Document zQYN1E95KwJE2xJ4noVw39VK7

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, or FC-109-X. (Glycine, N-ethyl-N[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991 51-7) Remarks: The test sample is FC-127 (F-6390, Lot 1). Current information indicates it is a mixture of 40-44% test substance, 32% water, 14% isopropanol, 4% ethanol, and 6-10% C4-C7 fluoroalkyl carboxylate compounds. The following summary is abbreviated because the test sample is a mixture of the test substance in a water/organic solvent solution. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD: Methods: EPA protocol. Test type: Static acute GLP: No Year Completed: 1981 Species: Pimephales promelas Analytical monitoring: Temperature, pH, DO, and conductivity RESULTS Nominal concentrations: Bk control, 650, 870, 1150,1550, 2100 mg/L Element value: 96-hour LC50 = 863 (796-950) mg/L Element value based on nominal concentrations. Remarks: There was a slight separation in test concentrations after 24 hours (surface film formation).Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The value reported applies to that mixture and not the test substance. No attempt was made to determine the impact of the presence of the organic solvents or what portion of the toxicity can be contributed to the Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS 0005 The FC-127 96-hour LC50 for Pimephales promelas was determined to be 863 mg/L with a 95% confidence interval of 796-950 mg/L by moving average angle method. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted at 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 7009. OTHER Last changed: 5/18/00 000226 BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDING TEST) ivi T .t^ y / - . //'ifT F ^ SrrtT tf'. w trti t t LR / ? . d u ,i_L T .s t O nO TU . f c t f T j t f ^ MlUMOUka u re . K lig T Z ..'^ , S l jg C tfr t . K . C ^ g / S / 6atch H o ^ Avg. Wt. n . Z C K g ra n Avg. S ix e g , Q c M No. O rgatisns por dose 5_____________ Test S olutio n Volt-- 3 L ite r s /A ll-G lo ii T alk T alk Diaaifiais:W IOE-MOUTN I GALLON GLASS J r i s m trt s t . r t . j ^ /A Q J S !____ " c o ^ i . t m y / ^ / / S i_____ Dilution Notor CotDea-fllterod noil w to r (To-- . D.O. \ 0 . f ) ppm. pH ^ .Z 1 Investigator (P u ll S ig u tm LC^ (S Confidale* Linits) Ik irn y fiB ti ia u lF A < ^ n s .c ~ T ii< rr \5 . -ft C L P P i^ . tank NO. Svmtmm H aw tiaw Control' A-J 1 w a 3 js r~ iy k . A \ v / rsinTVjf^O r ^ l k i ncf ^ -U tL A o 9 W C . IM lI No. A IIn MA - i r . _______ tt-M r.___ 4 - 2 x , ____ ! M ir, Zj LNVIKUNMLNTAL LABORATORY ST CUrT AVAILABLE% AT n n n u AQUATIC T O X IC IT Y DATA SHEET D E F IN IT IV E (FULL-SCALE TEST) LR Type Test U T C < srrtD C M a te r ia l T e s t a d ^ f > = - & a r ? % ) t ot _____ !______________________ Test Organism ^crHPAT\ M I LIKiQU!^outce4<u gT E) E ll/E R S cd ** Rac'd Batch _ 5 r g Z . Avg,Wt.Q ,Z S ( grams Avg. S ize a n Nb. Organisms per dose >^9 Loading Factor 7, 3 F ish Disease Treatment: 2/(.