Document yrvwBdvw9D7MvGQ4gapdLw6Q2
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonicacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was not noted. The testsample isFC-201AF. Currentinformationindicatesitisa mixtureof 3% PFOS, 18.59% diethyleneglycolbutylether,37.81% water,14% ethyleneglycol, 8.25% Acrylicfbamer,9% sodium octylsulfate0,.3% sodium laurylsulfate, 3% t(iethanolamine6,% sulfonatedalkylethersalts,and 0.05% tolyltdazole.
The followingsummary applies to a mixtum with incompletely charactedzed concentrations of impudties. Data may not accurately mflect the toxicityof the fluorochemical component of the testsample.
METHOD
Method: OECD Guideline209 Test type: Staticacute GLP: No Year Completed: 1995 Analyticalmonitoring: Dissolvedoxygen,temperature,and pH. Statisticamlethods: Resultswere determinedby calculatioonfthe% inhibitioinnrespiratiornate. Test organism source: Activatedsludge mixed liquorcollectedfrom the Metro Wastewater Treatment Plant,St.Paul,MN. Test condition:
Dilutionwater: MilliporMeilli-OTwmater Synthetic sewage: per OECD method Reference and testsolutionpreparation: A stock solutionofthe referencesubstance,3,5-dichlorophenowla,s prepared by dissolving 500 mg in1OmL 1N NAOH, dilutedto30mL withMilliporMeilli-QTM water,then broughttothe pointofincipienptrecipitatiwointh 1N H2SO4 and dilutedto IL withMilli-CiwTamter. The pH ofthe referencesolution was measured to be 7.3. Test substance was added directltyothe testvessels. Test vessels: Not given. Number of concentrations: one plusblank controli,nduplicateand 1 referencesubstance Temperature: 19.3- 20.7*C. Totalsuspended solids and pH on day of testing: 4.2 g/L (TSS in final1:.7g/L),initiaplH 6.0 and finalpH 6.5. Element Basis: Respirationinhibitioans determined by oxygen consumption.
Remarks:Uptakeratescorrectetdo200C forcalculations.
RESULTS
Nominal concentrations: Blankcontrolsand 1000 mg/L induplicateplus one referencesubstance at 10 mg/L.
Elementvalues: 30-minuteEC50=>1000mg/L 3-hour EC50 = >1000 mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapply tothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 3-hour EC5o foractivatedsludge respiratioinnhibitiownas determined tobe >1000 nig/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133
DATA QUALITY
ReliabilityK:limischranking= 2. This studymeets the criterifaorquality testing.However, sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number,N2964, 1995.
OTHER
Last changed: 6/26/00
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LJQUOR
LR Date Analyst
Page -Z--of -7
Type Test
ACTIVATED SLUD(3F; MIXED LLWOF3
Dale Obtained Mixed LiquorSuspended Solids(MLSS)
Datc, Used
LI,2
g/L Adjusted to
MLSS in finaltestsolution -7 g/L
InitipaHl LL@L (lsttestsoin.prepared)
FtnalpH
gIL (lastsoin.prepared)
Comments Aft,, I'l@@ll-
A
TEST SUBSTANCF-
2L -,@@4-jL@
Sample Description
InitipaHlof highestconc.tested le(,!
AdjustetdopH
Comments
REFERENCE STJBSTANCFSample Description InitiaplH, @of stock"solution c omments@@'@,@
I :POSURE es b ur'a'it'@on' DS etnpera r omm nt
mg/L) .c
26'
Adjustedto pH
ENVIRONMEUTAL
LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITCNTES.T.-209 TEST SOLUTIONS PREPARATION
Test Substance: Sample Description: 4,na@yst
@'C
Tc-st@-onc. mc,!L
mg added D@,r500 rnL
mL %iQ(l)waier opr 500 -L
mL syn. s@ewage fe,@,td>er500 -L
mL -icrobial
---------..-.-.-..
