Document xkOJr2DLXx5MOEgwQGV8kwVJ

Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume I of IX Page 1 of 4068 Covance 6329-183 3M T-6295 COMPLIANCE STATEMENT 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats All aspects of this study were in accordance with the Good Laboratory Practice Regulations as set forth in Title 40 of the US Code of Federal Regulations Part 792, issued November 29, 1983 (effective December 29, 1983), and with any applicable amendments. 2 Covance 6329-183 3M T-6295 QUALITY ASSURANCE STATEMENT This report, with the exception of Appendices 7 and 8, has been reviewed by the Quality Assurance Unit of Covance Laboratories Inc., in accordance with the Environmental Protection Agency (EPA) Good Laboratory Practice Standards, 40 CFR 792 . The following inspections were conducted and findings reported to the study director and study director management. Inspection Dates From To 04/21/98 04/21/98 05/11/98 05/11/98 05/14/98 05/14/98 07/20/98 07/20/98 07/20/98 07/20/98 10/19/98 10/19/98 01/18/99 01/18/99 04/20/00 04/20/99 06/02/99 06/04/99 11/08/99 11/08/99 02/28/00 02/28/00 04/19/00 04/19/00 05/02/00 05/22/00 09/20/00 10/12/00 10/10/00 11/03/00 05/15/01 05/17/01 12/05/01 12/13/01 01/02/02 01/02/02 01/02/02 01/02/02 Phase Protocol Review Food Consumption Test Article Preparation Body Weight Protocol Amendment Review Test Article Administration Animal Observation Clinical Laboratory Inspection Protocol Amendment Review Test Article Administration Protocol Amendment Review Postlife Data Review Data Review Report Review Report Review Report Review Protocol Amendment Review Protocol Amendment Review Date Reported to Study Director and Study Director Management 04/21/98 05/11/98 05/14/98 07/20/98 07/20/98 10/19/98 01/18/99 04/20/99 06/04/99 11/08/99 02/28/00 04/19/00 05/23/00 10/17/00 11/08/00 05/17/01 12/17/01 01/02/02 01/02/02 3 Covance 6329-183 3M T-6295 STUDY IDENTIFICATION 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Test Material: Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) Sponsor: 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Study Monitor: Andrew M. Seacat, PhD 3M 651.575.3161 Alternate Study Monitor: John Butenhoff, PhD, DABT 3M 651.733.5180 Study Location: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Study Director: Peter J. Thomford, PhD Covance Laboratories Inc. PO Box 7545 Madison, Wisconsin 53707-7545 608.241.7207 Study Timetable: Study Initiation Date: Experimental Start Date: Inlife End Date: Experimental Termination Date: Study Completion Date: April 15, 1998 April 20, 1998 April 24, 2000 December 21, 2001 January 2, 2002 4 Covance 6329-183 3M T-6295 KEY PERSONNEL 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Study Director: Study Toxicologist: Study Coordinator: Supervisor, Small-Animal Toxicology: Supervisor, Dose Formulation: Supervisor, Chemistry/Tox Support: Associate Director, Laboratory Animal Medicine: Clinical Pathologist: Supervisor, Clinical Pathology: Anatomic Pathologist: Supervisor, Anatomic Pathology: Supervisor, Anatomic Pathology: Staff Scientist, Safety Assessment and Biostatistics: Principal Scientist and Biostatistician, Safety Assessment and Biostatistics: Anatomic Pathologist, Cell Proliferation: Anatomic Pathologist, Electron Microscopy: Peter J. Thomford, PhD Thomas E. Ryan, BS Nii van Bruce-Konuah Pamela D. Gabris, BS, LAT Dixie Bushee, BS, LATG Mark Hoffmann, BS, MBA Donna J. Clemons, DVM, MS Diplomate, ACLAM Robert L. Hall, DVM, PhD Diplomate, ACVP (Clinical Pathology) Ronald Markevitch, BS, MT (ASCP) Richard D. Alsaker, DVM, MS Diplomate, ACVP Diplomate, ABT Kimberly W. Durland, BS, HT Laurie J. Schuller, BA, LAT Arjun Roy, MA Ajit K. Thakur, PhD Sandra R. Eldridge, PhD Pathology Associates International James B. Nold, DVM, PhD Diplomate, ACVP Pathology Associates International 5 Covance 6329-183 3M T-6295 VOLUME I OF IX CONTENTS Page ABSTRACT.................................................................................................................. 11 PURPOSE..................................................................................................................... 17 REGULATORY COMPLIANCE.................................................................................. 17 TEST MATERIAL AND VEHICLE ............................................................................. 17 Test Material ............................................................................................................. 17 Vehicle...................................................................................................................... 17 Carrier ....................................................................................................................... 18 Reserve (Archive) Samples........................................................................................ 18 Disposition ................................................................................................................ 18 TEST ANIMALS AND HUSBANDRY........................................................................ 18 Animals..................................................................................................................... 18 Identification ............................................................................................................. 18 Justification ............................................................................................................... 19 Husbandry................................................................................................................. 19 Acclimation............................................................................................................... 19 PROCEDURES............................................................................................................. 20 Major Computer Systems .......................................................................................... 20 Group Designations and Dose Levels ........................................................................ 20 Dosing Procedures..................................................................................................... 21 Dose Analysis............................................................................................................ 23 Observation of Animals............................................................................................. 23 Clinical Pathology ..................................................................................................... 24 Serum PFOS Analyses............................................................................................... 25 Anatomic Pathology .................................................................................................. 26 Interim Sacrifices ...................................................................................................... 26 Terminal Sacrifice ..................................................................................................... 26 Recovery Sacrifice .................................................................................................... 27 Statistical Evaluation ................................................................................................. 30 Survival and Tumor Analysis .................................................................................... 30 Record Retention....................................................................................................... 33 RESULTS AND DISCUSSION .................................................................................... 33 Dose Analysis............................................................................................................ 33 Observation of Animals............................................................................................. 34 Clinical Pathology ..................................................................................................... 36 Anatomic Pathology .................................................................................................. 37 Cell Proliferation Results........................................................................................... 39 6 Covance 6329-183 3M T-6295 VOLUME I OF IX CONTENTS (Continued) Page RESULTS AND DISCUSSION (Continued) Electron Microscopy Results ..................................................................................... 39 Characterization of Tumor Incidence Outcome.......................................................... 40 CONCLUSION............................................................................................................. 41 SIGNATURES.............................................................................................................. 42 REFERENCES.............................................................................................................. 43 PATHOLOGY REPORT............................................................................................... 44 PATHOLOGY PEER REVIEW STATEMENT ............................................................ 52 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC, AND NEOPLASTIC LESIONS .............................................................................. 56 COMMENTS ON THE DATA ..................................................................................... 93 CODES, ABBREVIATIONS, AND UNITS.................................................................. 96 General Codes and Abbreviations.............................................................................. 97 Codes for Clinical Pathology..................................................................................... 98 Abbreviations and Units for Clinical Hematology.................................................... 101 Abbreviations and Units for Clinical Chemistry....................................................... 103 Abbreviations and Units for Clinical Urinalysis....................................................... 105 Abbreviations and Units for Urine Chemistry.......................................................... 106 Codes for Anatomic Pathology................................................................................ 107 FIGURES 1 Survival Data - Males ........................................................................................ 109 2 Survival Data - Females..................................................................................... 110 3 Mean Body Weight Data (g) - Males ................................................................. 111 4 Mean Body Weight Data (g) - Females .............................................................. 112 5 Mean Food Consumption Data (g) - Males ........................................................ 113 6 Mean Food Consumption Data (g) - Females ..................................................... 114 TABLES 1 Results of Homogeneity Analyses (ppm) ........................................................... 115 2 Results of Stability Analyses (ppm) ................................................................... 119 3 Results of Dose Preparation Analysis (ppm) ...................................................... 121 4 Survival Data..................................................................................................... 132 5 Summary of Clinical Observations..................................................................... 150 6 Summary of Mass Observations......................................................................... 170 7 Covance 6329-183 3M T-6295 VOLUME I OF IX CONTENTS (Continued) Page TABLES 7 Summary of Body Weight Data (g).................................................................... 176 8 Summary of Body Weight Change Data (g)....................................................... 188 9 Summary of Food Consumption Data (g)........................................................... 200 10 Summary of Test Material Consumption Data (mg/kg/day)................................ 212 11 Summary of Clinical Hematology Data - Week 4............................................... 218 12 Summary of Clinical Hematology Data - Week 14............................................. 222 13 Summary of Clinical Hematology Data - Week 27............................................. 226 14 Summary of Clinical Hematology Data - Week 53............................................. 230 15 Summary of Clinical Chemistry Data - Week 4 ................................................. 234 16 Summary of Clinical Chemistry Data - Week 14 (PCOAO) ............................... 240 17 Summary of Clinical Chemistry Data - Week 14................................................ 242 18 Summary of Clinical Chemistry Data - Week 27................................................ 246 19 Summary of Clinical Chemistry Data - Week 53................................................ 250 20 Summary of Clinical Chemistry Data - Week 105.............................................. 254 21 Summary of Clinical Chemistry Data - Day 719 ................................................ 256 22 Summary of Clinical Urinalysis Data - Week 4.................................................. 257 23 Summary of Clinical Urinalysis Data - Week 14................................................ 259 24 Summary of Clinical Urinalysis Data - Week 27................................................ 261 25 Summary of Clinical Urinalysis Data - Week 53................................................ 263 26 Summary of Urine Chemistry Data - Week 4 ..................................................... 265 27 Summary of Urine Chemistry Data - Week 14 ................................................... 267 28 Summary of Urine Chemistry Data - Week 27 ................................................... 269 29 Summary of Urine Chemistry Data - Week 53 ................................................... 271 30 Summary of Organ Weight Data - Week 14 Sacrifice ........................................ 273 31 Summary of Organ Weight Data - Week 53 Sacrifice ........................................ 303 32 Incidence of Macroscopic Observations - Week 14 Sacrifice ............................. 318 33 Incidence of Macroscopic Observations - Week 53 Sacrifice ............................. 320 34 Incidence of Macroscopic Observations - All Animals....................................... 333 35 Incidence of Macroscopic Observations - Recovery Sacrifice ............................ 357 36 Incidence of Microscopic Observations - Week 14 Interim Sacrifice.................. 372 37 Incidence of Microscopic Observations - Week 53 Interim Sacrifice.................. 385 38 Incidence of Microscopic Observations - Terminal Sacrifice.............................. 401 39 Incidence of Microscopic Observations - Unscheduled Deaths and Sacrifices .... 425 40 Incidence of Microscopic Observations - All Animals ....................................... 460 41 Incidence of Microscopic Observations - Recovery Sacrifice ............................. 497 APPENDIX 1.............................................................................................................. 514 Protocol Deviations ................................................................................................. 515 8 Covance 6329-183 3M T-6295 VOLUME II OF IX CONTENTS (Continued) Page APPENDIX 2.............................................................................................................. 520 Individual Animal Fate Data.................................................................................... 521 Individual Clinical Observations.............................................................................. 543 VOLUME III OF IX APPENDIX 2 (Continued) .......................................................................................... 809 Individual Clinical Observations.............................................................................. 810 VOLUME IV OF IX APPENDIX 3............................................................................................................ 1257 Individual Body Weight Data (g)........................................................................... 1258 APPENDIX 4............................................................................................................ 1345 Individual Food Consumption Data (g).................................................................. 1346 Individual Test Material Consumption Data (mg/kg/day)....................................... 1432 APPENDIX 5............................................................................................................ 1512 Individual Clinical Hematology Data..................................................................... 1513 Individual Clinical Chemistry Data........................................................................ 1585 Individual Clinical Urinalysis Data........................................................................ 1652 Individual Urine Chemistry Data ........................................................................... 1724 VOLUME V OF IX APPENDIX 6............................................................................................................ 1750 Individual Anatomic Pathology Data ..................................................................... 1751 9 Covance 6329-183 3M T-6295 VOLUME VI OF IX CONTENTS (Continued) Page APPENDIX 6 (Continued) ........................................................................................ 2144 Individual Anatomic Pathology Data ..................................................................... 2145 VOLUME VII OF IX APPENDIX 6 (Continued) ........................................................................................ 2497 Individual Anatomic Pathology Data ..................................................................... 2498 VOLUME VIII OF IX APPENDIX 6 (Continued) ........................................................................................ 2855 Individual Anatomic Pathology Data ..................................................................... 2856 VOLUME IX OF IX APPENDIX 6 (Continued) ........................................................................................ 3455 Individual Anatomic Pathology Data ..................................................................... 3456 APPENDIX 7............................................................................................................ 4004 Cell Proliferation Report ....................................................................................... 4005 APPENDIX 8............................................................................................................ 4029 Electron Microscopy Report .................................................................................. 4030 10 ABSTRACT Covance 6329-183 3M T-6295 The purpose of this study was to assess the chronic toxicity and carcinogenicity of Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295), also referred to as T-6295, when administered in the diet to rats for at least 104 weeks. Due to excessive mortality, female animals in Group 3 were terminated during Week 103. Male and female Crl:CD(SD)IGS BR rats were assigned to six groups (70 animals/sex/group for Groups 1 and 5; 60 animals/sex/group for Groups 2, 3, and 4; and 40 animals/sex/group for Group 6). Each group received diets containing acetone, the vehicle (Group 1), 0.5 (Group 2), 2 (Group 3), 5 (Group 4), or 20 (Groups 5 and 6) ppm T-6295. Group 6 was placed on recovery after 52 weeks of treatment. Food was provided ad libitum, except when animals were fasted. Water was provided ad libitum. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity; findings were recorded as they were observed. At least once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal was recorded. Body weight data were collected weekly through Week 17, once every 4 weeks thereafter, and at Week 105. Food consumption data were collected weekly for the first 16 weeks and once every 4 weeks thereafter. During Weeks 4, 14, 27, and 53, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from 10 animals/sex in Groups 1 through 5. Blood was collected for cholesterol and triglyceride determinations from all animals prior to the terminal sacrifice during Week 105 (Week 103 for females in Group 3) and the recovery sacrifice (Group 6) during Week 106. Blood films were also prepared for animals at the terminal and recovery sacrifices. Five animals/sex in Groups 1 through 5 were sacrificed during Week 4; livers were collected and weighed. [The liver samples were collected for PFOS analysis, mitochondrial activity, hepatocellular proliferation rate measurements by proliferation cell nuclear antigen (PCNA), and determination of palmitoyl-CoA oxidase activity. In addition, liver samples were collected for PFOS analysis (Weeks 14, 53, 103, 105, and 106), hepatocellular proliferation rate measurement [by PCNA at Week 14 and by bromodeoxyuridine (BrdU) immunohistochemistry at Week 53] and palmitoyl-CoA oxidase determination (Week 4 and 14).] A section of duodenum was also collected at Week 53 for BrdU immunohistochemistry. During Weeks 14 (Groups 1 through 5) and 53 (Groups 1 and 5), necropsies were performed on five and 10 animals/sex, 11 Covance 6329-183 3M T-6295 respectively. Surviving females in Group 3 were sacrificed and necropsied during Week 103 because of reduced survival. The remaining animals in Groups 1 through 6 were sacrificed and necropsied during Weeks 105 and 106. At necropsy, macroscopic observations were recorded, organ weights were obtained (Weeks 14 and 53), and tissues were placed in fixative as specified by the protocol. At the terminal and recovery sacrifices, sections of the heart and liver were preserved in 2.0% parafomaldehyde/2.5% gluteraldehyde in 0.1M phosphate buffer, processed, and embedded in epoxy resin for possible future electron microscopy from 10 animals/sex in Groups 1, 4, 5, and 6. Animals that died on test or were sacrificed at unscheduled intervals were also necropsied, but organ weights were not recorded. Microscopic examinations were done on selected tissues (adrenals, brain, eyes, kidney, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries) from the animals necropsied during Week 14 and on all tissues from the animals in Groups 1 and 5 that were necropsied during Weeks 53, 105, and 106. In addition, microscopic examinations were done on the tissues from animals that died or were sacrificed due to poor health. Macroscopic lesions, liver, lung, kidneys, pancreas, thyroid, testes, and mammary gland (females), and urinary bladder were examined for animals in Groups 2, 3, 4, and 6 that were sacrificed during Weeks 103, 105, and 106. Liver sections from five animals/sex/group from Groups 1 and 5 were stained with Oil Red "O" and examined microscopically. In addition, liver samples from five animals/sex/group were processed and evaluated by electron microscopy. Survival after 104 weeks of treatment was 22.0, 22.0, 34.0, 50.0, 45.0, and 27.0% for males in Groups 1, 2, 3, 4, 5, and 6, respectively, and 50.0, 30.0, 20.0 (after 102 weeks), 34.0, 52.0, and 48.0% (after 104 weeks) for females in Groups 1, 2, 3, 4, 5, and 6, respectively. There was a significant increased trend in survival that occurred in the males that was due to significant increases in survival in mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control group. None of the other treated groups in the males revealed any significant differences in survival. No significant trend was noted in survival in females. There was a significant decrease in survival in the mid-dose (2.0 ppm) group and not in the mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control. 12 Covance 6329-183 3M T-6295 There were no clinical observations attributed to administration of the test material. Males given 20 ppm (Groups 5 and 6) had significantly lower mean body weights compared to animals in the control group during Weeks 9 through 37. During recovery the mean body weights for Group 6 males were similar to mean body weights for Group 5 males. Females in Group 5 given 20 ppm had significantly lower body weights compared to animals in the control group during Weeks 3 through 101. Mean body weights for females in Group 6 given 20 ppm were statistically lower than mean body weights for control females during Weeks 3 through 61, but their mean body weights became nearer to the weights of the control females when placed on recovery. At Week 105, mean body weights for surviving males were 98, 108, 103, 101, and 96% of controls for the low- (0.5 ppm), mid- (2.0 ppm), and high-dose groups (20.0 ppm), respectively. At Week 105, mean body weights for surviving females were 101, 107 (Week 101), 100, 86, and 105% of controls, for the low- (0.5 ppm), mid- (2.0 ppm), and high-dose groups (20.0 ppm), respectively. Mean body weight change was statistically significantly lower for males given 20 ppm diets for Weeks 1 through 15 and Weeks 15 through 29. Mean body weight change was statistically significantly lower for females given 20 ppm for Weeks 1 through 15 and Weeks 29 through 57 (Group 5 only). Mean body weight changes for males and females in Group 6 were significantly increased during the several weeks of recovery. Animals at lower dose levels occasionally had statistically significantly decreases in body weight gain; however, these occurrences were inconsistent over time between sexes and were not clearly dose-related. Although not always statistically significant, males given 20 ppm tended to consume less food during Weeks 1 through 24. Food consumption was similar for males given 20 ppm compared to animals given the control material during Weeks 28 through 104. Statistically significantly lower food consumption was noted frequently for females given 20 ppm during Weeks 2 through 44. Food consumption for males and females was similar in all the other treated groups compared to animals given the control material. Dietary administration of PFOS for approximately 53 weeks was associated with mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. 13 Covance 6329-183 3M T-6295 None of these effects were considered adverse. The effect on cholesterol was no longer apparent after 104 weeks of treatment. There was no effect on hepatic palmitoyl-CoA oxidase activity. Terminal body weights in males and females at Week 14 were comparable between control and treated groups. At the Week 14 interim sacrifice, absolute and relative liver weights were significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas in males and females, and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. At the Week 53 interim sacrifice, terminal body weights were significantly decreased in the females given 20 ppm. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. At the Week 53 interim sacrifice, centrilobular hepatocytic hypertrophy and vacuolation was increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated with the administration of the test material. Liver findings in several unscheduled deaths given 20 ppm resembled those seen in animals sacrificed at Week 53. At the terminal sacrifice, the livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in the animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. 14 Covance 6329-183 3M T-6295 The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. In the unscheduled sacrifices between Weeks 54 and 105, animals given 20 ppm had increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, and centrilobular hepatocytic pigment were noted. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. A significant positive trend was noted in the incidences of liver hepatocellular adenoma for males. This was associated with a significant increase in the high-dose (20.0 ppm) group over the control. The high-dose recovery (20.0 ppm) group animals in this case exhibited a significantly decreased incidence when compared to that of the high-dose (20.0 ppm) group. Significantly increased incidences were observed for thyroid follicular cell adenoma in the high-dose recovery (20.0 ppm) group when compared to either the control or the high-dose (20.0 ppm) group. There was also a significant increase in the combined (adenoma and carcinoma) thyroid follicular cell tumors in the high-dose recovery (20.0 ppm) group animals as compared to that of the high-dose (20.0 ppm) group. None of these cases were associated with any significant trend. Thyroid follicular cell carcinoma did not show any significant effect. There were no other significant neoplastic findings in the males. Significant positive trends were observed in the incidences of hepatocellular adenoma and hepatocellular combined adenoma and carcinoma in the females. These cases were associated with significant increases in the high-dose (20.0 ppm) group as compared to the control. All these tumors were incidental and noted at the terminal sacrifice. A marginally significant increase for thyroid follicular cell adenoma and carcinoma combined was observed in the mid-high (5.0 ppm) group but not in the high-dose (20.0 ppm) group as compared to the control group. In fact, thyroid C-cell adenoma and thyroid C-cell combined adenoma and carcinoma actually showed significant decreases in the high-dose (20.0 ppm) group as compared to the control. There were significant negative trends in the incidences of mammary fibroadenoma and mammary combined adenoma and fibroadenoma. These cases were associated with significantly lower incidences in the respective high-dose (20.0 ppm) group when compared to that of the control. However, the low-dose (0.5 ppm) group animals for these cases exhibited significant increases over the control. A significant decreased incidence was found for the high-dose recovery (20.0 ppm) group in the incidences of mammary carcinoma as compared to the high-dose (20.0 ppm) group. There was no other significant group effect 15 Covance 6329-183 3M T-6295 in mammary carcinoma. There was a marginal significant negative trend for the mammary combined fibroadenoma/adenoma/carcinoma. However, the low- (0.5 ppm) and mid-high (5.0 ppm) groups for this case exhibited significantly increased incidences over the control. There were no other significant neoplastic findings in the females. There were no statistically significant increases in cell proliferation as measured by Proliferative cell nuclear antigen (PCNA) immunohistochemistry at weeks 4 and 14, or by bromodeoxyuridine (BrdU) immunohistochemistry at week 53. Liver samples were obtained from each of the 20 rats during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for ultrastructural changes that may be related to chronic toxicity of the test material when administered in the diet. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the high dose group and the control animals. Other changes included a slight increase in the amount of glycogen in treated animals compared with controls. The chronic toxicity and carcinogenicity of the test material, Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) was assessed following dietary admixture to male and female Crl:CD(SD)IGS BR rats at dose levels of 0.5, 2, 5, or 20 ppm. Based on anatomic pathology findings in the liver, the no-observable-adverse-effect level for Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) is considered to be 2 ppm. 16 PURPOSE Covance 6329-183 3M T-6295 The purpose of this study was to assess the chronic toxicity and carcinogenicity of Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295), when administered in the diet to rats for at least 104 weeks. Due to excessive mortality, female animals in Group 3 were terminated during Week 103. REGULATORY COMPLIANCE All aspects of this study were in accordance with the Good Laboratory Practice Regulations as set forth in Title 40 of the US Code of Federal Regulations Part 792, issued November 29, 1983 (effective December 29, 1983), and with any applicable amendments. TEST MATERIAL AND VEHICLE Test Material The test material, Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295), Lot No. 217, is a light colored, free flowing powder and is 86.9% pure. It was received at Covance on June 4, 1996, and September 4, 1997. The test material was stored at room temperature. Information on synthesis methods, stability, composition, or other characteristics that define the test material was provided by the sponsor and is on file with Covance. Vehicle The vehicle, acetone (Spectrum Chemical Mfg. Corp., New Brunswick, New Jersey and Gardena, California), Lot Nos. LH0253 and NS0231 (expiration date: June and September 1, 2000, respectively). The shipments were received at Covance on June 23, 1997, and September 8, 1998, respectively. The vehicle was stored at room temperature. 17 Covance 6329-183 3M T-6295 Information on synthesis methods, purity, composition, or other characteristics that define the acetone is on file with the manufacturer. Carrier Certified rodent diet (#5002 meal, PMI Nutrition International) was used as supplied. The lot numbers are recorded in the data. The diet is routinely analyzed by the manufacturer for nutritional components and environmental contaminants. Reserve (Archive) Samples A reserve sample (5 g) of the test material was taken and stored at room temperature. This sample was transferred to the sponsor on June 12, 2000. Disposition Remaining test material was returned to the sponsor on June 12, 2000, after authorization from the sponsor. TEST ANIMALS AND HUSBANDRY Animals Male and female Crl:CD(SD)IGS BR rats were obtained from the Raleigh, North Carolina, facility of Charles River Laboratories on April 7, 1998. At initiation of treatment, the animals were approximately 41 days old; the males weighed from 135 to 226 g, and the females weighed from 128 to 182 g. Identification Each animal was assigned a temporary number upon arrival. Before initiation of treatment, a microchip identification device was implanted into each animal. After randomization for placement on test, each animal was assigned a permanent number, and the microchip was coded with the permanent number. All data for an animal are recorded under these numbers. 18 Covance 6329-183 3M T-6295 Justification Historically, rats have been used in safety evaluation studies and are recommended by appropriate regulatory agencies. Husbandry Animal were housed in Animal rooms 316, 332, or 345 for 13 days during acclimation and then were moved to rooms 3004 or 3005 for the duration of the study. Environmental controls for the animal rooms were set to maintain 18 to 26oC, a relative humidity of 30 to 70%, and a 12-hour light/12-hour dark cycle. The animals were housed individually (except for the first 6 days of acclimation when animals were group-housed) in stainless-steel cages (see Protocol Deviations for exceptions). Some animals were placed in polycarbonate cages during the study when health problems dictated. Certified rodent diet (#5002 meal, PMI Nutrition International) was provided ad libitum during acclimation (see Protocol Deviations for exceptions). Water was provided ad libitum (see Protocol Deviations for exceptions). Samples of the water are analyzed for specified microorganisms and environmental contaminants. The results are on file with Covance-Madison. There were no known contaminants in the diet or water at levels that would have interfered with this study. Animals not selected for the study (42 males and 40 females) were sacrificed and discarded. Acclimation Four hundred and two males and 400 females were received on April 7, 1998, and acclimated in Animal Rooms 316, 332, or 345 for 13 days then moved to rooms 3004 and 3005 before initiation of treatment. In general, animals in this shipment appeared 19 Covance 6329-183 3M T-6295 healthy. During acclimation, the animals were examined for abnormalities indicative of health problems, and body weights were recorded for all animals at randomization. PROCEDURES This study was conducted in accordance with the Protocol dated April 15, 1998, and with Protocol Amendment Nos. 1, 2, 3, 4, and 5. Protocol deviations are in Appendix 1. Major Computer Systems The Path/Tox application, supplied by Xybion Medical Systems Corporation, was used for the direct on-line capture of in-life toxicology and anatomic pathology data. The Randomization and Data Extension Systems (RADES) and Automatic Form and Label Generation System (AFLGS) applications were used in conjunction with the Path/Tox system to randomize animals and produce labels and forms, respectively. The Talisman application was used for the dose preparation information, and the Millennium system was used for the collection of dose analysis data, and the Clinical application was used for the collection of clinical pathology data. The Report Generation System application was used to pull the information from the Path/Tox and clinical applications into Word for reporting purposes. All version numbers of the applications can be found in the log book for the application. Group Designations and Dose Levels The animals were examined by a laboratory animal veterinarian on April 14, 1998, and were found to be suitable for study consideration. Selection of animals for the study was based on acclimation data. Animals were assigned to treatment groups using a computerized blocking procedure designed to achieve body weight balance with respect to treatment group. At the time of randomization, the weight variation of the animals did not exceed +2 standard deviations of the mean body weight for each sex. Group mean body weights were analyzed using Levene's test for homogeneity of variance at the 5.0% probability level and were found to be homogeneous. Animals were assigned to the study according to the following design. 