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.r E YB!RESIEA1RCH AR226--1771 r Analytical Report I Fluorochemical Characterization of Filter Samples _ Particle Size Distribution Study 3/04 | Exygen Research Laboratory Report No. L0002178 [ Testing Laboratory = Exygen Research [ 3058 Research Drive State College, PA 16801 F Requester Michael D. Aucoin URS Corporation | Barley Mill Plaza Bldg 27 Room 1260 [5 Lancaster Pike and Rt 141 ( Wilmington, DE 19805 35 gp 39 I" 2 I X= Ban, usa s pace 1075 cn3n0 gyy WooAmnScomH 1 Introduction F| e oLasbuortasto_raireso,-rIna.po(rdibaofdoE-rxygtehne.Raensaealrycshoi)fs(Efxygetn) feomrDrueP_coenitvseCdorbapoyratiCiaonnt.mro_TAphnisallryeipcoaretl s presents resus assigned o Exygen Research study number LODO2178. | Speci fluorochemical characterization by uid chromatograph/y tandem mass spectrometry (LCMSMMS) was requested for all samples. A total of 29 samples were received for analysis. I Thesampleswerepreparedandanalyzedby LC/MSMSfo the following stof uorocherricas I + Table 1:TargetAnalysis r [Com--poungNTAPa oFOoAnmm e I 2 Sample Receipt I wTinehfreoersmaactmoilpollnoecistepwdreerosenenst3ueb2dm5ii0tnt4Aetdaaicnnhdimne3din2vti6dC0u.a4l palansdticwecornetairneecresi,veatd aomnbien27t/t0e4m.perCahtauirne-.ofScaumspiloedsy [ 3 Holding Times --~ TThne ranaalmcealbmotesuseteddwvhaasvoaldcaodtde saogaaaimarnxismrutmhkodigngttimeofd14 daysein lear witin 14 days of colecton. | 4 Methods - Analytical and Preparatory | 41 LC/MSIMS 41.1 Sample Preparation for LC/MS/MS Analysis | PFOA was extracted from he fterbyshaking ovemight wih 40 mL of reagent grade viter. . TuwhabesewafuotsreedsruwtbaosesparruoeepmnaotrveceltdehfoarnsoupammtaphnedesscaofnmocrpelnLetGbrMaytSifIoilnMtroSaftitaohnnaeanyesxdtsrt.arcatn.TshfSeeorirsdendiptvhoeas4ao50 0emxLt.mraLecxtctieronantcrt(iSfvPuaEgs)e | Gtruaanlsofweraresd dtoisacaCrydesdS. PEThcearctraitdigec.geTwhaosctahretrnidoguetweadsietlhut1ed00wi%thmo4t0h%anmle.thaAno5l:mlw.atpeorr.ioTnhoef | methanol was colected for analysis by LOMSMS. This treatment resuted in anaightfod `concentration of the samples prior to analysis. | | } pacezors | GNN578 [ 412 Sample AnalbyyLsOiSsS f anAaHleywze-tPhaecskaamrpdlHePe1x1a0c0tsH.PLACnaslyyssitsemwecsoupeprlf1oe0rdmaeMdicUrsionmgassseUletcitmead MreSacMtiSown aosnuiseodnto__ r an(aSlRyMt)i.caldSaatmaplceasnbweefre eoxtrianucAtetdancohnmde4/n3t0D4. and analyzed between 44/04 and 477/04. Faw 5 Analysis | |- 51 ACa6l-ipboriantciaolnbration curve was analyzed at the beginning of the analycal sequence and 5h0v,ou1g0h,ou2t5t0h,e5u00n,foarntdhe1c0o0m0phoou.ndGsyf. 3esrpesa.fTmhoencsalabnrcatsonapiai(nsFweOr,eprTeoptarseedant2o5r, [_ `(rwFee)iswgphetorinensgvd,ettrehmsenusislnotephdee., cyAo-niccnetneatrrcealpttioabnncwudrracvsoepraliroestlatatcetdicoeinfpotrcooaeebfalfnceichfiep0noti.n(t9r.) 9aUn2sdi(n*cgoe0ifn.fci9ac8ire5nr)tegorfedsestieornmwiniahti1on I fCoarfstarmaptloens.standards for MS/MSanalysiswereprepausrinegthde same SPE procedure used For the resus reported hero, callration criteria wor me. | - 52 ExeBtxrltaraaccnttikioosnnbbllaannkkssswheoruedpnrootphaarveedaannydtaarngaeltyzaendawlyitehsepverreyseexntartacotriaobnobvaettchheofcosnacmenptlreast.ioTnhoof he low-evol calbraton standard. For these samples, ha extraction banks were compiant | hlIeanvvsetelrcuaamnlebynrtattbairloganentksstaaninnadltayhrtede,sfoaprnrmdoesfaefncttlreaaktnnoomrwetnahbahoinvgohel-ltsehovelevlelsnoatwmAwpaleveresol.aclasAlogbaraiannta,lotynhzeesdtbaaanndfatkrrsde.svheroFyuorlhitnghohet- samples prosented heretheinstrument baarnecomkpliasnt. | 53 `SSuurrrrogoagteastpieksesarenotacompoofthneLeGMSnIMtS analyical metho. | 5.4 spMMaaktterirdxixsaptiSk2pelseivwkeelers/esannlodatabnporarelapyatzroeerddyffoCorrotnPhFteOrsAoe.lsAaSmcapclomeppsi;laehbolswoervero, tcwo bowlaenrkvefseeertaprtap7a0ir-s1w3se0r%e | Sampo recoveries wers win the acceptable range. Matix spike recoveries are given i : Attachment B. 55 SDaumplpicdauptleiscateswerenot perforfomrhedsstudy. Pacesoes n0579 I 56 `STawomapnlaleytoRaellrauntsewdeCroopmemrfeonrtmsefdo ualtycontrolnarrativefo each un. compthleseamtplee analysis. The foloiwsaibnrigef f "AnalRuy n Numt ber i Sampc lesAa nalyl zed Comments |- TOGA WWWWKKAAEEITSSTI3,, WWWWKKAAEETISSI1Z4,, rAToseampaldedsitooxncaolntdWiuWtoKn.AFABl LoeKrT | WWWWKKCAAEEL!--SS11658,,WWWICWAKEA1FSBTL.K61A,, datas useable, WWKAELS21, WWKAE!-S22] |- WWWWIKCEAIES1.2S32,5|, WWWWKKAEA1E-!52S6244,, WWWWKKAAEE!1 S53226,5,WWWWKKAAE1E-1S3S-913,, I WWWWIICCAAEE11SS3346,A WWWKWAKEATE-LS-3S5,3 68, WWICAE1S51, WWKAET- I~ S$5542,, WWWHKKAAEE11-S85553,, WWWWKKAAEEIT-S560WWKAE1.S5:68 SMIAEIS13 agiSrd - OHO04AR WWWKWAKEALESITSST,, WWWWKKAAEET!SIS-I2,, Aldatais usoabio - WWWWKKAAEELS2S126,5, WWKWAWEK1E-TSS22-31,, f WWWWKKAAEE!-SS2264A,, WWWKW-KAAEE-LSS225., I $885,2,WWWHKKAAEE11-SS333,, WWHWKKAAEE!!-- |- ES$31G-54,5A1W,WWKWW-AWK-KAEAE1-TE8SL3-3S565,82W,,WKWW-WWAI-KCEAA1--E1553, WWKAELS5.4, WWKA- 5 AEE115555568WWKA1-S5.6A, WWK- _-- | 6 Data Summary | 25Plauags/afslcreats PaFOcA hAfmoraedentated ising ofthe analytical esuls. Al resus are reported | _ Pace ors cnnsse | |- 7 Data/Sample Retention [7 lfScoaltmdoepnrlsiemso-aadoiaasnpetobaasyireExneoyfgedoondno.enmeoreihvaefeer tmhoedrae.paorntdshirsssuecdauyn.lesaspoothewrweiscerspeecdifiiend.SoAlnl I 8 Attachments [ 81 AtactAmRoesnutts 82 AttBa:Mac trih xSpim keRe ecovn erit es: I~ 83 AtachmentC: Chan of Custody 84 Atachment: LOMSMS RawAnalytoal Data [ 85 tachment: Sandrcs Data ( 9 Signatures | | MF 7fd Rnery, Operations Manager I Kevin J Lioyd, Vice President || Other Lab MembersContributto Diantga Karen Smit vbr Dato "Yo Date pacesors CNNG81 [- E n | xX) "og g oeecRn. LPT E [------------" I oupontsampetcencaton ron Gorter wWWWHhKKAAAEEE1LLSSSTT11S2 aasazot [" WWwWWwKiKAaAEsELrLSsSTtTsAOA asimis Fo WWa WCICAAEEm L!SST.2618 2aW1s [= WWwWwKKkAaAEeELr!SsS2a24o2 0w783s0 fo WWWuWWWKnKK-AAAh-EEE1!eT-SS8s2O2-6Te53 312T.348e90 [ . WwwWwwKkkAaaEeg!rrssSss3s24 ossi6as 2s | . WWWWWHKKKAAAEEE1!L5SS3IS5ES3A 2 2o4s0 I[ wWwWwwKkkAaaEeeLrrSssSss12s TT1ae2ts8 WCAELS54 bE [i7 - wWWWwAKdK-aAAEeE1iT-sS85s5-e65n8 ic1.ee37 Limi Doteton (LOD) forth procedurs s apposimtsy 0.0004 utr _ Limi Quanttaion (LOG) orthe procedure fs 0002 upfior NO. Compound not detected ND <L0D <NO<10Q = NQ - Compound detected ata level between the LOD and LOQ. Result is not quantifiable. LUU6 pronen Rice EmgeeanPson, usa SLITS - Attachment B: ! Sample I: [seme LC/MS/MS Laboratory Spike Recovery | . | Por | o comSeoaan tin ]CTGone| eonw I 0 1 as 1 soo [1 encom ----------1 po nen Cancntatons present mtprsentin ho alo oes | emcerra | | pass | I. | | i -uu7 600583 . Attachment B: LC/MS/MSLaboratory Spike Recovery Sample 10; {7 spikedAmount (ro) -- -- - I~ I Por [= [==lon] Gonconaton Rost Fost citeria |_____ cou1 0 1 Coui 7| eam isa f_(Pass/Fah) | J PASS | LowsRecoveLriyt [emeri Concentrations present amour rose inthesample exact ~ | - { "uus I~ 00584 i- Attachment B: LC/MS/MS Laboratory Spike Recovery | Sampl0e: [mem mee -- [ Wis Saen a-nS S |PoesaSt |orarie [ reononions [po ne `Concentrations represent amount present in the sample extract - I I. | - C09 cr0585 : " Attachment B: LC/MS/MS Laboratory Spike Recovery I-- I ee ! =en Teoh a RE |r | won | en | por 1 0 [so | ves |emcsriee|n | Pass | IU armors [une Conentaions ops arnt presenotvsst ! -| | 070 - cnorse ! [E [ hn S3t0a5t8e RCoelsleeagrec,hPDAri1v6e801 RESEARCH PhoFnaex:: 881144--223712--11508309 | o R Praecte Poacor2s S CL GrGonoormm CSOCa: me TTuewas | CSoonmmpiatneydNBay:me: DMuipcohanetldbe. NAeucmoonur&s Co, ne. Coniorm Request. True I_ SLougnneTsyper 7Immediate Recaptof Samples | DDaiteesse: 0c4u2e1r2z000s4 |~ RReeccoavneeddBDya:te; 00A1m2M7E12R0M0A4N, MARK LSpareoad Sample: | PELrroorjaoeoctnNoTStiOtelIseR/.Type: ARnIalSysAisKoAfRPEFNOA in Filter Paper (Particle Size Distribution Study)/ROUTINE [SSmEAiIRtNeGSr, reesei ete t-------------- [- rpaa=ge coon sferan svi Packages ever S/ CpopnrtiaiDners Package ome hasmnisod | stssesisasneso Tomp.Cponovolfemp. EH DAissEiSoRnE/AHaDnBdedaBy | CCConotimaieonse#r Gri1os8os3Ws0eight pH CSSoonntoiadiencaerrppTaayssptteee ProsNTooornanteeive Mig. Lot [- RCComuEesmz Top 15s SSSooommrcaocnu pppaaasssttteee NNNooonneee CCCioaomooassts 1T13e10y55 SSSooommrcseecuns pppaasssttsee NNNooonnneee GGiomssoossr 11603250 SSoommddawrppatsee NNoonvee | C0GG0no3s0os0o3ss8o 111865300950 - Coon 1015 5SS0oommiccdoaemrppplaassstticec NNONooNrrEee omc psc None [7 GCooomnose Gnooss m10s2s0 1608 SSoomrcion ppaasssee Som dosplace NNoovnee None GGoownss Gusoowr r16e2a0 1035 SSSooomtmcoeaem ppsaaststteee NNNooonneee Cioos ise ( cGonsionoss 11380705 Som cet pas None S50ommi ccear pplasstic NNoOnNeE | fG Gorsoeoo set ma1025 SSSoomomdmeccoeats ppraststcee NNNooovrveee GCoiosse 11003199 SSoomm cdeausr ppaassee Nnooree |{~ coos0se. 189g 50miclear plastic NONE S011 !I" wLnoognoo0nn Report VarsionP:adMear 1o1123004 416M nnooss5Iyns5ta7nce: Mig 1D Rovssorr [NEN . [coLomcopz1e78n001 | Looozrrsoone ii Looarmsome r Looo78.005 Lonoz1700006 [canon [7 womrrnoon Lozrrocons | - Loo2recot0 Looozi7s001t -- [" womarrsans | 00021780014 torrets | Lozno0re Lonzrrecorr -- | wocermocors Lorn | ! 3Lo2g9i0n0t4 comme coonnas covmnzs consaaso coos covnz coven coosooss Coons connonse `coaso0s7 coon coonnan cononio co0s008 conmonz cononss cononse conm0ss coos comer Samples SwOmD FilrerPmaper WsKamApE1SHt OsctaSsamaps toevaRmeasosovsed Duontavm0e SOLD Filer Paper WWKAELS12 ouzs2004 03272004 OarTZIZ004 SOD FuerPaper WKAE1S14 RT ---- SUD Fierpaer WKAELSLEA wo rare mss SOUD FitePaper WWKAFBLKY SUD erp WhKAEHS2A SUD Ferpaer whkAETSE2 ET -------- op rer cass soo rarer wiaErszs NL...a. oazosos camoos owns aazoaoos cammaoos owns casaoos oaamaoos uname ns carn oun consao0s oars outa cameos ovaraoos ura ozoaos cammos oun aaznaoon cammoos ounaonn cman osamions ours cnn ovo ours SOLD Filer Paper WWKAE1-S26A 0u282004 0322004 041122004 SoD erp WhAETS260 cozeao0s 0370s ourezone OUD FacPaper WKAETSEA camsaoos ovamaoos uname SUD Ferrer WKAELSIZ S35 rane wnrann camsaoos ovaraoos ounaons A --- SoD erp WASHES vamos cares oun OUD Ftscpaper WWKAETSHS avzszoos cazroos ours Report VersioPn:aMgaor201132004 4:16PM2012 instance: crosses Roos3077 (INNER ame con JP [eons cons wp rar asios [wm carn PE -- mma E woo L0002478.0024 SOLID Fiter P--apWeWr KAES5-2- ree asians | - -- 0030052 J ss roves anions I-- ca A [J cas OUD irra WicAE1S564 mma cn wo man asin A, mn orn cua mn on oo -- 034262004 02142004 04112/2004 rns oo ou renee ove eros owns ovzsaoos ovaraom ounzaonk Slane |sn rrremgTrmatsgotB010123 e-e | tone ' rosso IMME [g 21 [ay 2B IE > S ER tog EZ o REEREREER FE IR | =z | Tn REEEESERIRE> a PEELE IE C ELBLECLEE |Z :: EFS4 IFHN]A SCE : HS EEE "La EF EER] ae OC k Iz 9 |: BE IE z || Efe iH [ELI TFLREH CAD E8kT BEefL"EEil c== = I8 PRES = 3 c BET ho 27 sepanei | 1 | ERG, Te| BIR [ XP pe peon 1 : 8 oz281 33) Sx1B0:; ! 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A SAMPLE CONDITION UPON ro RECEIPT FORM Protocol #. Soon stay Date&TimeRecoved _ Condon ofSamples A fl otfiplespe nla t `Temporary Storage Location Decca? taissoate __ hghaly waits f443 hae gysg A tearting oortedy noche. Migfopr 1 i | i uoig casa y ~ yr vi JALAN lisa or lle 7 . | RllalH ge + (r |== BF= bFTe eoohrf PeEEToEsRgt C LB= E L LE EEeEfoso CC o phboEg A aW] yFelt eeRi CTE E th {iai] a i = HE He | |= le ea EET LT TE 019 eronan .'