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ARZIG-ooSI Ecotoxicity Elements T itle ; 2- PFO S: A 96-H our Static A cute Toxicity T est with the Fathead Minnow (P im e ph a le s p ro m e la s ) PFO S: A 96-H o ur Toxicity T est with the Freshw ater Alga (S elenastrum capricom utum ) P FO S : A 48 -H o u r Static A cute Toxicity T est with the Cladoceran (.D a p h n ia m a g n a ) Laboratory or A uthor Wildlife International, Ltd. W ildlife International, Ltd. Wildlife International, Ltd. Com pletion Date 4/26/00 Typ Robust Sum m ary, F in al R eport, Protocol 4/26/00 Robust Sum m ary, Final R eport, Protocol 4/26/00 Robust Sum m ary, Final R eport, Protocol P FO S : A 96-H o ur Shell Deposition T es t with the Eastern Oyster y (C ra s s o s tre a v irg in ic a ) PFO S: A 96-H o u r Static A cute Toxicity T est with the Freshwater S Mussel (U nio co m plam atus) PFO S: An A ctivated Sludge, Respiration Inhibition Test Wildlife International, Ltd. W ildlife International, Ltd. W ldlife International, Ltd. PFO S: A 96-H o ur Static A cute Toxicity T es t with the Saltw ater Mysid (M ysid o p s is b a h ia ) W ildlife International, Ltd. PFO S: An Early Life-Stage Toxicity T est with the Fathead Minnow (P im ephales p ro m e la s) l PFO S: A S em i-S tatic Life-C ycle Toxicity Test with the Cladoceran f D aphnia m a g n a ) PFOS: A Flow-through Life-Cycle Toxicity Test with the Saltwater /* Mysid (M ysid op sis bahia) PFO S: A Dietary L C 60 Study with the Mallard )) W ildlife International, Ltd. W ildlife International, Ltd. W ildlife International, Ltd. W ildlife International, Ltd. P F O S : A D ietary L C 50 Study with the Northern Bobwhite /V W ildlife International, Ltd. M ulti-Phase Exposure/Recovery A lgal A ssay Test Method !> 3M Env. Lab T h e E ffects of Continuous A q ueo us E xposure to 1 4 C -7 8 .0 2 on >y Hatchability of Eggs and G row th and Survival of Fry of Fathead M innow (P im e p h a le s p ro m e la s ) and Sum m ary of histopathological Exam inations of Fathead Minnow (Pim ephales promelas) Exposed to 78 .02 for 30 Days EG & G Bionomics 4/26/00 Robust Sum m ary, Final Report, Protocol 4/26/00 Robust Sum m ary, Final R eport, Protocol 4/28/00 Robust Sum m ary, Final Report, Protocol 4/26/00 Robust Sum m ary, Final R eport, Protocol 4/26/00 Robust Sum m ary, F in al R eport, Protocol 4/26/00 Robust Sum m ary, Final R eport, Protocol 4/26/00 Robust Sum m ary. Final R eport, Protocol 4 /2 6 /0 0 Robust Sum m ary, Final R eport, Protocol 4 /2 6 /0 0 Robust Sum m ary. Final R ep o rt, Protocol 12/16/81 August, 1978 D ecem b er, 1978 Brief R obust Sum m ary, Final R eport B rief R obust Sum m ary, Final R e p o rts Effect of Potassium Perfluorooctanesulfonate on Survival, etc. 5 (Daphnid reproduction) Pim ephales promelas 96-hour Toxicity Test D ata Summ ary. A Sam ple F C -94-X (Li salt o f f F O S ) 4 8 -H R A cute Toxicity to D aphnia, D a p h n ia m a g n a . F C -94-X (Li salt of P F O S ) Microbics Microtox Toxicity Test. Sam ple: F C -94-X (Li salt of /r PFOS) Evaluation of F C -94-X by O E C D Activated Sludge Respiration i f Inhibition T e s t # 2 0 9 , R e v ie w of O E C D 2 0 9 and B O D /C O D Test Results for F C -94-X , test data sheets (Li salt of P FO S) 96-H our A cute Toxicity T es t on Bluegill Sunfish (FC -99, D E A salt D-* of P FO S) <2} Acute Toxicity to A ctivated Slu dg e (F C -9 9 , D E A salt of P F O S ) Microtox data for F M -3 82 0 (28 % P F O S ) 3M Env. Lab 3M Env. Lab 3M Env. Lab 3M Env. Lab 3M Env. Lab Analytical B io ch e m is try Laboratories, Inc. 3M Env. Lab 3M Env. Lab 2/13/84 3/25/94 2/10/94 2/7/94 7/22/98 7/13/79 Brief R obust Sum m ary, Final R ep o rt Robust Sum m ary, Sum m ary Page, copies