Document yVxjR4nbBKJpO7ZNgpXzb3jn
AR 6 -- 070%
ACUTE TOXICITY TO FISH (FATHEAD MINNOW) IesTsusTa0N 00 GE
Identity: rMeifxetrurreed ctoonatasiPniFnOgSp,erFfCl-u9o5r,ooocrtaansesauclfoomnpaotneewnhticohf FmCa-y20a1lsFo. be (1-Octanesulfonic acid, 1,1,22,3.3,4,4,6,5,6.6,7,7,88.8heptadecafiuoro-, potassium salt, CAS # 2795-39-3)
Remarks: The 3M production lot number was 1. The test sample is mFiCx-t2u0r1eFof(3F-.101%12P9F).OSC,ur3r6e.n0t%indfioertmhaytlieonne ignldyiccoaltbeusttyhlaetthFeCr-,23061.F6i%s waater, e8t.h2e5r%soacdriyulimc sfaolatsm,er3,.07%.0t%risetohdainuomlaomcitnyel,saulnfdat0e,.165.%0%tolsyullftorinaaztoeled.alkyl
cThhaerafcotlelroiwiznegd csounmcmenatrryataipopnlioefsitmopuarimtiixetsu.reDawtiathmianyconmoptlaectceulryately
reflect toxicity of the fluorochemical component of the test sample.
METHOD:
0000000000
Method: OECD Guideline 203. GTeLsPt:tyNpoe: Acute static
Year Completed: 1992
SSuppepcliieesr::PEiCmTe,phSaulpeersioprr,omWeIl,amsaintained at AScl-DETD, Duluth, MN.
Analytical monitoring: Temperature, pH, DO, and conductivity
Exposure period: 96-hours
SStpaetaisrtmiacnal-Kmaertbheordmse:thLoCds.o values calculated using the Trimmed
Test fish age: Juveniles (~30 days)
Length and weight: Length = 0.8-1.6 cm with a mean of 1.1 cm
Loading: 0.13 g/L
Weight = 0.001-0.044 g with a meanof 0.013 g
TPeresttrceoantdmietnito:nsN:ot noted. Dilution water: Shallow well water from the Two Harbors, MN area.
Dilution water chemistry:
pHHa:rdne8s.s2:5 155 mglL (as CaCOs)
oo
hLoiguhrtsinligg:htCaonodl-8whhiotuerfsludoarrke.scent lights providing a daily photoperiod of 16
000331
Stock and test solution preparation: All test solutions were prepared by
direct additions.
CStoanbcielinttyraoftitohnestdesotsicnhgemriactae:lOsnolcuetion: Described as stable.
Exposure vessels: 15 cm H) with 1 iter
4.5 L of test
rectangular solution.
glass
tanks
(20
cm
L
x
15
cm
W
x
Number of replicates: two
Number of fish per replicate: 10
NWautmebrecrhoefmicsotnrcyendturraitnigontsh:e 5stpulduys:a blank control
Conductivity range (0-96 hours):
334966
-- --
433717
ppmmhhooss//cemm
((c1o0n0t0romlge/xLpoesxuproes)ure)
pH range (0-96 hours):
8.24 --8.41 (control exposure) -
7.86 -- 8.36 (1000 mg/L exposure)
Temper2a0t.u7r-e2r1a5ngCe (0-96 hours):
Dissolved oxygen range (0-96 hours):
7.4 - 8.6 mgL_ (control exposure)
4.67.9 mg/L (1000 mg/L exposure)
R0 ESW0 TS 00000000000
Nominal Element
concentrations: BK control, 120, value: 96-hour LCso = >1,000
m2g0/4L,
346,
588
and
1,000
mg/L
96-hour NOEC = 588 mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test SnoutbsttheanfclueorRoecmhearrmkiscaflieplrd.opoTrhteiovnaalluoener.eported applies to that mixture and
co0 nclu0 sions0000000
The FC-201F 96-hour LCs, for Fathead minnow (Pimephales promelas)
wCoanscednettreartmiionned(NtOoEbCe)>w1,a0s0058m8g/mLg./LT.he 96-hour No Observed Effects
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
p0 ataQu0 Aury 000000
000332
Reliability: acceptable
Klimisch ranking level at 24 and 48
3. Dissolved hours for the
oxygen highest
fell below an test concentration
in
one replicate. definitive test.
