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CCEENNTTRRAALL AANNAALLYYTTIICCAALL LLAABBOORRAATTOORRYY RReeppoorrtt NNoo.. --.-66 996677._cceues
DaDtaet.e...........~3gug~uSs~t.- A4~1-9L7~88 ........
SSuubbjjeecctt:: IRRDDCC 113377--009922:: FPCC-~9955,/MMoonnkkeeyy
RReeqquueesstCoort:: c_ _o.d~.Ew-e..LLooR~G...-.r.
DDeepptt.. NNaammee .Zxicology..
RReeqquueesstt NNoo.. ASA969550077______
DDaatteedd oe BA eeeeeU acen
R~eeppoorrtt::
Proj. No.3172110004
sasRtRntueuieddmffyyaeelrrsoeoefnfndccieePFeCC--d11933355w77..i--0t0*88h77inIInbbnkyyctoohrerIIrrRReeDDfcCcCittr.wwsaat((ssttfooooaaouRh99i00dGgad)hyda)sa'y.yffsseeTuueehbbddeaaiinccnguugFtteeeelledevRvRieehhnlreegssssuulwswseeevrmrmeeoeolsnnkkuswseeoeeypyrddettaoaonixnxdoiiivccsiieaittlr)ylysd
aiiaannnnndMdiddiamivlavttielhihdseeduuaaandssldlittlleuuyydd'dyywsfsiseuustmtbbtahamnaliriirettnttteeeeeddatsddhaeaggfffaoaoofilririlnnrosaawanntasssaa:llfyye11ssw33ii77ss--d..00a93y2s2..,BEaacc-hhTTThhheeSegrroossfuueeepprreuudcimcmonoangnanttndaadliienvnlleeeiilddvvseerri4swsem.mwrweoaeemnrrbkeeleeoyrsewes,aaeccrhhed
2 male and 2 female as follows:
Group TGroup
Dosage
Dosaqe
Lreevveell
0
Ssuurrvviivvaall Wa
p~
II
00..55 mmgg//0kkgg//ddaayy
a4/4
IxIxI I
11..55 mmggi/kkgg/!dda~yy 4.5 ng/kg/aay
44/4 o4/n4
IV
Senn annss1s
4.5 mg/kg/day
0/4
SERUM ANALYSIS
HMoonnkkeeyy
BBllaannkk oonn MMeetthhoodd
DDoossaaggee LLeevveell
- -
FCc--9955 iinn SSeerruumn ((ppppmm)~
22
7733555mM
00
"
773355884M
00
40
20
77336688F8
0
20
1
0
15
4746633mM
00..55 mmgg//kkgg//ddaayy
115500
77446666F2
."
150
150
774466221M
11..55 mmgg//kkg~/ddaayy
250
775500008F
.
250
275
"
275
ouPOursrecinpseeiuwotlnyy ddieesvveeeslltooippmeeaddteppdyyrrooclloyyssbiiess1mm0eetthh-oodd25%ww.aass uusseedd.. Precision is estimated to be i0 - 25%.
Ex1h1i8b1it
11118811..00000011
ozs 3M MN02343995
~.R.'#6967
- 2 -
August 8, 1978
1t oSEl SSnLII SFRLI 0 S TpE Ss The high levels of FC-95 in the control serum cannot be explained.
m For example, 7355M was run on 2 different days with the same experimental
SH TNI E HR Ee results. Perhaps a total fluorine should be run on this sample to
determine if this level of F is present. See Table below containing
ERR SRR RS liver results for further discussion (Footnote 2).
-
pp LIVER ANALYSIS
-- ------ tt eel 1535.50 si Monkey
Dosage Level FC-95 in Liver(m~)~ FC-95 in Liver(ppm)
7355M
0
3000
50
77336688F
0
11550002~
2@
2~
77446633M1
00..55 mmgg//kkgg//ddaayy
77000000
1i0000
7466F
"
8000
I00
774466224M
11..55 mmgg//kkgg//ddaayy
4455000000
665500
7500F
"
40000
600
77448844MM
44..55 mmgg//kkgg//ddaayy
4400000000
665500
7502F
"
80000
i000
. " The livers were stored in the refrigerator several months prior to
analysis resulting in the separation of some liquid. The high values
Te and physical size necessitates taking only partial livers in which
case the same ratio (estimated visually) of solid to separated liquid was taken for analysis. In addition, recovery of FC-95 was estimated from experiments where FC-95 was added to a control liver.
Decause of a higher than expected value for the control, a 0.133 g ~Because of a higher than expected value for the control, a 0.133 g
liver sample was analyzed for total fluorine. The fluorine value
EE calculated as FC-95 is equivalent to ii ppm FC-95 in the liver. RA BR As time permits, more work should be done to verify these "high"
control levels.
~/ c:R.A. Prokop 236-3
he
11118811..00000022
3M MN02343996