Document zQ0GE2yBgjjLXLo3EmND9qgjR

ACUTE TOXICITY TO AQUATIC INVERTEBRATES TEST SUB-STANCE (DAPHNIA MAGNA) Identity:Mixturecontainingperfluorooctanesulfonatwehich may also be referredtoas PFOS, FC-95, or as a component of FC-203FC. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-203FC, identifiebdy the laboratorays Rl 335. Currentinformation indicatesitisa mixtureof0.85% PFOS, 17% diethyleneglycolbutylether, 74.46% water,2.34% acrylifcoamer,1.7% alkylethersulfate2,.55% sodium octylsulfate0,.12% sodium nitrit0e.,09% sodium molybdate,0.85% triethanolaminea,nd 0.04% tolyltriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline202 Type: Acute static GLP: No Year completed: 1997 Species: Daphnia magna Supplier:In-houseculturesm,aintainedatASci-DETD, Duluth,MN. Analyticalmonitoring: Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethods: EC50 values calculatedusing the Trimmed SpearmanKarber method. The NOEC was calculatedusingSteel'sMany-One Rank testusing Toxstatstatisticsaolftware,VLz,,rsi3o.n1. Test conditions: Dilutionwater: DechlorinatedCityof Duluth,MN tap water,postcarbon filtereade,ratedfor24 hours priorto use. Dilutionwater chemistry: Hardness: Alkalinity: Conductivity: pH: 44 mg/L as CaC03 42 mg/L as CaC03 145 pmhos/em 8.05 Lighting:Cool-whitefluorescentlights.A dailyphotoperiodof 16 hours light and 8 hours dark was maintainedthroughoutthe testingperiod. Stockand testsolutiopnreparationA:1000mg/L primarsytocksolution was prepared by dissolving2.5g testsolutionin2.5L dilutiownater. Exposure concentrationswere prepared by serialdilutions. Exposure vessels: 250 mL beakers with200 mL exposure solution. Number of replicates:4 Number of daphnids per replicate:5 Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: pH range (0-48hours): 7.93 - 8.05 (controelxposure) 7.87- 7.92(1000 mg/L exposure) Temperature range (0-48hours): 20.4- 20.5 "C Conductivityrange (0-48hours): 145 - 161 pmhos/cm (controelxposure) 159 - 159 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48hours): 8.6- 8.9 mg/L (controelxposure) 8.7- 9.0 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Blankcontrol1,30,216, 360, 600, and 1000 mg/L. Element values: 48-hourEC,50= 653 (396-1077)mg/L 48-hour NOEC = 360 mg/L Element valuesbased on nominalconcentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIQNS The FC-203FC 48-hour EC50 was determined to be 653 mg/L witha 95% confidenceintervaolf396 to 1077 mg/L. The testsubstance 48-hour No Observed EffectConcentration(NOEC) was 360 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudy meets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportionin solution. REFERERQ.ES Thistestwas conducted by ASci Corporation,DuluthEnvironmentalTesting DivisionD,uluth,MN atthe requestofthe3M Company, Lab Request number R1335,1997. OTHER Last changed: 6/26/00. ASci Corporation/ASel-Duluth Environmental TestingDivision AScl Study ID# 5010-()68 STUDY TITLE ACUTE TOXICITY OF R1335 TO DAPHNL4 MAGNA DATASTANDARD OECD GUIDELINE 202 STUDY C-OMPLET D April 1, 1997 TESTING FA!QILITY ASCI Corporation/ASCI-Duluth EnvironmentalTestingDivision 4444 Airpark Boulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068 3M Company Lab Request# R 1335 Sponsor: 3M Company Sponsor Study ID# R1335 ASel Corporation/ASci-Duluth Environmental TestingDivision ASci Study ID# 5010-068 1-0 GENERAL UIFORMATION Study Title Data Standard Sponsor Sponsor'sRepresentative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilityDirector DefinitivTeest Dates Data Validationand Report Review Acute Toxicityof R1335 toDaphnia magna. OECD Guideline202. 3M EnvironmentalTechnology and Safety Services,Building2-3E-09, 935 Bush Avenue, St.Paul,MN 55133. Susan A. Beach, 3M Environmental Technology and SafetyServices,Building 2-3E-09, 935 Bush Avenue, St.Paul,MN 55133. Tel No. (612)778-7452. R1335. AScl Corporadon/AScl-Duluth Environmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811; Tel.No. (218)722-4040. Tina Galloway. Joe Amato. Donald Mount. March 25-27, 1997. Alan Mozol. R ports igna re: Retenfionof Raw Data and FinalReport Locafionof Raw Data and FinalReport Two yearsfrom the datestudy iscompleted, AScl Corporation/AScl-DuluthEnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID# R1335 2 ASci Corporation/AScl-Duluth EnvironmentTaelstinDgivision ASelStudyID#5010-068 2.0OBJECTIVE(S) To deter7nitnhe48-hECSOand48-hNOEC (noobserveedffecctoncentratiooftnh)etest substancRe1,335,forDaphniamagna. This studywas conductedaccordingto theASci studyplan retainedby theSponsorand OECCDD Guideline202 (OECD 1993). 