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AZI-LIA EXPANDED RESEARCH PROGRAM
Amu k j m Zin c In *t t t v t t * In c
k iu u m r. lumt P*.rtw W liMi txftj fwmui
rn MADISON AVENUE
NEW YORK IT. N.Y.
Lj u o Ixovrnua Auoatnon, In c
m 19C3
IN KNY WftJI TO wwir0-
Tot All Nutxr* of tb* B**lth Md Sfty CaolttH
Frcat Don 0. fwlir, XUBO
mcti Toatlolty t**tln* for artpo-l**d flapood*.
toelorurwi. For jour rrrln, toanli *od vot* or* tb* fallowlaci
(1) hggoul >0. 1 lb* Bio* UbontorlM toe.( Boo FnnelMO, California. Dated Ju m 17# I963.
(S) PrepoMl lk>. t - Xodustrlal *lo-T*t Laboratorle*, Za*.( Borthbrook# ZUloolo. Dated Jua* 12# 1$].
(3) Fropoaal Bo. 3 * Induatrlal ByyUo* Foalttla of Aaertoa, Xas# mtebr*h, luuTlYiBli# tatot Deoa*ber 17, 1$$.
(b) Fropoool Bo. b Woodard Reeearch Corporation, Berodoo, Vlrflala. Dated April 2$, 1963
At tbo Bealtb tod Safety CoBlttN a**tln* 00 Flbnur 89, 1963# )W
*111 r*o*ll ** dl*cua**4 tb* *er**nln* of our o*v or*mno-l**d cnfoundj
for toxicity. At tb*t tic* * bod proposal* tram tvo labor*tori**
ecnrloj tbl* ort and It v*J flt by tb* a*ad>*r*blp tbat additional
labor*tori** abculd b* cootartad end Invited to Mad la prcpo**!* for tb*
cooduot of tb*M taata. Aoccrdla*ly, X aollcltad tbr** crtbar labor*toxic*
end obtained tvo additional propoaal*. Baob of tb* foor labor*tori**
Yjbalttlnr propoMl* baa baas vialtad and lnv**ti**t*d by a* a* to
competency, capacity, eo*t# *ad ataodln* la tb* flalA.
Vrle* Nr
fri--rr of tb* Fro|io**l
Compound
1. tb* Ulna Leboratoclee Ice., tea franc! *00,
Ctllforftlti
Scrtenln* taata lenludin* Inputto* and
kla abeorytlom.
|330.
(lot reduction for 6 or Bar* *c*pound* remitted *t
tb* MM tla*.)
I. Xaduatrial 8lo-T#t labor*torlaa, Borthbrook# Illlnolai
lteof**fIndia* toxlolty t*t* for la**tlon
and *kla abaorptlcn.
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3. industrial Byciene Foundation of Anerlao, Flttehurgh, Feonoylnalaj baa*5-findlag totte of Ingeetlon and Ohio ohoorpttcc.
Price hr Coopoun^
|k00.
k. Woodard Baoeorch Corporation, Bonded, Tlrglalej Hugo-flading totto of lageotloo dad eUa ehoorytlcn.
tlSO,
Ercrnindaticni Vo neeraiend roar ftrortblo oceeIdoration of propoeel
o.'I frcoi lie lino lebonttrlee end propoeol >0. t tram tho Induetrlal
Blo-Tmt Uboretorleo, Isa., tar tito follevlag neaanoi
1. Both lehcretorie* on dlnoted by aoapetent lndlrlduelj tod etaffad with experltooed ood ea^eteot help.
t. ttelr prlooo on to lino vlth vbat jroa would expert to pay for thin oorrloo.
3. Cto Dlnctan of Both lohontorloo on engaged la dolly actlvltlee ldileh Include o vide range of lood analyoeo and onliatlcoo oa the Induotrtol ocooo. ftle U dot trot of tho other tin lohontorloo. For lnotoaee, tho Voodard laboratory boo no load Work going on at tho pnoont tlao end then quertlomd von obviously anfoaillor vlth tho dotollo of load enalyeee, and vo bon no doeIn
to 001lot la the education at our estate of 0 cantrclol lobontory.
k. fnllnlnary oononlnc of thooo notorlelo la only tho
flnt otop la ooy evaluation of oa^oundo vhloh acy bon mnointol
applloatloo oad It vlll ho nil to 00loot lohontorloo vho ban tho
capoblllty to oerry oo eubeeguent tooting ouch 00 npor
end
cyo lrrltotloa vtoo then mluotloeo on aoodod. Both of tho lohontorloo
vo noctud bon ouoh eopahllltloo.