- spent StyNo 2FoEs na ey om LinkofQuasi m Hoe oB Seven: 5054 erper) om. sSaneon EE ower E m t Efr i" a See ow BE EE v mira a an oy i moi Tote wim eww ow - ErEmaSA IOB E iS aZ mi PoeEm EwomoNow omon f Ter mam EEE CIB og oe ) BEC fERL HE a T J--E ----m-- resE-- teesrion 0 a dE . bmw CaEt. LLEsE,R. EE ESRoE oammisn. | SEEERT rm Hilo 0020 . Cr2596 i !; E JI~ cosmo Pec s1427210% | or Cw State College, PA 16301 Fax: 814-231-1580 | reel. wd | Exygen StudyNumber: Internal 12178 Chainof Custody/Fortification SMhaereit: Fite Paper . Teested belowvereremovedfomromStuex. 04 sors cabinet nis _CEE !l [Ca CLIENTSAT MPLET | SEoXwVGrEiNeIrDNeUcMBmEwRd | |VOLsUMoE(nl)|[ FORTIFIC1 ATIONGD] [se | Whos siFlebspecomol | 00 | | ! [| Soudrinappasga | woo | 30 | y [wm |Vhmmsim|ia e0P| pas2p0is| [Cm |Sowdf| ba wor | ge0s 0 c| [wm |Wwowaim |iweor | pe2s00po| ! [Cwweagisin| wost | wo |] [wwearisia | wma | wo |] . [Cwwwkwaapsisiisse| | wboomsss T [w wo o[[ oo | - [Cwweagisis| wes | wo| [Cowkcakisioa| woes [wo[oT] i [wwkabisies | war | wo [wwkarsua | es | wo[7 [Cwwkapeset| toss Two TT - ' - [Cwwcamse | [Cwwkapsos | tao en wo || wo| 1 [wwearises | wasn | wo |] be [Cwwearises | men | wo[7] [wwkapisoon | wisn | wo [7] . [Cwwwiecanrsisees | | otammsaes 1 wwoo |]| [Cwwkamsiz | [Cwwkarsis | women womens wo |] | wo | | ! [SoC iingsolutontsea | VolumeT Used for piking | Ipisavmate | - [Slot Fit PaperSpA_|__ os0s06s t0ngiml) |3000@00plmicopipen |FA rou [en Fr ip spb] ois stongats | out onalmoog | Bh rodlsafe ' [StottFite Paper spkC_| 0308044(00aginl) | 300,120 pt.micropipen | VA Told [Fmi pespt potions ingen) |ss001 0stmong [IE ull rr Tv. ! 1 rr - rT 1111 '- 4T0immLe_o1fw3a3t0e.r wasaddedtoalDluseampl_eos:d[02)oq iis _LEE. ' An`aCloymsmetnstSsu:mmEanrtiyr:esamplewDaDusautssaesdeetft:oHrextreaction m nitalsn Date Tar, ` Daas -- InitalsDue: O=/4_ . `Sctcxtctonamlyssdataverifiedbg y: E owe fRobrr2, 200400 . Jo 2 n2597 { [[RTERR el CoRTT CoCi oa RP ITE LrE R s RL CC:F PBOiOOeHOIe ITeITeTSgs 4 a LTTE 1) Co JHE ogy gE id fT of \ : f HHH 1: it i NE HH i ii . Bi) si | i CEPFIT ED e E a a ee L022 n0598 . I E OCgYoICo owemeo nnitheteionpnessa142p721e029 ! `Sponsor Study/Protocol No: NA : IN mnsR mrspipis Time __J3IS Date_o4]pz[0 Initials_LEE. Hr 3 T TT T ABCL OT ' WWK-AEL-S34 1217819 wo [1 -- WWK-AEI-S35 1217820 [wo [1] WWRAELSIA | pasa | ae | i WWKAELSISB | tas | wee| WWKAELSSL | pasa | wee | WWKAEISS2 | tamed | ae | WWKAEISSY | paimeas [a0 . 12178:26 wo | 1 | La78.27 | wo| 1 WWK-A-ELSS-6A 1217828 wo 1 WWK-A-ELSS-6B 12178:29 [| wo 1 | rr or Tr rr rr r r I~ 1 Tr rr ( 1 rr = TT [ rr rT rT T - -- rT T | ---- rTrT -- rTTT I ---------- TTT" [CC 1 oikingSolutionUsed | VolumeUsed forSpiking|_taitavbue| ------ rT = TT 1 [ ---- oT rT ---- rT I rT 1 1 T--1 rr 11 I r g-- rm-- at-- esnn--ee Tr finger 1 oy T----1 cri: Sima _ AnalysisSummary: DrDaetanseslte. 003S0001 anilneDeaute: LhgLLolode)ly `Setextraction/analysis dataverified by: Date: tphy , || "0 2: 12599 E TiAegE L--R WFR i aiNa CBSEiHfI:Eai| mn53 a 5 :2 HITE iE] 2kf 2 HH gE | fl --. RENAN JuHdHiH Rutt j ij EE3 HTH .E C HEHTihmoinn | ih alli}ai: i | | 024 crosoo ] FETE|; | g9 52L32x g 3| T w g |8 gs i pills 25 | EjHE 3: 2 :LE2 ed ll e8 = FREHEE2 HE[HEzE8E:E|E3E|E3E|33EE2E2HR3EE2 EI : 32 CE5g3z gEa af [de 3:2 833 3 = is2 - 2 fge EEEERE FE 31 g = = of 2 |COs: aiT i La [ edao aslae lyl | | vEiI vdae esa s o1o1 !- :HE2 3 |2EEBEE fayeai nd B1g k 2 . A8U5 Ha5EyYyE iHHiity o iis ` 3 HHaEaHgEEyEy AEEy H15i55i83 a1| 2 1 Lo i 127 ESi|a-Asll32YzE/8LlE8l8Eg5l(8Ss iH i:ei tis iiff L :2 ' =S H 3 jH eiis I4TsT , | ves Pe mm 3 5 183 __& sssazssssisssssssssassssissssissssssasasazas ' 3c ! | SC rrrtitTINRIEeIRLInIRtIIttITIItRITIIIIITIITE : | q S| 2En |= 2 - : 2228838 issB8ri isi Ei fin : 5 .- g 1i ,53i 8 meBaznesetssfsoesasesBeanEsaEsafseEsEs . | Foc .=, i a3 Ee THLE 553 Cob ed i ! : SERa EIz RE:OEO'E. EE BE ' . 1( ] | &jmoiemHRaRnRnie n . sronsn leen seona sivnns :~ =iz Fn : 22 | EE EERE ColsiE=] f, H SigaiggEnagssazissacsasNnIaaNansanaanntanIL if: Hien g Him 383 - i is 3 rNmTeoroYrNRIRenER2RSNR IRRRERBSYBILBE BBE . |& i rNPTeerea RENT RerR2RNNRIRERRRBSNRIRASERASTYLY . $024 cnesoR is13 | IT | Lo |g IgS) [||Bg 8 L| ii Lg32 i fn -= I = | EE EE J 2 i 2 RRR RRR RRR RRR RRR RRRRRRRRRRRRRRRRY f gpsazEazzrEzzzeeazirestriisiiesiiiisieesiiss po (FSE BEIBMIIHIIINE |g 32 2 t 3u rNmenerea ENR IReE22RINNILERRRRSNR3LE58295Y9T . u027 nosos [ [|g Es Ld] | | testes 208888 5 oereeen f... 1] on Eon | 8 111188 [3$ | | | g2 | F:E 222288 fi inn [ i { z7 gi ol ju L(1i 15 22 4 3333%% L | ae Toon Hf NH { i i 338358 i 2 2 38850n~ ` ii 285238 . I Suzy choco  |LO[RvSa| --| ,-- i | [3 Lo] 3g |8 LE l |g LE { | 2 iv EZ ! |g Cog = 2 8 2 ooeeee CHE{| ' 1858 Ta : seuss $029 C2505 r 1 |: LC/MS/MS SYSTEM AND OPERATING CONDITIONS | `SponsorProtocolNo:NA Exveen Study No: 12178 . Instrument: Micromass Quatio Ultima (LC/MS/MS Unit #7) | Computer COMPAQProfessionalWorkstation AP200 |e Software: MMiiccrroosmoafstsWLiinmditoewd:s NMTa:ssVeLrysnixon3.44BBuuiilldd 1030814: Service Pack 5 | HPLC Equipment: HeHwHlPePtBtAiuPnoacsPkauammrpdple(HrP) Series 1100HHPP CVoalcuumunmODveegnasser | HPLC Column: Genesis C-8, Som x 2.1mm id. x 4 (Exygen ID: MAI3296) (JONESCHROMATOGRAPHY:Part No. FK5962EJ) Mobile Phase (A): 2 mM Ammonium Acetate in Type I Water | Mobile Phase (B): Methanol 6)ot oX |- Analyst: KE3a0vr3re8naReRsiosehaaarchtDrive, State College, PA 16501 Phone: (814) 272-1039 FAX: (14) 231-1580 L NOTE: The next 3 pages are computer generated printouts from the masslynx software program. The pages contain the | instrument settings used for the analysis of this data set. | All Handwritten Peak ID's by: 2 los et - 1 - I 1 i i | W030 cnocoe | scanning Method Repoxt Page 1 | NE achos:R W G\MASSS LIN\GI 08APDS .BRONACRUDBNG08 EEO Printed: sun Ape 04 07:18:19 2008 bho glo |J -------- 0.00 |; FSeouonrnctiCcoornaatm:tmoe1ndseyttaMyRMofsec1s 1+ Mass Pa50.i:0r03 ( ESP- ) | ERSeapEneaRtesi:ne wThTis 5:5:0000 : Channel Parent Daughter Dwell (secs) Coll Energy (ev) Cone (V) | 1 413.00 369.00 0.20 10 10 | | S031: | rasa? | onesa rrose a mew rms Yl i p2 e oo | HP1100 LC Pump Initial Conditions vo } -- | rOvieen nTem1pcermatpuraaRriighatn(<CT) sar 3500 | The -- gradient Timetable contains 10 entries owhich are : 0 re oe RE. | The Timetable contains 4 entries which are : | son nosnepto mist comtsions | [ERE Si ie - Draw Speed 200.0 | LUs2 graces Ey -- - Method: | einen: C:\MASSLYNX\008 APFO.PRO\ACQUDB\PFOA TUNE Sun aps 04 07:16:36 2004 d silo Page 1 | Dau 413.00 somes ( psp) Lo Cocnoe.min | m fRobnnaappd oollee 21 SDoeusrobievatBiloonck TTeemmpp | | pressures | a=nale yser Vacuum Set wo R2a9k 10% To x000 100 300 300 Ra o$e5r Analmyesedr iIEnReersay1 1 CoSprtyirtaanigoen Ih Res 2 HBTaMElnteRierspelyi2e2r Gas Flows SecsoneelvGaatsion Sweto mak 1i0 52 00sh. leo 231061%0.0 ess Rac 5i8t.2t | Joss 10309 | \, gen ProPcreovReensRaerucsh. | In order to verify mass resolution, a calibration check is performed on the mass spectrometer using a Nal/Rb/Cs tuning solution. The tuning solution is infused | oMnStostchaenmfarsosms8p0ectous1i3n8g0aanmiunfiusspieonrfpourmmepdsoetntbooathflMoSw1raatendofMS120.uLTmhien. An c=al0.i2 braationo:mfisthcueoinrsikdneroewdnamcacespsteasbloenifbotthhe MfoSl]loawnindgM1S12p:ea8k4s.9a,re13f2o.u9n,d1w7i2t.h8,in | m3a2s2s7c,s4a7r2e.6o,ut6s2i2d.e5,th7e72+.40,.292a2m.u3,, t1h0e7n2.t2h,e 1i2n2s2t.r1umaenntd w1i3l7l2.b0e. Irefcaalnibyoraftetdhaend another calibration check performed. `The printouts from the calibration check can be found on the following 2 pages. L pS | Xp Rionva | 0034 crocs O6R00E28E13G21L0 || s-3m8% . i: AH i8 T ou hi lz CA | <Q [ %= sl % 5 FE 2 $8 ii vB 5 288 ial aE| : 5EE . |: Yged . ge2 8 l Sg5 EE 58 2 Cisyy Lo CB gE 0-0 | a ree ,8 ig i. -- -- Be 8 | | B3 : # 2 | 0035 cnosaa Fin Leg- get | 59352" || | 33 oo: IE | '|= | E$>Iyg8ka 71 8-48 2 9 | :| Ci: b'ry: |= | | 5} 28 : El | 2: i | 5s |J:l<8 | fi CFSE | oo E i : | g| F := so F g = =) D 0 : _----3s eesE ee 4 i | : . 0 036 : nestat: ~ tomw a aam | en -- [ f e eer treremS r ol Thefirst curveof hedatasetis used todetermineperformance. A linea regression (ith00weighting)isperformedusingthefirst - N m bpargaele r cs`SEtmaEndAasrd Sre : r e Soa RE S ES nest: So " : wee 8% L soaroomr Wo) f-- is EE Yr a Un [I : w=g ar r E e w ee SE = Paes | E e=e I E EEE EE EETTETT | + 0037 grossa I CquayntiCtyEacail7iebr:ati"Soenk NRoerpoTrot0301A, Ext.Date:04/03/04, Analysti.nisha rage 3 ["CaEliL Fbmrratamioni: P:\DRarta\LCHSHS?\HNasEslyHmx\008 h APO.PRO\CArveDB\O4030T4ATFilter -- Paper | (GcooetmhpcoonwtodftDmetaermmienartrioon:n0.-- 990013 -- [| = (R(CCaulroiibormattyipmoen: ciLuirnvpeea:rt,15Om1r.i0ig7:1sE*xxcfl+ued1e0,12W.e0i8ghting: 1x, Axs trans: None i | 2. 1 a 1 | |co i| aN a| otW(EERHEDEH,LAASLHDAEPILDEBF.ARWAHIIEES.NSHEWSTTVAENRD ||| | Wat NS.QRR{NER 1S oBYIOYS | " . | | VR GETROUT EP TEST, Holsbt * || Ir | | [reo 1 || | I * | b | % i | I | | x: % | | t | - 09 PT3%r 0 T i%0 soho er so T todo5 ! Bryson Raseasch, 3058 Research Drive, State College. BA 16801 (3g . r06aa | | |. FEEx-- ygenstudy no: 12175 [em Huge rorTestfo Outliers' AnEalvyisiasnDaDt:s:0040440/30044 Mam We - Approach: Omitingth suspectpia,thedaasrploredand "best iting" ln | fdoertehrimsidnaetd),(MTahsesavyerwaagse urseseiddtuoaclsalfcoulmattheeafliineedalr cnuravreeacnadlceuqluaatteidonand comparedwiththeresidualfothe suspect pint according tothe following | equation: M = [sus-apveeracget] / average IEM>4., the points considered an outlier sndmaybe rected . -- 3 | 2. Thesuspectpointi indictedbelowinbold fon. B 35 manosasms WSxS odreispideualsms enosts h 50 n meso omn omne BReo - 3S000 TTOeBs0s 6s0l9s 2A60885800 2306382558500 | 130500 1a5n2m0s8s3s0s80 1m40e882 -s11a20s1.s080 16122s0s1s0s80 |~ 00 1E6e01e8 IiRsIlO 1S60I2S9I8020 1s669012s935020 [S000 aTTeOs0s 2s0ess I7TI0O4L0I0 17703314021700 | 1000 152083080 160139 8055920 5055920 avenge: 4334972 | MMaasoo:: 337839 TO ---- } Coenof dd1) | -- poe (son x@) + 10208 | ; _. u039 i nastS | QSutaundtyiNfoy.:SLa2m1p7l8e, ReSeptortNo.:040304A, Ext.Date:04/03/04, Analyst:X.Risha Page 1 Sample List: P:\Data\LCMSHS?\Masalynx\008 APFO.PRONSampleD\O40304A Filter paper | _ HLLeaatssnttodmmsooddiiffiieedd:: TMFiuo\enDaMAtaparr\L20C35HS01H60S::?4584\::N23a42ssl22y00n00x44\008 APFO.PRO\MOtEDB\031704 PFO & | ----dob--Coder-- Printed: Mon Apr 05 07:35:20 2004 Beeoek.TBOIETRoUT5VTo S--UES--A--=--05D | Neaxmees: 040304A-1001 Ll eh op bo : | Re XC032304-0, 0 ng/L standard | 040304A-1001 Sm (Mn, 212) 1001 ] ~ | | Tm ge nae ies awpa 3% y AOR 04-Apr-2004 08:19: MRM ofL1CCIhManSnIeMlSES- 413> 369) 1.1684) Areal \., mar m rrawTime | Exygen Research, 3058 Research Drive, State College, 7A 16301 0040 2316 | QSiuaaanytiofy.:SLa3m3p7l8e, ReSpeeortNo.:040304A, Ext.Date:04/03/04, Analyst:k.Risha Page 2 | NESeaatmhepomldeesbLiiesti:ie vBeP\inb\aDCaat\eC\oCLeHCSHaSNtHSvS?e)\n\H:Maaagssss1slyiymnnxsc\\000088 APFO.PRONSSmpLeDB\O40304A Filter APFO.PRO\NOtDB\031704 PFOA paper | daesbtcomnoedified:B Tus MB ex 35 105413A 5 200. 4 prert 1: pron SE | [XC032304-1, 25 ng/L standard 04-Apr-2004 08:36:21 LC/MS/MS [lw4)i | x|040304A-1002 Sm (Mn, 2x2) 5.33655.6 MRM of 1 Cha4n1ne34l>23E30S64-9))| `area || [LIo N To zr on ab ae b sk sebm sEsihEeEn08Time 1 | | Exygen Research, 2038 Resssrch Drive, State Collage, PA 16801 | 0041 hone? | oB mite sume eTmSe.rycobtt04n, fu.Datas04/03/00, Amslyetor.nishe rose 3 Sample List: F:\DACa\UHINS\asrlyna\008 ARFO.PRO\IAELeDI\OAES0IA Filter paper [ | Le ast moe dEifieed:e MBon EApr R0r5o0Eu6:4r8Ta:2o s4ri2r0o0u4\108 ASFD.FO\KSCHiIiNiGIETON PFOA Printads Men apr 05 07435110 2004 pes [pm Co 1XC032304-2, 50 ng/L standard - | 0140003,04A-1003 Sm (Mn, 2x2) 11 | i i %7 | oder reese Too zoo soo | abo 53 7233 04-Apr-2004 08:53:02 LC/MS/MS#7 MRMof 1 Cha4n1n3el>E3S6-9 8.11e4| stoB6E 00 e7 me0r0 yboTime : 1- | i Horan nssenssin SH Asoaseh Sriven FAA SHIRE Th 44% 04 CNAs quantiEy Seamp.le ReiptortNo. :040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 4 | NoSEatmhpomldeseListl: B Pr\Deartao \obLseCMeoSncHsSin 7n\HaHaatsssse l1i yynnce\\000088 APFO.PRO\SampleDS\G40304A Tilter APFO.PRO\NSCEDB\031704 PFO paper Feoret hsnaeied: TuuoevNaars 3233 10:54:32SA2004 _ _ | Mame: 040304A-1004 | . [XC032304-3, 100 ng/L standard | =|040304A-1004,Sm (Mn, 2x2) [| | ! 