Total Volume r-L
MilliporeMilli-O high -punty.w-ater
ENVIRONMENTAL
LABORATORY
CECO ACTIVATED SLUDGE RESPIRATICN INHIORTICN
RAW DATA
R ;:t2C9
Tes, Substance:
A
Sourc:eof ActivatedSludge Mixed Liquor-
Co,,,eReceived: ql
Date Used:
30-NIINUTE EXPOSURE Test,'Substance C,,-,,,,-icentratioInL 0.5
EK',@@'@NTRC)L I
DLL,WLVF-D OXYGL-4 CONCENTRA-NCNS, mgl-
TIME-MINUTES
Temp
1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 OC
Li -2-
Li 7
BK CKDNTROL a
71 Li
s
3-HC)UR EXPOSURE
DISSOLVED OXYGEN CONCeM:7ATIONS, mgt
TestSubstance C o n c,e n tration
TIME - MINUTES
Temp
0 5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
cc
SK CCNTROL I.
Li,5 Ll C,
5@q Li,clH.q
-3 ,
F3KCONTROL [I v ;,nil
2-, 71cl
-activtaed.@slijdgq xed liquor*.,-,Initial
al@
14,.@ofhigheii@66nc-6ntr@'@b'@!tested@- Initial
o
51 1--/,
....
ENVIRONMENTAI- LABORATOrlY OECD ACTIVAIED BLUDGE RESP!FiAi-iC)N CALCULATION OF RESULTS
Lr:
Date:
Test Substance: FC-201AF
Analyst:Qw
30.Mln te ExDosure mq/L 0.5 1 0
Dissolvpd 1.5 2.0 g 2,..556 301..0090
n o eennttrarlaotnsiS@m M 5.5 T,-p,-C R (1) R a (2)
@E3Kl
7.0 6.6 6.3 6.0 5.6 5.3 5.0 @4C47..0701 4.4 4.1 3.7
i 1000.0 4.2 3.4 2.8 2.2 1.5 0.9
---
Dup.
0 3.3
2.2 1.5 1.0
19.9 20.7 20.6
0.6364 1.2600 1.1800
0.6408 1.2001 1.1318
---
BK2
8.0 7.6
0'/d0iffin controls (4):
7.4
10.2
3-Hour Ex osure
3.5
1.0
1 .5
-@K 1 7.7
7.
7.2
iooo.o 5 @ 1
4.5
4.0
DUE.
--- B
5.4
7.2 6.9
@6 6.3 1 6.1 5.8 5.4
EC50 (95% Cl): I'@ , , ('( ' (@ 1L,
Dissolv d 0- en Concentrati-
i 2.. 1 2.5
3.0
3.5
4.0 --4.5
5.0
6.9
6.7
6@4
6.1
5.8
5.5
5.3
3.6
3.0
2.6
2.0
1.6
1.0
0.5
4.9
4.4
3.9 1 3 4
3.0
2.5- 2.0
19-8
0.5709 10.5789 RaCm (5)
5.5
'C- R (1) Ra (2)
4.9
19.4
0.5552 0.578E
20.3
1.0000 0.9794
1.5
19.9
0.9600 0.966o
--@K2
8.3
8.0@7
C
7,5
7.2
7.0
6.7
6.4
6.1
5.8
5.5
diffin controls (4): 1.6
ECSO (95% Cl):
19,3
-0.-5o6o
0.587 RaCm (5
a@IJUSI1E0d20'C.(FtlEoXI % intiibtijcwl@@-RaCm) FI&S/(RaCm)1100 @4) % ditlefoncotncontrols ([Rarlkl.RaBk2l/R&CfrllOO(Two contr@Asmust be *Ohin 15% co each o(her.) Mean adjusted R, oDntro4s-[RaBk I-RaBk2V2
1072
tracm. meanadiuvodR.coc*ois I;Bk. blankcontrolS,- Sam*
itobs- sampietamp i l@x-,-Totm-2@O'C
ENVIRNOMENTAL
LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBMN TEST SOLUTIONS PREPARATION
LRTEST#209
Test Substance:
Sample Description:
Analyst:
Date:
Z,
Stock Solution Preparation: r.. IL
1 (7,
@,A L 7bC
Ke
41
Let
c,L4 I
"Tc
7@
Test Conc. ma/L
mL added per 500 mi
ML MQ(l) water 22r 500 mL2- @@4
Z7
mL syrlsewage feed Def 500 mL
mL microbial inoculum per 500 rrL
7z c@ C,
oc@
TotalVolume -nL @7c@ c,
Islip,
LR
ENVIRONMENTAL
LABORATORY
OECD ACTIVATED SLIJDGE RESPIRATICN INF.ISITICN T=-ST -2,39
RA'.';CATA
Test Substance:
Source of Ac,,ivatedSludge Mixed Liquor: Date ;@eceived: Anal,yst:
Date LISEdi
c- 7
30@-tAINUTE EXPOSURE
DIL@'WLVE-D OXYGL-4 CCNCr--NTr-tATICNS, mgL
Te7@t-c,3ubsl,ance
IME-MINUTES
Ccr,ctantration /00 .5 1.0 1.5 2.0 2.5 3.0 S3-5L4-0
Temp 4.5 5.0 5.5 CIC
E K C@C NT-H CJ- I
-7.
