Document yXmnrjVG2ZvbEqzoVxgnNqrn

i-3 EETR226 -- 1017 :~ i i - rn CENTRAL SCIENCE LABORATORY >> i i [reid i, LY L. a i, e Ly STUDY NUMBERHTS602 PERFLUOROOCTANESULFONATE, POTASSIUM SALT(PFOS): _ TiTTHHE EHONEHONYEYBBEEEE & MAFF - 000002 CSL Study Number HT5602 STUDY NUMBER: HTS602 PAENRFALCUUOTREOOORCTAALNTEOSXUILCFIOTNYASTTEU,DPYOWTIATSHSITUHMESHAOLNTE(YPFBOS0)2: REPORT AMENDMENT NO | EPPO GUIDELINE 170 OECD GUIDELINE 213 i AUTHOR: 3 Paul Wilkins STUDY INITIATION DATE: 11Sepember2000 STUDY REPORT DATE: 23 March 2001 SUBMITTED TO: Evir3oMnCmoerpnorLaatbioornatory ScPa9u3,5 MBuiUsmshnkAevseonu5e5106 : Pertrming Laboratory: 3 EmEinvriornomnemnenttlalBRo&gDsTGeeaomnp 7 Central Science Laboratory = YorkSYanOdu1Hut1t1o7n, UK Environmental LaboratoryorFPrroect NumbPeargeU2l7of235 000003 (CSL Study Number HT5602 CENTRAL SCIENCE LABORATORY `CONFIDENTIAL Study title Test fucilty Study number Numberofpages: REPORT AMENDMENT 1 tPoexrifcliutoyrosotcutdaynwesiutlhftohneatBeo,nePyotbaesesium Salt (PFOS): An acute oral Environmental R&D Team, CSL, Sand Hutton, York, UK HTS602 5| i Signature, ithe Date OS/otft @aimmiyy) SPEntWvuiidlrykoiDninmrseencttaolr R&D Team, CSL Authorisation of release of report amendment SEE sigmue___|[Athens Management Representative MHeHadBEenvwironmental Biology Group, CSL Date_:&-01 (dd/mmiyy) eee st 2 This amendment has been noted by Quality Assurance as correction othe report 4 A sigue Cl ass (Dulles Date|C8tel(ddimmyy) MQrAsACudVitWoarlker Central Science Laboratory Environmental Laboratory Prcject Nomber U2723 Page 2015 000004 CSL Study Number HTS602 "wRTheheiasrsehoantshfeboeTreeansmtceoInrideemmctehenadtd:tboeTPehenerrfmeliuswo-arsospoeclttaaynspeosPguerlrfafopunhoairtcoeao,lcPetoartnraeossusolnifupomanSgaetas,l7P(oPatFnaOdsS)s.ioumAtfShfalotrfmi(naPatlFiOrneSgp)ort 1 ceroreocrtheadd.occurred on page 7, which had obscured the lengthofexposure. This has been Distribution SSppoonnssoorr''ss MRoepnrietsoerntative Study Director MMsrRDRoPbailmdeerau 21ccooppiyes MeP Wilkins copy 4 QCSALMMaannaaggeerment Representative MMrrsMRMHBrBoeowkes 1Tecooppyy Environmental Laboratory Project Number U2723 Page 3of 5 000005 [FPONSOR: J 6) CSL Study Number HTS602 E3nMviCroornpmoernattailonLaboratory S9t35PaBuul,sMhiAnvneesnoutea 55106 Usa [SPONSOR'S REPRESENTATIVE: Ms Rochelle Robideau [LNODCAATCIOONPYOFOFSTTUHDEY,FIRNAAWL DATA REPORT: SCeanntdraHlutStcoine,ncYeorLka,boYrOa4to1ry1LZ UK SLSTUDY NUMBER 3 T ITEM: 4 [sTUDY: T3602 PerFfOluSo)rooctanesulfonate, Potassium Salt (PPeFrfOlSu)o:roAocntaacnuetseuolrfoanlatteo,xiPcoittyassstiuduym wSailtth the honey bee [NOMINAL TEST CONCENTRATIONS: 0Ne9g9a1t,iv0e45co0ntarnodl,0P.o2s0i5tipvge caonbroel, 4.78, 2.17, EST DATES DReafnigniettievsettes1t2:-1251-S2e4ptSeempbteermb2e0r002000 [LENGTH OF TEST: [LENGTH OF EXPOSURE: 72 hours 4 hours T ORGANISM: FONEY BEE (Apis mellifera LJ 42 $ OURCE OF TEST ORGANISMS: oNast.ional Bee Unit Sand Hutton, York YO41 1LZ = LIFE STAGE OF ORGANISMS: Adult ORAL LD, (72 hrs): I[NSOTOATBISSETRICVAELDLYEFDFEETCETRLMEIVNEELD ORAL (BASED ON MEAN INTAKE): (095%p4 gCLai00 s.b3e3s- 0.48 ig a bee) 021 pg aise Environmental Laboratory Project Number U2723 Pagedof 5 scoc0s I; CSL Study Number HT5602 conducted from 12" September to 24" September 2000. The protocol, protocol amendments. OBJECTIVE EXPERIMENTAL DESIGN i aTlhleowosraflorinpgreesctiisoenemxeptohsourdewoivtehratdhmeinhisoturartidonsionfgthpeeritoeds.t itTehmisinreaflqeucetosuosnesumcaroisnepsoocleunttiaoln each dose level received a single dose of the test item dissolved in sucrose. This was pO i St sosSe Page Sof 5 00000 7  { CSL Study Number HTS602 CENTRAL SCIENCE LABORATORY AMENDMENT TO PROTOCOL Study title `Test facility oPrearlfltuooxircoiotcytsatnuedSyulwfiotnhitchAechiodn,ePyotbaessium Salt (PFOS): An acute Environmental R&D Team, Environmental Biology Group Central Science Laboratory, Sand Hutton, York, YO41 1LZ `Study number HT5602 `Amendment number: 2 Number ofpages: ~~ 2 PSatuuldyWiDlikrienctsor . Signature lls.syn, 3 MMaendawgienmBeenwt Representative i3 Signature M Rou0 Date_iy- 3.31 (dd/mm/yy) Environmental Laboratory Project Number U2723 page 1 of2 000008 CSL Study Number HTS602, CENTPRRAOLTOSCCIOELNACMEELNADBMOERNATTORY Amendment No2 to Protocol HTS602 cAhftaenrgecldarfirfoicmatPieornflbuyotrhoeocStpaonnesoSru,lfiotnwiacsAcriedq,uePsotteadstshiautmthSealTte(stPFIOtSem) ntoame be cPehrafnlgueodroforcotma:nesulfonate, Potassium Salt (PFOS) and consequently the