100 200 300 400 re 04-Apr-2L0C0/4M0S9/:M09S: #7 | rsaars MRM of 1 Cha4nnet1l>e3Es3S0-)9 Areal errr Time 5.00 600 7.00 800 | | 1 = xygen Research, 3058 Research Drive, State College, PA 16801 1 . wlU4s 12519 Qeuayntifiy. STa1m2p07l0e,ReSpeotrtNo.:040304A, Ext.Date:04/03/04, Analyst:X.Risha rage 5 Sample List: Pr\Data\LCHSNS?\Masslynx\008 APFO.PRO\SmpleDB\040304A Filter paper | HLlaaatsshttodmm:ooddiiffiieedd:: MTouAneDaANEpaarr\L02C53NS01M60S::745\84H::2a34s2s12200y00n48x\008 APFO.PRO\NGEEDB\031704 PFOA f---------- | Taemxet:: oso20an-2005 |> 1X:c0p3r2on3044, 250 ngSElL standard | (eo |040304A-1005 Sm (Mn, 2x2) | 1007 or es, 04-Apr-2004 09:26:25 LCIMSIMS #7 MRM of 1 Channel ES- 36004 3.`7r6e0a5 ol[o] | OTT ie ato soo abo soo | 600 700 800 | " Exygen Research, 3058 Research Drive, State College, PA 16801 | L044 C2320 | Quantify Sample Report Page 6 | ete tives LLaasgtkSmooddiirfiinead:: Ma3on\n tAppnro00850EaN06S6:?41e8:a28a42E\10L200y015,4A010101sAeBrFoD..PrRrOo\vSaRsEtBoAmAD0I3\1O7U0E8SOA9A70FMAeeE paper | gmeremresar smtsoem wnoex 03sonesmmesjeozsoeneiiel || een [XC032304-5, 500 ng/L standard 443044-1000 Sm (i, 22) 1| | 1% 1.00 2.00 3.00 573515 400 |500 04-Apr-2004 09:43:08 LC/MS/MS #7 uo Chpaele=5k.7.53e5) _ 600 7.00 oe 8.00 | | ! 4045 { nn2AaR: QuantiE ty sae mple ReepeoertNo. 1040304a, Ext.Date:04/03/04, Analyst:K.Risha Page 7 | NSDeatmhpaolde:aListls sHPRer\oeDaaErtea\\LoLGsGHNSpENNSeST?e\\HNdaasssslaliyynan:xx\\00008S APFO.PRO\SampleDB\O40304A Filter APFO.TRO\NEhDB\031704 PFO paper E Mat aateied: Tee Mar 33 1o0:54r3 2008 [---- Men Ape 05 07:35:20 2004 | NTamee: 040304A-1007 Te | [XC032304-6, 1000 ng/L standard 040304A-1007 Sm (Mn, 262) | x4 10( |" 04-Apr-2004 09:59:4 MRMofL1CC/hManSn/eMlSES#-1 1450.38782 4131.>5A13r6e66a9]l | || - Time 1.00 200 300 400 500 600 700 800 | Sxygen Reseaxch, 3059 Ressarch Drive. State College, 7A 16601 l u046 naan | gBEumEseRmerfo. 04030iA, Bet.Date:04/03/04, Analyst:E.Risha raze 8 | cE utmphlee n Lists R D B:\Datu a\E LGHSn HS?\aE us atssslLyyma? es\\000088 APFO.PRO\SsEpIeDE\O40304A Filter APFO.PRO\KSCHIDNG31704 FFOR paper [eednecetoas TuorWeant255aS01n s41 2X004 r---------------- an [0| a: PPA 23 XC032304-7, 250 ng/L check standard 04030441008 Sm (in, 2x2) sa | 34299 |e L v Too . 200 . 800 |400| 500 | 04-Apr-2004 10:16:31 MRM ofL1CC/hMasnSn/eeMlSEoS#-7 3.4a565] 7me 600 700 800 | | | Bryson Resessch, 3050 Resaarch Drive, State College, PA 16803 | V04dnan0a Iayr os. 2207e 0, "Gt No.:1040304n, Ext.Date:04/03/04, AnalystiK.Risha Page s sample List: P:\Data\LCNSHST\Nasslynx\008 APFO.PRO\SempleDB\040304A Filter paper |a LMTeaetsshttodmm:ooddiiffiieedd:: MATouDmsatANapa\rrLC03H53SN01S60T::\458M8::a23s42s122y00n00x44\008 APFO.PRO\HEEHDB\O3A704 PFOA | parmeoyr oso30an-2008 | Cowen [Methanol Wash . 04-Apr-2L0o0m4MJSIoMSvy | 01400030,40A1: -11009 Sm (Mn, 2x2) 71550 643670, ma . of 1 Ch4a1ne35l>23E1S66.93 so Jibei? | ~ *}7 | be 3 | ogo 9% a0 U4714 52=a "IG, Sl75704 |~ O Nen Aftr Tr ar a h nm p inaa Amsn hSAr VE eke e TheafotTime . . \ ~ Exygen Research, 3058 Research Drive, State College, PA 16801 t u048 C2321 QSuiannityitNoy. S1a1m33p7l8e, ReSpekorNto.:040304A, Ext.Date:04/03/04, Analyst:k.Risha Page 10 | ZSaasmtplemodLiifsti:ed: MPo:n\DAaptra\0L5CM0S6H:S4?8\:M2a4ss2l0y0n4x\008 APFO.PRO\SampleDB\040304A Filter paper i |g Mleatshtodm:odciditfiheods: TP:u\eDMaetra\L2C3MS1H0S1?5\4M1a3s2sl2y0n0x4\008 APFO.PRO\MethDB\031704 SE _-- PFOA ~ i | mTeextrs osor0aneo0 veeen | . [Slotted Filter Paper Control [Fe040304A-1010 Sm (Mn, 2x2) 1 | |* | O HTe T TRre assy hake 5 To 04-Apr-2L0C0I4M1S0I:M4S9:#578 MRM o1f Channel ES- 85 97r7e6a3 Afri sheo rr-srb 7brerer athrTiem | 1 | | 1 t ". Exygen Research, 3058 Research Drive, State College, PA 16801r225a225 49 QSueaanytihtey. s1a7m0p7lse, Re"SpaorrNto.:040304n, Bxt.Dace:04/03/04, AnaiystiK.Risha Page 12 | ME Seatmhpoll de: Lid st: eD P:\r DaCao \CLb CHHSNSSa n ?\aNagsl esllya yanxc\\000088 APFO.PRONSATpLeDB\040304A Filter APFO.PRONSEHDD\031704 PTOA paper oEase t modified: Tus Mar 33 10:5e4:3e3 e 2004 nim pret ven | Whatman 41 Filter Paper Control i - | |100400,3104A-1011 Sm (Mn, 2x2) 04-Apr-2004 11:06:38 LC/MS/MS #7 rea 536. MRM of 1 Cha4n1n3el>Es36-9| 114 1.31e4| |i | o%liran te nAA plier, Time Too abo abo abo | sho | ebo | 700 800 l 1 1 I I| Exygon Research, 3058 Ressarch Drive, State College, 7A 16801n2a26 U050 FR -- po LL manos von | Last modified: Mon Apr 05 06:48:24 2004 | =WEeee mn on -- | Name: 040304A-1012 = | Slotted Filter Paper Spk A, 50 ng/L. 04-Apr-2004 11:23:21 LC/MS/MS #1 |040304A-1012 Sm (Mn, 2x2) | 100- | {+ mr mr 75.73460. TT MRM of 1 Channel ES- | 413> 369) 7.794 TT | | | | | 1 | Sxygen Reseaxch, 3056 Research Drive, State College, PA 16801 + YU 057 Choa? eGuvnmninteytSty spetle seBOpOoSrtLe ousesou/oa/on, msiyaimiaien roe 10 rte She E | aOLe atTslt mamooadilfEnileida:s PRCTATA MoonnRAoprr 607O 5 06:486 a :24B2O00 04L FO.PNEASTSO Pex sermo31n704 310m ~ apa | mtr tm 03 ores os I ---- st0300-101 5m, 2) PPA | Whatman 41 Filter Paper Spk B, 50 ng/L. | 10 | %- = Coiensee 5.35 04-Apr-2004 11:39:5 LC/MS/MS #7 413> 369| | Too . 200 | 300 400 500 r 600ep 70e 0 r800Time | | | I 1 | I 1 serge seseeseh 3048 Hesesees Deir, Thats Sriinge, Th S6EEE : i QSuuaenytioty.is1a2m3p7l5e, ReseprortNo.:040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 14 | mSaemtplseoaLiiksitc: es Hethoa: ePoPn\\DDhaaertara\\LL0G3CHMSSoHHcSSi?a\\zNiMaaazdsss1layyonnsxx\\000088 APFO.PRO\SampleDS\040304A Filter APFO.PRO\MeCHDB\G31704 PFO paper R LastL modified: Tue iadMar 23 10554132 20-_--04 -- _ ----_ aaa"'"'"i'j'znit [semen som sor 03 0738020 2008 | mper:e osor0ana0ne || [1:sioPtFteOdA Fier P-- ap-- er S--pk--C,G 500mnga iLsmeeegee ae ee ls | |0p40y304-1014 Sm (Mn, 22) TM 750350s -| or 04-Apr-2004 11:56:4: MRMofL1CC/hMaCnSan/nefMcleSEleSo-x Areal | To TR ae eh ab sh soo 7m soTime | | | 1 1 \ 1 | ' Exygon Research, 3058 Research Drive, State College, PA 16801 1 u053 C2529 qQUuTeLnErEiS zy suI mgte seREpEoLrtnatn, pesoacero4/63/04, Anaiyatir.isha rave 1 - | Sample List: P:\Data\LCMSMS7\Masslynx\008 mLBoEe nTk meoe dieeiede : MonApmews e0T m8DiesR EioiiLe00S1 Last modified: Mon Apr 05 06:48:24 2004 ome.sroweraoR 033708 708 APFO.PRO\SampleDB\040304A Filter paper | cset ton moe 03 orezness woos [J p-- en | (Whatman 41 Filter Paper Spk D, 500 ng/L. A [I 040304A-1015 Sm (Mn, 2x2) 5sss 04-Apr-2004 12:13:2; LC/MS/MS pa iSee `MRM of 1 Channel ES- | %- ||L To--o zoo abo | 400 | s0: 0 600 700 |er 800Time| | i | | | evpgen Reseasch. 3058 Resasceh Drive, State College, PA 36801 J 054 C2330 -- emNo. 1040304, Bxt.Date:04/03/04, Analyst:X.Risha rage 16 - | Sample List: foeLarlstt emtoidtitfieedd:: P:\Data\LCMSMS7\Masslynx\008 TMEoane EAWparre0555 oS0060:5480V:0i23a43n122y00a00a44\008 APFO.PRO\SampleDE\040304A Filter ABFO.PROVMeLKOBG31704 PEON paper ai =eo ----------TTTE | XC032304-1, 25 ng/L standard 04-Apr-2004 12:30:1. LC/MS/MS #i |CancelOx is printing02x2) MRM of 1 Channel ES- Le Te i1 1.6p9es5| Pols 0 ~ | FE A TTT aw ab i ab sb eSEto 700 sub || | | 1 | 1 Eygen Research, 3058 Ressasch Drive, State College, PA 16801 | L055 n2502 f I ACETER ho. :040304A, Bxs.Date:04/03/04, MmalyatiE.Risha sage 17 = fumple List: F:\Data\uOHSHST\NassLyme\008 ABFO.PRO\SampleDB\GA0304A Filter paver 1| E a eLEalstit anmsodeiifeiead:: MTE eoen Awperr 0B5o 0S6o:eu 4e8va:is2as4s y2F- 0ao0es4\008 APFHO.PRNOHSEhDR\-031704 PEON . -R S | princess on apr 0s 07:35:10 2008 i N-am_e: 040304A-1017 ; i:pPA | L2178-1 ~ 40304-10S1m7 (Wn, 22) b= ooY] Tsosiasits 04-Apr-2004 12:46:52 MRMofL1CC/hMaSTn/noMeSSnl aE#eeS7-d rea i ;| loTrTemT ae abe abe abo ebb 7bo aboTime I i | | | . . | . ~ Exygen Research, 3058 Research Drive, State College, PA 16801 , J056 cnonaz QSiuiatnytiNfoy.rs1a7m1p7l6e, ReSaptorNto.:040304A, Ext.Date:04/03/04, Analyst:.Risha Page 16 SLaasmtplmeodLiifsit:ed: Nathod: MFooP\ni\DDAapatra\t0L5CaM0S\6H:SL4T0\HC:Na2aaH4ssaE2Ll0yyH0nm4xxS\\00T0088 APFO.PRO\SampleDB\040304A Filter APFO.PRO\NetHDB\031704 PFOA paper Last modified: Tue War 33 10a :54133 2004 | mTaemxet:: ososear-sose A. ePOA | L2178-2 | 0p4o0y304A-S1m0(1Mn8, 2:2) 1i [= 12253375276 04-Apr-2004 13:03:31) MoR f1LCChIM aM1nnS3e/>lME#3SS6;-9 3A2re0a = To- o 2bo so: o abo 00 600 700 800Time | | | | Sxygen Research, 3058 Research Drive, State College, PA 16801 | v057 n23a3 QSutaundtyiNtoy.isLa7m1p7l8e, ReSpetortNo.:040304A, Ext.Date:04/03/04, Anelyst:K.Risha rage 19 sample List: Pi\Data\CHSNST\Masslynx\00S APFO.PRO\SarpleDB\040304A Filter paper | VLLaaatsshttodmm:ooddiiffiieedd:: TPMuo7en\DMaAaCprar\L20C35NS10M06S1:754\481H:3a234sa122y000n04x8\008 APFO.PRO\NGEEDE\031704 PFOA I vsoeecmeerr --a--n -- mee 03 oresst0 2T 000 R | TNaamee: 040304A-1019 | n iee s een | I 1% 0403044S1m0(1Mn9, 232) |" TT ibe 300 spo 12458535055 abo 500 04-Apr-2004 13:20:09 MRMofL1CCIhMa1nSn3IeMl3SE6S#9-7) 1.A0r9e8a ove]| po 700 800 1 1 | | \ \ : Exygen Research, 058 Research Drive, State College, PA 16801 . L058 cna A - | Ea Last modified: RESFELSTCSE Mon Apr 05 06:48:24 2004 omemm-- = A OR | L2178-4 | [0440304A-1020 Sm (Mn, 2x2) eee eee eee 04-Apr-2004 13:36: LC/MS/M#S3 534 MRM of 1 Cha4n1n3el>3E6S9-) [- |= | 3 : -- Area i. 1 ; 1 i J059 QSueanytifay ESTamEpRleS ReSpiortNo. 1ot0304A, Bat.Date:04/02/08, Analoxy.Rsishta Page 21 | MESaamtphnlse:dLista: h F B:\DaE ca\\LLD CHHSSHST\aassC sol1y7mmc\000088 APFO.SRO\SsaPLeDE\O40304 Filter APFO.PROVMOLHDBNO31704 PFOA paper e East me oditied: Tus Har 55 30154135 2000 . ELBIOR --------------------------------------------------------Ty | L2178-5 04-Apr-2004 13:53:4 A040304A-1021 Sm (Mn, 2x2) [| 53 8602706 LC/MS/MS #i MRMof 1 Channel ES- Tisrrse00a 5.697 | olTTTsi. T sho abo abo sho eho bo shTiome)| || | 1 1 1 1 1 Bxyaen Seseaceh, 3056 Research Drive, State College, BA 16801 1 L060 2536 QCuranatiyt.y STaEmTp7l0e. ReTSpRorWco. 1040301A, Bxt.Date:04/03/04, Analysts.Risha rage 22 | EL Satmhpola es Lis, t: FPrr\ADraDtaae\LECHaSN\STLW\aNCassHssLIEyyamNee\\S0000?88 APO.PRO\SARpLeDB\O4O30A Filter APFO.PROVMethOBA031704 PFOR paper e Hast moaitiad: TB ue ar 35 20054135 2908 eeeeeeseerereeeeeeeemmeemeemeemees | seimcerr om ne 08 ermine some | NTaomes: 040304A-1022 1. pron | L2178-6 |o40304-1022 5m (in, 252) | "100, | [|= | Too 2h 5.34 15663896 sho : ab. sb -- i. 04-Apr-2004 ates MRMofL1CCIhManSnIeMlS5#.7 413> 369) 1.e27a8| eto zoo sobTime | | | 1 I 1 I Sxygen Research, 3058 Research Drive, State College, 7A 16801 i , . u Whsar oQuayntifWoy. S1a2m2p37l8e, ReSeptorNto.:040304A, Ext.Date:04/03/04, Analyst:X.Risha rage 23 Sample List: P:\Data\LCHSMS?