Document yrp14QrrbpR7Dk53eJyO4kDXD

3nduA3Ual BI O * T E S T JlaSMa&'ueA-, 3m. 1610 FRONTAGE ROAD NORTHBROOK, ILLINOIS 60062 REPORT TO MONSANTO COMPANY TOXICITY, REPRODUCTION AND RESIDUE STUDY WITH AROCLOR 1242, LOT ifAR-255 IN WHITE LEGHORN CHICKENS JUNE 15, 1971 IBT NO, J8746 DSW 035758 STLCOPCB4019583 Sn&uAfoial BIO -TEST 1610 FRONTAGE ROAD NORTHBROOK, ILLINOIS 60062 3m. June 15, 1971 Mr. Elmer P. Wheeler Manager, Environmental Health Monsanto Company 800 North Lindbergh Boulevard St. Louis, Missouri 63166 Dear Mr. Wheeler: Re: IBT No. J8746 - Toxicity, Reproduction and Residue Study with Aroclor 1242, Lot No. AK-255 in White Leghorn Chickens We are submitting herewith our laboratory report dated June 15, 1971, prepared in connection with the above study. Very truly yours, JCC/kjl J. C. Calandra Pre sidcnt DSW 035759 STLCOPCB4019584 Htuhtii'iial B I O - T E S T Jlalto-uda'iicS, 9kc. REPORT TO MONSANTO COMPANY TOXICITY, REPRODUCTION AND RESIDUE STUDY WITH AROCLOR 1242, LOT #AI<-255 IN WHITE LEGHORN CHICKENS ' JUNE 15, 1971 IB T NO. J8746 I, Introduction At the request of Monsanto Company, a toxicity, reproduction and residue study with Aroclor 1242, Lot //AK-255 in white Leghorn chickens was initiated. This report presents the results of the investigation conducted at Wedge's Creek Research Farm Division, Industrial BIO-TEST Laboratories, Inc. 035760 STLCOPCB4019585 94i<Xiitial B i O - T E S T laJwiato-xici, 9<-tc. 2 II. Summary A toxicity, reproduction and residue study was concluded with Aroclor 1242 in white Leghorn chickens. Dosage levels were 2, 4 or 8 ppm mixed in the diet. Eggs were collected for production, quality, physical characteristics and hatchability. Eggs also were collected for residue analyses. The chickens were observed for toxic effects and their tissues were saved for analysis at the end of tire hatch data, as well as after a 30-day (recovery) period during which time they were fed control diet. There were no effects on the chickens as judged by body weight, food consumption, behavioral reactions and pathology. There were no effects on egg production, quality (candled), specific gravity or egg shell thickness. Although the percent of fertile eggs in all groups was about the same, there was slightly lower percent hatch DSW 035761 STLCOPCB4019586 jWveilW/ B I O - T E S T jEaltaiaianici, 9nc. 3 in (be T-II (4 ppm) group and in the T-1II (8 ppm) group than (he per cent hatch in the control group. All chicks that, hatched were normal. Respectfully submitted, INDUSTRIAL BIO-TEST LABORATORIES, INC. Report prepared by: S^cJUL fMlu'V Dale Fletcher, B.S. Group Leader Wedge's Creek Research Farm Report approved by: "DorTald IT0 Jenkins, D,, V. M. Manager and Technical Director Wedge's Creek Research Farm J unc 15, 19 71 al :db Otis E. Fancher, Ph, D. Scientific Director DSW 035762 STLCOPCB4019587 BIO-TEST JlaJfolaJiX'uci, 9nc. 4 III. Proced re A. Experimental Animals The animals employed in this study were white Leghorn chickens. A total of 