-7,2 -7 L
7
6K CCNTROL 11
3-HOUR EXPOSURE
Test Substance
Concentration IL) 0.5 F-KCONTROL I
-77-7
DISSOLVED OXYGE@4 CCNCE-NTRATICNS., mg(L
TIME - MINUTES
Temp
1.5 2.0 2-5 3.0 1 3.5 4.0 4.5 5.0 5.5 cc
6-,
CON'MOLU
7@'
neszcon cenir,Auc
e arfu'
C4
W-;Ilquo tette
lnilid
@O
ildi
-@Final n at
ERNIRC)NMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION IN@IIBITIONTEST 0201)
A 1 I- 1 1LAT!ON CF RESliLTS
Test Substance: 3.5-DICHLQEQMENOL
Analyst.QW
30-Mln te @XE sure lrl)q/L 0.5 1.0
7 0 6.6
,Ei8Eo@, 7@8
1.5 2.0 6.3-- 6.0
Dissolv@d 0 en Concentrationsm, /L
3 0 3.. 4,0 4.5 5.0 5.5
M 'L 5--5
5,6 5.3 5.0 4.7 4.4
7
7.7 7,5 =77.2@ 7.0 6.8 6.66-
6.1 5.9
L.R N296 Date: __-2LL'
Tamp,*C R (1)- Ra 19.9 0.6364 0.64 20.3 --0.4339 0.42
BK2
o@7
7.-4-
% ditfincontrols(4): 10.2
3-liourExposure ifnq/L 1 0,5 1.0
BK1 7.7 7.4
10 B-3 8.2
7.2 6.9 8 1 .8.0
6.6 6.3 6.1 5.8 .5.4 5.0
Dissolvd Oxy on Concentrationsm,a/L 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.7 6.4 6.1 5.8 5.5 5.3 4.9 7.9 7.7 7.6 7.4 7.3 7.1 7.0
19.8
Tomp, *C
19.4 20.4
6@5709 10.57 RaCm
R (1)__ Ra 0.5552 0.57 0,2776 0.27
B ta
8 0 7 8 7 5 7@2 1 7.0 6.7 1 6.441@ 66@-l1 5.8 5.5 19.3 0.5600 0.58
diffir)control(s4): 1.6
RaCm
(i)fi.(-).Yuor,ui)iakerate (mg 0,'l @in)
lo- 1072
i
(2) f@am fi adjust&J lo 20'(' iRo I xl
alitist@d u)(Arols
(3) %
Fla',.,(Rac@@1100
'Elk-blank conlrolS,- s-aM pie
(4)% d,tfere@a inc-,Ifols [IfisfikRi&EI@21fla'@111,0 (T.o co,il,,I@s,)slt)e@dh,n 15% ofearh (,It@at
(S) Mean sdl,,slwiR
IRA[Ikl,RaBk2@2
Tobs. samp4a lamp X.- Tobs.20'G-
Chain of Custody Record
3M EnvironryientalLaboratory
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preservatives L0)
Date Time
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Time
Date
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