Document yrdDYpQ2mjGvxdzLQwnDJ1BjE

BACK TO MAIN Study T i t l o Acute T o x i c i t y of N2803-4 t o tho Fathead XIIinOW, Phephdlerr prcreld8 Data Ooquiroxmntr Ouidolfne Uurber V.8. tPA-TSCA, Guideline 797.1.400 Aut tior 8 Timothy J. Hard Peter L. Kowalaki Robert L . Boeri Study Canplated November :21, 1995 sponrlor 3n Com,pany Environmental Laboratory 935 Bush Avenue Building :I-3e-09 St. Paul, ninnoeota 55144 T.R. Wilbury Labciratoriee, Inc., 40 Doaks Lane Marblehead, )Uoaac:hueetts 019451 T . R . n i l b u r y s t u d y Number 894-TH BACK TO MAIN Thin 8tudy wan conductod 8ccording t o EPA Good Labo:mtory P r a c t i c e Rogulatfonm ( 4 0 C I R 7 9 2 ) , w i t h t h o f o l l o w i n g o x c o p t i o n . Tho B t a b i l f t y of t h o t e a t nubntance wae anmumad but n o t v o r i f i o d a t T.R. Wilbury Laboratorion. Doviationn from t h e atudy protocol and protocol .mmdnunta a r o promontod in s o c t i o n X I 1 of t h i 8 r o p o r t . Study Director T.R. Wilbury study Number 894-TH BACK TO MAIN . ._... - .-- .... .-..- . . .. . Submittod by: T.R. wilbury Laboratories, Inc. 40 voak. Lane Marblehead, Mam#achusetts Certification: T.R. Wilbury Laboratories, Inc. study number 894-TH, Acute Toxicity of N2803-4 to the1 Fathead Minnow, Pfmephalas promdas, wan auditod by the Quality ASBUranCe Unit for compliance with the protocol, etandard operating procedure., and applicable Good Laboratory Practicoa (U.S. EPA, 1993). Quality assurance audits were performed and the findings reported to T.R. Wilbury Laboratoriea management and the study director on the following dates: Aspect Audited Protocol: In-life: Draft Reportr Final Reportr Audit Date e/ 4/!35 9/26/!)5 10/30/!)5 11/21/!)5 Reportod to study Director a / 4/9'5 9/26/9'5 10/30/9!5 11/21/9!5 Reported to Management a / 4/95 11/2 1/95 11/21/95 11/21/95 Af'thur P. Paradice Quality Assurance Officer T.R. ililbury Study Number 894-TH -Page 3 of 22 BACK TO MAIN I 11. TAELZ OF CO-6 SECTION: I. Good Laboratory Practice Compliance statement 11. Quality Assurance statement 111. Table of Contents IV. Index of Tables and Pigureo V. Sumnary VI. General Information VII. Introduction VIII. nethods and naterials IX. Reaults X. References XI. signature Page XII. Protocol Amendmnts and Deviations Appendix A. Hater Quality Data from Toxicity Teat PACE 2 3 4 5 6 7 8 8 13 17 18 19 20 T.1. Wilbury study Number 894-TH Page 4 of 22 BACK TO MAIN Table 1. Table 2. chemical characterization of a representative sample of dilution water usetd for the toxicity test with fathead minnows, P.hephalespromelas, and N2803-4 Test system summary for the toxicity test with N2803-4 and fathead minnows, Pimephales promelas Table 3. survival and nublethal effect data from the toxicity teat with fathead m:tnnows, Pimephales promeha, and N2803-4 Table 4. median lethal concentrations (LCSOS) from the toxicity test with fathead minnows, Pimephales promelas, and N2803-4 Table A.1. Dimsolved oxygen concentration, conductivity, PR, and temperature measured during the toxicity test with fathead minnows, Pimephales promelas, and N2803-4 Figure 1. survival of fathead minnows, Pimephales promelas, exposed to N2803-4 for 96 hours PAGE 9 11 14 16 21 15 T.R. Wilbury study Number 894-TH Paye 5 of 22 BACK TO MAIN .