80 hens and 16 roosters were used. B. Organization of Groups The 96 chickens were equally divided into one control group (C) and three test groups (T-I, T-II and T-III), each consisting of four males and twenty females. A structural outline of the experiment is presented in Table I. DSW 035763 STLCOPCB4019588 StuLuinial B I O - T E S T lab&'iaivu&L, 9nc. 5 TABLE I TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Outline of Experiment Group Control T-I Number of Animals Males F emales 4 20 4 20 Dose Levels TestMaterial .. .. (PPm) None Aroclor 1242 Lot #AK-25 5 2 T-II 4 20 Aroclor 1242 Lot ft AK - 2 5 5 4 T-III 4 20 Aroclor 1242 Lot #AK-255 8 DSW 035764 STLCOPCB4019589 Dnduitnial B l O - T E S T lahyiattyiie-t, $rtc. 6 C. Dosage and Feeding All groups received the same base feed-. Test diets were prepared weekly. The test materials were diluted with acetone before addition to the feed to assure even distribution throughout the ration. An equal amount of acetone, without test material, was added to the control feed and the feed was then blended in the same manner as the test feeds. All animals were allowed water and their respective control or test feed ad_ libitum. Dosing began when the birds were 12 weeks of age and continued until hatching results were available. D. Observations Observations were made daily to ascertain the presence or absence of clinical'signs of toxicity indicative of test material effect. All chickens were individually weighed and the group feed consumption determined weekly. As each group began laying, the eggs were collected daily and the egg weights recorded. When the egg weights were consistently between 50 and 60 grams, and at least 50 percent of the hens in the group were laying, egg collection for that group was started. The time required to meet these criteria was recorded for each group. DSW 035765 * Purina Flock Chow, Ralston Purina Co. , St. Louis, Missouri. STLCOPCB4019590 hubuiiUil B I O - T E S T JlalMMaiaiicA; Unc. 1 The egg production was collected for the following studies: 1, Egg Production and Quality Eggs were collected, weighed and candled for a number of seven-day periods. The total number, the individual weights of eggs collected and the total number of uncracked and umblemished eggs were recorded. 2. Hatchability ' One-half of all non-defective eggs were randomly selected for hatching. These eggs were turned twice daily during the seven-day collection period, then placed in an incubator maintained at 99 to 100 F, with a wet bulb reading of 85. This procedure was followed until a minimum of 100 eggs from the control group and each test group had been set for hatching*. The following was recorded: a. Number hatched b. Number unhatched c. Viability of chicks over a 30-day period during which feed and water were permitted ad libitum. d. Examination of unhatched eggs for fertility and stage of development. e. Weight of chicks after Day 1 and Day 30. DSW 035766 * Ninety-three eggs set for hatching on T-I level due to poor egg production and quality. STLCOPCB4019591 SaJ-itiUkd B 1 O - T E S T labaiatawA-, Due. 8 3. Physical Characteristics of Eggs One-fourth of the non-defective eggs and all of the defective eggs were examined to determine the following: a. Specific gravity b. Thickness of shells at equatorial circumference (three measurements/egg). 