20 Covance 6329-183 3M T-6295 Number of Animals Dietary Levels Group 1 (Control)a, b, c 2 (Low)b 3 (Mid)b 4 (Mid-High)b 5 (High)b, c 6 (High Recovery)d Male 70 60 60 60 70 40 Female 70 60 60 60 70 40 (ppm T-6295) 0 0.5 2.0 5.0 20.0 20.0 a The control animals received the control diet (basal diet with acetone). b Five animals/sex in Groups 1 through 5 were sacrificed at Weeks 4 and 14 for hepatocellular proliferation rate measurements, biochemical analyses (palmitoyl-CoA oxidation), and histopathology (Week 14 only). c Ten animals/sex in Groups 1 and 5 were designated as interim sacrifice animals. These animals were sacrificed after at least 52 weeks of treatment. d Animals in Group 6 were treated for at least 52 weeks, then treatment was discontinued, and the animals were observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 52 weeks posttreatment. During recovery, the animals received basal diet only. Dosing Procedures Dose Preparation. Dietary concentrations were based on the test material as supplied. Before initiation of treatment, diets of 0.5, 1, 2, and 20 ppm concentrations were prepared for stability and homogeneity analyses. Diets were prepared at least once every 4 weeks during the treatment phase of the study. Preparation of Diet for Group 1. The appropriate amount of certified diet was weighed and mixed with an appropriate volume of acetone. Preparation of Base Diet. A specified amount of certified diet was weighed into a labeled container. The required amount of test material was weighed and transferred into a labeled container and the appropriate volume of acetone was added to the container and mixed manually. Additional volume of acetone was added as necessary until the test material dissolved. To prepare a premix, the certified diet was transferred into a labeled Hobart mixing bowl. The test material and acetone were added to the mixing bowl, overlaid with a portion of diet from the mixing bowl, and thoroughly mixed. A portion of 21 Covance 6329-183 3M T-6295 diet from the mixing bowl was transferred to a second mixing bowl, mixed manually to recover residual test material, and returned to the first mixing bowl. The contents of the mixing bowl were thoroughly mixed. Preparation of Diets for Groups 2 through 4. Each dose level was prepared independently. A specified amount of certified diet was weighed into a labeled container and a specified amount of base diet was weighed for each dietary concentration. A pocket was formed in the feed in the mixing bowl and the amount of base diet was transferred to the pocket. The contents of the mixing bowl were thoroughly mixed for 15 minutes. Preparation of Diets for Groups 5 and 6. A specified amount of certified rodent diet (#5002) was weighed into a labeled Hobart mixing bowl and a specified amount of base diet was weighed for each diet. A pocket was formed in the feed in the mixing bowl and the amount of base diet was transferred to the pocket. The contents of the mixing bowl were thoroughly mixed for 15 minutes. Samples for dose analyses were taken directly from the mixing bowl. The diets were stored at room temperature in covered containers until dispensed into feeding jars. Method of Administration. Dietary admixture was used because the potential route of exposure in humans is oral. The dose preparations were administered ad libitum (see Protocol Deviations for exceptions). Animals in Groups 1, 2, 3 (males), 4, and 5 received the test diet for at least 104 weeks. Animals in Group 3 (females) received the test diet for at least 103 weeks. Animals in Group 6 received the test diet for 52 weeks. Retention Samples. Samples (approximately 100 g) were taken from each dose preparation during the inlife phase and stored at room temperature. These samples were discarded on June 15, 2000. 22 Covance 6329-183 3M T-6295 Dose Analysis Analyses for the concentration of test material in the dose preparations were done by Covance using an analytical method, MP-M383-MA, supplied by the sponsor and validated by Covance. Homogeneity was determined from the dose preparations mixed pretest and from preparations mixed for Week 1. One sample (approximately 100 g each) from the top, middle, and bottom of the dose preparations were collected, divided into three subsamples for extraction and analysis, and analyzed for test material content. All samples were stored at room temperature until analyzed within 7 days of mixing. To evaluate the stability of the test material in the carrier, one set of samples (approximately 100 g each) were taken from the preparations mixed pretest, stored at room temperature for 28 days, then analyzed. Homogeneity samples collected from the middle of the pretest dose preparations were analyzed within 2 days of mixing and used as the baseline value. An additional set of samples (approximately 100 g each) were taken from the preparations mixed for Week 1, stored at room temperature for 33 days, then analyzed. Homogeneity samples collected from the middle of the dose preparations for Week 1 were analyzed on the day of mixing and used as the baseline value. For dose confirmation, samples (approximately 100 g each) from all dose preparations were analyzed in duplicate. Homogeneity samples collected from the middle of the dose preparations for Week 1 were used for dose confirmation results. All samples were stored at room temperature until analyzed. Observation of Animals Clinical Observations. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity. Once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal was recorded. In addition, the time of onset, location, size, appearance, and progression of each grossly visible or palpable mass were recorded. Body Weights. Individual body weight data were recorded at the time of randomization, weekly for Weeks 1 through 17, once every 4 weeks thereafter, and at Week 105. 23 Covance 6329-183 3M T-6295 Food Consumption. Individual food consumption data were recorded weekly for Weeks 1 through 16 and once every 4 weeks thereafter. Administration of Bromodeoxyuridine. Seven days before the Week 53 interim sacrifice, osmotic pumps (ALZET Model 2ML1) were surgically implanted in five animals/sex/group from Groups 1 and 5. The osmotic pumps were preloaded with approximately 2 mL of bromodeoxyuridine (BrdU) at a concentration of 20 mg/mL. The animals were anesthetized by administration of acepromazine-ketamine-xylazine, and one pump/animal was aseptically inserted subcutaneously (dorsal surface). The incision was closed with wound clips, and the animals were monitored during clinical observations until the time of sacrifice to ensure that there were no clinical signs of infection. One Group 5 male implanted with the osmotic pump was sacrificed before the Week 53 sacrifice. Clinical Pathology Blood and urine samples were collected from 10 animals/sex/group in Groups 1 through 5 during Weeks 4, 14, 27, and 53. Blood films were made and held for possible future examination from animals sacrificed at unscheduled intervals and from animals at scheduled sacrifices after at least 52, 103 (Group 3 females), and 104 weeks of treatment. In addition, blood was collected for cholesterol and triglycerides from all remaining animals at the terminal and recovery sacrifices. Animals were fasted overnight, and urine was collected chilled overnight (approximately 16 hours) before blood sampling. Blood was collected from a jugular vein. The anticoagulant was potassium EDTA for hematology tests. The samples for clinical chemistry samples were collected without anticoagulant. Animals were bled in random order. The following were evaluated. red blood cell (erythrocyte) count hemoglobin hematocrit mean corpuscular volume mean corpuscular hemoglobin mean corpuscular hemoglobin concentration platelet count Hematology white blood cell (leukocyte) count differential blood cell count segmented neutrophil count lymphocyte count monocyte count eosinophil count basophil count blood cell morphology 24 Covance 6329-183 3M T-6295 Reticulocyte smears were made and held for possible future examination. glucose urea nitrogen creatinine total protein albumin globulin cholesterol total bilirubin appearance volume specific gravity pH protein urobilinogen sodium potassium Clinical Chemistry alanine aminotransferase gamma glutamyltransferase aspartate aminotransferase calcium inorganic phosphorus sodium potassium chloride Urinalysis glucose ketones bilirubin blood microscopic examination of sediment Urine Chemistry 16 hour excretion of: sodium potassium Serum PFOS Analyses During Weeks 4 and 14, blood samples (approximately 2 mL) were collected for serum analyses from five animals/sex/group in Groups 1 through 5 (animals selected for hepatocellular proliferation and biochemical analyses). During Week 53, blood samples (approximately 2 mL) were collected from five animals/sex/group in Groups 1 and 5 (from animals selected for interim sacrifice) and from all remaining animals at terminal and recovery sacrifices. Animals were fasted overnight. Blood was collected from a jugular vein, allowed to clot at room temperature, and centrifuged. Samples were collected without anticoagulant. Serum was harvested and stored in a freezer, set to maintain -60 to -80oC, until sent to the sponsor for analyses of PFOS levels and metabolites. Analysis of these samples is the responsibility of the sponsor; results are not included in this report. 25 Covance 6329-183 3M T-6295 Anatomic Pathology Necropsy at Unscheduled Intervals. A necropsy was done on each animal that died or was sacrificed at an unscheduled interval. Animals sacrificed at unscheduled intervals were anesthetized with carbon dioxide, weighed, and exsanguinated (see Protocol Deviations for exceptions). A blood film was taken as part of the necropsy procedure for sacrificed animals. Interim Sacrifices During Weeks 4, five animals/sex/group/interval in Groups 1 through 5 were fasted overnight, bled for serum chemistry, anesthetized with carbon dioxide, weighed, and exsanguinated. The abdominal cavity of each animal was opened, the liver was removed and weighed. Liver samples were collected for palmitoyl-CoA, cell proliferation, and PFOS concentration analyses and for mitochondrial activity. Animals sacrificed during Week 4 were discarded after liver collection. During Week 14, five animals/sex/group/interval in Groups 1 through 5 were fasted overnight, bled for serum chemistry, anesthetized with carbon dioxide, weighed, and exsanguinated. Liver samples were collected for palmitoyl-CoA, cell proliferation, PFOS concentration analyses, and for histopathology. During Week 53, 10 animals/sex/group from Groups 1 and 5 were fasted overnight, bled for serum samples (five animals/sex/group), anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. The liver was weighed and liver samples were collected and frozen for analysis. Animals sacrificed during Weeks 14 and 53 had macroscopic observations recorded, selected organs weighed, and selected tissues collected and preserved. A blood film was taken for each animal sacrificed at the Week 53 interim sacrifice. Animals were necropsied in random order. Terminal Sacrifice After at least 104 weeks of treatment (Week 103 for females in Group 3), the remaining animals were fasted overnight, bled for serum samples (five animals/sex/group), anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Animals were necropsied in random order. 26 Covance 6329-183 3M T-6295 Recovery Sacrifice After at least 52 weeks of treatment and 52 weeks without treatment, the remaining animals in Group 6 were fasted overnight, anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. A blood film was taken as part of the necropsy procedures at the terminal and recovery sacrifices. The necropsy included a macroscopic examination of the external features of the carcass; all external body orifices; the abdominal, thoracic, and cranial cavities; organs; and tissues. Cell Proliferation Tissue Collection and Immunohistochemical Evaluation. During the Week 4 and 14 tissue collection from animals in Groups 1 through 5 (five animals/sex/group), representative samples of the left lateral lobe and any macroscopic lesions of the liver were collected and preserved in zinc formalin. Liver samples were fixed and embedded in paraffin and blocks were sent to Pathology Associates International (PAI), PAI evaluated the samples (left lateral lobe only) for proliferation cell nuclear antigen (PCNA). In addition, liver sections were stained with hematoxylin and eosin and examined microscopically by PAI. The cell proliferation report is in Appendix 7. Palmitoyl-CoA (PCoA) Oxidase Analyses. During the Week 4 and 14 tissue collections from animals in Groups 1 through 5 (five animals/sex/group), a sample (approximately 500 mg) of the right lateral lobe of the liver was collected and flash-frozen in liquid nitrogen. The samples were stored in a freezer set to maintain -60 to -80oC, until analyzed by Covance for PCoA oxidase activity. Organ Weights. At the interim sacrifice during Weeks 14 and 53, the following organs (when present) were weighed; paired organs were weighed separately. adrenal (2) brain kidney (2) liver lung ovary (2) spleen testes thyroid (2) with parathyroid uterus with cervix 27 Covance 6329-183 3M T-6295 Organ-to-body weight percentages and organ-to-brain weight ratios were calculated. Liver PFOS Analysis. A portion of the liver was collected from five animals/sex/group from Groups 1 through 5 at the Week 4 and 14 interim sacrifices, from five animals/sex/group from Groups 1 and 5 at the Week 53 interim sacrifice, and from all remaining animals at the terminal and recovery sacrifice and flash-frozen in liquid nitrogen and stored in a freezer set to maintain -60 to -80oC, until packed on dry ice and shipped to the sponsor for PFOS and metabolites analyses. Analysis of these samples is the responsibility of the sponsor; results are not included in this report. Mitochondrial Analyses. During the Week 4 tissue collections for animals in Groups 1 through 5, liver samples (approximately 1.5 g) were collected and analyzed by the sponsor for mitochondrial activity. Analysis of these samples is the responsibility of the sponsor; results are not included in this report. Tissue Preservation. The following tissues were collected and preserved in 10% neutral-buffered formalin (see Protocol Deviations for exceptions). adrenal (2) brain cecum cervix colon duodenum epididymis (2) esophagus eye (2) femur with bone marrow (articular surface of the distal end) Harderian gland heart ileum jejunum kidney (2) lesions liver lung with mainstem bronchi lymph node (mesenteric) mammary gland (females only) ovary (2) pancreas pituitary prostate rectum salivary gland [mandibular (2)] sciatic nerve seminal vesicle (2) skeletal muscle (thigh) skin spinal cord (cervical, thoracic, and lumbar) spleen sternum with bone marrow stomach testis (2) thymus thyroid (2) with parathyroid trachea urinary bladder uterus vagina 28 Covance 6329-183 3M T-6295 Bone marrow smears from the femur of each animal at scheduled sacrifices were prepared, stained with Wright's stain, and retained for possible examination. In addition, at the terminal sacrifice, sections of the heart and liver were collected from 10 animals/sex in Groups 1, 4, 5, and 6 and preserved in 2.0% paraformaldehyde/2.5% glutaraldehyde in 0.1M phosphate buffer (see Protocol Deviations for exceptions). The tissues were processed and embedded in epoxy blocks for possible future examination by electron microscopy. Histopathology. Tissues (as appropriate) from each animal in Groups 1, 5, and 6 sacrificed at the Week 53 interim sacrifice were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Adrenals, brain, eyes, kidneys, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries from the animals necropsied at the Week 14 interim sacrifice were also embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Tissues (as appropriate) from each animal in Groups 1 and 5 sacrificed at the terminal sacrifice and any animals that died on test or were sacrificed at unscheduled intervals were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, mammary glands (females), and urinary bladder from each animal in Groups 2, 3, 4, and 6 sacrificed at the terminal and recovery sacrifices were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically (see Protocol Deviations for exceptions). Parathyroids were processed with the thyroids, but were not examined. At the Week 53 sacrifice, BrdU immunohistochemistry was performed by PAI on the livers and duodenums from five animals/sex/group in Groups 1 and 5 that received BrdU. In addition, sections of the liver and duodenums from these animals were stained with hematoxylin and eosin and examined microscopically. A pathology peer review was conducted by a pathologist designated by the sponsor. 29 Covance 6329-183 3M T-6295 Liver sections from five animals/sex/group from Groups 1 and 5 were stained with Oil Red "O" stain and examined microscopically. Liver sections in epoxy blocks for five animals/sex/group from Groups 1 and 5 were processed and evaluated by electron microscopy by Pathology Associates International (report included in Appendix 8). Slides of the pancreas from males found dead, sacrificed at an unscheduled interval, or sacrificed at the Week 14 and 53 interim sacrifices, terminal, and recovery sacrifices were sent to Tox Path Inc. for peer-review and results were provided to Covance for inclusion in the final report (see Protocol Deviations for exceptions). After examination, slides were returned to Covance. Statistical Evaluation Levene's test (Levene, 1960) was done to test for variance homogeneity. In the case of heterogeneity of variance at p < 0.05, transformations were used to stabilize the variance. One-way analysis of variance [ANOVA (Winer, 1971)] was used to analyze body weights, body weight changes, food consumption, continuous clinical pathology values, palmitoyl CoA oxidase activities, and organ weight data (Week14 and 53 interim sacrifices only). ANOVA was done on the homogeneous or transformed data. If the ANOVA was significant, Dunnett's t-test (Dunnett, 1964) was used for pairwise comparisons between treated and control groups. Group comparisons (Groups 2 through 6 versus Group 1) were evaluated at the 5.0%, two-tailed probability level. Only data collected on or after the first day of treatment were analyzed statistically. Survival and Tumor Analysis Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method using the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that were sacrificed at scheduled 30 Covance 6329-183 3M T-6295 intervals were censored in the analysis. Continuity-corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence in at least one treated group (Groups 2 through 5) was increased or decreased by at least two occurrences over the control group (Group 1). The high recovery group (Group 6) was selected based on the incidence rate (%) if it was increased or decreased by at least two as compared to the control or high-dose (20.0 ppm) group. When there was only one incidence in the high-recovery and none in the other groups, that case was excluded from the statistical evaluation. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level and are reported in Text Tables 3 and 4 for males and females, respectively. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses using the same criteria as in the case of nonneoplastic lesions. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but thequadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method using the death time or the first palpation time (as applicable) as a surrogate for the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question being the cause of death of a subset of the animals and the rest of the animals were assumed to be dead of other competing risks, IARC-type (Peto et al, 1980) cause of death analysis was performed. Specifically, the subset of the tumors which were assigned to be the cause of death by the study pathologist was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. Tumor types whose cause of death was undetermined, were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart et al. (1986). If one tumor in the group belonged to one of the two categories (fatal and incidental) in a test was combined with the other category for the purpose of statistical 31 Covance 6329-183 3M T-6295 analyses. In addition, in the cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the significance was obtained by using LogXact-Turbo (1993) and combined with the probability of Cox-Tarone test, if necessary. The benign and malignant neoplastic incidences were evaluated both separately and combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. One-sided trend and pairwise group comparisons were evaluated at the 5.0% significance level and are shown in Text Tables 5 and 6 for males and females, respectively. In the nonneoplastic analyses, animals sacrificed early (Week 4) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) were discounted from the number of animals at risk. In the neoplastic analyses, the low- (0.5 ppm), mid-(2.0 ppm), mid-high, and high recovery groups that did not have complete histopathology examinations were excluded from statistical analyses. In the text tables, the p-values under Group 1 (control) are for trend analysis and the p -values under Groups 2 (low), 3 (mid), 4 (mid-high), 5 (high) and 6 (high recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 5, and the p-values for those comparisons are indicated in the text tables. Trend analysis includes Groups 1, 2, 3, 4, and 5 only. Ordinal dose levels 0, 1, 2, 3, and 4 were used in all analyses for Groups 1, 2, 3, 4, and 5, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report designating direction and significance of effects. 32 + = Effect in the increased direction - = Effect in the decreased direction * = Significant at p < 0.05 ** = Significant at p < 0.01 E = Exact permutation test NA = Not analyzed Covance 6329-183 3M T-6295 Record Retention The raw data, documentation, records, specimens, protocol, and final report generated as a result of this study will be archived in the storage facilities of Covance-Madison. At least 1 year after submission of the final report, the Covance Archives staff will contact the sponsor. At that time, the sponsor may choose to have the aforementioned materials returned, archived for an additional period of time, or disposed of by Covance; a fee will be charged based on the archive disposition option selected by the sponsor. Raw data stored on magnetic media and the protocol, study correspondence, and the original final report will be retained by Covance. At least 1 year after submission of the final report, any frozen samples will be returned to the sponsor, retained by Covance, or disposed of, and a fee will be charged. Data generated by the sponsor will be archived in the sponsor's storage facilities. Specimens, data, and reports for PCNA evaluation, BrdU immunohistochemistry and electron microscopy will be retained by PAI. RESULTS AND DISCUSSION Dose Analysis Results of the homogeneity, stability, and dose preparation analyses are in Tables 1 through 3. Several homogeneity analyses were conducted on each of the several mixes in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity in the analytical method for measuring Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295). Mean values of the homogeneity analyses for the pre-dosing mix done on March 18, 1998, ranged from 119-135% and 107-118% of the theoretical concentrations for the diets containing 0.5 and 20 ppm (PFOS; T-6295), respectively. Mean values of 33 Covance 6329-183 3M T-6295 the homogeneity analyses for the mix done on April 16, 1998, ranged from 96.2-122%, 98.5-107%, 97.6-101%, and 90.0-101% of the theoretical concentrations for the diet containing 0.5, 2, 5, and 20 ppm (PFOS; T-6295), respectively. Mean values of the homogeneity analyses for the mix done on April 22, 1998, ranged from 93.5-103% of the theoretical concentrations for the diet containing 20 ppm (PFOS; T-6295). Mean values of the homogeneity analyses for the mix done on June 18, 1998, ranged from 91.0-175%, 111-130%, 99.6-102%, and 90.5-97.0% of the theoretical concentrations for the diet containing 0.5, 2, 5, and 20 ppm (PFOS; T-6295). These results indicate that the mixing procedure produced a homogeneous distribution of the test material in the dose preparations; although variability generally appeared slightly greater at the lower dietary concentrations. Stability analyses were conducted prestudy in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity at levels in the analytical method for measuring (PFOS; T-6295). Results of stability analyses for the pre-dosing mix conducted on March 18, 1998, of samples stored for 28 days at room temperature indicated that the mean concentrations were 78.5, 76.7, 79.8, and 88.1% of the initial concentrations of 0.5, 1, 2, and 20 ppm, respectively. Results of stability analyses for the mix conducted on April 16, 1998, of samples stored for 33 days at room temperature indicated that the mean concentrations were 112.3, 119.6, 113.9, and 126.7% of the initial concentrations of 0.5, 2, 5, and 20 ppm, respectively. The mean concentrations of the dose preparation analyses for all levels ranged from 44.4-635% of the theoretical concentrations (including the reassay and retention sample analyses). The overall mean concentrations within each dose level ranged from 102.3 to 108.7. Inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring (PFOS; T-6295) resulted in homogeneity, stability, and routine analysis data that were in many cases outside of the standard limits of +/- 15%. Observation of Animals Survival and Clinical Observations. Clinical observations including mass observations are summarized in Tables 5 and 6; individual data are in Appendix 2. Adjusted survival data are illustrated in Figures 1 and 2 and summarized in Table 4; individual animal fate data are in Appendix 2. 34 Covance 6329-183 3M T-6295 Survival after 104 weeks of treatment was 22.0, 22.0, 34.0, 50.0, 45.0, and 27.0% for males in Groups 1, 2, 3, 4, 5, and 6, respectively, and 50.0, 30.0, 20.0 (after 102 weeks), 34.0, 52.0, and 48.0% for females in Groups 1, 2, 3, 4, 5, and 6, respectively. There was a significant increased trend in survival that occurred in the males that and was due to significant increases in survival in mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control group. None of the other treated groups in the males revealed any significant differences in survival. No significant trend was noted in survival in females. There was a significant decrease in survival in the mid-dose (2.0 ppm) group and not in the mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control. There were no clinical observations attributed to administration of the test material. The types of clinical observations noted are commonly observed in laboratory rats as they age on long term safety studies. There was no effect of the test material on the incidence of palpable masses. Body Weights. Body weight and body weight change data are illustrated in Figures 3 and 4 and summarized in Tables 7 and 8; individual body weight data are in Appendix 3. Males given 20 ppm (Groups 5 and 6) had significantly lower mean body weights compared to animals in the control group during Weeks 9 through 37. During recovery the mean body weights for Group 6 males were similar to mean body weights for Group 5 males. Females in Group 5 given 20 ppm had significantly lower body weights compared to animals in the control group during Weeks 3 through 101. Mean body weights for females in Group 6 given 20 ppm were statistically lower than mean body weights for control females during Weeks 3 through 61, but their mean body weights became nearer to the weights of the control females when placed on recovery. At Week 105, mean body weights for surviving males were 98, 108, 103, 101, and 96% of controls for the low-, mid-, and high-dose groups (Groups 5 and 6), respectively. At Week 105, mean body weights for surviving females were 101, 107 (Week 101), 100, 86, and 105% of controls, for the low-, mid-, and high-dose groups (Groups 5 and 6), respectively. Overall mean body weight change was statistically significantly lower for males given 20 ppm diets for Weeks 1 through 15 and Weeks 15 through 29. Mean body weight change was statistically significantly lower for females given 20 ppm for Weeks 1 35 Covance 6329-183 3M T-6295 through 15 and Weeks 29 through 57 (Group 5 only). Mean body weight changes for males and females in Group 6 were significantly increased during the several weeks of recovery. Animals at lower dose levels occasionally had statistically significant decreases in body weight gain; however, these occurrences were inconsistent over time between sexes and were not clearly dose-related. Food Consumption. Food consumption data are illustrated in Figures 5 and 6 and summarized in Table 9; individual data are in Appendix 4. Although not always statistically significant, males given 20 ppm tended to consume less food during Weeks 1 through 24. Food consumption was similar for males given 20 ppm compared to animals given the control material during Weeks 28 through 104. Statistically significantly lower food consumption was noted for females given 20 ppm during Weeks 2 through 36. Food consumption for males and females was similar in all the other treated groups compared to animals given the control material. Test Material Consumption. Test material consumption data are summarized in Table 10. Individual test material consumption data are presented in Appendix 4. Animals were fed diets containing 0, 0.5, 2, 5, or 20 ppm T-6295. The amount of test material consumed by animals on a mg/kg of body weight/day basis was as follows. Group 2 3 4 5 6 Dietary Concentrations (ppm) 0.5 2.0 5.0 20.0 20.0 Mean Achieved Dose Levels - Range (mg/kg body weight/day) Males Females 0.015 - 0.057 0.015 - 0.052 0.064 - 0.226 0.073 - 0.213 0.153 - 0.570 0.186 - 0.559 0.643 - 2.205 0.838 - 2.149 0.732- 2.336 1.047 - 2.160 Clinical Pathology Hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry data are summarized in Tables 11 through 29; individual data are in Appendix 5. The Pathology Report contains a discussion of the data. 36 Covance 6329-183 3M T-6295 Dietary administration of PFOS for approximately 53 weeks was associated with mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. None of these effects were considered adverse. The effect on cholesterol was no longer apparent after 104 weeks of treatment. There was no effect on hepatic palmitoyl-CoA oxidase activity. Anatomic Pathology Organ weight data are summarized in Tables 30 through 31; incidences of macroscopic and microscopic observations are summarized in Tables 32 through 41. Individual data are in Appendix 6. The Pathology Report contains a discussion of the data. Terminal body weights in males and females at Week 14 were comparable between control and treated groups. At the Week 14 interim sacrifice, absolute and relative liver weights were significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas in males and females, and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. At the Week 53 interim sacrifice, terminal body weights were significantly decreased in the females given 20 ppm. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. At the Week 53 interim sacrifice, centrilobular hepatocytic hypertrophy and vacuolation was increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated 37 Covance 6329-183 3M T-6295 with the administration of the test material. Liver findings in several unscheduled deaths given 20 ppm resembled those seen in animals sacrificed at Week 53. At the terminal sacrifice, the livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in the animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. In the unscheduled sacrifices between Weeks 54 and 105, animals given 20 ppm had increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, and centrilobular hepatocytic pigment were noted. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. A significant positive trend was noted in the incidences of liver hepatocellular adenoma for males. This was associated with a significant increase in the high-dose (20.0 ppm) group over the control. The high-dose recovery (20.0 ppm) group animals in this case exhibited a significantly decreased incidence when compared to that of the high-dose (20.0 ppm) group. Significantly increased incidences were observed for thyroid follicular cell adenoma in the high-dose recovery (20.0 ppm) group when compared to either the control or the high-dose (20.0 ppm) group. There was also a significant increase in the combined (adenoma and carcinoma) thyroid follicular cell tumors in the high-dose recovery (20.0 ppm) group animals as compared to that of the high-dose (20.0 ppm) group. None of these cases were associated with any significant trend. Thyroid follicular cell carcinoma did not show any significant effect. There were no other significant neoplastic findings in the males. Significant positive trends were observed in the incidences of hepatocellular adenoma and hepatocellular combined adenoma and carcinoma in the females. These cases were associated with significant increases in the high-dose (20.0 ppm) group as compared to the control. All these tumors were incidental and noted at the terminal sacrifice. A 38 Covance 6329-183 3M T-6295 marginally significant increase for thyroid follicular cell adenoma and carcinoma combined was observed in the mid-high (5.0 ppm) group but not in the high-dose (20.0 ppm) group as compared to the control group. In fact, thyroid C-cell adenoma and thyroid C-cell combined adenoma and carcinoma actually showed significant decreases in the high-dose (20.0 ppm) group as compared to the control. There were significant negative trends in the incidences of mammary fibroadenoma and mammary combined adenoma and fibroadenoma. These cases were associated with significantly lower incidences in the respective high-dose (20.0 ppm) group when compared to that of the control. However, the low-dose (0.5 ppm) group animals for these cases exhibited significant increases over the control. A significant decreased incidence was found for the high-dose recovery (20.0 ppm) group in the incidences of mammary carcinoma as compared to the high-dose (20.0 ppm) group. There was no other significant group effect in mammary carcinoma. There was a marginal significant negative trend for the mammary combined fibroadenoma/adenoma/carcinoma. However, the low- (0.5 ppm) and mid-high (5.0 ppm) groups for this case exhibited significantly increased incidences over the control. There were no other significant neoplastic findings in the females. Cell Proliferation Results There were no statistically significant increases in cell proliferation as measured by PCNA at weeks 4 and 14, or by bromodeoxyuridine (BrdU) immunohistochemistry at week 53. However, two out of five females in each of the mid-high and high dose groups showed a very mild, but biologically significant response (i.e. had a proliferative index that was twice the mean of the controls and greater than that of the highest control) at week 4. The cell proliferation response at week four was transient in nature as it was not observed at week 14, and was considered to be test compound-related. Electron Microscopy Results Liver samples were obtained from each of the 20 rats during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for ultrastructural changes that may be related to chronic toxicity of the test material when administered in the diet. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic 39 Covance 6329-183 3M T-6295 reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the high dose group and the control animals. Other changes included a slight increase in the amount of glycogen in treated animals compared with controls. Characterization of Tumor Incidence Outcome In males, hepatocellular adenoma was increased for trend (p = 0.0276) and pair-wise against control for the 20 ppm dose group (7/60 20 ppm vs. 0/60 control, p = 0.0456). Hepatocellular adenoma was not observed in the 20 ppm recovery group, which was significant compared to the 20 ppm dose group (0/40 20 ppm recovery group vs. 7/60 200 ppm dose group, p = 0.0240). Overall, the response in terms of hepatocellular adenoma can be considered weak at the 20 ppm dose level. Thyroid follicular cell adenoma was increased pair-wise against control for the 20 ppm recovery group (9/39 20 ppm recovery group vs. 3/60 control, p = 0.0280). Although this finding is outside the range of recent historical control values in this laboratory, there were no other indications of thyroid abnormality. In females, hepatocellular adenoma was increased for trend (p = 0.0153) and pair-wise against control for the 20 ppm dose group (5/60 20 ppm dose group vs. 0/60 control, p = 0.0386). Overall, the response in terms of hepatocellular adenoma can be considered weak at the 20 ppm dose level. The only hepatocellular carcinoma observed in the study was at the high dose. Hepatocellular adenoma/carcinoma was increased for trend (p = 0.0057) and pair-wise against control for the 20 ppm dose group (6/60 20 ppm vs. 0/60 control, p = 0.0204). Mammary fibroadenoma was decreased for trend (p = 0.0152) and pair-wise against control for the 20 ppm dose group (11/60 20 ppm vs. 20/60 control, p = 0.0235). 40 CONCLUSION Covance 6329-183 3M T-6295 The chronic toxicity and carcinogenicity of the test material, Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) was assessed following dietary admixture to male and female Crl:CD(SD)IGS BR rats at dose levels of 0.5, 2, 5, or 20 ppm. Based on anatomic pathology findings in the liver, the no-observable-adverse-effect level for Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) is considered to be 2 ppm. 41 SIGNATURES Covance 6329-183 3M T-6295 42 Covance 6329-183 3M T-6295 REFERENCES Dunnett, C. W., "New Tables for Multiple Comparisons with a Control," Biometrics, 20:482-491 (1964). Levene, H., "Robust Tests for Equality of Variances," Contributions to Probability and Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960). Winer, B. J., "Design and Analysis of Single-Factor Experiments," Statistical Principles in Experimental Design, Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971a). Winer, B. J., "Analysis of Covariance," Statistical Principles in Experimental Design, Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971b). 43 PATHOLOGY REPORT Covance 6329-183 3M T-6295 SUMMARY The purpose of this study was to assess the chronic toxicity and carcinogenicity of the test material, Perfluorooctane Sulfuric Acid Potassium Salt (PFOS) when administered in the diet to rats for at least 104 weeks. The test material was administered at dose levels of 0.5, 2, 5, and 20 ppm. Dietary administration of PFOS for approximately 53 weeks was associated with mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. None of these effects were considered adverse. The effect on cholesterol was no longer apparent after 104 weeks of treatment. There was no effect on hepatic palmitoyl-CoA oxidase activity. Terminal body weights in males and females at Week 14 were comparable between control and treated groups. At the Week 14 interim sacrifice, absolute and relative liver weights were significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas in males and females, and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. At the Week 53 interim sacrifice, terminal body weights were significantly decreased in the females given 20 ppm. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. At the Week 53 interim sacrifice, centrilobular hepatocytic hypertrophy and vacuolation was increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular 44 Covance 6329-183 3M T-6295 hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated with the administration of the test material. Liver findings in several unscheduled deaths given 20 ppm resembled those seen in animals sacrificed at Week 53. At the terminal sacrifice, the livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in the animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. In the unscheduled sacrifices between Weeks 54 and 105, animals given 20 ppm had increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, and centrilobular hepatocytic pigment were noted. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. A complete discussion of statistically significant differences in the incidence of neoplasms may be found in the section entitles "Statistical Report of Survival, Nonneoplastic, and Neoplastic Lesions." METHODS Six groups of Crl:CD(SD)IGS BR rats were studied using the following study design. 