study title 3 hPeornfelyuobreeo:octane Sulfonic Acid, Potassium Salt (PFOS): An acute oral toxicity study with the i w 1 hPeornfelyuobreoeo.ctanesulfonate, Potassium Salt (PFOS): An acute oral toxicity study with the `This has no impactonthe Study Circulation list SSppoonnssoorr''ss MReopnrietsoerntative Study Director CCSSLL,MQaAnaUgneimtent Representative MsR Robideau, 3M MMrrDP WPiallkmienrs,,WCiSldLliEfnevIinrtoenmmaetnitoanlalR&D Mr M Bew, CSL Mis R Brookes, CSL. Environmental Laboratory Project Number U2723 page 20f2 0060009 WiigateiorfeeoooIrnstmerenantiroensauls,sevLetrds. ANALYSIS FOR PFOS IN AQUEOUS AND SUCROSE SOLUTIONS USED BY CENTRAL SCIENCE LABORAFOTROCORNDUICTEINSG HONEY BEE TESTING Enclosed please ind a data summary or our screen for PFOS in aqueous and sucrose sluions employed by Central HTS6OU02 Sucrose). Science Laboratory in the honeybee studies (Sample IDs HTS601/02 Water Included. are the rests abl, calibration curve, and. sandardmple. and fon chromatograms fo the sree performed on ur tiple quadrupole LCAMSMS system. Ascanbesen in thedata, thesampleswerelessthanthelimitof quatitai00o5n0,0 mga. (0.000500mg a1.low standard sample ition factor (100). The screenwas condctaend October 26,200. These reals apply to solutions represenofttaosteiuvsed in he Following sic. TPoExiRcFitLyUSOtRudOyOwCitThAtNheEHSoUnLeFyOBNexAT(ECS,L SPtOuTdyASNuSmIbUerM:HTS5A6L0T1)(PFOS): An Acute Contact P`TEoxRicFiLtyUSOtRudOyOwCiTthAtNhEe SHoUnLeFyOBNeAe T(ECS,L SPtOudTyANSuSmbIeUrM:HTS5A6L0T2)(PFOS): An Acute Oral Ra VanIHovYen, Ph Scieist DAT4E/15/ol MANAGEMENT: AndTyp DWiirelctlodr,BA. nNaixyoan,!PChhristy DATE Jil r6e98poComOmeerSckOarmvp+aEeto1n+.72M5oWnono214r01 + TTe o10d-2o29ar4L8501F1 x7517082206332 m7o57: s1colEoxk@viacienptemattionsa com ` 10 mi Em mE Ei EE #OEE EER z ||| L1xh = gg [23 in mn 1a: 88 LEE eS 1 Sie &< oT [rT Page2of4 PiesTo On28 200 1250 `CalbraonFle:CalPFOS 102600 MD Path: Maciniosh HD:APIB000:PROJECT DATAASAC-101:454C101_102600 MD: -WJ _ana 1rSCoonctatao-- ne= sc3o2su5t.1-- 7= 099949 (Comments: POS: 454C-101: Method Development Scrofisehflnakei,wnategr, 8urose LCMS/MS-MAM) ao 4200 x ss00 00a 2400 1800 1200 soo So TOR TR ime zCdo"n wgsaihne. Tah Tih ake -- ori "OCFERTTHIEFIOREIDCGIONPAYL a : oe rR e T 000012 - PaccnTvh,aOttn2156,2000 1269 Page 3of4 C GaumFPiR:aOt GSutP54O0a S1:0110:2Mn 6a0t0h DPar:MoanchSeHtoOrAtPnIfXUG PRO.UwEaCtTtOsNTAsABACOGTA4SR101,102500Mo: Svenrosa rere Rn08o1 sSTOGsmugatl ------------------------------ Theon20 20 1030 N4tte5sa0e1m0t1psasnds " nay: 1200 LNS1 ao4a3dr7uhereo,28s2e0ns .pe , twBlhi 1 .. SSoaaam rin fo Ti% : a SSoanmnewan pi4a4sd7o 2: ses res 133 s mrain onTipe 24-65 o to te h Fh Th A Wh YL Shem EBtOSo2nos' Sot0ugen 13011puts [Aibruainasuns GNS1: roa45a7rhdemreu,208s21rS0e rWitle 1 AvCeo 10 EsetSaar 70 SEhreeew r CT os iegEraioE n Troe A-3388 Th om2a20 100 " . , . 5 a 2: PM oo TShe EWh sy00: 0 ps =o 1 ho Wi We WhEM "OCFERTTHIEFIOERDICGIoNpAyL For, py | ha EE ERED + Bar msm"maedvseAaLmDmIa:mise | 000013 . " "" P`ial:tToFal0:Cda2l F0FO,S1201202850000MO Pa: Page dof 4 HaciloshHOAPS000PROJECT ORTAUSAC-104540101162800Mo: `Commas PFOS:454101:MohdDevlopmon-Seneciofa,wt, ere{LCNSMSMFA) PErOeSS. WATER Thu Onan, 200 122 Roe4e0raatspraocsra Un " 1L:No4as9onT7ivnerse, 200s2ca0ns. o o Gwiaaannt cian 011109 SoArovinn sos) 210 SExajpefctToeideAe0T0 an om :4a Enirin om dtoaggrae:onnYTyipe 3 o r3at8e 66 the 105 138 Uh TW1d6o81a89Bh217 nay: 4000 cos AW 2a06WEN JPoeseitea mss Agnies Reoanrssunars "t:ao4sw7cenunset,209 s2c0ams Saiannntee 111a79 WSeovia) 410 EotonlmamSeSnois in ESntatrrt iTimma. i350 nfgtroogimrniaieonntWTraonien 35 Tw O26 0 I o eo o Pp a . 1040 the sty 40c0p TtR9h0411dHLh tsW aH1o77BT2h14E2U4L46sahsEm "OCFERTTHIEFIOERDICGOINPAYL i 7HAs 15 RECEIVED i Cra AUDIT 000014 + TM T=e J fhde 7 3> ia i LI i i Am J 1 7 i = = > i=ae 1 ind A Ei -=>9 dy A, 5=~ a. TmERAGE CSL Study Number HTS602 PAENRFALCUUOTREOOOCRTAALNSTETOSUXUDILYCFINOTUNYMABSTETERU,:DPYHOTWT5IA6TS0HS2ITUHMESHAOLNTE(YPFBOoSr).: REPORT EPPO GUIDELINE 170 OECD GUIDELINE 213 AUTHOR STUDY INITIATION DATE: 11 September 2000 STUDY REPORT DATE: 23 March 2001 3M Coporsion SUBMITTED TO: Usa Environmental Laboratory 935 Bush Avenue St Paul, Minnesota 55106 Putin borer EnEvnivriornomnemnetnatlalBiRol&oDgyTGeraomup Cental Science Laboratory York`SYaOnd41Hu1t1t2on, UK Report No 1076 Environmental Laboratory Project Number U2723 Page 1 0f.25 ee ee 000015 r= > TM ! ~ i| d |[3 1!a irdo .] > Te oe E= nd ind = a