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ACUTE TO XIC ITY TO FISH (FATHEAD MINNOW )
TEST SUBSTANCE_______________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-602. (1Octanesulfonic acid) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was 501. The test sample is FC6 0 2 -X . Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 61.05% water, 20% ethylene glycol, 2% Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 1% magnesium sulfate, 0.7% xanthan gum, 0.1% starch, 0.1% n-(3-chloroallyl)hexaminium chloride, and 0.05% benzotriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:________________________________________________________
M ethod: OECD Method 203 and U.S. EPA Method EG-9
Type: Acute static
GLP: No
Y ear com pleted: 1988
Species: Pimephales promelas
Supplier: Kurtz's Fish Hatchery, Elverson, PA.
A nalytical m onitoring: Dissolved oxygen, pH, and temperature.
Exposure period: 96-hours
S tatistical m ethods: Not applicable. LCso values not calculated based on
study results.
T est fish age: Juveniles
Length and weight:
Average length = 27 mm
Average weight = 0.15 g (wet)
Loading: 0.3 g/L
Pretreatm ent: None.
Test conditions:
Dilution w ater: Carbon filtered well water
Dilution w ater chem istry:
Hardness:
240 mg/L as CaC0 3
A lk a lin ity :
220 mg/L as CaCC>3
C onductivity: 500 pmhos/cm
Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Once
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Stability o f the test chem ical solutions: Not noted Exposure vessels: 4 L Pyrex glass beakers containing 3 L of test solution. Num ber o f replicates: 2 Num ber o f fish per replicate: 6 Num ber o f concentrations: five plus a blank control W ater chem istry during the study:
pH range (0-96 hours): 8.0 - 8.2 (control exposure) 7.3 - 8.2 (1000 mg/L exposure)
Tem perature range (0-96 hours): 20-21 C Dissolved oxygen range (0-96 hours):
7.6 - 9.2 mg/L (control exposure) 1.6 - 9.2 mg/L (1000 mg/L exposure)
R E S U L TS __________________________________ ______________________
Nom inal concentrations: Bk control, 100,180, 320, 560, and 1000 mg/L.
Elem ent values: 96-hour LC50 = >1000 mg/L
Element values based on nominal concentrations
Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS________________________________________ ___________
The FC-602 96-hour LC50 for fathead minnow was determined to be >1000 mg/L in both replicates, the highest concentration tested. Mortality observed w as~17% .
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number F 1 1 5 1 ,1988.
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OTHER____________ Last changed: 6/26/00
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BEST
COPY
AVAILABLE
FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST)
1 1 ' '- > 1
TYPE TESTl4r%
, .CHEMICAL TESTED FC - /aGZ X U ^ W a \ t r Ate P/uM/yt S d I
TEST ORGANISM
snuRCF.
PA 1 DATE REC'U / - fr g g BATCH N0._ L l& ?
AVG. WT-- AX-grams, AVG. SIZE____ 7 rm NO. OF OGRANISMS PER TRF.ATVF.NT L
LOADING FACTOR O ,.?> p/I.
FISH DISEASE TREATMENT /Vnne_______________
TEST VESSEL: W t w
fW K r
TEST SOLOTION V0LUME_2______ L
TIME STARTED dC CUT)__ DATE STARTED.3 r..gji.V___ DATE COMPI.FTKn
DILUTION WATER Carbon-filtered well water (Temp. &. 1 Cf D.O. 9. ^ mg/LrpH 8, A )
ANALYST^^SVA.^. d
LC^q(95% Confidence Limits! ^ JQOO
_____
Tank Initial Total _ __ 2_Hours__
48 Hours
72 Hours
96 Hours
No. Cone. Amount Total Nc. PH D.O. Total No. PH p.o. Total No. P D.O. Total No. p..
mg/L Admdoead* Dead
mg/L Dead
mg/L Dead
mg/L Dead
mg/L
X Control
o 8.0 V.L O S.o 7 b o fro ?.o O &D 7.S
n loo 3 o o
o R.\ 7,o O 2.0 h.b 7,<\ 6-6 O 7,1 1.?
Bo S iO o 8-\ &-Q D B.o 2 o 7.<\ 6.0 O 7.7 H.9
1-3 3Ao UcO. . o $ \3 O %.o J.g o 71 y.o O 7.5
)-H 5 h o 11,80 o fto >\ c . %.o 3..L 0 7b 2.3. O 73 Z b
.'-S' Jooc> 3oco o D
o B.\ 2 3 o 7-i I.i I 73 ! ' b
Test Temp. C
p^(~
Initials Date <AP> 2-3Z-H
3-\ ift 3-<M-89 i&B 3 -^ 5 `-5>s>
cawENrS:
,Test- Substohc^.
nn.<r;klg- oj t-flo Lutdgr.
Xm-hftl pH-
I&oq KfrqlLj = S.- R____________________
SECONDARY EFFECTS (OTHER THAN MORTALITY) ^
ianK No. Symptoms or Reactions
24-Hr.