45 Group 1 (Control) 2 (Low) 3 (Mid) 4 (Mid-High) 5 (High) 6 (High Recovery) Number of Animals Male Female 70 70 60 60 60 60 60 60 70 70 40 40 Covance 6329-183 3M T-6295 Dietary Levels (ppm T-6295) 0 0.5 2.0 5.0 20.0 20.0 The animals in Group 6 were designated as recovery animals and were treated for 52 weeks, then placed in recovery for an additional 52 weeks. During Weeks 4, 14, 27, and 53, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from 10 animals/sex in Groups 1 through 5. Blood was collected for cholesterol and triglyceride determinations from all animals prior to the terminal sacrifice during Weeks 105 and 106 (Week 103 for females in Group 3) and the recovery sacrifice (Group 6) during Weeks 105 and 106. Blood films were also prepared for animals at the terminal and recovery sacrifices. Five animals/sex in Groups 1 through 5 were sacrificed during Week 4; livers were collected and weighed. A portion of the liver was shipped to the sponsor for PFOS analysis and mitochondrial activity, a portion was shipped to Pathology Associates International (PAI) for hepatocellular proliferation rate measurements by proliferation cell nuclear antigen (PCNA), and a third portion was used for determination of palmitoyl-CoA oxidase activity. During Weeks 14 and 53, necropsies were performed on five and 10 animals/sex, respectively, in Groups 1 through 5. Surviving females in Group 3 were sacrificed and necropsied during Week 103 because of reduced survival. The remaining animals in Groups 1 through 6 were sacrificed and necropsied during Weeks 105 and 106. At necropsy, macroscopic observations were recorded, organ weights were obtained (Weeks 14 and 53), and tissues were placed in fixative as specified by the protocol. In addition, liver samples were collected for PFOS analysis (Weeks 14, 53, 103, 105, and 106), hepatocellular proliferation rate measurement [by PCNA at Week 14 and by bromodeoxyuridine (BrdU) immunohistochemistry at Week 53], and palmitoyl-CoA oxidase determination (Week 14). A section of duodenum was also collected at Week 53 for BrdU immunohistochemistry. At the terminal and recovery sacrifices, sections of heart and liver from 10 animals/sex in Groups 1, 4, 5, and 6 were preserved in 2.0% parafomaldehyde/2.5% gluteraldehyde in 0.1M phosphate buffer, processed, and embedded in epoxy resin for possible future electron microscopy. 46 Covance 6329-183 3M T-6295 At unscheduled necropsies for animals that died or were sacrificed because of poor health, macroscopic observations were recorded, and tissues were placed in fixative, but organ weights were not obtained. Microscopic examinations were done on selected tissues (adrenals, brain, eyes, kidney, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries) from the animals necropsied during Week 14 and on all tissues from the animals in Groups 1 and 5 that were necropsied during Weeks 53, 105, and 106. In addition, microscopic examinations were done on the tissues from animals that died or were sacrificed due to poor health. Macroscopic lesions, liver, lung, kidneys, pancreas, thyroid, testes, and mammary gland (females) were examined for animals in Groups 2, 3, 4, and 6 that were sacrificed during Weeks 103, 105, and 106. Sections of liver were stained with Oil Red O and examined from five terminally sacrificed males and females in Groups 1 and 5. Also, urinary bladder was examined microscopically from all terminally sacrificed animals in Groups 2, 3, and 4, and from all animals at the recovery sacrifice. Statistically significant differences cited in the Results and Discussion section are based on comparisons between the control and treated groups. RESULTS AND DISCUSSION Clinical Pathology Weeks 4, 14, 27, and 53. There were relatively few statistically significant or otherwise notable differences for clinical pathology results between the control and treated groups. Those differences that were the most consistent over time were considered to be associated with administration of the test material. These included mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. There were no correlative microscopic renal findings for the minor change in urea nitrogen. The findings for alanine aminotransferase, and possibly for glucose and cholesterol, were likely associated with the histopathological findings of hepatocellular hypertrophy and vacuolation. None of the clinical pathology effects were considered adverse. 47 Covance 6329-183 3M T-6295 Of uncertain relationship to the test material were statistically higher urea nitrogen for males fed 2 ppm and statistically lower glucose for females fed 2 or 5 ppm at Week 53. These findings were not as consistent over time as those considered to be effects of the test material, and the magnitudes of these differences were very small. All other statistically significant differences for clinical pathology results between the control and treated groups were considered incidental. Most of these differences did not affect animals fed the highest dose level, and none of these differences were consistent over time. Week 103 (Day 719; Group 3 Females) and Week 105. As expected for rats of this age, the cholesterol and triglyceride results were extremely variable, especially for the males. Because of this naturally-occurring variability, the effect on cholesterol observed at earlier intervals was no longer clearly apparent. Although males and females fed 20 ppm had lower mean cholesterol concentrations than those of the other groups, the marked individual variability made it impossible to definitively identify a test material-related effect on cholesterol at Week 105. The recovery males (Group 6) had a mean cholesterol concentration similar to that of the control males, but the recovery females had a mean cholesterol concentration that was similar to the females in Group 5. Once again, the marked individual variability for cholesterol obscured the significance of these findings. Anatomical Pathology Week 14, Terminal Body Weight and Organ Weights. Terminal body weights were comparable between control and treated groups. Absolute and relative liver weights were statistically significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. The absolute spleen weight was significantly decreased in the females given 20 ppm, as was the absolute lung weight in females given 2, 5, or 20 ppm. Spurious, significant increases in left thyroid/parathyroid-to-body weight percentages were seen in females given 5 or 20 ppm. Macroscopic Observations. There were no macroscopic observations that could be attributed to the administration of the test material. 48 Covance 6329-183 3M T-6295 Microscopic Observations. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. There were no potentially test material-related lesions in the remaining tissues examined. Week 53 Interim Sacrifice, Body and Organ Weights. In the females, terminal body weights were significantly decreased in the group given 20 ppm. Terminal body weights in the males were comparable between the control and treated group. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. Significantly decreased left thyroid/parathyroid weights were considered to be spurious due to the absence of a contralateral effect. In view of the significant decrease in body weight for females given 20 ppm, significant increases in organ-to-body weight percentages for brain, kidney, liver, and spleen may be of no toxicological importance. Decreased absolute weights in the left adrenal gland and bilateral adrenal-to-brain weight ratios may also represent changes secondary to the body weight loss. Macroscopic Observations. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. Microscopic Observations. Centrilobular hepatocytic hypertrophy and vacuolation were increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated with the administration of the test material. 49 Covance 6329-183 3M T-6295 Unscheduled Deaths Through Week 53 Large, mottled, or diffusely dark livers were noted in 2/3 males and 1/1 females given 20 ppm. There were no other gross observations that could be attributed to the administration of the test material. Microscopically, several animals given 20 ppm exhibited liver changes that were similar or related to those seen at the Week 53 interim sacrifice. Terminal Sacrifice Macroscopic Observations. The livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Microscopic Observations. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. There were no clear differences in the Oil Red O stained sections between Groups 1 and 5. Recovery Sacrifice Macroscopic Observations. There were no consistent gross findings in the recovery sacrifice animals that could be attributed to the test material. Microscopic Observations. The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. Unscheduled Deaths Weeks 54-105 Macroscopic Observations. There were no consistent gross findings in the recovery sacrifice animals that could be attributed to the test material. Microscopic Observations. Liver findings of the died-on-test or moribund sacrifice animals were generally similar to those seen in the terminally-sacrificed animals. Increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, 50 Covance 6329-183 3M T-6295 and centrilobular hepatocytic pigment were noted in the animals given 20 ppm. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. Neoplasm Analysis - All Animals A complete discussion of statistically significant differences in the incidence of neoplasms may be found in the section entitles "Statistical Report of Survival, Nonneoplastic, and Neoplastic Lesions." 51 Covance 6329-183 3M T-6295 52 Covance 6329-183 3M T-6295 53 Covance 6329-183 3M T-6295 54 Covance 6329-183 3M T-6295 55 Covance 6329-183 3M T-6295 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC, AND NEOPLASTIC LESIONS Methods Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method using the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that were sacrificed at scheduled intervals were censored in the analysis. Continuity-corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level, as reported in Text Tables 1 and 2. Kaplan-Meier product limit survival curves are presented in Text Figures 1 and 2 for males and females, respectively. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence in at least one treated group (Groups 2 through 5) was increased or decreased by at least two occurrences over the control group (Group 1). The high recovery group (Group 6) was selected based on the incidence rate (%) if it was increased or decreased by at least two as compared to the control or high-dose (20.0 ppm) group. When there was only one incidence in the high-recovery and none in the other groups, that case was excluded from the statistical evaluation. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level and are reported in Text Tables 3 and 4 for males and females, respectively. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses using the same criteria as in the case of nonneoplastic lesions. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but the quadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method using the death time or the first palpation time (as applicable) as a surrogate for the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question being the cause of death of a subset of the animals and the rest of the animals were assumed to be dead of other competing risks, IARC-type (Peto et al, 56 Covance 6329-183 3M T-6295 1980) cause of death analysis was performed. Specifically, the subset of the tumors which were assigned to be the cause of death by the study pathologist was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. Tumor types whose cause of death was undetermined, were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart et al. (1986). If one tumor in the group belonged to one of the two categories (fatal and incidental) in a test was combined with the other category for the purpose of statistical analyses. In addition, in the cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the significance was obtained by using LogXact-Turbo (1993) and combined with the probability of Cox-Tarone test, if necessary. The benign and malignant neoplastic incidences were evaluated both separately and combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. One-sided trend and pairwise group comparisons were evaluated at the 5.0% significance level and are shown in Text Tables 5 and 6 for males and females, respectively. In the nonneoplastic analyses, animals sacrificed early (Week 4) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) were discounted from the number of animals at risk. In the neoplastic analyses, the low- (0.5 ppm), mid-(2.0 ppm), mid-high (5.0 ppm), and high recovery (20 ppm) groups that did not have complete histopathology examinations were excluded from statistical analyses. In the text tables, the p-values under Group 1 (control) are for trend analysis and the p -values under Groups 2 (low), 3 (mid), 4 (mid-high), 5 (high) and 6 (high recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 5, and the p-values for those comparisons are indicated in the text tables. 57 Covance 6329-183 3M T-6295 Trend analysis includes Groups 1, 2, 3, 4, and 5 only. Ordinal dose levels 0, 1, 2, 3, and 4 were used in all analyses for Groups 1, 2, 3, 4, and 5, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report designating direction and significance of effects. + = Effect in the increased direction - = Effect in the decreased direction * = Significant at p < 0.05 ** = Significant at p < 0.01 E = Exact permutation test NA = Not analyzed Results and Discussion Survival. As indicated in Text Table 1, a significant negative trend (p = 0.0005) for decreased mortality occurred in for the males and was associated with significant decreases in mortality in mid-high- (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control group (p = 0.0115 and 0.0292, respectively). None of the other treated groups in the males revealed any significant differences in mortality. As indicated in Text Table 2, no significant trend was noted in mortalilty in females. There was a significant increase in mortality in the mid-dose (2.0 ppm) group (p = 0.0015) and not in the mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control. Nonneoplastic Lesions. The liver, pancreas, and thyroid in both sexes have been identified as appropriate to perform statistical analysis for nonneoplastic lesions. As indicated in Text Table 3, there were significant positive trends in the incidences of hepatocellular centrilobular hypertrophy, liver eosinophilic centrilobular granular cytoplasm, and hepatocellular midzonal/centrilobular vacuolation (p < 0.01 for all the cases) for males. These cases were associated with significant increases in the high-dose (20.0 ppm) group (p < 0.01 for all the cases) when compared to those of the control group. In addition, there were significantly increased incidences in the mid- (2.0 ppm) and mid-high-dose (5.0 ppm) groups for hepatocellular centrilobular hypertrophy 58 Covance 6329-183 3M T-6295 (p = 0.0415 and 0.0000, respectively) and the mid-high-dose (5.0 ppm) group for hepatocellular midzonal/centrilobular vacuolation (p = 0.0024) as compared to the control in this sex. However, the male high-dose recovery (20.0 ppm) group animals in all these cases exhibited significantly decreased incidences over the high-dose (20.0 ppm) group (p < 0.01 for all the cases), implying that the effect may be transient (reversible) in nature. Significant positive trends were also noted for hepatocellular centrilobular pigment and liver individual hepatocyte necrosis (p = 0.0006 and 0.0106, respectively) in association with significant increases in the respective high-dose (20.0 ppm) group over the control (p = 0.0139 and 0.0224, respectively). A significant positive trend was detected for cystic degeneration in association with significantly increased incidences in all the dose groups as compared to the control (p < 0.01 for Groups 2 through 6 vs. 1, respectively ). Furthermore, a significant positive trend without any significant group effect was noted for liver degeneration/necrosis centrilobular (p = 0.0132). Marginally significant increases over the control were observed in the mid-high-dose (5.0 ppm) group incidences for hepatocellular altered basophilic focus and liver coagulative necrosis (p = 0.0352 and 0.0351, respectively) and in the mid-dose (2.0 ppm) group for pancreatic interstitial fat infiltration (p = 0.0133) without any significant trends. As indicated in Text Table 4, there were significant positive trends in the incidences of hepatocellular centrilobular hypertrophy and liver eosinophilic centrilobular granular cytoplasm (p < 0.01 for both cases) for the females. These cases were associated with significant increases in respective mid-high (5.0 ppm) and high-dose (20.0 ppm) groups (p < 0.01 for all the cases) when compared to that of the control group. Significant positive trends were also noted for hepatocellular centrilobular pigment and liver individual hepatocyte necrosis (p = 0.0000 and 0.0359, respectively). These cases were associated with significant increases in the respective high-dose (20.0 ppm) group (p = 0.0000 and 0.0500, respectively) when compared to that of the control group. However, the high-dose recovery (20.0 ppm) group animals in all the above cases exhibited significantly decreased incidences over the high-dose (20.0 ppm) group (p < 0.01 for all the cases, except for liver individual hepatocyte necrosis (p = 0.0329) implying that the effect may be transient in nature. A significant positive trend in association with a significant increase in the high-dose (20.0 ppm) group was found for lymphohistiocytic infiltration in the liver (p = 0.0080 for trend and 0.0038 for high-dose versus control). A significant positive trend was also noted for cystic degeneration in the liver (p = 0.0187). However, no significant group comparison was detected. In addition, there was a significant positive trend in the incidences of pigmented macrophage 59 Covance 6329-183 3M T-6295 infiltration in the liver (p = 0.0000). This was associated with significant increases in the high-dose (20.0 ppm) and high-dose recovery (20.0 ppm) groups as compared to the control (p = 0.0000 and 0.0147, respectively). However, the high-dose recovery (20.0 ppm) group animals exhibited significantly decreased incidences over the high-dose (20.0 ppm) group (p = 0.0383). A marginally significant positive trend was noted for Zymogen decreased granules in the pancreas (p = 0.0488). This was associated with significant increases in all dosed groups (p = 0.0093, 0.0011, 0.0329, 0.0127, and 0.0030 for Group 2 through 6 versus 1, respectively) when compared to the control. Significant increases were also observed over the control in the mid-dose (2.0 ppm) incidences of hepatocellular periportal vacuolation (p = 0.0358) and the low-dose (0.5 ppm) incidences of liver hematopoiesis extramedullary (p = 0.0.0352). However, no significant trends were associated with them. A significant negative trend was observed for hepatocellular hypertrophy periportal (p = 0.0026). This was associated with a significant decrease in the high-dose (20.0 ppm) group (p = 0.0127) as compared to that of the control The high-dose recovery (20.0 ppm) animals in this case exhibited significantly increased incidences (p = 0.0344) when compared to the high-dose (20.0 ppm) group. Significant negative trends were also noted for angiectasis and congestion in the liver (p = 0.0231 and 0.0451, respectively) and islet cell hyperplasia in the pancreas (p=0.0372). However, no significant group comparisons were found for these cases. Neoplastic Lesions. As indicated in Text Table 5, a significant positive trend was noted in the incidences of liver hepatocellular adenoma for males (p = 0.0276). This was associated with a significant increase in the high-dose (20.0 ppm) group over the control (p = 0.0456). The high-dose recovery (20.0 ppm) group animals in this case exhibited a significantly decreased incidence when compared to that of the high-dose (20.0 ppm) group (p = 0.0240). Significantly increased incidences were observed for thyroid follicular cell adenoma in the high-dose recovery (20.0 ppm) group when compared to either the control or the high-dose (20.0 ppm) group (p= 0.0280 and 0.0121, respectively). There was also a significant increase in the combined (adenoma and carcinoma) thyroid follicular cell tumors in the high-dose recovery (20.0 ppm) group animals as compared to that of the high-dose (20.0 ppm) group (p = 0.0125). None of these cases were associated with any significant trend. Thyroid follicular cell carcinoma did not show any significant effect. There were no other significant neoplastic findings in the males. 60 Covance 6329-183 3M T-6295 As indicated in Text Table 6, significant positive trends were observed in the incidences of hepatocellular adenoma (p = 0.0153) and hepatocellular combined adenoma and carcinoma (p = 0.0057) in the females. These cases were associated with significant increases in the high-dose (20.0 ppm) group as compared to the control (p < 0.05 for both cases). All these tumors were incidental and noted at the terminal sacrifice. A marginally significant increase for thyroid follicular cell adenoma and carcinoma combined was observed in the mid-high (5.0 ppm) group (p = 0.0471) but not in the high-dose (20.0 ppm) group as compared to the control group. In fact, thyroid C-cell adenoma and thyroid C-cell combined adenoma and carcinoma actually showed significant decreases in the high-dose (20.0 ppm) group as compared to the control (p = 0.0336 for both cases). There were significant negative trends in the incidences of mammary fibroadenoma and mammary combined adenoma and fibroadenoma (p 0.05 for both cases). These cases were associated with significantly lower incidences in the respective high-dose (20.0 ppm) group (p < 0.05 for both cases) when compared to that of the control. However, the low-dose (0.5 ppm) group animals for these cases exhibited significant increases over the control (p < 0.05 for both cases). A significant decreased incidence was found for the high-dose recovery (20.0 ppm) group in the incidences of mammary carcinoma (p = 0.0161) as compared to the high-dose (20.0 ppm) group. There was no other significant group effect in mammary carcinoma. There was a marginal significant negative trend for the mammary combined fibroadenoma/adenoma/carcinoma (p = 0.0482). However, the low- (0.5 ppm) and mid-high (5.0 ppm) groups for this case exhibited significantly increased incidences over the control (p = 0.0474 and 0.0066, respectively). There were no other significant neoplastic findings in the females. References Dinse, G.E. and Lagakos, S.W., "Regression analysis of tumor prevalence data," Journal of Royal Statistical Society Series C (Applied Statistics), 32: 236-248 (1983). Gart, J.J., Krewski, D., Lee, P.N., Tarone, R.E., and Wahrendorf, J., Statistical Methods in Cancer Research: The Design and Analysis of Long-Term Animal Experiments, IARC Scientific Publications, Vol. 3, No. 79, Oxford University Press, New York (1986). LogXact-Turbo, Cytel Software Corporation, Version 1.1, Cambridge, Massachusetts, 1993. 61 Covance 6329-183 3M T-6295 McConnell, E.E., Solleveld, H.A., Swenberg, J.A., and Boorman, G.A., "Guidelines for combining neoplasms for evaluation of rodent carcinogenicity studies," Journal of National Cancer Institute, 76:283-289 (1986). Peto, R., Pike, M.C., Day, N.E., Gray, R.G., Lee, P.N., Parish, S., Peto, J., Richards, S., and Wahrendorf, J., "Guidelines for simple, sensitive significance tests for carcinogenic effects in long-term animal experiments, in Long-term and Short-term Screening Assays for Carcinogens: A Critical Appraisal," Lyon: International Agency for Cancer Research (IARC), 311-426 (1980). Thakur, A.K., K.J. Berry, and Mielke, P.W., Jr., "A FORTRAN program for testing trend and homogeneity in proportions," Computer Programs in Biomedicine, 19: 229-233 (1985). Thomas, D.G., Breslow, N., and Gart, J. J., "Trend and homogeneity analyses of proportions and life table data," Computers and Biomedical Research, 10:373-381 (1977). 62 Covance 6329-183 3M T-6295 63 Text Table 1 Results of Statistical Analyses of Survival Data for Male Rats Group Unadjusted Mortality (Rate) Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 39/70 .557 .778 .059 2 Low 40/60 .667 .800 .057 3 Mid 33/60 .550 .660 .067 4 Mid-High 25/60 .417 5 High 29/70 .414 .500 .565 .071 .070 Covance 6329-183 3M T-6295 6 High Recovery 30/40 .750 .750 .068 Trend (Groups 1 - 5) p (one-sided) Groups 1 vs 2 p (two-sided) Groups 1 vs 3 p (two-sided) Groups 1 vs 4 p (two-sided) Groups 1 vs 5 p (two-sided) Groups 1 vs 6 p (two-sided) Groups 5 vs 6 p (two-sided) Cox-Tarone Test .0005 -** .9849 .1820 .0115 -* .0292 -* .7372 .0980 + 64 Text Table 2 Results of Statistical Analyses of Survival Data for Female Rats Group Unadjusted Mortality (Rate) Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 26/70 .371 .520 .071 2 Low 35/60 .583 .700 .065 3 Mid 41/60 .683 .820 .054 4 Mid-High 35/60 .583 5 High 25/70 .357 .700 .498 .065 .071 Covance 6329-183 3M T-6295 6 High Recovery 23/40 .575 .575 .078 Trend (Groups 1 - 5) p (one-sided) Groups 1 vs 2 p (two-sided) Groups 1 vs 3 p (two-sided) Groups 1 vs 4 p (two-sided) Groups 1 vs 5 p (two-sided) Groups 1 vs 6 p (two-sided) Groups 5 vs 6 p (two-sided) Cox-Tarone Test .3394 .1700 + .0015 +** .2338 + .8644 .9407 + .6113 + 65 Covance 6329-183 3M T-6295 Text Table 3 Results of Statistical Analyses of Nonneoplastic Lesions in the Males 1 2 Group Control Low Liver - Infiltrate, Lymphohistiocytic 51/65 43/55 p-values .1836 - .5720 - 3 Mid 44/55 .5090 + 4 Mid-High 45/55 .4111 + 5 High 45/65 .1592 - 6 High Recovery Groups 5 vs. 6 31/40 NA .2450 + Liver - Hypertrophy, Hepatocellular Centrilobular 0/65 2/55 4/55 .0000 +** .2080 + .0415 +* 22/55 .0000 +** 42/65 .0000 +** 3/40 .0527 + .0000 -** Liver - Granular Cytoplasm, Eosinophilic, Centrilobular 0/65 0/55 0/55 0/55 .0000 +** NA NA NA 14/65 0/40 .0000 +** NA .0007 -** Liver - Pigment Hepatocellular, Centrilobular 0/65 0/55 0/55 0/55 6/65 0/40 .0006 +** NA NA NA .0139 +* NA .0513 - Liver - Necrosis, Individual Hepatocyte 5/65 4/55 6/55 5/55 14/65 4/40 .0106 +* NA NA NA .0224 +* .4699 + .1024 - Liver - Vacuolation, Hepatocellular Midzonal / Centrilobular 3/65 3/55 6/55 13/55 .0000 +** NA .1696 + .0024 +** 19/65 .0001 +** 3/40 .4152 + .0060 -** 66 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 1 2 3 4 Group Control Low Mid Mid-High Liver - Focus, Altered Hepatocellular, Clear / Eosinophilic Cell 20/65 21/55 23/55 24/55 p-values .1948 + NA .1431 + .1026 + 5 High 24/65 .2892 + 6 High Recovery Groups 5 vs. 6 16/40 .2243 + .4553 + Liver - Focus, Altered Hepatocellular, Basophilic 10/65 7/55 16/55 17/55 13/65 8/40 p-values .0663 + .4414 - .0556 + .0352 +* .3232 + .3616 + NA Liver - Degeneration, Cystic 5/65 15/55 p-values .0007 +** .0041 +** 19/55 .0003 +** 17/55 .0011 +** 22/65 .0002 +** 15/40 .0002 +** .4306 + Liver - Necrosis, Coagulative 1/65 4/55 1/55 p-values .2563 + .1346 + NA 6/55 2/65 .0351 +* NA 4/40 .0683 + .1471 + Liver - Hematopoiesis, Extramedullary 4/65 3/55 8/55 3/55 7/65 5/40 p-values .2161 + NA .1111 + NA .2652 + .2187 + NA Liver - Hyperplasia, Bile Duct 20/65 20/55 p-values .1351 + NA 25/55 .0713 + 24/55 .1026 + 25/65 .2305 + 21/40 .0224 +* .1141 + 67 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 1 2 Group Control Low Liver - Fibrosis, Peribiliary 8/65 7/55 p-values .1057 + NA 3 Mid 11/55 .1842 + 4 Mid-High 7/55 NA 5 High 14/65 .1209 + 6 High Recovery Groups 5 vs. 6 4/40 .4901 - .1024 - Liver - Hypertrophy, Hepatocellular Periportal 4/65 1/55 7/55 p-values .3303 - .2387 - .1773 + 2/55 .4222 - 2/65 .3400 - 5/40 .2187 + .0719 + Liver - Infiltrate, Macrophage, Pigmented 6/65 5/55 3/55 4/55 5/65 p-values .3554 - NA .3357 - .4813 - NA 2/40 .3491 - .4588 - Liver - Vacuolation, Hepatocellular 6/65 2/55 3/55 1/55 7/65 3/40 p-values .4677 + .1976 - .3357 - .0881 - NA NA .4254 - Liver - Degeneration / Necrosis Centrilobular 1/65 0/55 0/55 1/55 5/65 1/40 p-values .0132 +* NA NA NA .1039 + NA .2566 - Liver - Glycogen, Hepatocellular, Increased 1/65 3/55 0/55 0/55 1/65 0/40 p-values .2206 - .2491 + NA NA NA NA NA 68 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs. 6 Liver - Angiectasis 1/65 1/55 2/55 0/55 2/65 0/40 p-values NA NA NA NA NA NA .3810 - Liver - Lipidosis Subcapsular 0/65 0/55 3/55 1/55 0/65 0/40 p-values .4317 + NA .0934 + NA NA NA NA Pancreas - Zymogen Granules, Decreased 11/65 15/54 11/55 p-values .2162 + .1145 + NA 14/55 .1785 + 16/65 .1937 + 8/40 .4406 + .3831 - Pancreas - Infiltrate, Lymphohistiocytic 2/65 2/54 1/55 4/55 1/65 1/40 p-values .4593 - NA NA .2641 + NA NA NA Pancreas - Infiltrate, Macrophage, Pigmented 24/65 17/54 18/55 p-values .1172 - .3350 - .3873 - 13/55 .0845 - 19/65 .2281 - 13/40 .4031 - .4437 + Pancreas - Atrophy, Acinar 7/65 7/54 8/55 9/55 7/65 3/40 p-values .4354 + NA NA .2642 + NA .4254 - .4254 - 69 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 1 2 3 Group Control Low Mid Pancreas - Hypertrophy / Basophilia, Acinar Cells 2/65 3/54 0/55 p-values .4568 - NA .2913 - 4 Mid-High 1/55 NA 5 High 3/65 NA 6 High Recovery Groups 5 vs. 6 3/40 .2812 + .4152 + Pancreas - Inflammation, Vascular 2/65 1/54 4/55 4/55 1/65 p-values .4632 + NA .2641 + .2641 + NA 4/40 .1471 + .0683 + Pancreas - Infiltration, Fat, Interstitial 11/65 16/54 20/55 14/55 12/65 6/40 p-values .4908 - .0768 + .0133 +* .1785 + NA NA .4302 - Pancreas - Thrombus 1/65 0/54 0/55 0/55 0/65 2/40 p-values NA NA NA NA NA .5112 - .1429 + Thyroid - Hyperplasia, C-Cell 9/60 8/49 p-values .4920 - NA 10/50 NA 7/49 9/59 .5687 - NA 4/39 .3586 - .3467 - Thyroid - Dilatation / Cyst, Follicular 6/60 3/49 p-values .0966 + .3558 - 3/50 .3440 - 9/49 .1631 + 8/59 .3756 + 2/39 .3196 - .1568 - 70 Covance 6329-183 3M T-6295 Text Table 4 Results of Statistical Analyses of Nonneoplastic Lesions in the Females 1 2 Group Control Low Liver - Infiltrate, Lymphohistiocytic 42/65 42/55 p-values .0080 +** NA 3 Mid 38/55 .3738 + 4 Mid-High 41/55 NA 5 High 56/65 .0038 +** 6 High Recovery Groups 5 vs. 6 32/40 .0709 + .2852 - Liver - Hypertrophy, Hepatocellular, Centrilobular 2/65 1/55 4/55 .0000 +** NA .2641 + 16/55 52/65 2/40 .0001 +** .0000 +** NA .0000 -** Liver - Granular Cytoplasm, Eosinophilic, Centrilobular 0/65 0/55 0/55 7/55 .0000 +** NA NA .0034 +** 36/65 .0000 +** 1/40 .3810 + .0000 -** Liver - Pigment, Hepatocellular, Centrilobular 0/65 0/55 0/55 1/55 36/65 0/40 .0000 +** NA NA NA .0000 +** NA .0000 -** Liver - Necrosis, Individual Hepatocyte 7/65 6/55 6/55 6/55 15/65 3/40 .0359 +* NA NA NA .0500 +* .4254 - .0329 -* Liver - Vacuolation, Hepatocellular, Midzonal / Centrilobular 1/65 1/55 0/55 1/55 .0753 + NA NA NA 4/65 1/40 .1826 + NA .3660 - 71 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 1 2 3 Group Control Low Mid Liver - Vacuolation, Hepatocellular, Periportal 15/65 15/55 22/55 p-values .1052 + NA .0358 +* 4 Mid-High 16/55 NA 5 High 22/65 .1217 + 6 High Recovery Groups 5 vs. 6 11/40 .3877 + .3235 - Liver - Focus, Altered Hepatocellular, Clear / Eosinophilic Cell 27/65 21/55 18/55 17/55 21/65 13/40 p-values .0985 - .4263 - .2109 - .1553 - .1818 - .2367 - NA Liver - Focus, Altered Hepatocellular, Basophilic 16/65 20/55 13/55 p-values .2314 + .1153 + .5367 - 21/55 .0801 + 20/65 .2785 + 11/40 .4573 + .4487 - Liver - Degeneration, Cystic 0/65 1/55 1/55 2/55 4/65 1/40 p-values .0187 +* NA NA .2080 + .0596 + .3810 + .3660 - Liver - Necrosis, Coagulative 3/65 2/55 4/55 2/55 2/65 2/40 p-values .3857 - NA NA NA NA NA NA Liver - Hematopoiesis, Extramedullary 10/65 17/55 15/55 8/55 7/65 5/40 p-values .0721 - .0352 +* .0851 + .5528 - .3020 - .4579 - NA 72 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 1 2 Group Control Low Liver - Hyperplasia, Bile Duct 23/65 25/55 p-values .3266 + .1749 + 3 Mid 19/55 .5389 - 4 Mid-High 17/55 .3738 - 5 High 30/65 .1421 + 6 High Recovery Groups 5 vs. 6 17/40 .2999 + .4358 - Liver - Fibrosis, Peribiliary 9/65 13/55 4/55 9/55 p-values .4341 - .1264 + .1960 - NA 10/65 NA 4/40 .3987 - .3168 - Liver - Hypertrophy, Hepatocellular, Periportal 12/65 10/55 9/55 p-values .0026 -** .5796 - .4778 - 4/55 .0614 - 3/65 7/40 .0127 -* NA .0344 +* Liver - Infiltrate, Macrophage, Pigmented 2/65 3/55 5/55 p-values .0000 +** NA .1567 + 6/55 .0889 + 23/65 .0000 +** 7/40 .0147 +* .0383 -* Liver - Vacuolation, Hepatocellular 3/65 2/55 0/55 3/55 3/65 2/40 p-values .4582 + NA .1555 - NA NA NA NA Liver - Cyst, Biliary 3/65 1/55 1/55 1/55 4/65 2/40 p-values .3708 + .3749 - .3749 - .3749 - NA NA NA 73 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 1 2 Group Control Low Liver - Inflammation, Subacute 2/65 0/55 p-values .2760 - .2913 - 3 Mid 0/55 .2913 - 4 Mid-High 0/55 .2913 - 5 High 1/65 NA 6 High Recovery Groups 5 vs. 6 0/40 .3810 - NA Liver - Angiectasis 6/65 p-values .0231 -* 1/55 .0881 - 2/55 .1976 - 1/55 .0881 - 1/65 .0574 - 2/40 .3491 - .3232 + Liver - Congestion 2/65 2/55 1/55 0/55 0/65 0/40 p-values .0451 -* NA NA .2913 - .2481 - .3810 - NA Pancreas - Zymogen Granules, Decreased 3/65 11/55 14/55 p-values .0488 +* .0093 +** .0011 +** 9/55 .0329 +* 12/65 .0127 +* 10/40 .0030 +** .2879 + Pancreas - Infiltrate, Lymphohistiocytic 3/65 0/55 2/55 3/55 3/65 1/40 p-values .2999 + .1555 - NA NA NA .5071 - .5071 - Pancreas - Hyperplasia, Islet Cells 2/65 1/55 0/55 0/55 0/65 0/40 p-values .0372 -* NA .2913 - .2913 - .2481 - .3810 - NA 74 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 1 2 Group Control Low Pancreas - Atrophy, Acinar 2/65 3/55 p-values .3303 + NA 3 Mid 5/55 .1567 + 4 Mid-High 2/55 NA 5 High 4/65 .3400 + 6 High Recovery Groups 5 vs. 6 3/40 .2812 + NA Pancreas - Hypertrophy / Basophilia, Acinar Cells 1/65 5/55 2/55 1/55 p-values .1017 - .0698 + NA NA 0/65 1/40 NA NA .3810 + Pancreas - Infiltration, Fat, Interstitial 10/65 7/55 4/55 9/55 p-values .2520 - .4414 - .1367 - NA 6/65 .2120 - 7/40 .4888 + .1721 + Thyroid - Hyperplasia, C-Cell 20/60 19/50 p-values .3153 - NA 19/50 NA 15/50 .4342 - 19/60 NA 16/40 .3190 + .2598 + 75 Covance 6329-183 3M T-6295 Text Table 5 Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 4 5 Group Control Low Mid Mid-High High Brain - Astrocytoma, Malignant Fatal Incidence 1 0 Incidental Incidence 1 0 Total Incidence Rate 2/60 0/40 0/40 1/30 0/60 6 High Recovery Groups 5 vs 6 0/34 One-sided p-value .3955 - Liver - Hepatocellular, Adenoma Fatal Incidence 0 0 1 0 0 0 Incidental Incidence 0 3 2 1 7 0 Total Incidence Rate 0/60 3/50 3/50 1/50 7/60 0/40 One-sided p-value .0276 +* .1345 + .0689 + NA .0456 +*E NA .0240 -* Pancreas - Islet Cell, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 4 3 4 4 4 1 Total Incidence Rate 4/60 3/49 4/50 4/50 4/60 1/40 One-sided p-value NA NA NA NA NA .2824 - .3021 - 76 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Pancreas - Islet Cell, Carcinoma Fatal Incidence 0 1 0 0 0 0 Incidental Incidence 1 1 2 5 5 3 Total Incidence Rate 1/60 2/49 2/50 5/50 5/60 3/40 One-sided p-value .0681 + NA NA .2487 + .2435 + .2834 + .4972 - Pancreas - Islet Cell, Adenoma/Carcinoma Fatal Incidence 0 1 0 0 Incidental Incidence 5 4 6 8 Total Incidence Rate 5/60 5/49 6/50 8/50 0 0 9 4 9/60 4/40 One-sided p-value .1341 + NA NA .2904 + .2027 + NA .3709 - Adrenal Cortex - Adenoma Fatal Incidence 0 Incidental Incidence 3 Total Incidence Rate 3/60 0/42 1/33 2/26 0 1 1/60 0/30 One-sided p-value .2238 - 77 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 Group Control Low Mid Adrenal Medulla - Pheochromocytoma, Benign Fatal Incidence 0 Incidental Incidence 3 Total Incidence Rate 3/60 6/42 5/33 4 5 Mid-High High 0 5 3/26 5/60 6 High Recovery Groups 5 vs 6 1/30 One-sided p-value .3519 + Adrenal Medulla - Pheochromocytoma, Malignant Fatal Incidence 0 Incidental Incidence 1 Total Incidence Rate 1/60 2/42 0/33 0/26 Note: Incidences across groups do not meet selection criterion. 0 1 1/60 0/30 Adrenal Medulla - Pheochromocytoma, Benign/Malignant Fatal Incidence 0 Incidental Incidence 3 Total Incidence Rate 3/60 0 6 6/60 1/30 One-sided p-value .4033 + 78 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 4 Group Control Low Mid Mid-High Testis - Interstitial Cell Tumor, Benign Fatal Incidence 0 0 0 0 Incidental Incidence 1 1 2 2 Total Incidence Rate 1/60 1/50 2/50 2/50 Note: Incidences across groups do not meet selection criterion. 5 High 0 1 1/60 6 High Recovery Groups 5 vs 6 0 1 1/39 Testis - Interstitial Cell Tumor, Malignant Fatal Incidence 0 0 0 0 Incidental Incidence 0 0 0 1 Total Incidence Rate 0/60 0/50 0/50 1/50 Note: Incidences across groups do not meet selection criterion. 0 0 0 0 0/60 0/39 Testis - Interstitial Cell Tumor, Benign/Malignant Fatal Incidence 0 0 0 0 Incidental Incidence 1 1 2 3 Total Incidence Rate 1/60 1/50 2/50 3/50 0 0 1 1 1/60 1/39 One-sided p-value .4583 + NA NA .3760 + NA NA NA 79 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - C-Cell, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 5 6 6 9 7 4 Total Incidence Rate 5/60 6/49 6/50 9/49 7/59 4/39 One-sided p-value .3844 + NA NA .2803 + .3745 + NA .3752 - Thyroid - C-Cell, Carcinoma Fatal Incidence 0 Incidental Incidence 1 Total Incidence Rate 0/60 0/49 1/50 0/49 Note: Incidences across groups do not meet selection criterion. 0 0 0 1 0/59 1/39 Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 5 6 7 9 Total Incidence Rate 5/60 6/49 7/50 9/49 0 0 7 5 7/59 5/39 One-sided p-value .4037 + NA .3945 + .2803 + .3745 + .4858 + .4952 + 80 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 3 5 4 4 4 9 Total Incidence Rate 3/60 5/49 4/50 4/49 4/59 9/39 One-sided p-value .4998 - .3967 + NA NA NA .0280 +* .0121 +* Thyroid - Follicular Cell, Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 3 1 1 2 Total Incidence Rate 3/60 1/49 1/50 2/49 0 0 1 1 1/59 1/39 One-sided p-value .2210 - .3855 - .2448 - NA .1734 - .4280 - NA Thyroid - Follicular Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 6 6 5 5 Total Incidence Rate 6/60 6/49 5/50 5/49 0 0 5 10 5/59 10/39 One-sided p-value .2520 - NA NA NA NA .0970 + .0125 +* 81 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 Group Control Low Mid Muscle Skeletal - Sarcoma Undifferentiated, Malignant Fatal Incidence 1 Incidental Incidence 1 Total Incidence Rate 2/60 0/40 0/33 4 5 Mid-High High 0 0 0/25 0/60 6 High Recovery Groups 5 vs 6 0/30 One-sided p-value .2136 - Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 18 19 37/60 21 8 29/45 20 22 42/46 14 17 31/36 12 25 37/59 15 13 28/38 One-sided p-value NA NA NA NA NA .4448 + .1455 + Skin - Keratoacanthoma Total Incidence Rate 1/60 4/43 4/37 6/31 5/60 6/33 One-sided p-value NA NA NA NA .1612 + NA NA Multiple Tumor - Lipoma Total Incidence Rate 0/60 1/43 0/50 0/50 3/60 0/40 One-sided p-value NA NA NA NA .2088 + NA .2149 - 82 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 2 3 4 Group Control Low Mid Mid-High Multiple Tumor - Liposarcoma Total Incidence Rate 0/60 0/43 0/50 0/50 Note: Incidences across groups do not meet selection criterion. 5 High 1/2 6 High Recovery Groups 5 vs 6 1/40 Multiple Tumor - Lipoma/Liposarcoma Total Incidence Rate 0/60 1/43 0/50 0/50 4/60 1/40 One-sided p-value NA NA NA NA .1143 + NA .3076 - 83 Covance 6329-183 3M T-6295 Text Table 6 Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Liver - Hepatocellular, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 0 1 1 1 5 2 Total Incidence Rate 0/60 1/50 1/49 1/50 5/60 2/40 One-sided p-value .0153 +* NA NA NA .0386 +* .2040 + .3050 - Liver - Hepatocellular, Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 0 0 0 0 Total Incidence Rate 0/60 0/50 0/49 0/50 Note: Incidences across groups do not meet selection criterion. 