3* Vhen oar orgaoo>lead coogousda bon donlopod o ocontrclal fooolhlllty. It vlll ho ooootoory to foaanto eortala toxlalty data to
ho idltted to nrlout (cmnant agoacleo to tetiefy tho nqulronoato of ouab agaocleo. Both of tho lohontorloo vo no mend bon the kaoo-bov to gmonte tho aaooooory deteeod to soldo uo la obtaining tho Bocetoory gonraaent opproral.
Ftodo oad Booanoodotlcnoi Vo nnrottoiid your approval of Propooala >0. 1 oad lo. 2 00 that either 'or both Mjr ho uoed la pnoont end avboo^uont crooning and toxicity tooting. funding vlll ho oo aoooooory to eonr tho ort>er of ocapouade to bo tooted.
Den 0. Pooler froloot Mtaagor
M*> Don 0. rwltr
International U*d-Hae B*rfa Orpuslwtina S92 M41*ca Arum nr Tort 17, hr Tort
> X awm** (So BO* apjnon) tb m of 1b* Um
UborstorlM and tb* XoSaatrlal llo-Urt Laboratorl*# a* Tro>ct
U! 79-63, "Toxlolty Twtlsc for 0rfoo-t*S Ccapotada,*
Sa*t________________
Cannon
iu a s i mns* ms s au o t *t rax aj, 1563.
---------------
Dates________________
N 191.01
reoroau. ao. 1
tst nn UBonATOftm wc. IWeeerch aod Pevelbpaeat
109} Folam Street - Baa Francisco 3, CalIToral*
Kr. Ooo 0. Fowlor Usd Industries Assoolatloo 09$ thdlaoo Avenue Ike Tort I?
Jw 17. 1963
DMT fiaM
It *u a greet pleasure to visit with you lut w*ek end to maw oar friendship. Our prrrloua york la the Air Follotlao flolA w. soot enjoyable. I treat that wa say find a flail of Joint affort la the setters of laaA end the Lead Industries. I hare always adatrwd the wort of these Industrial aaaoclatloaa such aa AFI aad laad Industries, asd bare fait that they ara flat examples of tba Aaarlcao tradition of both coapetltlcn aad cooperation,
la regard to tba oattar of developing laforaatloo for tba aafa
at
saw load products, air laboratory would ba aoat happy to work la thla araa
with your group aad wculd ba pleased to aaka a proposal for your coosIdaration
r1stIra to tba axtaat of wort sfelcb Obould ba daoa aad tba coats Involved.
Aa 1 understand your Loaadlata problem, tbara ara a tudnr at art laad
cotcudl la varying degrees of development shoes toxicItIts ara la general
unknown. Because of your long axparlaaea with organic laad* and knowledge
aa to tba much higher toxicity of tbaao compounds aa ocx^arad with diffaraot
laorgaala leads, It doss seem appropriate for you to bara determined tba
propsr ordsr of aagnltnds of toxicity ao that suitable handling precmotloM
aa bara barn daralopcd for both types of compounds could ba applied. X
would suggest, therefore, that consideration bo fIren to daterainlog toxicity
by oral and percutaneous routes for all compounds, and that of toxicity follow
ing Inhalation by ccapcsada which nay bare a significant rapor pressure. At
this stage only order of aagnltuda figures need ba obtained, and wt propose
to use our nodlflcatlon of the standard nethod of Celchasm, la wblch tba
and LDiq o
be bracketed by adalnlstrst loo of the cosgouods orer a
range of doeea or concentrations. At such ties ao further Information la
needed concerning the ccapomd, nort precise values ahould be obtained la
order to better define the toxicity aad otbs r testa would bara to bo performed
to amfoxB with the requirements of the Baurdou* distance labeling Act.
Ve ara appending our recemendations for eeco^lleblng this preliminary toxicity evaluation and the costs relative to these. Ue ahould Ilka to point cut that our Laboratory baa bad considerable experience la tba handling
of dangerous and highly toxic compounds, Including explosive saterials, aad that cur staff, therefore, la eaapeteat to handle eoapouade of any degree of toxicity aad potential heaard.
