Document zQYogy6mYqXn547bvaDg73Gag

ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referretdoas PFOS, FC-95,or as a component ofFC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productiolnotnumber was notnoted.The testsample isFC-203. Currentinformatioinndicateistisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol2,.66 % Sultonefoamer,3% sodium octylsulfate0,.1% sodium lauryslulfatea,nd 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrationsof impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: Fishbioassayprocedures,StandardMethods, 1970 edition. Type: Flow-ThroughAcute GLP: No Year completed: 1972 Species: Lepomis macrochirus Supplier:CommercialhatcheryinNebraska. Analyticalmonitoring:Temperature,pH,conductivitayn,d DO Exposure period:24,96,and 264 hours. Statisticamlethods: TL50(incipiemnetdiantolerancleimitv)alues calculatedusinga linearregressionequation. Test fishage: Juveniles Length and weight: Average length 56 mm Average weight 2.4g (wet) Loading: Not given Pretreatment:None Testconditions: Dilutiownater:Aeratedwellwater Dilutiownaterchemistry: Hardness: 38 mg/L as CaC03 Lighting:Notgiven. Stockand testsolutionpreparationS:ufficieanmtountsofstocksolution containingthecompound initialaldyded toeach testvessel.Proportional diluteursed to maintaindesiredconcentrations. Flow-through rate:Continuousat5 L everyhour. Stabilitoyf the testchemical solutions:Not noted Exposure vessels: 30 L Number of replicates:1 Number offishperreplicate3:0 Number ofconcentrationss:evenplusa blankcontrol Water chemistryduringthestudy: pH range(0-96hours): 7.1- 7.1(contreoxlposure) 6.8- 6.9(33.4mg/L exposure) Temperature range (0-96hours): 18 1 *C Dissolved oxygen range (0-96hours): 9.1-9.3mg/L RESULTS Nominal concentrations: Blank control5,.9,7.9,10.5,14.1,18.8,25.0,and 33.4 mg/L Element values: 96-hour TL50 = 20.4 (16.4- 25.3)mg/L 264-hourTL50 = 15.9(12.5- 20.2)mg/L 264-hour NOEC = 5.9 mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-203 96-hour TL50 forfatheadminnow was determined tobe 20.4 mg/L witha 95% confidenceintervaolf 16.4to 25.3 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking= 3. Testingwas done usinglaboratory procedures notconsistentwithcurrentregulatoryapproaches. The sample puritywas not properlycharacterizedand the study lacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES Test was conducted by Bionomics,Inc.,ofWareham, MA atthe requestof the 3M Company, St.Paul,MN, 1972. OTHER Last changed: 6/27/00 7 @.t -vt lg w 7g, I ,TW METHODS AND MATERIALS The investigation was performed at the aquatic toxicology laboratory of Bionomics, Inc., Wareha.m, Massa.chusetts. The susceptibility of bluegill (Lepomis macrochirus) to Light Wate@R aqueous f-*,-lfmorming foam (tested as 100% activ'e) under dynamic conditions was reported as the incipient median tolerance limit (TL the concentration of the 50)' test compound in water causing 50 percent mortality with no additional significant response (>10%) during the final .48 hours of exposure. The predicted TL value and its 50 95% confidence intervals were arrived at by converting the concentra.tions tested. and the corresponding observed percent mortalities to logs a.ndprobits, respectively- These values were then used to calculate a. linear regression equation. Test procedures for the dynamic bioassay are those described for fish Bioassay Procedures in the 1970 edition of Sta.ndard Methods (APHA)s The bluegill were obtained from a commercial fish hatchery in Nebra.sks and had a.mean weight of 2.4 g and a mean length of 56 mm. The dynamic bioa,ssay was conducted using a continuous-flow dilution appa-rattis(Mount and Brungs, 1967) 1 proportional 1 Mount, D. I. and W. A. Brungs. 1967, A simplified dosing a.ppa.ratus for fish toxicology studies. Water Research. 1:21. Page two The apparatus provides for intermittent introduction of seven concentra.tions of the test compound into test vessels and diluent water to a.vessel serving a-s a control unit. Flow rate to each of the 30-liter test vessels was 5 laolir throughout the test period. The test diluent consisted of a..era.tewdell water of PH 7.1, total hardness 38 mg/l as Ca.Co and a c'onsta.nt 3# temperature of 18C (+ 1.0). Dissolved oxygen levels for the test ranged from 9.1 to 9-3 mg,/l. Thirty specimens .were introduced 48 hours prior to the start of the assay into each test unit. The desired concentrations of the test compound were established after the 48 hour a.cclima.tion period in the test vessels by adding sufficient amounts of stock solution conta.ining the compound to each test vessel. The proportional dilution apparatus wa.s then used to maintain.the desired concentration of the compound in each'test vessel. RESULTS The predicted TL 50 values and 95% confidence intervals are presented in Ta.ble 1. The,.data for p,p' DDT, determined at Bionomics by a static bioas say to serve a.sa "standard" indica.te that the population.of test animals is. representa,tive of an "average population". Table 2 presents a 'summary of observed mortality for bluegill after 24 and 96 hours Page three of exposure and end of test. Moribund fish genera.lly beca.me dark and lethargic, lost equilibrium, a.nd expired. Table 3 presents the pH of selected concentra.tions from the test at 24 and 96 hours and-end-of test. SUBMITTED BY: Bionomics, Inc. 790 Main Street Wareha.m, Massachusetts September, 1972 PREPARED BY: APPROVED BY: Bevier Ha.sbrouck sleigh t, WWI Chief, Acute Toxicity and Residue Investigations Kenneth J. Ma.cek, Ph.D. Director Table 1 Acute toxicity of Light Waterq)to bluegilla. (Lepomis macrochirus). The data are-ba.sed on dynalmic bioassays conducted at the Fish Toxicology La.boratory of Bionomics, Inc. in Wareham, Ma.ssachusetts. Compound 24 hour TL 50 mg a-cti-ve iini-g-redient/liter 96 hour Incipient No Eff Level (mg,/l) Light Watero DDT >33.4 20.4(16.4-25-3)c 15-9(12-5-20.2) 5.9 o.ooB(o.oo4-0.012) Assay*conducted at 18C (t 1.0) mea.n weight of bluegill 2.4 g.' b Incipient TL estimated over 264 hours. 50 c 95% cofidence intervals. Ta.ble 2 Concentrations tested a.nd corresponding observed percent mortalities for bluegill (Lepomis ma.crochirus) exposed to Light Watex';Ea'fJter 24 and 96 hours a.nd end of test. Concentration (Mgl/l) 24 hour % mortality observed gb hour Incipient 33.4 25.0 18.8 14.1 10-5 7.9 5-9 Control 264 hours 0 100 100 0 30 64 0 1? 50 0 10 20 0 .0 17 0 0 3 0 0 0 ..o .0 0 4r Table 3 PH of selected concentrations from a.dynamic bioassay conducted with bluegill (Lepomis ma.crochirus) exposed to Light Wateloafter 24 and 96 hours and end of test. Concentration (mg//l) 24 h -u-r- 7 PH hour I n c Tp-'el-n t -33 4 14.1 5 .'9 Control 6. 7 4 7.1, 6- 8:,,-- 7 7. @l -"26@.4@6ur@s 9: 'T4 7., 4