ig da CSLStutduydy NuNmubmebrer HTS602 Si GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT Study number: HTS602 Report tide: hPoernfelyuobreoeoctancsulfonate, Potassium Salt (PFOS): An acute oral toxicity study with the d1.attahegeunnedreartseidgnaerde, vdaelicdl.arAesthsattattehde ionbjtehcetpirvoetsoclaoild ndoowsanmpilnetsheofprdootsoicnogl swoelruteioancshiweevreed caonlldectthaetd the for cgehneemriactaeld ainnaltyhseisa.boTvheestrudeyp.ort fully and accurately reflects the procedures used and the raw data Trehgiuslasttiuodnys was are icnonadcuccotreddanicneacwictohrdtahneceOrwgiatnhistahteioUnKfoGrLEPcoRneogumliactiCoon-sopSeIra1t9i9o9n (No. and 3D0e1v6e)l.opTmheenste (unOdEeCrsDt)andPirningctihpaltestheofUKGoaondd OLEaCboDrarteogruylatPiroancstimceeet(t1h9e97folEloNwVi/ngMsCt/anCdaHrEd:M (98) 17). It is my motion vss cuit i sone wir 1 010 compre, rs, 0 PJraopdauncMtiinoinsBturryeoafu,Ag(rTiocuklytou,re1,98F4o)r.estry and Fisheries, 59 NohiSan, Notification No. 3850, Agricultural crhaawradcatteariazraetiboeninwgasmapienrtfaoirnmeeddinactchoerd3iMngatroch3ivMesS.taTnhdearSdpOopnesroartihnags Psraotceeddutrheats tahnedeMsetthiotdems,isanbdeianlgl crhecehmaircaacltearniazleydsiisn accordance with GLP. No samples of the dosing solutions were collected for Signed: Atif. sie Date:_23J03Jo0) (ddrmmryy) SMtruPdyWDiilrkeicntsor Environmental R&D Team, CSL Environmental Laboratory Project Number U2723 Page2 0f25 000016 Ce -9 9 (CSL Study Number HTS5602 `QUALITY ASSURANCE STATEMENTS Quality assurance inspection of this study wer madeo he Following dts i. Date Activity Date reported 08/09/00 Verification of study plan | . Range test |' 11120099/0000 DPriesppaernastiinogno(ftedsitltuteimonasnd toric reference) |ii 190900 ARDovesesincnsogmmbeeemnesss 08/09/00 1155//0099//0000 15700100 M1a8i0n90te0st Dosing ess 2110900 00900 Asessmens 2110900 | . D2e0f0in9i0ti0ve test Dispensing et tem and toric reference) i2 21/09/00 Preparationofdilutions i[i WeDeoisgisnhginbegeefseders IA 22/09/00 Assessments . 2510900 25/09/00 25/09/00 Palla Wack one215% 5 amy i Mis CV Walker JCa DS oe cr A QA Auditor Tr en tity he Qty Ase sf Cort sire Lira Te smea. Che 1 ako... Dae BR SB SL. amy) *1 ii e CMQerAnstACauldViSteWoiarelnkceerLaboratory 2 FE ps at2s `1 i= Environmental Laboratory Project Number U2723 4 0 00017  2 2 . | 11 i Sx EE = i EE == :8 -- ----- EES i iJ =rTM 9 5| g CSL Study Number HT5602 REPORT APPROVAL SL SPONSOR: 3M Corporation TITLE: Pheornfelyuobreoeoctanesulfonate, Potassium Salt (PFOS): An acute oral toxicity study with the CSL STUDY NUMBER: HT5602 _-- STUDYDIRECTOR: Polity DpATE__23]o3 0 (@dmmiyy) MEnrvPiWriolnmkeinntsal R&D Team, CSL. Approval for Report issue MANAGEMENT: A1 DATE. 233.01 MrM HBew (ddimmiyy) rte Iike Environmental Laboratory Project Number U2723 F Page 4 of 25 ' 000013 EJ . BE] ` - - im a -e hd id I "2 Ed Le le CSL Study Number HTS602 8 TABLE OF CONTENTS GOOD LABORATORY PRACTICE COMPLIANCESTATEMENT... -- arse 2 QUALITY ASSURANCE STATEMENTS... 3 REPORT APPROVAL....cccosersvrvrre SR, serene cssnrsarenees 8 TAOFB CONL TENTES ccm. SUMMARY sss sme] INTRODUCTION. ..cssessnmsssssssssnemmossos OBJECTIVE EXPERIMENTAL DESIGN... sss mm MATEARNDIMEA THOLDS.S.. menor TES HEM rss 10 J CONGO QUIN the SES. 10 Dose preparation ............... resem nese resem rarer -- 1} Doseadministration ................. eeeeseanssans eran ll ODSETVALONS.........oncvrcorerreersen ATBIYOSfGI0S. reseesansne ersersssssas tesa reneessasssseenees LE 12 RESULTS AND DISCUSSION... 12 DIVE 105K... armen 2 T= i= ey `Tabl2e. Oral toxicityofdimethoatetoxicreferencefrom definitive testdata..... Environmental Laboratory Project Number U2723 Page Sof 25 . worsen 15 C0019 > ` i i| i TM Ed TI e i Kind Find E5 EJ Ning -J | i -- - Te = he 8 Ai y CSLStutduydy NuNmubmeberr HT5602 Sh PRINCIPAL WORKERS... DISTRIBOUFRTEIPOORTN.......ccvcrnr DETAILED ORAL TOXICITYTESTRESULTS... 