9.182 5.239 3.268 1.035 0.246
dilution 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL(mM) group3 0.000 0.000 0.000 0.000 0.022 0.022 0.002 0.002 0.000 0.010 0.042 0.053 0.069 0.075 0.065 0.079 0.140 0.231 0.221 0.142 0.081 0.051 0.016 0.004
fra c tio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured PL(m g/dl) pool group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.505 2.599 6.264 9.405 9.798 7.835 5.872 4.301 2.861 1.683 1.291 0.636 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.040 0.097 0.145 0.151 0.121 0.091 0.066 0.044 0.026 0.020 0.010 0.000 0.000
m easured PL(m g/dl) pool group 5
0.000 0.000 0.000 0.113 2.338 1.945 0.898 0.505 0.505 0.898 2.730 5.479 7.442 7.442 6.133 6.002 8.489 12.808 12.547 8.227 5.217 2.992 1.160 0.243
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage PL (m M ) group 5
0.000 0.000 0.000 0.002 0.036 0.030 0.014 0.008 0.008 0.014 0.042 0.085 0.115 0.115 0.095 0.093 0.131 0.198 0.194 0.127 0.081 0.046 0.018 0.004
m easured PL(m g/dl) pool group 6
0.000 0.000 0.000 0.000 0.000 0.505 0.000 0.000 0.000 0.000 0.374 2.338 5.348 7.966 8.882 7.049 5.610 4.039 2.599 1.552 0.898 0.505 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 6
0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.006 0.036 0.083 0.123 0.137 0.109 0.087 0.062 0.040 0.024 0.014 0.008 0.000 0.000
m easured PL(m g/dl) pool group 7
0.000 0.000 0.000 0.000 0.282 0.414 0.000 0.000 0.000 0.017 0.678 3.583 8.866 13.621 14.413 11.508 7.281 4.640 2.131 1.074 0.678 0.414 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 7
0.000 0.000 0.000 0.000 0.004 0.006 0.000 0.000 0.000 0.000 0.010 0.055 0.137 0.211 0.223 0.178 0.113 0.072 0.033 0.017 0.010 0.006 0.000 0.000
Page A21 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Week 8
C h olesterol
fractio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured cholestero l (mM )
pool group 1 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 3 6 0.191 0 .1 6 7 0 .1 0 3 0 .0 7 8 0 .0 7 8 0 .1 0 0 0 .1 6 4 0 .2 6 7 0 .3 2 8 0 .2 9 2 0 .2 4 0 0.191 0 .2 4 9 0 .3 4 6 0 .3 4 6 0 .2 0 0 0 .1 0 9 0 .0 6 6 0 .0 4 8 0 .0 2 7
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholestero l (m M )
group 1 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 7 2 0 .3 8 2 0 .3 3 4 0 .2 0 6 0 .1 5 6 0 .1 5 6 0 .2 0 0 0 .3 2 8 0 .5 3 4 0 .6 5 6 0 .5 8 4 0 .4 8 0 0 .3 8 2 0 .4 9 8 0 .6 9 2 0 .6 9 2 0 .4 0 0 0 .2 1 8 0 .1 3 2 0 .0 9 6 0 .0 5 4
m easured cholestero l (m M )
pool group 2 0 .0 0 0 0 .0 0 0 0 .0 0 2 0 .0 6 6 0 .2 2 8 0 .1 8 2 0 .1 0 9 0 .0 7 8 0 .0 6 9 0 .0 8 7 0 .1 3 9 0 .2 0 0 0 .2 4 9 0 .2 5 2 0 .2 3 7 0 .1 9 7 0 .2 3 7 0 .2 7 6 0 .2 4 0 0 .1 3 6 0 .0 7 8 0.051 0 .0 2 3 0 .0 2 0
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholestero l (m M )
group 2 0.000 0.000 0.004 0 .1 3 2 0.456 0.364 0.218 0.156 0.138 0.174 0.278 0.400 0.498 0.504 0.474 0.394 0.474 0 .5 5 2 0.480 0 .2 7 2 0.156 0 .1 0 2 0.046 0.040
m easured cholestero l (m M )
pool group 3 0 .0 0 0 0.011 0.011 0 .0 5 4 0 .2 0 6 0 .1 7 9 0 .1 1 2 0.091 0 .0 7 5 0 .1 1 2 0 .1 6 4 0 .2 4 6 0 .2 9 8 0 .2 7 3 0 .2 3 7 0 .1 8 8 0.231 0 .3 1 0 0 .2 8 8 0 .1 7 0 0 .0 9 7 0 .0 6 3 0 .0 3 0 0 .0 2 0
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage cholestero l (m M )
group 3 0 .0 0 0 0 .0 2 2 0 .0 2 2 0 .1 0 8 0 .4 1 2 0 .3 5 8 0 .2 2 4 0 .1 8 2 0 .1 5 0 0 .2 2 4 0 .3 2 8 0 .4 9 2 0 .5 9 6 0 .5 4 6 0 .4 7 4 0 .3 7 6 0 .4 6 2 0 .6 2 0 0 .5 7 6 0 .3 4 0 0 .1 9 4 0 .1 2 6 0 .0 6 0 0 .0 4 0
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured cholesterol (m M )
pool group 4
0.015 0.000 0.006 0.021 0.061 0.052 0.023 0.021 0.012 0.035 0.044 0.118 0.271 0.357 0.363 0.280 0.205 0.162 0.107 0.067 0.044 0.023 0.018 0.015
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A ve ra g e cholesterol (m M )
group 4
0.030 0.001 0.012 0.042 0.122 0.104 0.046 0.042 0.024 0.070 0.088 0.236 0.542 0.714 0.726 0.560 0.410 0.324 0.214 0.134 0.088 0.046 0.036 0.030
m ea su re d cholesterol (m M )
pool group 5
0.000 0.000 0.006 0.041 0.153 0.130 0.078 0.058 0.049 0.058 0.098 0.159 0.210 0.225 0.208 0.179 0.199 0.257 0.222 0.144 0.075 0.052 0.029 0.029
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (m M )
group 5
0.000 0.000 0.012 0.082 0.306 0.260 0.156 0.116 0.098 0.116 0.196 0.318 0.420 0.450 0.416 0.358 0.398 0.514 0.444 0.288 0.150 0.104 0.058 0.058
m easured cholesterol (m M )
pool group 6
0.000 0.006 0.009 0.035 0.107 0.095 0.061 0.044 0.038 0.032 0.067 0.150 0.265 0.372 0.377 0.300 0.239 0.196 0.150 0.092 0.052 0.032 0.021 0.021
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A v e ra g e cholesterol (m M )
group 6
0.001 0.012 0.018 0.070 0.214 0.190 0.122 0.088 0.076 0.064 0.134 0.300 0.530 0.744 0.754 0.600 0.478 0.392 0.300 0.184 0.104 0.064 0.042 0.042
m easured cholesterol (m M )
pool group 7
0.000 0.000 0.003 0.041 0.167 0.135 0.073 0.056 0.038 0.050 0.079 0.170 0.302 0.419 0.419 0.328 0.246 0.199 0.138 0.085 0.053 0.035 0.023 0.011
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (m M )
group 7
0.000 0.000 0.006 0.082 0.334 0.270 0.146 0.112 0.076 0.100 0.158 0.340 0.604 0.838 0.838 0.656 0.492 0.398 0.276 0.170 0.106 0.070 0.046 0.022
Page A22 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
EL
fractio n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m easured P L (m g/dl) pool group 1
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 2 .0 6 7 1.800 0.601 0.201 0 .3 3 4 1.400 3 .2 6 6 6 .5 9 9 8 .9 9 8 8 .7 3 2 7 .1 3 2 7 .2 6 5 11.931 2 0 .5 9 5 18.596 11.531 7 .2 6 5 4 .7 3 3 2 .4 6 7 0 .8 6 7
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage PL (mM ) group 1
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 3 2 0 .0 2 8 0 .0 0 9 0 .0 0 3 0 .0 0 5 0 .0 2 2 0 .0 5 0 0 .1 0 2 0 .1 3 9 0 .1 3 5 0 .1 1 0 0 .1 1 2 0 .1 8 4 0 .3 1 8 0 .2 8 7 0 .1 7 8 0 .1 1 2 0 .0 7 3 0 .0 3 8 0 .0 1 3
m easured P L (m g/dl) pool group 2
0 .0 0 0 0 .0 0 0 0 .0 0 0 0.201 3 .0 0 0 2 .0 6 7 0 .8 6 7 0.201 0 .3 3 4 1.000 2 .6 0 0 4 .9 9 9 6 .8 6 6 7 .9 3 2 7 .6 6 5 8 .0 6 5 10.731 15.130 12.864 8 .0 6 5 5 .2 6 6 3 .2 6 6 1.000 0 .0 0 0
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage PL (mM ) group 2
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 3 0 .0 4 6 0 .0 3 2 0 .0 1 3 0 .0 0 3 0 .0 0 5 0 .0 1 5 0 .0 4 0 0 .0 7 7 0 .1 0 6 0 .1 2 3 0 .1 1 9 0 .1 2 5 0 .1 6 6 0 .2 3 4 0 .1 9 9 0 .1 2 5 0.081 0 .0 5 0 0 .0 1 5 0 .0 0 0
m easured P L(m g/dl) pool group 3
0 .0 0 0 0 .0 0 0 0 .0 0 0 0 .0 0 0 2 .2 0 0 2 .0 6 7 0 .8 6 7 0 .3 3 4 0 .7 3 4 1.400 3 .5 3 3 6 .1 9 9 8 .1 9 9 8 .7 3 2 7 .2 6 5 7 .3 9 9 10.864 17.929 15.930 9 .7 9 8 6 .7 3 2 4 .2 0 0 1.534 0 .3 3 4
d ilu tio n
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
A verage PL (mM ) group 3
0.000 0.000 0.000 0.000 0.034 0 .0 3 2 0.013 0.005 0.011 0 .0 2 2 0.055 0.096 0 .1 2 7 0.135 0 .1 1 2 0.114 0.168 0 .2 7 7 0.246 0.151 0.104 0.065 0.024 0.005
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
m ea su re d PL(m g/dl) pool group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.048 2.583 8.185 12.587 12.987 10.453 8.052 6.184 4.183 2.583 1.916 1.115 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.040 0.127 0.195 0.201 0.162 0.124 0.096 0.065 0.040 0.030 0.017 0.000 0.000
m ea su re d PL(m g/dl) pool group 5
0.000 0.000 0.000 0.000 1.515 1.249 0.181 0.000 0.000 0.448 1.515 3.916 5.517 6.851 6.851 7.118 9.786 14.454 13.521 8.318 4.450 3.116 0.848 0.315
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 5
0.000 0.000 0.000 0.000 0.023 0.019 0.003 0.000 0.000 0.007 0.023 0.061 0.085 0.106 0.106 0.110 0.151 0.223 0.209 0.129 0.069 0.048 0.013 0.005
m easured PL(m g/dl) pool group 6
0.000 0.000 0.000 0.000 0.715 0.582 0.000 0.000 0.000 0.000 0.715 3.783 8.452 12.720 13.120 11.386 9.519 8.318 5.917 3.650 2.983 2.182 0.315 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 6
0.000 0.000 0.000 0.000 0.011 0.009 0.000 0.000 0.000 0.000 0.011 0.058 0.131 0.197 0.203 0.176 0.147 0.129 0.091 0.056 0.046 0.034 0.005 0.000
m easured PL(m g/dl) pool group 7
0.000 0.000 0.000 0.000 1.619 0.951 0.150 0.000 0.000 0.000 0.951 4.290 10.033 13.639 14.707 12.570 10.166 8.163 5.626 3.622 2.687 1.619 0.150 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (m M ) group 7
0.000 0.000 0.000 0.000 0.025 0.015 0.002 0.000 0.000 0.000 0.015 0.066 0.155 0.211 0.227 0.194 0.157 0.126 0.087 0.056 0.042 0.025 0.002 0.000
Page A23 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
C4. Pooled plasma ALAT measurements
group GGGGGGrrrrorrooooouuuuuuppGppppr647o523::::::uHHpHHHHFFFFFF1++:++++H000000F...0.0..000000c11233o6n%%%%%%troPPPPPPlFFFFFFOHBBOHSSSSSS
Pooled plasma ALAT (U/l)
week 0 mweeaesukr4ed dilution week 4 week 6 (o/n fast)
97.1 22381147380016....199756
3333333 289733161102536...778.3851
22311181401034367380
week 8 84331157579387.004402
Page A24 of A172
Appendix to TNO-report of 3M-02
Final version
0Q. 0Q. <<
C5. Plasma ketone bodies measurements
Week -1
concentration
ketonbodies (m m ol/l)
0 .0 0 0
0 .0 0 0 0.033 0.033 0.067 0.067 0.134 0.134 0.268 0.268 0.535 0.535 1 .0 7 0 1 .0 7 0 2.140 2.140
A 340 pre 0.214 0.214 0.218 0.215 0.218
0 .2 2 2 0.216
0.2 2 0.218 0.215 0.218 0.215 0.217 0.218 0.217 0.2 2
0 .2 1 1 0.214
0 .2 2 2
0.2 2 0.223 0.239 0.249 0.257 0.282 0.284 0.348 0.358 0.462 0.484 0.689 0.716
A 340 netto
-0.003 0
0.004 0.005 0.005 0.017 0.033 0.037 0.064 0.069 0 .1 3 0.143 0.245 0.266 0.472 0.496
y=ax + b
mouse#
641 645 646 650 652 655 656 657 658 663 665 666 667 670 671 674 678 679 680 681 686 687 690 696 699 700 704 705 706 710 712 714 716 720 721 724 727 729 730 731 737 738 740 741 742 744 748 752 754 756 758 759 760 761 762 763 765 766 768 769 771 774 776 778 782 783 784 785 790 791 792 793 795 797 799
a: | 0.2283 ~ | b: |
0.0028
A 340 pre
0.307 0.321
0.3 0.269 0.293 0.329 0.307 0.381 0.293 0 .3 5 0.245 0.276 0.273 0.351 0.324 0.464 0.291 0.447 0.334 0.468 0.352 0.467 0.356 0.274
0.3 0.305 0.311 0.294 0.292 0.419 0.388 0.289 0.333 0.346 0.302 0.347 0 .2 9 0.244 0.317 0 .2 7 0.432 0.387 0.351 0.249 0.239 0.242 0 .2 6 0.284 0.366 0.282 0.333 0.317 0.303 0.293 0.277 0.297 0.287 0.309 0.383 0.382 0.318 0.357 0.31 0.241 0.298 0.282 0.284 0.272 0.253 0.326 0.292 0.261 0.371 0.339 0 .30 1
0.766 0.546 0.623 0.644 0.546 0 .5 3 0.505 0 .6 7 0.658 0 .5 8 0.489 0 .6 5
0 .6 6 8 0.621 0.613 0.628 0.534 0.649 0.579 0.736 0.608 0.638 0.644 0.546 0.563 0.583
0 .6 6 6 0.609 0.621 0.664 0.624 0.573 0.465 0.692 0.705 0.754 0 .4 4 0 .3 7 0.423 0.405 0.712 0.581 0.578 0.485 0.418 0.516 0.429 0.445 0.631 0.558 0.664 0.613 0.605 0.639 0.623 0 .6 9 0 .6 2 0.627 0.632 0.693 0.533 0.554 0.521 0.466 0.596 0.556 0.653 0.645 0.452 0.589 0 .5 8 0.403 0.561 0.712 0 .57 2
A 340 netto
0.459 0.225 0.323 0.375 0.253
0 .2 0 1 0.198 0.289 0.365 0 .2 3 0.244 0.374 0.395 0 .2 7 0.289 0.164 0.243
0 .2 0 2 0.245 0.268 0.256 0.171 0.288 0.272 0.263 0.278 0.355 0.315 0.329 0.245 0.236 0.284 0.132 0.346 0.403 0.407 0 .1 5 0.126 0.106 0.135 0 .2 8 0.194 0.227 0.236 0.179 0.274 0.169 0.161 0.265 0.276 0.331 0.296 0.302 0.346 0.346 0.393 0.333 0.318 0.249 0.311 0.215 0.197
0 .2 1 1 0.225 0.298 0.274 0.369 0.373 0.199 0.263 0.288 0.142 0 .1 9 0.373 0.271
meas. concentration ketonbodies (m m ol/l)
2 .0 0 0.97 1 .4 0 1 .6 3 1.1 0 0.87
0 .8 6 1 .2 5 1 .5 9 1.0 0 1 .0 6 1 .6 3 1 .7 2 1 .1 7 1 .2 5 0.71 1 .0 5 0.87 1 .0 6 1 .1 6 1 .1 1 0.74 1 .2 5 1 .1 8 1 .1 4 1 .2 1 1 .5 4 1 .3 7 1 .4 3 1 .0 6
1.0 2 1 .2 3 0.57 1 .5 0 1 .7 5 1 .7 7 0.64 0.54 0.45 0.58 1 .2 1 0.84 0.98 1.0 2 0.77 1 .1 9 0.73 0.69 1 .1 5 1.2 0 1 .4 4 1 .2 8 1.31 1 .5 0 1 .5 0 1.71 1 .4 5 1 .3 8 1 .0 8 1 .3 5 0.93 0.85 0.91 0.97 1 .2 9 1 .1 9 1 .6 0 1 .6 2 0 .8 6 1 .1 4 1 .2 5 0.61 0.82 1 .6 2 1 .1 7
dilution
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1
Plasma keton bodies (m mol/l)
2.0 0 0 .9 7 1.40 1.63 1 .1 0 0 .8 7
0.8 6 1.25 1.59 1 .0 0 1.06 1.63 1.72 1.17 1.25 0.71 1.05 0 .8 7 1.06 1.16 1.11 0 .7 4 1.25 1.18 1.14 1.21 1.54 1.37 1.43 1.06
1 .0 2 1.23 0 .5 7 1.50 1.75 1.77 1.29 1.08 0 .9 0 1.16 1.21 0 .8 4 0 .9 8 1 .0 2 0 .7 7 1.19 0 .7 3 1.39 1.15 1 .2 0 1.44 1.28 1.31 1.50 1.50 1.71 1.45 1.38 1.08 1.35 0 .9 3 0 .8 5 0.91 0 .9 7 1.29 1.19 1.60 1.62 1.72 1.14 1.25 0.61 0 .8 2 1.62 1.17
mouse# after matching
11
12 13
21 61 excluded excluded 62
1
2 41 42 43 63 44 14 22 45 64 31 32 65 23
66 24 46 33 34 35 36 3 51 4 67 52
68 53 25 47 48 15 16 17 excluded excluded 49 excluded 5 26 27
6 28 18 69 70 7 19 29 37 54 38 55 56 57 58 59
8
20 9
10 39 30 40 60 50
04-12-2006
Page A25 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Week 6
concentration
odies (m mol/l)
0.000 0.000 0.033 0.033 0.067 0.067 0.134 0.134 0.268 0.268 0.535 0.535 1.070 1.070 2.140 2.140
A340 pre 0.255 0.254 0.255 0.258 0.256 0.262 0.257 0.262 0.257 0.256 0.258 0.257 0.256 0.261 0.259 0.266
A340 post
0.242 0.246 0.251 0.254 0.261 0.269 0.279 0.287 0.318 0.319 0.388 0.392
0.5 0.521 0.729 0.768
A340 netto
-0 .0 1 3 -0 .0 0 8 -0 .0 0 4 -0 .0 0 4 0.005 0.007 0.022 0.025 0.061 0.063
0.13 0.135 0.244 0.26 0.47 0.502
Keton bodies (mmol/l)
y=ax + b
Group
1 1 1 1 1
AVERAGE SD 2 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3 3
AVERAGE SD 4 4 4 4 4 4 4 4 4 4
AVERAGE SD 5 5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6 6
AVERAGE SD 7 7 7 7 7 7 7 7 7 7
AVERAGE SD
a: | 0.233 |
Mouse# cage#
11 21 32 42 53 63 74 85 95 10 5
b: | -0.00531
eartag
L
0 1 0 2 0 0 1 0 0 1
R
1 2 0 0 0 2 1 0 2 2
A340 pre
0.303 0.284 0.273 0.271 0.267 0.27 0.283 0.27 0.269 0.28
11 6
1 0 0.289
12 6
2 1 0.288
13 6
0 2 0.273
14 7
0 1 0.288
15 8
1 0 0.282
16 8
0 1 0.299
17 8
2 0 0.308
18 9
0 0 0.271
19 9
2 1 0.267
20 10 1 0 0.27
21 11 0 1 0.279 22 12 0 2 0.277 23 13 0 1 0.318 24 13 1 1 0.291 25 14 1 0 0.262 26 15 0 1 0.272 27 15 2 0 0.261 28 15 1 2 0.269 29 16 0 2 0.28 30 17 1 0 0.269
31 18 1 0 0.262
32 18 0 2 0.266
33 19 0 0 0.275
34 19 1 0 0.275
35 19 0 1 0.285
36 19 0 2 0.284
37 20 0 0 0.263
38 20 1 1 0.263
39 21 0 0 0.272
40 21
1 1 0.273
41 22
1 0 0.259
42 22 0 1 0.29
43 22 1 1 0.299
44 22
1 2 0.284
45 23
1 2 0.315
46 24 2 0 0.305
47 25 0 1 0.309
48 25 1 1 0.295
49 26 0 0 0.253
50 27
1 2 0.296
51 28 0 1 0.263
52 29
1 0 0.261
53 29
1 2 0.276
54 30
1 0 0.262
55 30 0 2 0.262
56 31 0 0 0.278
57 31 0 1 0.264
58 31 0 2 0.274
59 31 2 2 0.283
60 32 2 1 0.284
61 33 2 0 0.266
62 34
1 0 0.269
63 35 0 2 0.268
64 36 0 0 0.268
65 36
1 2 0.268
66 37 0 0 0.27
67 38 0 0 0.268
68 38 2 0 0.269
69 39
1 0 0.277
70 39 0 1 0.274
A340 post 0.443 0.402 0.383 0.359 0.382 0.363 0.424 0.38 0.367 0.379
0.413 0.343 0.382 0.468 0.395 0.347 0.364 0.413 0.366 0.383
0.443 0.473 0.409 0.38 0.421 0.418 0.419 0.374 0.445 0.372
0.409 0.381 0.424 0.439 0.452 0.446 0.417 0.365 0.49 0.427
0.435 0.437 0.541 0.408 0.558 0.529 0.399 0.439 0.44 0.466
0.581 0.556 0.541 0.54 0.551 0.605 0.408 0.605 0.564 0.659
0.551 0.568 0.566 0.522 0.541 0.579 0.615 0.568 0.618 0.647
A340 netto
0.14 0.118 0.11 0.088 0.115 0.093 0.141 0.11 0.098 0.099
0.124 0.055 0.109 0.18 0.113 0.048 0.056 0.142 0.099 0.113
0.164 0.196 0.091 0.089 0.159 0.146 0.158 0.105 0.165 0.103
0.147 0.115 0.149 0.164 0.167 0.162 0.154 0.102 0.218 0.154
0.176 0.147 0.242 0.124 0.243 0.224 0.09 0.144 0.187 0.17
0.318 0.295 0.265 0.278 0.289 0.327 0.144 0.331 0.281 0.375
0.285 0.299 0.298 0.254 0.273 0.309 0.347 0.299 0.341 0.373
meas. concentration ketonbodies (mmol/l)
0.62 0.53 0.49 0.40 0.52 0.42 0.63 0.49 0.44 0.45
0.55 0.26 0.49 0.80 0.51 0.23 0.26 0.63 0.45 0.51
0.73 0.86 0.41 0.40 0.71 0.65 0.70 0.47 0.73 0.46
0.65 0.52 0.66 0.73 0.74 0.72 0.68 0.46 0.96 0.68
0.78 0.65 1.06 0.55 1.07 0.98 0.41 0.64 0.83 0.75
1.39 1.29 1.16 1.22 1.26 1.43 0.64 1.44 1.23 1.63
1.25 1.31 1.30 1.11 1.19 1.35 1.51 1.31 1.49 1.62
dilution
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
Plasma keton
bodies (mmol/l)
1.87 1.59 1.48 1.20 1.55 1.27 1.88 1.48 1.33 1.34 1.50 0.23
1.66 0.78 1.47 2.39 1.52 0.69 0.79 1.90 1.34 1.52
1.41 0.54 2.18 2.59 1.24 1.21 2.12 1.95 2.10 1.42 2.19 1.39 1.84 0.48 1.96 1.55 1.99 2.18 2.22 2.15 2.05 1.38 2.88 2.05 2.04 0.40
2.33 1.96 3.18 1.66 3.20 2.95 1.23 1.92 2.48 2.26
2.32 0.65 4.16 3.87 3.48 3.65 3.79 4.28 1.92 4.33 3.69 4.90 3.81 0.78
3.74 3.92 3.91 3.34 3.58 4.05 4.54 3.92 4.46 4.87
4.03 0.47
Page A26 of A172
Appendix to TNO-report of 3M-02
Final version
G roup
M ouse# cage#
i i 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD
3 3 3 3 3 3 3 3 3 3 AVERAGE SD
4 4 4 4 4 4 4 4 4 4
AVERAGE SD 5 5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6 6
AVERAGE SD 7 7 7 7 7 7 7 7 7 7
AVERAGE SD
e x c lu d e d (655) e x c lu d e d (656) e x c lu d e d (741) e x c lu d e d (7 4 2 ) e x c lu d e d (748)
i 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
1 1 2 2 3 3 4 5 5 5
6 6 6 7 8 8 8 9 9 10
11 12 13 13 14 15 15 15 16 17
18 18 19 19 19 19 20 20 21 21
22 22 22 22 23 24 25 25 26 27
28 29 29 30 30 31 31 31 31 32
33 34 35 36 36 37 38 38 39 39
earta g LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
1 P la sm a k e to n b o d ie s (m m o l/l) 1
w e e k -1
week 6
1.59 1.00 1.02 0 .5 7 1.39 1.44 1.71 1.60 1.72 1.14
1.87 1.59 1.48 1.20 1.55 1.27 1.88 1.48 1.33 1.34
1.32 0 .3 8
1.50 0 .2 3
2 .0 0
1.66
0 .9 7 1.40 0.71 1.21 0 .8 4 0 .9 8 1.31 1.45 1.62
0 .7 8 1.47 2 .3 9 1.52 0 .6 9 0 .7 9 1.90 1.34 1.52
1.25 0 .3 9
1.41 0 .5 4
1.63 1.05 1.25 1.14 1.08 1.15 1.20 1.28 1.38 0.61
2 .1 8 2 .5 9 1.24 1.21 2 .1 2 1.95 2 .1 0 1.42 2 .1 9 1.39
1.18 0 .2 6
1.84 0 .4 8
1.16 1.11 1.54 1.37 1.43 1.06 1.08
1.96 1.55 1.99 2 .1 8 2 .2 2 2 .1 5 2 .0 5
0 .9 3 1.25 0 .8 2
1.38 2 .8 8 2 .0 5
1.17 0 .2 2
2 .0 4 0 .4 0
1.06 1.63 1.72 1.25 0 .8 7 1.21 0 .9 0 1.16 1.19 1.17
2 .3 3 1.96 3 .1 8 1.66 3 .2 0 2 .9 5 1.23 1.92 2 .4 8 2 .2 6
1.22 0 .2 7
2 .3 2 0 .6 5
1.23 1.75 1.29 1.35 0 .8 5
4 .1 6 3 .8 7 3 .4 8 3 .6 5 3 .7 9
0.91 0 .9 7 1.29 1.19 1.62
4 .2 8 1.92 4 .3 3 3 .6 9 4 .9 0
1.25 0 .2 9
3.81 0 .7 8
1.10 1.25 1.17 1.06 0 .7 4 1.18 1.50 1.77 1.50 1.50
3 .7 4 3 .9 2 3.91 3 .3 4 3 .5 8 4 .0 5 4 .5 4 3 .9 2 4 .4 6 4 .8 7
1.28 0 .3 0
4 .0 3 0 .4 7
0 .8 7 0 .8 6 1.02 0 .7 7
0 .7 3
04-12-2006
Appendix to TNO-report of 3M-02
Final version
Appendix D. Measurements in metabolic cages
D1. Code mice and metabolic cages
group# mouse# metabolic cage#
12 13 11 46 3 21 3 22 33 2247 4 31 4 32 4 37 4 38 6 51 6 53 6 54 6 55
101 105 220015 102 106 220026 103 107 203 207 104 108 204 208
04-12-2006
D2. Average bodyweight mice in metabolic cages
group# mouse# metabolic cage#
Bodyweight (g)
Average bodyweight (g)
begin metabolic cage end metabolic cage in metabolic cage
12
101
29.9
30.7
30.30
13
105
30.2
30
30.10
14
201
28
29.1
28.55
16
205
29.3
29.6
29.45
3 21
102
29.5
30.2
29.85
3 22
106
29.8
30.1
29.95
33 2247
220026
2289..85
2390..72
2299..8255
4 31
103
28.9
29.8
29.35
4 32
107
28.9
29
28.95
4 37
203
26.9
28.9
27.90
4 38
207
30.4
31.3
30.85
6 51
104
28.2
29.1
28.65
6 53
108
27.3
26.5
26.90
66 5554
220048
2297..48
2380..87
2380..3005
Page A28 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
D3. Food and drink intake measurements
Food intake measurements
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1: HF control
Accumulated food intake (g)
Average acc.
cage 0101 cage 0105 cage 0201 cage 0205 fo o d in ta k e ( g )
0.258
0.182
0.114
0.206
0.19
0.735
0.468
0.273
0.555
0.51
0.888
0.696
0.733
0.777
0.77
1.017
0.851
0.926
0.997
0 .9 5
1.205
0.946
1.021
1.184
1.09
1.380
1.248
1.356
1.345
1.33
1.571
1.388
1.770
1.525
1.56
1.810
1.510
2.016
1.681
1.75
2.015
1.682
2.182
1.792
1.92
2.184
1.747
2.294
1.898
2.03
2.402
1.837
2.324
1.992
2 .1 4
2.458
1.940
2.623
2.060
2.27
2.616
2.045
3.224
2.152
2.51
2.724
2.188
3.390
2.308
2 .6 5
2.824
2.437
3.480
2.473
2.80
3.064
2.599
3.486
2.689
2.96
3.300
2.792
3.510
2.748
3.09
3.564
3.013
3.525
2.995
3.27
3.879
3.109
3.676
3.304
3.49
4.146
3.214
3.802
3.448
3 .6 5
4.399
3.284
3.980
3.750
3 .8 5
4.531
3.372
4.110
3.918
3.98
4.743
3.412
4.174
3.977
4 .0 8
4.856
3.514
4.280
4.085
4 .1 8
4.935
3.602
4.321
4.343
4 .3 0
5.129
3.739
4.441
4.488
4 .4 5
5.509
4.064
4.469
4.635
4 .6 7
5.770
4.341
4.705
4.815
4.91
5.968
4.531
4.897
4.947
5.09
6.096
4.684
5.032
5.059
5.22
6.260
4.869
5.144
5.138
5 .3 5
6.378
5.016
5.351
5.318
5.52
6.529
5.181
5.535
5.470
5.68
6.741
5.397
5.692
5.729
5.89
6.916
5.608
5.944
5.965
6.11
7.046
5.794
6.060
6.139
6.26
7.233
5.993
6.328
6.449
6.50
7.443
6.101
6.615
6.749
6.73
7.561
6.231
6.776
6.879
6.86
7.629
6.317
7.002
7.007
6.99
7.763
6.406
7.175
7.153
7.12
8.003
6.555
7.392
7.280
7.31
8.140
6.690
7.504
7.345
7.42
8.297
6.796
7.656
7.432
7 .5 5
8.485
6.840
7.831
7.553
7.68
8.671
7.034
7.922
7.620
7.81
8.766
7.102
7.973
7.671
7.88
8.926
7.156
8.014
7.735
7.96
9.030
7.190
8.075
7.826
8.03
9.096
7.277
8.149
7.856
8.09
9.128
7.336
8.299
7.904
8.17
9.139
7.553
8.481
8.007
8.30
9.279
7.633
8.613
8.157
8.42
9.441
7.933
8.781
8.277
8.61
9.564
8.168
8.938
8.488
8.79
9.710
8.462
9.207
8.706
9.02
9.835
8.621
9.459
8.970
9.22
10.044 8.689
9.575
9.184
9.37
SD (g ) 0 .0 6 0 .1 9 0 .0 8 0 .0 8 0 .1 3 0 .0 6 0 .1 6 0.21 0 .2 2 0 .2 5 0 .2 7 0 .3 2 0 .5 4 0 .5 4 0 .4 8 0 .4 0 0 .3 8 0.31 0 .3 5 0.41 0 .4 6 0 .4 8 0 .5 5 0 .5 5 0 .5 5 0 .5 7 0.61 0.61 0 .6 2 0.61 0 .6 2 0 .5 9 0 .5 9 0 .5 9 0 .5 6 0 .5 4 0 .5 2 0 .5 5 0 .5 5 0 .5 4 0 .5 6 0 .5 9 0 .6 0 0 .6 2 0 .6 8 0 .6 8 0 .6 9 0 .7 4 0 .7 6 0 .7 6 0 .7 5 0 .6 8 0 .7 0 0 .6 6 0.61 0 .5 5 0 .5 3 0 .5 8
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
Accumulated food intake (g)
Average acc.
cage 0102 cage 0106 cage 0202 cage 0206 fo o d in ta k e ( g )
0.215
0.190
0.188
0.203
0 .2 0
0.548
0.528
0.643
0.494
0 .5 5
0.834
0.665
0.961
0.615
0 .7 7
0.979
0.822
1.200
0.745
0 .9 4
1.156
1.081
1.312
0.890
1.11
1.416
1.340
1.505
1.058
1.33
1.624
1.437
1.738
1.282
1.52
1.828
1.652
1.911
1.374
1.69
2.005
1.696
2.105
1.503
1.83
2.164
1.772
2.282
1.601
1 .9 5
2.200
1.844
2.450
1.658
2 .0 4
2.290
1.877
2.731
1.761
2 .1 6
2.416
2.008
2.955
1.807
2 .3 0
2.506
2.171
3.097
1.885
2.41
2.638
2.304
3.284
2.120
2 .5 9
2.790
2.564
3.633
2.294
2 .8 2
3.033
2.637
3.819
2.454
2 .9 9
3.288
2.836
4.141
2.726
3 .2 5
3.478
3.028
4.557
2.931
3 .5 0
3.658
3.030
4.845
3.087
3 .6 6
3.815
3.033
4.958
3.251
3 .7 6
3.989
3.083
5.184
3.466
3 .9 3
4.160
3.140
5.331
3.592
4 .0 6
4.264
3.200
5.478
3.741
4 .1 7
4.385
3.322
5.596
3.824
4 .2 8
4.660
3.464
5.823
4.060
4 .5 0
5.208
3.675
6.138
4.316
4 .8 3
5.472
3.794
6.381
4.484
5 .0 3
5.665
4.016
6.645
4.648
5 .2 4
5.794
4.175
6.781
4.691
5 .3 6
5.890
4.375
6.887
4.736
5 .4 7
6.059
4.476
7.033
4.870
5.61
6.241
4.642
7.204
4.965
5 .7 6
6.399
4.839
7.392
5.180
5 .9 5
6.520
5.095
7.600
5.323
6 .1 3
6.764
5.403
7.862
5.512
6 .3 9
7.101
5.597
8.127
5.755
6 .6 5
7.424
5.802
8.354
6.043
6.91
7.608
5.958
8.528
6.120
7 .0 5
7.838
6.095
8.658
6.304
7 .2 2
8.024
6.240
8.807
6.420
7 .3 7
8.114
6.300
8.955
6.534
7 .4 8
8.228
6.388
9.050
6.656
7 .5 8
8.312
6.460
9.159
6.748
7 .6 7
8.479
6.546
9.299
6.817
7 .7 9
8.672
6.724
9.494
6.931
7 .9 6
8.711
6.908
9.698
6.987
8 .0 8
8.785
7.060
9.857
7.086
8 .2 0
8.830
7.178
9.942
7.105
8 .2 6
8.844
7.263 10.006 7.200
8 .3 3
8.903
7.332
10.105 7.265
8 .4 0
8.920
7.393 10.230 7.319
8 .4 7
8.996
7.574 10.390 7.448
8 .6 0
9.098
7.806 10.490 7.629
8 .7 6
9.384
7.976
10.763 7.816
8 .9 8
9.629
8.172 11.070 8.089
9 .2 4
9.819
8.314 11.308 8.285
9 .4 3
9.936
8.454 11.426 8.447
9 .5 7
SD (g ) 0.01 0 .0 6 0 .1 6 0 .2 0 0 .1 8 0 .1 9 0 .2 0 0 .2 4 0 .2 8 0 .3 2 0 .3 5 0 .4 4 0.51 0 .5 2 0.51 0 .5 8 0.61 0 .6 4 0 .7 4 0 .8 4 0 .8 6 0.91 0 .9 5 0 .9 7 0 .9 8 1.01 1.07 1.13 1.16 1.16 1 .1 4 1.16 1.18 1.17 1.16 1.16 1.20 1.20 1.23 1.23 1 .2 5 1.27 1.27 1.28 1.32 1 .3 5 1.36 1.37 1.37 1 .3 5 1.36 1.39 1.38 1.33 1.38 1.41 1 .4 4 1.42
Page A29 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Metaboliccagesgroup4: HF+0.006%PFHS
Time Accumulatedfoodintake(g) Average acc.
(hr) cage0103 cage0107 cage0203 cage0207 food intake (g)
6.1 0.211 0.360 0.336 0.424
0.33
7.4 0.535 0.688 0.784 0.907
0.73
8.6 0.656 0.881 1.069 1.157
0.94
9.9 0.844 1.075 1.187 1.292
1.10
11.1 0.952 1.146 1.318 1.510
1.23
12.4 1.045 1.282 1.490 1.612
1.36
13.6 1.181 1.410 1.611 1.682
1.47
14.9 1.355 1.470 1.719 1.825
1.59
16.1 1.475 1.518 1.796 1.867
1.66
17.4 1.612 1.603 1.896 2.072
1.80
18.6 1.724 1.856 2.060 2.173
1.95
19.9 1.840 2.142 2.138 2.328
2.11
21.1 2.066 2.412 2.257 2.463
2.30
22.4 2.272 2.630 2.440 2.629
2.49
23.6 2.408 2.775 2.575 2.771
2.63
24.9 2.588 2.822 2.849 2.957
2.80
26.1 2.702 2.952 3.090 3.204
2.99
27.4 2.899 3.350 3.486 3.580
3.33
28.6 3.247 3.880 3.988 4.074
3.80
29.9 3.592 4.090 4.394 4.524
4.15
31.1 3.788 4.209 4.849 4.851
4.42
32.4 3.849 4.315 5.167 5.095
4.61
33.6 3.935 4.435 5.255 5.180
4.70
34.9 4.033 4.530 5.345 5.222
4.78
36.1 4.112 4.596 5.513 5.308
4.88
37.4 4.297 4.917 5.702 5.423
5.08
38.6 4.563 5.184 5.847 5.572
5.29
39.9 4.733 5.370 5.977 5.836
5.48
41.1 4.925 5.644 6.195 5.974
5.68
42.4 5.058 5.862 6.305 6.104
5.83
43.6 5.163 6.025 6.418 6.208
5.95
44.9 5.285 6.249 6.561 6.264
6.09
46.1 5.412 6.429 6.782 6.450
6.27
47.4 5.592 6.682 7.023 6.601
6.47
48.6 5.945 6.866 7.303 6.770
6.72
49.9 6.233 7.064 7.717 6.965
6.99
51.1 6.686 7.269 8.032 7.365
7.34
52.4 7.036 7.546 8.318 7.866
7.69
53.6 7.312 7.920 8.558 8.118
7.98
54.9 7.437 8.042 8.678 8.400
8.14
56.1 7.544 8.134 8.803 8.589
8.27
57.4 7.578 8.166 8.931 8.735
8.35
58.6 7.667 8.205 9.030 8.832
8.43
59.9 7.792 8.260 9.197 8.900
8.54
61.1 7.871 8.338 9.339 9.022
8.64
62.4 7.998 8.416 9.506 9.156
8.77
63.6 8.093 8.503 9.620 9.257
8.87
64.9 8.272 8.585 9.680 9.388
8.98
66.1 8.345 8.669 9.753 9.484
9.06
67.4 8.426 8.750 9.904 9.569
9.16
68.6 8.474 8.848 10.001 9.658
9.25
69.9 8.596 8.900 10.123 9.772
9.35
71.1 8.664 9.017 10.340 9.883
9.48
72.4 8.858 9.148 10.445 10.118
9.64
73.6 9.226 9.256 10.689 10.283
9.86
74.9 9.590 9.385 10.994 10.529
10.12
76.1 9.961 9.515 11.208 10.865
10.39
77.4 10.204 9.712 11.433 11.104
10.61
SD (g) 0.09 0.16 0.22
0.19 0.24 0.25 0.23
0.22 0.20 0.23 0.20
0.20 0.18 0.17 0.18
0.16 0.22 0.30 0.38 0.41 0.52 0.64 0.63 0.62 0.65 0.62 0.56 0.56 0.55 0.55 0.55 0.56 0.59 0.62 0.57 0.61 0.55 0.54 0.52 0.54 0.56 0.61 0.62 0.63 0.66 0.69 0.70 0.66 0.66 0.69 0.71 0.72 0.77 0.76 0.74 0.76 0.78 0.79
Metaboliccagesgroup6 HF+0.003%PFOS
Time
Accumulatedfoodintake(g)
| Average acc.
(hr) cage0104cage0108 cage0204cage0208 food intake (g)
6.1 0.204 0.119 0.211 0.256
0.20
7.4 0.535 0.170 0.629 0.547
0.47
8.6 0.808 0.260 0.916 0.709
0.67
9.9 0.960 0.518 1.134 0.865
0.87
11.1 1.185 0.732 1.406 1.047
1.09
12.4 1.512 0.847 1.618 1.189
1.29
13.6 1.749 0.889 1.848 1.340
1.46
14.9 1.940 0.998 2.001 1.406
1.59
16.1 2.020 1.095 2.100 1.591
1.70
17.4 2.174 1.216 2.185 1.642
1.80
18.6 2.406 1.304 2.252 1.767
1.93
19.9 2.543 1.370 2.270 1.851
2.01
21.1 2.648 1.558 2.390 1.938
2.13
22.4 2.810 1.650 2.480 2.082
2.26
23.6 3.050 1.750 2.546 2.234
2.40
24.9 3.301 1.898 2.689 2.577
2.62
26.1 3.423 2.071 2.851 2.815
2.79
27.4 3.778 2.310 3.211 3.149
3.11
28.6 4.101 2.641 3.553 3.436
3.43
29.9 4.314 2.965 3.990 3.637
3.73
31.1 4.529 3.048 4.316 3.864
3.94
32.4 4.663 3.078 4.572 3.978
4.07
33.6 4.720 3.203 4.695 4.070
4.17
34.9 4.865 3.307 5.002 4.232
4.35
36.1 4.989 3.385 5.161 4.322
4.46
37.4 5.264 3.615 5.358 4.501
4.68
38.6 5.554 3.670 5.466 4.702
4.85
39.9 5.835 3.735 5.640 4.919
5.03
41.1 6.067 3.862 5.747 5.126
5.20
42.4 6.303 3.946 5.878 5.205
5.33
43.6 6.441 4.055 6.075 5.268
5.46
44.9 6.584 4.147 6.188 5.421
5.59
46.1 6.733 4.293 6.320 5.543
5.72
47.4 7.107 4.400 6.508 5.644
5.91
48.6 7.672 4.522 6.617 5.846
6.16
49.9 7.929 4.758 6.854 6.035
6.39
51.1 8.198 5.100 7.194 6.268
6.69
52.4 8.427 5.352 7.512 6.465
6.94
53.6 8.566 5.511 7.721 6.688
7.12
54.9 8.762 5.603 7.901 6.921
7.30
56.1 8.892 5.689 8.062 7.030
7.42
57.4 9.088 5.749 8.139 7.093
7.52
58.6 9.207 5.852 8.244 7.166
7.62
59.9 9.378 6.024 8.302 7.200
7.73
61.1 9.554 6.105 8.386 7.307
7.84
62.4 9.689 6.228 8.526 7.433
7.97
63.6 9.839 6.294 8.674 7.524
8.08
64.9 10.003 6.361 8.830 7.611
8.20
66.1 10.139 6.478 8.880 7.664
8.29
67.4 10.256 6.542 8.933 7.717
8.36
68.6 10.347 6.591 9.006 7.720
8.42
69.9 10.464 6.718 9.083 7.740
8.50
71.1 10.593 6.810 9.309 7.807
8.63
72.4 10.793 6.915 9.451 7.914
8.77
73.6 11.011 7.103 9.579 8.188
8.97
74.9 11.264 7.345 9.788 8.977
9.34
76.1 11.518 7.679 10.107 9.184
9.62
77.4 11.641 7.878 10.312 9.426
9.81
SD (g) 0.06 0.20 0.29
0.26 0.28 0.35 0.44
0.47 0.46 0.47 0.50
0.51 0.48 0.50 0.55
0.57 0.55 0.60 0.60 0.58 0.66 0.73 0.71 0.77 0.81 0.81 0.87 0.95 0.97 1.03 1.06 1.07 1.07 1.17 1.33 1.34 1.32 1.33 1.32 1.36 1.38 1.43 1.44 1.44 1.48 1.48 1.52 1.57 1.58 1.60 1.62 1.63 1.66 1.71 1.70 1.63 1.61 1.58
Page A30 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time ( hr)
6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p
4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000 4 4 1.000 4 4 1.000 4 4 1.000 4 4 1.000 4 4 0.860 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.886 4 4 1.000 4 4 1.000 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886
Accumulated Food intake; p-values Mann Whitney U-test
group 1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6
n n p nn
n
nn
4 0.057 4 0.860
0.057 4
0 .6 8 6
0 .0 5 7
4 0.200 4 0.886
0.114 4
0 .8 8 6
0 .1 1 4
4 0.486 4 1.000
0.343 4
0 .8 8 6
0 .3 4 3
4 0.343 4 0.886
0.343 4
0 .8 8 6
0 .3 4 3
4 0.486 4 0.886
0.486 4
1.000
0 .6 8 6
4 0.886 4 1.000
1.000 4
0 .8 8 6
1.000
4 0.886 4 0.886
0.686 4
0 .8 8 6
0 .8 8 6
4 0.486 4 0.686
0.486 4
0 .8 8 6
0 .8 8 6
4 0.343 4 0.486
0.486 4
0 .8 8 6
0 .6 8 6
4 0.200 4 0.486
0.486 4
0 .8 8 6
0 .6 8 6
4 0.486 4 0.686
0.886 4
0 .8 8 6
0 .8 8 6
4 0.686 4 0.486
1.000 4
0 .6 8 6
1.000
4 0.886 4 0.486
0.886 4
0 .6 8 6
0 .6 8 6
4 0.886 4 0.486
0.686 4
0 .6 8 6
0 .6 8 6
4 0.686 4 0.486
0.686 4
0 .6 8 6
0 .4 8 6
4 0.686 4 0.343
0.486 4
0 .8 8 6
0 .4 8 6
4 0.686 4 0.886
0.686 4
0 .8 8 6
0 .6 8 6
4 1.000 4 1.000
0.486 4
0 .8 8 6
0 .6 8 6
4 0.200 4 1.000
0.486 4
1.000
0 .6 8 6
4 0.200 4 0.886
0.486 4
1.000
0 .3 4 3
4 0.200 4 0.886
0.486 4
0 .6 8 6
0 .4 8 6
4 0.343 4 0.686
0.486 4
0 .8 8 6
0 .4 8 6
4 0.343 4 1.000
0.486 4
0 .8 8 6
0 .4 8 6
4 0.343 4 0.686
0.486 4
0 .8 8 6
0 .4 8 6
4 0.343 4 0.686
0.486 4
0 .8 8 6
0 .4 8 6
4 0.343 4 0.486
0.486 4
0 .8 8 6
0 .4 8 6
4 0.200 4 0.686
0.686 4
1.000
0 .4 8 6
4 0.200 4 0.686
0.686 4
1.000
0 .4 8 6
4 0.200 4 0.686
0.686 4
1.000
0 .6 8 6
4 0.200 4 0.686
0.486 4
1.000
0 .6 8 6
4 0.200 4 0.686
0.486 4
1.000
0 .8 8 6
4 0.486 4 0.686
0.486 4
1.000
0 .6 8 6
4 0.486 4 0.686
0.486 4
1.000
0 .4 8 6
4 0.486 4 1.000
0.486 4
1.000
0 .6 8 6
4 0.343 4 1.000
0.486 4
0 .8 8 6
0 .4 8 6
4 0.114 4 1.000
0.486 4
0 .8 8 6
0 .4 8 6
4 0.057 4 1.000
0.486 4
0 .8 8 6
0 .4 8 6
4 0.057 4 0.886
0.486 4
0 .8 8 6
0 .4 8 6
4 0.057 4 0.886
0.343 4
0 .8 8 6
0 .4 8 6
4 0.057 4 0.886
0.343 4
0 .8 8 6
0 .4 8 6
4 0.057 4 0.886
0.486 4
0 .8 8 6
0 .4 8 6
4 0.057 4 0.886
0.486 4
0 .8 8 6
0 .4 8 6
4 0.057 4 0.886
0.686 4
0 .8 8 6
0 .6 8 6
4 0.114 4 0.886
0.486 4
1.000
0 .6 8 6
4 0.114 4 1.000
0.486 4
1.000
0 .6 8 6
4 0.114 4 1.000
0.486 4
1.000
0 .6 8 6
4 0.114 4 1.000
0.686 4
1.000
0 .6 8 6
4 0.114 4 1.000
0.686 4
0 .8 8 6
0 .6 8 6
4 0.114 4 1.000
0.686 4
0 .8 8 6
0 .6 8 6
4 0.114 4 1.000
0.686 4
0 .8 8 6
0 .6 8 6
4 0.114 4 1.000
0.686 4
0 .8 8 6
0 .6 8 6
4 0.114 4 1.000
0.686 4
0 .8 8 6
0 .6 8 6
4 0.114 4 0.886
0.486 4
0 .8 8 6
0 .6 8 6
4 0.114 4 1.000
0.486 4
0 .8 8 6
0 .6 8 6
4 0.114 4 0.886
0.686 4
0 .8 8 6
0 .6 8 6
4 0.114 4 0.686
0.686 4
0 .8 8 6
0 .6 8 6
4 0.057 4 0.686
0.486 4
0 .8 8 6
0 .6 8 6
4 0.057 4 0.686
0.343 4
0 .8 8 6
0 .6 8 6
p< 0 .0 5
Page A31 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Feeding bout size measurements
Metaboliccagesgroup1: HFcontrol
Time
Feedingboutsize(g)
Average feeding SD
J6M.1
cage0101 cage0105 cage0201 cage0205 bout size (g)
0.045 0.036 0.018 0.044
0.036
(g) 0.013
7.4 0.043 0.019 0.032 0.025
0.030
0.010
8.6 0.002 0.029 0.034 0.018
0.021
0.014
9.9 0.018 0.002 0.012 0.025
0.014
0.010
11.1 0.017 0.014 0.010 0.008
0.012
0.004
12.4 0.022 0.032 0.053 0.022
0.032
0.015
13.6 0.024 0.013 0.038 0.017
0.023
0.011
14.9 0.023 0.017 0.018 0.013
0.018
0.004
16.1 0.013 0.010 0.005 0.013
0.010
0.004
17.4 0.014 0.005 0.012 0.012
0.011
0.004
18.6 0.023 0.013 0.002 0.003
0.010
0.010
19.9 0.006 0.005 0.078 0.012
0.025
0.035
21.1 0.019 0.014 0.015 0.008
0.014
0.005
22.4 0.008 0.028 0.021 0.014
0.018
0.009
23.6 0.017 0.020 0.000 0.025
0.016
0.011
24.9 0.016 0.005 0.003 0.011
0.009
0.006
26.1 0.038 0.030 0.000 0.016
0.021
0.017
27.4 0.012 0.012 0.005 0.026
0.014
0.009
28.6 0.040 0.011 0.021 0.028
0.025
0.012
29.9 0.029 0.011 0.021 0.009
0.018
0.009
31.1 0.015 0.010 0.000 0.042
0.017
0.018
32.4 0.025 0.002 0.016 0.004
0.012
0.011
33.6 0.011 0.008 0.006 0.003
0.007
0.003
34.9 0.007 0.009 0.008 0.015
0.010
0.004
36.1 0.016 0.016 0.008 0.033
0.018
0.011
37.4 0.031 0.004 0.009 0.010
0.014
0.012
38.6 0.026 0.046 0.011 0.015
0.025
0.016
39.9 0.026 0.025 0.025 0.015
0.023
0.005
41.1 0.018 0.012 0.013 0.010
0.013
0.003
42.4 0.012 0.017 0.010 0.011
0.013
0.003
43.6 0.021 0.014 0.013 0.017
0.016
0.004
44.9 0.008 0.018 0.024 0.017
0.017
0.007
46.1 0.018 0.023 0.021 0.021
0.021
0.002
47.4 0.023 0.020 0.018 0.020
0.020
0.002
48.6 0.009 0.023 0.021 0.017
0.018
0.006
49.9 0.021 0.017 0.012 0.028
0.020
0.007
51.1 0.025 0.014 0.038 0.030
0.027
0.010
52.4 0.010 0.014 0.025 0.029
0.020
0.009
53.6 0.008 0.009 0.008 0.011
0.009
0.001
54.9 0.011 0.009 0.023 0.015
0.015
0.006
56.1 0.013 0.013 0.021 0.016
0.016
0.004
57.4 0.027 0.012 0.015 0.000
0.014
0.011
58.6 0.019 0.012 0.016 0.007
0.014
0.005
59.9 0.007 0.009 0.015 0.018
0.012
0.005
61.1 0.029 0.004 0.013 0.009
0.014
0.011
62.4 0.011 0.020 0.008 0.000
0.010
0.008
63.6 0.002 0.005 0.004 0.008
0.005
0.003
64.9 0.024 0.007 0.008 0.012
0.013
0.008
66.1 0.002 0.002 0.001 0.001
0.002
0.001
67.4 0.008 0.010 0.018 0.003
0.010
0.006
68.6 0.001 0.015 0.009 0.014
0.010
0.006
69.9 0.002 0.017 0.017 0.001
0.009
0.009
71.1 0.017 0.002 0.014 0.021
0.014
0.008
72.4 0.018 0.047 0.019 0.015
0.025
0.015
73.6 0.009 0.012 0.017 0.027
0.016
0.008
74.9 0.019 0.038 0.035 0.020
0.028
0.010
76.1 0.021 0.002 0.013 0.027
0.016
0.011
77.4 0.010 0.010 0.015 0.014
0.012
0.003
Metaboliccagesgroup3: HF+0.03% PFBS
Time
Feedingboutsize(g)
Average feeding SD
(hr) cage0102 cage0106 cage0202 cage0206 bout size (g) (g)
6.1 0.045 0.043 0.032 0.041
0.040
0.006
7.4 0.018 0.018 0.038 0.010
0.021
0.012
8.6 0.024 0.011 0.033 0.014
0.021
0.010
9.9 0.017 0.026 0.025 0.015
0.021
0.006
11.1 0.036 0.024 0.008 0.015
0.021
0.012
12.4 0.002 0.019 0.026 0.020
0.017
0.010
13.6 0.036 0.017 0.018 0.020
0.023
0.009
14.9 0.012 0.008 0.024 0.008
0.013
0.008
16.1 0.015 0.011 0.019 0.011
0.014
0.004
17.4 0.013 0.001 0.013 0.007
0.009
0.006
18.6 0.010 0.008 0.015 0.012
0.011
0.003
19.9 0.001 0.004 0.028 0.005
0.010
0.012
21.1 0.018 0.018 0.024 0.003
0.016
0.009
22.4 0.012 0.019 0.009 0.015
0.014
0.004
23.6 0.009 0.007 0.025 0.020
0.015
0.009
24.9 0.015 0.028 0.036 0.027
0.027
0.009
26.1 0.028 0.009 0.021 0.012
0.018
0.009
27.4 0.028 0.031 0.046 0.029
0.034
0.008
28.6 0.020 0.001 0.027 0.021
0.017
0.011
29.9 0.011 0.000 0.024 0.009
0.011
0.010
31.1 0.024 0.003 0.016 0.029
0.018
0.011
32.4 0.022 0.008 0.012 0.009
0.013
0.006
33.6 0.000 0.000 0.023 0.008
0.008
0.011
34.9 0.014 0.011 0.010 0.018
0.013
0.004
36.1 0.013 0.012 0.015 0.012
0.013
0.001
37.4 0.055 0.010 0.031 0.032
0.032
0.018
38.6 0.044 0.025 0.028 0.016
0.028
0.012
39.9 0.009 0.012 0.032 0.020
0.018
0.010
41.1 0.027 0.031 0.012 0.009
0.020
0.011
42.4 0.006 0.015 0.011 0.003
0.009
0.005
43.6 0.014 0.011 0.020 0.012
0.014
0.004
44.9 0.010 0.009 0.010 0.010
0.010
0.001
46.1 0.023 0.017 0.018 0.016
0.019
0.003
47.4 0.017 0.032 0.017 0.022
0.022
0.007
48.6 0.005 0.033 0.024 0.011
0.018
0.013
49.9 0.038 0.022 0.025 0.024
0.027
0.007
51.1 0.032 0.019 0.032 0.024
0.027
0.006
52.4 0.030 0.018 0.020 0.023
0.023
0.005
53.6 0.013 0.008 0.014 0.000
0.009
0.006
54.9 0.025 0.015 0.011 0.026
0.019
0.007
56.1 0.015 0.010 0.023 0.008
0.014
0.007
57.4 0.014 0.006 0.004 0.017
0.010
0.006
58.6 0.007 0.013 0.015 0.010
0.011
0.004
59.9 0.013 0.011 0.007 0.002
0.008
0.005
61.1 0.020 0.001 0.022 0.012
0.014
0.010
62.4 0.008 0.030 0.022 0.011
0.018
0.010
63.6 0.004 0.009 0.007 0.002
0.006
0.003
64.9 0.011 0.019 0.020 0.010
0.015
0.005
66.1 0.000 0.011 0.005 0.001
0.004
0.005
67.4 0.007 0.007 0.006 0.014
0.009
0.004
68.6 0.002 0.005 0.013 0.006
0.007
0.005
69.9 0.000 0.011 0.009 0.002
0.006
0.005
71.1 0.013 0.023 0.018 0.022
0.019
0.005
72.4 0.014 0.023 0.010 0.014
0.015
0.006
73.6 0.033 0.008 0.041 0.019
0.025
0.015
74.9 0.019 0.023 0.022 0.032
0.024
0.006
76.1 0.017 0.008 0.021 0.017
0.016
0.006
77.4 0.006 0.015 0.010 0.013
0.011
0.004
Page A32 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Metaboliccagesgroup4: HF+0.006%PFHS
Time
Feedingboutsize(g)
Average feeding
(hr) cage0103 cage0107 cage0203cage0207 bout size (g)
6.1 0.043 0.050 0.060 0.066
0.055
7.4 0.019 0.023 0.037 0.037
0.029
8.6 0.009 0.023 0.014 0.025
0.018
9.9 0.022 0.014 0.011 0.009
0.014
11.1 0.007 0.007 0.025 0.021
0.015
12.4 0.010 0.024 0.006 0.004
0.011
13.6 0.020 0.000 0.015 0.020
0.014
14.9 0.010 0.006 0.011 0.001
0.007
16.1 0.013 0.005 0.006 0.019
0.011
17.4 0.013 0.012 0.007 0.012
0.011
18.6 0.011 0.027 0.021 0.005
0.016
19.9 0.021 0.040 0.008 0.023
0.023
21.1 0.018 0.013 0.014 0.008
0.013
22.4 0.016 0.029 0.010 0.021
0.019
23.6 0.015 0.009 0.025 0.013
0.016
24.9 0.019 0.001 0.024 0.024
0.017
26.1 0.009 0.019 0.024 0.030
0.021
27.4 0.036 0.063 0.056 0.041
0.049
28.6 0.033 0.030 0.044 0.048
0.039
29.9 0.024 0.020 0.049 0.049
0.036
31.1 0.014 0.012 0.036 0.017
0.020
32.4 0.005 0.009 0.019 0.025
0.015
33.6 0.010 0.017 0.005 0.000
0.008
34.9 0.012 0.000 0.018 0.010
0.010
36.1 0.009 0.012 0.013 0.008
0.011
37.4 0.028 0.032 0.015 0.007
0.021
38.6 0.019 0.040 0.020 0.030
0.027
39.9 0.017 0.000 0.012 0.019
0.012
41.1 0.022 0.044 0.021 0.011
0.025
42.4 0.009 0.015 0.007 0.011
0.011
43.6 0.007 0.016 0.010 0.008
0.010
44.9 0.012 0.017 0.030 0.019
0.020
46.1 0.013 0.035 0.011 0.010
0.017
47.4 0.028 0.007 0.029 0.017
0.020
48.6 0.037 0.026 0.037 0.017
0.029
49.9 0.033 0.015 0.035 0.022
0.026
51.1 0.040 0.027 0.027 0.060
0.039
52.4 0.030 0.039 0.032 0.026
0.032
53.6 0.022 0.025 0.023 0.036
0.027
54.9 0.013 0.004 0.001 0.020
0.010
56.1 0.008 0.008 0.021 0.016
0.013
57.4 0.006 0.002 0.006 0.014
0.007
58.6 0.014 0.007 0.018 0.006
0.011
59.9 0.004 0.005 0.017 0.007
0.008
61.1 0.011 0.008 0.015 0.018
0.013
62.4 0.014 0.012 0.014 0.011
0.013
63.6 0.017 0.002 0.009 0.009
0.009
64.9 0.008 0.015 0.000 0.014
0.009
66.1 0.011 0.009 0.022 0.009
0.013
67.4 0.006 0.000 0.001 0.008
0.004
68.6 0.001 0.010 0.017 0.008
0.009
69.9 0.014 0.010 0.013 0.013
0.013
71.1 0.006 0.014 0.015 0.013
0.012
72.4 0.034 0.013 0.011 0.024
0.021
73.6 0.041 0.008 0.035 0.023
0.027
74.9 0.034 0.015 0.030 0.025
0.026
76.1 0.037 0.016 0.019 0.036
0.027
77.4 0.017 0.014 0.025 0.013
0.017
SD (g) 0.010 0.009 0.008 0.006 0.009 0.009 0.009 0.005 0.007 0.003 0.010 0.013 0.004 0.008 0.007 0.011 0.009 0.013 0.009 0.016 0.011 0.009 0.007 0.007 0.002 0.012 0.010 0.009 0.014 0.003 0.004 0.008 0.012 0.010 0.010 0.009 0.016 0.005 0.006 0.009 0.006 0.005 0.006 0.006 0.004 0.002 0.006 0.007 0.006 0.004 0.007 0.002 0.004 0.011 0.015 0.008 0.011 0.005
Metaboliccagesgroup6 HF+0.003%PFOS
Time
Feedingboutsize(g)
| Average feeding
(hr) cage0104cage0108cage0204cage0208 bout size (g)
6.1 0.040 0.017 0.037 0.035
0.032
7.4 0.026 0.000 0.043 0.025
0.024
8.6 0.020 0.010 0.018 0.018
0.017
9.9 0.024 0.036 0.025 0.018
0.026
11.1 0.022 0.015 0.027 0.013
0.019
12.4 0.030 0.009 0.027 0.014
0.020
13.6 0.027 0.003 0.014 0.014
0.015
14.9 0.008 0.019 0.019 0.008
0.014
16.1 0.010 0.001 0.000 0.018
0.007
17.4 0.026 0.013 0.009 0.002
0.013
18.6 0.014 0.013 0.007 0.012
0.012
19.9 0.011 0.008 0.010 0.013
0.011
21.1 0.015 0.017 0.011 0.013
0.014
22.4 0.019 0.010 0.004 0.014
0.012
23.6 0.024 0.013 0.006 0.015
0.015
24.9 0.018 0.010 0.023 0.033
0.021
26.1 0.013 0.031 0.015 0.033
0.023
27.4 0.050 0.013 0.049 0.024
0.034
28.6 0.020 0.046 0.029 0.033
0.032
29.9 0.027 0.016 0.031 0.011
0.021
31.1 0.013 0.006 0.035 0.024
0.020
32.4 0.014 0.003 0.023 0.011
0.013
33.6 0.010 0.016 0.017 0.012
0.014
34.9 0.011 0.012 0.036 0.019
0.020
36.1 0.011 0.004 0.004 0.002
0.005
37.4 0.039 0.023 0.022 0.023
0.027
38.6 0.029 0.007 0.014 0.022
0.018
39.9 0.024 0.013 0.012 0.026
0.019
41.1 0.023 0.004 0.013 0.014
0.014
42.4 0.018 0.016 0.017 0.002
0.013
43.6 0.013 0.003 0.011 0.012
0.010
44.9 0.020 0.020 0.012 0.013
0.016
46.1 0.013 0.010 0.015 0.015
0.013
47.4 0.066 0.010 0.020 0.018
0.029
48.6 0.026 0.016 0.019 0.012
0.018
49.9 0.031 0.029 0.032 0.019
0.028
51.1 0.027 0.029 0.031 0.027
0.029
52.4 0.021 0.019 0.029 0.023
0.023
53.6 0.012 0.014 0.017 0.014
0.014
54.9 0.014 0.012 0.016 0.026
0.017
56.1 0.019 0.005 0.012 0.005
0.010
57.4 0.015 0.006 0.005 0.011
0.009
58.6 0.015 0.014 0.014 0.001
0.011
59.9 0.014 0.015 0.001 0.006
0.009
61.1 0.023 0.010 0.014 0.013
0.015
62.4 0.012 0.013 0.014 0.010
0.012
63.6 0.016 0.001 0.021 0.008
0.012
64.9 0.015 0.017 0.005 0.009
0.012
66.1 0.010 0.002 0.005 0.008
0.006
67.4 0.012 0.008 0.007 0.001
0.007
68.6 0.011 0.003 0.009 0.000
0.006
69.9 0.012 0.014 0.006 0.007
0.010
71.1 0.009 0.014 0.027 0.003
0.013
72.4 0.022 0.008 0.011 0.020
0.015
73.6 0.028 0.028 0.021 0.087
0.041
74.9 0.027 0.021 0.019 0.015
0.021
76.1 0.020 0.031 0.036 0.030
0.029
77.4 0.007 0.016 0.013 0.016
0.013
SD (g) 0.010 0.018 0.004 0.008 0.006 0.010 0.010 0.006 0.008 0.010 0.003 0.002 0.003 0.006 0.007 0.010 0.010 0.018 0.011 0.009 0.013 0.008 0.003 0.012 0.004 0.008 0.010 0.007 0.008 0.008 0.005 0.004 0.002 0.025 0.006 0.006 0.002 0.004 0.002 0.006 0.007 0.005 0.007 0.007 0.006 0.002 0.009 0.006 0.004 0.005 0.005 0.004 0.010 0.007 0.031 0.005 0.007 0.004
Page A33 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time ( hr)
6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
group 1 vs 3 nn p
4 4 0.886 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.200 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.057 4 4 0.686 4 4 0.029 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.343 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.200 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.460 4 4 0.343 4 4 0.200 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686
gr
n
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
Feeding bout size; p-values Mann Whitney U-test
1 vs 4
p
0 .1 1 4
group 1 vs 6 np
0 .4 8 6
group 3 vs 4 4 n r 0 .0-5 7
group 3 vs 6 np
4 0.200
group 4 vs 6 nn p
0 .0 2 9
0 .8 8 6
0.886 4
0.343 4
0 .6 8 6
1.000
0 .6 8 6
0.686 4
0.686 4
0 .6 8 6
0 .8 8 6
0 .8 8 6
0.200 4
0.114 4
0 .4 8 6
0 .0 5 7
1.000
0.200 4
0.486 4
0 .8 8 6
0 .4 8 6
0 .1 1 4
0.343 4
0.686 4
0 .6 8 6
0 .2 0 0
0 .4 8 6
0.486 4
0.486 4
0.200
0 .8 8 6
0 .0 2 9
0.686 4
0.343 4
1.000
0 .3 4 3
0 .8 8 6
0.486 4
0.486 4
0.200
0 .4 8 6
1.000
1.000 4
0.886 4
0 .6 8 6
0 .8 8 6
0 .4 8 6
0.686 4
0.686 4
0 .8 8 6
0 .8 8 6
0 .4 8 6
0.886 4
0.200 4
0 .3 4 3
0 .2 0 0
0 .6 8 6
0.886 4
0.486 4
0 .3 4 3
0 .8 8 6
0 .6 8 6
0.343 4
0.343 4
0 .6 8 6
0 .2 0 0
0 .6 8 6
0.686 4
0.886 4
0 .8 8 6
0 .8 8 6
0 .3 4 3
0.114 4
0.200 4
0 .4 8 6
1.000
0 .8 8 6
1.000 4
0.686 4
0 .3 4 3
0 .6 8 6
0 .0 2 9
0.114 4
0.114 4
1.000
0 .3 4 3
0 .1 1 4
0.486 4
0.029 4
0 .1 1 4
0 .3 4 3
0.200
0.486 4
0.057 4
0 .1 1 4
0 .3 4 3
0 .6 8 6
0.886 4
1.000 4
0 .8 8 6
0 .8 8 6
0 .4 8 6
1.000 4
0.886 4
0 .8 8 6
0 .8 8 6
1.000
0.057 4
0.886 4
0 .3 4 3
0 .3 4 3
0 .6 8 6
0.114 4
0.686 4
0 .3 4 3
0 .2 0 0
0.200
0.057 4
0.200 4
0 .0 2 9
0 .1 1 4
0 .4 8 6
0.200 4
0.343 4
0 .6 8 6
0 .6 8 6
0 .6 8 6
0.686 4
1.000 4
0 .3 4 3
0 .3 4 3
0 .1 1 4
0.343 4
0.486 4
0 .6 8 6
0 .3 4 3
0.200
0.686 4
0.860 4
0 .6 8 6
0 .4 8 6
0 .3 4 3
0.486 4
0.686 4
0 .3 4 3
0 .3 4 3
0 .1 1 4
0.029 4
0.200 4
0 .3 4 3
0 .8 8 6
0 .6 8 6
1.000 4
0.029 4
0 .0 2 9
0 .8 8 6
0 .3 4 3
0.029 4
0.343 4
0 .0 2 9
0 .8 8 6
1.000
0.686 4
0.886 4
1.000
0 .8 8 6
0 .1 1 4
1.000 4
0.200 4
0 .8 8 6
0 .1 1 4
0 .3 4 3
0.140 4
0.686 4
0 .8 8 6
0 .8 8 6
0 .3 4 3
0.886 4
0.343 4
1.000
0 .6 8 6
0 .0 5 7
0.686 4
0.057 4
0 .8 8 6
0 .0 5 7
0 .0 2 9
0.029 4
0.029 4
0.200
0 .0 2 9
0 .4 8 6
0.486 4
0.200 4
0 .8 8 6
0 .3 4 3
0 .6 8 6
0.200 4
0.886 4
0 .4 8 6
0 .4 8 6
0 .4 8 6
0.486 4
0.486 4
0 .8 8 6
0 .6 8 6
0 .4 8 6
0.686 4
0.886 4
0 .6 8 6
1.000
0.200
0.343 4
0.886 4
0 .8 8 6
1.000
0 .8 8 6
0.486 4
0.686 4
0 .8 8 6
0 .8 8 6
0 .3 4 3
0.486 4
0.886 4
0 .8 8 6
0 .6 8 6
0.200
0.343 4
0.343 4
0 .4 8 6
1.000
0 .8 8 6
1.000 4
0.343 4
0 .3 4 3
0 .4 8 6
0 .0 2 9
0.057 4
0.114 4
0 .4 8 6
0 .1 1 4
0 .1 1 4
0.486 4
0.200 4
1.000
0 .3 4 3
0 .8 8 6
0.343 4
0.686 4
0 .8 8 6
0 .6 8 6
1.000
1.000 4
0.057 4
0 .3 4 3
0 .3 4 3
0 .4 8 6
0.886 4
0.114 4
0 .4 8 6
0 .8 8 6
0 .6 8 6
0.686 4
0.686 4
0 .8 8 6
0 .3 4 3
0 .4 8 6
0.057 4
0.886 4
0 .6 8 6
0 .8 8 6
0 .6 8 6
0.343 4
0.686 4
0 .3 4 3
0 .3 4 3
0 .3 4 3
0.114 4
0.343 4
0 .0 5 7
0 .8 8 6
0.200
0.686 4
0.114 4
0 .3 4 3
0 .3 4 3
p< 0 .0 5
Page A34 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Drink intake measurements
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1: HF control
Accumulated drink intake (ml)
Average acc.
cage 0101 cage 0105 cage 0201 cage 0205 d rin k intake (ml)
0.205
0.065
0.129
0.230
0.16
0.720
0.337
0.591
0.580
0.56
0.946
0.658
0.782
0.846
0.81
1.053
0.791
0.784
1.083
0.93
1.209
0.845
0.805
1.297
1.04
1.284
1.166
0.936
1.334
1.18
1.375
1.292
1.208
1.481
1.34
1.602
1.326
1.325
1.610
1.47
1.716
1.448
1.348
1.624
1.53
1.799
1.479
1.373
1.702
1.59
1.836
1.499
1.382
1.806
1.63
1.898
1.530
1.463
1.826
1.68
1.981
1.530
1.564
1.863
1.73
2.002
1.530
1.576
1.891
1.75
2.049
1.778
1.610
1.976
1.85
2.213
1.870
1.610
2.024
1.93
2.280
2.047
1.796
2.024
2.04
2.465
2.264
1.927
2.098
2.19
2.714
2.349
2.268
2.291
2.41
3.045
2.480
2.638
2.436
2.65
3.250
2.581
2.921
2.691
2.86
3.375
2.669
3.022
2.949
3.00
3.523
2.720
3.227
2.967
3.11
3.640
2.863
3.434
3.036
3.24
3.734
3.104
3.738
3.252
3.46
3.796
3.281
3.818
3.353
3.56
4.053
3.602
4.089
3.434
3.79
4.358
3.893
4.292
3.680
4.06
4.508
4.029
4.460
3.765
4.19
4.524
4.072
4.508
3.832
4.23
4.654
4.114
4.517
3.885
4.29
4.716
4.201
4.683
4.004
4.40
4.784
4.325
4.812
4.170
4.52
4.966
4.510
4.908
4.342
4.68
5.096
4.661
5.072
4.499
4.83
5.140
4.925
5.175
4.538
4.94
5.200
5.018
5.251
4.664
5.03
5.343
5.110
5.469
4.848
5.19
5.465
5.280
5.699
4.922
5.34
5.530
5.423
5.895
5.037
5.47
5.694
5.540
6.008
5.175
5.60
5.892
5.799
6.279
5.348
5.83
6.146
5.984
6.385
5.428
5.99
6.297
6.086
6.578
5.481
6.11
6.399
6.137
6.760
5.557
6.21
6.604
6.372
6.937
5.612
6.38
6.604
6.499
7.038
5.653
6.45
6.695
6.511
7.075
5.773
6.51
6.796
6.511
7.222
5.865
6.60
6.812
6.547
7.270
5.865
6.62
6.812
6.562
7.447
5.900
6.68
6.812
6.800
7.544
6.056
6.80
6.942
6.848
7.694
6.148
6.91
7.192
7.031
7.822
6.297
7.09
7.311
7.293
7.852
6.387
7.21
7.535
7.560
8.022
6.608
7.43
7.639
7.701
8.317
6.845
7.63
7.875
7.752
8.349
7.043
7.75
SD (m l) 0.08 0.16 0.12 0.16 0.25 0.18 0.12 0.16 0.17 0.20 0.22 0.21 0.22 0.23 0.20 0.25 0.20 0.23 0.21 0.28 0.30 0.29 0.34 0.36 0.33 0.28 0.33 0.32 0.36 0.34 0.36 0.35 0.32 0.30 0.30 0.29 0.27 0.27 0.33 0.35 0.35 0.38 0.41 0.47 0.51 0.56 0.58 0.55 0.57 0.59 0.64 0.61 0.63 0.63 0.61 0.59 0.60 0.54
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
Accumulated drink intake (ml)
Average acc.
cage 0102 cage 0106 cage 0202 cage 0206 d rin k intake (ml)
0.125
0.077
0.074
0.092
0.09
0.387
0.392
0.569
0.388
0.43
0.699
0.496
0.967
0.560
0.68
0.883
0.510
1.102
0.676
0.79
0.912
0.691
1.253
0.864
0.93
0.994
0.800
1.449
1.024
1.07
1.176
0.824
1.634
1.276
1.23
1.318
0.834
1.800
1.400
1.34
1.462
0.848
1.850
1.400
1.39
1.565
0.861
2.153
1.450
1.51
1.592
0.872
2.254
1.500
1.55
1.600
0.880
2.311
1.500
1.57
1.659
0.880
2.578
1.548
1.67
1.730
0.923
2.743
1.616
1.75
1.768
1.003
2.804
1.780
1.84
1.837
1.013
3.065
1.842
1.94
1.898
1.024
3.283
1.988
2.05
2.045
1.078
3.512
2.146
2.20
2.210
1.179
3.904
2.280
2.39
2.357
1.194
4.171
2.418
2.53
2.539
1.205
4.361
2.606
2.68
2.710
1.216
4.558
2.764
2.81
2.912
1.232
4.669
2.906
2.93
2.968
1.245
4.869
2.988
3.02
3.101
1.302
4.941
3.026
3.09
3.251
1.360
5.004
3.248
3.22
3.547
1.386
5.263
3.424
3.41
3.787
1.453
5.465
3.520
3.56
3.955
1.557
5.645
3.520
3.67
4.046
1.664
5.702
3.590
3.75
4.059
1.725
5.731
3.632
3.79
4.130
1.776
5.814
3.722
3.86
4.232
1.864
6.048
3.788
3.98
4.293
1.925
6.185
3.874
4.07
4.387
2.098
6.361
4.048
4.22
4.488
2.166
6.577
4.100
4.33
4.763
2.238
6.869
4.252
4.53
5.006
2.384
6.988
4.420
4.70
5.194
2.560
7.207
4.608
4.89
5.344
2.587
7.337
4.804
5.02
5.506
2.672
7.529
5.032
5.18
5.597
2.782
7.729
5.188
5.32
5.693
2.838
7.884
5.352
5.44
5.742
2.869
8.100
5.420
5.53
5.842
2.960
8.217
5.510
5.63
6.122
3.056
8.334
5.646
5.79
6.157
3.131
8.496
5.700
5.87
6.176
3.146
8.674
5.836
5.96
6.198
3.157
8.726
5.900
6.00
6.213
3.168
8.748
5.946
6.02
6.235
3.224
8.748
6.000
6.05
6.250
3.272
8.748
6.000
6.07
6.301
3.341
8.975
6.072
6.17
6.358
3.434
9.104
6.260
6.29
6.584
3.462
9.446
6.362
6.46
6.795
3.486
9.756
6.532
6.64
6.912
3.550
9.992
6.730
6.80
6.998
3.640 10.177 6.890
6.93
SD (m l) 0.02 0.09 0.21 0.26 0.24 0.27 0.33 0.40 0.41 0.53 0.57 0.59 0.70 0.75 0.74 0.85 0.93 1.00 1.13 1.23 1.29 1.37 1.40 1.48 1.49 1.49 1.59 1.65 1.68 1.66 1.65 1.66 1.72 1.75 1.75 1.81 1.90 1.89 1.91 1.95 1.99 2.03 2.07 2.14 2.15 2.17 2.20 2.26 2.28 2.28 2.26 2.24 2.30 2.32 2.44 2.56 2.63 2.67
Page A35 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Tim e (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
M eta bolic ca g e s gro u p 4 : H F + 0 .006 % P F H S
A c c u m u la te d d rin k in take (m l)
Average acc.
