Document ymYKKawv2opQez9ppmX75XL3r
AR 226 _ 0739
BIODEGRADATION (OECD 301E)
TEST SUBSTANCE
_
pr
Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m2a0y3Aa.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3).
Rsaemmpalreksis:FC-T2h0e3 3NMFPprwohdiucchtiiosnelqoutinvualmebnetrtowaFCs-n2o0t3pAr.ovCiudrerd.entThinefotersmtation 6in7d.i8ca5t%eswaittiesr,a 2m.ix6t6u%reSuolft1o.n3e4f%oaPmFeOr,S,3%25s%oddiiuetmhoyclteynlesgullyfcatoel,b0ut.y1l%etshoedr,ium lauryl sulfate, and 0.05% tolyltriazole.
cThhaerafcotlelroiwziendg scounmcmeantrryataipopnlsioesftiompaurmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the biodegradabilityofthe fluorochemical component of the test sample.
METHOD
Method: Modified OECD Screening Test, OECD 301E with DOC Analysis
Test type: Static acute
GYeLaPr:CNomopleted: 1984
Analytical Statistical
mmoentihtoodrsi:ng:ResDuilstssolwveerdeodregatneircmicnaerdbobny
(DOC) calculationofthe
%
DOC
rTeemsotvoarlgaanndisgmraspohiucrcient:erpAre5t0at:i5o0n.mix of soil extract and secondary effluent.
Tlihqeuosrescaomnpdlaerytaekfeflnufenrtowmaasn taheerastuipoenmbaatsainnattotfhaenMaecttrivoaWtaedstselwuadgteermixed
TfroeaamtgmaerndtePnlasnoti,lsSto.btPaaiunl,edMfNr,omwhRialemstheeysoainldwWaassahimnigxttuorneoCfofurnetsihe,s sinandy
Minnesota.
Test condition: DMuiislniunetgriaeolintnhwueatrtresiyren:ntthNemoteitdcgiviiuvteman:m.inNustorliuetnitonmoerdyieuamstpeerxtOraEctC. D 301E method wSetiogchkt/avnodlutmeestbsaosilsutbiyondipsrseoplvairnagtitohne:teSstolsuutbisontsanwceerienpOrEepCaDrendutornieant mcoendcieunmt.ratIinoitnisa:l csooncdeinutmrabteinoznosawteer2e0bmrogugDhOtCtIoLt,helifnoelalroawliknyglDbeOnCzene sulfonate (LAS) ~15 mg DOCIL, and the test substance ~40 mg DTeOsCtILv.essels: Not given.
Incubation conditions: Not given.
000972
Number of concentrations: one for test substance yeast extract, one `asnoddiounmebLenAzSoyaetaesyteeaxsttraecxtt,raacllt,inodnueplsiocdatieu.m benzoate vitamin solution, TTootsatlssuubsspteanndceedflsaoslkicdosnadnidtiponHs:onNdotaygoivfent.esting: Not given. Element Basis: Decrease in dissolved organic carbon.
Remarks: There is no mentionof blank controls, therefore it is questionable whether they were included in the study
RESULTS
pe-- er ----ree--------
Nominal concentrations: DOCIL and LAS at ~15 mg
Reference standards: sodium benzoate at DOCIL; test substance: ~ 40 mg DOCIL.
20
mg
Element values: 28-day degradation:
DDuupplliiccaattee 12 == 8967%..2%Mean value = 92%.
RSeubmsatraknsc:e RTeesmtairnkgswfaiseldc.onTdhuectveadluoensarempioxrttuerdeaapspldyestcortihbaetdmiinxttuhreeTaensdt not the fluorochemmical proportion alone.
ONCLUSIONS
The test substance % degradation based on the mean DOC removal was 92% after 28 days.
Tdahyes,rehfoewreevnecre,sutbhsetLaAncSe,onsloydoibutmaibneendz4oa3t%e,boyb2ta8idnaeyds9.5% degradation by 7
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
DATA QUALITY
Reliability: Kimisch ranking 3. of the method followed, as weil
Study asraw
lacks complete description and data records. The inoculum is
records not
ppurroipteyr,layncdhatrhaectsetruidzyedl.ackTsheanraelyitsincaolcchoanrfaicrtmeartiizoantoiofnotfhethaemtoeusnttsoufbstance
fluorochemical proportion in the solutions.