14 pny A---- -- Enommens Liborsory PcNumberUs Pa6gof2e5 000020 - i `: :id i i hi d i ` -|i 3 = iI lTTm E1 N ` ~ - ad = - ioe B= Te TT. = Fm FORSOR [SPONSOR'.S REPRESENTATIVE: est sugy Number irseo2 SUMMARY [IES3 3M Corporation E9n3v5iBruonsmheAntvaelnuLeaboratory SUtsPaaul, Minnesota 55106 Ms Rochelle Robideau LAONCDAATICOONPYOFOSFTTUHDEY,FIRNAAWL DATA REPORT: CSeanntdraHluStctioe,ncoerLka,boYrOa4to1ry112. UK SL STUDY NUMBER. Tre: uDY; INOMINAL TEST CONCENTRATIONS: EST DATES: |exomorTeST: Ata ST ORGANISM: [SOURCE OF TEST ORGANISMS: [LIFE STAGE OF ORGANISMS: T5602 Prterocaneifonse, osmSal (PFOS) P(ePrFfOuSo)r:ooAcntanaecsuutlefoornaaltteo,xiPcoittayssstiuudmySwailtththe honey bee N0ega9tiv9e c1ontar,nodl,00.P2os0.i5tiu4vgeac5.onitbr0oele, 4.78, 2.17, RDeafnigneittievset:te1s2t::1251-S2e4ptSeempbteermb2e0r002000 72 hows HONEY BEE (Apis melljera L] Ncasttional Bee Unit Sand Hutton, York YO41 LZ Adult ORAL LD, (72 hrs): I[NSOTAOTBISSETRICVAELDLYEFDFEETCETRLMEIVNEELD ORAL (BASED ON MEAN INTAKE): 0(4950%uCgLa.0./b3e3e,- 0.48 ug a bee) 021 pgaibee Pag7e0f25 -- oo 000021 . -9 INTRODUucTION CSL Study Number HTS602 SL Behn pe Tan, Ce SeLav 5, "This study was caried out by the Environmental R&D Team,. CentrCaelntraScience Laboratory (CSL), for t2Sh0eepytfieenamabslerarefp1eo0rr2t4s"ausbsSmoeicispasttieeomdnbeworift2h0e0t0i.Ts Tsnhtuedpyporworit.olclobTleh,erperteoastitonceoedlmianmtiehenldCmSbeLe.ntdGi,LspaPowsaerddcahotisfv,ebslylfoCnroLat.emswitioitmlhuentannoodf months after issueof the final report. hs OBJECTIVE ` TPohteasosbijuemctSiavlet (oPfFtOhSi)s asdtumdiyniwsatserto(0etvhaeluhaotneeythbeeeac(uAtpeisomrealllifteorxai)c.ity of Perfuorooctancsulfonate, id EXPERIMENTAL DESIGN hd pb"Treheeecsiosraeanledxiponotgsehusetrrieonnoonvm-erttatrhgeoedt&-wihintoshuercatdsd:omsionirinasgltpreairtnitioaodkn.eofTofhtihcseornteetfsaltmeciittnseamtoeniden mafqaouioendopu(soponlstluecinr,losnreeocusttoaelruotficocen)xp.aolsluoArwtes tffhooerr -- iudnnoitsteeisoattefidotnhsoeufctrthoesset.efstemthrdeiesbcavtcehdesinofsubcereossein grTohuipssvoafs10mobevsesdat aeafcehr d4osheoulresvelanrdecreeivpeldacaedsiwnigtlhe -- "sTeopadreatteerdmibnyeaanfacatpoprroofxtiemnatewetroexicaidtmyinarisatnegreedfi1n0dgirnoguepsstofw3as0pyeorufonrgmeaddu.ltFowuorrkdeorsehsonoefythbeeeess.t Ffievme > gdweeootrmekeretmrriincheaodlnleiynytshbpaeatecsveid1l0ddoduseeefsitnooefhittghhheeletDevisetlos.iotfeTmmhoewrteartleoixtityc.hietnyThaoedfrmeitfnhioesrtteaersnteodtihteforemtgersctoouusplesdcoonfnodt30mbaeyionsuetnecgsu)radewullayt - tchuerireadngoeuteswtitahnudsisnegcoanldomwaeirndotseseto(fhetresetaftteermr(eifsesrureedd atso aa5prtohteodceoflinaimteinvedmteonxitc)i.tyOtnelsy)tahre rerseuplotrsteodf B-i - bh2ey0re0.cualAclholfgdrtahtoeauptaersotfrtebeteamsisniencdaSlocOnu%ltahtweeids,vtusduMycoriorlsae..itAAyfianinited4iaShtuibsonltoehfentatheesteesfftof,oedetawcamhsegreremousepcveoesfds1ea0nnbaeeths5ewdhaooseseoaftfaeknreend = afusrstshserme2n4tawnads4m8adhersatafaerfurrtehmeorv7a2l horfs faefetdeerrsr.emoInvatlheofratnhegstetsetstfeaednedrsd.efiAnliltidvoesetsestofatnetadedimtioanrse -a Fonsdts abnysr.emainintgiveivegrbes eweree nkiled asndbyafvreeezeinng aandoinctinefraotendasa sonnet ia oe Cie x Le Environmental Laboratory Project Number U2723 = Page 8of25 000022 - 9 i CSL Study Number HT5602 al i "The nominal doses administered are shown below. i }' RangeDosfeingdrinoguporal test dose lePveFlsOS Dimethoate g aixsbee) (ug ai/bee) T 2 Te es T T o oss| se FF Control 1 1 50% wiivwsucrose | ois 50% woiv ssucro | | - containing 5% acetone containing - Triton X-100 [Control| So wivserose |na] 3 Definitive test dose levels - - Dose group. (ugPaFiOsbSee) g 3 FO - 2 mT on sp To - ser os 7 0450 [os - - Co5 ntroT l 1 50% wo iv sucrose1-- 5s 0% wiv su-- crose containing 5% acetone. containing - "Triton X-100 - SO%wivsuerose |Na] B-i E] . Tr i.l Cii . BNn =J dEsettiemramtiensedofbyLa Dstavtaisltuiecsalweevraelucaatlicounloaftetdhefomrortahleimtayidnatoar.al test, The no observed effect levels were fNoergzmaautliavteicoonntnroomlisn,aslollyvecnotntcaoinntirnogls4(050%glac)etwoenree) maanidpntoaipnoseidtivecocnocnutrrrolengtrlogyruoptusposc(hdeicmketthhoaattteheasbBeeAsSFwe4r0e reacting normally to the test item during the studies. MATERIALS AND METHODS oTcefhdePulrmaeenstthsPopredocsti,feicsetpdieocininePsErPuosPdeuOdctGasunidodnerloHiuotneeneo1yf70Ba,demeGisun;iidsaetnlrdiantteihooennOdeTEseCcstrDiMbTeeetdshtiondGtsuhifidsoerplriEonvteaolc2uo1al3t,airnHegobntaheseyebSdeieduoep,EofnAfcepucrttose`OrwroaauslpcsTo.onssiiNcdoierryoetdTheesvrta.lpiodItnewnoittrihda:elras)toocuorcncotenrstorloofmobbriiatasa,liabtreyeeeosxfwpe1erc0te%eidomrp0alretasifs;afelacltynddhbiestrrtiehbesuutceasdlcotufolattthreeedasttdmuiedmyne.ttahTnoadhteceosLnDneea!, fell within the OECD standard, i. 0.10 0.35 pg aifbee. Le A Environmental Laboratory Project Number U2723 = Page 90f25 ~ 000023 BJ : :ii '||| | :: : ;1| |:| ! |J = | la- | 2 |i 4=a]d |.