c a g e 0 1 0 3 c a g e 0 1 0 7 c a g e 0 2 0 3 c a g e 02 0 7 drink intake (ml)
0.131
0.362
0 .2 1 4
0 .3 0 6
0.25
0.361
0.782
0 .8 8 0
0 .8 4 9
0.72
0 .4 7 9
0 .9 9 6
1.327
1.135
0.98
0 .5 7 4
1.207
1.512
1.254
1.14
0.612
1.207
1.718
1.395
1.23
0 .6 6 9
1.248
1.961
1.518
1.35
0 .7 6 8
1.343
1.995
1.558
1.42
0 .8 1 9
1.370
2 .0 8 5
1.606
1.47
0.982
1.377
2 .1 8 4
1.624
1.54
1.113
1.377
2 .1 8 4
1.694
1.59
1.121
1.550
2 .2 9 7
1.729
1.67
1.159
1.758
2 .3 1 0
1.815
1.76
1.290
1.880
2 .3 2 9
1.971
1.87
1.463
1.938
2 .5 2 0
2 .0 2 4
1.99
1.516
1.955
2 .5 4 5
2 .0 2 4
2.01
1.592
1.955
2 .7 4 7
2.081
2.09
1.615
1.955
2 .9 8 2
2.152
2.18
1.685
2.072
3 .1 3 7
2 .4 2 9
2.33
2 .0 4 8
2 .7 0 8
3 .6 2 0
2 .9 7 4
2.84
2 .3 2 9
2 .9 4 8
4 .0 5 3
3 .5 1 8
3.21
2 .4 5 3
3 .0 7 4
4 .5 7 4
3 .8 4 6
3.49
2.512
3 .1 8 6
4 .8 9 3
4.052
3.66
2 .5 4 6
3 .3 4 9
4 .9 9 8
4.092
3.75
2 .5 9 5
3.451
4 .9 9 8
4 .1 4 5
3.80
2 .6 4 5
3.461
5 .0 9 3
4.312
3.88
2 .6 8 3
3.667
5 .1 8 7
4.312
3.96
2.831
3 .8 5 6
5.271
4 .3 9 6
4.09
2 .9 4 5
3 .9 1 0
5 .4 1 4
4 .5 7 6
4.21
3.027
4 .1 7 5
5 .5 2 7
4 .7 0 8
4.36
3 .1 7 3
4.352
5 .5 6 5
4.717
4.45
3.192
4.352
5 .6 4 9
4.752
4.49
3.211
4 .4 2 3
5 .7 1 2
4 .7 5 9
4.53
3 .2 7 6
4 .4 9 8
5 .9 4 3
4 .8 8 8
4.65
3 .4 1 6
4.651
6 .2 0 3
4 .9 5 0
4.81
3 .5 8 5
4 .7 6 3
6 .4 2 2
4 .9 5 0
4.93
3 .7 5 3
4 .8 5 9
6 .7 6 6
5 .0 2 5
5.10
4.051
5 .0 0 5
7 .0 4 6
5 .3 5 0
5.36
4 .3 6 6
5 .2 6 3
7 .3 8 8
5 .8 7 0
5.72
4 .5 7 9
5.632
7 .6 4 2
6 .3 9 5
6.06
4 .5 7 9
5 .7 9 4
7 .8 5 4
6 .7 4 3
6.24
4 .5 7 9
5 .8 6 8
7 .8 9 2
7 .0 1 8
6.34
4 .5 7 9
5 .9 3 0
8 .1 0 0
7.227
6.46
4 .6 9 9
6.011
8 .2 4 5
7 .4 5 4
6.60
4 .8 0 3
6.042
8 .3 9 2
7 .6 0 8
6.71
4.872
6 .1 0 6
8 .6 1 0
7 .7 2 6
6.83
4 .9 4 0
6 .1 8 8
8 .7 3 6
7 .8 0 6
6.92
4 .9 7 8
6.297
8 .7 3 6
7 .8 5 4
6.97
5 .0 4 3
6.351
8 .7 3 6
7 .9 2 0
7.01
5 .0 8 8
6 .4 5 0
8 .7 6 3
8 .0 0 4
7.08
5.111
6 .4 6 0
8 .9 0 4
8 .0 7 4
7.14
5.111
6 .5 0 4
8 .9 8 8
8 .1 4 0
7.19
5.172
6.542
9 .1 1 4
8 .1 7 5
7.25
5.187
6 .6 6 4
9 .2 8 4
8 .2 2 8
7.34
5 .3 2 4
6 .7 2 5
9 .3 0 3
8.391
7.44
5 .5 9 6
6.732
9 .5 7 4
8.532
7.61
5.917
6 .7 7 3
9.811
8 .7 0 8
7.80
6 .2 3 6
6 .9 4 3
10.019
9.082
8.07
6 .3 3 5
7 .1 3 3
10.185
9.297
8.24
SD (ml) 0.10 0.24 0.36 0.40 0.46 0.54 0.51 0.53 0.50 0.46 0.49 0.47 0.43 0.43 0.42 0.48 0.58 0.62 0.65 0.74 0.92 1.04 1.05 1.02 1.06 1.06 1.02 1.04 1.05 0.99 1.02 1.03 1.10 1.14 1.16 1.25 1.25 1.27 1.29 1.39 1.44 1.54 1.57 1.60 1.67 1.69 1.67 1.64 1.64 1.69 1.73 1.75 1.79 1.77 1.78 1.78 1.78 1.80
Tim e (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 3 9 .9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
M eta bolic ca g e s gro u p 6 H F + 0 .003 % P F O S
A ccu m ulated d rin k intake (m l)
I Average acc.
c a g e 0 1 0 4 c a g e 0 1 0 8 c a g e 0 2 0 4 c a g e 0 2 0 8 drink intake (ml)
0 .1 3 4
0 .2 4 0
0.151
0.202
0.18
0.711
0 .2 8 8
0 .7 2 4
0 .4 7 0
0.55
1.078
0 .3 2 8
1.159
0 .6 1 6
0.80
1.282
0 .5 6 3
1.269
0 .7 1 6
0.96
1.576
0 .8 1 3
1.505
0 .8 4 0
1.18
1.877
0 .8 8 3
1.667
0 .9 7 6
1.35
2 .1 7 9
0 .8 9 6
1.818
1.062
1.49
2 .3 9 4
0.941
1.957
1.100
1.60
2.567
0 .9 9 2
2 .0 1 6
1.216
1.70
2 .6 6 9
1.085
2 .0 1 6
1.280
1.76
2 .8 2 0
1.146
2 .0 1 6
1.288
1.82
2 .9 4 8
1.253
2 .0 1 6
1.360
1.89
2.992
1.264
2 .0 1 6
1.422
1.92
2.992
1.278
2 .0 1 6
1.446
1.93
3.012
1.312
2 .0 1 6
1.560
1.98
3.135
1.312
2 .0 8 8
1.576
2.03
3 .1 9 6
1.338
2 .1 6 0
1.630
2.08
3 .3 4 4
1.506
2 .1 9 8
1.794
2.21
3.611
1.768
2.362
2.022
2.44
3.822
2 .0 9 3
2.907
2 .1 9 8
2.75
4 .0 3 9
2 .1 9 8
3 .3 0 8
2 .3 9 0
2.98
4.097
2 .3 1 7
3 .4 3 8
2 .5 3 4
3.10
4.097
2 .3 9 4
3 .6 2 0
2 .6 6 4
3.19
4.097
2 .5 5 7
3 .9 7 8
2 .8 0 8
3.36
4.097
2 .6 4 2
4 .1 0 4
2 .9 4 0
3.45
4 .1 4 8
2 .7 2 0
4.111
3 .0 0 0
3.49
4 .4 2 3
2 .7 7 4
4 .1 7 6
3 .1 3 4
3.63
4 .6 4 8
2 .8 3 2
4 .3 6 3
3.342
3.80
4 .7 8 4
2 .8 6 4
4.437
3 .4 6 8
3.89
4 .9 4 4
2 .9 0 2
4 .4 6 4
3 .4 8 0
3.95
5 .1 1 7
3 .0 2 4
4 .5 2 9
3 .4 9 4
4.04
5.151
3 .0 2 4
4 .6 9 8
3 .5 0 6
4.09
5 .1 5 6
3 .0 4 6
4 .6 9 8
3 .5 5 0
4.11
5 .2 0 2
3 .1 7 8
4 .6 9 8
3 .6 3 6
4.18
5 .2 6 3
3 .2 0 5
4 .6 9 8
3.732
4.22
5 .5 3 5
3 .3 3 4
4 .8 0 6
3 .8 5 8
4.38
5 .8 7 9
3 .6 2 4
5 .1 2 3
3 .9 7 4
4.65
5 .9 6 4
3 .8 9 8
5.377
4 .1 4 8
4.85
6.137
4 .2 4 2
5 .5 5 5
4 .3 7 8
5.08
6.392
4 .3 4 6
5.767
4 .5 6 0
5.27
6.681
4 .5 3 6
5 .9 9 4
4 .6 7 6
5.47
6.871
4 .6 0 8
6 .1 1 8
4 .7 7 8
5.59
6 .9 8 9
4 .7 0 7
6.311
4 .9 5 8
5.74
7.342
4.891
6 .3 9 0
5 .0 2 0
5.91
7.431
4 .9 1 2
6 .6 0 4
5 .2 1 2
6.04
7.497
5 .0 2 2
6.772
5 .3 4 6
6.16
7 .6 1 6
5 .1 5 2
6 .8 0 4
5 .4 5 2
6.26
7 .6 1 6
5 .1 6 8
6 .8 0 4
5 .5 1 6
6.28
7 .6 1 6
5 .2 1 6
6 .8 0 4
5 .5 4 0
6.29
7 .6 5 9
5 .2 2 2
6 .8 0 4
5 .5 4 0
6.31
7.701
5 .2 3 2
6 .8 0 4
5 .5 4 0
6.32
7.701
5 .2 9 0
6 .8 0 4
5 .5 6 8
6.34
7.701
5 .3 4 7
6 .8 0 4
5 .6 3 0
6.37
7.701
5 .4 0 8
6 .8 0 4
5 .6 7 6
6.40
7 .8 1 3
5 .5 0 4
6 .9 4 4
5 .8 2 4
6.52
8 .0 5 0
5 .6 7 8
7 .1 7 5
5 .9 5 2
6.71
8 .1 0 9
5 .9 8 7
7.547
6 .0 9 8
6.94
8 .1 0 9
6 .2 4 2
7 .6 8 6
6 .3 0 4
7.09
SD (ml) 0.05 0.21 0.39 0.37 0.41 0.50 0.61 0.69 0.73 0.73 0.77 0.78 0.78 0.77 0.75 0.81 0.82 0.81 0.82 0.80 0.85 0.83 0.80 0.79 0.77 0.74 0.80 0.85 0.88 0.93 0.95 1.00 0.98 0.93 0.93 0.98 1.04 0.99 0.92 0.98 1.04 1.09 1.09 1.17 1.18 1.17 1.16 1.14 1.12 1.13 1.15 1.12 1.09 1.06 1.06 1.10 1.06 0.95
Page A36 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
T im e (h r) 6 .1 7 .4 8 .6 9 .9 1 1 .1 1 2 .4 1 3 .6 1 4 .9 1 6 .1 1 7 .4 1 8 .6 1 9 .9 2 1 .1 2 2 .4 2 3 .6 2 4 .9 2 6 .1 2 7 .4 2 8 .6 2 9 .9 3 1 .1 3 2 .4 3 3 .6 3 4 .9 3 6 .1 3 7 .4 3 8 .6 3 9 .9 4 1 .1 4 2 .4 4 3 .6 4 4 .9 4 6 .1 4 7 .4 4 8 .6 4 9 .9 5 1 .1 5 2 .4 5 3 .6 5 4 .9 5 6 .1 5 7 .4 5 8 .6 5 9 .9 6 1 .1 6 2 .4 6 3 .6 6 4 .9 6 6 .1 6 7 .4 6 8 .6 6 9 .9 7 1 .1 7 2 .4 7 3 .6 7 4 .9 7 6 .1 7 7 .4
group 1 v s 3
nn
44
p 0 .3 4 3
44
0 .3 4 3
44 44 44 44
0 .4 8 6
0 .6 8 6 0 .8 8 6 0 .6 8 6
44 44 44 44 44
0 .4 8 6
0 .6 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6
44
44
44 44 44 44 44
0 .8 6 0
0 .8 8 6 1 .0 0 0 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .8 8 6
44
0 .4 8 6
44
0 .3 4 3
44 44 44 44
0 .4 8 6
0 .6 8 6 0 .6 8 6 0 .6 8 6
44
0 .3 4 3
44
0 .3 4 3
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .3 4 3
44
0 .3 4 3
44
0 .3 4 3
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44 44 44 44 44 44 44
0 .4 8 6
0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0 .4 8 6
44
0. 3 4 3
Accumulated drink intake; p-values Mann Whitney U-test
group vsg r o u p 1 v s 4 g r o u p 1 v s 6 g r o u p 3 v s 4 g r o u p 3 v s 6
46
nn p nn 4 4 I 0 .3 4 3 I 4 4 4 4 0 .2 0 0 4 4 4 4 0 .3 4 3 4 4 4 4 0 .3 4 3 4 4 4 4 0 .4 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 44
qqrrmm LL4 4
4 4 0 686 4 4 4 4 0 .4 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 1 .0 0 0 4 4
4 qrm L 4
4 4 0 343 4 4 4 4 0 486 4 4 4 4 0 486 4 4 4 4 0 .4 8 6 4 4 4 4 0 .4 8 6 4 4 4 4 0 .4 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 686 4 4 4 4 0 686 4 4 4 4 0 686 4 4 4 4 0 .6 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 686 4 4 4 4 0 886 4 4
4 qrm L 4
4 4 0 886 4 4 4 4 0 .8 8 6 4 4 4 4 0 .6 8 6 4 4 4 4 0 .4 8 6 4 4 4 4 0 .4 8 6 4 4 4 4 0 486 4 4 4 4 0 486 4 4 4 4 0 .6 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 886 4 4 4 4 0 .8 8 6 4 4 4 4 0 886 4 4 4 4 0 886 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 .8 8 6 4 4 4 4 0 886 4 4 4 4 0 886 4 4 4 I" 4 I 0 . 8 8 6 | 4 | 4
0 .6 8 6 0 .8 8 6 1 .0 0 0 1 .0 0 0 0 .6 8 6 0 .8 8 6 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 0 .8 8 6 0 .8 8 6 0 .8 8 6 0 .6 8 6 0 .8 8 6 0 .8 8 6 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 0 .8 8 6 0 .6 8 6 0 .6 8 6 0 .8 8 6 0 .8 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6
0 .4 8 6
0 .6 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6
0 .8 8 6
0 .8 8 6
0 .8 8 6
0 .8 8 6
0 .8 8 6
0 .8 8 6
0 .8 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .4 8 6
0 .4 8 6
0 .3 4 3
0 .3 4 3
0 .4 8 6
0 .3 4 3
0 .3 4 3
0 .0 2 9 0 .3 4 3 0 .3 4 3 0 .2 0 0 0 .4 8 6 0 .4 8 6 0 .6 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6 0 .6 8 6 0 .4 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .8 8 6 0 .8 8 6 0 .6 8 6 0 .6 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .4 8 6 0 .6 8 6 0 .4 8 6 0 .4 8 6
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
0 .0 2 9 0 .4 8 6 0 .6 8 6 0 .4 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .6 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6 0 .8 8 6 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0 1 .0 0 0
nn
0 .3 4 3
0 .2 0 0
0 .6 8 6
0 .8 8 6
1 .0 0 0
1 .0 0 0
0 .8 8 6
0 .8 8 6
0 .8 8 6
1 .0 0 0
0 .8 8 6
0 .8 8 6
1 .0 0 0
0 .6 8 6
0 .8 8 6
0 .8 8 6
1 .0 0 0
0 .8 8 6
0 .3 4 3
0 .3 4 3
0 .4 8 6
0 .6 8 6
0 .6 8 6
0 .4 8 6
p< 0 .0 5
0 .4 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .4 8 6
0 .4 8 6
0 .4 8 6
0 .4 8 6
0 .2 0 0
0 .2 0 0
0 .4 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .6 8 6
0 .4 8 6
0 .4 8 6
0 .3 4 3
0 .3 4 3
age 3 / of A 1 72
Appendix to TNO-report of 3M-02
Final version
04-12-2006
D4. X activity measurements
Tim e
(hr) 6.1 7.4 8 .6 9 .9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1 : H F control
X activity per interval
A verag e
cage 0101 cage 0105 cage 0201 cage 0205 X activity
100.7
88.8
134.7
99.5
105.9
63.9
227.7
123.3
73.9
122.2
67.5
124.2
57.2
69.0
79.5
76.1
238.3
182.4
62.8
139.9
90.7
71.1
72.9
173.1
102.0
88.6
74.3
128.0
80.4
92.8
69.1
109.8
54.7
35.5
67.3
72.2
28.9
67.7
67.7
59.1
14.3
92.9
70.7
46.6
56.1
112.4
64.9
119.1
63.5
90.0
144.9
81.0
41.1
47.5
78.6
74.2
81.1
92.5
166.7
103.6
44.1
99.0
49.7
29.3
55.5
47.4
71.0
121.8
94.7
83.7
88.3
91.9
108.7
57.4
86.6
424.8
330.7
375.7
615.8
436.8
133.7
276.6
421.4
814.9
411.7
331.4
346.2
385.6
228.3
322.9
121.9
221.1
257.7
488.8
272.4
143.4
139.3
121.1
158.0
140.5
133.1
229.4
213.0
217.7
198.3
74.2
181.4
129.7
250.7
159.0
54.6
182.5
199.9
94.7
132.9
172.7
166.9
158.2
119.2
154.3
84.7
128.2
53.6
109.3
94.0
101.7
122.5
136.4
118.5
119.8
53.4
25.2
67.8
54.2
50.2
33.9
49.9
56.4
55.9
49.0
84.9
47.7
44.7
23.0
50.1
28.9
79.6
74.6
119.0
75.5
64.8
34.9
59.5
96.2
63.9
74.9
64.0
65.7
38.0
60.7
69.8
101.4
87.1
72.6
82.7
90.4
52.7
46.1
71.2
65.1
373.3
279.0
284.9
313.7
312.7
337.7
147.0
370.8
371.0
306.6
361.6
270.6
276.3
229.2
284.4
161.5
365.6
199.2
227.9
238.6
164.7
143.5
156.8
202.0
166.8
119.6
206.4
153.3
185.1
166.1
76.2
117.9
136.6
57.6
97.1
82.3
180.9
143.9
106.5
128.4
96.9
97.8
154.2
234.6
145.9
173.9
144.2
96.5
86.3
125.2
90.2
68.3
85.5
90.4
83.6
71.4
80.6
87.9
29.3
67.3
49.0
185.5
93.7
91.4
104.9
94.4
67.4
115.8
76.0
88.4
36.2
14.6
23.2
57.4
32.9
91.6
118.8
97.4
31.4
84.8
42.5
77.5
37.2
106.7
66.0
50.0
112.7
53.7
40.3
64.2
94.0
43.9
83.9
120.3
85.5
186.7
126.5
215.6
235.7
191.1
380.3
349.8
374.6
551.9
414.2
411.4
223.4
274.0
372.4
320.3
246.9
230.0
248.0
185.3
227.6
156.4
360.5
270.5
260.9
262.1
SD
19.9 75.0 30.3 84.7 48.3 24.2 31.5 20.3 33.7 29.9 4 7 .5 42.7 30.2 31.9 21.4 125.4 293.4 67.1 155.3 15.2 44.0 75.2 69.6 24.1 32.3 14.3 17.9 10.5 25.7 36.9 25.2 15.8 14.6 19.9 43.1 107.6 55.6 89.0 25.1 37.9 36.5 43.2 64.9 41.1 10.4 26.2 57.5 21.5 18.6 37.5 32.5 32.8 31.7 4 7 .5 92.8 86.7 29.3 83.5
Tim e
(hr) 6.1 7.4 8 .6 9 .9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
M etabolic cages group 3: H F + 0 .0 3 % PFBS
X activity per interval
Average
cage 0102 cage 0 106 cage 0202 cage 0206 X activity
92.2
254.5
88.7
185.0
155.1
9 2 .7
238.4
92.6
375.7
199.9
79.1
99.1
110.4
111.4
100.0
131.6
82.0
45.9
221.7
120.3
134.8
124.3
259.8
60.1
144.8
5 4 .7
138.5
174.2
39.8
101.8
134.4
53.3
68.6
182.7
109.8
5 9 .0
82.5
103.6
47.8
73.2
122.4
105.6
146.3
18.4
98.2
8 4 .7
29.6
245.5
93.8
113.4
158.6
172.4
76.4
41.6
112.3
2 1 .0
39.6
50.1
14.5
31.3
9 3 .0
113.8
169.7
118.6
123.8
111.5
160.0
89.9
126.0
121.9
158.6
55.4
169.7
72.4
114.0
454.8
579.9
431.8
455.2
480.4
432.8
621.4
618.1
933.4
651.4
277.4
351.9
422.6
616.8
417.2
325.6
48.8
191.6
130.5
174.1
262.1
373.9
151.6
282.2
267.5
9 5 .4
52.6
238.0
216.4
150.6
127.8
215.5
260.5
148.1
188.0
9 9 .6
135.3
127.1
112.9
118.7
199.2
141.3
217.7
267.4
206.4
135.8
276.8
172.6
73.8
164.8
109.4
98.3
200.9
103.7
128.1
5 8 .6
61.8
132.5
28.6
70.4
6 1 .9
88.5
155.1
26.9
83.1
6 0 .3
164.0
35.5
11.7
67.9
4 7 .7
32.8
87.4
223.3
97.8
69.2
57.8
71.1
68.3
66.6
131.2
97.6
173.2
60.2
115.6
8 8 .0
78.5
143.7
50.2
90.1
120.8
63.9
104.5
30.4
79.9
361.3
203.4
323.6
485.2
343.4
527.6
382.4
442.4
1 1 9 4 .3
636.7
308.7
470.4
298.9
609.6
421.9
201.9
177.0
84.2
327.0
197.5
332.9
80.5
218.2
274.2
226.5
117.4
102.8
219.6
242.1
170.5
136.8
300.6
165.3
167.6
192.6
121.2
113.3
113.2
309.0
164.2
177.5
352.3
305.4
374.6
302.5
236.1
139.3
186.5
212.9
193.7
70.6
171.6
92.8
28.1
90.8
102.9
131.2
71.9
161.7
116.9
72.1
72.4
43.7
9.3
49.4
6 2 .0
63.4
125.6
266.9
129.5
130.8
49.7
53.5
23.2
64.3
75.2
27.2
84.9
221.5
102.2
8 4 .0
116.4
51.9
45.1
74.4
63.1
82.3
73.4
35.7
63.6
129.7
62.8
151.3
92.7
109.1
201.6
143.3
125.5
219.4
172.5
429.8
396.0
387.1
405.0
404.5
278.5
493.7
280.0
701.6
438.5
270.4
175.4
217.9
407.2
267.7
256.5
298.8
295.6
603.1
363.5
SD
79.9 135.9 15.0 76.2 83.5 64.9 60.0 24.9 55.7 92.5 63.4 16.4 32.6 29.4 58.5 67.2 207.6 145.7 116.7 91.3 90.6 61.2 15.8 52.1 85.1 48.8 44.0 54.2 67.1 86.8
6.0 48.1 39.2 40.8 116.1 376.5 147.8 100.1 108.0 70.6 73.4 96.6 88.2 41.5 60.2 38.4 29.9 96.3 46.3 83.4 32.8 20.2 39.2 45.1 18.4 202.5 100.8 160.9
Page A38 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 4: HF + 0.006 % PFHS
X activity per interval
Average
cage 0103 cage 0107 cage 0203 cage 0207 X activity
75.4 118.1 92.7 258.6
136.2
91.1
61.6
100.7
151.8
101.3
32.3 128.9 85.4 124.6
92.8
88.7 58.6 66.1 53.7
66.8
56.0 47.9 89.9 144.3
84.5
79.3 139.0 66.9 185.7
117.7
103.8
31.0
43.1
57.7
58.9
51.9 53.5 153.0 95.1
88.4
65.2
199.5
53.3
90.9
102.2
83.7
172.4
74.7
34.6
91.4
28.4 46.4 42.0 124.4
60.3
59.3 94.9 103.2 76.9
83.6
108.0 72.6 51.6 57.3
72.4
59.8
39.3
122.1
273.1
123.6
71.2 76.4 74.7 95.4
79.4
484.3
589.1
168.2
410.3
413.0
821.1
605.3
368.9
719.7
628.8
418.6
371.6
219.7
566.0
394.0
282.1
99.3
260.1
379.5
255.3
175.2
215.2
227.2
177.6
198.8
203.6
190.6
144.7
107.9
161.7
92.2 84.7 83.6 169.4
107.5
146.6
178.0
157.2
185.6
166.9
85.3
14.1
101.8
125.7
81.7
173.4
94.0
82.6 336.9
171.7
141.5
111.9
103.3
374.0
182.7
97.6 65.8 82.8 63.6
77.5
46.4 28.1 47.1 140.5
65.5
54.7 69.4 73.6 65.1
65.7
47.0 47.6 44.3 73.3
53.1
79.7 55.9 49.8 76.8
65.6
33.1 77.6 137.3 47.2
73.8
141.3
169.8
67.1
187.9
141.5
148.8
21.7
77.9
55.3
75.9
211.0
139.5
118.3
271.5
185.1
555.2
277.4
176.1
489.8
374.6
227.0
219.5
308.1
548.7
325.8
192.2
120.1
214.5
482.5
252.3
145.5
229.0
129.1
229.2
183.2
159.5
85.7
211.4
146.7
150.8
114.2
96.2
119.5
189.7
129.9
64.4 84.0 123.6 69.4
85.4
116.3
161.9
141.4
211.7
157.8
59.8 24.8 79.1 127.3
72.8
98.7 39.5 90.8 97.0
81.5
97.6 87.9 60.4 116.3
90.6
97.9 39.2 108.8 93.5
84.9
87.3
120.3
55.8
55.3
79.7
69.4
44.1
120.1
211.5
111.3
63.4 28.1 69.2 83.9
61.2
34.3 79.2 92.1 126.5
83.0
77.4
110.7
103.2
151.6
110.7
54.9 103.1 77.5 66.3
75.5
121.3
183.4
84.3
76.1
116.3
262.2
613.3
156.4
233.3
316.3
393.8
463.2
326.7
686.7
467.6
152.2
146.0
423.4
234.1
238.9
160.4
290.0
246.2
300.4
249.3
SD
83.5 37.6 44.8 15.5 43.8 55.2 31.9 47.5 66.7 58.1 43.4 19.6 25.4 105.7 10.9 178.9 194.4 142.7 116.2 26.3 43.9 41.5 18.1 48.0 117.3 128.6 15.9 50.8 8.1 13.6 14.9 46.2 53.2 53.8 69.9 177.7 153.9 158.7 53.4 51.6 41.1 26.8 40.5 42.8 28.2 23.3 31.1 30.9 73.9 23.7 38.1 30.8 20.6 48.9 203.0 156.3 129.4 63.7
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 6 HF + 0.003 % PFOS
X activity per interval
| Average
cage 0104 cage 0108 cage 0204 cage 0208 X activity
152.1
223.2
114.3
130.4
155.0
136.3
217.5
107.7
211.5
168.3
37.5 132.4 78.6
35.1
70.9
120.1
164.6
61.9
77.3
106.0
165.9
124.0
96.1
64.6
112.7
64.9 56.9 76.3 42.0
60.0
99.3 51.5 54.9 136.8
85.6
66.3
168.5
118.2
51.8
101.2
35.2 74.0 67.3 72.4
62.2
29.9 94.3 55.5 23.5
50.8
75.5
152.4
123.9
48.4
100.1
94.7 30.6 41.5 67.2
58.5
61.8 90.8 77.4 96.6
81.7
41.2
176.7
52.9
68.4
84.8
154.0
98.7
119.9
115.8
122.1
248.5
353.9
134.8
128.7
216.5
432.4
353.1
703.1
283.1
442.9
225.6
313.2
437.8
367.4
336.0
250.2
403.1
203.5
248.2
276.3
130.8
117.3
152.3
164.3
141.2
194.5
243.2
125.1
138.7
175.4
136.1
54.8
78.9 156.4
106.6
138.1
137.0
183.6
133.4
148.0
250.1
184.4
140.2
133.4
177.0
164.8 39.0 140.4 63.1
101.8
111.8
88.9
133.9
108.8
110.9
81.0 99.0 41.5 98.2
79.9
64.7 12.4 52.8 123.7
63.4
29.8 48.0 55.2 50.8
46.0
36.2
141.9
24.8
28.7
57.9
73.6
35.9
147.9
112.9
92.6
75.9 45.5 63.5 32.1
54.3
42.3 63.0 57.3 78.0
60.2
38.4 84.7 46.8 106.0
69.0
140.0
152.0
217.4
78.4
147.0
405.1
292.8 232.2
226.6
289.2
131.5
332.3
228.9
252.9
236.4
212.6
410.7
208.7
235.1
266.8
114.7
310.1
107.5 227.4
189.9
136.0
161.8
93.3
83.1
118.6
198.1
73.7
158.3
108.8
134.7
89.1
134.6
91.5
166.0
120.3
136.5
126.4
110.9
175.2
137.3
180.5
105.8
129.4
141.3
139.3
85.9 71.5 90.4 116.7
91.1
56.8 151.5 70.7 107.5
96.6
74.8 40.4 70.0 67.5
63.2
42.2 130.3 32.1
72.8
69.4
26.7 24.3 85.4 91.4
57.0
74.5 80.0 53.0 61.2
67.2
59.1 27.3 68.7 34.4
47.4
57.3 101.8 40.5 102.7
75.6
55.0 80.3 57.9 55.6
62.2
106.5 43.9 112.9 98.0
90.3
193.8
308.6
110.3
109.8
180.6
256.2
142.5
208.6
290.9
224.6
131.1
305.9
223.4
367.3
256.9
203.7
407.8
178.4
337.2
281.8
SD
48.0 54.7 45.6 46.2 43.0 14.4 40.5 53.2 18.2 32.1 46.8 28.6 15.5 62.3 23.2 106.9 183.9 89.5 87.3 21.1 54.3 47.6 23.8 53.7 60.3 18.4 26.9 46.0 11.2 56.2 48.5 19.3 14.8 31.9 57.0 82.9 82.7 96.7 97.1 36.8 54.7 36.9 27.4 31.2 18.9 42.4 15.5 44.2 36.4 12.3 19.7 31.6 12.1 31.5 94.0 64.3 102.5 109.1
Page A39 of A172
Appendix to TNO-report of 3M-02_____________ Final version
04-12-2006
Time ( hr)
6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p
4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.486 4 4 0.686 4 4 0.200 4 4 1.000 4 4 0.486 4 4 0.029 4 4 0.057 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.486 4 4 0.057 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.200 4 4 0.200 4 4 0.686 4 4 0.486 4 4 0.343 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.684 4 4 0.686 4 4 0.029 4 4 0.114 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.029 4 4 0.686 4 4 0.057 4 4 0.114 4 4 0.886 4 4 0.114 4 4 0.114 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.486
X activity; p-values Mann Whitney U-test
group 1 vs 4 group 1 vs 6 | group 3 vs 4 group 3 vs 6
n n p n [Q p n
4 4 | 1.000 I 4 4 0.114 4
1.000 4
0.886
4 4 0.886 4 4 0.486 4
0.343 4
1.000
4 4 0.486 4 4 0.886 4
0.886 4
0.343
4 4 0.200 4 4 0.686 4
0.486 4
0.886
4 4 0.486 4 4 0.886 4
0.343 4
0.686
4 4 0.886 4 4 0.057 4
0.486 4
0.886
4 4 0.686 4 4 0.686 4
0.200 4
0.686
4 4 0.686 4 4 0.686 4
0.886 4
0.486
4 4 0.486 4 4 0.886 4
0.886 4
0.343
4 4 1.000 4 4 0.114 4
4 4 0.48m 4 4 0.486 4 4 4 0.88m 4 4 0.200 4
0.686 4 0.343 4 0.029 4
0.486 0.686 0.200
4 4 0.20m 4 4 0.343 4
0.057 4
0.057
4 4 0.88m 4 4 0.686 4 4 4 0.68m 4 4 0.057 4
0.686 4 0.886 4
0.343 1.000
4 4 1.00m 4 4 0.057 4
0.886 4
0.029
4 4 0.34m 4 4 0.686 4 4 4 0.48m 4 4 1.000 4
0.886 4 0.886 4
0.200 0.686
4 4 0.88m 4 4 1.000 4
0.486 4
0.200
4 4 I 2 9 4 4 1.000 4 4 4 0.20m 4 4 0.686 4
0.343 4 1.000 4
0.114 0.686
4 4 0.48m 4 4 0.486 4 4 4 0.88m 4 4 0.886 4
0.114 4 0.029 4
0.200 0.057
4 4 0.05m 4 4 0.686 4
0.029 4
0.343
4 4 0.486 4 4 0.886 4
0.886 4
0.343
4 4 0.68m 4 4 0.486 4
0.486 4
1.000
4 4 0.114 4 4 0.200 4
4 4 0.68m 4 4 0.686 4
0.343 4 0.686 4
0.686 0.686
4 4 0.34m 4 4 0.686 4
0.486 4
0.886
4 4 0.34m 4 4 0.486 4
0.486 4
0.343
4 4 1.00m 4 4 0.343 4
1.000 4
0.343
4 4 0.88m 4 4 0.686 4 4 4 0.34m 4 4 0.114 4
0.343 4 0.343 4
0.114 0.200
4 4 0.886 4 4 1.000 4
0.886 4
0.886
4 4 10 0 2 9 4 4 0.029 4
4 4 0.68m 4 4 0.886 4
0.114 4 0.486 4
0.057 0.057
4 4 0.88m 4 4 0.343 4 4 4 0.88m 4 4 0.486 4
0.343 4 0.686 4
0.114 0.200
4 4 0.886 4 4 1.000 4
0.686 4
0.886
4 4 0.88m 4 4 0.200 4
0.686 4
0.343
4 4 0.48m 4 4 0.486 4
0.200 4
0.343
4 4 0.20m 4 4 0.886 4
0.200 4
0.686
4 4 0.68m 4 4 0.886 4
0.057 4
0.029
4 4 0.114 4 4 0.686 4
4 4 0.34m 4 4 0.486 4
0.029 4 0.886 4
0.114 0.886
4 4 0.20m 4 4 0.686 4
0.343 4
0.486
4 4 1.00m 4 4 0.200 4
0.200 4
0.886
4 4 | 0.686 | 4 4 0.486 4
0.343 4
0.486
4 4 0.05m 4 4 0.343 4
0.486 4
1.000
4 4 0.20m 4 4 0.343 4
0.486 4
0.486
4 4 0.68m 4 4 0.343 4
0.886 4
0.343
4 4 0.20m 4 4 0.686 4
0.057 4
0.686
4 4 | 0.68m 4 4 0.343 4
0.343 4
0.057
4 4 0.057 4 4 0.029 4
0.114 4
0.029
4 4 0.34m 4 4 0.029 4
0.343 4
0.029
4 4 0.20m 4 4 0.200 4
4 4 0.68m 4 4 0.886 4
m4 4 \ 1.00 4 4 0.886 4
0.886 4 0.686 4 0.343 4
0.114 1.000 0.686
group 4 vs 6
0.486 0.114 0.886 0.200 0.343 0.057 0.486 0.886 0.686 0.343 0.200 0.200 0.486 0.886 0.029 0.114 0.200 0.686 0.886 0.029 0.886 0.686 0.200 0.029 0.343 0.343 0.686 0.886 0.057 0.343 0.686 0.486 0.057 1.000 0.886 0.686 0.886 0.686 0.686 0.486 1.000 0.114 0.486 0.057 0.886 1.000 0.343 0.686 0.343 1.000 0.200 0.114 0.686 0.686 0.343 0.029 1.000 0.686
I
I p<005 I
Page A40 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
D5. O2 consumption,CO2 production and respiratory exchange ratio measurements
O2 consumption
T im e
(h r) 6.1 7 .4 8.6 9.9 11.1 1 2 .4 13.6 14.9 16.1 1 7 .4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M e ta b o lic c a g e s g ro u p 1 : H F c o n t rol
V O 2 (m l/h/kg m ouse)
Average VO2
cage 0101 cage 0105 cage 0201 cage 0205
3055
3609
3572
3183
2909
3769
3278
2987
2786
3499
3070
3028
3051
3540
3607
2913
2741
3200
2943
3204
2814
2966
3194
2903
2636
3102
2744
2661
2685
2756
2766
2705
2399
2814
2770
2676
2811
2699
2874
2756
3115
2850
2610
2730
2766
2917
2939
3098
2602
2990
2628
2542
2743
2708
2828
2892
3001
2983
2979
2785
3803
3693
3803
3939
2959
3356
3539
3857
3391
3518
3497
3027
2921
3300
3385
3544
2968
3266
3115
2992
2981
3263
3316
3042
2763
3180
3307
3199
2742
3182
3401
2921
3160
3090
3203
2842
2844
3097
2702
2680
2831
2938
3274
2716
2671
2564
2826
2528
2400
2696
2776
2658
2690
2672
2630
2450
2344
2796
2790
2828
2542
2523
2642
2639
2549
2679
2872
2558
2757
2884
3000
2753
2937
2860
2704
2758
3585
3535
3750
3397
3458
3277
3756
3348
3320
3475
3528
3083
2985
3469
3327
3112
2933
3202
3213
3143
3066
3289
3231
3090
2657
3247
3191
2815
2818
3230
3258
2922
2822
2877
3274
3079
2899
3156
3119
2652
2683
2820
2794
2648
2389
2882
2765
2368
2451
2707
2939
2554
2727
2568
2982
2495
2399
2276
2392
2563
2588
2697
2816
2448
2392
2652
2564
2667
2532
2656
2612
2506
2612
2511
2816
2723
3115
3156
3363
3306
3315
3487
3910
3443
3280
3320
3488
3288
2917
3251
3373
2915
2855
3337
3389
3106
(ml/h/kg mouse) 3355 3236 3096 3278 3022 2969 2786 2728 2665 2785 2826 2930 2691 2793 2937 3809 3428 3358 3287 3085 3150 3112 3062 3074 2831 2940 2647 2633 2611 2689 2587 2665 2849 2815 3566 3460 3352 3223 3123 3169 2977 3057 3013 2957 2736 2601 2663 2693 2407 2637 2569 2576 2666 3235 3539 3344 3114 3172
SD
278 389 296 347 224 162 216 39 186 75 216 136 203 83 102 101 375 228 264 136 164 239 290 162 192 240 134 163 110 231 63 151 118 104 146 211 199 216 130 108 287 220 206 233 84 262 212 216 118 157 126 70 132 119 258 98 234 244
T im e
(h r) 6.1 7.4 8.6 9.9 11.1 1 2 .4 13.6 14.9 16.1 1 7 .4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M e ta bolic ca g e s g ro u p 3: H F + 0 .0 3 % P F B S
V O 2 (m l/h/kg m ouse)
Average VO2
cage 0102 cage 0106 cage 0202 cage 0206
3472
3369
3160
3514
3395
3409
3368
3614
3050
3203
3449
3293
3172
2947
3117
3489
3251
3125
3816
3179
2926
3060
3395
2899
3037
2695
3096
3070
2788
2851
3097
2552
2871
2875
3325
2576
2799
2652
3518
2756
3056
3007
3074
2662
2601
2709
3117
2514
2805
2795
3413
2879
2750
2921
3103
2867
3171
2587
3536
2788
3833
3748
4154
3592
3636
3978
4043
3820
3312
3246
3549
3655
3416
2692
3359
3071
3230
3470
3388
3377
2869
2582
3516
3131
2856
2876
3552
3029
2994
2690
3323
2895
3079
2731
3477
3112
2968
3042
3324
2751
3173
2525
3366
2900
2704
2513
3166
2538
2718
2533
3179
2470
2628
2616
3017
2540
2607
2201
3282
3134
2730
2354
3253
2736
2797
2485
3350
2734
2698
2627
3198
2680
3046
2525
3342
2752
3577
3124
3851
3637
3845
3499
3852
3908
3451
3441
3668
3515
3181
2990
3312
3294
3346
2800
3445
3165
3086
2879
3554
3289
3090
3022
3415
3186
2863
2881
3403
3262
3087
3127
3591
3313
3056
2850
3467
3039
2671
3145
3078
2635
2444
2789
2892
2715
2399
2700
2935
2380
2342
2391
3212
2899
2545
2295
2815
2392
2404
2324
3006
2846
2463
2660
2803
2507
2455
2428
2845
2482
2691
2326
3266
2798
3248
2864
3489
3160
3502
3251
4078
3573
3217
3329
3610
3467
3102
2931
3421
3432
3005
2951
3571
3348
(ml/h/kg mouse) 3379 3446 3249 3181 3343 3070 2975 2822 2912 2931 2950 2735 2973 2910 3020 3832 3869 3441 3135 3366 3025 3078 2975 3100 3022 2991 2730 2725 2700 2806 2768 2842 2801 2916 3547 3776 3519 3194 3189 3202 3178 3102 3279 3103 2882 2710 2603 2711 2512 2645 2608 2553 2770 3190 3601 3406 3222 3219
SD
158 113 167 226 320 228 188 224 309 396 194 267 296 147 420 237 181 193 332 100 397 325 264 305 237 364 302 320 215 496 369 365 267 355 306 187 105 148 284 289 172 272 230 260 266 191 265 418 227 332 155 196 387 258 346 170 247 293
Page A41 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
T im e
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M eta bolic ca g e s gro u p 4: H F + 0 .006 % P F H S
V O 2 (m l/h/kg m ouse)
Average VO2
cage 0103 cage 0107 cage 0203 cage 0207
3575
3707
3794
3663
3438
3450
3743
3572
3276
3564
3720
3214
3298
3287
3705
3019
3219
3026
3779
3239
3263
3409
3672
3345
3253
2782
3223
2912
3148
2910
3420
2872
3131
3357
3169
2875
3100
3284
3191
2771
3016
2897
2977
2999
3076
3165
3223
2870
3085
2998
2948
2834
3053
2966
3152
3341
3230
3150
3288
3059
4037
4423
3777
3640
4262
4267
4205
4105
3794
3878
3828
3747
3514
3369
3957
3750
3148
3529
3640
3236
3395
3172
3650
3219
3149
2853
3984
3250
3169
3211
3921
3297
2970
2429
3604
3069
3160
2889
3350
3309
3016
3030
3543
3283
2941
2788
3214
2655
2850
2683
3085
2885
2941
2931
3210
2530
2907
2730
2981
2725
2942
2681
2965
2754
2796
2997
3440
2692
3066
3271
3046
3180
3205
2865
3259
2689
3687
3511
3496
3092
4214
3964
4094
3677
3775
3687
4344
3700
3674
3386
3911
3750
3475
3563
3848
3319
3342
2927
3707
3329
3292
3075
3560
3360
3037
2999
3737
3049
3252
3141
3706
3142
2949
2581
3450
2942
2998
2658
3240
2765
3028
2847
3009
2797
2891
2517
3312
2674
2898
2895
3049
2635
2599
2387
3169
3032
2763
2486
2988
2671
2667
2703
3097
2657
2847
2910
3004
2767
2862
2964
3025
2614
3237
3292
3296
2919
3789
4002
3796
3425
3950
3705
4281
3810
3367
2971
4117
3470
3409
3211
3938
3360
(ml/h/kg mouse) 3685 3551 3443 3327 3316 3422 3043 3088 3133 3086 2972 3084 2966 3128 3182 3969 4210 3812 3648 3388 3359 3309 3399 3018 3177 3218 2899 2876 2903 2836 2835 2981 3141 3005 3446 3987 3876 3680 3551 3326 3322 3206 3310 2980 2915 2920 2848 2869 2797 2727 2781 2882 2866 3186 3753 3937 3481 3480
SD
91 141 240 283 323 177 233 253 198 224 53 155 105 161 99 345 75 55 260 234 216 480 352 481 209 249 240 165 280 128 140 331 105 273 252 230 314 220 222 319 200 355 269 357 259 116 345 172 366 209 211 100 181 180 240 251 475 317
Tim e
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M eta bolic cag e s gro u p 6 : H F + 0.003 % P F O S
V O 2 (m l/h/kg m ouse)
Average VO2
cage 0104 cage 0108 cage 0204 cage 0208
3936
4321
3970
3628
3779
3980
3795
3648
3334
3807
3674
3379
3695
3783
3579
3441
3866
3646
3646
3141
3633
3225
3349
3124
3766
3107
3222
3167
3592
3471
3249
3007
3335
2903
3089
3039
3212
3171
2998
2777
3726
3504
3191
2894
3676
2963
2911
3007
3305
3099
2949
3046
3297
3319
2980
3014
3731
3204
3078
3199
4099
4024
3349
3392
4351
3972
4050
3767
3752
3870
4131
3917
3941
4064
3600
3773
3747
3497
3405
3448
3948
3675
3488
3364
3818
3178
3407
3492
3918
3425
3579
3323
3886
3428
3396
3234
3850
3073
3304
3074
3591
3040
3332
3159
3316
3011
2944
3124
3267
2725
2955
3142
3116
2830
2972
3019
3148
3212
2800
2929
3309
2822
3150
3182
3392
2842
2934
2916
3310
2932
3103
3085
3301
2919
2962
3075
3857
3307
3541
3244
4409
3774
3676
3620
3834
3995
3818
3874
4037
3881
3610
3592
3747
3731
3514
3608
3907
3451
3525
3338
3965
3167
3620
3404
3733
3412
3604
3462
3792
3344
3376
3424
3870
3327
3465
3392
3598
3153
3213
3205
3697
3167
3007
3175
3151
2771
3077
2885
3203
2934
2705
3044
2953
2527
2901
2845
3241
2925
2873
2718
3204
2665
2843
2776
3161
2950
2659
3014
3089
2860
2748
2780
3570
2917
2989
3115
3761
3686
3194
3269
3836
3329
3493
3677
3648
3583
3465
3833
3736
3613
3363
3688
(ml/h/kg mouse) 3963 3800 3549 3624 3575 3333 3315 3330 3091 3040 3329 3139 3100 3152 3303 3716 4035 3918 3845 3524 3619 3474 3561 3486 3325 3280 3099 3022 2984 3022 3116 3021 3108 3064 3487 3870 3880 3780 3650 3555 3539 3553 3484 3513 3292 3262 2971 2972 2806 2939 2872 2946 2869 3148 3477 3584 3632 3600
SD
283 136 229 148 307 220 304 258 180 198 364 360 150 180 291 400 242 158 202 153 254 265 260 280 366 239 163 236 119 191 208 250 155 171 278 365 80 217 110 247 339 145 208 245 205 301 174 209 192 220 233 211 154 293 287 220 154 166
Page A42 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time ( hr)
6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
group 1 vs 3 nn p
4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.200 4 4 0.886 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.200 4 4 0.886 4 4 1.000 4 4 0.114 4 4 0.686 4 4 0.686 4 4 0.057 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.057 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.334 4 4 0.886
gr
n
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
VO2 , p-values Mann Whitney U-test
1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6
0.057 0.343 0.114 0.886 0.114 0.029 0.114 0.029 0.029 0.114 0.343 0.343 0.114 0.029 0.029 0.886 0.029 0.029 0.200 0.114 0.343 0.886 0.343 0.686 0.057 0.114 0.200 0.114 0.200 0.686 0.029 0.114 0.029 0.486 0.486 0.057 0.029 0.057 0.029 0.343 0.114 0.686 0.200 1.000 0.486 0.114 0.686 0.343 0.200 0.686 0.057 0.029 0.114 0.686 0.486 0.029 0.200 0.200
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
P
029 057 114 200 114 057 029 029 029 114 057 486 057 029 029 000 057 029 029 029 029 200 057 029 114 114 029 057 029 057 029 057 057 057 686 114 029 029 029 029 114 029 029 029 029 029 114 200 057 057 057 029 114 343 686 057 029 057
0 .0 2 9 0 .2 0 0 0 .3 4 3 0 .4 8 6 0 .8 8 6 0 .1 1 4 0 .6 8 6 0 .1 1 4 0 .2 0 0 0 .4 8 6 0 .4 8 6 0 .1 1 4 0 .4 8 6 0 .1 1 4 0 .6 8 6 0 .6 8 6 0 .0 2 9 0 .0 2 9 0 .0 5 7 0 .8 8 6 0 .2 0 0 0 .6 8 6 0 .2 0 0 0 .6 8 6 0 .4 8 6 0 .4 8 6 0 .3 4 3 0 .4 8 6 0 .6 8 6 1.000 0 .6 8 6 0 .6 8 6 0 .2 0 0 0 .8 8 6 0 .4 8 6 0 .2 0 0 0 .0 2 9 0 .0 2 9 0 .1 1 4 0 .4 8 6 0 .4 8 6 0 .6 8 6 0 .6 8 6 0 .4 8 6 0 .8 8 6 0 .1 4 0 0 .4 8 6 0 .8 8 6 0 .3 4 3 0 .8 8 6 0 .3 4 3 0 .0 5 7 0 .6 8 6 0 .8 8 6 0 .6 8 6 0 .0 2 9 0 .4 8 6 0 .3 4 3
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
p
0.029 0.029 0.114 0.057 0.486 0.200 0.029 0.057 0.200 0.486 0.200 0.200 0.343 0.114 0.343 0.686 0.486 0.029 0.029 0.114 0.114 0.200 0.029 0.200 0.200 0.486 0.200 0.200 0.114 0.686 0.200 0.200 0.200 0.686 0.886 0.886 0.029 0.029 0.029 0.200 0.200 0.029 0.200 0.200 0.029 0.029 0.114 0.486 0.114 0.343 0.114 0.057 0.686 0.886 0.886 0.200 0.029 0.057
group 4 vs 6 nn p
0.200 0.057 0.486 0.200 0.486 0.486 0.686 0.200 0.686 0.886 0.343 0.886 0.200 1.000 0.886 0.486 0.343 0.343 0.343 0.686 0.200 0.486 0.343 0.200 1.000 0.486 0.200 0.343 0.686 0.200 0.114 0.886 1.000 0.686 0.886 0.486 0.343 0.886 0.486 0.343 0.343 0.343 0.200 0.114 0.200 0.114 0.686 0.486 0.886 0.343 0.486 0.486 0.886 0.686 0.114 0.114 0.486 0.486
p< 0 .0 5
Page A43 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
CO2 production
T im e
(h r) 6.1 7 .4 8.6 9.9 11.1 1 2 .4 13.6 14.9 16.1 1 7 .4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M eta bolic ca g e s g ro u p 1 : H F control
V C O 2 (m l/h/kg m ouse)
cage 0101 cage 0105 cage 0201 cage 0205
2929
3110
3620
2845
2978
3408
3399
2787
2781
3142
3042
2814
2963
3025
3464
2655
2773
2718
2664
2841
2721
2759
3107
2565
2478 2629
2619 2308
2736 2708
2379 2391
2418
2501
2567
2267
2713
2207
2650
2315
3008
2361
2357
2233
2486
2419
2757
2474
2362 2412
2465 2231
2357 2731
2041 2440
2563
2617
2602
2402
3454
3237
3386
3369
2775
2971
3388
3044
3139
3105
3283
2578
2804
2718
3414
3120
2860 2861
2620 2568
3100 3243
2592 2750
2563
2568
3137
2730
2599
2436
2976
2197
2801
2505
2827
2280
2500
2421
2436
2334
2517
2267
2933
2241
2627 2470
2362 2518
2813 2873
2191 2345
2748
2440
2656
2095
2281
2470
2655
2354
2448
2244
2505
2168
2369
2400
2829
2209
2594 2662
2551 2631
2874 2543
2334 2510
3258
3226
3635
3092
3054
2903
3311
2877
3010
3083
3407
2777
2744
3012
3274
2839
2610
2778
2975
2727
2547 2403
2717 2640
3158 2997
2521 2341
2581
2784
3114
2383
2571
2393
2915
2423
2631
2574
2798
2088
2574
2152
2597
2128
2295
2478
2429
1822
2211 2536
2122 2019
2445 2461
1985 1914
2132
1693
1936
2008
2274
2161
2434
1765
1943
2025
2320
2073
1969
2239
2420
1970
2228
1950
2546
2267
2767 2844
2843 3169
3065 3474
2691 2866
2927
3054
3241
2818
2559
2840
3150
2531
2530
2822
2961
2683
Average VCO2
(ml/h/kg mouse) 3126 3143 2945 3027 2749 2788 2553 2509 2438 2471 2490 2534 2306 2453 2546 3361 3044 3026 3014 2793 2856 2750 2552 2603 2423 2490 2498 2552 2485 2440 2341 2451 2588 2587 3303 3036 3069 2967 2773 2736 2595 2715 2576 2523 2363 2256 2191 2232 1943 2159 2090 2150 2248 2841 3088 3010 2770 2749
SD
348 311 176 333 76 229 157 190 130 248 351 152 184 207 99 91 256 309 318 238 285 270 327 260 68 321 276 227 290 163 161 265 222 71 233 199 261 233 152 295 297 312 239 305 258 299 193 312 185 285 162 221 244 162 297 182 289 185
T im e
(h r) 6.1 7.4 8.6 9.9 11.1 1 2 .4 13.6 14.9 16.1 1 7 .4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M eta bolic ca g e s g ro u p 3: H F + 0 .03 % P F B S
V C O 2 (m l/h/kg m ouse)
Average VCO2
cage 0102 cage 0106 cage 0202 cage 0206
3252
3003
2825
3076
3223
3131
3321
3262
2920
2824
3352
2920
2958
2601
2985
2986
3064
2849
3475
2676
2699
2726
3161
2520
2801
2151
2872
2743
2572
2481
2697
2060
2714
2400
3020
2190
2585
2148
3083
2324
2574
2524
2658
2156
2164
2181
2852
2015
2411
2426
3150
2209
2300
2522
2630
2256
2736
2188
3094
2372
3201
3308
3794
3129
3254
3232
3611
3130
2985
2775
3335
3196
3014
2175
3300
2743
2775
2675
3305
2895
2439
1920
3161
2671
2504
2269
3092
2606
2610
2010
2889
2378
2617
2140
2941
2590
2477
2465
2788
2208
2899
1937
2859
2524
2685
2169
3039
2355
2683
2199
3043
2262
2621
2408
2877
2293
2474
1986
2836
2555
2507
2197
2699
2100
2548
2105
2784
2263
2467
2244
2683
2211
2764
2233
3009
2456
3042
2866
3422
3123
3429
3157
3477
3172
3211
3074
3245
2974
3047
2715
2949
2936
3111
2508
3013
2564
2896
2513
3032
2765
2872
2595
2781
2578
2476
2380
2820
2651
2685
2602
2998
2616
2488
2275
2807
2394
2467
2477
2587
2067
2183
2398
2543
2186
1944
2326
2532
1776
1948
2041
2791
2344
2018
2013
2171
1721
1832
1903
2347
2267
1932
2169
2213
1916
1855
1854
2316
1905
2156
1965
2756
2343
2564
2510
2797
2630
3132
2821
3506
2895
2833
2869
3164
2960
2774
2421
3060
2920
2464
2451
2963
2853
(ml/h/kg mouse) 3039 3234 3004 2883 3016 2776 2642 2452 2581 2535 2478 2303 2549 2427 2598 3358 3307 3073 2808 2913 2548 2618 2472 2572 2485 2555 2562 2547 2550 2463 2376 2425 2401 2615 3113 3309 3126 2912 2799 2802 2707 2582 2725 2491 2399 2328 2145 2281 1981 2087 2057 1982 2305 2625 3088 2956 2794 2683
SD
177 80 236 188 345 272 331 276 363 407 222 374 413 179 402 300 210 245 480 277 515 346 372 329 237 445 383 394 257 354 276 301 219 341 232 168 125 140 307 221 144 194 186 228 228 176 346 380 188 257 155 224 338 125 308 149 275 264
Page A44 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
T im e
(h r) 6.1 7 .4 8.6 9.9 11.1 1 2 .4 13.6 14.9 16.1 1 7 .4 18.6 19.9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M e ta bolic ca g e s g ro u p 4: H F + 0 .0 0 6 % P F H S
V C O 2 (m l/h/kg m ouse)
cage 0103 cage 0107 cage 0203 cage 0207
3309
3390
3535
3348
3243 2910
3195 3285
3526 3487
3375 2961
3013
2980
3360
2695
2848
2670
3385
2981
2803
3043
3222
3000
2757
2340
2802
2483
2834
2384
2957
2469
2699
2685
2527
2316
2641 2474
2677 2658
2628 2505
2448 2519
2501
3011
2615
2533
2834
2835
2489
2379
2638
2685
2634
2873
2739
2772
2653
2586
3504
3473
3331
3246
3532
3535
3688
3585
3236 3243
3539 3256
3498 3755
3374 3475
2940
3382
3493
3025
3109
2912
3516
3001
2575
2599
3708
2979
2536
2804
3465
2755
2419
1953
3136
2496
2531
2461
2999
2664
2613 2766
2818 2612
3063 2756
2466 2243
2533
2538
2510
2677
2656
2834
2715
2186
2367
2515
2371
2250
2316
2481
2372
2195
2273
2767
2861
2035
2587
2927
2695
2742
2771 3419
2605 3205
2987 3237
2323 2691
3884
3492
3775
3089
3549
3283
4014
3281
3537
3116
3646
3478
3286
3411
3615
3060
3013
2658
3123
3020
2856
2761
2950
3005
2482 2653
2458 2516
3004 2972
2631 2505
2446
2003
2852
2388
2471
2132
2679
2317
2444
2248
2508
2381
2317
1959
2651
2239
2384
2357
2281
2202
2092
1863
2403
2467
2089 2019
1983 2148
2360 2535
2074 2135
2254
2256
2476
2241
2189
2394
2578
2122
2792
2665
2648
2485
3502
3130
3196
2820
3708
2929
3755
3224
3218
2380
3623
3083
3192
2698
3327
2906
Average VCO2
(ml/h/kg mouse) 3395 3335 3161 3012 2971 3017 2596 2661 2557 2598 2539 2665 2634 2708 2687 3389 3585 3412 3432 3210 3135 2965 2890 2501 2664 2740 2594 2564 2598 2376 2341 2484 2738 2672 3138 3560 3532 3444 3343 2954 2893 2644 2662 2422 2400 2395 2292 2306 2206 2127 2209 2307 2321 2648 3162 3404 3076 3031
SD
99 148 274 273 304 172 221 278 179 102 81 236 236 113 84 121 73 137 240 269 267 528 401 487 239 259 245 76 284 109 119 395 142 280 312 355 345 230 233 204 108 252 217 348 232 111 285 82 281 163 225 113 207 126 280 397 518 283
T im e
(h r) 6.1 7 .4 8.6 9.9 11.1 1 2 .4 1 3 .6 1 4 .9 16.1 1 7 .4 1 8 .6 1 9 .9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M e ta bolic ca g e s g ro u p 6 : H F + 0 .0 0 3 % P F O S
V C O 2 (m l/h/kg m ouse)
| Average VCO2
c a g e 0 1 0 4 c a g e 0 1 0 8 c a g e 0 2 0 4 c a g e 0 2 0 8 (ml/h/kg mouse)
3618
3732
3629
3220
3550
3414
3149
3553
3346
3365
3077
3062
3321
2963
3106
3331
3294
3219
3015
3215
3459
3188
3324
2801
3193
3301
2650
2970
2656
2894
3344
2303
2875
2554
2769
3167
2783
2760
2326
2759
2754
2270
2407
2550
2495
2745
2591
2365
2139
2460
3329
2992
2511
2371
2801
3154
2288
2097
2433
2493
2765
2771
2441
2405
2595
2658
2817
2364
2466
2576
3248
2519
2387
2668
2705
3643
3328
2734
2974
3170
3711
3457
3256
3206
3408
3410
3409
3637
3476
3483
3586
3714
3225
3340
3466
3324
3143
3102
2929
3124
3436
3095
3183
2900
3153
3213
2431
3022
2895
2890
3040
2809
3004
2693
2887
3259
2784
2951
2688
2921
3172
2333
2737
2351
2648
3179
2503
2817
2633
2783
3080
2433
2362
2717
2648
2992
2068
2449
2838
2587
2701
2306
2399
2627
2508
2828
2532
2224
2310
2474
2695
2234
2681
2499
2527
2775
2233
2426
2409
2461
2741
2411
2500
2573
2556
2766
2411
2616
2534
2582
3454
2739
3044
2867
3026
3931
3266
3167
3104
3367
3393
3634
3407
3247
3420
3656
3495
3236
3014
3350
3109
3309
3021
3121
3140
3360
2940
2868
2811
2995
3313
2510
2896
2675
2849
3183
2708
2683
2661
2809
3210
2732
2631
2576
2787
3309
2775
2552
2529
2791
3020
2472
2453
2522
2617
2882
2549
2424
2522
2594
2636
2062
2504
2233
2359
2675
2302
2208
2333
2379
2419
2040
2197
2131
2197
2724
2379
2060
2004
2291
2533
2023
2195
1971
2180
2450
2344
2016
2234
2261
2505
2242
1969
2016
2183
3034
2294
2265
2426
2505
3260
3089
2531
2702
2895
3398
2900
2924
3037
3065
3198
3204
3044
3244
3172
3051
3148
2734
3064
2999
SD
226 168 152 141 284 310 449 344 207 265 441 462 200 201 380 399 230 107 224 162 223 333 165 251 396 294 326 414 186 270 215 227 140 149 312 382 160 283 121 249 348 250 289 363 270 199 260 204 161 332 254 185 246 360 337 230 88 182
Page A45 of A172
Appendix to TNO-report of 3M-02_____________ Final version
Time ( hr)
6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p
4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.486 4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.486 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.114 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.114 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.886
gr
n
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
(/) >
p
0.343 0.686 0.343 1.000 0.200 0.200 0.686 0.486 0.343 0.889 0.343 0.200 0.057 0.200 0.114 0.686 0.029 0.057 0.114 0.114 0.200 0.489 0.343 0.686 0.057 0.343 0.886 0.486 0.486 0.886 1.000 1.000 0.343 0.686 0.686 0.057 0.114 0.057 0.029 0.486 0.200 0.886 0.486 0.686 0.886 0.686 0.686 1.000 0.343 0.686 0.486 0.200 0.886 0.114 0.886 0.200 0.486 0.200
VCO2, p-values Mann Whitney U-test
group 1 vs 6
nn E
4 0.114
group 3 vs 4 group 3 vs 6
3A Y4nnI
n
p n0 .(0 2. 9
4
0.057
4
0.343 4
0.486 4
0.200
4
0.343 4
0.486 4
0.343
4
0.486 4
0.486 4
0.029
4
0.057 4
0.686 4
0.686
4
0.886 4
0.200 4
0.886
4
0.686 4
0.886 4
0.686
4
0.200 4
0.686 4
0.200
4
0.886 4
0.886 4
1.000
4
1.000 4
0.486 4
1.000
4
0.200 4
0.686 4
0.686
4
0.486 4
0.200 4
0.486
4
0.114 4
0.686 4
0.686
4
0.486 4
0.029 4
0.343
4
0.886 4
0.686 4
0.686
4
0.486 4
0.486 4
0.686
4
0.114 4
0.200 4
0.486
4
0.029 4
0.057 4
0.029
4
0.114 4
0.114 4
0.057
4
0.057 4
0.114 4
0.200
4
0.200 4
0.200 4
0.114
4
0.686 4
0.486 4
0.486
4
0.114 4
0.343 4
0.114
4
0.343 4
0.686 4
0.114
4
0.886 4
0.486 4
0.886
4
0.343 4
0.886 4
0.886
4
0.486 4
0.886 4
0.486
4
1.000 4
1.000 4
1.000
4
0.886 4
0.886 4
1.000
4
1.000 4
0.486 4
0.886
4
0.343 4
0.886 4
0.686
4
0.686 4
0.886 4
1.000
4
0.886 4
0.057 4
0.343
4
0.886 4
0.886 4
1.000
4
0.200 4
0.886 4
0.886
4
0.200 4
0.343 4
0.886
4
0.114 4
0.029 4
0.029
4
0.114 4
0.029 4
0.057
4
0.029 4
0.057 4
0.114
4
0.200 4
0.486 4
0.486
4
0.343 4
0.200 4
0.686
4
0.686 4
0.886 4
0.200
4
0.200 4
0.486 4
0.886
4
0.886 4
0.886 4
0.200
4
0.686 4
1.000 4
0.686
4
0.114 4
0.686 4
0.114
4
0.343 4
0.686 4
0.486
4
0.686 4
0.686 4
0.886
4
0.114 4
0.343 4
0.114
4
0.886 4
0.686 4
0.343
4
0.886 4
0.686 4
0.486
4
0.486 4
0.200 4
0.114
4
0.886 4
0.886 4
0.886
4
0.200 4
0.886 4
0.343
4
0.486 4
1.000 4
0.486
4
1.000 4
0.114 4
0.486
4
0.057 4
0.343 4
0.057
4
0.114 4
0.200 4
0.114
group 4 vs 6
0.343 0.886 0.886 0.343 0.486 0.486 0.686 1.000 0.886 0.486 0.686 0.343 0.686 0.486 0.686 0.486 0.343 0.686 1.000 0.686 1.000 1.000 0.686 0.200 0.886 0.886 1.000 1.000 0.486 0.886 0.200 1.000 0.114 0.686 0.886 0.886 0.886 0.886 0.343 0.886 0.686 0.200 0.486 0.200 0.343 0.114 1.000 0.886 1.000 0.686 0.886 0.686 0.486 0.343 0.343 0.200 1.000 1.000
04-12-2006 p< 0.05
Page A46 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Respiratory exchange ratio
Time
Jhr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1: HF control
Respiratory exchange ratio
Average
cage 0101 cage 0105 cage 0201 cage 0205
RER
0.960 0.861
1.015
0.895
0.933
1.025
0.902
1.037
0.934
0.974
0.997
0.900
0.994
0.931
0.955
0.971
0.854
0.962
0.909
0.924
1.014
0.848
0.906
0.884
0.913
0.966
0.933
0.975
0.885
0.940
0.939 0.841
1.000
0.892
0.918
0.981
0.839
0.976
0.883
0.920
1.008
0.890
0.926
0.845
0.917
0.962
0.817
0.917
0.837
0.883
0.969
0.824
0.905
0.815
0.878
0.896
0.827
0.936
0.792
0.863
0.906
0.821
0.895
0.803
0.856
0.877
0.824
0.966
0.843
0.878
0.852
0.876
0.867
0.862
0.864
0.908
0.878
0.890
0.857
0.883
0.941
0.886
0.960
0.785
0.893
0.923
0.877
0.936
0.853
0.897
0.965
0.823
1.007
0.877
0.918
0.963
0.802
0.995
0.866
0.906
0.961
0.784
0.977
0.903
0.906
0.926
0.805
0.952
0.856
0.885
0.948
0.765
0.876
0.747
0.834
0.889
0.809
0.881
0.797
0.844
0.879
0.780
0.907
0.870
0.859
0.882
0.764
0.894
0.821
0.840
0.985
0.920
0.998
0.865
0.942
1.030
0.936
1.040
0.881
0.972
1.027
0.916
1.012
0.856
0.953
0.972
0.882
0.947
0.827
0.907
0.962
0.890
0.946
0.814
0.903
0.930
0.892
0.985
0.863
0.917
0.940
0.882
0.959
0.845
0.906
0.905
0.920
0.941
0.908
0.918
0.913
0.916
0.968
0.915
0.928
0.881
0.886
0.881
0.856
0.876
0.907
0.888
0.964
0.901
0.915
0.919
0.870
0.981
0.910
0.920
0.892
0.870
0.923
0.871
0.889
0.832
0.827
0.975
0.812
0.861
0.907
0.816
0.943
0.828
0.874
0.916
0.865
0.956
0.813
0.887
0.910
0.831
0.888
0.781
0.853
0.903
0.817
0.899
0.787
0.851
0.964
0.763
0.931
0.802
0.865
0.963
0.861
0.875
0.766
0.867
0.896
0.774
0.830
0.772
0.818
0.933
0.784
0.817
0.759
0.823
0.890
0.745
0.810
0.783
0.807
0.876
0.794
0.857
0.721
0.812
0.811
0.758
0.907
0.772
0.812
0.777
0.839
0.926
0.785
0.832
0.852
0.775
0.903
0.830
0.840
0.887
0.901
0.912
0.814
0.878
0.855
0.909
0.890
0.831
0.871
0.891
0.921
0.931
0.854
0.899
0.870
0.873
0.930
0.866
0.885
0.888
0.844
0.872
0.864
0.867
SD
0.069 0.067 0.048 0.054 0.072 0.041 0.068 0.070 0.069 0.068 0.073 0.065 0.052 0.063 0.010 0.022 0.079 0.039 0.083 0.089 0.087 0.067 0.095 0.047 0.055 0.060 0.062 0.076 0.081 0.066 0.067 0.053 0.052 0.016 0.027 0.013 0.033 0.046 0.025 0.076 0.062 0.062 0.058 0.059 0.097 0.080 0.059 0.077 0.062 0.070 0.067 0.069 0.053 0.044 0.035 0.035 0.030 0.018
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
Respiratory exchange ratio
Average
cage 0102 cage 0106 cage 0202 cage 0206
RER
0.937 0.894 0.893 0.878
0.900
0.950 0.919 0.987 0.903
0.940
0.959
0.882
0.972
0.887
0.925
0.931
0.884
0.959
0.856
0.908
0.944 0.910 0.909 0.842
0.901
0.922
0.889
0.932
0.869
0.903
0.920 0.800 0.928 0.895
0.886
0.923 0.867 0.867 0.805
0.866
0.946
0.831
0.908
0.851
0.884
0.927 0.808 0.874 0.841
0.862
0.836 0.832 0.863 0.807
0.834
0.831
0.804
0.916
0.803
0.839
0.855
0.863
0.921
0.763
0.851
0.833 0.858 0.847 0.780
0.830
0.858 0.846 0.874 0.849
0.857
0.834 0.878 0.913 0.871
0.874
0.894 0.812 0.895 0.818
0.855
0.901
0.856
0.943
0.875
0.894
0.887
0.809
0.982
0.893
0.892
0.858
0.771
0.975
0.855
0.865
0.851
0.743
0.896
0.855
0.837
0.877
0.783
0.862
0.859
0.845
0.871
0.744
0.867
0.823
0.826
0.850
0.779
0.842
0.830
0.825
0.837 0.802 0.835 0.801
0.819
0.922
0.765
0.852
0.871
0.852
0.994 0.868 0.960 0.929
0.938
0.988 0.870 0.957 0.917
0.933
0.998 0.920 0.955 0.904
0.944
0.949
0.901
0.864
0.811
0.881
0.916
0.931
0.829
0.766
0.860
0.912
0.842
0.825
0.824
0.851
0.912
0.851
0.833
0.821
0.854
0.906 0.883 0.900 0.893
0.895
0.854 0.919 0.888 0.861
0.881
0.890
0.902
0.902
0.809
0.876
0.929
0.892
0.882
0.845
0.887
0.958
0.907
0.892
0.893
0.912
0.928 0.895 0.873 0.808
0.876
0.938 0.875 0.853 0.840
0.877
0.930 0.855 0.814 0.809
0.852
0.864 0.826 0.829 0.812
0.833
0.870
0.829
0.832
0.790
0.830
0.815
0.800
0.811
0.783
0.802
0.922
0.786
0.840
0.784
0.833
0.884 0.858 0.880 0.803
0.856
0.809
0.860
0.861
0.746
0.819
0.833 0.854 0.863 0.801
0.838
0.793 0.877 0.770 0.719
0.790
0.759 0.822 0.778 0.790
0.787
0.783 0.814 0.789 0.760
0.787
0.754
0.758
0.811
0.767
0.772
0.794
0.844
0.841
0.835
0.828
0.787 0.873 0.794 0.830
0.821
0.894
0.867
0.862
0.809
0.858
0.879 0.856 0.875 0.854
0.866
0.892
0.826
0.893
0.850
0.866
0.821
0.826
0.826
0.853
0.831
SD
0.025 0.037 0.047 0.046 0.042 0.029 0.059 0.048 0.053 0.051 0.023 0.053 0.065 0.034 0.013 0.032 0.046 0.037 0.071 0.084 0.065 0.042 0.059 0.032 0.020 0.065 0.053 0.051 0.042 0.059 0.077 0.041 0.040 0.010 0.030 0.045 0.034 0.031 0.051 0.044 0.056 0.022 0.033 0.014 0.065 0.037 0.054 0.027 0.066 0.027 0.022 0.026 0.023 0.039 0.036 0.013 0.033 0.014
Page A47 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
T im e (h r) 6.1 7 .4 8 .6 9 .9 11.1 1 2 .4 1 3 .6 1 4 .9 16.1 1 7 .4 1 8 .6 1 9 .9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M e ta b o lic c a g e s g ro u p 4: H F + 0 .0 0 6 % P F H S
R e s p ira to ry e x c h a n g e ratio
Average
cage 0103 cage 0107 cage 0203 cage 0207
RER
0 .9 2 7
0 .9 1 8
0 .9 3 2
0 .9 1 3
0.922
0 .9 4 4
0 .9 2 5
0 .9 4 2
0 .9 4 4
0.939
0 .8 8 9
0 .9 2 0
0 .9 3 8
0 .9 2 2
0.917
0 .9 1 3
0 .9 0 5
0 .9 0 7
0 .8 9 4
0.905
0 .8 8 5
0.881
0 .8 9 6
0 .9 2 0
0.895
0 .8 5 8
0 .8 8 9
0 .8 7 6
0 .8 9 5
0.880
0 .8 4 8
0 .8 4 3
0 .8 6 9
0 .8 5 2
0.853
0.901
0 .8 1 7
0.861
0 .8 6 0
0.860
0 .8 6 2
0.791
0 .7 9 5
0 .8 0 2
0.812
0.851
0 .8 0 7
0 .8 2 3
0 .8 8 4
0.841
0 .8 2 0
0 .9 1 8
0 .8 4 3
0 .8 3 7
0.854
0 .8 1 0
0 .9 5 2
0 .8 0 3
0 .8 8 4
0.862
0 .9 1 9
0 .9 4 3
0 .8 4 2
0 .8 3 9
0.886
0 .8 6 3
0 .9 0 5
0 .8 2 4
0 .8 5 7
0.862
0 .8 4 5
0 .8 8 0
0 .8 0 2
0 .8 4 5
0.843
0 .8 6 8
0 .7 8 7
0 .8 7 9
0 .8 8 8
0.855
0 .8 2 9
0 .8 2 8
0 .8 7 5
0.871
0.851
0 .8 5 5
0 .9 1 2
0 .9 1 2
0 .8 9 9
0.894
0 .9 2 2
0 .9 6 8
0 .9 4 8
0 .9 2 6
0.941
0 .9 3 3
0 .9 5 9
0 .9 6 0
0 .9 3 4
0.947
0 .9 1 5
0 .9 1 9
0 .9 6 3
0 .9 3 2
0.932
0 .8 1 5
0 .9 1 2
0 .9 3 0
0 .9 1 6
0.893
0 .7 9 5
0 .8 7 2
0 .8 8 2
0 .8 3 3
0.846
0 .8 1 3
0 .8 0 6
0 .8 7 0
0 .8 1 2
0.825
0 .7 9 4
0.851
0 .8 9 6
0.801
0.836
0 .8 6 6
0 .9 3 8
0 .8 6 6
0 .7 4 9
0.855
0 .9 4 2
0 .9 3 9
0 .8 5 6
0 .8 4 5
0.895
0 .8 9 0
0 .9 4 5
0 .8 1 2
0 .9 2 9
0.894
0 .9 0 3
0 .9 6 8
0 .8 4 3
0 .8 6 7
0.895
0 .8 1 2
0 .9 1 9
0 .7 9 5
0.821
0.837
0.781
0 .9 2 5
0 .7 9 6
0 .7 9 4
0.824
0 .8 1 3
0 .9 2 3
0 .8 2 9
0 .7 5 4
0.830
0 .8 4 0
0 .8 9 2
0 .8 8 4
0 .8 5 7
0.868
0 .8 6 0
0 .9 1 0
0 .9 1 5
0.861
0.887
0 .9 2 5
0 .9 1 3
0 .9 2 4
0.871
0.908
0 .9 2 2
0 .8 8 2
0.921
0 .8 3 9
0.891
0 .9 3 9
0 .8 9 0
0 .9 2 2
0 .8 8 5
0.909
0 .9 6 4
0 .9 1 9
0.931
0 .9 2 6
0.935
0 .9 4 5
0 .9 5 8
0 .9 4 0
0 .9 2 2
0.941
0 .9 0 0
0 .9 0 9
0 .8 4 0
0 .9 0 8
0.889
0 .8 6 3
0 .8 9 8
0 .8 3 0
0 .8 9 3
0.871
0 .8 1 9
0 .8 1 9
0 .8 0 3
0 .8 6 2
0.826
0 .8 1 4
0.801
0 .8 0 3
0 .7 9 2
0.803
0 .8 2 3
0 .7 7 7
0 .8 2 9
0 .8 1 0
0.810
0 .8 2 0
0 .8 0 3
0 .8 2 8
0 .8 3 9
0.822
0 .8 0 9
0 .7 8 6
0 .8 3 4
0 .8 4 9
0.819
0.801
0 .7 7 7
0 .7 9 5
0 .8 3 8
0.803
0 .8 1 7
0.811
0 .7 4 5
0 .8 3 2
0.801
0 .8 0 2
0.781
0 .7 5 3
0 .8 0 6
0.786
0 .7 5 4
0 .7 9 8
0 .7 8 5
0.771
0.777
0 .7 5 4
0 .7 8 6
0 .8 2 0
0 .7 9 6
0.789
0 .7 8 9
0 .7 6 9
0 .8 1 9
0 .8 0 3
0.795
0 .7 6 3
0 .8 0 2
0 .8 4 6
0 .8 0 9
0.805
0 .8 6 2
0 .8 0 9
0 .8 0 4
0 .8 5 0
0.831
0 .9 2 4
0.781
0.841
0 .8 2 2
0.842
0 .9 3 9
0 .7 8 9
0 .8 7 7
0 .8 4 6
0.863
0 .9 5 5
0 .7 9 9
0 .8 7 8
0 .8 8 8
0.880
0 .9 3 6
0 .8 3 6
0.841
0 .8 6 0
0.868
SD
0.008 0.009 0.020 0.008 0.018 0.016 0.012 0.034 0.034 0.034 0.044 0.070 0.053 0.033 0.032 0.046 0.026 0.027 0.021 0.015 0.022 0.053 0.040 0.030 0.048 0.078 0.052 0.059 0.055 0.056 0.068 0.070 0.024 0.030 0.025 0.040 0.026 0.020 0.015 0.033 0.031 0.025 0.009 0.023 0.015 0.028 0.026 0.039 0.025 0.019 0.027 0.021 0.034 0.029 0.060 0.063 0.064 0.047
T im e (h r) 6.1 7 .4 8.6 9.9 11.1 1 2 .4 1 3 .6 1 4 .9 16.1 1 7 .4 1 8 .6 1 9 .9 21.1 2 2 .4 2 3 .6 2 4 .9 26.1 2 7 .4 2 8 .6 2 9 .9 31.1 3 2 .4 3 3 .6 3 4 .9 36.1 3 7 .4 3 8 .6 3 9 .9 41.1 4 2 .4 4 3 .6 4 4 .9 46.1 4 7 .4 4 8 .6 4 9 .9 51.1 5 2 .4 5 3 .6 5 4 .9 56.1 5 7 .4 5 8 .6 5 9 .9 61.1 6 2 .4 6 3 .6 6 4 .9 66.1 6 7 .4 6 8 .6 6 9 .9 71.1 7 2 .4 7 3 .6 7 4 .9 76.1 7 7 .4
M e ta b o lic c a g e s g ro u p 6 : H F + 0 .0 0 3 % P F O S
R e s p ira to ry e x c h a n g e ratio
| Average
cage 0104 cage 0108 cage 0204 cage 0208
RER
0 .9 2 0
0 .8 6 3
0 .9 1 4
0 .8 8 8
0.896
0 .9 0 3
0 .7 9 0
0 .9 3 5
0 .9 1 6
0.886
0 .9 2 3
0 .8 0 2
0 .9 0 3
0 .8 7 8
0.876
0 .9 0 0
0.871
0 .8 9 9
0 .8 7 7
0.887
0 .8 9 4
0 .8 7 4
0.911
0.891
0.892
0 .9 0 9
0.821
0 .8 8 7
0 .8 5 0
0.867
0 .8 8 8
0.741
0 .8 9 3
0 .7 9 9
0.830
0 .8 8 2
0 .7 9 6
0 .8 4 8
0 .7 7 4
0.825
0 .8 2 6
0 .7 8 2
0 .7 7 7
0 .8 3 9
0.806
0 .8 5 5
0 .8 1 7
0 .7 8 7
0.771
0.808
0.891
0 .8 5 0
0 .7 8 2
0 .8 1 4
0.834
0 .8 5 3
0 .7 7 2
0 .7 1 8
0 .8 0 5
0.787
0 .8 3 5
0 .8 9 5
0 .8 2 8
0 .7 8 5
0.836
0 .8 0 6
0 .8 4 5
0 .7 9 3
0 .8 1 8
0.815
0 .8 6 8
0 .7 8 4
0.771
0 .8 3 0
0.813
0 .8 9 2
0 .8 2 8
0 .8 1 4
0 .8 7 5
0.852
0.851
0.871
0 .8 0 4
0 .8 4 8
0.844
0 .9 0 8
0.881
0 .8 7 9
0 .8 8 6
0.888
0.911
0 .9 1 4
0 .8 9 4
0 .8 8 5
0.901
0 .8 8 6
0 .9 0 0
0 .9 0 9
0 .8 4 8
0.885
0 .8 6 8
0 .8 4 0
0 .9 1 2
0 .8 6 2
0.871
0 .8 4 3
0 .7 6 4
0 .8 8 8
0 .8 2 7
0.830
0 .7 7 6
0 .8 1 9
0 .8 3 6
0 .8 1 2
0.811
0 .8 3 6
0 .8 1 2
0 .8 6 5
0 .8 3 0
0.836
0 .8 2 4
0 .7 5 6
0 .8 2 8
0.761
0.792
0 .8 9 0
0 .8 2 5
0 .8 4 7
0 .8 3 9
0.850
0 .9 2 9
0 .8 0 7
0.801
0 .8 6 9
0.852
0 .9 1 6
0 .7 5 9
0 .8 2 8
0 .9 0 2
0.851
0 .8 6 7
0 .8 1 3
0 .8 0 7
0.871
0.839
0 .8 9 8
0 .7 8 4
0 .7 9 3
0 .7 9 0
0.816
0 .8 0 8
0 .7 8 7
0 .8 4 6
0 .7 8 2
0.806
0 .8 1 6
0 .7 8 5
0 .8 3 0
0 .8 2 5
0.814
0 .8 2 7
0 .8 2 0
0 .8 0 4
0 .8 3 4
0.821
0 .8 3 6
0 .8 2 6
0 .8 8 5
0 .8 2 2
0.842
0 .8 9 6
0 .8 2 7
0 .8 5 9
0 .8 8 3
0.866
0 .8 9 0
0 .8 6 4
0 .8 6 0
0 .8 5 7
0.868
0 .8 8 5
0 .9 0 9
0.891
0 .8 3 6
0.880
0 .9 0 5
0.901
0 .8 9 7
0 .8 3 7
0.885
0 .8 2 9
0 .8 8 5
0 .8 6 0
0.861
0.859
0 .8 6 0
0 .8 5 2
0 .8 1 3
0.841
0.841
0 .8 3 5
0 .7 9 2
0 .7 9 9
0 .7 8 4
0.802
0 .8 5 2
0 .7 9 5
0 .7 4 3
0 .7 6 8
0.790
0 .8 4 6
0 .8 1 7
0 .7 7 9
0 .7 5 0
0.798
0 .8 5 5
0 .8 3 4
0 .7 3 5
0 .7 4 5
0.792
0.841
0 .7 8 3
0 .7 6 2
0 .7 8 4
0.793
0 .7 8 2
0 .8 0 3
0 .8 0 6
0 .7 9 5
0.797
0 .8 3 8
0.741
0 .8 1 3
0 .7 7 2
0.791
0 .8 3 3
0 .7 7 6
0 .8 1 6
0 .7 6 3
0.797