REFERENCES
The studies were conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 9611,1984.
000973
OTHER
_------
Last changed: 6/26/00
000974
Farmar a
TECHNICAL REPORT SUMMARY
To TECHNIGAL COMMUNICATIONS GENTER -- 202012
npr pr riodnoipos, st cos TO Guteoe
pie
Modified OECD
eening
an PC-203, PC-203A, and FC=00
SR
Foo3
None= See Environmental Lab Reg
96
z
Rsacum WoB ma--n -- Datum comunny |SEEe R p Deaonpo aoe LabCode J, ha __ .
To test the repeatability of the modified OECD Screening
Test in measuring the biodegradability of Light Water
a RETTe er
TSSS
ae
.|
L L
L
| !
Tthoisevasltuuadtye agtaheinbiusoeddegrtahedabmiodliiftiyedof OAEnCDtweSrcpreeLniignhgt WTaetster ~
products. The products studied in this set of tests were PC-203, FC-203 NFP (equivalent to FC-203A), and FC-600. Also tested was U.S. FC-203 Lot 3072. One of these products, FC-203 NFP, was also included in the previous set of tests done in 1980. The results of this testing were consistent with previous OECD Screening Test studies on Light Water products. The results indicate that all of the tested products are readily biodegradable.
Tn
D.R.Ricker-23p5-1B-10 D.W.Kent-236-RA-04
sm [itive ZL
5 000875
/-
2
:
MODIFIED OECD SCREENING TEST ON FC-203, FC-203A, AND FC-600
INTRODUCTION
LTihgihstsWtautdeyrwa(sL/Wu)ndperrotdaukcetns,toFdCe-6m0o0nstarnadteFC-t2h0e3,biousdienggradtahbei)lity of two tienstteirnngatwiaosnalrleyquersetceodgnzbiyed34MGoedrimfaineyd bOeEcCaDusSecroefeniinngcoTnessits(t!e)n.cy Tihnisthe results of past biodegradation tests done on these products.
MLoadbiofriaetdoryOEsChDowSecdreetnhiantg thTreestesLc/oWndpurcotdeudctsinwe1r9e80 hbiyghltyhebi3o4deEgnravdiarbolnem)e.psal TFhCe-20p6r,oduacndtsFCt-e3s0t1e7d w(eFrCe-20F6C-2f0r3eAeze(pprreovtieocutseldy).ideGnrteiafiteedr atshanFC-9200%3ofNEPt)h,e dtihesso1l9v80edteosrtgianngi.c cSaerebonTab(lDeOC)1 offortahesseumm3arpyrodofucttshesweasrerseumlotvse.d during
Table 1
Results of 1980 Testing on Light Water Products
Product
7
pry % Deg2ra1dation aptiDday: =
FFCc--220063 ,wep}
1233
9805
9534
9913
9923
HFey-d3r0o1q7u2inone
8397
9924
997
59a
9o35
Footnotes:
,1 FFCC--220033A.NFP is nonfreeze protected FC-203 and is equivalent to 2 HFCy-d3r0o1q7uiniosnefrweaezsethperotreecfteerdencFeC-2p0r6o.duct.
uTsheedsea19s8i0milraersulttesstcmoentthroadd,ictthethToOsCe DofegrH.adaHteilolnmanTnesatp'g),.D. MHuellllemra!nn)'swho
nTOoCrma(ltloytalobtoarignaendicrceasrublotns). teHsits freisnudlitnsg, ofhow8%eveTrO,C arreemovaanlexfcoerptFiCo-n301f7rom
canodntr6a8diTcOtC hriesmovoawln BfOorD,/ECCO-D206datian. theHis215--ddaayylobnigocThOemCicDaelgroaxdyagtieonndeTmeasntd
F(CBO-D3)017resaundltsFC-w2e0r6e, 5r0e8s%paencdti4v0e%lyo.f degradation for a 5-day BOD test.
tThehischeimsicaalsiogxnyigfeincandtemalnedvel(COoDf) of These opposite results within his
own paper clearly are an inexplicable discrepancy.