4. Residue Studies The remaining one-fourth of the non-defective eggs were quick-frozen and shipped to Monsanto Company for residue analysis (shells and frozen contents separated). After hatching results were available, one-half of the chickens in each group were sacrificed, defeathered and subjected to a gross pathological examination. Pooled samples of muscle, liver, fat and kidney from each group were collected and shipped to Monsanto Company for analysis. Pooled samples of feathers from each group were also shipped to Monsanto Company for analysis. The remaining chickens were fed the base diet without test material for 30 days. The eggs were collected, weighed and candled during the recovery period. Ten eggs (minimum) per group per week were quick-frozen and shipped to Monsanto Company for analysis. At the end of the recovery regimen, the remaining chickens were sacrificed and handled in the same manner as at the end of the test feeding period. OSW 03576? STLCOPCB4019592 Sndiiii'uo.l B i O - T E $ T 9<ic. 9 IV. Results A. Parental Generation 1. Body Weight Data The average male and female body weights during the test diet period are shown in Tables II, III, IV and V. The average male and female body weights during the recovery period are shown in Table VI. These data are summarized in Table VII, No significant differences in body weights were noted. DSN 035768 STLCOPCB4019593 Bi O OSW 0 3 5 7 6 9 Sex M F Week : 0 I, 31 I. 03 TABLE II TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Test Diet Period Mean Body Weight Data (kilograms) Control Group 1 2 345678 1. 37 1.38 1. 49 1. 48 1. 59 1.65 1.66 1.68 1.09 1.13 1. 21 1.22 1. 34 1.41 1.43 1.41 9 1. 57 1. 35 Sex M F Week.: 10 1.48 1.40 11 1.69 1.41 12 1.79 1.45 13 1. 86 1. 52 14 1. 87 1.43 15 1.96 1. 52 16 1.90 . 1. 54 17 1.97 1.65 18 1.95 1. 55 19 2. 01 1.60 Sex M F Week : 20 2. 02 1.55 21 2.07 1.68 22 2. 10 1.69 23 2. 10 1.70 24 2. 12 1.69 25 2. 15 1.70 26 2. 17 1.75 27 2. 20 1.77 28 2, 22 1. 76 29 2.21 1.75 Sex M F Week : 30 2. 22 1.75 31 2. 26 1.74 32 2. 31 1.75 33 2. 33 1. 76 34 2. 36 1.77 35 2. 35 1.78 -1 in tr> 'V -('ai o J'h u lu iiA ia l B I O - T E S T J a k n a jw ie A , 9nc. OSW 0 3 5 7 7 0 Sex M F Week: 0 1. 32 1.03 TABLE III TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Test Diet Period Mean Body Weight Data (kilograms) Test Group I 1 2 34 56 7 8 1.40 1.49 1. 57 1. 59 1.64 1.74 1. 75 1.78 1. 13 1.19 1. 26 1.29 1. 36 1.43 1.43 1.41 9 1.84 1.49 Sex We ek: 10 M 1.77 F. 1.44 11 1.75 1.44 12 1.76 1.42 13 1. 82 1. 51 14 1. 76 1. 53 15 1.92 1. 59 16 1.97 1.58 17 2. 01 1.61 18 2. 03 1. 60 19 2. 03 1.62 Sex M F Sex M F Week: 20 2. 04 1.60 Week: 30 2. 