0 .8 2 0
0 .8 0 7
0 .7 5 3
0 .7 4 9
0.782
0 .8 3 3
0.811
0 .7 1 5
0 .7 3 6
0.774
0 .7 8 7
0 .7 5 8
0 .7 6 6
0 .7 0 9
0.755
0 .7 7 3
0 .7 9 2
0 .7 5 7
0 .7 3 3
0.764
0 .8 0 9
0 .7 8 2
0 .7 1 5
0 .7 2 2
0.757
0 .8 5 0
0 .7 8 6
0 .7 5 5
0 .7 7 5
0.792
0 .8 6 8
0 .8 3 8
0 .7 8 8
0 .8 2 5
0.830
0 .8 8 5
0.871
0 .8 3 5
0 .8 2 5
0.854
0 .8 7 6
0 .8 9 3
0 .8 7 8
0 .8 4 6
0.873
0 .8 1 4
0 .8 6 9
0.811
0 .8 3 2
0.832
SD
0.026 0.066 0.053 0.015 0.015 0.039 0.074 0.049 0.031 0.037 0.047 0.057 0.045 0.022 0.044 0.037 0.028 0.013 0.014 0.027 0.030 0.051 0.025 0.022 0.039 0.028 0.060 0.073 0.034 0.055 0.029 0.020 0.013 0.029 0.030 0.015 0.031 0.032 0.023 0.021 0.023 0.047 0.042 0.061 0.034 0.011 0.043 0.033 0.037 0.057 0.033 0.025 0.046 0.041 0.033 0.028 0.020 0.027
Page A48 of A172
Appendix to TNO-report of 3M-02_____________ Final version
04-12-2006
Time ( hr)
6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p
4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.200 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.343 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.114 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.343 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.686 4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.114 4 4 0.886 4 4 0.114 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.057
Respiratory exchange ratio, p-values Mann Whitney U-test group 1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6
nn p nn
n
4 4 [ 1.0001 4 | 4 0.686
0.343 4
p
0.886
0.200
4 I 0.886 I 4 4 0.343
1.000 4
0.343
0.057
4 [ 0.344 4 | 4 0.114
1.000 4
0.343
0.343
4 I 0.486 I 4 4 0.343
1.000 4
0.686
0.114
4 I 1.000 | 4 4 1.000
0.686 4
0.686
0.886
4 10.114 4 | 4 0.114
0.486 4
0.200
0.686
4 | 0.343 | 4 4 0.200
0.343 4
0.114
1.000
4 [ 0.344 4 | 4 0.114
0.686 4
0.343
0.343
4 | 0 .057| 4 | 4 0.029
0.114 4
0.057
0.686
4 \ 0.486 | 4 4 0.200
4 I 1.000 I 4 4 0.343
0.686 4 0.486 4
0.200 1.000
0.343 0.686
4 I 1.000 | 4 4 0.200
0.886 4
0.486
0.200
40
4 0.886
0.886 4
0.686
0.114
4 | 0 .886| 4| | 4 0.114
0.343 4
0.486
0.057
4 \ 0.343 | 4 4 0.200
0.343 4
0.200
0.343
4 [ 0.484 4 | 4 0.343
0.886 4
0.486
1.000
4 | 0.343 | 4 4 0.343
1.000 4
0.686
0.686
4 | 0.886 | 4 4 1.000
1.000 4
0.886
0.486
4 | 0 .886| 4 | 4 1.000
0.343 4
0.686
0.029
4 \ 1.000 | 4 4 0.886
0.343 4
0.686
0.029
4 | 0 .886| 4 | 4 0.486
0.029 4
0.486
0.029
4 [ 1.004 4 | 4 0.343
0.200 4
0.686
0.200
4 \ 0.886 | 4 4 1.000
0.486 4
0.486
0.343
4 | 0 .886| 4 | 4 1.000
0.886 4
1.000
0.486
4 \ 0.686 | 4 4 0.114
0.886 4
0.343
0.343
4 \ 1.000 | 4 4 0.886
1.000 4
0.686
0.686
4 | 0 .343| 4 | 4 0.200
0.343 4
0.200
0.343
4 \ 0.343 4 4 0.114
0.486 4
0.114
0.486
4 [ 0.344 4 | 4 0.114
0.200 4
0.029
0.343
4 \ 0.114 I 4 4 0.114
0.486 4
0.114
0.200
4 \ 0.114 | 4 4 0.057
0.686 4
0.486
1.000
4 [ 0.114 4 | 4 0.029
0.686 4
0.343
1.000
4 | 0.486 | 4 4 0.029
0.486 4
0.200
0.029
4 [ 0.344 4 | 4 0.029
1.000 4
0.057
0.114
4 | 0.686 | 4 4 0.029
0.200 4
0.686
0.114
4 0.486 4 4 0.886
0.686 4
0.486
0.486
4 [ 0.884 4 | 4 0.343
0.486 4
0.886
0.343
40
4 0.200
0.200 4
0.686
0.029
4 [ 0.054 4 | 4 0.114
0.057 4
0.486
0.029
4 \ 0.343 | 4 4 0.686
0.686 4
0.343
0.200
4 | 1.000 | 4 4 0.114
0.486 4
0.114
0.057
4 | 0 .200| 4| | 4 0.057
0.486 4
0.200
0.200
4 \ 0.343 | 4 4 0.200
0.343 4
0.343
1.000
4 \ 0.486 4 4 0.343
0.686 4
1.000
1.000
4 [ 1.004 4 | 4 0.343
1.000 4
0.343
0.343
4 | 0.343 | 4 4 0.343
0.200 4
0.114
0.200
4 [ 0.884 4 | 4 0.686
0.486 4
0.486
0.686
4 | 0.886 | 4 4 0.886
0.200 4
0.114
1.000
4 | 0.686 | 4 4 0.886
0.886 4
1.000
0.886
4 [ 0.484 4 | 4 0.486
0.686 4
0.886
1.000
4 | 0.886 | 4 4 0.200
0.886 4
0.200
0.343
4 [ 0.684 4 | 4 0.114
0.200 4
0.886
0.200
4 | 0.343 | 4 4 0.114
0.686 4
0.057
0.200
4 | 0.114 | 4 4 0.057
0.686 4
0.200
0.200
4 [ 0.484 4 | 4 0.200
0.686 4
0.486
1.000
4 | 0.486 | 4 4 0.114
0.886 4
0.686
0.886
4 [ 0.684 4 | 4 0.886
4 I 0.486 I 4 4 0.114
1.000 4 0.114 4
0.886 0.886
0.886 0.200
I
I p<0 05 I
Page A49 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
D6. Energy expenditure
Tim e
(hr)
6.1
7.4
8.6
9.9
11.1
12.4 13.6 14.9 16.1 17.4 18.6 19.9
21.1
22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9
66.1
67.4
68.6
69.9 71.1 72.4 73.6 74.9 76.1 77.4
M e ta b o lic c a g e s g ro u p 1: H F c o n tro l
E nergy expenditure (kcal/h/kg m ouse)
A v e ra g e e n e rg y e x p e n d itu re
cage 0101 cage 0105 cage 0201 cage 0205
(k c a l/h /k g m o u se)
15.26
17.60
18.09
15.65
1 6 .6 5
14.77
18.58
16.69
14.83
1 6 .2 2
14.06
17.22
15.46
15.02
1 5 .4 4
15.29
17.23
18.03
14.38
1 6 .2 3
13.87
15.56
14.51
15.73
1 4 .9 2
14.09
14.71
16.01
14.24
1 4 .7 6
13.11
15.06
13.84
13.08
1 3 .7 7
13.48
13.36
13.89
13.27
1 3 .5 0
12.13
13.82
13.73
13.00
1 3 .1 7
14.07 15.59
13.01 13.78
14.23
12.86
13.37 13.16
1 3 .6 7 1 3 .8 5
13.62 12.84
14.11 14.44
14.61 12.93
14.87
12.21
1 4 .3 0 1 3 .1 1
13.44
13.08
14.15
14.04
1 3 .6 8
14.61
14.60
14.57
13.58
1 4 .3 4
18.76
18.08
18.68
19.18
1 8 .6 7
14.71
16.46
17.67
18.47
1 6 .8 3
16.80
17.25
17.38
14.72
1 6 .5 4
14.60
15.94
17.12
17.36
1 6 .2 5
14.85
15.69
15.70
14.61
1 5 .2 1
14.90
15.61
16.65
14.99
1 5 .5 4
13.70
15.30
16.48
15.57
1 5 .2 6
13.66
15.14
16.64
13.85
1 4 .8 2
15.51
14.87
15.70
13.65
1 4 .9 3
13.93
14.80
13.31
13.10
1 3 .7 8
13.90
14.00
16.10
13.12
1 4 .2 8
13.43
12.20
12.69 13.39
14.25 14.13
12.34 13.03
1 3 .1 8 1 3 .1 9
13.65
13.20
13.31
11.93
1 3 .0 2
11.75
13.71
13.91
13.69
1 3 .2 7
12.71
12.39
13.16
12.74
1 2 .7 5
12.64
13.18
14.44
12.48
1 3 .1 9
13.72
14.15
14.99
13.38
1 4 .0 6
14.48
14.15
13.45
13.61
1 3 .9 3
17.69
17.46
18.78
16.77
1 7 .6 7
16.95
16.08
18.41
16.32
1 6 .9 4
16.37
17.06
17.66
15.18
1 6 .5 7
14.77
16.94
16.72
15.37
1 5 .9 5
14.41
15.64
15.92
15.35
1 5 .3 3
14.84
15.90
16.22
14.90
1 5 .4 6
13.10
15.64
15.87
13.62
1 4 .5 6
13.93
15.75
16.27
14.08
1 5 .0 1
13.93
13.92
16.08
14.73
1 4 .6 7
14.30
15.21
15.35
12.69
1 4 .3 9
13.41
13.41
13.86
12.72
1 3 .3 5
11.94
14.05
13.54
11.28
1 2 .7 0
12.07 13.53
12.94 12.28
14.22 14.41
12.19
11.88
1 2 .8 6 1 3 .0 2
11.78
10.77
11.51
12.25
1 1 .5 8
12.67
12.95
13.74
11.51
1 2 .7 2
11.52
12.61
12.64
12.73
1 2 .3 7
12.09
12.89
12.94
11.99
1 2 .4 8
12.71
11.98
13.88
13.18
1 2 .9 4
15.29
15.54
16.61
15.93
1 5 .8 4
16.15
17.21
19.20
16.67
1 7 .3 1
16.12
16.43
17.30
16.02
1 6 .4 7
14.28
15.90
16.75
14.24
1 5 .2 9
14.01
16.21
16.58
15.16
1 5 .4 9
SD
1.4 0 1 .8 1 1.3 2 1.6 9 0.8 8 0.8 8 0.9 3 0.2 7 0.7 8 0.5 8 1.2 2 0.5 6 0.9 5 0 .5 1 0.5 0 0.4 5 1.6 4 1.2 4 1.2 7 0.5 7 0.8 0 1.1 6 1.3 8 0.9 3 0.7 6 1.2 8 0.8 4 0.8 0 0.7 5 1 .0 1 0.3 2 0.8 9 0.7 0 0.4 8 0.8 4 1.0 5 1.0 6 1.0 5 0.6 6 0.7 0 1.4 0 1.1 8 1.0 2 1.2 2 0.4 7 1 .3 1 0.9 9 1.1 6 0.6 2 0.9 2 0.5 7 0 .5 1 0.8 0 0.5 7 1.3 3 0.5 8 1.2 4 1.1 6
Tim e (hr)
6.1
7.4
8.6
9.9
11.1
12.4 13.6 14.9 16.1 17.4 18.6 19.9
21.1
22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9
66.1
67.4
68.6
69.9 71.1 72.4 73.6 74.9 76.1 77.4
M etabolic cages group 3: HF + 0.03 % PFBS
E nergy expenditure (kcal/h/kg m ouse)
A v e ra g e e n e rg y e x p e n d itu re
cage 0102 cage 0106 cage 0202 cage 0206
(k c a l/h /k g m o u se)
17.43
16.55
15.54
17.20
1 6 .6 8
17.09
16.86
16.94
17.80
1 7 .1 7
15.39
15.70
17.29
16.16
1 6 .1 3
15.91
14.45
15.57
16.99
1 5 .7 3
16.34
15.43
18.84
15.42
1 6 .5 1
14.64
15.03
16.85
14.16
1 5 .1 7
15.19
12.93
15.35
15.09
1 4 .6 4
13.95
13.93
15.14
12.27
1 3 .8 2
14.44
13.92
16.41
12.52
1 4 .3 2
14.01
12.76
17.22
13.38
1 4 .3 4
14.98
14.58
15.00
12.81
1 4 .3 4
12.72
13.02
15.40
12.07
1 3 .3 0
13.81
13.65
16.90
13.71
1 4 .5 2
13.46
14.25
15.08
13.72
1 4 .1 3
15.62
12.57
17.30
13.56
1 4 .7 6
18.75
18.37
20.52
17.56
1 8 .8 0
18.06
19.16
19.87
18.43
1 8 .8 8
16.48
15.80
17.65
17.88
1 6 .9 5
16.92
12.95
16.88
15.09
1 5 .4 6
15.90 14.09
16.54
12.21
17.00 17.31
16.45 15.23
1 6 .4 7 1 4 .7 1
14.12
13.77
17.36
14.77
1 5 .0 0
14.78
12.74
16.24
13.97
1 4 .4 3
15.12
13.05
16.89
15.06
1 5 .0 3
14.52 15.83
14.64
12.02
16.12 16.36
13.22 14.18
1 4 .6 2 1 4 .6 0
13.76 13.81
12.26 12.37
15.82 15.88
12.58
12.21
1 3 .6 1 1 3 .5 7
13.39
12.95
15.06
12.52
1 3 .4 8
13.12
10.84
16.01
15.10
1 3 .7 7
13.64
11.69
15.73
13.03
1 3 .5 2
13.95
12.08
16.21
13.22
1 3 .8 6
13.47
12.78
15.51
12.95
1 3 .6 8
15.18
12.38
16.46
13.52
1 4 .3 9
17.57
15.45
18.91
17.72
1 7 .4 1
19.09
17.24
18.98
18.82
1 8 .5 3
17.30
16.92
17.99
17.07
1 7 .3 2
16.05
14.75
16.27
16.18
1 5 .8 1
16.77
13.77
16.86
15.23
1 5 .6 6
15.50
14.08
17.30
15.96
1 5 .7 1
15.48
14.73
16.46
15.33
1 5 .5 0
14.11
13.92
16.45
15.71
1 5 .0 5
15.24
15.13
17.39
15.86
1 5 .9 1
14.87
13.68
16.69
14.54
1 4 .9 4
13.37
15.05
14.93
12.60
1 3 .9 9
12.14
13.59
14.17
13.05
1 3 .2 4
11.67
13.16
14.32
11.27
1 2 .6 0
11.45
11.64
15.69
13.95
1 3 .1 8
12.32 11.54
11.23
11.21
13.41 14.36
11.25 13.65
1 2 .0 5 1 2 .6 9
11.90
12.82
13.42
11.92
1 2 .5 2
11.77
11.55
13.71
11.82
1 2 .2 1
13.05
11.30
15.86
13.56
1 3 .4 4
15.71
14.02
16.76
15.30
1 5 .4 4
17.40
15.88
19.87
17.20
1 7 .5 9
15.92
16.23
17.67
16.87
1 6 .6 8
15.41
14.16
16.82
16.69
1 5 .7 7
14.65
14.28
17.27
16.29
1 5 .6 2
SD
0 .85 0 .43 0 .83 1 .0 5 1 .6 1 1 .1 7 1 .1 4 1 .1 8 1 .6 1 1 .9 9 1 .0 4 1 .4 5 1 .5 9 0 .72 2 .12 1 .2 5 0 .80 0 .98 1 .8 8 0 .45 2 .13 1 .6 2 1 .4 7 1 .5 7 1 .1 9 1 .9 5 1 .6 1 1 .7 0 1 .1 1 2 .29 1 .6 8 1 .7 4 1 .2 5 1 .7 9 1 .4 4 0 .87 0 .48 0 .7 1 1 .4 6 1 .3 3 0 .72 1 .2 3 1 .0 4 1 .2 7 1 .2 0 0 .86 1 .4 0 2 .02 1 .0 4 1 .5 5 0 .74 1 .0 0 1 .8 8 1 .1 3 1 .6 7 0 .77 1 .2 5 1 .4 0
Page A50 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Tim e
(hr)
6.1
7.4
8.6
9.9
11.1
12.4 13.6 14.9 16.1 17.4 18.6 19.9
21.1
22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9
66.1
67.4
68.6
69.9 71.1 72.4 73.6 74.9 76.1 77.4
M etabolic cages group 4: HF + 0.006 % PFHS
Energy expenditure (kcal/h/kg m ouse)
A v e ra g e en erg y exp e n d itu re
cage 0103 cage 0107 cage 0203 cage 0207
(kcal/h /kg m o u s e )
17.72
18.32
18.90
18.10
1 8 .2 6
17.11
17.10
18.69
17.78
1 7 .6 7
16.08
17.64
18.56
15.91
1 7 .0 5
16.29
16.21
18.34
14.84
1 6 .4 2
15.79
14.83
18.65
16.03
1 6 .3 3
15.90
16.76
18.05
16.46
1 6 .7 9
15.81
13.50
15.81
14.17
1 4 .8 2
15.50
14.04
16.75
14.00
1 5 .0 7
15.27
16.11
15.26
13.82
1 5 .1 2
15.08
15.83
15.47
13.59
1 4 .9 9
14.56
14.33
14.50
14.54
1 4 .4 8
14.82
15.78
15.58
14.07
1 5 .0 6
15.26
14.93
14.36
13.74
1 4 .5 7
14.90
14.62
15.33
16.29
1 5 .2 8
15.70
15.43
15.87
14.86
1 5 .4 6
19.72 20.61
21.15 20.63
18.58
20.66
17.89 20.08
1 9 .3 3 2 0 .5 0
18.46
19.15
18.98
18.45
1 8 .7 6
17.40
16.86
19.80
18.59
1 8 .1 6
15.63
17.63
18.26
16.07
1 6 .9 0
16.78
15.69
18.33
15.98
1 6 .6 9
15.19
14.09
19.84
16.07
1 6 .2 9
15.21
15.71
19.30
15.97
1 6 .5 5
14.31
11.67
17.68
14.78
1 4 .6 1
15.18
14.05
16.54
15.91
1 5 .4 2
14.73
15.03
17.36
15.56
1 5 .6 7
14.63
13.86
15.71
12.89
1 4 .2 7
13.99
13.36
14.92
14.30
1 4 .1 4
14.49
14.67
15.65
12.34
1 4 .2 9
14.01
13.51
14.35
13.17
1 3 .7 6
14.08
13.28
14.29
13.21
1 3 .7 1
13.47
14.84
16.71
12.78
1 4 .4 5
14.88
16.08
15.00
15.51
1 5 .3 7
15.64
14.14
16.17
13.12
1 4 .7 7
18.28
20.86
17.34 19.42
17.39 20.34
15.11 17.84
1 7 .0 3 1 9 .6 2
18.77
18.11
21.60
18.16
1 9 .1 6
18.37
16.76
19.49
18.59
1 8 .3 0
17.30
17.80
19.21
16.43
1 7 .6 8
16.46
14.44
18.06
16.42
1 6 .3 5
16.08
15.13
17.28
16.52
1 6 .2 5
14.64
14.47
18.02
14.88
1 5 .5 0
15.67
15.08
17.87
15.07
1 5 .9 2
14.27
12.31
16.74
14.16
1 4 .3 7
14.48
12.77
15.72
13.40
1 4 .0 9
14.56 13.88
13.63
12.02
14.62 15.96
13.60 12.96
1 4 .1 1 1 3 .7 0
13.99
13.95
14.50
12.77
1 3 .8 0
12.49
11.40
15.11
14.61
1 3 .4 0
13.11
12.66
11.93 12.96
14.36 14.99
12.75 12.77
1 3 .0 4 1 3 .3 5
13.64
13.88
14.56
13.32
1 3 .8 5
13.62
14.26
14.77
12.59
1 3 .8 1
15.79
15.84
15.90
14.20
1 5 .4 3
18.77
19.12
18.49
16.54
1 8 .2 3
19.64
17.74
21.04
18.51
1 9 .2 3
16.81
14.27
20.24
17.03
1 7 .0 9
16.94
15.58
19.19
16.40
1 7 .0 3
SD
0 .4 9 0 .7 5 1 .2 7 1 .4 4 1 .6 4 0 .9 1 1 .1 7 1 .3 2 0 .9 5 0 .9 9 0 .1 1 0 .7 8 0 .6 7 0 .7 3 0 .4 4 1 .4 3 0 .2 8 0 .3 6 1.31 1 .2 5 1 .1 8 2 .5 0 1 .8 6 2 .4 6 1 .0 7 1 .1 8 1 .2 0 0 .6 5 1 .3 9 0 .5 2 0 .5 5 1 .7 3 0 .5 5 1 .4 0 1 .3 5 1 .3 3 1 .6 5 1 .1 4 1 .1 6 1 .4 8 0 .9 0 1 .6 9 1 .3 3 1 .8 2 1 .3 0 0 .5 6 1 .6 9 0 .7 3 1 .7 5 1.01 1 .1 0 0 .5 3 0 .9 4 0 .8 2 1 .1 6 1 .4 3 2 .4 5 1 .5 5
Tim e (hr)
6.1
7.4
8.6
9.9
11.1
12.4 13.6 14.9 16.1 17.4 18.6 19.9
21.1
22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9
66.1
67.4
68.6
69.9 71.1 72.4 73.6 74.9 76.1 77.4
M etabolic cages group 6: HF + 0.003 % PFO S
Energy expenditure (kcal/h/kg m ouse)
A v e ra g e en erg y exp e n d itu re
cage 0104 cage 0108 cage 0204 cage 0208
(k c a l/h /k g m o u s e )
19.47
21.08
19.61
17.81
1 9 .4 9
18.62
19.06
18.86
18.04
1 8 .6 5
16.51
18.30
18.11
16.54
1 7 .3 6
18.20
18.49
17.62
16.84
1 7 .7 9
19.01
17.84
18.00
15.43
1 7 .5 7
17.93
15.57
16.43
15.19
1 6 .2 8
18.49
14.69
15.83
15.23
1 6 .0 6
17.61
16.67
15.79
14.34
1 6 .1 0
16.12
13.87
14.75
14.73
1 4 .8 7
15.64
15.29
14.35
13.23
1 4 .6 3
18.32
17.05
15.27
13.96
1 6 .1 5
17.91
14.12
13.69
14.47
1 5 .0 5
16.01
15.24
14.26
14.58
1 5 .0 2
15.85
16.13
14.28
14.54
1 5 .2 0
18.23
20.12
15.33 19.45
14.68 16.14
15.49 16.60
1 5 .9 3 1 8 .0 8
21.17
19.41
19.46
18.32
1 9 .5 9
18.52
18.96
20.24
19.23
1 9 .2 4
19.45
20.08
17.71
18.51
1 8 .9 4
18.39
17.21
16.81
16.76
1 7 .2 9
19.29
17.83
17.23
16.41
1 7 .6 9
18.53
15.12
16.72
16.89
1 6 .8 1
18.69
16.53
17.36
15.99
1 7 .1 4
18.84
16.51
16.59
15.65
1 6 .9 0
18.59
14.60
15.98
14.62
1 5 .9 5
17.62
14.68
16.18
15.30
1 5 .9 4
16.44
14.48
14.14
15.27
1 5 .0 8
16.15
12.94
14.29
15.48
1 4 .7 2
15.21
13.64
14.29
14.75
1 4 .4 7
15.50
15.37
13.42
14.02
1 4 .5 8
15.94
13.52
15.32
15.22
1 5 .0 0
16.36
13.59
14.18
14.09
1 4 .5 6
16.01
14.15
14.92
14.94
1 5 .0 0
16.00
14.11
14.52
14.85
1 4 .8 7
18.97
21.66
15.99 18.42
17.26 17.93
15.91 17.63
1 7 .0 3 1 8 .9 1
18.81
19.72
18.76
18.78
1 9 .0 2
19.91
19.11
17.76
17.42
1 8 .5 5
18.13
18.31
17.13
17.61
1 7 .7 9
19.04
16.79
16.98
16.20
1 7 .2 5
19.21
15.17
17.38
16.28
1 7 .0 1
18.16
16.35
17.05
16.49
1 7 .0 1
18.42
16.12
16.12
16.24
1 6 .7 3
18.84
16.11
16.36
16.05
1 6 .8 4
17.44
15.08
15.28
15.33
1 5 .7 8
17.66
15.22
14.46
15.22
1 5 .6 4
15.27
13.11
14.82
13.76
1 4 .2 4
15.52
14.03
13.04
14.49
1 4 .2 7
14.25
12.15
13.77
13.48
1 3 .4 1
15.72 15.34
14.09
12.66
13.50 13.55
12.84 13.02
1 4 .0 4 1 3 .6 4
15.08
14.14
12.63
14.25
1 4 .0 2
14.87
13.67
12.91
13.09
1 3 .6 4
17.36
13.95
14.19
14.87
1 5 .0 9
18.36
17.87
15.30
15.80
1 6 .8 3
18.82
16.27
16.93
17.77
1 7 .4 5
17.86
17.62
16.97
18.62
1 7 .7 6
18.01
17.66
16.20
17.84
1 7 .4 3
SD
1 .3 4 0 .44 0 .97 0 .73 1 .5 2 1 .2 2 1 .6 8 1 .3 9 0 .93 1 .0 8 1 .9 2 1 .9 4 0 .78 0 .93 1 .5 7 2 .00 1 .1 8 0 .73 1 .0 4 0 .76 1 .2 2 1 .3 9 1 .1 8 1 .3 6 1 .8 8 1 .2 7 1 .0 2 1 .4 1 0 .67 1 .0 2 1 .0 4 1 .2 3 0 .76 0 .8 1 1 .4 3 1 .8 6 0 .47 1 .1 6 0 .53 1 .2 4 1 .7 2 0 .82 1 .1 3 1 .3 4 1 .1 1 1 .3 9 0 .98 1 .0 3 0 .90 1 .2 3 1 .1 9 1 .0 2 0 .89 1 .5 6 1 .5 1 1 .1 0 0 .68 0 .83
Page A51 of A172
Appendix to TNO-report of 3M-02_____________ Final version
Time group 1 vs 3 (hr) n n p 6.1 4 4 0.886 7.4 4 4 0.343 8.6 4 4 0.343 9.9 4 4 0.886 11.1 4 4 0.343 12.4 4 4 0.686 13.6 4 4 0.343 14.9 4 4 0.343 16.1 4 4 0.200 17.4 4 4 1.000 18.6 4 4 0.886 19.9 4 4 0.343 21.1 4 4 0.200 22.4 4 4 0.343 23.6 4 4 1.000 24.9 4 4 0.886 26.1 4 4 0.114 27.4 4 4 0.686 28.6 4 4 0.486 29.9 4 4 0.029 31.1 4 4 0.686 32.4 4 4 0.886 33.6 4 4 0.886 34.9 4 4 1.000 36.1 4 4 0.486 37.4 4 4 0.686 38.6 4 4 1.000 39.9 4 4 0.886 41.1 4 4 0.886 42.4 4 4 0.886 43.6 4 4 0.486 44.9 4 4 0.686 46.1 4 4 0.486 47.4 4 4 0.886 48.6 4 4 0.886 49.9 4 4 0.057 51.1 4 4 0.343 52.4 4 4 0.686 53.6 4 4 0.886 54.9 4 4 0.886 56.1 4 4 0.686 57.4 4 4 1.000 58.6 4 4 0.200 59.9 4 4 0.886 61.1 4 4 0.886 62.4 4 4 0.486 63.6 4 4 0.886 64.9 4 4 0.886 66.1 4 4 0.686 67.4 4 4 1.000 68.6 4 4 0.686 69.9 4 4 0.343 71.1 4 4 0.886 72.4 4 4 0.886 73.6 4 4 0.886 74.9 4 4 0.886 76.1 4 4 0.886 77.4 4 4 0.886
gr
n 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
aEnergy expenditure p-values Mann Whitney U-test group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6 p n p nn p
0.057
0.057 4 0.029 4 0.029
0.200
0.200
0.057 4 0.200 4 0.029
0.114
0.114
0.114 4 0.343 4 0.114
0.686
0.886
0.200 4 0.486 4 0.057
0.200
0.114
0.114 4 0.886 4 0.343
0.486
0.057
0.114 4 0.200 4 0.200
0.343
0.200
0.057 4 0.686 4 0.343
0.343
0.029
0.029 4 0.114 4 0.057
0.343
0.029
0.029 4 0.686 4 0.686
0.886
0.114
0.200 4 0.486 4 0.686
0.686
0.343
0.114 4 0.343 4 0.200
0.343
0.343
0.886 4 0.114 4 0.200
0.686
0.114
0.057 4 0.486 4 0.343
0.686
0.029
0.029 4 0.114 4 0.114
0.686
0.029
0.029 4 0.686 4 0.686
0.886
0.886
1.000 4 0.686 4 0.686
0.486
0.029
0.057 4 0.029 4 0.486
0.343
0.029
0.029 4 0.029 4 0.029
0.343
0.114
0.029 4 0.114 4 0.029
0.486
0.114
0.029 4 0.886 4 0.114
0.686
0.114
0.057 4 0.200 4 0.114
0.343
0.886
0.200 4 0.486 4 0.200
0.686
0.200
0.114 4 0.200 4 0.057
0.343
0.686
0.057 4 0.686 4 0.200
0.200
0.057
0.114 4 0.486 4 0.486
0.886
0.200
0.114 4 0.686 4 0.486
0.886
0.200
0.057 4 0.486 4 0.200
0.486
0.200
0.200 4 0.486 4 0.343
0.686
0.200
0.057 4 0.486 4 0.200
1.000
0.686
0.200 4 0.686 4 0.686
0.343
0.029
0.029 4 1.000 4 0.343
0.114
0.200
0.200 4 0.686 4 0.343
0.886
0.057
0.200 4 0.686 4 0.200
0.486
0.686
0.114 4 0.114 4 0.886
1.000
0.486
0.486 4 0.886 4 0.886
0.886
0.057
0.114 4 0.486 4 0.886
0.686
0.029
0.029 4 0.200 4 0.029
0.486
0.057
0.029 4 0.029 4 0.029
0.886
0.029
0.029 4 0.114 4 0.029
0.886
0.343
0.057 4 0.486 4 0.200
0.486
0.114
0.114 4 0.343 4 0.343
0.486
0.686
0.029 4 0.686 4 0.057
0.200
0.200
0.029 4 0.686 4 0.200
0.200
0.686
0.029 4 0.686 4 0.200
0.210
0.686
0.029 4 0.886 4 0.029
0.200
0.114
0.029 4 0.114 4 0.029
0.114
0.686
0.114 4 0.486 4 0.200
0.686
0.343
0.200 4 0.486 4 0.486
0.486
0.200
0.057 4 0.200 4 0.114
0.886
0.686
0.200 4 0.686 4 0.486
0.343
0.057
0.057 4 0.486 4 0.200
0.486
0.029
0.114 4 0.114 4 0.057
0.686
0.343
0.486 4 0.686 4 0.886
1.000
0.686
0.343 4 0.686 4 0.686
0.486
0.686
0.686 4 0.686 4 0.686
0.114
0.029
0.200 4 0.029 4 0.200
0.200
0.200
0.029 4 0.343 4 0.029
0.486
0.200
0.114 4 0.343 4 0.114
0.686
04-12-2006 p<0.05
Page A52 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Appendix E. Fecal bile acid, sterol and fatty acid measurements
E1. Fecal weight measurements, bodyweight and food intake measurements
Bodyweight and food intake during collecting feces
Group# Mouse# cage# 11 21 32 42 53 63 74 85 95 10 5
2 11 6 2 12 6 2 13 6 2 14 7 2 15 8 2 16 8 2 17 8 2 18 9 2 19 9 2 20 10 3 21 11 3 22 12 3 23 13 3 24 13 3 25 14 3 26 15 3 27 15 3 28 15
13 29 16
3 30 17 31 18 32 18 33 19 34 19 35 19 36 19 37 20 38 20 39 21 40 21
5 41 22 5 42 22 5 43 22 5 44 22 5 45 23 5 46 24 5 47 25 5 48 25 5 49 26 5 50 27 6 51 28 6 52 29 6 53 29 6 54 30 6 55 30 6 56 31 6 57 31 6 58 31 6 59 31 6 60 32 7 61 33 7 62 34 7 63 35 7 64 36 7 65 36 7 66 37 7 67 38 7 68 38 7 69 39 7 70 39
collection point 1;2daysfecescollection
eartag startcollectionpoint 1
endcollection point 1
Average Foodintake(g)
L R bodyweight (g) foodoncage(g) bodyweight (g) foodoffcage(g) bodyweight(g) coll. point 1
0 1 33.9 33.9 33.90
1 2 31.2
49.7
30.5
38.9 30.85
10.8
0 0 30.4 31.0 30.70
2 0 28.1
51.9
28.2
39.5 28.15
12.4
0 0 31.1 30.6 30.85
0 2 29.5
47.4
29.4
35.8 29.45
11.6
1 1 26.7
26.9
27.2
20.5 26.95
6.4
0 0 30.4 30.6 30.50
0 2 30.6 31.0 30.80
1 2 27.2
61.6
27.3
44.8 27.25
16.8
1 0 33.0 33.1 33.05
2 1 29.8 30.0 29.90
0 2 31.2
68.5
32.0
48.6 31.60
19.9
0 1 32.8
31.7
33.1
24.8 32.95
6.9
1 0 30.8 31.0 30.90
0 1 30.1 29.8 29.95
2 0 32.8
68.0
32.8
47.2 32.80 20.8
0 0 26.5 26.6 26.55
2 1 26.9
42.3
26.6
31.8 26.75
10.5
1 0 29.0
29.2
28.8
23.4 28.90
5.8
0 1 27.8
32.1
28.3
26.2 28.05
5.9
0 2 28.8
32.9
28.9
27.2 28.85
5.7
0 1 32.2 32.3 32.25
1 1 28.8
44.9
28.9
30.3 28.85
14.6
1 0 25.8
31.2
26.3
25.2 26.05
6.0
0 1 28.7 28.5 28.60
2 0 30.1 30.1 30.10
1 2 25.0
77.5
24.7
61.0 24.85
16.5
0 2 29.8
23.1
29.7
17.1 29.75
6.0
1 0 27.0
26.3
27.0
20.0 27.00
6.3
1 0 30.3 30.2 30.25
0 2 29.3
48.3
29.2
36.3 29.25
12.0
0 0 29.2 28.9 29.05
1 0 30.9 30.7 30.80
0 1 31.0 30.7 30.85
0 2 25.6
90.1
25.8
68.4 25.70 21.7
0 0 27.0 26.7 26.85
1 1 30.2
43.1
30.2
31.0 30.20 12.1
0 0 27.7 27.2 27.45
1 1 27.7
47.9
27.7
36.0 27.70
11.9
1 0 28.9 30.0 29.45
0 1 25.2 25.3 25.25
1 1 27.6 27.8 27.70
1 2 28.5
90.3
28.1
68.8 28.30 21.5
1 2 28.6
37.8
29.7
30.8 29.15
7.0
2 0 28.5
27.2
28.6
21.1
28.55
6.1
0 1 30.3 30.1 30.20
1 1 29.3
50.7
29.9
37.2 29.60
13.5
0 0 27.3
27.7
27.2
22.1
27.25
5.6
1 2 27.7
27.1
28.7
20.2 28.20
6.9
0 1 28.4
31.9
28.5
26.2 28.45
5.7
1 0 31.3 31.5 31.40
1 2 27.3
52.3
27.2
40.4 27.25
11.9
1 0 30.2 30.0 30.10
0 2 28.8
42.9
28.4
31.2 28.60
11.7
0 0 25.6 25.4 25.50
0 1 27.6 27.8 27.70
0 2 29.1 29.1 29.10
2 2 30.3
88.0
30.7
67.7 30.50 20.3
2 1 26.7
30.5
26.7
25.0 26.70
5.5
2 0 28.2
29.1
27.7
23.4 27.95
5.7
1 0 27.3
31.2
26.7
25.5 27.00
5.7
0 2 26.2
34.7
26.6
29.3 26.40
5.4
0 0 28.2 28.5 28.35
1 2 27.4
21.2
28.2
9.4 27.80 11.8
0 0 26.1
26.4
26.1
21.3 26.10
5.1
0 0 26.0 26.2 26.10
2 0 26.7
53.0
26.9
42.5 26.80
10.5
1 0 23.6 23.0 23.30
0 1 25.1
43.8
24.6
32.4 24.85
11.4
Page A53 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
G roup# M ouse#
cage#
11 21 32 42 53 63 74 85 95 10 5 2 11 6 2 12 6 2 13 6 2 14 7 2 15 8 2 16 8 2 17 8 2 18 9 2 19 9 2 20 10 3 21 11 3 22 12 3 23 13 3 24 13 3 25 14 3 26 15 3 27 15 3 28 15
13 29 16
3 30 17 31 18 32 18 33 19 34 19 35 19 36 19 37 20 38 20 39 21 40 21
5 41 2 2 5 42 22 5 43 22 5 44 22 5 45 23 5 46 24 5 47 25 5 48 25 5 49 26 5 50 27 6 51 2 8 6 52 29 6 53 29 6 54 30 6 55 30 6 56 31 6 57 31 6 58 31 6 59 31 6 60 32 7 61 3 3 7 62 34 7 63 35 7 64 36 7 65 36 7 66 37 7 67 38 7 68 38 7 69 39 7 70 39
e a rta g LR 01 12 00 20 00 02 11 00 02 12 10 21 02 01 10 01 20 00 21 10 01 02 01 11 10 01 20 12 02 10 10 02 00 10 01 02 00 11 00 11 10 01 11 12 12 20 01 11 00 12 01 10 12 10 02 00 01 02 22 21 20 10 02 00 12 00 00 20 10 01
co lle c tio n p oint 2; 3 days fe c e s co lle ctio n
sta rt c o lle ctio n p oint 2
end c o lle ctio n point 2
bod yw e ig h t (g) fo o d on ca g e (g) bod yw e ig h t (g) fo o d o ff ca g e (g)
3 3 .9 3 0 .5
3 8 .9
3 2 .9 30.1
22.1
31 30
2 8 .2
3 9 .5
2 8 .3
19.5
3 0 .6
2 9 .2
2 9 .4
3 5 .8
3 0 .4
16.8
2 7 .2 3 0 .6
2 0 .5
2 6 .3 30
10.7
31 2 7 .3
4 4 .8
3 0 .5 27
19.2
33.1
3 2 .2
30 30 32 48.6 31.2 19.8
33.1
2 4 .8
3 3 .6
1 2 .4
31 2 9 .8
30.1 30
3 2 .8
4 7 .2
3 2 .5
14.2
2 6 .6
2 6 .3
2 6 .6 2 8 .8
3 1 .8 2 3 .4
2 6 .9 29
13.6 1 3 .4
2 8 .3
2 6 .2
2 8 .4
15.7
2 8 .9
2 7 .2
2 9 .2
16.2
3 2 .3 2 8 .9
3 0 .3
3 2 .3 2 9 .2
7.1
2 6 .3 2 8 .5
2 5 .2
26.1 2 8 .8
15.6
30.1
2 9 .8
2 4 .7 2 9 .7
6 1 .0 17.1
25.1 2 9 .4
3 2 .7 8.0
27 20.0 27.6
9.4
3 0 .2
2 9 .6
2 9 .2
3 6 .3
2 8 .7
16.5
2 8 .9 3 0 .7
2 9 .6 3 0 .5
3 0 .7
3 0 .7
2 5 .8
6 8 .4
2 6 .2
3 3 .2
2 6 .7
2 6 .9
3 0 .2
3 1 .0
30.1
11.1
2 7 .2 2 7 .7
3 6 .0
2 7 .2 2 7 .7
17.7
30 2 5 .3
29.1 2 5 .5
2 7 .8
2 8 .6
28.1 2 9 .7
6 8 .8 3 0 .8
2 8 .8 30.1
3 2 .3 20.1
2 8 .6
21.1
2 8 .6
10.6
30.1 2 9 .9
3 7 .2
30.1 2 9 .7
15.5
2 7 .2 2 8 .7
22.1 2 0 .2
27 2 8 .4
12.5 10.2
2 8 .5
2 6 .2
2 8 .7
16.6
3 1 .5 2 7 .2
4 0 .4
3 1 .4 27
2 0 .3
30 29.7
2 8 .4
3 1 .2
28
12.3
2 5 .4
2 5 .3
2 7 .8 29.1
2 6 .9 2 8 .8
3 0 .7
6 7 .7
3 0 .9
3 6 .7
2 6 .7
2 5 .0
2 6 .3
16.0
2 7 .7
2 3 .4
29.1
12.7
2 6 .7
2 5 .5
2 7 .8
16.3
2 6 .6 2 8 .5
2 9 .3
2 6 .8 2 8 .9
2 0 .0
2 8 .2 26.1
56.1 2 1 .3
2 7 .6 2 6 .2
3 8 .8 12.1
2 6 .2
2 6 .7
2 6 .9 23
2 4 .6
4 2 .5 3 2 .4
2 7 .2 2 3 .6 2 5 .7
2 4 .9 15.0
A verage b o d yw e ig h t (g)
3 3 .4 0 3 0 .3 0 3 0 .5 0 2 8 .2 5 2 9 .9 0 2 9 .9 0 2 6 .7 5 3 0 .3 0 3 0 .7 5 2 7 .1 5 3 2 .6 5 3 0 .0 0 3 1 .6 0 3 3 .3 5 3 0 .5 5 2 9 .9 0 3 2 .6 5 2 6 .4 5 2 6 .7 5 2 8 .9 0 2 8 .3 5 2 9 .0 5 3 2 .3 0 2 9 .0 5 2 6 .2 0 2 8 .6 5 2 9 .9 5 2 4 .9 0 2 9 .5 5 2 7 .3 0 2 9 .9 0 2 8 .9 5 2 9 .2 5 3 0 .6 0 3 0 .7 0 2 6 .0 0 2 6 .8 0 3 0 .1 5 2 7 .2 0 2 7 .7 0 2 9 .5 5 2 5 .4 0 2 8 .2 0 2 8 .4 5 2 9 .9 0 2 8 .6 0 3 0 .1 0 2 9 .8 0 2 7 .1 0 2 8 .5 5 2 8 .6 0 3 1 .4 5 2 7 .1 0 2 9 .8 5 2 8 .2 0 2 5 .3 5 2 7 .3 5 2 8 .9 5 3 0 .8 0 2 6 .5 0 2 8 .4 0 2 7 .2 5 2 6 .7 0 2 8 .7 0 2 7 .9 0 2 6 .1 5 2 6 .4 5 2 7 .0 5 2 3 .3 0 2 5 .1 5
F oo d in take (g) coll. p o in t 2
1 6 .8 2 0 .0 1 9 .0 9.8
2 5 .6
2 8 .8 1 2 .4
3 3 .0 1 8 .2 10.0 1 0 .5 1 1 .0 2 3 .2 9.6
2 8 .3 9.1 10.6
1 9 .8
3 5 .2 1 9 .9
18.3
3 6 .5 1 0 .7 1 0 .5 2 1 .7 9.6 10.0 9.6 20.1 1 8 .9
3 1 .0 9.0 1 0 .7 9.2 9.3 1 7 .3 9.2 1 7 .6
1 7 .4
Page A54 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
G roup# M ouse#
cage#
111
12
1
13 2
14 2
15 3
16 3
17 4
18 5
19 5
1 10 5
2 11 6
2 12 6
2 13 6
2 14 7
2 15 8
2 16 8
2 17 8
2 18 9
2 19 9
2 20 10
3 21 11
3 22 12
3 23 13
3 24 13
3 25 14
3 26 15
3 27 15
3 28 15
13 29 16
3 30 17 31 18 32 18 33 19 34 19 35 19 36 19 37 20 38 20
3 9 21
4 0 21
5 41 22
5 42 22
5 43 22
5 44 22
5 45 23
5 46 24
5 47 25
5 48 25
5 49 26
5 50 27
6 51 28 6 52 29 6 53 29
6 54 30
6 55 30
6 5 6 31
6 57 31
6 5 8 31
6 5 9 31
6 60 32
7 61 33
7 62 34
7 63 35
7 64 36
7 65 36
7 66 37
7 67 38
7 68 38
7 69 39
7 70 39
eartag LR 01 12 00 20 00 02 11 00 02 12 10 21 02 01 10 01 20 00 21 10 01 02 01 11 10 01 20 12 02 10 10 02 00 10 01 02 00 11 00 11 10 01 11 12 12 20 01 11 00 12 01 10 12 10 02 00 01 02 22 21 20 10 02 00 12 00 00 20 10 01
collection point 3; 2 da ys fe c e s collection
start collection point 3
end collection point 3
bod yw eight (g) fo o d on cage (g) bodyw eight (g) food o ff c a g e (g)
32.9
33.2
30.1
78.3
30.2
68.3
30 30.2
28.3
73.4
28.4
63.2
29.2
31.1
30.4
67.0
29.6
56.6
26.3
38.3
26.5
33.4
30 30.8
30.5 27
94.8
30.7 27.1
81.0
32.2 30
32.8 30.0
31.2
97.1
31.8
80.4
33.6 30.1
42.0
33.1 30.3
36.8
30 30.9
32.5 26.3
85.6
32.7 25.9
67.2
26.9 29
60.9 46.3
26.5 29.1
52.8 41.2
28.4
39.9
29.1
34.3
29.2
40.9
29.2
35.5
32.3 29.2
56.5
32.5 29.3
43.8
26.1
46.1
26.4
41.0
28.8 29.8
29.1 30.2
25.1
1 1 4 .5
25.4
1 0 0 .9
29.4 27.6
34.9 37.9
29.6 27.8
29.9 33.3
29.6
29.9
28.7 29.6
61.5
29.2 29.8
50.2
30.5
30.5
30.7 26.2
1 3 0 .6
31.1 26.5
1 1 1 .9
26.9
28.0
30.1 27.2
70.0
30.9 27.5
58.0
27.7
67.9
27.5
57.3
29.1 25.5
29.8 25.6
28.6
29.2
28.8 30.1
1 3 8 .4 52.3
29.1 29.6
1 1 9 .7 47.7
28.6
37.3
28.6
31.9
30.1 29.7
74.9
30.6 30.2
52.3
27 28.4
39.9 35.4
27.3 28.6
35.1 30.3
28.7
44.3
28.7
38.8
31.4
32.1
27 66.8 27.4 55.8
29.7
30.2
28 60.0 28.5 49.9
25.3
25.6
26.9
27.2
28.8
28.6
30.9 26.3
1 4 2 .0 46.7
30.6 26.6
1 2 4 .9 41.8
29.1
35.7
28.5
30.7
27.8
42.4
27.8
37.5
26.8
46.1
27.0
40.3
28.9
28.7
27.6
90.6
28.2
81.1
26.2
39.7
27.1
34.2
26.7
26.7
27.2
76.5
27.3
66.5
23.6
23.8
25.7
65.9
25.4
56.1
A v e ra g e bodyw eight (g)
3 3 .0 5 3 0 .1 5 3 0 .1 0 2 8 .3 5 3 0 .1 5 3 0 .0 0 2 6 .4 0 3 0 .4 0 3 0 .6 0 2 7 .0 5 3 2 .5 0 3 0 .0 0 3 1 .5 0 3 3 .3 5 3 0 .2 0 3 0 .4 5 3 2 .6 0 2 6 .1 0 2 6 .7 0 2 9 .0 5 2 8 .7 5 2 9 .2 0 3 2 .4 0 2 9 .2 5 2 6 .2 5 2 8 .9 5 3 0 .0 0 2 5 .2 5 2 9 .5 0 2 7 .7 0 2 9 .7 5 2 8 .9 5 2 9 .7 0 3 0 .5 0 3 0 .9 0 2 6 .3 5 2 7 .4 5 3 0 .5 0 2 7 .3 5 2 7 .6 0 2 9 .4 5 2 5 .5 5 2 8 .9 0 2 8 .9 5 2 9 .8 5 2 8 .6 0 3 0 .3 5 2 9 .9 5 2 7 .1 5 2 8 .5 0 2 8 .7 0 3 1 .7 5 2 7 .2 0 2 9 .9 5 2 8 .2 5 2 5 .4 5 2 7 .0 5 2 8 .7 0 3 0 .7 5 2 6 .4 5 2 8 .8 0 2 7 .8 0 2 6 .9 0 2 8 .8 0 2 7 .9 0 2 6 .6 5 2 6 .7 0 2 7 .2 5 2 3 .7 0 2 5 .5 5
Food in take (g) coll. po in t 3
10.0 10.2 10.4 4.9
13.8
16.7 5.2
18.4 8.1 5.1 5.6 5.4 12.7 5.1
13.6 5.0 4.6
11.3
18.7 12.0
10.6
18.7 4.6 5.4 2 2 .6 4.8 5.1 5.5 11.0 10.1
17.1 4.9 5.0 4.9 5.8 9.5 5.5 10.0
9.8
Page A55 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Fecal weight
Group
1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 5 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 7 7
cage#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Feces collection point 1; 2 days collection
number of Tube empty Tube full T ube full after freeze drying
mice (g) (g)
(g)
2 6.6630 8.3248
8.0444
2 6.6490 8.6605
8.3426
2 6.6221 8.6309
8.3229
1
6.6808
7.664
7.4697
3 6.6712 9.4474
8.9658
3 6.6634 9.8825
9.4253
1 6.6794 7.8244
7.6266
3 6.6207 10.2937
9.7316
2 6.6605 8.2584 1 6.6630 7.5596
8.0008 7.4162
1 6.6697 7.5244
7.3942
1 6.6594 7.5056
7.3806
2 6.6379 9.0681
8.6519
1 6.6624 7.5339
7.4049
3
6.6568
9.433
8.9562
1 6.6607 7.5433
7.3604
1 6.6312 7.6683
7.5344
2 6.6765 8.6953
8.3655
4 6.6311 10.3697
9.7743
2 6.6493 8.6134
8.2667
2 6.6603 8.6425
8.2974
4 6.6918 10.4134
9.8868
1 6.6683 7.7517 1 6.7028 7.6752
7.5567 7.5296
2 6.6868 9.2271 1 6.6825 7.5842
8.8008 7.456
1 6.6889 7.8388
7.6669
1 6.6648 7.6251 2 6.6617 8.6518
7.4945 8.3356
2 6.6503 8.6434
8.3383
4 6.6110 9.8507
9.3193
1 6.6541 7.4635
7.3215
1 6.6615 7.3485
7.2406
1 6.6589 7.5622
7.3939
1
6.6820
7.476
7.3376
2 6.6335 8.4094 1 6.6727 7.4706
8.0542 7.3318
2 6.6890 8.3375 2 6.6706 8.2396
8.054 7.9761
freeze dried feces (g) 1.3814 1.6936 1.7008 0.7889 2.2946 2.7619 0.9472 3.1109 1.3403 0.7532 0.7245 0.7212 2.0140 0.7425 2.2994 0.6997 0.9032 1.6890 3.1432 1.6174 1.6371 3.1950 0.8884 0.8268 2.1140 0.7735 0.9780 0.8297 1.6739 1.6880 2.7083 0.6674 0.5791 0.7350 0.6556 1.4207 0.6591 1.3650 1.3055
Page A56 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group
1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 5 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 7 7
cage#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Feces collection point 2; 3 days collection
number of Tube empty Tube full T ube full after freeze drying
mice 2
(g) 6.6728
(g) 9.043
(g) 8.654
2
6.6313
9.7853
9.3421
2
6.6987
9.5813
9.188
1
6.6499
8.1391
3
6.6805
10.6433
7.9395 10.0911
3 6.6610 11.3687
10.736
1
6.6450
8.426
8.1553
3 6.6479 12.3927
11.5086
2
6.6676
9.2172
8.8482
1
6.6137
8.0501
7.8405
1
6.6564
8.1514
7.945
1
6.6806
8.3368
8.1005
2 6.6554 10.2976
9.7616
1
6.6905
8.1358
7.9299
3 6.6709 11.0804
10.4427
1
6.6500
8.0103
7.7806
1
6.6622
8.2678
8.0649
2
6.6790
9.8571
9.3923
4 6.6452 12.0852
11.2859
2
6.6370
9.933
2
6.6464
9.514
9.4414 9.0495
4 6.6068 12.3762
11.578
1
6.6411
8.2187
7.9954
1
6.6557
8.1263
7.9237
2 6.6822 10.3758
9.8975
1
6.6134
8.0183
7.792
1
6.6799
8.1596
7.914
1
6.6762
8.1471
7.9636
2
6.6989
9.824
9.3799
2
6.6732
9.5294
9.1304
4
6.6621
11.3048
1
6.6310
7.9252
10.6299 7.7404
1
6.6902
8.1846
7.96
1
6.6712
7.9152
7.7309
1
6.6337
7.9533
7.7519
2
6.6633
9.2718
8.8526
1
6.6324
7.8893
7.7077
2
6.6776
9.1802
8.8063
2
6.6697
9.253
8.8469
freeze dried feces (g)
1.9812 2.7108 2.4893 1.2896 3.4106
4.0750 1.5103 4.8607 2.1806 1.2268
1.2886 1.4199 3.1062 1.2394 3.7718 1.1306 1.4027 2.7133 4.6407 2.8044 2.4031
4.9712 1.3543 1.2680 3.2153 1.1786 1.2341
1.2874 2.6810 2.4572 3.9678 1.1094
1.2698 1.0597 1.1182 2.1893 1.0753 2.1287 2.1772
Page A57 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Feces collection point 3; 2 days collection
Group cage# number of Tube empty Tube full Tube full after freeze drying freeze dried
1
1
mice 2
6.6(g4)29 8.5(g8)21
8.2(g3)99
feces (g) 1.5970
1 2 2 6.6725 8.9822
8.6509
1.9784
1 3 2 6.6831 8.9939
8.653
1.9699
1 4 1 6.6645 7.7309
7.5727
0.9082
1 5 3 6.6432 9.9317
9.4021
2.7589
2 6 3 6.6844 10.106
9.6123
2.9279
2 7 1 6.6652 7.8515
7.6749
1.0097
2 8 3 6.6453 10.6479
10.0341
3.3888
2 9 2 6.6397 8.3585
8.1165
1.4768
2 10 1 6.6569 7.7971
7.6376
0.9807
3 11 1 6.6860 7.7179
7.5628
0.8768
3 12 1 6.6590 7.7398
7.58
0.9210
3 13 2 6.6605 9.3189
8.8877
2.2272
3 14 1 6.6334 7.6444
7.4947
0.8613
3 15 3 6.6582 9.6295
9.1847
2.5265
33 1167 11 66..66164952 77..67385914
77..4683119
00..89667148
4 18 2 6.6935 9.0966
8.7345
2.0410
4 19 4 6.6306 10.6276
10.077
3.4464
4 20 2 6.6585 9.1542
8.7793
2.1208
4 21 2 6.6901 8.8739
8.5242
1.8341
5 22 4 6.6840 10.9177
10.2953
3.6113
5 23 1 6.6687 7.6866
7.5361
0.8674
5 24 1 6.6635 7.8102
7.6563
0.9928
5 25 2 6.6833 9.43
9.0427
2.3594
5 26 1 6.6333 7.6942
7.5479
0.9146
5 27 1 6.6752 7.7479
7.5966
0.9214
6 28 1 6.6351 7.7734
7.6365
1.0014
6 29 2 6.6633 9.0885
8.5695
1.9062
6 30 2 6.6611 8.8705
8.5556
1.8945
6 31 4 6.6657 10.3688
9.9106
3.2449
6 32 1 6.6786 7.7434
7.5484
0.8698
7 33 1 6.6860 7.6314
7.485
0.7990
7 34 1 6.6887 7.5625
7.393
0.7043
7 35 1 6.6783 7.9028
7.6867
1.0084
7 36 2 6.6551 8.561
8.2858
1.6307
7 37 1 6.6481 7.617
7.4543
0.8062
7 38 2 6.6522 8.6462
8.3568
1.7046
7 39 2 6.6641 8.7084
8.3702
1.7061
Page A58 of A172
Appendix to TNO-report of 3M-02
Final version
Feces weight, bodyweight and food intake GC samples
GC-1 Fecescollection3M-02week6; collectionpoint 1,2dayscollection
Group cage# fecescollected Bodyweight Foodintake
code (g) (g) (g)
1:1-1 1 1,2 3.075 123.6 23.2
1:1-2 1
3,4 2.4897 87.25
18
1:1-3 1
5 2.2946 88.55 16.8
1:2-4 2
6 2.7619 94.55 19.9
1:2-5 2
7,8 4.0581 126.6 27.7
1:2-6 2 9,10 2.0935 82.2
16.3
1:3-7 3 11,12,13 3.4597 118 26.2
1:3-8 3
15 2.2994 83.55 16.5
1:3-9 3 14,16,17 2.3454 82.8
18.3
1:4-10 4
18 1.689 59.5
12
1:4-11 1:4-12
t
44
19 3.1432 20,21 3.2545
116.4 112.2
21.7 24
1:5-13 5 22 3.195 110.7 21.5
1:5-14 5 24,25 2.9408 88.35
19.6
1:5-15 5 23,26,27 2.6399
84.6
19.5
1:6-16 6
28,29 2.5036
87.1
17.6
1:6-17 6
31 2.7083 112.8 20.3
1:6-18 6
30,32 2.3554
85.4
17.2
1:7-19 7 33,34,35 1.9697 81.35
16.8
1:7-20 7 36,37 2.0798 82.25
16.9
1:7-21 7 38,39 2.6705 101.05 21.9
GC-2 code 2:1-1 2:1-2 2:1-3 2:2-4 2:2-5 2:2-6 2:3-7 2:3-8 2:3-9 2:4-10 2:4-11 2:4-12 2:5-13 2:5-14 2:5-15 2:6-16 2:6-17 2:6-18 2:7-19 2:7-20 2:7-21
Fecescollection3M-02week6; collectionpoint2, 3dayscollection |
Group cage# fecescollected Bodyweight Foodintake
(g) (g) (g) 1 1,2 4.692 122.45 36.8
1 3,4 3.7789 86.55 28.8
1
5
3.4106
88.2
25.6
2 6 4.075 94.25 28.8
2 7,8 6.371 126.45 45.4
2 9,10 3.4074 82.1 28.2
3 11,12,13 5.8147 118.75 44.7
3 15 3.7718 83.5 28.3
3 14,16,17 3.7727 83.05 29.3
4 18 2.7133 58.85 19.8
44
19 20,21
4.6407 5.2075
116.55 111.85
35.2 38.2
5 22 4.9712 111.6 36.5
5 24,25 4.4833 88.5 32.2
5 23,26,27 3.767 85.55 30.3
6 28,29 3.9684 87.15 29.7
6 31 3.9678 112.45 31
6 30,32 3.5666 84.55 27.9
7 33,34,35 3.4477 82.35 29.2
7 36,37 3.2646 82.75 26.5
7 38,39 4.3059 101.95
35
GC-3 code 3:1-1 3:1-2 3:1-3 3:2-4 3:2-5 3:2-6 3:3-7 3:3-8 3:3-9 3:4-10 3:4-11 3:4-12 3:5-13 3:5-14 3:5-15 3:6-16 3:6-17 3:6-18 3:7-19 3:7-20 3:7-21
Fecescollection3M-02week6; collectionpoint3, 2dayscollection
Group cage# fecescollected Bodyweight Foodintake
(g) (g) 1 1,2 3.5754 121.65
(g) 20.2
1 3,4 2.8781 86.55
15.3
1 5 2.7589 88.05
13.8
2 6 2.9279 94
16.7
2 7,8 4.3985 126.6
23.6
2 9,10 2.4575 81.85
13.2
3 11,12,13 4.025
119.6
23.7
3 15 2.5265 84.2
13.6
3 14,16,17 2.6905 83.45
14.7
4 18 2.041
58.7
11.3
4 19 3.4464 117.45 18.7
4 20,21 3.9549
112.9
22.6
5 22 3.6113 112.85
18.7
5 24,25 3.3522
88.9
28
5 23,26,27 2.7034
85.5
14.5
6 28,29 2.9076 87.65
16.5
6 31
3.2449 111.95
17.1
6 30,32 2.7643 84.65
15
7 33,34,35 2.5117
83.5
15.7
7 36,37 2.4369 83.35
15
7 38,39 3.4107
103.2
19.8
04-12-2006
Page A59 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
E2. Fecal bile acid measurements
1:1-1____________________________
weight feces
5.8
mg
a-muricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
area
770 2880 837 1412 3635 3854 411
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1062.6 2880 979 1412 3925.8 5704 411
% |jg bile acid ng bile acid nmol bile acid/ mg feces mg feces
6.5
0.447
77.1
17.6
1.211
208.9
0.189 0.532
6.0
0.412
71.0
0.174
8.6
0.594
102.4
0.272
24.0
1.651
284.7
0.697
34.8
2.399
413.6
2.5
0.173
29.8
1.012 0.076
total I 13799 I
|
16375
| 100 | 7 | 1187 |
3.0~
nor-hyodeoxy | 23775 | 1 |
23775
| 23775
1:1-2____________________________
weight feces
6
mg
a-muricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
area
481 1551 1143 2203 2222 5717 261
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
663.78 1551 1337 2203 2399.76 8461 261
% jg bile acid ng bile acid nmol bile acid/ mg feces mg feces
3.9
0.276
46.0
0.113
9.2
0.645
107.4
0.274
7.9
0.556
92.6
13.1
0.916
152.6
0.227 0.405
14.2
0.997
166.2
0.407
50.1
3.517
586.1
1.5 0.108 18.1
1.435 0.046
total I 13578 I
|
16877
| 100 | 7 | 1169 |
2.9~
nor-hyodeoxy I 24059 | 1 |
24059
| 24059
1:1-3____________________________
weight feces
5.5
mg
a-muricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
area
304 1047 535 1334 1317 3031 192
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
419.52 1047 626 1334 1422.36 4486 192
% jg bile acid ng bile acid nmol bile acid/ mg feces mg feces
4.4
0.269
48.9
0.120
11.0
0.672
122.1
6.6 0.401 73.0
14.0
0.856
155.6
0.311 0.179 0.413
14.9
0.912
165.9
0.406
47.1
2.877
523.1
2.0
0.123
22.4
1.280 0.057
total I 7760 I
I 9527 | 100 | 6 | 1111 | 2.8~
nor-hyodeoxy | 15591 | 1 |
15591
| 15591
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A60 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2: 1-1___________________________
weight feces
7.2
mg
area response area corrected
% |jg bile acid ig bile acid nmol bile acid/
factor for response factor
mg feces mg feces
a-muricholate 314
1.38
433.32
13.0
0.940
130.5
0.319
deoxycholate 688
1
688
20.7
1.492
207.2
0.528
cholate
213
1
249
7.5
0.540
75.0
0.184
lithocholate
335
1
335
10.1
0.726
100.9
0.268
B-muricholate 803
1.08
867.24
26.1
1.880
261.2
0.639
w-muricholate hyodeo/urso
507
1 1
750
22.6
1.627
226.0
0.553
0
0.0 0.000 0.0
0.000
total | 2860 |
|
3323
| 100 | 7 | 1001 |
2.5
nor-hyodeoxy | 4612 | 1 |
I 2:1-2
weight feces
7.2
mg
4612
| 4612
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
1014 2554 2363 2730 4506 9183 213
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1399.32 2554 2765 2730
4866.48 13591 213
% jg bile acid ig bile acid nmol bile acid/
mg feces mg feces
5.0
0.472
65.5
0.160
9.1
0.861
119.6
0.305
9.8
0.932
129.4
0.317
9.7
0.920
127.8
0.339
17.3
1.640
227.8
48.3 4.581 636.3
0.558 1.557
0.8
0.072
10.0
0.025
total | 22563 |
|
28118
| 100 | 9 | 1316 |
3.3
nor-hyodeoxy | 29667 | 1 |
29667
| 29667
| 2:1-3 weight feces
5.1
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
1430 3201 3202 808 5434 7559 255
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1973.4 3201 3746 808 5868.72 11187 255
% jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
7.3
0.452
88.6
11.8
0.733
143.6
13.9
0.857
168.1
3.0
0.185
36.3
21.7
1.343
263.3
41.4
2.560
502.0
0.9
0.058
11.4
0.217 0.366 0.411 0.096 0.645 1.229 0.029
total I 21889 |
|
27040
| 100 | 6 | 1213 |
3.0
nor-hyodeoxy | 43696 | 1 |
43696
| 43696
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A61 of A172
Appendix to TNO-report of 3M-02
Final version
| 3:1-1 weight feces
7
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
755 6854 1031 1211 6883 6151 349
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1041.9 6854 1206 1211 7433.64 9103 349
%
3.8 25.2 4.4 4.5 27.3 33.5 1.3
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.319
45.6
0.112
2.101
300.1
0.764
0.370
52.8
0.129
0.371
53.0
0.141
2.278
325.5
0.797
2.790 0.107
398.6 15.3
0.976 0.039
total
| 23234 |
|
27199
| 100 | 8 | 1191 |
3.
nor-hyodeoxy | 32626 | 1 |
32626
| 32626
| 3:1-2 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.6
area
475 1558 748 1489 1894 3897 198
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
655.5 1558 875 1489 2045.52 5768 198
%
5.2 12.4 7.0 11.8 16.2 45.8 1.6
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.325 0.771 0.433 0.737 1.013 2.856 0.098
49.2 116.9 65.7 111.7 153.5 432.7 14.9
0.120 0.298 0.161 0.297 0.376 1.059 0.038
total
| 10259 |
|
12589
| 100 | 6 | 944 |
2~
nor-hyodeoxy | 20195 | 1 |
20195
| 20195
| 3:1-3 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.1
area
750 1906 1166 974 3492 4213 396
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1035 1906 1364 974 3771.36 6235 396
%
6.6 12.2 8.7 6.2 24.0 39.8 2.5
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.429 0.791 0.566 0.404 1.565 2.587 0.164
70.4 129.6 92.8 66.2 256.5 424.1 26.9
0.172 0.330 0.227 0.176 0.628 1.038 0.069
total
| 12897 |
|
15682
| 100 | 7 | 1067 |
2.6
nor-hyodeoxy | 24103 | 1 |
24103
| 24103
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A62 of A172
Appendix to TNO-report of 3M-02
Final version
1:2-4 weight feces
6.3
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
133 741 488 509 758 1756 133
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
183.54 741 571 509
818.64 2599 133
%
3.3 13.3 10.3 9.2 14.7 46.8 2.4
|jg bile acid ng bile acid nmol bile acid/ mg feces mg feces
0.211 0.853
33.5 135.4
0.082 0.345
0.657
104.3
0.255
0.586
93.0
0.247
0.942 2.991 0.153
149.6 474.8 24.3
0.366 1.162 0.062
total
I 4518 I
I
5555
| 100 | 6 | 1015 |
25
nor-hyodeoxy
8688
1:2-5 weight feces
6.4
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
385 1785 1437 1980 5071 4256 151
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
531.3 1785 1681 1980 5476.68 6299 151
%
3.0 10.0 9.4 11.1 30.6 35.2 0.8
|jg bile acid ng bile acid nmol bile acid/ mg feces mg feces
0.208 0.699 0.659 0.776 2.146 2.468 0.059
32.5 109.3 102.9 121.2 335.3 385.6
9.2
0.080 0.278 0.252 0.322 0.821 0.944 0.024
total
I 15065 I
I
17904
| 100 | 7 | 1096 |
2T
nor-hyodeoxy | 25522 | 1 |
25522
| 25522
1:2-6 weight feces
5.3
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
696 3055 1508 3344 2771 7511 363
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
960.48 3055 1764 3344 2992.68 11116 363
%
4.1 12.9 7.5 14.2 12.7 47.1 1.5
|jg bile acid ng bile acid nmol bile acid/ mg feces mg feces
0.324 1.032 0.596 1.130 1.011 3.756 0.123
61.2 194.7 112.5 213.2 190.8 708.6 23.1
0.150 0.496 0.275 0.566 0.467 1.734 0.059
total
I 19248 I
|
23596
| 100 |
8 | 1504 |
3T
nor-hyodeoxy | 29599 | 1 |
29599
| 29599
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A63 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:2-4 weight feces
7.7
mg
a-muricholate deoxycholate
cholate lithocholate
U-muricholate w-muricholate hyodeo/urso
area
564 1799 924 1298 1925 3892
response area corrected factor for response factor
% |jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
1.38 778.32
6.1
0.518
67.3
0.165
1
1799
14.1
1.198
155.6
0.396
1
1081
8.4
0.720
93.5
0.229
1
1298
10.1
0.864
112.2
0.298
1.08
2079
16.2
1.384
179.8
0.440
1
5760
45.0
3.836
498.1
1.219
1
0
0.0 0.000 0.0
0.000
total | 10402 |
|
12796
| 100 | 9 | 1107 |
2.7
nor-hyodeoxy | 15018 | 1 |
15018
| 15018
2:2-5 weight feces
6.6
mg
a-muricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
area
521 1246 771 1507 3321 3749 322
response area corrected
% jg bile acid ig bile acid nmol bile acid/
factor for response factor
mg feces mg feces
1.38 718.98
5.2 0.391 59.2
0.145
1
1246
9.0
0.677
102.6
0.261
1
902
6.5
0.490
74.3
0.182
1
1507
10.9
0.819
124.1
0.330
1.08
3586.68
25.9
1.949
295.3
0.723
1
5549
40.1
3.016
456.9
1.118
1
322
2.3
0.175
26.5
0.068
total I 11437 I
I
13831
| 100 | 8 | 1139 |
2.8
nor-hyodeoxy | 18400 | 1 |
18400
| 18400
2:2-6 weight feces
6.4
mg
a-muricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
area
1341 4170 1935 3343 3407 9002 474
response area corrected factor for response factor
% jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
1.38 1850.58
6.4
0.624
97.4
0.238
1
4170
14.3
1.405
219.6
0.559
1
2264
7.8
0.763
119.2
0.292
1
3343
11.5
1.127
176.0
0.467
1.08
3679.56
12.6
1.240
193.7
0.474
1
13323
45.8
4.490
701.5
1.717
1
474
1.6
0.160
25.0
0.064
total I 23672 I
|
29104
| 100 | 10 | 1532 |
3.8
nor-hyodeoxy | 29674 | 1 |
29674
| 29674
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A64 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
3:2-4 weight feces
a -m uricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
6.4
area
469 1577 949 839 2511 4030 280
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
647.22 1577 1110 839
2711.88 5964 280
%
4.9 12.0 8.5 6.4 20.7 45.4 2.1
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.389 0.947
60.7 148.0
0.149 0.377
0.667
104.2
0.255
0.504
78.7
0.209
1.629 3.583 0.168
254.5 559.8 26.3
0.623 1.370 0.067
total
I 10655 I
I
13130
| 100 | 8 | 1232 |
3.0
nor-hyodeoxy | 16647 |
1|
16647
| 16647
3:2-5 weight feces
a-m uricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
6.5
area
438 1197 858 906 3408 2988 364
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
604.44 1197 1004 906
3680.64 4422 364
%
5.0 9.8 8.2 7.4 30.2 36.3 3.0
jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.321 0.635
49.3 97.7
0.121 0.249
0.532 0.481 1.952 2.345 0.193
81.9 73.9 300.3 360.8 29.7
0.200 0.196 0.735 0.883 0.076
total
I 10159 I
I
12178
| 100 | 6 | 994 |
2.5
nor-hyodeoxy I 18855 |
1|
18855
| 18855
3:2-6 weight feces
5.5
mg
a-m uricholate deoxycholate
cholate lithocholate U-muricholate w-muricholate hyodeo/urso
area
871 2878 1791 1999 2933 6248 527
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1201.98 2878 2095 1999
3167.64 9247 527
%
5.7 13.6 9.9 9.5 15.0 43.8 2.5
jg bile acid ig bile acid nmol bile acid/
mg feces mg feces
0.458
83.4
0.204
1.098
199.6
0.508
0.799
145.3
0.356
0.762
138.6
0.368
1.208
219.7
0.538
3.527 0.201
641.2 36.5
1.569 0.093
total
I 17247 I
|
21116
| 100 | 8 | 1464 |
3.6
nor-hyodeoxy | 26219 |
1|
26219
| 26219
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A65 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:3-7___________________________
weight feces
5.8
mg
a-m u rich olate deoxycholate
cholate lithocholate U-muricholate w -m u rich o la te hyodeo/urso
area
463 2160 560 2503 3081 5475 269
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
638.94 2160 655 2503 3327.48 8103 269
%
3.6 12.2 3.7 14.2 18.8 45.9 1.5
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.283 0.956
48.7 164.8
0.119 0.420
0.290
50.0
0.122
1.108
191.0
0.507
1.472 3.586 0.119
253.9 618.2 20.5
0.621 1.513 0.052
total
| 14511 |
I
17657
| 100 | 8 | 1347 |
3~
nor-hyodeoxy | 22598 |
1|
22598
| 22598
1:3-8___________________________
weight feces
5.4
mg
a-m u rich olate deoxycholate
cholate lithocholate U-muricholate w -m u rich o la te hyodeo/urso
area
258 1480 573 1667 1531 3397 212
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
356.04 1480 670 1667
1653.48 5028 212
%
3.2 13.4 6.1 15.1 14.9 45.4 1.9
jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.226 0.939 0.425 1.058 1.049 3.190 0.135
41.8 173.9 78.8 195.9 194.3 590.8 24.9
0.102 0.443 0.193 0.520 0.476 1.446 0.063
total
| 9118 |
I
11066
| 100 | 7 | 1300 |
3~
nor-hyodeoxy I 15759 |
1|
15759
| 15759
1:3-9___________________________
weight feces
6.4
mg
a-m u rich olate deoxycholate
cholate lithocholate U-muricholate w -m u rich o la te hyodeo/urso
area
483 1604 840 2300 2706 3466
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
666.54 1604 983 2300
2922.48 5130 0
%
4.9 11.8 7.2 16.9 21.5 37.7 0.0
jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.414
64.7
0.158
0.997 0.611
155.8 95.5
0.397 0.234
1.430 1.817 3.189 0.000
223.4 283.8 498.2
0.0
0.593 0.695 1.219 0.000
total
| 11399 |
I
13606
| 100 |
8 | 1321 |
3~
nor-hyodeoxy | 16088 |
1|
16088
| 16088
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A66 of A172
Appendix to TNO-report of 3M-02
Final version
2:3-7 weight feces
5
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
213 1811 1070 2080 2392 4005 367
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
293.94 1811 1252 2080
2583.36 5927 367
%
2.1 12.7 8.7 14.5 18.0 41.4 2.6
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.139 0.858
27.9 171.6
0.068 0.437
0.593
118.7
0.290
0.986
197.1
0.523
1.224 2.809 0.174
244.8 561.8 34.8
0.599 1.375 0.089
total I 11938 I
I
14315
| 100 | 7 | 1357 |
~
nor-hyodeoxy | 21102 | 1 |
21102
| 21102
2:3-8______________________
weight feces
7.3
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
222 790 602 1050 1101 2234 135
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
306.36 790 704 1050
1189.08 3306 135
%
4.1 10.6 9.4 14.0 15.9 44.2 1.8
jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.300
41.0
0.100
0.772 0.689
105.8 94.3
0.269 0.231
1.026
140.6
0.373
1.162
159.2
0.390
3.232 0.132
442.8 18.1
1.084 0.046
total I 6134 I
I
7481
| 100 | 7 | 1002 |
~
nor-hyodeoxy | 10229 | 1 |
10229
| 10229
2:3-9______________________
weight feces
5.3
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
887 2496 1947 482 4734 4874
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1224.06 2496 2278 482
5112.72 7214 0
%
6.5 13.3 12.1 2.6 27.2 38.4 0.0
jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.333 0.679 0.620 0.131 1.391 1.963 0.000
62.9 128.2 117.0 24.8 262.5 370.4
0.0
0.154 0.326 0.286 0.066 0.643 0.907 0.000
total I 15420 I
I
18806
| 100 | 5 | 966 |
~
nor-hyodeoxy | 36744 | 1 |
36744
| 36744
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A67 of A172
Appendix to TNO-report of 3M-02
Final version
3:3-7______________________
weight feces
8.2
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
727 3031 1265 1974 4421 5331 401
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1003.26 3031 1480 1974
4774.68 7890 401
%
4.9 14.7 7.2 9.6 23.2 38.4 2.0
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.379 1.146
46.3 139.8
0.113 0.356
0.560 0.747
68.3 91.0
0.167 0.242
1.806 2.984 0.152
220.2 363.9 18.5
0.539 0.891 0.047
total
I 17150 1
I
20554
| 100 | 8 | 948 |
~
nor-hyodeoxy | 26440 | 1 |
26440
| 26440
3:3-8______________________
weight feces
6.4
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
541 1842 998 1904 2601 4796 270
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
746.58 1842 1168 1904 2809.08 7098 270
%
4.7 11.6 7.4 12.0 17.7 44.8 1.7
jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.264
41.3
0.101
0.652 0.413 0.674
101.8 64.5 105.2
0.259 0.158 0.279
0.994
155.3
0.380
2.511 0.096
392.3 14.9
0.960 0.038
total
| 12952 |
|
15837
| 100 | 6 | 875 |
~
nor-hyodeoxy | 28268 | 1 |
28268
| 28268
3:3-9______________________
weight feces
7
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
612 2141 1127 2027 3995 4490 224
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
844.56 2141 1319 2027 4314.6 6645 224
%
4.8 12.2 7.5 11.6 24.6 37.9 1.3
jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.381
54.5
0.133
0.967
138.1
0.352
0.595 0.915
85.0 130.7
0.208 0.347
1.948 3.000 0.101
278.3 428.6 14.4
0.681 1.049 0.037
total
| 14616 1
|
17515
| 100 | 8 | 1130 |
~
nor-hyodeoxy | 22149 | 1 |
22149
| 22149
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A68 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:4-10 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.1
area
103 354 286 394 349 1185 235
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
142.14 354 335 394
376.92 1754 235
%
4.0 9.9 9.3 11.0 10.5 48.8 6.5
|jg bile acid
0.114 0.283 0.268 0.315 0.302 1.404 0.188
ng bile acid/ mg feces
22.3 55.6 52.5 61.8 59.2 275.2 36.9
nmol bile acid/ mg feces
0.055 0.142 0.129 0.164 0.145 0.674 0.094
total
I 2906 I
I 3590 | 100 | 3 | 563 | ~ T
nor-hyodeoxy | 12494 | 1 |
12494
| 12494
1:4-11 weight feces
4.8
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
276 396 184 214 555 896
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
380.88 396 215 214 599.4 1326 0
%
12.2 12.6 6.9 6.8 19.1 42.3 0.0
|jg bile acid
0.454 0.472 0.257 0.255 0.715 1.581 0.000
ng bile acid/ mg feces
94.6 98.3 53.5 53.1 148.9 329.3 0.0
nmol bile acid/ mg feces
0.231 0.250 0.131 0.141 0.364 0.806 0.000
total
I 2521 I
I 3132 | 100 | 4 | 778 | T
nor-hyodeoxy
8389
1:4-12 weight feces
6
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
total
I
area
320 852 475 954 954 2059
5614
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
441.6 852 556 954 1030.32 3047
0
| I 6881 |
%
6.4 12.4 8.1 13.9 15.0 44.3 0.0
100
|jg bile acid ng bile acid/ nmol bile acid/
mg feces
mg feces
0.390 0.753 0.491 0.843 0.911 2.694 0.000
65.1 125.5 81.9 140.6 151.8 449.0
0.0
0.159 0.320 0.200 0.373 0.372 1.099 0.000
1 .5| 6 | 1014 |
nor-hyodeoxy | 11311 | 1 |
11311
| 11311
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A69 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:4-10 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.2
area
361 1051 1247 1420 979 2197
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
498.18 1051 1459 1420
1057.32 3252 0
%
5.7 12.0 16.7 16.3 12.1 37.2 0.0
|jg bile acid
0.258 0.544 0.755 0.735 0.547 1.682 0.000
ng bile acid/ mg feces
41.6 87.7 121.7 118.5 88.2 271.3 0.0
nmol bile acid/ mg feces
0.102 0.223 0.298 0.315 0.216 0.664 0.000
total
I 7255 I
| 8737 | 100 | 5 | 729 | ~ T ^
nor-hyodeoxy | 19330 | 1 |
19330
| 19330
2:4-11 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.6
area
842 1126 866 1217 2527 3746 481
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1161.96 1126 1013 1217
2729.16 5544 481
%
8.8 8.5 7.6 9.2 20.6 41.8 3.6
|jg bile acid
0.510 0.494 0.444 0.534 1.197 2.432 0.211
ng bile acid/ mg feces
77.2 74.8 67.3 80.9 181.4 368.4 32.0
nmol bile acid/ mg feces
0.189 0.191 0.165 0.215 0.444 0.902 0.081
total
I 10805 |
| 13272 | 100 | 6 | 882 | T ?