BHOeDl,lma(/nCnO'DsmeTaOsCurDeemgernatdsatmiaodneTebyst threesu3MltsEnvarieronamlesnotailncoLnasbiosrtaetnotrywiatndh oth8f" independent laboratories on 3M AFFF products. Such results
by
Ftoyrpiecxaalmlpyles,howa cBOoDl)f8ctviaolnuesof tsoucrhangdeatabeotnwee3nU.6S0.% maindlit9a0%ryOfspethceifiCcODa:tion
AFFF products manufactured by 3M is shown in Table 2.
000976
3
OPaEgCeD S2creening July 9, 1894
Table 2
BOD, Carbonaceous BOD, and COD Data on Mil-Spec AFFF Product
Lab
m5g0D/.R) c-(8m0g/Dxd} (mcg/okpg) BoCpo.B'
c-Bo, cop?
FC-203 C Lot 501
3M
580,000 600,000 774300,,000000 .79
81
Pace
800,000% 730,000* 783100,,000000
Csaeprscuole
547400,,000000 642400,,000000 975307,,000000 ..660
.l6e0s
Galibnroaciutlhum #1
616,000 671,000 776,-450 .79
`86
Galibnroaciutlhun #2
670,000 641,000 776,450 .86
.82
ASsv. eroba. ge
58755,,000000 59940,,000000 78820,0,000 00.1723
a725
* Calculated BOD's from different dilutions were very inconsistent.
FC-206 Lot 502
n SeParcceo CaGaplsburlaeith Galibnroaciutlhum #1
inoculum #2
290,000 290,000 336800,,000000 0.78 0.78 325400,,000000 236700,,000000 435700,,000000 00..7685 00.:8720 246106,,050000 342101,,000000 441007,,020500 01..6032 01.0718
395,500 374,000 407,250 .97
92
3 As.verDpa.2 ge 5,0730 ,000 0 33587,,000000 39"63,30,00000 1.86I
a8i3
FC-780B Lot 501
m SPearcceo Capsule sW.eroab.ge
240,000 230,000 333200,,000000 .74
7
225100,,000000 222500,,000000 325807,,000000 ..7713
77
240,000 230,000 360,000 .67
`64
23157,,000000 2"3123,,000000 32"92,800,0000.0I3T
"o0T5
00NsTY
OPEaCgeD S3 creening July 9, 1984
METHODS AND MATERIALS 1. Procedure
Tteessttiinngg wofascpheermifcoarlmsedadionptaecdcorMdaaync1e2, wi1t9h81t.he OECD Guidelines for 2. Chemicals
T(hCe;HorNeafOe,r)encFe.W.mat=er1i4a4l.s11 usaendd wlienreearlaablokryaltboreynzegnreadesulsfoodniautmebe(nLzAoSa).te, nU.dS.EEnAsvirSoonlmuetnitoanlusPerdotewacstioan5A.6g8e%ncyactasivea sreofleurteinocnesmuaptpelriieadlbyforthe wbeiroedegU.rSa.datFiCo-n203tesLtoitng3.072ThperovLiidgehdt Wiante1r983(Lb/Wy) thteestCommamteericailasl used CAnhtewmeircpalsinD9i/v8i2sioann,d FFCC--220033 aNnFdP pFrCo-v6i0d0edsabmypleEs.T.pSr.oLv.ideidn b1y980E..T.S.L. A(nntownefrrpeezperovpirdoetdectneod)lotisneuqmubeirvsalewnitthtotheFiCr-20s3aAm.ples. FC-203 NEP PtrheiorOECtoD ntuetsrtiinegn,t sthoelutLi/oWnanadndrberfoeuregnhcte tmoattehreiaflosllowweirnegdiisnsiotlivaled in mDgO/Cl,conacndentLr/aWtipornosd:uctssodaipupmroxbiemnaztoealtye 2400 mmgg//ll,. LATSheapLp/rWoTxOiCmawtaesly 15 ehxipgehreirentcheanhatshatshouwsned thiantprLe/vWioauts40temsgt/ilng is(20nomtg/t1o)xicb.ecauAslesoouhrigher concentrations improve the accuracy of the TOC analysis. sFoorurctehsisalsltuodwye,d bnyutrtiheentOECsDoluStciroenesninwgereTesptr,epayreeadstuseixntgracbtothandvitamin