Document yr9542ODwK2aypb1JQ9xg3god

i O'l This effect was not calLdivision. MC, whicn asfc with 9-aminoacridine s in the SCE induction nts. However, the differould be taken into conan leucocyte test system ndly supplying the acri1 analysis of the results, for her technical assis- onal Cancer Programme t sensitivities ot sub-populations irn the first Gl-prophase of the on with PH A, Hum. Genet., 35 iberration yields in the human E frequency and ccU kinetics in cycle time in culture measured 3-385. imor compounds, XII. Biologic ninoacrldlnes, Arch, Immunol. 1 K. Unoazko, A search for anti n' l-nitro-9-amlnoacridines and inn of sister chromatids, Nature tie and chromosome aberration tl. Cancer Inst., 9 (1977) 289-- rersitu ity of phytohaemaRjdu7 il973) 367-376. roma.ul exchanges of restricted 4. 1-201. tumour inhibiting compounds, nikl Chem., 51 (1977) 1725- Mutation Research, 67 ( 1979) 97-- 100 Elsi-vipr'.N'orth-Holland Biomfdic.il Pre-s Short Communication COMPARATIVE EVALUATION OF THE FREQUENCY OF CHROMOSOMAL ABERRATIONS AND THE SCE NUMBERS IN PERIPHERAL LYMPHOCYTES OF WORKERS OCCUPATIONALLY EXPOSED TO VINYL CHLORIDE MONOMER M. KUCEROVA, 7. POLIVKOVA and J BATORA * Pediatric Department of Postgraduate Medical Institute, institute of Hygiene and Epidemiology, Prague, Srobdrova 48 (Czechoslovakia) (Received 31 March 1978) (Revision received 15 November 1978) (Accepted 23 November 1978) Therc arc now available plenty of authorative data on the carcinogenic j 1--.'1, Id] and mutagenic [1,2.10] activity of vinyl chloride monomer. Increased frequency of chromosomal aberrations detectable in peripheral lymphocytes of chronically exposed workers has been reported by numerous authors [4~6, 11,12]. We have repeatedly analysed, using routine cytogenetic techniques 18], .3 blood samples from each of 9 workers occupationally exposed for 10--27 years to relatively high mean annual doses of vinyl chloride monomer (about 20--150 ppm of air). In the third blood sample series we also used the SCE technique [1 ij and compared the frequency of detected chromosomal aberrations with the number of SCEs m each sample. The blood samples from 8 healthy persons were used as controls and were collected with the 3rd blood sampling from the vinyl chloride (VC) workers. The control persons were matched for age and sox and they were not exposed to known mutagens during 3 months before the time of blood collection. The results are summarized in three tables. Tables 1 and 2 show the instabil ity and non-homogeneity in the frequency of chromosomal aberrations over a period of two years. Mostly chromatid and chromosome breaks were detected; chromatid and chromosome exchanges occurred only sporadically. Table 2 compares the frequency of chromosomal aberrations and the num ber of SCE per cell m 7 blood samples. There is an apparent increase in both parameters in comparison with control levels. The mathematical evaluation of our results was calculated only for the 3rd collection. Conventional aberrations m VC workers were non-homogeneous. * District Hvjuenic -SUtUHi. Pnewd/.i, i. This effect was not 11 division. MC, wInch -siv 1 minoai mime Mutill VH h't''{(}rt. > >'] 7 / ] t, o ; 0 * j (i; SL 101622 ** 4 t^ 99 (ILK 2 I'll'SD'HI. \\ U.YSKS OK 9 WOUKERS OCCL P ATIO N M. L Y FXPOsED TO VINYL CHLO- \ posurt* ,rs Pl'r I si < ollcrtnm ib riant t > Us * `inti colli-nuMi aberrant dt-lK 3rd coll ctu-n iIxTram *'* lis sCl- cUl h 27 IH !7 tm J1 ;i ii lo 12 2 (K 1 M) 20 HO 1 "i "0 '2 l > I ;>o 20 00 JO i r.o 2" "0 21 > Hi hi) lt l 2 I 0 a i i 4 0 '> 7 I 1 h 4 2 1 h * an) t i re Nt'nri'il pc t `v.niiplt' iT1 pi-t t*mi pl<' ' 1 v J M 1 fr.jsoil 1|r; C'ompdl-Json a jib i nm/ul s jlufs. 