of data Robust Sum m ary, copies o f data Robust Sum m ary, sum m ary o f results, copies of data. Robust Sum m ary, Sum m ary Page, copies of data Robust Sum m ary and Final Report 6/4/79 7/24/91 Robust Sum m ary and copies o f data Robust Sum m ary and data sum m ary 000536 > Acute Toxicity to Daphnia m agna for F M -3 82 0 (2 8 % P FO S ) 2i Toxicity to Algae (Selenastrum capricomutum) for F M -3820 (28% PFOS) EnviroSystems of Resource Analysts, Inc.) EnviroSystems of Resource Analysts, Inc.) 3/26/91 June, 1991 R obuet Sunnmery a n d R ra * Report Robust Sum m ary end Fine! R eport Sum m ary Reports T itle Z* Final Com prehensive Report: FC 95 Laboratory or A u th o r 3M Env. Lab Com pletion Type D ate 3/15/79 Robust Sum m ary, com m ents from S tep h en A. Boyd from M S U and Final R ap o n Environmental Monitoring P a rti: Mult^City Study T itle \ Design and Structure of l^ulti-City Study Low Level Drinking W a te r Analytical M ethod Fluorochem ical C h aracterizatioX of Drinking W a te r Sam ples. Columbus, G A (W 2336) \ Fluorochem ical Characterization of Drinking W a te r Sam ples. Pensacola, FL (W 2 1 76) \ Fluorochemical Characterization of Drinkiha W a te r Sam ples. Port St. Lucie, FL (W 2363) \ Fluorochem ical Characterization of Drinking W t te r Sam ples. Decatur, Alabam a (W 1979) \ Fluorochem ical C haracterization of Drinking W ater'S am ples Mobile, A labam a (W 2 1 51) \ Fluorochem ical C h aracterization of Drinking W a te r Sanrfples. Cleveland, Tennessee (W 1 973) \ Draft Drinking W a te r Health Advisory (D W H A ) - P F O S \ Battelle Field Sampling Procedures Review. Colum bus G eorgia \ City Survey regarding Empirical Human Exposure Assessment. Muttl-City Study Multi-City Study. Field R eport for Cleveland Ten nessee and Decatur Alabam a - Battelle Duxbury Activities Multi-City Study. Field Report for Colum bus G eorgia and Port St. Lucie Florida - Battelle Duxbury Activities Final Multi-City Study. Field Report for M obile A labam a and Pensacola Florida - B attelle Duxbury Activities Quality assurance Project Plan for Empirical Hum an Exposure Assessm ent. Multi-City Study Sampling Task A m endm ent 1 to the Quality Assurance Project Plan and A ssociated S O P 's Laboratory or A u th o r Battelle Mem orial Institute 3M Env. Lab Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. 3M Corporate Toxicology 3M Env. Lab C om pletion D a te 5/1/00 Typo Report 4/28/00 2/29/00 M ethod Final Report 2/28/00 Final Report 2/28/00 Final Report 2/28/00 Final R eport 2/28/00 Final R eport 2/28/00 Final Report 7/7/99 Report 8/3/99 Final R eport Battdtle M em orial Institute Battelle Metporial Institute Battelle M e m o r ia l\ Institute Battelle Mem orial Institute Battelle Mem orial Institute 7/9/99 10/26/99 9 /2 9 /9 9 SK14/99 6 /1 6 /9 9 Final Report Final Report Final Report QAP A m m endm ent ECOTOXICITY ELEMENTS This section presents information and test results from a series of ecotoxicity studies on perfluorooctanesulfonates. The information is presented as Robust Summaries, Final Report and Protocol for each ecotoxicity element. The studies performed during 1999 and in early 2000 were carried out using GLP Standards. In contrast, ecotoxicity studies performed during the period 1974 to 1998 were conducted using protocols and analytical methodologies available at the time of the study. In addition, in these older tests, the sulfonated perfluorochemical products were variable mixtures and contained more impurities. Several tests were hampered by the insolubility of the perfluorochemical and results are expressed as "greater than" the measured solubility. Therefore, the data presented in these historical reports may not be reliable. 000538