\Masslynx\008 APFO.PRO\SampleDE\040304A Filter paper | | HLaatsntodm:odified: Last modified: MTouDneaAMtpaarz 02\53 L0160::C4584\HW::a2342sSs212M00y00m44Sx\0?08 APFO.PRO\MSthDB\031704 PFOA C--O | aTemxet:: osos0an-rozs Lo oe i 11o: pPrFoOAn SE | [2178-7 |04030471023 Sm (Mn, 262) 7 i | I| Mwd | LL 1.00 2.00 emerges 141s34a675 Te ree SE 04-Apr-2L0C0I4M1S4I:M2S7:#179) MRM of1 Channel ES2008) Area, | 3.00 4.00 5.00 r 6.00 r 7.00 r 8.T00ime | 1 1 1 Exygen Research, 3058 Research Drive, State College, PA 16801 w062 C2308 ole TS "ES nr Te The are APOINTO PhlteE popes | EE Last modified: TNA Mon Apr 05 06:48:24 2004 em Be = | princes: won ape 05 0735010 3004 a.pA | XC032304-2, 50 ng/L standard Lo l[fooost1024 Sm tn. 2 19% ! | |4 04-Apr-2004 14:43:5 LC/MS/MS RM of ane 53s prigh Yet 14481 13165) | } l Te am im r sh F eke r rme abTime | I 1 chest 0063 | SQuhaandtyifNyo.S.a1m2p7l8e, ReSpeotrtNo.:040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 25 | aSasm?plmeoLdiisfti:er Hothod: oPPinr\\DDaaapttraa\\L0LCsCNMSoSHcNS.STd70\\:MN2aa4ssss1l2yy0n0ndxx\\000088 APFO.PRO\SampleDE\040304A Filter APFO.PRO\HGLhDR\031704 PFOA paper I CZesr t modified: Tue Mar 23 10:54e :32 2004 ee Printed: Non apr 05 07:35:10 2004 Texts [ L2178-8 mG 04-Apr-2L0C0I4M1S5I:M0S0:#474) 0403041025 Sm (Mn, 262) 532 MRM of 1 Cha4n1ne3l>E3S6-9| . 10 aon 7.A5re3a4l | | oT p ere rr pre200e | 3-- 00 -- 4005 r 00 E60e 0 7rr .e00rr e 80Tim0e | i | Exygen Research, 3058 Research Drive, State College, PA 16801 1 . 0064 C2340 BQuayntiTty rSaempl,e ReeptortNo. 1040304A, Bxt.Date:04/03/04, Analyst:K.Risha Page 26 = sample List: Pr\Data\LCNSHS?\Masslyn\008 APFO.7RO\SampleDS\04G304A Filter paper a || MLaeaststhtodmmseoddiiffiieedd:: MHmoione aAMpairr \0255 R1006ee1:54e48x:1a23sG42s122H0y00m0es44x\t070e8 APFOi.PROi \NGCi EDB\i 0317o 04 Pn RON o | ! Printed: Mon Apr 05 07:35:10 2004 [| aTemnet:s osososn-aozs Cowen | L2178-9 |040304A-1026 Sm (Mn, 2x2) 100, |- * 23053333354] LCMSIMS 04-Apr-2004 15:17:21 ea MRM of 1 Channel ES- 4131>.3340689) |- |OTe 2ho ho abo spo eb 700 0T0ime. i | 13 | ii i . sxygen Rescaxch, 3058 Research Drive, State College, PA 16301 \ Cnnnea3aAeL V065 | gGuiuvmeersNeor.isLm7o1i78e, nSeetrsNo.:040304A, Ext.Date:04/03/04, Amalyst:K.Risha rage 27 Sample List: P:\Data\LCMSMST\Masslynx\008 APFO.PRO\SampleDB\040304A Filter paper | HeLLaatsshttodmm:ooddiiffiieedd:: MPTu7oe\nDMaAaLprar\L025C3NS01H60S::?45\84H::a2342ssl22y000n04x4\008 APFO.PRO\NGEDB\031704 PFOA on coder ee a- | seinen: Hon Apr 05 07:35:10 2004 | eexmeesr caoromn-zom | (vzeene |1| 5040304A-1027 Sm (vn, 232) [= 26155424908 04-Apr-2L0C0I4M1SSIM3S4#17 MRM of 1 Chanel ES- 4131>.3430689] Area || Tho bo 300 400 | 00 3 600 - 700 800Time | | I Exygen Research, 3058 Research Drive, State College, PA 16801 u066 C2342 | qStuuadty eNro.:sLe3p1t75s, sSeeetrsNo.:040304A, Ext.Date:04/03/04, Analyst:X.Risha Page 28 | Sample List: NLoatshtodm:odified: MoPn:\DAaptra\0L5CH0S6H:S4?8\:M2a4sa2l0y0n4x\003 P:\Data\LCHSHS?\Nasalynx\008 APFO.PRO\SampleDB\040304A Filter APFO.PRO\HeLhDB\031704 PFOA paper Lbaasset modifieda:: Tue Mar 23 10:54:32 2o0r 04 ------ -- ext: | E [2178-1e 1 | 0o4o00304A-1028 Sm (Mn, 2x2) |% 24956370002 E 04-ApE r-2004 1E 5:50:54 LCIMSIMS #7) MRM of 1 Cha4n1n3e3l>34E06S89-]| `real || 100 200 300 400 | -500 600 7.00 8.0T0"ime ; Exygen Research, 3058 Research Drive, State College, PA 16801 l wue/ C233 i QSutaundtyiNfoy.:S1a3m1p7l5e, ReSpeotrNto.:040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 25 :|= STaesmkplmeodLiifsit:ed: Method: M77Po:n\\DDAaapttraa\\L0LC5CNMS0SN6NS:S7478\\:Ha2a4ssssl12yy0nn0xx4\\000088 APFO.PRO\SampleDB\040304A Filter APFO.PRO\NethDB\031704 PFOA paper gLoanstcomdoedrified: Tue Mar 23 10:54:32 2004 EE Ea I princea: Mon apz 05 07:35:10 2004 | Tmeamxet:: os0304r-2028 mea | L2178-12 - 1040304A-1029 Sm (Min, 2x2) - 100 255392600 04-Apr-2L0C04IMJSsIorM#aSd MRMof 1 Ch4a1nn3e>l E3S69-| 1.A3r4eea8l [I |: ( - 100 2.00 300 40 500 d 600 7.00 Time | 80 t i | 1| i| |i Exygen Research, 3058 Research Drive, State College, PA 16801 | U068 nena QauayntiWtoy. s1a7m0p7l0e, ReSpeotrtMo. :040304A, Ext.Date:04/03/04, Analyst:X.Risha Page 30 E - HaSatmhpolde:E List: 7oP:A\DA Datea\CLCaMS\HSL7C\HMaHasssEs1lNyynaSx\\T0000\88 APFO.PRO\SampleDB\040304A Filter APFO.PRO\NLHD3\031704 PFOA paper dToebstcomogdeirfied: TueTNEar 73 10154132-- 2008 _-- [pr-- e -- Leen | L2178-13 - | . po|040304A-1030 Sm (Mn, 2x2) | %- | oi wo . bo 22851303730] 300.)ha apo 500 04-Apr-2004 16:24:21) LC/MS/MS MRM of 1 Cha4n1ne3al>t3EeS6-s9 Area soo 700 re 800Time| | 1 | | Exygen Research, 3058 Research Drive, State College, PA 16801 | ul69 n25845 | QSuhaunetyifNyo.S.a1m3p1l7e, ReSpetortNo.:040304A, Ext.Date:04/03/04, Analyst:X.Risha Page 31 | Sample List: P+\Data\LCMSNS7\Masslynx\008 APFO.PRO\SampleDE\040304A Filter paper { ~ MLLeaatsshttodmm:ooddiiffiieedd:: re MPTioune\DAMapacrra\02L53CH01S60M::S45\84M::a23s42s-- 122y00n00x44\008 APFO.PRO\- HethDR\031704 PFOA oo mex: | [|L2m17e8-14 | 0p40304A-1031 Sm (Mn, 262) | 26915020708 G 04-ApA r-2004T1e6:d41:08 LCIMSIMS #7) MRM of 1 Cha4n1n3el> E3S6-9) 1."3A4re0a8l ;i Iq 1.00 200 300 400 | 500 600 7.00 800Time | | Exygon Research, 3058 Research Drive, State College, PA 16801 | 0070 C266 {quantity sample Report sage 32 I Study No.:L2178, Set No.:040304A, Ext.Date:04/03/04, Analyst:K.Risha | ESaaatmthpolame:odLsitsit:cs: wePA:nb\aDibaotara\\0L3LCHoSC6HiSiN?a\M:MEadastsNss1alSytym0nsxxT\\00\008SAAPPFFOO..PPRROO\\SNaSmHpDlBe\D0E3\107400430P4FAOAFilter paper --, Yast modified: Tue YoMr ar 3333 10154137 2004 E -- - | oTamxee:s osososa-sosz |3 iC. 0PsR2O3M043, 160 ngiL standard 040304A-1032 Sm (Mn, 2x2) i=% 100, 16S231449 CL Tho 200 soo| ao0 | soo [= ' . . \ . . 04-Apr-2L0C0I4M1S6I:M57S:#5 MRMof1 = Cha4n1n3e4l>5E63S66-9)) 600 zoo soo | Bxygen Research, 3058 Research Drive, State College, PA 16801 t , u07i nea? [|| qGuueasnttsisfyaSmapmpllee ReBepmenotrrt eson, xt.oacesas/o3/08, malyetik.mish Page 33 | - dma PSLaarsmttplI menodLiifssit:eE de:TMPso:en\DwaAtpraE B\L0EC5uMgaSi 0uM6sS:t74d\8h:Ma2ao s4sssgil2yy0on0ex4e\\0000880AAPPTFOO..EPRROO\\HSAaCmHpDlEeADOB3\1074000830430A708Fi0lter0pape0r 0 I primes tom a 05 on028010 2008 | freaemss sesoenons [| os mies DE ee-- e -------------------------------- eet--T-- l | L2178-15 04-Apr-2004 17:14:3 LCIMS/MS #i 04030!,88.1033 5m (4, 22) wil MRM of ChomneWleLe5.d | % | TT aw a ab sey e abyo 7h srmime | Bergen Resensch, 3058 Raseazch Drive, State College, FA 36303 | RL 1 i | QSuiatndtyiNfey.1S1a3m1p7l5e,ReSpeotrNto.:040304A, Ext.Date:04/03/04, Analyst:X.Risha Page 34 Sample List: P:\Data\LCSHS7\Masalynx\008 APFO.PRONSampleDB\040304A Filter paper | LMLeaatsshttodmm:ooddiiffiieedd:: MPTouien\DAMaaptrra\L023Q5HS01H60S::745\84::H32a24ssl22y00n004x4\008 APFO.PRO\NOEHDE\031704 PFOA [Eee | seinen: Mon apr 05 07:35:10 2004 Texts | p 2178-16e [I- 040304A-1034 Sm (Mn, 2:2) : 100,1 i | L Sherrer100rep20e0 |r a00 pa 04-Apr-2004 17:31:1. MRM ofL1CC/hManSn/eMlSES#- 20957362000 1"31>343e669)) `Areal 400 500 60: 0 7.00 500Tine| ) Exxvygen Research, 3058 Research Drive, State cCoorlelge:ge, PA 16801 v073 i n92819 || qStuuadnytiNfoy.:SLa2m1p7l8e, ReSpetorNto.:040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 35 Sample List: P:\Data\LCSHST\asalynx\003 APFO.PRO\SampleDB\040304A Filter paper | HLLoaatsshttodmm:ooddiiffiieedd:: PTM:uo\enDaMAtaparr\L023C5NS10H06S::754\48N::a3224ssl22y00n00x44\008 APFO.PRO\NethDB\031704 PFOA [ ----domcoder-------- Printed: Mon ApE 05 07:35:10 2004 || NTaamxe:: 040304A-1035 | 1: ern _ | . 1217817 04030441035 Sm (Mn, 262) [fe11 | % | oC 100 200 3.00 co ---------------- 04-Apr-2L0C0I4M1S7I:4M7S:5#1) MRM of 1 Channel ES- sa Toe 26965266] 1.r3e4a0l8 " 400 500 600 700 800 | i 1 Exygen Research, 3058 Research Drive, State College, PA 16801 u074 . nen50 | QSutatndtyiNfoy.:SLa3m1p7l3e, ReSpeotrtNo.:040304A, Ext.Date:04/03/04, Analyst:X.Risha Page 36 | SEaasmtplmeodLiifsitc: a: Kothod: oPn:\Dhapta\0L5QH0S6M:S475\:M2a4ss2l0y0n8x\008 7 i\Data\L\MCagHsLSynHx\S00T8 APFO.PRO\SampleDB\010304A Filter APFO.PRO\NOthDE\031704 PFOA paper ----JoLbas-tcomotdeirfiad: Tue Mar 23 10:54:32 2004 Text: | L21e7e8-r18 104030441036 Sm (Min, 2:2) 100;7 [=i | - Od1er | 100 | 2.00 04-Apr-2004 18:04:27 LCIMSIMS#7 2315132108 MRM of 1 Cha4n1ne3"l>13E34S66-08) rea 300 400 500 600 70. 0 800Time i | 1 0 ' ' t Beygen Research, 3058 Research Drive, State College, PA 16801 . u075 CN2R5% | Samp Liss ELE Last modified: MoNnonAEpE Er 05 06:4E8:n 2L4 E20E048an o9r8oPsaOaNnaSnAoSsSAO31Pier pes mame semsmamene wm ___ | L2178-19 - L[: f|040304A-1037 Sm (Mn, 2x2) | i 0 To ee er EwSi:e sh 04-Apr-2004 18:21:01 MRM LC/MS/MS iiex of 1 Channel ES- Re T Time i 1 Exygen Research, 3058 Research Drive, State College, PA 16801 0076 CnO85R | QBuayntiTfy7s3a7mp5le, ReSeprortNo.:040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 38 | TamaptlemodLiifsti:ed: Natnoar MBPoRrnD\DAeaptraL\0L5\CH0LS6H:SG47\8\M:HHa2as4sEssl2lNy0yn0nx4xS\\00T0088 APFO.PRO\SampleDB\040304A Filter APFO.PRO\MetHDE\031704 PFO paper hYrast--modi-- fied: TusEMEar 23 10:54:32-- 2004 ---- BMN ' Printed: Mon Apr 05 07:35:10 2004 | aTemxet:: osos0en-2038 wewen 1 [Lz1r820 |osososn-toss Sm (Mn, 2x2) t 300 - ' * saz 22281124] CLe T o p shoe : sho ae bo sto eo 04-Apr-2004 18:37:50 LC/MS/MS #i MRM of 1 Cha1nn3e>l E3S6-9 "1 3468] Area | Time me | rho 00 [= t | \ ' . \ : Exygen Research, 3058 Research Drive, State College, PA 16801 \ cri | | Ee. Quantify Sample Reopmoerhto. 1040304, Bxt.Date:04/03/04, Analysti.Risha Page 39 | HE Seatmnpolde:L List: wEePe:a\DAeCe\U\CHsSNxSt?\adNEsagsis1iNyynnmec\\s000088 APFO.PRO\SspleDE\GA0I04R Filter APFO.PRO\NSCHDS\G31704 PFOA paper coYagst emodnifited::TTuona tMaart 33 1T03:1540:333 2004 ---- | eTemnetrs cassoancsons 1: POA |~ L2178-21 Lf,|040304A-1039 Sm (Mn, 2x2) 2esstiaorz 04-Apr-2004 18:54:41 LC/MS/MS #: a orel MRM of 1 Channel ES- | %- | Th ab sw ab. o sto abo 700 8boTime | | | | . \ : \ Sxygen Research, 3058 Ressarch Drive, State College, PA 16601 crnaza 0078 | | ouncssE y sampE le Reporrte. c040304A, Bxt.Date:08/03/04, AnalystiK.Risha Page 40 sample List: Pr\Data\LCMSST\Masslynx\008 APFO.PRO\SampleDB\O40304A Filter paper F1| | SMeeLatishtodma:ostdsiicftiioeeadr::sTMP:uo\snDaWAtaparr\L20C95MS10N06S1:754s\48M1:ea3234srsl22ey00n00x44\008 APFO.PRO\MethDB\031704 PFOA -- | pwrmae:t osos0sn-r000 | Coen Xcus2s044, 250 ngiL standard |040304A-10S4m0 (Mn, 2:2) |: | " sas 211881 04-Apr-2004 19:11:28) MRMofL1CCIhMaSn1nI3eM>lS3E#6S79-))| 1.776| Area | : . IE 200 300 400| 500 60r 0 r 7.00 r80Tim0e i \ : \ \ Sxygen Research, 3058 Research Drive, State College, PA 16801 u wus C2455 QSuvaanrtiHtby. s2a0m7pl3e, ReSuprorMto.1040304, Bxt.Dater04/03/04, Amalyst:K.Risha Page 41 Sample List: P:\Date\LCHNMS?\Masslymc\0DS ARFO.PRO\SSEplsDE\G40304A Filter paper | -SaMLLneaatasshttnodmmasooeddii,tffiiieeeddd:: TMPTouooesnnrAWtaeparrr\0359L01m60C:145084H:1ua23s43Ss122y00H0a044r\--0S08 APFO.PROVMLADE\031704 FOR pred 1. pron | L2178-22 [of [. a040030)4A-1041 Sm (Mn, 2x2) a 04-Apr-2004 19:28:16 aessosaonas Te LCMSIMS#7 MRM of 1 Channel ES- | iireeayl | To zbo abo ab. o sho eho 7h aboTine | Sxygen Research, 3058 Ressarch Drive, State Colleg"e, PA 16801 C2456ubol SQueaanytitNoy.1s2a2m3p7l8e, ReSpetortMo.:040304A, Ext.Date:04/03/04, Analyst:X.Risha sage 42 | pSamrplee sListt:o Method: 7sPornA\DDhartaak\oLFaCcH\SeNLStTCr\MiMHaiasdsSss10lNyydnnSXx\\T0000\88 APFO.PRO\SempleDB\040304A Filter APFO.PRO\HOtHDB\031704 POM paper | lamsetemdodtified: Tue Nar 2 23 10:54:32 2004 - _-- EE -- [J exer Leween | : L2178-23 040304A-1042 Sm (Mn, 2x2) |": Cd [I i oTTt T 2b: o abo [5 28s7n05 me 04-Apr-2L0C0/4M1S9/:M4S4:#59 MRMof 1 Channel ES3.`60r6e7a 40 soo | 60 |--7o0 800Time|| 1 1 | | \ \ \ \ Exygen Research, 3058 Research Drive, State College, PA 16801 \ . 