vsiytnatmhienticsouvricteamianffescotleudtiotnh.e exTtheisntwoasf dLo/nWedetgoraddeatteiromni.ne if the INocuLuM Tphreepairneodculiunmawcacsordaan5c0e/50wimtihxtOuErCeD opfrocseodiulreesx.tracTtheasnodils,ecownhdiacrhywaesffluent efxrtormacRtaemds,eywaasnd aWmaisxhtiunrgetonof Cofurensthi,es sainndyMinlnoeasmotgaa.rdenThesoislesconodbatrayined esfafmlpuleenttakseanmplferomwasan taheerastuipoenrnabtaasnitn oaft atnheaSctt.ivaPtaeuld sMleutdrgoeWmaisxteedwatleirquor TfrieltaetrmenptapePrlanptr.iorThteo cusoamgbei.ned inoculum was passed through Whatman 54 INSTRUMENT Tlhiemitorgiasniacpprcoaxribomnatealnyaly2zemrg oufsedcarwbaosnapeDrorhlmiatenr.DC-52A. Its sensitivity
000978
5 OPEaCgeD S4 creening July 9, 1984
SAMPLE HANDLING AND STORAGE
Tmhaenufsaacmtpulreerpreofsertvhaetioornganmiecthocdarbuosnedanwaalszytehra.t prescTrhiebemdethboyd thienvolves abdrdiinnggs t1 hedrospampoflescontcoenltersastetdhanHCpHpe2.r 10Themlsaofmplfeisltweerreed ssatmoprleed. undTehris risefrtihgateratthiisonstinoravgiealsmetwhiotdh acalnumpirneusmerfvoeilL/lWinecdontcaapisn.ingOusrampelxepserifeonrce fservoemratlhismonstthusdywiwtehreoutdonaeffewcittihnign otnhee wDeOCe.k ofAllitsDOCcoamnplaeltyisoens.on samples
RESULTS AND DISCUSSION
Tsaobdlieum3besnuzmomaatrei,zesthethererfeesruelntcse ocfomptohuinsd,stuwdays..neaTrhliys ctoamblpeletsehlowysdetghraatded g(r9e5%a)terwitthhiann 780%dayTsO.C reEmvoevraylrweiptlhiicnatetheof2a8l-ldayof tetshte Lpe/rWiopdr.oducStesvenshoofwed tthhaen t9e0n%teTsOtC rfelmaosvkasl.contThaeinianvgeratghee vDaOCrioruesmovLa/lW pfroorduacllts fosuhroweLd/Wgrperaotdeucrt was greater than 90%.
TThheeseresruelstusltsofartehisavetreasgteidn,g aanrde sbheocwanusegroafphitchiasl,lydoinnoFtigusrheosw t1heandact2u.al
epxaatmtpelren gorfapdhegroafda2ticounltuirnesthedegirnaddiivnigduatlhecuslatmuerepsr.oducFti,gurFeC-2303isNFaPn. It
rsahpoiwds, tbhuatt otnhcaet dtehegraladgatiporniorof tothitshepornosduecttofbedgaengratdhaattioint wwaass vvaerriyable.
kThneownrenaosronwafsorittheisxpevcatreida.biliIttymabyetwheaevne bteheendduupelictaotetheculftacutresthawtasthneot
dcuelgtruardeisng rtehceeimvaedjordifcfoemrpeonntentlsevoefls thoef
vLiiaghbtleWmaitecrrooprrogdauncitssm.s
capable of Thus, the
cpuolptuulraetsionprioforbatcotetrhieaonwsietth otfhesreapicdapa(beixlpiotnieenstiahla)d dteogrbaudialtdiounp. in the
RteevsiteiwngOfofthtehedoattaherinLT/aWblperod3ucitnsdicaaltseosvatrhiaetdrcesounlstisdeorfablryepalticdaatyes 7, l14e,ngtahndof21.theAglaaign,phatsheisprviaorriattioontheappoenasrest tofo bbeioddeuegrapdraitmiaorni.ly tHoowetvheer, hduoew ltoongthebiosdmealglradnautmiboenr ooff sianmdpilveisduatlakeLn/,W conoentafirneiqnugentcluyltcuoruelsdtonookt tell following the initial lag.