- . . Tho acute toxicity of N2803-4 to the fathoad d n m , Pirrpho1e.a promelas, is described in thim final report. Tho tout was conducted for 38 Company for 96 hour8 fro@ September 25 to 29, 1995, at T.R. nilbury Laboratorios, Inc. in Xarblehoad, Xaasachusetts. I:t vae conducted by Peter L. KWAlski, Jeanne mgazu, JaCqUe1ine Nevium, )<evenStevens, Peter NOViUn, Robert Boeri, and Timothy Ward according to t h ~ aprotocol developed for T.R. wilbury study Number 894-Tfl. N2803-4 VILB supplied by tho rponsor. The teat was performed under static conditions with five concentrations of test substance and a dilution water control at A temperature of 21.6 to 22.7.C. The dilution water wail filtered deionized water collected at Harblehead, Hassachueotts and adjusted to A hardnoss of 44 mg/L as caco,. Aeration was not required to maintain dismolved oxygen concentration. above an acceptable level. Nominal concentration8 of N2803-4 were 0 (control), 160, 250. 400, 630, And 1,000 mg/L. Fathead minnws usod in the teat were procured from A commercial supplier and acclimAted to test condition. at T.R. Wilbur: Laboratories. After 96 hour. of exposurfrthe control fish had an average wet weight (blotted dry) of 0.19 g and an average total. length of 28.6 nun. All fish were in good condition at the beginning of the study. Exposure of fathead minnows to the test substance resulted in a 96 hour median lethal concentration (LC50) of 7 4 0 mg/L N2803-4, with a 959 confidence interval of 660 to 830 mg/L. The 96 hour no observed effect concentration (NOEC) is 400 mg/L. T.R. wflbury study Number 8 9 4 4 f l Page 6 of 22 BACK TO MAIN .I V I . OCNBRAL IIROIWITION Pest Substance Shipped Iromr Reported Purity: Study Initiation Date: Experimental Start Date: Experimental Completion Date: Study completion Date: Archive Location for the Biological mw Data and a Copy of the Final Report I 12803-4 3M Company environmental Laboratory 935 Buih Avenue Building 2E-3L-09 St. Paul, Minnesota 55144-1000 314 3H Center st. Paul, Hinnesota 55144-1000 100% August 116, 1995 (protocol signed) September 25, 1995 September 29, 1995 November 21, 1995 T.R. wilbury Laboratoxiee, Inc. garblehead, nassachusett s T.R. nilbury study Number 894-TH BACK TO MAIN - VII , ImrnowcTIon This study was rponsorod by 3M Company, 5t Paul, KLnnosota. Tho objoctivo of the study WAS to dotormino tho 24, 48, 72, and 96 hour Dsdian lethal concontrations (LCSOs) of tho test substanscr to tho fathoad minnow, P h p h e l o 8 prumoles, A froshwator fish, under static conditions. The tost protocol WAS based on U . 8 . EPA mekhods (U.S. EPA, 1993). Tho roport contains sections that describe the Iruthods and materials employod in tho study, and results of the investigation. The rtsport also contains an appondix that prononts water quality data collectod during the test. VIII. UETEODS AIID WTERINAI mST SUBSTMlCC: Tho sample of N2803-4 (T.R. Wilbury Laboratories Sample Number 544) waa delivered to T.R. wilbury Labor.rtories on September 22, 1995 in a 250 ml glass bottle within a cardboard box at ambient temperature. The attached label included the following information% '"2803-4, Corrosive, 8.5. flartin, 53-4N-02778-5774. 