Document ypw3Nz6yobj9yyadpoMqbNj6E

\ J - Copl;es to AR226-2857 Material Tested ------3 HaskelI HASKELL [ E. I. du Pont de Nemours rind Company Laboratory for Toxicology and IndustrialIJ4BIdiclne LABORATORY REPORT HO. 430-77 MR NO Haske)1 No. 11,154 Sample Ready For Testing 3-7-77 Other Codes ^ Ma Departme PRIMARY SKIN IRRITATION AND SENSITIZATION TESTS ON GUINEA PIGS Proced u re; The test for primary irrlta^on was conducted by applying, and lightly rubbing In, I of a 50^ and a 5% solution (vol/vol) of the test material In distilled water on the shaved Intact albino guinea pIgs.S To test for the sensl+lza+lon potential, a series of four sacral Intraderma one each week over a three-week period, which consisted of O.I ml of a \% solution (vol/vol) of t Following a two-week rest period, the test animals were challenged for sensltlzatlon by applying, I drop (=0.05 ml) each of a 50? and a 5% solution (vol/vol) of test material In distilled water o shoulder skin. A group of 9 previously unexposed guinea p'Igs received similar applications at th provide a direct comparison of the challenge reactions on skin of similar age. After an addition fhe animals were rachal longed following the same procedure as for the challenge as a further chec Results; Concentration In distiI led water Primary Irrl+sit Ion Test-24 Hours -48 Hours Challenge Test-24 Hours -dQ Hours Sens1+lzatIon Response Ratio*" r<w.hallenge Ti9St-24 Hours -48 Hours ^orisl t litatlcin Response Ratio** ,10 Animals - In. Avg. Wt. - 5^9 g Avg. Wt. 738 g Rechallenge Avg. W-t. ' 7W g) 50% 5{( 10 neg. 10 neg. 1++, 4+, 5 4+, 6 neg. neg. 10 neg. 10 neg. 1+, 9 neg. 10 nag. I/I 1++, 6+, 3 neg. 5-f, 5 neg. (0 nog. 10 neg. I/ <9 New Anima Rechalle W% 9 neg. 9 neg. 1+, 8 neg. 1+, 8 neg. -2- Summary; ^HBHBj'^" 11.134) produce, no irrf+a+lon when testod as a 50!( and a 5% solution In dis shaved Inract skin of male albino guinea pigs. The +est for sensi+lza+lon Indicated that 1/10 anim mild to very mild reactions observed !n several others. A similar difference 'n response of test ov seen when rechallenged. This material Is a weak sensl+lzer; therefore, skin cent-act should be avoid washing if contact should occur. Other name Composition; * Reaction Code: +. ++ +++ = mild, moderate, strong erythema; ++++ = erythema plus edema; +++++ '" ?-'-,',3itization is defined as a significant score increase at challenge over the response expected applied Initially or on the concurrent controls. 5 Preliminary range-finding date indicated mild Irritation when tested as received (lOOjt). fCG;ms Report No. 430-77 Date Issued; June 10, (977 Report by: C. ------T Nancy C.^eo Technici Approved by; , ^ ^ ^ ? . 7 /^ Datjmf\n f4 til