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AR226-2918 FOR DU PONT USE ONLY E. I . du Pont de Nemours and Co., Inc. Haskell Laboratory fo r Toxicology and In d u s tria l Medicine Elkton Road, P. 0. Box 50, Newark, Delaware 19714 HASKELL LABORATORY REPORT NO. 114-84 MR NO.^ i - O O l ^ SUMMARY: >1:CD rats were aerosol atmospheres. i f either S r ^ T n g l e ^ AivALuToi _ ____ _ li_s__ mg/nr of s o lf f S . An ALC f o r j B P B B f T ^ 49mg/m oT"solids. According to Haskell Laboratory Acute T o x ic ity C lasSTficatio; , both m aterials are extremely to x ic by in h a la tio n . INTRODUCTION: fore, was tested as a p o s itiv e contro inhalation .ontains There- I I . PROCEDURES: A. Animal Husbandry: Young adult male Crl:CD ra ts were'received from Charles River"Breeding Laboratories, Kingston, New York. Rats were housed s in g ly in e ith e r 5 " x ll" x 7 " o r 8 "xl3 "x8 " suspended, s te e lmesh cages in rooms maintained a t 50 + 20% re la tiv e hum idity and 56-75F on a 12/12 hour lig h t/d a rk c y c le . Each ra t was assigned a unique id e n tific a tio n number which corresponded to a number card on the outside o f the cage, and r a ts ' t a il s were color-coded during exposure. Except during exposures, Purina C e rtifie d Rodent Chow #5002 and water were available ad lib itu m . Rats were quarantined f o r 1 week p r io r to te s tin g . Rats were weighed and observed twice during the quarantine period. " 1 " Company Sanitized. Does not contain TSOA CM \ P ro to co l: Groups of 6 ra ts , 8 weeks old and weighing between 234-293 grams, were restrained in perfora ted , s ta in le s s ste e l cylin d e rs w ith conical nose pieces. Each group was exposed n o s e - o a ^ ^ g ^ ^ ^ ^ ^ te ^ 4 - h o u i^ e r t o d to an aerosol atmosphere of a i r . S u rviving ra ts were weighed and observed d a ily fo r 14~days post exposure, weekends included when deemed necessary. C. Test M a te ria ls: Synonyms: Other Codes: S ta b ility : Test m ate rial is assumed to be s ta b le throughout ^ the exposure phase. Submitted by;__ T e x tile Mbers Department Seaford 2) 3% TBCU-AS: TBCU-AS Composition: Test Mixture Contaminants Synonyms: Other Codes: S ta b ility : Submitted By: Jackson Laboratory tiOinpaRjf Sanitised, ioes nst eonfsist t SC CSV * D. Generation: Liq u id te s t m aterial was pumped in to a Spraying Systems* N ebulizer. A ir Introduced a t the nebulizer aerosolized the te s t m a te ria l arid swept the aerosol stream through a cyclone e lu t r la t o r . The cyclone removed large p a rtic le s by I n e r tia l impaction w h ile aerodynamic p a rtic le s passed through the cyclone and In to the exposure chamber. For t h r e e M ^ I B B w x p o s u r e s , the a ir stream was hum id ified p r io r to e n te rin g the n e flffliz e r in an attempt to increase p a r t ic le size by m inim izing water evaporation. E. A n a ly tic a l: At approxim ately 30-minute in te r v a ls , c a lib ra te d vol tones o f chamber atmosphere were drawn through pre-weighed glassf ib e r f i l t e r s . F ilt e r s were dried overnight in a descicator co n ta in in g O r ie r ite * before and a fte r sampling. F ilt e r s were weighed both wet and dry on a Cahn 26 Automatic E lectrob ala nce*. Atmospheric concentration o f p a rtic u la te was determined from the weight d if f e r e n t ia l o f d rie d f i l t e r s before and a fte r sampling. P a rtic le size (mass median diam eter) and percent o f re s p ira b le p a r tic u la te were determined w ith a S ie rra Cascade Impactor during each exposure. Impactor f i l t e r s were d rie d and weighed in th e same manner as sample f i l t e r s . Chamber oxygen^tas measuqfid w ith a B io marine Model 225 Oxygen Analyzer. D u rin g g S R S S fS i^ P Q s u re s , chamber