9/13/95-. N2803-4 (a white powder) WAS supplied by 3fl Company, St Paul, Minnesota. Prior to use the test substance was stored in the dark at room temperature. Test substance stock solutions were formulated in dilution Water without the use of a solvent. The test sutistance was assumed to havo a purity of 1000 active ingredient and to be stable under storago sod . testing conditions. spocsor unused test substance will. be returned to the DILUTIOls W m R : Water used for acclimation of test organisms and for all toxicity testing was deionized water collected at T.R. Wilbury Laboratories in W b l e b e a d , mssachuaetts. Water was adjusted to a .hardnessof 40-48 mg/L as CaCO, and otored in 500-gallon polyethylene tank13where it was aerated and continuously passed through a particle filter, u:Ltraviolet sterilizer, and activated carbon. Results of chemical analysis of a representative sample of water are presented in Tabla 1. 2ssz O U D l l i I S x * Juvenile fathead minnows, Pimephales promelra, employed organisma wore from a single sourcs and were procured from Aquatic Bioaystoms, rort Collins, Colorado on September 6, 1995. The the fish was verified using an appropriate taxonomic key. Page 8 of 22 BACK TO MAIN Tal. 1. chomical charactorization of a rmpresmntativm a m p l e of dilution watmr umed for ,the toxiaity tmat with fathead dnnovr, Pf~nopbalo#promola#, and 112803-4. ParAmmter1 unit of Xeamurement mtmction Limit )(..urd Value .. notal. Aluminum Armenia Boron C&dmiUUl chromium cobalt copper Iron Lmad nercury Nickel silver zinc Nitrate Chloride Fluoride Total Orgcnfc Carbon Total Phosphorus Organochlorine Pesticides Toxaphene Organophosphorus Pesticides Dhthoate TEPP Honocrotophoa PCB8,; ,' mg/L mg/L mg/L mg/L Qg/L mg/L 0.1 0.01 0.5 0.0002 0.01 0.03 0.005 0.03 0.005 0.0003 0.03 0.02 0.02 0.05 1 0.1 1 0.03 0.5 2 0.5 2.0 2.0 2.0 0.5 NDZ ND ND m ND ND ND 0.03 ND UD PD ND ND ND ND ND ND ND ND ND ND ND ND ND Notes: 1. mtals, nitrate, chloride, fluoride, total organic carbon, total phosphorur, organochlorine pesticides, organophosphorus pesticides, and PCBs were meairuredin deionized water (used to formulate dilution water) that wan collected during August, 2. -1995 and analyzed by Pace, Inc. quality testing. ND not detectmd at or above the as part of routine detection limit. water BACK TO MAIN i Prior to temting tho fimh y.ra maintained under flow through conditionm in a 270 liter fiberglass trink or a 40 liter glarr aquarium. 1 During acclimation fish were not treated for dimoamel, thoy were free of apparent micknorm, injuriem, and abnonmlitiom At tho boginning of tho tort, and mortality during the final 48 Irourm of acclimation was less than 3a. During the 14 day period before the start of the test the acclimation temperature rango warn 21.0 to 21.9.C 8nd the dismolved oxygen concentration warn alwaym at least 7.7 mg/L. During ac:climation fish were fed a dry commercial food (Tetra win. staple Food, Lot # TH05) daily except during the 48 hours immed!ately procoding the temt. Pimh woro not fod during the test. At the conclurion of the test the control fish had an average