25 1.69 21 2.09 1.65 31 2.27 1.69 22 2. 09 1.64 32 2. 27 1. 70 23 2. 14 1.65 33 2. 24 1.69 24 2. 18 1.67 34 2. 17 1. 72 25 2. 16 1.67 35 2. 33 1.73 26 2. 21 1.68 27 2. 24 1. 70 28 2. 27 1.69 29 2. 28 1.72 1 S 3 1 O I 9' Sex M F Week: 0 1.33 1.08 TABLE IV TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens T ' Diet Period Mean Body Weight Data (kilograms) Test Group II 1 2 345678 1.40 1.45 1. 54 1. 56 1,61 1.66 1.70 1. 65 1.17 1.22 1. 31 1. 37 1.45 1.48 1.45 1. 36 9 1.67 1.46 Sex M F We ek: 10 1.65 1.41 11 1.65 1.40 12 1.69 1.43 13 1. 85 1.47 14 1.93 1. 50 15 2. 01 1. 57 16 2. 08 1.60 17 2. 14 1.63 18 2. 18 1.61 19 2. 18 1.65 Sex M F Week: 20 2. 19 1.63 21 2. 26 1.71 22 2. 29 1.72 23 2. 33 1. 73 24 2. 38 1. 77 25 2. 35 1.73 26 2. 38 1.76 27 2. 41 1.74 28 2. 45 1.75 29 2.45 1.77 DSW 0 3 5 7 7 1 Sex Week: 30 31 32 33 34 35 O 0 M 2. 19 2. 30 2. 30 2. 29 2. 31 2. 33 O DO oU F 1.71 1.74 1.78 1.79 1.79 1.79 1 S3 1 " O I 8 Sex M. F Week: 0 1. 32 1.04 TABLE V TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction &: Residue Study - White Leghorn Chickens Test Diet Period Mean Body Weight Data (kilograms) Test Group III 1 2 3456 78 1.42 1.45 1. 56 1. 57 1.68 1.72 1.75 1.75 1.13 1. 16 1.24 1. 27 1. 35 1.39 1.41 1.40 9 1. 82 1.44 Sex M F Week: 10 1.69 1.39 11 1.87 1.40 12 1. 89 1. 39 13 1.97 1.50 14 2. 07 1.48 15 2. 10 1.51 16 2. 13 1.53 17 2. 21 1. 54 18 2. 24 1. 55 19 2. 24 1. 56 DSW 0 3 5 7 7 2 Sex M F We ek: 20 2. 28 1. 55 21 2, 30 1. 59 22 2. 38 1.61 23 2.42 1.62 24 2. 38 1.61 25 2. 38 1.61 26 2.44 1.66 27 2. 45 1.65 28 2. 45 1.65 29 2. 52 1.67 Sex M F Week: 30 2.40 1.60 31 2. 45 1.67 32 2. 40 1.65 33 2.49 1.69 34 2. 40 1.70 35 2. 32 1.68 * STLCOPCB4019598 Group Control Group T-I Group T -II Group T-III TABLE VI TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Recovery Period Mean Body Weight Data (kilograms) Sex M F Week: 36 2. 42 1.72 37 2.46 ' 1.76 38 2. 45 1.75 39 2.48 1.77 40 2. 50 Sacrifice 2. 50 1.78 Sex M F Week: 36 2. 43 1. 82 37 2. 51 1.83 38 2. 51 1.82 39 2. 52 1.85 40 2. 52 1.71 Sacrifice 2. 52 1.71 Sex M F W e ek: 36 2. 36 1. 85 37 2. 38 1.84 38 2. 39 1. 85 39 2. 37 1.85 40 2. 40 1. 85 Sacrifice 2.40 1. 89 Sex M F Week: 36 2. 41 1.60 37 2. 49 1.62 38 2. 52 1.67 39 2. 51 1.61 40 2. 59 1.61 Sacrifice 2. 59 1.61 >-** -vj 00 1 S 3 1 - 190 4^ is 3 i-o !s Group C T-I T-II T -III TABLE VII TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Summary of Mean Body Weight Data (kilograms) ODay: Male F emale Test Period Male Female at Sacrifice Male F emale 1. 31 1. 03 _ 1.92 1. 55 2. 50 1.78 1. 32 1. 03 1.96 1. 54 2. 52 1.71 1.33 1.08 2.02 1. 58 2. 40 1. 89 1.32 1.04 2. 10 1. 51 2. 59 1.61 o O u> vn *xj XJ f- cn STLCOPCB4019599 DtiJmUiai B l O * T E S T JlaltonatMieA-, 16 2. Food Consumption The food consumption data are presented in Table VIII. The food consumption was considered to be normal with no significant differences among groups. DSrt 035775 STLCOPCB4019600 Dtultiii'Ual BIO-TEST J.aLo.'iaio-niei, Dno. TABLE VIII TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Average Food Consumption (Grams/Bird/Day) Week C Group: T-I T- II T-III Test Period I 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 . 