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MICROBICS MICROTOX TOXICITY TEST
IESTSuBsTANCE
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Identity: A mixture containing perfluorooctanesulfonate; may also be
referred to as PFOS, FC-95, or as a `component of FC-206CF or FC-206CF-X. (1-Octanesulfonic acid) (CAS #2795-39-3).
Remarks: The 3M production lot number was 101. The test sample is FC206CF. Current information indicates it is a mixture of 0.5% PFOS, 10% diethylene glycol monobutyl ether, 78.08% water, 1.375% acrylic foamer, 1.5% sodium octyl sulfate, 1% sulfonated alkyl ether salts, 6.0% urea, 0.5% triethanolamine, and 0.05%tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not.accurately reflect the toxicity of the fluorochemical component of the test sample.
METHOD:
000
Method: Microbics Microtox "BASIC" Procedure
Test type: Acute static
GLP: No
Year Completed: 1991
Species: Photobacterium phosphoreum
Analytical monitoring: pH and osmotic condition.
Exposure period: 30-minutes
Statistical methods: EC50 values calculated by statistical linear regression
program provided for Microtox Test organism source: Microbics' Reagent.
Test conditions:
Diluent: Millipore Milli-QTM water
Test temperature: Not noted.
Stock and test solution preparation: A primary 2000 mg/L stock solution was prepared by dissolving 200 mg of test substance in 100
-
mL of Millipore Milli-Q" water. The pH of the stock solution was 6.5,
and an osmotic adjustment was made using 200 mg NaCl dissolved in
10 mL stock solution. Appearance of test solutions was noted as
"clear, colorless". All test solutions were made by proportional
dilutions.
Exposure vessels: 4 mL cuvettes.
Numberofreplicates: two
Number of concentrations: Four plus a blank control Element basis: Percent light loss
R0 ESOW0 TS 000000000
e033
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L.
Element value:
155--mmiinnuuttee
ECso ECso
= =
8>91000(073m0g--/L1000)
mg/L
30-minute ECsp = 680 (610 ~ 750) mg/L
Element values based on nominal concentrations.
Remarks: Substance
Testing was conducted on Remarks field. The values
the mixture as reported apply
described in the Test to that mixture and not
the fluorochemical proportion alone.
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