3.0TEST METHODS TestSubstance Test Organisms DilutionWater Exposure Chambers Propenies:R1335, amber liquids,pecifigcravity1.0(water= 1),stable, and misciblewithwater. Testconcentrations0: (dilutiownatercontrol),130,216, 360, 600, and 1000 mg/L. High testconcentratiosnolutionpreparedby dilutin2g.5 g of testsubstancein 2.5 L of dilutionwater. Lesserconcentrationwsere preparedby directadditionof highconcentratioinntodilutiownater. A 0.6 dilutionserieswas utilized. Source: Healthy,lessthan24-h oldDaphnia magna neonatesobtained from ASCI stockculturemsaintainedinwaterof testdilutiownaterquality. Use intest:Gravidadultsisolateidndilutiownater24 h beforethetest initiationU.sed 5 organisms/replicaatned 4 replicates/tceosntcentration. Handled witha smooth borepipetwithan insidediameterof 6 mm. Did not feedduringthetest. Source: DechlorinateCdityof Duluth,MN, tapwater. The most recent chemicalanalysisisin Raw Data Package. CharacteristicsA:t testtime,thewater had a hardnessof 44 mg/L (as CaCO3) and a pH of 8.05. Aeratedfor24 h priortousinginthetest. 7ype: 250-ml borosilicatgelassbea -rs. Use in test:200 ml of dilutionwater or testsolution/chamber.During the test,keptcovered,exceptwhen experimentalobservationwsere made. Sponsor: 3M Company Sponsor Study M# R1335 3 ASci Corporation/ASel-Duluth Environmental TestingDivision AScl Study ]D# 5010-068 Incubation Observations Endpoint Calculations Duration: 48 h. Dailyphoroperiod: 16 h lightand 8 h dark, maintainedusingcool-white fluorescenbtulbs. Temperature: 21 OC. Biological:Daily- gentlyagitatedtheexposurechambers and then counted immobilizedand dead testorganisms(immobilizedorganismswere those thatdid not swim within15 secondsafterexposurechambers were gently agitated)D.id not remove any daphnidsfrom theexposurechambers at 24 h. Water chemistry:(1)At testinitiatiotno-talhardnessand alkalinity(,2) daily-temperaturep,H, DO, and conductivityA.lldeterminationwsere made accordingtoAPHA methods (1995). EC50: Trimmed Spearman-Karbermethod. NOEC- Steel'sMany-One Rank Test (TOXSTAT statisticsaolftware, Version3.1,Universitoyf Wyoming). Sponsor: 3M Compitny Sponsor Study M# R1335 4 ASel Corpomtion/ASci-Duluth Environmental TestingDivision ASc.1Study [D# 5010-068 3.0 RESULTS Test SubstanceToxicity(Pertinendtataare in Table 1) 24-h ECSO: > 1000 mg/L (notcalculable). 48-h EC50: 653 mg/L (396-1077). Ranges of Water Chemistry Parameters 48-h NOEC: 360 mg/L. Totalhardness (as CaCO3 mgll): 44-46. AlkalinitY(as CaCO3 mgll): 42-46. Temperature (OC): 20.4-20.50C. DO (mglL): 8.6-9.0. PH: 7.76-8.05. @Le conductiyLQ@mhe, c@m 142-161. 4.0 TEST QA/QC QA/QC Criteria Da Test organism dead, immobilized or trappedin 0% dead, immobilizedor trapped. controlexposure must not be more than 10%. The lowest DO concentrationattest terminationmust be at least60% of theair saaturationvalue attesttemperature. The lowest DO concentrationmeasured was 97% of theairsaturatiovnalue at21.0-C. Test durationmust be 48 h. RFRoepeefafsi,eirencesubstancetoxicittyest (thistestis optional). The testdurationwas 48 h. CurrentcontrolchartforD. magna acute testingisincludedin the raw data package. Sponsor: 3M Compitny Sponsor Study [D# R 1335 ASci Corpomfion/ASel-Duluth EnvironmentaTlestingDivision ASci StudyID# 5010-069 5.0REFTRENCES American PublicHealthAssociation(APHA). 1995. StandardMethods fortheExaminationof Water and Wastewater. APHA, Washington, D.C. Hamilton, M.A., R.C. Russo, and R.V. Thurston.1977.Trimmed Spearman-Karber Method for EstimatingMedian LethalConcentrationsinToxicityBioassays.Environ.Sci.Technol. 11(7):714-719C;orrection12(4):417(1978). OrganizationforEconomic Cooperationand Development (OECD). 1993. OECD Guidelinesfor Testingof Chemicals.OECD PublicafionInformafionCenter,Washington, D.C. Universityof Wyoming. TOXSTAT Package Version 3.1. Sponsor: 3M Company Sponsor Study [D# R1335 6 ASci Corporation/ASci-Duluth Environmental TestingDivision ASci Study ID# 5010-068 TABLE 1. ACUTE TOXICITY OF R1335 TO DAPHNIA MAGNA Test,submwce Concentration 0 130 216 360 600 1000 CumulativNeumberandPercenotfDeadandImmobilizOerdganismse 24 h 48 b 0(0) 0(0) 0(0) 0(0) 2(10) 4(20) 0(0) 0(0) 0(0) 1 (5) 10(50) 13 (65) Percentages am in parenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFTDENCE INTERVALS ECso and NOEC 24-h ECSO 48-h EC50 48-h NOEC Data (mg[L) > 1000 653 360 95 % Confidence Interval Not Calculable 396-1077 Not Sponsor: 3M Company Sponsor Study ID# R1335 7