0081 C2587 qSuaanytitNYy.TsEaTmrplae. ReSp0o8rtNo. 10403048, Ext.Date:08/03/04, Analyst:X.Risha rage 43 [ BVaaamtphlioed:Lliste: PwBr\iato ra\uLbOea UuMrSYe\TMaeasssa a1aLyrnAx\ \\000088 ABFO.FRO\SampleDBAOAO304R Filter ABPO.FRO\NSCEDEA031708 PEON paper [EEastRmodE ified: E Tie WarE 35 10:5E 4:35 2008 srintea: om apr 05 07:35:10 2008 pre en | L2178-24 I | 04030441043 5m (Mn, 22) -i | Yr sit5i3e2s 04-Apr-2004 20:01: MRMof L1 CCh/aMnnSe/l ME#5S;41a3s3e6r9 Area | T abo st; o abo soo - | sbo : 780 so"Tine Bxygen Reseach, 2058 Ressazch Drive, State College, PA 16801 | 0082 "2558 ii || " QuantiTfy sIampEle ReTpeorNte. so40304n, Ext Date:04/03/04, Analystik.Risha Page 44 [[I| SLaasmhplmeodLiifsit:ed: MBBoi\n\oDAapatraL\0L5aCH\0S6HL:S4TC8\H:UMa2as4Esss12Nly0ym0Smx8\\T0000\88 APFO.7RO\SaapleDS\040304A Filter APFO.PRO\NSERDB\031704 PFO paper ~ Loahstcmooddeifsied: Tus Mar 33 1f0:5i4:37g2004 o _ [ppTeed I OA eee ----------------------------------ee | [L2178-25 04-Apr-2004 20:18:24 04030441044 Sm (Mn, 22) MRMofL1CC/hManSn/eMlSES- | . ) 100, }i arz5r3a1no 413"4>A33r06e09a7) |* | T0200 300 abo 500 edo 700ee800Time 1 | " |i Exygen Research, 3058 Research Drive, State College, PA 16801 i v083 C7059 SQtuaundtyiNfoy..s1a2m1p7l8e, ReSaptortNo.:040304A, Ext.Date:04/03/04, Analyst:X.Risha Page 45 | mSaamplmeodLiifsit:ca: woPnr\Dhaotra\oLbCHoScNsStTe\iMiaissal0y0ntx\008 APFO.PRO\SaspleDS\040304A Filter paper MaLatshtodm:odifisd: TPuAePWAaLr\L3C3HS1N0S:75\4N:a3s2s210y0n4x\008 APFO.PRO\MGEDE\031704 PFOA eet - | pnarmee: osos0sn-soss tow0 el | . 2178-26 04-Apr-2L0C0I4M2S0I:M3S5#:170 [+040304A-1045 Sm (Mn, 2x2) | eei] |1 | LL 100 200 | a00 531 4582807 abo 500 MRM of 1 Cha4nne1>l 3E63S-9 3.A8r5e7a | 600 700 800Time| 1 1 i| |i ! Exygen Research, 3058 Research Drive, State College, PA 16801 \ . 0I 084 C2560 J SISSY SUE, S PO cuosoun, mespaceroa/ea/ot, ansiyetin.nisha ree ac |RE m5Cia:rteeIsertiraEtp y Sbeg RE aPCg E ve rN5S AO ASREENAALES FibtoE reresven S708 Tare? neSE SR oo | reas won nse 05 avvmeenn 300 1: PFOA EE | L2178-27 Lf oa0s04a-1040 Sm in, 2) 5% C1 1 4082124 [+ || : L1 200 0 300 40 00 500 -- 04-Apr-2004 20:51:47, LC/MS/MS #7! a piopl 3.62e7| rere reer Tne 600 7.00 8.00 i/ | voen Resensch, 3058 Rasaazeh prive, State collage, 7A 26801 | 008s cnet {i SqtuuadnytiNsoy.:s1a2m1p7l8e, ReSeptorNto.:040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 47 | - SLaasmtplmeodLiifsit:ed: MPo:n\DAaDtEa\0L5CH0S6M:S4?8\:M2a4ss2l0y0n4x\008 APFO.PRO\SampleDB\040304A Filter paper | dLoatshtodm:odified: \TuDeatMaa\rLC2H3SI1S07:\54K:a3s2s12y0n0x8\008 APFO.PRO\MethDB\G31704 PFOA --gebeoder | Tmeexmte:: osososa-sos peweor | [2178-28 |: 04[0e3e0)4A-1047 Sm (Mn, 2:2) |: %i 0 10309608 es 04-Apr-2004 21:08:2! LC/MS/MS MRM of 1 Channel ES- 9.A4r7eea7l | LTT v 200 300 400 500 600 7.00 80Time | Exygon Research, 3058 Research Drive, State College, PA 16801 | 086 C2562 QStuuadnytiNfoy.:S1a3m1p7l8e, ReSpeotrtNo.:040304A, Ext.Date:04/03/04, Analyst:K.Risha rage 48 Sample List: P:\Data\LCMSHS?\Masslynx\008 APFO.PRO\SampleDB\040304A Filter paper | MLeatshtodmsodified: MPo:n \ApDr a05ta06\:4\L8:aC2s4HsL2Sy0n0Hx4\S00?8 APFO.PRO\NGthDB\031704 PFOA ~ JLasot mbodcifi--oed:dTeus rMar 23 10:54:32 T2004 | aemxet:: osos0sa-s0ss | Come Gress |~ oasoaniods sm an. 2:2) 100, * 532 _ 8861053 04-Apr-2004 21:25:01 MRM ofL1CC/hManSn/eMlSES#- 413>369 8.0A1ree7a| i -- ro | or 100 200 300 400 500 600 7.00 8.00 | _ Exygen Reseszch, 2058 Research Drive, State College, 7A 16801 i wus? 0003 QStuuadnytiNfoy.:s1a2m3p7l3e, ReSpetortNo.:040304A, Ext.Date:04/03/04, Analyst:K.Risha Page 49 Sample List: P:\Data\LCHSMST\Masslynx\008 APFO.PRO\SampleDB\O40304A Filter paper | NLoatshtodm:odified: MPo:n\DaAtpar\L0C5HS0H6S:T48\:a2s4s12y0n0x4\008 APFO.PRO\MethDB\03L704 PFOA ~--sLbasotomotdiefised--: ~ Tue Mar~ 23 10:5P 4:32M2004 | aemxee:: osos0sn-zoss EN. | XC032304-5, 500 ng/L standard -- 04-Apr-2004 21:41. 4 LC/MS/MS #: 040304A-1049 Sm (Mn, 2x2) | 100 84523521 MRM o1f Ch4an1n3el>3E6S-9 8.4065 rea | | I | * | 0" 0 20 | 300 0 400 500 600 700 800 Exygen Research, 3058 Research Drive, State College, PA 16801 | uoss C2564 | ample Lise: Last modified: DMi oo\nonAepari0c5ke06:a48l:2g4 n20e04\oAe5. PONE DRA 08A Ter pes ee i | 1XC032304-6, 1000 ng/L standard I (040304A-1050 Sm (Mn, 2x2) [= ERR--y 04-Apr-2004 21:58:3: LC/MS/M#S: 5%532 MRM o1f i=r Channel ES41 >3639) | TT Tre ma- e ee aTime Exygen Research, 3058 Research Drive, State College, PA 16801 | . uos9 CN2465 [ won [ pr--ftthf po FdiEr y. iE ET r ) ey EER em, :| g g ! | Te Ey . u09%0 C7O006 [ ?) OBES ad i 3)5 IGE w SED z I L- g E i Ey E H[ilge;e FM == H = Of & 1 [IRE EY d| iHsh tk: 48] Z| 1 2 : a Pli Y aaa CoI- ; &IL [FE Ble LNEERECECgEl ENE1$ aly EB Hi Log {3 2 L : s HE I Kk Tee] $143Nd i4 P81 Het | |Co 3 o RAE [300s Ha o1a3d 2I5 HlugRleElggEleElaLlEqeE(2RHfEeIlP:R Icl E3 =3 H PREI RjEN REL 4; I 091 | C0567 BL o. cattesn SER0EED sORDEERsesusts CT LIER TeTssmesssmmssmemssstmmmsssssesess EER ERERE RRRR RRRRRRNRRRERER ER ER RR ERE || Foon . |3 |: ; 1 o0s28888 00m m Bie 088 |- g 1 i (HIEEEE spel sescfosassl ssfiosssncss Besnsasaly &| z | a | 2 EE EE CE Ee nanny gaan : 2 ences : i i ti Hl Eiga se sad saailatitiingiey | 1 dea I 8 Z ERE ERE EERE EEEEERE EEEEEEEEE EREEE EE NEN] [ |5 En [si hgiwmf d ai n y n i y 5gssasss i [EE ZB ruoveeNRn 2RNo NRnrTRN EANR RRST EwIe BIBe BEBt on | i J] | i I TNPTeerea TNO R2RINRINRNERRRNA3885889FY u092 C2468 [ ai.l rloe | ob{| | | 3vi | Z2z g 8 re l| y3 x2 | g T veeeccersessecosseseervessosseesssszaneans | P4EGRd sBoBd oEnREnSdoIndRsd IdsEedIeEdsAadTsAadinEnid nisisisd isiaiastd taazisiziasssidscsiasrsdisisidsidsisisc | 1 | | k3L3 S BEHELa IBMERMBISMBsMsBsRMER INSEysReT S E: d2 8sEzssEzsessassessssscsssszsssessaszazzza: | |2 85 | TNmeTeerea IN Re PPR NNRI RARER 093 N38S5839TY | C2069 | I - | | L | |- l | | LC/MS/MS SYSTEM AND OPERATING CONDITIONS Sponsor Protocol No: NA Exuen Study No: 12178 Instrument compen Software: MicromassQuatzo Ultima (LCMSAMS Unit #7) coMPAQProesioml WorksaiAonPO Microsoft Windows NT: Vers4iBuoilnd 1381: Service Pack Micromass Limited: MassLynx 3.4 Build 004 HPLC Equipment: Hewlett Packard (HP) Series 1100 HP Bin Pump HP Vacuum Degasser HP Autosampler HP Column Oven HPLC Column: G(eJneOsiNsEC-S8,CSHcRmOxM2.A1 TmOmGi.Rd.AxPP4aHptYN(Eo:x,ygFeRnSI9D6:2MEA)13296) Mobile Phas (A): 2mMAmmonium Acetate in TypWateer ol Mobile Phase (B): ~~ Methanol Yule? Avalyst KarenRisha E3Ph0xo3yn8gee:Rne(sR5ee1as4re)cah2rc7Dh2r1i0v,39StFatAeXC.ol(l5e1g4e), 2P3A1.116558001 NOTE: The next 3 pages are computer generated printouts from the masslynx software program. The pages contain the instrument settings used for the analysis of this data set. All Handwritten Peak ID's by: _ || . un0294470 i | Scanning vathod sepors i [7 MaestthoMdodified: vCe:\dMAMSaSrLY2N0X\1060:80A7:P5F0O.20P0R2O\ACQUDB\0DS ROR | eminea: Tue Apr 06 15:26:34 2004 4 | solvent Delay (mina) : 0.00 = Function : 1 MR of 1 ass Fair ( ESP-) {IntSSpteaanrr.t (CTihDnaaenlnteo(lnsMiDen) lsa)y:( Secs ) : 000.l.000003 I| Ronedpeaisnse ( ins) 31 .00 Channel Farent Daughter Duell (Secs) Coll Energy (ev) | 1 413.00 369.00 0.20 10 Cone (V) 10 {| i . . u095 . [fefsors [J r= aerate sods proseatses -- [-- nsday, Aprid 08, 2008 15,3808 oe TE TT Rey | z I r HP1100 LC Pump Initial Conditions [fone "Flow (ml/min) 220.300 BERhBE 2, | OevreanesTem1p5erraetmuyrexRvitgthtet(=Ci)seabie 350 | The gradient Timetable contains 10 entries which are RE, | The Timetable contains 4 entries which are : {sme svomegzen seins onions [Emme Se [RE : i 1 ! , uun9o6nToR | muning method report Page Mothoa C:\MRSSENON008 APFD.PROVACOUDR\PEON TUNE [J uo ape 08 18:20:11 2008 [ema | scoopmsetsiner( mse-) Coferxeapote 1 | _ erSoeeurrstceutreBel]:ock Tem. | Satie" Temp. ~ | ressures [ "anE alyn ser Vacwm i || \ \ ' s2e0t0 rzoan o1l olo00o 02 00 300 Ra oreeens Aoamatnyesse:s TonReersoy1 1 EnStt airtanece fi Bes 2 wHTMientRaierssatyi2e2r Gas Fiows SCeosneeivcaatsion S1e4t.0 man 1i0 5Po0os.n fio 1pF4.dH0 sax aEs.s2 our Cnes73 | XYr8 eFesraRrrEciSEARCH | sInpeocrtdreormteotevreruisfiynmgaasNsarle/sRoblu/tCisont,unaicnaglisborlauttiioonn.chTehcek tisunpienrgfosorlmuetdioonnits hienfmuassesd onto the mass spec using an infusion pump set to a flow rate of 10 uL/min. An | MS scan from 80 to 1380 amu is performed on both MS1 and MS2. The calibration is considered acceptableifthe following 11 peaks are found within + 0.2 amuoftheir known masses on both MS1 and MS2: 84.9, 132.9,172.8, | 322.7,472.6,622.5, 772.4, 922.3, 1072.2, 1222.1 and 1372.0. Ifanyofthe `amnaostsheesar rcaeloiubtrsatiidoen tchheeck0p.2erafmour,metdh.en the instrument will be recalibrated and | `The printouts from the calibration check can be found on the following 2 pages. | 8 Ap | I | , , t REesi0 RTeaon, usa 098 T. 600.281.3215 , COB in |~ EgEpg------------------------ 3 g1 #gis 8 Lo : 51 FR 1E 1 i HE ;5 | 5 [ | 0%# a 2 : EE F H | |g : lg Lo 3 I 55 2s 2 8 y ol Es1t =3 ey -- og 3 3 HI---- lg: | &5 8 iss = =F 3 7 Borg rBge 0 *% EET Cee == B Og gr ---------- } 00C 99 T(oRaeRT; [l- 5B8E2 ge ~ ]S lg 1 T% oo of bi SEE [| Plz aE_ |2 s 35k > 2 EE J II s| Lg 2 | ) g2 E3a5 CE8 Ry 5 : 5 or2 ER - &3 = 8 . 2<8 rs CES | z5 ~ 85 --8 ' 1 -- E-- Ss . --a ~ 8EL g MN Cf i -cgEr 0 TRge } 8 >LE- = ree =| i| ee --------=8 vi00 Chane i i | | | sien Initial Calibration Curve | ExtaS ceioenDouE. n0E40t308 ep, tp |[| . Etmimtseon tceoThecoet fdetof ee ran h Sate) a | o sSagenaConl cens tlarl"ation ares | Lo e me mmom XCoM05043 100 14633 [------ E o. EEfEreEe FEi 2be 0985 gre.110 ot CoeffofiDecterimienatniotn: lstomech 0999889312 An ! ANOVA |Cae Bm == P TmS e --TTST i XVariable| 1406153656 0397684 1900885068 4SOMGE09 1ISS6IS2R 142669207 13S6ISD 14266907 { ; ; ii 97 01 C~raann? eovsvenetans riseAHEETWaasLomI APROFRNAOOO. | Last modified: Wed Apr 07 08:02:24 2004 | Lomel e comparmm amerrron esis | Response type: ExternalStd,Area Coefficient of Determination: 0.999725 - [Calibration curve: 138.596 * x + 996.314 oo Curve type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None. |. |i Thibes Pope Weald -- I ; | | - Respon: | I | | . | 50 2060 2000 | ee | 000 060 To008" Cnn2eam78 0102 [-- = QCueanatihfey. S1a5m0p7lse, Re"SpaocrNto.:040304AR, Ext.Date:04/03/04, Anslyst:E.Risha Page 1 Sample List: P:\Data\LCHSS?\Masslynx\Q0S APFO.PRO\SampleDD\OA0304NR Filter paper | HLLeaatsshttoedmm:ooddaiiffiieeedd:: TWBueoadaMAbaparr L3037 10u08S::T6042::a31p30e 02y0a00s44\008 es _ APFO.PRONMOSADBNO3ETO4 visETa JFrFON amen - Printed: Wed Apr 07 08:04:00 2004 Run CAEA0UAR- S61T0_OUOSGUAR 302 To | "1(Coeae0o5n045, 0 ngiL standard 06-Apr-2004 10:53:0 i Lo 040304R301 Sm (Mn, 262) se MRM ofL1CCIhmaMnSneIlM>S3E0S-9 - ED Tireeda |" | IPAMAN rtA Atma) -rent I BT ah aw ae sh ererlrmrere amTime - | | Buygen Research, 3058 Research Drive, State Collage, PA 16801 i { ui03 [lolatoed:} ||" oauaRntiay seemp.ie ReSpe.orct os00aan, mxc.Date:04/03/04, Amalystik.Risha Page 2 Sample List: Pr\Data\LCHSHS?