-_: =z = : -z = o=s = = is = = _. ) (CSL Study Nu Test item ebC Lsimonnd ewneesraseobxo, inmoaiitnlyof9rmhsesmtn--nilny oe sna dLDrs clHl =abiilo Tor aS- Hi E- - contnaoulydu es beyPFOS (we, anc ah peple of ole`sdtudyy washepiadecafaluiornooro pWoislsde mne - anr al = Lt a o=r | iquelylz -- wr (anal:ytical grad :nddeydbways. r,at a :2 16E- A whiny : 5= py out, cise.oduwy TM recommer = i30EpriS 0 o2poe a n no= eHSp Eo= d n forebuss= to= iolidky= solubil = P(aFpOpSr= oxwe-- ismalte persa ed He r (anloana leya:t cy arrT ied Homa ogen. i thisi rej . :i 3 vigriael whnic eity dof i.checTkree homoge a ne =E ot ise. All - 1 siontes xdisper rorn E --wS --i ohnoo= ff 8= M6 i4ggs: aoa .p fo. : raftthee =re. was. o. n. The approxi c asl:ight rs N prior -un ph bony --= -- ote st). ful Pros = 2 > Ho ez (imate: ania Han the highoshtadslc eddooe Di fanz orded Ye`ork ECformul |wasA-- s%orweidtao6xGioc.snt= ct A ose. m to mae i rec :5 Rdsn daitanTn miatby Siei ts SEeESE BASH 2s TThe ehehnosn)eybbeinetapi ee i. ize p- oitseals: o a is an orom oo onan pon or - impgoraondstnemovao.ih t thectoelsorniesbel } i - tegoriz WTorriorkktei eSns fHoeney= beesri(seepiosa n therepffecairinne, ne 5 ighout A = moe, re Th= -e co= lon 8:loth= e CSL.-- NaMi= ond :Bes Co ao e= ehsad n4otmphfeipn32dra) were Wisk isting fro owes = king as, trom - i : tbhoem (40-50/ebybeuesinr ated wit Qfacar pe oy a, EreE the _ . ei 73 "GungooreAoJou d ta cleans,an.weell Ee Teostw.Pbeesrinte 3i1olz pets " : == : res anc S\aupcprreoxhiomuastseelyd iir n tes hoy - = = } -: _| boemeairinintghe pot Huss lairon BY o:ne aaperka 'rounpa = a ii:sNiyelo= y= duS r.ine gTetml hie te ge> sh= 2yhda Envi in the envi A6545708 amber Nu es a goss.shies ronmer ry Project borator o 00024 ge 10of 2:5 tad .i3 i ile | ip | 1 i ` le 1 lie? i i we i TM +le 7| . Je i " i =1 i i -- = ce Ce a Ty =J = ----. _ =. EEE. `Dosepreparation CSL Study Number HTS602 (S5 4D7o.s8esigofatihelte(sdtefiitnietmivweerteestm) aPdFeOSbyanpdrempaarkiinnggaa ssoelruiteisono fodfil1u0t3ionpsg far.oim/utlhs(.r aDngoes-efsi nding were ptersetp)aarnedd in acetone and then further diluted in 50% wiv sucrose for dosing. In the range test and definitive test 0.5 mlof each acetone solution was then diluted to 10 ml with 50% w/v sucrose. givinga final acetone cOthoEencCoerDnatlrgautteisidtoenldioonsfeess5.%(.1O%n)eAlitstehwtoaousfghrceoqtnhutiirsroelcdonbtceoeenestnrswauatrsieotntrheeoaftheaidgchewetisottnhectowhneacseSnatgmrreaeattileoernvestlhraoenfmaraicenecetodomnimeneinsnodleSudtOi%oinnwtfihover sucrose sucrose (0.5 mi acetone in 10 ml sucrose) and a second setof control bees was treated with S0% wiv Stock Triton solutions X-100. of the positive Stock solutions control, of 2.80 dimethoate, were prepared in deionised water ug ai/ul (range-finding test) and 3.00 pig aif] containing (definitive 1 91 test) dmiamdeethionat1eg/iln TdreiitoonnisXe-d10w0a.teTrhicsonsteariineisngof1dgi/llutTirointson(0X.5-1m0i)0 wweerree tphreenpafruerdthaernddialusteerdietso-o1f0dmiliutwiiotnhs 1500%10wmilvwsiutchro5se0.%Cwo/nvtrsoulcrboesee.s wTehree edsotssedwewriethca0r.r5iemdlou1tgi/n TparriatlolnelXw-i1t0h0tihnosdeeifoonriPsFeOdSw,ater diluted `PSEaOmpSlebsacokfgtrhoeusnudcrloevseelssowleurtieonfouusnedd. in the study were analysed by Wildife Inemational Lid. and no `Doseadministration "The `onto bees filer were anaesthetised paper and counted with carbon ino the petri ddiisohxicdaegeim(mdreodnieastwleyrebedfiosrcearddoeds)i.ng and Each gently group tipped out of 10 bees swmaaslolf,fperree-dw0ei.g2hemdi,ogflasasgfieveednercownictehnitnrtahteiocnag(eoruscionngtraolvsaraisabalbeovveo)l,umtehepidpoesttee.beTinhgismiesaesquurievdaleinnttotao c2a0geusboef.10Abteees.achDodsoessewfeorretahedmtiensitsitteermedawnidthdiinme2thhooautres oafndprefporareaaticohn.control there were 3 replicate Aarfpetpmeraroixn4idmeahrtooeuflrysth3ethmsetludgy0l.a%ssThwfe/eevddoearsqseuecwooeunrsseusmurceerdmooswveaesdsodaetnhtadetrmwbieeneiesgdhhebadyd caconomdnptaitrnhieusoousnsucoarcfocsteehsesfweteeodiesgrushcrofoifsletlehfdeordwoitsthehe remaining in the glass feeders with the weight ofa known volume of the test solutions. Observations Mortality and any bees knocked down, i. alive but immobile, or sumbling were assessed when the hfeoeudresrsafwterrtehrestmaortveodf tahnedt2e4s,, 48 and 72 hours after removal of the test feeders (i.e. 4, 28, 52 and 76 Environmental Laboratory Project Number U2723 Page 11 of 25 N 000025 oe Co 8 i[EY [I 3 i| ii i i| Ilde i i `i I i J I. i ii J =1 -in ! i; -- = EY .