total length of 28.6 m and an average wijt weight of 0.19 q (loading rate during the definitive toxicity temt wa# 0.13 g/L). TOXICITX TCSTIUG: The definitive toxicity test was pctrformed from September 25 to 29, 1995, according to T.R. wilbury Protocol 894-TH (Acute Toxicity of N2803-4 To The Fathead Minnow, Pfmephalea promelas). The protocol, which waq migned by the study director on August 16, 1995, is based on procedures of the U.S. Environmental Protection Agency (1993). A slumnary of the test Byrtem i m presented in Table 2. The test was conducted at a target temperature of 22 f 2.C with five concentrations of test substance and a cLtlution water' control. Nominal concentrations of the test substance we:ce: 0 mg/L (control), 160, 250, 400, 630, and 1,009 mg/L. Appropriate amounts of test substance were added directly to dilution water in test vessels to formulate test media without the use of a solvent. Twenty fathead minnows were indiscriminately and oqually distributed among two replicates of each treatment. The test was performed in 20 liter glass aquaria that contained 15 liters of test solution (water depth was approximately 1 E cm). Test vessels were randomly arranged in a water bath during the 96 hour test (a rcindom numbers table was used to select the location of each vessel) and they were loosely covered. A 16 hour light and 8 hour dark photoperiod with a 15 minute transition period Was automatically maintained with cool-white fluoreacent lights that provided a light intensity of 30 footcandles. T.R. wilbury study Number 894-TH page 10 of 22 BACK TO MAIN , T ~ b h2 . Toat 8yatom a c m u r y for t h o t o x i c i t y t e a t w l t h N2803-4 and f a t h e a d minnow., Pfmephalos promelas. -1 8p.ci.a I Pimephales promo1&a Ago/Size of T e s t Organisma: Juvoniles/-O.19 girama, Vot woight Tort Duration: 96 hours Teat Vesselr: 20 1.Lter g l a s s a q u a r i a Depth of Test n e d i a i 18 cia Number Of R e p l i c a t o T e s t V e S s O l S : 2 Organioms p e r Replicate1 10 Temperature Range: 2 1.6-22- 7.C Hinhum Dissolved Oxygen: c.7 nig/L Photoperiod: 16 hours l i g h t and 8 hours d a r k w i t h a 15 minute t r a n s i t i o n p e r i o d . Light Intensity: . . Water Quality Heasurements: 30 fcotcandles D i s s o l v e d oxygen, pH, t e m p e r a t u r e , and conductivity d a i l y i n each test vessel. Temperaturemeasured at least e v e r y hour i n 1 t e n t v e s s e l . Hardneea, alkalinity, acidity, and p a r t i c u l a t e matter and TOC in t h e d i l u t i o n water. D i l u t i o n water ana1.ye.i~ is summarized i n Table 1. End P o i n t s : 25, 58, 72, and 96 hour LC50; 96 h o u r NOEC. Acceptability Criteria: slO% c o n t r o l m o r t a l i t y and a c c e p t a b l e temperature range. T:R. U i l b u r y Study Nunrbor B94-Til -- Pago I1 of 22 BACK TO MAIN Tho numbor of murviving organimmr and tho O E C U ~ o~f ~mu~blCot~hal offoctm (immobilization, loam of equilibrium, loam of roflox, oxoitability, dimcoloration, or change in behavior) war. detorrlnod ViDUa1ly and rocordod initially and aftor 24, 48, 72, and 96 hourm (mortality was monitored twico each day). Doad tost organimnu woro r o m v o d whon firmt