\Nasslynx\008 APFO.PRO\SampleDB\040304AR Filter paper | Last modified: Wed Apr 07 08:02:10 2004 i bcMeLatoshtodsm:odifoiend: TPTu:ea\DnaMatran\L35CMS1M0S075\4M1a37ssl2y0n0x4\008 APFO.PRO\MethDB\031704 PFOA. printed: Wed 2px 07 08:04:00 2004 | sTeemnes: osos0smm-soz | Cowen |XC040504-1, 25 ng/L standard |Lo040304AR302 Sm (Mn, 2x2) - Gy 06-Apr-2L0C0I42M0S:I0M9S:4#79, 513 MRM of 1 ChapninerleES- | 4634 p"Aerea || " | + T02+ 00 500 |+ 40 Ee Ssoo e 6bo 7e 00 00Time - | r | - i |i _ Bergen Research, 3058 Research Drive, State College, TA 16801 |i . d.id "Nn80 | | ouantia ty saE mpe Reeep.orot s0306an, Ext.Dat:04/03/04, Analystix.Risha Page 3 | NmSeeatmhepolames Leisst:t PwePar\d\DDAeaCrtaa\\LoLCCaHHSnSHUSSeTTn\\HMiaaessss1alyylansxx\\000D88 APFO.PRO\SampleDB\O4O304AR Filter APFO.PRO\NSLRDB\031704 PFOA paper STeabctcomdoedrified:TTeuaMMaEr 3E5 10:54:37 2004 ee ------ | Tmeaxmtes: oavsounz-s0s ETE HE _ | |[XC040504-2, 50 ng/L standard - 0"40304AR.303 Sm (Mn, 22) 8501846 { %- ---- 06-Apr-2L0C0I4M2S0I:M2S6:#24 MRM of 1 Cha1nn5e>l E3S6-9 6.A9r6e0a4l | Oem Too 200 --s--b--|----a--bo-- EE500 TreTbor rr 700ere 800Time| . Exygen Research, 3058 Research Drive, State College, PA 16801 | I. 01g50c51 - l [7 gStaudmyeNso.isL2m17e5, seSewterNso.:040304AR, Ext.Date:04/03/04, AnalystiX.Risha Page 4 sample List: P:\Data\LCNSHS7\Maselynx\003 APFO.PRO\SampleDB\040304AR Filter paper | HLLoaatsshttodmm:ooddiiffiieedd:: WPT:eu\deDaMAtaparr\L20C73HS10H08S::?05\24H::a13s02s122y00n00x44\008 APFO.PRO\MSthDE\031704 PEO Je ee eee Printed: Wed Apr 07 08:04:00 2004 | Toemxet:: ososouan-s0s | am ) [XC040504-3, 100 nglL_ standard | 110040304AR-304 Sm (Mn, 262) r | 06-Apr-2004 20:43:20 LCIMSIM#S 512 MRM of 1 Cha4n1ne3l>E3S6-9 14633 1A.r3e3a5 I 100 200 300 | bo | 500 6r 00 e 700 r8e 0T0ime| l i . - Exygen Research, 3058 Research Drive, State College, PA 16801 . 0106 CnRn8R QSuiannatyitnoy. S1e1m3p17l3e, ReSperorNto.:040304AR, Ext.Date:04/03/04, Analyst:k.Risha Page 5 ample List: P:\Data\LCHSMS?\Masslymx\008 APFO.PRO\GsmpleDS\040304AR Filter paper | LMLeaatsshttodmm:ooddiiffiieedd:: WPTeeu\adDAMACaparr\L30C37HS10N08S:1?05\24H:1a1303gs12200y00m48\008 APFO.PRO\NELEDB\O3L704 PFO ion coams EE _ _ _ | Nparmeet: 040304AR-305 1: PFOA | |XC040504-4, 250 ng/L standard Le |||04010,304AR-3S0m5 (vn, 222) - |" | Tie 200 aoybo aisrire 4b soo eee 06-Apr-2004 21:00:0: MofR 1LCCh/aM MnTSn3/e3MlS6E9S)-| 3.A0r8e6a5 600 700 | 80T0ime r | meen Researen 3058 Rasasseh Brive, Sta College. 5h 36801 ! 5107 n2383 | SQtuuadnytiNfoy.:SLa2m3p7l8e,ReSpeotrtNo.:040304AR, Ext.Date:04/03/04, Analyst:X.Risha Page 6 Sample List: P:\Data\LCMSMS7\Masslynx\00 APFO.PRO\SampleDB\040304AR Filter paper | MLoatshtodm:odified: WPe:d\DaAtpar\L0C7HE0M8S:?0\2H:a1s0s12y0n0x4\008 APFO.PRO\NethDB\031704 PFOA ~-- dLabstcomdoedified: Tue Mar 23 10:54:32 2004 I-- Text: Lo][am J [XC040504-5, 500 nglL standard . 06-Apr-2004 Fie LCIMSIMS 1o0o40304AR-306 Sm (Mn, 262) 514 MRM of 1 Cha4n1n3el> E3S6-9 | - 70740 6.3A6ree5a,l | : 1.:00 200 3.:00 abo |500 6.00 7.00 800Time - i ~ | | | | Exon Raseazch, 2058 Research Drive, State College, PA 16301 1 L108 Crate |"[| OquRanEtiTfyisaa0m5e. ReSeptoo.rtc040304nR, Bxt.Date:04/03/04, Analystix.Risha Page 7 ~ Semple List: P:\Data\LCHSHS?\Masslynx\008 APFO.PRO\SaspleDB\040304AR Filter paper | a LHLaaatsshttodmm:ooddiiffiieedd:: WPToeu\deDaAMtpaarr\L02C73HSH01S80::05\24M::a13s02sl22y00n00x44\008 APFO.PRO\HetHD3\031704 PFOR | sTuemxet:s osoz0amn-20 | Ti LXcooe aEosAod-s, 1000 ngiL standard - 040304AR-307 Sm (Mn, 252) |I - | . T0200 500 SE 185.115447 4p0 500 06-Apr-2L0C0I4MrSiIMS MRM of 1 Cha4n1ne31l>.2E2366S69)| rea 600 700 800Time| _ Exygen Research, 3050 Research Drive, State College, PA 16801 0188 Bocas || oStuuadnytiwf3y.:s1a2m1p7l8e, ReSpeotrtNo.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha rage 8 Sample List: P:\Data\LCHSHS?\Masslynx\00 APFO.PRO\SamplaDB\40304AR Filter paper |1 eLatshtodm:odified: WPe:d\DaAtpar\L07CHS0H8S:?0\2:M1a0sal2y0n0x4\008 APFO.PRO\NOEHDE\031704 PFOA [iLeast modified: =Tue Mar 23 10e :54:e32 e2004 ec te------------------ Printed: Wed Apr 07 08:04:00 2004 [p---- Texts [|= 1erm | (kCo405047, 250 nolL. check standard ~ 040304AR-308 Sm (Mn, 2x2) | 100 ] | | Lr 100 20- 0 300 513 $510 400 5.00 | 06-Apr-2004 21:50:1 MRM ofL1CCIhManSnIeMlSES#13> 369) 3.0A6re8a5l] | 6.0r 0 7.00 8.T0i0me Exygen Research, 3058 Research Drive, State College, PA 16801 | 0110 Cn20R6 : QSutaundtyiNfoy.1S1a2m1p7l3e, ReSpetorNto.:040304AR, Ext.Date:04/03/04, Amalystik.Risha Page 9 LVSaaatsmhtpolame:odLiifsit:ed: WP:eP\d:D\DaAatptara\\L0LC7CHHS0SH8HS:S0?\3\a:Mg1a0ss1ay2l0yn0nx4x\\000088 APFO.PRO\SsmpleDS\040304AR Filter APFO.7RONMGthDB\031704 FFOR paper Last modified: Tue Mar 23 10154133 2004 [7 -- JoocbtmCtoadtse: wat spr 7 onroere0n 07o0ue0000000000 ------ cen | NTaomee: 040304AR-309 veer | IL2178-1, DF=1000 0|040304AR-308 Sm (Mn, 2x2) I : | ]= 100 200 15518455 5r0ee 400 500 06-Apr-2004 22:07:( LC/MS/MS #: MRM of 1 po Cha1nn3e1>l4E23eS05-9)) so 700 sboTime || i Exygen Research, 3058 Research Drive, State College, PA 16801 ; uti [Hetil [[| gguumaesnesritsy usammplee ReRevperotsse ou, mes pater0a/03/04, Aaslystix Risha sage 10 [ma PHoaond,tmsitstto eseddsH7BW o:s \R enDR a55SAT0T \.sWL0raaCdesassHiL2Eyyha0mNs0\\S0000T88 APFO.PRO\SazpeDEAO4G304AR Filter ADFO.SROVSHDBAGSAT0A PRON paper [oem EEEAEE prints: Wed Apr 07 08104100 2008 [--=-- [ 1: PFOA -- 2178-2, DF=1000 040304AR-3S1m0 (Mn, 2x2) . | ]1004 [| # ai5s.s13s_ | Tam a ahh 06-Apr-2004 22:23:5! LC/MS/MS ospeso) "MRMof 1 Channel ES413 > 369| eb 7b 8bTime l | Exygen Research, 3058 Research Drive, State College, PA 16801 $ Jii2 CRRR8 SQtuuadnytiNfGy.s1a2m1p7l5e, RSeoptorNto.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha sage 11 TSaamspklmeodGiifsite: d: Method: WPPee:d\\DDAaaptt:aa\\L0L7CCHMS0SN8MS:S70?\2\H:Ma1as0ssa12ly0ya0nx4x\\000088 APFO.PRO\SampleD\040304AR Filter APFO.PRO\MGthDS\031704 PROA paper ~--_oLahstcamdoed.ified: Tue Mar 23 --10:54:32 2S0E04 SE | rincea: Wed Apr 07 08:04:00 2004 | NTaemxet:: 040304AR-311 yen Le | L2178-3, DF=1000 - 040304AR311 Sm (Mn, 2:2) :. 1| [ # | - Too 200 | 300 sx pice 06-Apr-2L0C0/4M2S2/:M40S:31 MRMof 1 Chanel ES- 19752 1.A6r9e0a5 | 400 - 500 er600r|r70r0ere800Time | Exygen Research, 3058 Reseazch Drive, State College, PA 16801 | | . Jiid C0689 | uysheTrasaermee, seoapterWto.1040304AR, Zxt.Date:04/03/04, AnalystiK.Risha rage 12 | NLSaatmhpolEaes Lis.t: Bp eP:o\DaAtaT\L\e CHRSHSeT\NMaassEas1lNyymmxxS\\00?0088 APFO.7RO\SampleDE\040304AR Filter APFO.PRO\NELEDB\031704 PFOA paper [Vee rt modiR eied: Tua Mar 33 10054137 2004 Prtatea: Wed Apr 07 08:04:00 2004 | psermeet: osor0anm-322 [ame L2178-4, DF=1000 | 040304AR-312 Sm (Mn, 2x2) - 100 [ = = | i 100 2.00 3.00 T5517468 4.00 500 TY 06-Apr-2004 22:57:1 LCIMS/MS #7 MRM of 1 Cha4nne1">l63E663S69-5) | Area, rere Tine 6.00 7.00 8.00 Bxygen Research, 3058 Reseach Drive, State College, PA 16801 } | cn0G90Y i 14 [ Qauyanetif1y2S0a7mp8le.Re"5p0o8rMto.1040304AR, Ext.Date:04/03/04, Analyst:K.Risha Page 13 Semple nist: Pr\Data\LCHSNS\Masslynx\008 APFO.PRO\SampleDB\O40304AR Filter paper | LMTeaetsshttodmm:ooddiiffiieedd:: WFTeeosd\bMAApaTrra\03L73CH01E80S::T05\24H::a13g02s122y00m00e44\0D8 Ee APFO.PRO\NeCADB\031704 PFOA ---- | oTmoee:s osososn-3zs [ag L2178-5, DF=100 oo 04f0y3044R 313 Sm (Mn, 2:2) ; | ! | Tho soo - abo 13521535 4b - 500 06-Apr-2004 23:13:41 MRM of1LCCIhManS1n/3eMl>SE3S6-9 1.r1e4a68l so 70r0er 800Time | |- | | Exygon Research, 3058 Research Drive, State College, PA 16801 : us noc0z QSiuaatnytiWfoy. s1a1m2p37l0e, ReSaptorNto.:040304AR, Ext.Date:04/03/04, AnalystiX.Risha Page 14 | Mecutmhpoldme:ensisst:in sDePerab\aDhtaeetra\\L6LCCHHEoSNnHSSi?\o\MnMaasgssss1lhyyinnaXx\\00008AAPPOFO..PPRROO\\NSeaEmhpDlBe\D0B3\1074004304PARROMFiltex paper _dLoabsctomdoedirfied:oTuenMer 33 1015413T 7 20R 04 -- | NTaemxet:: 040304AR-314 [ir .ee0A=0 06-Apr-2004 23:30:2 |040304AR 314 Sm (Mn, 2x2) [- f|w si5e1z3r MRM o1fLCC/hMa4nSn/ep1M>lrS3Eo30S#9-1d) Areal || IL|| T . m 3h a- wake eh ro a-wTi; me Bxygen Reseach, 3058 Research Drive, State College, PA 16801 | . ute cnonaz SQueaanytifnoy. s1a7m3p7l8e, ReSpeotrMto.:040304AR, Ext.Date:04/03/04, AmalystiX.Risha Page 15 [7 ~ | aSasmtplemodLiisfti:ed: ethos: wPDe:e\\dDDaAtpaar\rL0C7aHS0\M8S:L?0\2MCN:aa1sHs0saSl21y0yNn0nx4XS\\00T0088 APFO.7RO\SampleDE\040304AR Filter APFO.PRO\NOLHDB\O31704 PROM paper EL> ast moFdeified: Tue Nar 23 10:54:37 2004 EE SE | parmeoyr osos0aan-sas | F[E tre oreio00 - 4oy030341A5SRm (Vn, 2:2) - |% | Too zoo ----06-- -Apr-2004 23:47:0 MRMofL1CC/hManSn/eMlSES#i- 359.51139 4133>4`83r60e95a) abo | abo 5A 00 eo 700 800Time | i | _ Bxygen Research, 3058 Research Drive, State College, PA 16801 EY] n0na3 QStuaadnytiNfey.:sLa2m1p7l8e, ReSpeotrtNo.:040304AR, Ext.Date:04/03/04, AnalystiK.Risha rage 16 Sample List: P:\Data\LCMSNS7\Nasslynx\008 APFO.PRO\SapleDB\040304AR Filter paper | MLLeaatsshttodmm:ooddiiffiieedd:: WT7+eu\deDaAMtpaarr\L02C73HS01N80S::705\24H::a1302ssl22y00n00x44\008 APFO.PRO\MethDB\031704 PFOA obcoder TeeMe Bm BET -- Text: 1: pron | Xeodos04-4, 28 ngit standard 040304AR-316 Sm (Mn, 262) _ 07-Apr-2L0C0I4M0S0I:M03S: 513 MRM of 1 Cha4n1n3el>E3S6-9 ads 4.`4A5r0e4a) | * | o 1.00 200 300 400 7 5.00 6.00 700 re 8.0T0ime | 1 i l 1 Exygen Research, 3058 Research Drive, State College, PA 16801 , ul18 creel qSutaundtyiNtoy.:s1a2m1p7l8e, ReSeptortNo.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha Page 17 = sample List: P:\Data\LCMSNS7\Masslynx\008 APFO.PRO\GanpleDB\040304AR Filter paper | _ HLLoaatsshttodmm:ooddiiffiieedd:: 7TWu1ee\dDMaAatprar\L20C37HS10N08S::750\42M::a3120ss122y00n00x44\008 APFO.PRO\NethDB\031704 PFOA a -- | TNeaxmte:: 040304AR-317 7 1 wren Co | ; L2178-9, DF=10000 [I |040304AR317 Sm (Mn, 2:2) -i |* || 10e0rr 200 300 513 S011 4.00 T 500 07-Apr-2004 00:20:2: LCIMSIMS MRM of 1 Cha4n1ne3l>E3S6-9) 8.A4r7e0a4l 600 7.00 8.T00ime t | ' Bxygen Research, 3058 Research Drive, State College, PA 16801 ' aris CO2RaA% QCuayntiotrySseaamprlse,ReSphoertNo.1040304AR, Ext.Date:04/03/04, Analyst:k.Risha Page 10 |- NolSLaaatssmhttpoldmme:ooddLiiiffsiit:eedd:: WFTeeuPe:d\\DAMDapatrrea\k02L73CaM01S\80N::SL05724\M:C:Ma13ag0H2sssE122l00yyN00nn44xxS\\00T0088 APFO.PRO\SampleDB\040304AR Filter APFO.PRO\NeLADE\031704 PFOA paper oncoger Te TB RE T -- -- | mpermeey: osososnn-sze yor | L2178-10, DF=10000 040304AR-3S1m8 (Mn, 252) .a { l |* | To.o zoo | sb 07-Apr-2004 ras MRM ofL1CC/hManSn/eMlSES#i- l 251.21638 4132.>3"0A30r659e))a abo 500 ope6r 0 | 700 800Time t Sxygen Research, 3058 Research Drive, State College, PA 16801 1 $120 "2096 | SgtuudymNsG..s1e2m3p7r8e, eSreerctNo.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha rage 19 | LaamsptlemodLiifsite: d: Method: WTePe:d\\DADaptraa\L0L7CaH0S\8H:SL072\K:CMa1ag0Hsss1S2ly0y0N0nx4x\S\0007088 APFO.PRO\SampleDB\040304AR Filter APFO.PRO\MOERDS\031704 PROA paper [SARasEtmmboidtiifieedd,:TTuueeMarNar 23 10:5M4:e32r20t04 | eTemxst:: oaosoann-ais | new . 2178-11, DF=10000 i040304AR319 Sm (Mn, 2:2) | el ; ] | | | Too - 00|- 3b 07-Apr-2004 00:53:51 513 MRM LofC1/CMhaS4n/1n3eMl>SE3S6-9 17884 1.595| Areal apo | 00 r 600 m 700 s800Time - | - i Exygen Research, 3058 Research Drive, State College, PA 16801 0121 Pte: val || quancigIy sIampEle RMepeo.rtcoto304nn, xt.Date:08/03/04, AnalystiK.Risha Page 20 YoaamntpehlodeSsohLiitsite: d: BW7eo:d\nDnapatre\n0U7CSH0S8?H1S07H3\1aM1as0ssA2l0yy0n4xe\000088 APFO.PRO\SsspleDo\040304AR Filter APFO.PRONMSEADR\O31708 PFOA paper IHeiteRaae tiodes mTueo YWearrm25a10154133 20O04S | TNaomem: 040304AR-320 [oo Ph pik . 1: PFOA SE | L2178-12, DF=10000 07-Apr-2004 ris 040304AR-320 Sm (Mn, 2x2) MRM ofL1CC/hManSn/eMlSES- #7| | | || = 11493 rea 1.075| - ~ Time 1.00 200 3.00 400 500 6.00 7.00 8.00 i Exygen Ressazch, 3058 Research Drive, State College, TA 16801 i .oul 5 T0508 ey oy a-- re. So Ho. 1040304AR, Bxt.Date:08/03/04, AnalystiE.Risha Page 21 | YsBeatmnpEolde:LGist.: d PB:\aDaete\\ LCe HBSuHSs?