O
N 191.02
se. i.:
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Tbd Ilaa laboratorlaa
Mr. Dae Q> fowler h<i Two 6/17/63
It la alweya aora eocaaalant and laae arpanalve to work with aarlaa of ccayopda, and for that raaaoa our laboratory glrae a lOf dlaoouat on elx or Dora aanplaa ei4itted at a aln^la tlx*. Va ara la a poeltioo to atart work on any toxicity taat vlthln approxlaetaly too daye aftar receipt of the eaepcuDd or aotlfloetlce of lta aOl^atat.
Va would be aoat placard alao to be cooaldared la eoooactloa Vita other etudlae la hlch ycwr laboratory al^t aollclt tha aid of a profeaelcoal group iuch aa cure, larludlnc the awaluatloo of tba toxicity of pelata*
X aa looking fonaxd to further pleaeant aaaoolatlooa.
Tory truly youre,
Slgnedr Charley
C I* Bin*, M bij AH Clrwetor Tba Blae labormtorlee, lae.
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znorosu. k * m u *d ncumuja nunvi t o renDcaujar renteITT BCKSEtPt} FR&JZCT8
I IntraraetrlB toxic ltn Group# of tvo rat# (either eex,
lc6 gat. or aore la wight) #r# #lrru eultable do##
leeele eltb to lacreacat of 1.5 la order to bracket
U>n end tt>ii-o. Cuaulatlra aortalltle# #r# determined
#t Zb, b8 #od l8 boura. Total eo#t
41C0.00
II. Zemrteneoue toxicity; Oroup# of two rabbit# (either
#e*7"5FTg*r'or"liorla wight) #r# gleea aulttbl#
do## lare la with #a increment of 1*5 la ord*r to
bracket tb#
#ad IDino- Cueulatlre aortalltle#
ar# determined #t Zb, totad 16& boor#. Total coat
1)0.00
XU.
Vapor Toxicity; Orotrpe of two rat# (altbar eex, 100 or more gma. la velit) ar# expoeed for oo# boar to aultabl# concentration# with aalocraaaat of 1.) la order to bracket tbe LOq end LDv j q . Cuaulatlea aortalltlea ar# detenalaed at Zb, So ead 16S hour#. Total coat
ZOO.00
Coat for all three terte, per oompound
Coet for tbe oral ecreea #od penrrtaneoue, par ooaiouad
10)1 reduction for alx or aor# ocapound# eifealttad at tb# Haa tla#
t)0.00 2)0.00
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mWTKUt BI0-TS3T LABOW.TORII8, UK. i6io rrcote* Doca PorUsbrook, Illloala
telejfcooe CSeetwood 2-J030
dune 12, 1963
Hr. Deo 0. roeler Prelect Manager lead laductrlaa liKcUtlta 292 Mltot Avaoue * York 17, In York
taw Dow
V* *U1 ccoduct acute oral aad acuta pereutaaacua tcxlclt/ etudlae
aa par our propoaal of February 11, 1963.
Yha ody chaoga vlll be la the acuta parcutaoaoua teat la tdilcb two rebblta vlU be uaed at each of 3 doee laralj.
Ybe 07a lrrltatloa and acuta Inhalation taata ara not of prlaa lo-
portaaoa Initially. Su m taata aay La dooe Later it further Interact la a particular eoapouad dcreLope.
The coat for theaa taata vlll be $275-00 par aatarlal If a alalaa of flea aatarlals ara attained at the aaaa tiaa.
Sincerely four*,
SljDedr lea
JCCipar
1. C. Calaodra Director
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CKUJTRIW. M0-TE3T UB0ftAT0AXB3(IK. 1810 frcct*** Rc*&
ortbbrock, tlUaola Tolojbooo CRootvocd 2-30JO
fobrusry 11, 1563
Hr. So d 0. Totltr, Project Moosgsr Looi Io6u*trloo JUooclottos 29? Ms41oco Arraus kv Tort 17, > Tort Boor Mr. Torlorj
V* oj-o ooelools* our propoool for nac*-fla41af toxicity toots 00 sotortolo Is ublcb your tsooclstloo 1* lutorootoA. Tbs coot of Uk m Woto vlii bo *600.00 por sotortol tootoA. If you bon soy quootlaes 00 tbooo procoduroo plosoo 4o not boiltoto to cull uo. Tbort you 040I0 far pmltttac us to aiAaslt our prcpooolo.