000979
6
OPEaCgeD 5Screening July 3, 1984
Table 3 Biodegradation of LIGHT WATER Products and Refernce Materials
Product/vitamin Source
sSooddiiuumm BBeennzzooaattee//Yyeeaasstt--21,(,1) SSooddiiuumm BBeennzzooaattee//VViitt..--12
Mean rc-2032) /veast-1 FFCC--220033//YVeiats.t--12 FC-M2e0a3n/Vit.-2 UU..SS.. FFCC--220033//YYeeaasstt--12
Mean FrCc--220033AA(/5Y)ea/svte-a2st-1
Mean FFCC--6600003//Yyeeaasstt--21
Mean LLAASS//YYeeaasstt--12
Mean
Im8 DOCunRemovalaAfterzmX Days =m
99s9..86 9848..46 95
1210.07s 2390.s1 ----(4) 2287..40 9glais0 9925..13 22940148 8382.066 ----- - 9207..27 8950..65 8953..26
92
2664..93 2950..18 ----
7990..27 9965..05 9976..55 97
1123.26 88.551 ----
8151..10 9826..54 9867..02 92
76.76 1750.055 ----
7--6=.(36) 8g7o..03 8084.28 91
25.25.8 78..88
2250..14 -3-180) .8 24091.71 35320.1% 3
Footnotes: {(33)) uUsseedd OyeEaCsDtseyxnttrheatcitcasvitvaimtianminsolsuoturicoen. as vitamin source.
{3} (4)
1Pr7o-mdayAntswaemrpples15w62e.re
only
taken
for
LAS.
.
(2) FSraommpleAntcwoenrtpami1n9a8t0e.d, Orniotginuasledl.y labelled FC-203 NFP.
000950
READY BIODEGRADABILITY: Modified Screening Test
EnvOiErCoDnm3e0n1tEal--LaAbdoptReeqdues1t2 MNoa.y 891611
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Figure 2
READY BIODEGRADABILITY: Modified Screening Test
OECD 301E -- Adopted 12 May 81
Environmental LabRequest No. 9611
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{ OPEaCgeD 9Screening July 9, 1984
Tshuebsmtaondciefsi.ed OLE/CWDprSocdruecetnsi,ngonTestthe isothienrtenhdaendd, foarre usaequeonoussinsgolleuticohnesmical ctohnantai7n08inglosasmioxftuDrOeC owfithcihnemi2c8aldsa.ys, Paurree rcheegmaircdaelds,bywhtihcehOEhCaDve greater grueiacdheeldinewsithaisnbe10ingdayrseaodfilythebioDdOeCgrraedmaobvlael. fiArlssto,excteheidsinrgesu1l0%t. hasOnltyo be soneeconodf OtEheCDrreepqliuciarteemsentofofeac7h0%ofdegtrhaedaftoiuornLw/iWthpirnodu1c0tsdaytsesotfed fmiertst this rreeaaclhiisntgica1l0%lydaepgrpaldiactaibolne. toThLi/sW orreqoutihreemrenfto,rmuhloawetveedr,proidsucntost d(imfifxetruirensg).suscTheepticbhielmiitciaelss wtiothbiinodemgirxatduarteisonw.illRefardeiqluyentdleygrahdaaveble wchheimliecatlhecoonmspeotnenotfsdoefgrasducahtiopnrodoufctosthemraychsetmairtcaltso diengrtahdeese ipmrmoedduicattselym,ay rbeeadseolnaaybelde. forThmusi,xtudriessregofardcihnegmictahliss, stehceondrecsruilttesrisah,owwhtihcath tihse nmoitxture bofiodcehgermaidcaabllec.omponents in each of these products is readily aCulstluirgehstlytesltoewderwiltevheltheofsyDnOCtherteimcovevditatmhiann csuolltuutrieosn ushsoiwnegd,yeaosntaevexrtargaec,t yaesasttheexvtirtaacmtinsisnocuercet.he