\HNaassssiLyymaxe\\00G088 APFO.PRO\SAEpLeDE\0A0304AR Filter APFO.PRO\HLEDB\OI17048 PFOA paper = Hoek moat ted: Tue Nar 35 10:34:33 2004 | Nome: 040304AR-321 i:e0A | 1217813,DF=10000 040304AR.321 Sm (Wn, 232) | 10( 07-Apr-2004 01:27:1. y MRM ofL1CCIhMSa/mMSES#- E51o3 4137>6r36e39a| | Tho 3b abo ab | soo 00 + 700 | 8=00Time. | 1 Exygen Research, 3058 Research Drive, State College, PA 16801 { ui23 72599 QSuiaunatyiNfoy.1sLa3mip7lse, ReSpaorrtNo.:040304AR, Ext.Date:04/03/04, AmalystiK.Risha Page 22 | ESaamplpeeeLsitsti:car Mathod: 7pPei\t\DDeartaa\t0L7CaMoS\HvSLeT\HrCNaassHsssS1slyiyNmnnxdx\\S0000T88 APFO.7RO\SampleDB\040304AR Filter APFO.PRO\HetHDB\031704 PRON paper --vLeasbt mcodoifdiede: rTu--s --Mer>33C 101540 133 2008 | mTeemxet:s oso0nm-azz r1e: eprorn | [L2178-14, DF=10000 | 04p 0304AR322 Sm (Mn, 232) - [= To zoo -- a5r1e3s --07M-RAMpro-f2L10C0CI4hMa0nS1nI:eM4l3SE:S5-| 137>.r573e609a5)) soo ab. o| 500 600 700 800Time | i Bxygen Research, 3058 Research Drive, State College, PA 16801 ! 0124 72700 F BE ER o. .0MI0MAR, Ext. Dater04/03/04, FoalyetsX.Risha pein 22 | EsaarmpEle RListE: E E P:\DataR \LCHpSaHR sSE ?\WaasssslLyymox\\000088 APFO.PRO\SanpleDE\O40I0ANR Filter APPO.PRO\NAEHDBNG31704 BPR paper fFryy I Br silver -- | mr wet 0x 07 smsomso0 300 I= ------ I _ |3 |L2178-15, DF=10000 040304AR 525 Sm (4, 2:2) 07M-RAMpro-f2L0C0C/4hMa0nS2n/:e0Ml0S:E3S#8. | h100- s5t.a1d3 413w>ar36e9s) | o Area |- 100 200 y 300 | 400 500 5.00 7.00 Time 8.00 | Spoon Sesaszah, 2058 Tesessch Drive, Stave Goliege. PA 16501 | 0128 8av0a QSutaundtyifNoy.iSLa3m1p7l8e, RSeeptorNto.:040304AR, Ext.Date:04/03/04, AnalystiX.Risha Page 24 | SaarmtplmeodLtitsitc: as Hetnoa: mDoPe\:dD\aDhvaoatr\aL\oCLrCUMEoSNoMSiSe7\\zMMiaaissdssalluyynnnexx\\000088 APFO.PRO\SampleDB\O40304AR Filter APFO.PRONHOEHDB\031704 POM paper L last moL dified: Tue-- Mar 23 10:54:32-- 2004 ----eeee-- | Taemmee:: osos0aan-aze v0 eo | XC040504-2, 50 ng/L standard 07-Apr-2004 02:17:18 | 0h40304AR-324 Sm (Mn. 22) MRMofL1CC/hManSn/eMlSES- 5.13 413> 369) - 7o04 6.9r4e0a4)) || |} 10- 0 200 30; 0 a0 eesoh o e 60 r 700 e800Time I \ | Bxygen Research, 3058 Reseach Drive, State College, PA 16801 } caver viz qSutaundtyifNoy.iSLa3s1p7l8e, ReSpetorNto.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha rage 25 - NSTaaatmthpoldmesodLiifsit:ed: PWP:e1\d\DDaAatptraa\\L0LC7CMHS0SM8US:S70\\2MM:aa1ss0ssll2yy0nn0xx4\\000088 APFO.PRO\SampleDB\040304AR Filter APFO.PRO\NOtHDE\031704 PFOA paper i Last modified: Tue Mar 23 10:54:32 20-- 04 | NToamxae:s 040304AR-325 lo] ewer | SE 1XC040504-3, 100 ng/L standard | 0h40304AR-325 Sm (Mn. 2:2) 15414378 | | ow of LL" T0200 . 300 | 400 | soo 07-Apr-2004 02:34:03 LC/MS/MS # MRM of 1 Cha4n1n3e1l>.3E73S66-59)| `Areal bo errr700 800Time |1 | | 1 Bxygen Research, 3058 Reseazch Drive, State College, PA 16801 . \ cro #hh27 [Re---- QSutaunatyiMfoy. siaLmT1p7l8e, ReSpaorrtNo.:040304AR, Ext.Date:04/03/04, AnalystiK.Risha Page 26 Sample List: P:\Data\LCHSHST\Masslynx\008 APFO.PRO\SampleDB\O40304AR Filter paper | LMLaaatsshttoamm:ooddiiffiieedd:: WTPeeu\de DAMpaarr t0273a01\80::L0524HC::a13sM02sS122y00Hm0044xS\0T08APO. PRO\HOEHDBAO31704 PFOA BIE meimener ot pr 07 aneaenc0 208 pret [ Tone == | 0| 4p 0304AR 326 Sm (Mn 2:2) [|= 07-Apr-2L0C0I4MS0I2M:S50#:471 MRM o1f Channel ES.| e561647 36.>0A0r36e65a9,,) |L Too 2b abo abo soo 600 700 bToime |i i Bergen Researchhn., 2058Research Drive,Drive, stState CollLaegeg,e.PPA 1 16801 y U12. 8 Cna70q QSutaundtyiMfoy.is5a7m1p7l8e,ReSpaorrNto.:040304AR, Bxt.Date:04/03/04, Amalyst:X.Risha rage 27 I ~ HSaatmhpolde:List: PrP\:\DDA aata\LLCaHS\NSLT\HCNaaHssssS1lyyNanxxS\\007008S APFO.PRO\SampleDB\040304AR Filter APFO.PRO\HOEHDB\G31704 PFOA paper T Tast modified: TuR e Mar 23 10:54:32 2004 ee _ | NTaemxet:: 040304AR-327 |-Tonewe[n2178:47, F=i0000 040304AR-327 Sm (Mn, 2x2) |. 1004i1 |% 232381 07-Apr-2004 03:07:31 LCIMSIMS MRM of 1 Channel ES- 4132.>1A53r06e59a) ~ 1.00 r 2.00 3.,00 4.00 5.00 6.00 E 7.00E8.00 | | | Exygen Research, 3058 Research Drive, State College, PA 16801 0129 1 chavs QSutaundtyifNoy.:SLa3m1p7l8e, ReSpaotrNto.:040304AR, Bxt.Date:04/03/04, Analyst:K.Risha Page 28 LSaasmtplmeodLiifsti:ed: Method: WPe+d\DAaptra\0L7CM0S8K:S072\:M1a0ss2l0y0n4x\008 Pe\Data\LCMaHssSLNynSx\700\8 APFO.PRO\SampleDB\040304AR Filter APFO.PRO\NethDB\031704 PFOA paper oLabstComdodeisfied: Tue Mar 23 10:54:32 2004 ------------------------------ | Taemxotr: osos0ann-sae 1: pron FE | [L2178-18, DF=10000 - 1040304AR-328 Sm (Mn, 2:2) | Lo 10 l=| wi i 1.00 2.00 07-Apr-2004 03:24: MRM ofL1CC/hManSn/eMlSES#- 21531732 4131>.953e6s9|) Areal 300| 400 500re60p0 r 7.--00re----8.0T0ime | I | | 1 Exygen Research, 3058 Research Drive, State College, PA 16801 \ 50 yrs C2706 grant seme meee rn 50 LE mies | "SamLpaslt emodLiifsti:ed: Yet 0 LTroScan WPe:d\DAaptra\0L7CH0S8H:S072\:M1a0ss2l0y0n4x\008 ro sro 2704 APFO.PRO\SampleDB\04030PTR4AR Filter paper [soomne assomacens Ley aes riptsec o | 1: PFOA 1L2178-19, DF=10000 -- 07-Apr-2004 03:41:0: LC/MS/MS #7 040304AR-329 Sm (Mn, 2x2) MRMof1 Channel ES- [ Area | TEE Se aw ewe 7s errr Time 1 | , : rg esse, 3450 Bsasee orien Sate Cote, BA 3683 my CY? QSutaundytiMtyo.sia%m3p7l0e, ReSperortNo.:040304AR, BXt.Date:04/03/04, AnalystiX.Risha rage 20 TSaasmkplmeodLiifsit:ed: Nathod PWrie\d\DDaAatpLara\\LL0C7CHHSS0HM8SS:??0\\2MH:aa1sg0ssl12yy0nn0x4x\\0000d8 APFO.PRO\SampleDS\040304AR Filter APFO.PRO\HGEHDS\031704 PFOA paper GTaeskemlodified: TTueaar Mar 73E10l:54o:3i7 2s004a -- [-- | TNeaxmte:: 040304AR-330 | nize17er:20, DF=ioo00 040304AR-330 Sm (Mn, 262) lf ey i [4 i . TTTTie0 200 OT-Apr2L0C0I4MBS3/MSST4#T 513 MRM of 1 Cha4n1ne3l>E3S6-9)| 13185 1.a2r3e0a5)] 500 | 400 | 500 | p 600 r7e 00 p800Time Bxygen Research, 3058 Research Drive, State College, PA 16801 | 0132 "2708 qSUuSeAnEeLitSy sOepEta seTpUorstucsounn, Daneson/u3/o6, Anaiyane.pisha et Co -SLfSmaarnmpleTaNeodLiiesc,Eti:an:wRNePYd:\wRDoarta0\1LS W CMSMS7S \B MasBslR OyI nLx\008 enowassom esos pron APFO.PRO\SampleDB\040304AR Filter paper | ortmets wat 20x 07 onsaasan 200s I p-- Le T-- RON ------------------------------ | L2178-21, DF=10000 07-Apr-2004 easy - LC/MS/MS #7 |040304AR-331 Sm (Mn, 2x2) MRM of1 Channel ES- Lo se pr 84170 7.58e5| [||=ot i : -we|| LL 100 200 3.00 400 5.00 600 700 800 | 1 Exygen Research, 3058 Research Drive, State College, PA 16801 1 13 vids CnR709 QBuaEntiEtyrSaempl.e ReophorNto.1040304AR, Ext.Date:04/03/04, Analyst:K.Risha sage 32 | VBaamtpehloeds: Litst:e mBePe:D\eiDRAEneA\\eLLHCEHcNSSMrSTo?\\rMMaaegses1slytynonrAx\\00008S APFO.PRO\SampleDB\O40304AR Filter APFO.PRONHOCHDB\031704 PEON paper | Memtmeeaeified: Tue Mar 23 1015~4132~20~08~ -- [| primar wed ar 07 ansoason 00s | TNaaxmees: 040304AR-332 |Co on31e7e8-e2n2, F-10000 | 0"40304AR-332 Sm (Mn, 222) 07-Apr-2L0C0I4M0SI3MTS18#7) MRM of 1 Channel ES- a571o380 4133>.5306695) Areal [= - i IL 1.00 2.00 300 "00 Sho 600 7.00 800Time || I | . \ Exygen Research, 3058 Research Drive, State College. PA 16801 vid: 4 "0710 QStuuadnytiNfoy.:S1a2m1p7l8e, ReSaptortNo.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha age 33 [eSLeaasmtpelmeodLiifsti:ed: WPe:d\DAaptra\0L7CM0S8eM:Sr072C\:uM1aa0ssssi2lyy0an0sxh\c0e0n8 A0P7F0O..PsRROo\\SKaemtpalRe\D0B3\1074004304PArRonFilter paper = Laosttmmaodritfied: Tuoe Mear 23 10:54:32E2004h -- --_-- | NTaemxet:: 040304AR-333 | xeceoorsois,250 ng/L standard 040304AR-333 Sm (Mn, 2x2) | 100 07-Apr-2004 04:47:5: LC/MS/MS 34571229 MRM of 1 Cha4n1ne3l>E3S6-9) 3.08e5| Area 100 2.00 r 3.00 400 50+0 6.00 7.00 8.0T0ime | 1 | | | l 1 Exygen Research, 3058 Research Drive, State College, PA 16801 1 , 0135 n2va J nay hoTi-- ara, SatNo. 1040304AR, Ext.Date:04/03/04, Analyst:K.Risha Page 34 || SEaasmtplmeodLiirsit:ea: ether wFeePdro\aDaEapetraL\0L7ECHSoSnHT:SoTza\:Kisa0sysl2y0m0n8s\000088AAPPOO.. PPRROON\HSOALEApDLEeND0B3\1O740O4INPAFROAFilter paper oYr ert mc oatca ied: Tue Mar 35 1015413 2004 [| peimuas od apr 67 anusasoo 200s | NTaomret:s 040304AR-334 | v[e217ns23 bF=i00 [ - 040304AR334 Sm (Mn, 2:2) | "* 07-Apr-2L0C0I4M0S5I:M0S4:#376 siososy MRM of 1 Chamnnesl E,S- 42103 rea o Ti zoo abo abo | spo sb 780 boTime bt i | | | | i ` ayes Rucoaehe BIE Ripancch Drbve, Fave GoRAgR: THUR 1 013s crn | gQuuanaenietyisfyaSmapmplleeReBOpIoLrit ps0in, Exe.Date:0d/03/04, Aaslyst:k.Risha Page 35 |- fnLESraaassOmeetplnimeoedLiiisfeti:aed:: WT TPhe:uetd\eDvAwaepatrar\L037C5HS0dH80S:ir 70s\2kM:o1an0ssIole22oy00nn0sx4\000068 APPrFoO..EPRROO\\HSeasmApDlEeND0B2\1074008304PATRCAFilter paper I [i---- 1. pron | L2178-24, DF=100 - [ ] 014000304AR-335 Sm (Mn, |* J 07-Apr-2004 05:21:1 LC/MS/MS 2x2) 55610348 MRMof 1 pol Cha4n1ne35l>.34E36S0-95 | 1.00 2.00 3.-00 4.00 T 500 6.00 7.00 - 8.T00ime \ | . \ .. Exygen Research, 3058 Research Drive, State College, PA 16801 u1nn372o73 QCuaanytintoy. sa2m0p7l0e,Re"SpaotrNto.1040304AR, Ext.Date:04/03/04, Analyst:K.Risha Page 36 NTSeaatsmhkpolameodLiitsit:eds WTeeP:d\\DDaaaEpteaa\\L0LC7CHHE0SN8HS:ST0?\2\M:Ma1as0sss12l70ya0nx4x\\000088 APFO.FRO\SampleDS\040304AR Filter APFO.PRO\HSEHDE\OI1704 PFOA paper | oaosntcomoddiefried: Tue e Mar 2m 3 1054132 2008 [| seimcuar wd rr 07 oneassan 200s | Nparmeed: 040304AR-336 Lo| : v[2e178.25 DF=100 1040304AR-336 Sm (Mn, 22) - | % oe me, 07-Apr-2004 05:37:51] MRM ofL1CCIhManSnIeMlSES#-7] aes 45r4e0a5l Too zoo 500 abo 500 | ebo 700 800Time 1 | 1 1 | Exygen Research, 3058 Ressarch Drive, State College, PA 16801 \ . 0138 cana QSutaundtyitNoy.1S1a3m1p7l5e, ReSpetorNto.:040304AR, Ext.Date:04/03/04, Analyst:X.Risha rage 37 | iSaamepklmeesniiisitc:as Hothod: ePCPa:\t\DDaeatrtaa\\L7LCCNHSoSHnHSeST?s\\HiMaaossss1alyyonntxx\\00008 APFO.PRO\SampleDR\040304AR Filter APFO.PRO\NGLRDB\031704 PFOA paper So _ onlatset smoedified: Tuues aMarr |23 10:54:32 2004 EE - a | eTemxetrs oaor0anm237 Tamron SE | [z17e26, DF=i00 [f0| w40304AR 337 Sm (Mn, 262) - |% 3TT 200 07-Apr-2L0C0I4M0S5I:5M4S:2#4 465332 MRM of 1 ChaTnneliES4.4105 Area a00 rs400 | soo | 60 700 800Time| 1 \ 0 ' 0 Exygen Research, 2058 Research Drive, State College, PA 16801 ' . . 0139 CYAS Qe uantitye sampte ReeptorNto. 1040304aR, Bxt.Date:04/03/04, AnalystiK.Risha rage 30 [ NocE ukmnpolde:i List: 7F:o\nDarttear\LLCHCSoHSHnT\eHNgaassgsMelayynamS:xr\\00T0088 APFO.PRO\SspLeD3\040304AR Filter APO. PRO\NEDB\03ATO4 POA paper | i Mertmsateied: TTeue iwaer %35 10:54:32 2000s08 BE I | recess wea rox 07 onsons0 200 | NToanmees: 040304AR-338 | miezisreena7 oF=100 07-Apr-2L0e0m4s06i:m1s1:0 - 040304AR338 Sm (Mn, 2:2) | poinh ssaire MRM of | Cha1nn3ea>ltEe3S6s-9)) Area |% | > Th abo abo a.bo soo soo 760 re scoTime | || i \ Exygen Research, 3058 Ressazch Drive, State College, 7A 16801 | 0140 crave QSutaundtyiNtyo.siaLm3p7l8e, ReSpeotrNto.:040304AR, Ext.Date:04/03/04, AnalystiX.Risha Page 39 | LMrSaaatsbmhtpoldmmesoedLiidfsiti:ede: eTPPe:ud\e\DhaMDtaeara\Lt2oC3rMaS1oM\n0Si:7oL5n\4HM:Cda3as2sUsslS12ayy0onHm0dx4x\S\0007088 APFO.PRO\SampleDB\040304AR Filter APFO.PRO\HSEHDBA031704 PFOA paper [s--oevicnoedner-- --wea noe 07 oni04O s00 20O 0s | pNaemset: 040304AR-339 Leen | L2178-28, DF=1000 [A040304AR-339 Sm (Mn, 2x2) { | |* . 100 2.00 su23876 pant 3.00 400 5.00 TS 07-Apr-2L0C0I4M0S6/:M27S:# MRM of 1 Channel ES2.A1r1e6a5 Time 6.00 7.00 800 i Bxygen Research, 3058 Reseach Drive, State College, PA 16801 | C0141 cna? | | quancaty sampe separ Study Ne.:L2178, Set No.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha rage 40 Sample List: B:\Date\LCHOHS?