Slseoroly,
StgooO: 3. c. Coloodrm
J. C. Calsadim Mroctor
t
St; e. .v *
Auof-nsDW foxxcm nwm
X. Wanaa-Mpdlna Acuta Oral Toxicity - Albino Bata
A Acuta Orul Toxicity - Albino Rata kaaltby, young albino ratr of tba Bpragua-Darlay attain vitk
a a-raruga body uuigfct of approximtaly IDO g-un ara u*ad aa taat tba rata ara dlrldad Into group* at tour aolMl# Mob far doalag purpoaaa.
All m1m)i utl ara kapt under obaervatloo for fl*a daya prior s to axparlaantal uea, during ublcb ported ti*7 or* ebackad for groaral
Jfeyalcal vtll-balng asd baaoprorlty. tba anlaalr an houaad indirldually
la (took cagrr ml pernlttad a etanderd laboratory tat dlat* plaa otw
ad llbltoa uatll 16 boura IwnaiHataly prior to oral intubation.
' i
Cu tin aornlng of tb flrrt tort day, afur a 16-hour fart (uatar patuittad), tba at lactad doaa groupa of four rata aaeh art Istvbatad ultb
prarloualy caleulatad doaaa at tba tart aatarial. All doaaa at* adalalatarad
dlraetly Into tba atraarhi of tba tart rata ualag a bypodanla ayrlaga
aytppad alth a ball-polctad Intubating Barilla.
'-j3 v;.-3
Pollorlng oral adalnirtraticocf tba tart aatarial, tba rata
J ara ratumad to tbalr atock cagaa and ebaarrad for tba auocaadlag lit daya. All algnlflcaot raaetlooa aad aortalltlaa Hch occur ara raeordad.
Particular attastloa la glvan to tba obrarratloo during tba tint toot
houra aftar imitation.
At tba aad at tba' obaarratlon parlod, all data ara collactad rod tba ranga-flndlng acuta oral atu latbal doaa (RIUm) of tba taat
aatarial la caLculatad ualag tba taahnl ipiaa of Vail**, tecajauu***, od lbc^soa aad Vail****.
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Roeklaad M Mat, nooklaad Barra, lav City, lk Tartu
* Vail, Carrol S.t Tablaa for Comaalaot Caloulatloo of Nadlaa* . ZffactlT* Doaa (IDjo or BVn) aad Xnatruotlooa la tbalr tba* ; Pleartrlea. Bapt. ly}*.
** Thoapacn, vtlllaa R.i Oaa at Horlng Avaragaa aad Xntarpolatlca to latlaata Madlaa-tfractlva Doaa. Pact. Bar.. Bor. 1967.
.a ! **** Oxwpacu, VllUaa t. and Vail, Carrol 1 Oo tba Ccoatrvatloa
of tablaa for Moving Artraga Xatarpolatlao, llcaatrlca. March
titL&hU&i.xiMaL
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n. bacra-rindlca Acute Fercataneoua Toxicity - Albino Eabblta
Toung adult, *ev Zealaod (train ad* albino rabblta ara aaployag aa taat aainala. Tba body wlgbta of tba rabblta uced range froa tvo to three kilograna. All rabblta ara maintained under Obaervatloo la tba laboratory for five deya prior to teetlng. Carina tba pre-teat parlod tba anlaala ara examined vlth raapact to tbalr general baaltb and eultablllty aa taat anlaala. da rabblta ara bouaad la (lock cage# and aalntalnad oo tba ataadard laboratory rabbit ratloo*, food and aatar ara panalttad ad imtta.
Tvanty-four boura prior to tba daraal applleetlcne, tba backs of tba rabbit# art (baaed fraa at hair vlth alactrla ellppara. tba anlaala ara than returned to tbalr atock regee to aaalt teetlng an tba follovlag day. tba 2b-hour uniting parted allowed raeorary at tba (tratua eorbtia fro tba dlaturbanee ableb acccupenlee tba clipping prooadura and also parmlta beallng of any alcroecoplc abzaalooa poaalbly produead daring tba proeaaa.
Co tba taatlni day, tba daraal doaa for aecb taat rabbit la aalgbad aoomtaly an an analytical balanea. Applicationa at tba tact aatartak ara aeda In eolld font oo tba axpoaura altaa of tba rabbita. tbraa doaa levale aaplngrlng four rabblta aacb ara oaad. After aaeb applloatloo, tba axpoaura alta la covered by napping tba trunk of tba anlanl vltb aa lapervloue plaatlc (beating uhleh la aacuraly taped In place, tbla pleatlc wap laeuiaa IstUate ocetact of apldatala and taat net#rial.