- " a= == Te ie ==_ = CSLSttuuddy Number HTS602 S Abe Analyofsdiatsa com tin pot ory es ste 2472s si tlhoegsae.ritThomxiocfidtoyswea(sCeSxLprPersosbeidt a|spLacDkagei)n.iAg a.l.eapstr-bseqeuawrietsh rtehger9es5s%ionco(nFfiindneenyce19li7m1i)ts,waNsOfEitLtedantdo vloepset of herespons cure. The NOELS (no obsarved afc vel) wee estimated sing Stden's cTahteegoLriDes uwseads wueirde:10 classify th toxic of the tet tem according to the ICB (1985). The Highly toxic: MoSdliegrhatltyeltoyxitcoxic: Virually non-toxic: less than 1g aifbee 11-01-010u0gaipfgbaeief.bee: greater than 100 pig ai/bee Data for the dimethoate 24-, 48- and 72-hour mortality results were analysed using the CSL probit program (Probiit 1, version 4). RESULTS AND DISCUSSION AThpepereisualt|s| value fotrhe Fof utheeortaalitlesstcseargeivseunmbmealrowi.sedThien honeybee of 0.40 ug a.ibee. oTalbloesi1 ianfd h2eansdtdfeteamilerdesrteseudltisn are72listeLd Din Rangetest Mortality between a wmaesanobisnetrakveedofat1.0 all doses and 10 from mean pig a.i/bee. Siunbtlaekteha1l.0efpfiegctas./wbeeree owbistehrvaedstaesepkndooscekdroewsnpoannsde stumbling at 4 rs at the two highest dose levels with these individuals dying by 24 hrs, Definitive test s`Tpheornesweasbesitgwneiefincaantmmeoarnaliinttyakae aolf d0o.s4e5saanbdov2e.2a pmgeaani/ibnetea.keofT0h.e2172jihgraL..Dbieewwaisthcaalcsutleaetpeddosaes 0H.o4u0rspgbaasiedbeoen (m9e5a5n% cionntfakiedednacteal(iSmittusd0ent.'s-3t0e.s34t8pu<g0.0b5)e.e)S.ubTlehtehaNlOeEffLecwtasswe0.re21seiegnao.b/sbeereveadt 7a25 Knockdown atthe highest dose level a 4 hrs with these individuals dyingafer24 hours. The 24, `Table 2. 4Mo8rtaanldity72of-hboeuers contact exposed LtoDthevatlouxeisc rfeofrerdeinmceethionatthee itnesttshealdleofiwneidticvaelctuelsattiaorne osfhaonwnorailn LTDhesoefre0su.l1t2s spghoawi adixmeithoiateefviecle wati24i-hhouersraanngdes0e.1p1ourgdaibyditmheethOoFatCeD/bege uate48sansdhoTv2i-nhogurhse. 5were reacting normally inthis test. Environmental Laboratory Project Number U2723 Page 120125 000026 ad i CSL Stutduydy NuNmubmebrer HT5602 iI CONCLUSIONS iIs i Exposure significant ofmorhtoanleiytybeeasbovtoe PaFmOeSanbyinotraakle doofsi0n.g21repsgultiedbinea steep and a d7o2sehrreLsDpuognsecalcouurtvaeiedwitaho i i 002410 higgaaii/bese.ibt7Te2hheesN,OEL based on statistical analysis of the data (Student's test p<0.03) was i| These results demonstrate the oral toxicity of PFOSas 0.40 iga./bee and according to the ICBB i (1985)is classified as highly toxic by ingestion. i iw I sble1 01al LDofP:ROS from definitiveest du Time Hrs LDy ig aibee) 95% Confidence | Estimateof slope of 4[aT om Interval for LD response line 060-085 [So | TT 0s |> LT oa om-0s a7] 033-04[ 8 as i i? - ab2,Olra eoxicityof dimethoute oxic Time LD, refferomrdefeinintivecteset 95% Confidence dat | Estimate of slopeof | + Hrs 24. jig aibee) Interval for LDs, response line i! L8 mTT o oi n 0.10-0.14 oom6o-o0oi3 o5s1 i i REFERENCES = i European and Mediterranean Plant Protection Organization. 1992. Guideline on Test Methods i Jor Evaluating the Side-Effects of Plant Protection Products on Honey Bees. EPPO Bulletin " 22,203:215. i' za Finney, D..J. 1971. Starstical Methods inBiologicalAssay, 2nd ed., Griffin Press, London. = fy International Commission for Bee Botany (ICBB). 1985. Recommendationsfor harmonization of methodsfor testing hazardofpesticides to honeybees. Third Symposium on harmonization of ae methods for testing hazard of pesticides to bees, England. nd Ee Te =. = 2 --_ Organization for Economic Cooperation and Development. 1997. Guideline 213 Honeybess, Acute Oral Toxicity Test. Environmental Laboratory Project Number U2723 ee aPlacgoeo1l3 0f25 000027 hd 2 i i' i| i| i i i i |i | i i i im | i - : E-9Y } - - Ze =e Ce . . = =] --. "ia IS. est sway Number irseoz [fey : PRINCIPAL WORKERS STUDY MANAGEMENT Study Director CSL Management Representative MrP Wilkins MrM H Bew OPERATIONAL SUPERVISORS Principal Scientist Scientist & Test Item Controller ScSicieennitsistt & Deputy Test lem Controller Scieniist Scientist Dr HM Thompson Miss UB Pietsch MMirsAs SHCBaGttreeresnby DrRJ Spinks Mr MJ Kelly QUALITY ASSURANCE QQAA MAuadniatgoerr MMrrssR MCV WBarlokoekres DISTRIBUTION OF REPORT `SSppoonnssoorr''ss MReopnrietsoerntative SCtSuLdyMaDinraegcteomrent Representative QA Manager MsRRobideau MeD Palmer 2 copies 1 copy MMer PMWHilBkienws 11 ccooppyy MrsRMBrookes 1 copy Environmental Laboratory Project Number U2723 I Page 140f25 oo ee 00002 8 wo : i I i i i| i1[ i | | iI 11 i i 