The range finding test results did not agree with the. The range finding test resulted in 100% mortality by
24
9h6ouhrosuarts1a,t010,00m0g0/mLg,/wLh.erTeesatsintghewdaesfinrietdiovenetetsot dheatderomnliyne3if0%themroretiasliaty by
vreollautmioensohfitphbeedtiwlueteinontwhaetseurr/fatecsetasroeluaotifont.heThexepsoasmuprelechpuarmitbyewratso tnhoet
properly characterized and the study lacks analytical confirmation of the
`amountoffluorochemical proportion in solution.
R0 EFERENCES0 ~0 ~~ 00
This study was conducted by AScl Corporation, Duluth Environmental Testing Division, Duluth, MNat the request ofthe 3M Company, Lab Request number K1792, 1992.
oT0 HWER 00000
Last changed: 6/26/00
000333
EoAoiCirnatTpoioobmrnekis TDey 00150
STUDY TITLE FATHAECAUDTMEITNONXOIWCI(TPYIMOEFPHFACL-E2S0P1RFOMTEOLAS)
DATASTANDARD ~ OECD GUIDELINE 203
STUDY COMPLETED August 24, 1992
TESTING FACILITY AScI-Duluth EAnvsicroCnomrepnotarlatiToensting Division
112 DuFlaustth,seMcNond55S8t05reet Tel. No. (218) 722-4040
STUDY IDENTIFICATIONNUMBERS AScI Study IDF 5030-015-03
3M Company Lab Request# K1792
pSep3otCoaemnpry
1
000334
ASSARapCvDoFTiaOtSFpognDADiRsoanOe "A DrS0T 50)
1.0 GENERAL INFORMATION
[stuay mitie
Acute Toxicity of FC-201F to
Fathead Minnow (Pimephales promelas)
Data Standard
OECD Guideline 203.
3M Environmental Laboratory, Building M2N-3E5-50190,6. 935 Bush Avenue, St. Paul,
|| rseppornessore'nstative
LSaubsoarnatA.oryB,eacBhu,ild3iMngEn2v-i3rEo-n0m9e,ntal
I|
9T3e5l BNou.sh (A6v1e2)nue7,78-S7t.452P.aul, MN 55106; ||
Sponsor's Lab Requestf | K1792.
|
Testing Facility
AScI Corporation/AScI-Duluth
1
Environmental Testing Division,
M1N12 55E8a0s5t; STeeclo.ndNoS.tre(e2t1,8) Du7l2u2t-h4,040.
[principal Investigator
[project pirector
Dinesh Vaishnav
Testing Facility Director
| petinitive Test Dates
Donald Mount
July 9-13, 1992.
|
|I
DRaetpaortVaRleivdaiteiwon
and
| retention of Raw Data and Final Report
| Location of raw pata
| and Final Report
Il
24/92 Dinesh/, Vaishnav WnR2e) po- rt 4 SignAat.ure:
[Two years from the date study is completed.
AESncvIiroCnomrepnotraaltiToens/tAiScnIg-DDuivliustihon,
112 55E8a0s5t; STeeclo.ndNoS.tr(e2e1t8,) Du7l2u2t-h4,040. I|
SS orp s3tMyCoBmmpar
2
000335
Post opps: fT orT i
2.0 OBJECTIVE(S)
TCoondceentterramtiinoen)theof9t6h-eh LtCe5s0t asunbdst9a6n-che,NOEFCC-2(0n1oF,obsfeorrvefdatehfefaedct
minnow (Pimephales promelas) under static test conditions.