observed. Dimsolved oxygon ()!SI )lode1 57 motor; iomtrurunt numborm l), pH (Beckm6n modo1 pHT 4 meteicy instrument number 1 2 0 , conductivity (Colo Parmer mater, inatrumonit number 9 0 , and tomperaturo (Beckman model pHT 4 nmteeri instrument number 124) were manured and recorded daily in each 1:est chamber that contained live A n h l m . The temperature in one test veosel was recorded during the test. STATISTICAL )tslgODSr Result8 of the toxicity test were interpreted by standard . Statietical techniques. Computer methods (stephan, 1983) were used to calculate tho median lethal concentrations (LCSOs) A.11 calculations were performed by w i n g nominal concentrationr of test eubstance and the number of livo fathead minnowa. The no obmerved effect concentration is the highemt concontration of test substance that did not cause any toxicantrelated mortality or sublethal effects. T.R. Wilbury study Number 894-TH Page 12 of 22 . ... . - ~ . BACK TO MAIN 11, IW8vLT8 Inrolublo matorial ua8 not notod during tho test. Biological and vator quality data gonoratod by tho acuto toxicity tomt aro proronted in Tablo 3 and Appondix A, r08poctivolly. The moamurod concontrationm of dia8olved oxygen ranged from 6.7 to 8.5 mg/L, the temperaturo ranged from 21.6 to 22.7.12, conductivity ranged from 140 to 380 pmhom/cm, and pa rangod from 7.3 to 7.6. on. hundrod percent murvival occurred in the control exposure and theso fathead minnow did no(: exhibit any 8ubletbal effect.. The 24, 48, 72, and 96 hour Lc50a for fathead minnows exposed t o N2803-4 are preaented i n Tablo 4. Tho 96 hour Lc50 for fsthoad minnov8 exposed to N2803-4 ir 740 mg/L, with a 95% confidence interval of 660 to 830 mg/L. The slope of tho 96 hour dose responfie curve is 10.1. The 96 hour no observed effect concentration (NOEC) i n 400 ng/L. T.R. wilbury Study Number 894-TH Page 13 of 22 BACK TO MAIN Tab10 1. survival and rublothal affect data frtw tho toxicity tost with fathead minnowr, Pimepbrlea prontelaa, and Y2003-4. Nominal Concentration Of N2003-4 (WL) Rep. Numl3o.r alive 0 24 4 0 12 96 hr hr hr hr hr WrPber affected 0 24 4 0 72 96 hr hr hr hr hr 0 (control) 1 10 10 10 10 10 2 10 10 10 10 10 00000 00000 160 1 10 10 10 10 10 00000 2 10 10 10 10 10 00000 250 1 10 10 10 10 10 00000 2 10 10 10 10 10 00000 400 1 10 10 10 10 10 00000 2 10 10 10 10 10 00000 630 1 io io to 9 a 00000 2 1010877 00000 1,000 11010222 2 107000 0 0 2 0 --0 --0 -0- ~ -~~ Note: Affected fish were dark colored and swimming erratically. T.R. Wilbury Study Number 094-TU Page 14 of 22 BACK TO MAIN ~ ___.--- - 1 7 - -1 - - - 0 160 250 400 630 1000 Concentration 01 N2803-4 (rng/L) Figure 1. Survival of fathead minnows, P-bephales promelas, exposed to N2803-4 for 96 hours. T.R. Wllbury Study Number 894-TH Page 15 of 22 BACK TO MAIN T ~ b l e4 . H e d i m l e t h a l c o n c e n t r a t i o n s (LC50r) from the toxicity t o r t w i t h fAth0.d 8tiflflOW., P h O p h A l . # prOlDdi.8, and N2803-I. Lxprur. period ~ LC50 ~~~ 24 houra r1,000 mg/L 95 porcent confidence limit -- calculation Mtbd -- 48 houra 790 mg/L 630 t o 1 , 0 0 0 mg/L BinoldA 1 72 hours 760 ng/L 630 to 1 , 0 0 0 mg/L Binomial 96 hours 740 mg/L 660 to 830 mg/L Probit T.R. Wilbury study Numbor 894-TH Page 1 6 of 22 BACK TO MAIN .. .... - - . x. R I l t R P ( C L I ASTX. 1986. S t a n d a r d P r a c t i c e for Conducting Acute T o x i c i t y Temtm w i t h rimhem, H a c r o i n v e r t e b r b t o s , and Amphibian.. t-729-80. 