nor-hyodeoxy | 22800 | 1 |
22800
| 22800
2:4-12 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
7.6
area
543 902 1180 1019 1622 2624 206
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
749.34 902 1381 1019
1751.76 3884 206
%
7.6 9.1 14.0 10.3 17.7 39.3 2.1
|jg bile acid
0.555 0.668 1.023 0.755 1.297 2.876 0.153
ng bile acid/ mg feces
73.0 87.9 134.5 99.3 170.7 378.5 20.1
nmol bile acid/ mg feces
0.179 0.224 0.329 0.264 0.418 0.926 0.051
total
I 8096 |
| 9892 | 100 | 7 | 964 | H *
nor-hyodeoxy | 13502 | 1 |
13502
| 13502
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A70 of A172
Appendix to TNO-report of 3M-02
Final version
3:4-10 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
8.1
area
374 928 737 749 1189 2355 244
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
516.12 928 862 749
1284.12 3485 244
%
6.4 11.5 10.7 9.3 15.9 43.2 3.0
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.307
38.0
0.093
0.553
68.2
0.174
0.514
63.4
0.155
0.446
55.1
0.146
0.765 2.076 0.145
94.4 256.3 17.9
0.231 0.627 0.046
total
I 6576 I
|
8069
| 100 | 5 | 593 |
1~
nor-hyodeoxy | 16790 | 1 |
16790
| 16790
3:4-11 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
8.3
area
474 1184 335 668 2030 2413 257
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
654.12 1184 392 668
2192.4 3571 257
%
7.3 13.3 4.4 7.5 24.6 40.0 2.9
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.475 0.860 0.285 0.485 1.592 2.593 0.187
57.2 103.6 34.3 58.4 191.8 312.5 22.5
0.140 0.264 0.084 0.155 0.469 0.765 0.057
total
I 7361 |
I
8919
| 100 | 6 | 780 |
1~
nor-hyodeoxy | 13770 | 1 |
13770
| 13770
3:4-12 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.6
area
436 831 1182 693 1774 2161 172
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
601.68 831 1383 693
1915.92 3198 172
%
6.8 9.4 15.7 7.9 21.8 36.4 2.0
|jg bile acid ig bile acid nmol bile acid/ mg feces mg feces
0.394 0.544
59.7 82.4
0.146 0.210
0.905 0.454 1.254
137.1 68.7 190.0
0.336 0.182 0.465
2.093 0.113
317.1 17.1
0.776 0.043
total
I 7249 I
|
8795
| 100 | 6 | 872 |
2~
nor-hyodeoxy | 15281 | 1 |
15281
| 15281
04-12-2006
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A71 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
| 1:5-13 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.1
area
279 1398 233 1493 1698 1962 138
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
385.02 1398 273 1493
1833.84 2904 138
%
4.6 16.6 3.2 17.7 21.8 34.5 1.6
|jg bile acid
0.194 0.706 0.138 0.754 0.926 1.466 0.070
ng bile acid/ mg feces
38.1 138.4 27.0 147.8 181.5 287.4 13.7
nmol bile acid/ mg feces
0.093 0.352 0.066 0.392 0.444 0.703 0.035
total
| 7201 |
| 8424 | 100 | 4 | 834 | ~ 2 T
nor-hyodeoxy | 19809 | 1 |
19809
| 19809
| 1:5-14 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.9
area
273 1027 1007 1318 1369 1924 193
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
376.74 1027 1178 1318
1478.52 2848 193
%
4.5 12.2 14.0 15.7 17.6 33.8 2.3
|jg bile acid
0.202 0.550 0.631 0.706 0.792 1.526 0.103
ng bile acid/ mg feces
34.2 93.3 107.0 119.7 134.3 258.6 17.5
nmol bile acid/ mg feces
0.084 0.238 0.262 0.318 0.329 0.633 0.045
total
| 7111 |
| 8419 | 100 | 5 | 764 | " T ?
nor-hyodeoxy | 18666 | 1 |
18666
| 18666
| 1:5-15 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.6
area
201 976 457 1197 374 2027
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
277.38 976 535 1197
403.92 3000
0
%
4.3 15.3 8.4 18.7 6.3 47.0 0.0
|jg bile acid
0.239 0.842 0.461 1.033 0.349 2.589 0.000
ng bile acid/ mg feces
36.3 127.6 69.9 156.5 52.8 392.3
0.0
nmol bile acid/ mg feces
0.089 0.325 0.171 0.416 0.129 0.960 0.000
total
| 5232 |
| 6389 | 100 | 6 | 836 | ~ 2 T
nor-hyodeoxy | 11586 | 1 |
11586
| 11586
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A72 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
| 2:5-13 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.9
area
170 421 188 1014 816 778 196
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
234.6 421 220 1014 881.28 1151 196
%
5.7 10.2 5.3 24.6 21.4 28.0 4.8
|jg bile acid
0.289 0.519 0.271 1.249 1.086 1.418 0.241
ng bile acid/ mg feces
41.9 75.2 39.3 181.0 157.3 205.6 35.0
nmol bile acid/ mg feces
0.103 0.191 0.096 0.481 0.385 0.503 0.089
total
| 3583 |
| 4118 | 100 | 5 | 735 |
nor-hyodeoxy | 8118 | 1 |
8118
| 8118
| 2:5-14 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
7.1
area
488 1599 1339 1652 2345 2835 357
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
673.44 1599 1567 1652
2532.6 4196 357
%
5.4 12.7 12.5 13.1 20.1 33.4 2.8
|jg bile acid
0.342 0.812 0.795 0.838 1.285 2.130 0.181
ng bile acid/ mg feces
48.1 114.3 112.0 118.1 181.0 299.9 25.5
nmol bile acid/ mg feces
0.118 0.291 0.274 0.314 0.443 0.734 0.065
total
| 10615 |
|
12576
| 100 | 6 | 899 | ~ 2 T
nor-hyodeoxy | 19702 | 1 |
19702
| 19702
| 2:5-15 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.4
area
1147 3831 1371 3141 2207 7603 531
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1582.86 3831 1604 3141
2383.56 11252 531
%
6.5 15.7 6.6 12.9 9.8 46.3 2.2
|jg bile acid
0.461 1.115 0.467 0.914 0.694 3.275 0.155
ng bile acid/ mg feces
85.3 206.5 86.5 169.3 128.5 606.5 28.6
nmol bile acid/ mg feces
0.209 0.526 0.212 0.450 0.314 1.484 0.073
total
| 19831 |
|
24326
| 100 |
7 | 1311 | ~ 3 ^
nor-hyodeoxy | 34357 | 1 |
34357
| 34357
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A73 of A172
Appendix to TNO-report of 3M-02
Final version
3:5-13_________________________
weight feces
7.9
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
608 1723 925 1896 2481 3000 400
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
839.04 1723 1082 1896
2679.48 4440 400
%
6.4 13.2 8.3 14.5 20.5 34.0 3.1
|jg bile acid
0.282 0.580 0.364 0.638 0.901 1.493 0.135
ng bile acid/ mg feces
35.7 73.4 46.1 80.7 114.1 189.0 17.0
nmol bile acid/ mg feces
0.087 0.187 0.113 0.214 0.279 0.463 0.043
total I 11033 ]
I
13060
| 100 | 4 | 556 |
~
nor-hyodeoxy | 29729 | 1 |
29729
| 29729
3:5-14_________________________
weight feces
8.1
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
391 1007 1069 799 1999 2126 548
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
539.58 1007 1251 799
2158.92 3146 548
%
5.7 10.7 13.2 8.5 22.8 33.3 5.8
jg bile acid
0.264 0.493 0.613 0.391 1.058 1.542 0.268
ng bile acid/ mg feces
32.6 60.9 75.7 48.3 130.6 190.3 33.1
nmol bile acid/ mg feces
0.080 0.155 0.185 0.128 0.320 0.466 0.084
total I 7939 1
|
9450
| 100 | 5 | 572 |
~
nor-hyodeoxy | 20411 | 1 |
20411
| 20411
3:5-15 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
7.3
area
908 3513 1190 2079 2885 6815 481
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1253.04 3513 1392 2079 3115.8 10086 481
%
5.7 16.0 6.4 9.5 14.2 46.0 2.2
jg bile acid
0.363 1.019 0.404 0.603 0.903 2.924 0.139
ng bile acid/ mg feces
49.8 139.5 55.3 82.6 123.7 400.6 19.1
nmol bile acid/ mg feces
0.122 0.355 0.135 0.219 0.303 0.980 0.049
total | 17871 1
|
21920
| 100 |
6 | 871 |
2T
nor-hyodeoxy | 34491 | 1 |
34491
| 34491
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A74 of A172
Appendix to TNO-report of 3M-02
Final version
1:6-16 weight feces
5.8
mg
a-muricholate deoxycholate
cholate lithocholate
B-muricholate w-muricholate hyodeo/urso
area
222 654 724 565 752 1281 123
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
306.36 654 847 565
812.16 1896 123
%
5.9 12.6 16.3 10.9 15.6 36.4 2.4
|jg bile acid
0.188 0.401 0.519 0.346 0.498 1.162 0.075
ng bile acid/ mg feces
32.4 69.1 89.5 59.7
85.8 200.4 13.0
nmol bile acid/ mg feces
0.079 0.176 0.219 0.159
0.210 0.490 0.033
total I 4321 I
I
5203
| 100 |
3 | 550 | T ?
nor-hyodeoxy | 16312 | 1 |
16312
| 16312
1:6-17 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
6.2
area
199 543 411 518 383 1077 111
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
274.62 543 481 518
413.64 1594 111
%
7.0 13.8 12.2 13.2 10.5 40.5 2.8
jg bile acid
0.250 0.494 0.438 0.472 0.377 1.451 0.101
ng bile acid/ mg feces
40.3 79.7 70.6 76.1 60.7 234.1 16.3
nmol bile acid/ mg feces
0.099 0.203 0.173 0.202 0.149 0.573 0.042
total I 3242 I
|
3935
| 100 |
4 | 578 | T ?
nor-hyodeoxy I 10983 | 1 |
10983
| 10983
1:6-18 weight feces
6.3
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
117 650 962 598 677 1575 174
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
161.46 650 1126 598
731.16 2331 174
%
2.8 11.3 19.5 10.4 12.7 40.4 3.0
jg bile acid
0.120 0.483 0.837 0.444 0.543 1.732 0.129
ng bile acid/ mg feces
19.0 76.7 132.8 70.5 86.3 275.0 20.5
nmol bile acid/ mg feces
0.047 0.195 0.325 0.187 0.211 0.673 0.052
total I 4753 I
| 5771 | 100 | 4 | 681 | T 7
nor-hyodeoxy I 13455 | 1 |
13455
| 13455
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A75 of A172
Appendix to TNO-report of 3M-02
Final version
2:6-16 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.6
area
215 821 747 598 840 1244 226
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
296.7 821 874 598 907.2 1841 226
%
5.3 14.8 15.7 10.7 16.3 33.1 4.1
|jg bile acid
0.169 0.469 0.499 0.341 0.518 1.051 0.129
ng bile acid/ mg feces
30.2 83.7 89.1 60.9 92.5 187.6 23.0
nmol bile acid/ mg feces
0.074 0.213 0.218 0.162 0.226 0.459 0.059
total
I 4691 I
|
5564
| 100 |
3 | 567 |
~
nor-hyodeoxy | 17522 | 1 |
17522
| 17522
2:6-17 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.5
area
196 966 220 486 177 733
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
270.48 966 257 486
191.16 1085
0
%
8.3 29.7 7.9 14.9 5.9 33.3 0.0
|jg bile acid
0.241 0.862 0.230 0.434 0.171 0.968 0.000
ng bile acid/ mg feces
43.9 156.7 41.8 78.8 31.0 176.0
0.0
nmol bile acid/ mg feces
0.107 0.399 0.102 0.209 0.076 0.431 0.000
total
I 2778 I
|
3256
| 100 |
3 | 528 |
~
nor-hyodeoxy | 11207 | 1 |
11207
| 11207
2:6-18 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
7.4
area
553 1729 1319 1154 1500 3330 318
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
763.14 1729 1543 1154 1620 4928 318
%
6.3 14.3 12.8 9.6 13.4 40.9 2.6
|jg bile acid
0.365 0.827 0.738 0.552 0.775 2.358 0.152
ng bile acid/ mg feces
49.3 111.8 99.8 74.6 104.7 318.6 20.6
nmol bile acid/ mg feces
0.121 0.285 0.244 0.198 0.256 0.780 0.052
total
I 9903 I
I
12056
| 100 |
6 | 779 |
~
nor-hyodeoxy | 20902 | 1 |
20902
| 20902
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A76 of A172
Appendix to TNO-report of 3M-02
Final version
3:6-16 weight feces
7.4
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
621 1219 1628 685 1439 1866 253
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
856.98 1219 1905 685
1554.12 2762 253
%
9.3 13.2 20.6 7.4 16.8 29.9 2.7
|jg bile acid
0.369 0.525 0.820 0.295 0.669 1.189 0.109
ng bile acid/ mg feces
49.9 70.9 110.8 39.9 90.4 160.7 14.7
nmol bile acid/ mg feces
0.122 0.181 0.271 0.106 0.221 0.393 0.038
total I 7711 I
I 9235 | 100 | 4 | 537 | " T ?
nor-hyodeoxy | 23222 | 1 |
23222
| 23222
3:6-17 weight feces
8.1
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
829 1831 1171 653 1120 4227 295
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1144.02 1831 1370 653 1209.6 6256 295
%
9.0 14.4 10.7 5.1 9.5 49.0 2.3
jg bile acid
0.427 0.683 0.511 0.243 0.451 2.332 0.110
ng bile acid/ mg feces
52.7 84.3 63.1 30.1 55.7 288.0 13.6
nmol bile acid/ mg feces
0.129 0.215 0.154 0.080 0.136 0.705 0.035
total I 10126 I
I 12759 | 100 | 5 | 587 |
nor-hyodeoxy I 26821 | 1 |
26821
| 26821
3:6-18 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
7.7
area
441 1194 995 999 934 1799
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
608.58 1194 1164 999
1008.72 2663 0
%
8.0 15.6 15.2 13.1 13.2 34.9 0.0
jg bile acid
0.253 0.497 0.484 0.416 0.420 1.108 0.000
ng bile acid/ mg feces
32.9 64.5 62.9 54.0 54.5 143.9 0.0
nmol bile acid/ mg feces
0.080 0.164 0.154 0.143 0.133 0.352 0.000
total I 6362 I
| 7637 | 100 | 3 | 413 | ~ T
nor-hyodeoxy I 24030 | 1 |
24030
| 24030
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A77 of A172
Appendix to TNO-report of 3M-02
Final version
1:7-19_________________________
weight feces
5.2
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
493 1997 1424 376 1518 1175
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
680.34 1997 1666 376
1639.44 1739 0
%
8.4 24.7 20.6 4.6 20.2 21.5 0.0
|jg bile acid
0.556 1.631 1.361 0.307 1.339 1.421 0.000
ng bile acid/ mg feces
106.9 313.7 261.7 59.1 257.6 273.2
0.0
nmol bile acid/ mg feces
0.262 0.799 0.641 0.157 0.630 0.669 0.000
total
| 6983 |
|
8098
| 100 |
7 | 1272 |
~
nor-hyodeoxy | 12241 | 1 |
12241
| 12241
1:7-20_________________________
weight feces
5.8
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
602 895 817 302 659 977 267
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
830.76 895 956 302
711.72 1446 267
%
15.4 16.5 17.7 5.6 13.2 26.7 4.9
|jg bile acid
0.725 0.781 0.834 0.264 0.621 1.262 0.233
ng bile acid/ mg feces
125.0 134.7 143.8 45.4 107.1 217.6 40.2
nmol bile acid/ mg feces
0.306 0.343 0.352 0.121 0.262 0.533 0.102
total
| 4519 |
| 5408 | 100 | 5 | 814 | ~
nor-hyodeoxy | 11457 | 1 |
11457
| 11457
1:7-21 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.4
area
512 1419 1545 649 893 912 204
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
706.56 1419 1808 649 964.44 1350 204
%
10.0 20.0 25.5 9.1 13.6 19.0 2.9
|jg bile acid
0.525 1.055 1.344 0.482 0.717 1.003 0.152
ng bile acid/ mg feces
97.3 195.3 248.8 89.3 132.8 185.8 28.1
nmol bile acid/ mg feces
0.238 0.498 0.609 0.237 0.325 0.455 0.072
total
| 6134 |
nor-hyodeoxy | 13452 |
| 1|
7100 13452
| 100 | 5 | 977 | ~ | 13452
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A78 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:7-19__________________________
weight feces
5.5
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
216 631 176 110 543 386
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
298.08 631 206 110
586.44 571 0
%
12.4 26.3 8.6 4.6 24.4 23.8 0.0
|jg bile acid
0.542 1.148 0.375 0.200 1.067 1.039 0.000
ng bile acid/ mg feces
98.6 208.7 68.1 36.4 194.0 189.0
0.0
nmol bile acid/ mg feces
0.241 0.532 0.167 0.097 0.475 0.463 0.000
total
| 2062 |
| 2403 | 100 | 4 | 795 | T
nor-hyodeoxy | 5496 | 1 |
5496
| 5496
2:7-20__________________________
weight feces
5.9
mg
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
area
872 3015 1451 382 2004 3004 653
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1203.36 3015 1698 382
2164.32 4446 653
%
8.9 22.2 12.5 2.8 16.0 32.8 4.8
|jg bile acid
0.443 1.110 0.625 0.141 0.797 1.637 0.240
ng bile acid/ mg feces
75.1 188.2 106.0 23.8 135.1 277.5 40.8
nmol bile acid/ mg feces
0.184 0.479 0.259 0.063 0.331 0.679 0.104
total
| 11381 |
| 13561 | 100 | 5 | 846 | ~~2T
nor-hyodeoxy | 27158 | 1 |
27158
| 27158
2:7-21 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
5.3
area
665 3474 1201 397 1600 2024 155
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
917.7 3474 1405 397 1728 2996 155
%
8.3 31.4 12.7 3.6 15.6 27.1 1.4
|jg bile acid
0.585 2.215 0.896 0.253 1.102 1.910 0.099
ng bile acid/ mg feces
110.4 418.0 169.1 47.8 207.9 360.4 18.6
nmol bile acid/ mg feces
0.270 1.065 0.414 0.127 0.509 0.882 0.047
total
| 9516 |
| 11072 | 100 | 7 | 1332 | ~ 3 ^
nor-hyodeoxy | 15683 | 1 |
15683
| 15683
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A79 of A172
Appendix to TNO-report of 3M-02
Final version
3:7-19_________________________
weight feces
6.7
mg
a-muricholate deoxycholate
cholate lithocholate
B-muricholate w-muricholate
hyodeo/urso
area
535 850 877 325 1150 921 605
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
738.3 850 1026 325
1242 1363 605
%
12.0 13.8 16.7 5.3 20.2 22.2 9.8
|jg bile acid
0.631 0.726 0.877 0.278 1.061 1.164 0.517
ng bile acid/ mg feces
94.1 108.4 130.8 41.4
158.4 173.8 77.1
nmol bile acid/ mg feces
0.230 0.276 0.320 0.110
0.388 0.425 0.196
total
I 5263 !
nor-hyodeoxy | 11706 |
| 1|
6149 11706
| 100 | 5 | 784 | | 11706
1.9
3:7-20_________________________
weight feces
6.5
mg
oppervlakte
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
290 571 462 258 300 422
response factor
1.38 1 1 1
1.08 1 1
oppervlakte
400.2 571 541 258 324 625
0
%
14.7 21.0 19.9 9.5 11.9 23.0 0.0
jg bile acid
0.950 1.355 1.283 0.612 0.769 1.482 0.000
ng bile acid/ mg faeces
146.1 208.5 197.3 94.2 118.3 228.0
0.0
nmol bile acid/ mg faeces
0.358 0.531 0.483 0.250 0.289 0.558 0.000
total
| 2303 1
|
nor-hyodeoxy | 4214 | 1 |
2718 4214
| 100 | 6 | 992 | | 4214
2.5
3:7-21 weight feces
a-muricholate deoxycholate
cholate lithocholate B-muricholate w-muricholate hyodeo/urso
7.2
area
1184 3208 2501 1182 3049 3105 1201
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1633.92 3208 2926 1182
3292.92 4595 1201
%
9.1 17.8 16.2 6.6 18.3 25.5 6.7
jg bile acid
0.503 0.987 0.900 0.364 1.013 1.414 0.370
ng bile acid/ mg feces
69.8 137.1 125.1 50.5 140.7 196.4 51.3
nmol bile acid/ mg feces
0.171 0.349 0.306 0.134 0.344 0.481 0.131
total
| 15430 1
|
18039
| 100 | 6 | 771 |
1.9
nor-hyodeoxy | 32495 | 1 |
32495
| 32495
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A80 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A81 of A172
group
1
AVERAGE SD 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3
AVERAGE SD 4
4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 AVERAGE SD
cage#
1,2 3 ,4
5 1,2 3 ,4
5 1,2 3 ,4
5
6 7 ,8 9,1 0
6 7 ,8 9,1 0
6 7 ,8 9,1 0
1 1 ,1 2 ,1 3 15
1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7
18 19 20 ,2 1 18 19 20 ,2 1 18 19 20 ,2 1
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
2 8 ,2 9 31
3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
GC sa m p le #
1:1-1 1 :1 -2 1 :1 -3 2:1-1 2:1 -2 2:1 -3 3:1-1 3:1 -2 3:1 -3
a -m u ric h o la te 0.18 9 0.11 3 0.12 0 0.31 9 0.16 0 0.21 7 0.11 2 0.12 0 0.17 2
d e o x y c h o la te 0.53 2 0.27 4 0 .3 1 1 0.52 8 0.30 5 0.36 6 0.76 4 0.29 8 0.33 0
1 :2 -4 1 :2 -5 1 :2 -6 2:2 -4 2:2 -5 2:2 -6 3:2 -4 3:2 -5 3:2 -6
0.08 2 0.08 0 0.15 0 0.16 5 0.14 5 0.23 8 0.14 9 0 .1 2 1 0.20 4
0.34 5 0.27 8 0.49 6 0.39 6 0 .2 6 1 0.55 9 0.37 7 0.24 9 0.50 8
1 :3 -7 1 :3 -8 1 :3 -9 2:3 -7 2:3 -8 2:3 -9 3:3 -7 3:3 -8 3:3 -9
0.11 9 0.10 2 0.15 8 0.06 8 0.10 0 0.15 4 0.11 3 0 .1 0 1 0.13 3
0.42 0 0.44 3 0.39 7 0.43 7 0.26 9 0.32 6 0.35 6 0.25 9 0.35 2
1 :4-10 1 :4 -1 1 1 :4-12 2 :4 -1 0 2 :4 -1 1 2 :4 -1 2 3 :4 -1 0 3 :4 -1 1 3 :4 -1 2
0.05 5 0 .2 3 1 0.15 9 0.10 2 0.18 9 0.17 9 0.09 3 0.14 0 0.14 6
0.14 2 0.25 0 0.32 0 0.22 3 0 .1 9 1 0.22 4 0.17 4 0.26 4 0.21 0
1 :5-13 1 :5-14 1 :5-15 2 :5 -1 3 2 :5 -1 4 2 :5 -1 5 3 :5 -1 3 3 :5 -1 4 3 :5 -1 5
0.09 3 0.08 4 0.08 9 0.10 3 0.11 8 0.20 9 0.08 7 0.08 0 0.12 2
0.35 2 0.23 8 0.32 5 0 .1 9 1 0 .2 9 1 0.52 6 0.18 7 0.15 5 0.35 5
1 :6-16 1 :6-17 1 :6-18 2 :6 -1 6 2 :6 -1 7 2 :6 -1 8 3 :6 -1 6 3 :6 -1 7 3 :6 -1 8
0.07 9 0.09 9 0.04 7 0.07 4 0.10 7 0 .1 2 1 0.12 2 0.12 9 0.08 0
0.17 6 0.20 3 0.19 5 0.21 3 0.39 9 0.28 5 0 .1 8 1 0.21 5 0.16 4
1 :7-19 1 :7-20 1 :7 -2 1 2 :7 -1 9 2 :7 -2 0 2 :7 -2 1 3 :7 -1 9 3 :7 -2 0 3 :7 -2 1
0.26 2 0.30 6 0.23 8 0 .2 4 1 0.18 4 0.27 0 0.23 0 0.35 8 0 .1 7 1
0.79 9 0.34 3 0.49 8 0.53 2 0.47 9 1.06 5 0.27 6 0 .5 3 1 0.34 9
bile acid s (um o l/g feces) c h o la te lith o c h o l a te b -m u ric h o la te w -m u ric h o la te h y o d e o /u rs o
0.17 4
0.27 2
0.69 7
1 .0 1 2
0.07 6
0.22 7
0.40 5
0.40 7
1 .4 3 5
0.04 6
0.17 9
0.41 3
0.40 6
1 .2 8 0
0.05 7
0.18 4
0.26 8
0.63 9
0.55 3
0.00 0
0.31 7
0.33 9
0.55 8
1 .5 5 7
0.02 5
0 .4 1 1
0.09 6
0.64 5
1 .2 2 9
0.02 9
0.12 9
0 .1 4 1
0.79 7
0.97 6
0.03 9
0 .1 6 1 0.22 7
0.29 7 0.17 6
0.37 6 0.62 8
1 .0 5 9 1 .0 3 8
0.03 8 0.06 9
bile a cid s to ta l (um ol/g feces)
2.9 5 2 .9 1 2.7 7 2.4 9 3.2 6 2.9 9 2.9 6 2.3 5 2.6 4
to ta l feces
(g) 3.07 5 2 .48 97 2 .29 46 4.69 2 3 .77 89 3 .41 06 3 .57 54 2 .8 7 8 1 2 .75 89
a ve rag e w eig ht days
(g) 12 3.6 8 7 .2 5 8 8 .5 5 1 2 2.45 8 6 .5 5 8 8 .2 1 2 1.65 8 6 .5 5 8 8 .0 5
2 2 2 3 3 3 2 2 2
0.25 5 0.25 2 0.27 5 0.22 9 0.18 2 0.29 2 0.25 5 0.20 0 0.35 6
0.24 7 0.32 2 0.56 6 0.29 8 0.33 0 0.46 7 0.20 9 0.19 6 0.36 8
0.36 6 0 .8 2 1 0.46 7 0.44 0 0.72 3 0.47 4 0.62 3 0.73 5 0.53 8
1 .1 6 2 0.94 4 1 .7 3 4 1 .2 1 9 1 .1 1 8 1 .7 1 7 1 .3 7 0 0.88 3 1 .5 6 9
0.06 2 0.02 4 0.05 9 0.00 0 0.06 8 0.06 4 0.06 7 0.07 6 0.09 3
2.5 2 2.7 2 3.7 5 2.7 5 2.8 3 3 .8 1 3.0 5 2.4 6 3.6 4
2 .76 19 4 .0 5 8 1 2 .09 35 4.07 5 6 .3 7 1 3 .40 74 2 .92 79 4 .39 85 2 .45 75
9 4 .5 5 12 6.6 8 2 .2 9 4 .2 5 1 2 6.45 82 .1
94 12 6.6 8 1 .8 5
2 2 2 3 3 3 2 2 2
0.12 2 0.19 3 0.23 4 0.29 0 0 .2 3 1 0.28 6 0.16 7 0.15 8 0.20 8
0.50 7 0.52 0 0.59 3 0.52 3 0.37 3 0.06 6 0.24 2 0.27 9 0.34 7
0 .6 2 1 0.47 6 0.69 5 0.59 9 0.39 0 0.64 3 0.53 9 0.38 0 0 .6 8 1
1 .5 1 3 1 .4 4 6 1 .2 1 9 1 .3 7 5 1 .0 8 4 0.90 7 0 .8 9 1 0.96 0 1 .0 4 9
0.05 2 0.06 3 0.00 0 0.08 9 0.04 6 0.00 0 0.04 7 0.03 8 0.03 7
3.3 6 3.2 4 3.3 0 3.3 8 2.4 9 2.3 8 2.3 5 2.1 8 2 .8 1
3 .45 97 2 .29 94 2 .34 54 5 .81 47 3 .77 18 3 .77 27 4.02 5 2 .52 65 2 .69 05
118 8 3 .5 5 8 2 .8 1 1 8.75 8 3 .5 8 3 .0 5 11 9.6 8 4 .2 8 3 .4 5
2 2 2 3 3 3 2 2 2
0.12 9 0 .1 3 1 0.20 0 0.29 8 0.16 5 0.32 9 0.15 5 0.08 4 0.33 6
0.16 4 0 .1 4 1 0.37 3 0.31 5 0.21 5 0.26 4 0.14 6 0.15 5 0.18 2
0.14 5 0.36 4 0.37 2 0.21 6 0.44 4 0.41 8 0 .2 3 1 0.46 9 0.46 5
0.67 4 0.80 6 1 .0 9 9 0.66 4 0.90 2 0.92 6 0.62 7 0.76 5 0.77 6
0.09 4 0.00 0 0.00 0 0.00 0 0 .0 8 1 0 .0 5 1 0.04 6 0.05 7 0.04 3
1 .4 0 1 .9 2 2.5 2 1 .8 2 2.1 9 2.3 9 1 .4 7 1 .9 3 2.1 6
1.68 9 3 .14 32 3 .25 45 2 .71 33 4 .64 07 5 .20 75 2 .0 4 1 3 .44 64 3 .95 49
5 9 .5 11 6.4 11 2.2 5 8 .8 5 1 1 6.55 1 1 1.85 5 8 .7 1 1 7.45 11 2.9
2 2 2 3 3 3 2 2 2
0.06 6 0.26 2 0 .1 7 1 0.09 6 0.27 4 0.21 2 0.11 3 0.18 5 0.13 5
0.39 2 0.31 8 0.41 6 0 .4 8 1 0.31 4 0.45 0 0.21 4 0.12 8 0.21 9
0.44 4 0.32 9 0.12 9 0.38 5 0.44 3 0.31 4 0.27 9 0.32 0 0.30 3
0.70 3 0.63 3 0.96 0 0.50 3 0.73 4 1 .4 8 4 0.46 3 0.46 6 0.98 0
0.03 5 0.04 5 0.00 0 0.08 9 0.06 5 0.07 3 0.04 3 0.08 4 0.04 9
2.0 9 1.91 2.0 9 1 .8 5 2.2 4 3.2 7 1 .3 9 1 .4 2 2.1 6
3.19 5 2 .94 08 2 .63 99 4 .97 12 4 .48 33 3.76 7 3 .61 13 3 .35 22 2 .70 34
11 0.7 8 8 .3 5 8 4 .6 11 1.6 8 8 .5 8 5 .5 5 1 1 2.85 8 8 .9 8 5 .5
2 2 2 3 3 3 2 2 2
0.21 9 0.17 3 0.32 5 0.21 8 0.10 2 0.24 4 0 .2 7 1 0.15 4 0.15 4
0.15 9 0.20 2 0.18 7 0.16 2 0.20 9 0.19 8 0.10 6 0.08 0 0.14 3
0.21 0 0.14 9 0 .2 1 1 0.22 6 0.07 6 0.25 6 0 .2 2 1 0.13 6 0.13 3
0.49 0 0.57 3 0.67 3 0.45 9 0 .4 3 1 0.78 0 0.39 3 0.70 5 0.35 2
0.03 3 0.04 2 0.05 2 0.05 9 0.00 0 0.05 2 0.03 8 0.03 5 0.00 0
1 .3 7 1 .4 4 1 .6 9 1.41 1 .3 2 1 .9 4 1 .3 3 1 .4 5 1 .0 3
2 .50 36 2 .70 83 2 .35 54 3 .96 84 3 .96 78 3 .56 66 2 .90 76 3 .24 49 2 .76 43
87 .1 11 2.8 8 5 .4 8 7 .1 5 1 1 2.45 8 4 .5 5 8 7 .6 5 1 1 1.95 8 4 .6 5
2 2 2 3 3 3 2 2 2
0 .6 4 1 0.35 2 0.60 9 0.16 7 0.25 9 0.41 4 0.32 0 0.48 3 0.30 6
0.15 7 0 .1 2 1 0.23 7 0.09 7 0.06 3 0.12 7 0.11 0 0.25 0 0.13 4
0.63 0 0.26 2 0.32 5 0.47 5 0 .3 3 1 0.50 9 0.38 8 0.28 9 0.34 4
0.66 9 0.53 3 0.45 5 0.46 3 0.67 9 0.88 2 0.42 5 0.55 8 0 .4 8 1
0.00 0 0.10 2 0.07 2 0.00 0 0.10 4 0.04 7 0.19 6 0.00 0 0 .1 3 1
3.1 6 2.0 2 2.4 3 1 .9 7 2.1 0 3 .3 1 1 .9 5 2.4 7 1 .9 2
1.96 97 2 .07 98 2 .67 05 3 .44 77 3 .26 46 4 .30 59 2 .51 17 2 .43 69 3 .41 07
8 1 .3 5 8 2 .2 5 1 0 1.05 8 2 .3 5 8 2 .7 5 1 0 1.95 8 3 .5 8 3 .3 5 10 3.2
2 2 2 3 3 3 2 2 2
to tal b ile acid excre tion (um ol/1 00 gr m ou se/d ay)
3 .6 7 4 .1 5 3 .5 8 3 .1 8 4 .7 5 3 .8 6 4 .3 5 3 .9 0 4 .1 4 3 .9 5 0 .4 6 3 .6 8 4 .3 6 4 .7 7 3 .9 6 4 .7 5 5 .2 7 4 .7 5 4 .2 7 5 .4 6 4 .5 9 0 .5 8 4 .9 2 4 .4 6 4 .6 7 5 .5 2 3 .7 5 3.61 3 .9 6 3 .2 6 4 .5 3 4 .3 0 0.71 1 .9 9 2 .6 0 3 .6 6 2 .7 9 2 .9 0 3.71 2 .5 6 2 .8 4 3 .7 8 2 .9 8 0.61 3.01 3 .1 7 3 .2 6 2 .7 4 3 .7 8 4 .8 0 2 .2 2 2 .6 7 3 .4 2 3 .2 3 0 .7 4 1 .9 6 1 .7 3 2 .3 3 2 .1 4 1 .5 6 2 .7 2 2.21 2.11 1 .6 8 2 .0 5 0 .3 6 3 .8 2 2 .5 5 3 .2 2 2 .7 5 2 .7 6 4 .6 7 2 .9 3 3.61 3 .1 7 3 .2 7 0 .6 7
% o f b ile acid s
1
a -m u ric h o la te d e o x y c h o la te c h o la te lith o c h o la te b -m u ric h o la te w -m u ric h o la te h y o d e o /u rs o
6.3 9
1 8 .0 3
5.8 9
9 .2 1
23 .6 1
3 4 .3 0
2 .5 7
3.8 7 4.3 3
9.4 2 1 1 .2 4
7.8 0 6.4 6
1 3 .9 5 1 4 .9 4
1 4 .0 0 1 4 .6 8
4 9 .3 7 4 6 .2 9
1 .5 8 2 .0 6
12 .8 2
2 1 .1 9
7.3 7
1 0 .7 5
2 5 .6 6
2 2 .2 0
0 .0 0
4.9 2
9.3 4
9 .7 1
10 .4 1
1 7 .1 0
4 7 .7 5
0 .7 8
7.2 4 3.7 8
1 2 .2 3 2 5 .8 5
1 3 .7 5 4.3 7
3.2 2 4.7 6
2 1 .5 4 2 6 .9 4
4 1 .0 6 3 2 .9 9
0 .9 7 1 .3 2
5.1 3 6.5 3
1 2 .6 8 12 .5 1
6.8 4 8.6 0
1 2 .6 3 6.6 6
1 6 .0 0 2 3 .7 8
45 .1 1 3 9 .3 2
1.61 2 .6 0
6 .1 1 2.8 0
1 4 .7 2 5 .7 1
7.8 7 2.6 9
9 .6 1 4.0 5
2 0 .3 7 4.9 7
3 9 .8 2 8.7 5
1 .5 0 0 .8 5
3.2 6
1 3 .6 9
1 0 .1 3
9.8 0
1 4 .5 3
4 6 .1 3
2 .4 6
2.9 3
1 0 .2 3
9.2 6
1 1 .8 4
3 0 .1 7
3 4 .7 0
0 .8 7
4.0 0 6.0 0
1 3 .2 4 1 4 .4 3
7.3 5 8.3 3
15 .1 1 1 0 .8 5
1 2 .4 6 1 6 .0 2
4 6 .2 8 4 4 .3 8
1 .5 7 0 .0 0
5.1 3
9.2 5
6.4 3
1 1 .6 6
2 5 .5 8
3 9 .5 7
2 .3 9
6.2 6
1 4 .6 7
7.6 5
1 2 .2 6
1 2 .4 4
4 5 .0 4
1 .6 7
4.8 7
1 2 .3 6
8.3 6
6.8 6
2 0 .4 3
4 4 .9 2
2 .1 9
4 .9 1 5 .6 1
10 .1 1 1 3 .9 8
8.1 5 9.7 8
7.9 8 1 0 .1 2
2 9 .8 8 1 4 .7 8
3 5 .9 0 4 3 .1 6
3 .0 8 2 .5 6
4.7 7 1 .1 6
1 2 .4 4 2.0 6
8.3 8 1 .1 9
1 0 .7 2 2.4 3
1 9 .5 9 7.2 3
4 2 .2 3 4.4 2
1 .8 6 0 .9 5
3.5 6
12 .5 1
3.6 5
15 .1 1
1 8 .5 2
4 5 .1 0
1 .5 6
3.1 6 4 .8 1
1 3 .6 6 1 2 .0 4
5.9 4 7.0 9
1 6 .0 4 1 8 .0 0
1 4 .6 6 2 1 .0 8
4 4 .5 8 3 6 .9 9
1 .9 6 0 .0 0
2.0 2
1 2 .9 3
8.5 9
1 5 .4 8
1 7 .7 2
4 0 .6 5
2 .6 2
4.0 3
10 .8 1
9.2 6
1 4 .9 7
1 5 .6 3
4 3 .4 6
1 .8 5
6.4 6 4 .8 1
13 .7 1 1 5 .1 2
1 2 .0 2 7.0 9
2.7 6 1 0 .2 7
2 6 .9 8 2 2 .8 9
3 8 .0 7 3 7 .8 2
0 .0 0 2 .0 0
4.6 4 4.7 5
1 1 .9 2 1 2 .5 3
7.2 6 7 .4 1
1 2 .8 4 1 2 .3 7
1 7 .4 6 2 4 .2 6
4 4 .1 3 3 7 .3 7
1 .7 5 1.31
4.2 5 1 .2 6
1 2 .8 0 1 .2 5
7.5 9 2.3 0
1 3 .0 9 4.4 9
19 .9 1 4.1 5
40 .9 1 3.4 2
1 .4 5 0 .9 0
3.9 0
1 0 .1 0
9.1 7
1 1 .7 2
1 0 .3 3
4 8 .0 8
6 .7 0
12 .0 3
1 3 .0 2
6.8 0
7.3 3
1 8 .9 3
4 1 .8 9
0 .0 0
6 .3 1
1 2 .6 7
7.9 4
1 4 .7 9
1 4 .7 3
4 3 .5 5
0 .0 0
5.6 0 8.6 4
1 2 .2 9 8.7 2
1 6 .3 9 7.5 4
17 .3 1 9.8 2
1 1 .8 8 2 0 .3 0
3 6 .5 3 4 1 .2 5
0 .0 0 3 .7 2
7.4 8
9.3 7
1 3 .7 7
1 1 .0 3
1 7 .4 8
3 8 .7 4
2 .1 4
6 .3 1
11 .8 1
1 0 .5 4
9.9 3
1 5 .7 0
42 .6 1
3 .1 0
7.2 4 6.7 6
1 3 .6 4 9.7 2
4.3 4 1 5 .5 5
8.0 2 8.4 5
2 4 .2 7 2 1 .5 4
3 9 .5 3 3 5 .9 6
2 .9 6 2.01
7.1 4 2.2 6
1 1 .2 6 1 .8 0
1 0 .2 3 4.1 7
1 0 .9 4 3.2 8
1 7 .2 4 4.5 5
4 0 .9 0 3.7 5
2 .2 9 2 .1 9
4.4 7 4.3 9
1 6 .8 9 1 2 .4 6
3.1 6 1 3 .7 3
18 .8 1 1 6 .6 7
2 1 .2 9 1 7 .2 3
33 .7 1 3 3 .1 9
1 .6 7 2 .3 4
4.2 5
1 5 .5 5
8.1 9
1 9 .8 9
6.1 9
4 5 .9 4
0 .0 0
5.5 5
1 0 .3 6
5.2 0
26 .0 1
2 0 .8 4
2 7 .2 2
4 .8 2
5.2 6 6.3 9
1 3 .0 0 1 6 .1 0
1 2 .2 4 6.4 8
14 .0 1 1 3 .7 6
1 9 .7 9 9.6 2
3 2 .7 9 4 5 .4 3
2 .9 0 2 .2 3
6 .3 1
1 3 .4 8
8.1 3
1 5 .4 6
2 0 .1 4
3 3 .3 6
3 .1 3
5.6 3 5.6 3
1 0 .9 4 1 6 .4 3
1 3 .0 5 6.2 5
9.0 5 1 0 .1 3
2 2 .5 3 1 4 .0 0
3 2 .8 4 45 .3 1
5 .9 5 2 .2 5
5.3 2 0.8 0
13 .9 1 2.4 3
8.4 9 3.7 2
1 5 .9 7 5.1 9
1 6 .8 5 5.7 2
3 6 .6 4 6.9 6
2.81 1 .7 4
5.8 0
1 2 .8 8
1 6 .0 3
1 1 .6 0
1 5 .3 7
3 5 .8 9
2 .4 2
6.8 6
14 .1 1
1 2 .0 0
1 4 .0 3
1 0 .3 3
3 9 .7 9
2 .8 8
2.7 6 5.2 4
1 1 .5 5 1 5 .1 0
1 9 .2 2 1 5 .4 5
1 1 .0 8 1 1 .4 7
1 2 .4 9 1 6 .0 4
39 .8 1 3 2 .5 4
3 .0 9 4 .1 6
8 .1 1
3 0 .1 3
7 .7 1
1 5 .8 0
5.7 3
32 .5 1
0 .0 0
6.2 4
1 4 .7 0
12 .6 1
1 0 .2 3
1 3 .2 4
4 0 .2 7
2 .7 0
9.1 6 8.8 7
1 3 .5 6 1 4 .7 7
2 0 .3 7 1 0 .6 2
7.9 5 5.4 9
1 6 .6 2 9.3 8
2 9 .5 3 4 8 .4 9
2 .8 2 2 .3 8
7.8 3
1 5 .9 9
1 4 .9 8
1 3 .9 5
1 2 .9 8
3 4 .2 6
0 .0 0
6.7 6 2.0 2
1 5 .8 7 5 .5 1
1 4 .3 3 4.0 4
1 1 .2 9 3.1 8
1 2 .4 6 3 .5 1
37 .0 1 5.7 3
2 .2 7 1 .3 9
8.2 9 15 .1 6
25 .3 1 1 6 .9 9
2 0 .2 9 1 7 .4 4
4.9 7 5.9 8
1 9 .9 7 1 2 .9 8
2 1 .1 8 2 6 .3 8
0 .0 0 5 .0 7
9.7 8
2 0 .4 5
2 5 .0 3
9.7 5
1 3 .3 5
1 8 .6 9
2 .9 4
12 .2 3
2 6 .9 4
8.4 5
4.9 0
2 4 .0 6
2 3 .4 3
0 .0 0
8.7 6
2 2 .8 3
1 2 .3 5
3.0 2
1 5 .7 5
3 2 .3 5
4 .9 5
8.1 5 11 .8 4
3 2 .1 3 1 4 .1 9
1 2 .4 9 1 6 .4 5
3.8 3 5.6 5
1 5 .3 5 1 9 .9 2
2 6 .6 2 2 1 .8 6
1 .4 3 1 0 .1 0
14 .4 8 8.9 2
2 1 .5 0 1 8 .2 3
1 9 .5 6 1 5 .9 7
1 0 .1 3 7.0 0
1 1 .7 2 1 7 .9 7
2 2 .6 0 2 5 .0 8
0 .0 0 6 .8 2
10 .8 4 2.6 9
2 2 .0 6 5.5 0
1 6 .4 5 4.9 5
6.1 4 2.4 5
1 6 .7 9 4.0 2
2 4 .2 4 3.9 7
3 .4 8 3 .5 4
Appendix to TNO-report of 3M-02
Final version
04-12-2006
bile acid
total bile acids a-muricholate deoxycholate
cholate lithocholate b-muricholate w-muricholate hyodeo/urso
bile acid
total bile acids a-muricholate deoxycholate
cholate lithocholate b-muricholate w-muricholate hyodeo/urso
bile acid
total bile acids a-muricholate deoxycholate
cholate lithocholate b-muricholate w-muricholate hyodeo/urso
group 1 vs 2 nn p 9 9 0.019 9 9 0.258 9 9 0.863 9 9 0.340 9 9 0.605 9 9 0.666 9 9 0.931 9 9 0.436 group 2 vs 4
nn p 9 9 <0.001 9 9 0.006 9 9 0.113 9 9 0.546 9 9 0.931 9 9 0.605 9 9 0.387 9 9 0.730 group 3 vs 7 nn p 9 9 0.008 9 9 <0.001 9 9 <0.001 9 9 <0.001 9 9 0.004 9 9 0.161 9 9 <0.001 9 9 0.387
group 1 vs 3 nn p 9 9 0.297 9 9 0.077 9 9 0.931 9 9 1.000 9 9 0.063 9 9 1.000 9 9 0.730 9 9 1.000 group 2 vs 5 nn p 9 9 0.003 9 9 0.297 9 9 0.222 9 9 0.666 9 9 0.019 9 9 0.666 9 9 0.094 9 9 0.258 group 4 vs 5 nn p 9 9 0.489 9 9 0.031 9 9 0.031 9 9 0.340 9 9 0.031 9 9 1.000 9 9 0.161 9 9 0.546
Bile acid excretion, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5 group 1 vs 6
nn E nn p nn
p
9 9 0.003 9 9 0.019 9 9 <0.001
9 9 0.190 9 9 0.863 9 9 0.258
9 9 0.340 9 9 0.796 9 9 0.258
9 9 0.190 9 9 0.931 9 9 0.002
9 9 0.666 9 9 0.019 9 9 0.340
9 9 0.258 9 9 0.190 9 9 0.003
9 9 1.000 9 9 0.258 9 9 0.297
9 9 0.387 9 9 0.040 9 9 0.094
group 2 vs 6 group 2 vs 7 group 3 vs 4
nn p nn p nn
p
9 9 <0.001 9 9 0.001 9 9 0.002
9 9 0.024 9 9 <0.001 9 9 0.004
9 9 0.040 9 9 <0.001 9 9 0.094
9 9 0.001 9 9 <0.001 9 9 0.161
9 9 0.730 9 9 0.002 9 9 0.094
9 9 0.077 9 9 0.546 9 9 0.387
9 9 0.063 9 9 <0.001 9 9 0.863
9 9 0.258 9 9 0.546 9 9 0.222
group 4 vs 6 group 4 vs 7 group 5 vs 6
nn p nn p nn
p
9 9 0.002 9 9 0.489 9 9 <0.001
9 9 1.000 9 9 0.003 9 9 0.063
9 9 0.003 9 9 <0.001 9 9 0.730
9 9 0.077 9 9 0.011 9 9 0.014
9 9 0.666 9 9 0.003 9 9 0.077
9 9 0.040 9 9 0.796 9 9 0.050
9 9 0.094 9 9 <0.001 9 9 0.931
9 9 0.931 9 9 0.605 9 9 0.931
group 1 vs 7 nn p 9 9 0.019 9 9 0.002 9 9 0.014 9 9 0.001 9 9 0.077 9 9 0.136 9 9 0.001 9 9 0.387 group 3 vs 5 nn p 9 9 0.008 9 9 0.094 9 9 0.340 9 9 0.796 9 9 0.387 9 9 0.340 9 9 0.258 9 9 0.031 group 5 vs 7 nn p 9 9 0.863 9 9 <0.001 9 9 <0.001 9 9 0.002 9 9 <0.001 9 9 0.666 9 9 <0.001 9 9 0.863
group 2 vs 3 nn p 9 9 0.387 9 9 0.258 9 9 1.000 9 9 0.222 9 9 0.040 9 9 0.605 9 9 0.436 9 9 0.340 group 3 vs 6 nn p 9 9 <0.001 9 9 0.008 9 9 0.063 9 9 0.001 9 9 0.161 9 9 0.001 9 9 0.094 9 9 0.050 group 6 vs 7 nn p 9 9 <0.001 9 9 0.002 9 9 0.011 9 9 0.340 9 9 0.002 9 9 0.050 9 9 <0.001 9 9 0.546
p<0.05
Page A82 of A172
Appendix to TNO-report of 3M-02
Final version
E3. Fecal sterol measurements
1:1-1 weight feces
6.2
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon
1 0 0.0 0.0 0 0.00 1 0 0.0 0.0 0 0.00
cholesterol 284344
0.98
278657
85.2
60.3
9718
25.13
cholestanol lathosterol
9225 10102
0.91 1
8395 10102
2.6 1.8 293 0.75 3.1 2.2 352 0.91
campesterol 4671
1.56
7287
2.2 1.6 254 0.63
stigmasterol
1682
1.49
2506
0.8 0.5 87 0.21
b-sitosterol b-sitostanol
13713
1.47 1
20158 0
6.2 4.4 703
1.70
0.0 0.0 0 0.00
total I 323737 I
| 327105 | 100 | 71 | 11407 | 29.3 |
cholestane | 11117^ | "
| 115625
|
______________________________________115625_____________________________________________
| 1:1-2
~
weight feces
6.5
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon cholesterol 313994
1 0.98
0 307714
0.0 0.0 0 0.00 86.6 76.7 11803 30.52
cholestanol
9355
0.91
8513
2.4 2.1 327 0.84
lathosterol campesterol
9015 4796
1 1.56
9015 7482
2.5 2.2 346 0.89 2.1 1.9 287 0.72
stigmasterol
1808
1.49
2694
0.8 0.7 103 0.25
b-sitosterol b-sitostanol
13562
1.47 1
19936 0
5.6 5.0 765
1.84
0.0 0.0 0 0.00
total I 352530 |
| 355354 | 100 | 89 | 13631 | 35.1 |
cholestane | 96413 | 4 |
100270
|
100270_____________________________________________
1 1 3 ________________________________________________________________________
weight feces
7.1
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon cholesterol cholestanol
298829 8558
1 0.98 0.91
0 292852
7788
0.0 0.0 0 0.00 86.7 73.5 10347 26.76 2.3 2.0 275 0.71
lathosterol campesterol stigmasterol
10265 4356 1589
1 1.56 1.49
10265 6795 2368
3.0 2.6 363 0.94 2.0 1.7 240 0.60 0.7 0.6 84 0.20
b-sitosterol b-sitostanol
12044
1.47 1
17705 0
5.2 4.4 626
1.51
0.0 0.0 0 0.00
total I 335641 |
| 337773 | 100 | 85 | 1193^ | 30.7 |
cholestane 95828
1.04
99661 99661
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A83 of A172
Appendix to TNO-report of 3M-02
Final version
| 2:1-1 weight feces
7
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol
stigmasterol
b-sitosterol b-sitostanol
area
319680 11436 10467 5023 1268 13626
response area corrected factor for response factor
10 10 0.98 313286 0.91 10407 1 10467 1.56 7836 1.49 1889 1.47 20030 10
%
0.0 0.0 86.1 2.9 2.9 2.2 0.5 5.5 0.0
pg sterol
0.0 0.0 87.7 2.9 2.9 2.2 0.5 5.6 0.0
ng sterol/ mg feces
0 0 12532 416 419 313 76 801 0
nmol sterol/ mg feces
0.00 0.00 32.41 1.07 1.08 0.78 0.18 1.93 0.00
total | 361500 !
| 363916 | 100 | 102 | 14557 | 37.5 |
cholestane | 85850 | 1.04 |
| 2:1-2 weight feces
10.4
mg
89284 89284
~
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon cholesterol 508256
1 0.98
0 498091
0.0 0.0
0
87.4
131.0
12591
0.00 32.56
cholestanol 15011
0.91
13660
2.4 3.6 345
0.89
lathosterol campesterol
13004 7404
1 1.56
13004 11550
2.3 3.4 329 2.0 3.0 292
0.85 0.73
stigmasterol b-sitosterol b-sitostanol
2781 19988
1.49 1.47
1
4144 29382
0
0.7 1.1 105 0.25 5.2 7.7 743 1.79 0.0 0.0 0 0.00
total | 566444 |
| 569831 | 100 | 150 | 14405 | 37.1 |
cholestane | 91434 | 1.04 |
| 2:1-3 weight feces
6.8
mg
9509~ 95091
'
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 292666
0.98
286813
86.8
89.9
13215
34.17
cholestanol lathosterol
8651 9600
0.91 1
7872 9600
2.4 2.5 363 2.9 3.0 442
0.93 1.14
campesterol 4193
1.56
6541
2.0 2.0 301
0.75
stigmasterol 1436
1.49
2140
0.6 0.7
99
0.24
b-sitosterol b-sitostanol
11876
1.47 1
17458 0
5.3 5.5 804
0.0 0.0
0
1.94 0.00
total | 328422 |
| 330424 | 100 | 104 | 15225 | 39.2 |
cholestane 76721 | 1.04
79790 79790
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A84 of A172
Appendix to TNO-report of 3M-02
Final version
3:1-1____________________
weight feces
6.1
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol cholestanol lathosterol
440429 13442 11803
1 1 0.98 0.91 1
0 0 431620 12232 11803
0.0 0.0 0 0.00
0.0 0.0 0 0.00
87.6 73.9 12121 31.34
2.5 2.1 344 0.88
2.4 2.0 331
0.86
campesterol 6241
1.56
9736
2.0 1.7 273 0.68
stigmasterol 2114
1.49
3150
0.6 0.5 88 0.21
b-sitosterol b-sitostanol
16460
1.47 1
24196 0
4.9 4.1 679
0.0 0.0
0
1.64 0.00
total I 490489 I
| 492738 | 100 | 84 | 13837 | 35.6 |
cholestane | 140330 | 4 |
145943 145943
|
3 H 2 ________________________________________________________________________
weight feces
5.3
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon
1 0 0.0 0.0 0 0.00 1 0 0.0 0.0 0 0.00
cholesterol cholestanol lathosterol campesterol
237491 7441 6444 3777
0.98 0.91
1 1.56
232741 6771 6444 5892
86.7 55.6 10481 27.10 2.5 1.6 305 0.78 2.4 1.5 290 0.75 2.2 1.4 265 0.66
stigmasterol b-sitosterol b-sitostanol
982 10254
1.49 1.47
1
1463 15073
0
0.5 0.3
66
5.6 3.6 679
0.0 0.0
0
0.16 1.64 0.00
total | 266389 |
| 268385 | 100 | 64 | 12086 | 31.1 |
cholestane | 100714 | 4 |
104743 104743
|
3 3 ________________________________________________________________________
weight feces
6.7
mg
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
404372 11102 11373 5436 2003 14146
response area corrected factor for response factor
10 10 0.98 396285 0.91 10103 1 11373 1.56 8480 1.49 2984 1.47 20795 10
%
0.0 0.0 88.1 2.2 2.5 1.9 0.7 4.6 0.0
pg sterol
0.0 0.0 72.6 1.9 2.1 1.6 0.5 3.8 0.0
ng sterol/ mg feces
0 0 10838 276 311 232 82 569 0
nmol sterol/ mg feces
0.00 0.00 28.03 0.71 0.80 0.58 0.20 1.37 0.00
total | 448432 |
| 450020 | 100 | 82 | 12307 | 31.7 |
cholestane 131190
1.04
136438 136438
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A85 of A172
Appendix to TNO-report of 3M-02
Final version
1:2-4 weight feces
9.1
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
473290 9709 12754 6031 2406 17298
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 463824 8835 12754 9408 3585 25428 0
%
0.0 0.0 88.5 1.7 2.4 1.8 0.7 4.9 0.0
^ig sterol
0.0 0.0 123.6 2.4 3.4 2.5 1.0 6.8 0.0
ng sterol/ mg feces
0 0 13579 259 373 275 105 744 0
nmol sterol/ mg feces
0.00 0.00 35.12 0.67 0.97 0.69 0.25 1.80 0.00
total I 521488 I
| 523835 | 100 | 140 | 15336 | 39.5 |
cholestane | 90227 | 1.04 |
1:2-5 weight feces
7.2
mg
93836 93836
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
446804 6124 14209 4560 1811 12767
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 437868 5573 14209 7114 2698 18767 0
%
0.0 0.0 90.1 1.1 2.9 1.5 0.6 3.9 0.0
^g sterol
0.0 0.0 114.1 1.5 3.7 1.9 0.7 4.9 0.0
ng sterol/ mg feces
0 0 15846 202 514 257 98 679 0
nmol sterol/ mg feces
0.00 0.00 40.98 0.52 1.33 0.64 0.24 1.64 0.00
total I 486275 I
| 486229 | 100 | 127 | 17596 | 45.3 |
cholestane | 92255 | 1.04 |
1:2-6 weight feces
6.2
mg
95945 95945
|
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
298782 11596 8473 5240 1956 15108
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 292806 10552 8473 8174 2914 22209 0
%
0.0 0.0 84.8 3.1 2.5 2.4 0.8 6.4 0.0
^g sterol
0.0 0.0 65.1 2.3 1.9 1.8 0.6 4.9 0.0
ng sterol/ mg feces
0 0 10502 378 304 293 105 797 0
nmol sterol/ mg feces
0.00 0.00 27.16 0.97 0.79 0.73 0.25 1.92 0.00
total I 341155 I
| 345129 | 100 | 77 | 12379 | 31.8 |
cholestane 108095
1.04
112419 112419
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A86 of A172
Appendix to TNO-report of 3M-02
Final version
2:2-4 w eight feces
8 .6
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol c am pestero l stigm asterol b-sitosterol b-sitostanol
area
481673 11693 12978 6203 1175 17089
response factor
1 1 0 .9 8 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 472040 10641 12978 9677 1751 25121 0
%
0 .0 0 .0 88.7 2 .0 2 .4 1.8 0 .3 4 .7 0 .0
pg sterol
0 .0 0 .0 118.2 2 .7 3 .3 2 .4 0 .4 6 .3 0 .0
ng sterol/ mg feces
0 0 13750 310 378 282 51 732 0
nmol sterol/ mg feces
0 .0 0 0 .0 0 35.56 0 .8 0 0 .9 8 0 .7 0 0 .1 2 1.76 0 .0 0
total
I 530811 I
I 532206
| 100
| 133 | 15502 |
39.9 ~ |
cholestane I 95962 | "
|
2:2-5 w eight feces
9 .9
mg
998 99800
|
coprostanol coprostanon cholesterol cholestanol
lathosterol c am pestero l stigm asterol b-sitosterol b-sitostanol
area
434117 8812 13243 5232 2068 14440
response area corrected factor for response factor
1 1 0 .9 8 0.91 1 1.56 1.49 1.47 1
0 0 425435 8019 13243 8162 3081 21227 0
%
0 .0 0 .0 88.8 1.7 2 .8 1.7 0 .6 4 .4 0 .0
pg sterol
0 .0 0 .0 152.2 2 .9 4 .7 2 .9 1.1 7.6 0 .0
ng sterol/ mg feces
0 0 15373 290 479 295 111 767 0
nmol sterol/ mg feces
0 .0 0 0 .0 0 39.75 0 .7 5 1.24 0 .7 4 0 .2 7 1.85 0 .0 0
total
I 477912 I
| 479167
| 100 | 171 | 1731 ^ | 4 4.6 ~ |
cholestane | 6 71 9 6 | "
|
2:2-6 w eight feces
6
mg
69884 69884
|
coprostanol coprostanon cholesterol cholestanol
lathosterol c am pestero l stigm asterol b-sitosterol b-sitostanol
area
268071 10152 6784 4476 1045 12736
response area corrected factor for response factor
1 1 0 .9 8 0.91 1 1.56 1.49 1.47 1
0 0 262710 9238 6784 6983 1557 18722 0
%
0 .0 0 .0 85.9 3 .0 2.2 2 .3 0 .5 6.1 0 .0
pg sterol
0 .0 0 .0 72.3 2 .5 1.9 1.9 0 .4 5.1 0 .0
ng sterol/ mg feces
0 0 12043 423 311 320 71 858 0
nmol sterol/ mg feces
0 .0 0 0 .0 0 31.14 1.09 0 .8 0 0 .8 0 0 .1 7 2 .0 7 0 .0 0
total
I 303264 I
| 3 05 9 9 3 | 100 | 84 | 14027 | 36.1 ~ |
cholestane [ 8 74 0 0 | 1.04 |
90896 90896
|
coprostanol coprostanon cholesterol cholestanol
lathosterol c am pestero l stigm asterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A87 of A172
Appendix to TNO-report of 3M-02
Final version
3:2-4 weight feces
5.6
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol
b-sitosterol b-sitostanol
area
269271 7132 7058 3976 1401 10601
response factor
1 1 0.98 0.91 1 1.56 1.49
1.47 1
area corrected for response factor
0 0 263886 6490 7058 6203 2087
15583 0
%
0.0 0.0 87.6 2.2 2.3 2.1 0.7 5.2 0.0
pg sterol
0.0 0.0 66.6 1.6 1.8 1.6 0.5 3.9 0.0
ng sterol/ mg feces
0 0 11896 293 318 280 94
702 0
nmol sterol/ mg feces
0.00 0.00 30.76 0.75 0.82 0.70 0.23
1.69 0.00
total
I 299439 I
| 301307 | 100 | 76 | 13582 | 35.0 |
cholestane | 95225 | "
|
:2-5 weight feces
6.9
mg
99034 99034
|
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
398275 7905 11474 4821 1507 11865
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 390310 7194 11474 7521 2245 17442 0
%
0.0 0.0 89.5 1.6 2.6 1.7 0.5 4.0 0.0
pg sterol
0.0 0.0 79.9 1.5 2.3 1.5 0.5 3.6 0.0
ng sterol/ mg feces
0 0 11581 213 340 223 67 518 0
nmol sterol/ mg feces
0.00 0.00 29.95 0.55 0.88 0.56 0.16 1.25 0.00
total
I 435847 I
| 436185
| 100 | 89 | 12942 | 33.3 |
cholestane | 11741^ | "
|
:2-6 weight feces
7.2
mg
122111 122111
|
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
336997 12422 7963 5484 2009 15075
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 330257 11304 7963 8555 2993 22160 0
%
0.0 0.0 86.2 2.9 2.1 2.2 0.8 5.8 0.0
pg sterol
0.0 0.0 80.7 2.8 1.9 2.1 0.7 5.4 0.0
ng sterol/ mg feces
0 0 11206 384 270 290 102 752 0
nmol sterol/ mg feces
0.00 0.00 28.98 0.99 0.70 0.72 0.25 1.81 0.00
total I 379950 |
| 383233 | 100 | 94 | 13003 | 33.4 |
cholestane 98396
1.04
102332 102332
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A88 of A172
Appendix to TNO-report of 3M-02
Final version
| 1:3-7 weight feces
6
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol
240788
1 1 0.98
0 0 235972
0.0 0.0 0 0.00 0.0 0.0 0 0.00 85.5 67.4 11229 29.04
cholestanol lathosterol
7267 9283
0.91 1
6613 9283
2.4 1.9 315 0.81
3.4 2.7 442
1.14
campesterol 3808
1.56
5940
2.2 1.7 283 0.71
stigmasterol 1488
1.49
2217
0.8 0.6 106 0.26
b-sitosterol b-sitostanol
10778
1.47 1
15844 0
5.7 4.5 754
1.82
0.0 0.0 0 0.00
total | 273412 |
| 275869 | 100 | 79 | 13128 | 33.8 |
cholestane | 84191 | 1.04 |
87559 87559
|
13-8 weight feces
_______________________________________________________________________________ 7 mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 283543
0.98
277872
85.5 71.7 10245 26.49
cholestanol 10969
0.91
9982
3.1 2.6 368 0.95
lathosterol
8285
1
8285
2.5 2.1 305 0.79
campesterol 4663
1.56
7274
2.2 1.9 268 0.67
stigmasterol 1774
1.49
2643
0.8 0.7
97
0.24
b-sitosterol 12953
1.47
19041
5.9 4.9 702
1.69
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 322187 I
| 325097
| 100 | 84 | 1198^ | 30.8 |
cholestane | 93139 | 4 |
96865
____________________________________96865
1:3-9
weight feces 10.8
mg
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol cholestanol
529012 14038
1 1 0.98 0.91
0 0 518432 12775
0.0 0.0 0 0.00
0.0 0.0 0 0.00
89.0
153.4
14205
36.73
2.2 3.8 350 0.90
CM CO
lathosterol 11702
1
11702
2.0 3.5
0.83
campesterol stigmasterol b-sitosterol b-sitostanol
6647 1317 18688
1.56 1.49 1.47
1
10369 1962 27471
0
1.8 3.1 284 0.71
0.3 0.6
54
0.13
4.7 8.1 753 1.82
0.0 0.0 0 0.00
total I 581404 I
| 582711 | 100 | 172 | 15966 | 41.1 |
cholestane 81233
1.04
84482 84482
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A89 of A172
Appendix to TNO-report of 3M-02
Final version
| 2:3-7 weight feces
8
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 330311
0.98
323705
86.2 95.0 11875 30.71
cholestanol 9369
0.91
8526
2.3 2.5 313
0.80
lathosterol
11675
1
11675
3.1 3.4 428
1.11
campesterol 5157
1.56
8045
2.1 2.4 295 0.74
stigmasterol 1936
1.49
2885
0.8 0.8 106 0.26
b-sitosterol 14114
1.47
20748
5.5 6.1 761
1.84
b-sitostanol
1 0 0.0 0.0 0 0.00
total | 372562 1
| 375583 | 100 | 110 | 13778 | 35.4 |
cholestane | 81910 | 1.04 |
| 2:3-8 weight feces
7.3
mg
85186 85186
|
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon
1 0 0.0 0.0 0 0.00 1 0 0.0 0.0 0 0.00
cholesterol cholestanol
361988 10955
0.98 0.91
354748 9969
87.7 89.9 12322 31.86
2.5 2.5 346
0.89
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
10492 4873 1595 13274
1 1.56 1.49 1.47
1
10492 7602 2377 19513
0
2.6 2.7 364
1.9 1.9 264
0.6 0.6
83
4.8 4.9 678
0.0 0.0
0
0.94 0.66 0.20 1.63 0.00
total | 403177 1
| 404701 | 100 | 103 | 14057 | 36.2 |
cholestane | 94804 | 1.04 |
| 2:3-9 weight feces
7.7
mg
98596 98596
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol
409014
1 1 0.98
0 0 400834
0.0 0.0
0
0.0 0.0
0
88.8
109.4
14211
0.00 0.00 36.75
cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
11289 9106 5174 1042 14716
0.91 1
1.56 1.49 1.47
1
10273 9106 8071 1553 21633
0
2.3 2.8 364
2.0 2.5 323
1.8 2.2 286
0.3 0.4
55
4.8 5.9 767
0.0 0.0
0
0.94 0.83 0.71 0.13 1.85 0.00
total | 450341 1
| 451469 | 100 | 123 | 16006 | 41.2 |
cholestane 88054
1.04
91576 91576
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A90 of A172
Appendix to TNO-report of 3M-02
Final version
| 3:3-7 weight feces
8.2
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol
stigmasterol
b-sitosterol b-sitostanol
area
428635 12166 13406 6496 2441 16860
response area corrected factor for response factor
10 10 0.98 420062 0.91 11071 1 13406 1.56 10134 1.49 3637 1.47 24784 10
%
0.0 0.0 87.0 2.3 2.8 2.1 0.8 5.1 0.0
pg sterol
0.0 0.0 95.1 2.5 3.0 2.3 0.8 5.6 0.0
ng sterol/ mg feces
0 0 11598 306 370 280 100 684 0
nmol sterol/ mg feces
0.00 0.00 29.99 0.79 0.96 0.70 0.24 1.65 0.00
total | 480004 1
| 483094
| 100 | 109 | 13338 | 34.3 |
cholestane | 10617^ | 1.04 |
| 3:3-8 weight feces
7.6
mg
110421 110421
|
|
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
370433 13556 9317 6050 2043 15825
response area corrected factor for response factor
10 10 0.98 363024 0.91 12336 1 9317 1.56 9438 1.49 3044 1.47 23263 10
%
0.0 0.0 86.3 2.9 2.2 2.2 0.7 5.5 0.0
pg sterol
0.0 0.0 74.7 2.5 1.9 1.9 0.6 4.8 0.0
ng sterol/ mg feces
0 0 9828 334 252 256 82 630 0
nmol sterol/ mg feces
0.00 0.00 25.41 0.86 0.65 0.64 0.20 1.52 0.00
total | 417224 1
| 420422 | 100 | 87 | 1138^ | 29.3 |
cholestane | 116833 | "
|
| 3:3-9 weight feces
6
mg
121506 121506
|
|
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
327522 9565 7411 4458 1015 11869
response area corrected factor for response factor
10 10 0.98 320972 0.91 8704 1 7411 1.56 6954 1.49 1512 1.47 17447 10
%
0.0 0.0 88.4 2.4 2.0 1.9 0.4 4.8 0.0
pg sterol
0.0 0.0 71.3 1.9 1.6 1.5 0.3 3.9 0.0
ng sterol/ mg feces
0 0 11877 322 274 257 56 646 0
nmol sterol/ mg feces
0.00 0.00 30.71 0.83 0.71 0.64 0.14 1.56 0.00
total | 361840 1
| 363001 | 100 | 81 | 13432 | 34.6 |
cholestane | 108272 | 1.04 |
112603 112603
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A91 of A172
Appendix to TNO-report of 3M-02
Final version
1:4-10 weightfeces 5.9
mg
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 362101 0.98
354859
88.7 81.6 13829 35.76
cholestanol 9381 0.91
8537
2.1 2.0 333 0.86
lathosterol 7899 1
7899 2.0 1.8 308 0.80
campesterol 4622 1.56
7210
1.8 1.7 281 0.70
stigmasterol 1882 1.49
2804
0.7 0.6 109 0.26
b-sitosterol 12850 1.47
18890
4.7 4.3 736 1.78
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 398735 I
| 400199 | 100 | 92 | 15595 | 40.2 |
cholestane | 104552 | 4 |
1:4-11 weightfeces 8
mg
108734 | 108734
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 489702 0.98
479908
90.0 107.0 13380 34.60
cholestanol 15062 0.91
13706
2.6 3.1 382 0.98
lathosterol 10888 1 10888 2.0 2.4 304 0.78
campesterol 4869 1.56
7596
1.4 1.7 212 0.53
stigmasterol 748 1.49
1115
0.2 0.2 31 0.08
b-sitosterol 13552 1.47
19921
3.7 4.4 555 1.34
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 534821 I
| 533134 | 100 | 119 | 14864 | 38.3 |
cholestane j 107776 | 1.04 |
1:4-12 weightfeces 6.1
mg
112087 | 112087
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 459088 0.98
449906
89.5 96.7 15857 41.01
cholestanol 15039 0.91
13685
2.7 2.9 482 1.24
lathosterol 11182 1 11182 2.2 2.4 394 1.02
campesterol 4797 1.56
7483
1.5 1.6 264 0.66
stigmasterol 686 1.49
1022
0.2 0.2 36 0.09
b-sitosterol 13085 1.47
19235
3.8 4.1 678 1.63
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 503877 I
| 502514 | 100 | 108 | 1771^ | 45.6 |
cholestane 111810 1.04
116282 116282
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A92 of A172
Appendix to TNO-report of 3M-02
Final version
2:4-10 weight feces
7.7
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 521217
0.98
510793
90.7
142.2
18473
47.77
cholestanol lathosterol
14915 11055
0.91 1
13573 11055
2.4 3.8 491 2.0 3.1 400
1.26 1.03
campesterol 4779
1.56
7455
1.3 2.1 270 0.67
stigmasterol
743
1.49
1107
0.2 0.3
40
0.10
b-sitosterol 13109
1.47
19270
3.4 5.4 697
1.68
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 565818 1
| 563253
| 100 | 157 | 20370 | 52.5 |
cholestane j 86322 | 1.04 |
2:4-11 weight feces
6.4
mg
89775 89775
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 497491
0.98
487541
90.8
118.8
18567
48.01
cholestanol 13351
0.91
12149
2.3 3.0 463
1.19
lathosterol
10137
1
10137
1.9 2.5 386
1.00
campesterol 4786
1.56
7466
1.4 1.8 284 0.71
stigmasterol b-sitosterol
727 12456
1.49 1.47
1083 18310
0.2 0.3
41
3.4 4.5 697
0.10 1.68
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 538948 1
| 536687 | 100 | 131 | 20439 | 52.7 |
cholestane j 98627 | 1.04 |
2:4-12 weight feces
8.9
mg
102572 102572
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
572090 15045 12013 5734 676 15286
1 1 0.98 0.91 1 1.56 1.49 1.47 1
0 0 560648 13691 12013 8945 1007 22470 0
0.0 0.0 0 0.00
0.0 0.0 0 0.00
90.6
171.6
19286
49.87
2.2 4.2 471
1.21
1.9 3.7 413
1.07
1.4 2.7 308
0.77
0.2 0.3
35
0.08
3.6 6.9 773
1.86
0.0 0.0 0 0.00
total I 620844 1
| 618775 | 100 | 189 | 21285 | 54.9 |
cholestane j 78519 | 1.04 |
81660 81660
|
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A93 of A172
Appendix to TNO-report of 3M-02
Final version
3:4-10 weight feces
6.8
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 434209
0.98
425525
90.4 89.6 13179 34.08
cholestanol 12431
0.91
11312
2.4 2.4 350
0.90
lathosterol
9196
1
9196
2.0 1.9 285 0.74
campesterol 4448
1.56
6939
1.5 1.5 215 0.54
stigmasterol
651
1.49
970
0.2 0.2
30
0.07
b-sitosterol 11421
1.47
16789
3.6 3.5 520
1.25
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 472356 I
| 470731 | 100 | 99 | 14579 | 37.6 |
cholestane | TT4T42 | 104 |
3:4-11 weight feces
6.8
mg
118708 118708
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol
690765
1 1 0.98
0 0 676950
0.0 0.0 0 0.00 0.0 0.0 0 0.00 91.4 122.7 18042 46.66
cholestanol lathosterol
15497 14645
0.91 1
14102 14645
1.9 2.6 376 2.0 2.7 390
0.97 1.01
campesterol stigmasterol b-sitosterol b-sitostanol
6317 805 16234
1.56 1.49 1.47
1
9855 1199 23864
0
1.3 1.8 263
0.2 0.2
32
3.2 4.3 636
0.0 0.0
0
0.66 0.08 1.53 0.00
total I 744263 |
|
740615
| 100 | 134 | 19739 | 50.9 |
cholestane | 132638 | 104 I
3:4-12 weight feces
7.7
mg
137944 137944
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
657017 16315 13781 6401 812 16291
1 1 0.98 0.91 1 1.56 1.49 1.47 1
0 0 643877 14847 13781 9986 1210 23948 0