< W W /J A /fC j/ f / f r / S M / g /i T est S o lu tio n Volume Llte r a /A ll-G Ia s a Tank Tank Dimensions: 35x20 cm and 25 an deep Time S ta rte d / ) t> S ta rte d ___A/ / / A J & I - ___ Date Q p le ta d _ 9D ilu tion Water C arb on -filtered w ill wa (Temp. ^ _ - C D .O . g . pp, > In v e s tig a t (F u ll Signature) LC^ (95%aon*fid-engp_Lim its)-- `^ fa s ^ g a L 5& O M B IM C 8 ^ , . . CTT<~'(~S S h ~ 3> Y flP M ~ r A u a ll ^* * iS5: ^ C c l^ c . c n ^ p e> (7 ? -K * ^ /J L tarn NO. imULflA Ocx. ifM1VwUUn t T o tal No. t " 3 /1 Added Dead aR b ta l NO. pH D.O. T btal No. *** 1 Dead f* DbckSI D.O. TOtal No. P D .O . PP* Dead pp T r Control n tja i i k fi l 2 . & / /} P I T . CoM tX ____ -- o E2^ j 7 .0 > & Kgd.U/fde 2 ) fa j J . | Q m n \?ea r z> \ a2 B2 U ? .. & Z fi P3 IO 3 ad IO 7 1S K Z ft (hi 1L5 IfV Id fl 0 5 \ .fS A o _____ f t 7 / i A . i - s s n 2 f t r ______ S 3 2Ji d r o w 1 7 3/ 0 . 3 p f s i w w * K X i um m m & t o w s m e n s s9 J____I Li r c S S s r ^ m w f* T * rcrb m r Tn \T r.,a i n f rnr*r r jr . ^ 'u - s :u N ir . t fiF iif s ^ ^ ir e v tank No. syaptcew o r Reaction tm trol L 24-M r. OT -A l^ v 2R R R ^ a J U T r A C ik lA V i V t \ c >U f f "& K -K . ftM fer. 7M T. ' A kk - . ^ v K ^ L --------------- -- 96-ttr. -A i- k j BEST COPY AVAILABLE LNViKONMENTAL LABORATORY AQUATIC T O X IC IT Y DATA SHEET D E F IN IT IV E (FULL-SCALE TEST) LR PSfce Te3^ 3 ipWK.^ A o T t& x lftn Q f r t e r ia l T e s t e d ^ - .^ Test Qrgamsm^^ p j ^ j r - ( flE o S C fe * ___ L ^''//dJ.___^ tch *S -8J-- \vg. Wtf ) , 'iSs I grams Avg. S iz e ^ J^ t O j311 JiDu.Organisins per' doee ^ ^ Q Loading Factor f ) , _ ?is h D isease Treatm ent: ZXJU 4 < 22 f * t # e W A / / / E C / S f f l /fl/ / Test S o lu tio n Vblune ^ /^ L ite r a /A ll-G la s s Tank Tank Dimensions: 35x20 cm and 25 cm deep rime S ta rte d ) f i \ Date S ta rte d ______Date O orpleted____^ ^ / / g / d ilu tio n Water Carb on -f i l beared we: (t p . _ 2 (2_ * c. , d. q. , 6 . ^ Pt. ___ investigator IC j . (95% fir fS e n c e U n it ) g l L l U o / j __________ (F la il S ignature) C pW E.lM &'O 6 2 j \ * 0 ^ ?My> T a t f g r r <i AWS\ siuvi.qia NO. Gone. m g/1 SE Contro: i'J T re m u a L . J S O AVMS* Amount Added %--------- lD dG 24 Hours 48 Hours D o ta l No. 1 * D.O. T o ta l ND. P H I D.O. T b ta l No. PH D.O. T o ta l No. PH D.O. Dead P T Dead SL PP" Dead PP O * /y 1 lo t 1 ie l 1 1 O S2 h *> HL l M ^ y (mmAJMi & q S 3d ,Iq 1 0 2 3 QJ, 1 L flQ f l a c O b g H X /, 5 i n ,i y S i O . S C . J l S D iftli C Li tAp 5 L vU O 4 0^ b g %c n Hf'H. 61 M (Q /(O TT K \3 u 3d A 5 Z jO 3d d 2 dJ3 x o 2d ( 2 J I M . le a n , c 1 1 * /* nz 11_____ Z 3L_____ 1_____ Z d 3 f _____ z *L , VTSTM T H ^ r.W Y ilV ^'U V W tris ti n . g c . ^ u u i r ^ P ^ e 4t | fis * No. Sym ptom s o r A s a r i trin a x itro r bnw L z ic "l^ " -- 24- 1* . ^ B S G n r U B B S S T C H B c B r TM 1" ^ - TM 4t . 7M r . 96- 1* . e "*"* tv O O ,'.ic y A * w L^ =. ^ JL / irl^ A tL fv l f i - S lJ b A C iM ^ U P A c jN T l ^ r __ *k ,A s -------- L-- .-- ,.,.