Document ybGooYgpoOYXEB7pGBQBnDdQ2

6" 3 77,,-) p. 1 -~ V ~2 ~ ACUTE TOXICITY TO THE FRESHWATER AMPHIPOD (Hyalella azteca) TEST SUBSTANC E Identity : Perfluorooctanesulfonate ; may also be referred to as PFOS or FC-95 . (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3 ) Remarks : The test substance is a white powder . Sample was taken from 3M lot number 217 . Sample was stored under ambient conditions prior to testing . Purity determined to be 86 .9% by LC/MS, 'H-HMR,99F-NM R and elemental analyses techniques . The impurities were identified as : metals (1 .45%); lower chain-length (C4-C7) perfluorinated sulfonates (8.41 %) ; inorganic fluoride (0 .59%); NMR impurities (1 .905%); perfluorooctanoate anion (0 .33%); and the total of tri-, penta-, hepta-, and nonapentafluoropropanoic acid (0.38%) . ~ METHO D Method: ASTM E729-96 Type: Static renewal GLP : Yes Date completed : Study completed 2007 Species : Hyalella azteca Supplier: Environmental Consulting and Testing (ECT), Superior, WI Analytical monitoring : PFOS measured at initiation, prior to and after renewal at 48 hours and at test termination (96-hours ) Exposure period : 96-hours Statistical methods : Bionomial probability method used to calculate the 24-hour LC50 values and probit analysis to calculate the 48, 72, and 96- c, hour LC50 values . The NOEC determined by visual interpretation of mortality and observation data . Test organism age : 12-days ol d Average wet weight at test initiation : Not measured Loading : 1 amphipod per chambe r Pretreatment : None = Feeding : Fed YCT plus Tetramin flake food at Days 0 and 2 Acclimation : Prior to the test, the test organisms were acclimated to tecl conditions for 6 days by holding them in a container with gauze and water from the same source and at approximately the same temperature that would be used in the test . Temperatures ranged from 21 .8 to 23.4 C . pH ranged from 8 .1 to 8 .5. Dissolved oxygen ranged from 6 .4 to 8 .0 mg/L . No signs of disease or stress were observed during the holding period . No information was provided on the characteristics of the "gauze" or its suitability for use in this application . Test conditions: CONTAINS NO CB 41* *'6 p. 2 Dilution water : Filtered, aerated, UV-sterilized well wate r Dilution water chemistry ( during the 4-week period immediately preceding the test) : Specific Conductance : 290 (285-295) Nmhos/cm Hardness : 136 (132-140) mg/L as CaCO3 Alkalinity : 182 (180-184) mg/L as CaCO3 pH : 8 .1 (8 .1-8 .1 ) Stock and test solution preparation : A primary stock was prepared at a nominal concentration of 200 mg active ingredient per liter using a calculated amount of PFOS, and stirred overnight . For Day 0 solutions, the stock solution was sonicated for approximately 5 to 10 minutes the following day . The Day 2 stock solution was sonicated for only 5 minutes the following day . After mixing, primary stock solution was proportionally diluted with dilution water to prepare each test concentration . All test solutions were adjusted to 100% active ingredient during preparation, based on test substance purity. All test solutions appeared clear and colorless at test initiation and termination . Solvent : non e Concentrations dosing rate : Static renewal after 48-hours Stability of the test chemical solutions : under test conditions : Stable as demonstrated by analytical monitoring . Exposure vessels : 30-mL Nalgene plastic beakers containing approximately 20 mL test solution . Water depth was 1 .9 cm . A piece of Nitex screen was placed in each vessel as a substrate for the amphipods . Number of replicates : 10 (test substance), and 20 (negative controls ) Number of amphipods per replicate : one Number of concentrations : Five plus a negative contro l Photo period : 16 hours light and 8 hours dark with a 30-minute transition in