0.0 0.0 0 0.00
0.0 0.0 0 0.00
91.0 121.8 15814 40.89
2.1 2.8 365 0.94
1.9 2.6 338
0.88
1.4 1.9 245 0.61
0.2 0.2
30
0.07
3.4 4.5 588
1.42
0.0 0.0 0 0.00
total I 710617 |
I 707648 | 100 | 134 | 17380 | 44.8 |
cholestane | 12710^ | 1.04 |
132193 132193
|
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A94 of A172
Appendix to TNO-report of 3M-02
Final version
| 1:5-13 weight feces
7.8
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol
b-sitosterol b-sitostanol
area
482166 15148 13001 5131 1060 14512
response area corrected factor for response factor
10 10 0.98 472523 0.91 13785 1 13001 1.56 8004 1.49 1579
1.47 21333 10
%
0.0 0.0 89.1 2.6 2.5 1.5 0.3 4.0 0.0
pg sterol
0.0 0.0 129.2 3.8 3.6 2.2 0.4 5.8 0.0
ng sterol/ mg feces
0 0 16561 483 456 281 55
748 0
nmol sterol/ mg feces
0.00 0.00 42.83 1.24 1.18 0.70 0.13
1.80 0.00
total | 531018 |
I 530225 | 100 | 145 | 18583 | 47.9 |
cholestane | 87934 | 104 |
| 1:5-14 weight feces
13.4 mg
9T45T 91451
'
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0 0.0
0
0.00
coprostanon
1
0
0.0 0.0
0
0.00
cholesterol cholestanol lathosterol
634279 12599 16167
0.98 0.91
1
621593 11465 16167
89.9
187.2
13972
36.13
1.7 3.5 258
0.66
2.3 4.9 363
0.94
campesterol 6696
1.56
10446
1.5 3.1 235 0.59
stigmasterol b-sitosterol b-sitostanol
2927 18799
1.49 1.47
1
4361 27635
0
0.6 1.3
98
4.0 8.3 621
0.0 0.0
0
0.24 1.50 0.00
total | 691467 |
| 691667 | 100 | 208 | 15547 | 40.1 |
cholestane | 7981 | 1.04 |
83003
____________________________________ 83003
1:5-15
weight feces
6.2
mg
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0 0.0
0
0.00
coprostanon
1
0
0.0 0.0
0
0.00
cholesterol 255399
0.98
250291
85.5 66.1 10669 27.59
cholestanol 13187
0.91
12000
4.1 3.2 512
1.32
lathosterol
6307
1
6307
2.2 1.7 269 0.70
campesterol 3843
1.56
5995
2.0 1.6 256 0.64
stigmasterol
729
1.49
1086
0.4 0.3
46
0.11
b-sitosterol 11657
1.47
17136
5.9 4.5 730
1.76
b-sitostanol
1
0
0.0 0.0
0
0.00
total | 291122 |
| 292815 | 100 | 77 | 12481 | 32.1 |
cholestane 90959
1.04
94597 94597
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A95 of A172
Appendix to TNO-report of 3M-02
Final version
| 2:5-13
weightfeces 7.9
mg
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 455921 0.98
446803
89.0 124.6 15774 40.79
cholestanol 13655 0.91 12426 2.5 3.5 439 1.13
lathosterol 12928 1 12928 2.6 3.6 456 1.18
campesterol 5203 1.56
8117
1.6 2.3 287 0.72
stigmasterol 898 1.49
1338
0.3 0.4 47 0.11
b-sitosterol 13941 1.47
20493
4.1 5.7 723 1.74
b-sitostanol
1 0 0.0 0.0 0 0.00
total | 502546 |
| 502105 | 100 | 140 | 17726 | 45.7 |
cholestane | 86190 | 104 |
| 2:5-14
weightfeces 6.9
mg
89638 89638
|
|
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 302926 0.98
296867
90.2 101.4 14697 38.01
cholestanol 6271 0.91
5707
1.7 1.9 283 0.73
lathosterol 8314 1 8314 2.5 2.8 412 1.06
campesterol 3149 1.56
4912
1.5 1.7 243 0.61
stigmasterol
5
LO
1.49 806
0.2 0.3 40 0.10
b-sitosterol 8475 1.47
12458
3.8 4.3 617 1.49
b-sitostanol
1 0 0.0 0.0 0 0.00
total | 329676 |
| 329065 | 100 | 112 | 16291 | 42.0 |
cholestane | 70369 | 1.04 |
| 2:5-15
weightfeces 4.8
mg
73184 73184
|
|
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 210645 0.98
206432
83.6 52.2 10870 28.11
cholestanol 13493 0.91 12279 5.0 3.1 647 1.66
lathosterol 5461
1
5461
2.2 1.4 288 0.74
campesterol 3832 1.56
5978
2.4 1.5 315 0.79
stigmasterol 733 1.49
1092
0.4 0.3 58 0.14
b-sitosterol 10573 1.47
15542
6.3 3.9 818 1.97
b-sitostanol
1 0 0.0 0.0 0 0.00
total | 244737 |
| 246784 | 100 | 62 | 12995 | 33.4 |
cholestane 95106 1.04
98910 98910
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A96 of A172
Appendix to TNO-report of 3M-02
Final version
| 3:5-13 weight feces
11.8
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon
1 0 0.0 0.0 0 0.00 1 0 0.0 0.0 0 0.00
cholesterol cholestanol
760655 21051
0.98 0.91
745442 19156
88.7
146.6
12425
32.13
2.3 3.8 319 0.82
lathosterol
19855
1
19855
2.4 3.9 331
0.86
campesterol 9056
1.56
14127
1.7 2.8 235 0.59
stigmasterol 3585
1.49
5342
0.6 1.1 89 0.22
b-sitosterol b-sitostanol
24867
1.47 1
36554 0
4.3 7.2 609
1.47
0.0 0.0
0
0.00
total I 839069 |
| 840477 | 100 | 165 | 14009 | 36.1 ~ |
cholestane | 12221^ | 4 |
127107
|
_____________________________________ 127107_____________________________________________
| 3:5-14
~
weight feces
7.2
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon cholesterol cholestanol
475539 8894
1 0.98 0.91
0 466028
8094
0.0 0.0 0 0.00
90.3
88.3
12257
31.70
1.6 1.5 213 0.55
lathosterol campesterol stigmasterol
11319 4936 2133
1 1.56 1.49
11319 7700 3178
2.2 2.1 298 0.77 1.5 1.5 203 0.51 0.6 0.6 84 0.20
b-sitosterol b-sitostanol
13417
1.47 1
19723 0
3.8 3.7 519
1.25
0.0 0.0
0
0.00
total I 516238 |
| 516042
| 100 | 98 | 13573 | 35.0 ~ |
cholestane | 126938 | "
|
| 3:5-15 weight feces
7.1
mg
132016 132016
|
~
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon cholesterol
369324
1 1 0.98
0 0 361938
0.0 0.0 0 0.00 0.0 0.0 0 0.00 85.4 62.2 8763 22.66
cholestanol lathosterol campesterol
21096 8285 6116
0.91 1
1.56
19197 8285 9541
4.5 3.3 465
1.20
2.0 1.4 201
0.52
2.3 1.6 231
0.58
stigmasterol b-sitosterol b-sitostanol
1212 15819
1.49 1.47
1
1806 23254
0
0.4 0.3 44 0.11
5.5 4.0 563
1.36
0.0 0.0
0
0.00
total I 421852 |
| 424021
| 100 | 73 | 10267 | 26.4 ~ |
cholestane | 139834 | 1.04 |
145427 145427
|
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A97 of A172
Appendix to TNO-report of 3M-02
Final version
1:6-16
weightfeces 5.5
mg
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 316760 0.98
310425
88.4 75.3 13682 35.38
cholestanol 10679 0.91
9718
2.8 2.4 428 1.10
lathosterol 7883 1 7883 2.2 1.9 347 0.90
campesterol 3863 1.56
6026
1.7 1.5 266 0.66
stigmasterol 573 1.49
854
0.2 0.2 38 0.09
b-sitosterol 10951 1.47
16098
4.6 3.9 710 1.71
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 350709 1
| 351004 | 100 | 85 | 15471 | 39.8 |
cholestane | 99T6 | 104 |
1:6-17
weightfeces 5.1
mg
103127 103127
|
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 283717 0.98
278043
86.7 61.2 12006 31.05
cholestanol 12638 0.91
11501
3.6 2.5 497 1.28
lathosterol 7544 1 7544 2.4 1.7 326 0.84
campesterol 4033 1.56
6291
2.0 1.4 272 0.68
stigmasterol 593 1.49
884
0.3 0.2 38 0.09
b-sitosterol 11162 1.47
16408
5.1 3.6 709 1.71
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 319687 1
| 320670 | 100 | 71 | 13847 | 35.6 |
cholestane | 109156 | 104 |
1:6-18
weightfeces 9.3
mg
113522 113522
|
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 432750 0.98
424095
87.8 121.3 13048 33.74
cholestanol 15694 0.91
14282
3.0 4.1 439 1.13
lathosterol 11216 1
11216
2.3 3.2 345 0.89
campesterol 5531 1.56
8628
1.8 2.5 265 0.66
stigmasterol 828 1.49
1234
0.3 0.4 38 0.09
b-sitosterol 15866 1.47
23323
4.8 6.7 718 1.73
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 481885 1
| 482778 | 100 | 138 | 14853 | 38.2 |
cholestane 84015 1.04
87376 87376
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A98 of A172
Appendix to TNO-report of 3M-02
Final version
2:6-16 weight feces
5.2
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
306320 10414 7025 3400
583 9578
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 300194 9477 7025 5304 869 14080 0
%
0.0 0.0 89.1 2.8 2.1 1.6 0.3 4.2 0.0
|jg sterol
0.0 0.0 71.3 2.2 1.7 1.3 0.2 3.3 0.0
ng sterol/ mg feces
0 0 13705 433 321 242 40 643 0
nmol sterol/ mg feces
0.00 0.00 35.44 1.11 0.83 0.60 0.10 1.55 0.00
total I 337320 1
| 336948 | 100 | 80 | 15383 | 39.6 |
cholestane | 10125^ | 1.04 |
2:6-17 weight feces
4.6
mg
105306 105306
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
264813 13978 6351 4076
392 11081
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 259517 12720 6351 6359 584 16289 0
%
0.0 0.0 86.0 4.2 2.1 2.1 0.2 5.4 0.0
jg sterol
0.0 0.0 58.0 2.8 1.4 1.4 0.1 3.6 0.0
ng sterol/ mg feces
0 0 12612 618 309 309 28 792 0
nmol sterol/ mg feces
0.00 0.00 32.61 1.59 0.80 0.77 0.07 1.91 0.00
total I 300691 1
| 301819 | 100 | 67 | 14667 | 37.8 |
cholestane | 107534 | 1.04 |
2:6-18 weight feces
7.1
mg
111835 111835
|
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
415098 15248 11257 5140
790 14501
response factor
1 1 0.98 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 406796 13876 11257 8018 1177 21316 0
%
0.0 0.0 88.0 3.0 2.4 1.7 0.3 4.6 0.0
jg sterol
0.0 0.0 89.1 3.0 2.5 1.8 0.3 4.7 0.0
ng sterol/ mg feces
0 0 12553 428 347 247 36 658 0
nmol sterol/ mg feces
0.00 0.00 32.46 1.10 0.90 0.62 0.09 1.59 0.00
total
I 462034 1
| 462441
| 100 | 101 | 14270 | 36.8 |
cholestane | 109718 | 1.04 |
114107 114107
|
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A99 of A172
Appendix to TNO-report of 3M-02
Final version
3:6-16
weightfeces 9.4
mg
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 528030 0.98
517469
90.2 120.9 12861 33.26
cholestanol 15892 0.91
14462
2.5 3.4 359 0.92
lathosterol 10925 1
10925
1.9 2.6 272 0.70
campesterol 5503 1.56
8585
1.5 2.0 213 0.53
stigmasterol
ID
1.49 1134
0.2 0.3 28 0.07
b-sitosterol 14410 1.47
21183
3.7 4.9 526 1.27
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 575521 1
| 573757 | 100 | 134 | 14260 | 36.8 |
cholestane | 102895 | " |
3:6-17
weightfeces 5.2
mg
107011 107011
|
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 325132 0.98
318629
87.3 55.5 10671 27.59
cholestanol 15757 0.91
14339
3.9 2.5 480 1.24
lathosterol 7236 1 7236 2.0 1.3 242 0.63
campesterol 4495 1.56
7012
1.9 1.2 235 0.59
stigmasterol 611 1.49
910
0.2 0.2 30 0.07
b-sitosterol 11479 1.47
16874
4.6 2.9 565 1.36
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 36471^1
| 365001 | 100 | 64 | 12224 | 31.5 |
cholestane | 138038 | 4 |
3:6-18
weightfeces 6.9
mg
143560 | 143560
area response areacorrected % pgsterol ngsterol/ nmol sterol/
factor forresponsefactor
mgfeces mgfeces
coprostanol
1 0 0.0 0.0 0 0.00
coprostanon
1 0 0.0 0.0 0 0.00
cholesterol 716301 0.98
701975
88.7 96.4 13976 36.14
cholestanol 24577 0.91
22365
2.8 3.1 445 1.15
lathosterol 18733 1
18733
2.4 2.6 373 0.96
campesterol 8508 1.56
13272
1.7 1.8 264 0.66
stigmasterol 1102 1.49
1642
0.2 0.2 33 0.08
b-sitosterol 22755 1.47
33450
4.2 4.6 666 1.61
b-sitostanol
1 0 0.0 0.0 0 0.00
total I 791976 1
| 791437 | 100 | 109 | 15758 | 40.6 |
cholestane 174978 1.04
181977 181977
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A100 of A172
Appendix to TNO-report of 3M-02
Final version
| 1:7-19 weight feces
7 .5
mg
coprostanol coprostanon cholesterol cholestanol
lathosterol cam pestero l stig m a s te ro l b-sitosterol b-sitostanol
area
449806 25289 7667 6477
917 18151
response factor
1 1 0 .9 8 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 440810 23013 7667 10104 1366 26682 0
%
0.0 0.0 86.5 4.5 1.5 2.0 0.3 5.2 0.0
pg sterol
0 .0 0 .0 98.4 5.1 1.7 2.3 0.3 6 .0 0.0
ng sterol/ mg feces
0 0 13115 685 228 301 41 794 0
nmol sterol/ mg feces
0 .0 0 0 .0 0 33.92 1.76 0 .5 9 0 .7 5 0 .1 0 1.91 0 .0 0
total
I 508307 |
| 509642
| 100 | 114 | 15163 | 39.0 ~ |
cholestane | 107726 | 1 0 4 |
| 1 :7-20 weight feces
10.8
mg
112035 112035
|
'
coprostanol coprostanon cholesterol cholestanol
lathosterol c am pestero l stig m a s te ro l b-sitosterol b-sitostanol
area
497481 30777 9362 7714 1041 21441
response factor
1 1 0 .9 8 0.91 1 1.56 1.49 1.47 1
area corrected for response factor
0 0 487531 28007 9362 12034 1551 31518 0
%
0.0 0.0 85.5 4.9 1.6 2.1 0.3 5.5 0.0
pg sterol
0 .0 0 .0 119.2 6 .9 2 .3 2 .9 0 .4 7.7 0.0
ng sterol/ mg feces
0 0 11041 634 212 273 35 714 0
nmol sterol/ mg feces
0 .0 0 0 .0 0 28.55 1.63 0 .5 5 0 .6 8 0 .0 9 1.72 0 .0 0
total
I 567816 |
|
570004
| 100 | 139 | 12909 |
33.2 ~ |
cholestane | 98281 | "
|
| 1:7-21 weight feces
8 .5
mg
102212 102212
|
1
coprostanol coprostanon cholesterol cholestanol
lathosterol c am pestero l stig m a s te ro l b-sitosterol b-sitostanol
area
328489 18232 5881 5028
654 13624
response area corrected factor for response factor
1 1 0 .9 8 0.91 1 1.56 1.49 1.47 1
0 0 321919 16591 5881 7844 974 20027 0
%
0.0 0.0 86.3 4.4 1.6 2.1 0.3 5.4 0.0
pg sterol
0 .0 0 .0 100.2 5.2 1.8 2 .4 0 .3 6.2 0.0
ng sterol/ mg feces
0 0 11792 608 215 287 36 734 0
nmol sterol/ mg feces
0 .0 0 0 .0 0 30.49 1.56 0 .5 6 0 .7 2 0 .0 9 1.77 0 .0 0
total
I 371908 |
|
373237
| 100 | 116 | 13671 |
35.2 ~ |
cholestane
77207
1.04
80295 80295
coprostanol coprostanon cholesterol cholestanol
lathosterol c am pestero l stig m a s te ro l b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A101 of A172
Appendix to TNO-report of 3M-02
Final version
weight feces
7.3
mg
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
393028 19805 6961 5235 2749 13255
response area corrected factor for response factor
10 10 0.98 385167 0.91 18023 1 6961 1.56 8167 1.49 4096 1.47 19485 10
%
0.0 0.0 87.2 4.1 1.6 1.8 0.9 4.4 0.0
pg sterol
0.0 0.0 87.9 4.1 1.6 1.9 0.9 4.4 0.0
ng sterol/ mg feces
0 0 12046 564 218 255 128 609 0
nmol sterol/ mg feces
0.00 0.00 31.15 1.45 0.56 0.64 0.31 1.47 0.00
total | 441033 J
| 441898 | 100 | 101 | 13820 | 35.6 |
cholestane I 105292 | 1.04 |
| 2:7-20 weight feces
7
mg
109504 109504
|
|
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
363884 22163 6943 5485
752 14149
response area corrected factor for response factor
10 10 0.98 356606 0.91 20168 1 6943 1.56 8557 1.49 1120 1.47 20799 10
%
0.0 0.0 86.1 4.9 1.7 2.1 0.3 5.0 0.0
pg sterol
0.0 0.0 91.7 5.2 1.8 2.2 0.3 5.4 0.0
ng sterol/ mg feces
0 0 13105 741 255 314 41 764 0
nmol sterol/ mg feces
0.00 0.00 33.89 1.91 0.66 0.78 0.10 1.84 0.00
total I 413376 J
| 414194 | 100 | 107 | 15222 | 39.2 |
cholestane | 93444 | 1.04 |
| 2:7-21 weight feces
6.9
mg
97182 97182
|
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon
1 0 0.0 0.0 0 0.00 1 0 0.0 0.0 0 0.00
cholesterol cholestanol lathosterol
399924 19224 7234
0.98 0.91
1
391926 17494 7234
87.3
88.6
12845
33.22
3.9 4.0 573
1.48
1.6 1.6 237 0.61
campesterol stigmasterol
5395 2024
1.56 1.49
8416 3016
1.9 1.9 276
0.7 0.7
99
0.69 0.24
cO)o
b-sitosterol b-sitostanol
14143
1.47 1
20790 0
4.6 4.7
1.64
0.0 0.0 0 0.00
total | 447944 1
| 448876 | 100 | 102 | 1471^ | 37.9 |
cholestane j 106300 | 1.04 |
110552 110552
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A102 of A172
Appendix to TNO-report of 3M-02
Final version
| 3:7-19 weight feces
10
mg
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon
1 0 0.0 0.0 0 0.00 1 0 0.0 0.0 0 0.00
cholesterol cholestanol lathosterol
582689 23901 10390
0.98 0.91
1
571035 21750 10390
88.5
114.6
11457
29.63
3.4 4.4 436
1.12
1.6 2.1 208 0.54
campesterol 7529
1.56
11745
1.8 2.4 236
0.59
stigmasterol
942
1.49
1404
0.2 0.3 28 0.07
b-sitosterol b-sitostanol
19466
1.47 1
28615 0
4.4 5.7 574
0.0 0.0
0
1.38 0.00
total | 644917 |
| 644939
| 100 | 129 | 12940 | 33.3 |
cholestane | 11981^ | 1.04 |
| 3:7-20 weight feces
4.9
mg
124603 124603
|
|
coprostanol coprostanon cholesterol cholestanol
lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
area
282329 16221 5485 3642 437 9568
response area corrected factor for response factor
10 10 0.98 276682 0.91 14761 1 5485 1.56 5682 1.49 651 1.47 14065 10
%
0.0 0.0 87.2 4.7 1.7 1.8 0.2 4.4 0.0
pg sterol
0.0 0.0 56.9 3.0 1.1 1.2 0.1 2.9 0.0
ng sterol/ mg feces
0 0 11609 619 230 238 27 590 0
nmol sterol/ mg feces
0.00 0.00 30.02 1.59 0.60 0.59 0.07 1.42 0.00
total | 317682 l
| 317326 | 100 | 65 | 13314 | 34.3 |
cholestane | 116924 | 14 |
| 3:7-21 weight feces
9.5
mg
121601 121601
|
|
area response area corrected
% pg sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol coprostanon
1 0 0.0 0.0 0 0.00 1 0 0.0 0.0 0 0.00
cholesterol 486322
0.98
476596
88.6
110.7
11653
30.13
cholestanol lathosterol
21163 7823
0.91 1
19258 7823
3.6 4.5 471 1.5 1.8 191
1.21 0.49
campesterol stigmasterol
6293 622
1.56 1.49
9817 927
1.8 2.3 240
0.2 0.2
23
0.60 0.05
b-sitosterol b-sitostanol
16081
1.47 1
23639 0
4.4 5.5 578
0.0 0.0
0
1.39 0.00
total | 538304 |
| 538060 | 100 | 125 | 1315^ | 33.9 |
cholestane 103492
1.04
107632 107632
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A103 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A104 of A172
group
1 1 1 1
i 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 AVERAGE SD
cage#
1,2 3 ,4
5 1,2 3 ,4 5 1,2 3 ,4 5
GC sam ple#
1:1-1 1 :1 -2 1 :1 -3 2:1-1 2 :1 -2 2 :1 -3 3:1-1 3 :1 -2 3 :1 -3
coprostanol 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
neutral sterols (um ol/g feces) coprostanon cholesterol cholestanol
0 .0 0
2 5 .1 3
0 .7 5
0 .0 0
3 0 .5 2
0 .8 4
0 .0 0
2 6 .7 6
0 .7 1
0 .0 0
3 2 .4 1
1 .0 7
0 .0 0 0 .0 0
3 2 .5 6 3 4 .1 7
0 .8 9 0 .9 3
0 .0 0
3 1 .3 4
0 .8 8
0 .0 0
2 7 .1 0
0 .7 8
0 .0 0
2 8 .0 3
0 .7 1
lath osterol 0.91 0 .8 9 0 .9 4 1 .0 8 0 .8 5 1 .1 4 0 .8 6 0 .7 5 0 .8 0
neutral sterols total (um ol/g feces)
2 6 .7 9 3 2 .2 6 2 8 .4 0 3 4 .5 6 3 4 .3 0 3 6 .2 5 3 3 .0 8 2 8 .6 4 2 9 .5 4
total feces
(g) 3 .0 7 5 2.4 8 9 7 2.2 9 4 6 4 .6 9 2 3.7 7 8 9 3.4 1 0 6 3.5 7 5 4 2 .8 7 8 1 2.7 5 8 9
average w eight
(g) 1 2 3 .6 8 7 .2 5 8 8 .5 5 1 2 2 .4 5 8 6 .5 5 8 8 .2 1 2 1 .6 5 8 6 .5 5 8 8 .0 5
days
2 2 2 3 3 3 2 2 2
6 7 ,8 9 ,1 0
6 7 ,8 9 ,1 0
6 7 ,8 9 ,1 0
1 :2 -4 1 :2 -5 1 :2 -6 2 :2 -4 2 :2 -5 2 :2 -6 3 :2 -4 3 :2 -5 3 :2 -6
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
3 5 .1 2 4 0 .9 8 2 7 .1 6 3 5 .5 6 3 9 .7 5 3 1 .1 4 3 0 .7 6 2 9 .9 5 2 8 .9 8
0 .6 7 0 .5 2 0 .9 7 0 .8 0 0 .7 5 1 .0 9 0 .7 5 0 .5 5 0 .9 9
0 .9 7 1 .3 3 0 .7 9 0 .9 8 1 .2 4 0 .8 0 0 .8 2 0 .8 8 0 .7 0
3 6 .7 5 4 2 .8 3 2 8 .9 2 3 7 .3 3 4 1 .7 4 3 3 .0 4 3 2 .3 4 3 1 .3 8 3 0 .6 6
2.7 6 1 9 4 .0 5 8 1 2.0 9 3 5 4 .0 7 5 6 .3 7 1 3.4 0 7 4 2.9 2 7 9 4.3 9 8 5 2.4 5 7 5
9 4 .5 5 1 2 6 .6 8 2 .2 9 4 .2 5 1 2 6 .4 5 82.1
94 1 2 6 .6 8 1 .8 5
2 2 2 3 3 3 2 2 2
1 1 ,1 2 ,1 3 15
1 4 ,1 6 ,1 7
1 1 ,1 2 ,1 3 15
1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7
1 :3 -7 1 :3 -8 1 :3 -9
2 :3 -7 2 :3 -8 2 :3 -9 3 :3 -7 3 :3 -8 3 :3 -9
0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
2 9 .0 4 2 6 .4 9 3 6 .7 3
3 0 .7 1 3 1 .8 6 3 6 .7 5 2 9 .9 9 2 5 .4 1 3 0 .7 1
0 .8 1 0 .9 5 0 .9 0
0 .8 0 0 .8 9 0 .9 4 0 .7 9 0 .8 6 0 .8 3
1 .1 4 0 .7 9 0 .8 3
1.11 0 .9 4 0 .8 3 0 .9 6 0 .6 5 0.71
3 0 .9 9 2 8 .2 3 3 8 .4 6
3 2 .6 2 3 3 .7 0 3 8 .5 2 3 1 .7 4 2 6 .9 3 3 2 .2 5
3.4 5 9 7 2.2 9 9 4 2.3 4 5 4
5.8 1 4 7 3.7 7 1 8 3.7 7 2 7 4 .0 2 5 2.5 2 6 5 2.6 9 0 5
118 8 3 .5 5 8 2 .8
1 1 8 .7 5 8 3 .5 8 3 .0 5 1 1 9 .6 8 4 .2 8 3 .4 5
2 2 2
3 3 3 2 2 2
18 19 2 0 ,2 1 18 19 2 0 ,2 1 18 19 2 0 ,2 1
1 :4 -1 0 1 :4 -1 1 1 :4 -1 2 2 :4 -1 0 2:4-11 2 :4 -1 2 3 :4 -1 0 3:4-11 3 :4 -1 2
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
3 5 .7 6 3 4 .6 0 4 1 .0 1 4 7 .7 7 4 8 .0 1 4 9 .8 7 3 4 .0 8 4 6 .6 6 4 0 .8 9
0 .8 6 0 .9 8 1 .2 4 1 .2 6 1 .1 9 1.21 0 .9 0 0 .9 7 0 .9 4
0 .8 0 0 .7 8 1 .0 2 1 .0 3 1 .0 0 1 .0 7 0 .7 4 1.01 0 .8 8
3 7 .4 1 3 6 .3 7 4 3 .2 7 5 0 .0 7 5 0 .2 0 5 2 .1 5 3 5 .7 2 4 8 .6 3 4 2 .7 1
1 .6 8 9 3.1 4 3 2 3.2 5 4 5 2.7 1 3 3 4.6 4 0 7 5.2 0 7 5 2 .0 4 1 3.4 4 6 4 3.9 5 4 9
5 9 .5 1 1 6 .4 1 1 2 .2 5 8 .8 5 1 1 6 .5 5 1 1 1 .8 5 5 8 .7 1 1 7 .4 5 1 1 2 .9
2 2 2 3 3 3 2 2 2
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
1 :5 -1 3 1 :5 -1 4 1 :5 -1 5 2 :5 -1 3 2 :5 -1 4 2 :5 -1 5 3 :5 -1 3 3 :5 -1 4 3 :5 -1 5
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
4 2 .8 3 3 6 .1 3 2 7 .5 9 4 0 .7 9 3 8 .0 1 2 8 .1 1 3 2 .1 3 3 1 .7 0 2 2 .6 6
1 .2 4 0 .6 6 1 .3 2 1 .1 3 0 .7 3 1 .6 6 0 .8 2 0 .5 5 1 .2 0
1 .1 8 0 .9 4 0 .7 0 1 .1 8 1 .0 6 0 .7 4 0 .8 6 0 .7 7 0 .5 2
4 5 .2 5 3 7 .7 3 2 9 .6 0 4 3 .1 0 3 9 .8 0 3 0 .5 2 3 3 .8 1 3 3 .0 1 2 4 .3 8
3 .1 9 5 2.9 4 0 8 2.6 3 9 9 4.9 7 1 2 4.4 8 3 3 3 .7 6 7 3.6 1 1 3 3.3 5 2 2 2.7 0 3 4
1 1 0 .7 8 8 .3 5 8 4 .6 1 1 1 .6 8 8 .5 8 5 .5 5 1 1 2 .8 5 8 8 .9 8 5 .5
2 2 2 3 3 3 2 2 2
2 8 ,2 9 31
3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2
1 :6 -1 6 1 :6 -1 7 1 :6 -1 8 2 :6 -1 6 2 :6 -1 7 2 :6 -1 8 3 :6 -1 6 3 :6 -1 7 3 :6 -1 8
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
3 5 .3 8 3 1 .0 5 3 3 .7 4 3 5 .4 4 3 2 .6 1 3 2 .4 6 3 3 .2 6 2 7 .5 9 3 6 .1 4
1 .1 0 1 .2 8 1 .1 3 1.11 1 .5 9 1 .1 0 0 .9 2 1 .2 4 1 .1 5
0 .9 0 0 .8 4 0 .8 9 0 .8 3 0 .8 0 0 .9 0 0 .7 0 0 .6 3 0 .9 6
3 7 .3 8 3 3 .1 7 3 5 .7 6 3 7 .3 8 3 5 .0 0 3 4 .4 6 3 4 .8 8 2 9 .4 6 3 8 .2 5
2.5 0 3 6 2.7 0 8 3 2.3 5 5 4 3.9 6 8 4 3.9 6 7 8 3.5 6 6 6 2.9 0 7 6 3.2 4 4 9 2.7 6 4 3
87.1 1 1 2 .8 8 5 .4 8 7 .1 5 1 1 2 .4 5 8 4 .5 5 8 7 .6 5 1 1 1 .9 5 8 4 .6 5
2 2 2 3 3 3 2 2 2
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
1 :7 -1 9 1 :7 -2 0 1 :7 -2 1 2 :7 -1 9 2 :7 -2 0 2:7-21 3 :7 -1 9 3 :7 -2 0 3:7-21
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
3 3 .9 2 2 8 .5 5 3 0 .4 9 3 1 .1 5 3 3 .8 9 3 3 .2 2 2 9 .6 3 3 0 .0 2 3 0 .1 3
1 .7 6 1 .6 3 1 .5 6 1 .4 5 1.91 1 .4 8 1 .1 2 1 .5 9 1.21
0 .5 9 0 .5 5 0 .5 6 0 .5 6 0 .6 6 0.61 0 .5 4 0 .6 0 0 .4 9
3 6 .2 7 3 0 .7 3 3 2 .6 1 3 3 .1 6 3 6 .4 6 3 5 .3 0 3 1 .2 9 3 2 .2 1 3 1 .8 4
1 .9 6 9 7 2.0 7 9 8 2.6 7 0 5 3.4 4 7 7 3.2 6 4 6 4.3 0 5 9 2.5 1 1 7 2.4 3 6 9 3.4 1 0 7
8 1 .3 5 8 2 .2 5 1 0 1 .0 5 8 2 .3 5 8 2 .7 5 1 0 1 .9 5 8 3 .5 8 3 .3 5 1 0 3 .2
2 2 2 3 3 3 2 2 2
total neutrol sterols excretion (um ol/100 gr m ouse/day) 3 3 .3 3 4 6 .0 2 3 6 .8 0 4 4 .1 4 4 9 .9 2 4 6 .7 3 4 8 .6 2 4 7 .6 2 4 6 .2 8 4 4 .3 8 5 .6 0 5 3 .6 7 6 8 .6 4 3 6 .8 3 5 3 .8 0 7 0 .1 0 4 5 .7 0 5 0 .3 6 5 4 .5 1 4 6 .0 3 5 3 .2 9 1 0 .6 6 4 5 .4 3 3 8 .8 5 5 4 .4 8 5 3 .2 4 5 0 .7 4 5 8 .3 3 5 3 .4 0 4 0 .4 0 5 1 .9 9 4 9 .6 5 6 .6 4 5 3 .1 0 4 9 .1 0 6 2 .7 5 7 6 .9 5 6 6 .6 3 8 0 .9 4 6 2 .1 0 7 1 .3 5 7 4 .8 0 6 6 .4 1 1 0 .7 6 6 5 .3 0 6 2 .8 0 4 6 .1 8 6 4 .0 0 6 7 .2 0 4 4 .7 9 5 4 .1 0 6 2 .2 5 3 8 .5 4 5 6 .1 3 1 0 .5 6 5 3 .7 3 3 9 .8 2 4 9 .3 2 5 6 .7 4 4 1 .1 7 4 8 .4 6 5 7 .8 6 4 2 .6 9 6 2 .4 6 5 0 .2 5 8.01 4 3 .9 1 3 8 .8 6 4 3 .1 0 4 6 .2 8 4 7 .9 4 4 9 .7 0 4 7 .0 6 4 7 .0 8 5 2 .6 1 4 6 .2 8 3 .9 8
coprostanol 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
% o f neutral sterols coprostanon cholesterol cholestanol
0 .0 0
9 3 .7 9
2 .8 1
0 .0 0 0 .0 0
9 4 .6 2 9 4 .2 1
2 .6 0 2 .4 9
0 .0 0
9 3 .7 7
3 .1 0
0 .0 0 0 .0 0
9 4 .9 3 9 4 .2 7
2 .5 9 2 .5 7
0 .0 0
9 4 .7 4
2 .6 7
0 .0 0
9 4 .6 4
2 .7 4
0 .0 0
9 4 .8 7
2 .4 1
0 .0 0 0 .0 0
9 4 .4 3 0 .4 4
2 .6 7 0 .2 0
0 .0 0
9 5 .5 6
1.81
0 .0 0 0 .0 0
9 5 .6 8 9 3 .9 2
1.21 3 .3 7
0 .0 0
9 5 .2 5
2 .1 4
0 .0 0 0 .0 0
9 5 .2 5 9 4 .2 7
1 .7 9 3 .3 0
0 .0 0
9 5 .1 3
2 .3 3
0 .0 0 0 .0 0
9 5 .4 4 9 4 .5 0
1 .7 5 3 .2 2
0 .0 0
9 5 .0 0
2 .3 2
0 .0 0
0 .6 2
0 .7 9
0 .0 0
9 3 .7 0
2 .6 1
0 .0 0 0 .0 0
9 3 .8 5 9 5 .5 0
3 .3 5 2 .3 4
0 .0 0
9 4 .1 4
2 .4 7
0 .0 0 0 .0 0
9 4 .5 6 9 5 .4 0
2 .6 4 2 .4 3
0 .0 0
9 4 .5 1
2 .4 8
0 .0 0 0 .0 0
9 4 .3 9 9 5 .2 3
3 .1 9 2 .5 7
0 .0 0 0 .0 0
9 4 .5 9 0 .6 6
2 .6 8 0 .3 5
0 .0 0 0 .0 0
9 5 .5 8 9 5 .1 4
2 .2 9 2 .7 0
0 .0 0
9 4 .7 8
2 .8 7
0 .0 0
9 5 .4 1
2 .5 2
0 .0 0 0 .0 0
9 5 .6 4 9 5 .6 3
2 .3 7 2 .3 2
0 .0 0
9 5 .4 1
2 .5 2
0 .0 0 0 .0 0
9 5 .9 4 9 5 .7 5
1 .9 9 2 .2 0
0 .0 0
9 5 .4 8
2 .4 2
0 .0 0
0 .3 5
0 .2 7
0 .0 0 0 .0 0 0 .0 0
9 4 .6 5 9 5 .7 5 9 3 .2 1
2 .7 5 1 .7 6 4 .4 5
0 .0 0 0 .0 0
9 4 .6 4 9 5 .5 0
2 .6 2 1 .8 3
0 .0 0
9 2 .1 1
5 .4 5
0 .0 0
9 5 .0 4
2 .4 3
0 .0 0 0 .0 0
9 6 .0 1 9 2 .9 7
1 .6 6 4 .9 1
0 .0 0
9 4 .4 3
3 .0 9
0 .0 0
1 .3 6
1 .4 5
0 .0 0 0 .0 0
9 4 .6 5 9 3 .6 1
2 .9 5 3 .8 5
0 .0 0
9 4 .3 4
3 .1 6
0 .0 0 0 .0 0 0 .0 0
9 4 .8 0 9 3 .1 8 9 4 .2 0
2 .9 8 4 .5 4 3 .2 0
0 .0 0 0 .0 0
9 5 .3 4 9 3 .6 8
2 .6 5 4 .1 9
0 .0 0
9 4 .4 8
2 .9 9
0 .0 0 0 .0 0
9 4 .2 5 0 .6 7
3 .3 9 0 .6 5
0 .0 0
9 3 .5 2
4 .8 6
0 .0 0
9 2 .9 1
5 .3 1
0 .0 0
9 3 .5 0
4 .7 9
0 .0 0 0 .0 0 0 .0 0
9 3 .9 3 9 2 .9 6 9 4 .0 9
4 .3 7 5 .2 3 4 .1 8
0 .0 0 0 .0 0
9 4 .6 9 9 3 .2 1
3 .5 9 4 .9 5
0 .0 0
9 4 .6 4
3 .8 0
0 .0 0 0 .0 0
9 3 .7 1 0 .6 7
4 .5 6 0 .6 1
1 lath osterol
3 .4 0 2 .7 7 3 .3 0 3 .1 3 2 .4 8 3 .1 6 2 .5 9 2 .6 2 2 .7 2 2.91 0 .3 4 2 .6 3 3 .1 0 2 .7 2 2 .6 2 2 .9 6 2 .4 3 2 .5 4 2.81 2 .2 8 2 .6 8 0 .2 6 3 .6 9 2 .8 0 2 .1 6 3 .4 0 2 .8 0 2 .1 7 3 .0 2 2 .4 2 2 .2 0 2 .7 4 0 .5 6 2 .1 3 2 .1 6 2 .3 6 2 .0 7 1 .9 9 2 .0 5 2 .0 6 2 .0 8 2 .0 5 2 .1 0 0.11 2 .6 0 2 .4 9 2 .3 5 2 .7 4 2 .6 7 2 .4 4 2 .5 3 2 .3 3 2 .1 3 2 .4 8 0 .1 9 2 .4 0 2 .5 4 2 .5 0 2 .2 2 2 .2 8 2.61 2.01 2 .1 3 2 .5 2 2 .3 6 0.21 1 .6 3 1 .7 8 1.71 1 .7 0 1.81 1 .7 4 1 .7 2 1 .8 5 1 .5 5 1 .7 2 0 .0 9
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A105 of A172
group
i i 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 AVERAGE SD
cage#
1,2 3 ,4
5 1,2 3 ,4 5 1,2 3 ,4 5
GC sam ple#
1:1-1 1 :1 -2 1 :1 -3 2:1-1 2 :1 -2 2 :1 -3 3:1-1 3 :1 -2 3 :1 -3
neutral sterols (pm ol/g feces)
ca m p e ste ro l stig m a ste ro l b -sito ste ro l b -sito sta n o l
0 .6 3
0.21
1 .7 0
0 .0 0
0 .7 2
0 .2 5
1 .8 4
0 .0 0
0 .6 0
0 .2 0
1.51
0 .0 0
0 .7 8
0 .1 8
1 .9 3
0 .0 0
0 .7 3
0 .2 5
1 .7 9
0 .0 0
0 .7 5
0 .2 4
1 .9 4
0 .0 0
0 .6 8
0.21
1 .6 4
0 .0 0
0 .6 6
0 .1 6
1 .6 4
0 .0 0
0 .5 8
0 .2 0
1 .3 7
0 .0 0
neutral sterols total (pm ol/g feces)
2 .5 4 2 .8 1 2 .3 1 2 .9 0 2 .7 7 2 .9 3 2 .5 4 2 .4 6 2 .1 5
total feces
(g) 3 .0 7 5 2 .4 8 9 7 2 .2 9 4 6 4 .6 9 2 3 .7 7 8 9 3 .4 1 0 6 3 .5 7 5 4 2 .8 7 8 1 2 .7 5 8 9
average w eight
(g) 1 2 3 .6 8 7 .2 5 8 8 .5 5 1 2 2 .4 5 8 6 .5 5 8 8 .2 1 2 1 .6 5 8 6 .5 5 8 8 .0 5
days
2 2 2 3 3 3 2 2 2
6 7 ,8 9 ,1 0
6 7 ,8 9 ,1 0
6 7 ,8 9 ,1 0
1 :2 -4 1 :2 -5 1 :2 -6 2 :2 -4 2 :2 -5 2 :2 -6 3 :2 -4 3 :2 -5 3 :2 -6
0 .6 9 0 .6 4 0 .7 3 0 .7 0 0 .7 4 0 .8 0 0 .7 0 0 .5 6 0 .7 2
0 .2 5 0 .2 4 0 .2 5 0 .1 2 0 .2 7 0 .1 7 0 .2 3 0 .1 6 0 .2 5
1 .8 0 1 .6 4 1 .9 2 1 .7 6 1 .8 5 2 .0 7 1 .6 9 1 .2 5 1.81
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
2 .7 4 2 .5 2 2 .9 1 2 .5 9 2 .8 6 3 .0 4 2 .6 2 1 .9 7 2 .7 8
2 .7 6 1 9 4 .0 5 8 1 2 .0 9 3 5 4 .0 7 5 6 .3 7 1 3 .4 0 7 4 2 .9 2 7 9 4 .3 9 8 5 2 .4 5 7 5
9 4 .5 5 1 2 6 .6 8 2 .2 9 4 .2 5 1 2 6 .4 5 82.1
94 1 2 6 .6 8 1 .8 5
2 2 2 3 3 3 2 2 2
1 1 ,1 2 ,1 3 15
1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7
1 :3 -7 1 :3 -8 1 :3 -9 2 :3 -7 2 :3 -8 2 :3 -9 3 :3 -7 3 :3 -8 3 :3 -9
0 .7 1 0 .6 7 0 .7 1 0 .7 4 0 .6 6 0 .7 1 0 .7 0 0 .6 4 0 .6 4
0 .2 6 0 .2 4 0 .1 3 0 .2 6 0 .2 0 0 .1 3 0 .2 4 0 .2 0 0 .1 4
1 .8 2 1 .6 9 1 .8 2 1 .8 4 1 .6 3 1 .8 5 1 .6 5 1 .5 2 1 .5 6
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
2 .7 8 2 .6 0 2 .6 5 2 .8 3 2 .4 9 2 .7 0 2 .5 9 2 .3 6 2 .3 3
3 .4 5 9 7 2 .2 9 9 4 2 .3 4 5 4 5 .8 1 4 7 3 .7 7 1 8 3 .7 7 2 7 4 .0 2 5 2 .5 2 6 5 2 .6 9 0 5
118 8 3 .5 5 8 2 .8 1 1 8 .7 5 8 3 .5 8 3 .0 5 1 1 9 .6 8 4 .2 8 3 .4 5
2 2 2 3 3 3 2 2 2
18 19 2 0 ,2 1 18 19 2 0 ,2 1 18 19 2 0 ,2 1
1 :4 -1 0 1 :4 -1 1 1 :4 -1 2 2 :4 -1 0 2 :4 -1 1 2 :4 -1 2 3 :4 -1 0 3 :4 -1 1 3 :4 -1 2
0 .7 0 0 .5 3 0 .6 6 0 .6 7 0 .7 1 0 .7 7 0 .5 4 0 .6 6 0 .6 1
0 .2 6 0 .0 8 0 .0 9 0 .1 0 0 .1 0 0 .0 8 0 .0 7 0 .0 8 0 .0 7
1 .7 8 1 .3 4 1 .6 3 1 .6 8 1 .6 8 1 .8 6 1 .2 5 1 .5 3 1 .4 2
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
2 .7 4 1 .9 4 2 .3 8 2 .4 5 2 .4 9 2 .7 2 1 .8 6 2 .2 7 2 .1 0
1 .6 8 9 3 .1 4 3 2 3 .2 5 4 5 2 .7 1 3 3 4 .6 4 0 7 5 .2 0 7 5 2 .0 4 1 3 .4 4 6 4 3 .9 5 4 9
5 9 .5 1 1 6 .4 1 1 2 .2 5 8 .8 5 1 1 6 .5 5 1 1 1 .8 5 5 8 .7 1 1 7 .4 5 1 1 2 .9
2 2 2 3 3 3 2 2 2
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
1 :5 -1 3 1 :5 -1 4 1 :5 -1 5 2 :5 -1 3 2 :5 -1 4 2 :5 -1 5 3 :5 -1 3 3 :5 -1 4 3 :5 -1 5
0 .7 0 0 .5 9 0 .6 4 0 .7 2 0 .6 1 0 .7 9 0 .5 9 0 .5 1 0 .5 8
0 .1 3 0 .2 4 0.11 0.11 0 .1 0 0 .1 4 0 .2 2 0 .2 0 0.11
1 .8 0 1 .5 0 1 .7 6 1 .7 4 1 .4 9 1 .9 7 1 .4 7 1 .2 5 1 .3 6
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
2 .6 4 2 .3 2 2 .5 1 2 .5 7 2 .1 9 2 .9 0 2 .2 7 1 .9 6 2 .0 4
3 .1 9 5 2 .9 4 0 8 2 .6 3 9 9 4 .9 7 1 2 4 .4 8 3 3 3 .7 6 7 3 .6 1 1 3 3 .3 5 2 2 2 .7 0 3 4
1 1 0 .7 8 8 .3 5 8 4 .6 1 1 1 .6 8 8 .5 8 5 .5 5 1 1 2 .8 5 8 8 .9 8 5 .5
2 2 2 3 3 3 2 2 2
2 8 ,2 9 31
3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2
1 :6 -1 6 1 :6 -1 7 1 :6 -1 8 2 :6 -1 6 2 :6 -1 7 2 :6 -1 8 3 :6 -1 6 3 :6 -1 7 3 :6 -1 8
0 .6 6 0 .6 8 0 .6 6 0 .6 0 0 .7 7 0 .6 2 0 .5 3 0 .5 9 0 .6 6
0 .0 9 0 .0 9 0 .0 9 0 .1 0 0 .0 7 0 .0 9 0 .0 7 0 .0 7 0 .0 8
1.71 1.71 1 .7 3 1 .5 5 1.91 1 .5 9 1 .2 7 1 .3 6 1.61
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
2 .4 7 2 .4 8 2 .4 8 2 .2 5 2 .7 5 2 .2 9 1 .8 7 2 .0 2 2 .3 4
2 .5 0 3 6 2 .7 0 8 3 2 .3 5 5 4 3 .9 6 8 4 3 .9 6 7 8 3 .5 6 6 6 2 .9 0 7 6 3 .2 4 4 9 2 .7 6 4 3
87.1 1 1 2 .8 8 5 .4 8 7 .1 5 1 1 2 .4 5 8 4 .5 5 8 7 .6 5 1 1 1 .9 5 8 4 .6 5
2 2 2 3 3 3 2 2 2
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
1 :7 -1 9 1 :7 -2 0 1 :7 -2 1 2 :7 -1 9 2 :7 -2 0 2 :7 -2 1 3 :7 -1 9 3 :7 -2 0 3 :7 -2 1
0 .7 5 0 .6 8 0 .7 2 0 .6 4 0 .7 8 0 .6 9 0 .5 9 0 .5 9 0 .6 0
0 .1 0 0 .0 9 0 .0 9 0.31 0 .1 0 0 .2 4 0 .0 7 0 .0 7 0 .0 5
1.91 1 .7 2 1 .7 7 1 .4 7 1 .8 4 1 .6 4 1 .3 8 1 .4 2 1 .3 9
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
2 .7 6 2 .4 9 2 .5 7 2 .4 2 2 .7 3 2 .5 7 2 .0 4 2 .0 8 2 .0 5
1 .9 6 9 7 2 .0 7 9 8 2 .6 7 0 5 3 .4 4 7 7 3 .2 6 4 6 4 .3 0 5 9 2 .5 1 1 7 2 .4 3 6 9 3 .4 1 0 7
8 1 .3 5 8 2 .2 5 1 0 1 .0 5 8 2 .3 5 8 2 .7 5 1 0 1 .9 5 8 3 .5 8 3 .3 5 1 0 3 .2
2 2 2 3 3 3 2 2 2
total neutrol sterols excretion (pm ol/100 g r m ouse/day) 3 .1 6 4 .0 1 2 .9 9 3 .7 0 4 .0 4 3 .7 8 3 .7 3 4 .0 9 3 .3 7 3 .6 5 0 .3 9 4 .0 0 4 .0 3 3 .7 0 3 .7 3 4 .8 0 4 .2 1 4 .0 8 3 .4 2 4 .1 8 4 .0 2 0 .3 9 4 .0 7 3 .5 8 3 .7 6 4 .6 2 3 .7 5 4 .0 8 4 .3 6 3 .5 3 3 .7 6 3 .9 5 0 .3 7 3 .8 9 2 .6 2 3 .4 5 3 .7 7 3 .3 1 4 .2 1 3 .2 4 3 .3 3 3 .6 8 3 .5 0 0 .4 6 3 .8 1 3 .8 6 3 .9 2 3 .8 2 3 .7 0 4 .2 5 3 .6 4 3 .6 9 3 .2 3 3 .7 7 0 .2 7 3 .5 4 2 .9 8 3 .4 3 3 .4 2 3 .2 3 3 .2 2 3 .1 0 2 .9 3 3 .8 3 3 .3 0 0 .2 9 3 .3 5 3 .1 4 3 .4 0 3 .3 7 3 .5 9 3 .6 2 3 .0 7 3 .0 5 3 .3 8 3 .3 3 0 .2 1
cam pesterol 2 4 .9 6 2 5 .4 8 2 5 .9 4 2 7 .0 0 2 6 .2 7 2 5 .6 6 2 6 .9 1 2 6 .9 3 2 6 .9 5 2 6 .2 3 0 .7 6 2 5 .1 2 2 5 .5 3 2 5 .1 8 2 7 .1 4 2 5 .7 8 2 6 .2 7 2 6 .6 4 2 8 .3 2 2 6 .0 2 2 6 .2 2 1 .0 3 2 5 .3 8 2 5 .7 6 2 6 .7 1 2 6 .0 4 2 6 .4 3 2 6 .4 8 2 6 .9 4 2 7 .0 7 2 7 .5 1 2 6 .4 8 0 .6 7 2 5 .5 8 2 7 .2 0 2 7 .6 5 2 7 .4 6 2 8 .4 9 2 8 .2 8 2 8 .7 9 2 8 .9 2 2 9 .1 1 2 7 .9 4 1.11 2 6 .5 5 2 5 .2 4 2 5 .4 0 2 7 .7 8 2 7 .7 0 2 7 .1 0 2 5 .8 6 2 5 .8 0 2 8 .2 6 2 6 .6 3 1 .1 2 2 6 .8 9 2 7 .3 5 2 6 .6 6 2 6 .8 5 2 8 .0 5 2 6 .9 5 2 8 .4 7 2 8 .9 8 2 8 .1 3 2 7 .5 9 0 .8 3 2 7 .1 5 2 7 .3 5 2 7 .8 7 2 6 .3 7 2 8 .7 7 2 6 .7 8 2 8 .8 2 2 8 .5 4 2 9 .2 5 2 7 .8 8 1 .0 2
% o f neutral sterols
s tig m a ste ro l b -sito s te ro l
8 .3 3
6 6 .7 1
8 .9 1
6 5 .6 1
8 .7 7
6 5 .2 9
6 .3 2
6 6 .6 8
9 .1 5
6 4 .5 8
8 .1 5
6 6 .1 8
8 .4 5
6 4 .6 3
6 .4 9
6 6 .5 7
9 .2 1
6 3 .8 5
8 .2 0
6 5 .5 7
1 .0 8
1 .0 5
9 .2 9
6 5 .5 9
9 .4 0
6 5 .0 7
8 .7 2
6 6 .1 0
4 .7 7
6 8 .0 9
9 .4 5
6 4 .7 7
5 .6 9
6 8 .0 5
8 .7 0
6 4 .6 6
8 .2 1
6 3 .4 7
8 .8 4
6 5 .1 3
8 .1 2
6 5 .6 6
1 .7 0
1 .5 4
9 .2 0
6 5 .4 2
9 .0 9
6 5 .1 5
4 .9 1
6 8 .3 8
9 .0 7
6 4 .8 9
8 .0 2
6 5 .5 5
4 .9 5
6 8 .5 7
9 .3 9
6 3 .6 7
8 .4 8
6 4 .4 6
5 .8 1
6 6 .6 8
7 .6 6
6 5 .8 6
1 .8 9
1 .6 9
9 .6 6
6 4 .7 6
3 .8 7
6 8 .9 3
3 .6 7
6 8 .6 8
3 .9 6
6 8 .5 8
4 .0 1
6 7 .5 0
3 .0 9
6 8 .6 3
3 .9 1
6 7 .3 0
3 .4 2
6 7 .6 6
3 .4 2
6 7 .4 6
4 .3 3
6 7 .7 2
2 .0 2
1 .2 8
5 .0 9
6 8 .3 7
1 0 .2 3
6 4 .5 3
4 .4 7
7 0 .1 4
4 .4 5
6 7 .7 7
4 .4 1
6 7 .8 8
4 .8 1
6 8 .0 9
9 .4 9
6 4 .6 5
1 0 .3 4
6 3 .8 6
5 .1 9
6 6 .5 5
6 .5 0
6 6 .8 7
2 .6 7
2 .1 2
3 .7 0
6 9 .4 1
3 .7 3
6 8 .9 2
3 .7 0
6 9 .6 4
4 .2 7
6 8 .8 8
2 .5 0
6 9 .4 4
3 .8 4
6 9 .2 1
3 .6 5
6 7 .8 8
3 .6 5
6 7 .3 7
3 .3 8
6 8 .4 9
3 .6 0
6 8 .8 1
0 .4 7
0 .7 6
3 .5 6
6 9 .2 8
3 .4 2
6 9 .2 2
3 .3 6
6 8 .7 7
1 2 .8 4
6 0 .7 9
3 .6 6
6 7 .5 7
9 .3 2
6 3 .9 1
3 .3 4
6 7 .8 4
3 .1 8
6 8 .2 8
2 .6 8
6 8 .0 6
5 .0 4
6 7 .0 8
3 .5 5
2 .8 5
1 b -sito sta n o l
0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0 0 .0 0
Appendix to TNO-report of 3M-02
Final version
04-12-2006
sterols
total neutral sterols coprostanol coprostanon cholesterol cholestanol lathosterol
total phvtosterols campesterol stigmasterol b-sitosterol b-sitostanol
sterols
total neutral sterols coprostanol coprostanon cholesterol cholestanol lathosterol
total phvtosterols campesterol stigmasterol b-sitosterol b-sitostanol
sterols
total neutral sterols coprostanol coprostanon cholesterol cholestanol lathosterol
total phvtosterols campesterol stigmasterol b-sitosterol b-sitostanol
group 1 vs 2 nn p 9 9 0.050 9 9 1.000 9 9 1.000 9 9 0.040 9 9 0.258 9 9 0.190 9 9 0.113 9 9 0.863 9 9 0.666 9 9 0.931 9 9 1.000 group 2 vs 4
nn p 9 9 0.040 9 9 1.000 9 9 1.000 9 9 0.077 9 9 0.489 9 9 <0.001 9 9 0.024 9 9 0.006 9 9 0.004 9 9 0.024 9 9 1.000 group 3 vs 7 nn p 9 9 0.190 9 9 1.000 9 9 1.000 9 9 0.031 9 9 <0.001 9 9 <0.001 9 9 <0.001 9 9 0.008 9 9 0.040 9 9 0.190 9 9 1.000
group 1 vs 3 nn p 9 9 0.077 9 9 1.000 9 9 1.000 9 9 0.863 9 9 0.546 9 9 0.489 9 9 0.222 9 9 0.546 9 9 0.863 9 9 1.000 9 9 1.000 group 2 vs 5 nn p 9 9 0.666 9 9 1.000 9 9 1.000 9 9 0.605 9 9 0.340 9 9 0.113 9 9 0.136 9 9 0.489 9 9 0.387 9 9 0.387 9 9 1.000 group 4 vs 5 nn p 9 9 0.113 9 9 1.000 9 9 1.000 9 9 0.094 9 9 0.605 9 9 <0.001 9 9 0.190 9 9 0.031 9 9 0.002 9 9 0.387 9 9 1.000
Sterol excretion, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5 group 1 vs 6
nn p nn p nn
p
9 9 <0.001 9 9 0.050 9
0.161
9 9 1.000 9 9 1.000 9
1.000
9 9 1.000 9 9 1.000 9
1.000
9 9 <0.001 9 9 0.546 9
0.489
9 9 0.050 9 9 1.000 9
0.002
9 9 <0.001 9 9 0.008 9
0.010
9 9 0.489 9 9 0.730 9
0.077
9 9 0.002 9 9 0.489 9
0.014
9 9 0.004 9 9 0.258 9
<0.001
9 9 0.001 9 9 0.190 9
<0.001
9 9 1.000 9 9 1.000 9
1.000
group 2 vs 6 group 2 vs 7 group 3 vs 4
nn p nn p nn
p
9 9 0.666 9 9 0.113 9 9 0.006
9 9 1.000 9 9 1.000 9 9 1.000
9 9 1.000 9 9 1.000 9 9 1.000
9 9 0.040 9 9 0.003 9 9 0.003
9 9 0.050 9 9 <0.001 9 9 0.136
9 9 0.008 9 9 <0.001 9 9 <0.001
9 9 0.001 9 9 <0.001 9 9 0.063
9 9 0.008 9 9 0.003 9 9 0.006
9 9 <0.001 9 9 0.031 9 9 0.004
9 9 <0.001 9 9 0.113 9 9 0.024
9 9 1.000 9 9 1.000 9 9 1.000
group 4 vs 6 group 4 vs 7 group 5 vs 6
n n E__ n n p n n p
9 9 0.006 9 9 <0.001 9 9 0.190
9 9 1.000 9 9 1.000 9 9 1.000
9 9 1.000 9 9 1.000 9 9 1.000
9 9 <0.001 9 9 <0.001 9 9 0.489
9 9 <0.001 9 9 <0.001 9 9 0.258
9 9 0.011 9 9 <0.001 9 9 0.297
9 9 0.190 9 9 0.340 9 9 0.004
9 9 0.258 9 9 0.796 9 9 0.094
9 9 0.436 9 9 0.297 9 9 <0.001
9 9 0.040 9 9 0.730 9 9 0.024
9 9 1.000 9 9 1.000 9 9 1.000
group 1 vs 7 nn p 9 9 0.605 9 9 1.000 9 9 1.000 9 9 0.024 9 9 <0.001 9 9 <0.001 9 9 0.063 9 9 0.003 9 9 0.050 9 9 0.040 9 9 1.000 group 3 vs 5 nn p 9 9 0.161 9 9 1.000 9 9 1.000 9 9 0.730 9 9 0.931 9 9 0.387 9 9 0.546 9 9 0.730 9 9 0.436 9 9 0.546 9 9 1.000 group 5 vs 7 nn p 9 9 0.094 9 9 1.000 9 9 1.000 9 9 0.190 9 9 0.063 9 9 <0.001 9 9 0.001 9 9 0.031 9 9 0.024 9 9 0.605 9 9 1.000
group 2 vs 3 nn p 9 9 0.489 9 9 1.000 9 9 1.000 9 9 0.222 9 9 0.190 9 9 0.931 9 9 0.796 9 9 0.387 9 9 0.605 9 9 0.730 9 9 1.000 group 3 vs 6 nn p 9 9 0.863 9 9 1.000 9 9 1.000 9 9 0.340 9 9 0.008 9 9 0.190 9 9 0.001 9 9 0.014 9 9 <0.001 9 9 0.001 9 9 1.000 group 6 vs 7 nn p 9 9 0.297 9 9 1.000 9 9 1.000 9 9 0.113 9 9 0.004 9 9 <0.001 9 9 0.863 9 9 0.546 9 9 0.387 9 9 0.094 9 9 1.000
p<0.05
Page A106 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
E4. Fecal fatty acid measurements
1 :1-1 w e ig h t feces
8.6
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
13257 256605
2203 231285 22933
1279 2731
%
2.5 4 8 .4 0.4 4 3 .6 4.3 0.2 0.5
Mg fa tty acid
13.9 269 .5
2.3 242 .9 24.1
1.3 2.9
ng fa tty acid m g feces 1619 31340 269 28247 2801 156 334
nm ol fa tty acid/ m g feces 7 .0 9 1 2 2 .2 3 1.06 9 9 .2 9 9.91 0 .5 6 1.20
to ta l
530293
100 1 5 5 7 0 1 64766
2 4 1 .3 3
C 15:0
1 76166 1
I 1:1-2
w e ig h t fe ce s
10.1
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
16324 404265
3337 411875 37807
2492 1091
%
1.9 46.1 0.4 4 7 .0 4.3 0.3 0.1
Mg fa tty acid
14.8 366 .5
3.0 373 .4 3 4 .3
2.3 1.0
ng fa tty acid m g feces 1465 36288 300 36971 3394 224 98
nm ol fa tty acid/ m g feces 6.42 1 4 1 .5 3 1.18 1 2 9 .9 5 12.01 0 .8 0 0 .3 5
to ta l
877191
100 1 7 9 5 3 1 78739
2 9 2 .2 4
C 15:0
1 88241 1
1:1-3
w e ig h t feces
8.8
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
18959 482473
4337 520971 55946
3057 6151
%
1.7 44.2 0.4 47.7 5.1 0.3 0.6
Mg fa tty acid
14.2 362 .4
3.3 391 .3 4 2 .0
2.3 4.6
ng fa tty acid m g feces 1618 41183 370 44469 4775 261 525
nm ol fa tty acid/ m g feces 7 .0 9 1 6 0 .6 2 1.46 1 5 6 .3 0 16.90 0 .9 3 1.89
to ta l
1091894
100 1 820 .2 1 93201
3 4 5 .1 8
C 15:0
1 106504 1
2:1 -1 w e ig h t fe ce s
7.6
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
8193 210520
1596 214647 24494
1701 2635
%
1.8 4 5 .4 0.3 4 6 .3 5.3 0.4 0.6
Mg f a tty a c id
7.5 1 9 3 .9
1.5 1 9 7 .7 2 2 .6
1.6 2.4
ng fa tty acid m g feces 993 25508 193 26008 2968 206 319
nm ol fa tty acid/ m g feces 4 .3 5 99.49 0 .7 6 91.42 10.51 0 .7 3 1.15
to ta l
1 463786 T 100
1 4274
1 56196 r
208 .40
C 15:0
1 86874 1
2:1-2
w e ig h t fe c e s
5 .7
mg
|
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
7613 201403
1437 196561 19865
1352 2147
%
1.8 4 6 .8 0.3 4 5 .7 4.6 0.3 0.5
Mg f a tty a c id 6.7 1 7 6 .8 1.3 1 7 2 .5 1 7 .4 1.2 1.9
ng fa tty acid m g feces 1172 31014 221 30268 3059 208 331
nm ol fa tty acid/ m g feces 5 .1 3 1 2 0 .9 6 0 .8 7 1 0 6 .3 9 1 0 .8 3 0 .7 4 1.19
to ta l
1 430378 T 100
1 3778 1 66274 T
2 4 6 .1 1
C 15:0
1 91143 1
2:1-3
w e ig h t fe ce s
7.2
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
9801 284195
2725 348778 35118
2252 4534
%
1.4 4 1 .3 0.4 5 0 .7 5.1 0.3 0.7
Mg f a tty a c id 9.1 2 6 3 .4 2.5 3 2 3 .3 3 2 .6 2.1 4.2
ng fa tty acid m g feces 1262 36587 351 44901 4521 290 584
nm ol fa tty acid/ m g feces 5 .5 2 1 4 2 .7 0 1 .3 8 1 5 7 .8 3 1 6 .0 0 1 .0 3 2 .1 0
to ta l
1 687403 T 100
1 6 37 2 1 88496 T
326 .56
C 15:0
1 86307 1
m olw
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
228 .4 256 .4 254 .4 284 .5 282 .5 280 .5 2 7 8 .5
M Y S T R IC A C ID P A LM ITIC A C ID P A LM IT O LE IC A C ID S T E A R IC A C ID O LE IC A C ID LIN O LE IC A C ID LIN O LE N IC A C ID
Page A107 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
| 3:1-1
w eight feces
7.3
mg
C 1 4:0 C 1 6:0 C 1 6:1 C 1 8:0 C 1 8:1 C 1 8 :2 C 1 8 :3
area
5463 156085
1332 248855 26880
1566 4158
%
1.2 35.1 0.3 56.0 6.0 0.4 0.9
Mg fa tty acid
7.5 2 1 3 .4
1.8 3 4 0 .2 36.8
2.1 5.7
ng fatty acid m g feces 1023 29233 249 46607 5034 293 779
nm ol fatty acid/ m g feces 4.48 114.01 0.98 163.82 17.82 1.05 2.80
to ta l
| 444339 |
100
| 607.5 | 83219 |
3 0 4 .9 6
|
C 15:0
I 58514 1
I 3:1-2
w eight feces
5.7
;
mg
C 1 4:0 C 1 6:0 C 1 6:1 C 1 8:0 C 1 8:1 C 1 8 :2 C 1 8 :3
area
7866 223765
1702 248550 26968
1646 2844
%
1.5 43.6 0.3 48.4 5.3 0.3 0.6
Mg fa tty acid 7.3 2 0 8 .6 1.6 2 3 1 .7 25.1 1.5 2.7
ng fatty acid m g feces 1287 36598 278 40651 4411 269 465
nm ol fatty acid/ m g feces 5.63 142.74 1.09 142.89 15.61 0.96 1.67
to ta l
| 513341 | 100
| 478.6 | 83959 |
3 1 0 .5 9
|
C 15:0
I 85813 1
3 j 3 ___________ __________________________________________________
w eight feces
8.3
mg
C 1 4:0 C 1 6:0 C 1 6:1 C 1 8:0 C 1 8:1 C 1 8 :2 C 1 8 :3
area
11108 319405
2509 380522 37736
2443 5057
%
1.5 42.1 0.3 50.1 5.0 0.3 0.7
Mg fa tty acid
9.5 2 7 4 .4
2.2 3 2 6 .9 32.4
2.1 4.3
ng fatty acid m g feces 1150 33063 260 39390 3906 253 523
nm ol fatty acid/ m g feces 5.03 128.95 1.02 138.45 13.83 0.90 1.88
to ta l
I 758780 |
100
| 651.9 | 78546 |
2 9 0 .0 7
|
C 15:0
I 93112 1
1:2-4
w eight feces
13.4
mg
C 1 4 :0 C 1 6 :0 C16:1 C 1 8 :0 C18:1 C 1 8 :2 C 1 8 :3
area
27893 881387
8053 1181866 109775
5394 17094
%
1.2 39.5 0.4 53.0 4.9 0.2 0.8
Mg fa tty acid
20.7 654.1
6.0 877.0 81.5
4.0 12.7
ng fatty acid mg feces
1545 48810
446 65450 6079
299 947
nm ol fatty acid/ mg feces
6.76 190.37
1.75 2 3 0 .0 5 21.52
1.06 3.40
to ta l
I 2231462 I
100 | 1655.9 | 123575 |
4 5 4 .9 2
|
C 15:0
I 107806 1
1:2-5
w eight feces
9.2
mg
C 1 4 :0 C 1 6 :0 C16:1 C 1 8 :0 C18:1 C 1 8 :2 C 1 8 :3
area
31737 923618
93 1135022 147397
7383 16098
%
1.4 40.8 0.0 50.2 6.5 0.3 0.7
Mg fa tty acid 19.1 557.2 0.1 684.7 88.9 4.5 9.7
ng fatty acid mg feces 2081 60560 6 74421 9665 484 1056
nm ol fatty acid/ mg feces 9.11 2 3 6 .1 9 0.02 2 6 1 .5 9 34.21 1.73 3.79
to ta l
I 2261348 I
100 | 1364.1 | 148272 |
5 4 6 .6 4
|
C 15:0
I 132620 1
1 :2-6 w eight feces
9
area
C 1 4 :0 C 1 6 :0 C16:1 C 1 8 :0 C18:1 C 1 8 :2 C 1 8 :3
10087 262021
2302 272616 27573
1825 3059
to ta l
I 579483 I
C 15:0
I 123199 1
mg
%
1.7 45.2 0.4 47.0 4.8 0.3 0.5
Mg fa tty acid
6.6 170.1
1.5 177.0 17.9
1.2 2.0
ng fatty acid mg feces
728 18905
166 19669 1989
132 221
nm ol fatty acid/ mg feces
3.19 73.73 0.65 69.14 7.04 0.47 0.79
100 | 376.3 | 41810 |
1 5 5 .0 1
Page A108 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:2-4
w eight feces
9.7
mg
C 1 4:0 C 1 6:0 C 1 6:1 C 1 8:0 C 1 8:1 C 1 8:2 C 1 8:3
area
18615 488224
4357 581814 60179
3098 7073
%
1.6 42.0 0.4 50.0 5.2 0.3 0.6
Mg fa tty acid 15.3 4 0 1 .8 3.6 4 7 8 .8 49.5 2.5 5.8
ng fatty acid mg feces 1579 41421 370 49361 5106 263 600
nm ol fatty acid/ mg feces 6.91 161.55 1.45 173.50 18.07 0.94 2.15
to ta l
I 1163360 I
100 | 957.4 | 98699 |
3 6 4 .5 8
|
C 15:0
I 97212 I
3:2-4
w e ig h t fe ce s
8.6
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
18311 420511
3649 545499 56501
3001 7025
%
1.7 3 9 .9 0.3 51.7 5.4 0.3 0.7
Mg fa tty acid 15.1 3 4 6 .4 3.0 4 4 9 .4 46.5 2.5 5.8
ng fa tty acid m g feces 1754 40284 350 52257 5413 287 673
nm ol fa tty acid/ m g feces 7.68 157.11 1.37 1 8 3 .6 8 19.16 1.02 2.42
to ta l
| 1054497 | 100 | 868.7 | 101017 |
372~45
C 15:0
| 97105 |
w eight feces
C 1 4:0 C 1 6:0 C 1 6:1 C 1 8:0 C 1 8:1 C 1 8:2 C 1 8:3
9.3
area
18128 549910
5378 733331 85796
4289 10396
mg
%
1.3 39.1 0.4 52.1 6.1 0.3 0.7
Mg fa tty acid 14.7 4 4 4 .5 4.3 5 9 2 .7 69.3 3.5 8.4
ng fatty acid mg feces 1575 47793 467 63734 7457 373 904
nm ol fatty acid/ mg feces 6.90 186.40 1.84 2 2 4 .0 2 26.39 1.33 3.24
to ta l
J1 1 4 0 7 2 2 8 11 100 11 1 1 3 7 .4 11 1 2 2 3 0 2 1
4 5 0 .1 2
1
C 15:0
11 9 8 9 7 8 1
2:2-6
w eight feces
C 1 4:0 C 1 6:0 C 1 6:1 C 1 8:0 C 1 8:1 C 1 8:2 C 18:3
59
area
5981 103637
790 97059 10984
884 1019
mg
%
2.7 47.0 0.4 44.0 5.0 0.4 0.5
i
Mg fa tty acid 4.9 84.2 0.6 78.9 8.9 0.7 0.8
ng fatty acid mg feces 824 14272 109 13366 1513 122 140
nm ol fatty acid/ mg feces 3.61 55.66 0.43 46.98 5.35 0.43 0.50
total
220354
100 1 T T ^ 1 30345
112.97
C15:0
1 98463 1
w e ig h t fe c e s
7.7
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
14837 453886
4567 591094 63874
3296 7991
%
1.3 3 9 .8 0.4 5 1 .9 5.6 0.3 0.7
Mg fa tty acid 11.7 3 5 6 .9 3.6 4 6 4 .8 50.2 2.6 6.3
ng fa tty acid m g feces 1515 46352 466 60364 6523 337 816
nm ol fa tty acid/ m g feces 6.63 1 8 0 .7 8 1.83 2 1 2 .1 8 2 3 .0 9 1.20 2.93
to ta l
J1 1 1 3 9 5 4 5 11 1 0 0
11 8 9 6 .1 11 1 1 6 3 7 4 11
4 2 8 .6 5
1
C 15:0
1 101736 1
3:2-6
w e ig h t fe c e s
8 .4
mg
area
%
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
5585 169177
1364 196952 20806
1530 2302
1.4 4 2 .5 0.3 4 9 .5 5.2 0.4 0.6
to ta l
397716
100
C 15:0
1 9098T I
Mg fa tty acid 4.9 1 4 8 .8 1.2 1 7 3 .2 18.3 1.3 2.0
ng fa tty acid m g feces 585 17709 143 20617 2178 160 241
nm ol fa tty acid/ m g feces 2.56 6 9 .0 7 0.56 7 2 .4 7 7.71 0.57 0.87
349 7 I 41633
1 5 3 .8 0
Page A109 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:3-7
w e ig h t feces
11
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
14517 354931
3055 369554 36484
2417 4404
%
1.8 4 5 .2 0 .4 47.1 4.6 0.3 0.6
Mg f a tty acid
10.1 245 .8
2.1 256 .0 2 5 .3
1.7 3.1
ng fa tty acid m g feces 914 22349 192 23269 2297 152 277
nm ol fa tty acid/ m g feces 4 .0 0 8 7 .1 6 0 .7 6 8 1 .7 9 8 .1 3 0 .5 4 1.00
to ta l
I 785362 I
100 | 544 .0 | 49451 |
183 .38
|
C 1 5:0
I 115502 1
237
w e ig h t fe ce s
8.3
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
7895 202071
1616 212839 21090
1476 2397
%
1.8 4 5 .0 0.4 4 7 .4 4.7 0.3 0.5
Mg f a tty a c id
6.9 1 7 7 .0
1.4 186 .4 18.5
1.3 2.1
ng fa tty acid m g feces 833 21327 171 22464 2226 156 253
nm ol fa tty acid/ m g feces 3 .6 5 8 3 .1 8 0 .6 7 7 8 .9 6 7 .8 8 0 .5 6 0.91
to ta l
I 449384 I
100
| 393 .7 | 47429 |
175 .80
|
C 1 5:0
I 91324 1
I 1:3-8
w e ig h t fe ce s
11.1
mg
I 2:3-8
w e ig h t fe c e s
7.6
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
20655 613614
5769 774403 72889
4402 10243
%
1.4 4 0 .9 0 .4 5 1 .6 4.9 0.3 0.7
Mg f a tty acid 11.8 350 .2 3.3 441 .9 4 1 .6 2.5 5.8
ng fa tty acid m g feces 1062 31548 297 39815 3747 226 527
nm ol fa tty acid/ m g feces 4 .6 5 123 .04 1.17 1 3 9 .9 5 1 3 .2 7 0.81 1.89
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
13707 374445
3351 433392 46483
2770 5202
%
1.6 4 2 .6 0.4 4 9 .3 5.3 0.3 0.6
Mg f a tty a c id
9.9 269 .9
2.4 312 .4 3 3 .5
2.0 3.8
ng fa tty acid m g feces 1300 35519 318 41111 4409 263 493
nm ol fa tty acid/ m g feces 5 .6 9 1 3 8 .5 3 1 .2 5 1 4 4 .5 0 15.61 0 .9 4 1 .7 7
to ta l
I 1501975 I
100 | 857 .2 | 77221 |
2 8 4 .7 7
|
to ta l
I 879350 I
100
| 633 .9 | 834 14 |
3 0 8 .2 9
|
C 1 5:0
I 140182 1
C 1 5:0
I 110969 1
I 1:3-9
w e ig h t fe ce s
1 3 .4
mg
area
%
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
26223 851207
7949 1186581 108707
7434 16281
1.2 3 8 .6 0 .4 5 3 .8 4.9 0.3 0.7
to ta l
2204382
100
C 15:0
1 144789 I
Mg f a tty acid 14.5 470 .3 4 .4 655 .6 60.1 4.1 9.0
ng fa tty acid m g feces 1081 35098 328 48927 4482 307 671
nm ol fa tty acid/ m g feces 4 .7 3 1 3 6 .8 9 1.29 1 7 1 .9 7 1 5 .8 7 1.09 2.41
121 8.0 1 908 94
334 .26
I 2:3-9
w e ig h t fe c e s
7 .4
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
9500 267057
2386 302932 29071
2020 3014
%
1.5 4 3 .4 0.4 4 9 .2 4.7 0.3 0.5
Mg f a tty a c id
7.5 210 .9
1.9 239 .2 2 3 .0
1.6 2 .4
ng fa tty acid m g feces 1014 28499 255 32327 3102 216 322
nm ol fa tty acid/ m g feces 4 .4 4 1 1 1 .1 5 1 .0 0 1 1 3 .6 3 1 0 .9 8 0 .7 7 1 .1 5
to ta l
1 615980 T 100
1 4 8 6 1 11 6 5 7 3 4 T
243 .12
C 15:0
I 101306 I
Page A110 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
I 3:3-7
w e ig h t fe ce s
8.5
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
7543 193432
1421 205498 21652
1375 2581
%
1.7 4 4 .6 0.3 4 7 .4 5.0 0.3 0.6
Mg fa tty a c id 6 .4 1 6 4 .2 1.2 174 .4 1 8 .4 1.2 2.2
ng fa tty acid m g feces 753 19312 142 20517 2162 137 258
nm ol fa tty acid/ m g feces 3 .3 0 75.32 0 .5 6 72.12 7 .6 5 0 .4 9 0 .9 3
to ta l
I 433502 I 100
| 367 .9 | 43281 |
160 .36
|
C 1 5:0
I 94269 1
I 3:3-8
w e ig h t fe ce s
6.5
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
6822 183086
1710 197892 21000
1164 2282
%
1.6 4 4 .2 0.4 4 7 .8 5.1 0.3 0.6
Mg fa tty a c id
6 .4 1 7 2 .2
1.6 186.1 19.7
1.1 2.1
ng fa tty acid m g feces 987 26486 247 28628 3038 168 330
nm ol fa tty acid/ m g feces 4 .3 2 1 0 3 .3 0 0 .9 7 1 0 0 .6 3 1 0 .7 5 0 .6 0 1.19
to ta l
1 413956 T 100
1 3 80 1 1 59885 r
221 .76
I
C 15:0
I 85077 I
I 3:3-9
w e ig h t fe ce s
14
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
13974 420919
4038 511096 49923
3410 6144
%
1.4 4 1 .7 0.4 5 0 .6 4.9 0.3 0.6
Mg fa tty a c id
1 2 .4 372 .5
3.6 452 .3 4 4 .2
3.0 5.4
ng fa tty acid m g feces 883 26609 255 32310 3156 216 388
nm ol fa tty acid/ m g feces 3 .8 7 1 0 3 .7 8 1 .0 0 1 1 3 .5 7 1 1 .1 7 0 .7 7 1.39
to ta l
1 1009504 I
100
1 8935
1 63818 r
235 .56
1
C 15:0
I 90391 1
1:4-10
w e ig h t feces
8.2
area
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
16048 495415
3727 599026 56734
3322 7505
to ta l
I 1181777 I
C 15:0
I 115946 1
mg
%
1.4 41.9 0.3 50.7 4.8 0.3 0.6
MS fa tty acid 11.1 3 4 1 .8 2.6 4 1 3 .3 39.1 2.3 5.2
ng fa tty acid mg feces 1350 41686 314 50404 4774 280 631
nm ol fa tty acid/ mg feces 5.91 1 6 2 .5 8 1.23 1 7 7 .1 7 16.90 1.00 2.27
100 | 815.4 | 99439 | 367 .06 |
1:4-11
w e ig h t feces
8.8
mg
area
%
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
13693 392497
3202 454998 43482
2667 5468
1.5 42.8 0.3 49.7 4.7 0.3 0.6
to ta l
916007
100
C 15:0
1 128901 I
M3 fa tty acid 8.5 2 4 3 .6 2.0 2 8 2 .4 27.0 1.7 3.4
ng fa tty acid mg feces 966 27681 226 32089 3067 188 386
nm ol fa tty acid/ mg feces 4 .2 3 1 0 7 .9 6 0 .8 9 1 1 2 .7 9 10.86 0.67 1.38
568 5 I 64603
2 3 8 .7 8
1:4-12
w e ig h t feces
9.8
mg
area
%
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
11857 361984
2570 460388 51833
3265 5863
1.3 40.3 0.3 51.3 5.8 0.4 0.7
to ta l
897760
100
C 15:0
1 76774 1
Mg fa tty acid
12.4 3 7 7 .2
2.7 4 7 9 .7 54.0
3.4 6.1
ng fa tty acid mg feces
1261 38489
273 48952 5511
347 623
nm ol fa tty acid/ mg feces
5.52 150.11
1.07 1 7 2 .0 6 19.51
1.24 2 .2 4
9 3 5 5 1 95457
351 .76
1
Page A111 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:4-10
w e ig h t fe ce s
7.1
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
10522 344618
2591 438053 40213
2602 5292
%
1.2 4 0 .8 0.3 5 1 .9 4.8 0.3 0.6
Mg f a tty acid 8.5 2 7 8 .0 2.1 3 5 3 .4 3 2 .4 2.1 4.3
ng fa tty acid mg feces 1196 39156 294 49772 4569 296 601
nm ol fa tty acid/ m g feces 5 .2 3 152.71 1.16 1 7 4 .9 4 16.17 1.05 2 .1 6
to ta l
843891
100 1 680 .8 1 95883
3 5 3 .4 4
C 15:0
1 99169 1
2:4-11
w e ig h t fe ce s
8.7
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
13021 381006
3180 444595 44296
2954 4928
%
1.5 4 2 .6 0.4 49.7 5.0 0.3 0.6
Mg f a tty acid 10.2 2 9 7 .9 2.5 3 4 7 .6 3 4 .6 2.3 3.9
ng fa tty acid mg feces 1170 34238 286 39952 3981 265 443
nm ol fa tty acid/ m g feces 5.12 1 3 3 .5 3 1.12 1 4 0 .4 3 14.09 0 .9 5 1.59
to ta l
893980
100 1 698 .9 1 80335
2 9 6 .8 4
C 15:0
1 102328 1
2:4-12
w e ig h t fe ce s
7.2
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
12246 332981
2528 357246 40701
2826 3646
%
1.6 4 4 .3 0.3 4 7 .5 5.4 0.4 0.5
Mg f a tty acid 9.2 2 4 9 .3 1.9 2 6 7 .5 3 0 .5 2.1 2.7
ng fa tty acid mg feces 1273 34627 263 37150 4232 294 379
nm ol fa tty acid/ m g feces 5 .5 8 1 3 5 .0 5 1.03 1 3 0 .5 8 14.98 1.05 1.36
to ta l
752174
100 1 5 6 3 2 1 78218
2 8 9 .6 3
C 15:0
1 106848 1
3:4-10
w e ig h t fe ces
8.5
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
10172 310431
2449 394129 34766
2260 4580
%
1.3 4 0 .9 0.3 5 1 .9 4.6 0.3 0.6
Mg fa tty acid 9.5 2 8 8 .5 2.3 3 6 6 .3 3 2 .3 2.1 4.3
ng fa tty acid mg feces 1112 33946 268 43098 3802 247 501
nm ol fa tty acid/ mg feces 4.87 1 3 2 .3 9 1.05 1 5 1 .4 9 13.46 0.88 1.80
to ta l
1 758787 T 100
1 7 05 3 1 82973 r
3 0 5 .9 4
1
C 15:0
1 86070 1
3:4-11
w e ig h t fe ces
9.8
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
20169 422311
3653 565589 52273
2913 7488
%
1.9 3 9 .3 0.3 5 2 .6 4.9 0.3 0.7
Mg fa tty acid
17.5 367.1
3.2 4 9 1 .7 4 5 .4
2.5 6.5
ng fa tty acid mg feces
1789 37462
324 50171 4637
258 664
nm ol fa tty acid/ mg feces
7.83 146.11
1.27 1 7 6 .3 5 16.41
0.92 2.39
to ta l
1 1074396 1 100 1 9 3 4 0 1 95305 T 351.28
I
C 15:0
1 92026 1
3:4-12
w e ig h t fe ces
10.7
mg
area
%
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
15056 501243
4206 708410 68397
4333 9322
1.1 38.2 0.3 5 4 .0 5.2 0.3 0.7
to ta l
1310967
100
C 15:0
1 87759
1
Mg fa tty acid 13.7 4 5 6 .9 3.8 6 4 5 .8 6 2 .3 3.9 8.5
ng fa tty acid mg feces 1283 42703 358 60353 5827 369 794
nm ol fa tty acid/ mg feces 5.62 1 6 6 .5 5 1.41 2 1 2 .1 4 2 0 .6 3 1.32 2.85
1195.1 1 111688
4 1 0 .5 1