Tba tart Material taoalaa In ecetect vltb the akin for 8k bom. behavioral reaction# ara Obearued and recorded during tba contact period, aftar ableb the plaatlc abeetlng la moved froa each taat rabbit. Tba axpoaura altaa are axaalaed for local reaction# and the enlle raturoad to tbalr atock cage#. At tbla tlaa It vlll alao be poaalbla to obtain aa oatlaate of tba prlaai7 akin Irritating propartla* of tba taat aatertals. (fceervatleoe for mortality, local raactlooa, and behavioral abnarnaUtlee ara continued for a total i* lb daya follovlag tba akin appllcatlcoa.
At tba and of tba obaemtlea period, all data vara collected aai tba range-finding acuta parautaoaoua naan letbal doaa (WUjo) of tba taat natarlal la calculated tttlng tba tacbnlguaa of Vail**, Pxn^am^**, and Tbcageoi and Well****.
focklaad Pabblt Mat, boablnod Tana, Saw City, fa* leak * Sea footnote**, page 1 * gaa footnote***, pag* 1 ** Sea footoote****, page 1
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lauta, fthaUMcq Trlclty . Allilno.Rata
Young, Adult albino r*U at tba Spragua-Iavlay atraln ana aaplayvd aa th* Uit aalnala. Pre- and poit-Uit cm nod otaenratloo of theea rat* ar* aaaantlally tba h m u that already daaorlbad for rati uaed la tba ranga-flndlng acuta oral toxinlty taat (aaa p. 1). Hovaver, anlaala uaad for Inhalation expoaura ver* sot faatad prior to taetlng.
flva proupa at tour rata aaob ara utUltad la tba rtudy at Vat tort atarlal. lit inn<i body weight at tba taat anlaala na approxlaataly ItJO gran*. tba inhalation exyonurwa arv conducted la a miltlpl* labalatioa
chanter deelpoed for alaultacaoua axpoaur* to ataoajbarc aaturated and fractionally aaturated vltb tba tilt cat*rial, a. g. 1/2 MtoaM, lA
aaturated, 1/8 eatuintad aad 1/lfi aaturated at roan tcacuratuie (85^5). lb# tMt 1* dealpned to na tar a four-hour period.
At tba end of tba expoaura period, tba rata are dbaarrad for tba ` ueeeedlag lb daya.
IT. Haage-flndlm tve Irritation Teat - Albino Ibbblte
A croup of flat alb loo rabbit* era aaad to evnlaate tba apt Irritating propartla* of tba taat aatarlal. tang adult, *w Zealand attain <"'
an ealected for tba taat.
The taat Method alleged la pattarnad after that of teniae at al.
exactly 50 ag of aolld taat aatarlal la loatUlad loto tba <xi>mctlval
aae of tba right aja of each teat rabbit la a croup of five anlaala, tba
left apt of aaob
aarraa aa a aoorlag control.
Oaa, #A, AO, ft, 9$ boura, aod 7 dapa following tba Initial lnatlllatlacj, tba cornea, lrla, aad palpebral conjuncture are enlaed Individually aad radad for Irritation aad Injury according to a etaodard eecrlnc ayetn*. tba narlnn poaalbla acora at any ooa examination and aoorlac period la 110 polnta tdilcb ladlcataa narlaal irritation aod lima pi to all thiea ocular tlseuae. faro acora ladloetee no Irritation fatanr.
After tba coagulation of tba teat, tba acoraa are analyiad aod n detcrlptlva aye Irritation ratlog la aaalgoad to tba taat aatarlal. tba criteria need fer aaalgnaant of tba deecrlptlve rating are tba frequency, tba extant, aod tba peraleteace of Irritation or daatge rblcb occurred to tba three ocular tlaauaa. la tba olaeelfloatloo ayataa naad, apaetal anpbaala la placed upon Irritation or liana pa to tba cornea. Correepoodlngly lota etreee la placed upon conjunctival aod lrldlal affOota.