sFuytnutrheetiOcECDvitsacmrienenisnogluttieosntsisshmoourled dtihfusficuuselt to prepare and does not give improved degradation. dLeASt,ergwehnitcsh, idsida nroetaddielgyrabdieodefgurlalydabulnederandthecsoemmotnelsyt cusoendditsiuornfsa.ctanTthisin OofccuDrOrCedcomipnarsepditetoofthethe40 fmagc/tl tohfatDOiCtsusceodncefnortrattheionLigwhats Woantlyer15 mg/l ptroodbuecttsh.at TLhAeScirneahsiobnitefdormitchreobsilaolw rdaetgeradofatiLoAnS batiodtehegratdeasttion appears hctaoelsnftcepwneetrrreiaotddi,iolnuttheed'. retTmooaitnwciihncegeck LtAhtSehiirsconvhtyoapliountmiheensgwiistt,hestfatrceustlhhteuirenenosdcuwloefarteetdhesnpul2it8tr-ideaanyntd mtieindmcierae.aofsedAdtilsututhbiissotnanptwoiaiasnltl,rye.mtohveeSdreavwteeinttyhoifnpdeer1cg5ernadtdayasto.ifonthReeisnuDlOttChse rodefimlauittnheiindsg wcouartlktutrohnees sLiASmilaarrelyshorwenporgtreadphiacallolwyleivnelFiogfurebio4.degrAandoatthieornroefseaLrAcSheirn, thheasmodified sOuElCfDonsactree)enihnagd ateshta.lf lD.ifeLiuof fo39unddatyhsa.f arlon A (dodecylbenzene CONCLUSTONS
TTheestsproensenLtighsttuWdaiteesrrperpoedautcetds tdhoenefibnydintghse o3fM pEnrveivrioonumsenOtEaClD SLcarbeoernaitonrgy. bBiotohdegsreatsdabolfe.t"estsInsahlolwedcastehsa,t Lth/eW apvreordaugcetsDOaCre re"mroevaadlilyfor L/W products dwaesgragdraebalteerthtahnan th9e0%vearfytercom28mondlayys.usedL/Wandpro"druecatdsilywerbeiomduecghradmaobrlee" surfactant, LAS.
.
000985
j n : "OEPaCgDe S10creening July 9, 1984 REFERENCES 1) oFrorganTiezsatitnigonofforCheEmciocnaolmsi,c CSoeocpteironati3o0n1 aE,ndReDaedvyeloBpimoednetg,radGaubiidleiltiyn:es Modified OECD Screening Test, May 12, 1981. (2) g6,/80a,. R3eMinTeerc,hniBciaoldegRreapdorattioNno. of40,"LDIeGpHtT. WA0T5E3R5," PPrroojdeucctts99in700O1E2C6D00T,est 6/30/80. (3) Ebrx.tinH.guiHsehlilnmgannFoaamndCoD.mpMouulnldesr,(Um"wEenlvtigreofnamherndtuanlg Idmupraccht of Fire IWPeesunterGsieorstmcahni-gethauunntitoetfell.H")ydrTohS leogy t (Buwnu daessaCnd asrtraily etdfouurt by the gewasserkunde), April 18, 1979. (4) pTbe.stmulOlregraniacndMTa.terTiiatltsizefro,r C"oTmhepleTtOeC BDeigordaedgartaidoantioTnest(D-erATOPCroc-ess to `sAubfbauVotlestlst~anediingeVnerBfiaohlroegnTaczhuernPrufuAnbgbau)~ vonDeOvreglaonpbeidschaetnthSeubstanozeon ASbwu assrer-dForescfs hounragGe(wn1a9s7ss9e)r.tkua ndsl, Ktoblenz, 7. P. Waser-und (5) ponrman DC-52a Operating Manual, ath Ed., 1978. (6) MRra. rcp.elSwDieskhkeerr,, SIuncr.f,act1a9n70t% Biodegradation, pages 16-17 and 147, (7pB.iodLeiut,eriSotruadtyioonn 5B,iodEedigtreaddatbiyonT. ofA. AnOixlleiyneanadndS.MarBlaornrryA,, 1983.
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