Document yp450M7835Xdqz6BrMy64d2eE
AR226-3124
HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE
H-24119: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna
PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed
test concentrations and data measurements at predetermined intervals.
GENERAL INFORMATION
WORK REQUEST NUMBER: ^|
HASKELL NUMBER: SERVICE CODE:
MATERIAL TESTED: SYNONYMS/CODES:
| SPONSOR:-]
NOTEBOOK ID: ' SUBMITTER'S NOTEBOOK NUMBER;
COMPOSITION: 'BJ_--_--__--__y/
CONTAMINANTS: -Residual SClVent (THF or MIBK).
^-neutralizatifl CAS REGISTRY
Residual ammonium hydroxide from
PURm
MSDS No: Not supplied by sponsor
TEST CONDITIONS
Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, and 5000 mg/L.
Nominal Concentration
(mg/L)
0 0.5 1.0 50 500 5000
Test Initiation (O hours)
Dissolved
Oxygen
(me/L)
At
Bt
PH
^
^
8.6
8.5
7.1
7.1
8.8
8.8
7.1
7.1
8.8
8.8
7.1
7.1
8.7
8.8
7.1
7.1
8.7
8.7
7.1
7.1
8.7
8.6
7.1
7.1
Test Completion (48 hours)
Dissolived
Oxy gen
(me/L)
At
^
PH
At--------Bt----
8.9
8.8
7.7
7.7
8.8
8.8
7.7
7.7
8.8
8.8
7.7
7.7
8.8
8.8
7.7
7.7
8.8
8.8
7.6
7.6
9.2
9.2
7.1a
7.1-
f A and B represent replicate test chambers within a dose level concentration. a Measurement taken on day 1 (24 hours) due to total immobility.
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Company SanHized. Does not contain T5CA CBI
H-24119: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna, CONTINUED
RESULTS
Based on visual observations, the water control solution and the 0.5 and 1.0 mg/L test solutions were clear and had no color throughout die test. The 58 mg/L test solutions were clear with a suspended substance, the 500 mg/L test solutions were slightly cloudy with a suspended substance, and the 5000 mg/L test solutions were cloudy and white in color with a suspended substance at test start. The suspended substance in the 50, 500 and 5000 mg/L test solutions settled onto the bottom of the test chambers as a white cloudy film throughout the remainder of the test. Immobilities were 0, 0, 0,0,10, and 100% in the 0,0.5,1.0, 50, 500 and 5000 mg/L test solutions, respectively. Forty-eight hours after exposure, lethargy was observed in 20% of the remaining mobile daphnids in me 50 mg/L test solutions, and 33% (3/9) of the remaining mobile daphnids in the 500 mg/L test solutions. The 48-hour ECso, based on nominal concentrations and immobility, was between 500 and 5000 mg/L. All water quality parameters were within acceptable limits.
CONCLUSIONS
H-24119 exhibited low toxicity in a 48-hour, unaerated, static acute test using Daphnia magna.
WORK/AUTHORED BY:
^COLLUJL^7to^kd?&L-
LauurriiccD. Bouchclle Toxicology Associate
APPROVED BY:
PaulW.Grube.B.A. Study Director
l^mne-R^
Day-Month-Year
I g - J ^ l W Day-Month-Year
DISTRIBUTION:
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Company Sanitized. Doss not contain TSCA CBI