0 0 1 0 1/60 0/40 Liver - Hepatocellular, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 0 1 1 1 Total Incidence Rate 0/60 1/50 1/49 1/50 0 0 6 2 6/60 2/40 One-sided p-value .0057 +** NA NA NA .0204 +* .2040 + .2114 - 84 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Pancreas - Islet Cell, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 2 2 1 0 2 0 Total Incidence Rate 2/60 2/50 1/49 0/50 2/60 0/40 One-sided p-value .3126 - NA NA .3484 -E NA .2387 - .2502 - Pancreas - Islet Cell, Carcinoma Fatal Incidence 1 0 0 0 Incidental Incidence 0 0 1 0 Total Incidence Rate 1/60 0/50 1/49 0/50 Note: Incidences across groups do not meet selection criterion. 1 0 0 0 1/60 0/40 Pancreas - Islet Cell, Adenoma/Carcinoma Fatal Incidence 1 0 0 0 Incidental Incidence 2 2 2 0 Total Incidence Rate 3/60 2/50 2/49 0/50 1 0 2 0 3/60 0/40 One-sided p-value .3421 - NA NA .0513 - NA .1539 - .1750 - 85 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 0 0 0 2 1 1 Total Incidence Rate 0/60 0/50 0/49 2/50 1/60 1/40 One-sided p-value .1002 + NA NA .1054 + NA .2387 - NA Thyroid - Follicular Cell, Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 0 0 0 1 Total Incidence Rate 0/60 0/50 0/49 1/50 Note: Incidences across groups do not meet selection criterion. 0 0 0 0 0/60 0/40 Thyroid - Follicular Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 0 0 0 3 Total Incidence Rate 0/60 0/50 0/49 3/50 0 0 1 1 1/60 1/40 One-sided p-value .0790 + NA NA .0471 +* NA .1539 - NA 86 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - C-Cell, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 12 6 6 8 5 6 Total Incidence Rate 12/60 6/50 6/49 8/50 5/60 6/40 One-sided p-value .0703 - .1478 - .1705 - .2103 - .0336 -* .2392 - .3548 + Thyroid - C-Cell, Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 0 1 0 0 Total Incidence Rate 0/60 1/50 0/49 0/50 Note: Incidences across groups do not meet selection criterion. 0 0 0 1 0/60 1/40 Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 12 7 6 8 Total Incidence Rate 12/60 7/50 6/49 8/50 0 0 5 7 5/60 7/40 One-sided p-value .0560 - .2284 - .1705 - .2103 - .0336 -* .3380 - .2399 + 87 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats Group Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 1 Control 18 28 46/60 2 Low 23 18 41/50 3 Mid 32 10 42/46 4 5 Mid-High High 23 14 37/46 15 25 40/60 6 High Recovery Groups 5 vs 6 16 14 30/34 One-sided p-value NA .2409 + NA NA .1726 - NA NA Skin - Squamous Cell, Papilloma Total Incidence Rate 2/60 0/36 0/40 0/35 0/60 1/25 One-sided p-value NA NA NA NA .2209 - NA NA Skin - Squamous Cell, Carcinoma Total Incidence Rate 1/60 0/36 0/40 0/35 Note: Incidences across groups do not meet selection criterion. 0/60 0/25 Skin - Squamous Cell, Papilloma/Carcinoma Total Incidence Rate 3/60 0/36 0/40 0/35 0/60 1/25 One-sided p-value NA NA NA NA .1140 - NA NA 88 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Mammary Female- Fibroadenoma Total Incidence Rate 20/60 2 Low 27/50 3 Mid 19/48 4 5 Mid-High High 24/50 11/60 6 High Recovery Groups 5 vs 6 15/40 One-sided p-value .0152 -* .0337 +* .1125 + .2521 + .0235 -* .4099 - .0573 + Mammary Female- Adenoma Total Incidence Rate 7/60 6/50 5/48 7/50 4/60 4/40 One-sided p-value .2359 - NA .4086 + NA .2315 - NA .5055 + Mammary Female- Fibroadenoma/Adenoma Total Incidence Rate 23/60 30/50 22/48 26/50 15/60 16/40 One-sided p-value .0239 -* .0318+* .0527 + .2753 + .0488 -* NA .1451 + Mammary Female- Carcinoma Total Incidence Rate 11/60 12/50 15/48 11/50 14/60 4/40 One-sided p-value .3569 .3996 + .0515 + NA .3373 + .0606 - .0161 -* 89 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 2 3 Group Control Low Mid Mammary Female- Fibroadenoma/Adenoma/Carcinoma Total Incidence Rate 29/60 36/50 31/48 4 5 Mid-High High 29/50 24/60 6 High Recovery Groups 5 vs 6 17/40 One-sided p-value .0482 -* .0474 +* .0066 +** NA .1479 - .0598 - .3482 - 90 Covance 6329-183 3M T-6295 TEXT FIGURE 1 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: MALE RATS 0 15 30 45 60 75 90 105 + + + + + + + + ------------------------------------------------------------------------- 1.0+************************************** + 11***************** 3 *555555 + 11111**********22*22222 + *******05 22 + 4****** 222 .9+ ***1*** 22 P+ 0*******33** R+ 0*04* * O+ 5*0***1* P+ 500 41* O .8+ 5500***33 R+ 5550 *23 T+ 500**** I+ 550**4**3 O+ 55**12*** N .7+ 05*122**444 + 0 51 2 3 4 S+ 0 51 2 3 4 U+ 0 5112233 444 R+ 00*5* 223 4 V .6+ 00***2* 44 I+ 0 *5*2 4 V+ 0 1 *22 44 I+ 00 1 *3*33 4 N+ 00*155* 33*33 G .5+ 011 * 44*3 + 00* * 4 + * *22 44 + ***** 4 + 0** .4+ **2 + ***22 + **122 + 0** 22 + 0*55 .3+ *5 + ***5 + *5 + *55 + ** .2+ 1 + + 0 = Group 1 (Control) + 1 = Group 2 (Low) + 2 = Group 3 (Mid) .1+ 3 = Group 4 (Mid-High) + 4 = Group 5 (High) + 5 = Group 6 (High Recovery) + * = More Than One Data Point + .0+ ------------------------------------------------------------------------- + + + + + + + + 0 15 30 45 60 75 90 105 TIME (WEEKS) 91 Covance 6329-183 3M T-6295 TEXT FIGURE 2 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: FEMALE RATS 0 15 30 45 60 75 90 105 + + + + + + + + ------------------------------------------------------------------------- 1.0+****************************00000000000 + 222222222222222********************555 + 111**11 33*******3 + 22**************** + *** 5*55 .9+ 1*4 ***5 P+ ***44*****55 R+ 2 11 4* 0 5 O+ 222* *40 55 P+ *1 3**0 5 O .8+ 2* 3**4** R+ ***11*** **4 T+ 22 ** 5444 I+ 222 0**** 4 O+ 2 01*54 N .7+ 22 0***4 + 2 0*** S+ 22 *3** U+ 2 *15*4 R+ 2 0****4 V .6+ 2 *034444 I+ 2 ***5 4 V+ 22 0** 4 I+ 2 1*5 44 N+ 22 1******4 G .5+ 2 ** 00** + 2 * 5* + 2222 *3 5 + 22 13 5 + 2 1* 55 .4+ 2 *3 + 2 1* + 2 **3 + 2 133 + 222 113 .3+ 22 1* + 22 + 2 + 222 + 22 .2+ 22 + 2 + + 0 = Group 1 (Control) + 1 = Group 2 (Low) .1+ 2 = Group 3 (Mid) + 3 = Group 4 (Mid-High) + 4 = Group 5 (High) + 5 = Group 6 (High Recovery) + * = More Than One Data Point .0+ ------------------------------------------------------------------------- + + + + + + + + 0 15 30 45 60 75 90 105 TIME (WEEKS) 92 Covance 6329-183 3M T-6295 COMMENTS ON THE DATA Various models of calculators, computers, and computer programs were used to analyze data in this study. Because different models round off or truncate numbers differently, values in some tables (e.g., means, standard deviations, or individual values) may differ slightly from those in other tables, from individually calculated data, or from statistical analysis data. Neither the integrity nor the interpretation of the data was affected by these differences. The units for the dose levels in the inlife summary tables are ppm. The number of animals listed in the heading of the summary tables for clinical and mass observations reflects the number of animals assigned to each group at the start of the study. The summary tables for clinical observations indicate the number of animals for which a condition was observed without regard to the reversibility, number of incidences/animal, or the length of time the condition persisted. Only observations for each animal other than normal are indicated in the summary clinical observations tables. Each animal with observations recorded as "Normal" throughout the study has the comment "No remarkable observations" indicated in the individual clinical observations tables. The specific details for comments in the individual clinical observations tables that are indicated with a "C" can be found at the end of each group for each sex. The level of severity for some clinical observations was not specified at the time of the observation and is indicated as <UNSPECIFIED> in the summary and individual clinical observation tables. The day of initiation of treatment is "Day 1, Week 1." Body weight data are entered at the start of a study week (e.g., a body weight recorded on Day 1 is considered a Week 1 body weight, a body weight recorded on Day 8 is considered a Week 2 body weight). Body weight change data are calculated from the first day of the study week to the first day of the following study week (e.g., Week 1 values are calculated from Day 1 through 8). Weekly food consumption are calculated from the first day of the study week to the first day of the following study week (e.g., Week 1 values are calculated from Day 1 through 8). 93 Covance 6329-183 3M T-6295 COMMENTS ON THE DATA (Continued) The values for summary and individual test material consumption are reported with more significant figures than is appropriate for the data due to limitations of the data collection and reporting software. The comment "SPILLED" in individual food consumption data tables indicates that food consumption was not recorded due to spillage during the interval. The comment "CNTMD" in individual food consumption data tables indicates that food consumption was not recorded due to contamination of the diet with urine or feces in the food container. The comment "NOT TAKEN" in individual food consumption data tables indicates that the animal died before the end of the food consumption interval. The comment "TECH SPILL" in individual food (water) consumption data tables indicates that food consumption was not recorded due to food (water) spillage caused by technician error. Differences in the population size (N) in the summary tables for clinical and anatomic pathology are explained in the individual data tables or the code sheets. Results of clinical pathology samples collected for animals sacrificed at unscheduled intervals appear in the individual clinical pathology tables for the next scheduled interval. These results are not in the summary tables and are not included in the statistical analyses. Statistical analyses were not done when there were two or fewer values for a parameter. 94 Covance 6329-183 3M T-6295 COMMENTS ON THE DATA (Continued) The calculation for individual test material consumption is: Test material consumption = Diet ConcentrationxFood Consumption Food Consumption Intervalx[First Body Weight of Interval+ (Daily Body Weight GainxDay Factor)] where: Daily Body Weight Gain = Last Body Weight of Interval- First Body Weight of Interval Day of Last Body Weight of Interval- Day of First Body Weight of Interval and: Day Factor = (Food Consumption Start Day- Day of First Body Weight of Interval) + Food Consumption Interval 2 and: Food Consumption Interval = Food Consumption End Day- Food Consumption Start Day 95 CODES, ABBREVIATIONS, AND UNITS Covance 6329-183 3M T-6295 General Codes and Abbreviations Codes for Clinical Pathology Abbreviations and Units for Clinical Hematology Abbreviations and Units for Clinical Chemistry Abbreviations and Units for Clinical Urinalysis Abbreviations and Units for Urine Chemistry Codes for Anatomic Pathology Note: The following lists of codes, abbreviations, and units are used by Covance. Some, but not necessarily all, of this information may be needed for this report. 96 Covance 6329-183 3M T-6295 General Codes and Abbreviations WK N Mean; MEAN CAM SD; S.D.; STAND DEV; STANDARD DEV; sd * -; NA P C UNSCHED DISPATCH TBW # CO Week Number of measurements in a group Arithmetic mean Covariate-adjusted mean Standard deviation Group mean is significantly different from the mean of the control group (Group 1) at p < 0.05 No value; not applicable; not present Present Comment found at the end of each group for each sex Unscheduled Observations transferred from the inlife module of the data collection system to the necropsy module for reference during necropsy. Observations are duplicates of the last inlife observations. Terminal body weight Number Clinical observation Animal Death Codes 1 2 3 T D M U Interim sacrifice, Week 4 Interim sacrifice, Week 14 Interim sacrifice, Week 53 Terminal sacrifice Found dead Moribund First postrecovery sacrifice 97 NS QS/QNS NR FS SC SH H SL L SI I NF U DT/DOT DB TJ TE RE EE SE PC PD PI PL PA CO HB PLASMO NO AGG FR UTD NO COAG Covance 6329-183 3M T-6295 Codes for Clinical Pathology GENERAL CODES No sample Quantity not sufficient No repeat (sample volume not sufficient for repeat analysis) Fibrin strands Sample clotted Slightly hemolyzed Hemolyzed Slightly lipemic Lipemic Slightly icteric Icteric Animal not fasted Unscheduled/moribund bleed Animal died on test Died during bleeding Technician judgment to repeat test Technical error (instrument or technician error that results in unacceptable data, e.g., unacceptable instrument output, sample spilled, entry of invalid data) Recording error (recorded incorrect data, e.g., wrong number, spelling error, incorrect date) Entry error (incorrect keyboard entry) Sampling error Platelets clumped Platelets decreased Platelets increased Platelets large Platelets appear adequate Color interferes with test Heinz bodies observed Plasmodium No aggregation Fractious Unable to determine No coagulation 98 Covance 6329-183 3M T-6295 Codes for Clinical Pathology (Continued) RESULTS NOT INCLUDED IN STATISTICAL ANALYSES Hemolyzed clinical chemistry or coagulation samples Samples from animals at unscheduled intervals Prothrombin times (PT) greater than 50 seconds Activated partial thromboplastin times (PTT) greater than 110 seconds Bleed times (BLETIME) greater than 30 minutes CODES FOR BLOOD CELL MORPHOLOGY The following scale was used to measure the degree of anisocytosis (ANISO), poikilocytosis (POIK), polychromasia (POLY), hypochromasia (HYPO), or basophilic stippling (BASTIP) or the presence of Howell-Jolly bodies (HJBODY), toxic neutrophils (TOXNEUT), or atypical lymphocytes (ATYPLYM). Scale 1 2 3 4 Degree Normal for the species Slight Moderate Marked Not applicable Presence Not present Rare Few Moderate Many URINE APPEARANCE A Pale B Straw C Yellow D Dark yellow Color E Amber F Brown G Red H Green I Black P Blue/green Q Blue R Orange Clarity J Clear K Hazy L Cloudy Miscellaneous M Debris O Feces 99 Covance 6329-183 3M T-6295 Codes for Clinical Pathology (Continued) URINE CHEMISTRY MULTISTIX STRIP Urine Glucose + ++ +++ ++++ +++++ Negative 100 mg/dL 250 mg/dL 500 mg/dL 1,000 mg/dL >2,000 mg/dL Urine Ketone + ++ +++ ++++ +++++ Negative 5 mg/dL 15 mg/dL 40 mg/dL 80 mg/dL 160 mg/dL Urine Blood - Negative + Small ++ Moderate +++ Large Urine Urobilinogen + ++ +++ ++++ 0.2 mg/dL 1 mg/dL 2 mg/dL 4 mg/dL 8 mg/dL (1 mg = approximately 1 Ehrlich unit) Urine Bilirubin - Negative + Small ++ Moderate +++ Large URINE SEDIMENT Cells, Crystals, Casts, and Comments A Amorphous urates B Amorphous phosphates C Uric acid D Triple phosphates E Calcium oxalate F Calcium carbonate G Granular casts H Hyaline casts I Cellular casts J Waxy casts K Unknown crystal P Mucous threads Q Sperm R Fecal contamination S Pinworm ova found T Pinworm larvae found U Parasite ova found 0 Not present 1 1 - 5 per field 2 6 - 10 per field 3 11 - 20 per field 4 >20 per field Bacteria 0 Not present 1 Few 2 Moderate 3 Many 100 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Hematology Test Red blood cell count Hemoglobin Hematocrit Mean corpuscular volume Mean corpuscular hemoglobin Mean corpuscular hemoglobin concentration Platelet count Mean platelet volume Reticulocyte count Absolute reticulocyte count Heinz body count Erythrocyte sedimentation rate Prothrombin time Activated partial thromboplastin time Thrombin time Activated coagulation time Fibrinogen Fibrin/fibrinogen degradation products Platelet aggregation Collagen Adenosine diphosphate Alpha 2-antiplasmin Bleeding time Methemoglobin Plasma hemoglobin Myeloid/erythroid ratio Estimated myeloid/erythroid ratio White blood cell count Differential blood cell count Nucleated red blood cell count Corrected white blood cell count Segmented neutrophil count Band neutrophil count Lymphocyte count Monocyte count Eosinophil count Basophil count Anisocytosis Polychromasia Abbreviation (Units) RBC (E6/UL or X106/L) HGB (G/DL) HCT (%) MCV (FL) MCH (PG) MCHC (%) PLT (E3/UL or X103/L) MPV (FL) RETIC (%) RETIC (E3/UL or X103/L) HEINZ (%) ESR (MM/HR) PT (SEC) PTT (SEC) TT (SEC) ACT (SEC) FBR (MG/DL) FDP (UG/ML) PAGG/COL (%) PAGG/ADP (%) ANTIPLAS (%) BLE TIME (SEC) METHGB (%) PLA HGB (MG/DL) M/E RATIO EST M/E RATIO WBC (E3/UL or X103/L) NRBC (/100 WBC) COR WBC (E3/UL or X103/L) N-SEG (E3/UL or X103/L) and % N-BAND (E3/UL or X103/L) and % LYMPH (E3/UL or X103/L) and % MONO (E3/UL or X103/L) and % EOSIN (E3/UL or X103/L) and % BASO (E3/UL or X103/L) and % ANISO (-,1,2,3) POLY (-,1,2,3) 101 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Hematology (Continued) Test Poikilocytosis Hypochromasia Howell-Jolly bodies Basophilic stippling Toxic neutrophils Atypical lymphocytes Aqueous white blood cell count (right eye) Aqueous white blood cell count (left eye) Abbreviation (Units) POIK (-,1,2,3) HYPO (-,1,2,3) HJBODY (-,1,2,3,4) BASTIP (-,1,2,3) TOXNEUT (-,1,2,3,4) ATYPLYM (-,1,2,3,4) R EYE (WBC/UL) L EYE (WBC/UL) 102 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Chemistry Test Glucose Urea nitrogen Urea Creatinine Total protein Albumin Globulin Albumin/globulin ratio Total bilirubin Direct bilirubin Indirect bilirubin Cholesterol Triglyceride Urea nitrogen/creatinine ratio Total lipids Phospholipids High-density lipoprotein cholesterol Low-density lipoprotein cholesterol Uric acid Aspartate aminotransferase Alanine aminotransferase Alkaline phosphatase Gamma glutamyltransferase Sorbitol dehydrogenase Lactate dehydrogenase Creatine kinase Amylase Lipase Palmitoyl CoA oxidase Calcium Ionized calcium Inorganic phosphorus Sodium Potassium Chloride Magnesium Zinc Strontium Iron Abbreviation (Units) GLU (MG/DL) UN (MG/DL) UREA (MG/DL) CREAT (MG/DL) T PRO (G/DL) ALB (G/DL) GLOB (G/DL) A/G RATIO T BILI (MG/DL) D BILI (MG/DL) I BILI (MG/DL) CHOL (MG/DL) TRIG (MG/DL) UN/CREAT (RATIO) T LIPIDS (MG/DL) P LIPIDS (MG/DL) HDL (MG/DL) LDL (MG/DL) UA (MG/DL) AST/SGOT (IU/L) ALT/SGPT (IU/L) ALK PHOS (IU/L) GGT (IU/L) SDH (IU/L) LDH (IU/L) CK (IU/L) AMYLASE (IU/L) LIPASE (IU/L) PCOAO (IU/G) CA (MG/DL) ION CA (MG/DL) I PHOS (MG/DL) NA (MMOL/L) K (MMOL/L) CL (MMOL/L) MG (MEQ/L or MG/DL) ZN (MG/L or PPM) SR (MG/L or PPM) FE (UG/DL) 103 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Chemistry (Continued) Test Excess iron Total iron binding capacity Unbound iron binding capacity Percent iron saturation Plasma cholinesterase Red blood cell cholinesterase Brain cholinesterase Caudate putamen Hippocampus Frontal cortex Cerebellum Bicarbonate Serum hemoglobin Serum bile acids Fecal bile acids Average fecal weight Fecal bile acids (calculation) Osmolality Electrophoresis Albumin Alpha-1-globulin Alpha-2-globulin Beta globulin Gamma globulin High-density lipoprotein Low-density lipoprotein Very-low-density lipoprotein Insulin Adrenocorticotropic hormone Cortisol Glucagon Triiodothyronine Thyroxine Creatine kinase isoenzymes BB MB MM Abbreviation (Units) EX FE (UG/DL) TIBC (UG/DL) UIBC (UG/DL) FE %SAT (%) CHEP (MU/ML) CHER (MU/ML) CHEB (MU/ML) CAUD PUT (UMOL/G) HIPPOCAM (UMOL/G) F CORTEX (UMOL/G) CEREBELL (UMOL/G) BICARB (MMOL/L) SER HGB (MG/DL) SBA (UMOL/L or MG/DL) FBA (UG/ML) FCC WGT (G) FBA (MG/Day) OSMO (MOSM/KG) E ALB (G/DL) E A-1 (G/DL) E A-2 (G/DL) E BETA (G/DL) E GAMMA (G/DL) E-HDL (%) E-LDL (%) E-VLDL (%) INSULIN (UU/ML) ACTH (PG/ML) CORTISOL (UG/ML) GLUCAGON (PG/ML) T3 (NG/DL) T4 (UG/DL) CK-BB (U/L) CK-MB (U/L) CK-MM (U/L) 104 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Urinalysis Test Urine volume 8-hour urine volume Specific gravity Urine osmolality Quantitative urinary/cerebrospinal fluid protein Urine protein excretion Urine chemistry Multistix strip Urine pH Urine protein Urine glucose Urine ketones Urine bilirubin Urine blood Urine urobilinogen Urine reducing substances Microscopic examination of urine sediment Red blood cells per high-power field White blood cells per high-power field Epithelial cells per high-power field Bacteria per high-power field Casts per low-power field Crystals per low-power field Urine appearance Comments Abbreviation (Units) U VOL (ML) 8 HR VOL (ML) SP GR U OSMO (MOSM/KG) QUAN PRO (MG/DL) PRO EXC (MG) U PH U PRO (MG/DL) U GLU U KET U BILI U BLOOD UROBILI U RE SUB RBC (PER HPF) WBC (PER HPF) EPITH (PER HPF) BACT (PER HPF) CASTS (PER LPF) CRYSTALS (PER LPF1 or PER LPF2) URINE APP1 or URINE APP2 COMMENTS 105 Covance 6329-183 3M T-6295 Abbreviations and Units for Urine Chemistry Test Urine volume Urine osmolality Urine creatinine Urine calcium Urine calcium/creatinine ratio Urine phosphorus Urine sodium Urine potassium Urine chloride Urine magnesium Urine zinc Urine strontium Urine hydroxyproline N-ACETYL--D-glucosaminedase Creatinine excretion Calcium excretion Phosphorus excretion Sodium excretion Potassium excretion Chloride excretion Calcium fractional clearance Phosphorus fractional clearance Sodium fractional clearance Potassium fractional clearance Chloride fractional clearance Creatinine clearance Quantitative urinary/cerebrospinal fluid protein Protein to urinary creatinine ratio Urine protein excretion Abbreviation (Units) U VOL (ML) U OSMO (MOSM/KG) U CREAT (MG/DL) U CA (MG/DL) CA/CREAT (RATIO) U PHOS (MG/DL) U NA (MMOL/L) U K (MMOL/L) U CL (MMOL/L) U MG (MG/L or PPM) U ZN (MG/L or PPM) U SR (MG/L or PPM) U HYP (MG/L) NAG (U/L) CREA EXC (MG) CA EXC (MG) P EXC (MG) NA EXC (MMOL) K EXC (MMOL) CL EXC (MMOL) CA CLEAR (%) P CLEAR (%) NA CLEAR (%) K CLEAR (%) CL CLEAR (%) CREAT CL (ML/MIN) QUAN PRO (MG/DL) PRO/CREA (RATIO) PRO EXC (MG) 106 Code 1-6 T U V A B D M P R O Codes for Anatomic Pathology Definition ANIMAL DEATH CODES Interim sacrifices 1 through 6 Terminal sacrifice First postrecovery sacrifice Second postrecovery sacrifice Accidental death Death or sacrifice postaccident Found dead Moribund Returned to stock Unscheduled recovery sacrifice Other type of unscheduled death Covance 6329-183 3M T-6295 EX NOT TAKEN MISSING UNSUITABLE AUTOLYTIC EXCLUDE MACROSCOPIC CODES Indicates that organ weight is excluded from calculations Organ weight not taken; explanation given in necropsy notes Organ missing or lost Organ technically unsuitable for weighing Organ autolyzed and could not be weighed Weight was taken, but was excluded from all calculations MICROSCOPIC CODES Codes Prefacing Neoplastic Findings B- Primary, benign neoplasm M- Primary, malignant neoplasm N- Metastatic neoplasm I- Locally invasive neoplasm X- Other neoplasm Distribution of Findings Focal Diffuse Multifocal 107 Covance 6329-183 3M T-6295 Codes for Anatomic Pathology (Continued) Grades for Severity or Amount 1 Minimal - the least amount of change that can be observed with the light microscope 2 Slight - less than average amount of change, but readily discernible as abnormal 3 Moderate - the average amount of change that is expected for a lesion 4 Moderately severe (marked) - a marked amount of change with possible loss of function of the affected cells or organs 5 Severe - a great amount of change with probable loss of function of the affected cell or organs and frequently involves large areas of the organ Other Microscopic Codes TL Total P Finding present - Finding not present MN Mean TISSUE ABBREVIATIONS Abbreviation LN GL STOMACH, GL STOMACH, NONGL SALIV GL, MANDIB LN, ANT MES/PANC AUDITORY SEB GL LACRIMAL GLAND, EX HEMATO NEOPLASIA LACRIMAL GL, INT CAVITY, ABDOM SALIV GL,PAROTID LN, TRACHEOBRON CATHETER EXIT CATHETER ENTRANC CATHETER TIP Definition Lymph node Gland Glandular stomach Nonglandular stomach Mandibular salivary gland Anterior mesenteric/pancreatic lymph node Auditory sebaceous gland Exorbital lacrimal gland Hematopoietic neoplasia Internal lacrimal gland Abdominal cavity Parotid salivary gland Tracheobronchial lymph node Catheterization site: exit site from the body Catheterization site: entrance site into the vessel Catheterization site: tissues (vascular or extravascular) associated with the catheter near its tip 108 100 90 80 70 60 50 40 30 20 10 0 1 Figure 1 Survival Data - Males Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Group 1 - 0 ppm 100 Group 2 - 0.5 ppm 100 Group 3 - 2 ppm 100 Group 4 - 5 ppm 100 Group 5 - 20 ppm 100 Group 6 - 20 ppm 100 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week % 109 100 90 80 70 60 50 40 30 20 10 0 1 Figure 2 Survival Data - Females 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 Group 1 - 0 ppm 100 Group 2 - 0.5 ppm 100 Group 3 - 2 ppm 100 Group 4 - 5 ppm 100 Group 5 - 20 ppm 100 Group 6 - 20 ppm 100 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week % 110 900 800 700 600 500 400 300 200 100 0 1 Figure 3 Mean Body Weight Data (g) - Males 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 Group 1 - 0 ppm Group 2 - 0.5 ppm Group 3 - 2 ppm Group 4 - 5 ppm Group 5 - 20 ppm Group 6 - 20 ppm 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week (g) 111 Figure 4 Mean Body Weight Data (g) - Females Covance 6329-183 3MT-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS 700 600 500 400 (g) 300 Group 1 - 0 ppm Group 2 - 0.5 ppm 200 Group 3 - 2 ppm Group 4 - 5 ppm Group 5 - 20 ppm Group 6 - 20 ppm 100 0 1 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week 112 Figure 5 Mean Food Consumption Data (g) - Males Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS 215 195 175 155 135 GGrroouupp -20- p0p.5mppm Group 3 - 2 ppm Group 4 - 5 ppm Group 5 - 20 ppm 115 Group 6 - 20 ppm 95 75 1 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week (g) 113 165 155 145 135 125 115 105 95 85 75 1 Figure 6 Mean Food Consumption Data (g) - Females 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 Group - 0 ppm Group 2 - 0.5 ppm Group 3 - 2 ppm Group 4 - 5 ppm Group 5 - 20 ppm Group 6 - 20 ppm 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week (g) 114 Covance 6329-183 3M T-6295 Table 1 Results of Homogeneity Analyses (ppm) Mixed 3/18/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Samples Location Replicate 0.5 T-6295 (ppm) 20 Top Middle Bottom 1 2 Mean 1 2 Mean 1 2 Mean 0.605 0.588 0.597 (119)a 0.657 0.690 0.674 (135) 0.601 0.631 0.616 (123) 21.3 21.4 21.4 (107) 23.6 23.5 23.6 (118) 24.6 20.4 22.5 (113) a Each value in parentheses is the percent of theoretical. 115 Covance 6329-183 3M T-6295 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 4/16/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Sample Location Replicate 0.5 T-6295 (ppm) 2 5 20 Top Middle Bottom 1 2 3 Mean 1 2 3 Mean 1 2 3 Mean 0.743 0.521 0.406 0.557 (111)a 2.22 2.03 2.12 2.12 (106) 5.36 5.48 4.04 4.96 (99.2) 18.1 17.4 18.4 18.0 (90.0) 0.478 0.478 0.486 0.481 (96.2) 2.28 2.01 2.09 2.13 (107) 4.90 4.90 5.28 5.03 (101) 19.7 19.5 21.3 20.2 (101) 0.955 0.519 0.359 0.611 (122) 2.04 2.03 1.84 1.97 (98.5) 4.70 5.00 4.94 4.88 (97.6) 17.6 18.7 18.4 18.2 (91.0) a Each value in parentheses is the percent of theoretical. 116 Covance 6329-183 3M T-6295 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 04/22/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Sample Location Replicate T-6295 (ppm) 20 Top Middle Bottom 1 2 Mean 1 2 Mean 1 2 Mean 18.9 18.4 18.7 (93.5)a 20.1 19.6 19.9 (99.5) 21.1 20.1 20.6 (103) a Each value in parentheses is the percent of theoretical. 117 Covance 6329-183 3M T-6295 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 6/18/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Sample Location Replicate 0.5 T-6295 (ppm) 2 5 20 Top Topb Middle Bottom 1 2 3 Mean 1 2 3 Mean 1 2 3 Mean 1 2 3 Mean 0.566 1.10 0.955 0.874 (175) 2.38 2.71 2.23 2.44 (122) 0.483 - 0.480 - 0.401 - 0.455 (91.0) - 0.617 0.637 0.635 0.630 (126) 2.50 2.11 2.05 2.22 (111) 0.640 0.694 0.532 0.622 (124) 3.08 2.29 2.41 2.59 (130) 4.87 5.18 4.90 4.98 (99.6) 20.3 18.7 18.6 19.2 (96.0) - - - - - - - - 5.15 5.43 4.70 5.09 (102) 18.3 17.8 18.2 18.1 (90.5) 5.08 5.12 5.15 5.12 (102) 19.4 20.5 18.2 19.4 (97.0) a Each value in parentheses is the percent of theoretical. b Reassay. 118 Covance 6329-183 3M T-6295 Table 2 Results of Stability Analyses (ppm) Mixed 3/18/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Storage Conditions Replicate 0.5 T6295 (ppm) 1.0 2.0 20 Initiala 1 2 Mean 0.657 0.690 0.674 (135)b 1.15 1.51 1.33 (133) 2.29 2.67 2.48 (124) 23.6 23.5 23.6 (118) 28 days, room temperature 1 2 Mean 0.507 0.554 0.531 (106) 1.11 0.937 1.02 (102) 1.94 2.01 1.98 (99.0) 20.7 20.8 20.8 (104) a Results for the 0.5- and 20- levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in the parenthesis is the percent of theoretical. 119 Covance 6329-183 3M T-6295 Table 2 (Continued) Results of Stability Analyses (ppm) Mixed 4/16/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Storage Conditions Replicate 0.5 T6295 (ppm) 2.0 5.0 20 Initiala 1 2 3 Mean 0.478 0.478 0.486 0.481 (96.2)b 2.28 2.01 2.09 2.13 (107) 4.90 4.90 5.28 5.03 (101) 19.7 19.5 21.3 20.2 (101) 33 days, room temperature 1 2 Mean 0.527 0.553 0.540 (108) 2.57 2.54 2.56 (128) 5.64 5.83 5.74 (115) 28.4 22.1 25.6 (128) a Results were derived from the sample collected from the middle of the dose preparations for homogeneity analysis b Each value in the parenthesis is the percent of theoretical. 120 Covance 6329-183 3M T-6295 Table 3 Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0.5 T-6295 (ppm) 1 2 20 3/18/98 1 2 Mean 0.657 0.690 0.674 (135)b 1.15 1.51 1.33 (133) 2.29 23.6 2.67 23.5 2.48 (124) 23.6 (118) a Results for the 0.5- and 20- levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in the parentheses is the percent of theoretical. 121 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats T-6295 (ppm) Mix Date Replicate 0 0.5 2 5 20 4/16/98a 1 b 2 b 3 - Mean - 0.478 0.478 0.486 0.481 (96.2)c 2.28 2.01 2.09 2.13 (107) 4.90 4.90 5.28 5.03 (101) 19.7 19.5 21.3 20.2 (101) 4/23/98 4/23/98d 4/23/98e 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 0.375 0.379 0.377 (75.4) 2.07 1.76 1.92 (96.0) 6.54 4.92 5.73 (115) 19.9 21.3 20.6 (103) 0.674 - 0.535 - 0.605 (121) - 0.780 - 4.78 - 2.78 (55.6) - 0.517 - 0.406 - 0.462 (92.4) - 4.55 - 6.12 - 5.34 (107) - 4/30/98 4/30/98d 1 b 2 b Mean - 1 - 2 - Mean - 0.551 0.478 0.515 (103) - 2.12 2.11 2.12 (106) - 5.55 6.03 5.79 (116) 5.24 5.24 5.24(105) 22.6 22.1 22.4 (112) - a Results for the 0.5-, 2.0-, 5.0- and 20-ppm levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Retention. 122 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats T-6295 (ppm) Mix Date Replicate 0 0.5 2 5 20 5/7/98 5/7/98d 5/7/98e 1 2 Mean 1 2 Mean 1 2 Mean 0.962 0.942 0.952 0.406 0.390 0.398 (79.6)c 1.73 1.74 1.74 (87.0) 4.37 4.31 4.34 (86.8) 18.2 18.2 18.2 (91.0) 1.11 0.202 - - - 1.12 0.557 - - - 1.12 0.380 (76.0) - - - b 0.476 - - - b 0.347 - - - - 0.412 (82.4) - - - 5/14/98 5/21/98f 5/21/98d 1 2 Mean 1 2 Mean 1 2 Mean b 0.474 2.52 5.48 19.8 b 0.402 2.54 5.06 21.5 - 0.438 (87.6) 2.53 (127) 5.27 (105) 20.7 (104) <0.75 <0.75 - 0.157 0.349 0.253 (50.6) 1.60 1.86 1.73 (86.5) 4.98 6.27 5.63 (113) 19.6 20.9 20.3 (102) b 0.248 - - - b 0.326 - - - - 0.287 (57.4) - - - 5/28/98 1 b 0.442 1.95 6.19 2 b 0.453 2.29 6.49 Mean - 0.448 (89.6) 2.12 (106) 6.34 (127) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Retention. f The low standard (0.4 ppm) was not used for the calibration curve. 21.8 22.3 22.1 (111) 123 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats T-6295 (ppm) Mix Date Replicate 0 0.5 2 5 20 6/4/98g 6/11/98g 6/18/98a 1 b 2 b Mean - 1 b 2 b Mean - 1 b 2 b 3 - Mean - 0.618 0.610 0.614 (123)c 1.95 1.66 1.81 (90.5) 4.91 5.39 5.15 (103) 18.2 20.3 19.3 (96.5) 0.446 0.539 0.493 (98.6) 2.25 1.91 2.08 (104) 5.22 4.81 5.02 (100) 19.0 16.9 18.0 (90.0) 0.617 0.637 0.635 0.630 (126) 2.50 2.11 2.05 2.22 (111) 5.15 5.43 4.70 5.09 (102) 18.3 17.8 18.2 18.1 (90.5) 7/16/98 7/16/98d 1 b 2 b Mean - 1 - 2 - Mean - 0.564 0.519 0.542 (108) 1.60 1.90 1.75 (87.5) 3.90 17.9 4.13 17.4 4.02 (80.4) 17.7 (88.5) - - 4.76 - - - 5.41 - - - 5.09 (102) - 8/13/98 1 b 2 b Mean - 0.478 0.569 0.524 (105) 2.07 2.04 2.06 (103) 5.60 4.95 5.28 (106) 18.9 19.9 19.4 (97.0) a Results for the 0.5-, 2.0-, 5.0- and 20-ppm levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. g Reassayed due to unacceptable calibration curve for original assays. 124 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 9/10/98 9/10/98d 9/10/98e 9/10/98d,e 9/10/98f 1 b 2 b Mean - 1 - 2 - Mean - 1 - 2 - Mean - 1 - 2 - Mean - 1 - 2 - Mean - 0.477 2.28 1.38 (276)c 2.23 2.27 2.25 (113) 0.213 - 0.231 - 0.222 (44.4) - 1.09 - 1.27 - 1.18 (236) - b - b - - - 0.475 - 0.386 - 0.431 (86.2) - 4.58 4.64 4.61 (92.2) 17.1 16.8 17.0 (85.0) - - - - - - - - - - - - - - - - - - - - - - - - 10/8/98 1 b 2 b Mean - 0.836 0.482 0.659 (132) 1.79 1.83 1.81 (90.5) 5.68 4.90 5.29 (106) 17.9 17.2 17.6 (88.0) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Retention. f Reassay of the retention. 125 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 10/8/98d 1 - b - - - 2 - 0.649 (130)c - - - Mean - - - - - 10/8/98e 1 - 0.473 - - - 2 - 1.02 - - - Mean - 0.747 (149) - - - 10/8/98f 1 - 0.474 - - - 2 - 0.497 - - - Mean - 0.486 (97.2) - - - 11/5/98 12/3/98 12/3/98d 1 b 2 b Mean - 1 b 2 b Mean - 1 - 2 - Mean - 0.546 0.556 0.551 (110) b b - 0.607 0.539 0.573 (115) 1.84 2.05 1.95 (97.5) 4.33 4.45 4.39 (87.8) 16.3 15.4 15.9 (79.5) 1.65g 1.51 1.58 (79.0) 3.86 3.69 3.78 (75.6) 14.6 16.6 15.6 (78.0) 1.98 - - 1.69 - - 1.84 (92.0) - - b Below the limit of quantitation (<0.4 ppm). d Reassay. c Each value in the parentheses is the percent of theoretical. e Retention. f Reassay of the retention g Group will reassayed due to unacceptable chromatography. 126 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 12/31/98 12/31/98d 1 b 2 b Mean - 1 - 2 - Mean - 0.339 0.246 0.293 (58.6)c 1.89 1.69 1.79 (89.5) 4.42 4.71 4.57 (91.4) 19.0 19.4 19.2 (96.0) 0.442 - - - 0.422 - - - 0.432 (86.4) - - - 1/28/99 1 b e 2 b e Mean - - 1/28/99d 1 - b 2 - b Mean - - 1/28/99d 1 - b 2 - b Mean - - 1.67 2.05 1.86 (93.0) 4.71 4.92 4.82 (96.4) 17.6 17.1 17.4 (87.0) - - - - - - - - - - - - - - - - - - 2/25/99 2/25/99d 1 2 Mean 1 2 Mean 1.498 b - 0.907 0.852 0.880 (176) b 0.450 b 0.526 b 0.488 (97.6) 2.06 6.79 4.43 (222) 5.67 7.48 6.58 (132) 20.1 20.1 20.1 (101) 1.64 4.91 - 2.06 4.56 - 1.85 (92.5) 4.74 (94.8) - b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e This level was outside of the modified standard curve and will be retested. 127 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 3/25/99 3/25/99d 1 b 2 b Mean - 1 - 2 - Mean - 0.414 (82.8)c b - 1.91 2.97 2.44 (122) 1.63 0.702 1.17 (234) 2.18 1.91 2.05 (103) 5.44 5.59 5.52 (110) - 18.5 19.9 19.2 (96.0) - 4/22/99 4/22/99d 1 b 2 b Mean - 1 - 2 - Mean - b b - 0.431 0.405 0.418 (83.6) 1.97 2.04 2.01 (101) - 4.73 5.72 5.23 (105) 17.7 18.8 18.3 (91.5) 5.06 - 4.90 - 4.98 (99.6) - 5/20/99 1 b 2 b Mean - 0.582 0.531 0.557 (111) 1.76 1.68 1.72 (86.0) 4.65 4.67 4.66 (93.2) 17.8 20.1 19.0 (95.0) 6/17/99 1 b 2 b Mean - 0.402 0.455 0.429 (85.8) 1.73 2.03 1.88 (94.0) 4.46 4.81 4.64 (92.8) 19.0 18.5 18.8 (94.0) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. 128 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 7/15/99 7/15/99d 1 b 2 b Mean - 1 - 2 - Mean - 0.498 0.405 0.452 (90.4)c 1.75 23.7 12.7 (635) 5.74 4.64 5.19 (104) 18.2 18.6 18.4 (92.0) - 1.77 - - - 1.91 - - - 1.84 (92.0) - - 8/12/99 8/12/99d 9/9/99f 9/9/99g 1 b 2 b Mean - 1 - 2 - Mean - 1 e 2 e Mean - 1 b 2 b Mean - 0.455 0.544 0.500 (100) - e e - 1.27 0.607 0.939 (188) 3.15 2.16 2.66 (133) 2.13 2.12 2.13 (107) 1.80 2.33 2.07 (104) - 5.96 5.80 5.88 (118) - 4.77 5.25 5.01 (100) - 22.0 20.8 21.4 (107) - 20.5 21.0 20.8 (104) - b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Samples to be re-analyzed. f Two lowest standards dropped. g Re-test. 129 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 9/9/99g 1 - 0.407 - - - 2 - 0.406 - - - Mean - 0.407 (81.4)c - - - 10/7/99 1 b 2 b Mean - 0.515 0.541 0.528 (106) 2.22 1.98 2.10 (105) 5.90 5.64 5.77 (115) 22.1 22.9 22.5 (113) 11/4/99 1 b 2 b Mean - 0.701 0.553 0.627 (125) 1.80 1.98 1.89 (94.5) 4.31 4.23 4.27 (85.4) 21.7 20.7 21.2 (106) 12/2/99 1 b 2 b Mean - 0.479 0.487 0.483 (96.6) 2.15 2.28 2.22 (111) 5.93 5.24 5.59 (112) 22.8 23.8 23.3 (117) 12/30/99 1 b 2 b Mean - 12/30/99 g 1 - 2 - Mean - 0.453 0.469 0.461 (92.2) - 1.74 4.67 h 2.03 4.16 h 1.89 (94.5) 4.42 (88.4) - - - 23.9 - - 23.4 - - 23.7 (119) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. g Re-test. h Due to dropping high standard, this sample couldn't be quantitated. It will be retested. 130 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 1/27/00 1 b 2 b Mean - 0.455 0.424 0.440 (88.0)c 2.26 1.87 2.07 (104) 5.85 5.21 5.53 (111) 22.2 21.2 21.7 (109) 2/24/00 2/24/00g 1 b 2 b Mean - 1 - 2 - Mean - 0.390 0.405 0.398 (79.6) - 2.66 2.18 2.42 (121) 2.35 2.42 2.39 (120) 8.05 7.48 7.77 (155) 6.18 5.80 5.99 (120) 23.3 26.0 24.7 (124) 21.4 20.0 20.7 (104) 3/23/00 1 - 2 - Mean - 0.240 0.479 0.360 (72.0) 2.38 2.00 2. 