1
Page A112 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:5-13 w e ig h t feces
8.3
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
13526 393457
3030 473949 44962
3228 5563
%
1.4 4 2 .0 0.3 5 0 .5 4.8 0.3 0.6
Mg fa tty acid 9.5 2 7 5 .2 2.1 3 3 1 .6 3 1 .5 2.3 3.9
ng fa tty acid mg feces 1140 33162 255 39947 3790 272 469
nm ol fa tty acid/ m g feces 4 .9 9 1 2 9 .3 4 1.00 140.41 13.41 0.97 1.68
to ta l
I 937715 I
100
| 656.0 | 790 35 |
2 9 1 .8 1
|
C 1 5:0
I 114357 1
2:5-13 w e ig h t feces
8.1
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
13428 386269
3253 437237 45693
2448 5034
%
1.5 43.2 0.4 4 8 .9 5.1 0.3 0.6
Mg fa tty acid
10.8 3 1 0 .0
2.6 3 5 0 .9 36.7
2.0 4.0
ng fa tty acid mg feces 1331 38276 322 43327 4528 243 499
nm ol fa tty acid/ mg feces 5 .8 3 1 4 9 .2 8 1.27 1 5 2 .2 9 16.03 0.86 1.79
to ta l
I 8 9 3 3 6 2 I 100 | 717.1 | 8 8 5 2 5 |
327 .35
|
C 1 5:0 I 996 70 1
I 1:5-14 w e ig h t fe ce s
11
mg
I
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
33483 1006010
9704 1300166 160875
7669 18973
%
1.3 39.7 0.4 5 1 .3 6.3 0.3 0.7
Mg fa tty acid 19.7 5 9 2 .1 5.7 7 6 5 .3 94.7 4.5 11.2
ng fa tty acid mg feces 1792 53832 519 69572 8608 410 1015
nm ol fa tty acid/ m g feces 7 .8 4 2 0 9 .9 5 2 .0 4 2 4 4 .5 4 3 0 .4 7 1.46 3 .6 5
to ta l
I 2536880 I
100 | 1493.2 | 135749 |
499 .96
|
C 1 5:0
I 135913 1
I 2:5-14 w e ig h t fe ce s
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
9 .9
area
23143 686470
6696 830835 105595
4899 10267
mg
%
1.4 41.2 0.4 4 9 .8 6.3 0.3 0.6
Mg fa tty acid 17.1 5 0 8 .5 5.0 6 1 5 .4 78.2 3.6 7.6
ng fa tty acid mg feces 1731 51359 501 62160 7900 367 768
nm ol fa tty acid/ mg feces 7 .5 8 2 0 0 .3 1 1.97 218 .49 2 7 .9 7 1.31 2 .7 6
to ta l
I 1667905 I
100 | 1235.4 | 124787 |
460 .38
|
C 1 5:0
I 108008 1
1:5-15 w e ig h t fe ce s
8 .3
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
11073 294462
2464 315545 28429
1608 3367
%
1.7 4 4 .8 0.4 4 8 .0 4.3 0.2 0.5
Mg fa tty acid 7.3 195.1 1.6 2 0 9 .0 18.8 1.1 2.2
ng fa tty acid mg feces 884 23500 197 25183 2269 128 269
nm ol fa tty acid/ m g feces 3.87 9 1 .6 5 0.77 8 8 .5 2 8 .0 3 0 .4 6 0 .9 6
to ta l
1 656948 T
100
1 4352
1 52429 T
19427
1
C 15:0
1 120773 I
2 : 5 - 1 5 _________________________________________________________________________________
w e ig h t fe ce s
8 .3
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
7030 128252
1016 106592 11903
760 1127
%
2.7 5 0 .0 0.4 4 1 .5 4.6 0.3 0.4
Mg fa tty acid 6.9 126.1 1.0 1 0 4 .8 11.7 0.7 1.1
ng fa tty acid mg feces 833 15190 120 12624 1410 90 133
nm ol fa tty acid/ mg feces 3 .6 5 59.24 0.47 4 4 .3 7 4 .9 9 0.32 0 .4 8
to ta l
256680
100
252-3 1 30401
1 1 3 .5 3
C 15:0
1 8138 1
Page A113 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
3:5-13
w e ig h t feces
6.8
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
26211 674622
5831 907134 95082
4891 12523
%
1.5 39.1 0.3 5 2 .5 5.5 0.3 0.7
Mg f a tty a c id
10.5 269 .2
2.3 362 .0 3 7 .9
2.0 5.0
ng fa tty acid m g feces 1538 39589 342 53233 5580 287 735
nm ol fa tty acid/ m g feces 6 .7 3 1 5 4 .4 0 1.35 187.11 1 9 .7 5 1.02 2 .6 4
to ta l
I 1726294 I
100 | 688 .9 | 101 3 0 4 |
3 7 3 .0 1
|
C 1 5:0
I 200479 1
I 3:5-14
w e ig h t fe ce s
5 .3
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
12309 339628
3302 368609 45053
2365 4243
%
1.6 4 3 .8 0 .4 4 7 .5 5.8 0.3 0.5
Mg f a tty a c id
10.3 283 .7
2.8 307 .9 3 7 .6
2.0 3.5
ng fa tty acid m g feces 1940 53533 520 58101 7101 373 669
nm ol fa tty acid/ m g feces 8 .4 9 2 0 8 .7 9 2 .0 5 2 0 4 .2 2 2 5 .1 4 1.33 2 .4 0
to ta l
1 775509 T
100
11 6 4 7 9
1 122237 1
452 .42
1
C 15:0
1 95763 1
I 3:5-15
w e ig h t fe ce s
8.1
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
4597 119586
872 128120 13678
939 1419
%
1.7 4 4 .4 0.3 4 7 .6 5.1 0.3 0.5
Mg f a tty a c id 4.7 123.1 0.9 1 3 1 .9 14.1 1.0 1.5
ng fa tty acid m g feces 584 15199 111 16283 1738 119 180
nm ol fa tty acid/ m g feces 2 .5 6 5 9 .2 8 0 .4 4 5 7 .2 3 6 .1 5 0 .4 3 0 .6 5
to ta l
1 269211 T
100
1 2771
1 34215 r
1 2 6 .7 3
I
C 15:0
I 7771 1
1:6-16
w e ig h t fe ce s
11.7
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
20396 664144
5241 854148 80539
5034 11097
%
1.2 40.5 0.3 52.1 4.9 0.3 0.7
MS fa tty acid 12.5 4 0 7 .7 3.2 5 2 4 .4 4 9 .4 3.1 6.8
ng fa tty acid mg feces 1070 34849 275 44819 4226 264 582
nm ol fa tty acid/ mg feces 4 .6 9 1 3 5 .9 2 1.08 1 5 7 .5 4 14.96 0 .9 4 2 .0 9
to ta l C 15:0
I 1640599 |
: :130309
100
I 1 0 0 7 .2 ~ r 86086
3 1 7 .2 1
1:6-17
w e ig h t fe c e s
8 .9
mg
area
%
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
12220 366294
3077 427401 40324
2415 5166
1.4 42.7 0.4 49.9 4.7 0.3 0.6
to ta l
856897
100
C 15:0
1 117016 I
M3 fa tty acid 8.4 2 5 0 .4 2.1 2 9 2 .2 2 7 .6 1.7 3.5
ng fa tty acid mg feces 939 28137 236 32831 3098 186 397
nm ol fa tty acid/ mg feces 4.11 1 0 9 .7 4 0 .9 3 1 1 5 .4 0 10.96 0.66 1.42
585"8 1 65824
2 4 3 .2 3
1:6-18
w e ig h t fe c e s
10.8
mg
area
%
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
17671 535579
4357 659139 67882
4052 8388
1.4 41.3 0.3 50.8 5.2 0.3 0.6
to ta l
1297068
100
C 15:0
1 136015 I
Mg fa tty acid 10.4 3 1 5 .0 2.6 3 8 7 .7 3 9 .9 2.4 4.9
ng fa tty acid mg feces 962 29168 237 35897 3697 221 457
nm ol fa tty acid/ mg feces 4.21 1 1 3 .7 6 0 .9 3 1 2 6 .1 8 13.09 0 .7 9 1.64
7 62 9 1 70639
2 6 0 .5 9
Page A114 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:6-16
weightfeces
12.1
mg
area % Mgfatty ngfattyacid nmolfattyacid/
acid mgfeces mgfeces
C14:0
16368 1.4
12.4 1028
4.50
C16:0 492848 43.0 374.7 30966 120.77
C16:1 3736 0.3 2.8 235 0.92
C18:0 567463 49.5 431.4 35654 125.32
C18:1 55948 4.9 42.5 3515 12.44
C18:2 3689 0.3 2.8 232 0.83
C18:3 6084 0.5 4.6 382 1.37
total 1146136 100 1 871.4 I 72013 266.16
C15:0 1 105228 I
2:6-17
weightfeces
8.5
mg
area % Mgfatty ngfattyacid nmolfattyacid/
acid mgfeces mgfeces
C14:0 7619 2.2 7.2 846 3.70
C16:0 165470 48.1 156.1 18369 71.64
C16:1 1010 0.3 1.0 112 0.44
C18:0 151890 44.1 143.3 16862 59.27
C18:1
15484 4.5
14.6 1719
6.08
C18:2 1040 0.3 1.0 115 0.41
C18:3 1620 0.5 1.5 180 0.65
total 344133 100 1 3247 1 38204 142.20
C15:0 1 84780 1
2:6-18
weightfeces
10.2
mg
area % Mgfatty ngfattyacid nmolfattyacid/ acid mgfeces mgfeces
C14:0 13778 1.8 9.5 928 4.06 C16:0 361552 46.5 248.5 24363 95.02 C16:1 2752 0.4 1.9 185 0.73 C18:0 355381 45.7 244.3 23948 84.17 C18:1 38817 5.0 26.7 2616 9.26 C18:2 2125 0.3 1.5 143 0.51 C18:3 3865 0.5 2.7 260 0.94
total 778270 100 1 5349 1 52444 194.69
C15:0 1 116392 I
3:6-16
weightfeces
9.6
mg
area % Mgfatty ngfattyacid nmolfattyacid/
acid mgfeces mgfeces
C14:0
11600 1.2
10.6 1109
4.86
C16:0 381872 39.5 350.5 36507 142.38
C16:1 3071 0.3 2.8 294 1.15
C18:0 510655 52.8 468.7 48818 171.59
C18:1
50174 5.2
46.0 4797
16.98
C18:2 2963 0.3 2.7 283 1.01
C18:3 6403 0.7 5.9 612 2.20
total 966738 100 1 8872 1 92420 340.17
C15:0 1 87169 1
3:6-17
weightfeces
7.6
mg
area % Mgfatty ngfattyacid nmolfattyacid/
acid mgfeces mgfeces
C14:0 6657 1.8 6.5 849 3.72
C16:0 174284 45.8 168.9 22222 86.67
C16:1
1200 0.3 1.2 153
0.60
C18:0 175484 46.1 170.0 22375 78.65
C18:1
19588 5.2
19.0 2498
8.84
C18:2 1351 0.4 1.3 172 0.61
C18:3 1768 0.5 1.7 225 0.81
total 380332 1 11 3087 1 48494 T 179.90
C15:0 1 82557 1
3:6-18
weightfeces
8.1
mg
area % Mgfatty ngfattyacid nmolfattyacid/
acid mgfeces mgfeces
C14:0
8700 1.3
8.3 1022
4.47
C16:0 277658 41.8 264.1 32606 127.17
C16:1 1872 0.3 1.8 220 0.86
C18:0 334779 50.4 318.4 39313 138.18
C18:1
34667 5.2
33.0 4071
14.41
C18:2 1792 0.3 1.7 210 0.75
C18:3 4339 0.7 4.1 510 1.83
total 1 663807 T 1 6314 1 77952 T 287.68
C15:0 1 84105 1
Page A115 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:7-19
w e ig h t feces
10.7
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
9334 205542
1575 189750 23168
1823 1777
%
2.2 4 7 .5 0.4 4 3 .8 5.4 0.4 0.4
Mg fa tty acid
6.0 1 3 1 .2
1.0 121.1 14.8
1.2 1.1
ng fa tty acid m g feces 557 12263 94 11321 1382 109 106
nm ol fa tty acid/ m g feces 2 .4 4 4 7 .8 3 0.37 3 9 .7 9 4 .8 9 0 .3 9 0 .3 8
to ta l
I 432969 I 100
| 276 .4 | 25831 |
96.09
C 1 5:0
I 125320 1
2:7-19
w e ig h t feces
12.4
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
10569 262993
1907 260336 26561
1861 2435
%
1.9 4 6 .4 0.3 4 5 .9 4.7 0.3 0.4
Mg fa tty acid 8.5 2 1 2 .5 1.5 2 1 0 .3 2 1 .5 1.5 2.0
ng fa tty acid mg feces 689 17137 124 16963 1731 121 159
nm ol fatty acid/ mg feces 3.02 6 6 .8 4 0 .4 9 5 9 .6 3 6 .1 3 0 .4 3 0.57
to ta l
I 566662 |
100
| 457.9 | 369 24 |
1 3 7 .0 9
|
C 1 5:0 I 99012 1
I 1:7-20
w e ig h t fe ce s
7.2
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
5300 116241
798 106821 14034
1332 1059
%
2.2 4 7 .3 0.3 4 3 .5 5.7 0.5 0.4
Mg fa tty acid
3.9 86.1 0.6 79.1 10.4 1.0 0.8
ng fa tty acid m g feces 545 11953 82 10984 1443 137 109
nm ol fa tty acid/ m g feces 2 .3 9 4 6 .6 2 0.32 38.61 5.11 0 .4 9 0 .3 9
to ta l
I 245585 I 100
| 181.8 | 25 2 5 4 |
9 3 .9 2
C 1 5:0
I 108053 1
I 2:7-20
w e ig h t fe ce s
6
mg
I
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
4357 90363
573 77962 9578
799 815
%
2.4 4 9 .0 0.3 4 2 .3 5.2 0.4 0.4
Mg fa tty acid 3.7 75.7 0.5 6 5 .3 8.0 0.7 0.7
ng fa tty acid mg feces 608 12617 80 10886 1337 112 114
nm ol fatty acid/ mg feces 2 .6 6 49.21 0.31 3 8 .2 6 4 .7 3 0 .4 0 0.41
to ta l
I 184447 I
100
| 154.5 | 25 7 5 4 |
9 5 .9 9
C 1 5:0 I 954 90 1
I 1:7-21
w e ig h t fe ce s
10
mg
I 2:7-21
w e ig h t fe ce s
8.7
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
to ta l
area
%
9522 270616
2338 313018 37006
3195 3428
1.5 4 2 .3 0.4 4 9 .0 5.8 0.5 0.5
1 639123 T 100
Mg fa tty acid 6.2 1 7 6 .8 1.5 2 0 4 .6 24.2 2.1 2.2
1 4177
ng fa tty acid nm ol fa tty a c id /
m g feces
m g feces
622 2.72
17684
6 8 .9 7
153 0.60
20455
7 1 .9 0
2418
8 .5 6
209 0.74
224 0.80
r1 4 7 7 6 6
5 4 3
1
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
to ta l
area
7650 212450
1476 217370 22360
1545 2300
465151
%
1.6 45.7 0.3 46.7 4.8 0.3 0.5
100
C 15:0
1 122421 I
C 15:0
1 109572 I
Mg fa tty acid 5.6 155.1 1.1 1 5 8 .7 16.3 1.1 1.7
ng fa tty acid mg feces 642 17829 124 18242 1876 130 193
nm ol fatty acid/ mg feces 2.81 6 9 .5 4 0 .4 9 6 4 .1 2 6 .6 4 0 .4 6 0 .6 9
339 6 1 39036
1 4 4 .7 5
Page A116 of A172
Appendix to TNO-report of 3M-02
Final version
| 3:7-19 w e ig h t fe ce s
5 .8
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
4977 125240
723 123021 11441
691 1184
%
1.9 4 6 .9 0.3 4 6 .0 4.3 0.3 0.4
Mg fa tty acid 4.8 120.0 0.7 1 1 7 .8 1 1 .0 0.7 1.1
ng fa tty acid mg feces 822 20682 119 20316 1889 114 196
nm ol fa tty acid/ m g feces 3 .6 0 8 0 .6 6 0.47 71.41 6 .6 9 0.41 0 .7 0
to ta l
| 267277 |
100
| 256 .0 | 441 38 |
1 6 3 .9 4
|
C 15:0
| 83524 1
| 3:7-20 w e ig h t fe ce s
7.7
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
5155 125067
811 121012 14616
1143 1266
%
1.9 4 6 .5 0.3 4 5 .0 5.4 0.4 0.5
Mg fa tty acid 4.2 1 0 2 .5 0.7 99.1 1 2 .0 0.9 1.0
ng fa tty acid mg feces 548 13306 86 12875 1555 122 135
nm ol fa tty acid/ m g feces 2 .4 0 5 1 .9 0 0 .3 4 4 5 .2 5 5 .5 0 0 .4 3 0 .4 8
to ta l
| 269070 |
100
| 220 .4 | 28627 |
106.31
|
C 15:0
| 97655 1
| 3:7-21 w e ig h t fe ce s
17
mg
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
area
11944 374593
3142 460518 44662
3197 5430
%
1.3 4 1 .5 0.3 5 1 .0 4.9 0.4 0.6
Mg fa tty acid
10.3 3 2 1 .7
2.7 3 9 5 .5 3 8 .4
2.7 4.7
ng fa tty acid mg feces 603 18926 159 23268 2257 162 274
nm ol fa tty acid/ m g feces 2 .6 4 7 3 .8 2 0.62 8 1 .7 8 7 .9 9 0 .5 8 0 .9 9
to ta l
| 903486 |
100
| 776 .0 | 456 48 |
1 6 8 .4 ~
C 15:0
| 93140 1
04-12-2006
Page A117 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A118 of A172
group
i i
i
AVERAGE SD 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3
AVERAGE SD 4 4 4 4 4 4 4 4 4
AVERAGE SD 5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6
AVERAGE SD 7 7 7 7 7 7 7 7 7
AVERAGE SD
cage#
1,2 3 ,4
5 1,2 3 ,4 5 1,2 3 ,4 5
GC sam ple#
1:1-1 1 :1 -2 1 :1 -3 2 :1 -1 2 :1 -2 2 :1 -3 3 :1 -1 3 :1 -2 3 :1 -3
C 14:0 7 .0 9 6 .4 2 7 .0 9 4 .3 5 5 .1 3 5 .5 2 4 .4 8 5 .6 3 5 .0 3
fatty acids (pm ol/g feces)
C 1 6 :0 C 16:1 C 1 8 :0 C 18:1 C 1 8 :2
122 .2 3 1.06 9 9 .2 9 9.91 0 .5 6
141 .5 3 1.18 129.95 12.01 0 .8 0
160.62 1.46 156.30 16.90 0 .9 3
9 9 .4 9 0 .7 6 9 1 .4 2 10.51 0 .7 3
120.96 0 .8 7 106.39 10.83 0 .7 4
142.70 1.38 157.83 16.00 1.03
114.01 0 .9 8 163.82 17.82 1.05
1 4 2 .7 4 1 2 8 .9 5
1 .0 9 1 .0 2
142.89 15.61 138.45 13.83
0 .9 6 0 .9 0
C 18:3 1 .2 0 0 .3 5 1 .8 9 1 .1 5 1 .1 9 2 .1 0 2 .8 0 1 .6 7 1 .8 8
fatty acids total (pm ol/g feces)
2 4 1 .3 3 2 9 2 .2 4 3 4 5 .1 8 2 0 8 .4 0 2 4 6 .1 1 3 2 6 .5 6 3 0 4 .9 6 3 1 0 .5 9 2 9 0 .0 7
total feces
(g) 3 .0 7 5 2 .4 8 9 7 2 .2 9 4 6 4 .6 9 2 3 .7 7 8 9 3 .4 1 0 6 3 .5 7 5 4 2 .8 7 8 1 2 .7 5 8 9
average w eight days
(g) 1 2 3 .6 8 7 .2 5
2 2
8 8 .5 5 1 2 2 .4 5 8 6 .5 5
8 8 .2 1 2 1 .6 5
2 3 3 3 2
8 6 .5 5 8 8 .0 5
2 2
6 7 ,8 9 ,1 0
6 7 ,8 9 ,1 0
6 7 ,8 9 ,1 0
1 :2 -4 1 :2 -5 1 :2 -6 2 :2 -4 2 :2 -5 2 :2 -6 3 :2 -4 3 :2 -5 3 :2 -6
6 .7 6 9.11 3 .1 9 6.91 6 .9 0 3.61 7 .6 8 6 .6 3 2 .5 6
1 9 0 .3 7 2 3 6 .1 9 7 3 .7 3 1 6 1 .5 5 1 8 6 .4 0 5 5 .6 6 157.11 1 8 0 .7 8 6 9 .0 7
1 .7 5 0 .0 2 0 .6 5 1 .4 5 1 .8 4 0 .4 3 1 .3 7 1 .8 3 0 .5 6
2 3 0 .0 5 2 6 1 .5 9 6 9 .1 4 1 7 3 .5 0 2 2 4 .0 2 4 6 .9 8 1 8 3 .6 8 2 1 2 .1 8 7 2 .4 7
2 1 .5 2 3 4 .2 1 7 .0 4 1 8 .0 7 2 6 .3 9 5 .3 5 1 9 .1 6 2 3 .0 9 7 .7 1
1 .0 6 1 .7 3 0 .4 7 0 .9 4 1 .3 3 0 .4 3 1 .0 2 1 .2 0 0 .5 7
3 .4 0 3 .7 9 0 .7 9 2 .1 5 3 .2 4 0 .5 0 2 .4 2 2 .9 3 0 .8 7
4 5 4 .9 2 5 4 6 .6 4 155.01 3 6 4 .5 8 4 5 0 .1 2 1 1 2 .9 7 3 7 2 .4 5 4 2 8 .6 5 1 5 3 .8 0
2 .7 6 1 9 4 .0 5 8 1 2 .0 9 3 5 4 .0 7 5 6 .3 7 1 3 .4 0 7 4 2 .9 2 7 9 4 .3 9 8 5 2 .4 5 7 5
9 4 .5 5 1 2 6 .6 8 2 .2 9 4 .2 5 1 2 6 .4 5 82.1
94 1 2 6 .6 8 1 .8 5
2 2 2 3 3 3 2 2 2
1 1 ,1 2 ,1 3 15
1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7
1 :3 -7 1 :3 -8 1 :3 -9 2 :3 -7 2 :3 -8 2 :3 -9 3 :3 -7 3 :3 -8 3 :3 -9
4 .0 0 4 .6 5 4 .7 3 3 .6 5 5 .6 9 4 .4 4 3 .3 0 4 .3 2 5 .5 2
8 7 .1 6 1 2 3 .0 4 1 3 6 .8 9 8 3 .1 8 1 3 8 .5 3 1 1 1 .1 5 7 5 .3 2 1 0 3 .3 0 150.11
0 .7 6 1 .1 7 1 .2 9 0 .6 7 1 .2 5 1 .0 0 0 .5 6 0 .9 7 1 .0 7
8 1 .7 9 1 3 9 .9 5 1 7 1 .9 7 7 8 .9 6 1 4 4 .5 0 1 1 3 .6 3 7 2 .1 2 1 0 0 .6 3 1 7 2 .0 6
8 .1 3 1 3 .2 7 1 5 .8 7 7 .8 8 15.61 1 0 .9 8 7 .6 5 1 0 .7 5 19.51
0 .5 4 0 .8 1 1 .0 9 0 .5 6 0 .9 4 0 .7 7 0 .4 9 0 .6 0 1 .2 4
1 .0 0 1 .8 9 2.41 0.91 1 .7 7 1 .1 5 0 .9 3 1 .1 9 2 .2 4
1 8 3 .3 8 2 8 4 .7 7 3 3 4 .2 6 1 7 5 .8 0 3 0 8 .2 9 2 4 3 .1 2 1 6 0 .3 6 2 2 1 .7 6 3 5 1 .7 6
3 .4 5 9 7 2 .2 9 9 4 2 .3 4 5 4 5 .8 1 4 7 3 .7 7 1 8 3 .7 7 2 7 4 .0 2 5 2 .5 2 6 5 2 .6 9 0 5
118 8 3 .5 5 8 2 .8 1 1 8 .7 5 8 3 .5 8 3 .0 5 1 1 9 .6 8 4 .2 8 3 .4 5
2 2 2 3 3 3 2 2 2
18 19 2 0 ,2 1 18 19 2 0 ,2 1 18 19 2 0 ,2 1
1 :4 -1 0 1 :4 -1 1 1 :4 -1 2 2 :4 -1 0 2 :4 -1 1 2 :4 -1 2 3 :4 -1 0 3 :4 -1 1 3 :4 -1 2
5.91 4 .2 3 5 .5 2 5 .2 3 5 .1 2 5 .5 8 4 .8 7 7 .8 3 5 .6 2
1 6 2 .5 8 1 0 7 .9 6 150.11 152.71 1 3 3 .5 3 1 3 5 .0 5 1 3 2 .3 9 146.11 1 6 6 .5 5
1 .2 3 0 .8 9 1 .0 7 1 .1 6 1 .1 2 1 .0 3 1 .0 5 1 .2 7 1.41
1 7 7 .1 7 1 1 2 .7 9 1 7 2 .0 6 1 7 4 .9 4 1 4 0 .4 3 1 3 0 .5 8 1 5 1 .4 9 1 7 6 .3 5 2 1 2 .1 4
1 6 .9 0 1 0 .8 6 19.51 1 6 .1 7 1 4 .0 9 1 4 .9 8 1 3 .4 6 16.41 2 0 .6 3
1 .0 0 0 .6 7 1 .2 4 1 .0 5 0 .9 5 1 .0 5 0 .8 8 0 .9 2 1 .3 2
2 .2 7 1 .3 8 2 .2 4 2 .1 6 1 .5 9 1 .3 6 1 .8 0 2 .3 9 2 .8 5
3 6 7 .0 6 2 3 8 .7 8 3 5 1 .7 6 3 5 3 .4 4 2 9 6 .8 4 2 8 9 .6 3 3 0 5 .9 4 3 5 1 .2 8 4 1 0 .5 1
1 .6 8 9 3 .1 4 3 2 3 .2 5 4 5 2 .7 1 3 3 4 .6 4 0 7 5 .2 0 7 5 2 .0 4 1 3 .4 4 6 4 3 .9 5 4 9
5 9 .5 1 1 6 .4 1 1 2 .2 5 8 .8 5 1 1 6 .5 5 1 1 1 .8 5 5 8 .7 1 1 7 .4 5 1 1 2 .9
2 2 2 3 3 3 2 2 2
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
1 :5 -1 3 1 :5 -1 4 1 :5 -1 5 2 :5 -1 3 2 :5 -1 4 2 :5 -1 5 3 :5 -1 3 3 :5 -1 4 3 :5 -1 5
4 .9 9 7 .8 4 3 .8 7 5 .8 3 7 .5 8 3 .6 5 6 .7 3 8 .4 9 2 .5 6
1 2 9 .3 4 2 0 9 .9 5 9 1 .6 5 1 4 9 .2 8 2 0 0 .3 1 5 9 .2 4 1 5 4 .4 0 2 0 8 .7 9 5 9 .2 8
1 .0 0 2 .0 4 0 .7 7 1 .2 7 1 .9 7 0 .4 7 1 .3 5 2 .0 5 0 .4 4
1 4 0 .4 1 2 4 4 .5 4 8 8 .5 2 1 5 2 .2 9 2 1 8 .4 9 4 4 .3 7 1 8 7 .1 1 2 0 4 .2 2 5 7 .2 3
13.41 3 0 .4 7 8 .0 3 1 6 .0 3 2 7 .9 7 4 .9 9 1 9 .7 5 2 5 .1 4 6 .1 5
0 .9 7 1 .4 6 0 .4 6 0 .8 6 1.31 0 .3 2 1 .0 2 1 .3 3 0 .4 3
1 .6 8 3 .6 5 0 .9 6 1 .7 9 2 .7 6 0 .4 8 2 .6 4 2 .4 0 0 .6 5
2 9 1 .8 1 4 9 9 .9 6 1 9 4 .2 7 3 2 7 .3 5 4 6 0 .3 8 1 1 3 .5 3 3 7 3 .0 1 4 5 2 .4 2 1 2 6 .7 3
3 .1 9 5 2 .9 4 0 8 2 .6 3 9 9 4 .9 7 1 2 4 .4 8 3 3 3 .7 6 7 3 .6 1 1 3 3 .3 5 2 2 2 .7 0 3 4
1 1 0 .7 8 8 .3 5 8 4 .6 1 1 1 .6 8 8 .5 8 5 .5 5 1 1 2 .8 5 8 8 .9 8 5 .5
2 2 2 3 3 3 2 2 2
2 8 ,2 9 31
3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2
1 :6 -1 6 1 :6 -1 7 1 :6 -1 8 2 :6 -1 6 2 :6 -1 7 2 :6 -1 8 3 :6 -1 6 3 :6 -1 7 3 :6 -1 8
4 .6 9 4.11 4.21 4 .5 0 3 .7 0 4 .0 6 4 .8 6 3 .7 2 4 .4 7
1 3 5 .9 2 1 0 9 .7 4 1 1 3 .7 6 1 2 0 .7 7 7 1 .6 4 9 5 .0 2 1 4 2 .3 8 8 6 .6 7 1 2 7 .1 7
1 .0 8 0 .9 3 0 .9 3 0 .9 2 0 .4 4 0 .7 3 1 .1 5 0 .6 0 0 .8 6
1 5 7 .5 4 1 1 5 .4 0 1 2 6 .1 8 1 2 5 .3 2 5 9 .2 7 8 4 .1 7 1 7 1 .5 9 7 8 .6 5 1 3 8 .1 8
1 4 .9 6 1 0 .9 6 1 3 .0 9 1 2 .4 4 6 .0 8 9 .2 6 1 6 .9 8 8 .8 4 14.41
0 .9 4 0 .6 6 0 .7 9 0 .8 3 0 .4 1 0 .5 1 1.01 0 .6 1 0 .7 5
2 .0 9 1 .4 2 1 .6 4 1 .3 7 0 .6 5 0 .9 4 2 .2 0 0.81 1 .8 3
3 1 7 .2 1 2 4 3 .2 3 2 6 0 .5 9 2 6 6 .1 6 1 4 2 .2 0 1 9 4 .6 9 3 4 0 .1 7 1 7 9 .9 0 2 8 7 .6 8
2 .5 0 3 6 2 .7 0 8 3 2 .3 5 5 4 3 .9 6 8 4 3 .9 6 7 8 3 .5 6 6 6 2 .9 0 7 6 3 .2 4 4 9 2 .7 6 4 3
87.1 1 1 2 .8 8 5 .4 8 7 .1 5 1 1 2 .4 5 8 4 .5 5 8 7 .6 5 1 1 1 .9 5 8 4 .6 5
2 2 2 3 3 3 2 2 2
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
1 :7 -1 9 1 :7 -2 0 1 :7 -2 1 2 :7 -1 9 2 :7 -2 0 2 :7 -2 1 3 :7 -1 9 3 :7 -2 0 3 :7 -2 1
2 .4 4 2 .3 9 2 .7 2 3 .0 2 2 .6 6 2.81 3 .6 0 2 .4 0 2 .6 4
4 7 .8 3 4 6 .6 2 6 8 .9 7 6 6 .8 4 4 9 .2 1 6 9 .5 4 8 0 .6 6 5 1 .9 0 7 3 .8 2
0 .3 7 0 .3 2 0 .6 0 0 .4 9 0 .3 1 0 .4 9 0 .4 7 0 .3 4 0 .6 2
3 9 .7 9 3 8 .6 1 7 1 .9 0 5 9 .6 3 3 8 .2 6 6 4 .1 2 7 1 .4 1 4 5 .2 5 8 1 .7 8
4 .8 9 5 .1 1 8 .5 6 6 .1 3 4 .7 3 6 .6 4 6 .6 9 5 .5 0 7 .9 9
0 .3 9 0 .4 9 0 .7 4 0 .4 3 0 .4 0 0 .4 6 0 .4 1 0 .4 3 0 .5 8
0 .3 8 0 .3 9 0 .8 0 0 .5 7 0.41 0 .6 9 0 .7 0 0 .4 8 0 .9 9
9 6 .0 9 9 3 .9 2 1 5 4 .3 0 1 3 7 .0 9 9 5 .9 9 1 4 4 .7 5 1 6 3 .9 4 106.31 168.41
1 .9 6 9 7 2 .0 7 9 8 2 .6 7 0 5 3 .4 4 7 7 3 .2 6 4 6 4 .3 0 5 9 2 .5 1 1 7 2 .4 3 6 9 3 .4 1 0 7
8 1 .3 5 8 2 .2 5 1 0 1 .0 5 8 2 .3 5 8 2 .7 5 1 0 1 .9 5 8 3 .5 8 3 .3 5 1 0 3 .2
2 2 2 3 3 3 2 2 2
total fa tty acid excretion (pm ol/100 g r m ouse/day)
3 0 0 .2 0 4 1 6 .9 5 4 4 7 .2 4 2 6 6 .1 8 3 5 8 .1 9 4 2 0 .9 2 4 4 8 .1 5 5 1 6 .4 2 4 5 4 .4 4 4 0 3 .1 9 8 0 .0 9 6 6 4 .4 3 8 7 6 .1 2 1 9 7 .4 0 5 2 5 .4 3 7 5 5 .9 6 1 5 6 .2 9 5 8 0 .0 5 7 4 4 .6 3 2 3 0 .8 9 5 2 5 .6 9 2 6 8 .5 8 2 6 8 .8 3 3 9 1 .8 6 4 7 3 .4 1 2 8 6 .9 4 4 6 4 .2 0 3 6 8 .1 4 2 6 9 .8 3 3 3 2 .7 0 5 6 7 .0 5 3 8 0 .3 3 1 0 3 .9 7 5 2 0 .9 7 3 2 2 .3 9 5 1 0 .1 6 5 4 3 .1 8 3 9 3 .9 7 4 4 9 .4 8 5 3 1 .8 8 5 1 5 .3 9 7 1 9 .0 0 5 0 0 .7 1 1 1 0 .0 9 4 2 1 .1 1 8 3 2 .0 8 3 0 3 .1 0 4 8 6 .0 6 7 7 7 .4 1 1 6 6 .6 3 5 9 6 .8 3 8 5 2 .9 7 2 0 0 .3 5 5 1 5 .1 7 2 6 5 .8 1 4 5 5 .9 0 2 9 2 .0 0 3 5 9 .3 7 4 0 3 .9 9 1 6 7 .2 5 2 7 3 .7 6 5 6 4 .2 2 2 6 0 .7 2 4 6 9 .7 2 3 6 0 .7 7 1 2 4 .4 7 1 1 6 .3 3 1 1 8 .7 5 2 0 3 .8 9 1 9 1 .3 2 1 2 6 .2 3 2 0 3 .7 9 2 4 6 .5 6 1 5 5 .4 1 2 7 8 .3 0 1 8 2 .2 9 5 7 .6 8
% o f fatty acids
1
C 1 4 :0 C 1 6 :0 C16:1 C 1 8 :0 C 18:1 C 1 8 :2 C 18:3
2.94 50.65 0.44
4 1 .1 4
4.11 0 .2 3 0.5 0
2.20 48.43 0.40
4 4 .4 7
4.11 0 .2 7 0.1 2
2.05 46.53 0.42
4 5 .2 8
4.90 0.27 0.55
2.09 47.74 0.36 2.09 49.15 0.35
4 3 .8 7 4 3 .2 3
5 .0 4 4 .4 0
0 .3 5 0 .3 0
0 .5 5 0 .4 8
1.69 43 .7 0 0.42 1.47 37 .3 9 0.32
4 8 .3 3 5 3 .7 2
4 .9 0 5 .8 4
0 .3 2 0 .3 4
0 .6 4 0 .9 2
1.81 4 5 .9 6 0 .3 5 1.74 44 .4 6 0.35
4 6 .0 0 4 7 .7 3
5 .0 3 4 .7 7
0 .3 1 0 .3 1
0 .5 4 0 .6 5
2.01 4 6 .0 0 0.3 8 0 .4 2 3.91 0.0 4
4 5 .9 7 3 .6 5
4 .7 9 0 .5 4
0 .3 0 0 .0 4
0 .5 5 0.21
1.49 41 .8 5 0.39
5 0 .5 7
4.73 0.23 0.75
1.67 43.21 0.00
4 7 .8 5
6.26 0.32 0.69
2.06 47.57 0.42 1.90 44.31 0.40
4 4 .6 0 4 7 .5 9
4 .5 4 4 .9 6
0 .3 0 0 .2 6
0.51 0 .5 9
1.53 41.41 0.41 3.19 49.27 0.38
4 9 .7 7 4 1 .5 9
5 .8 6 4 .7 4
0 .3 0 0 .3 8
0 .7 2 0 .4 5
2.06 42.18 0.37
4 9 .3 2
5.14 0.28 0.65
1.55 42 .1 7 0.43
4 9 .5 0
5.39 0.28 0.68
1.66 44.91 0.36
4 7 .1 2
5.01 0 .3 7 0.5 6
1.90 44 .1 0 0.35
4 7 .5 4
5.18 0.30 0.62
0.53 2.74 0.13
2 .8 6
0.57 0.05 0.10
2 .1 8 4 7 .5 3 0.41
4 4 .6 0
4.43 0.30 0.54
1.63 43.21 0.41 1.42 40 .9 5 0.39
4 9 .1 4 5 1 .4 5
4 .6 6 4 .7 5
0 .2 8 0 .3 3
0 .6 6 0 .7 2
2 .0 8 47.31 0.38 1.85 4 4 .9 3 0.41
4 4 .9 1 4 6 .8 7
4 .4 8 5 .0 6
0 .3 2 0 .3 0
0 .5 2 0 .5 7
1.83 4 5 .7 2 0.41
4 6 .7 4
4.52 0.32 0.48
2.06 46.97 0.35
4 4 .9 7
4 .7 7 0.31 0.5 8
1.95 46 .5 8 0.44 1.57 4 2 .6 8 0.31
4 5 .3 8 4 8 .9 2
4 .8 5 5 .5 5
0 .2 7 0 .3 5
0 .5 3 0 .6 4
1.84 45 .1 0 0.39 0.26 2.33 0.04
4 7 .0 0 2 .3 7
4 .7 9 0 .3 5
0 .3 1 0 .0 2
0 .5 8 0 .0 8
1.61 4 4 .2 9 0 .3 4 1.77 45.21 0.37
4 8 .2 7 4 7 .2 4
4 .6 0 4 .5 5
0 .2 7 0 .2 8
0 .6 2 0 .5 8
1.57 4 2 .6 8 0.31 1.48 43.21 0.33
4 8 .9 2 4 9 .5 0
5 .5 5 4 .5 8
0 .3 5 0 .3 0
0 .6 4 0.61
1.73 44 .9 9 0.38 1.93 46 .6 3 0.36
4 7 .3 1 4 5 .0 9
4 .7 5 5 .1 7
0 .3 2 0 .3 6
0 .5 4 0 .4 7
1.59 43 .2 7 0.34
4 9 .5 2
4.40 0.29 0.59
2.23 41.59 0.36
5 0 .2 0
4.67 0.26 0.68
1.37 40 .5 7 0.34
5 1 .6 8
5.02 0.32 0.69
1.70 43 .6 0 0.35
4 8 .6 3
4.81 0.31 0.6 0
0 .2 6 1.89 0.02
1 .9 3
0.37 0.03 0.07
1.71 4 4 .3 2 0 .3 4
4 8 .1 2
4.60 0.33 0.58
1.57 4 1 .9 9 0.41
4 8 .9 1
6.09 0.29 0.73
1.99 47 .1 8 0.40
4 5 .5 6
4.13 0.24 0.50
1.78 45 .6 0 0.39
4 6 .5 2
4.90 0.26 0.55
1.65 43.51 0.43
4 7 .4 6
6.07 0.28 0.60
3.21 5 2 .1 8 0.4 2
3 9 .0 9
4.40 0.28 0.42
1.81 4 1 .3 9 0 .3 6
5 0 .1 6
5 .3 0 0 .2 7 0.71
1.88 46 .1 5 0.45 2.02 46.77 0.34
4 5 .1 4 4 5 .1 6
5 .5 6 4 .8 6
0 .2 9 0 .3 4
0 .5 3 0.51
1.96 45 .4 6 0.39
4 6 .2 4
5.10 0.29 0.57
0.49 3.24 0.04
3 .1 9
0.70 0.03 0.10
1.48 42 .8 5 0.34
4 9 .6 6
4.72 0.30 0.66
1.69 45 .1 2 0.38
4 7 .4 4
4.51 0 .2 7 0.5 9
1.62 43 .6 5 0.36
4 8 .4 2
5.02 0.30 0.63
1.69 45 .3 8 0.35
4 7 .0 8
4 .6 8 0.31 0.5 2
2 .6 0 5 0 .3 8 0.31 2 .0 9 48.81 0.37
4 1 .6 8 4 3 .2 3
4 .2 8 4 .7 6
0 .2 9 0 .2 6
0 .4 5 0 .4 8
1.43 41 .8 6 0.34
5 0 .4 4
4.99 0.30 0.65
2.07 48.18 0.33 1.55 44 .2 0 0.30
4 3 .7 2 4 8 .0 3
4 .9 1 5 .0 1
0 .3 4 0 .2 6
0 .4 5 0 .6 4
1.80 45 .6 0 0.34
4 6 .6 4
4.76 0.29 0.56
0.38 2.90 0.03
3 .0 5
0.25 0.03 0.09
2.54 49.77 0.38
4 1 .4 1
5.09 0.40 0.40
2.54 49.63 0.34 1.77 44 .7 0 0.39
4 1 .1 1 4 6 .6 0
5 .4 4 5 .5 5
0 .5 2 0 .4 8
0 .4 2 0 .5 2
2.20 48.75 0.36
4 3 .4 9
4.47 0.32 0.42
2.77 51.26 0.33
3 9 .8 6
4 .9 3 0.41 0.4 3
1.94 48 .0 4 0.34
4 4 .3 0
4.59 0.32 0.48
2.20 49.20 0.29
4 3 .5 6
4.08 0.25 0.43
2 .2 6 48.81 0.32
4 2 .5 7
5 .1 8 0.41 0.4 5
1.57 43 .8 3 0.37
4 8 .5 6
4.74 0.34 0.58
2.20 48.22 0.35
4 3 .4 9
4.90 0.38 0.46
0.39 2.42 0.03
2 .7 4
0.48 0.09 0.06
Appendix to TNO-report of 3M-02
Final version
04-12-2006
fatty acid
total fatty acids C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
fatty acid
total fatty acids C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
fatty acid
total fatty acids C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
group 1 vs 2 nn p 9 9 0.258 9 9 0.297 9 9 0.136 9 9 0.666 9 9 0.191 9 9 0.222 9 9 0.931 9 9 0.190 group 2 vs 4
nn p 9 9 0.489 9 9 0.546 9 9 1.000 9 9 0.019 9 9 0.489 9 9 0.190 9 9 0.863 9 9 0.546 group 3 vs 7 nn p 9 9 <0.001 9 9 0.040 9 9 0.011 9 9 0.019 9 9 0.006 9 9 0.546 9 9 0.031 9 9 0.002
group 1 vs 3 nn p 9 9 0.730 9 9 0.436 9 9 0.340 9 9 0.730 9 9 0.297 9 9 0.863 9 9 0.666 9 9 0.666
group 2 vs 5 nn p 9 9 1.000 9 9 0.605 9 9 0.436 9 9 0.730 9 9 0.340 9 9 0.730 9 9 0.489 9 9 0.297 group 4 vs 5 nn p 9 9 1.000 9 9 0.113 9 9 0.161 9 9 0.019 9 9 0.094 9 9 0.387 9 9 0.297 9 9 0.340
fatty acid excretion, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5 group 1 vs 6
nn E nn p nn
p
9 9 0.040 9 9 0.387 9 9 0.546
9 9 0.094 9 9 0.436 9 9 0.190 9 9 0.050 9 9 0.436 9 9 0.489 9 9 0.136 9 9 0.605 9 9 0.077
9 9 0.050 9 9 0.546 9 9 0.546 9 9 0.931 9 9 0.387 9 9 0.863 9 9 0.863 9 9 0.387 9 9 0.387
9 9 0.387 9 9 0.863 9 9 1.000
group 2 vs 6 group 2 vs 7 group 3 vs 4
nn p nn p nn
p
9 9 0.222 9 9 0.008 9 9 0.040
9 9 0.931 9 9 0.063 9 9 0.222
9 9 0.222 9 9 0.008 9 9 0.190
9 9 0.014 9 9 0.040 9 9 0.014
9 9 0.546 9 9 0.008 9 9 0.077
9 9 0.094 9 9 0.436 9 9 1.000
9 9 0.796 9 9 0.024 9 9 0.730
9 9 0.190 9 9 0.001 9 9 0.436
group 4 vs 6 group 4 vs 7 group 5 vs 6
nn p nn p nn
p
9 9 0.040 9 9 <0.001 9 9 0.222
9 9 0.730 9 9 0.011 9 9 0.340
9 9 0.161 9 9 0.002 9 9 1.000
9 9 0.730 9 9 1.000 9 9 0.011
9 9 0.222 9 9 0.001 9 9 0.863
9 9 0.931 9 9 0.605 9 9 0.489
9 9 0.436 9 9 0.040 9 9 0.546
9 9 0.489 9 9 <0.001 9 9 1.000
group 1 vs 7 nn p 9 9 <0.001 9 9 0.222 9 9 0.136 9 9 0.136 9 9 0.136 9 9 0.605 9 9 0.019 9 9 0.031 group 3 vs 5 nn p 9 9 0.297 9 9 0.931 9 9 0.931 9 9 0.863 9 9 1.000 9 9 0.340 9 9 0.136 9 9 0.796 group 5 vs 7 nn p 9 9 0.004 9 9 0.136 9 9 0.050 9 9 0.019 9 9 0.063 9 9 0.605 9 9 0.011 9 9 0.014
group 2 vs 3 nn p 9 9 0.297 9 9 0.730 9 9 0.387 9 9 0.730 9 9 0.387 9 9 0.113 9 9 0.546 9 9 0.340
group 3 vs 6 nn p 9 9 0.730 9 9 0.666 9 9 0.796 9 9 0.008 9 9 1.000 9 9 0.863 9 9 0.161 9 9 0.666 group 6 vs 7 nn p 9 9 0.002 9 9 0.040 9 9 0.077 9 9 0.796 9 9 0.050 9 9 0.489 9 9 0.008 9 9 0.008
p<005
Page A119 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Fatty acid balance
group
1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2
AVERAGE SD
3 3 3 3 3 3 3 3 3
AVERAGE SD
4 4 4 4 4 4 4 4 4
AVERAGE SD
5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6
AVERAGE SD
7 7 7 7 7 7 7 7 7
AVERAGE SD
cage#
1,2 3,4 5 1,2 3,4 5 1,2 3,4 5
GC sam ple#
1:1-1 1:1 -2 1:1-3 2:1-1 2:1-2 2:1-3 3:1-1 3:1-2 3:1-3
6 7,8 9,10 6 7,8 9,10 6 7,8 9,10
1:2-4 1:2-5 1:2 -6 2:2-4 2:2-5 2:2-6 3:2-4 3:2-5 3:2-6
1 1 ,1 2 ,1 3 15
1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7 1 1 ,1 2 ,1 3
15 1 4 ,1 6 ,1 7
1:3-7 1:3-8 1:3-9 2:3-7 2:3-8 2:3-9 3:3-7 3:3-8 3:3-9
18 19 20,21 18 19 20,21 18 19 20,21
1:4-10 1:4-11 1:4-12 2 :4 -1 0 2:4-11 2 :4 -1 2 3 :4 -1 0 3:4-11 3 :4 -1 2
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
22 2 4 ,2 5 2 3 ,2 6 ,2 7
1:5-13 1:5-14 1:5-15 2 :5 -1 3 2 :5 -1 4 2 :5 -1 5 3 :5 -1 3 3 :5 -1 4 3 :5 -1 5
2 8 ,2 9 31
3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2 2 8 ,2 9
31 3 0 ,3 2
1:6-16 1:6-17 1:6-18 2 :6 -1 6 2 :6 -1 7 2 :6 -1 8 3 :6 -1 6 3 :6 -1 7 3 :6 -1 8
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
3 3 ,3 4 ,3 5 3 6 ,3 7 3 8 ,3 9
1:7-19 1:7-20 1:7-21 2 :7 -1 9 2 :7 -2 0 2:7-21 3 :7 -1 9 3 :7 -2 0 3:7-21
total fatty acids (pm ol/g feces)
2 4 1 .3 3 2 9 2 .2 4 3 4 5 .1 8 2 0 8 .4 0 246.11 3 2 6 .5 6 3 0 4 .9 6 3 1 0 .5 9 2 9 0 .0 7
4 5 4 .9 2 5 4 6 .6 4 155.01 3 6 4 .5 8 4 5 0 .1 2 1 1 2 .9 7 3 7 2 .4 5 4 2 8 .6 5 1 5 3 .8 0
1 8 3 .3 8 2 8 4 .7 7 3 3 4 .2 6 1 7 5 .8 0 3 0 8 .2 9 2 4 3 .1 2 1 6 0 .3 6 2 2 1 .7 6 3 5 1 .7 6
3 6 7 .0 6 2 3 8 .7 8 3 5 1 .7 6 3 5 3 .4 4 2 9 6 .8 4 2 8 9 .6 3 3 0 5 .9 4 3 5 1 .2 8 410.51
291.81 4 9 9 .9 6 1 9 4 .2 7 3 2 7 .3 5 4 6 0 .3 8 1 1 3 .5 3 373.01 4 5 2 .4 2 1 2 6 .7 3
317.21 2 4 3 .2 3 2 6 0 .5 9 2 6 6 .1 6 1 4 2 .2 0 1 9 4 .6 9 3 4 0 .1 7 1 7 9 .9 0 2 8 7 .6 8
9 6 .0 9 9 3 .9 2 1 5 4 .3 0 1 3 7 .0 9 9 5 .9 9 1 4 4 .7 5 1 6 3 .9 4 106.31 168.41
total feces (g)
3 .0 7 5 2.4 8 9 7 2.2 9 4 6 4 .6 9 2 3.7 7 8 9 3.4 1 0 6 3.5 7 5 4 2.8781 2.7 5 8 9
2.7 6 1 9 4.0581 2.0 9 3 5 4 .0 7 5 6.371 3.4 0 7 4 2.9 2 7 9 4.3 9 8 5 2.4 5 7 5
3.4 5 9 7 2.2 9 9 4 2.3 4 5 4 5.8 1 4 7 3.7 7 1 8 3.7 7 2 7 4 .0 2 5 2.5 2 6 5 2.6 9 0 5
1 .6 8 9 3.1 4 3 2 3.2 5 4 5 2.7 1 3 3 4.6 4 0 7 5.2 0 7 5 2.041 3.4 4 6 4 3.9 5 4 9
3 .1 9 5 2.9 4 0 8 2.6 3 9 9 4.9 7 1 2 4.4 8 3 3 3 .7 6 7 3.6 1 1 3 3.3 5 2 2 2.7 0 3 4
2.5 0 3 6 2.7 0 8 3 2.3 5 5 4 3.9 6 8 4 3.9 6 7 8 3.5 6 6 6 2.9 0 7 6 3.2 4 4 9 2.7 6 4 3
1 .9 6 9 7 2.0 7 9 8 2.6 7 0 5 3.4 4 7 7 3.2 6 4 6 4.3 0 5 9 2.5 1 1 7 2.4 3 6 9 3.4 1 0 7
average weight
(g) 1 2 3 .6 8 7 .2 5 8 8 .5 5 1 2 2 .4 5 8 6 .5 5 88.2 1 2 1 .6 5 8 6 .5 5 8 8 .0 5
days
2 2 2 3 3 3 2 2 2
9 4 .5 5 1 2 6 .6 82.2 9 4 .2 5 1 2 6 .4 5 82.1
94 1 2 6 .6 8 1 .8 5
2 2 2 3
3
3 2 2 2
118 8 3 .5 5 82.8 1 1 8 .7 5 83.5 8 3 .0 5 1 1 9 .6 84.2 8 3 .4 5
2 2 2 3
3
3 2 2 2
59.5 1 1 6 .4 112.2 5 8 .8 5 1 1 6 .5 5 1 1 1 .8 5 58.7 1 1 7 .4 5 1 1 2 .9
2 2 2 3
3 3 2 2 2
1 1 0 .7 8 8 .3 5 84.6 111.6 88.5 8 5 .5 5 1 1 2 .8 5 88.9 85.5
2 2 2 3
3
3 2 2 2
87.1 112.8 85.4 8 7 .1 5 1 1 2 .4 5 8 4 .5 5 8 7 .6 5 1 1 1 .9 5 8 4 .6 5
2 2 2 3
3 3 2 2 2
8 1 .3 5 8 2 .2 5 1 0 1 .0 5 8 2 .3 5 8 2 .7 5 1 0 1 .9 5 83.5 8 3 .3 5 1 0 3 .2
2 2 2 3
3
3 2 2 2
fatty acids excretion (pm ol/100 gr m ouse/day)
3 0 0 .2 0 4 1 6 .9 5 4 4 7 .2 4 2 6 6 .1 8 3 5 8 .1 9 4 2 0 .9 2 4 4 8 .1 5 5 1 6 .4 2 4 5 4 .4 4 4 0 3 .1 9 8 0 .0 9
6 6 4 .4 3 8 7 6 .1 2 1 9 7 .4 0 5 2 5 .4 3 7 5 5 .9 6 1 5 6 .2 9 5 8 0 .0 5 7 4 4 .6 3 2 3 0 .8 9
5 2 5 .6 9 2 6 8 .5 8 2 6 8 .8 3 3 9 1 .8 6 473.41 2 8 6 .9 4 4 6 4 .2 0 3 6 8 .1 4 2 6 9 .8 3 3 3 2 .7 0 5 6 7 .0 5 3 8 0 .3 3 1 0 3 .9 7 5 2 0 .9 7 3 2 2 .3 9 5 1 0 .1 6 5 4 3 .1 8 3 9 3 .9 7 4 4 9 .4 8 5 3 1 .8 8 5 1 5 .3 9 7 1 9 .0 0 500.71 1 1 0 .0 9 421.11 8 3 2 .0 8 3 0 3 .1 0 4 8 6 .0 6 777.41 1 6 6 .6 3 5 9 6 .8 3 8 5 2 .9 7 2 0 0 .3 5 5 1 5 .1 7 265.81 4 5 5 .9 0 2 9 2 .0 0 3 5 9 .3 7 4 0 3 .9 9 1 6 7 .2 5 2 7 3 .7 6 5 6 4 .2 2 2 6 0 .7 2 4 6 9 .7 2 3 6 0 .7 7 1 2 4 .4 7
1 1 6 .3 3 1 1 8 .7 5 2 0 3 .8 9 1 9 1 .3 2 1 2 6 .2 3 2 0 3 .7 9 2 4 6 .5 6 155.41 2 7 8 .3 0 1 8 2 .2 9 5 7 .6 8
fo o d intake (g)
23.2 18
16.8 36.8 28.8 25.6 20.2 15.3 13.8
19.9 27.7 16.3 28.8 45.4 28.2 16.7 23.6 13.2
26.2 16.5 18.3 44.7 28.3 29.3 23.7 13.6 14.7
12 21.7 24 19.8 35.2 38.2 11.3 18.7 22.6
21.5 19.6 19.5 36.5 32.2 30.3 18.7 28 14.5
17.6 20.3 17.2 29.7
31 27.9 16.5 17.1
15
16.8 16.9 21.9 29.2 26.5 35 15.7
15 19.8
fa tty a c id s uptake (pm ol/100 gr m ouse/day)
5 2 8 3 .2 2 5 8 0 6 .7 9 5340.11 5 6 3 9 .3 2 6244.01 5 4 4 6 .4 0 4 6 7 3 .7 8 4 9 7 5 .6 9 4 4 1 1 .4 3
5 9 2 4 .0 8 6158.51 5 5 8 1 .4 2 5 7 3 3 .8 9 6 7 3 7 .1 3 6445.31 5 0 0 0 .5 5 5 2 4 6 .9 6 4 5 3 9 .2 5
6 2 4 9 .5 5 5 5 5 8 .6 2 6 2 2 0 .8 5 7 0 6 3 .3 7 6 3 5 9 .7 2 6 6 2 0 .1 2 5 5 7 7 .5 9 4 5 4 6 .2 8 4 9 5 8 .1 6
5 6 7 6 .6 7 5 2 4 7 .3 0 6020.71 6 3 1 3 .3 0 5 6 6 7 .2 0 6 4 0 8 .6 3 5 4 1 8 .3 8 4 4 8 1 .4 4 5 6 3 4 .3 5
5 4 6 6 .6 4 6 2 4 4 .2 3 6 4 8 7 .7 4 6 1 3 7 .1 5 6 8 2 7 .3 3 6 6 4 6 .0 0 4 6 6 4 .1 2 8 8 6 5 .1 4 4 7 7 3 .4 4
5 6 8 7 .5 3 5 0 6 5 .4 3 5668.91 6 3 9 4 .8 0 5 1 7 2 .9 7 6 1 9 1 .9 7 5 2 9 8 .6 0 4 2 9 9 .3 3 4 9 8 7 .6 2
5 8 1 2 .7 4 5 7 8 3 .3 6 6 1 0 0 .1 0 6653.61 6 0 0 9 .1 9 6 4 4 1 .9 7 5 2 9 2 .2 8 5065.41 5 4 0 0 .2 6
ch o le ste ro l balance (pm ol/100 gr mouse/day)
4 9 8 3 .0 2 5 3 8 9 .8 4 4 8 9 2 .8 7 5 3 7 3 .1 5 5 8 8 5 .8 2 5 0 2 5 .4 8 4 2 2 5 .6 4 4 4 5 9 .2 7 3 9 5 6 .9 8 4 9 1 0 .2 3 6 1 1 .9 3
5 2 5 9 .6 5 5 2 8 2 .3 9 5 3 8 4 .0 3 5 2 0 8 .4 5 5 9 8 1 .1 7 6 2 8 9 .0 3 4 4 2 0 .5 0 4 5 0 2 .3 3 4 3 0 8 .3 6
5 1 8 1 .7 7 6 8 2 .5 4 5 9 8 0 .7 1 5 1 6 6 .7 6 5 7 4 7 .4 5 6 7 7 6 .4 3 5 8 9 5 .5 2 6 2 5 1 .9 8 5 3 0 7 .7 6 4 2 1 3 .5 7 4 3 9 1 .1 1 5 5 2 5 .7 0 8 4 1 .8 8 5 1 5 5 .7 0 4 9 2 4 .9 1 5 5 1 0 .5 5 5 7 7 0 .1 3 5 2 7 3 .2 2 5 9 5 9 .1 5 4 8 8 6 .5 1 3 9 6 6 .0 5 4 9 1 5 .3 4 5 1 5 1 .2 8 587.51 5 0 4 5 .5 2 5 4 1 2 .1 5 6 1 8 4 .6 4 5 6 5 1 .0 9 6 0 4 9 .9 2 6 4 7 9 .3 8 4 0 6 7 .2 9 8 0 1 2 .1 6 4 5 7 3 .0 8 5 7 1 9 .4 7 1 1 5 9 .5 6 5 2 3 1 .6 3 4 7 7 3 .4 3 5 3 0 9 .5 4 5 9 9 0 .8 1 5 0 0 5 .7 2 5 9 1 8 .2 1 4 7 3 4 .3 8 4 0 3 8 .6 2 4 5 1 7 .9 0 5 0 5 7 .8 1 6 3 4 .7 0
5 6 9 6 .4 2 5 6 6 4 .6 1 5 8 9 6 .2 1 6 4 6 2 .2 9 5 8 8 2 .9 6 6 2 3 8 .1 8 5 0 4 5 .7 1 4 9 1 0 .0 0 5 1 2 1 .9 6 5 6 5 7 .5 9 5 3 7 .8 3
m olw
C 14:0 C 16:0 C 1 6 :1 C 18:0 C 1 8 :1 C 18:2 C 18:3
average
2 2 8 .4 2 5 6 .4 2 5 4 .4 2 8 4 .5 2 8 2 .5 2 8 0 .5 2 7 8 .5
M Y S TR IC A C ID P A LM ITIC A C ID P A LM IT O LE IC AC ID S T E A R IC A C ID O LE IC AC ID LIN O LE IC AC ID LIN O LE N IC AC ID
266.46
g/mol
diet: 15 % fat=
0 .5 6 3
m m ol per g diet
Page A120 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
fatty acid balance balance
fatty acid balance balance
fatty acid balance balance
fatty acid balance, p values Mann-Whitney U-test
group 1 vs 2 I group 1 vs 3 group 1 vs 4 group 1 vs 5 I group 1 vs 6
nn E nn p nn p
nn
p
nn
p
9 9 0.436 9 9 0.113
9 0.489 9 9 0.063 9 SH 0.730
group 2 vs 4 group 2 vs 5 group 2 vs 6 group 2 vs 7
group 3 vs 4
nn
n n p__ I n n p
n n p__ I n n
p
9 9 0.8631 9 91 0.258 I 9 9 0.7961 9 9 l 0.258 I 9 9 0.222
group 3 vs 7 group 4 vs 5 group 4 vs 6 group 4 vs 7
group 5 vs 6
n n p nn
p_ n n p n n
p__ n n
p
9 9 1.0001 9 9 l 0.222 9 9 0.730| 9 9 l 0.136 9 9 0.136
group 1 vs 7 group 2 vs 3
nn
nn p
r~9~ 9 0.019 9 9 0.546
group 3 vs 5 group 3 vs 6
rsn n p__ I n
r 9 l 91 0.863 I
ni
9]
p__ I
0.297 I
group 5 vs 7 group 6 vs 7
n n p nn
p_
9 9 0.931| 9 9 l 0.094
p<0.05
Page A121 of A172
Appendix to TNO-report of 3M-02
Final version
Appendix F. Intestinal cholesterol absorption
C holesterol absorption
O ral g a v a g e m ix:
14 C dpm average:
7 6 0 8 3 .3 7 6 5 4 7 .5 7 5 2 0 5 .8 7 4 1 7 4 .5 7 3 7 6 3 .5 7 5 6 6 9 .7 1 75241
I
3H dpm average:
92065.2 92196.7 90472.4 88575.4 92526.8 92548.9 91398 |
14C /3H dosing: 1
0.8231
Mouse# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
14C dpm feces 7900.00 9070.58 8759.79
7943.46 9442.29 9027.49
9996.01 10914.90 11495.20
8402.11 8966.49 9560.47
10754.70 11858.50 12382.30
8918.47 9795.44 9570.76
11342.40 10247.10 11420.10
10160.50 11533.30 11375.70
9360.62 9242.62 9409.35
10257.10 11572.10 11790.60
7215.69 6854.01 8420.46
7620.85 7592.86 7701.77
10718.20 9765.43 11045.10
8973.56 10118.90 10186.80
11415.30 11521.70 9926.39
9713.21 10522.10 10357.80
10622.10 10309.40 9363.58
9289.29 8492.80 9169.78
7720.92 8438.85 8631.31
10252.90 9662.34 10 5 8 9 .0 0
3H dpm feces 17857.0 20224.1 19669.7
AVERAGE 17369.9 20044.0 19314.7
AVERAGE 16226.5 17696.9 19154.0
AVERAGE 15319.6 16793.3 16635.3
AVERAGE 19111.4 20818.2 21352.2
AVERAGE 19316.9 21221.6 20766.7
AVERAGE 19935.8 17921.2 19737.7
AVERAGE 22122.3 24951.5 24849.9
AVERAGE 18676.0 18523.6 18338.6
AVERAGE 18752.4 20953.2 21592.7
AVERAGE 13209.8 12708.1 14817.4
AVERAGE 13562.2 13608.7 13685.9
AVERAGE 22219.9 20159.2 22642.2
AVERAGE 15531.3 17347.9 17603.2
AVERAGE 21059.0 21372.1 19181.1
AVERAGE 17538.7 18995.2 18714.0
AVERAGE 17065.5 16759.2 15186.6
AVERAGE 18158.2 16878.2 18096.8
AVERAGE 15528.4 17238.0 17527.6
AVERAGE 18655.8 17086.2 19019.0
A V E RAGE
14/3H feces 0.442 0.449 0.445 0.445 0.457 0.471 0.467 0.465 0.616 0.617 0.600 0.611 0.548 0.534 0.575 0.552 0.563 0.570 0.580 0.571 0.462 0.462 0.461 0.461 0.569 0.572 0.579 0.573 0.459 0.462 0.458 0.460 0.501 0.499 0.513 0.504 0.547 0.552 0.546 0.548 0.546 0.539 0.568 0.551 0.562 0.558 0.563 0.561 0.482 0.484 0.488 0.485 0.578 0.583 0.579 0.580 0.542 0.539 0.518 0.533 0.554 0.554 0.553 0.554 0.622 0.615 0.617 0.618 0.512 0.503 0.507 0.507 0.497 0.490 0.492 0.493 0.550 0.566 0.557 0.557
% cholesterol absorption 45.89 43.48 25.78 32.90 30.67 43.95 30.38 44.15 38.73 33.38 33.03 31.87 41.10 29.56 35.27 32.73 24.92 38.39 40.11 32.30
04-12-2006
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Appendix to TNO-report of 3M-02
Final version
Mouse# 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45
14C dpm feces 8632.76 7757.78 8732.12
9317.58 10349.10 10995.40
12726.80 11922.70 10824.40
11803.80 11509.60 10446.20
9824.33 10536.10 10819.00
9944.17 9896.95 9024.08
11012.10 12345.10 12611.60
10539.20 11997.90 11733.20
8467.17 8615.23 7791.76
10611.20 11669.60 11704.20
6797.41 8479.36 8042.25
9667.22 10332.00 10290.40
9274.67 9043.46 8359.95
8337.87 8978.72 9290.98
9260.73 8926.69 8216.57
7388.65 7801.01 8272.11
8775.47 8627.50 7935.44
8265.72 7672.71 7372.15
6512.96 7565.00 7444.81
8569.40 9540.76 9523.59
8330.02 9412.73 9427.18
9607.51 9889.25 9677.26
10990.90 9651.99 11648.90
9120.82 10887.20 11023.60
7559.63 8832.90 9369.62
3H dpm feces 18263.4 16543.7 18151.9
AVERAGE 18797.2 20750.2 21398.2
AVERAGE 21922.9 20859.7 19073.0
AVERAGE 20531.4 19930.8 17926.4
AVERAGE 17823.8 19638.0 19717.7
AVERAGE 17861.6 17830.0 16251.3
AVERAGE 20530.5 22826.4 23740.9
AVERAGE 19138.3 21840.1 21335.4
AVERAGE 19713.7 19837.0 18343.0
AVERAGE 16118.3 17565.2 17995.9
AVERAGE 15090.2 18363.5 17655.2
AVERAGE 18299.0 19525.1 19416.7
AVERAGE 18965.3 18555.8 17064.5
AVERAGE 16938.5 18142.5 18500.1
AVERAGE 21618.5 20655.8 19301.2
AVERAGE 17101.3 18082.3 19128.3
AVERAGE 18331.8 18170.1 16944.8
AVERAGE 17166.9 16099.9 15533.3
AVERAGE 13424.4 15510.5 15298.9
AVERAGE 17514.8 19514.6 19814.4
AVERAGE 16006.8 17737.5 17550.5
AVERAGE 19624.7 19941.0 19580.0
AVERAGE 20009.6 17422.8 20445.0
AVERAGE 17747.4 20878.9 21294.1
AVERAGE 14314.6 16604.8 17203. 4
AVERAGE
14/3H feces 0.473 0.469 0 .4 8 1 0.474 0.496 0.499 0.514 0.503 0 .5 8 1 0.572 0.568 0.573 0.575 0.577 0.583 0.578 0 .5 5 1 0.537 0.549 0.545 0.557 0.555 0.555 0.556 0.536 0 .5 4 1 0 .5 3 1 0.536 0 .5 5 1 0.549 0.550 0.550 0.430 0.434 0.425 0.430 0.658 0.664 0.650 0.658 0.450 0.462 0.456 0.456 0.528 0.529 0.530 0.529 0.489 0.487 0.490 0.489 0.492 0.495 0.502 0.496 0.428 0.432 0.426 0.429 0.432 0 .4 3 1 0.432 0.432 0.479 0.475 0.468 0.474 0 .4 8 1 0.477 0.475 0.478 0.485 0.488 0.487 0.487 0.489 0.489 0 .4 8 1 0.486 0.520 0 .5 3 1 0.537 0.529 0.490 0.496 0.494 0.493 0.549 0.554 0.570 0.558 0.514 0 .5 2 1 0.518 0.518 0.528 0.532 0.545 0.535
% cholesterol absorption 42.39 38.93 30.37 29.74 33.74 32.50 34.87 33.19 47.82 20.11 44.62 35.72 40.63 39.69 47.92 47.53 42.43 41.99 40.90 40.93 35.69 40.08 32.26 37.12 35.02
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Appendix to TNO-report of 3M-02
Final version
M ouse# 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70
14C dpm feces 13292.00 12025.90 8154.44
7073.99 8303.55 8312.14
8105.89 9177.91 8404.42
9083.21 10025.30 10551.50
7998.17 9589.24 9485.67
8469.79 9318.33 9497.10
8613.92 8699.27 7646.35
10299.70 9010.87 10286.90
8794.09 9327.04 9893.72
12083.00 11744.30 10287.20
7269.37 8544.20 8283.26
7909.58 7041.53 8328.59
7120.23 8146.99 8204.53
8975.84 9015.56 7523.90
7587.01 8719.17 8811.04
7582.08 8218.83 8867.68
8808.83 8584.87 7447.43
7758.45 6896.60 7801.31
8952.17 10058.20 10221.10
8171.80 7457.41 8538.29
8381.63 7890.69 7086.95
9800.20 11238.90 11334.00
6081.05 6823.98 6967.67
7436.52 8308.62 8710.60
8595.56 8773.69 7650.67
3H dpm feces 23039.1 20891.1 14093.8
AVERAGE 11977.8 13879.6 13866.1
AVERAGE 14355.8 16142.2 14482.0
AVERAGE 16293.9 17848.5 18335.8
AVERAGE 17522.8 20188.8 20422.8
AVERAGE 18261.6 19807.5 20101.5
AVERAGE 19823.8 19531.5 17499.6
AVERAGE 20630.3 17880.3 20622.3
AVERAGE 18229.9 19419.7 20500.1
AVERAGE 22066.1 21734.5 19073.9
AVERAGE 16991.1 19484.0 19099.1
AVERAGE 18224.2 16492.5 19376.2
AVERAGE 17677.8 19926.7 19132.5
AVERAGE 20407.6 19983.1 17563.4
AVERAGE 17288.6 19191.4 19513.2
AVERAGE 18143.7 20142.1 21451.9
AVERAGE 21295.7 20799.9 18297.0
AVERAGE 17723.8 15907.7 18207.4
AVERAGE 21000.2 23550.7 23744.5
AVERAGE 18430.1 17029.8 19260.2
AVERAGE 20381.9 19487.6 17369.0
AVERAGE 23428.2 26990.4 26923.6
AVERAGE 14094.2 15200.4 16000.0
AVERAGE 18241.1 20465.5 21554.3
AVERAGE 21140.4 2 13 9 0 .2 18677.5
AVERAGE
14/3H feces 0.577 0.576 0.579 0.577 0.591 0.598 0.599 0.596 0.565 0.569 0.580 0.571 0.557 0.562 0.575 0.565 0.456 0.475 0.464 0.465 0.464 0.470 0.472 0.469 0.435 0.445 0.437 0.439 0.499 0.504 0.499 0.501 0.482 0.480 0.483 0.482 0.548 0.540 0.539 0.542 0.428 0.439 0.434 0.433 0.434 0.427 0.430 0.430 0.403 0.409 0.429 0.413 0.440 0.451 0.428 0.440 0.439 0.454 0.452 0.448 0.418 0.408 0.413 0.413 0.414 0.413 0.407 0.411 0.438 0.434 0.428 0.433 0.426 0.427 0.430 0.428 0.443 0.438 0.443 0.442 0.411 0.405 0.408 0.408 0.418 0.416 0.421 0.419 0.431 0.449 0.435 0.439 0.408 0.406 0.404 0.406 0.407 0.410 0.410 0.409
% cholesterol absorption 29.90 27.59 30.62 31.38 43.48 43.04 46.68 39.18 41.48 34.11 47.36 47.73 49.77 46.58 45.55 49.82 50.06 47.37 48.02 46.36 50.43 49.16 46.72 50.69 50.34
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Appendix to TNO-report of 3M-02
G roup#
1
1 AVERAGE
SD 2 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3 3
AVERAGE SD 4 4 4 4 4 4 4 4 4 4
AVERAGE SD 5 5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6 6
AVERAGE SD 7 7 7 7 7 7 7 7 7 7
AVERAGE SD
M ouse# 1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
% cholestero l absorption 4 5 .8 9 4 3 .4 8 2 5 .7 8 3 2 .9 0 3 0 .6 7 4 3 .9 5 3 0 .3 8 4 4 .1 5 3 8 .7 3 3 3 .3 8 36.9 7.2 3 3 .0 3 3 1 .8 7 4 1 .1 0 2 9 .5 6 3 5 .2 7 3 2 .7 3 2 4 .9 2 3 8 .3 9 40.11 3 2 .3 0 33.9 5.0 4 2 .3 9 3 8 .9 3 3 0 .3 7 2 9 .7 4 3 3 .7 4 3 2 .5 0 3 4 .8 7 3 3 .1 9 4 7 .8 2 20.11 34.4 7.5 4 4 .6 2 3 5 .7 2 4 0 .6 3 3 9 .6 9 4 7 .9 2 4 7 .5 3 4 2 .4 3 4 1 .9 9 4 0 .9 0 4 0 .9 3 42.2 3.7 3 5 .6 9 4 0 .0 8 3 2 .2 6 3 7 .1 2 3 5 .0 2 2 9 .9 0 2 7 .5 9 3 0 .6 2 3 1 .3 8 4 3 .4 8 34.3 4.9 4 3 .0 4 4 6 .6 8 3 9 .1 8 4 1 .4 8 34.11 4 7 .3 6 4 7 .7 3 4 9 .7 7 4 6 .5 8 4 5 .5 5 44.1 4.7 4 9 .8 2 5 0 .0 6 4 7 .3 7 4 8 .0 2 4 6 .3 6 5 0 .4 3 4 9 .1 6 4 6 .7 2 5 0 .6 9 5 0 .3 4 48.9 1.6
Final version
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Appendix to TNO-report of 3M-02
Final version
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week 8/9
week 8/9
week 8/9
Intestinal cholesterol absorption, p values Mann-Whitney U-test
group 1 vs 2 | group 1 vs 3 group 1 vs 4
group 1 vs 5
group 1 vs 6
group 1 vs 7
n ni p n n p n n p n n p n n
p
nn
p
10 10 0.315 10 10 0.529 10 10 0.143 10 10 0.353 10 10 0.015 10 10 <0.001
group 2 vs 4 group 2 vs 5 group 2 vs 6 | group 2 vs 7
group 3 vs 4
group 3 vs 5
n n p n n p n_ n p n m p n n
p
nn
p
10 10 <0.001 10 1| 0.912 10 10 <0.001 10 101 <0.001 10 10 0.009 10 10 1.000
group 3 vs 7 | group 4 vs 5 group 4 vs 6 | group 4 vs 7
group 5 vs 6
group 5 vs 7
n n p nn
p n n p nn
p nn
p
nn
p
10 10 <0.001 10 101 0.002 10 10 0 . 3 1 ^ 10 101 <0.001 10 10 0.001 10 10 1<0.001
group 2 vs 3
nn p 10 10 0.796 group 3 vs 6 nn p 10 10 0.005 group 6 vs 7 nn p 10 101 0.003
p<0.05
Page A126 of A172
Appendix to TNO-report of 3M-02
Final version
Appendix G. Liver weight and perigonadal fat weight and lipid contents liver
G1. Liver weight and perigonadal fat weight
group
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6
AVERAGE SD
7 7 7 7 7 7 7 7 7 7 AVERAGE SD
M ouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
eartag LR
01 12 00 20 00 02 11 00 02 12
bodyw eight
(g) 3 4 .5 3 1 .9 3 0 .4 2 8 .9 3 2 .4 30.1 2 8 .4 3 2 .8
32 28
10 21 02 01 10 01 20 00 21 10
35.2 3 0 .3
34 3 4 .9 3 0 .4 3 1 .3 3 2 .9 29.2 2 8 .8 31.2
01 02 01 11 10 01 20 12 02 10
31 31 3 3 .6 2 9 .8 27.7 3 0 .9 31.2 2 6 .3 3 2 .3 2 9 .6
10 02 00 10 01 02 00 11 00 11
2 8 .3 2 8 .5 29.1 2 9 .8 31.1 2 6 .4 2 7 .6 30.1 2 7 .8 2 8 .3
10 01 11 12 12 20 01 11 00 12
3 1 .8 2 5 .9 2 9 .6 29.2 2 9 .6 30.2 3 1 .9 30.1 29.2 2 9 .6
01 10 12 10 02 00 01 02 22 21
28 31.2 2 5 .9 2 7 .5 2 6 .9 2 5 .4 2 6 .6 2 7 .8
30 2 6 .4
20 10 02 00 12 00 00 20 10 01
2 9 .9 30
28.7 3 0 .3 2 9 .4 28. 3 28.1
28 25 28
perig onadal fat w e ight (g) 1.06 0.72 0.68 0 .3 9 0.81 0.57 0 .6 3 0 .9 4 0 .8 9 0 .4 8 0.72 0.21 1.16 0 .5 3 0.92 0 .7 8 0 .5 5 0 .4 3 0 .5 0 0 .7 4 0.87 0.92 0 .7 4 0 .2 3 0.77 0 .7 4 0 .5 9 0.37 0.42 0 .6 4 0 .7 5 0 .4 4 1.05 0 .3 3 0.61 0 .2 3 0 .2 4 0 .2 6 0 .4 3 0.42 0 .4 5 0 .3 9 0 .2 3 0 .3 0 0 .3 4 0.32 0 .3 4 0 .0 8 0.82 0 .5 4 0 .6 5 0 .3 9 0.51 0.57 0 .4 4 0.52 0.86 0.47 0 .5 8 0 .1 6 0 .2 3 0 .3 4 0 .1 4 0 .1 9 0 .2 5 0 .3 0 0.41 0.37 0.31 0.21
0 .2 8 0 .0 9
0 .3 4 0 .3 6 0 .3 3 0 .3 8 0 .3 0 0 .3 0 0 .3 5 0.32 0 .2 9 0 .3 0 0 .3 3 0 .0 3
liver w e ight liver w e ight
(g) 2 .1 9
2 1.66
as % o f bw (% ) 6 .3 5 6.27 5 .4 6
1.41 1.68
4 .8 8 5 .1 9
1.7 5 .6 5
1.61
5.67
1.77 1.68
5 .4 0 5 .2 5
1.49
5.32
1.72 0.23
5 .5 4 0 .4 6
2.77
7.87
1.17 1.88
3 .8 6 5 .5 3
2 .5 3 1.68
7 .2 5 5 .5 3
1.23
3 .9 3
1.35
4 .1 0
1.84 1.53
6 .3 0 5.31
1.98
6 .3 5
1.80
5 .6 0
0.53
1.38
2 6.45
2 .2 3
7 .1 9
2.27 1.68
6 .7 6 5 .6 4
1.56
5 .6 3
2 .0 6
6.67
1.85 1.51
5 .9 3 5 .7 4
2.42
7 .4 9
2.14
7 .2 3
1.97
6.47
0.31
0.71
3 .4 6
12.23
3 .6 8 3 .6 9 3 .9 3
12.91 12.68 13.19
4 .2 3
13.60
1.94
7 .3 5
3.62
13.12
3.9 12.96
4 .0 3 3.39
14.50 11.98
3 .5 9
12.45
0.63
1.92
1.9 5.97
1.71 2 .4 8
6 .6 0 8 .3 8
1.69
5 .7 9
2 .2 5
7 .6 0
2 .4 6
8 .1 5
1.47 1.88 1.82
4.61 6 .2 5 6 .2 3
2.14
7 .2 3
1.98 0.34
6.68 1.16
3.9 13.93
4.87 4.1
15.61 15.83
4.17
15.16
4 .1 3
15.35
3 .4 8
13.70
3 .1 3
11.77
4.11 4 .7 4
14.78 15.80
4.33
16.40
4 .1 0 0.52
14.83 1.37
4 .2 9 4 .3 3
14.35 14.43
3 .8 8
13.52
4 .2 8
14.13
4 .1 5
14.12
3 .8 5
13.60
3.91 3 .8 3
13.91 13.68
3 .3 3
13.32
3.92
14.00
3 .9 8
13.91
0.30
0.37
04-12-2006
Appendix to TNO-report of 3M-02
Final version
04-12-2006
tissue
perigonadal fat liver
liver as % of bodyweight
tissue
perigonadal fat liver
liver as % of bodyweight
tissue
perigonadal fat liver
liver as % of bodyweight
Liverweight; perigonadal fat weight; liverweight as % of bodyweight, p values Mann-Whitney U-test
group 1 vs 2 group 1 vs 3 group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7 group 2 vs 3
nn p nn p nn E nn p nn p nn p nn p 10 10 0.912 10 10 0.315 10 10 <0.001 10 10 0.123 10 10 <0.001 10 10 <0.001 10 10 0.190
10 10 0.971 10 10 0.075 10 10 <0.001 10 10 0.043 10 10 <0.001 10 10 <0.001 10 10 0.247
10 10 0.796 10 10 0.003 10 10 <0.001 10 10 0.015 10 10 <0.001 10 10 <0.001 10 10 0.089
group 2 vs 4 group 2 vs 5 group 2 vs 6 group 2 vs 7 group 3 vs 4 group 3 vs 5 group 3 vs 6
nn p nn p nn p nn p nn p nn p nn p 10 10 <0.001 10 10 0.123 10 10 <0.001 10 10 <0.001 10 10 0.003 10 10 0.971 10 10 <0.001
10 10 <0.001 10 10 0.315 10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 0.912 10 10 <0.001
10 10 <0.001 10 10 0.105 10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 0.529 10 10 <0.001
group 3 vs 7 group 4 vs 5 group 4 vs 6 | group 4 vs 7 group 5 vs 6 group 5 vs 7 group 6 vs 7
nn p nn p nn p nn p nn p nn p nn p 10 10 <0.001 10 10 <0.001 10 10 0.105 10 10 0.796 10 10 <0.001 10 10 <0.001 10 10 0.190
10 10 <0.001 10 10 <0.001 10 10 0.043 10 10 0.105 10 10 <0.001 10 10 <0.001 10 10 0.481
10 10 <0.001 10 10 <0.001 10 10 0.003 10 10 0.003 10 10 <0.001 10 10 <0.001 10 10 0.029
p<0.05
Page A128 of A172
Appendix to TNO-report of 3M-02
Final version
G2. Lipid contents liver Liverhomogenates; protein concentration
mouse
i 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
measured prot conc (mg/ml)
well 1
well 2
0.551
0.56
0.292
0.301
0.701
0.686
1.058
1.098
0.384
0.389
0.468
0.46
1.208
1.279
0.468
0.46
0.479
0.468
0.596
0.587
1.011
0.949
0.707
0.692
1.406
1.44
1.185
1.185
0.35
0.341
0.59
0.579
0.768
0.772
0.622
0.607
0.484
0.494
1.022
1.091
0.942
0.945
0.75
0.734
0.734
0.71
0.508
0.516
0.747
0.722
0.407
0.407
1.054
1.047
0.778
0.765
0.489
0.468
0.185
0.187
0.263
0.274
0.822
0.829
0.546
0.573
1.251
1.231
1.627
1.608
0.524
0.529
0.579
0.579
0.533
0.541
0.296
0.321
0.758
0.749
0.707
0.71
0.453
0.455
0.468
0.478
0.433
0.425
0.438
0.438
1.099
1.151
0.13
0.13
0.93
0.934
1.953
2.04
0.428
0.415
0.36
0.351
0.463
0.458
1.124
1.147
0.481
0.465
0.372
0.363
0.779
0.801
0.465
0.478
0.568
0.554
0.776
0.755
0.173
0.169
0.62 0.612
2.018
2.035
0.692
0.674
0.321
0.314
0.341
0.341
0.239
0.235
0.355
0.348
0.175
0.177
0.743
0.728
1.444
1.462
dilution Protein conc (mg/ml)
20 11.11 20 5.93 20 13.87 20 21.56 20 7.73 20 9.28 20 24.87 20 9.28 20 9.47 20 11.83 20 19.60 20 13.99 20 28.46 20 23.70 20 6.91 20 11.69 20 15.40 20 12.29 20 9.78 20 21.13 20 18.87 20 14.84 20 14.44 20 10.24 20 14.69 20 8.14 20 21.01 20 15.43 20 9.57 20 3.72 20 5.37 20 16.51 20 11.19 20 24.82 20 32.35 20 10.53 20 11.58 20 10.74 20 6.17 20 15.07 20 14.17 20 9.08 20 9.46 20 8.58 20 8.76 20 22.50 20 2.60 20 18.64 20 39.93 20 8.43 20 7.11 20 9.21 20 22.71 20 9.46 20 7.35 20 15.80 20 9.43 20 11.22 20 15.31 20 3.42 20 12.32 20 40.53 20 13.66 20 6.35 20 6.82 20 4.74 20 7.03 20 3.52 20 14.71 20 29.06
04-12-2006
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Appendix to TNO-report of 3M-02
Final version
04-12-2006
HPTLC measurements, liver lipids:
mouse# 1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33
Peak# 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4
Name
Width [mm]
Free cholesterol
4.57
Triglycerides
8.13
Internal standard Cholesterolester
3.94 4.19
Free cholesterol
4.57
Triglycerides Internal standard
6.22 4.06
Cholesterolester
5.08
Free cholesterol
4.57
Triglycerides
7.87
Internal standard
3.94
Cholesterolester
4.06
Free cholesterol Triglycerides
3.81 5.46
Internal standard Cholesterolester
4.19 4.32
Free cholesterol
4.06
Triglycerides
6.99
Internal standard
4.19
Cholesterolester
4.83
Free cholesterol
4.57
Triglycerides
7.62
Internal standard Cholesterolester
4.32 4.7
Free cholesterol
4.19
Triglycerides
7.75
Internal standard Cholesterolester
4.06 4.32
Free cholesterol
4.57
Triglycerides Internal standard
6.73 4.06
Cholesterolester
4.45
Free cholesterol
4.32
Triglycerides
7.11
Internal standard
4.06
Cholesterolester
4.32
Free cholesterol Triglycerides
4.06 7.49
Internal standard Cholesterolester
3.17 3.81
Free cholesterol
4.45
Triglycerides
5.71
Internal standard
3.81
Cholesterolester
4.19
Free cholesterol
4.45
Triglycerides
8.64
Internal standard Cholesterolester
3.17 4.57
Free cholesterol
4.83
Triglycerides
8
Internal standard Cholesterolester
4.32 4.57
Free cholesterol
4.45
Triglycerides Internal standard
6.6 3.94
Cholesterolester
4.83
Free cholesterol
4.95
Triglycerides
4.83
Internal standard
4.06
Cholesterolester
3.94
Free cholesterol Triglycerides
4.45 6.6
Internal standard Cholesterolester
3.68 4.7
Free cholesterol
4.06
Triglycerides
6.86
Internal standard Cholesterolester
3.81 5.08
Free cholesterol
4.57
Trig lycerides Internal standard
6.22 4.19
Cholesterolester
4.57
Pos [mm] 6.73 21.84 27.3 35.31
6.22 19.94 26.42 34.8 5.97 19.68 26.16 34.42 6.35 19.43 26.42 34.8
6.35 20.32 26.42 34.67 6.48 20.07 26.29 34.42 6.48 20.07 26.16 34.16
6.99 20.32 26.54 34.54 6.86 20.83 26.16 33.66 6.86 21.97 26.29 33.66
7.62 23.24 27.56 34.16 6.48 22.86 27.56 35.31 6.48 20.83 26.54 34.54
6.22 19.56 25.65 34.16 5.97 18.41 25.4 33.78 6.22 20.07 26.16 34.29
6.35 21.34 26.67 34.54 6.86 22.23 27.3 34.92
od-Bkg 62604 287988 22996 105220
52524 197880 24262 125607 43766 251729 26977 68880 34466 123478 27048 67378
52422 260322 24841 141768 50585 265253 24749 127056 48224 248371 28494 130184
44510 192428 25345 79085 54357 258846 23946 89672 57023 299353 22427 84768
39787 115041 21069 81470 51326 318787 21609 111253 41327 242241 25212 94035
46397 221527 27238 133113 35829 104710 25858 42916 44899 206251 22142 78487
51469 216844 25800 74388 46317 162768 26102 89651
|jg lipid/ 200 pg protein
5.44
50.09
2.00
9.15
4.33
32.62
2.00
10.35
3.24
37.32
2.00
5.11
2.55
18.26
2.00
4.98
4.22
41.92
2.00
11.41
4.09
42.87
2.00
10.27
3.38
34.87
2.00
9.14
3.51
30.37
2.00
6.24
4.54
43.24
2.00
7.49
5.09
53.39
2.00
7.56
3.78
21.84
2.00
7.73
4.75
59.01
2.00
10.30
3.28
38.43
2.00
7.46
3.41
32.53
2.00
9.77
2.77
16.20
2.00
3.32
4.06
37.26
2.00
7.09
3.99
33.62
2.00
5.77
3.55