23 24 25 26 27 28 29 30 31 32 33 34 35 36 77 68 76 75 103 91 67 99 82 115 84 105 105 100 156 84 114 113 101 107 163 151 184 105 142 123 180 128 114 113 129 125 115 116 98 194 107 62 65 71 74 88 82 119 94 107 93 99 130 101 147 90 104 118 119 106 140 121 143 93 123 123 168 126 136 119 124 108 103 93 119 1 55 107 83 68 74 92 98 80 98 114 106 76 109 106 90 191 118 128 125 114 120 124 134 195 79 111 125 163 118 113 103 137 132 134 121 97 179 77 92 80 68 90 88 78 96 107 102 92 109 110 101 176 105 119 108 99 106 112 106 159 66 1 50 183 183 118 130 129 144 116 148 122 111 145 DSW 0 3 5 7 7 6 STLCOPCB4019601 tyudjidnial B l O - T E S T Jalf&'iai&'uM, 9nc. 18 TABLE VIII continued TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Average Food Consumption (Crams/Bird/Day) Week Group: C T-I T-II Recovery Period T - III 37 144 174 209 130 38 134 147 202 182 39 136 149 152 177 40 136 134 138 117 41* 136 134 138 117 >!' One day DSW 035777 STLCOPCB4019602 9nduitnial B I O - T E S T lakocaio-'iiei, 9tic. 19 3. Mortality and Reactions There were no untoward behavioral reactions or mortal ities attributable to the test material. Mortalities were considered normal and were due principally to the avian leukosis complex (lympho matosis). The parental generation mortality, chronology and clinical diagnoses are presented in Table IX, " The mortality data are presented in Table X. DSW 035778 STLCOPCB4019603 hiduitual B I O * T E S T Jal&icdtVutA. Di-ic. 20 TABLE IX TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Mortality, Chronology and Diagnoses Group Animal No. Sex Week Died Clinical Diagnoses c 1611 F 9 Egg ruptured 1601 F 14 Lymphomatosis 1615 F 15 1616 F 16 Egg ruptured Ruptured intestine 1607 F 19 Marek's Disease 1605 F 20 Cystic oviduct 1613 F 29 Lymphomatosis 1606 F 32 Lymphomatosis T-I 1628 1585 1631 1622 1515' F F F F M 10 23 31 33 34 Injury Unknown Injury Lymphomatosis Lym ph o mato sis T-II 1639 1654 1 524 1657 1650 1648 1516 F9 F 10 M 13 F 15 F 16 F 20 M 29 Egg ruptured Lymphomatosis Cannibal i zed Lymphomatosis Lymphomatosis Marelds Disease Unknown T-III 1671 F 9 1529 M 10 1674 F 18 Lymphomatosis Lymphomatosis Lymphomatosis DSW 035779 STLCOPCB4019604 Hndidt'iial B t O - T E S T laJus'iata'iioi, dr.c. 21 TABLE X TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Mortality Data Group Number Tes ted MF Number Died MF Total Dead Total Tested Percent Dead C 4 20 0 8 8/24 . 33, 3 T -I 4 20 1 4 4/24 20. 8 T-II 4 20 2 5 7/24 29. 1 T-III 4 20 1 2 3/24 12. 