19(110) 8.05 6.32 7.19 (144) 25.0 26.5 25.8 (129) Overall mean concentrations of each dose level ranged from 102.3 to 108.7. b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. g Re-test Overall Mean = Total of all means Number of means 131 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: START 1 2 3 4 5 6 7 8 9 10 11 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% MALE 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 132 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% MALE 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 133 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% MALE 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 134 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 59/60 98% 49/50 98% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% MALE 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 135 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 48 49 50 51 52 53 54 55 56 57 58 59 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% MALE 58/60 97% 48/50 96% 50/50 100% 49/50 98% 57/60 95% 40/40 100% 48/50 96% 48/50 96% 50/50 100% 47/50 94% 48/51 94% 40/40 100% 48/50 96% 48/50 96% 50/50 100% 47/50 94% 48/51 94% 40/40 100% 48/50 96% 47/50 94% 49/50 98% 47/50 94% 48/51 94% 39/40 98% 48/50 96% 47/50 94% 48/50 96% 47/50 94% 48/51 94% 39/40 98% 48/50 96% 47/50 94% 48/50 96% 47/50 94% 47/51 92% 39/40 98% 48/50 96% 47/50 94% 48/50 96% 47/50 94% 46/51 90% 39/40 98% 48/50 96% 47/50 94% 48/50 96% 47/50 94% 46/51 90% 39/40 98% 136 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 60 61 62 63 64 65 66 67 68 69 70 71 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 47/50 94% 46/50 92% 48/50 96% 47/50 94% 46/51 90% 39/40 98% 47/50 94% 45/50 90% 48/50 96% 46/50 92% 46/51 90% 39/40 98% 47/50 94% 45/50 90% 48/50 96% 46/50 92% 45/51 88% 39/40 98% 44/50 88% 45/50 90% 48/50 96% 46/50 92% 45/51 88% 39/40 98% MALE 44/50 88% 45/50 90% 48/50 96% 46/50 92% 44/51 86% 39/40 98% 44/50 88% 45/50 90% 48/50 96% 46/50 92% 44/51 86% 37/40 93% 44/50 88% 45/50 90% 48/50 96% 46/50 92% 44/51 86% 37/40 93% 44/50 88% 45/50 90% 48/50 96% 45/50 90% 44/51 86% 37/40 93% 43/50 86% 45/50 90% 48/50 96% 45/50 90% 44/51 86% 35/40 88% 43/50 86% 45/50 90% 48/50 96% 45/50 90% 44/51 86% 34/40 85% 42/50 84% 45/50 90% 48/50 96% 44/50 88% 44/51 86% 32/40 80% 42/50 84% 44/50 88% 48/50 96% 44/50 88% 44/51 86% 32/40 80% 137 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 72 73 74 75 76 77 78 79 80 81 82 83 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 41/50 82% 44/50 88% 47/50 94% 44/50 88% 43/51 84% 31/40 78% 41/50 82% 42/50 84% 47/50 94% 44/50 88% 43/51 84% 31/40 78% 40/50 80% 42/50 84% 46/50 92% 44/50 88% 42/51 82% 31/40 78% 40/50 80% 42/50 84% 46/50 92% 44/50 88% 42/51 82% 30/40 75% MALE 40/50 80% 42/50 84% 45/50 90% 44/50 88% 41/51 80% 30/40 75% 38/50 76% 42/50 84% 45/50 90% 44/50 88% 40/51 78% 29/40 73% 37/50 74% 40/50 80% 44/50 88% 44/50 88% 40/51 78% 29/40 73% 35/50 70% 37/50 74% 41/50 82% 41/50 82% 39/51 76% 29/40 73% 31/50 62% 37/50 74% 39/50 78% 40/50 80% 38/51 75% 29/40 73% 31/50 62% 36/50 72% 38/50 76% 40/50 80% 37/51 73% 28/40 70% 31/50 62% 36/50 72% 38/50 76% 40/50 80% 37/51 73% 28/40 70% 30/50 60% 35/50 70% 38/50 76% 38/50 76% 37/51 73% 25/40 63% 138 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 84 85 86 87 88 89 90 91 92 93 94 95 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 27/50 54% 32/50 64% 36/50 72% 37/50 74% 37/51 73% 25/40 63% 26/50 52% 32/50 64% 35/50 70% 37/50 74% 37/51 73% 24/40 60% 26/50 52% 30/50 60% 35/50 70% 37/50 74% 36/51 71% 24/40 60% 26/50 52% 30/50 60% 32/50 64% 36/50 72% 36/51 71% 24/40 60% MALE 25/50 50% 26/50 52% 31/50 62% 36/50 72% 35/51 69% 24/40 60% 24/50 48% 26/50 52% 31/50 62% 35/50 70% 35/51 69% 23/40 58% 24/50 48% 25/50 50% 30/50 60% 32/50 64% 35/51 69% 23/40 58% 22/50 44% 23/50 46% 29/50 58% 29/50 58% 35/51 69% 21/40 53% 22/50 44% 22/50 44% 29/50 58% 27/50 54% 35/51 69% 21/40 53% 22/50 44% 22/50 44% 28/50 56% 27/50 54% 35/51 69% 21/40 53% 21/50 42% 22/50 44% 26/50 52% 27/50 54% 33/51 65% 20/40 50% 21/50 42% 22/50 44% 23/50 46% 27/50 54% 32/51 63% 18/40 45% 139 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 96 97 98 99 100 101 102 103 104 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 19/50 38% 19/50 38% 23/50 46% 27/50 54% 32/51 63% 18/40 45% 19/50 38% 18/50 36% 21/50 42% 27/50 54% 31/51 61% 16/40 40% 17/50 34% 18/50 36% 20/50 40% 26/50 52% 30/51 59% 15/40 38% 16/50 32% 18/50 36% 19/50 38% 26/50 52% 28/51 55% 14/40 35% MALE 15/50 30% 17/50 34% 19/50 38% 26/50 52% 27/51 53% 14/40 35% 14/50 28% 14/50 28% 19/50 38% 26/50 52% 25/51 49% 13/40 33% 14/50 28% 14/50 28% 18/50 36% 26/50 52% 25/51 49% 13/40 33% 13/50 26% 13/50 26% 17/50 34% 25/50 50% 24/51 47% 11/40 27% 11/50 22% 11/50 22% 17/50 34% 25/50 50% 23/51 45% 11/40 27% 140 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: START 1 2 3 4 5 6 7 8 9 10 11 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% FEMALE 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 141 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% FEMALE 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 142 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% FEMALE 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 143 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 60/60 100% 49/50 98% 49/50 98% 49/50 98% 59/60 98% 40/40 100% FEMALE 60/60 100% 49/50 98% 49/50 98% 49/50 98% 59/60 98% 40/40 100% 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 48/50 96% 48/50 96% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 48/50 96% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 48/50 96% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 144 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 48 49 50 51 52 53 54 55 56 57 58 59 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 60/60 100% 48/50 96% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 48/50 96% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 47/50 94% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 60/60 100% 47/50 94% 47/50 94% 49/50 98% 59/60 98% 39/40 98% FEMALE 60/60 100% 47/50 94% 47/50 94% 48/50 96% 59/60 98% 39/40 98% 50/50 100% 47/50 94% 47/50 94% 48/50 96% 49/50 98% 39/40 98% 50/50 100% 47/50 94% 47/50 94% 48/50 96% 49/50 98% 39/40 98% 50/50 100% 47/50 94% 47/50 94% 48/50 96% 48/50 96% 39/40 98% 50/50 100% 47/50 94% 47/50 94% 48/50 96% 48/50 96% 39/40 98% 49/50 98% 47/50 94% 47/50 94% 48/50 96% 48/50 96% 39/40 98% 49/50 98% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 49/50 98% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 145 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 60 61 62 63 64 65 66 67 68 69 70 71 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 48/50 96% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 48/50 96% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 48/50 96% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 48/50 96% 46/50 92% 46/50 92% 48/50 96% 46/50 92% 39/40 98% FEMALE 48/50 96% 46/50 92% 46/50 92% 48/50 96% 46/50 92% 39/40 98% 47/50 94% 45/50 90% 46/50 92% 48/50 96% 46/50 92% 38/40 95% 47/50 94% 44/50 88% 42/50 84% 47/50 94% 45/50 90% 38/40 95% 47/50 94% 44/50 88% 42/50 84% 47/50 94% 45/50 90% 38/40 95% 47/50 94% 44/50 88% 42/50 84% 47/50 94% 44/50 88% 38/40 95% 47/50 94% 43/50 86% 42/50 84% 47/50 94% 44/50 88% 38/40 95% 47/50 94% 42/50 84% 41/50 82% 47/50 94% 44/50 88% 38/40 95% 47/50 94% 41/50 82% 40/50 80% 47/50 94% 44/50 88% 37/40 93% 146 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 72 73 74 75 76 77 78 79 80 81 82 83 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 45/50 90% 39/50 78% 39/50 78% 45/50 90% 44/50 88% 37/40 93% 44/50 88% 39/50 78% 39/50 78% 45/50 90% 44/50 88% 37/40 93% 44/50 88% 39/50 78% 39/50 78% 45/50 90% 44/50 88% 37/40 93% 44/50 88% 39/50 78% 38/50 76% 44/50 88% 43/50 86% 36/40 90% FEMALE 44/50 88% 39/50 78% 37/50 74% 44/50 88% 42/50 84% 35/40 88% 44/50 88% 39/50 78% 37/50 74% 41/50 82% 42/50 84% 35/40 88% 44/50 88% 39/50 78% 37/50 74% 40/50 80% 41/50 82% 35/40 88% 41/50 82% 39/50 78% 37/50 74% 40/50 80% 40/50 80% 35/40 88% 41/50 82% 39/50 78% 35/50 70% 39/50 78% 40/50 80% 35/40 88% 39/50 78% 39/50 78% 33/50 66% 39/50 78% 40/50 80% 35/40 88% 39/50 78% 38/50 76% 31/50 62% 39/50 78% 40/50 80% 35/40 88% 37/50 74% 38/50 76% 28/50 56% 38/50 76% 40/50 80% 34/40 85% 147 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 84 85 86 87 88 89 90 91 92 93 94 95 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 37/50 74% 37/50 74% 26/50 52% 37/50 74% 40/50 80% 32/40 80% 35/50 70% 37/50 74% 25/50 50% 37/50 74% 40/50 80% 32/40 80% 35/50 70% 37/50 74% 23/50 46% 37/50 74% 39/50 78% 31/40 78% 34/50 68% 35/50 70% 23/50 46% 37/50 74% 39/50 78% 30/40 75% FEMALE 33/50 66% 34/50 68% 23/50 46% 37/50 74% 38/50 76% 29/40 73% 31/50 62% 32/50 64% 23/50 46% 35/50 70% 38/50 76% 29/40 73% 31/50 62% 31/50 62% 22/50 44% 33/50 66% 38/50 76% 27/40 68% 31/50 62% 31/50 62% 19/50 38% 33/50 66% 37/50 74% 25/40 63% 31/50 62% 29/50 58% 16/50 32% 33/50 66% 33/50 66% 23/40 58% 28/50 56% 29/50 58% 16/50 32% 31/50 62% 32/50 64% 23/40 58% 28/50 56% 26/50 52% 16/50 32% 29/50 58% 31/50 62% 23/40 58% 28/50 56% 26/50 52% 15/50 30% 28/50 56% 31/50 62% 23/40 58% 148 TABLE 4 Survival Data Covance 6329-183 3M T-6295 PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 96 97 98 99 100 101 102 103 104 ---------------------------------------------------------------------------------------------------------------------- 1 0 2 .500 3 2 4 5 5 20 6 20 26/50 52% 25/50 50% 14/50 28% 25/50 50% 30/50 60% 22/40 55% 26/50 52% 24/50 48% 13/50 26% 24/50 48% 30/50 60% 22/40 55% 26/50 52% 21/50 42% 12/50 24% 23/50 46% 30/50 60% 21/40 53% 26/50 52% 19/50 38% 12/50 24% 20/50 40% 30/50 60% 21/40 53% FEMALE 26/50 52% 19/50 38% 12/50 24% 19/50 38% 27/50 54% 21/40 53% 25/50 50% 18/50 36% 11/50 22% 18/50 36% 27/50 54% 21/40 53% 25/50 50% 16/50 32% 10/50 20% 18/50 36% 26/50 52% 21/40 53% 25/50 50% 15/50 30% 17/50 34% 26/50 52% 21/40 53% 25/50 50% 15/50 30% 17/50 34% 26/50 52% 19/40 48% 149 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** APPEARANCE BENT TAIL 010001 CONVULSIONS 000101 HEAD TILT LEFT 010101 RIGHT 401002 HUNCHED 333222 LIMITED USE LIMB-FRONT-LEFT 010000 LIMB-HIND-LEFT 000010 LIMB-HIND-RIGHT 200010 LIMBS-ALL 100011 LIMBS-HIND 040122 PAW-HIND-LEFT 000011 PAW-HIND-RIGHT 100000 PAWS-ALL 000010 PAWS-FRONT 010000 PAWS-HIND 100000 MALOCCLUSION 12 7 9 9 11 10 MISSING TAIL-DISTAL 000000 TEETH 251152 OBES 110132 PARALYSIS LIMBS-HIND 010000 150 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ APPEARANCE PROLAPSED VAGINA SWOLLEN DORSAL DIGIT(S)-HIND-LEFT DIGIT(S)-HIND-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PERI-ORAL PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND TAIL TAIL-DISTAL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VAGINA TESTES TESTIS-LEFT 000000 000000 000000 100000 002012 100002 000010 010000 100001 001000 000100 132015 402234 100010 100000 000000 001000 100300 000010 000000 001000 000001 151 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ APPEARANCE THIN TREMORS LIMBS-ALL ENTIRE BODY HEAD 15 21 17 10 12 11 100000 000010 010000 BEHAVIOR AGGRESSIVE <UNSPECIFIED> ATAXIC <UNSPECIFIED> MODERATE CIRCLING LEFT RIGHT HYPOACTIVE <UNSPECIFIED> MILD MODERATE SEVERE RECUMBENT DORSAL LATERAL STERNAL 001000 440112 000000 000000 000000 10 13 14 11 10 10 000000 100001 010000 000001 432202 110012 152 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ BEHAVIOR SENSITIVE TO TOUCH COLD TO TOUCH LIMBS-ALL LIMBS-HIND TAIL ENTIRE BODY 010200 000011 010000 000010 241324 DISCHARGE GENITAL RED IN COLOR CLEAR ORAL <UNSPECIFIED> MILD MODERATE NASAL RED IN COLOR UNKNOWN RED IN COLOR 612241 100001 000100 000000 201202 002000 EXCRETION DISCOLORED FECES RED IN COLOR YELLOW IN COLOR 100000 000000 153 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ EXCRETION DISCOLORED URINE RED IN COLOR FEW FECES LIQUID FECES NO FECES NONFORMED FECES 001000 16 23 16 16 18 16 000101 623534 232324 EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT PALE EYE-RIGHT EYES PROTRUDING EYE-LEFT 121000 010100 020000 001000 000000 000200 000100 001000 000000 010200 001000 154 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ EYES PROTRUDING EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-LEFT EYE-RIGHT SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT 000000 000010 225112 122633 553523 001000 020000 703222 001200 RESPIRATION AUDIBLE IRREGULAR LABORED 531113 621113 242122 SKIN & PELAGE ALOPECIA BROWN HAIR COAT PERINEAL AREA SCROTUM 000000 100210 110001 155 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL BLUE SKIN PAWS-ALL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-LEFT BROWN SKIN VENTRAL-ABDOMINAL BLACK SKIN DORSAL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT PALE ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL HEAD-ENTIRE HEAD-CRANIAL 010011 000000 000000 000000 000000 000000 000000 000110 000000 000010 010000 000000 000000 000000 000000 055312 000000 543333 000000 156 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LATERA-RIGHT LATERAL-THORACIC-LEFT PERINEAL AREA SHOULDER-RIGHT VENTRAL-ABDOMINAL ROUGH HAIR COAT RED SKIN EARS HEAD-MAXILLARY-RIGHT PAW-HIND-RIGHT SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT 000100 000100 000100 000000 000000 000000 000000 000010 201110 766434 000000 100000 000001 000120 000001 370442 010000 001010 000000 011100 000020 010001 157 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB EAR-LEFT EAR-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LIMB-FRONT-RIGHT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-LEFT LATERAL-THORACIC-LEFT MOUTH PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-DISTAL TAIL-MID TAIL-PROXIMAL 001000 000001 010010 001000 000010 100100 001010 001000 001000 000210 000000 000000 000100 000000 000101 221322 102312 100201 011000 211001 000000 000000 001000 200000 158 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT WARM TO TOUCH LIMBS-HIND YELLOW HAIR COAT YELLOW SKIN PAWS-ALL TAIL BLACK HAIR COAT - PERINEAL COLD TO TOUCH 214265 032232 043481 020000 000000 000000 000000 000000 000000 000000 420120 000000 000000 000100 000001 159 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** APPEARANCE BENT TAIL 000000 CONVULSIONS 101012 HEAD TILT LEFT 547623 RIGHT 235521 HUNCHED 345653 LIMITED USE LIMB-FRONT-LEFT 000000 LIMB-HIND-LEFT 110000 LIMB-HIND-RIGHT 000000 LIMBS-ALL 000000 LIMBS-HIND 000000 PAW-HIND-LEFT 000000 PAW-HIND-RIGHT 000000 PAWS-ALL 000000 PAWS-FRONT 000000 PAWS-HIND 000000 MALOCCLUSION 16 13 11 12 10 8 MISSING TAIL-DISTAL 101100 TEETH 252351 OBES 202000 PARALYSIS LIMBS-HIND 000000 160 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ APPEARANCE PROLAPSED VAGINA SWOLLEN DORSAL DIGIT(S)-HIND-LEFT DIGIT(S)-HIND-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PERI-ORAL PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND TAIL TAIL-DISTAL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VAGINA TESTES TESTIS-LEFT 011000 000010 100000 000000 100010 000100 000000 010100 000200 000000 000000 101020 111000 000000 000000 001000 000100 100020 000000 001000 000000 000000 161 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ APPEARANCE THIN TREMORS LIMBS-ALL ENTIRE BODY HEAD 12 14 21 17 17 9 000000 001023 000000 BEHAVIOR AGGRESSIVE <UNSPECIFIED> ATAXIC <UNSPECIFIED> MODERATE CIRCLING LEFT RIGHT HYPOACTIVE <UNSPECIFIED> MILD MODERATE SEVERE RECUMBENT DORSAL LATERAL STERNAL 000000 649633 001000 001000 100000 8 7 16 15 7 10 001000 012010 001000 001000 312031 000001 162 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ BEHAVIOR SENSITIVE TO TOUCH COLD TO TOUCH LIMBS-ALL LIMBS-HIND TAIL ENTIRE BODY 100000 000100 000000 000000 021231 DISCHARGE GENITAL RED IN COLOR CLEAR ORAL <UNSPECIFIED> MILD MODERATE NASAL RED IN COLOR UNKNOWN RED IN COLOR AXILLARY LEFT MASS BLEEDING 032111 001010 000000 001000 423142 000000 000100 EXCRETION DISCOLORED FECES RED IN COLOR YELLOW IN COLOR 000000 000010 163 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ EXCRETION DISCOLORED URINE RED IN COLOR FEW FECES LIQUID FECES NO FECES NONFORMED FECES 000000 17 20 20 16 13 10 000000 677232 211101 EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT PALE EYE-RIGHT EYES PROTRUDING EYE-LEFT 201110 211020 001010 010110 102000 100010 100010 000001 000010 011200 000000 164 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ EYES PROTRUDING EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-LEFT EYE-RIGHT SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT SWOLLEN RIGHT EYELID 000010 341060 115332 561542 7 5 14 7 7 6 001000 000020 101000 003030 001000 RESPIRATION AUDIBLE IRREGULAR LABORED 105212 113011 121111 SKIN & PELAGE ALOPECIA BROWN HAIR COAT PERINEAL AREA SCROTUM 200231 201010 000000 165 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL BLUE SKIN PAWS-ALL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-LEFT BROWN SKIN VENTRAL-ABDOMINAL BLACK SKIN DORSAL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT PALE ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL HEAD-ENTIRE HEAD-CRANIAL 020100 010000 010000 100000 020020 001000 001000 010000 100000 100000 000121 020001 010000 100000 001100 121322 002000 459464 012000 166 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LATERA-RIGHT LATERAL-THORACIC-LEFT PERINEAL AREA SHOULDER-RIGHT VENTRAL-ABDOMINAL ROUGH HAIR COAT RED SKIN EARS HEAD-MAXILLARY-RIGHT PAW-HIND-RIGHT SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT 000000 000000 000000 000100 001000 001000 000010 000000 001201 465254 001000 000000 000000 213112 111100 110010 012011 000010 001010 100000 000000 301000 167 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB EAR-LEFT EAR-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LIMB-FRONT-RIGHT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-LEFT LATERAL-THORACIC-LEFT MOUTH PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-DISTAL TAIL-MID TAIL-PROXIMAL 000000 000000 001010 001000 000100 001010 000100 000100 010000 000000 000100 000002 000000 100000 220031 112254 142221 111141 100000 010010 001000 000100 000000 000100 168 Table 5 Covance 6329-183 3M T-6295 Summary of Clinical Observations PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT WARM TO TOUCH LIMBS-HIND YELLOW HAIR COAT YELLOW SKIN PAWS-ALL TAIL *** END OF LIST *** 300130 132021 120213 000010 111000 000110 110001 102200 000001 000001 225323 000100 000100 169 Table 6 Covance 6329-183 3M T-6295 Summary of Mass Observations PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT 221130 AXILLARY REGION-RIGHT 020002 DORSAL 3 10 4 8 6 3 DORSAL-CERVICAL-LEFT 001010 DORSAL-CERVICAL-RIGHT 000001 EAR-LEFT 001000 EAR-RIGHT 000001 HEAD-MAXILLARY-LEFT 011101 HEAD-MAXILLARY-RIGHT 102100 INGUINAL REGION-LEFT 110133 INGUINAL REGION-RIGHT 010230 LIMB-FRONT-LEFT 100000 LIMB-FRONT-RIGHT 000200 LIMB-HIND-LEFT 000000 LIMB-HIND-RIGHT 010000 MOUTH 000200 PAW-FRONT-LEFT 000010 PAW-HIND-RIGHT 000100 SHOULDER-LEFT 011000 SHOULDER-RIGHT 120001 TAIL 001000 TAIL-MID 001000 TAIL-PROXIMAL 601000 VENTRAL-ABDOMINAL 332462 170 Table 6 Covance 6329-183 3M T-6295 Summary of Mass Observations PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT RECTUM 263472 054463 010000 101000 000000 100000 000100 001000 000010 WART LIKE LESION(S) AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT EAR-RIGHT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LIMB-FRONT-RIGHT MOUTH NOSE PAW-HIND-LEFT 000100 000001 221204 000000 000100 100000 000001 022000 100000 001100 000010 110000 100000 171 Table 6 Covance 6329-183 3M T-6295 Summary of Mass Observations PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: ------------MALE------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-MID TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-THORACIC-LEFT 001000 000001 000001 000100 000000 000101 000001 000001 001001 172 Table 6 Covance 6329-183 3M T-6295 Summary of Mass Observations PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT 15 11 15 15 8 6 AXILLARY REGION-RIGHT 11 7 11 13 7 5 DORSAL 022020 DORSAL-CERVICAL-LEFT 000000 DORSAL-CERVICAL-RIGHT 000000 EAR-LEFT 000000 EAR-RIGHT 000001 HEAD-MAXILLARY-LEFT 011120 HEAD-MAXILLARY-RIGHT 100100 INGUINAL REGION-LEFT 8 10 10 12 8 7 INGUINAL REGION-RIGHT 9 7 10 4 9 3 LIMB-FRONT-LEFT 000100 LIMB-FRONT-RIGHT 000000 LIMB-HIND-LEFT 100100 LIMB-HIND-RIGHT 010100 MOUTH 000000 PAW-FRONT-LEFT 000000 PAW-HIND-RIGHT 000000 SHOULDER-LEFT 000120 SHOULDER-RIGHT 020010 TAIL 000000 TAIL-MID 000000 TAIL-PROXIMAL 000100 VENTRAL-ABDOMINAL 12 7 6 5 4 1 173 Table 6 Covance 6329-183 3M T-6295 Summary of Mass Observations PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ MASS(ES) VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT RECTUM 578994 11 10 8 6 8 5 235411 213530 041500 201020 320021 110112 300000 WART LIKE LESION(S) AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT EAR-RIGHT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LIMB-FRONT-RIGHT MOUTH NOSE PAW-HIND-LEFT 000000 000000 000000 001000 000000 000000 000000 000001 000001 000000 400011 010010 000000 174 Table 6 Covance 6329-183 3M T-6295 Summary of Mass Observations PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-736 SEX: -----------FEMALE----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------------------------------ WART LIKE LESION(S) SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-MID TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-THORACIC-LEFT *** END OF LIST *** 000000 000000 001000 000000 000001 001000 000000 000000 000000 175 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 1 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 70 MEAN 179 S.D. 18.6 60 178 18.7 60 179 19.0 60 179 19.2 70 178 18.7 40 178 19.0 2 N 70 MEAN 237 S.D. 21.8 60 237 20.2 60 237 21.5 60 236 20.5 70 234 20.2 40 231 22.0 3 N 70 MEAN 287 S.D. 25.0 60 290 21.4 60 286 23.0 60 290 22.8 70 285 23.2 40 286 25.2 4 N 70 MEAN 334 S.D. 27.6 60 333 23.9 60 328 25.4 60 333 24.3 70 323 25.0 40 326 28.0 5 N 65 MEAN 367 S.D. 31.4 55 370 27.1 55 367 28.4 55 367 27.9 65 361 28.1 40 363 31.4 6 N 65 MEAN 399 S.D. 34.3 55 402 30.8 55 397 30.3 55 399 30.4 65 390 30.2 40 394 34.6 7 N 65 MEAN 419 S.D. 35.1 55 428 33.3 55 418 31.8 55 425 32.2 65 413 32.7 40 415 36.8 176 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 2 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 65 MEAN 448 S.D. 37.8 55 450 35.7 55 443 35.9 55 448 35.1 65 432 34.2 40 436 40.8 9 N 65 MEAN 464 S.D. 41.6 55 463 37.7 55 450 38.2 55 466 36.8 65 446* 36.4 40 446 41.0 10 N 65 MEAN 481 S.D. 43.1 55 483 39.8 55 473 40.7 55 479 36.9 65 465 37.2 40 466 42.2 11 N 65 MEAN 499 S.D. 45.2 55 502 42.1 55 479* 42.6 55 494 38.5 65 474* 38.5 40 473* 45.2 12 N 65 MEAN 509 S.D. 46.8 55 512 43.9 55 494 44.3 55 507 40.4 65 485* 41.5 40 483* 46.4 13 N 65 MEAN 517 S.D. 48.0 55 518 45.6 55 508 47.5 55 514 42.5 65 493* 43.2 40 491* 49.1 14 N 65 MEAN 528 S.D. 51.1 55 534 47.3 55 517 49.4 55 527 44.4 65 504* 46.1 40 501* 50.4 177 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 3 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 60 MEAN 535 S.D. 51.9 50 542 48.2 50 531 49.0 50 535 45.5 60 513* 47.5 40 510* 51.2 16 N 60 MEAN 549 S.D. 53.5 50 553 49.9 50 542 50.0 50 550 46.5 60 523* 49.3 40 517* 52.3 17 N 60 MEAN 556 S.D. 55.4 50 559 50.5 50 551 51.0 50 557 47.8 60 526* 49.8 40 521* 52.9 21 N 60 MEAN 580 S.D. 62.1 49 594 57.5 50 582 57.5 50 581 53.2 60 546* 57.2 40 544* 59.2 25 N 59 MEAN 609 S.D. 67.8 49 621 63.0 50 611 61.0 50 604 56.7 60 567* 62.9 40 566* 65.8 29 N 59 MEAN 644 S.D. 76.0 49 654 66.6 50 640 67.4 50 632 62.5 60 595* 69.0 40 595* 69.8 33 N 59 MEAN 669 S.D. 79.5 49 683 71.0 50 666 70.4 50 653 68.8 60 619* 76.6 40 620* 75.1 178 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 4 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 37 N 59 MEAN 691 S.D. 83.5 49 700 77.8 50 687 73.8 50 676 73.6 59 644* 84.7 40 644* 81.8 41 N 59 MEAN 699 S.D. 87.9 48 716 81.8 50 702 78.2 50 690 77.6 59 663 92.5 40 657 89.6 45 N 59 MEAN 715 S.D. 95.8 48 735 87.8 50 721 80.4 50 709 82.3 59 676 100.7 40 674 97.6 49 N 58 MEAN 736 S.D. 97.2 48 759 89.5 50 740 83.5 50 726 88.9 58 699 108.3 40 697 104.3 53 N 58 MEAN 749 S.D. 103.5 48 770 91.7 50 753 86.2 48 742 97.1 57 713 115.0 40 712 109.2 57 N 48 MEAN 757 S.D. 109.7 47 781 95.6 48 761 88.0 47 753 100.2 47 722 116.0 39 734 118.5 61 N 47 MEAN 764 S.D. 122.9 46 790 97.0 48 777 91.3 47 768 106.4 46 742 127.6 39 742 123.0 179 Table 7 Covance 6329-183 3M T-6295 Summary of Body Weight Data (g) PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 65 N 44 MEAN 786 S.D. 115.1 45 795 104.4 48 783 94.7 46 782 109.5 44 765 123.9 39 740 133.4 69 N 43 MEAN 799 S.D. 120.1 45 791 108.1 48 784 105.6 45 794 113.0 44 772 127.4 35 756 151.3 73 N 41 MEAN 811 S.D. 113.7 44 790 116.1 47 790 115.1 44 804 115.4 43 779 145.1 31 801 145.7 77 N 40 MEAN 789 S.D. 124.1 42 772 114.2 45 773 119.1 44 799 125.0 41 783 142.4 30 797 152.8 81 N 31 MEAN 811 S.D. 124.9 37 774 111.7 39 779 132.6 40 799 124.4 38 785 121.9 29 788 153.3 85 N 27 MEAN 835 S.D. 115.7 32 762 114.7 36 781 138.8 37 772 117.3 37 790 128.7 25 795 150.0 89 N 25 MEAN 812 S.D. 137.1 26 760 125.1 31 795 129.9 36 759 136.4 35 796 136.1 24 782 140.4 180 Table 7 Covance 6329-183 3M T-6295 Summary of Body Weight Data (g) PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 93 N 22 MEAN 825 S.D. 128.2 22 786 109.6 29 809 123.8 27 821 112.4 35 799 129.9 21 767 133.1 97 N 19 MEAN 772 S.D. 132.2 19 761 105.3 23 789 157.6 27 814 116.2 32 769 140.8 18 737 155.9 101 N MEAN S.D. 15 755 159.1 17 717 123.5 19 814 138.3 26 802 124.6 27 765 122.3 14 754 127.6 105 N MEAN S.D. 11 746 164.5 11 734 154.1 17 809 129.6 25 768 130.4 23 755 136.1 11 719 151.1 181 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 7 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 70 MEAN 154 S.D. 11.1 60 154 10.4 60 155 10.0 60 154 10.1 70 153 11.1 40 155 11.8 2 N 70 MEAN 183 S.D. 14.6 60 180 15.0 60 176 14.2 60 180 13.2 70 177 13.5 40 178 14.8 3 N 70 MEAN 207 S.D. 18.6 60 204 17.4 60 199* 15.7 60 204 15.5 70 199* 16.2 40 198* 15.8 4 N 70 MEAN 224 S.D. 22.1 60 222 21.2 60 217 18.9 60 218 19.1 70 215* 17.4 40 214 18.8 5 N 65 MEAN 237 S.D. 23.6 55 236 22.9 55 232 20.1 55 232 20.0 65 224* 17.4 40 227 19.4 6 N 65 MEAN 248 S.D. 25.3 55 250 25.0 55 245 21.6 55 244 21.1 65 233* 19.6 40 235* 19.8 7 N 65 MEAN 254 S.D. 27.7 55 257 27.1 55 251 20.0 55 250 21.7 65 236* 20.1 40 240* 18.8 182 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 8 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 65 MEAN 266 S.D. 30.0 55 269 28.1 55 262 23.0 55 263 24.4 65 247* 20.2 40 250* 21.0 9 N 65 MEAN 268 S.D. 31.4 55 273 30.2 55 263 21.7 55 264 25.3 65 245* 22.0 40 247* 22.1 10 N 65 MEAN 279 S.D. 33.5 55 283 31.7 54 271 23.7 55 275 27.2 65 253* 22.4 40 254* 22.0 11 N 65 MEAN 281 S.D. 33.8 55 291 33.1 54 277 23.8 55 277 25.8 65 258* 22.6 40 255* 22.6 12 N 65 MEAN 290 S.D. 37.0 55 292 34.5 54 278 23.6 55 279 27.5 65 257* 21.1 40 259* 23.0 13 N 65 MEAN 295 S.D. 38.9 55 296 35.3 54 284 24.4 55 285 29.1 65 261* 22.7 40 262* 23.2 14 N 65 MEAN 300 S.D. 40.1 55 303 37.6 54 292 24.5 55 292 31.7 65 265* 23.3 40 266* 23.6 183 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 9 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 60 MEAN 302 S.D. 42.2 50 304 36.7 49 294 26.4 50 298 33.3 60 266* 22.0 40 269* 23.7 16 N 60 MEAN 307 S.D. 43.9 50 310 39.1 49 298 28.2 50 303 33.8 60 268* 23.1 40 271* 24.3 17 N 60 MEAN 307 S.D. 43.9 50 311 38.3 49 303 26.5 50 305 34.9 60 272* 23.6 40 273* 25.0 21 N 60 MEAN 316 S.D. 44.8 50 322 40.2 49 314 29.9 50 313 38.2 60 280* 24.1 40 280* 26.5 25 N 60 MEAN 331 S.D. 50.9 50 335 45.6 49 324 30.7 50 328 42.2 60 290* 27.8 40 291* 29.6 29 N 60 MEAN 342 S.D. 52.8 50 347 50.0 49 338 36.1 50 342 47.2 60 298* 30.2 40 301* 32.5 33 N 60 MEAN 358 S.D. 58.0 50 362 55.0 49 356 40.7 49 353 46.8 60 310* 32.8 40 312* 36.8 184 Table 7 Summary of Body Weight Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 10 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 37 N 60 MEAN 373 S.D. 64.8 49 375 56.7 49 371 43.4 49 365 47.3 60 319* 36.6 40 321* 39.8 41 N 60 MEAN 389 S.D. 69.4 49 393 62.4 49 385 50.0 49 378 49.8 59 328* 38.3 40 329* 46.5 45 N 60 MEAN 409 S.D. 73.3 48 413 63.6 49 396 59.8 49 394 51.1 59 339* 41.0 39 346* 48.0 49 N 60 MEAN 422 S.D. 74.5 48 424 66.3 47 415 55.7 49 407 55.1 59 351* 45.5 39 356* 50.0 53 N 60 MEAN 427 S.D. 78.8 47 435 71.8 47 428 61.2 48 422 56.5 59 358* 48.3 39 366* 52.6 57 N 50 MEAN 440 S.D. 79.8 47 451 77.9 47 447 68.4 48 443 59.0 48 371* 54.6 39 393* 59.4 61 N 48 MEAN 463 S.D. 81.3 47 464 83.4 47 463 75.4 48 458 59.3 47 382* 58.8 39 419* 63.9 185 Table 7 Covance 6329-183 3M T-6295 Summary of Body Weight Data (g) PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 65 N 47 MEAN 472 S.D. 86.7 46 473 93.5 46 469 85.5 48 458 63.6 46 384* 67.7 39 433 73.3 69 N 47 MEAN 486 S.D. 92.2 43 496 93.6 42 492 85.2 47 475 66.0 44 397* 63.0 38 460 76.4 73 N 45 MEAN 494 S.D. 98.0 39 508 89.0 39 504 93.6 45 478 78.0 44 400* 68.3 37 479 76.9 77 N 44 MEAN 499 S.D. 103.3 39 519 93.7 37 509 104.3 44 477 78.3 42 403* 78.3 35 486 79.5 81 N 41 MEAN 514 S.D. 100.3 39 526 100.9 35 503 118.8 39 485 79.8 40 417* 70.3 35 490 84.4 85 N 37 MEAN 512 S.D. 107.0 37 537 98.0 26 525 128.8 37 493 83.4 40 423* 76.1 32 500 98.9 89 N 32 MEAN 516 S.D. 89.6 34 550 110.6 23 523 120.9 37 496 81.3 38 431* 74.5 29 524 96.4 186 Table 7 Covance 6329-183 3M T-6295 Summary of Body Weight Data (g) PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 93 N 31 MEAN 504 S.D. 99.4 29 550 114.7 16 565 125.9 33 509 72.3 33 438* 77.5 23 548 92.5 97 N 26 MEAN 524 S.D. 93.1 25 556 103.8 14 572 139.3 25 518 77.9 30 451* 85.9 22 555 97.9 101 N MEAN S.D. 26 528 97.2 19 541 96.5 12 566 151.0 19 529 78.9 27 452* 87.3 21 536 116.5 105 N 24 15 0 17 26 19 MEAN 516 523 518 447 542 S.D. 105.7 117.0 85.6 101.2 138.4 187 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 1 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1-2 N MEAN S.D. 70 58 5.8 60 59 5.6 60 58 5.2 60 57 4.4 70 57 5.9 40 53* 6.4 2-3 N MEAN S.D. 70 50 6.5 60 53* 6.2 60 48 6.2 60 55* 7.5 70 50 6.2 40 56* 7.2 3-4 N MEAN S.D. 70 47 6.8 60 42* 8.0 60 43* 7.6 60 43* 6.8 70 38* 6.6 40 40* 7.3 4-5 N MEAN S.D. 65 34 7.6 55 38* 6.3 55 38* 6.8 55 34 8.2 65 37 5.6 40 37 5.8 5-6 N MEAN S.D. 65 32 6.2 55 32 7.0 55 30 5.7 55 32 5.3 65 29 5.1 40 30 6.0 6-7 N MEAN S.D. 65 20 6.5 55 26* 5.9 55 21 6.3 55 26* 5.8 65 23 7.5 40 21 5.3 7-8 N MEAN S.D. 65 29 5.8 55 22* 6.7 55 25* 7.2 55 23* 6.7 65 20* 5.5 40 21* 10.4 188 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 2 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8-9 N MEAN S.D. 65 17 11.0 55 13 6.1 55 7* 5.8 55 18 4.7 65 13 7.9 40 9* 9.0 9-10 N MEAN S.D. 65 16 5.7 55 20* 4.7 55 23* 6.5 55 13* 4.0 65 20* 5.3 40 20* 5.0 10-11 N MEAN S.D. 65 18 5.3 55 19 5.1 55 6* 6.9 55 15* 6.8 65 9* 6.5 40 8* 5.8 11-12 N MEAN S.D. 65 10 8.2 55 10 4.2 55 15* 9.3 55 13* 6.0 65 10 5.6 40 10 4.3 12-13 N MEAN S.D. 65 9 5.8 55 6* 5.5 55 14* 6.8 55 7 5.1 65 8 4.7 40 8 5.4 13-14 N MEAN S.D. 65 11 5.4 55 16* 4.7 55 9 4.4 55 13 4.3 65 11 7.1 40 10 5.8 14-15 N MEAN S.D. 60 7 5.8 50 6 6.5 50 9 4.7 50 11* 5.7 60 8 4.5 40 9 5.1 189 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 3 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15-16 N MEAN S.D. 60 14 4.7 50 11* 5.6 50 11* 4.7 50 14 3.9 60 10* 4.6 40 8* 4.6 16-17 N MEAN S.D. 60 7 5.3 50 7 4.6 50 9 4.6 50 8 4.2 60 3* 5.5 40 4* 4.7 17-21 N MEAN S.D. 60 25 12.0 49 34* 10.5 50 30* 11.3 50 23 11.6 60 20 11.4 40 22 10.4 21-25 N MEAN S.D. 59 28 9.9 49 27 12.9 50 30 10.5 50 23 9.9 60 21* 9.9 40 22* 12.1 25-29 N MEAN S.D. 59 35 13.0 49 34 13.5 50 29* 10.3 50 28* 9.6 60 28* 11.7 40 30 7.7 29-33 N MEAN S.D. 59 26 10.2 49 29 10.7 50 25 8.9 50 20* 12.1 60 24 12.4 40 25 9.7 33-37 N MEAN S.D. 59 21 10.3 49 17 23.1 50 21 13.0 50 23 9.8 59 26 11.9 40 24 11.8 190 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 4 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 37-41 N MEAN S.D. 59 8 12.5 48 13 11.9 50 15* 10.6 50 15* 14.2 59 19* 11.7 40 13 11.4 41-45 N MEAN S.D. 59 16 19.8 48 19 10.2 50 19 8.6 50 19 12.4 59 13 14.1 40 16 10.9 45-49 N MEAN S.D. 58 20 15.6 48 24 8.9 50 19 9.7 50 17 12.9 58 21 13.8 40 24 9.6 49-53 N MEAN S.D. 58 13 15.9 48 11 15.0 50 13 8.9 48 12 28.3 57 14 13.2 40 15 10.3 53-57 N MEAN S.D. 48 10 20.8 47 12 14.5 48 9 9.7 47 7 13.1 47 13 21.4 39 22* 17.2 57-61 N MEAN S.D. 47 8 37.4 46 4 26.6 48 16 15.0 47 15 22.9 46 16 21.1 39 8 32.3 61-65 N MEAN S.D. 44 6 25.5 45 6 18.3 48 7 18.7 46 12 23.9 44 14 9.3 39 -2 60.5 191 Table 8 Covance 6329-183 3M T-6295 Summary of Body Weight Change Data (g) PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 65-69 N MEAN S.D. 43 10 21.8 45 -4 41.6 48 1 37.2 45 10 19.4 44 7 25.4 35 3 43.2 69-73 N MEAN S.D. 41 6 17.8 44 -6 46.7 47 0 34.4 44 8 19.2 43 6* 49.1 31 19* 25.4 73-77 N MEAN S.D. 40 -14 47.0 42 -32* 58.0 45 -28* 53.4 44 -5 19.0 41 -7 33.0 30 -8 33.8 77-81 N MEAN S.D. 31 -8 34.0 37 -18 43.3 39 -10 40.2 40 -2 31.0 38 -8 32.9 29 -3 45.3 81-85 N MEAN S.D. 27 -1 30.9 32 -24 62.6 36 -14 52.7 37 -13 45.0 37 5 32.6 25 -15 36.3 85-89 N MEAN S.D. 25 -23 46.0 26 -14 48.6 31 -3* 29.5 36 -17* 59.8 35 3* 23.6 24 -21 39.0 89-93 N MEAN S.D. 22 -1 26.4 22 -1 30.2 29 -7 43.7 27 15 13.3 35 3 48.7 21 -2 52.7 192 Table 8 Covance 6329-183 3M T-6295 Summary of Body Weight Change Data (g) PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 93-97 N MEAN S.D. 19 -28 58.1 19 -33 51.8 23 -10 42.4 27 -6 31.1 32 -17 55.1 18 -31 71.1 97-101 N MEAN S.D. 15 -29 47.9 17 -55 85.0 19 -6 41.4 26 -10 32.7 27 -13 52.5 14 -32 42.4 101-105 N MEAN S.D. 11 -21 44.8 11 -30 68.4 17 -23 44.5 25 -33 64.6 23 -21 41.4 11 -39 64.3 1-15 N MEAN S.D. 60 356 45.5 50 364 46.3 50 352 44.0 50 356 42.0 60 334* 44.0 40 332* 47.7 15-29 N MEAN S.D. 59 109 31.4 49 112 25.5 50 109 24.8 50 97 23.5 60 82* 30.8 40 86* 23.9 29-57 N MEAN S.D. 48 114 53.7 47 128 42.5 48 120 32.0 47 119 57.5 47 131 62.9 39 138 55.2 57-105 N MEAN S.D. 11 13 123.1 11 2 170.9 17 70 80.5 25 39 100.6 23 70 106.5 11 9 116.3 1-105 N MEAN S.D. 11 562 157.0 11 560 160.7 17 631 128.1 25 588 128.3 23 580 137.6 11 546 147.3 193 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 7 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1-2 N MEAN S.D. 70 29 6.7 60 26* 7.5 60 21* 6.9 60 26* 6.3 70 25* 7.2 40 23* 6.1 2-3 N MEAN S.D. 70 24 7.0 60 24 6.6 60 23 6.3 60 24 6.5 70 22 7.0 40 20* 7.4 3-4 N MEAN S.D. 70 17 7.8 60 18 7.0 60 18 6.9 60 14 7.3 70 16 7.6 40 16 8.2 4-5 N MEAN S.D. 65 14 7.2 55 14 7.9 55 15 8.2 55 15 7.8 65 9* 7.4 40 13 7.2 5-6 N MEAN S.D. 65 11 6.5 55 14 6.3 55 13 5.4 55 12 6.9 65 9 5.7 40 8* 5.8 6-7 N MEAN S.D. 65 6 5.8 55 6 7.4 55 6 5.9 55 6 7.3 65 3* 5.0 40 5 6.0 7-8 N MEAN S.D. 65 13 6.8 55 12 5.0 55 11 7.4 55 13 6.0 65 11 4.9 40 10 5.1 194 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 8 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8-9 N MEAN S.D. 65 2 6.0 55 4 6.2 55 2 4.9 55 1 6.2 65 -2* 4.6 40 -3* 4.6 9-10 N MEAN S.D. 65 11 6.3 55 10 6.8 54 8* 5.8 55 11 6.8 65 8* 6.6 40 7* 5.5 10-11 N MEAN S.D. 65 2 5.1 55 8* 5.7 54 6* 5.8 55 2 5.7 65 5* 5.6 40 0 4.9 11-12 N MEAN S.D. 65 9 6.3 55 1* 6.6 54 1* 5.7 55 2* 6.0 65 -1* 5.8 40 4* 4.6 12-13 N MEAN S.D. 65 5 7.0 55 4 5.3 54 7 5.9 55 6 4.8 65 3 5.3 40 3 5.0 13-14 N MEAN S.D. 65 5 7.0 55 7 5.3 54 8* 5.1 55 7 5.5 65 4 5.1 40 5 4.3 14-15 N MEAN S.D. 60 4 5.8 50 3 6.5 49 2 6.0 50 4 6.4 60 1 5.4 40 2 3.8 195 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 9 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15-16 N MEAN S.D. 60 4 4.4 50 6 5.3 49 4 5.7 50 5 5.6 60 3 5.3 40 3 4.1 16-17 N MEAN S.D. 60 0 6.5 50 1 4.8 49 4* 7.0 50 3 6.2 60 4* 4.5 40 2 5.1 17-21 N MEAN S.D. 60 9 8.1 50 11 6.7 49 11 10.6 50 7 8.9 60 8 6.6 40 7 6.9 21-25 N MEAN S.D. 60 15 9.2 50 13 9.9 49 10* 11.3 50 16 9.3 60 9* 9.0 40 11 5.5 25-29 N MEAN S.D. 60 12 7.8 50 12 9.8 49 15 11.9 50 14 9.9 60 9 8.0 40 9 7.6 29-33 N MEAN S.D. 60 16 10.8 50 15 13.1 49 18 13.8 49 13 10.0 60 12 9.7 40 12 9.6 33-37 N MEAN S.D. 60 15 12.9 49 15 12.2 49 14 9.9 49 12 9.9 60 9* 8.2 40 9 10.5 196 Table 8 Summary of Body Weight Change Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 10 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 37-41 N MEAN S.D. 60 16 10.5 49 18 13.7 49 14 18.0 49 13 8.9 59 10* 9.3 40 8* 16.7 41-45 N MEAN S.D. 60 19 9.8 48 18 13.0 49 11 33.2 49 17 12.4 59 11* 7.5 39 14* 10.1 45-49 N MEAN S.D. 60 13 16.3 48 11 9.0 47 13 8.8 49 13 11.6 59 12 9.3 39 10 8.9 49-53 N MEAN S.D. 60 5 20.8 47 11* 10.6 47 13* 11.1 48 14* 10.4 59 7 10.5 39 10 7.3 53-57 N MEAN S.D. 50 18 13.4 47 16 17.4 47 19 17.8 48 21 11.3 48 14 13.2 39 27* 12.9 57-61 N MEAN S.D. 48 17 10.5 47 12 14.8 47 16 18.6 48 15 12.4 47 9* 13.4 39 26* 11.0 61-65 N MEAN S.D. 47 8 9.6 46 9 25.2 46 4 26.2 48 -1 21.3 46 5 19.5 39 14 19.3 197 Table 8 Covance 6329-183 3M T-6295 Summary of Body Weight Change Data (g) PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 65-69 N MEAN S.D. 47 14 12.8 43 14 19.0 42 13 23.7 47 15 19.9 44 14 9.6 38 24* 9.0 69-73 N MEAN S.D. 45 9 20.2 39 10 14.3 39 7 30.1 45 0 27.8 44 2 27.0 37 20* 10.0 73-77 N MEAN S.D. 44 3 23.1 39 11 14.5 37 1 29.6 44 -5 35.7 42 2 22.7 35 10 15.3 77-81 N MEAN S.D. 41 13 41.8 39 7 33.6 35 -10 37.5 39 2 25.8 40 5 21.2 35 4 24.7 81-85 N MEAN S.D. 37 3 19.0 37 3 31.3 26 -7 31.3 37 3 23.4 40 6 16.6 32 8 33.6 85-89 N MEAN S.D. 32 3 31.4 34 8 40.5 23 -8 39.0 37 3 27.6 38 4 17.1 29 9 22.5 89-93 N MEAN S.D. 31 -12 41.6 29 -12 35.3 16 -1 30.5 33 5 27.5 33 5 23.5 23 14* 19.4 198 Table 8 Covance 6329-183 3M T-6295 Summary of Body Weight Change Data (g) PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 93-97 N MEAN S.D. 26 -1 21.1 25 -14 45.6 14 0 26.6 25 2 38.8 30 10 23.9 22 4 37.5 97-101 N MEAN S.D. 26 4 30.5 19 -23 48.2 12 -6 20.9 19 -7 19.8 27 0 22.4 21 -15 47.9 101-105 N 24 15 0 17 26 19 MEAN -16 -26 -11 -4 -5 S.D. 27.6 57.1 44.1 45.3 39.9 1-15 N MEAN S.D. 60 149 35.2 50 151 30.8 49 140 20.5 50 144 28.2 60 114* 18.6 40 114* 17.8 15-29 N MEAN S.D. 50 100 56.5 47 107 42.7 47 111 48.4 48 102 30.7 48 74 30.8 39 92 36.0 29-57 N MEAN S.D. 60 38 16.2 50 43 20.3 49 44 18.6 50 44 20.2 60 33* 13.7 40 32 16.5 57-105 N 24 15 0 MEAN 84 81 S.D. 62.7 102.9 17 98 66.9 26 83 85.2 19 162* 117.8 1-105 N 24 15 0 17 26 19 MEAN 363 372 366 295 386 S.D. 103.9 114.7 82.4 100.8 133.9 199 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 1 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 69 MEAN 167 S.D. 13.3 56 165 11.5 60 165 14.1 59 164 13.2 69 158* 11.6 39 166 14.2 2 N 62 MEAN 177 S.D. 13.9 54 173 12.9 58 173 13.9 54 172 13.4 69 167* 13.8 37 172 17.2 3 N 68 MEAN 184 S.D. 12.9 52 183 13.0 56 181 14.1 57 182 13.4 70 178 15.2 38 181 16.0 4 N 60 MEAN 191 S.D. 16.5 47 188 13.7 49 184 19.3 48 177* 15.0 62 176* 14.7 38 184 17.4 5 N 62 MEAN 191 S.D. 16.1 50 193 16.1 50 186 16.7 49 185 16.3 64 183* 15.6 40 187 18.7 6 N 56 MEAN 192 S.D. 15.1 43 191 17.3 46 187 15.9 49 186 16.4 65 181* 15.9 38 185 17.6 7 N 59 MEAN 193 S.D. 15.6 42 195 18.3 47 183* 18.8 45 187 17.2 60 183* 15.4 37 184 19.9 200 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 2 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 54 MEAN 189 S.D. 19.2 45 193 17.9 47 188 17.7 43 185 15.3 60 178* 18.0 34 181 19.7 9 N 59 MEAN 190 S.D. 18.0 47 190 16.9 51 183 17.4 46 190 15.5 60 175* 17.0 32 182 17.8 10 N 59 MEAN 188 S.D. 18.0 48 189 17.6 46 188 17.2 48 183 15.6 59 177* 15.1 38 185 19.1 11 N 60 MEAN 188 S.D. 16.8 46 189 18.3 48 183 17.7 45 185 15.7 59 179* 17.8 38 182 18.9 12 N 57 MEAN 189 S.D. 20.1 51 189 18.5 52 184 20.5 49 185 16.7 61 180 21.1 39 178* 18.6 13 N 60 MEAN 185 S.D. 19.2 52 187 17.4 54 187 16.8 52 185 18.8 63 180 19.6 37 178 21.0 14 N 59 MEAN 184 S.D. 17.4 49 186 19.0 48 188 18.9 45 182 17.0 57 178 16.0 38 184 20.8 201 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 3 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 58 MEAN 190 S.D. 16.7 47 188 18.8 47 191 16.0 45 187 14.7 56 182 17.1 40 185 18.7 16 N 57 MEAN 190 S.D. 16.5 49 191 17.3 48 192 14.0 49 191 19.5 59 183 17.6 38 183 18.6 20 N 58 MEAN 192 S.D. 19.7 46 195 17.9 49 194 16.1 47 188 20.9 57 184 18.3 38 184 21.3 24 N 58 MEAN 184 S.D. 20.1 48 188 20.1 49 192 18.6 50 184 20.2 60 178 20.3 39 178 21.5 28 N 56 MEAN 183 S.D. 20.4 46 185 15.6 48 185 17.5 46 181 16.3 59 180 18.7 36 180 17.8 32 N 58 MEAN 177 S.D. 16.8 45 184 14.4 48 184 15.3 47 182 17.4 59 182 19.3 35 179 18.7 36 N 59 MEAN 183 S.D. 17.9 48 181 28.3 50 181 16.6 50 183 19.9 58 183 21.1 39 181 23.0 202 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 4 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N 58 MEAN 184 S.D. 19.1 46 184 19.1 48 186 16.2 48 187 20.3 58 187 22.5 39 182 22.7 44 N 58 MEAN 182 S.D. 24.9 48 188 21.0 49 185 14.7 47 186 20.0 55 184 20.7 39 188 23.9 48 N 57 MEAN 184 S.D. 20.2 48 191 18.5 48 186 18.8 47 185 18.3 55 184 20.8 37 185 18.3 52 N 54 MEAN 176 S.D. 27.6 46 185 17.8 48 186 17.3 44 181 27.5 54 178 22.4 37 179 20.9 56 N 45 MEAN 189 S.D. 27.2 43 196 18.8 44 195 16.5 44 193 20.4 46 190 32.0 35 190 22.1 60 N 45 MEAN 185 S.D. 39.2 46 194 29.6 45 197 20.3 44 198 28.9 44 200 25.1 37 194 32.0 64 N 44 MEAN 190 S.D. 29.6 42 192 15.6 44 193 20.8 43 189 22.2 42 197 23.2 35 174 46.6 203 Table 9 Covance 6329-183 3M T-6295 Summary of Food Consumption Data (g) PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N 43 MEAN 195 S.D. 25.8 43 187 41.2 46 194 33.0 42 201 37.8 43 199 26.7 29 186 38.9 72 N 40 MEAN 196 S.D. 25.4 43 180 43.4 45 192 30.1 43 197 24.5 43 193 43.7 31 198 32.1 76 N 38 MEAN 176 S.D. 42.9 41 170 47.5 43 176 52.1 38 193 26.4 38 190 33.7 30 188 39.1 80 N 31 MEAN 183 S.D. 45.0 36 168 35.9 36 187 35.0 37 191 20.3 37 189 35.0 28 185 37.4 84 N 27 MEAN 188 S.D. 30.1 31 172 43.5 32 183 48.9 36 186 33.9 34 196 34.3 24 187 35.3 88 N 25 MEAN 184 S.D. 43.4 24 175 43.8 29 189 29.6 34 169 64.4 35 199 26.8 22 190 29.5 92 N 21 MEAN 186 S.D. 23.3 21 184 26.0 26 185 36.7 24 198 16.0 34 185 42.8 18 180 42.6 204 Table 9 Covance 6329-183 3M T-6295 Summary of Food Consumption Data (g) PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------------MALE--------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N 17 MEAN 177 S.D. 51.1 19 167 35.7 21 186 47.0 25 194 20.1 30 183 46.0 15 158 48.7 100 N MEAN S.D. 14 175 53.4 14 166 46.8 17 197 34.1 