24.94 2.00 6.87
Paae A130 of A172
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Final version
04-12-2006
mouse# 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5
Peak#
1 2 3 4 1 2 3 4
1 2 3 4
1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4
1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4
Name
Width [mm]
Free cholesterol
5.08
Triglycerides
5.59
Internal standard
4.06
Cholesterolester
4.06
Free cholesterol
4.19
Triglycerides
6.86
Internal standard Cholesterolester
3.94 3.94
Free cholesterol
4.45
Triglycerides
7.49
Internal standard Cholesterolester
3.3 4.45
Free cholesterol
4.32
Triglycerides Internal standard
5.33 4.32
Cholesterolester
3.56
Free cholesterol
4.19
Triglycerides
8.51
Internal standard Cholesterolester
3.56 5.33
Free cholesterol Triglycerides
3.94 5.97
Internal standard Cholesterolester
4.45 4.95
Free cholesterol
4.19
Triglycerides
6.48
Internal standard
4.06
Cholesterolester
5.33
Free cholesterol
4.45
Triglycerides
6.48
Internal standard Cholesterolester
3.94 5.08
Free cholesterol
4.06
Triglycerides
6.6
Internal standard Cholesterolester
3.94 5.59
Free cholesterol
4.06
Triglycerides Internal standard
6.99 4.32
Cholesterolester
6.1
Free cholesterol
4.7
Triglycerides
5.97
Internal standard Cholesterolester
3.56 4.06
Free cholesterol Triglycerides
3.94 5.97
Internal standard
4.32
Cholesterolester
5.59
Free cholesterol
3.81
Triglycerides
5.46
Internal standard
3.94
Cholesterolester
4.45
Free cholesterol
4.32
Triglycerides
6.73
Internal standard Cholesterolester
4.06 4.95
Free cholesterol
4.7
Triglycerides
7.24
Internal standard Cholesterolester
3.94 4.95
Free cholesterol
3.68
Triglycerides Internal standard
6.6 3.56
Cholesterolester
5.59
Free cholesterol
3.81
Triglycerides
6.6
Internal standard Cholesterolester
3.81 5.84
Free cholesterol Triglycerides
3.56 5.71
Internal standard Choleste rolester
4.19 5.59
Pos [mm]
7.37 22.61 27.69 35.31 7.24 22.99 27.81 35.31
6.73 23.11 27.43 35.05
7.37 23.37 28.45 36.07 5.46 21.21 26.16 34.04
5.46 18.8 25.27 33.66 4.95 17.02 24 33.02 4.57 16 23.24 32.64
4.7 15.75 23.11 32.64 5.21
16 23.24 32.64 6.22 19.68 26.29 34.29
4.45 15.37 22.86 32.13
5.33 17.14 24.13 33.02 5.59 19.05 24.77 33.15
5.46 20.57 25.53 33.4 3.94 13.97 21.21 30.35 3.94 13.84 20.96 30.35
4.19 13.08 20.7 30.23
od-Bkg
39765 132487 27644 37392 49635 246286 23327 123642
60183 294481 22239 141459
38024 104943 29874 26558 35962 307860 24049 107934
40358 115325 25364 52857 42991 172245 24996 73646 56968 192630 26017 123337
56224 215795 27127 153105 55767 238689 31249 162067 53651 199383 25618 75588
38266 151763 32037 54371
49570 149995 26617 46177 61124 219127 28657 145012
56257 295337 28462 97524 48925 249297 28191 157664 65477 276227 27578 178277
49411 189703 29682 95600
|jg lipid/ 200 |jg protein
2.88 19.17 2.00 2.71 4.26 42.23 2.00 10.60
5.41 52.97 2.00 12.72
2.55 14.05 2.00 1.78 2.99 51.20 2.00 8.98
3.18 18.19 2.00 4.17 3.44 27.56 2.00 5.89 4.38 29.62 2.00 9.48
4.15 31.82 2.00 11.29 3.57 30.55 2.00 10.37 4.19 31.13 2.00 5.90
2.39 18.95 2.00 3.39
3.72 22.54 2.00 3.47 4.27 30.59 2.00 10.12
3.95 41.51 2.00 6.85 3.47 35.37 2.00 11.19 4.75 40.07 2.00 12.93
3.33 25.57 2.00 6.44
Page A131 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mouse# 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48
Peak# 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4
1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4
Name
Width [mm]
Free cholesterol Triglycerides
3.3 5.71
Internal standard Cholesterolester
4.19 5.33
Free cholesterol
3.43
Triglycerides
7.49
Internal standard
3.81
Cholesterolester
5.59
Free cholesterol
3.94
Triglycerides
6.35
Internal standard Cholesterolester
3.81 5.46
Free cholesterol
3.56
Triglycerides Internal standard Cholesterolester
5.71 3.81 4.95
Free cholesterol
3.94
Triglycerides
6.6
Internal standard Cholesterolester
3.68 5.33
Free cholesterol
4.19
Triglycerides
7.37
Internal standard
3.68
Cholesterolester
5.46
Free cholesterol
4.45
Triglycerides
5.97
Internal standard Cholesterolester
4.19 4.7
Free cholesterol
4.7
Triglycerides
6.35
Internal standard Cholesterolester
4.19 3.68
Free cholesterol
4.83
Triglycerides Internal standard
6.99 4.7
Cholesterolester
4.57
Free cholesterol
4.32
Triglycerides
6.22
Internal standard Cholesterolester
4.06 4.45
Free cholesterol Triglycerides
3.94 6.6
Internal standard
3.81
Cholesterolester
4.06
Free cholesterol
4.19
Triglycerides
5.71
Internal standard
3.68
Cholesterolester
4.06
Free cholesterol
4.7
Triglycerides
7.49
Internal standard Cholesterolester
3.43 5.08
Free cholesterol
3.81
Triglycerides
5.59
Internal standard Cholesterolester
3.81 4.06
Free cholesterol
3.94
Triglycerides Internal standard
5.84 3.94
Cholesterolester
4.45
Free cholesterol
4.32
Triglycerides
5.71
Internal standard Cholesterolester
3.81 4.45
Free cholesterol Triglycerides
4.06 5.59
Internal standard
3.81
Cholesterolester
4.45
Free cholesterol
4.32
Triglycerides
6.6
Internal standard
3.17
Cho leste rolester
4.7
Pos [mm] 3.94 12.57 20.45 30.1
3.94 13.84 21.08 30.73 4.32 14.48 22.1 31.5 4.19 14.73 22.48 31.62
4.57 16.13 23.37 32.26 5.33 18.16 24.51 33.02 5.33 18.92 25.27 33.27
5.59 20.32 25.91 33.27 5.84 21.21 27.69 35.69 5.71 20.45 26.92 34.92
5.97 20.07 26.42 34.54
5.97 19.3 25.65 33.78 6.48 20.45 25.65 33.66
6.99 20.45 25.91 33.66 7.11 21.21 25.91 33.53 6.48 20.83 25.53 32.89
7.37 22.23 26.8 33.91 7.75 23.88 27.43 34.16
od-Bkg 51117 186121 31056 108021
51578 314298 30069 134094 49352 209014 27111 117556 43993 173724 24940 61245
53573 223574 24814 161398 55464 270083 24336 153330 51342 176085 23750 76943
47717 148024 25114 60069 45004 193690 27970 60245 43432 150439 25551 98765
42568 194874 23418 72154
40720 177809 27101 69768 52458 292884 29963 160643
50307 162127 27192 70970 56774 225869 27244 91446 49756 171023 26319 87109
41083 144165 27516 61184 55902 285090 26432 119215
|jg lipid/ 200 |jg protein 3.29 23.97 2.00 6.96
3.43 41.81 2.00 8.92 3.64 30.84 2.00 8.67 3.53 27.86 2.00 4.91
4.32 36.04 2.00 13.01 4.56 44.39 2.00 12.60 4.32 29.66 2.00 6.48
3.80 23.58 2.00 4.78 3.22 27.70 2.00 4.31 3.40 23.55 2.00 7.73
3.64 33.29 2.00 6.16
3.01 26.24 2.00 5.15 3.50 39.10 2.00 10.72
3.70 23.85 2.00 5.22 4.17 33.16 2.00 6.71 3.78 25.99 2.00 6.62
2.99 20.96 2.00 4.45 4.23 43.14 2.00 9.02
Page A132 of A172
Appendix to TNO-report of 3M-02
Final version
mouse# 58 68 9 19 29 39 49 59 69 10 20 30 40 50 60 70
Peak#
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
1 2 3 4
Name
Width [mm] Pos [mm]
Free cholesterol 3.81
7.11
Triglycerides 6.73 23.24
Internal standard 3.43
27.43
Cholesterolester 4.57
34.16
Free cholesterol 3.56
4.57
Triglycerides 8.89 14.61
Internal standard 4.06
22.1
Cholesterolester 5.71
32.13
Free cholesterol 3.17
4.57
Triglycerides 5.59 12.19
Internal standard 3.81
20.83
Cholesterolester 4.95
31.24
Free cholesterol
3.3
4.32
Triglycerides 4.7 11.68
Internal standard 3.94
20.57
Cholesterolester 4.95
31.12
Free cholesterol
3.3
3.81
Triglycerides 6.99 12.57
Internal standard 3.68
20.57
Cholesterolester 5.33
31.12
Free cholesterol 3.56
3.81
Triglycerides 5.08 11.68
Internal standard 4.19
20.7
Cholesterolester 5.33
31.24
Free cholesterol 3.94
4.06
Triglycerides 4.83 11.81
Internal standard 4.32
21.34
Cholesterolester 4.95
32.13
Free cholesterol 3.94
3.81
Triglycerides 5.97 12.57
Internal standard 4.06
21.84
Cholesterolester 5.71
32.38
Free cholesterol 3.17
4.7
Triglycerides 8.13 14.86
Internal standard 4.32
22.99
Cholesterolester 5.46
33.4
Free cholesterol 3.43
4.57
Triglycerides
5.21
13.59
Internal standard 3.68
22.99
Cholesterolester 4.83
33.27
Free cholesterol 3.43
4.83
Triglycerides 6.22 14.99
Internal standard 3.68
23.75
Cholesterolester 4.7
33.91
Free cholesterol 3.17
5.08
Triglycerides 5.08 15.49
Internal standard 3.68
24.38
Cholesterolester 4.32
34.16
Free cholesterol 4.06
8.76
Triglycerides 5.46 27.43
Internal standard 2.92
30.1
Cholesterolester 4.83
35.18
Free cholesterol 3.56
8.89
Triglycerides 4.83 25.78
Internal standard 3.43
29.34
Cholesterolester 3.94
35.18
Free cholesterol 4.19
8
Triglycerides 6.35 24.77
Internal standard 2.79
28.57
Cholesterolester 4.83
35.05
Free cholesterol
4.7
7.49
Triglycerides 7.49 23.11
Internal standard 3.17
27.81
Cholesterolester 4.45
34.8
od-Bkg
50057 254148 23859 136982
59495 368098 29699 172395
47364 186870 29695 97139
44604 114977 29508 85494
55495 290613 29976 133502
54475 146150 30815 126727
63475 140542 33224 84388
64340 238585 30644 139703
56623 368599 30442 178078
37007 124055 26723 78711
47883 216520 26901 83366
34039 136553 27780 92480
54261 222500 24418 136191
36509 87808 28028 35295
58652 238639 21843 134627
58311 269150 31958 124736
|jg lipid/ 200 gg protein
4.20 42.61 2.00 11.48
4.01 49.58 2.00 11.61
3.19 25.17 2.00 6.54
3.02 15.59 2.00 5.79
3.70 38.78 2.00 8.91
3.54 18.97 2.00 8.23
3.82 16.92 2.00 5.08
4.20 31.14 2.00 9.12
3.72 48.43 2.00 11.70
2.77 18.57 2.00 5.89
3.56 32.20 2.00 6.20
2.45 19.66 2.00 6.66
4.44 36.45 2.00 11.15
2.61 12.53 2.00 2.52
5.37 43.70 2.00 12.33
3.65 33.69 2.00 7.81
04-12-2006
Page A133 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
G roup#
AVERAGE SD 2 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3 3
AVERAGE SD
i
AVERAGE SD 5 5 5 5 5 5 5 5 5 5
AVERAGE SD 6
6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
M ouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
Liver lipids (pg/200 pg protein)
Free cholesterol T riglycerides C h oleste ro le ste r
5.44 3.51 2.77
5 0 .0 9 30.37 16.20
9.15 6.24 3.32
2.55 3.33
14.05 25.57
1.78 6.44
4.19 4.32
3 1 .1 3 2 9 .6 6
5.90 6.48
3.70
2 3 .8 5
5.22
3.19 2.77
25.17 18.57
6.54 5.89
2.99 2.39 4.33 4.54 3.29 3.80 4.06 4.17 3.02 3.56
5 1 .2 0 18.95 32.62 4 3 .2 4 23.97 2 3 .5 8 3 7 .2 6 3 3 .1 6 15.59 3 2 .2 0
8.98 3.39 10.35 7.49 6.96 4.78 7.09 6.71 5.79 6.20
3.24 5.09 3.99 3.18 3.72 3.43 3.22 3.78 3.70 2.61
37.32 5 3 .3 9 33.62 18.19 2 2 .5 4 41.81 2 7 .7 0 2 5 .9 9 3 8 .7 8 12.53
5.11 7.56 5.77 4.17 3.47 8.92 4.31 6.62 8.91 2.52
2.55 3.78 3.55 3.44 4.27 3.64 3.40 2.99 3.54 2.45
18.26 2 1 .8 4 2 4 .9 4 2 7 .5 6 3 0 .5 9 3 0 .8 4 2 3 .5 5 2 0 .9 6 18.97 19.66
4.98 7.73 6.87
5.89 10.12 8.67 7.73
4.45 8.23 6.66
2.88 4.38 3.95 3.53 4.22 4.75 3.64 4.23 3.82 5.37
19.17 29.62 41.51 2 7 .8 6 41.92 59.01 3 3 .2 9 4 3 .1 4 16.92 4 3 .7 0
2.71 9.48 6.85 4.91 11.41 10.30 6.16 9.02 5.08 12.33
4.09 3.28 4.26 4.15 3.47 4.32 3.01 4.20 4.20 4.44
42.87 3 8 .4 3 4 2 .2 3 31.82 35.37 3 6 .0 4 2 6 .2 4 42.61 3 1 .1 4 3 6 .4 5
10.27 7.46 10.60 11.29 11.19 13.01 5.15 11.48 9.12 11.15
3.38 3.41 5.41 3.57 4.75 4.56 3.50 4.01 3.72 3.65
34.87 3 2 .5 3 52.97 3 0 .5 5 40.07 4 4 .3 9 3 9 .1 0 4 9 .5 8 4 8 .4 3 3 3 .6 9
9.14 9.77 12.72 10.37 12.93 12.60 10.72 11.61 11.70 7.81
Liver lipids (pg/m g protein)
|
Free cholestero l T riglycerides C h ole ste ro le ste r
27.22
2 5 0 .5
45.76
17.56
1 5 1 .8
31.20
13.86
81.0
16.60
12.73
70.3
8.89
16.65
1 2 7 .8
32.21
2 0 .9 4
1 5 5 .7
29.51
21.62
1 4 8 .3
32.40
18.50
1 1 9 .2
26.10
15.95
1 2 5 .9
32.71
13.85
92.8
29.45
17.89
1 3 2 .3
28.48
4.41
51.0
9.92
14.95
2 5 6 .0
44.88
11.94
94.7
16.97
2 1 .6 5
163.1
51.77
2 2 .7 0
2 1 6 .2
37.45
16.46
1 1 9 .9
34.78
19.00
1 1 7 .9
23.92
2 0 .2 8
1 8 6 .3
35.45
2 0 .8 4
1 6 5 .8
33.57
15.12
77.9
28.97
17.80
1 6 1 .0
30.99
18.07
1 5 5 .9
33.87
3.44
55.1
9.86
16.22
1 8 6 .6
25.53
2 5 .4 3
2 6 7 .0
37.80
19.95
168.1
28.83
15.91
90.9
20.84
18.62
1 1 2 .7
17.35
17.15 16.09
2 0 9 .0 1 3 8 .5
44.59 21.54
18.90
1 3 0 .0
33.10
18.51 13.03
1 9 3 .9 62.7
44.54 12.59
17.98
1 5 5 .9
28.67
3.28
61.0
11.14
12.74
91.3
24.91
18.88 17.74
1 0 9 .2 1 2 4 .7
38.67 34.35
17.20
1 3 7 .8
29.46
2 1 .3 3
1 5 2 .9
50.60
18.20
1 5 4 .2
43.36
17.00
1 1 7 .8
38.65
14.93
1 0 4 .8
22.24
17.68
94.9
41.13
12.25
98.3
33.29
16.80
1 1 8 .6
35.67
2.78
23.2
8.64
14.38
95.9
13.53
2 1 .9 0
148.1
47.41
19.77
2 0 7 .5
34.26
17.64 21.10
1 3 9 .3 2 0 9 .6
24.56 57.07
2 3 .7 5
295.1
51.49
18.18
1 6 6 .4
30.81
2 1 .1 5
2 1 5 .7
45.10
19.10
84.6
25.40
2 6 .8 5
2 1 8 .5
61.64
2 0 .3 8
178.1
39.13
3.45
63.8
15.76
2 0 .4 4
2 1 4 .4
51.34
16.39 2 1 .2 8
1 9 2 .2 211.2
37.30 53.00
2 0 .7 3
159.1
56.44
17.35
1 7 6 .9
55.93
2 1 .5 9
1 8 0 .2
65.04
15.03
1 3 1 .2
25.74
2 0 .9 8 21.00 22.22
2 1 3 .0 1 5 5 .7 1 8 2 .2
57.41 45.59 55.77
19.70
1 8 1 .6
50.36
2.49
27.5
11.40
16.92
1 7 4 .3
45.69
17.03
1 6 2 .7
48.87
2 7 .0 6 17.85
2 6 4 .8 1 5 2 .8
63.61 51.86
2 3 .7 4 2 2 .7 9
2 0 0 .3 222.0
64.65 63.01
17.51
1 9 5 .5
53.61
2 0 .0 3
2 4 7 .9
58.05
18.60
2 4 2 .2
58.50
18.25
1 6 8 .4
39.03
19.98
203.1
54.69
3.43
39.4
8.45
Page A134 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
lipid
free cholesterol triglyceride
cholesterolester
lipid
free cholesterol triqlvceride
cholesterolester
lipid
free cholesterol triqlvceride
cholesterolester
group 1 vs 2 nn p 10 10 0.739 10 10 0.247 10 10 0.218 group 2 vs 4
nn p 10 10 0.353 10 10 0.089 10 10 0.684
group 3 vs 7 nn p 10 10 0.247 10 10 0.075 10 10 <0.001
group 1 vs 3
nn p 10 10 0.796 10 10 0.353
10 10 0.912
group 2 vs 5
nn p 10 10 0.218 10 10 0.436
10 10 0.529
group 4 vs 5
nn p 10 10 0.023 10 10 0.035
10 10 0.579
lipid contents liver, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5 group 1 vs 6
nn
p nn p nn
p
10 10 0.853 10 10 0.105 10
0.218
10 10 0.631 10 10 0.105 10
0.004
10 10 0.105 10 10 0.190 10
0.002
group 2 vs 6 group 2 vs 7 group 3 vs 4
nn E nn p nn
p
10 10 0.315 10 10 0.353 10 10 0.529
10 10 0.280 10 10 0.052 10 10 0.143
10 10 0.004 10 10 <0.001 10 10 0.143
group 4 vs 6 | group 4 vs 7 group 5 vs 6
nn p
nn p nn
p
10 10 0.052 10 10 0.075 10 10 0.739
10 10 <0.001 10 10 <0.001 10 10 0.971
10 10 0.005 10 10 <0.001 10 10 0.105
group 1 vs 7
nn p 10 10 0.190 10 10 0.002
10 10 <0.001
group 3 vs 5
nn p 10 10 0.089 10 10 0.353
10 10 0.105
group 5 vs 7
nn p 10 10 0.579 10 10 0.315
10 10 0.015
group 2 vs 3 nn p 10 10 0.739 10 10 0.912 10 10 0.280 group 3 vs 6 nn p 10 10 0.089 10 10 0.247 10 10 0.001 group 6 vs 7 nn p 10 10 0.971 10 10 0.315 10 10 0.393
p<0.05
Page A135 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
H. HMG-CoA reductase and cholesterol-7a-hydroxylase activity in liver microsomes and sterol27-hydroxylase activity in liver mitochondria
H1. HMG-CoA reductase and cholesterol-7a-hydroxylase activity in liver microsomes
Protein concentration isolated microsomes
M ic ro s o m e s fo r H M G C o A re d u c ta s e d e te rm in a tio n
m ouse
1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
m easured p ro t co n c (m g/m l)
w ell 1 0 .8 2 8
w ell 2 0.866
0 .7 2 5
0 .7 3 9
1 .0 5 2
1.102
1.42
1 .5 2 5
0 .9 2 7
0 .9 7 7
0 .6 5 9 0 .6 5 4
0 .6 5 4 0.688
0 .7 6 3
0.801
1 .0 1 9
1 .0 7 3
0 .7 8 8
0 .8 1 8
1 .6 6 7
1 .8 0 3
1.031
1 .0 5 6
0 .6 4
0 .6 4 7
0.691
0 .7 0 9
0 .7 8 9
0 .8 2 8
0 .6 6 9
0.688
0 .7 1 8
0 .7 3 9
0 .8 7 6
0.888
0 .6 6 4 1.86
0 .6 7 7 1 .9 8 3
1 .7 3 3
1.851
0 .5 8 3
0 .6 0 4
0 .7 5 2
0 .7 7 3
0 .7 1 8
0 .7 2 9
1 .1 2 4
1.161
0 .7 3 4
0 .7 5 2
1 .5 5 5
1 .6 9 6
1 .7 5 6
1.961
0.7 0.723
0 .5 1 9
0.521
0 .8 2 2
0 .8 4 6
1 .2 2 3
1 .3 0 8
1 .1 3 6
1 .1 7 6
1 .6 4 9
1.791
0.651
0 .6 4 3
0 .6 2 2
0 .6 2 8
0 .9 8
1.041
0 .9 8
0.991
0 .7 6 3
0 .7 9 6
0.881
0.91
1 .1 4 9
1 .2 1 6
1.2 1.281
0.66
0 .7 3 4
0 .5 9 7
0 .6 4
1 .1 2 7
1 .1 9 8
1 .2 5 4
1 .3 1 5
0 .6 7
0 .6 9 9
1 .6 9 7
1 .7 1 2
1.641
1 .8 2 3
0 .6 3 7
0.668
0 .7 9 4
0 .8 2 6
0 .8 3 2
0 .8 5 9
1.63
1 .5 5 3
0 .7 9 6
0 .8 1 7
1 .7 4 7
1 .8 9 9
0 .6 3 6
0 .6 3 2
1 .0 9 7
1 .1 4 9
0 .7 3 6
0 .7 6 6
0 .9 2
0 .9 0 6
1 .5 0 3
1.47
0 .9 8
1 .0 3 4
0.621
0 .6 1 2
1 .1 5 4
1 .2 1 9
0 .7 9 8
0 .8 2
0 .8 0 7
0 .7 9 4
1.17
1 .2 0 3
1 .6 8 5
1 .6 1 2
0 .6 2 9
0 .6 5 7
1 .0 9 2
1 .0 4 8
1 .5 2 9
1 .5 9 8
d ilu tio n
20 20 20 20 20
60
60 20 20 20 20 20 60
60 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 60 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
60 20 20 20 20 20
60 20 20 20 20 20 20 20 20
P rotein conc (m g/m l) 1 6 .9 4 1 4 .6 4 2 1 .5 4 2 9 .4 5 1 9 .0 4 3 9 .3 9 4 0 .2 6 1 5 .6 4 2 0 .9 2 1 6 .0 6 3 4 .7 0 2 0 .8 7 38.61 4 2 .0 0 1 6 .1 7 1 3 .5 7 1 4 .5 7 1 7 .6 4 13.41 3 8 .4 3 3 5 .8 4 1 1 .8 7 1 5 .2 5 1 4 .4 7 2 2 .8 5 1 4 .8 6 32.51 3 7 .1 7 1 4 .2 3 1 0 .4 0 1 6 .6 8 25.31 2 3 .1 2 3 4 .4 0 3 8 .8 2 1 2 .5 0 20.21 19.71 1 5 .5 9 17.91 2 3 .6 5 24.81 1 3 .9 4 1 2 .3 7 2 3 .2 5 2 5 .6 9 1 3 .6 9 3 4 .0 9 3 4 .6 4 1 3 .0 5 1 6 .2 0 16.91 3 1 .8 3 1 6 .1 3 3 6 .4 6 3 8 .0 4 2 2 .4 6 1 5 .0 2 1 8 .2 6 2 9 .7 3 2 0 .1 4 3 6 .9 9 2 3 .7 3 1 6 .1 8 16.01 2 3 .7 3 3 2 .9 7 12.86 2 1 .4 0 3 1 .2 7
M ic ro s o m e s fo r 7 -a lp h a -h y d ro x y la s e d e te rm in a tio n
m ouse
1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
Code
1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 1.10 1.11 1.12 1.13 1.14 1.15 1.16 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 2.10 2.11 2.12 2 .1 3 2 .1 4 2 .1 5 2 .1 6 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 3 .1 0 3.11 3 .1 2 3 .1 3 3 .1 4 3 .1 5 3 .1 6 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4 .1 0 4.11 4 .1 2 4 .1 3 4 .1 4 4 .1 5 4 .1 6 5.1 5.2 5.3 5.4 5.5 5.6
m easured p ro t co n c (m g/m l)
w ell 1
w ell 2
0 .9 5 2
0 .9 9 6
0 .6 8 3
0 .7 0 3
1 .0 9 7
1 .0 4 8
1 .5 4 3
1 .4 3 8
1.222
1 .2 1 8
0 .6 5 2
0 .6 4 4
0 .6 1 2
0 .6 9 4
1 .1 0 3
1 .1 1 3
1 .3 6 6
1 .3 2 9
0 .9 9 9
0 .9 6 3
1 .5 2 4
1 .4 4 7
0.921
0 .9 0 9
0 .5 6 9
0 .5 7 5
0 .5 7 7
0.571
0 .7 0 8
0 .6 7 9
0 .5 5 3
0 .5 4 5
0 .9 1 8
0 .8 7 4
1.15
1.14
0.69
0 .6 9 4
0.5 0.436
0 .5 4 3
0 .4 7 9
0 .5 9 9
0 .5 6 9
0 .8 8 7
0 .8 3 7
0 .7 2 6
0 .7 2 4
0 .8 8 2
0 .8 9 3
0 .7 7 2
0 .8 2 9
0.541
0 .5 1 5
0.61
0 .5 7 9
0 .6 0 4
0 .6 2 4
0 .4 8 8
0 .4 9 8
0 .6 7 4
0 .6 7
1 .2 6 3
1 .3 2 9
1 .0 2 3
1 .0 5 5
0 .5 0 7
0 .4 7 2
0 .5 0 5
0 .4 9 2
0 .5 9 5
0.581
0 .8 0 8
0 .7 9
0 .9 9 4
0 .9 8 8
0 .6 7 3
0 .6 4 6
1 .1 6 4
1 .1 8 3
0 .4 7 2
0 .4 8 8
0 .3 8 5
0 .3 7 6
0 .7 9 2
0 .7 7 2
0.561
0 .5 9 2
0 .9 4 2
0 .9 3 3
1 .4 0 4
1 .5 2 7
0 .9 3 3
0 .9 4 8
0 .4 6 2
0 .4 7 9
0.35
0 .3 6 6
0.74
0 .6 9 9
0 .8 1 8
0.821
0 .8 3 7
0 .8 5 9
1 .6 0 4
1 .4 8 3
0 .8 2 9
0 .8 2 4
0.45
0 .4 6
0 .4 6 8
0 .4 5 5
0.821
0 .8 6 5
0 .8 3 2
0 .8 2 6
0 .9 1 6
0 .9 5 4
1 .1 6 4
1 .1 3 2
0 .8 9 3
0.951
0 .4 5 5
0 .4 6 8
0 .4 3 2
0.41
0.821
0 .8 4 5
0 .9 9 4
1 .0 3 9
1 .2 6 8
1 .5 1 7
0 .4 8 3
0 .5 2 5
0 .7 7 9
0 .7 8 5
0 .9 1 9
0 .8 7
0 .5 5 3
0.511
d ilu tio n
20 20 20 20 20 60 60 20 20 20 20 20 60
60 20 20 20 20 20 60
60 20 20 20 20 20 60
60 20 20 20 20 20 60 60 20 20 20 20 20 60
60 20 20 20 20 20 60
60 20 20 20 20 20 60
60 20 20 20 20 20 60
60 20 20 20 60 20 20 60
P rotein conc (m g/m l) 1 9 .4 8 1 3 .8 6 2 1 .4 5 29.81 2 4 .4 0 3 8 .8 8 3 9 .1 8 2 2 .1 6 2 6 .9 5 1 9 .6 2 29.71 1 8 .3 0 3 4 .3 2 3 4 .4 4 1 3 .8 7 1 0 .9 8 1 7 .9 2 2 2 .9 0 1 3 .8 4 2 8 .0 8 3 0 .6 6 11.68 1 7 .2 4 1 4 .5 0 1 7 .7 5 16.01 3 1 .6 8 3 5 .6 7 1 2 .2 8 9 .8 6 1 3 .4 4 2 5 .9 2 2 0 .7 8 2 9 .3 7 29.91 1 1 .7 6 1 5 .9 8 1 9 .8 2 1 3 .1 9 2 3 .4 7 2 8 .8 0 2 2 .8 3 1 5 .6 4 1 1 .5 3 1 8 .7 5 29.31 18.81 2 8 .2 3 2 1 .4 8 1 4 .3 9 1 6 .3 9 1 6 .9 6 3 0 .8 7 1 6 .5 3 2 7 .3 0 2 7 .6 9 1 6 .8 6 1 6 .5 8 1 8 .7 0 2 2 .9 6 1 8 .4 4 2 7 .6 9 2 5 .2 6 1 6 .6 6 2 0 .3 3 2 7 .8 5 3 0 .2 4 1 5 .6 4 1 7 .8 9 3 1 .9 2
Page A136 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Amount isolated liver microsomes
group# m ouse#
1 1 1 1 1 1 1 1 1 1 AVERAGE
SD 2 2 2 2 2 2 2 2 2 2
AVERAGE SD
3 3 3 3 3 3 3 3 3 3
AVERAGE SD
4 4 4 4 4 4 4 4 4 4
AVERAGE SD
5 5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6 6
AVERAGE SD
7 7 7 7 7 7 7 7 7
7 AVERAGE
SD
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
Protein conc (m g/m l)
m icrosom es
m icrosom es
7 alpha hydroxylase H M G C o A reduct.
19.48
16.94
22.16
15.64
13.87 11.68
16.17 11.87
11.76
12.50
12.28
14.23
15.64
13.94
14.39
13.05
16.86
22.46
16.66
16.18
estim ated pl m icrosom es
600 600 600 600 600 600 600 600 600 600
m g m icrosom es 7 alpha hydroxylase
11.688 13.296 8.322 7.008 7.056 7.368 9.384 8.634 10.116 9.996
17.24 9.86 13.86 26.95 15.98 11.53 10.98 16.39 16.58 20.33
15.25 10.40 14.64 20.92 20.21 12.37 13.57 16.20 15.02 16.01
600 600 600 600 600 600 600 600 600 600
10.344 5.916 8.316 16.17 9.588 6.918 6.588 9.834 9.948 12.198
21.45 19.62 17.92 14.5 13.44 19.82 18.75 16.96 18.7 15.64
21.54 16.06 14.57 14.47 16.68 19.71 23.25 16.91 18.26 12.86
600 600 600 600 600 600 600 600 600 600
12.87 11.772 10.752
8.7 8.064 11.892 11.25 10.176 11.22 9.384
29.81 29.71 22.9 17.75 25.92 13.19 29.31 30.87 22.96 27.85
29.45 34.70 17.64 22.85 25.31 15.59 25.69 31.83 29.73 23.73
700 700 700 700 700 600 700 700 700 700
20.867 20.797 16.03 12.425 18.144 7.914 20.517 21.609 16.072 19.495
13.84 16.01 20.78 23.47 24.4 18.3 18.81 16.53 18.44 17.89
13.41 14.86 23.12 17.91 19.04 20.87 13.69 16.13 20.14 21.40
600 600 600 600 600 600 600 600 600 600
8.304 9.606 12.468 14.082 14.64 10.98 11.286 9.918 11.064 10.734
38.88 34.32 28.08 31.68 29.37 28.8 28.23 27.3 27.69 30.24
39.39 38.61 38.43 32.51 34.40 23.65 34.09 36.46 36.99 32.97
700 700 700 700 700 700 700 700 700 700
27.216 24.024 19.656 22.176 20.559 20.16 19.761 19.11 19.383 21.168
39.18 34.44 30.66 35.67 29.91 22.83 21.48 27.69 25.26 31.92
40.26 42.00 35.84 37.17 38.82 24.81 34.64 38.04 23.73 31.27
700 700 700 700 700 700 700 700 700 700
27.426 24.108 21.462 24.969 20.937 15.981 15.036 19.383 17.682 22.344
m g m icrosom es H M G C o A reduct.
10.164 9.384 9.702 7.122
7.5 8.538 8.364 7.83 13.476 9.708
9.15 6.24 8.784 12.552 12.126 7.422 8.142 9.72 9.012 9.606
12.924 9.636 8.742 8.682 10.008 11.826 13.95 10.146 10.956 7.716
20.615 24.29 12.348 15.995 17.717 9.354 17.983 22.281 20.811 16.611
8.046 8.916 13.872 10.746 11.424 12.522 8.214 9.678 12.084 12.84
27.573 27.027 26.901 22.757 24.08 16.555 23.863 25.522 25.893 23.079
28.182 29.4
25.088 26.019 27.174 17.367 24.248 26.628 16.611 21.889
isolated m icrosom es (m g)
21.85 22.68 18.02 14.13 14.56 15.91 17.75 16.46 23.59 19.70 18.47 3.37 19.49 12.16 17.10 28.72 21.71 14.34 14.73 19.55 18.96 21.80 18.86 4.72 25.79 21.41 19.49 17.38 18.07 23.72 25.20 20.32 22.18 17.10 21.07 3.15 41.48 45.09 28.38 28.42 35.86 17.27 38.50 43.89 36.88 36.11 35.19 8.44 16.35 18.52 26.34 24.83 26.06 23.50 19.50 19.60 23.15 23.57 22.14 3.42 54.79 51.05 46.56 44.93 44.64 36.72 43.62 44.63 45.28 44.25 45.65 4.74 55.61 53.51 46.55 50.99 48.11 33.35 39.28 46.01 34.29 44.23 45.19 7.58
used liver
m g m icrosom es
fo r isolation (g)
pe r g liver
1.92 1.76 1.45 1.19 1.49 1.48 1.42 1.6 1.52 1.31
2.52 1.04 1.8 2.23 1.48 1.05 1.16 1.67 1.34 1.79
1.76 1.98 1.97 1.5 1.39 1.78 1.67 1.35 2.18 1.14
3.1 3.33 3.33 3.53 3.89 1.72 3.21 3.43 3.64 3.19
1.71 1.51 2.32 1.47 2.14 2.17 1.32 1.65 1.62 1.86
3.49 4.36 4.1 4.17 4.13 3.13 2.78 3.57 4.17 3.85
3.85 3.86 3.27 3.92 3.7 3.48 3.27 3.36 2.92 3.48
11.38 12.89 12.43 11.87 9.77 10.75 12.50 10.29 15.52 15.04 12.24 1.89 7.74 11.69 9.50 12.88 14.67 13.66 12.70 11.71 14.15 12.18 12.09 2.12 14.66 10.81 9.90 11.59 13.00 13.32 15.09 15.05 10.17 15.00 12.86 2.10 13.38 13.54 8.52 8.05 9.22 10.04 11.99 12.80 10.13 11.32 10.90 2.00 9.56 12.27 11.35 16.89 12.18 10.83 14.77 11.88 14.29 12.67 12.67 2.13 15.70 11.71 11.36 10.78 10.81 11.73 15.69 12.50 10.86 11.49 12.26 1.88 14.44 13.86 14.24 13.01 13.00 9.58 12.01 13.69 11.74 12.71 12.83 1.45
AVERAGE SD
g roup 1-7 12.26 1.97
Page A137 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Cholesterol-7-a-hydroxylase activity measurements in microsomes
used per assay 420 |jg protein 20 min incubation
internal standard 20 OH: 0.5 jg per determination
Mouse 1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix
+ NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix
+ NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
7.489 7.764 8.344 7.459 7.921 8.048 7.859 8.072 8.614 8.284 8.279 7.708 7.956 8.246 8.569 8.034 8.103 8.551 8.177 7.462 8.615 8.176 7.997 8.678 7.153 8.014 7.760 7.119 8.211 8.341 7.684 7.654 8.062 8.155 8.529 8.412 8.674 8.281 9.215 7.851 8.260 8.138 9.023 9.148 8.783 7.856 7.946 7.525 7.842 7.885 8.094 7.994 7.866 7.808
area 7aOH
1.417 1.459 0.055 2.470 2.542 0.046 2.287 2.372 0.036 0.709 0.725 0.012 2.363 2.406 0.054 0.580 0.602 0.017 0.525 0.452 n.d. 2.048 2.039 0.036 0.912 0.988 0.018 1.032 1.302 0.026 0.389 0.369 n.d. 1.034 0.991 0.027 0.344 0.332 n.d. 0.414 0.408 n.d. 2.248 2.242 n.d. 2.554 2.519 0.055 2.020 2.034 0.031 0.878 0.863 n.d.
|jg 7aOH formed
0.095 0.094 0.003 0.166 0.160 0.003 0.146 0.147 0.002 0.043 0.044 0.001 0.149 0.146 0.003 0.036 0.037 0.001 0.032 0.030 0.000 0.125 0.127 0.002 0.064 0.062 0.001 0.072 0.079 0.002 0.025 0.024 0.000 0.063 0.058 0.002 0.020 0.020 0.000 0.026 0.025 0.000 0.125 0.123 0.000 0.163 0.159 0.004 0.129 0.129 0.002 0.055 0.055 0.000
7 alpha hydroxylase activity (nmol/h/mg protein) 1.681 2.959 2.662 0.785 2.661 0.658 0.576 2.296 1.137 1.373 0.456 1.093 0.368 0.472 2.282 2.898 2.346 1.014
Page A138 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix
+ NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix
+ NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
8.173 7.392 7.547 7.601 8.694 8.113 8.815 8.171 8.490 8.332 8.091 8.957 8.472 7.915 8.828 8.352 8.133 8.018 7.148 8.432 6.831 8.519 8.106 8.352 8.875 7.996 8.907 8.561 9.027 9.222 8.466 8.414 8.950 7.978 8.371 9.549 8.359 7.939 7.754 7.600 8.216 7.859 8.187 7.860 7.683 9.259 8.480 8.305 7.459 8.254 8.066 8.428 8.346 7.805 8.127 8.333 8.348 7.974 7.537 8.761
area 7aOH
3.471 2.911 0.024 0.537 0.542 n.d. 0.556 0.583 n.d. 2.127 2.288 n.d. 1.862 1.780 0.029 1.753 1.984 n.d. 0.368 0.396 n.d. 2.052 1.990 n.d. 0.496 0.455 n.d. 0.678 0.653 n.d. 1.697 1.674 0.029 6.870 6.821 0.075 1.183 1.091 0.011 0.546 0.559 n.d. 2.021 1.895 0.020 0.619 0.595 0.024 1.066 1.116 n.d. 2.606 2.667 0.010 3.369 3.371 n.d. 2.884 2.681 0.043
gg 7aOH formed
0.212 0.197 0.002 0.035 0.031 0.000 0.032 0.036 0.000 0.128 0.141 0.000 0.110 0.112 0.002 0.105 0.122 0.000 0.026 0.023 0.000 0.120 0.123 0.000 0.028 0.028 0.000 0.040 0.036 0.000 0.100 0.099 0.002 0.431 0.407 0.004 0.071 0.069 0.001 0.036 0.034 0.000 0.123 0.121 0.001 0.033 0.035 0.000 0.071 0.068 0.000 0.155 0.160 0.001 0.207 0.202 0.000 0.181 0.178 0.002
7 alpha hydroxylase activity (nmol/h/mg protein) 3.751 0.614 0.621 2.485 2.023 2.096 0.455 2.246 0.521 0.700 1.815 7.668 1.275 0.646 2.229 0.633 1.284 2.892 3.783 3.268
Page A139 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
7.543 7.412 8.080 8.523 8.450 7.586 8.189 9.455 7.871 9.543 8.293 8.583 6.707 8.792 8.695 7.664 8.129 9.510 9.979 9.559 9.372 8.482 9.335 7.948 8.375 8.607 9.266 7.959 10.692 8.987 8.115 9.262 8.761 4.538 4.178 4.434 3.873 4.184 4.643 8.343 9.026 9.171 3.989 3.669 4.843 4.001 4.331 5.012 3.910 4.155 5.027 4.100 4.005 4.765 4.192 4.391 4.288 4.084 4.075 4.527 4.149 3.944 4.428
area 7aOH
4.629 4.514 0.158 4.801 4.542 0.494 0.921 0.949 0.014 1.148 0.974 0.026 3.465 4.504 0.064 6.853 7.362 n.d. 2.836 2.618 n.d. 0.848 0.932 n.d. 4.765 4.853 0.083 0.895 1.034 0.006 0.890 0.924 0.028 2.079 2.064 0.049 1.823 1.740 0.032 4.317 4.463 0.031 0.463 0.409 n.d. 1.789 1.905 0.014 0.450 0.453 n.d. 0.821 0.866 0.037 2.296 2.352 0.023 2.755 2.820 n.d. 1.052 1.025 0.036
|jg 7aOH formed
0.307 0.305 0.010 0.282 0.269 0.033 0.056 0.050 0.001 0.060 0.059 0.000 0.258 0.256 0.004 0.447 0.453 0.000 0.142 0.137 0.000 0.050 0.050 0.000 0.284 0.282 0.004 0.056 0.048 0.000 0.055 0.050 0.000 0.229 0.247 0.006 0.235 0.208 0.003 0.259 0.247 0.002 0.058 0.056 0.000 0.224 0.220 0.001 0.058 0.055 0.000 0.100 0.108 0.004 0.274 0.268 0.003 0.337 0.346 0.000 0.127 0.130 0.004
7 alpha hydroxylase activity (nmol/h/mg protein) 5.466 4.482 0.967 1.098 4.684 8.312 2.577 0.923 5.149 0.960 0.967 4.295 4.031 4.642 1.051 4.071 1.035 1.852 4.954 6.311 2.296
Page A140 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse 39 49 59 69 10 20 40 60 30 50 70
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
4.145 4.132 3.371 4.812 3.305 4.276 3.994 3.969 4.551 3.328 3.792 10.237 9.887 11.010 8.101 9.894 12.152 11.089 10.748 10.280 11.854 9.866 10.231 11.422 10.852 10.771 11.044 10.087 10.560 10.687 10.035 10.174 10.971
n.d.: not detectable
area 7aOH
0.417 0.483 n.d. 1.356 0.979 n.d. 0.219 0.210 n.d. 0.710 0.735 n.d. 3.986 4.342 0.026 3.083 3.063 0.073 0.839 0.883 n.d. 0.951 0.893 0.040 2.471 2.346 n.d. 4.003 3.663 n.d. 1.536 1.606 n.d.
gg 7aOH formed
0.050 0.058 0.000 0.141 0.148 0.000 0.027 0.026 0.000 0.107 0.097 0.000 0.202 0.197 0.002 0.156 0.126 0.003 0.039 0.043 0.000 0.048 0.044 0.002 0.114 0.109 0.000 0.198 0.173 0.000 0.077 0.079 0.000
7 alpha hydroxylase activity (nmol/h/mg protein) 1.004 2.669 0.498 1.880 3.654 2.542 0.757 0.816 2.057 3.435 1.436
Page A141 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
G roup
i
AVERAGE SD 2 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3 3
AVERAGE SD 4 4 4 4 4 4 4 4 4 4
AVERAGE SD 5 5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6 6
AVERAGE SD 7 7 7 7 7 7 7 7 7 7
AVERAGE SD
m ouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
liver w e ight (g)
2 .1 9 2 .0 0 1.66 1.41 1.68 1.70 1.61 1.77 1.68 1.49
2.77 1.17 1.88 2 .5 3 1.68 1.23 1.35 1.84 1.53 1.98
2 .0 0 2 .2 3 2.27 1.68 1.56 2 .0 6 1.85 1.51 2.42 2 .1 4
3 .4 6 3 .6 8 3 .6 9 3 .9 3 4 .2 3 1.94 3.62 3 .9 0 4 .0 3 3 .3 9
1.90 1.71 2 .4 8 1.69 2 .2 5 2 .4 6 1.47 1.88 1.82 2 .1 4
3 .9 0 4.87 4 .1 0 4.17 4 .1 3 3 .4 8 3 .1 3 4.11 4 .7 4 4 .3 3
4 .2 9 4 .3 3 3 .8 8 4 .2 8 4 .1 5 3 .8 5 3.91 3 .8 3 3 .3 3 3.92
average mg m ic ro so m e s/g liver
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
cholestero l 7 alpha hydroxylase a ctivity (nm o l/h /m g protein) per liver (nm ol/h)
1.681
4 5 .1 4
2 .2 9 6 2.282 2 .4 8 5 2.892
56.32 46.47 42.97 5 9 .5 9
1.815 4 .6 8 4
3 7 .8 3 9 2 .4 9
4 .2 9 5 4 .9 5 4 3 .6 5 4
9 3 .2 4 1 0 2 .0 7 6 6 .7 6
3 .1 0 4 1.206
6 4 .2 9 2 3 .5 5
2 .0 2 3
6 8 .7 4
7 .6 6 8 2 .9 5 9
1 1 0 .0 2 68.22
1.137 3 .7 8 3 8.312 2 .8 9 8
35.27 7 7 .9 4 1 2 5 .3 9 4 7 .9 8
4.031 6.311 2.542
9 0 .9 6 1 1 8 .4 3 6 1 .7 4
4 .1 6 6 2.442
80.47 3 0 .1 6
2.662
65.31
1.373 2 .3 4 6
3 7 .5 5 6 5 .3 0
2 .0 9 6 1.275 3 .2 6 8 2.577 4.642 2 .2 9 6 2.057
4 3 .1 9 2 4 .4 0 8 2 .5 6 5 8 .4 8 85.97 6 8 .1 5 5 4 .0 0
2 .4 5 9 0 .9 6 5
5 8 .4 9 19.37
0 .7 8 5 0 .4 5 6
33.32 2 0 .6 0
1.014 0 .4 5 5 0 .6 4 6 5 .4 6 6
4 5 .8 9 21.91 33.51 1 3 0 .0 6
0 .9 2 3 1.051
40.97 5 0 .2 6
1.004 0.757
4 9 .6 5 3 1 .4 8
1.256 1.495
45.77 3 1 .4 0
3.751 2 .2 4 6
8 7 .4 0 47.11
2 .2 2 9 4.482
67.81 92.91
2.661 1.093 5 .1 4 9 4.071
7 3 .4 3 3 2 .9 6 9 2 .8 3 9 3 .8 6
2 .6 6 9 3 .4 3 5
5 9 .5 9 9 0 .1 5
3 .1 7 9 1.221
7 3 .8 0 2 1 .6 0
0 .6 5 8
3 1 .4 8
0 .3 6 8 0 .6 1 4 0.521 0 .6 3 3 0.967 0 .9 6 0
2 2 .0 0 3 0 .8 8 2 6 .6 4 3 2 .0 5 4 1 .2 5 3 6 .8 4
1.035 0 .4 9 8 0 .8 1 6
52.17 2 8 .9 3 4 3 .3 3
0.707 0 .2 2 6
3 4 .5 6 8.94
0 .5 7 6
30.32
0.472 0.621
2 5 .0 5 2 9 .5 4
0 .7 0 0 1.284 1.098 0.967
3 6 .7 4 65.37 5 1 .8 4 4 6 .3 8
1.852 1.880 1.436
8 6 .9 8 7 6 .8 0 6 9 .0 3
1.089 0 .5 1 6
51.81 2 1 .7 9
I
Page A142 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
HMG CoA reductase activity measurements
internal standard mix
i i
i i i i i i
i i
i i i i i i i i
i i i i i i i i
i i
i i i i i i
i i
i i i i i i i i
i i i i i i i i
i i
i 2 2 2 2 2
mouse# i 13 21 31 45 51 61 2 14 22 32 46 52 3 17 23 33 53 4 11 24 34 42 54 64 6 12 25 35
blanc blanc
3H dpm (internal standard)
14802 15138
14416 16500 16182 18200 16197 16086
15302 17401
19233 18151 18408 16484 14705 17314 no sample 19044
17259 17962 16815 18678 17627 16776 18636 13567
12055 12101
13870 12322 13846 15599 13354 14885
17744 17935
15926 15147 17598 18276 17701 13443 16084 13092
12500 12547 12475 15313 15836 11436 13617 15941
12877 13025
17786 15984 16769 15482 14676 12984
CM 5 CO CM
14C dpm
222 207 199 294 310 278 437
187 224 296 360 192 169 132 159
172 124 137 205 265 140 158 226 146 118 92 143 138 116 144 197
231 266 190 172 221 316 187 156 260 209 127 116 114 145 119 86 104 110 196 254 405 279 378 214 162 118
14C dpm 14C mevalonate -blanc formed (dpm)
82 169 67 135
59 125 154 284 170 320 138 231 297 558 181 342
47 93 84 147
156 247 220 369 52 86 29 54 -8 -17 19 33
HMG CoA activity pmol/min/mg protein
5.111 4.083
3.776 8.611 9.692 6.995 16.917 10.381
2.834 4.454
7.483 11.182 2.606 1.623 -0.502 1.012
32 51
-16 -28 -3 -5 65 118 125 204 00 18 33 86 140 6 13
-22 -56 -48 -121
37 -2 -5 -24 -53 48 57 130 101 207
91 156 126 214
50 96 32 64 81 140 176 293 47 81 16 36 120 227 69 160
-13 -32 -24 -58 -26 -63 5 10 -21 -40 -54 -144 -36 -80 -30 -57
56 132 114 266
265 453 139 247 238 403 74 136
1.550
-0.855 -0.154 3.566 6.174 0.000 0.990 4.257 0.408
-1.684 -3.659
0.200 -0.150 -1.599 0.237 3.938 6.260
4.731 6.481
2.896 1.949 4.246 8.885 2.450 1.098 6.883 4.862
-0.959 -1.765 -1.923 0.301 -1.223 -4.356 -2.439 -1.736
4.012 8.075
13.746 7.482 12.211 4.112
mean 4.60 6.19 8.34 13.65 3.64 9.33 2.11 0.26 1.55 0.00 4.87 0.49 2.33 0.00 0.02 0.00 5.10 5.61 2.42 6.57 1.77 5.87 0.00 0.00 0.00 0.00 6.04 10.61 8.16
Input internal standard (dpm): average
mix 1 30176 29765 31362
30434
|
mix 2 28810 28307 28031
28383
14C dpm HMG CoA mix: I average
HMG CoA mix:
2.6
Specific activity 14C HMGCoA: 4398.46
incubation time:
30.0
liver microsomes: 0.250
mmol/l HMG CoA dpm/nmol
min mg
58465 57242 55833
57180 11436
per 5 pl mix per pl mix
Page A143 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mouse# 43 55 65 5 15 26 36 44
66
7 16 27 37 blanc blanc
3Hdpm (internal standard)
16023 14382 15354 16171 15593 15352 13631 12996 13907 11471 10075 12964 13289 16215 17106 14863 14921 14914 14706 15107 13433 14340 14736 15759 16357 15923 14228 11628
Input internal standard(dpm): average [
27932 27694 28968 28198
14Cdpm
252 192 261
211
451 136 140 139 98 109 97 93 139 173 402 338 155 168 195 321 191 287 142 154 204 179 194 144
14Cdpm 14Cmevalonate HMGCoAact.
-blanc formed(dpm) pmol/min.mgeiwit
83 146
4.237
23 45
1.308
92 169
4.901
42 73
2.124
282 510
14.792
-33 -61
-1.758
-29 -60
-1.740
-30 -65
-1.888
-71 -144
-4.176
-60 -147
-4.278
-72 -202
-5.845
-76 -165
-4.795
-30 -64
-1.846
47
0.202
233 384
11.141
169 321
9.300
-14 -26
-0.767
-1 -2
-0.055
26 50
1.446
152 284
8.230
22 46
1.340
118 232
6.731
-27 -52
-1.499
-15 -27
-0.779
35 60
1.750
10 18
0.514
mean 2.77 3.51 6.52
0.00 0.00 0.00 0.00 10.22 0.00
4.84
0.00 0.00
1.13
14CHMGCoAmix: average
58407 55714 65147 59756 11951.2
per5pl mix perpl mix
HMGCoAmix:
2.6mmol/l HMGCoA
Specificactivity14CHMGCoA: 4596.62 dpm/nmol
incubationtime: 30.0
min
liver microsomes: 0.250
mg
mouse# 67 8 9 19 29 39 49 59 50 70
blanc blanc
3H dpm (internal standard)
16112 16092 14295 17696 17286 16982 14651 19083 16755 16504 17225 17750 18651 15076 15831 16644 16807 14453 16679 15823 16121 16049
Input internal standard (dpm): average [
27956 27723 28126
27935
14C dpm
284 180 129 164 379 317 145 113 142 115 247 218 209 152 159 154 195 321 191 287 95 72
14C dpm -blanc 201 97 46 81 296 234 62 30 59 32 164 135 126 69 76 71 112 238 108 204
14C mevalonate formed (dpm) 348 168 89 127 478 384 117 43 98 53 265 212 188 127 133 118 185 459 180 359
HMG CoA act. pmol/min.mg eiwit
9.646 4.648 2.467 3.526 13.251 10.658 3.254 1.198 2.706 1.479 7.358 5.874 5.216 3.522 3.697 3.283 5.142 12.738 4.996 9.969
mean 7.15 3.00 11.95 2.23 2.09 6.62 4.37 3.49 8.94 7.48
14C HMG CoA mix: average
63731 60065 63603
62466 12493.27
per 5 pl mix per pl mix
HMG CoA mix: Specific activity 14C HMGCoA:
incubation time: liver microsomes:
2.6 4805.1
30.0 0.250
mmol/l HMG CoA dpm/nmol
min mg
Page A144 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mouse# 18 28 38 48 58 68 69 10 20 40 30 62 41 63 56 47
blanc
3H dpm (internal standard)
18621 18163 16885 17274 15495 18485 15821 17041
19175 17310 16607 14530 16586 14581 15189 15445
16021 16747 15999 14205 20405 19844 16228 14978
15343 13316 15166 16339 17431 14614 15456 15823 16766
Input internal standard (dpm): average
32959 31221 31297
31826
14C dpm
513 361 265 260 682 691 341 312 412 318 241 348 352 360 275 241 224 235 404 355 591 403 328 218 233 317 214 302 407 514 650 287 164
14C dpm 14C mevalonate -blanc formed (dpm)
349 596 197 345 101 190 96 177 518 1064 527 907 177 356 148 276
248 412 154 283 77 148 184 403 188 361 196 428 111 233 77 159
60 119 71 135 240 477 191 428 427 666 239 383 164 322 54 115
69 143 153 366 50 105 138 269 243 444 350 762 486 1001 123 247
HMG CoA act. pmol/min.mg eiwit
17.513 10.135 5.589 5.193 31.238 26.640 10.454 8.115
12.085 8.313 4.333 11.833 10.592 12.561 6.829 4.658
3.499 3.962 14.017 12.564 19.554 11.254 9.443 3.369
4.202 10.736 3.081 7.892 13.026 22.379 29.382 7.264
mean 13.82 5.39 28.94 9.28 10.20 8.08 11.58 5.74 3.73 13.29 15.40 6.41 7.47 5.49 17.70 18.32
14C HMG CoA mix: average
61119 60406 55583
59036 11807.2
per 5 |jl mix per pl mix
HMG CoA mix: Specific activity 14C HMGCoA:
incubation time: liver microsomes:
2.6 4541.23
30.0 0.250
mmol/l HMG CoA dpm/nmol
min mg
Page A145 of A172
Appendix to TNO-report of 3M-02
Final version
G roup
m ouse#
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD
4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
liver w e ig h t
(g) 2.19 2.00 1.66 1.41 1.68 1.70 1.61 1.77 1.68 1.49
average mg m icro so m e s/g liver
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
2.77 1.17 1.88 2.53 1.68 1.23 1.35 1.84 1.53 1.98
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
2.00 2.23 2.27 1.68 1.56 2.06 1.85 1.51 2.42 2.14
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
3.46 3.68 3.69 3.93 4.23 1.94 3.62 3.90 4.03 3.39
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
1.90 1.71 2.48 1.69 2.25 2.46 1.47 1.88 1.82 2.14
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
3.90 4.87 4.10 4.17 4.13 3.48 3.13 4.11 4.74 4.33
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
4.29 4.33 3.88 4.28 4.15 3.85 3.91 3.83 3.33 3.92
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
H M G C oA reductase activity
I
(p m o l/m in /m g protein) per liver (pm o l/m in)
4.60 0.26 0.00
1 2 3 .4 3 6 .2 6 0 .0 0
2.42 0.00
4 1 .8 8 0 .0 0
0.00 4.84 3.00 11.95 5.74
0 .0 0 9 5 .4 9 6 5 .0 3 2 4 6 .2 3 1 0 4 .9 2
3.28 3.77
68.32 78.41
6.57
2 2 2 .9 6
6.04 6.19 1.55
8 6 .6 9 1 4 2 .7 5 4 8 .0 8
0.00 0.00 0.02 13.82
0 .0 0 0 .0 0 0.41 3 1 1 .8 5
2.23 3.73
4 1 .7 6 9 0 .5 6
4.02 4.33
94.51 1 0 4 .2 4
8.34 0.00 0.00 1.77
2 0 4 .5 9 0 .0 0 0 .0 0 3 6 .5 4
10.61 0.00
2 0 3 .0 0 0 .0 0
0.00 5.39 2.09 15.40
0 .0 0 99.81 6 2 .1 0 4 0 4 .1 5
4.36 5.44
1 0 1 .0 2 1 3 3 .1 3
13.65 4.87 5.10 5.87
5 7 8 .9 8 2 1 9 .7 3 2 3 0 .6 7 2 8 2 .9 6
8.16 0.00
4 2 3 .2 7 0 .0 0
1.13 2 8 .9 4 6.62 13.29
5 0 .2 4 1 3 8 3 .6 8 3 2 6 .8 7 5 5 2 .3 8
8.76 8.36
4 0 4 .8 8 3 9 2 .3 9
7.47 0.00 2.77
1 7 3 .9 9 0 .0 0 8 4 .3 0
10.22 3.64
2 1 1 .7 7 100.51
0.49 18.32 9.28 4.37 8.94
14.93 3 3 0 .2 2 2 1 4 .0 0 9 7 .4 8 2 3 4 .5 6
6.55 5.51
1 4 6 .1 8 1 0 4 .4 8
9.33
4 4 6 .2 3
2.33 5.61 0.00 3.51
1 3 9 .2 8 2 8 1 .8 1
0 .0 0 1 7 7 .8 6
17.70 6.15
7 5 5 .2 8 2 3 6 .0 5
10.20 3.49 6.69
5 1 3 .9 3 2 0 2 .8 2 3 5 5 .0 5
6.50 5.01
3 1 0 .8 3 2 1 6 .2 6
2.11
1 1 1 .2 2
6.41 5.49 0.00 6.52 0.00 7.15
3 4 0 .0 7 2 6 0 .9 8
0 .0 0 3 3 1 .5 8
0 .0 0 3 4 2 .6 1
8.08 11.58 7.48
3 7 9 .5 3 4 7 2 .6 0 359.61
5.48 3.71
2 5 9 .8 2 1 6 5 .0 3
04-12-2006
Page A146 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
m ic ro m e s ; m g p e r g liver, 7 -a lp h a -h y d ro x y la s e a c tiv ity a n d H M G C o A re d u c ta s e activity, 3 v a lu e s M a n n -W h itn e y U -te s t
m icro m e s m g /g liver
group 1 vs 2
nn 10 10 0.971
group 1 vs 3
nn
p
10 10 0 .5 2 9
group 1 vs 4
nn
2
10 10 0 .1 4 3
group 1 vs 5
nn
2
10 10 0 .7 3 9
group 1 vs 6
nn 10 10
2 0.971
group 1 vs 7
nn
2
10 10 0 .3 1 5
grou2 2 vs 3
nn
2
10 10 0 .4 3 6
7 -a lp h a act.
10 10 0 .3 5 3 10 10 0 .3 9 3 10 10 0.002 10 10 0 .9 1 2 10 10 < 0.001 10 10 < 0.001 10 10 0 .1 0 5
7 -a lp h a act. pe r liver
10 10 0 .1 9 0 10 10 0 .6 8 4 10 10 0 .0 3 5 10 10 0 .3 5 3 10 10 < 0.001 10 10 0 .2 4 7 10 10 0 .0 8 9
H M G C o A act.
10 10 0.631 10 10 1.000 10 10 0 .0 2 9 10 10 0 .1 6 5 10 10 0 .1 0 5 10 10 0 .1 6 5 10 10 0 .7 9 6
H M G C o A a ct. p e r liv e r 10 10 0 .7 3 9 10 10 1.000 10 10 0 .0 0 7 10 10 0 .1 0 5 10 10 0 .0 0 3 10 10 0 .0 1 9 10 10 0 .7 3 9
m icro m e s m g /g liver
group 2 vs 4
nn
p
10 10 0 .2 1 8
group 2 vs 5
nn
p
10 10 0 .5 7 9
group 2 vs 6
nn
2
10 10 0 .5 7 9
group 2 vs 7
nn
2
10 10 0 .3 1 5
group 3 vs 4
nn 10 10
2 0 .0 3 5
group 3 vs 5
nn
2
10 10 0 .9 1 2
grou2 3 vs 6
nn
2
10 10 0 .7 3 8
7 -a lp h a act.
10 10 < 0.001 10 10 0 .5 2 9 10 10 < 0.001 10 10 < 0.001 10 10 0.002 10 10 0 .1 9 0 10 10 < 0.001
7 -a lp h a act. pe r liver
10 10 0 .0 0 9 10 10 0 .6 8 4 10 10 < 0.001 10 10 0 .0 4 3 10 10 0 .0 5 2 10 10 0 .0 7 5 10 10 0 .0 0 5
H M G C o A act.
10 10 0 .1 9 0 10 10 0 .2 4 7 10 10 0 .2 4 7 10 10 0 .3 1 5 10 10 0 .2 1 8 10 10 0 .2 8 0 10 10 0 .2 1 8
H M G C o A a ct. p e r liv e r 10 10 0 .0 1 5 10 10 0 .2 4 7 10 10 0 .0 1 5 10 10 0 .0 3 5 10 10 0.011 10 10 0 .1 9 0 10 10 0 .0 2 3
m icro m e s m g /g liver
group 3 vs 7
nn
2
10 10 0 .7 9 6
group 4 vs 5
nn
p
10 10 0 .0 6 3
group 4 vs 6
nn
2
10 10 0 .0 2 9
group 4 vs 7
nn
2
10 10 0 .0 2 9
group 5 vs 6
nn 10 10
2 0.481
group 5 vs 7
nn
2
10 10 0.971
grou2 6 vs 7 |
nn
2
10 10 0 .1 4 3
7 -a lp h a act.
10 10 0.001 10 10 0.002 10 10 0 .5 2 9 10 10 0 .5 2 9 10 10 < 0.001 10 10 < 0.001 10 10 0 .1 4 3
7 -a lp h a act. pe r liver
10 10 0.631 10 10 0 .0 1 5 10 10 0 .3 5 3 10 10 0 .3 5 3 10 10 < 0.001 10 10 0 .0 2 9 10 10 0 .1 2 3
H M G C o A act.
10 10 0 .4 3 6 10 10 0.631 10 10 0 .6 8 4 10 10 0 .5 7 9 10 10 0 .9 1 2 10 10 0 .6 8 4 10 10 0 .9 1 2
H M G C o A a ct. p e r liv e r 10 10 0 .0 6 3 10 10 0 .0 4 3 10 10 0.631 10 10 0 .6 8 4 10 10 0 .0 5 2 10 10 0 .0 6 3 10 10 0 .8 5 3
| 2<005
Page A147 of A172
Appendix to TNO-report of 3M-02
Final version
H2. Cholesterol-27-hydroxylase activity in mitochondria
Protein concentration isolated mitochondria
M itochondria
mouse
i 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
measured prot conc (mg/ml)
well 1
well 2
0.772
0.8
0.699
0.699
0.848
0.908
1.508
1.499
0.925
0.973
0.784
0.813
0.865
0.881
1.143
1.162
0.918
0.931
0.945
1.022
1.192
1.104
1.043
1.062
1.26 1.236
1.201
1.204
0.81 0.81
0.57 0.54
0.915
0.949
1.036
1.092
0.699
0.655
1.033
0.942
0.898
0.839
0.576
0.587
0.726
0.672
0.969
0.959
1.532
1.43
0.784
0.852
0.98 0.997
1.201
1.28
0.699
0.658
0.383
0.359
0.931
0.861
1.37 1.235
0.842
0.845
0.945
0.976
0.949
0.901
0.649
0.637
0.648
0.635
0.775
0.799
0.678
0.705
0.757
0.757
0.84 0.843
1.031
0.998
0.754
0.714
0.467
0.443
0.876
0.886
1.171
1.1
0.554
0.554
0.847
0.809
0.578
0.596
0.616
0.596
0.697
0.7
0.728
0.745
1.808
1.903
1.086
0.85
1.261
1.147
0.734
0.766
0.616
0.611
0.691
0.659
0.847
0.847
1.255
1.251
0.76 0.751
0.85 0.796
0.751
0.728
0.837
0.821
0.84 0.809
1.344
1.34
0.769
0.827
0.642
0.598
0.987
0.965
1.039
0.926
dilution
50 50 50 50 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 50 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 100 100 50 50 100
Protein conc (mg/ml) 39.30 34.95 43.90 75.18 47.45 79.85 87.30 57.63 46.23 49.18 114.80 52.63 124.80 120.25 40.50 27.75 46.60 106.40 33.85 98.75 86.85 29.08 34.95 48.20 148.10 40.90 98.85 124.05 33.93 18.55 44.80 130.25 42.18 96.05 92.50 32.15 32.08 39.35 34.58 37.85 84.15 101.45 36.70 22.75 44.05 113.55 27.70 82.80 58.70 30.30 34.93 36.83 185.55 48.40 120.40 75.00 30.68 33.75 42.35 125.30 37.78 82.30 73.95 41.45 41.23 134.20 79.80 31.00 48.80 98.25
04-12-2006
Page A148 of A172
Appendix to TNO-report of 3M-02
Final version
Amount isolated mitochondria
group#
1 i
1 1
1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD
4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD
6 6
6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
mouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
Protein conc
estim a te d pl
m itochondria (m g/m l) m icrosom es
39.30
600
57.63
600
40.50
600
29.08
600
32.15
600
33.93
600
36.70
600
30.30
600
30.68
600
41.45
600
34.95
18.55 34.95 46.23 32.08 22.75 27.75 34.93 33.75 41.23
600
600 600 600 600 600 600 600 600 600
43.90
49.18 46.60 48.20 44.80 39.35 44.05 36.83 42.35 31.00
600
600 600 600 600 600 600 600 600 600
75.18 114.80 106.40 148.10 130.25 34.58 113.55 185.55 125.30 134.20
700 700 700 700 700 600 700 700 700 700
33.85 40.90 42.18 37.85 47.45 52.63 27.70 48.40 37.78 48.80
600 600 600 600 600 600 600 600 600 600
79.85
124.80 98.75 98.85 96.05 84.15 82.80 120.40 82.30 79.80
700
700 700 700 700 700 700 700 700 700
87.30 120.25 86.85 124.05 92.50 101.45 58.70 75.00 73.95 98.25
700 700 700 700 700 700 700 700 700 700
isolated m itochondria (m g) 23.58 34.58 24.30 17.45 19.29 20.36 22.02 18.18 18.41 24.87
20.97 11.13 20.97 27.74 19.25 13.65 16.65 20.96 20.25 24.74
26.34 29.51 27.96 28.92 26.88 23.61 26.43 22.10 25.41 18.60
52.62 80.36 74.48 103.67 91.18 20.75 79.49 129.89 87.71 93.94
20.31 24.54 25.31 22.71 28.47 31.58 16.62 29.04 22.67 29.28
55.90 87.36 69.13 69.20 67.24 58.91 57.96 84.28 57.61 55.86
61.11 84.18 60.80 86.84 64.75 71.02 41.09 52.50 51.77 68.78 64.28 14.24
used liver mg m itochondria
fo r isolation (g)
per g liver
1 .9 2
12.28
1.76
19.64
1.45
16.76
1.19
14.66
1.49
12.95
1.48
13.75
1.42 1.6 1.52
15.51 11.36 12.11
1.31 18.98
14.80
2.89
2.52
8.32
1.04 1.8
10.70 11.65
2.23
12.44
1.48
13.00
1.05
13.00
1.16
14.35
1.67
12.55
1.34
15.11
1 .7 9
13.82
12.49
1 .9 4
1 .7 6
14.97
1.98
14.90
1.97
14.19
1.5 19.28
1.39
19.34
1.78
13.26
1.67
15.83
1.35 2.18
16.37 11.66
1 .1 4
16.32
15.61
2.42
3.1 16.98
3.33
24.13
3.33
22.37
3.53
29.37
3.89
23.44
1.72
12.06
3 .2 1
24.76
3.43
37.87
3.64
24.10
3.19
29.45
24.45
7.03
1.71 11.88
1.51
16.25
2.32
10.91
1.47
15.45
2.14
13.30
2.17
14.55
1.32
12.59
1.65
17.60
1.62 1.86
13.99 15.74
14.23
2.09
3.49
16.02
4.36
20.04
4.1 16.86
4.17
16.59
4.13
16.28
3.13
18.82
2.78
20.85
3.57
23.61
4.17
13.82
3.85
14.51
17.74
3.05
3.85
15.87
3.86
21.81
3.27
18.59
3.92
22.15
3.7 17.50
3.48
20.41
3.27
12.57
3.36
15.63
2.92
17.73
3.48
19.76
18.20
2.99
AVERAGE SD
group 1-3,5 14.28 2.55
AVERAGE SD
group 4 24.45 7.03
AVERAGE SD
group 6,7 17.97 2.95
04-12-2006
Page A149 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Cholesterol-27-hydroxylase activity measurements
used per assay 420 |jg protein 20 min incubation
internal standard 20 OH: 0.5 jg per determination
Mouse energy generating area 20 OH area 27OH
mix added
1 + NADPH mix 10.798 13.936
+ NADPH mix
10.39
13.174
- NADPH mix
7.98
0.136
13 + NADPH mix
11.806
20.962
+ NADPH mix
12.439
19.566
- NADPH mix
8.742
0.125
21 + NADPH mix 11.5 19.885
+ NADPH mix
11.782
16.14
- NADPH mix
8.867
0.126
31 + NADPH mix
9.271
8.456
+ NADPH mix
10.042
9.881
- NADPH mix
8.477
0.038
45 + NADPH mix
12.431
18.514
+ NADPH mix
12.184
17.151
- NADPH mix
8.816
0.07
51 + NADPH mix 10.388 10.217
+ NADPH mix - NADPH mix
10.595 8.259
11.997 0.113
61 + NADPH mix
9.893
11.615
+ NADPH mix
10.305
11.839
- NADPH mix
8.75
0.068
2 + NADPH mix 9.624 11.398
+ NADPH mix - NADPH mix
10.36 8.95
13.32 n.a.
14 + NADPH mix
11.022
14.253
+ NADPH mix
11.6
14.61
- NADPH mix
9.308
0.023
22 + NADPH mix 10.919 15.555
+ NADPH mix
10.512
16.346
- NADPH mix 9.52 0.074
32 + NADPH mix
8.987
8.043
+ NADPH mix - NADPH mix
9.298 8.538
8.372 0.023
46 + NADPH mix 10.614 15.727
+ NADPH mix
10.223
15.178
- NADPH mix
9.715
0.048
52 + NADPH mix
9.353
9.827
+ NADPH mix - NADPH mix
10.539 8.851
9.649 0.071
62 + NADPH mix
10.024
6.711
+ NADPH mix
9.352
6.677
- NADPH mix
8.92
n.a.
3 + NADPH mix 12.459 19.765
+ NADPH mix
11.384
15.393
- NADPH mix
9.006
n.a.
17 + NADPH mix
10.383
12.005
+ NADPH mix
14.276
18.144
- NADPH mix
9.026
n.a.
23 + NADPH mix
9.646
14.544
+ NADPH mix
10.293
14.524
- NADPH mix
7.233
n.a.
33 + NADPH mix
8.216
7.206
+ NADPH mix
8.156
6.744
- NADPH mix
7.185
0.249
|jg 27 OH formed
0.645 0.634 0.009 0.888 0.786 0.007 0.865 0.685 0.007 0.456 0.492 0.002 0.745 0.704 0.004 0.492 0.566 0.007 0.587 0.574 0.004 0.592 0.643 0.000 0.647 0.630 0.001 0.712 0.777 0.004 0.447 0.450 0.001 0.741 0.742 0.002 0.525 0.458 0.004 0.335 0.357 0.000 0.793 0.676 0.000 0.578 0.635 0.000 0.754 0.706 0.000 0.439 0.413 0.017
27- hydroxylase activity (nmol/h/mg protein) 11.659 15.332 14.181 8.715 13.306 9.645 10.656 11.407 11.766 13.689 8.267 13.654 9.007 6.389 13.571 11.209 13.480 7.549
Page A150 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse energy generating area 20 OH area 27OH mix added
41 + NADPH mix + NADPH mix
10.176 9.541
4.943 4.889
- NADPH mix
7.88
n.a.
53 + NADPH mix
7.856
4.981
+ NADPH mix
7.979
4.873
- NADPH mix
7.279
n.a.
63 + NADPH mix
10.998
7.897
+ NADPH mix - NADPH mix
8.797 7.705
6.181 0.086
4 + NADPH mix 9.576
11.39
+ NADPH mix - NADPH mix
10.192 7.719
12.068 n.a.
11 + NADPH mix + NADPH mix
11.137 9.797
8.145 7.601
- NADPH mix
8.556
0.038
24 + NADPH mix 10.407 11.002
+ NADPH mix - NADPH mix
9.642 6.778
11.512 n.a.
34 + NADPH mix
6.577
3.674
+ NADPH mix
9.528
5.095
- NADPH mix
7.252
n.a.
42 + NADPH mix
9.538
11.731
+ NADPH mix
9.074
10.387
- NADPH mix
7.471
0.027
54 + NADPH mix
8.887
6.229
+ NADPH mix
8.267
5.477
- NADPH mix
7.436
n.a.
64 + NADPH mix
8.585
6.457
+ NADPH mix
8.945
6.783
- NADPH mix
6.454
n.a.
6 + NADPH mix 11.823 13.325
+ NADPH mix - NADPH mix
8.846 8.775
9.816 0.057
12 + NADPH mix
9.224
1.784
+ NADPH mix
9.098
1.721
- NADPH mix
9.234
n.a.
25 + NADPH mix
10.72
7.132
+ NADPH mix
9.542
7.327
- NADPH mix
8.864
0.031
35 + NADPH mix
9.856
5.695
+ NADPH mix - NADPH mix
10.84 8.053
4.8 n.a.
43 + NADPH mix 10.178 12.222
+ NADPH mix
10.114
10.741
- NADPH mix
7.765
n.a.
55 + NADPH mix
8.696
5.552
+ NADPH mix
8.713
5.349
- NADPH mix
8.251
0.032
65 + NADPH mix
9.579
6.842
+ NADPH mix - NADPH mix
9.473 8.811
7.217 0.056
5 + NADPH mix 9.569
7.618
+ NADPH mix
9.56
7.922
- NADPH mix
8.442
0.004
15 + NADPH mix
10.061
8.488
+ NADPH mix
10.559
7.934
- NADPH mix
9.105
n.a.
26 + NADPH mix
9.863
7.929
+ NADPH mix
9.469
8.269
- NADPH mix
8.926
n.a.
gg 27 OH formed
0.243 0.256 0.000 0.317 0.305 0.000 0.359 0.351 0.006 0.595 0.592 0.000 0.366 0.388 0.002 0.529 0.597 0.000 0.279 0.267 0.000 0.615 0.572 0.002 0.350 0.331 0.000 0.376 0.379 0.000 0.564 0.555 0.003 0.097 0.095 0.000 0.333 0.384 0.000 0.289 0.221 0.000 0.600 0.531 0.000 0.319 0.307 0.002 0.357 0.381 0.003 0.398 0.414 0.000 0.422 0.376 0.000 0.402 0.437 0.000
27- hydroxylase activity (nmol/h/mg protein) 4.610 5.749 6.458 10.962 6.920 10.396 5.049 10.933 6.297 6.976 10.270 1.767 6.619 4.714 10.450 5.748 6.758 7.499 7.366 7.746
Page A151 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse energy generating area 20 OH area 27OH
mix added
36 + NADPH mix
9.147
4.853
+ NADPH mix
9.469
4.856
- NADPH mix 7.56 0.022
44 + NADPH mix
9.972
8.19
+ NADPH mix
11.897
8.239
- NADPH mix
8.394
n.a.
56 + NADPH mix
9.607
5.319
+ NADPH mix
9.685
4.876
- NADPH mix
8.852
0.035
66 + NADPH mix
9.829
7.898
+ NADPH mix
10.203
7.893
- NADPH mix
9.701
0.042
7 + NADPH mix
10.39
6.813
+ NADPH mix
9.963
7.322
- NADPH mix
9.004
n.a.
16 + NADPH mix
9.887
6.158
+ NADPH mix
9.808
5.884
- NADPH mix
8.749
n.a.
27 + NADPH mix
10.948
8.509
+ NADPH mix
11.307
8.456
- NADPH mix 9.47 0.018
37 + NADPH mix
10.004
6.283
+ NADPH mix
9.859
6.029
- NADPH mix
8.538
n.a.