1i i 1| 4- 1 i Ji a i = Ce = EE Se ~~ = = CSL Study Number HTS602 gh Table AL Nodmoisen!al (ug ad/bec) 103 110033 APPENDIX 1 (TABLES A1-A8) DETAILED ORAL TOXICITY TEST RESULTS Results of range-finding oral dosing tests with PFOS - Mortality (Aucgtuaailddeosde)' CNaog.e R Cumulative numh ber dead (n=10) 4h 24s 48he 72he % 46 5 10 10 10 90%1 4a7 75 1100 1100 1100 110033 1100 a50 00 1100 1100 1100 103 10 51 0 10 10 10 110038 I10 552 00 0\ 2; 33 103 10 54 0 0 0 4 0.103 010 ss 0 0 0 0 00..110033 00..1100 5567 00 00 00 00 0 o 0 0 58 0 59 0 0 0 0 0 0 0 0 0 0 0 0 0 0 o 0 & 0 0 0 0 o* 0 63 0 0 0 0 ! based on nominal consumption of test solution of 20ul/bee 2abcaesteodnoendaosmeodunt of test solution consumed (average of 10 bees) "undosed Environmental Laboratory Project Number U2723 Page 15 of 25 a oo 000029 oo -- | | i i`ne le re I i 1| 9 i I 7 J i le- i i ii > - ing ii = pi 2i =. Te Te =e == Te =] -- mm CSL Study Number HTS602 al `Table A2. Results of range-finding oral dosing tests with PFOS -- Sub-lethal effects NEdose' (ugaiee) No. (ug aibee) emb(eyrdmisn Ee ww 103 % 6 aK 0 0 0 103 94 47 154K 0 0 0 103 101 48 152K 0 0 0 103 10 x 0 0 0 103 103 10 10 50 51 183K 2% 00 00 00 103 10 2 0 0 0 ix 103 103 10 10 53 54 0 0 0 0 0 1K 0 0 as om 0103 olo so 56 0 0 0 oo o0 0.103 0.10 57 0 0 0 0 0 &0 Seo 0 58 0 0 59 0 0 0 0 0 0 0 o o 0 0 60 0 & 0 0 00 0 0 o 0 6 0 0 0 0 ! based based on on nominal consumption of test solution of 20pl/bee. amount of test solution consumed (average of 10 bes) acetone dosed "undosed Environmental Laboratory Project Number U2723 I Page 16 of 25 -- ee 000030 oo iI p Table A3. CSL Study tudy NNumber HT556600;2 Result of range-finding oral dosing tests with dimethoate - Mortality a [>id Nodmoisen!al (Aucgtauiafldboesde)' CNaog.e (ug ailbee) Find i Cumulative number dead (n=10) ahs hes ash The TM 00.6600 00.5546 31 03 1100 1100 1100 060 060 3 0 10 10 10 7 1 i 030 030 025 030 3 35 2 0 10 10 10 10 10 10 le 030 030 36 1 10 0 10 =TM I o0i1s5 01s 00115s 338 ois 3 00 o 48 5 58 7 85 7 i = | J > TM TM ae 00.007755 00..007745 40 a 0 0 0 0 0 0 0 0 0.075 0074 2 0 1 1 2 00 00 aa 00 00 00 00 0 0 a 0 0 0 0 ig = A. *!bbaasseedd oonn anmoomiunnatlocfotnesstumspoltuitoinonofcoenstsusmoeludti(oanveorfa2ge0polfb1e0cbees) =1i e =J Ze Environmental Laboratory Project Number U2723 Page 17 of 25 000031  i |I ii i i |` i i | i ' i -i 1 =) | i T| T i ie i - SE = Te - "=. = Bid . =a em Table Ad. Nominal dose' (ug aisbec) 006600 0.60 030 030 030 01s 001155 0075 0075 0075 0 0 0 CSL Study Number HTS602 Results of range-finding oral dosing test with dimethoate - Sub-lethal effec= Actualdose (ugaifee) Cage No. Number knockdown (K) or stumbling (5) 4brs 24hrs 48hrs 72hes 005546 231 2xK 00 00 00 0.60 3 7K 0 0 0 025 030 3 3s 1K 0 0 0 0 0 0 0 030 36 0 0 0 0 01s 015 37 38 015 3 0 00 0 00 0 00 0 00 0075 a 0074 41 0 0 0 0 0 0 0 0 0.074 42 0 0 0 0 00 aa" 00 00 00 00 0 as 0 0 0 0 + bbaasseedd oonn anmomoiunnatlocfotnesstumspotluitoinoonftceosntsusmoeludti(oanveorfa2g0ejolf/b1e0ebees) Environmental Laboratory Project Number U2723 Page 18 of 25 . He -------- BE 000032 vr. ii llge Ii s | i* rp iI e| d Ii s TM "i 3 leed | id =| 11 g 1 bo i J . Ng Ce | Bitned = =e -- = =a Table AS. Nodmoisen'al (ug aisbe) a7 447788 217 221177 0991 00999911 aso0.450 0.450 0205 00220055 0o o CSL Studytudy NNumber HTS5600:2 Results of definitive oral dosing tests withPFOS - Mortality (Aucgtuaailfdeosee)' CNaog.e Cumulative number dead (n=10) 4h 2am bs 72h as 48 316 317 3 10 10 3 10 10 10 10 43 318 3 10 10 10 22 22 319 320 1 10 10 0 10 10 10 10 22 El 1 10 10 10 009966 3223 00 036 324 0 os wm 0 045 326 0 045 27 0 87 190 1100 6 9 9 4s 3 7 7 2 4 6 021 28 0 021 329 0 0 0 0 0 i 0 021 330 0 0 2 2 00 0 3m3n1 00 TM0 01 0 01 0 01 0 ooft 00 333345 00 00 00 00 ot 0 336 0 0 0 0 bbaasseedd oonn naommoiunnatlocfotnesstumspotluitoinonofcotensstusmoeludti(oanveorfa2ge0pofbe10ebees) "2aucnedtoosneed dosed. Environmental Laboratory Project Number U2723 oo Page 19.025 oo oo 000033 hd ? CSL Study Number HTS602 SL i `Table AG. Resultsof definitive oral dosing tests with PFOS - Sub-lethal effects In i Nominal dose' Actual dose' (ugaibee) Cage No. i (ug aisbec) i Number knockdown (K) or stumbling (S) i 4hrs 24s d8hrs 72hes ` | 478 478 8 316 8 517 1K IK 0 0 0 0 0 0 i 478 48 318 1K 0 0 0 i i | 21 21 22 22 320 1 0 0 0 0 0 0 0 0 Id Sx 0991 0991 096 096 2 323 0 0 0 0 0 0 0 0 1 i 0991 096 324 0 0 0 0 = 0450 04s 325 0 1s 0 0 |i 00.445500 004455 2326 00 00 10K 10K J- 0205 021 28 0 0 0 0 i ie 0205 0205 021 021 329 330 0 0 0 0 0 0 0 0 l| o o; 0 1 0 0 0 0 --i o0 00 33% 00 00 00 00 - = - a 00 333354 00 00 00 o 0 336 0 0 0 --_ToT b) ased 2 based oonn anmoomiunnatlocfotnesstumspotluitoinonofcotensstusmoeludti(oanveorfa2ge0 u/bee of 10 bees) ==" * aucnedtoosneeddosed Te =. Environmental Laboratory Project Number U2723 = Page 200125 00 0 060034 ~~ i |?