24 188 29.7 27 188 37.0 11 170 43.4 104 N MEAN S.D. 10 167 30.1 10 158 59.5 14 185 45.0 23 169 53.6 22 173 50.1 10 155 53.1 205 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 7 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 55 MEAN 129 S.D. 11.9 55 120* 12.0 48 123 14.3 55 130 13.5 62 124 11.2 35 125 12.4 2 N 62 MEAN 140 S.D. 16.9 48 133 16.5 51 131* 17.8 47 136 19.6 62 130* 13.8 34 126* 12.7 3 N 54 MEAN 144 S.D. 16.8 49 142 17.0 51 140 17.6 55 141 14.9 57 131* 14.7 33 129* 13.3 4 N 47 MEAN 140 S.D. 15.9 44 137 17.5 41 142 20.1 45 138 18.3 58 126* 12.5 33 128* 14.0 5 N 50 MEAN 146 S.D. 18.5 48 146 17.6 43 144 14.2 45 144 19.3 55 135* 14.0 33 130* 11.4 6 N 45 MEAN 138 S.D. 18.6 40 142 23.3 35 137 20.3 42 134 17.3 55 124* 11.4 31 124* 13.1 7 N 54 MEAN 138 S.D. 17.0 49 142 17.1 45 133 12.2 46 136 16.3 53 126* 10.7 36 123* 12.0 206 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 8 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 46 MEAN 138 S.D. 18.5 38 138 15.9 43 136 11.3 42 135 15.5 54 123* 14.7 31 121* 12.6 9 N 55 MEAN 140 S.D. 18.8 48 135 15.2 41 132 17.0 46 134 17.3 56 122* 12.6 36 125* 17.5 10 N 53 MEAN 141 S.D. 18.2 45 135 16.0 39 134 11.0 45 135 13.8 53 124* 10.0 34 125* 14.4 11 N 56 MEAN 138 S.D. 21.7 52 134 15.5 46 133 13.4 48 134 16.0 55 122* 10.2 38 125* 14.8 12 N 52 MEAN 138 S.D. 20.1 43 133 15.7 39 137 17.6 48 136 21.5 58 127* 18.8 35 123* 11.7 13 N 56 MEAN 133 S.D. 17.7 46 135 17.7 47 131 13.1 49 131 20.1 59 122* 18.4 35 125 16.8 14 N 53 MEAN 131 S.D. 20.4 40 129 18.0 37 133 13.4 40 125 11.3 54 119* 11.3 32 122* 8.5 207 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 9 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N 53 MEAN 133 S.D. 17.1 49 134 15.9 43 135 14.9 45 133 16.2 57 124* 13.6 39 125 17.7 16 N 54 MEAN 133 S.D. 19.4 44 135 18.1 41 136 15.3 45 133 15.6 56 124* 12.2 34 124 15.0 20 N 50 MEAN 139 S.D. 19.0 46 134 16.0 42 136 16.2 44 138 18.8 49 128* 12.8 28 131 14.2 24 N 53 MEAN 134 S.D. 18.9 44 132 18.7 44 136 17.6 47 132 20.0 54 123* 14.0 32 122* 13.3 28 N 56 MEAN 132 S.D. 15.7 48 133 24.7 45 141 20.5 48 132 18.7 56 122* 20.4 40 121* 18.9 32 N 58 MEAN 136 S.D. 18.4 47 134 26.5 45 139 15.5 45 134 17.9 58 124* 17.4 40 127 17.7 36 N 55 MEAN 135 S.D. 17.9 49 135 20.9 46 137 15.3 47 133 17.8 58 124* 16.1 39 123* 14.9 208 Table 9 Summary of Food Consumption Data (g) Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: 10 PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N 56 MEAN 139 S.D. 20.3 44 139 15.6 48 142 20.6 47 138 17.6 56 135 20.7 38 130 14.7 44 N 56 MEAN 137 S.D. 17.8 45 134 19.3 45 137 18.6 47 134 20.7 53 126* 17.1 38 128 15.9 48 N 58 MEAN 134 S.D. 23.6 48 133 19.5 47 142 16.6 47 141 17.8 57 133 20.0 38 130 13.3 52 N 57 MEAN 133 S.D. 24.0 45 134 20.5 46 141 19.8 45 139 22.2 57 133 21.9 38 133 14.0 56 N 47 MEAN 147 S.D. 19.4 47 143 25.6 46 145 20.5 47 146 21.3 47 133* 22.4 35 142 18.5 60 N 47 MEAN 146 S.D. 19.1 47 137 23.2 46 146 28.2 48 142 22.3 44 131* 19.2 37 145 16.3 64 N 46 MEAN 146 S.D. 19.4 44 145 22.5 44 139 34.3 44 141 23.3 40 143 39.1 37 144 22.5 209 Table 9 Covance 6329-183 3M T-6295 Summary of Food Consumption Data (g) PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N 47 MEAN 151 S.D. 20.2 43 150 25.5 39 149 28.6 46 149 23.1 42 143 21.4 37 158 15.9 72 N 44 MEAN 143 S.D. 25.4 38 146 21.7 35 146 25.9 42 141 29.3 41 133 32.4 35 152 16.1 76 N 44 MEAN 141 S.D. 29.4 39 152 22.0 35 146 29.9 42 133 41.4 40 136 27.2 35 150 18.9 80 N 41 MEAN 147 S.D. 26.0 37 148 34.9 33 128 46.9 38 144 29.3 39 147 26.2 35 145 25.8 84 N 37 MEAN 147 S.D. 26.0 35 150 31.7 26 141 34.0 36 148 26.5 37 147 27.1 31 152 28.7 88 N 30 MEAN 142 S.D. 25.6 34 144 38.4 22 132 40.7 36 144 24.3 35 145 21.4 26 149 27.5 92 N 26 MEAN 140 S.D. 29.1 26 139 35.0 15 148 38.5 31 136 32.4 32 138 26.5 21 156 18.4 210 Table 9 Covance 6329-183 3M T-6295 Summary of Food Consumption Data (g) PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: --------------------------------FEMALE-------------------------------- GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N 26 MEAN 143 S.D. 24.3 22 131 40.9 13 145 36.7 23 135 43.8 28 141 29.6 17 141 40.2 100 N MEAN S.D. 24 137 30.1 17 115 48.8 12 148 33.9 16 141 28.5 25 139 32.5 20 135 41.4 104 N 24 10 0 17 23 18 MEAN 128 118 145 133 141 S.D. 37.6 40.5 48.4 39.9 40.5 211 Table 10 Covance 6329-183 3M T-6295 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------MALE--------------------------- --------------------------FEMALE-------------------------- GROUP: 2 3 4 5 6 2 3 4 5 6 DOSE: 0.5 2 5 20 20 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 1 N 56 60 59 69 39 55 48 55 62 35 MEAN 0.057 0.226 0.570 2.205 2.336 0.052 0.213 0.559 2.149 2.160 S.D. 0.004 0.015 0.041 0.156 0.170 0.004 0.020 0.046 0.141 0.178 2 N 54 58 54 69 37 48 51 47 62 34 MEAN 0.047 0.189 0.471 1.846 1.907 0.050 0.200 0.506 1.965 1.929 S.D. 0.003 0.012 0.031 0.115 0.118 0.005 0.019 0.055 0.162 0.164 3 N 52 56 57 70 38 49 51 55 57 33 MEAN 0.042 0.169 0.420 1.675 1.701 0.048 0.194 0.481 1.805 1.792 S.D. 0.002 0.010 0.026 0.097 0.097 0.003 0.026 0.038 0.157 0.126 4 N 47 49 48 62 38 44 41 45 58 33 MEAN 0.038 0.151 0.364 1.477 1.531 0.043 0.180 0.440 1.646 1.656 S.D. 0.002 0.011 0.020 0.081 0.086 0.003 0.018 0.049 0.121 0.142 5 N 50 50 49 64 40 48 43 45 55 33 MEAN 0.036 0.139 0.346 1.394 1.408 0.043 0.170 0.431 1.669 1.613 S.D. 0.002 0.007 0.019 0.072 0.073 0.003 0.011 0.036 0.137 0.113 6 N 43 46 49 65 38 40 35 42 55 31 MEAN 0.033 0.132 0.325 1.287 1.307 0.040 0.157 0.390 1.502 1.488 S.D. 0.002 0.007 0.017 0.065 0.068 0.005 0.019 0.034 0.106 0.107 7 N 42 47 45 60 37 49 45 46 53 36 MEAN 0.032 0.122 0.311 1.241 1.233 0.038 0.148 0.382 1.501 1.446 S.D. 0.002 0.010 0.016 0.061 0.078 0.003 0.010 0.028 0.112 0.089 212 Table 10 Covance 6329-183 3M T-6295 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------MALE--------------------------- --------------------------FEMALE-------------------------- GROUP: 2 3 4 5 6 2 3 4 5 6 DOSE: 0.5 2 5 20 20 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 8 N 45 47 43 60 34 38 43 42 54 31 MEAN 0.030 0.121 0.293 1.161 1.178 0.036 0.148 0.368 1.438 1.392 S.D. 0.002 0.007 0.012 0.093 0.106 0.002 0.011 0.030 0.133 0.099 9 N 47 51 46 60 32 48 41 46 56 36 MEAN 0.029 0.113 0.288 1.108 1.135 0.035 0.143 0.355 1.407 1.422 S.D. 0.001 0.006 0.016 0.076 0.063 0.002 0.023 0.027 0.120 0.202 10 N MEAN S.D. 48 0.028 0.002 46 0.113 0.006 48 0.270 0.017 59 1.074 0.060 38 1.130 0.080 45 0.033 0.002 39 0.138 0.010 45 0.355 0.033 53 1.393 0.101 34 1.407 0.111 11 N MEAN S.D. 46 0.027 0.002 48 0.108 0.007 45 0.266 0.014 59 1.056 0.060 38 1.086 0.071 52 0.033 0.003 46 0.136 0.011 48 0.346 0.029 55 1.361 0.101 38 1.393 0.120 12 N MEAN S.D. 51 0.026 0.002 52 0.106 0.009 49 0.262 0.016 61 1.055 0.101 39 1.050 0.075 43 0.032 0.003 39 0.137 0.015 48 0.344 0.040 58 1.406 0.193 35 1.363 0.126 13 N MEAN S.D. 52 0.025 0.002 54 0.105 0.006 52 0.256 0.017 63 1.029 0.093 37 1.024 0.080 46 0.032 0.003 47 0.129 0.011 49 0.324 0.026 59 1.330 0.194 35 1.355 0.130 14 N MEAN S.D. 49 0.025 0.002 48 0.102 0.007 45 0.248 0.018 57 1.007 0.073 38 1.043 0.104 40 0.030 0.004 37 0.130 0.012 40 0.310 0.031 54 1.289 0.118 32 1.313 0.101 213 Table 10 Covance 6329-183 3M T-6295 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------MALE--------------------------- --------------------------FEMALE-------------------------- GROUP: 2 3 4 5 6 2 3 4 5 6 DOSE: 0.5 2 5 20 20 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 15 N MEAN S.D. 47 0.025 0.002 47 0.102 0.006 45 0.250 0.013 56 1.010 0.074 40 1.032 0.069 49 0.031 0.004 43 0.129 0.011 45 0.318 0.023 57 1.330 0.121 39 1.333 0.160 16 N MEAN S.D. 49 0.025 0.002 48 0.100 0.006 49 0.247 0.017 59 1.006 0.084 38 1.010 0.067 44 0.031 0.004 41 0.129 0.016 45 0.315 0.027 56 1.315 0.113 34 1.302 0.132 20 N MEAN S.D. 46 0.024 0.001 49 0.096 0.007 47 0.234 0.020 57 0.969 0.065 38 0.970 0.073 46 0.030 0.003 42 0.124 0.012 44 0.316 0.029 49 1.305 0.104 28 1.345 0.105 24 N MEAN S.D. 48 0.022 0.002 49 0.090 0.007 50 0.219 0.018 60 0.904 0.070 39 0.904 0.084 44 0.028 0.004 44 0.120 0.015 47 0.288 0.024 54 1.218 0.110 32 1.200 0.098 28 N MEAN S.D. 46 0.021 0.002 48 0.083 0.006 46 0.206 0.016 59 0.875 0.082 36 0.872 0.069 48 0.028 0.005 45 0.121 0.022 48 0.279 0.025 56 1.181 0.164 40 1.157 0.174 32 N MEAN S.D. 45 0.019 0.001 48 0.080 0.006 47 0.199 0.012 59 0.850 0.083 35 0.837 0.059 47 0.027 0.006 45 0.112 0.013 45 0.273 0.028 58 1.152 0.111 40 1.170 0.155 36 N MEAN S.D. 48 0.019 0.003 50 0.076 0.008 50 0.195 0.021 58 0.817 0.084 39 0.805 0.046 49 0.026 0.003 46 0.106 0.012 47 0.261 0.023 58 1.111 0.099 39 1.103 0.080 214 Table 10 Covance 6329-183 3M T-6295 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------MALE--------------------------- --------------------------FEMALE-------------------------- GROUP: 2 3 4 5 6 2 3 4 5 6 DOSE: 0.5 2 5 20 20 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 40 N MEAN S.D. 46 0.018 0.002 48 0.076 0.005 48 0.196 0.020 58 0.812 0.090 39 0.794 0.052 44 0.025 0.003 48 0.106 0.014 47 0.263 0.025 56 1.179 0.132 38 1.122 0.092 44 N MEAN S.D. 48 0.018 0.002 49 0.074 0.006 47 0.188 0.017 55 0.786 0.069 39 0.801 0.055 45 0.024 0.003 45 0.098 0.012 47 0.244 0.027 53 1.069 0.112 38 1.065 0.128 48 N MEAN S.D. 48 0.018 0.002 48 0.072 0.007 47 0.184 0.017 55 0.765 0.080 37 0.780 0.049 48 0.023 0.003 47 0.099 0.013 47 0.247 0.020 57 1.100 0.137 38 1.054 0.119 52 N MEAN S.D. 46 0.017 0.002 48 0.071 0.006 44 0.174 0.025 54 0.714 0.082 37 0.732 0.053 45 0.022 0.003 46 0.095 0.012 45 0.237 0.028 57 1.070 0.125 38 1.047 0.097 56 N MEAN S.D. 43 0.018 0.001 44 0.073 0.007 44 0.183 0.016 46 0.750 0.099 35 0.000 0.000 47 0.023 0.004 46 0.095 0.014 47 0.238 0.029 47 1.036 0.127 35 0.000 0.000 60 N MEAN S.D. 46 0.018 0.003 45 0.073 0.008 44 0.183 0.025 44 0.783 0.076 37 0.000 0.000 47 0.021 0.003 46 0.090 0.017 48 0.223 0.026 44 0.994 0.123 37 0.000 0.000 64 N MEAN S.D. 42 0.017 0.002 44 0.071 0.010 43 0.173 0.017 42 0.740 0.071 35 0.000 0.000 44 0.022 0.003 44 0.085 0.018 44 0.222 0.031 40 1.094 0.403 37 0.000 0.000 215 Table 10 Covance 6329-183 3M T-6295 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------MALE--------------------------- --------------------------FEMALE-------------------------- GROUP: 2 3 4 5 6 2 3 4 5 6 DOSE: 0.5 2 5 20 20 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 68 N MEAN S.D. 43 0.017 0.004 46 0.071 0.012 42 0.186 0.056 43 0.755 0.113 29 0.000 0.000 43 0.022 0.003 39 0.087 0.015 46 0.227 0.030 42 1.054 0.142 37 0.000 0.000 72 N MEAN S.D. 43 0.016 0.004 45 0.070 0.010 43 0.175 0.019 43 0.708 0.146 31 0.000 0.000 38 0.021 0.002 35 0.084 0.014 42 0.209 0.027 41 0.957 0.205 35 0.000 0.000 76 N MEAN S.D. 41 0.015 0.004 43 0.064 0.017 38 0.173 0.018 38 0.700 0.120 30 0.000 0.000 39 0.021 0.003 35 0.082 0.013 42 0.197 0.052 40 0.971 0.148 35 0.000 0.000 80 N MEAN S.D. 36 0.016 0.003 36 0.068 0.011 37 0.171 0.021 37 0.700 0.139 28 0.000 0.000 37 0.020 0.004 33 0.073 0.023 38 0.212 0.034 39 1.019 0.187 35 0.000 0.000 84 N MEAN S.D. 31 0.016 0.004 32 0.065 0.017 36 0.171 0.027 34 0.711 0.109 24 0.000 0.000 35 0.020 0.004 26 0.078 0.016 36 0.216 0.029 37 1.001 0.132 31 0.000 0.000 88 N MEAN S.D. 24 0.016 0.003 29 0.068 0.007 34 0.154 0.055 35 0.724 0.099 22 0.000 0.000 34 0.019 0.004 22 0.073 0.022 36 0.208 0.032 35 0.984 0.165 26 0.000 0.000 92 N MEAN S.D. 21 0.017 0.002 26 0.065 0.011 24 0.171 0.018 34 0.676 0.174 18 0.000 0.000 26 0.018 0.004 15 0.075 0.015 31 0.191 0.041 32 0.912 0.148 21 0.000 0.000 216 Table 10 Covance 6329-183 3M T-6295 Summary of Test Material Consumption Data (mg/kg/day) PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ SEX: ---------------------------MALE--------------------------- --------------------------FEMALE-------------------------- GROUP: 2 3 4 5 6 2 3 4 5 6 DOSE: 0.5 2 5 20 20 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------------------ 96 N MEAN S.D. 19 0.016 0.003 21 0.068 0.014 25 0.171 0.022 30 0.685 0.170 15 0.000 0.000 22 0.017 0.005 13 0.075 0.021 23 0.186 0.049 28 0.906 0.162 17 0.000 0.000 100 N MEAN S.D. 14 0.016 0.004 17 0.069 0.011 24 0.165 0.026 27 0.708 0.156 11 0.000 0.000 17 0.015 0.006 12 0.077 0.019 16 0.190 0.026 25 0.876 0.193 20 0.000 0.000 104 N 10 14 23 22 10 10 0 MEAN 0.015 0.068 0.153 0.643 0.000 0.017 S.D. 0.005 0.020 0.041 0.162 0.000 0.005 17 0.195 0.056 23 0.838 0.247 18 0.000 0.000 217 Table 11 Summary of Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.47 15.2 44.8 60.0 20.3 33.9 1048 S.D. .459 .81 2.35 1.56 .73 .59 107.7 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 7.22 .414 10 14.7 .77 10 43.4 2.43 10 60.2 1.69 10 20.4 .67 10 33.9 .36 10 1082 111.4 10 2.0 MEAN S.D. N 7.54 .599 10 15.3 1.09 10 45.1 3.39 10 59.9 1.65 10 20.4 .85 10 34.0 .61 10 1028 68.7 10 5.0 MEAN S.D. N 7.43 .622 10 15.4 1.04 10 45.0 3.32 10 60.7 1.97 10 20.7 .68 10 34.1 .41 10 1013 118.6 10 20.0 MEAN S.D. N 7.30 .234 10 15.0 .44 10 44.2 1.56 10 60.6 1.40 10 20.6 .45 10 33.9 .37 10 1051 118.1 10 Covance 6329-183 3M T-6295 218 Table 11 Summary of Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.6 .7 7.2 .6 .1 .0 9 83 7 1 0 S.D. 2.03 .30 1.92 .16 .06 .00 3.5 5.3 2.2 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 9.3 1.2 7.4 .6 .1 .0 12 82 6 1 0 S.D. 2.54 1.32 1.29 .31 .06 .00 7.6 9.1 2.5 .3 .0 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 9.3 .7 8.0 .6 .1 .0 7 86 6 1 0 S.D. 2.30 .23 1.94 .26 .06 .00 1.4 3.4 2.3 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 7.2 .6 6.1 .4 .1 .0 8 85 6 2 0 S.D. 1.15 .21 .99 .24 .15 .00 2.3 6.5 2.9 2.0 .0 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 8.4 .8 6.8 .6 .1 .0 10 83 6 1 0 S.D. 2.87 .46 2.19 .38 .07 .00 2.8 4.8 2.4 .4 .0 N 10 10 10 10 10 10 10 10 10 10 10 219 Table 11 Summary of Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.28 15.2 45.1 62.0 20.8 33.7 1103 S.D. .276 .45 1.20 1.80 .63 .41 109.6 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 7.40 .367 10 15.3 .62 10 45.7 1.46 10 61.8 1.36 10 20.7 .58 10 33.5 .57 10 1126 181.3 10 2.0 MEAN S.D. N 7.49 .367 10 15.1 .70 10 45.2 1.64 10 60.4 1.62 10 20.2 .64 10 33.5 .55 10 1069 128.2 10 5.0 MEAN S.D. N 7.40 .396 10 15.4 .42 10 45.8 1.00 10 62.0 2.25 10 20.8 .79 10 33.6 .52 10 1104 116.6 10 20.0 MEAN S.D. N 7.38 .238 10 15.3 .27 10 45.6 1.07 10 61.9 1.21 10 20.8 .64 10 33.6 .53 10 1108 88.4 10 Covance 6329-183 3M T-6295 220 Table 11 Summary of Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.4 .7 6.3 .3 .1 .0 9 84 5 2 0 S.D. 2.27 .23 2.12 .20 .06 .00 3.5 4.0 2.2 1.4 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 6.5 .7 5.5 .3 .0 .0 10 84 4 1 0 S.D. 1.01 .78 1.01 .13 .05 .00 10.8 10.4 1.7 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 5.0 .4 4.3 .2 .0 .0 9 84 5 1 0 S.D. 1.80 .15 1.83 .11 .05 .00 5.1 7.9 3.5 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 6.9 .5 6.0 .2 .1 .0 8 87 4 1 0 S.D. 2.88 .23 2.62 .11 .04 .00 2.1 3.7 1.7 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 6.2 .5 5.4 .2 .1 .0 8 86 4 1 0 S.D. 1.80 .17 1.72 .14 .04 .00 2.7 4.6 2.1 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 221 Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.55 15.6 45.9 53.7 18.3 34.1 1142 S.D. .446 .69 1.83 1.52 .56 .55 164.6 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 8.77 .441 10 15.8 .56 10 46.7 1.45 10 53.3 1.68 10 18.0 .48 10 33.9 .53 10 1092 104.7 10 2.0 MEAN S.D. N 8.68 .456 10 15.7 .60 10 46.5 1.65 10 53.6 2.19 10 18.1 .60 10 33.7 .47 10 1168 189.6 10 5.0 MEAN S.D. N 8.57 .478 10 15.2 .63 10 45.2 1.61 10 52.8 1.64 10 17.8 .48 10 33.7 .49 10 1078 155.6 10 20.0 MEAN S.D. N 8.23 .216 10 15.1 .51 10 44.6 1.30 10 54.2 1.42 10 18.4 .51 10 33.9 .38 10 1139 124.3 10 Covance 6329-183 3M T-6295 222 Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.2 1.1 6.1 .8 .2 .0 14 74 10 2 0 S.D. 1.95 .37 1.46 .27 .07 .03 3.1 3.2 2.1 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 8.5 1.3 6.2 .8 .2 .0 15 73 10 2 0 S.D. 1.20 .28 1.04 .19 .10 .00 3.6 4.5 1.6 1.1 .0 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 7.8 1.2 5.7 .8 .2 .0 15 73 10 2 0 S.D. 1.64 .33 1.20 .29 .07 .03 1.8 3.8 2.8 1.1 .3 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 7.8 1.2 5.7 .7 .2 .0 16 73 9 2 0 S.D. 2.08 .37 1.97 .20 .05 .00 4.5 6.1 2.5 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 10.5 1.6 * 7.8 .9 .2 .0 16 73 8 2 0 S.D. 3.65 .35 3.43 .39 .08 .03 5.4 7.3 4.2 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 223 Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.00 15.3 45.2 56.6 19.1 33.8 1061 S.D. .535 .87 2.58 1.18 .25 .57 68.5 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 8.22 .377 10 15.5 .59 10 46.0 1.80 10 56.0 1.93 10 18.9 .74 10 33.7 .91 10 1143 67.8 10 2.0 MEAN S.D. N 8.06 .253 10 15.2 .58 10 45.2 1.76 10 56.1 2.37 10 18.8 .72 10 33.6 .57 10 1070 93.4 10 5.0 MEAN S.D. N 7.97 .453 10 15.2 .70 10 45.0 2.17 10 56.5 1.32 10 19.0 .41 10 33.7 .65 10 1082 64.7 10 20.0 MEAN S.D. N 7.87 .247 10 15.1 .67 10 44.2 1.82 10 56.2 1.64 10 19.2 .59 10 34.1 1.08 10 1144 122.9 10 Covance 6329-183 3M T-6295 224 Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN X103/.L BASO X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 4.9 1.0 3.4 .4 .1 .0 20 69 8 2 0 S.D. 1.51 .53 1.14 .17 .07 .00 8.8 9.7 2.9 .9 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 6.4 1.0 4.8 .5 .1 .0 16 73 9 2 0 S.D. 2.64 .45 2.18 .25 .03 .00 5.1 9.1 4.0 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 4.9 .7 3.9 .3 .1 .0 14 77 6 2 0 S.D. 1.65 .17 1.50 .08 .03 .00 4.4 5.1 1.2 .9 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 5.8 .9 4.4 .4 .1 .0 15 75 8 2 0 S.D. 1.96 .55 1.54 .32 .05 .03 7.1 7.7 3.9 .8 .4 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 4.8 1.0 3.4 .3 .1 .0 19 72 7 2 0 S.D. 1.68 .64 1.12 .17 .06 .00 6.3 6.7 2.5 1.3 .3 N 10 10 10 10 10 10 10 10 10 10 10 225 Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.78 15.7 46.1 52.6 17.9 34.0 1235 S.D. .471 .58 1.96 1.60 .56 .34 171.4 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 8.84 .629 10 15.5 .84 10 46.0 2.34 10 52.1 1.63 10 17.6 .60 10 33.7 .31 10 1270 138.4 10 2.0 MEAN S.D. N 8.82 .301 10 15.5 .54 10 45.6 1.62 10 51.8 1.01 10 17.5 .45 10 33.9 .38 10 1237 127.7 10 5.0 MEAN S.D. N 9.04 .439 10 15.8 .60 10 46.6 1.84 10 51.6 1.40 10 17.4 .58 10 33.8 .36 10 1217 116.4 10 20.0 MEAN S.D. N 8.67 .252 10 15.1 .62 10 44.7 1.22 10 51.6 2.06 10 17.4 .83 10 33.8 .57 10 1287 162.9 10 Covance 6329-183 3M T-6295 226 Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.5 1.3 5.0 1.0 .2 .0 18 67 13 2 1 S.D. 1.43 .29 1.14 .23 .08 .05 4.1 3.9 2.1 1.0 .5 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 8.2 1.5 5.6 .9 .2 .0 19 67 11 2 1 S.D. 1.42 .31 1.40 .19 .03 .05 5.6 6.2 2.3 .7 .5 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 8.7 1.7 5.7 1.0 .2 .0 19 66 12 2 0 S.D. 1.53 .52 1.28 .26 .07 .04 6.1 7.8 2.2 .6 .5 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 8.6 1.6 5.7 1.2 .2 .0 19 65 14 2 1 S.D. 1.88 .31 1.74 .32 .05 .05 4.5 6.7 3.0 .4 .5 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 8.7 1.6 5.9 1.0 .1 .0 18 68 12 2 0 S.D. 1.88 .42 1.41 .28 .05 .04 3.2 4.9 2.6 .7 .5 N 10 10 10 10 10 10 10 10 10 10 10 227 Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.56 14.6 43.5 57.6 19.2 33.4 1051 S.D. .563 .97 3.16 1.06 .33 .63 121.7 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 7.93 .639 10 14.9 .52 10 44.8 1.92 10 56.8 2.94 10 18.9 1.18 10 33.3 .35 10 1057 127.0 10 2.0 MEAN S.D. N 8.00 .312 10 15.1 .66 10 45.2 1.94 10 56.5 1.44 10 18.9 .53 10 33.4 .32 10 1074 75.0 10 5.0 MEAN S.D. N 8.12 .353 10 15.3 * .61 10 46.1 * 1.61 10 56.8 1.20 10 18.9 .57 10 33.3 .44 10 1095 131.4 10 20.0 MEAN S.D. N 7.73 .398 10 14.5 .53 10 43.3 1.70 10 56.1 1.87 10 18.8 .66 10 33.5 .39 10 1062 153.7 10 Covance 6329-183 3M T-6295 228 Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 5.5 .9 4.0 .6 .1 .0 16 72 10 2 0 S.D. .77 .54 .63 .26 .10 .03 9.1 9.9 3.3 1.3 .4 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 6.7 1.1 5.0 .5 .1 .0 14 77 7 2 0 S.D. 4.82 1.22 3.24 .33 .05 .03 5.3 5.6 3.2 .6 .3 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 4.8 .7 3.6 .4 .1 .0 15 74 8 2 0 S.D. 1.17 .21 .97 .18 .03 .00 3.1 6.0 3.4 1.1 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 6.1 .8 4.7 .5 .1 .0 14 76 8 2 0 S.D. 1.95 .23 1.74 .24 .05 .00 4.8 6.5 3.9 .6 .4 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 5.9 .9 4.4 .4 .1 .0 16 75 7 2 0 S.D. 1.61 .55 1.41 .18 .04 .00 8.4 10.7 3.2 1.0 .5 N 10 10 10 10 10 10 10 10 10 10 10 229 Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 8.33 15.5 43.5 52.3 18.6 35.6 1112 S.D. .286 .70 2.02 1.70 .59 .38 155.9 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 8.33 .266 10 15.2 .21 10 43.4 .77 10 52.1 1.23 10 18.3 .57 10 35.1 .49 10 1059 172.9 10 2.0 MEAN S.D. N 8.11 .288 10 14.9 .64 10 42.5 1.63 10 52.4 1.79 10 18.4 .75 10 35.1 .36 10 931 * 108.6 10 5.0 MEAN S.D. N 8.43 .536 10 15.2 .45 10 43.7 1.56 10 52.0 2.41 10 18.0 .94 10 34.7 * .58 10 1011 111.9 10 20.0 MEAN S.D. N 8.28 .360 9 14.9 .71 9 41.7 1.74 9 50.4 1.58 9 17.9 .47 9 35.6 .49 9 1241 124.3 9 Covance 6329-183 3M T-6295 230 Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.1 2.4 3.6 .9 .1 .0 34 51 13 2 0 S.D. 1.33 .96 .96 .26 .05 .00 11.9 10.6 3.2 .6 .4 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 7.8 1.7 4.9 * 1.1 .1 .0 22 * 63 * 14 2 0 S.D. 1.59 .31 1.10 .32 .06 .03 3.0 4.0 2.5 .7 .4 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 8.6 2.1 5.4 * 1.0 .1 .0 22 * 64 * 11 1 0 S.D. 2.97 1.64 1.20 .65 .04 .03 7.6 10.2 4.5 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 8.5 2.0 5.4 * .9 .1 .0 24 63 * 11 2 0 S.D. 1.63 .74 1.28 .33 .04 .00 6.2 7.9 2.6 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 7.3 2.3 4.0 .8 .1 .0 30 56 12 2 0 S.D. 1.78 1.58 1.29 .42 .03 .03 12.3 15.1 5.0 .5 .3 N 9 9 9 9 9 9 9 9 9 9 9 231 Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/.L G/DL % FL PG % X103/.L ------ -------- -------- -------- -------- -------- -------- -------- 0 MEAN 7.39 14.5 40.5 54.9 19.6 35.7 1009 S.D. .485 .76 1.84 1.64 .60 .31 80.9 N 10 10 10 10 10 10 10 0.5 MEAN S.D. N 7.26 .695 10 14.2 1.04 10 41.0 3.11 10 56.6 2.40 10 19.7 .94 10 34.7 * .42 10 908 99.0 10 2.0 MEAN S.D. N 7.31 .566 10 14.3 .61 10 41.1 1.91 10 56.3 2.32 10 19.7 .89 10 34.9 * .45 10 851 * 101.9 10 5.0 MEAN S.D. N 7.32 .479 10 14.2 .56 10 41.1 1.73 10 56.2 1.96 10 19.5 .83 10 34.7 * .40 10 837 * 85.9 10 20.0 MEAN S.D. N 7.05 .881 10 13.5 1.66 10 38.1 4.56 10 54.0 1.25 10 19.2 .46 10 35.5 .29 10 1023 113.7 10 Covance 6329-183 3M T-6295 232 Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 DOSE WBC ppm T-6295 X103/.L N-SEG X103/.L LYMPH X103/.L MONO X103/.L EOSIN BASO X103/.L X103/.L N-SEG% LYMPH% MONO% EOSIN% BASO% ------ -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 4.2 1.6 1.9 .5 .1 .0 37 49 12 2 0 S.D. 1.72 1.16 .57 .16 .00 .00 10.5 8.8 3.2 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 4.7 1.0 3.1 * .5 .1 .0 22 * 64 * 12 2 0 S.D. 1.53 .28 1.27 .20 .03 .00 4.7 7.2 4.6 .8 .3 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 5.2 1.5 3.1 * .5 .1 .0 28 59 10 3 0 S.D. 1.28 .54 .94 .16 .05 .00 7.4 8.6 2.5 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 4.2 1.2 2.5 .5 .1 .0 27 60 11 2 0 S.D. 1.27 .58 .70 .19 .04 .00 8.2 8.2 3.9 .8 .3 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 5.4 2.1 2.6 .5 .1 .0 34 53 10 2 0 S.D. 2.33 1.94 .90 .27 .04 .00 17.1 17.6 2.5 1.0 .4 N 10 10 10 10 10 10 10 10 10 10 10 233 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 97 12 .6 6.2 4.4 1.8 .1 49 S.D. 11.4 1.2 .18 .43 .26 .32 .09 6.6 N 10 10 10 10 10 10 10 10 0.5 MEAN 97 12 .6 6.4 4.6 1.8 .1 49 S.D. 5.2 1.8 .06 .33 .29 .31 .09 6.5 N 10 10 10 10 10 10 10 10 2.0 MEAN 91 12 .6 6.0 4.5 1.6 .1 44 S.D. 10.8 1.3 .14 .33 .29 .16 .08 8.6 N 10 10 10 10 10 10 10 10 5.0 MEAN 94 12 .6 6.3 4.6 1.7 .0 48 S.D. 8.9 2.1 .10 .29 .19 .15 .05 15.3 N 10 10 10 10 10 10 10 10 20.0 MEAN 84 * 13 .6 6.3 4.6 1.7 .1 45 S.D. 5.0 1.5 .12 .23 .18 .13 .09 13.7 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 234 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 122 26.4 10 31 3.4 10 1 1.3 10 10.7 .41 10 10.0 .54 10 148 1.6 10 6.3 .69 10 104 1.5 10 0.5 MEAN S.D. N 146 28.9 10 32 4.2 10 0 .7 10 10.7 .31 10 9.5 .62 10 147 1.3 10 6.3 .42 10 104 1.1 10 2.0 MEAN S.D. N 104 22.6 10 28 5.2 10 1 1.6 10 10.6 .41 10 10.1 .39 10 148 1.4 10 6.2 .71 10 105 1.1 10 5.0 MEAN S.D. N 114 17.2 10 30 3.8 10 0 .7 10 10.9 .28 10 9.8 .62 10 147 1.4 10 6.2 .62 10 103 2.2 10 20.0 MEAN S.D. N 131 19.9 10 33 3.0 10 1 1.3 10 10.6 .31 10 9.8 .59 10 148 1.4 10 6.1 .58 10 103 .9 10 Covance 6329-183 3M T-6295 235 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 114 14 .7 6.9 5.1 1.8 .1 72 S.D. 7.1 1.7 .10 .14 .11 .16 .10 11.5 N 10 10 10 10 10 10 10 10 0.5 MEAN 111 13 .8 6.8 5.0 1.8 .0 70 S.D. 7.0 1.8 .09 .16 .18 .23 .07 25.8 N 10 10 10 10 10 10 10 10 2.0 MEAN 113 14 .8 6.8 5.1 1.7 .0 61 S.D. 17.9 1.8 .13 .39 .32 .19 .07 17.7 N 10 10 10 10 10 10 10 10 5.0 MEAN 109 13 .7 7.1 5.3 1.8 .0 58 S.D. 11.0 1.2 .08 .35 .31 .17 .04 14.8 N 10 10 10 10 10 10 10 10 20.0 MEAN 107 14 .7 6.8 5.2 1.6 .0 54 S.D. 7.7 1.8 .10 .35 .19 .18 .10 10.7 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 236 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 123 28.2 10 32 4.8 10 0 .7 10 10.8 .34 10 8.5 .90 10 145 1.0 10 5.9 .43 10 104 1.3 10 0.5 MEAN S.D. N 120 37.2 10 31 5.6 10 1 1.4 10 10.6 .31 10 8.4 1.03 10 145 .8 10 6.1 .45 10 104 .9 10 2.0 MEAN S.D. N 101 12.2 10 29 5.6 10 2 2.0 10 10.7 .37 10 8.5 1.08 10 147 1.3 10 6.2 .51 10 106 1.2 10 5.0 MEAN S.D. N 112 23.9 10 32 5.1 10 2 1.3 10 10.7 .35 10 7.9 .83 10 147 2.2 10 6.1 .41 10 105 1.5 10 20.0 MEAN S.D. N 92 * 16.0 10 32 7.1 10 1 1.1 10 10.6 .24 10 8.2 1.47 10 146 1.8 10 6.0 .56 10 105 1.1 10 Covance 6329-183 3M T-6295 237 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 PCOAO IU/G ------ -------- 0 MEAN 9 S.D. 2.2 N 5 0.5 MEAN 9 S.D. 2.3 N 5 2.0 MEAN 7 S.D. 4.0 N 5 5.0 MEAN 8 S.D. .8 N 5 20.0 MEAN 6 S.D. 1.4 N 5 Covance 6329-183 3M T-6295 238 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 PCOAO IU/G ------ -------- 0 MEAN 5 S.D. 1.5 N 5 0.5 MEAN 6 S.D. 1.1 N 5 2.0 MEAN 3 S.D. 1.7 N 5 5.0 MEAN 2* S.D. 1.1 N 5 20.0 MEAN 4 S.D. 1.1 N 5 Covance 6329-183 3M T-6295 239 Table 16 Summary of Clinical Chemistry Data Males Week 14 (PCOAO) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 PCOAO IU/G ------ -------- 0 MEAN 5 S.D. 1.3 N 5 0.5 MEAN 5 S.D. 3.3 N 5 2.0 MEAN 5 S.D. 3.0 N 5 5.0 MEAN 2 S.D. 1.8 N 5 20.0 MEAN 5 S.D. 1.9 N 5 Covance 6329-183 3M T-6295 240 Table 16 Summary of Clinical Chemistry Data Females Week 14 (PCOAO) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 PCOAO IU/G ------ -------- 0 MEAN 2 S.D. 1.6 N 5 0.5 MEAN 3 S.D. 2.4 N 5 2.0 MEAN 1 S.D. .7 N 5 5.0 MEAN 2 S.D. 2.6 N 5 20.0 MEAN 5 S.D. 2.9 N 5 Covance 6329-183 3M T-6295 241 Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 102 13 .4 6.9 4.9 2.0 .2 63 S.D. 6.5 1.8 .14 .41 .26 .30 .18 13.4 N 10 10 10 10 10 10 10 10 0.5 MEAN 106 14 .6 7.0 5.0 2.0 .2 53 S.D. 11.2 1.6 .14 .34 .20 .26 .15 16.6 N 10 10 10 10 10 10 10 10 2.0 MEAN 91 13 .5 6.7 4.7 2.0 .1 51 S.D. 13.7 2.0 .10 .34 .16 .25 .12 14.5 N 10 10 10 10 10 10 10 10 5.0 MEAN 99 14 .6 7.0 4.9 2.1 .2 57 S.D. 8.9 1.4 .12 .46 .29 .20 .15 6.9 N 10 10 10 10 10 10 10 10 20.0 MEAN 95 16 * .6 6.9 4.9 2.0 .1 37 * S.D. 10.3 1.6 .16 .34 .24 .18 .13 12.9 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 242 Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 111 23.1 10 36 7.0 10 1 1.5 10 10.5 .28 10 7.5 .57 10 146 .7 10 6.2 .37 10 105 1.2 10 0.5 MEAN S.D. N 112 18.8 10 41 6.3 10 1 .7 10 10.5 .29 10 7.8 .64 10 147 2.6 10 6.5 .32 10 105 2.0 10 2.0 MEAN S.D. N 127 20.5 10 41 4.7 10 1 1.3 10 10.4 .20 10 7.8 .64 10 147 1.8 10 6.4 .46 10 105 2.2 10 5.0 MEAN S.D. N 129 15.4 10 44 13.6 10 1 1.6 10 10.5 .31 10 7.5 .57 10 147 2.8 10 6.1 .37 10 104 2.0 10 20.0 MEAN S.D. N 128 60.7 10 65 * 52.9 10 1 1.6 10 10.5 .23 10 7.8 .58 10 146 1.3 10 6.4 .52 10 104 1.4 10 Covance 6329-183 3M T-6295 243 Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 106 15 .6 7.6 5.6 2.1 .1 75 S.D. 12.0 1.7 .12 .34 .25 .22 .19 14.9 N 10 10 10 10 10 10 10 10 0.5 MEAN 106 15 .6 8.2 * 6.2 * 2.0 .2 88 S.D. 9.1 3.2 .13 .42 .44 .19 .14 27.1 N 10 10 10 10 10 10 10 10 2.0 MEAN 108 15 .8 * 7.9 5.9 2.0 .1 87 S.D. 5.9 2.3 .12 .30 .24 .22 .08 23.9 N 10 10 10 10 10 10 10 10 5.0 MEAN 95 * 15 .6 8.0 5.9 2.1 .2 70 S.D. 8.3 2.0 .14 .44 .43 .31 .14 13.4 N 10 10 10 10 10 10 10 10 20.0 MEAN 99 18 * .6 8.0 6.1 * 1.9 .2 66 S.D. 6.7 2.5 .05 .45 .33 .19 .14 14.0 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 244 Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 103 16.3 10 34 2.4 10 1 1.2 10 10.7 .45 10 6.3 .95 10 148 2.0 10 6.0 .43 10 107 .9 10 0.5 MEAN S.D. N 107 17.8 10 36 9.1 10 1 1.7 10 11.1 .49 10 6.4 1.30 10 148 2.1 10 6.4 .76 10 106 1.6 10 2.0 MEAN S.D. N 103 26.9 10 37 17.9 10 1 1.4 10 10.9 .43 10 6.5 .87 10 148 3.0 10 6.2 .71 10 105 1.4 10 5.0 MEAN S.D. N 103 16.5 10 34 4.7 10 2 1.9 10 10.7 .51 10 6.6 1.14 10 148 2.8 10 5.8 .72 10 107 1.9 10 20.0 MEAN S.D. N 90 20.7 10 39 18.2 10 1 1.5 10 10.8 .30 10 6.5 .77 10 148 2.6 10 6.2 .57 10 106 3.0 10 Covance 6329-183 3M T-6295 245 Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 115 12 .7 7.3 4.9 2.4 .2 89 S.D. 8.1 2.3 .10 .43 .28 .34 .12 24.7 N 10 10 10 10 10 10 10 10 0.5 MEAN 117 13 .8 7.1 4.7 2.4 .3 84 S.D. 14.7 1.7 .16 .48 .30 .33 .06 31.8 N 10 10 10 10 10 10 10 10 2.0 MEAN 114 13 .8 7.2 4.8 2.4 .4 74 S.D. 13.2 2.3 .08 .29 .23 .26 .16 15.5 N 10 10 10 10 10 10 10 10 5.0 MEAN 113 14 .7 7.4 4.9 2.5 .3 72 S.D. 11.7 2.5 .16 .45 .28 .24 .12 28.2 N 10 10 10 10 10 10 10 10 20.0 MEAN 111 17 * .8 7.5 5.1 2.4 .2 46 * S.D. 12.8 2.1 .15 .34 .25 .19 .10 11.6 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 246 Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 84 17.8 10 37 6.2 10 1 1.3 10 11.1 .36 10 6.7 .36 10 146 1.1 10 6.7 .23 10 104 2.0 10 0.5 MEAN S.D. N 81 13.0 10 36 6.1 10 2 1.8 10 11.1 .43 10 6.9 .59 10 147 1.8 10 7.0 .53 10 104 1.4 10 2.0 MEAN S.D. N 92 22.0 10 47 10.5 10 1 .6 10 11.1 .28 10 6.8 .30 10 147 1.9 10 6.7 .54 10 104 1.5 10 5.0 MEAN S.D. N 80 9.7 10 38 6.3 10 1 1.4 10 11.3 .57 10 7.1 .62 10 148 1.9 10 6.9 .46 10 104 1.4 10 20.0 MEAN S.D. N 103 53.6 10 65 48.7 10 1 1.9 10 11.2 .34 10 7.3 .92 10 147 2.5 10 6.9 .65 10 104 1.4 10 Covance 6329-183 3M T-6295 247 Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 104 14 .9 8.9 6.4 2.5 S.D. 7.3 1.3 .17 .37 .41 .20 N 10 10 10 10 10 10 .4 115 .14 26.0 10 10 0.5 MEAN 98 15 .9 8.4 6.2 2.3 .3 95 S.D. 6.3 2.1 .21 .66 .59 .29 .10 29.0 N 10 10 10 10 10 10 10 10 2.0 MEAN 99 15 1.0 8.4 6.0 2.4 .3 78 * S.D. 8.2 1.9 .16 .71 .65 .26 .13 16.3 N 10 10 10 10 10 10 10 10 5.0 MEAN 98 15 .9 8.3 5.9 2.3 .3 79 * S.D. 10.0 3.2 .14 .39 .28 .29 .15 10.9 N 10 10 10 10 10 10 10 10 20.0 MEAN 94 20 * .9 8.7 6.4 2.3 .4 80 * S.D. 6.8 2.8 .13 .55 .37 .31 .14 24.4 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 248 Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 119 71.9 10 64 49.4 10 2 2.0 10 11.7 .34 10 6.7 .77 10 148 2.1 10 5.8 .71 10 106 2.3 10 0.5 MEAN S.D. N 102 18.6 10 34 8.4 10 2 1.5 10 11.7 .67 10 6.5 .74 10 147 2.2 10 5.9 .69 10 106 1.9 10 2.0 MEAN S.D. N 143 116.7 10 77 130.6 10 2 2.0 10 11.2 * .38 10 6.4 .96 10 148 2.4 10 5.8 .57 10 107 1.8 10 5.0 MEAN S.D. N 117 39.6 10 47 25.8 10 4 1.7 10 11.2 .37 10 6.7 .75 10 148 1.9 10 6.0 .63 10 108 2.0 10 20.0 MEAN S.D. N 117 62.6 10 64 55.5 10 2 2.6 10 11.5 .44 10 7.3 1.20 10 148 2.9 10 6.0 .67 10 106 2.0 10 Covance 6329-183 3M T-6295 249 Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 129 10 .6 7.0 4.4 2.6 S.D. 13.2 1.4 .17 .46 .35 .33 N 10 10 10 10 10 10 .0 120 .07 44.6 10 10 0.5 MEAN 125 12 .7 7.4 4.8 2.6 S.D. 12.5 1.2 .09 .26 .26 .28 N 10 10 10 10 10 10 .2 129 .16 53.1 10 10 2.0 MEAN 121 12 * .7 7.3 4.7 2.6 .1 89 S.D. 13.8 1.5 .15 .38 .20 .32 .09 17.7 N 10 10 10 10 10 10 10 10 5.0 MEAN 121 14 * .7 7.6 * 4.8 2.7 .1 92 S.D. 8.0 1.3 .16 .46 .28 .32 .12 15.2 N 10 10 10 10 10 10 10 10 20.0 MEAN 110 * 13 * .7 7.0 4.6 2.4 .0 50 * S.D. 10.2 2.3 .09 .61 .55 .23 .03 17.8 N 9 9 9 9 9 9 9 9 Covance 6329-183 3M T-6295 250 Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 106 27.0 10 54 65.8 10 0 .0 10 10.5 .39 10 6.3 .42 10 147 1.6 10 5.9 .44 10 106 1.8 10 0.5 MEAN S.D. N 118 38.8 10 62 * 51.8 10 0 .5 10 11.4 * .41 10 7.1 * .34 10 148 2.5 10 6.5 .55 10 106 1.9 10 2.0 MEAN S.D. N 111 16.6 10 40 7.5 10 0 .8 10 11.0 * .43 10 7.2 * .59 10 149 2.4 10 6.6 .56 10 107 1.7 10 5.0 MEAN S.D. N 130 21.9 10 44 8.3 10 0 1.3 10 11.1 * .55 10 7.7 * .64 10 149 3.5 10 6.8 * .63 10 107 2.4 10 20.0 MEAN S.D. N 129 70.0 9 83 * 84.4 9 1 10.5 1.3 .41 9 9 7.0 * .61 9 148 3.5 9 6.2 .89 9 110 * 2.1 9 Covance 6329-183 3M T-6295 251 Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 111 11 .7 7.7 5.5 2.2 S.D. 10.1 2.5 .10 1.04 .81 .38 N 10 10 10 10 10 10 .0 116 .07 19.8 10 10 0.5 MEAN 102 13 .7 8.1 5.8 2.3 S.D. 8.4 2.3 .25 .44 .34 .19 N 10 10 10 10 10 10 .2 108 .14 26.1 10 10 2.0 MEAN S.D. N 96 * 13 .8 8.0 5.5 2.5 12.9 2.2 .15 .45 .44 .46 10 10 10 10 10 10 .1 101 .11 17.2 10 10 5.0 MEAN S.D. N 97 * 14 * .8 8.2 5.8 2.4 6.3 2.9 .19 .31 .32 .30 10 10 10 10 10 10 .1 110 .13 49.6 10 10 20.0 MEAN 98 * 16 * .8 7.9 5.7 2.2 .0 79 S.D. 9.8 2.8 .14 .76 .81 .39 .10 25.5 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 252 Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T-6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L ------ -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 119 61.0 10 50 30.2 10 0 .3 10 10.6 .54 10 5.8 .90 10 146 2.4 10 5.1 .47 10 106 2.6 10 0.5 MEAN S.D. N 115 15.3 10 43 7.1 10 0 .5 10 11.1 * .48 10 5.8 .83 10 147 3.6 10 5.8 1.04 10 106 3.7 10 2.0 MEAN S.D. N 121 21.4 10 54 14.5 10 1 1.3 10 10.8 .24 10 5.6 .58 10 147 2.1 10 5.1 .45 10 107 2.5 10 5.0 MEAN S.D. N 108 25.1 10 46 19.5 10 1 1.7 10 11.0 .29 10 5.5 .74 10 147 1.8 10 5.4 .65 10 108 3.2 10 20.0 MEAN S.D. N 93 17.5 10 46 17.3 10 0 .3 10 10.6 .47 10 5.9 .65 10 147 3.1 10 5.3 1.00 10 110 2.7 10 Covance 6329-183 3M T-6295 253 Table 20 Summary of Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 CHOL MG/DL TRIG MG/DL ------ -------- -------- 0 MEAN S.D. N 153 73.5 11 199 151.3 11 0.5 MEAN S.D. N 144 60.8 10 187 82.7 10 2.0 MEAN S.D. N 129 38.4 17 199 120.6 17 5.0 MEAN S.D. N 123 42.7 25 150 65.3 25 20.0 MEAN S.D. N 118 52.2 22 169 121.9 22 20.0 MEAN S.D. N 145 40.1 10 137 53.9 10 Covance 6329-183 3M T-6295 254 Table 20 Summary of Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 CHOL MG/DL TRIG MG/DL ------ -------- -------- 0 MEAN S.D. N 124 39.6 24 123 78.6 24 0.5 MEAN S.D. N 124 25.4 15 114 100.1 15 5.0 MEAN S.D. N 114 29.8 15 136 92.5 15 20.0 MEAN S.D. N 103 57.2 25 96 109.7 25 20.0 MEAN S.D. N 109 23.3 17 149 76.2 17 Covance 6329-183 3M T-6295 255 Table 21 Summary of Clinical Chemistry Data Females Day 719 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 CHOL MG/DL TRIG MG/DL ------ -------- -------- 2.0 MEAN S.D. N 124 41.1 9 169 70.4 9 Covance 6329-183 3M T-6295 256 Table 22 Summary of Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 21.4 1.010 S.D. 11.72 .0036 N 10 10 7.0 .16 10 0.5 MEAN S.D. N 22.6 14.76 10 1.014 .0080 10 7.0 .24 10 2.0 MEAN S.D. N 25.3 16.35 10 1.010 .0073 10 7.0 .33 10 5.0 MEAN S.D. N 31.8 14.52 10 1.009 .0055 10 7.2 .24 10 20.0 MEAN S.D. N 22.6 9.78 10 1.012 .0069 10 7.0 .00 10 Covance 6329-183 3M T-6295 257 Table 22 Summary of Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 10.9 1.019 S.D. 13.48 .0103 N 10 10 6.8 .26 10 0.5 MEAN S.D. N 15.2 16.13 10 1.015 .0106 10 6.8 .24 10 2.0 MEAN S.D. N 14.2 17.68 10 1.015 .0087 10 6.8 .26 10 5.0 MEAN S.D. N 13.2 7.12 10 1.014 .0060 10 6.8 .24 10 20.0 MEAN S.D. N 17.5 16.39 10 1.013 .0083 10 6.8 .26 10 Covance 6329-183 3M T-6295 258 Table 23 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 28.8 1.014 S.D. 16.45 .0099 N 10 10 7.2 .34 10 0.5 MEAN S.D. N 23.5 16.23 10 1.018 .0140 10 7.1 .39 10 2.0 MEAN S.D. N 20.8 17.37 10 1.018 .0099 10 7.0 .37 10 5.0 MEAN S.D. N 25.4 14.27 10 1.014 .0067 10 7.0 .33 10 20.0 MEAN S.D. N 18.5 14.64 10 1.019 .0073 10 7.0 .28 10 Covance 6329-183 3M T-6295 259 Table 23 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 17.5 1.020 S.D. 21.04 .0166 N 10 10 6.8 .26 10 0.5 MEAN S.D. N 16.8 17.86 10 1.016 .0090 10 7.0 .50 10 2.0 MEAN S.D. N 7.8 6.43 10 1.021 .0082 10 6.8 .35 10 5.0 MEAN S.D. N 25.7 14.20 10 1.009 .0050 10 6.8 .35 10 20.0 MEAN S.D. N 19.5 17.83 10 1.015 .0119 10 6.9 .32 10 Covance 6329-183 3M T-6295 260 Table 24 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 15.2 1.022 S.D. 7.12 .0105 N 10 10 6.9 .32 10 0.5 MEAN S.D. N 16.2 8.42 10 1.017 .0056 10 7.1 .21 10 2.0 MEAN S.D. N 16.2 6.79 10 1.019 .0053 10 7.0 .37 10 5.0 MEAN S.D. N 20.4 15.70 10 1.019 .0107 10 7.0 .24 10 20.0 MEAN S.D. N 17.9 16.42 10 1.029 .0231 10 6.8 .26 10 Covance 6329-183 3M T-6295 261 Table 24 Summary of Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 17.6 1.016 S.D. 17.05 .0142 N 10 10 6.6 .21 10 0.5 MEAN S.D. N 15.9 12.34 10 1.016 .0089 10 6.6 .34 10 2.0 MEAN S.D. N 15.6 12.72 10 1.016 .0092 10 6.8 .24 10 5.0 MEAN S.D. N 21.6 14.18 10 1.014 .0104 10 6.9 .32 10 20.0 MEAN S.D. N 13.7 11.58 10 1.022 .0170 10 6.6 .37 10 Covance 6329-183 3M T-6295 262 Table 25 Summary of Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN 12.5 1.025 S.D. 7.75 .0110 N 10 10 6.6 .32 10 0.5 MEAN S.D. N 18.0 13.20 10 1.026 .0123 10 6.7 .26 10 2.0 MEAN S.D. N 16.2 7.90 10 1.021 .0063 10 7.1 * .42 9 5.0 MEAN S.D. N 11.6 7.53 10 1.032 .0182 10 6.9 .39 10 20.0 MEAN S.D. N 11.0 4.55 9 1.028 .0074 9 6.9 .49 9 Covance 6329-183 3M T-6295 263 Table 25 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U VOL ML SP GR U PH ------ -------- -------- -------- 0 MEAN S.D. N 9.1 2.00 10 1.022 .0039 10 6.5 .33 10 0.5 MEAN S.D. N 7.7 4.07 10 1.025 .0070 10 6.6 .52 10 2.0 MEAN S.D. N 9.0 5.46 10 1.025 .0114 10 6.5 .33 10 5.0 MEAN S.D. N 8.3 12.54 10 1.030 .0105 10 6.6 .41 10 20.0 MEAN S.D. N 10.1 13.35 10 1.028 .0117 10 6.2 .26 10 Covance 6329-183 3M T-6295 264 Table 26 Summary of Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 12 3.5 10 65 20.2 10 .22 .092 9 1.18 .258 9 0.5 MEAN S.D. N 19 13.9 10 86 53.4 10 .29 .096 9 1.35 .191 9 2.0 MEAN S.D. N 13 9.5 10 65 42.5 10 .24 .114 7 1.18 .211 7 5.0 MEAN S.D. N 14 7.9 10 54 32.0 10 .40 .186 7 1.47 .289 7 20.0 MEAN S.D. N 17 16.4 10 68 39.1 10 .28 .086 10 1.23 .175 10 Covance 6329-183 3M T-6295 265 Table 26 Summary of Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 22 10.6 10 103 59.9 10 .13 .049 9 .56 .198 9 0.5 MEAN S.D. N 23 18.0 10 86 74.1 10 .15 .071 8 .59 .152 8 2.0 MEAN S.D. N 28 16.7 10 80 43.9 10 .20 .069 8 .57 .198 8 5.0 MEAN S.D. N 22 12.2 10 70 34.1 10 .24 * .076 10 .75 .222 10 20.0 MEAN S.D. N 20 12.3 10 67 40.2 10 .18 .048 8 .66 .130 8 Covance 6329-183 3M T-6295 266 Table 27 Summary of Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 20 17.1 10 60 50.1 10 .43 .255 7 1.17 .294 7 0.5 MEAN S.D. N 25 16.5 10 87 80.7 10 .38 .165 8 1.13 .314 8 2.0 MEAN S.D. N 23 12.5 10 82 56.0 10 .33 .180 8 1.01 .372 8 5.0 MEAN S.D. N 22 11.7 10 65 34.8 10 .44 .104 8 1.33 .226 8 20.0 MEAN S.D. N 26 13.2 10 89 37.8 10 .37 .182 10 1.24 .438 10 Covance 6329-183 3M T-6295 267 Table 27 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 32 23.2 9 77 53.0 9 .22 .058 6 .54 .172 6 0.5 MEAN S.D. N 27 17.6 10 70 43.6 10 .20 .065 8 .56 .212 8 2.0 MEAN S.D. N 43 21.2 10 106 44.1 10 .26 .146 10 .63 .234 10 5.0 MEAN S.D. N 16 9.1 10 42 25.2 10 .31 .085 8 .88 .281 8 20.0 MEAN S.D. N 37 40.5 10 69 68.9 10 .35 .197 8 .65 .259 8 Covance 6329-183 3M T-6295 268 Table 28 Summary of Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 22 14.5 10 116 67.1 10 .29 .153 10 1.43 .422 10 0.5 MEAN S.D. N 16 10.0 10 77 28.1 10 .22 .098 10 1.10 .234 10 2.0 MEAN S.D. N 24 9.9 9 93 25.3 9 .35 .192 9 1.27 .333 9 5.0 MEAN S.D. N 21 15.5 10 89 65.7 10 .30 .122 8 1.22 .394 8 20.0 MEAN S.D. N 46 42.4 10 139 114.5 10 .41 .226 8 1.31 .532 8 Covance 6329-183 3M T-6295 269 Table 28 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 18 18.0 10 71 68.2 10 .14 .070 8 .58 .241 8 0.5 MEAN S.D. N 14 9.9 10 74 50.2 10 .16 .055 9 .79 .212 9 2.0 MEAN S.D. N 21 18.1 10 70 49.9 10 .19 .083 10 .66 .158 10 5.0 MEAN S.D. N 18 17.2 10 67 56.7 10 .22 .139 9 .84 .280 9 20.0 MEAN S.D. N 32 37.4 10 96 85.7 10 .21 .119 10 .73 .270 10 Covance 6329-183 3M T-6295 270 Table 29 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 32 15.5 10 133 65.6 10 .34 .141 10 1.30 .390 10 0.5 MEAN S.D. N 23 18.2 10 144 103.4 10 .27 .158 9 1.65 .354 9 2.0 MEAN S.D. N 21 11.4 10 96 30.6 10 .31 .168 10 1.40 .401 10 5.0 MEAN S.D. N 32 21.5 10 168 105.3 10 .27 .115 10 1.41 .228 10 20.0 MEAN S.D. N 46 20.0 9 145 36.4 9 .49 .215 9 1.51 .473 9 Covance 6329-183 3M T-6295 271 Table 29 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL ------ -------- -------- -------- -------- 0 MEAN S.D. N 40 14.8 10 135 42.4 10 .36 .122 10 1.19 .337 10 0.5 MEAN S.D. N 28 13.6 10 132 51.3 10 .22 .163 10 .87 * .278 10 2.0 MEAN S.D. N 23 * 8.8 10 140 69.9 10 .18 .081 10 .99 .307 10 5.0 MEAN S.D. N 37 14.5 10 172 80.9 10 .21 .147 10 .76 * .239 10 20.0 MEAN S.D. N 42 22.7 10 164 85.4 10 .26 .186 9 .93 .226 9 Covance 6329-183 3M T-6295 272 TABLE 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 0.0331 0.0068 0.0151 STANDARD DEV: 55.9 0.0016 0.0010 0.0011 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.0443 0.0159 0.0217 STANDARD DEV: 39.2 0.0073 0.0038 0.0042 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 0.0296 0.0061 0.0133 STANDARD DEV: 51.3 0.0071 0.0011 0.0032 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.0346 0.0115 0.0166 STANDARD DEV: 40.8 0.0062 0.0009 0.0034 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 0.0305 0.0071 0.0133 STANDARD DEV: 31.4 0.0082 0.0023 0.0032 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.0383 0.0146 0.0189 STANDARD DEV: 16.3 0.0060 0.0034 0.0028 --------------------------------------------------------------- --------------------------------------------------------------- 273 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 0.0330 0.0063 0.0146 STANDARD DEV: 44.5 0.0063 0.0008 0.0034 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 0.0250 0.0053 0.0114 STANDARD DEV: 40.1 0.0032 0.0004 0.0005 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.0355 0.0144 0.0172 STANDARD DEV: 17.5 0.0072 0.0026 0.0037 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.0345 0.0140 0.0172 STANDARD DEV: 26.1 0.0043 0.0024 0.0035 --------------------------------------------------------------- 274 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 0.0302 0.0062 0.0137 STANDARD DEV: 55.9 0.0051 0.0013 0.0021 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.0417 0.0150 0.0204 STANDARD DEV: 39.2 0.0093 0.0043 0.0050 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 0.0247 0.0051 0.0112 STANDARD DEV: 51.3 0.0094 0.0016 0.0043 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.0322 0.0108 0.0154 STANDARD DEV: 40.8 0.0056 0.0013 0.0028 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 0.0286 0.0066 0.0126 STANDARD DEV: 31.4 0.0079 0.0019 0.0034 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.0373 0.0141 0.0184 STANDARD DEV: 16.3 0.0052 0.0025 0.0022 --------------------------------------------------------------- --------------------------------------------------------------- 275 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 0.0318 0.0060 0.0140 STANDARD DEV: 44.5 0.0072 0.0010 0.0032 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 0.0253 0.0054 0.0115 STANDARD DEV: 40.1 0.0044 0.0009 0.0012 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.0329 0.0133 0.0159 STANDARD DEV: 17.5 0.0042 0.0013 0.0018 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.0323 0.0131 0.0161 STANDARD DEV: 26.1 0.0052 0.0028 0.0041 --------------------------------------------------------------- 276 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 2.2027 0.4499 1.0000 STANDARD DEV: 55.9 0.0998 0.0620 0.0000 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 2.0506 0.7302 1.0000 STANDARD DEV: 39.2 0.0895 0.0796 0.0000 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 2.2216 0.4652 1.0000 STANDARD DEV: 51.3 0.1022 0.0408 0.0000 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 2.0992 0.7123 1.0000 STANDARD DEV: 40.8 0.1878 0.1139 0.0000 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 2.2768 0.5280 1.0000 STANDARD DEV: 31.4 0.0980 0.0621 0.0000 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 2.0240 0.7647 1.0000 STANDARD DEV: 16.3 0.0532 0.0623 0.0000 --------------------------------------------------------------- --------------------------------------------------------------- 277 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 2.2740 0.4366 1.0000 STANDARD DEV: 44.5 0.1332 0.0471 0.0000 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 2.1876 0.4655 1.0000 STANDARD DEV: 40.1 0.2136 0.0318 0.0000 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 2.0631 0.8388 1.0000 STANDARD DEV: 17.5 0.0817 0.0509 0.0000 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 2.0375 0.8187 1.0000 STANDARD DEV: 26.1 0.2129 0.0414 0.0000 --------------------------------------------------------------- 278 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 1.9158 0.3867 0.8733 STANDARD DEV: 55.9 0.2630 0.0350 0.1428 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 1.1576 0.4123 0.5650 STANDARD DEV: 39.2 0.1245 0.0616 0.0618 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 1.8856 0.3920 0.8470 STANDARD DEV: 51.3 0.2315 0.0241 0.0779 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 1.1245 0.3787 0.5385 STANDARD DEV: 40.8 0.1201 0.0384 0.0695 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 1.8856 0.4356 0.8295 STANDARD DEV: 31.4 0.1583 0.0413 0.0808 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 1.1610 0.4377 0.5741 STANDARD DEV: 16.3 0.0565 0.0231 0.0345 --------------------------------------------------------------- --------------------------------------------------------------- 279 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 2.0510 0.3919 0.9034 STANDARD DEV: 44.5 0.2638 0.0448 0.1208 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 1.9496 0.4174 0.9001 STANDARD DEV: 40.1 0.1517 0.0525 0.1341 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 1.1187 0.4531 0.5420 STANDARD DEV: 17.5 0.1230 0.0345 0.0551 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 1.1562 0.4675 0.5708 STANDARD DEV: 26.1 0.0619 0.0456 0.0446 --------------------------------------------------------------- 280 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 1.8989 0.3849 0.8647 STANDARD DEV: 55.9 0.1660 0.0314 0.0988 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 1.1863 0.4224 0.5792 STANDARD DEV: 39.2 0.1066 0.0567 0.0548 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 1.8373 0.3834 0.8262 STANDARD DEV: 51.3 0.1452 0.0238 0.0350 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 1.1307 0.3796 0.5417 STANDARD DEV: 40.8 0.1396 0.0294 0.0788 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 1.9428 0.4487 0.8551 STANDARD DEV: 31.4 0.1201 0.0321 0.0730 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 1.1545 0.4347 0.5713 STANDARD DEV: 16.3 0.0706 0.0131 0.0472 --------------------------------------------------------------- --------------------------------------------------------------- 281 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 2.0560 0.3930 0.9070 STANDARD DEV: 44.5 0.2362 0.0415 0.1245 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 1.9357 0.4152 0.8945 STANDARD DEV: 40.1 0.1823 0.0634 0.1506 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 1.1446 0.4626 0.5543 STANDARD DEV: 17.5 0.1468 0.0307 0.0637 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 1.1993 0.4844 0.5914 STANDARD DEV: 26.1 0.0960 0.0497 0.0490 --------------------------------------------------------------- 282 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 15.8246 3.2105 7.2089 STANDARD DEV: 55.9 1.1470 0.2501 0.7794 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 9.2939 3.2663 4.5286 STANDARD DEV: 39.2 1.5941 0.2219 0.6977 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 15.5356 3.2119 6.9828 STANDARD DEV: 51.3 2.6697 0.2424 1.1308 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 9.1934 3.0785 4.3942 STANDARD DEV: 40.8 1.2506 0.1365 0.5841 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 14.0264 3.2296 6.1829 STANDARD DEV: 31.4 1.3506 0.1645 0.7912 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 8.4060 3.1686 4.1509 STANDARD DEV: 16.3 0.6870 0.2526 0.2786 --------------------------------------------------------------- --------------------------------------------------------------- 283 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 18.8254 3.5758 8.3121 STANDARD DEV: 44.5 3.0304 0.3075 1.5632 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 20.3339* 4.3329* 9.3597* STANDARD DEV: 40.1 2.2244 0.4281 1.3157 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 8.7074 3.5252 4.2249 STANDARD DEV: 17.5 1.0293 0.2630 0.5225 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 10.5929 4.2760* 5.2213 STANDARD DEV: 26.1 0.6685 0.3527 0.3041 --------------------------------------------------------------- 284 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 1.9239 0.3910 0.8773 STANDARD DEV: 55.9 0.1514 0.0406 0.1103 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 1.4837 0.5274 0.7235 STANDARD DEV: 39.2 0.1221 0.0581 0.0500 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 1.7946 0.3760 0.8099 STANDARD DEV: 51.3 0.1266 0.0428 0.0776 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 1.4581 0.4954 0.6992 STANDARD DEV: 40.8 0.0258 0.0702 0.0644 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 1.8522 0.4291 0.8137 STANDARD DEV: 31.4 0.2526 0.0704 0.1089 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 1.2902* 0.4859 0.6369 STANDARD DEV: 16.3 0.1419 0.0476 0.0603 --------------------------------------------------------------- --------------------------------------------------------------- 285 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 2.6655 0.5072 1.1500 STANDARD DEV: 44.5 1.5011 0.2769 0.5633 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 1.7721 0.3767 0.8107 STANDARD DEV: 40.1 0.1713 0.0158 0.0351 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 1.3227* 0.5390 0.6420 STANDARD DEV: 17.5 0.1112 0.0628 0.0599 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 1.2912* 0.5210 0.6394 STANDARD DEV: 26.1 0.1316 0.0596 0.0970 --------------------------------------------------------------- 286 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 495.5 STANDARD DEV: 55.9 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.0807 0.0292 0.0394 STANDARD DEV: 39.2 0.0147 0.0081 0.0069 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 481.0 STANDARD DEV: 51.3 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.0707 0.0235 0.0339 STANDARD DEV: 40.8 0.0169 0.0027 0.0080 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 434.1 STANDARD DEV: 31.4 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.0674 0.0254 0.0333 STANDARD DEV: 16.3 0.0094 0.0035 0.0049 --------------------------------------------------------------- --------------------------------------------------------------- 287 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 MEAN: 524.2 STANDARD DEV: 44.5 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 470.3 STANDARD DEV: 40.1 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.0768 0.0311 0.0371 STANDARD DEV: 17.5 0.0129 0.0047 0.0058 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.0737 0.0296 0.0362 STANDARD DEV: 26.1 0.0164 0.0056 0.0068 --------------------------------------------------------------- 288 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 495.5 STANDARD DEV: 55.9 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.0731 0.0261 0.0358 STANDARD DEV: 39.2 0.0060 0.0043 0.0040 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 481.0 STANDARD DEV: 51.3 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.0700 0.0233 0.0333 STANDARD DEV: 40.8 0.0180 0.0038 0.0073 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 434.1 STANDARD DEV: 31.4 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.0707 0.0266 0.0349 STANDARD DEV: 16.3 0.0154 0.0053 0.0074 --------------------------------------------------------------- --------------------------------------------------------------- 289 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 MEAN: 524.2 STANDARD DEV: 44.5 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 470.3 STANDARD DEV: 40.1 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.0769 0.0310 0.0373 STANDARD DEV: 17.5 0.0191 0.0066 0.0094 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.0699 0.0280 0.0343 STANDARD DEV: 26.1 0.0086 0.0012 0.0018 --------------------------------------------------------------- 290 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 0.7759 0.1575 0.3526 STANDARD DEV: 55.9 0.0794 0.0174 0.0364 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.5129 0.1822 0.2501 STANDARD DEV: 39.2 0.0444 0.0196 0.0174 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 0.7825 0.1612 0.3510 STANDARD DEV: 51.3 0.1814 0.0229 0.0728 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.5494 0.1845 0.2617 STANDARD DEV: 40.8 0.1033 0.0302 0.0415 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 0.7287 0.1686 0.3201 STANDARD DEV: 31.4 0.0928 0.0250 0.0404 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.5110 0.1926 0.2529 STANDARD DEV: 16.3 0.0445 0.0160 0.0275 --------------------------------------------------------------- --------------------------------------------------------------- 291 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 0.7046 0.1341 0.3120 STANDARD DEV: 44.5 0.1067 0.0120 0.0619 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 0.7754 0.1650 0.3543 STANDARD DEV: 40.1 0.0826 0.0135 0.0103 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.4750 0.1930 0.2303 STANDARD DEV: 17.5 0.0247 0.0099 0.0107 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.4326* 0.1749 0.2141 STANDARD DEV: 26.1 0.0310 0.0182 0.0255 --------------------------------------------------------------- 292 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 1.7498 0.3560 0.7964 STANDARD DEV: 55.9 0.2466 0.0582 0.1237 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 284.0 STANDARD DEV: 39.2 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 1.7146 0.3581 0.7711 STANDARD DEV: 51.3 0.1818 0.0344 0.0617 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 298.9 STANDARD DEV: 40.8 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 1.6809 0.3887 0.7394 STANDARD DEV: 31.4 0.0700 0.0308 0.0461 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 265.7 STANDARD DEV: 16.3 --------------------------------------------------------------- --------------------------------------------------------------- 293 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 1.6548 0.3177 0.7269 STANDARD DEV: 44.5 0.1429 0.0390 0.0220 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 1.6454 0.3504 0.7561 STANDARD DEV: 40.1 0.1290 0.0163 0.0726 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 MEAN: 246.7 STANDARD DEV: 17.5 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 249.1 STANDARD DEV: 26.1 --------------------------------------------------------------- 294 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 1.7446 0.3544 0.7945 STANDARD DEV: 55.9 0.2362 0.0524 0.1246 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 284.0 STANDARD DEV: 39.2 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 1.6989 0.3548 0.7642 STANDARD DEV: 51.3 0.1610 0.0310 0.0531 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 298.9 STANDARD DEV: 40.8 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 1.6716 0.3863 0.7356 STANDARD DEV: 31.4 0.0876 0.0289 0.0557 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 265.7 STANDARD DEV: 16.3 --------------------------------------------------------------- --------------------------------------------------------------- 295 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 1.6615 0.3190 0.7299 STANDARD DEV: 44.5 0.1437 0.0402 0.0263 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 1.6738 0.3567 0.7686 STANDARD DEV: 40.1 0.0932 0.0134 0.0513 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 MEAN: 246.7 STANDARD DEV: 17.5 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 249.1 STANDARD DEV: 26.1 --------------------------------------------------------------- 296 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 0.0162 0.0032 0.0074 STANDARD DEV: 55.9 0.0098 0.0015 0.0046 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.0069 0.0024 0.0033 STANDARD DEV: 39.2 0.0015 0.0004 0.0006 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 0.0121 0.0025 0.0054 STANDARD DEV: 51.3 0.0025 0.0004 0.0009 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.0098 0.0033 0.0047 STANDARD DEV: 40.8 0.0025 0.0006 0.0011 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 0.0140 0.0033 0.0060 STANDARD DEV: 31.4 0.0091 0.0025 0.0036 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.0080 0.0030 0.0040 STANDARD DEV: 16.3 0.0028 0.0011 0.0014 --------------------------------------------------------------- --------------------------------------------------------------- 297 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 0.0143 0.0028 0.0063 STANDARD DEV: 44.5 0.0045 0.0011 0.0020 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 0.0114 0.0025 0.0053 STANDARD DEV: 40.1 0.0016 0.0005 0.0013 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.0091 0.0037* 0.0044 STANDARD DEV: 17.5 0.0008 0.0005 0.0004 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.0077 0.0031* 0.0038 STANDARD DEV: 26.1 0.0012 0.0003 0.0005 --------------------------------------------------------------- 298 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 495.5 0.0171 0.0034 0.0078 STANDARD DEV: 55.9 0.0080 0.0012 0.0038 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.0097 0.0035 0.0047 STANDARD DEV: 39.2 0.0033 0.0015 0.0015 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 481.0 0.0132 0.0027 0.0059 STANDARD DEV: 51.3 0.0038 0.0006 0.0016 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.0110 0.0037 0.0052 STANDARD DEV: 40.8 0.0024 0.0008 0.0008 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 434.1 0.0126 0.0029 0.0055 STANDARD DEV: 31.4 0.0017 0.0005 0.0007 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.0116 0.0044 0.0057 STANDARD DEV: 16.3 0.0062 0.0023 0.0029 --------------------------------------------------------------- --------------------------------------------------------------- 299 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 524.2 0.0159 0.0031 0.0069 STANDARD DEV: 44.5 0.0058 0.0013 0.0023 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 470.3 0.0104 0.0022 0.0048 STANDARD DEV: 40.1 0.0014 0.0004 0.0008 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.0097 0.0039 0.0047 STANDARD DEV: 17.5 0.0013 0.0005 0.0005 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.0095 0.0038 0.0047 STANDARD DEV: 26.1 0.0011 0.0003 0.0004 --------------------------------------------------------------- 300 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 5 MEAN: 495.5 STANDARD DEV: 55.9 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 284.0 0.8644 0.3157 0.4244 STANDARD DEV: 39.2 0.3226 0.1459 0.1667 --------------------------------------------------------------- M2 ------------------------------- NUMBER IN GROUP: 5 MEAN: 481.0 STANDARD DEV: 51.3 --------------------------------------------------------------- F2 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 298.9 0.5986 0.2010 0.2906 STANDARD DEV: 40.8 0.1521 0.0486 0.0974 --------------------------------------------------------------- M3 ------------------------------- NUMBER IN GROUP: 5 MEAN: 434.1 STANDARD DEV: 31.4 --------------------------------------------------------------- F3 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 265.7 0.8368 0.3125 0.4173 STANDARD DEV: 16.3 0.3843 0.1335 0.2021 --------------------------------------------------------------- --------------------------------------------------------------- 301 Table 30 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 14 Sacrifice PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M4 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F4 ------------------------------- NUMBER IN GROUP: 5 MEAN: 524.2 STANDARD DEV: 44.5 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 5 MEAN: 470.3 STANDARD DEV: 40.1 --------------------------------------------------------------- ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 246.7 0.9249 0.3815 0.4478 STANDARD DEV: 17.5 0.3717 0.1685 0.1731 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 5 5 5 5 MEAN: 249.1 0.7984 0.3306 0.3974 STANDARD DEV: 26.1 0.4389 0.1980 0.2223 --------------------------------------------------------------- 302 TABLE 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 0.0313 0.0044 0.0135 STANDARD DEV: 105.5 0.0076 0.0010 0.0036 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 0.0501 0.0129 0.0235 STANDARD DEV: 101.9 0.0290 0.0091 0.0135 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 0.0263 0.0040 0.0112 STANDARD DEV: 142.5 0.0046 0.0008 0.0018 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 0.0311* 0.0095 0.0141* STANDARD DEV: 39.7 0.0053 0.0022 0.0024 --------------------------------------------------------------- --------------------------------------------------------------- 303 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT ADRENAL -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 0.0275 0.0039 0.0119 STANDARD DEV: 105.5 0.0060 0.0010 0.0028 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 0.0368 0.0093 0.0172 STANDARD DEV: 101.9 0.0072 0.0027 0.0032 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 0.0262 0.0040 0.0111 STANDARD DEV: 142.5 0.0031 0.0007 0.0011 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 0.0318 0.0097 0.0144* STANDARD DEV: 39.7 0.0037 0.0016 0.0019 --------------------------------------------------------------- --------------------------------------------------------------- 304 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 2.3308 0.3320 1.0000 STANDARD DEV: 105.5 0.1344 0.0442 0.0000 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 2.1293 0.5376 1.0000 STANDARD DEV: 101.9 0.0884 0.1129 0.0000 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 2.3485 0.3644 1.0000 STANDARD DEV: 142.5 0.0730 0.0722 0.0000 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 2.2148 0.6752* 1.0000 STANDARD DEV: 39.7 0.1036 0.0817 0.0000 --------------------------------------------------------------- --------------------------------------------------------------- 305 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 2.3449 0.3327 1.0078 STANDARD DEV: 105.5 0.1955 0.0397 0.0857 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 1.3621 0.3357 0.6391 STANDARD DEV: 101.9 0.2284 0.0458 0.1000 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 2.4193 0.3712 1.0322 STANDARD DEV: 142.5 0.2614 0.0597 0.1260 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 1.3638 0.4149* 0.6171 STANDARD DEV: 39.7 0.1321 0.0532 0.0668 --------------------------------------------------------------- --------------------------------------------------------------- 306 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT KIDNEY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 2.3577 0.3340 1.0129 STANDARD DEV: 105.5 0.2008 0.0348 0.0842 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 1.4200 0.3498 0.6665 STANDARD DEV: 101.9 0.2361 0.0447 0.1046 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 2.4080 0.3690 1.0271 STANDARD DEV: 142.5 0.2312 0.0529 0.1109 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 1.3769 0.4193* 0.6224 STANDARD DEV: 39.7 0.1375 0.0574 0.0631 --------------------------------------------------------------- --------------------------------------------------------------- 307 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 20.0275 2.8113 8.6130 STANDARD DEV: 105.5 3.0980 0.2044 1.4054 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 11.5769 2.8031 5.4312 STANDARD DEV: 101.9 3.0344 0.3109 1.3763 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 26.6325* 4.0039* 11.3659* STANDARD DEV: 142.5 5.5590 0.5449 2.4751 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 13.8907 4.2047* 6.2883 STANDARD DEV: 39.7 1.8568 0.5156 0.8921 --------------------------------------------------------------- --------------------------------------------------------------- 308 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 2.1599 0.3061 0.9293 STANDARD DEV: 105.5 0.2545 0.0413 0.1174 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 1.6819 0.4215 0.7913 STANDARD DEV: 101.9 0.2502 0.0901 0.1262 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 2.0477 0.3151 0.8719 STANDARD DEV: 142.5 0.1879 0.0524 0.0758 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 1.5740 0.4797 0.7114 STANDARD DEV: 39.7 0.0922 0.0598 0.0433 --------------------------------------------------------------- --------------------------------------------------------------- 309 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 MEAN: 713.4 STANDARD DEV: 105.5 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 0.0562 0.0138 0.0265 STANDARD DEV: 101.9 0.0192 0.0045 0.0092 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 MEAN: 668.6 STANDARD DEV: 142.5 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 0.0557 0.0170 0.0253 STANDARD DEV: 39.7 0.0123 0.0040 0.0061 --------------------------------------------------------------- --------------------------------------------------------------- 310 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT OVARY -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 MEAN: 713.4 STANDARD DEV: 105.5 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 0.1357 0.0321 0.0630 STANDARD DEV: 101.9 0.2404 0.0549 0.1107 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 MEAN: 668.6 STANDARD DEV: 142.5 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 0.0561 0.0171 0.0254 STANDARD DEV: 39.7 0.0129 0.0044 0.0062 --------------------------------------------------------------- --------------------------------------------------------------- 311 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 0.9792 0.1382 0.4208 STANDARD DEV: 105.5 0.0975 0.0100 0.0427 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 0.5577 0.1368 0.2611 STANDARD DEV: 101.9 0.1021 0.0178 0.0419 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 0.8287* 0.1252* 0.3529* STANDARD DEV: 142.5 0.1514 0.0133 0.0635 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 0.5491 0.1650* 0.2468 STANDARD DEV: 39.7 0.1344 0.0306 0.0537 --------------------------------------------------------------- --------------------------------------------------------------- 312 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 1.8551 0.2641 0.7968 STANDARD DEV: 105.5 0.3741 0.0658 0.1645 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 MEAN: 414.5 STANDARD DEV: 101.9 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 1.6414 0.2531 0.6995 STANDARD DEV: 142.5 0.3094 0.0628 0.1325 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 MEAN: 331.9* STANDARD DEV: 39.7 --------------------------------------------------------------- --------------------------------------------------------------- 313 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT TESTIS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 1.8759 0.2683 0.8100 STANDARD DEV: 105.5 0.1801 0.0491 0.1189 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 MEAN: 414.5 STANDARD DEV: 101.9 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 1.6551 0.2560 0.7054 STANDARD DEV: 142.5 0.2786 0.0631 0.1203 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 MEAN: 331.9* STANDARD DEV: 39.7 --------------------------------------------------------------- --------------------------------------------------------------- 314 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 0.0246 0.0035 0.0106 STANDARD DEV: 105.5 0.0060 0.0008 0.0027 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 0.0136 0.0034 0.0065 STANDARD DEV: 101.9 0.0062 0.0018 0.0034 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 0.0195* 0.0029 0.0083* STANDARD DEV: 142.5 0.0041 0.0004 0.0018 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 0.0115 0.0034 0.0052 STANDARD DEV: 39.7 0.0036 0.0010 0.0016 --------------------------------------------------------------- --------------------------------------------------------------- 315 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT THYROID/PARA -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 713.4 0.0249 0.0035 0.0107 STANDARD DEV: 105.5 0.0048 0.0007 0.0018 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 0.0148 0.0037 0.0070 STANDARD DEV: 101.9 0.0039 0.0011 0.0019 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 9 9 9 MEAN: 668.6 0.0240 0.0036 0.0102 STANDARD DEV: 142.5 0.0093 0.0013 0.0040 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 0.0124 0.0038 0.0056 STANDARD DEV: 39.7 0.0046 0.0014 0.0021 --------------------------------------------------------------- --------------------------------------------------------------- 316 Table 31 Summary of Organ Weight Data Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- M1 ------------------------------- NUMBER IN GROUP: 9 MEAN: 713.4 STANDARD DEV: 105.5 -------------------------------- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP (g) (g) (%) RATIO --------------------------------------------------------------- F1 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 414.5 1.0175 0.2557 0.4771 STANDARD DEV: 101.9 0.3207 0.0951 0.1461 --------------------------------------------------------------- M5 ------------------------------- NUMBER IN GROUP: 9 MEAN: 668.6 STANDARD DEV: 142.5 --------------------------------------------------------------- F5 ------------------------------- NUMBER IN GROUP: 10 10 10 10 MEAN: 331.9* 1.0062 0.3111 0.4587 STANDARD DEV: 39.7 0.3090 0.1154 0.1545 --------------------------------------------------------------- --------------------------------------------------------------- 317 TABLE 32 Incidence of Macroscopic Observations Week 14 Sacrifice Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS PAGE: 1 ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN MALOCCLUSION 3143325423 4555555555 0000100000 KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 4 4 4 4 5 5 4 5 CYST(S) LARGE PELVIS(ES) GRANULAR MATERIAL 0000010000 0111100010 0000000010 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 3 5 4 3 3 LUMEN FILLED WITH FLUID 0000020122 THYMUS (TH) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 4 5 5 5 5 5 RED FOCUS(I)/AREA(S) 1000100000 318 TABLE 32 Incidence of Macroscopic Observations Week 14 Sacrifice Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS PAGE: 2 ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 5 5 5 5 5 5 5 5 MISSING/NOT IDENTIFIED 0100000000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 5 5 5 4 5 5 5 4 DARK FOCUS(I)/AREA(S) 0100010001 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 2 5 4 5 5 5 5 5 5 DIFFUSELY RED ** END OF LIST ** 1301000000 319 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 10 9 10 10 NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN MALOCCLUSION ANIMAL OBESE 5312 10 9 10 10 1120 2100 EYE (EY) ............................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 BRAIN (BR) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 COMPRESSED (HYPOTHALAMUS) 0 LIVER (LI) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 DIFFUSELY DARK 0 KIDNEY (KD) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 9 10 010 9 10 10 9 10 9 001 9 10 10 9 10 10 320 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 9 CYST(S) 0001 PANCREAS (PA) ........................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 9 10 MASS(ES) 0010 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 8 10 LARGE CYST(S) 0010 0010 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 321 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 099 LUMEN FILLED WITH FLUID THICKENED WALL 0001 0010 TESTIS (TE) .......................................... NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 9 8 0 0 SMALL 1100 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 322 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 THYMUS (TH) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 LARGE 0 GELATINOUS 0 LUNG (LU) ............................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 9 LIGHT FOCUS(I)/AREA(S) 0 DARK FOCUS(I)/AREA(S) 1 HEART (HT) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 8 10 10 100 100 9 10 10 9 10 8 002 000 9 10 10 9 10 10 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 323 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- TRACHEA (TR) ......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 THYROID (TY) ......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PITUITARY (PI) ....................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 9 LARGE 0 DARK FOCUS(I)/AREA(S) 0 RED FOCUS(I)/AREA(S) 1 RAISED AREA(S) 0 CYST(S) 0 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 864 023 020 002 001 100 9 10 10 9 10 10 324 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 RED FOCUS(I)/AREA(S) 0 THICKENED WALL 0 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 THICKENED WALL 0 DUODENUM (DU) ........................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 7 10 10 100 100 9 10 10 8 10 10 100 9 10 10 9 10 10 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ILEUM (IL) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 325 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 COLON (CO) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 RECTUM (RE) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 SKIN (SK) ............................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 CRUSTED AREA(S) 0 LIGHT AREA(S)/FOCUS(I) 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 MASS(ES) 0 THICKENED 0 CYST(S) 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 8 10 020 010 0 10 10 0 6 10 010 010 020 9 10 10 9 10 10 326 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- CERVIX (CV) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 9 10 MASS(ES) 0010 OVARY (OV) ........................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 9 10 CYST(S) 0010 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 10 10 PROSTATE (PR) ........................................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 327 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 TAIL (TI) ............................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 9 DIFFUSELY RED 0 MOTTLED 1 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 989 021 000 9 10 10 9 10 10 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 328 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, OTHER (LN) ....................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 COAGULATING GL (CG) .................................. NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 329 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 URETER (UE) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 330 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 331 TABLE 33 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Week 53 Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PINNA (PN) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 ** END OF LIST ** 0 10 10 0 10 10 332 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN TAIL - BENT/MISSING BONE MARROW SMEAR TAKEN MALOCCLUSION TEETH MISSING/BROKEN/WORN DISCHARGE-OCULAR ANIMAL THIN STAINS-PERINEUM/PERIANAL FLUID-THORACIC CAVITY GI TRACT-LARGE LUMEN DISCHARGE-NASAL URINARY BLADDER-LARGE LUMEN ANIMAL OBESE DISCHARGE-ORAL AUTOLYSIS-SEVERE FLUID-ABDOMINAL CAVITY PALE MUCOUS MEMBRANES STAINS - SKIN/HAIRCOAT GI TRACT-DARK FLUID CONTENT GI TRACT-DARK DRY CONTENT YELLOWISH MUCOUS MEMBRANES TRACHEA-CONTENT FOAMY MATTER 19 6 9 9 11 0 8 10 9 8 10 0 34 39 39 38 38 29 47 46 44 40 45 33 000001101100 25 15 22 30 35 10 39 20 14 20 40 17 3 6 5 3 5 4 12 11 5 10 5 6 252134231350 11 15 8 16 9 10 11 13 22 9 14 7 5 12 10 5 3 5 12 12 15 13 16 8 342246147231 010011000100 100120001011 330611233160 210230110000 936774212114 140101011000 000000000010 000021000000 000010000000 301431331302 101011101020 000000000100 000000000100 010000000000 333 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- EYE (EY) ............................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 59 60 70 40 70 60 59 60 69 39 OPAQUE CORNEA PROTRUDING LARGE WHITE INTRAOCULAR MATERIAL ROUGHENED CORNEA 001000000001 001000000010 001000000000 010000000000 000000001000 BRAIN (BR) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 45 38 27 36 51 19 32 24 18 22 39 17 COMPRESSED (HYPOTHALAMUS) MASS(ES) RED FOCUS(I)/AREA(S) DEPRESSED AREA(S) 25 20 33 24 19 21 38 36 42 37 31 23 010000000000 000000000100 010000000000 LIVER (LI) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 59 49 42 47 46 22 57 47 49 43 50 27 LARGE MOTTLED DIFFUSELY DARK HEPATIC ANOMALY (LOBULE) LIGHT FOCUS(I)/AREA(S) MASS(ES) ROUGH SURFACE, DIFFUSE 122060011322 212384051521 010010001051 001000000001 0 6 6 4 10 1 3 0 1 5 6 1 002121010230 100011000310 334 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 59 49 42 47 46 22 57 47 49 43 50 27 DIFFUSELY LIGHT RED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) ADHESION(S) 123115111412 5 2 6 5 2 8 12 6 7 4 4 7 000011111111 300000000200 000000000030 000001000000 KIDNEY (KD) .......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 63 48 51 49 54 27 62 54 53 53 66 35 CYST(S) LARGE PELVIS(ES) GRANULAR MATERIAL ROUGH SURFACE, DIFFUSE DIFFUSELY DARK MASS(ES) MOTTLED LARGE DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT LIGHT FOCUS(I)/AREA(S) SEMIFLUID MATERIAL-PELVIS SMALL 124203311100 243586323122 001203023201 3 10 5 6 5 3 0 0 0 2 1 0 000010110211 000011101001 011030000200 220131000100 000001000000 044324211101 100100000000 000001000000 000001000000 335 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SPLEEN (SP) .......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 67 59 59 60 66 34 66 55 59 56 62 38 LARGE SMALL CYST(S) DIFFUSELY LIGHT IRREGULAR SHAPE LIGHT FOCUS(I)/AREA(S) ADHESION(S) MASS(ES) MASS #2 MOTTLED 110021341421 000021000041 000000000020 001001010000 000000100000 100000110000 200001110100 000002000000 000001000000 000000010000 PANCREAS (PA) ........................