3 Annual Book of S t a n d a r d s . Stephan, C.E. 1983. Computer Program for t h e Ca1cu:Lation of LC50 Values. U.S. EPA. Duluth. m. Permonal Comnunication. U.S. EPA. 1993. Toxic Bubmtancem C o n t r o l A c t T e s t G u i d e l i n e s ; F i n a l Rules. S e c t i o n 797.1400. . U-S. LPA. 1993. 40 CFR P a r t 792. Toitic Substanceai C o n t r o l A c t (TSCA); W d Laboratory P r a c t i c e Standairdm Code of F e d e r a l R e g u l a t i o n s , T i t l e 4 0 , JULY 1, 1993. T.R. w i l b u r y s t u d y Number 8 9 4 - ~ n Page 17 of 22 .. BACK TO MAIN Bioflogimt Coauthor it21 9 > T.R. Wilbury Study Number 094-TU BACK TO MAIN ......_ .-.- .. ... .-.. The p r o t o c o l was amended to s p e c i f y t h e nominal c o n c e n t r a t i o n s of test substance for t h e d e f i n i t i v e t e i t rind t h e source of t h e t e s t organisms. PROTOCOL DSVIATIOWS: Temperature was recorded c o n t i n u o u s l y e x c e p t for t h e l a s t t w o hours of t h e s t u d y . T h i s d e v i a t i o n had no e f f e c t on t h e outcome of t h o study and no o t h e r d e v i a t i o n s were made from the p r o t o c o l . T.U. Nilbury Study Number 894-TH Page 19 of 22 . .. .. BACK TO MAIN Appondix A. WATER QUALlTI DATA IR- MXICITX "KST i T.R. wilbury Study Number 894-TH Page 20 of 22 I i 3 BACK TO MAIN ' Tablo A.1. Dimrolvod oxygon concentration, conductivity, pH, and tmmporaturm abASUrbd during tho toxicity tbrt with fathoad mlnnwm, P i m e p h ~ l o wpromelan, and W2803-4. Nominal Concentration of N2803-4 (mg/L) Rep. Dirrolvod Oxygon (nq/L) 0 24 48 72 96 hr br hr hr hr -Conductivity (jmhom/cm) 0 24 48 72 96 hr hr hr hr hr 0 (control) 1 2 160 1 2 250 1 2 400 1 2 630 1 2 000 I 2 8.5 7.2 i . 5 7.2 7.5 8.5 7.4 7.5 7.3 7.6 8.5 7.7 7.5 1.1 7.4 8.5 7.7 7.5 7.2 7.5 8.5 7.8 7.5 7.1 7.6 8.5 7.8 7.5 1.0 7.6 8.5 7.7 7.5 7.1 7.4 8.5 7.6 7.5 7.1 7.2 8.5 7.6 7.4 1.0 7.1 8.5 7.4 7.4 6.8 7.4 8.5 8.5 7.7 7.6 7.0 1.1 6-.7- 7-. 2- 140 180 160 160 150 140 180 160 160 150 160 210 170 160 160 160 210 170 170 160 170 230 180 180 180 170 230 180 180 180 1'90 260 210 200 2 1 0 1'90 260 210 2 0 0 210 2150 310 270 2 6 0 260 2!50 310 260 260 2 6 0 2130 2130 380 380 340 350 3-50- --340 T.R. Wilbury study Number 894-TH -- .. . . . ..- ~ .. .... .. . ..~. ~ ~ BACK TO MAIN Tabla A.1. Continued. Nominal PH Concantration O f N2803-4 0 24 48 72 96 ~ e p . hr hr hr hr hr .. Temporatwe (*c) 0 24 48 1 2 96 hr hr hr hr hr 0 (control) 1 2 160 1 2 250 1 2 400 1 2 630 1 2 1,000 1 2 7.3 7.4 1.5 7.5 7.6 1.3 1 . c 7.5 7.5 7.6 7.4 1 . 4 7.5 7.5 7.6 7.4 7.5 1.5 7.5 7.6 7.5 1.5 7.5 '7.5 7.6 7.5 7.4 7.5 '7.5 7.6 7.5 7.5 7.5 '1.5 7 . 6 7.5 7.5 7 . 5 '1.5 7.5 1.5 1.4 7.5 7.5 7.5 1.5 1.4 7.5 7.5 7.5 7.5 7.5 7.4 1.4 7.5 7.5 --7.5 7-.5- 22.2 22.2 22.1 22.5 22.1 22.2 22.2 22.0 22.6 22.1 2.2.0 22.1 22.1 22.6 2 2 . 1 2.2.1 22.1 22.1 22.1 22.0 21.8 22.0 22.0 22.5 22.0 21.9 22.0 22.2 22.4 22.0 2l.6 2 2 . 1 22.1 2 2 . 5 22.0 2:i.a 2 2 . 