This study vas conducted according to the AScI study plan
retained by the Sponsor and OECD Guideline 203 (OECD 1984).
[rest
| substance
3.0 TEST METHODS
Properties: FC-201F, orange/red liquid,
relative density 1.1 (water = 1), stable, and miscible with water.
1
Toregsatnisms
|
TCeosnttrco)n,cen1t2r0;ati2o0n4s,: 3408.,0 5(8d8i,luatnidon 1w0a0t0ermg/L,
prepared by direct additions. By 24 h, all test' solutions, except the control, were foamy.
SC(ooPumirmmceeerp:chiaalHleesalstpuhrpyop,mleilejarus.v)eniolbetaifnaetdheafdromminanows
Holding/acclimation: Held and acclimated for w1a5tedarysf,ordu14ridnagysw.hic0%h emxoprotsaelditytooctchuerrdeidlutdiuorning
holding/acclimation period. At test termina-
t0i.o0n0,1-0t.h0e4y4werein0w.e8i-g1.h6t cwmithinalmeenagnthoafnd0.013 g.
Use in test: Did not feed 24 h before the test
rienpitliiactaitoens./teUsstedco1n0ceonrtgraantiisomns./replicate and 2
S Sr TCoE m
3
000336
A EACrorei0nTB o1t5cSDDraouik A4T S158
Dilution Water
Source: Shallow well water from the
Two Harbors, Minnesota area. The most recent
{
chemical analysis is in Raw Data Package.
l C| hEaxmpobseurrse
test. Characteristics: At test time, the water had a
hardness of 155 mg/L (as CaCO,) and a pH of
-
8.25. Aerated for 24 h prior to using in the
Tmyepaes:uri4n.g5-Laboruetcta20nguclmarlegnlgatshsXta1n5ksw,idtehachX
|
15 cm height.
i
throughout the test. Use in test: 1 L of dilution water or test
solution/chamber. During the test, kept
I
covered, except when experimental observations
:
were made. Gentle aeration was provided
Incubation
Duration: 96 h.
1
Daily photoperiod: 16 h light and & h dark, l
maintained using cool white fluorescent lamps.
l
Temperature: 20.7-21.5C.
|
| Observations | Boiroglaongiiscmasl:wit3hh,abn6orh,malandbedhaaviiloyr- anTdestsigns of I
I
stress, and dead test organisms. Removed dead
I
test organisms when observations were made.
|
Water chemistry: (1) At test initiation- total
hardness and alkalinity, (2) daily-
`temperature, DO, and pH, and (3) at test
initiation and termination- specific
conductivity. All determinations were made
according to APHA methods (1989).
Endpoint Calculations
LC50: Trimmed Spearman-Karber method (Hamilton |et. al. 1977).
NOEC: Dunnett's procedure (TOXSTAT statistical
L1
s1o9f89t)w.are, Version 3.1, University of Wyoming
|
fspoommteMtCiaomrpiy
4
00033;
astComminisns "ha l ty t orS300150
3.0 RESULTS
T(ePsetrtSiunebnsttadnacetaToaxreiciitnyTable 1) 24-h LC50: >1000 mg/L.
1
48-h C50: >1000 mg/L.
.
72-h C50: >1000 mg/L.
96-h LC50: >1000 mg/L.
RPaanrgameesteorfsWater Chemistry | I
l|
_
96-h NOEC: 588 mg/L.
mTgo/tLa)l: ha1r55d-n1e6s0s. (as Caco,
A1l25k-a1l4i0n.ity (as caco, mg/L):
Temperature (C): 20.7-21.5.
Do (mg/L): 4.6-8.0.
|
pH: 7.86-8.54.
