Document ympr9aEyqgm0zmEk29w79dbmV

2 2 6 - / 7 6 ,7 Wildlife International. Ltd.________ _____Project Number 454C-101-I -1 - NON-GLP STUDY: DETERMINATION OF THE SOLUBILITY OF PFOS IN W ILDLIFE INTERNATIONAL, LTD. FRESHWATER, FRESHWATER ALGAL MEDIUM, AND SALTWATER ADDENDUM WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454C-101-1 3M LAB REQUEST NO. U2723 This study was reported using a test substance purity o f 98.9%. The test substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requested that the results be revised based on the most recent purity analyses. The measured concentrations previously presented were simply corrected by the ratio o f the two purities or a factor of 0.8787. No attempt was made to recalculate analytical standard concentrations. As such, minor errors due to rounding may be present. Attached is the revised summary page from the final report and the most recent certificate o f analysis. CCN>J> -< f *i r o ~V-{<rr:>] T** ZK /- f rj up no Wildlife International. Ltd. -2 Project Number 454C-10M REVISED STUDY SUMMARY BASED ON A PURITY O F 86.9% SPONSOR: SPONSOR'S REPRESENTATIVE: LOCATION OF STUDY, RAW DATA AND A COPY OF THE FINAL REPORT: 3M Corporation Rochelle Robideau Wildlife International, Ltd. Easton, Maryland :: WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: TEST SUBSTANCE: STUDY: 0 1 Ml# Determination o f the Solubility of PFOS in International, Ltd. Freshwater, Freshwater Algal and Saltwater TEST DATES: -- ---- B-r-------.-T-T- - I mu I TEST SYSTEMS: Experimental Start - January 8,1999 Experimental Termination - March 5,1999 Freshwater, Freshwater Algal Medium, and Saltwater SUMMARY: The mean solubility concentration of PFOS in freshwater @ 20 C after a 24 hour shake period was 326 mg/L 41.9 mg/L (CV = 12.9%; N = 3). The mean solubility concentration o f PFOS in freshwater algal medium @ 20 C after a 24 hour shake period was 378 mg/L 22.9 mg/L (CV = 6.07%; N = 3). The mean solubility concentration o f PFOS in saltwater @ 20 C after a 24 hour shake period was 21.8 mg/L 0.510 mg/L (CV *=2.34%; N = 3). Wildlife International. Ltd. -3 - Project Number 454C-1Q1-1 C ERTIFICA TE O F ANALYSIS INTERIM CERTIFICATE OF ANALYSIS Revision 1(9/7/00) C entre A nalytical Laboratories COA Reference #: 023-018A 3M Product: P F O S,L ot217 R eference#: SD-018 Puritv: fit;*/. COA023-O18A Page I o f3 Wildlife International Ltd., ________________ __________Project Number 454C-I01-1 -4 - INTER1M CERTIFICATE OF ANALYSIS C entre A nalytical Laboratories COA R eference #; 023-018 D ate o f Last Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen < -10#C R e-assessm ent Date: 08/31/01 `Purity * 100% - (sum o f metal impurities, 1.45% +LC/MS impurities, 8.41% +Inorganic Fluoride, 0.59% +NM R impurities, 1.93% +organic add impurities, 0.38% +POAA, 0.33% ) Total impurity from all teste * 13.09% Purity -1 0 0 % - 13.09% - 86.9% P otassium is expected in this salt form and is therefore not considered an impurity. P u rity by DSC is generally not applicable to materials o f low purity. N o endotherm was observed for tins sample. 4Sulfur in the sample appears to be converted to SO* and hence detected using the inorganic anion m ethod conditions. The anion result agrees w ell with the sulfur determination in the elemental analysis, lending confidence to tins interpretation. Based on the results, the SO* is not considered an impurity. 5T F A HFBA NFPA PFPA Trifluoroacetic add Heptafluorobutyric add Nonofiuoropentanoic add Pentafluoropropanoic add `Theoretical value calculations based on the empirical formula, C tf nSO jK * (M W =538) This w ork w as conducted under EPA G ood Laboratory Practice Standards (4 0 C F R 160). / COA023-018A Page 2 of 3 Wildlife International. Ltd. -5 - Project Number 454C-101-1 INTERIM CERTIFICATE OF ANALYSIS C entre A nalytical Laboratories COA R eference #: 023-018A LC/M S Purity Profile: Im purity C4 C5 C6 C7 T otal w t/w t % 1.22 1.33 4 .7 2 1.14 8 .4 1 N ote: The C4 and C6 values were calculated using the C4 and C6 standard calibration curves, respectively. The CS value w as calculated using the average response factors from the C4 and C6 standard curves. Likewise, the C7 value was calculated using the average response factors from the C6 and C8 standard curves. Prepared By: ................................................ .......... . ... ____ ; David S. B ell D ate Scientist, Centre Analytical Laboratories R eview ed B y. ________________________ l _______ John Flaherty ____ D ate Laboratory Manager, Centre Analytical Laboratories COA0Z3-O18A Page 3 o f 3 ildlife International, Ltd. ECOTOXICOLOGY & ANALYTICAL TESTING SERVICES November 27,2000 Ms. Susan A. Beach 3M Corporation Environmental Laboratory 935 Bush Avenue 3M Building 2-3E-09 P.O.Box 33331 St. Paul, Minnesota 55133 Dear Susan: Enclosed please find Addendums for the following studies: 1. Non-G LP Study: Determination of the Solubility of PFOS in Wildlife International, Ltd. Freshwater, Freshwater Algal Medium, and Saltwater Wildlife International, Ltd. Project No.: 454C-101-1 3m Lab Request No. U2723 2. Determination of the Water Solubility of PFOS by the Shake Flask Method Wildlife International, Ltd. Project No.: 454C-107 3m Lab Request No. U2723 If you have any questions, please let me know. RLV/cyw8/3m Enclosures Raymond L. Van Hoven, Ph.D. Scientist 8598 Commerce Drive Easton, Maryland 21601 -Tel.410-822-8600 Fax 4 10-822-0632 * E-mail ecotox@wildlifeinternational.com European Office: Bergkampweg I 7231 CL Wamsveld -The Netherlands Tel. 3 1(0) 575 - 573848 Fax. 3 1(0) 575 - 5 7 4 8 13 E-mail: aleopold@worldonline.nl