| ! i `e i J I i h| d 'i e |EiEnNd i + l | 7 i ihad [ind Bb RE J =e iJ =J = EE =e ae E= a Table AT. Results of definitive oral dosi; ng tests with CSL Stutduydy dimethoat-e NuNmubmebrer HT5602 Mortality Gi SN Nominal Actual dose' Cage dose' (ug aibee) (ugaife) No. Cumulative number dead (n=10) dbs 24hrs ahs 73h 060 060 057 301 058 302 8 2 10 10 10 10 10 10 0.60 0.60 303 s 10 10 10 030 003300 030 304 003300 330056 4 10 10 10 52 1100 1100 1100 01s 001155 01s ois 307 308 1 0 015 309 2 9 76 10 9 10 6 7 0075 0075 [x 0075 310 31 0 0 2 1 2 1 2 1 0.075 0.074 312 0 1 1 1 S00o 0 0 moe 33113 0 0 0 0 0 0 01 ! based on nominal consumption of test solutionof 20pVbee * based on amount of test solution consumed (average of 10 bees) Environmental Laboratory ProjProsect Number U2723 I ----Page 210f25ee 060035 1 ii I1gp i ie Ie le re I i I1 i le 7 ii m i i i i J fi-s i 4 - 1- =| > i. = Te =. =e TWAS Recdeo in tsir h dCiSd LmSetundySi Nubmbern HTS602 SN Nomi Acuntion? Cope dose' (ugaife) No. (ug aibee) E Te ww Number knockdown (K) or stumbling (5) 060 0.60 057 301 2K 0 058 302 6K 0 0 0 0 0 060 060 303 ES 0 0 0 030 030 030 304 x 0 030 305 3K 0 0 0 0 0 030 030 306 x 0 0 0 0.5 015 01s 307 015 308 0 0 0 0 0 0 0 0 015 015 309 0 0 1K 0 00007755 00007745 33110 10K 00 00 00 0.075 0074 312 0 0 0 0 EA 0 0 0 0 313 31 0A0 A00 00 00 based on nominal consumption of test solutionof20plbee "based on amount of test solution consumed (average of 10 bees) Environmental Laboratory Project Number U2723 Page 220f25 000036 Vy 9 | J EJ : ` i ! -i 3`i I --1 ! 2 i -: --4 'e --- = dm -- = Ce -}i Te == Le 4 x CSL Study Number HT5602 gi APPENDI2X INTERIM CERTIFICATE OF ANALYSIS FOR TEST SUBSTANCE INTERIM CERReTviIsFioInC19A7T10E0)OFANALYSIS Cente AnalyticaSlMLaPbroordautcotr:iesPRCOOS,ALRaetfe3r17ence: 023.015 _-- RefPuerirty:enc8#e:65SD%-018 Gime Sedans 1 mew Ee ey ET ---- ------) e el1s (CClPIaMm) T rot r== wT ee--| 23 Msaginemsium 2 "| 2aoonwenaeend [ea55NIPNapomtiaeasmsnisumO'MET :2 Gp T FS m 4S 7. 6o08e4o9nw0iin swst% [[ ReedgCiraoe rLe or e T] n| ToorgTaiacA"mncea:(10) 5 mice 5+ NNuumneo O5 e hmTT 3LziEneBA 231 NCfyeadstotegere i5 sFuieorre [a GrN 0n a Ra eh a aHLn Le aReid FiS haTi hmeER] Cwoh l i pe t ill I5I TTTohhrmeeoerVVVealiiuteeei=01c7%a8l% 5 ThTorwevieid Veineesmoonm | 1EaEnSwAs 45 DS009mwAtns f aree ms" i37 DoSlahowwahnams p 3L 1T 248r weren 54 Sdaimmess oman Perens TFeOams \ o VLCOYRE A a Environmental Laboratory Project Number U2723 Page 23 of 25 C0037 * Ld i i i! `ap }2 Ie le In i| a |' i =I 3| 3 |1 1i i + 7 ng i = l7 o ad = =p =. --9 = = CSL Study Number HT5602 GL CeIntNreTAEnaRlyItMicaCLEabRorTaItoFriIesCCAOTAEROefFereAncNeA#:LY02S3-I0S184 Date ofLastAnsys: 0831100 Expiration Date: 08/31/01 uty 1 of dpi, 45 LOS mpi, ric StorageConditions: Frozen 10C Reassessment Date. 033101 0Fl3u3or%i)de, 0.59% NMR impurities, 13% orgaic aid impurities, 0 38%+POAA, `ToPtuarliitmypur=it1y00f%rom1a3l.lt0e9s%ts==8163..50%9% "Potisaexpesctesdiihoismaltformand thereforenotconsideredanimpurky PobuseryvebdyfDorStCisssgaemnpelrea.llynotsppicabletomataoflow purty. Noendothermwas teres "Sulfurinthesampleappears tobeconverted 0 SOandhencedetectedusingthe deoi tnertmhnienraeo stauilnotir nsonitmnhg eethtSha Oocdlimcn eonnntdtai icltoinosc snsyi.dse`rT,ehedlaenndiimonngrpecsou.nlbtadgcraeeestowelilswimtthetphreesaulifounr. Based NHEBPAA HNoenpoufloorroopbeunotrncicsicidcd PFPA Penafluoropropenaccicd "Theoreical valu calculations based onth empirical formula,CiFSOS (MW=538) Thisworkwas conductedunderEPAGoodLaborstoryPractice Standards(40CFR 160). : conemata Environmental Laboratory Project Number U2723 -- Page 24 of25 meses pr CH > CFTR No [CLLRS] oo B | il Le| i r iyp in i i ry | ili neg i i i i i J i 23g [ini g E-n + vy Z4lee a=J . = CSL Study Number HTS602 Sl ons ay se CeIntNreTAEnaRlyItiMcalCLEabRoTraItoFriIesCCAOTAEROefFerAenNcAe:LY62S3.I0S184 [CC mperiy 7wow arm es ee 1 es] CT Tow 841 NE o:h ThemCtS0Cha id aldtscwiere cSthn sniingn heeC4 de Cste bfileon PrepJ ared By: _TT-- w_ e sire,ncommiies ilLanai connsons ne SRE Tart oft ~~ Environmental Laboratory Project Number U2723 Page 250025 poses