47 + NADPH mix
9.054
6.579
+ NADPH mix
10.166
8.773
- NADPH mix
9.566
n.a.
57 + NADPH mix
10.482
5.812
+ NADPH mix
9.237
5.663
- NADPH mix
9.439
n.a.
67 + NADPH mix
9.185
8.637
+ NADPH mix
9.321
10.313
- NADPH mix
8.555
0.041
8 + NADPH mix 10.123 14.048
+ NADPH mix
10.395
11.26
- NADPH mix
8.227
n.a.
18 + NADPH mix
9.534
9.63
+ NADPH mix
9.641
8.353
- NADPH mix
7.802
n.a.
28 + NADPH mix 10.422 10.021
+ NADPH mix
10.894
12.138
- NADPH mix
7.384
n.a.
38 + NADPH mix
9.189
3.667
+ NADPH mix
8.998
3.925
- NADPH mix
8.56
n.a.
48 + NADPH mix 10.497 11.218
+ NADPH mix
10.009
9.378
- NADPH mix
8.178
0.01
58 + NADPH mix
8.418
4.05
+ NADPH mix
9.913
4.887
- NADPH mix
8.293
n.a.
68 + NADPH mix
9.401
6.674
+ NADPH mix
10.176
6.558
- NADPH mix
8.704
n.a.
9 + NADPH mix
8.909
7.488
+ NADPH mix
9.904
10.247
- NADPH mix
7.036
n.a.
19 + NADPH mix
6.336
5.573
+ NADPH mix
8.809
7.57
- NADPH mix
6.461
n.a.
29 + NADPH mix
6.725
5.613
+ NADPH mix
9.786
7.53
- NADPH mix
4.961
0.002
|jg 27 OH formed
0.265 0.256 0.001 0.411 0.346 0.000 0.277 0.252 0.002 0.402 0.387 0.002 0.328 0.367 0.000 0.311 0.300 0.000 0.389 0.374 0.001
0.314 0.306 0.000 0.363 0.431 0.000 0.277 0.307 0.000 0.470 0.553 0.002 0.694 0.542 0.000 0.505 0.433 0.000 0.481 0.557 0.000 0.200 0.218 0.000
0.534 0.468 0.001 0.241 0.246 0.000 0.355 0.322 0.000 0.420 0.517 0.000 0.440 0.430 0.000 0.417 0.385 0.000
27- hydroxylase activity (nmol/h/mg protein) 4.792 6.991 4.846 7.244 6.422 5.647 7.026 5.725 7.341 5.392 9.408 11.412 8.666 9.586 3.858 9.251 4.499 6.255 8.660 8.031 7.405
Page A152 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse energy generating area 20 OH area 27OH ^g 27 OH formed 27- hydroxylase activity
mix added
(nmol/h/mg protein)
39 + NADPH mix 8.832 4.203
0.238
4.380
+ NADPH mix 9.279 4.385
0.236
- NADPH mix 8.084 n.a.
0.000
49 + NADPH mix 9.633 6.413
0.333
6.010
+ NADPH mix 11.234 7.141
0.318
- NADPH mix 7.927 n.a.
0.000
59 + NADPH mix 9.799 4.515
0.230
4.419
+- NNAADDPPHH mmiixx 99..509877 4n.7.a6.1
00..204080
69 + NADPH mix 9.836 6.674
0.339
6.322
+ NADPH mix 9.96 6.877
0.345
- NADPH mix 1.235 n.a.
0.000
10 + NADPH mix 9.549 8.229
0.431
8.204
+ NADPH mix 10.004 9.15
0.457
- NADPH mix 7.526 n.a.
0.000
20 + NADPH mix 9.543 7.346
0.385
7.331
+- NNAADDPPHH mmiixx 98..001098 70..043414
00..401032
40 + NADPH mix 7.696 2.057
0.134
2.390
+ NADPH mix 8.096 2.046
0.126
- NADPH mix 7.695 0.01
0.001
60 + NADPH mix 8.639 3.465
0.201
3.828
+ NADPH mix 8.551 3.757
0.220
- NADPH mix 8.104 0.047
0.003
30 + NADPH mix 8.271 5.462
0.330
6.184
+- NNAADDPPHH mmiixx 87..470668 5n.7.a0.4
00..303090
50 + NADPH mix 9.353 8.537
0.456
7.941
+ NADPH mix 9.445 7.619
0.403
- NADPH mix 7.913 n.a.
0.000
70 + NADPH mix 9.568 4.626
0.242
4.849
+ NADPH mix 8.325 4.716
0.283
- NADPH mix 7.941 n.a.
0.000
Page A153 of A172
Appendix to TNO-report of 3M-02
Final version
G roup
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
M ouse#
1 2 3 4 5 6 7 8 9 10
m ouse#
1 2 3 4 5 6 7 8 9 10
liver w e ight
(g) 2 .1 9 2 .0 0 1.66 1.41 1.68 1.70 1.61 1.77 1.68 1.49
average mg m ito ch o n d ria /g liver
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
11 11 12 12 13 13 14 14 15 15 16 16 17 17 18 18 19 19 20 20
2.77 1.17 1.88 2 .5 3 1.68 1.23 1.35 1.84 1.53 1.98
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
21 21 22 22 23 23 24 24 25 25 26 26 27 27 28 28 29 29 30 30
2 .0 0 2 .2 3 2.27 1.68 1.56 2 .0 6 1.85 1.51 2.42 2 .1 4
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
31 31 32 32 33 33 34 34 35 35 36 36 37 37 38 38 39 39 40 40
3 .4 6 3 .6 8 3 .6 9 3 .9 3 4 .2 3 1.94 3.62 3 .9 0 4 .0 3 3 .3 9
2 4 .4 5 2 4 .4 5 2 4 .4 5 2 4 .4 5 2 4 .4 5 2 4 .4 5 2 4 .4 5 2 4 .4 5 2 4 .4 5 2 4 .4 5
41 41 42 42 43 43 44 44 45 45 46 46 47 47 48 48 49 49 50 50
1.90 1.71 2 .4 8 1.69 2 .2 5 2 .4 6 1.47 1.88 1.82 2 .1 4
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
51 51 52 52 53 53 54 54 55 55 56 56 57 57 58 58 59 59 60 60
3 .9 0 4.87 4 .1 0 4.17 4 .1 3 3 .4 8 3 .1 3 4.11 4 .7 4 4 .3 3
17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97
61 61 62 62 63 63 64 64 65 65 66 66 67 67 68 68 69 69 70 70
4 .2 9 4 .3 3 3 .8 8 4 .2 8 4 .1 5 3 .8 5 3.91 3 .8 3 3 .3 3 3.92
17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97
cholestero l 27 hydroxylase a ctivity I (n m o l/h /m g protein) per liver (nm o l/h)
11.66 11.41 13.57
3 6 4 .7 3 2 5 .9 3 2 1 .8
10.96 7.50
2 2 0 .8 1 8 0 .0
10.27 6.42 11.41 8.66 8.20
2 4 9 .4 147.7 2 8 8 .5 2 0 7 .8 1 7 4 .6
10.01 2.22
248.1 7 3 .9
6.92
2 7 3 .8
1.77 15.33 11.77
2 9 .5 4 1 1 .7 4 2 5 .2
7.37 5.65 11.21 8.67
1 7 6 .8 99.2 216.1 2 2 7 .8
8.03 7.33
1 7 5 .5 2 0 7 .3
8.40 3.70
2 2 4 .3 123.2
14.18 13.69 13.48 10.40
405.1 4 3 6 .0 437.1 2 4 9 .5
6.62 7.75
1 4 7 .5 2 2 7 .9
7.03 9.59 7.40 6.18
1 8 5 .6 2 0 6 .8 2 5 5 .9 1 8 9 .0
9.63 3.14
2 7 4 .0 1 0 9 .9
8.72 8.27 7.55 5.05
7 3 7 .3 7 4 3 .8 681.1 4 8 5 .2
4.71 4.79
4 8 7 .5 2 2 7 .3
5.72 3.86 4.38 2.39
5 0 6 .7 3 6 7 .9 4 3 1 .6 198.1
5.54 2.03
4 8 6 .7 1 9 2 .9
4.61 10.93 10.45
125.1 2 6 7 .0 3 7 0 .2
6.99 13.31
1 6 8 .8 4 2 7 .6
13.65 7.34 9.25 6.01 7.94
4 7 9 .8 154.1 2 4 8 .4 156.2 2 4 2 .7
9.05 3.02
2 6 4 .0 1 2 3 .5
9.65
6 7 6 .0
9.01 5.75 6.30 5.75
7 8 8 .2 4 2 3 .5 4 7 1 .8 4 2 6 .6
4.85 5.39
3 0 3 .0 3 0 3 .3
4.50 4.42 3.83
3 3 2 .3 3 7 6 .4 2 9 7 .8
5.94 1.93
4 3 9 .9 167.2
10.66
8 2 1 .5
6.39 6.46 6.98 6.76 7.24 9.41
497.1 4 5 0 .3 5 3 6 .5 5 0 4 .0 501.1 6 6 1 .0
6.25 6.32 4.85
4 3 0 .5 3 7 8 .3 3 4 1 .6
7.13 1.68
5 1 2 .2 1 4 0 .0
04-12-2006
Page A154 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mitochondria; mgpergliverand27-hydroxylaseactivity, pvaluesMann-WhitneyU-test
mitochondria mg/gliver
group1 vs2 group1vs3 group1vs4 group1vs5 group1 vs6 group1vs7 group2vs3
nn
10 10
0.1p65
n
10
n
10
0.4p36
n
10
n
10
I
0.002
nn
10 10
0.7E96
n
0.0p43
n
10
n
10
0.0p19
n
10
n
10
0.0p05
27hydroxylaseact. 10 10 0.247 10 10 0.631 10 10 0.003 10 10 0.353
0.001 10 10 0.004 10 10 0.579
27hydroxylaseact. perliver 10 10 0.579 10 10 0.684 10 10 0.001 10 10 0.971 10
0.002 10 10 <0.001 10 10 0.353
mitochondria mg/gliver
group2vs4 group2vs5 group2vs6 group2vs7 group3vs4 group3vs5 group3vs6
n
10
n
10
<0.p001
n
10
n
10
0.0p75
n
10
n
10
<0.p001
n
10
n
10
<0.p001
n
10
n
10
p
0.002
n
10
n
10
0.2p18
n
10
n
10
0.1p23
27hydroxylaseact. 10 10 0.075 10 10 0.796 10 10 0.063 10 10 0.165 10 10 0.005 10 10 0.684 10 10 0.003
27hydroxylaseact. perliver 10 10 0.003 10 10 0.631 10 10 0.002 10 10 <0.001 10 10 0.015 10 10 0.739 10 10 0.019
mitochondria mg/gliver
group3vs7 group4vs5 1 group4vs6 | group4vs7 group5vs6 group5vs7 group6vs7
n
10
n
10
0.0p52
n
10
n
10
p
0.001
n
10
n
10
0.0p09
n
10
n
10
p 0.011
nn
10 10
p
0.011
n
10
n
10
0.0p05
n
10
n
10
0.7p39
27hydroxylaseact. 10 10 0.043 10 10 0.015 10 10 0.529 10 10 0.075 10 10 0.019 10 10 0.123 10 10 0.035
27hydroxylaseact. perliver 10 10 0.001 10 10 0.009 10 10 0.393 10 10 0.796 10 10 0.009 10 10 <0.001 10 10 0.089
p<005
Page A155 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
I. Measurements in vitro experiment (rat hepatocytes)
Experiment 1 Protein concentration determination
sample 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Measured
dilution average protein conc
protein conc (mg/ml)
(mg/ml)
97.5 4 376.2 90.6
119.5 4 490.0 125.5
172.9 4 626.8
140.5
155.8 4 672.2 180.3
167.7 4 689.6 177.1
131.6 4 505.6
121.2
139.7 4 508.4
114.5
257.5 4 944.4
214.7
176 4 702.0 175
155.8 4 594.8
141.6
155.8 4 555.8
122.1
167.7 4 645.0
154.8
129 4 512.4
127.2
136.1
4 555.2
141.5
170.8 4 695.8
177.1
172.9 4 717.2 185.7
168.7 4 648.6
155.6
177.1
4 685.6
165.7
151.9 4 605.6
150.9
188.9 4 744.8 183.5
190.1
4 840.0
229.9
168.7 4 662.8
162.7
167.7 4 650.8
157.7
229.9 4 896.6 218.4
150.9 4 594.2
146.2
127.2 4 521.2
133.4
172.9 4 649.6
151.9
170.8 4 713.0 185.7
170.8 4 697.8
178.1
163.7 4 669.0
170.8
gg protein per well 125.4 163.3 208.9 224.1 229.9 168.5 169.5 314.8 234.0 198.3 185.3 215.0 170.8 185.1 231.9 239.1 216.2 228.5 201.9 248.3 280.0 220.9 216.9 298.9 198.1 173.7 216.5 237.7 232.6 223.0
sample 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
Measured protein conc (mg/ml)
134.3
127.2
149.9 131.6
202.7 195.7
194.1 216.8
188.7 203.7
173.8 161.3 194.1 216.8 188.7 203.8 173.9 161.3 168.6 173.9 140.8 143.8 139.7 144.8 155.1 147.9
162.4 164.4
164.4 160.3
179.2 169.7
198.4 167.6
209.1 195.1
207 216.8 239.9 255.6 241.1 249.9 182.33 132.7 201.6 195.1 212.4 221.2 210.4 193 211.4 243.3 208.1 205.9
239.9 223.4
213.5 211.4
198.4
181.3 129.7 133.7 156.1 181.3
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 523.0 563.0 796.8 821.8 784.8 670.2 821.8 785.0 670.4 685.0 569.2 569.0 606.0 653.6 649.4 697.8 732.0 808.4 847.6 991.0 982.0 630.1 793.4 867.2 806.8 909.4 828.0 926.6 849.8 759.4 526.8 674.8
pg protein per well 174.3 187.7 265.6 273.9 261.6 223.4 273.9 261.7 223.5 228.3 189.7 189.7 202.0 217.9 216.5 232.6 244.0 269.5 282.5 330.3 327.3 210.0 264.5 289.1 268.9 303.1 276.0 308.9 283.3 253.1 175.6 224.9
Page A156 of A172
Appendix to TNO-report of 3M-02
Final version
s am ple 63 64 63 64 63 64 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 10 7
M easured
dilution a ve ra g e protein conc
protein co n c (m g/m l)
(m g/m l)
171.8 4 729.6 193
146.9 4 575.4 140.8
166.5 4 687.0 177
147.9 4 561.2 132.7
130.7 4 516.8 127.7
135.7 4 563.2 145.9
131.7 4 542.8 139.7
7 7 .6 72.1
4 299.4
153.1
4 626.6
160.2
132.7 4 518.8 126.7
9 6 .4 9 0 .2
4 373.2
118.4 4 487.4 125.3
162.2 4 605.6 140.6
152.9 4 660.8 177.5
165.9 4 682.6 175.4
129.7 4 498.2 119.4
138.7 4 504.0 113.3
261.1 217.1
4 956.4
176.4 4 703.6 175.4
153.9 4 585.2 138.7
154.8 4 549.8 120.1
167.9 4 645.6 154.9
126.1
4 502.8
125.3
133.2 4 547.6 140.6
169 176.1
4 690.2
174.3 4 721.2 186.3
167.9 4 647.6 155.9
176.4 4 682.6 164.9
150 4 598.0 149
188.5 4 745.2 184.1
189.6 4 822.0 2 2 1 .4
166.9 4 657.6
1 6 1 .9
166.9 4 647.6 156.9
230.1
4 897.0
2 1 8 .4
149 4 588.4 145.2
125.3 4 515.2 132.3
172.2 4 648.2 151.9
170.1
4 710.6
185.2
170.1
4 693.0
176.4
1 6 3 .9 4 668.0
170.1
132.3 4 516.8 126.1
148.1
4 557.4
130.6
203.6 4 800.2 196.5 212.1 4 8 1 5 .0 195.4
14 6.2 4 719.2 2 1 3 .4
pg protein pe r w ell 2 4 3 .2 1 9 1 .8 2 2 9 .0 187.1 1 7 2 .3 1 8 7 .7 1 8 0 .9 99.8 2 0 8 .9 1 7 2 .9 1 2 4 .4 1 6 2 .5 2 0 1 .9 2 2 0 .3 2 2 7 .5 166.1 1 6 8 .0 3 1 8 .8 2 3 4 .5 195.1 1 8 3 .3 2 1 5 .2 1 6 7 .6 1 8 2 .5 230.1 2 4 0 .4 2 1 5 .9 2 2 7 .5 1 9 9 .3 2 4 8 .4 2 7 4 .0 2 1 9 .2 2 1 5 .9 2 9 9 .0 196.1 1 7 1 .7 216.1 2 3 6 .9 2 3 1 .0 2 2 2 .7 1 7 2 .3 1 8 5 .8 2 6 6 .7 2 7 1 .7 2 3 9 .7
04-12-2006
Page A157 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A158 of A172
sam ple com poun d concentration
1 control 2 control 3 control 4 control 5 control 6 control 7 control 8 control 9 control 10 c o n tro l 11 c o n tro l 12 c o n tro l 13 P FBS 1 pM 14 P FBS 1 pM 15 PFBS 10 pM 16 PFBS 10 pM 17 P F B S 1 0 0 pM 18 P FBS 1 0 0 pM 19 PFH S 1 pM 20 PFH S 1 pM 21 P F H S 10 pM 22 P F H S 10 pM 23 PFHS 100 pM 24 PFHS 100 pM 25 PFOS 1 pM 26 PFOS 1 pM 27 PFO S 10 pM 28 PFO S 10 pM 29 PFO S 100 pM 30 PFO S 100 pM 31 R o s u v a s ta tin 0 .0 0 3 pM 32 R osuvastatin 0.003 pM 33 R osuvastatin 0.03 pM 34 R osuvastatin 0.03 pM 35 R osuvastatin 0.3 pM 36 R osuvastatin 0.3 pM 37 PFBS 1 pM 38 PFBS 1 pM 39 PFBS 10 pM 40 PFBS 10 pM 41 P F B S 1 0 0 pM 42 PFBS 1 0 0 pM 43 PFH S 1 pM 44 PFH S 1 pM 45 PFHS 10 pM 46 PFHS 10 pM 47 PFHS 100 pM 48 PFHS 100 pM 49 PFOS 1 pM 50 PFOS 1 pM 51 P F O S 10 pM 52 PFO S 10 pM 53 PFO S 100 pM 54 PFO S 100 pM
l4C L a b e l
l4C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid
14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C la u ric a cid 14C la u ric a cid 14C la u ric a cid
14C p a lm itic a cid 14C p a lm itic a cid 14C p a lm itic a cid
14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid
14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid
3H -c h o le s te ro l 14C -c h o le s te ro l
(dpm)
(dpm)
12705 12827
7957 10612
11820
12414
14124
8971
12699 15542
11979 14954
12531
17327
11864
16561
11644
15565
14154
7968
12949 14391
7804 6763
15327 14468
11500 11220
14439
13928
18148
17531
12484
10821
13353
10981
15401
13298
14081
16955
14983
15087
13978
14244
14523
14505
16419 13313
18682 9191
13999
14418
13096
11960
14417
13268
12438
8472
12519
9940
13661
6222
14096
5074
12455
1670
12791
2268
14394
624
13181
465
13006
7667
13189
10370
13331 12774
17500 14299
13454
8093
16489
14468
12397
13939
12966
11630
13703
10974
13864
15549
15672
16480
15719
17411
12626
9783
13507
16521
15475
19586
13164
12135
15165 15228
9834 10776
14C -c h o le s te ro l c o rre c te d for recovery (dpm) 16067 21225 26944 16295 24200 24684 35474 35812 34294 14442 15462 12056 19249 19896 24747 24783 22237 21098 22152 30891 25833 26143 25623 29191 17712 26423 23430 23610 17475 20370 11685 9235 3440 4549 1112 905 15124 20172 33678 28718 15432 22511 28846 23012 20546 28773 26978 28417 19878 31380 32470 23650 16636 18155
pg protein
1 2 5 .4 1 6 3 .3 2 0 8 .9 224.1 2 2 9 .9 1 6 8 .5 1 6 9 .5 3 1 4 .8 234 1 9 8 .3 1 8 5 .3 215 1 7 0 .8 185.1 2 3 1 .9 239.1 2 1 6 .2 2 2 8 .5 2 0 1 .9 2 4 8 .3 280 2 2 0 .9 2 1 6 .9 2 9 8 .9 198.1 1 7 3 .7 2 1 6 .5 2 3 7 .7 2 3 2 .6 2 2 3 .0 1 7 4 .3 1 8 7 .7 2 6 5 .6 2 7 3 .9 2 6 1 .6 2 2 3 .4 2 7 3 .9 2 6 1 .7 2 2 3 .5 2 2 8 .3 1 8 9 .7 1 8 9 .7 202.0 2 1 7 .9 2 1 6 .5 2 3 2 .6 2 4 4 .0 2 6 9 .5 2 8 2 .5 3 3 0 .3 3 2 7 .3 210.0 2 6 4 .5 289.1
14C -c h o le s te ro l per mg protein (dpm /m g)
128130 129974 128982 72713 105265 146495 209286 113761 146556 72832 83441 56077 112700 107486 106714 103650 102856 92331 109717 124411 92261 118349 118134 97661 89408 152118 108220 99328 75127 91345 67038 49200 12951 16608
4251 4051 55216 77079 150685 125790 81351 118664 142802 105606 94899 123702 110564 105442 70366 95004 99207 112617 62898 62797
A v e ra g e 14C -c h o le s te ro l per m g protein (dpm /m g)
129029
108158
156534
70783
110093 105182 97594 117064 105305 107898 120763 103774 83236 58119 14780
4151 66147 138237 100007 124204 109301 108003 82685 105912 62847
% o f control A verage % o f control
9 9 100 101 100 67 100 97 135 134 100 73 94 103 100 118 79
87 85 83 83 82 80 80 76 72 8 5 91 96 72 82 92 92 84 76 69 94 118 84 80 77 58 65 71 52 45 38 10 11 13 33 3 51 61 71 139 128 116 75 92 110 132 115 98 88 101 114 102 100 97 65 76 88 92 98 104 58 58 58
SD 0.7
34.2
31.0
19.5
2.9 1.7 5.8 8.1 14.3 11.2 34.4 4.9 8.9 9.8 2.0 0.1 14.3 16.3 24.4 24.3 18.8 3.3 16.1 8.8 0.1
0 1 o
CD
C/J
II
(-D\
O C/)
r3+
3"
CD
C/)
5'C/)"
-\
0)
CD "D
o0i )i
0 VC
1I
CD C/)
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A159 of A172
sam ple
55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107
co m p o u n d conce ntration
R osuvastatin 0.003 pM R osuvastatin 0.003 pM R o suvastatin 0.03 pM R o suvastatin 0.03 pM
R osuvastatin 0.3 pM R osuvastatin 0.3 pM
PFBS 1 pM PFBS 1 pM P FBS 10 pM P FBS 10 pM PFBS 100 pM PFBS 100 pM PFHS 1 pM PFHS 1 pM PFHS 10 pM PFHS 10 pM PFHS 1 0 0 pM PFHS 1 0 0 pM PFO S 1 pM PFO S 1 pM PFO S 1 0 pM PFO S 1 0 pM PFO S 100 pM PFO S 100 pM R osuvastatin 0.003 pM R osuvastatin 0.003 pM R o suvastatin 0.03 pM R o suvastatin 0.03 pM R osuvastatin 0.3 pM R osuvastatin 0.3 pM PFBS 1 pM PFBS 1 pM P FBS 10 pM P FBS 10 pM PFBS 100 pM PFBS 100 pM PFHS 1 pM PFHS 1 pM PFHS 10 pM PFHS 10 pM PFHS 1 0 0 pM PFHS 1 0 0 pM PFO S 1 pM PFO S 1 pM PFO S 10 pM PFO S 1 0 pM PFO S 100 pM PFO S 100 pM R osuvastatin 0.003 pM R osuvastatin 0.003 pM R o suvastatin 0.03 pM R o suvastatin 0.03 pM R osuvastatin 0.3 pM
14C L a b e l
l4C b u ty ric a c id l4C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id
14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id
14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C p a lm itic a c id in p u t 3H c h o le s te ro l in p u t 3H c h o le s te ro l in put H cholestero l a v e ra g e in p u t 3H c h o le s te ro l
3H -c h o le s te ro l (dpm ) 15609 15687 14378 13730 15870 12832 13019 16614 16141 15873 17295 16841 10210 15012 15875 16131 16397 14452 15496 17004 13663 13951 14919 16126 15126 15117 15845 15353 16583 15158 14410 15961 16275 14927 14899 2959 14864 15988 14366 15716 13749 16009 14012 15284 14474 12042 14571 13686 13478 13285 12506 13476 14963 26014 25923 25028 25655
14C -c h o le s te ro l (dpm ) 4123 4793 901 984 311 512 11827 19187 21002 19906 21050 16813 11716 18465 17804 20213 12748 11356 14299 17756 13984 16405 11557 12122 4914 5462 1201 1756 465 505 5450 5718 5596 5781 5367 1505 6675 4773 5602 8024 5977 5872 4048 9125 5125 5104 4478 4542 3476 2874 1940 1859 1145
14C - c h o le s te ro l c o rre c te d fo r recovery (dpm ) 6777 7839 1608 1839 503 1024 23306 29628 33381 32173 31225 25612 29439 31556 28772 32147 19946 20159 23673 26790 26258 30168 19874 19285 8335 9270 1945 2934 719 855 9703 9191 8821 9936 9242 13049 11521 7659 10004 13098 11153 9410 7412 15317 9084 10874 7884 8514 6616 5550 3980 3539 1963
pg protein
2 6 8 .9 3 0 3 .1 2 7 6 .0 3 0 8 .9 2 8 3 .3 2 5 3 .1 1 7 5 .6 2 2 4 .9 2 4 3 .2 1 9 1 .8 2 2 9 .0 187.1 1 7 2 .3 1 8 7 .7 1 8 0 .9 99.8 2 0 8 .9 1 7 2 .9 1 2 4 .4 1 6 2 .5 2 0 1 .9 2 2 0 .3 2 2 7 .5 166.1 1 6 8 .0 3 1 8 .8 2 3 4 .5 195.1 1 8 3 .3 2 1 5 .2 1 6 7 .6 1 8 2 .5 2 3 0 .1 2 4 0 .4 2 1 5 .9 2 2 8 .5 1 9 9 .3 2 4 8 .4 2 7 4 .0 2 1 9 .2 2 1 5 .9 2 9 9 .0 196.1 1 7 1 .7 2 1 6 .1 2 3 6 .9 2 3 1 .0 2 2 2 .7 1 7 2 .3 1 8 5 .8 2 6 6 .7 2 7 1 .7 2 3 9 .7
14C -c h o le s te ro l per m g protein (d p m /m g )
25201 25861 5825 5952 1775 4044 132722 131739 137258 167745 136354 136891 170860 168120 159051 322115 95480 116594 190300 164859 130053 136939 87357 116105 49611 29076 8292 15040 3925 3972 57894 50361 38336 41330 42805 57105 57807 30833 36511 59756 51657 31472 37795 89207 42036 45901 34131 38232 38401 29871 14922 13026 8190
A v e r a g e 14C -c h o le s te ro l per m g protein (dpm /m g)
25531 5889 2910 132231 152501 136623 168120 240583 106037 177579 133496 101731 39343 11666 3948 54127 39833 42805 44320 48134 41565 63501 43968 36182 34136 13974 8190
% o f control Average % o f control
23 24 24 55 6 23 4 85 84 84 88 97 107 87 87 87 109 108 107 102 154 206 61 68 74 122 113 105 83 85 87 56 65 74 32 25 19 57 10 33 3 82 76 71 54 56 58 6 0 71 81 82 63 44 52 68 84 73 59 44 53 90 126 59 62 65 4 8 51 54 54 48 42 21 20 18 12 12
Appendix to TNO-report of 3M-02 Experiment 2
Final version
04-12-2006
Protein concentration determination
sample 1 2 3 4 5 6 7 8 9 10 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Measured
protein conc (mg/ml) 191.5 204.6 218.1 230.3 234.8 221 267.6 215.2 187.7 214.2 222.9 233.8 235.8 266.6 250 260.3 232.8 241.9 246.9 269.7 211.4 195.2 155.9 154.1 123.5 172.1 156.8 231.8 139.1 114.9 147 194.3 256.2 220.1 126.6 143.5 221 217.1 158.6 179.4 235.8 224.9 175.8 241.8 196.3 204.6 206.6 200.8 240.8 242.9 190.5 191.5 150.6 154.1 142.6 140.9
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 792.2 896.8 911.6 965.6 803.8 913.4 1004.8 1020.6 949.4 1033.2 813.2 620.0 591.2 777.2 508.0 682.6 952.6 540.2 876.2 676.0 921.4 835.2 801.8 814.8 967.4 764.0 609.4 567.0
pg protein per well 264.1 298.9 303.9 321.9 267.9 304.5 334.9 340.2 316.5 344.4 271.1 206.7 197.1 259.1 169.3 227.5 317.5 180.1 292.1 225.3 307.1 278.4 267.3 271.6 322.5 254.7 203.1 189.0
sample 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
Measured
protein conc (mg/ml) 231.9 240.9 211.3 208.5 216.2
239.8 203.7 235.8 292.4 340.6 167.6 197.1 177.5 211.3 237.4 232.4 217.3 244.9 202.1 206.2 240 266.7 181.8 202.9 217.3 223.1 188.5 174.1 238.2 250 173.3 204.6 235.8 231.6 229.9 240 183.5 188.5 163.1 152 202.9 228.1 213.8 244.9 211.4 218.1 168.2 149.4 211.4 213 232.4 254.2 245.8 224 156.2 136.5
215.6
236.6 133.9 158.7 210.5 209.7 156.2 184.3
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 945.6 839.6 912.0 879.0 1266.0 729.4 777.6 939.6 924.4 816.6 1013.4 769.4 880.8 725.2 976.4 755.8 934.8 939.8 744.0 630.2 862.0 917.4 859.0 635.2 848.8 973.2 939.6 585.4 904.4 585.2 840.4 681.0
pg protein per well 315.2 279.9 304.0 293.0 422.0 243.1 259.2 313.2 308.1 272.2 337.8 256.5 293.6 241.7 325.5 251.9 311.6 313.3 248.0 210.1 287.3 305.8 286.3 211.7 282.9 324.4 313.2 195.1 301.5 195.1 280.1 227.0
Page A160 of A172
Appendix to TNO-report of 3M-02
Final version
sam ple 63 64 63 64 63 64 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108
M easured protein conc (m g/m l)
252.5 225.7 153.6 142.5 228.2 210.5 228.1 204.6 220.6 244.2 133.9 150.2 220.6 224.8 234.9 236.6 247.5 230.7 137.3 156.4 206.2 194.7
150 175 152.7 142.8 141.9 140.1 230.3 233.8 237.3 211.6 184 146.9 118.4 200.1 180.4 235.6 162.6 132 211.6 252.4 168.8 170.6 222.3 242.6 257.7 247.1 223.1 208.9 178.7 215.1 179.6 194.7 168.8 142.9 188.5 222.3 196.5 180.4 226.7 240.9
182.2 228.4
240 165.2 142.8 272.7 243.5 201.8 167.1 213.4 236.5 254.2 219.6 153.6
122 232 281.5 207.2 227.6 208.1 207.2 192.9 161.7 281.6 218.7 145.5 151.8 162.6 137.4
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (m g/m l) 252 256 252 256 252 256 276 280 284 288 292 296 300 304 308 312 316 320 324 328 332 336 340 344 348 352 356 360 364 368 372 376 380 384 388 392 396 400 404 408 412 416 420 424 428 432
[jg protein per well 84.0 85.3 84.0 85.3 84.0 85.3 92.0 93.3 94.7 96.0 97.3 98.7 100.0 101.3 102.7 104.0 105.3 106.7 108.0 109.3 110.7 112.0 113.3 114.7 116.0 117.3 118.7 120.0 121.3 122.7 124.0 125.3 126.7 128.0 129.3 130.7 132.0 133.3 134.7 136.0 137.3 138.7 140.0 141.3 142.7 144.0
04-12-2006
Page A161 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A162 of A172
sam ple com pound concentration
1 control 2 control 3 control 4 control 5 control 6 control 7 control 8 control 9 control 13 P F B S 1 |j M 14 P FB S 1 j M 15 P FB S 10 j M 16 P FB S 10 j M 17 P F B S 100 j M 18 P FB S 100 j M 19 P FH S 1 j M 20 P F H S 1 j M 21 P F H S 10 j M 22 P F H S 10 j M 23 PFHS 100 j M 24 PFHS 100 j M 25 PFOS 1 j M 26 PFOS 1 j M 27 P F O S 1 0 j M 28 P F O S 1 0 j M 29 PFO S 100 j M 30 PFO S 100 j M 31 R o s u v a s ta tin 0 .0 0 3 j M 32 R osuvastatin 0.003 j M 33 R osuvastatin 0.03 j M 34 R osuvastatin 0.03 j M 35 R osuvastatin 0.3 j M 36 R osuvastatin 0.3 j M 37 PFBS 1 j M 38 PFBS 1 j M 39 PFBS 10 j M 40 PFBS 10 j M 41 P F B S 1 0 0 j M 42 PFBS 100 j M 43 PFHS 1 j M 44 PFHS 1 j M 45 PFHS 10 j M 46 PFHS 10 j M 47 PFH S 100 j M 48 PFHS 100 j M 49 PFOS 1 j M 50 PFOS 1 j M 51 P F O S 1 0 j M 52 P F O S 1 0 j M 53 PFO S 100 j M 54 PFO S 100 j M
l4C L a b e l
l4C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid
14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid
14C la u ric a cid 14C la u ric a cid 14C la u ric a cid
14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid 14C a c e tic a cid
14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid 14C b u ty ric a cid
3H -c h o le s te ro l 14C -c h o le s te ro l
(dpm )
(dpm )
10176
13013
15067
29921
8013
20565
7102
3628
13239 16182
7313 13946
11285
19393
9341
14887
14585
19635
10415
15531
11106
22304
13209
31195
17775
41007
11729
24081
16244
42002
15747
34491
13331
30717
15569
28923
8199
21682
15486
25911
14084
36299
15835
30330
16929
46461
15411
37008
9454
20098
15895
26782
17054
29629
18199
16110
17403
11966
16235
3127
14014
1836
15647 9439
499 439
17104
8190
16962
23749
16583
11608
15691
16193
14615
17212
16380
17173
16700
12743
15447
20907
15347
18404
15658
22910
16797
19524
16476
25363
14120
11804
13621
21880
16279
28289
16347
17820
15609
18990
16385
28115
14C -c h o le s te ro l c o rre c te d for recovery (dpm ) 34516 53601 69272 13788 14910 23262 46384 43017 36337 40250 54206 63744 62269 55416 69791 59120 62193 50143 71378 45162 69565 51699 74077 64817 57380 45479 46894 23893 18559 5199 3536 861 1255 12924 37791 18894 27855 31788 28298 20596 36532 32368 39492 31373 41550 24616 43357 46904 29423 32838 46314
j g protein
264.1 2 9 8 .9 3 0 3 .9 3 2 1 .9 2 6 7 .9 3 0 4 .5 3 3 4 .9 3 4 0 .2 3 1 6 .5 271.1 2 0 6 .7 197.1 259.1 1 6 9 .3 2 2 7 .5 3 1 7 .5 180.1 292.1 2 2 5 .3 307.1 2 7 8 .4 2 6 7 .3 2 7 1 .6 3 2 2 .5 2 5 4 .7 203.1 1 8 9 .0 3 1 5 .2 2 7 9 .9 3 0 4 .0 2 9 3 .0 4 2 2 .0 243.1 2 5 9 .2 3 1 3 .2 308.1 2 7 2 .2 3 3 7 .8 2 5 6 .5 2 9 3 .6 2 4 1 .7 3 2 5 .5 2 5 1 .9 3 1 1 .6 3 1 3 .3 2 4 8 .0 210.1 2 8 7 .3 3 0 5 .8 2 8 6 .3 2 1 1 .7
14C -c h o le s te ro l per mg protein (dpm /m g)
130711 179328 227969 42839 55647 76402 138487 126446 114821 148487 262288 323464 240359 327262 306730 186184 345324 171682 316765 147042 249876 193434 272742 201004 225315 223922 248115 75803 66313 17101 12069
2040 5163 49863 120662 61317 102332 94102 110338 70149 151125 99450 156757 100685 132635 99258 206398 163241 96218 114684 218739
A v e ra g e 14C -c h o le s te ro l per mg protein (dpm /m g)
179336
58296
126585
205388 281912 316996 265754 244224 198459 233088 213159 236019 71058 14585
3601 85262 81825 102220 110637 128104 116660 152828 129729 166712
% o f control Average % o f control
73 100 100 127 73 100 95 131
83 146 180 134 182 171 104 193 96 177 82 139 108 152 112 126 125 138 42 37 10
7 1 3 86 207 105 176 161 189 120 259 171 269 173 228 170 354 280 165 197 375
115 157 177 148 136 111 130 119 132 40
8 2 146 140 175 190 220 200 262 223 286
SD 27.1
29.1
4 4 .9 3 2 .8 8.1 62.7 57.2 4 0 .5 3 1 .3 9.6 9.5 3.7 2.0 1.2 85.9 49.8 19.7 98.2 6 9 .5 3 8 .8 1 3 0 .0 8 1 .3 126.2
0 1 o
CD CI/JI
(-D\
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5'C/)"
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Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A163 of A172
sa m p le co m p o u n d co n c e n tra tio n
14C L a b e l
3H - c h o le s te ro l 14C - c h o le s te ro l
(dpm )
(dpm )
55 R osuvastatin 0.003 uM 56 R osuvastatin 0.003 uM 57 R o suvastatin 0.03 uM 58 R o suvastatin 0.03 pM
l4C b u ty ric a c id l4C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id
16224 16103 16808 17593
4328 5533 975 1781
59 R osuvastatin 0.3 uM
14C b u ty ric a c id
14906
452
60 R osuvastatin 0.3 uM 61 P F B S 1 uM
14C b u ty ric a c id 14C la u ric a c id
15758 14491
284 19019
62 PFBS 1 uM 63 PFBS 10 uM
14C la u ric a c id 14C la u ric a c id
17266 16567
28110 21487
64 PFBS 10 uM 65 P F B S 100 uM
14C la u ric a c id 14C la u ric a c id
17868 17520
19412 21648
66 P F B S 100 uM 67 PFH S 1 uM
14C la u ric a c id 14C la u ric a c id
19364 16638
28972 26282
68 PFH S 1 uM 69 PFH S 10 uM 70 PFH S 10 uM
14C la u ric a c id 14C la u ric a c id 14C la u ric a c id
17741 17115 16449
30159 25762 23842
71 P F H S 10 0 uM 72 P FH S 100 uM 73 PFO S 1 uM 74 PFO S 1 uM
14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id
15800 16793 16050 17035
21356 27449 18811 24894
75 PFO S 10 uM 76 PFO S 10 uM 77 PFO S 100 uM 78 PFO S 100 uM
14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id
16660 15663 16046 13213
16687 19295 27259 24544
79 R osuvastatin 0.003 uM 80 R osuvastatin 0.003 uM 81 R o s u v a s ta tin 0 .0 3 uM 82 R o suvastatin 0.03 uM
14C la u ric a c id 14C la u ric a c id 14C la u ric a c id 14C la u ric a c id
13776 12989 15098 11184
6081 3529 1767 2114
83 R osuvastatin 0.3 uM 84 R osuvastatin 0.3 uM
14C la u ric a c id 14C la u ric a c id in p u t 3H c h o le s te ro l
13447 14569 26701
566 329
in p u t 3H c h o le s te ro l in p u t 3H c h o le s te ro l 1 a v e r a g e in p u t 3H c h o le s te ro l
27378 26895 269 91.3 3333
14C - c h o le s te ro l c o r re c te d fo r recovery (dpm ) 7200 9274 1566 2732 818 486 38645 43943 35007 29324 33351 40384 42637 45884 40628 39123 36483 44119 31635 39444 27035 33250 45853 50138 11915 7333 3159 5102 1136 610
ug protein
2 8 2 .9 3 2 4 .4 3 1 3 .2 1 9 5 .1 3 0 1 .5 1 9 5 .1 2 8 0 .1 2 2 7 .0 3 1 8 .8 1 9 7 .4 2 9 2 .5 2 8 8 .5 3 0 9 .9 1 8 9 .4 2 9 6 .9 3 1 4 .3 3 1 8 .8 1 9 5 .8 2 6 7 .3 2 1 6 .7 1 9 7 .0 1 8 8 .0 3 0 9 .4 2 9 9 .3 2 2 0 .6 2 1 2 .3 2 7 7 .3 1 9 6 .4 3 0 9 .3 2 2 6 .3
14C - c h o le s te ro l pe r m g p rotein (d pm /m g)
25449 28589 4999 14003 2715 2494 137952 193583 109809 148550 114033 139995 137582 242261 136826 124462 114438 225325 118363 182048 137234 176863 148200 167537 54010 34537 11390 25977 3673 2694
A v e ra g e 14C - c h o le s te ro l pe r m g pro te in (d pm /m g)
27019 9501 2604 165768 129179 127014 168120 130644 169881 150205 157048 157868 44273 18684 3183
% of control A verage % of control
44 46 49 9 16 24 54 4 109 131 153 87 102 117 90 100 111 109 150 191 108 103 98 90 134 178 94 119 144 108 124 140 117 125 132 43 35 27 9 15 21 33 2
SD 3.8 10.9 0.3 31.1 2 1 .6 14.5 5 8 .5 6.9 6 1 .9 3 5 .6 22.1 10.8 10.9 8.1 0.5
Appendix to TNO-report of 3M-02 Experiment 3
Final version
Protein concentration determination
sa m p le
1 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55
M easured
protein conc (m g/m l)
246.35 298.46 229.35 266.32 232.70 299.74 186.54 154.56 143.00 169.33 153.58 301.02 220.50 370.42 222.70 224.91 171.32 272.34 180.40 240.62 227.12 209.66 295.91 248.65 295.91 331.42 268.72 222.71 282.13 236.08 172.33 263.93 175.34 171.32 247.50 249.81 393.62 348.03 289.60 153.58 227.12 209.66 317.98 272.34 211.81 258.00 195.88 304.88 389.03 352.27 258.00 279.67 363.75 295. 9 1
dilution
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
protein conc pg protein
(m g/m l)
per well
985.4
328.5
1193.8
397.9
917.4
305.8
1065.3
355.1
930.8
310.3
1199.0
399.7
746.2 618.2
248.7 206.1
572.0
190.7
677.3
225.8
614.3 1204.1
204.8 401.4
882.0
294.0
1481.7
493.9
890.8
296.9
899.6
299.9
685.3
228.4
1089.4
363.1
721.6
240.5
962.5
320.8
908.5
302.8
838.6
279.5
1183.6
394.5
994.6
331.5
1183.6
394.5
1325.7 1074.9
441.9 358.3
890.8
296.9
1128.5
376.2
944.3
314.8
689.3 1055.7
229.8 351.9
701.4
233.8
685.3
228.4
990.0
330.0
999.2
333.1
1574.5
524.8
1392.1
464.0
1158.4
386.1
614.3
204.8
908.5
302.8
838.6
279.5
1271.9
424.0
1089.4
363.1
847.2 1032.0
282.4 344.0
783.5
261.2
1219.5
406.5
1556.1
518.7
1409.1 1032.0
469.7 344.0
1118.7
372.9
1455.0
485.0
1183.6
394.5
04-12-2006
Page A164 of A172
Appendix to TNO-report of 3M-02
Final version
sample proteiMn ecaosnucre(md g/ml) dilution pro(mtegin/mclo)nc ^gpeprrwoteeliln
56 356.51
4 1426.0 475.3
57 319.45
4 1277.8 425.9
58 356.51
4 1426.0 475.3
59 319.45
4 1277.8 425.9
60 226.01
4 904.0 301.3
61 241.05
4 964.2 321.4
62 333.17
4 1332.7 444.2
63 251.23
4 1004.9 335.0
64 297.09
4 1188.4 396.1
65 232.80
4 931.2 310.4
66 268.15
4 1072.6 357.5
67 294.15
4 1176.6 392.2
68 222.82
4 891.3 297.1
69 295.13
4 1180.5 393.5
70 244.74
4 979.0 326.3
71 237.38
4 949.5 316.5
72 207.61
4 830.4 276.8
73 324.00
4 1296.0 432.0
74 253.09
4 1012.4 337.5
75 241.05
4 964.2 321.4
76 283.46
4 1133.8 377.9
77 267.20
4 1068.8 356.3
78 275.77
4 1103.1 367.7
79 347.64
4 1390.6 463.5
80 216.53
4 866.1 288.7
81 368.75 4 1475.0 491.7
82 219.00
4 876.0 292.0
83 329.09
4 1316.4 438.8
84 221.92
4 887.7 295.9
85 285.39
4 1141.6 380.5
86 348.30
4 1393.2 464.4
87 312.93
4 1251.7 417.2
88 212.95
4 851.8 283.9
89 288.30
4 1153.2 384.4
90 281.53
4 1126.1 375.4
91 305.97 4 1223.9 408.0
92 245.66
4 982.6 327.5
93 274.81
4 1099.2 366.4
94 289.28
4 1157.1 385.7
95 254.03
4 1016.1 338.7
96 230.98
4 923.9 308.0
97 288.30
4 1153.2 384.4
98 332.15
4 1328.6 442.9
99 294.15
4 1176.6 392.2
100 219.22
4 876.9 292.3
101 335.22
4 1340.9 447.0
102 252.16
4 1008.6 336.2
103 297.09
4 1188.4 396.1
104 247.52
4 990.1 330.0
105 280.57
4 1122.3 374.1
106 250.30
4 1001.2 333.7
107 296.11
4 1184.4 394.8
108 217.43
4 869.7 289.9
04-12-2006
Page A165 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A166 of A172
3H cholesterol sam ple m ix 11 13 14 15 16 17 18 1 11 1 12 1 13 1 14 1 15 1 16 1 17 1 18 1 19 1 20 1 21 1 22 1 23 1 24 1 25 1 26 1 27 1 28 1 29 1 30 1 31 1 32 1 33 1 34 1 35 1 36 1 37 1 38 1 39 1 41 1 42 1 43 1 44 1 45 1 46 1 47 1 48 1 49 1 50 1 51 1 52 1 53 1 54 1 55 1 56 1 57 1 58 1 59 1 60
com pound concentration
control control control control control control control control control PFB S 1 uM PFB S 1 uM PF B S 10 uM PF B S 10 uM PFBS 100 uM PFBS 100 uM P FH S 1 uM P FH S 1 uM PFH S 10 uM PFH S 10 uM P FH S 100 uM P FH S 100 uM PFO S 1 uM PFO S 1 uM P FO S 10 uM P FO S 10 uM P FO S 100 uM P FO S 100 uM R osuvastatin 0.003 uM R osuvastatin 0.003 uM R osuvastatin 0.03 uM R osuvastatin 0.03 uM R osuvastatin 0.3 uM R osuvastatin 0.3 uM PFB S 1 uM PFB S 1 uM PF B S 10 uM PFBS 100 uM PFBS 100 uM P FH S 1 uM P FH S 1 uM PFH S 10 uM PFH S 10 uM P FH S 100 uM P FH S 100 uM PFO S 1 uM PFO S 1 uM P FO S 10 uM P FO S 10 uM P FO S 100 uM P FO S 100 uM R osuvastatin 0.003 uM R osuvastatin 0.003 uM R osuvastatin 0.03 uM R osuvastatin 0.03 uM R osuvastatin 0.3 uM R osuvastatin 0.3 uM
l4C L a b e l
l4C a c e tic a c id 14C a c e tic a c id 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C la u ric a c id 14C la u ric a c id 14C p a lm itic a c id 14C p a lm itic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid 14C b u ty ric acid
3H -c h o le s te ro l (dpm ) 12507 19023 16324 15491 14301 15647 14301 16834 17733 17724 15643 13898 17481 17149 18303 17772 16907 17592 16152 18689 17098 17841 19413 17762 14828 16243 15814 15332 14969 14770 14215 16761 16727 15968 14142 16118 16293 19457 18922 17479 17443 16118 18498 17002 17226 16924 15074 17062 15031 18507 14681 15247 15905 17593 13507 18268
14C -c h o le s te ro l (dpm ) 44451 99078 18935 15714 17937 40817 32511 5000 4789 22246 21849 22496 26287 23557 22881 35195 39844 23683 24871 27769 22118 27624 43559 25533 24114 22967 26323 2953 3029 574 626 257 248 5934 11778 13512 9931 7769 6439 14976 7993 9922 8585 10438 7974 8198 12993 18014 12106 9530 930 687 276 212 94 120
14C -c h o le s te ro l c o rre c te d fo r recovery (dpm ) 91111 133519 29736 26005 32153 66874 58279 7614 6923 32176 35806 41495 38550 35215 32048 50768 60414 34512 39474 38091 33162 39693 57521 36851 41690 36248 42672 4938 5187 996 1129 393 380 9527 21350 21491 15626 10236 8724 21965 11747 15781 11898 15738 11867 12418 22097 27066 20647 13201 1624 1155 445 309 178 168
ug protein
328.5 397.9 305.8 355.1 310.3 399.7 248.7 225.8 204.8 401.4 294.0 493.9 296.9 299.9 228.4 363.1 240.5 320.8 302.8 279.5 394.5 331.5 394.5 441.9 358.3 296.9 376.2 314.8 229.8 351.9 233.8 228.4 330.0 333.1 524.8 464.0 204.8 302.8 279.5 424.0 363.1 282.4 344.0 261.2 406.5 518.7 469.7 344.0 372.9 485.0 394.5 475.3 425.9 475.3 425.9 301.3
14C -c h o le s te ro l per m g protein (dpm /m g)
277384 335520 97240 73233 103632 167329 234314 33725 33809 80168 121789 84018 129826 117430 140298 139810 251169 107571 130352 136259 84052 119725 145791 83394 116357 122069 113436 15686 22576
2831 4829 1721 1152 28602 40681 46312 76307 33802 31206 51807 32351 55879 34586 60261 29192 23940 47045 78680 55370 27218 4116 2430 1044 650 419 559
A v e r a g e '4C - c h o le s te ro l per m g protein (dpm /m g)
306452 91368
200821 33767 100979 106922 128864 195490 118962 110155 132758 99876 117752 19131 3830 1436 34641 46312 55054 41506 44115 47423 26566 62863 41294 3273
847 489
% of control Average SD % of control
90.5
100.0
13.4
109.5
106.4
100.0
17.5
80.2
113.4
83.3
100.0
23.6
116.7
99.9
100.0
0.18
100.1
26.2
33.0
9.6
39.7
27.4
34.9
10.6
42.4
38.3
42.1
5.3
45.8
45.6
63.8
25.7
82.0
35.1
38.8
5.3
42.5
44.5
35.9
12.0
27.4
39.1
43.3
6.0
47.6
27.2
32.6
7.6
38.0
39.8
38.4
2.0
37.0
5.1 6 .2 1.6
7.4
0.9 1.2 0.5
1.6
0 .6 0 .5 0.1
0.4
31.3
37.9
9.3
44.5
50.7
50.7
83.5
60.3
32.9
37.0
34.2
45.4
15.9
56.7
35.4
48.3
18.2
61.2
37.9
51.9
19.9
66.0
32.0
29.1
4.1
26.2
51.5
68.8
24.5
86.1
60.6
45.2
21.8
29.8
4.5 3.6 1.3
2.7
1.1 0 .9 0 .3
0.7
0 .5 0 .5 0.1
0.6
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CD CI /JI
(-D\
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5'C/)"
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Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A167 of A172
3H cholesterol sam ple m ix
1 61 1 62 1 63 1 64 1 65 1 66 1 67 1 68 1 69 1 70 1 71 1 72 2 73 2 74 2 75 2 76 2 77 2 78 2 79 2 80 2 81 2 82 2 83 2 85 2 86 2 87 2 88 2 89 2 90 2 91 2 92 2 93 2 94 2 95 2 96 2 97 2 98 2 99 2 100 2 101 2 102 2 103 2 104 2 105 2 106 2 107 2 108
com pound concentration
PFBS 1 uM PFBS 1 uM PFBS 10 uM PFBS 10 uM PFBS 100 uM PFBS 100 uM PFHS 1 uM PFHS 1 uM PFH S 10 uM PFH S 10 uM PFHS 100 uM PFHS 100 uM PFOS 1 uM PFOS 1 uM PFO S 10 uM PFO S 10 uM PFO S 100 uM PFO S 100 uM Rosuvastatin 0.003 uM Rosuvastatin 0.003 uM Rosuvastatin 0.03 uM Rosuvastatin 0.03 uM Rosuvastatin 0.3 uM PFBS 1 uM PFBS 1 uM PFBS 10 uM PFBS 10 uM PFBS 100 uM PFBS 100 uM PFHS 1 uM PFHS 1 uM PFH S 10 uM PFH S 10 uM PFHS 100 uM PFHS 100 uM PFOS 1 uM PFOS 1 uM PFO S 10 uM PFO S 10 uM PFO S 100 uM PFO S 100 uM Rosuvastatin 0.003 uM Rosuvastatin 0.003 uM Rosuvastatin 0.03 uM Rosuvastatin 0.03 uM Rosuvastatin 0.3 uM Rosuvastatin 0.3 uM
14C Label
3 H -c h o le s te ro l
14C -c h o le s te ro l
(dpm)
(dpm)
l4C lauric acid
18064
35197
14C la u ric a c id
16312
33843
14C la u ric a c id
16632
31024
14C la u ric a c id
16430
42519
14C la u ric a c id
17140
24245
14C la u ric a c id
16929
22983
14C la u ric a c id
17947
34462
14C la u ric a c id
17680
35488
14C la u ric a c id
17511
31561
14C la u ric a c id
18652
34500
14C la u ric a c id
17842
20913
14C la u ric a c id
17390
30612
14C la u ric a c id
12194
39952
14C la u ric a c id
17125
49945
14C la u ric a c id
16554
29563
14C la u ric a c id
16019
42312
14C la u ric a c id
15880
22742
14C la u ric a c id
17209
31390
14C la u ric a c id
15981
3218
14C la u ric a c id
14109
3171
14C la u ric a c id
17448
754
14C la u ric a c id
16191
686
14C la u ric a c id
15558
261
14C p a lm itic a c id
16555
4312
14C p a lm itic a c id
17040
3219
14C p a lm itic a c id
15827
3366
14C p a lm itic a c id
16659
4602
14C p a lm itic a c id
15285
2953
14C p a lm itic a c id
17337
3284
14C p a lm itic a c id
14572
2399
14C p a lm itic a c id
14708
3322
14C p a lm itic a c id
15562
3238
14C p a lm itic a c id
14849
3857
14C p a lm itic a c id
16477
2952
14C p a lm itic a c id
15761
3697
14C p a lm itic a c id
16923
3859
14C p a lm itic a c id
19055
4608
14C p a lm itic a c id
16210
4074
14C p a lm itic a c id
17729
5568
14C p a lm itic a c id
16090
2981
14C p a lm itic a c id
17458
3072
14C p a lm itic a c id
12554
711
14C p a lm itic a c id
17205
986
14C p a lm itic a c id
16635
534
14C p a lm itic a c id
17350
544
14C p a lm itic a c id
13361
313
14C p a lm itic a c id
15847 m ix 1
359 m ix 2
input 3H cholesterol
25860
24418
input 3H cholesterol input 3H cholesterol
25671 25376
25195 24950
average input 3H cholesterol
2 5 6 3 5 .7
2 4 8 5 4 .3
C -cholesterol corrected fo r recovery (dpm) 49950 53187 47819 66342 36262 34803 49226 51457 46205 47417 30048 45127 81432 72488 44386 65649 35594 45335 5005 5586 1074 1053 417 6474 4695 5286 6866 4802 4708 4092 5614 5171 6456 4453 5830 5668 6010 6247 7806 4605 4373 1408 1424 798 779 582 563
ug protein
3 2 1 .4 4 4 4 .2 3 3 5 .0 396.1 3 1 0 .4 3 5 7 .5 3 9 2 .2 297.1 3 9 3 .5 3 2 6 .3 3 1 6 .5 2 7 6 .8 4 3 2 .0 3 3 7 .5 3 2 1 .4 3 7 7 .9 3 5 6 .3 3 6 7 .7 4 6 3 .5 2 8 8 .7 4 9 1 .7 2 9 2 .0 4 3 8 .8 3 8 0 .5 4 6 4 .4 4 1 7 .2 2 8 3 .9 3 8 4 .4 3 7 5 .4 4 0 8 .0 3 2 7 .5 3 6 6 .4 3 8 5 .7 3 3 8 .7 3 0 8 .0 3 8 4 .4 4 4 2 .9 3 9 2 .2 2 9 2 .3 4 4 7 .0 3 3 6 .2 396.1 3 3 0 .0 374.1 3 3 3 .7 3 9 4 .8 2 8 9 .9
14C -c h o le s te ro l per m g protein (dpm /m g)
155414 119730 142754 167480 116825 97343 125512 173201 117417 145310 94937 163023 188500 214808 138103 173700 99909 123297 10797 19348
2185 3606 950 17013 10110 12669 24182 12492 12542 10030 17139 14114 16738 13147 18930 14744 13572 15927 26705 10302 13008 3554 4316 2133 2335 1475 1942
A v e ra g e 14C -c h o le s te ro l per m g protein (dpm /m g)
137572 155117 107084 168120 131364 128980 201654 155901 111603 15073
2895 1050 13562 18425 42805 13584 15426 16038 14158 21316 11655 3935 2234 8190
% of control Average SD % of control
77.4
68.5
12.6
59.6
71.1
77.2
8.7
83.4
58.2
53.3
6.9
48.5
62.5
74.4
16.8
86.2
58.5
65.4
9.8
72.4
47.3
64.2
24.0
81.2
93.9
100.4
9.3
107.0
68.8
77.6
12.5
86.5
49.8
55.6
8.2
61.4
5.4 7.5 3.0
9.6
1.1 1.4 0.5
1.8
0.5 0.5 0.1
50.4
50.4
37.5 71.6 37.0 37.1 29.7 50.8 41.8 49.6 38.9 56.1 43.7 40.2 47.2 79.1 30.5 38.5 10.5 12.8 6.3 6.9 4.4 5.8
54.6 37.1 40.2 45.7 47.5 41.9 63.1 34.5 11.7 6.6 5.1
24.1 0.1 14.9 5.5 12.1 2.5 22.6 5.7 1.6 0.4 1.0
Appendix to TNO-report of 3M-02 Experiment 4
Final version
04-12-2006
Protein concentration determination
sample
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56
Measured protein conc (mg/ml)
well 1
well 2
207.81
223.77
214.31
224.72
189.73
202.31
205.97
230.5
234.4
252.34
221.86
205.97
218.07
224.72
225.68
225.68
214.31
223.77
210.58
214.31
210.58
220.91
199.58
205.97
212.44
214.31
241.29
245.28
230.5
226.54
234.4
239.31
272.05
262.61
292.63
272.24
254.37
242.28
231.47
237.34
269.94
274.18
272.05
253.35
253.35
250.31
242.28
221.86
202.31
210.58
241.29
242.28
245.28
239.31
230.5
235.37
245.28
233.42
218.07
209.66
250.31
240.3
253.35
242.28
244.28
249.3
233.42
237.34
215.24
204.14
227.6
210.58
249.52
228.83
256.56
246.52
277.1
284.44
248.52
238.6
251.52
254.54
266.75
258.59
238.6
246.52
265.73
254.54
251.52
253.53
242.55
245.53
237.62
229.8
212.56
208.8
211.62
203.19
206.92
209.73
194.87
192.13
206.92
210.68
222.08
234.67
177.7
186.68
214.45
228.83
220.17
214.45
dilution
5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5
protein conc
(mg/ml)
1079.0 1097.6 980.1 1091.2 1216.9 1069.6 1107.0 1128.4 1095.2 1062.2 1078.7 1013.9 1066.9 1216.4 1142.6 1184.3 1336.7 1412.2 1241.6 1172.0 1360.3 1313.5 1259.2 1160.4 1032.2 1208.9 1211.5 1164.7 1196.8 1069.3 1226.5 1239.1 1234.0 1176.9 1048.5 1095.5 1195.9 1257.7 1403.9 1217.8 1265.2 1313.4 1212.8 1300.7 1262.6 1220.2 1168.6 1053.4 1037.0 1041.6 967.5 1044.0 1141.9 911.0 1108.2 1086.6
pg protein
per well
359.7 365.9 326.7 363.7 405.6 356.5 369.0 376.1 365.1 354.1 359.6 338.0 355.6 405.5 380.9 394.8 445.6 470.7 413.9 390.7 453.4 437.8 419.7 386.8 344.1 403.0 403.8 388.2 398.9 356.4 408.8 413.0 411.3 392.3 349.5 365.2 398.6 419.2 468.0 405.9 421.7 437.8 404.3 433.6 420.9 406.7 389.5 351.1 345.7 347.2 322.5 348.0 380.6 303.7 369.4 362.2
Page A168 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
sam ple
57 58 59 60 61
62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 91 92 94 95 96 97 98 100 101 103 104 105 106 107 109 110 112
113
114 115 116 118 119
M easured protein conc (m g/m l)
well 1
well 2
224.96 219.21 213.51 199.48 210.68
223.04 218.26 204.12 205.99 204.12
186.68 160.17 209.73 205.99 229.8 237.62 222.08
193.04 161.9 203.19 217.3 225.93 246.52 234.67
261.64 259.6 239.59 278.14 212.61 232.92 225.79 231.13 233.82 225.79 241.03 239.22 217.85 224.02 220.49
283
263.68 235.65 255.55 285.49 215.22 229.35 240.13 233.82 241.03 239.22 238.32 232.03 221.37 234.72 225.79 287.81
208.27 204.81 203.09 218.73 216.10 205.67 224.02 218.73 233.82 226.68 242.85 252.91 242.85 218.73 255.68 242.85 248.32 229.35 251.99
190.32 214.35 217.85 214.35 212.61 203.95 217.85 211.74 224.02 218.73 235.62 239.22 245.58 209.13 256.60 245.58 237.42 242.85 243.76
245.58 231.13 251.07
259.39 217.85 255.68
272.51
291.69
219.61 243.74 255.52 267.47 234.05
216.10 246.67 269.48 271.50 235.01
d ilu tio n
5 5 5 5 5
5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5
5
5 5 5 5 5
protein conc
(m g/m l) 1120.0 1093.7 1044.1 1013.7 1037.0
949.3 805.2 1032.3 1058.2 1139.3 1210.4 1141.9 1313.3 1238.1 1237.9 1409.1 1069.6 1155.7 1164.8 1162.4 1187.1 1162.5 1198.4 1178.1 1098.1 1146.9 1115.7 1427.0 996.5 1047.9 1052.4 1082.7 1071.8 1024.1 1104.7 1076.2 1144.6 1113.5 1196.2 1230.3 1221.1 1069.7 1280.7 1221.1 1214.4 1180.5 1239.4 1262.4 1122.5 1266.9
1410.5
1089.3 1226.0 1312.5 1347.4 1172.7
|jg protein
per w ell 373.3 364.6 348.0 337.9 345.7
316.4 268.4 344.1 352.7 379.8 403.5 380.6 437.8 412.7 412.6 469.7 356.5 385.2 388.3 387.5 395.7 387.5 399.5 392.7 366.0 382.3 371.9 475.7 332.2 349.3 350.8 360.9 357.3 341.4 368.2 358.7 381.5 371.2 398.7 410.1 407.0 356.6 426.9 407.0 404.8 393.5 413.1 420.8 374.2 422.3
470.2
363.1 408.7 437.5 449.1 390.9
Page A169 of A172
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A170 of A172
3H cholesterol sam ple m ix 11 12 13 14 15 17 18 19 1 10 1 11 1 12 1 13 1 15 1 17 1 18 1 19 1 20 1 22 1 23 1 24 1 25 1 26 1 27 1 28 1 30 1 31 1 32 1 33 1 34 1 35 1 36 1 38 1 39 2 40 2 41 2 42 2 43 2 44 2 45 2 46 2 47 2 48 2 49 2 50 2 51 2 52 2 53 2 54 2 56 2 57 2 58
com poun d concentration
control control control control control control control control control control control P F B S 1 |j M PFBS 1 j M P FB S 10 j M P FB S 10 j M PFBS 100j M PFBS 100j M PFHS 1 j M PFHS 1 j M PFHS 1 j M PFHS 10 j M PFHS 10 j M PFHS 10 j M PFHS 1 00j M PFHS 1 00j M PFOS 1 j M PFOS 1 j M PFOS 1 j M PFO S 10 j M PFO S 10 j M PFO S 10 j M PFO S 100 j M PFO S 100 j M R osuvastatin 0.003 j M R osuvastatin 0.003 j M R osuvastatin 0.003 j M R osuvastatin 0.03 j M R osuvastatin 0.03 j M R osuvastatin 0.03 j M R osuvastatin 0.3 j M R osuvastatin 0.3 j M R osuvastatin 0.3 j M PFBS 1 j M PFBS 1 j M PFBS 1 j M P FB S 10 j M P FB S 10 j M P FB S 10 j M PFBS 100j M PFBS 100j M PFHS 1 j M
14C L a b e l
l4C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C p a lm itic a cid 14C p a lm itic a cid 14C p a lm itic a cid 14C p a lm itic a cid 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id
14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id 14C a c e tic a c id
14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id 14C b u ty ric a c id
3H -c h o le s te ro l 14C -c h o le s te ro l
(dpm )
(dpm )
11557 13921
1815 1877
12789 14330
2094 2430
14143
2257
12409
2888
13395
4007
12206
1142
13316
1694
12616
1574
13307 14019
1703 2569
14018 13123
1506 1714
12859 12350
1837 1427
12583
2709
12002
1811
12074
1822
10469
2089
12178 13070
2935 3091
11021 12494
3398 2059
11696 12079
1924 3650
12015
2929
12687
2772
12477
2383
12560
2407
10462 10416
1828 1647
9390
1807
9534
81
11731
95
12187
61
11494
36
12998
42
10852
18
9480
7
8849 11450
10 12
9541 11879
1216 2158
12114 11350
3192 998
11456
1499
9935
1125
11036 9818
4223 1517
12175
1686
14C -c h o le s te ro l c o rre c te d for recovery (dpm ) 3220 2765 3357 3477 3272 4772 6134 1918 2608 2558 2624 3757 2203 2678 2929 2369 4414 3094 3094 4091 4942 4849 6322 3379 3373 6196 4998 4480 3916 3929 3583 3242 3946 171 163 100 63 65 33 15 23 21 2559 3647 5290 1765 2627 2273 7682 3102 2780
jg protein
3 5 9 .7 3 6 5 .9 3 2 6 .7 3 6 3 .7 4 0 5 .6 3 6 9 .0 376.1 365.1 354.1 3 5 9 .6 3 3 8 .0 3 5 5 .6 3 8 0 .9 4 4 5 .6 4 7 0 .7 4 1 3 .9 3 9 0 .7 4 3 7 .8 4 1 9 .7 3 8 6 .8 344.1 4 0 3 .0 4 0 3 .8 3 8 8 .2 3 5 6 .4 4 0 8 .8 4 1 3 .0 4 1 1 .3 3 9 2 .3 3 4 9 .5 3 6 5 .2 4 1 9 .2 4 6 8 .0 4 0 5 .9 4 2 1 .7 4 3 7 .8 4 0 4 .3 4 3 3 .6 4 2 0 .9 4 0 6 .7 3 8 9 .5 351.1 3 4 5 .7 3 4 7 .2 3 2 2 .5 3 4 8 .0 3 8 0 .6 3 0 3 .7 3 6 2 .2 3 7 3 .3 3 6 4 .6
14C -c h o le s te ro l per m g protein (dpm /m g)
8953 7556 10276 9559 8067 12932 16307 5255 7367 7114 7764 10566 5784 6011 6223 5724 11299 7066 7372 10578 14362 12033 15655 8704 9463 15155 12102 10892 9982 11243 9811 7733 8432 420 386 230 156 150
79 36 58 60 7402 10504 16403 5073 6902 7487 21211 8309 7626
% o f control Average % o f control
9 8 .5
1 0 0 .0
83.2
113.1
105.2
6 4 .9
1 0 0 .0
1 0 4 .0
131.1
7 6 .4
1 0 0 .0
107.2
1 0 3 .5
1 1 2 .9
1 1 6 .3
90.0
63.7
66.2
67.3
6 8 .5
6 3 .0
93.7
1 2 4 .4
7 7 .8
91.8
81.1
1 1 6 .4
158.1
1 5 4 .3
1 3 2 .4
1 7 2 .3
9 5 .8
1 0 0 .0
104.1
1 6 6 .8
1 3 9 .9
133.2
1 1 9 .9
1 0 9 .9
1 1 3 .9
123.7
1 0 8 .0
85.1
89.0
9 2 .8
4.6 3.8
4.2
2.5
1.7 1.4
1.6
0.9
0.4 0.6
0.6
0.7
5 9 .5
92.0
8 4 .5
1 3 1 .9
4 0 .8
52.2
5 5 .5
60.2
1 7 0 .6
1 1 8 .7
6 6 .8
6 1 .3
1 2 4 .5
SD 12.7
3 3 .3 16.2
37.2 1.6 4 3 .4 2 1 .4 20.2 5.9 24.2 8.6 5.4 1.1 0.5 0.1 3 6 .8 10.1 7 3 .4 62.2
0 1 o
CD CI /JI
(-D\
O C/) r3+ 3" CD C/)
5'C/)"
-\
0)
CD "D
0io)i
0 V1CI CD C/)
Appendix to TNO-report of 3M-02____________ Final version__________________________04-12-2006
Page A171 of A172
3H cholesterol s a m p le m ix
2 59 2 60 2 61 2 62 2 64 2 66
2 67 2 68 2 69 2 70 2 71 2 72 2 73 2 74 2 75 2 76 2 77 2 78 2 79 2 80 2 81 2 82 2 83 2 85 2 86 2 87 2 88 2 89 2 91 2 92 2 94 2 95 2 96 2 97 2 98 2 100 2 101 2 103 2 104 2 105 2 106 2 107 2 109 2 110 2 112 2 113 2 114 2 115 2 116 2 118 2 119
com p o u n d co n ce n tra tio n
PFH S 1 pM PFH S 1 pM P F H S 1 0 pM P F H S 1 0 pM PFH S 1 0 0 pM PFH S 1 0 0 pM P F O S 1 pM P F O S 1 pM PFO S 1 pM PFO S 10 pM PFO S 10 pM PFO S 10 pM PFOS 1 0 0 pM PFOS 1 0 0 pM PFOS 1 0 0 pM R osu va sta tin 0.003 pM R osu va sta tin 0.003 pM R osu va sta tin 0.003 pM R osu va sta tin 0.0 3 pM R osu va sta tin 0.0 3 pM R osu va sta tin 0.0 3 pM R o suvastatin 0.3 pM R o suvastatin 0.3 pM P FBS 1 pM P FBS 1 pM P FBS 1 pM P FB S 10 pM P FB S 10 pM PFBS 100 pM PFBS 100 pM PFH S 1 pM PFH S 1 pM PFH S 1 pM PFH S 10 pM PFH S 10 pM PFH S 1 0 0 pM PFH S 1 0 0 pM PFO S 1 pM PFO S 1 pM PFO S 1 pM PFO S 10 pM PFO S 10 pM PFOS 1 0 0 pM PFOS 1 0 0 pM R osu va sta tin 0.003 pM R osu va sta tin 0.003 pM R osu va sta tin 0.003 pM R osu va sta tin 0.0 3 pM R osu va sta tin 0.0 3 pM R o suvastatin 0.3 pM R o suvastatin 0.3 pM
14C L a b e l
3H -cholesterol (dpm )
l4C b u ty ric a cid
11671
l4C b u ty ric a cid
11805
14C b u ty r ic a c id
11641
14C b u ty r ic a c id
11648
14C b u ty r ic a c id 14C b u ty r ic a c id
11906 12251
14C b u ty r ic a c id
12599
14C b u ty r ic a c id
10509
14C b u ty r ic a c id
12593
14C b u ty r ic a c id
12245
14C b u ty r ic a c id
11423
14C b u ty r ic a c id
12013
14C b u ty r ic a c id
11885
14C b u ty r ic a c id 14C b u ty r ic a c id
11791 11061
14C b u ty r ic a c id
12385
14C b u ty r ic a c id
12579
14C b u ty r ic a c id
12107
14C b u ty r ic a c id
12774
14C b u ty r ic a c id
11196
14C b u ty r ic a c id
12355
14C b u ty r ic a c id
13007
14C b u ty r ic a c id
12240
14C p a lm itic a c id
14199
14C p a lm itic a c id
12961
14C p a lm itic a c id
13307
14C p a lm itic a c id
13107
14C p a lm itic a c id
12361
14C p a lm itic a c id
14351
14C p a lm itic a c id
12742
14C p a lm itic a c id
13653
14C p a lm itic a c id
12703
14C p a lm itic a c id
12038
14C p a lm itic a c id
13137
14C p a lm itic a c id
11960
14C p a lm itic a c id
11398
14C p a lm itic a c id
14337
14C p a lm itic a c id
11689
14C p a lm itic a c id
13496
14C p a lm itic a c id
13069
14C p a lm itic a c id
13862
14C p a lm itic a c id
14202
14C p a lm itic a c id
9771
14C p a lm itic a c id
8624
14C p a lm itic a c id
6247
14C p a lm itic a c id
8729
14C p a lm itic a c id
10667
14C p a lm itic a c id
6797
14C p a lm itic a c id
4697
14C p a lm itic a c id
9054
14C p a lm itic a c id
4674 m ix 1
input 3H cholesterol
21387
input 3H cholesterol input 3H cholesterol
20596 19529
a v e ra g e in p u t 3H c h o le s te ro l
20504
C -ch o le ste ro l (dpm ) 3180 4589 3479 4837 2793 3204 1926 2215 2141 2079 2076 2892 2655 2641 2162 137 127 162 35 33 43 30 43 978 745 963 863 429 1098 954 1536 1392 1184 1031 991 1551 1171 851 1062 1193 1377 1377 742 980 152 122 128 127 107 141 125 m ix 2 21723 17423 21082 20076
14C - c h o le s te r o l c o r r e c te d fo r recovery (dpm ) 5470 7804 6000 8337 4710 5250 3069 4231 3413 3409 3649 4833 4485 4497 3924 222 203 269 55 59 70 46 71 1383 1154 1453 1322 697 1536 1503 2259 2200 1975 1576 1663 2732 1640 1462 1580 1833 1994 1947 1525 2281 488 281 241 375 457 313 537
pg protein
3 4 8 .0 3 3 7 .9 3 4 5 .7 3 1 6 .4 344.1 3 7 9 .8 4 0 3 .5 3 8 0 .6 4 3 7 .8 4 1 2 .7 4 1 2 .6 4 6 9 .7 3 5 6 .5 3 8 5 .2 3 8 8 .3 3 8 7 .5 3 9 5 .7 3 8 7 .5 3 9 9 .5 3 9 2 .7 3 6 6 .0 3 8 2 .3 3 7 1 .9 3 3 2 .2 3 4 9 .3 3 5 0 .8 3 6 0 .9 3 5 7 .3 3 4 1 .4 3 6 8 .2 3 5 8 .7 3 8 1 .5 3 7 1 .2 3 9 8 .7 410.1 4 0 7 .0 3 5 6 .6 4 2 6 .9 4 0 7 .0 4 0 4 .8 3 9 3 .5 413.1 4 2 0 .8 3 7 4 .2 4 2 2 .3 4 7 0 .2 363.1 4 0 8 .7 4 3 7 .5 449.1 3 9 0 .9
14C - c h o le s te r o l per m g protein (dpm /m g)
15718 23097 17357 26346 13687 13825 7607 11117 7797 8259 8843 10290 12579 11673 10107
573 512 693 138 151 191 121 190 4163 3304 4142 3663 1950 4500 4082 6296 5766 5320 3952 4056 6712 4599 3424 3881 4527 5068 4712 3623 6097 1157 597 663 918 1045 696 1374
% o f control A verage % of control
1 2 6 .4
1 8 5 .7
1 3 9 .6
1 7 5 .7
2 1 1 .9 110.1 111.2
110.6
6 1 .2 8 9 .4
71.1
6 2 .7
6 6 .4 71.1
7 3 .4
8 2 .7 101.2
92.1
9 3 .9 8 1 .3
4.6 4.8
4.1 5.6 1.1 1 .3 1.2
1.5 1.0 1.2
1.5
6 0 .6 48.1
5 6 .3
6 0 .2
5 3 .3
4 0 .8
2 8 .4
6 5 .5
6 2 .4
5 9 .4
9 1 .6
8 4 .3
8 3 .9 7 7 .4
5 7 .5
5 8 .2
5 9 .0
9 7 .6 6 6 .9
8 2 .3
4 9 .8
5 7 .4
5 6 .5 6 5 .9
7 3 .7
71.1
6 8 .5
5 2 .7 8 8 .7
7 0 .7
16.8
11.7
8.7
9.7
13.4
14.3
15.2 10.1 20.0
15.1
SD
6 4 .8 0.8 15.9
8.4 10.1
0.7 0.2
0.4 7.1
17.6 4.3 7.1 1.1 2 1 .7 8.1
3.7 2 5 .5 4.4
1.3 7.0
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Average [14C]-Cholesterol synthesis in rat hepatocytes (% of control)
C oncentration: 1 mM 1 10 m M | 100 m M 1 0.003 m M 0.03 uM
experim ent 1 2 3 4
l4C a ce ta te in co rp o ra tio n , n=4, PFBS
85.0
82
76
115 157
177
33 34.9
42.1
90 67.3
93.7
AVERAGE SD
80.8 34.4
85.3 51.7
97.2 57.3
experim ent 1 2 3 4
AVERAGE SD
14C a ce ta te in co rp o ra tio n , n=4, PFH S
91 82
84
148 136
111
63.8 91.8
38.8 154.3
35.9 100
98.7 102.8
82.7
35.4
52.5
33.1
experim ent 1
14C a ce ta te in co rp o ra tio n , n=4, PFO S
76 84
65
2
130 119
132
3
43.3
32.6
38.4
4
139.9 113.9
89
AVERAGE SD
97.3 45.7
87.4 39.6
81.1 39.7
experim ent 1 2 3 4
14C a ce ta te in co rp o ra tio n , n=4, R osuvastatin
45 40.0 6.2 3.8
11 8 1 1
AVERAGE SD
23.8 21.8
5.4 4.9
0.3 uM
3 2 0.5 0.6 1.5 1.2
C o n c e n tra tio n : 1 u M | 10 imM I 100 u M | 0 .0 0 3 uM
experim ent 1 2 3 4
14C b u ty ra te in c o rp o ra tio n , n= 4, P FB S
61.0
128
92
24
146 140
175
37.9
50.7
60.3
92 52.2
118.7
AVERAGE SD
84.2 46.8
92.7 47.9
111.5 48.6
experim ent 1 2 3 4
14C b u ty ra te in c o rp o ra tio n , n= 4, PFH S
115 101
100
190 220
200
45.4
48.3
51.9
124.5 175.7
110.6
AVERAGE SD
118.7 59.2
136.3 76.5
115.6 61.8
experim ent 1 2 3 4
14C b u ty ra te in c o rp o ra tio n , n= 4, P F O S
76 98
58
262 223
286
29.1 68.8
45.5
71.1
73.4
92.1
AVERAGE SD
109.6 103.8
115.8 72.6
120.4 112.1
experim ent 1 2 3 4
14C b u ty ra te in c o rp o ra tio n , n= 4, ro s u va sta tin
24 46.0 3.7 4.8
AVERAGE SD
19.6 19.9
0.03 uM 5
5 16 1 1 5.8 7.0
0.3 uM 3.0
3 4 0.5 1.2 2.2 1.6
C oncentration: 1 uM | 10 uM | 100 uM | 0.003 uM
experim ent 1 2 3
14C la u ra te in co rp o ra tio n , n= 3, P F B S
84.0
97
87
131 102
100
68.5
77.2
53.3
AVERAGE SD
94.5 32.5
92.1 13.1
80.1 24.1
1 2 3
AVERAGE SD
14C la u ra te in co rp o ra tio n , n= 3, P F H S
108 102
68
150 103
134
74.4
65.4
64.2
110.8 90.1
88.7
37.9
21.4
39.2
1 2 3
AVERAGE SD
14C la u ra te in co rp o ra tio n , n= 3, P F O S
113 85
65
119 124
125
100.4
77.6
55.6
110.8 95.5
81.9
9.5 24.9
37.6
14C la u ra te in co rp o ra tio n , n= 3, ro s u v a s ta tin
1 25 2 35.0 3 7.5
AVERAGE SD
22.5 13.9
0.03 uM
7 15 1 7.8 6.8
0.3 uM
3 3 0.5 2.2 1.4
C oncentration: 1 uM
10 uM
100 uM
0.003 uM 0.03 uM
experim ent 1 3 4
14C p a lm ita te in co r poration, n=3, P FB S
76.0
56
71
50.4
54.6
37.1
56.3
40.8
62.4
AVERAGE SD
60.9 13.4
50.5 8.4
56.8 17.6
CO X LL Q_ CO II c c o
o
14C p a lm ita te in co r
1
63 68
59
3
40.2
45.7
47.5
4
84.3
58.2
82.3
AVERAGE SD
62.5 22.1
57.3 11.2
62.9 17.7
14C p a lm ita te in co r poration, n=3, P F O S
1
90 62
51
3
41.6
63.1
34.5
4
57.4
71.1
70.7
AVERAGE SD
63.0 24.7
65.4 5.0
52.1 18.1
14C p a lm ita te in co r poration, n=3, ro s u v a s ta tin
1 48 20
3
11.7
7
4
11.7
14
AVERAGE SD
23.8 21.0
13.8 6.5
0.3 uM
12 5 15.1 10.7 5.2
Page A172 of A172