Tba daacrlptlva ratine naalpnad are ooa of tba following, aaob of rblcb abareoterltee n particular level of otbtbalado Irritation aad Senegal
Soo-Irrltatlng fmetleally *oc-Irrltetlng Mlnlaally Irritating Kildly Irritating Moderataly IrrltatUf daveraly Irritating Cxtravaly Irritating
A 'Method* for the Atu^oJ^lrrttatloo'aoJ Toxicity of tubetaooca Applied
Topically to tba Skin aod Masoua Maabranae*, Crate*, John Woodard Oaoffray, aad Calvary, Herbert 0., J. Ibarn. A Zxp. tbar. w,A, December 19k<.
u^iua^pncwigw. .pgMMfx., ,.,iuu 1
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Hr. Louis cappslll, Protect Mtoacar Expaated Beasarcb Procrsn Lead Industries Association Aaxrloan lino Institute, Zoo. 892 Mill Hon XnoH
sr Torfc 17, sw lock .
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Dear Mr. OqfiUli
In aeoordaaoa with our dlscusstca than you visited u j here In Pittsburgh, tbe fallowing la u outlls* of tba range-finding testa, lnforaatioo va should Ilka to hava on tba ccapouad, aoS tba tlaa raguirad for susb studies. Tba chargss are listed for a slngb tart oe single ao^oaM and tbs total cbarga than all taata ara Bate on a slagla ciapinJ.
The charge for tba cox?late range-finding study, thick la* olates (Individual tart cbargta la parantbasas)t
1. Tba daternlnatIce at tba slr*la doaa oral adalnlatratloa IDJO for aala, albino rata (|17))(
8. Tba datanlaatlea of tba akin paastrstloa ZCJO for aala, albino rabbits baaed upon a alalia oon* tlnuoua Abour eontaot with tba Intact and abtadad akin ($22}),
3* A single oootlnuoua labalatlco exposure of aala, albino rata to a a Ingle aoooaatratloo for a period not ivnasfllrg eight hours ($173),
b. Tba tetanalaatlon of tba prlnary akin Irritation aoora thick la an evaluation of tba affact of tba continuous exposure of tba intact aad abraded akin of aala, albino rabblta for a aaxlnua parlod of 8k boon ($73), and
3 Tba avaluatlon of tba syt Irritation produosd In aala, albino rabbits ($T3), la $700.
Zt would expedite our tartins if you could supply as auob of tha following data aa porrlbUi tba structural foraula, tba aolaonlar valtfit, tba apeolflo gravity, tba boiling point, tba vapor ptaaaura, tba stability at roc* tngarntura. aad tba solubility aspeolally In vatar, organic solrants, and corn oil.
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N 191.04
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XSDCBTMAt ROZBOC r*JE*3X* Of AMKXCA, XK.
Hr. tool* Cappaili Cceotor X7 196a hui
Dm tMl that ptriod raqulrad fro* tha tlaa of tha raoalpt of tha ohaalcal to tha data of tha aifbalttlnf of tha final raport la ataao to alit vaaka, ahtch lncludaa a ooa-to-tvo vaak parted for tha laboratory acollaatlxatloo of tha anlaala and a tvo-vaak poat-a*poaura parlod for tha avtluatlon of tha poaalbla dalayad affacta or tha raoovar7.
Slaearaljr jmn,
ftlDa4t ns
>. I. Sohrank, JhJS. Mtna^fnt Dlxactor
BSida
cci Mr. Don 0. fwltf
'K"xr fci
Trr-Tr*'''
.i.-/J^i.;o^i-..i.^6r>-.. <-,. ..ifc'lMiiSilwL.
FTOFOflAL K. k. WOOMRD RESEUO CORFOUTIC* laboratory ul Conoultlne Serrloa F. 0. Box ^05, Serndoo, Virginia
April 25, 196J
Br. Dos Feeler fro>rt Nui|ir
teed Indurtriee Aoeoelatlos 292 Madison Avenue So* Tori 17, Setr Tori
Deer Doctor Fouler,
Flret let at epologlu for tlx dolor 1 eeodlnf you a ronoreh propoaal to dlecueeed then you ololted trlth no loot ooth. I jut aelde tho notoo telco ot our conference for pro* poretlen of a proposal end they nn thoo alolald. After that,
tho thole proloot clipped froa ay Bind until your phono oall.
Wo aro oaeloolnc throe coploo of a proponed protocol for ranee fladlnc toxicity tecta on orgono-load ea^oundo.
The oot looted ooeto par coapound for eoodnetln< tho rufe flndlnf teoto ere ee follow.
