Document ymOZqDzE5akVVMz2X3YmVv67r
5A-to c^clmti^ic cd-Mociate*
1474 S.Vakdzvsntbk Avs. . St. Louis 10, Mo.
February 1, 1955
CERTIFICATE OF ANALYSIS
SUBJECT! Toxioological Investigation of pydraul F-9 (FH-103)
TE'TS CONDUCTED FORi Monsanto Chemical Company, Monsanto Project No, SA-90
St. Louis, Missouri.
'
(f 10 5*
EXPERIMENTAL PROCEDUREi
^------------- !
A) Aouta Oral Toxidty
" '^X
The oral LD,0 was found by the introduction _of measured single doses of un
diluted compound into the stomachs of Sprsge-pa*le7 strain Albino rats by
sans of a rubber catheter attached to a hypodermic syringe*
After the approximate Miniarum Lethal Dose was determined, groups of- rats were
fed at levels designed to blanket the toxicity range thereby supplying data
for calculation of the LDg0*
./
Selected animals from those succumbing were fiutopsied and maoroaooplc eiarl-
nation made of the viscera. ^
The data are show In Table j:.'
.
B) Toxicity By Skin Absorption
.
Undiluted Pydraul F-9 (FH-103) was applied to the closely clipped intact skin of albino rabbits in varyii^ amounts in /order to find the Minimum Lethal Dose.
The treated area vai^vere&with aplastic shield to retard evaporation and
the animals were^laoed'in special -edges to prevent them from licking off the
mpu.
jr
The remit, tth found In T4hJ.e XI. C) Skin IrrUtldn\In Rabbit. ' j
The coring metmd of Dr^i^e, 'Woodard and Calvery (J. of Pham, and Exp. Therapeutics, Vol^Bfij-Moe' 2, Deo. 19Ui) was used in evaluating Pydraul F-9
(FH-103) as a skin irritant whan placed on intact rabbit skin.
Observations mads over a period of several days are aoored in Table III.
D) Eye Irritation In Rabbits Undiluted compound In the amount of 0.1 ml. was placed in the conjunctival sac of the right eye of each of three albino rabbits end the degree of irritation aoored aocording to Praise, Woodard and Calvary (J.. of Pharm. and Exp. Therapeutics, Vol. B2, No. 2, Deo. 19J40*
The results are given in Table IV.
TWi m fwidunl report which mf ba urad whao raquaatad hr pbywdaa* aad baahh ofidala, but ii doc to ba wad la aay fin of adwtUm without arrina pawOduu.
CONIULTANTI TO THI FOOD, CHBMICAL AND fH A1U ACIUTIC AL
0 16682* NDUSTIIBS
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Ti iMMtto Gfataiaal Do*. Jt, St. Lowia, Maaouti.
Solontlflo Anonel at** Qirtifloat# of Analysis . Pag* 2, t-1-55
CTPSHIKESf/U. PROCEEDRE l (tootimed)
E) Vapor Inhalation Fonr Mature Mala rat* nor* plaood In a anall natal drun and exposed for alx houre to a aatnratad atMoaphere of Pydranl F-9 fmo* by pasalng a otroaM of oOMpMOsod air through a oontalnor of the fluid plaood Inside the oha^or. Air proasure was 16 pounda per square inch. The animal* ware ohookad for y*pto*a of toxlodty and all a*re held for aoron day* obooraatlon following axpoaur*. Th* data ar* ahoMi In Tabl* V.
stnmiKTi
Tba Oral LD*0 of Pydreul P-9 (FH-103) for rata waa found t kilograM.
yzh.O grana par
The malaiM Lethal Coaa by akin abaorptlon In rahb: kilogrMi.
Slight rednaea with a traoa of edaaa for an areraga aoore of akin applloatlon.
Eye irritation nountad to Moderate ndnea MarlMim aoore of 12.3 In 30 Mlnutas. The
t edena for ad axerag* net nra hardly affected.
Ho death* resulted when rata were expos for six hours.
d atmosphere of Pydraul F-9
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To i Monsanto Chndoal Co 1117, St. Loo 1, Klaaouri.
Selsntifla Aeeooiatsf Oartifioate of Analysis - Page 3, 2-1*5$
niLi 1 THi ORAL LP,0 OF PTDRADL F-9 (FH-IOJ) FOR LA30RATORT RATS
Animal No* - Sex
Weight Ge.
