Document ykQyggd13qyXJdDY5m2DbaXV3

CRI NUMBER R & D REPORT cm NUMSER K- 00 l m- DOW CHEMICAL U.S.A. RESTRICTED: for u** within The Dow Chemical Company only* DEPARTMENT LABORATORY REPORT CODE ES-187 DATE f*U CO Novem ber 18, 1977 LAB. NO. Problem no. Environmental Sciences Research TITLE h 9, 1 UMHl TOXICITY OF 1,2-DIBROMO-3-CHLOROPROPANE TO DAPHNIDS 7 PAGES IN FULL REPORT AUTHOR (S) W. M. McCarty UTHOfi I5i SIGNATURE IS) VIEWER'S SIGNATURE V DESCRIPTIVE SUMMARY WITH CONCLUSIONS: This report interim ___ j5T| FINAL and mainly: NEW j I REVIEW (Include in this ipoce references to data books, end to earlier related reports, patents and publications.) 1,2-Dibromo-3-chloropropane was evaluated for acute toxicity to an aquatic invertebrate species, Daphnia magna Straus, using a static water test method. The 48-hour LC 50 for the daphnids was 24 (22-26)* mg/i. *95% Confidence Interval distribution DEPARTMENT FILES R & D ADMINISTRATION CENTRAL REPORT INDEX **.*>*. BMc, - M .rf Iff" J' COPIES DO 13198F. CONFTOFNTTAl D * ft tr i but ion 11 > f I continued on attached oooe. INTRODUCTION I,2-Dibromo-3-chloropropane, a nematicide, was evaluated for acute toxicity to an aquatic invertebrate, Daphnia magna Straus, using a static water test method. The acute daphnid toxicity was run for the purpose of pesticide registration. MATERIALS & METHODS Pesticide The sample of 1,2-dibromo-3-chloropropane used for this acute toxicity test was identified as AGR 133748 with an assay of 98.2%. Test solutions were prepared on an as received basis. The test solution was prepared in dechlorinated Lake Huron water. Onehalf ml/i acetone was added to the test solution for better dissolution of the compound. Water Water used in the acute toxicity test was dechlorinated Lake Huron water. This water exhibits chemical characteristics in the following ranges: dissolved oxygen (D.0.)a, 7.7 mg/A; pH 7.6^; total alkalinity, 85 mg/l as CaCO^; total hardness, Yellow Springs Instruments Model 54 - Oxygen Meter ^Sargent-Welch pH Meter - Model LS 00 1919RR OONF TDFNTTAL -2- 100 mg/l as CaCOy specific conductivity, 1770 ymhos/cm; and resistivityc , ^570 ohms/cm. Other water data are in Table I 1 . jThe static water acute toxicity test follows the test method described in the EPA publication, "Methods for Acute Toxicity 2 Tests with Fish, Macroinvertebrates, and Amphibians" . Daphnids The static acute toxicity test method consisted of exposing first instar daphnids reared in our laboratory to various concentrations of the material being tested in dechlorinated Lake Huron water at room temperature (25C) . A stock solution was prepared in dechlorinated Lake Huron water with 0.5 j^l/l of acetone added. The concentration of the stock solution was 0.1 mg/m. Stock solutions older than 48 hours were discarded. The required amount of stock solution was combined with sufficient dechlorinated Lake Huron water to make a final volume of 200 mi. in the 250 mi test beakers. A control was set using dechlorinated Lake Huron water. Because acetone was used in the stock solution, a second series of beakers were exposed containing the largest c Yellow Springs Instruments Model 31 - Conductivity Bridge 00 13T987 OONFT DFN1 T Al TABLE I RAW LAKE HURON WATER ANALYSES Turbidity, as ppm Si02 <1 Color, APHA *<5 Alkylbenzene sulfonate, ppm <0.5 Arsenic, ppm >0.005 Barium, ppm 0.008 Cadmium, ppm **nd(0.01) Chlorine, ppm 7.0 Chromium, ppm Copper, ppm nd(0.005) 0.02 Cyanide, ppm nd (0.01) Fluoride, ppm nd (0.5) Iron, ppm 0.02 Lead, ppm nd (0.01) Magnesium, ppm 7.0 Manganese, ppm nd(0.001) Nitrate, ppm nd (3) Phenols, ppm nd(0.001) Selenium, ppm nd(0.01) Silver, ppm nd(0.01) Sulfate, ppm 16 Total dissolved solids, ppm 116 Zinc, ppm PCB's, ppm <0.05 nd(0.101 x 10~3) Mercury, ppb nd(2) DC iMCbC C.ONF T DF NT T At *< - indicates detected, but at a value less than shown **nd - is not detected, followed by minimum detectable amount -3- amount of solvent used in any concentration to act as a solvent control. The concentration of solvent in any beaker did not exceed 0.5 mSL/&. Ten first instar daphnids were then added to each beaker. Three beakers were used for each exposure concen tration and each control. The standard exposure time was 48 hours. Death was defined as no response to a gentle prodding. Dead daphnids were not removed from any beaker during the test. Statistical Calculations For each set of mortality data, the LC values and their 95% con fidence intervals are given. The LC 50 is the estimated concen tration of the test material at which 50% of the test organisms are dead in a specified time interval. This value is determined using Finney's probit analysis method3 with a computer program. RESULTS The 24 and 48-hour LC 50 values and their 95% confidence intervals were determined on Daphnia magna. The 24-hour data should not be used because of significant lack of fit for the probit model. 1,2-Dibromo-3-chloropropane exhibited a 48-hour LC 50 value of 24 (22-26) mg/S. to the daphnids. Concentrations tested and mortality data are shown in Table II. The control daphnids exhibited a 10% mortality during the test. ( 'OfSlf T r,r TABLE II THE TOXICITY OF 1,2-DIBRQMO-3-CHLOROPROPANE TO DAPHNIA MAGNA AND STATISTICAL DATA o J : jr j- j-CHL'l i. i 'R h U l 0/ L OmIM 9 I A V A 51, 24 HR. 0-' .i I T jI./'U < V ! "t , r ) = 2.73. 95* C. i.=( 1.74 : i - j . i,`. r j; l a!. *. `-ir l i. i T j i / , A i J ( i.i. .'! * - . Ji 1 I 1 .11 .1 I T i 9. t ; *- uGoI'LEj OF T _ , :J - . . . - n 1 * . S *-* L- A b 1 Cj'^FUK ATI U '* L ** ` * 7 ' i ' i - 0 .2 . ) MO 1.0; 1. 7 1. i . r-j 1 . 3o . , jf J '. r J4 /. t V ' Jli ^ * " ;i 'j! T ir' / 3. ^ ^ 4 57 ,, 5. 6."' 3 P . 32 4 6. 2``3 bXr'. ph;^it 3. 7i5 4.2 j C 4.7^3 4.94 3 5.234 5.631 l_ . . , 11 , . \ 1 !- L 11 _ L ^ I * J [' P L r L I i I4* y ' * .y i i -, f * j 2.j.l7 43. j3 ' 1 , O1 m 1 . M 2 i 3 t 4 1 i- - 0 i . < u ' j - $ - L I L l)Jr* A . l u/ L :j A 15h'J 1 A ^ A A ho mR * l( *)-- ^ *t.j 9 5.1, G * I . = ( 5 0 3 1 J )i ) -- " J L J1 \ v- I r i L a! V A L uc ( ^ L ' I - * ^ 1 0 1 4.37 ..ITH * OGGREEo OF FRCLDo 5.9 > 'l *_ ~ k ^ >0 _< i o , * V * t + * 3 i i. *> C C j \ l. G .'J T ft A1 I J \ L,. . 1 . L- L 2 4 . 0J . ;' '' * L JO ( CU' 1 .2b 1.33 i 1.7 1 i2 r\ j ' j k j J!.}. f'KJbll 4.272 4.6 6 U b.634 LXR. PKJ^II 3.-77? 6.6*17 7.094 1I I 1 L > ' L I I. b 1 Mi *1 j J 1. '1 l C.I 1 . u* 3 3. L j 39. .3 1 31990 DO lAl -4REFERENCES 1. Hunemorder, E. J., et al. 1977. Dow Report ML AL 77-50097. 2. Ccmmittee on Methods for Toxicity with Aquatic Organisms. 1975; Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians. Ecological Research Series, EPA-660/3-75-009. 3. Finney, D. J. 1952. Statistical methods in biological assay. Cambridge University Press. DO 131991 CONF TDFNT T Al