\Masslymx\008 APFO.SRO\smLeDB\O4DI0ANR Filter paper | eHLFaae tsthtosmh:oadaitfeieedd:: uWB eod AHparro S03By57u0s180y:in 0o24:+e13a05s22ve 00a00t40\0e0e8ereAePmFmOe.eBeRmOm\eNeArErEDBG3170e8 aPIOmN es | Teoe comasmncseo 1: pron | |L2178-29, DF=1000 040304AR 340 $m (vn, 222) | p . | * _ 07-Apr-2004 06:44:27 MRM LofC1/CMhaSn/neMlS#57. E51o3 413T>oer3o6a9s) 1| 0- Tih zTh ak aT bn sh7 o eho zoT o 5%Time i 1 I 1 1 w I 1 | Sirgen Research, 3058 Research Drive, State College, FA 16001 | ui42 cons qSutaundtyiNfoy.:SLa3m1p7l8e, ReSeptortNo.:040304AR, Ext.Date:04/03/04, Analyst:K.Risha Page 41 | ESaasmtplmeosLiirsti:eas Pw:e\dDaatpar\L0C7MSoNsSs7o\zM:aiossl2y0n0x4\008 APFO.PRO\SampleDB\040304AR Filter paper MLeatshtodm:odified: PT:u\eDaMtaar\L2C3HS1K0S:75\4H:a32ssl2y0n0x4\008 APFO.PRO\NethDB\031704 POA | --obrracceesmer--wea nor 07 assonsen 2000 | Teemxetr: osor0anm-3az | |XC040504-5, 500 ngiL standard | |0140003,04AR341 Sm (Mn, 2x2) - |1 i % 07-Apr-2004 07:01: LC/MS/MS # 511 MRM of 1 Cha4n1ne3l>E3S6-9 69196 6.5A6ree5a, || Io "100 T 200 30T 0 4.00 500 6.0T 0 7.00 80T0ime t 1 1 i | | | | Exygen Research, 3058 Research Drive, State College, PA 16801 e ui4d "719  QSutaudnytifNoy.1sLa2m1p7l3e, ReSpeotrtNo.:040304AR, Ex.Date:04/03/04, Amalyst:X.Risha Page 42 [ELSeaasmtpelmeodLiifsit:ed: WePdi\DAEaPrtEaS\0L7CH0pS8N1S0?3hi\1Ma1ag0sases2l0y0n4xs\00008s APFO.PRO\SampleDB\O40304AR Filter ao.rro\weEhom 31704 pron paper -_gLabstcmoodiafieed:.Tue Mar 23 10154133 2004 | NToemxet:: 040304AR-342 TM Rte soeeee | [XC040504-6, 1000 ng/L standard es 040304AR-342 Sm (Mn, 2x2) - | 1i0) | 100, |[|= To ah _ i . . ha: h eet eee SSM------ETETTIEE 07-Apr-2L0C0/4M0S7/:1M7S:5#: MRM o1f Channel ES- 1450.31425 4131>.v223e66a69l)| . she she 7os absTime | " | - i Bxygen Research, 3058 Research Drive, State College, PA 16801 ui4d c~2720 | ViFrecohveee RnesRarecsn hs . l- The following pages are exact copies of information for the standards used in ths study. |~ P2e3n1t1a6dHecBa)flwuaosrouoscetdantooimcaakceida(Isntovcekntsoorlyut#i:oSnP(3I8D00#),$0o3b0t2a0i4n-ed1)fwrohmicAhldirnitcuhr(,lowta#s used to make intermediate solutions (ID#'s FO30804-1, F030804-2, F030804-3, F030804-4 - astnadndFa0r3d0s8,04l-a5b)o.ratTorhyesceontirnotlersmaemdpilaetse,soalnudtimoantsriaxresupsikeed staompprleeps.are extracted calibration | PInecn.t,aFdleucoafrloucohreomocUtaSnAoi(cloatc#id2(1I0n0v0e2nt)owrays#: uSsPe2d6t5o1m),akoebtaaisnteodckfrsoolmutOiaokn w(oIoDd# PSr1o0d2u7c0t3s- 1) which in tum, was used to make intermediate solutions (ID#'s F012804-1 and | Fis01d2e8s0i4g-n2e)d.asTahnesiendienpteenrdmeendtiastoeusrocleutvieornifsiacraetiuosnoefdtthoeprceaplaibrreatthieoncshteacnkdasrtdasn.dard, which | Tinhfeorimnaftoiromna,teixopniorantithoen fdaotlelsowainndgsptaogreasgeprcoovniddietisotnhsoefextahcet nseoautrcsetsa,ndlaortdns.umbAelrsso,isnhcilpupdiendg | tahree tehxeacptredpeatarialtsioofntohfestporcepkasrtaatnidoanordfaenadchfoorfttihfeicastitooncksoalnudtifoonrtfiofrimcsat.ioTnhseosluetpioangse,sasshwoewll as their expiration dates and storage conditions. - 3 AAS - | 5 VIELE Bln usa utes rn, 4 CRA EL ge | &% |e3 I5 || g82 5oF Ss 8 Ge - 58 | . Z-- s g 2 S2e8. 2 B 2 S288EpEsEEE%eBudv a 1 gs, EE lc iacieo53 i 583 i3i zg HgiiL:I Ie:: 253 . ii 1 3 Yo. 12 gg 8 iE ; -- E 3 g i. | 4 thi i id i 1 : z 1 `P--=: 3 re 83 i: il i 22 3 3ist s 3k Wp Y-i-i 8-38 1 sss SS E cL 32e 6Sefoctu t5i5a.33 3 3tR8gsidg 2Billy mussmencroorvor i THEgGNALOTi E, | 8 & BSsEe w ! iClad Wl BSCiisasis3be.ai8pt.e.gcsiapi3lgfiiffvn5riis1iii5s2fits.d 7WIBEEE come 0 OT4S 3. ll | : # 4 |: 3 . |p SSMAALDRCH 5 2 agigbedatfgpetee } AllentonDistribution Center if i Aldrich Chenical Cony Inc, 3 ~AttentounPA T6108 El r|}. SEHIxPoTOn: neszanch Hi .P ETi I Co "i7. 3B0T4A8T.ERECSOELALRECGHE DPRA 16801 i. Ad - Tole dm Cok T = ] o : [ Ii y .id3 SE on selmi Sl een ai] [ERI A lee| | N I ETaRINA J00LEPERTILE 0CONTA"C jSh ateSsHeoan0AEEAeasre FLA onote Bo pore tee Yea1 RE-E oa [BNan TE[EETITree] O [PENTADECACFIDRELoSeCUIATOICONIRADO,9O8C7E_TRASNRD. OIC| |[einBE EE ea IS oepn lialin: a | p[sr noersunsiwsazsauwa EPEIENLxerie oma nanS-- ms a a0 RRR e EY|S a eNERnale oun PRES oy SRE Hes REE ARR EE HErSeBAre ] [Jasons dursnaad GE me ASB A SA Sam [FEIN soz Hes v0 elvannerd RE mers |Fownsic svfry sven le | DE 8S REET TEEaRSEi| son P-- mS ie SRE ER ro em MRS ERA a [STahCeMAMDRCH fBsRohEmmEa HSFmElGueEkeaLh RQfEAmt,R Dh-- mmmoamom, GSosuepSELoC.O | 5 TUNE LT or - | " NE Re Ce EET IS I AN EXACOPCYOTF. "THEORIGINAL DOCUMENT. orToreain . 0147 | C03 ] |i | | | | | || || | J f " | 0 so RC Ly Di 3 BN oN POMENTPPED FROM Allentown v ii ooo 3 P 0003253010 = = \ FB oovcon ese : | C-- R -- ; fi en rT i THIS PACKAGE CONFORMS TO 49 CFR 173.4 bRE ea D ie eRUD E Lhe ae || | IB FL mss anexscreorvor | 003 = BE one ie REE bl HEE Hest oe Si G3) [4 GSAT fn are ET 16801ZIFCO0E Si 12/05/2080 : IEEE TTT, {5 H|l cGovGeGGoGc"GrioGorGGGWGCGEGAGG3G0GEG | i1 1; | | HHT HT HHH HTT] EY Arreorne | Ii | I [| 1H lf rs l T il| l H1 H H| HT HHITEE BeErS r2rr 27S ma 2 4sn 6271m8 5r 44187a 4s 6w2urtsre |_ pPPrroaduutcot nName ppaenvtasdtecafiuorooctanoic acid | Molecular Weight . 414.1 | Test J-- L-- orzu ---- We To aE POWER nweeCRrsTAIE - Ian ETE CONFORMS TO STRUCTUREANDSTANDARD ET 4 LLUSTRATED _ a CaONnFORsY0 ST0 _---- S550. 108.55 CHIH AAO) nH | SASLIQUID ony 95.5% (au) 77% -- I ser 0 wim an. [ squamsesrymceonmTmeos J ZNPa 2 I| Q`uRiointayCJoMnasttri]n,Supervisor | | p | "THIS IS AN EXACT COPY OF THEORIGINALDOCUMENT? BY oare_odloTleL 0149 CIES | | | | `Protocol No.: [------ : " RESEARCH S3i0t5e8 CReoslelaergcehPDAriv1e6.801 . SP 3800 PhoFnaec: s81a4:n2712.a10m39 = Exygen Study No: __ FR TANT COMMINCATIINE Page [of | nro | Person: _Ken Company: _Aldrich | a=- Originator: ~~ Exygen __x Sponsor Date: _12/08/03 Time: _1022 Notes: Icalled Aldrich tech service at 1-800-231-8327 to ask the shelf life of the PFOA standard | Ireceived today. Ken, an Aldrich rep in technical service, toldme that the shelf life is 2 years from the date of receipt stored at room temperature. | - |{ . ! Signature 4ule) i_ . THIS 1S AN EXACT COPY OF orT THE ORIGIE NAoL DeOCUMR fENaTle, _ pate 2803 cram 0150 August 7.20001 | . [ [ [-- ) 3058 Research Drive Phone: 814-272-1039 - RESEARCHState College, PA 16801 Fax: 814-231-1580 - PREPARATION OF STOCK STANDARD -- SOLUTION on 1 T `ExygenE StudyA / Projecta Number:1 RY `CustA omer Project Number: FEES) | PHI | ___NA EATAS TdT Es FE | | Prok Primary StandarSdPExopi0ra0ti3on8D0al0e |- SS --__ ao A Net Wt. of Compound: 0.0242 9 T--_"" Net Wt Adjusted for Pu1r2y]: oo8o)ocSco i a | Final Volume: on mt "Stock Solution Concentration: oco pgm. | Siok Soon REC0O% Stock Saiaon ExplaetriponANDaDlLe: | (a SFWrUaeUeiOzneDrYFYDrio16a 2za3r.TD0:_)_~ _--=_ SOB0eZORWaaeUlf-kiiLrngoCl raoloo0r:l9. 3=3e : RSotoormagTeCeCma2p2beirna1ettu:0re:0= T_E= |- Ea ET mTetEo Roa i FEE i | T"THHEISOIRSJAGNINEAXLADCOTCCUOMPEYNOTF i BY. DATE Ot { i - | Signature: rySheeler Date: _08Jo2Jo4 | J BRARY 0: vo0009864 LABORATORY FORM |i o Jib5d 0am | 3058 ResearchDrive. - - Phone:814-272-1039 RESEARCHState College, PA 16801 Fax: 814-231-1580 |- oo PREPARATIOOFN FORTIFICATION STANDARD SOLUTIONS' - | EaEin Sy rolCeR: Gusto Projoct R Number: E PH A | ECEoETEE on Soars Sphain bat 03lo8|0 obo! | r mason | Reogooiazn)s2!|"[son| METHANOL [ATTS| | pEatTa,| F|nsEsea|n.| ormMsonRo [ [ [cotm oh p |1o0|r10s| ma wo 100 |p01|ro3teed-s |- STO7AGECondition andLOCATOR JASEEaeOpEe |- Crate Froszar 0 E=i__nbWvamleknintGofaooln irI0:---=_ prSstocroagnediCa)binet: =- EEE er | I `| > "THISIASN EXACT COPY OF oH oe lel, THEORIGINAL DOCUMENT." | Signature: (bios flabs - pate: tEploY 0152 CTO708 snovevesaz les 1 |goan ) gnE o] | &% i3 3] [| IBd 22 &gi (IeEsgE8 ) rr | zs 23 33 :8 |8 3 g3 E [ba oog-s S808i33e5sh%,0c0e8o0 gf He 83g 38 3S3e2g8s5f5e5c5e5t F8E8 g 8g5 [ So PoE: 2 3 . qFafl: C8G ,s2525285.88 28 Hy 2 [fl Sosd2siEe ME E 2 Bed = 2 z 2 0 tA 5 :g 3 pi E] | c Ez 3 g ] 3 ] i . 2o 2 | iti |- . 5 4-c4f-o4f | =5 8g & oSEfE l | = `HH3iH:E aosi_y TI8ES8 2d #8&58Z BmO :5 ]- or "TTHHIESORISIGAINNEAXLADcCOTCCUlOMPEYNlTO"F |i Rb EE | R=8 2E E |BIeA8P.ISTO5T2EA Bilassigscsiissacl uyisss g cong g [oo Sro2nanoneTe: he. | - W1EST 7 OCLO4 DLUNU1 BAMRSBCOI2A8AD17,2 Pr 803:739.800 FAX: 803-739-6057 . am5s0 erpER i | a Ew selentiric {| [T BridsepE ort, Ni 0R0%4 _ bage 1. Tsoure |ABcakyagren10Ks8401133h Labs RSetfat2e0CoLlOlTaIg0e1,1P39A T6OT i (856) 241-7908 [EEE SE ! {cero FEe]omenTwomaworasdooifim] wirbate Tone Ja|ronaenaTo|0 RTRRORNE/ TOY =F bs br {Sir bi ; 260|eg] ome r pron rans|| fencadesatluorosctanoic acid, 7% Lede my Ti aa REE {4a | Lcd I i eal + ror geno002 ouiy tes ! SEE a oH HEEL | HEE Ee Sa r- -i |- i| ) [1 - |] I -| ++ CERTIFICATS OF ANALYSIS ILI Bie } REQUIRED RGaE mE aneat | i . BEEe Teareea : rwoma i m gaanlt - ; a .wh oh ed EE - PEER . SE REee a ar (- | . ni - He : "THISIS AN EXACT COPYOF M ELREE THE ORY nooo [oll Be orEH0 ove M. aE nimw m0 .: ~ | P=ACKING LIST [ Ren 4 omen Eoin, om Dean edevoy : F a E natT al he i ibmieiesee-- i Cnevso U1s4 | | | a rE EE |! - HiL ii @ EcaEnons mses b7ia Saem ea e | | QD) mn i .a we | Fl 10: Engen researchLabs Lh L m aa y n : FihR 2A0t6t8n:RLeOsXe4a0r1c9h5D9rive | Saeco e WE e na Sedan il PA16B01 RefPO LOX401139 ERAT | a [ ie VIRINTIRER | EL | Li i CRTRy | HH i TIER ERE CL { Hl | Hm il | i i ee dens TH | (EAA ER [HHT EF EE |e smeecrcoror Fee rygen Rasearch Labs. | hives STATES /EORIGINAL DOCUMENT iy. BY. DATE { <p> Sounan | J T= sesso S TUE | | HAZ ee WCEA 3Q ABH PLT fesse postoxionm pi | J ni HH | HEHEn AIRBORreNnE rey, bm E paS se | RH EXPRESS isin og | LEE i HE S Terme teee -- WEST CO om LUM, SC29 172 weigh des On 4x00 PToogo SDiovaen | sn RETR us ny {- | aa 4 peeeseenorsemT mmE meREnTmyET eT TTT bol FR] - | EE Hi i chowgg UISS r bos - | : - . [- % O1u70k1w0ouodrProdiducItnsc,. sl esColu5mn72 r ---i i Pm uro 55 79.350e 0 ee | ~ fe i. CERTIFICATE OF ANALYSIS Dates Tab | | Melb Penidicaluoroactaancsicd [ cameo ) Li Caos sera 1 LatNo: 210002 a l Hata 973% byNaOH (ization Lo Awenme Waitesata gu4 : Meinebint: 5061C ~ I. y ro ee re ` nad Vi . | {: TT o THEY ORIGINAL elle I" | 3a [ E en 3058 Research Drive Phone: 8142272:1039 ATitasch State College, PAT6801 Fax: 814-231-1580 | Page_ i PREPARATION OFSTOCKSTANDARDSOLUTION I Protocol Number: __\[A BxygenStudy Number: D0B-APED | Compound: FENTAMCAR (Pek) UORDCCTANDICACI, I LotNumber: 200002. ; Source:CMWENRONS Purity: 47% DateLastCharacterized: s1li5|02 | Date Standard Received: _0M|0A0% StorageLocation: WAIN (00ER] [ Compound Expiration Date: _04|0%{0v Exygen Inventory #:_SP2bS A calibrationcheckmst have bn conductedwith balance on th dyofsample weighing [. `Balance Calibration `Stock Solution Balance Number: ___\ Net Wt. ofMaterial: 0.61031 _g | Logbookpage for We. Adjusted for Purity: 0.01600 calibration data:_EX{UMS = BN Solvent: METHANOL. Final Volume: bo mL | Manufacturer: EM | Lot Number:_U3238336" Concentration: 0 pg/mL "TTHHIESOIRSIAGNINEAXLADCOTCCUOMPEYNOTF ar Ge | StocEkxpSiorlauttiioonnDatee:_01}]09] od} StocSktoScokluStoinoInDI#D: SSN1M0DD2Y7Y0-~312,103. I *Assign aoneyearexpiration dateforallstocksolutionsunlessotherwiseindicated by sponsor. Storage Conditions for Solution: | . FreezerNumber: _T_--_aRdefwrhiigcehroafttohr Naubmobvera:ppe_a2l2, axRooatoomn.Temperature: _~ | REMARKS: || Signature: lo cnavaDaate: [U0125773ly 18,2008 . 3058 Research Drive. Phone: 814-272-1039 State College, PA 16801 Fax: 814-231-1580 RESEARCH PREPARATIOOFN FORTIFICATION STANDARD SOLUTIONS - Exygen Study Project Number: `Customer Project Number: ! | ortification Date Prepared: .y Pa repara ation: aForlifiaDtiIonRSolutionUsaExpi0ratiSoneDate ae oi2eley Aloo | `Solvent Exygen Inventory ID: REGU Lb `Solvent Name: METHANOL compo | Son|TTHATLSSCE | Foarson.| Fostson.| rie som | [wos[room[too| ro [eo|vo Jromeir| - ee i- T IAS A ENe EE BE CA |- | F- LT] - [Storage Condition And LoCation: {ieEsSs A Lee| . Walkin Freezer 0: _T_or--t_o_ra_WsianlamienntoCroFooleor1I0D:r= der aspreStcoroangderC) abinet: | |RemarksKis CanRAR Je AE SRE Raa) [ _ orT"THHERISOIRSEIAGINNEAXLSADCrOTCUCMOEPNYTlO?Fel I Signature: (belle i! Co Date: oiled ibd < TTR I G5eNObV)EMBEoR t2003a/4." 735