:
| (Spuemchiofsi/ccmc):ond34u6c-t4i3v7i.ty
4.0 TEST QA/QC
[onjec criteria i}
|loata
7|
mMuosrttanloittybeinmocronetrtohlanex1p0%o.sure | 0% mortality.
tTlheeesatstltoe6w0re%msitonfaDtOtihocenonmaciuersnttrsaabtteiuoratnatiaotn| ms|TeahatesuurlarotewideosnwtasvDOal5u3ce%onaoctfentt2h1re.a0taiCio.rn |
[value at test temperature. Test duration must be 96 h.
The test duration was 96 h. ||
|
TReesfteren(ctehisSutbessttancies
oTpotxiiocniatly).
| rFeicneanltrteepsotrtwiftohr stohdeimuomst chloride is in Raw Data
-
I
_| package.
_
|
pSomp ieCoto miperr
5
000338
ABSAsoR tCvorem DpoOrTG1va5is AoFgsiDnrtooent cl ty 0S000
5.0 DEVIATIONS
[| 1. 1
TT4hh8eehDdOeiandfoenlfelishbreewplloeiwrcea6rt0e%emooovffedtthheeandahiiragehressaatttiuortnaetswitaosnconaadctjeun2st4treahdt.iaonnd. |
6.0 ADDITIONAL STUDY
dIinluthteionrawnagtee-rfinidnin1g-Ltebsotr,osiwlhiiccahtewasglacsosndbuecatkeedr,usi1n0g0%1 L mHoowretvaelri,tyionctchuerreddefibnyit2i4veh atetst1,,00w0hicmhg/wLastesctondsuucbtsteadncaes. | wd1ae,ss00c0rreimpbgeeda/tLebdtefeosartned,sutbohsentlaydnact3ea0.%smAhsoorwteadalrietsthuyaltto,bcyctuhr3erehdra1b0n0yg%e-96mfoirnatdtailnigtytest || |Lhtahde orecpceuartredteastt 1is,00i0n mrga/wLdtaetsatpascukbasgtea.nce. The infor_ mation for
7.0 REFERENCES
>
_--
------
=
|| AMmeetrhiocdasnfoPrubltihce HEexaalmtihnatAisosnocoifatWiaotner(AaPnHdA).Was1t9e8w9a.teSrt.anAdPaHrAd,
Washington, D.C.
HSapmeialrtmoann-MK.aAr.b,erR.mCe.thoRdussfooraensdtiR.mVa.tinThgurmsetdoina.n l1e97t7h.alTrimmed { c1o1n:7c1e4n-t7r1a9t.ion in toxicity bioassays. Environ. Sci. Technol. |
| o1r9g81a/n1i9z8a4t.ionOECfDorGuEicdoenloimnicesCofoorpeTreasttiionnganofd DCheevmeilcoaplmse.ntOE(CODECD). | Publication Information center, Washington, D.C.
UPnhiyvseirosliotgyy oafndWTyoomxiincgo.log1y989L.aboTroaxtsotrayt, VeDrespiaorntme3n.1t. ofFisZhoology | and Physiology. Universiotfy Wyoming, Laramie, WY.
SSorps3uMtoCyBoAmrpRrI
6
000339
Table 1. | fTseounnbiesntaadnce Conen
(mg/L)
[ocoontrol
Afr sT teCirriroa siirns
Acute toxicity of FC-201F to fathead minnows (7. promelas)
Cumulative number of dead organisms and 8 mortality:
]
nN
on
Ten
Toon
fl
JunJnJoen |
I)
[eo 1,000
Tin Lio Le Tie
ie [iw Jim foe | [sen [sen leon loon
`Peccentages are in parenthesis.
EfCfoercrtesapnodndnion-gst9t5a%ctcocnofnicdaenntcreatiinotnesrvwailtsh
[cso ana wore [[szeownn szecss0o
[owes mgr T[osmoeo
954 confidence interval
ToTeoot ccaartccuuttaanimee
| |
Consors
To
Tienes
"CSO values wes calculated based on the test substance nominal concentration.
[E--m
7
000340