TOtai NUUD<SrTCFSeST"
48-Hr.
72-Hr.
96-Hr.
l-l /-
/ /
/
y
/ /
/
/
1-3 Is xi-\V
)~1 IS
X0-V
.VnO-
1
f1 -
/
/ ""
/
Test methodology conforms with the following two test protocols:
(1) OECD 1203 "Fish, Acute Toxicity Test". (2) US-EPA IEG-9 "Fisl* Acute Toxicity Test".
/
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FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST)
a /`i w I
TYPE T E S T E R
CHB1ICAI TESTEDFT-koA* UMtr Aic
inr S o I
TEST ORGANIC
M \ * v^io SOURCE
DATE REC*nr I- ic -fi2 BATCH NO.jJ L S k
AVG. WT-- LJ.5Z.grams. AVG. SIZE__^ 7 cm NO. OF OGRANISWS PER TREATMENT Cr>
LOADING FACTOR O .3 <x/L
FISH DISEASE TREATMENT A/me._____________ _
TEST VESSEL: V l , U r P y t e * P b\q<><* f e e d e r
.TEST SOLUTION VCUHH-aS___ L
TIME STARTED./'/;3 <p ___ DATE STARTED .^-U-gg DATE COMPLETED
dilution wKter Carbon-filtered well water (Temp. # ) cf D.o._2i^_mgZL, pH X )
ANALYST.
( \ . /^vxA
Confidence Limitsi3 /OOP
I L,_____ _
Tank Initial Total ____ 2Jtours
48 Hours
72 Hours
96 Hours
No. Cone. Amount Total Nc. pH D.O. Total No. PH D.O. Total No. pH p.o. Total No. pH D..
mg/L Added Dead
mg/L Dead
rag/L Dead
mg/L Dead
ng/L
na*-
&
3T Control
O %.c H O > 7 'b O 3.C rf.p z> 8.0 p .o
2 -\ lO 3 o o
o
O 9. 7-3 7f\ 7 2 , o 77s .\
1 - 2 180 SVO
o B.\ 7 2.C
7A i - i o 7,7 H s
7-3 3 2 o <760 . o -0
O g.o H . o 7.1 2 . \ 0 ZH
SO IU&D
0 8.C L x O .. S-l Z 7
o 7. 3 . 0 O
73 i - i
io o o 3oo o o
to l.o O
S I 3 . Q o 7-1 j . \
i 73 1.7
Test Temp. C Initials 8 Date *6
A\ S i& >
U
3 -`-g
CX*MEffl,S :^ 3 r nhuiAuo-l
1<rs* .SuWs*rthC<, appAfenlly miSc/lole lui+lo looker
X d U!a \ pu- WtaW-E t\p.
J JW> nyt/*-J *&>-&____________________
SECONDARY EFFECTS (OTHER THAN MORTALITY) ^
lame No. Symptoms or Reactions
? -x
-3
24-Hr.
/ /
(y a
rotai NumDi3r Attectea
48-Hr.
72-Hr.
X/ / (L
\1
96-Hr. /
\V tL> ^ ..
7.... . ________ /
/
Test methodology conforms with the following two test protocols:
(1) OECD #203 "Fish, Acute Toxicity Test". (2) US-EPA #EG-9 "Fisl> Acute Toxicity Test" .
_n D \, a\/AII A R L 0021323 BEST COPY A V A ILA B LE
03/24/1988
ENVIRONMENTAL LABORATORY FINAL REPORT
1
LAB REQUEST NO. F1286
REQUESTOR NAME: RR ROBIDEAU/SAB DEPARTMENT: 0222 PROJECT NO: 06 DATE RECEIVED: 03/02/1988 DESC: CARBON-FILTERED WELL WATER - FEB. 1988
CONTRACT LAB: PACE
CONTRACT LAB COST: 144
3M E-L HOURS:
1
EXP COMP DATE: 03/23/1988
DATE COMPLETED: 03/23/1988
ORIGINAL SOURCE OF, WELL WATER, 3M WELL #2, ST. PAUL, MN.
SAMPLE DATE
CODE
DESCRIPTION RESULT
MIN DET LIMIT
03/02/88 ROOM 4
WELL WATER
COD SPECIFIC CONDUCTANCE AMMONIA NITROGEN - as N *PH
SOLUBLE FLUORIDE (PROBE) STANDARD PLATE COUNT TOTAL DISSOLVED SOLIDS TOTAL ALKALINITY (PH 4.5) TOTAL COLIFORM TOTAL HARDNESS
TOTAL RESIDUAL CHLORINE TOTAL SOLIDS TOTAL SUSPENDED SOLIDS
<5 MG/L 500 UMHOS/CM <C
17 COL/ML 280 MG/L 220 MG/L <1 C0L/10ML 240 MG/L <0.02 MG/L 280 MG/L <1 MG/L
* = CONTRACT LAB
tf^ o .:
SUBMITTED BY:
DATED : 3 ' , l / ' f ?
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