Dose Offl. / Kg.
Fate
a- Male
260
16.0
Survived
2- Hale
275
16.0
Survived
V3- Ft-'ale
215
16.0
Survived
km Kale
21*0
16.0 /
Died
$- Kale
230 18.0 \ \ Survived
6- Female 220 21.0 \ \ Died
7- Female 21*5 21*0 \ \ Survived
S- Hale
250
\ Died
9* Female
195
Survived
10- Kale
220 2l7o^-- Sui^ived
11- Kale
25C __ _ 21*. 0
Ntarvi^ed
12- Kale
230 / %2U.O
13- Female
210/ /N.
lit- Female 15- Female 16- Kale 17- Hale
W / 721* Jo
&> <Ks \ XV-`2l0\ NS /*7.5
IS- Male
259 \ < 27.5
19- Male
^\2l*5 '\ Nz-5
20- Male / .
21- Hale / / ~V6\
\ 2\5
\?/;5
Died Survived Survived
Died Died
Died Died Survived Died
\
EISCOSSIOHt
V\
\j
The Oral LDB0 waaplaoed at Sl^.O'gramv'per/TCilofrar. SorviTal time vaa one to
eight days idtlydeveite diarrhoVnresent in all animals. In all probability, part of the dome an ranted relatively unchanged due to the combination of diarrhea antytdgt>7iohage.y. Appetita~*a affeoted but aside from the gastro intestinal diatdrhanoea > other symptoms of toxioity were observed.
At autopsy the Visoera apjtears'd normal exsept ibr irritation of the digestive
tract.
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To; Monsanto Cbondetl Coapany,
St. Louis* Missouri. Solentifie Associates Oertlfioate of Analysis - Page k9 2-1-55
TABLE II
THE HINIMOH LETHAL DOSE OF PYDRAUL F-9 (FH-103)
BT SKIM ABSORPTION
Weight Dose
Weight Change 5 Days After
Dosing
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Tot Itonssnto Chewloal Cor ly,
St. Lffdfj Missouri, 3ol*ntlflo Associates Certificate of Analysis - Page 5, 2-1-55
TABLE in
SKIM IRSITATIOK IK 8AB8ITS AFTER APPLICATION OF PTWAOL F-9 (FH-103)
Animal Mo, - Sex
1 - Female 2 Kale 3 - Female Ararage
Numerical evaluation At The End Of u30 Min* ^24 Jfours 46 Hours 72 Hours
2 1 2 1,6
DISCUSSION i
Slight redness with practically no edema ms preseg
average aaore of 1,6 out of a possible 6,0, The
and ms due to a slight increase in redness with a
third day the staple bad newly disappeared from the surfai
the average score bad reduced to 1,6,
'
Pydraul F-9 (FH-103) ms considered to be/4
3sj irritant.
DISCO SSIONt
The palpebral ool thirty ninutes for' cornea sere hardly a' appeared to be normal
moderately red and showed a trace of edema within 'eoore of 12,3 out of a possible 110, The irie end
The boore dropped to 9*3 overnight and all eyes hours.
0 36bd^3
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St* Ranaaato Cheadeal Omr*ny, at. Leala, Hlaeourl.
amiimtUU iintlltoi Oartlfleat* of Anaiysla - Pag* 6, 2-1-55
TABLE I
VAPOR INHAIATIC* 0- PTTWACL F-9 (FH-103) BT RATS
Animal No. - Sex
rate
Concentration Of Pydraul P 9
Vapors I n Cbanber
Average Temperature
I aside Chamber
Average Relative Humidity
I n aide Chamber
1 MO* 2 - Rale 3 Rale It - Hale
SurviTad
Survived Survived Survived
Saturated Ateosphere
76.5'F.
do.01
DISCOSSIOKI
The vaporised Pydraul ?~9 aettled quite rapidly on the ohaabar valla. Qoular and nasal Irritation^**? moderate, heavy and irregular. Lethargy developed toa moderate extent expoanre began.
rula and the boom fairly after
The animals ear* quite axhauatad ehan Jfakep^out'hf tie ehmber but all had re gained normal activity and appetite U^thil^lyurs^follnving exposure. There
vara no deaths either during or folio
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