Document z42Dk71ygyNQdoj3OVaLv4Ga

3 .4 W E V 1? 79 iiD (Smj&MCD1807 E. d u P o n t d e N e m o u r s & C o m p a n y IN C O RPO RA TID W i l m i n g t o n , D e l a w a r e 19898 POLYMER PROOUCTS DEPARTMENT EXPERIMENTAL STATION PERSONAL AND CONFIDENTIAL cc: A. J. Dahl B. W. Karrh L. J. Papa Pral File I.C. - 353 - N11400 - 269 ^4AZ7i,'/57$ ' May 20, 1981 TO: DR. C. WALKER - PPD, Chestnut Run FROM: S. S. STAFFORD ' ANALYSIS OF BLOOD SAMPLES FOR FERFLUOROOCTANOATE (Job No. 811-610; PRAL Nos. 81-1763-1777; Notebook Nos. E22514,E26238) As requested in your letter of 4/23/81 to L. J. Papa, the 15 blood samples submitted then have been analyzed for perfluorooctanoate (Cg). Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with e detection limit of 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Key Words: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC There s a w o rld a1 thin gs we re doing som ething about 000081 EXP000018 EID713832 TABLE I CONCENTRATION OF PERFLUOROOCTANOATE IN BLOOD (a) Sample PRAL No. 81-1763 81-1764 81-1765 81-1766 81-1767 81-1768 81-1769 81-1770 81-1771 81-1772 81-1773 81-1774 81-1775 Bl-1776 81-1777 Date Sampled S.S.No. Name 4/24/81 195-34-9680 4/24/81 221-26-4482 4/24/81 221-22-3017 4/24/81 137-24-3476 4/24/81 4/24/81 222-24-4159 202-18-9533 4/24/81 222-30-6425 4/24/81 222-34-1611 4/24/81 222-38-1332 4/24/81 190-22-3702 4/24/81 242-14-1512 4/24/81 218-32-8049 4/24/81 214-22-6295 4/24/81 236-74-5332 4/24/81 491-58-2410 GC Analysis___________________ (b Date Analyzed [Cr ], fig F/g b loo 5/6/81 5/6/81 5/6/81 5/6/81 5/6/81 5/7/81 5/7/81 5/7/81 5/7/81 5/7/81 5/8/81 5/8/81 5/8/81 5/10/B1 5/10/81 n.d. n.d. n.d. n.d. n.d. <-007 n.d. n.d. n.d. 0.009 <.007 <.007 0.043 <.007 <.007 (a) Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81,), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 pgF/g. The detection limit is n, 0.004 ygF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cg] ;< 0.004 ppm, which cannot be distinguished from reagent background. *REDACTED* 000082 EXP000019 EID713833 2-45 RCV12-7 [STABL&HCP'SOi E . J. d u P o n t d e N e m o u r s S C o m p a n y IN CO RPO RATFO W ILM IN G T O N , DELAW ARE 19898 POLYMER PRODUCTS DEPARTMENT EXPERIM ENTAL STATION PERSONAL AND CONFIDENTIAL cc: A. J. Dahl B. W. Karrh L. J. Papa Pral File I.C. - 353 - NI 1400 - 269 >M?26-/S7? Hay 20, 1981 TO: DR. J. M. HEDGES - PPD, Circleville, Ohio FROM: S. S. STAFFORD ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 810-633; PRAL Nos. 81-1936-1950; Notebook Nos. E22514,E26238) As requested in your letter of 5/1/81 to L. J. Papa, the 16 blood samples submitted then have been analyzed' for perfluorooctanoate (Cg). Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of 'v 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment 3ah Key Words: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC T h t-rc -'i a w o r ld o ! Ih i'-o s wc- rp d o m o s o m e tb in o a b o u t 000083 EXP000022 EID713836 TABLE I CONCENTRATION OF PERFLUOROOCTANOATE IN BLOOD (a) Sample PRAL No. Date Sampled 81-1936 5/1/81 81-1937 5/1/81 81-1938 5/1/81 81-1939 5/1/81 81-1940 5/1/81 81-1941 5/1/81 81-1942 5/5/81 81-1943 5/4/81 81-1944 5/4/81 81-1945 5/4/81 81-1946 5/4/81 81-1947 5/4/81 81-1948 5/4/81 81-1949 5/5/81 81-1950 5/5/81 81-1951 5/5/81 P.R.No. 1485 3518 2430 3032 5656 6828 3322 0275 2718 3766 4748 5646 7350 1505. 5645 7452 Name GC Analysis Date Analyzed 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/13/81 5/13/81 5/13/81 5/13/81 5/13/81 5/13/81 [Cft], PE F/g bl 0.027 n.d. 0.017 0.015 n.d. n.d. 0.030 0.027 0.025 n.d. 0.017 0.026 0.028 0.008 0.053 0.017 (a) Analysis as described in Lab Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 ygF/g. The detection limit is ^ 0.004 pgF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cg] < 0.004 ppm, which cannot be distinguished from reagent background. REDACTED* EXP000023 EID713837 000084