5 OSW 035780 STLCOPCB4019605 DtuhtsUial B ! O - T E S T l.abo'iaiyuel, 9nc. 22 4. Gross Pathology Postmortem examination of all birds revealed no gross pathological tissue alterations attributable to effects of the test material. DSW 035781 STLCOPCB4019606 !)ttdu-d'Ual B l O T E S T JaltfrialA'u&i-, 9mc. 23 B. Eg_gs 1. Egg Production and Quality The weekly egg production data are presented in Table XI. The production of Test Group I (T-I) was lower than that of other groups. The egg quality data are presented in Table XII. DSW 035782 STLCOPCB4019607 .W W W B IO -TE S T STLCOPCB4019608 TABLE XI TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Egg Production Data Week Control Average Weight Percent (grams) Production/Day 5 33. 0 6 37. 3 7 39. 8 8 41. 7 9 42. 5 10 42. 3 11 44. 4 12 46. 2 13 46. 5 14 48.4 15 48. 9 16 49. 5 17 50.7 18 50. 9 19 51.7 20 51.6 21 52. 4 22 53. 3 23 54. 9 24 54. 1 10. 0 26.4 40.7 37.1 45.9 48.9 51.1 60.9 57. 3 61.1 56.3 74. 1 60.7 49. 1 67.6 59.6 63. 3 67. 3 61.2 55.T T-I Average . Weight Percent (grams) Production/Day Average Weight (grams) T-II Percent Production/Day 32. 1 41.0 43.0 43. 6 43. 6 45. 1 45. 0 46. 8 47. 5 48. 8 48. 4 48. 9 50. 5 50. 5 51.6 51.6 52. 5 53. 3 56.0 54. 9 Test Period 7. 1 31.4 22. 8 43. 5 45. 7 35. 0 39. 8 36. 0 52. 6 54. 8 61.6 51. 1 64. 6 60. 1 53. 3 50. 3 54. 8 48. 8 39. 6 40. 0 39.4 40. 9 42. 8 42. 0 43. 5 43. 1 45. 2 46. 4 46. 5 48. 5 49. 3 49. 3 50. 4 50. 7 50. 4 51. 1 52. 0 52. 7 53. 1 55. 0 45. 7 60. 7 59. 2 65. 7 60. 0 59. 5 65. 8 73. 8 65. 8 64.2 63.8 73. 2 75. 8 69.6 68.7 71.4 71.4 74.2 58. 0 62. 8 T-III ^ Average Weight Percent g (grams) Production/Dayl'' iS. 37. 2 39. 2 41.6 43. 2 42.9 44. 0 44. 6 46. 9 47. 6 49. 7 49. 0 49. 8 49.7 50. 5 49.9 52. 3 53. 3 53. 3 54. 3 53. 1 16. 4 32.9 47. 6 46.4 48. 6 47. 4 61.7 62.4 60. 2 56. 4 60. 9 63. 2 61.2 65. 1 67. 5 58. 7 55. 6 56. 3 56. 3 55. 5 OSW 035783 I  lih ia l B I O - T E S T laluyiatoA ic-l, Du&. STLCOPCB4019609 TABLE XI continued TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction &r Residue Study - White Leghorn Chickens Egg Production Data . Week Control Average Weight Percent (grams) Production/Day 25 55.7 26 54.9 27 54.4 28 54. 5 29 54.6 30 54. 8 31 54. 3 32 54. 5 33 56. 3 34 55. 5 35 55. 8 54. 1 53. 1 45. 9 53. 1 41.9 49. 5 48.4 . 44.9 - 42.9 38. 1 39. 3 D (n 36 37 38 39 40* 54.4 o U' 26.2 55.9 . 33. 3 56.0 f' 33. 3 56. 5 26.2 57. 1 16.7 Average Weight (grams) T-I Percent Production/Day T-II Average Weight Percent (grams) Production/Day 56.2 56.2 55.6 55.7 55. 5 55.6 56. 0 56.6 57. 1 55.7 54.7 Test Period continued 39.6 43.6 28. 5 31.7 23. 8 33. 3 32. 7 20. 1 16.8 27.6 35. 7 55. 8 54. 8 55. 2 54. 7 55. 3 55. 8 55.6 56. 0 57.6 56. 8 ' 56. 8 64. 7 60. 0 61.9 60.9 57. 1 56. 1 53. 3 55.2 48. 5 51.4 43. 8 Recovery Period . 57. 3 57. 4 49. 7 60. 1 0 30. 1 39.6 46.0 49.2 0 , 56. 1 56. 9 56. 5 59. 1 57.6 50.0 44. 6 39. 2 32. 1 20. 0 T-III Average Weight Percent (grams) Production/Da 56. 3 56. 1 56. 7 55. 0 55. 7 54. 5 57. 0 57. 3 59. 5 57. 3 57. 1 51.6 42. 8 51.6 47. 6 42. 1 38. 9 36. 5 37. 6 38. 8 31.7 34. 1 50. 9 60. 4 55. 1 58. 2 0 50. 8 39.4 51.6 26. 9 0 * One day tytulH-it'Ual r ! O - T E S T J.abo'tato'i-U'.'l, 9nc. 26 TABLE XII TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Egg Quality Data Group Defective Eggs Number Percent Normal Eggs Number Percent Total Number C 292 52, 9 260 47. 