Oral recce flndlnf Is rata Cornel ranee fInline Is rabblto yo Irritation
Inhalation, aoute
$ 15.00 75.00 05.00
115.00
Vo trill bo eesdlnc aloof a protocol for tho load Halt pelnt otudy at a later date.
Sincerely youra,
dlgnedj Oeoffrey Woodard
(Via*
Oeoffroy Woodard, lt.Ii Ihanaaoloflet
Boolean*
N 191.05
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V002ARD JC3SABCH CORPORATIOB
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ORAL-RATS; Rata velghlpf 1JO to 2J0 eraoe, faeted oweraltfit, vtlX V* given (loci* oral do*** of tb* compound under tcvt la a aultabla Tahlclo (ocm oil, polyethylen* (lyool JOO, Mtr, or water auepeaalca) a* follomi
2 2 2 2 2 Jl6 X
Vbtrt liii eultebl* po**r of 10. If DtHiau}, thla doaac* achedul# will b expanded la order to yield both eurrlTlpg and KB* urrlrlat aolemla. Sign* at ifcaraacaloclcal and toslcoloflool effect* *111 be recorded. Repreeentetlv* *unrl*ore aad poo-euiTleoa1* *111 be eubjected to pecropey, flurrlworm will be bold for Ik day* after do**e*.
tgRHAL-RABSlTBi Ibe fur *111 be reoared frea the tnsk of five young admit raoblte by cloee clipping. Tb* cc^ouad under teat either undiluted 1 a liquid or a auepeaaloo or aolutloo la a eultable vehicle *111 be applied to the tkla vltb geotla rubbing *lth a glaae rod until the coqxMad appear* to tart penetrated the akla or to be** beep epraad uniformly, lb* trunk vlll then be wrapped vltb a protective covering and tb* rabbit placed la It* caga. After 2k hour*, the ecrarlpg will be removed aad any axceaa ccagwaiad will be rcaowd from tb* ekla. Reading* for irritation (arythen*, edaao) *111 be aad* at tbet tla* end again at two, three, four, end eevea day*, dice* of ehamlcally Induced effect* will be recorded. At lk day* all turrlror* aad any aoo-eurvlvora la th* lat*rla vlll be eubjactad to n*cropey. Tb* doaag* eobedul* *111 be th* aea* *a for th* ret oral axoaptlng that oo* eplaal *t each doe* vlll be ueed aqd ao doe* over J160 mg/kg will be glean.
m nmiTATTOW-RABRITfli The eeterlal under teet will be placed la oo* eye of each of three rabbit*, th* other eye remaining u a coctroi, if a liquid, 0.1 ml *111 be ueed per eye. It a aolld, 100 e will be (round la an agate mortar *1U> 0.1 ml of polyethylene (lyool JOO ead the euepeneloo plaoed la th* eye. Reading* for erythema, *v*Uln(, ltitle, and oomeal damage vlll be aed* at 2, 2k, k8, 72 beam aad at 5 and 7 day* after application.
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w o c o muc nszuea itreined
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MVTt XWlUATMHi f*a r*t* or kltaroatlwoly fl** rat*, thr** (uloM tailIn alo* in 11 b plc4 la tultibl* tnb*l*tloo rhirrirr
lh ca*pouwl under ctudy mil b introduced *o *4 to yield practical taop6rlo l*rlj by urn of D*buU**r for r*latl*ly oao roletil* or bubbllac Orrlc* for rulettle oaapouad*. future t vculA cootlau* for (la to MTtn hour*. NMjurod aaount* of tb* total*tloo *tac*jfc*r would b nwn a **rr*l tlsn durtbd tb* cairn u.* tb *po*ur* for cbtalcel d*ti*ln*tloa of tb* Lml lm * tbo cb*db*r. *oo-*unrtvar *od *11 turwlwlac Ik d*y* would bo id>l*ct*d to a*crop*y with tpeeul ottoatloo twin* fives) to tb* Isx^i, 2 r*plx*tory p*tbv*y, linn, fclto*y*( *ad b**rt*.
Xd>*l*ttoo rtudi** would b* a*do only m*o tb* ea*f>oix>d *pp**r*d ~ to troooot poMlblo buard by tbl* rout* b*e*u* of It* pfayalo*!
prcgortl** or b*c*a* of high toxicity by other rout** of etelalctratlob.
Slpwdl OecffMy Oeoffrey Uoodord, ttodi April S3, J$63 "i-* Vc=.>-V - J-* `.*5 - V r-
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