temperature was measured w ith a thermometer, and r e la tiv e hum idity was measured w ith a Bendix* Model 556 psychrometer. I I I . RESULTS: A very lig h t m ist was v is ib le in a beam o f lig h t during a l l exposures. Chamber oxygen le ve ls were maintained at j> 20.5%. For 3% TBCU-AS exposures, chamber temperature ranged between 20-23C, and r e la tiv e hum idity ranged from 27-39%. A comparison o f wet and dry f i l t e r weights fo r both m a te ria ls showed n e g lig ib le weight d iffe re n c e s . A ll reported values are based on dry weights. - 3- Company Sanitized. Does no! contain TSC^ nt?( Atmosphere C haracterization and M o rta lity P articulate ^ Concentration (mg/nr) Mean S.D. Range 4.3 1.8 27 4.2 34 1.2 150 35 2.0 - 6.5 21 - 33 33 - 36 110 - 200 X Resplrable b Mass Median Diameter (um)c _e - >99 0.96 98 1.8 98 2.2 M o rta lity d (# deaths/# exposed) Day 0 Day 1 Total 0 0 0/6 0 3 3/6 0 1 1/6 4 2 6/6 a P a rtic u la te concentration represents combine . concentrations. 0 % by weight o f p a rtic le s w ith aerodynamic diam eter less than 10 um. Calculated fo r to ta l atmosphere. Day Q in d ica te s during exposure. Day l in d ic a te s w ith in 1 day post exposure. e P a rtic le size could not be calculated due to in s u ff ic ie n t weight pick-up during the p a r tic le size sample. 2. Observations: During exposures: At 4 .3 , 27 and 34 mg/m , ra ts e x h ib ite d c le a r to red nasal discharge d u rin g exposure, an e ffe c t common to ra ts under r e s tr a in t. Deaths occurred e ith e r during exposure o r w ith in 1 day post exposure. S u rviving ra ts a t a ll concentrations e xhibited s lig h t o r no weight loss 1 day post exposure, follow ed by normal weight g a in . No adverse c lin ic a l signs were seen in any ra ts d u rin g th e post-exposure p e rio d . q .n 4 'Ssm pasy Sar^feed. B ess no! eenfslsi S C A CBS Particulate _ Concentration (mq/nr ) a Mean s.D. Range 33J8e 12 2.7 5.5 - 40 35 - 42 43e 14 26 - 63 5439ee 2.6 2.4 46 - 54 50 - 57 71 11 60 - 86 % Respi rableb >99 96 96 >99 >99 >99 Mass Median Dh i iameter /(1u.Mrn\) 1.1 1*8 1.6 1.1 0.84 0.86 M ortality /(a# Jdea1.t1hs/#a exoosed)1 Day Day Day 0 1 2 Total 0 0 0 0/6 0 0 0 0/6 0 0 0 0/6 0 1 1 2/6 1 3 0 4/6 2 4 0 6/6 a ic u la te concentration represents combined _______; o n c e n t r a tio n s , _ * by weigirt o f p a rtic le s w ith aerodynamic diameter less than 10 urn. Calculated fo r to ta l atmosphere. Day 0 in dica tes during exposure. post exposure, respectively. Day 1 and Day 2 in d ic a te w ith in 1 and 2 days Generated w ith water bubbler in a ir stream. 2. Observations: During o r immediately a fte r exposure, some ra ts at a ll concentrations exhibited red ocular and/or nasal discharges, e ffe c ts common to restrained ra ts . Deaths occurred during ' exposure or 1-2 days post exposure. C lin ic a l signs in ra ts which subsequently died included labored breathing, le th a rg y , hunched posture, decreased muscle tone, p a llo r and/or brown ' nasal discharge. Survivors e xh ib ite d l i t t l e o r no weioht loss and no s ig n ific a n t c lin ic a l signs during the recovery p e rio d . IV. CONCLUSIONS: An inni h ila t io f ^ U k fo s 27 mg/m o f s o lid s . An ALC f o r i s 4Tmg/m'* o f solm is. According to Haskell Laboratory ToxTcTt^ s ific a tio n s , both m aterials are extremely to x ic by in h a la tio n . - 5- Gomoany Sanitized. Dees not contain TSCA CBH Supervision and Report by: Technician W illiam J . Fasano W illiam J . Fasano Technician i Study D ire c to r: Approved by: A J 0 :s g l:2 .2 3 Date Issued: July 25, 1984 u*/. su. J . l Gerald L. Kennedy,/J/*. Section Supervisor Acute In ve stig a tio n s Number o f pages in th is re p o rt: 6 - 6- sfipg Sa n it& e d . > sot ceciato Ti Cf?f