simulated natural sunlight from fluorescent bulbs . Water chemistry during the study : Dissolved oxygen range (0 - 96 hours): 8.2 - 8.5 mg/L (negative control exposure) 8.1 - 8.5 mg/L (all test substance exposures) pH range (0 - 96 hours ) 8.2 - 8.6 (negative control exposure) 8.1 - 8.6 (all test substance exposures) Test temperature range (0 - 96 hours ) 22 .4 - 23 .7C (negative control exposure) 22 .2 - 23 .8C (all test substance exposures) RESULT S Nominal concentrations : Bk control, 13, 25, 50, 100, and 200 mg/L 417 p. 3 Measured concentrations : <LOQ, 13, 25, 51, 102, and 199 mg/ L Element value: 24-hour LC50 =>161 mg/L (C .I .> 102 mg/L) 48-hour LC50 = 93 (70 - 125) mg/L 72-hour LC50 =29 (20 - 40) mg/L 96-hour LC50 = 15 (4.5 - 24) mg/L 96-hour NOEC = < 13 mg/ L All element values based on mean measured concentrations Statistical Evaluation of Mo rtality: Binomial probability with nonlinear interpolation or probit analysi s Analytical Methodology : Analyses of test solutions were performed at Wildlife International Ltd . The method consisted of dilution of the samples 1 :1, v/v with acetonitrile, followed by secondary dilution using acetonitrile : HPLC-grade bottled water (50 :50, v/v) and analysis by direct injection high performance liquid chromatography with mass spectrometric (LC/MS/MS) detection . The LOQ (limit of quantitation) was 2 .00 mg/L in this study . The measured concentrations of matrix fortifications analyzed concurrently during sample analysis were 98 .4-114% of nominal . Samples collected in exposure vessels at test initiation had measured values from 100 to 105% of nominal . New samples collected at 48 hours had measure d concentrations from 95 to 100% of nominal . Old samples collected at 48 hours had measured concentrations ranging from 96 .4 to 103% of nominal . Measured concentrations of PFOS in samples collected at test termination were 100 to 102% of nominal . Summary of analytical chemist ry data : Nominal Test Measured Values of New and Old Mea n Concentration, Test Solutions, mg/L(l) Measured Percent of mg/L Concentration, Nominal mg/ L Negative Control All < LOQ1 2 1 <LOQ - 13 13 .4, 13 .5, 12 .3, 13 .0 13 100 25 25 .1, 24.9, 24.3, 25 .4 25 100 50 52 .6, 50 .2, 49.4, 50 .8 51 102 100 102, 103, 100, 101 102 102 200 204, na , 193, na 199 99 .5 (1) Samples are listed in this order : Day 0 (new), 48-hours (old), 48-hours (new), 96-hours (old) (2) LOQ = Limit of Quantitation = 2.00 mg/L (3) Not analyzed due to 100% mortality . Value not used in statistical calculation s Biological observations after 96-hours : All but two amphipods in the negative control appeared healthy and normal throughout the exposure period with no mortalities or overt signs of toxicity observed . Two control amphipods exhibited lethargy that resulted in mortality . At test termination, mortality in the 13, 25, and 51 mg/L treatment groups was 40, 80, and 80%, respectively . Signs of toxicity observed in the affected 4(,F p. 4 amphipods from all treatment groups included lethargy . All survivin g amphipods at test termination appeared normal . Cumulative percent mo rtality : Mean Measured 24-hours 48-hours 72-hours 96-hours Test Conc. , mg/L Negative 0 0 5 10 Control 13 0 0 10 40 25 0 0 40 80 51 0 10 80 80 102 0 50 100 100 199 10 80 100 10 0 Lowest concentration causing 100% mo rtality: 102 mg/L Mortality of controls : 10% CONCLUSIONS The potassium perfluorooctanesulfonate 96-hour LC50 for Hyalella azteca was determined to be 15 mg/L . The 96-hour no mortality concentration was < 13 mg/L and no observed effects concentration was < 13 mg/L . Submitter : 3M Environmental Laboratory, Maplewood, Minnesota, 55144 DATA QUALITY Reliability: Klimisch ranking = 1 REFERENCE S This study was conducted at Wildlife International Ltd ., Easton, MD at the request of 3M . OTHER Last changed : 07/10/07