1 22.0 22.5 22.0 22.0 22.1 22.0 22.6 22.0 2l.9 22.1 22.0 22.5 22.0 21..8 22.0 21..8 22.0 22.0 22.1 --22.5 --21.9 T.R. Wilbury Study Number 894-TH Page 22 of 22 Lnv-iv1t.L .- ~ ~ ~ u t i ~ I ARCHIVED FINAL REPORT LAB REQUEST NO. N2803 REQUESTOR NAME: SAB/GOEBEL/PICKETT DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 08/01/1995 DESC: FC-143 AND RELATED PRODUCTS ECOTOX WORK CONTRACT LAB(S) : TRWIL EXP COMP DATE: 10/04/1995 DATE C0MPLETE:D: 03/27/1996 PROIJECT .LEAD:: PHONE NO: 3M FAX NO: 651-778-6176 BACK TO MAIN Samples received from S.J. M a r t i n . Samples 1 - 5 sent t o TRWIL on 8/11/95. 8/22/95. An a d d i t i o n a l 4L of sample 5 sent on Sample 1 = T e t r a b u t y l ammonium hydroxide, 40% i n water. Sample 2 = L-13492 (45% s o l i d s , 27% water, 27% IPA) Sample 3 = FC-26, 100% s o l i d s . Dissolve i n 50:50 wator:IPA sol.ution. Sample 4 = FC-143, 100% s o l i d s . Sample 5 = L-13856, 15% s o l i d s . Sample 6 = L-13856, d i l u t e d by a f a c t o r o f 15, t e s t e d as an e f f l u e n t . Sample #1 (Tetrabutylammonium hydroxide) - a new bottlte was ordered from A l d r i c h because TR Wilbury needed more sample. This was l o t 05922BN, r e d l a b e l , c a t . # 17,878,O. sab 9/11/95 NOTE: A l l data f o r sample 3 (FC-26) i s r e p o r t e d i n terms of FC-26, and n o t as a 50/50 mixture w i t h isopropanol. sab 11/21/95 NOTE: For sample #5, o n l y a r a n g e - f i n d i n g t e s t was run f o r t o x i - c i t y t o algae. The requestor canceled t h e request f o r t e s t i n g so no d e f i n i t i v e t e s t was run. The r e s u l t s f o r t h e range f i n d e r are as f o l l o w s : Test Conc. mg/1 0.1 1 .o 10 100 1000 EC50 (95% C.I.) sab 2/16/96 Percent Difference i n Avg. S p e c i f i c Growth Rate! 96-hrs Expsure 2 18 4 32 160 100 (100 - 1000) mg/L. For N2803-6, t h e t e s t i n g was canceled before a d e f i n i t i v e study w i t h f i s h could be completed. Results r e f l e c t t e s t i n g w i t h a s i n g l e conc,entration o f 13% v/v, which showed no m o r t a l i t y a f t e r 96-hours. sab 3/14/96 1 08/11/95 TB NH40H L o t 10317KQ TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST 27 MG/L (23 - 32) 04/01 /2001 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT BACK TO MAIN 2 1 0 8 / 1 1 / 9 5 TB NH40H Lot 10317KQ - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0 720 MG/L 3.7 MG/L - (600 1000) (1.8 - 7.7) 2 L-13492 Lot 2 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL IC50-ALGAE *ALGAE ASSAY I C 5 0 3 FC - 26 L o t 135 - TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0 62 MG/L 960 MG/L 8.4 MG/L 360 MG/L 140 MG/L 9 0 MG/L (36-100) (830->1000) (5.9 - 14) (300 - 500) (110 - 180) (63 - 130) 4 FC-143 L o t 427 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0 5 08/16/95 L-13856 As -is 720 MG/L 740 MG/L > l o 0 0 MWL (600 - 1000) (660 - 830) (-1 - TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0 190 MG/L. (130-600) > l o 0 0 MG/L (-1 SEE COMMENT ABOVE 6 L - 13856 1 :15 d i l u t e d TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL ICSO-ALGAE *ALGAE ASSAY I C 5 0 1.2 % VI'V (1.0 >13 % VI'V (-1 TESTING CANCELED - 1.7) * = CONTRACT LAB DATA