Document ymX6a4dOVNxX7mqmyO8bR5gmD

Younger Laboratories BiocUomiiii... PhaMMcolaqidil... Analftti. Route 9 -- Box 281 ST. LOUIS 23. MISSOURI RHONKt TlLOlN 6-2540 Oetobor 20th, 1958 OTJXCT - Toalooloclaal Bnrootifatloa Of OB-95. Raninto SMpls Mmbar 102 - Lot 7501-3677; Won--to flMplo ltatov 17 - lot 8-59 feosmto Projoot Mater 1-58-29 IBIS CCnOCRD 99 teaate ("teal ml flapmr, at* Unis, HUmui nmamau, iwonox - - A) aids Abaarptton JOS (tebhlto) Ite awrttlutad soopoond vao appllad la Taryin* Mounts to tte alooaljr silpp-d. latest tela of albino rabbita ad tte anlaala plaead la uoodaa atoeks for slt tears after white tte tnafc mss wrappad la plaatle (trip* ad tte mlaals rrtumad to lndltidMl oafao* OtaorratloM wars aada far taxis syaptaas md tte vlaearo of solaotad mteala frea ttesa aoeoaibdLac was Mated aaoxoseopleally* Tte date an teown la teUo X. B) Slda Irritation (Rabbits) 0.1 MUUllter of oailluted 08-95 was tppliad to tho olippad, intact (kin of albino rabbits and otearratlona aada ovar a parlod of several daps for Irritation* Tte results, aoorad aeeordlnf to Draito, Woodard aid Calvary (Journal of Pham, and Sep* Ttempsutlea, Voluaa 82* 377 (l?id)), am ahem in Tabla II. hwmoer umutcbies hons 038112 To* rcnsanto Chemical l. .panr, St. Louis, Missouri lounger Uborstoiiss Certificate of Analysis - Page 2 (10/20/58) - T-58-2J TABU I rancxrr o? os-95' bt sox abscrfticm IX RABBITS Semple Applied Ubditoted Height Dom Animal No. - Sax I* . Ng./It. 3AWIS 1C2, tot 7501-3677 1 - Kelt 2 - tosmle 3 - Psrnsle 2.1 1.8 2.0 b Mala 5 - MBla ' 6 - Mala 7 - tomato 2.b 2.3 2.b 2.0 1000 1500 2000 2000 2500 2500 3500 SUUfflS 17* Lot 3 1 - tomato 2 - Mato 3 - Mala b - Mato 2.3 1000 2.6 1250 2.5 1250 2.3 1500 5 - tomato 2.2 1500 6 - tomato 2.1 2000 7 - Mala 2.6 2500 Weight (Sunge 5 Daya totar _... **. ... 0.0 - 0.2 - 0.J - 0.1 - 0.2 - 0.1 - 0.J - 0.1 0.0 - 0.2 - 0.3 ' - 0.2 - 0.2 - 0.3 fata Survived Survived Died - 7 Daya Survived Died 6 Daya Dtod - lb Daya Died - 5 Daya Survived Survived Died - 17 Days Died - 9 Days Died - 7 toys Diod > 7 Days Died - 6 Cays DISCUSSION - Thsre was on* death and ana survival at 2000 mllligrama par kilogram on Sanpls 102, tot 7501-3677. Survival tlm raiged from firs to fourtesn days. Gradual loss of appstlta with laanasing lethargy and waateteaa developed. Co lanpla 17, tot 3-59 than was ons daath and ana survival at 1250 islUigtsms par kilogram. Survival tins was six to savantaan days. Toxlo symptoms wars very similar to those already described. At autopsy thsro appeared to ba degenerative cluuigos in the liver by macroscopic examination. Pulmonary hyperemia was also obsemid. MONS 038113 Tar Monsanto Ctamlcal &. yany, St* Leals, Missouri Young*r laboratories Certificate of Analysis - Pag* 3 (10/20/38) - r-58-29 TABLE H sxa xruxaizcr sr rabbit after application op >08-?$' temple Applied tfadllubed Animal Nutter 1 Hoar SAtKE 102, Lot 7501-3677 10 20 30 Average - 0.0 SAVUE 17, LOT S-59 1 2 3 Anrags 0 0 0 Q.0 Nteerleal Evaluation At Qia End QC It Hours 2it Hours US Hour* 72 Hour* 0 2 1 1 13 21 122 1 0.6 2.3 1.6 1.0 13 2 2 12 2 1 13 2 1 1.0 2.6 2.0 1.3 120 Hours 0 0 0 0.0 1 0 0 0.3 DISCUSSION - No irritation was obterred on titter temple on* boor after exposure. Two out of tbre* animals on saapla 108 stewed haroly peresptibls srythsn* after four hour* for an seerag* soor* of 0.6 out of a possible 8.0. Or*might there was well dsflasd etythsas with one instancs of slight edena Increasing the average seem to 1.3. teaoral of the application reduced the irritation to barely peresptibls redness on tbs third day. filer* was practically no diffx*ae* tetwesu tb* two sanples as tbs data in tbs . table lndieat*. The average twirty-four hour score for matter 17 was 2.6 as compared to 2.3 for master 102. The two sample* of 06 - 95 were olassed as mild to noderate sJdn irritants. Ti'-> ntji -i,| ;r (hi, rPT,rrt (s to b. '* Pt OR.:! ukH-v,, I>t'. it i.t "... lx I;..!n1 j , . ' "i'-- r '' ; . ,,, . ,, ifl I........ . u . . - * 1 ' * >*f .-x :-r. I'M. it.\1 : r . . 1 l.- ;,. .. : is.. . i i. r* . L' hows 038114 Tat Monsanto Cbtnical C panjr, St. Louis, Missouri Icmft Laboiatariss Cartlfleata of Analysis - Fags U (10/2Q/S8) - T-S8-29 aamm -- Hm Minima Lothal Doss rang* by shin absorption in mbbita was datanalnad on tm aaaplas of OB - ?5 ' Maabor 102, Lot 7501-3677 gars a tangs of 2000 to 2500 nillicnH par kUogtan. Htabsr 17, Lot 5-59 gars a nogs of 1250 to 1500 nillicrans par Jdlogzan. Both aanplaa vara olaaaad as aild to aodorata alda irritants. Ifco avarags aaxtmai scara for maibar 102 was 2.J oat of a poaaibla 0.0 In tnonty-four hours. Ntanbar 17 aeorad an arazaga of 2.6 aftar tha sans tins lntorral. ' MQNS 0381A5