1 552 T-I 251 54. 8 178 45. 2 458 T-II 211 49. 5 215 50. 5 426 T-III 226 53. 4 197 46. 6 423 DSW 035785 STLCOPCB4019610 $rtdn<tiual B l O - T E S T /a}ioA<ijo*i<eA., 9*tc. 27 2. Hatch ability The hatchability data are presented in Table XIII. The percent of eggs hatched was significantly higher in the control group than in the test groups.. The percent of hatch was slightly low in the T-II group and quite low in the T-III group. DSW 035786 STLCOPCB4019611 SiijJu-iiual B l O - T E f> T PnJtM/iiMipA, 9itc. 29 3. Specific Gravity The specific gravity data of the eggs collected and candled are presented in Table XIV. There were no significant differences in specific gravity among the groups. DSW 035788 STLCOPCB4019613 vtyw fo f i s 3 i - 0 I 9 rW 'T 't TABLE XIV TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction &: Residue Study - White Leghorn Chickens Specific Gravity Data Normal Eggs Defective Eggs Total Eggs Group______ Number Average . Specific Gravity_______ Number Average Specific Gravity_______ Number Average Specific Gravity c T-I T-II T-III 260 178 215 197 1.081 1. 090 1. 082 1.075 292 1.078 251 1.077 211 1.082 226 1.080 552 1.080 458 1. 083 426 1.082 423 1. 077 ot/0 OOJ Ul J 03 U> o STLCOPCB4019614 HnAuAinjal B 1 O - T E S T Jlab&uzt&u&l, 9*vz- 31 4. Egg Shell Thickness The shell thickness data are presented in Table XV. The shell thickness was about the same for all groups including the control group. DSW 035790 STLCOPCB4019615 Q- ipf; 'Y&n-'CtDkWfDX g <; 3 f - o i a r w ' T ' c Group C T-I T-II T -III TABLE XV TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Egg Shell Thickness (inches) Normal Eggs Average Number Thickness (10-^) Defective Eggs Average Number Thickness (10-^) Total Eggs Average Number Thickness (10-^) 90 12. 0 84 12. 0 94 13. 0 98 12. 4 201 246 211 225 12.0 12. 0 12. 0 12.4 291 424 305 323 12. 0 12. 0 12. 0 12.4 DSW 0 3 5 7 9 1 u> (\J fridti-ihial B I O - T E S T J.a-lM-'ial&'iUi, 9+ic. 33 C. Fj Generation 1. Mean Body Weights and Viability The mean body weights and viability data are presented in Table XVII. There were no significant differences between groups in either the Day 1 or Day 30 mean body weights. The percent viability was slightly lower in the treated groups than it was in the control group, but the differences were not significant or reduced in a dose related manner. Z. Gross Pathology Postmortem examination of all F] chicks dying on test and those sacrificed at 30 days revealed no pathological tissue altera tions. DSW 035792 STLCOPCB4019617 D ^ d m a l B IO -TE S T J e DSW Q 3 5 7 9 3 TABLE XVII TEST MATERIAL: Aroclor 1242 Toxicity, Reproduction & Residue Study - White Leghorn Chickens Mean Body Weight and Viability Data Group Number of Chicks Day 1 Mean Body Weight Day 1 (grams) Number of Chicks Day 30 Mean Body Weight Day 30 (grams) Percent Viability C T-I T-II T-III 43 37 35 15 36. 5 36. 0 35. 5 35. 7 40 29 27 12 261.9 255. 0 242. 1 245. 8 93. 2 78.4 77.0 80. 0 w