1l c 2 'i 7r 1 1 l' 7, l J r>.2 * i.j 1 8 * 04 17 :>o i' 9 fp; 1 ') 0 2 c lo.2ti c 10 41 c \ 0 hO 1 2 22 l' 1,1 R0 M.13 941 i 0 40 iuct'd chromosomal chany(*!i depend both on dose and length of exposure to v vinyl chloride monomer. Our result., seem to lead to a conclusion that the two cytogenetic methods -- utine and St'F, are equally suitable for testing of high-dose vinyl chloride ntaceiueitv m vivo. The increase of SCKs in our results is more homogeneous. ie v umuviUcs of the two methods to detect mutagenic activity seem to be ini lie 'nine un.i :i im U Ills I o!M , .\\|L> SMOKING \NU DRINKING HABITS OF WORKERS TESTED r.idhil.iili ! I U Smoking Drinking ; ++ t *. Drugs Menrohamai Hdpjvt rin Chloramphenicol b>m<jph>lhn Solulan Sv niophi 11m B'-ildspon Supt'rpv rm Thiospasmin Kt'id/oti  th > 'I AH L I I CHROMOSOMAL \ \ \ i A MS OI' K fO\ 1 ROLS A0- i mi nils J (*' ) i,C f (.I'll b M,k. J, K V.H. -I.S KB M ku Z.P kA Mean 3 0 3 3 1 1 2 1 1,8*04 10 88 K 22 10 30 1 0 1 (i 10 49 8 20 7,9 4 9 02 9,11 ' 0.40 .smoking ++ +* * Id MlMlU1 '* LOO fell's ',ur*` scared pu sample ^ 50 cells v, ere scon d per wu plo The controls were not exposed to Vrass and drugs .n the tune ol blood collection References L Bartseii. II, C Mabis otlli* and It Monios<j_;ii`, Human tat and nnm-a hs i r-mcai iied mutag* naps oi v in v| chloride m "> t\ /`i mi if tiitn -dr.uns, In t -l ( am i r. I 5 ( I 0 7 5) 129 407 2 B*rt$ch, H uni It Mi'iiics.ni", Mutagenic and l uri mugenu elfccls of \mvl chlonde, Mutation lies 32 (1975) 93-1 I 4 3 Creech. 1,1,,. and M A .iohnson, \nniotarcoma ol 'he liver in the manufacture of polyvmsl chloride. J, Ouup, Med .19(19741150 151 4 Ducatman, V' K Hirschhorn and I .1, Sehkoff, \ invl chloride exposure and human chromosome aber* rations. Mutation Re* , 3 1 < 1 07,">> 193-l fid, 5 FuncS'Craviotn, V . B Lambert, J Lindsten, L Hinmuerg, A,'I, Nataraian and S OstennanMiolkar, Chromosome ahei rations in w orke'rs e \ post d ' n . in\ ! eldond . Lain d. 1 11 97 5 j 159 G Hanstecn, 1 L , L Hillestud and ! ('hus-l vrusui, Chromosome studies in workers exposed to Mini chlonde, Mutation* Res., 38(19791 11 2 7 Hdn^tocn. I1. , Chiomovome breakage f lepurncs and sister chromaudi cm hang* s in I'V (J ss or kern t w o stars aiu-r exposure, \ fol!oss-ui> studs \bstrui t ol Gth lnternation.il ( liromusmin Conf' n-ncr Helsinki, \ugus*. 1077 8 Hunger ford. D \ , Li ukoes 9 s cul tu n d f ro*n sm ah imu -da o I -.hole blond ,nnl pre 1 < i > ttion of no ta phase chromosomes bs treatment a n 9 h * ine mu Is Cl, stain Ii lino|, t u (l `Min) 333 338 9 luifi H'vj, \I . C' tnge netlf anal v Si S 1 * I n I'l.m i liri'iiinsiiiili's .illil Its idlin' f'n t lie t Slim at o m " f _ene V e nsk. Mu tainm Res . 41 U 07b i 123 130 10 Lopneno, "s' , R Bara'u , S Baron. - ill ( B,. - r n Bron/ctll, \ fhm im'llin i, (i C*'i >`lgnan l < <o O Gersasi, C Leponm K \nri, \ M H-aM ( -trelll and </, J arelii, / v.du.dimi of tin m ! effects induced hi sms 1 eldond< (\C,M) under mammalian metabolic activation m siti .uni in s i\ o if udies, M nation H > s , 4 0 (1 urn, i h'i of; 1 1 Purchase. U' H , C Richardson ,nnl I> \mi.*rsou, ( luomoiom.il ,m i dominant lethal effects of un chlonde. Lancet, ll (1 97,j) HO 111 1 2 Purchase, Li- H , C K ichai dxi m and D \nderson, Chi mios. >mal e 1 f< c is in p* npher.il Is mphucs te*Proc, Ros. doc Med , o9 (l 97 p) *290 *jii i 13 Viola. P.L.. A Bigotti and A, Caputo, Oncogenic response o| i.u skin, lungs and hoims to smsi chlo nde , Cancer Res , 2 l (1971) 51G -522, 14 Wolff. S , and P Peirs , Diffcn maul (m-msa Maming oj msO t .'Inomatids and the studs of sistei chro malid exchanges w ithou t au toranmgraphs Clmmiovuna, I8ii;i74)341 353 SL *01625