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 58 56 57 67 36 69 60 58 60 69 40 MASS(ES) DIFFUSELY RED LARGE VESSELS GELATINOUS LIGHT FOCUS(I)/AREA(S) 122132102000 000100000000 102102000000 000000000010 000000000010 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 52 57 58 65 37 52 41 49 43 56 32 LARGE 2 6 3 2 3 0 15 17 7 14 9 6 336 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 52 57 58 65 37 52 41 49 43 56 32 MASS(ES) CYST(S) DIFFUSELY RED SMALL DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREAS(I) MOTTLED IRREGULAR SHAPE MISSING/NOT IDENTIFIED DIFFUSELY DARK 000011120100 010000100100 000001102110 010000101022 000000101101 000000011230 100000131120 000000010000 000001000000 000010100020 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 58 58 58 68 40 68 56 59 56 67 39 LARGE DIFFUSELY MOTTLED DIFFUSELY SMALL MASS(ES) RED DARK 010210010120 010110010220 001000000100 000000221101 001000000000 000010000000 337 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 57 47 45 41 57 30 LUMEN FILLED WITH THICKENED WALL CYST(S) DIFFUSELY RED MASS(ES) LARGE, DIFFUSE FLUID 000000213231 000000232225 0 0 0 0 0 0 9 8 9 11 6 4 000000001000 000000122522 000000010001 TESTIS (TE) .......................................... NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 61 51 52 50 59 35 0 0 0 0 0 0 SMALL CONTAINS FLUID MASS(ES) RED/YELLOW FOCUS(I)/AREA(S) DIFFUSELY RED LARGE MOTTLED 855983000000 143011000000 000001000000 110110000000 000010000000 021020000000 000010000000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 338 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 60 60 70 40 70 60 60 60 70 40 FRACTURE(S) 010000000000 THYMUS (TH) .......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 58 57 59 68 37 68 59 56 58 69 37 RED FOCUS(I)/AREA(S) LARGE MASS(ES) GELATINOUS MISSING/NOT IDENTIFIED 100011002001 000010000000 110000100001 001010100000 212102012211 LUNG (LU) ............................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 57 58 57 67 34 68 56 56 55 55 35 MASS(ES) RED FOCUS(I)/AREA(S) 100102001201 000100010101 339 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 57 58 57 67 34 68 56 56 55 55 35 LIGHT FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) DIFFUSELY RED MOTTLED UNCOLLAPSED DIFFUSELY LIGHT RAISED FOCUS(I)/AREA(S) ADHESION(S) 0 1 1 0 2 1 1 1 1 2 14 2 100010000000 100000100000 110102001000 010003021000 001000000000 000000000001 000000000110 HEART (HT) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 57 58 60 70 37 70 60 60 59 70 40 LARGE DIFFUSELY LIGHT MASS(ES) LIGHT FOCUS(I)/AREA(S) 000001000100 010000000100 010000000000 012002000000 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 60 59 59 69 40 70 60 60 59 69 40 MASS(ES) ATROPHY-DIFFUSE ATROPHY-FOCAL 201110000000 000000000010 000000000100 340 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 TRACHEA (TR) ......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 60 70 40 70 60 60 60 69 40 LARGE MASS(ES) 100000000000 000000000010 THYROID (TY) ......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 58 58 56 66 36 70 57 59 57 69 39 LARGE DIFFUSELY MASS(ES) SMALL DIFFUSELY DIFFUSELY LIGHT DARK RED 422442021311 000000010100 000002010000 100000000000 100000000000 000000000001 341 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 58 60 68 39 70 59 60 60 70 39 LARGE 012021010001 PITUITARY (PI) ....................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 38 30 18 29 38 10 20 14 14 17 23 8 LARGE DARK FOCUS(I)/AREA(S) MASS(ES) RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) DIFFUSELY RED MOTTLED LIGHT FOCUS(I)/AREA(S) 1 2 2 1 2 1 7 5 3 3 12 3 002001231152 25 21 32 25 21 21 38 37 41 37 27 24 444454431572 122110000011 001032001003 100001000000 000000011000 110000000001 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 58 60 70 40 70 60 60 60 69 40 MISSING/NOT IDENTIFIED MASS(ES) MOTTLED 011000000000 000000000010 001000000000 342 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 59 60 60 69 40 SMALL MISSING/NOT IDENTIFIED MASS(ES) DIFFUSELY LIGHT 000000000010 000000010000 001000000000 001000000000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 57 41 44 47 50 23 62 54 56 57 61 33 DARK FOCUS(I)/AREA(S) EROSION/ULCERATION RED FOCUS(I)/AREA(S) THICKENED WALL LIGHT FOCUS(I)/AREA(S) RAISED AREA(S) 4 5 3 3 8 11 4 2 2 3 8 5 213121210001 031220010010 7 13 11 8 12 5 1 1 1 0 0 0 001110111000 000000000001 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 62 56 57 53 65 35 68 55 56 58 67 39 EROSION/ULCERATION THICKENED WALL RED FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) 741645252131 212533002100 000001001000 002000000100 000100000000 343 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- DUODENUM (DU) ........................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 60 60 70 40 RED FOCUS(I)/AREA(S) 000001000000 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 60 70 40 70 60 60 60 69 39 MASS(ES) THICKENED WALL 000000000011 100000000000 ILEUM (IL) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 60 60 60 70 40 70 60 60 59 70 40 DIFFUSELY RED DIVERTICULUM(A) THICKENED WALL 100000000000 000000000100 100000000000 CECUM (CE) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 59 60 60 68 38 70 60 60 60 69 40 RED FOCUS(I)/AREA(S) DIFFUSELY RED THICKENED WALL LARGE MASS(ES) 000011000000 110000000000 010001000000 000000000010 000010000000 344 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COLON (CO) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 69 59 60 60 70 40 MASS(ES) DIVERTICULUM(A) 000000010000 000000100000 RECTUM (RE) .......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 60 70 40 70 60 59 60 70 40 MASS(ES) DIFFUSELY RED 000000001000 100000000000 SKIN (SK) ............................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 64 49 52 52 57 33 63 59 57 58 66 37 ABRASION(S)/ULCERATION(S) CRUSTED AREA(S) LIGHT AREA(S)/FOCUS(I) MASS(ES) RED FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) ALOPECIA-DIFFUSE THICKENED MASS #2 SUBCUTANEOUS EDEMA 010020100010 352456311132 000000100000 374371101010 100000000000 102210100001 000000100101 000000001000 020000000000 000010000000 345 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 29 23 22 24 39 20 MASS(ES) THICKENED MASS #2 CYST(S) MASS #3 MASS #4 MASS #5 MASS #6 MASS #7 MASS #8 0 0 0 0 0 0 28 35 29 28 24 17 0 0 0 0 0 0 15 10 16 13 9 5 0 0 0 0 0 0 14 13 11 14 10 7 0 0 0 0 0 0 13 5 9 4 5 3 000000653622 000000212122 000000012110 000000000010 000000000010 000000000010 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 67 58 59 57 68 39 70 60 60 60 70 40 CALCULUS(I) DIFFUSELY RED SEMIFLUID MATERIAL THICKENED WALL RED FOCUS(I)/AREA(S) 200111000000 100200000000 021000000000 010200000000 000010000000 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 68 57 56 58 66 39 MASS(ES) 000000120021 346 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 68 57 56 58 66 39 FIRM LARGE CYST(S) 000000100000 000000114120 000000000100 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 58 55 51 47 56 29 CYST(S) MASS(ES) LARGE 0 0 0 0 0 0 12 5 9 13 12 11 000000000010 000000000010 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 65 54 53 54 66 35 SEMIFLUID MATERIAL MASS(ES) 000000564545 000000003100 PROSTATE (PR) ........................................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 56 51 44 51 58 33 0 0 0 0 0 0 NODULAR SMALL MASS(ES) LARGE 11 7 12 6 10 6 0 0 0 0 0 0 211110000000 101100000000 014003000000 347 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PROSTATE (PR) ........................................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 56 51 44 51 58 33 0 0 0 0 0 0 GELATINOUS 002111000000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 64 49 49 52 62 33 0 0 0 0 0 0 SMALL LARGE GELATINOUS THICKENED MOTTLED 563551000000 136314000000 023124000000 010000000000 000100000000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 70 60 60 60 68 40 0 0 0 0 0 0 SMALL 000020000000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 69 60 60 60 70 40 MASS(ES) 000000100000 348 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 65 60 56 58 70 38 69 59 59 59 70 40 ABRASION(S)/ULCERATION(S) CRUSTED AREA(S) LARGE DIFFUSELY RED RAISED AREA(S) MASS(ES) 000000001000 101102010000 101000000000 000000100000 201100000000 101000000100 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 65 55 55 58 67 36 58 56 53 56 64 33 DIFFUSELY RED LARGE MOTTLED 144224811246 111010334132 300000203100 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 58 58 58 67 37 68 60 58 59 67 39 LARGE DIFFUSELY RED MOTTLED CYST(S) 012133001030 011022202111 001000000000 000100000000 349 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 57 58 58 67 39 66 53 56 54 68 34 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK 011100233425 110010041202 012221100112 000000100000 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 60 60 70 40 70 58 57 59 69 40 LARGE DIFFUSELY RED 010000023100 000000000010 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 59 59 59 70 40 70 60 60 60 70 40 LARGE DIFFUSELY RED 110100000000 001000000000 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 56 57 57 66 37 69 60 60 59 69 40 LARGE DIFFUSELY RED CYST(S) MOTTLED 022323000110 032130000110 000001000000 000001100000 350 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 64 51 54 54 65 37 0 0 0 0 0 0 THICKENED CYST(S) MASS(ES) MASS #2 010100000000 144012000000 542552000000 200021000000 COAGULATING GL (CG) .................................. NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 70 60 60 59 70 40 0 0 0 0 0 0 LARGE 000100000000 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 58 59 70 37 69 59 58 60 68 40 LARGE DIFFUSELY RED MOTTLED 001002011010 001103111020 010000000000 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 70 59 58 60 69 38 0 0 0 0 0 0 LARGE 012012000000 351 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 57 68 36 68 60 60 60 67 39 MASS(ES) LARGE VESSELS 100222200031 000102000000 AORTA (AO) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 57 58 68 39 70 60 60 59 70 40 MASS(ES) LARGE FIRM 000010000000 013111000100 012101000100 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 58 57 54 66 38 68 57 58 58 65 38 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) LARGE 001432121121 122210111131 100000000000 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 59 60 70 40 70 60 60 60 70 40 MASS(ES) 011000000000 352 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 59 60 70 40 70 60 59 60 70 40 MASS(ES) 011000001000 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 59 57 54 68 34 69 60 56 58 69 38 CYST(S) LARGE DIFFUSELY RED MASS(ES) MOTTLED 010101001001 103413001102 101001002110 000000001000 000111101100 URETER (UE) .......................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 69 60 60 59 70 40 LARGE 000001100100 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 59 57 59 69 37 70 60 60 60 69 40 LARGE 113113000010 353 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 58 60 70 40 69 58 60 60 70 40 MASS(ES) CYST(S) THICKENED GELATINOUS 101000000000 000000100000 001000010000 000000010000 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 70 60 59 60 70 39 0 0 0 0 0 0 LARGE 001001000000 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 69 60 60 60 70 40 MASS(ES) 000000100000 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 59 60 70 39 LARGE DIFFUSELY RED 000001001001 000000001001 354 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 39 70 60 60 60 70 40 MASS(ES) 001001000000 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 LARGE 001000000000 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 LARGE 001000000000 LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 59 70 40 LARGE DIFFUSELY RED 000000000100 000000000100 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 60 60 70 40 LARGE DIFFUSELY RED 000001000000 000001000000 355 TABLE 34 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 THICKENED DIFFUSELY RED 001000000000 001000000000 PINNA (PN) ........................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 60 60 70 39 MASS(ES) 000001000001 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 LARGE 001000000000 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 59 70 40 70 60 60 60 70 40 LARGE 000100000000 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 69 60 60 60 70 40 MASS(ES) ** END OF LIST ** 000000100000 356 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ................................. NUMBER EXAMINED: 10 17 BLOOD FILM TAKEN TAIL - BENT/MISSING BONE MARROW SMEAR TAKEN MALOCCLUSION TEETH MISSING/BROKEN/WORN DISCHARGE-OCULAR ANIMAL THIN ANIMAL OBESE GI TRACT-DARK FLUID CONTENT 10 17 10 10 17 23 20 11 11 12 10 EYE (EY) ............................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 16 OPAQUE CORNEA 01 BRAIN (BR) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 6 10 COMPRESSED (HYPOTHALAMUS) 47 LIVER (LI) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 6 10 LIGHT FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 01 357 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 6 10 RED FOCUS(I)/AREA(S) CYST(S) 46 01 KIDNEY (KD) .......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 7 16 CYST(S) LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE DIFFUSELY LIGHT 20 11 10 10 SPLEEN (SP) .......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 17 ADHESION(S) 10 PANCREAS (PA) ........................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 8 17 MASS(ES) LARGE VESSELS 10 10 358 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 13 LARGE 04 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 16 DIFFUSELY DARK 01 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 11 THICKENED WALL CYST(S) MASS(ES) 03 03 02 TESTIS (TE) .......................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 SMALL 10 359 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 THYMUS (TH) .......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 15 MASS(ES) MISSING/NOT IDENTIFIED 01 11 360 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- LUNG (LU) ............................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 8 15 RED FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) MOTTLED 01 11 10 HEART (HT) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 TRACHEA (TR) ......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 361 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 8 16 LARGE MASS(ES) DIFFUSELY RED 11 10 01 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 16 LARGE 01 PITUITARY (PI) ....................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 2 6 LARGE DARK FOCUS(I)/AREA(S) MASS(ES) RED FOCUS(I)/AREA(S) CYST(S) LIGHT FOCUS(I)/AREA(S) 02 12 47 21 11 01 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 362 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 6 13 DARK FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL 13 11 20 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 DUODENUM (DU) ........................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 16 MASS(ES) 01 ILEUM (IL) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 363 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- CECUM (CE) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 17 THICKENED WALL 10 COLON (CO) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 RECTUM (RE) .......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 SKIN (SK) ............................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 7 15 CRUSTED AREA(S) MASS(ES) RAISED FOCUS(I)/AREA(S) 21 10 01 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 9 MASS(ES) THICKENED MASS #2 MASS #3 ** CONTINUED ON NEXT PAGE ** 08 03 04 01 364 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 9 MASS #4 01 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 16 MASS(ES) 01 OVARY (OV) ........................................... NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 10 CYST(S) 07 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 17 PROSTATE (PR) ........................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 NODULAR 10 365 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 10 0 NOT REMARKABLE: 9 0 SMALL GELATINOUS 10 10 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 TAIL (TI) ............................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 8 17 CRUSTED AREA(S) 20 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 12 DIFFUSELY RED LARGE 14 01 366 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 16 LARGE DIFFUSELY RED 10 01 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 16 LARGE 01 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 17 LARGE MOTTLED 10 10 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 367 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- COAGULATING GL (CG) .................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 10 0 NOT REMARKABLE: 8 0 LARGE 20 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 17 LARGE VESSELS 10 AORTA (AO) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 16 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 10 01 368 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 8 16 CYST(S) LARGE 01 21 URETER (UE) .......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 7 17 LARGE 30 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 369 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 10 0 NOT REMARKABLE: 10 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 17 LARGE 10 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 370 TABLE 35 Incidence of Macroscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 PINNA (PN) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 16 MASS(ES) 01 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 10 17 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 17 ** END OF LIST ** 371 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 5 BRAIN (BR) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 5 LIVER (LI) ........................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 2 0 0 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --NECROSIS, COAGULATIVE 5555535555 0005500014 2201212213 0003401002 2320010321 0000000110 0000000100 LIVER-OIL RED O (LI0) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 1 1 2 1 2 5 2 3 5 --INFILTRATE, LYMPHOHISTIOCYTIC ** CONTINUED ON NEXT PAGE ** 4223230310 372 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 1 1 2 1 2 5 2 3 5 --DILATATION, PELVIC --MINERALIZATION, PELVIC --REGENERATION, TUBULAR EPITHELIUM --MINERALIZATION, TUBULAR 0211200110 0000100010 1013110000 0000000100 SPLEEN (SP) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 3 4 5 4 0 0 1 0 0 --PIGMENT, INCREASED 2210155455 PANCREAS (PA) ........................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 2 5 3 4 4 5 5 3 5 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS 0002100010 0202110010 1100000000 0001000000 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 3 5 5 5 4 5 5 4 5 --VACUOLATION, FOCAL --UNILATERALLY EXAMINED 0100000000 0100010010 373 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 5 5 5 4 5 5 4 5 --UNILATERALLY EXAMINED 0100010010 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 5 5 5 4 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 4 4 5 5 5 5 5 --HEMORRHAGE 0000100000 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 5 5 5 5 5 TESTIS (TE) .......................................... NUMBER EXAMINED: 5 5 5 5 5 0 0 0 0 0 NOT REMARKABLE: 4 5 5 5 5 0 0 0 0 0 --ATROPHY/DEGENERATION 1000000000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 374 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 THYMUS (TH) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LUNG (LU) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEART (HT) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 375 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 THYROID (TY) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PITUITARY (PI) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 376 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 DUODENUM (DU) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ILEUM (IL) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CECUM (CE) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 377 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COLON (CO) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 378 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE 5555555555 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 379 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 VERTEBRA/MARROW (VT) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 380 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COAGULATING GL (CG) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 381 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEAD, CORONAL (HC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 382 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 383 TABLE 36 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 14 Interim Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ** END OF LIST ** 384 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 10 9 10 10 NOT REMARKABLE: 10 9 10 10 BRAIN (BR) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 --COMPRESSION, VENTRAL 0 LIVER (LI) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 0 --INFILTRATE, LYMPHOHISTIOCYTIC 9 --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR 0 POLYKARYOCYTOSIS OCCASIONALLY NOTED --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR 0 --NECROSIS, INDIVIDUAL HEPATOCYTE 0 --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR 1 --VACUOLATION, HEPATOCELLULAR, PERIPORTAL 3 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL 7 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 0 --HYPERPLASIA, BILE DUCT 1 --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL 1 --INFILTRATE, MACROPHAGE, PIGMENTED 2 --VACUOLATION, HEPATOCELLULAR 3 --HYPERTROPHY, HEPATOCELLULAR 0 --CYST, BILIARY 0 --INFLAMMATION, SUBACUTE 0 9 10 10 9 9 10 010 9 10 10 020 569 809 006 233 500 221 000 100 023 000 103 331 100 001 020 385 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LIVER-OIL RED O (LI0) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 KIDNEY (KD) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 0 --NEPHROPATHY, CHRONIC PROGRESSIVE 9 --INFILTRATE, LYMPHOHISTIOCYTIC 0 --DILATATION, PELVIC 0 --MINERALIZATION, PELVIC 1 --REGENERATION, TUBULAR EPITHELIUM 1 --MINERALIZATION, TUBULAR 0 --PIGMENT, TUBULAR 0 --HYALINE DROPLETS, TUBULAR EPITHELIUM 0 --INFILTRATE, MACROPHAGE, PIGMENTED 0 SPLEEN (SP) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 0 --PIGMENT, INCREASED 10 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED 6 --FIBROSIS, CAPSULAR 0 PANCREAS (PA) ........................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 3 --ZYMOGEN GRANULES, DECREASED 1 ** CONTINUED ON NEXT PAGE ** 9 10 10 112 631 101 001 064 000 146 211 100 010 9 10 10 200 7 10 10 252 001 9 10 10 297 000 386 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ........................................ NUMBER EXAMINED: 10 9 10 10 NOT REMARKABLE: 3 2 9 7 --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --NECROSIS / INFLAMMATION, ADIPOSE TISSUE 1012 5701 2000 1101 0010 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 2 9 10 10 210 --UNILATERALLY EXAMINED --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --VACUOLATION, DIFFUSE --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL 0010 6458 0010 2303 0100 0041 0001 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 999 --UNILATERALLY EXAMINED 0011 387 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 8 10 10 --HEMORRHAGE 0100 OVARY (OV) ........................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 9 10 --CYST 0010 TESTIS (TE) .......................................... NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 8 9 0 0 --ATROPHY/DEGENERATION --GRANULOMA, SPERM 2000 1000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 5 --HYPOCELLULAR, MARROW 5 --HYPERPLASIA, MYELOID, MARROW 0 9 10 10 6 10 8 301 001 388 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 9 9 10 10 9 10 9 --HYPERPLASIA, MYELOID, MARROW --HYPOCELLULAR, MARROW 0001 1000 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 THYMUS (TH) .......................................... NUMBER EXAMINED: 7 NOT REMARKABLE: 5 --HEMORRHAGE 2 --DEPLETION, LYMPHOCYTIC 2 (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --M-THYMIC CARCINOMA 0 --CYST 0 LUNG (LU) ............................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 6 --CONGESTION 1 --MINERALIZATION, VASCULAR 1 ** CONTINUED ON NEXT PAGE ** 8 10 9 276 322 310 100 001 9 10 10 554 000 310 389 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 10 9 10 10 NOT REMARKABLE: 6 5 5 4 --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC 1121 0124 1132 HEART (HT) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 7 9 10 10 5 8 10 --CARDIOMYOPATHY --HEMORRHAGE 3420 0100 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 TRACHEA (TR) ......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 10 9 9 10 NOT REMARKABLE: 10 9 9 10 390 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- THYROID (TY) ......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 8 9 10 10 9 10 10 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL 1000 1000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PITUITARY (PI) ....................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 4 --B-ADENOMA 4 --HYPERPLASIA 2 --ANGIECTASIS 1 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 --INFILTRATE, LYMPHOHISTIOCYTIC 0 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 855 142 114 033 9 10 10 899 111 9 10 10 9 10 10 391 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 9 9 10 10 7 8 10 --EDEMA --INFLAMMATION, ACUTE --CONGESTION --EROSION 0010 1020 0100 0100 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 9 9 10 10 9 10 10 --ACANTHOSIS / HYPERKERATOSIS 1000 DUODENUM (DU) ........................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ILEUM (IL) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 CECUM (CE) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 392 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- COLON (CO) ........................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 RECTUM (RE) .......................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 --PARASITISM 0 SKIN (SK) ............................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 --ACANTHOSIS/HYPERKERATOSIS 0 --INFLAMMATION, CHRONIC-ACTIVE 0 --ULCERATION 0 --HEMORRHAGE 0 --DEGENERATION, MUSCLE, SUBCUTANEOUS 0 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 --CYST / GALACTOCELE 0 --HYPERPLASIA, GLANDULAR 0 --SECRETORY MATERIAL 0 --M-FIBROSARCOMA 0 9 10 10 7 10 10 200 9 10 10 9 8 10 020 020 020 010 010 0 10 10 032 032 044 065 010 393 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 10 9 10 9 NOT REMARKABLE: 7 7 10 8 --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL 3001 0200 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 10 7 --DILATATION 0003 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 10 10 0 9 10 --CYST, SQUAMOUS 0010 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 10 9 NOT REMARKABLE: 0 0 10 9 PROSTATE (PR) ........................................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 4 6 0 0 --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, LYMPHOHISTIOCYTIC 5300 1000 394 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 9 --IMMATURE SPERM FORMS 0 --INFILTRATE, LYMPHOHISTIOCYTIC 1 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 900 700 100 100 9 10 10 9 10 10 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 0 --SCHEDULED SACRIFICE 10 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 9 10 10 000 9 10 10 000 000 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 395 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 1 0 NOT REMARKABLE: 0 0 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE 0010 0010 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 1 0 2 1 NOT REMARKABLE: 0 0 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE 0020 1021 VERTEBRA/MARROW (VT) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 396 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- COAGULATING GL (CG) .................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TAIL (TI) ............................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 397 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HEAD, CORONAL (HC) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 398 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 399 TABLE 37 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Week 53 Interim Sacrifice PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ------------------------------------------------------------------------ -=- -=- -=- -=- PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ** END OF LIST ** 400 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 9 0 0 0 20 22 0 0 0 23 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION --DEGENERATION, RETINAL 2000210002 0000010000 BRAIN (BR) ........................................... NUMBER EXAMINED: 11 0 7 5 22 24 8 5 8 25 NOT REMARKABLE: 9 0 3 0 14 11 1 2 1 14 --DILATATION, VENTRICULAR --COMPRESSION, VENTRAL --HEMORRHAGE --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --I-CARCINOMA 0000122101 2 0 4 5 6 10 7 3 5 11 0000010000 0010110011 0000100000 0000010010 LIVER (LI) ........................................... NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 0 0 0 0 0 0 0 9 15 25 000 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL 11 10 13 23 20 17 14 0 1 3 13 18 1 0 0000900 0000300 1142712 0057800 0054375 6 12 23 1 7 20 0 6 20 0 1 17 224 002 6 4 11 401 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 11 10 17 25 22 24 15 9 15 25 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL --INFILTRATE, MACROPHAGE, PIGMENTED --VACUOLATION, HEPATOCELLULAR --DEGENERATION / NECROSIS, CENTRILOBULAR --CYST, BILIARY --ANGIECTASIS --B-ADENOMA, HEPATOCELLULAR --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --N-CARCINOMA 7 7 10 20 17 18 6 6 8 15 9 3 12 16 10 11 11 5 10 15 3 4 8 13 14 0 0 1 0 1 0100100112 0000010000 0000100000 1021322446 8 5 8 13 15 14 10 3 7 14 4244877235 0020091122 0 0 0 0 1 1 0 0 1 11 2100000001 0001000000 0000010002 0010141210 0110601014 0021010020 0001000000 LIVER-OIL RED O (LI0) ................................ NUMBER EXAMINED: 5 0 0 0 5 5 0 0 0 5 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR 5000550005 402 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 1 0 1 0 0 0 2 9 15 25 120 --NEPHROPATHY, CHRONIC PROGRESSIVE --DILATATION, PELVIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --VACUOLATION, TUBULAR EPITHELIUM --HYALINE DROPLETS, TUBULAR EPITHELIUM --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --INFLAMMATION, CHRONIC-ACTIVE, PELVIC --ECTASIA, TUBULAR --CYST --INFLAMMATION, CHRONIC-ACTIVE --M-CARCINOMA, TUBULAR CELL --THROMBUS --B-LIPOMA --M-RENAL MESENCHYMAL TUMOR --DEGENERATION TUBULAR --INFARCT 10 10 16 24 22 21 11 0000220 2 0 3 5 1 18 11 0001032 0000010 0000100 1013032 1001011 0110000 0001000 0202010 0000110 0000010 0002100 0000110 0010000 0000010 0000010 8 10 22 001 6 12 18 104 000 000 113 110 000 000 000 000 000 000 000 000 000 000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 1 25 NOT REMARKABLE: 1 0 0 0 6 0 0 0 0 1 --PIGMENT, INCREASED --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 4 0 0 0 6 15 0 0 0 18 8 0 0 0 13 21 0 0 1 17 0000100000 403 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SPLEEN (SP) .......................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 1 25 NOT REMARKABLE: 1 0 0 0 6 0 0 0 0 1 --HYPERPLASIA, LYMPHORETICULAR 0000000010 PANCREAS (PA) ........................................ NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 3 1 1 3 3 14 10 9 15 25 6 5 13 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR --FIBROSIS --ECTOPIC HEPATOCYTES --B-FIBROMA --INFLAMMATION, CHRONIC-ACTIVE 1134712114 0012000000 2143110000 0110100000 2334402112 1100211010 0015400000 0122221102 6 6 13 10 6 6 2 2 7 5 1003100000 0000000001 0001000000 0000000001 0000000101 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 11 2 0 1 22 24 5 1 5 25 NOT REMARKABLE: 1 0 0 0 2 0 0 0 0 2 --VACUOLATION, FOCAL --ANGIECTASIS 2000840001 1 1 0 0 0 14 1 0 1 6 404 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 11 2 0 1 22 24 5 1 5 25 NOT REMARKABLE: 1 0 0 0 2 0 0 0 0 2 --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERTROPHY, CORTICAL CELL --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL --PIGMENT --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --CORTICAL TISSUE, EXTRACAPSULAR 0000010100 3 0 0 0 8 16 4 1 3 16 1 0 0 0 1 11 3 1 3 13 2000340011 9 0 0 1 13 22 5 1 4 23 1000100011 0000100000 0100000000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 11 2 0 1 22 24 5 1 5 25 NOT REMARKABLE: 8 1 0 0 15 18 3 0 2 19 --UNILATERALLY EXAMINED --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 1000131123 2000310001 1101231013 0000100000 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 11 0 0 1 22 24 3 0 0 25 NOT REMARKABLE: 10 0 0 0 20 23 1 0 0 23 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, VASCULAR 1000011001 0000100000 0001100000 0000011000 0001000000 405 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 11 0 0 1 22 24 3 0 0 25 NOT REMARKABLE: 10 0 0 0 20 23 1 0 0 23 --LYMPHANGIECTASIS 0000000001 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 24 2 2 6 25 NOT REMARKABLE: 0 0 0 0 0 15 0 1 0 12 --CYST --HYPERPLASIA, GRANULOSA CELL --UNILATERALLY EXAMINED 0 0 0 0 0 8 2 0 6 10 0000020005 0000000102 TESTIS (TE) .......................................... NUMBER EXAMINED: 11 10 17 25 22 0 0 0 0 0 NOT REMARKABLE: 9 4 10 17 15 0 0 0 0 0 --ATROPHY/DEGENERATION --INFLAMMATION, VASCULAR --MINERALIZATION, VASCULAR --HYPERPLASIA, INTERSTITIAL CELL --B-INTERSTITIAL CELL TUMOR --INFILTRATE, LYMPHOHISTIOCYTIC (LEYDIG CELL) 2454500000 0213000000 0101100000 0010100000 0122000000 0010100000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 25 406 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 21 14 0 0 0 18 --HYPOCELLULAR, MARROW --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW 0000150002 0000050004 0000000001 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 22 19 0 0 0 21 --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW 0000050003 0000000001 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 21 23 0 0 0 24 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, CARTILAGINOUS 0000100000 0000010001 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 22 23 0 0 0 25 --DEGENERATION, AXONAL --INFILTRATE, LYMPHOHISTIOCYTIC 0000010000 0000010000 407 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- THYMUS (TH) .......................................... NUMBER EXAMINED: 9 0 0 1 19 22 0 0 0 25 NOT REMARKABLE: 2 0 0 0 1 4 0 0 0 5 --DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --HYPERPLASIA, LYMPHORETICULAR 7 0 0 1 18 17 0 0 0 20 0000010000 LUNG (LU) ............................................ NUMBER EXAMINED: 11 10 17 24 22 24 15 NOT REMARKABLE: 2 7 10 11 10 14 8 9 15 25 488 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --MINERALIZATION, VASCULAR --N-CARCINOMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERPLASIA, TYPE II PNEUMOCYTE --N-SARCOMA --FIBROSIS, PLEURAL/SUBPLEURAL 0000100000 6 1 5 10 7 3 1 1 5 7 0001011001 0000100000 4 2 1 1 6 6 4 5 4 12 2020121025 1020201011 0001000000 0000000001 HEART (HT) ........................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 0 0 0 0 1 12 0 0 0 11 --CARDIOMYOPATHY --MINERALIZATION, VASCULAR --M-ENDOCARDIAL SCHWANNOMA 11 0 0 0 21 12 0 0 0 13 0000100000 0000000001 408 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 11 0 0 0 22 24 0 0 1 25 NOT REMARKABLE: 10 0 0 0 20 24 0 0 1 25 --DEGENERATION --ATROPHY --M-SARCOMA, UNDIFFERENTIATED 0000100000 0000100000 1000000000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 21 24 0 0 0 25 --DEGENERATION 0000100000 TRACHEA (TR) ......................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 21 24 0 0 0 25 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000100000 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 21 24 0 0 0 25 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000100000 THYROID (TY) ......................................... NUMBER EXAMINED: 11 10 17 24 22 24 15 NOT REMARKABLE: 4 3 5 5 6 10 4 9 15 25 049 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR 3 3 6 5 7 10 8 6 9 13 1012000010 2126400000 409 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ......................................... NUMBER EXAMINED: 11 10 17 24 22 24 15 9 15 25 NOT REMARKABLE: 4 3 5 5 6 10 4 0 4 9 --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --HYPERTROPHY, FOLLICULAR CELL --M-CARCINOMA, C-CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --B-HIBERNOMA --COLLOID, DECREASED 1239363214 0222400011 0010000000 0010001000 0000100000 0000000001 0000000100 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 11 10 17 24 22 24 14 NOT REMARKABLE: 9 9 16 19 20 24 14 9 15 24 9 14 23 --HYPERPLASIA --B-ADENOMA 1104200010 1011000001 PITUITARY (PI) ....................................... NUMBER EXAMINED: 11 NOT REMARKABLE: 2 5 13 11 22 24 15 000210 6 11 25 004 --B-ADENOMA --HYPERPLASIA --ANGIECTASIS --CYST --M-CARCINOMA 7 2 11 11 17 16 10 4 7 17 3 3 4 2 6 10 6 2 4 6 0000001102 0011000012 0000000010 410 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 11 0 1 0 22 24 0 0 0 25 NOT REMARKABLE: 9 0 1 0 20 21 0 0 0 22 --INFILTRATE, LYMPHOHISTIOCYTIC 2000230003 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 25 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 11 6 6 5 22 24 2 1 0 25 NOT REMARKABLE: 7 1 0 3 14 23 2 0 0 23 --EDEMA --INFLAMMATION, ACUTE --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR --MINERALIZATION, GLANDULAR MUCOSA --FIBROSIS 0100000000 0110010101 0002200101 2011100001 0010000001 4460600000 0000100000 0000100000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 11 0 0 0 22 24 1 0 0 25 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 24 --ACANTHOSIS / HYPERKERATOSIS --ULCERATION --INFLAMMATION, CHRONIC-ACTIVE 0000001001 0000000001 0000001001 411 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- DUODENUM (DU) ........................................ NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 10 0 0 0 22 24 0 0 0 25 --INFLAMMATION, CHRONIC-ACTIVE 1000000000 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 25 ILEUM (IL) ........................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 10 0 0 0 22 23 0 0 0 23 --INFLAMMATION, CHRONIC-ACTIVE --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 1000000001 0000010001 CECUM (CE) ........................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 10 0 0 0 22 24 0 0 0 24 --INFLAMMATION, CHRONIC-ACTIVE 1000000001 COLON (CO) ........................................... NUMBER EXAMINED: 11 0 0 0 22 24 1 0 0 25 NOT REMARKABLE: 8 0 0 0 22 23 0 0 0 24 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE --DIVERTICULUM --B-LEIOMYOMA 2000000000 1000000001 0000010000 0000001000 412 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- RECTUM (RE) .......................................... NUMBER EXAMINED: 11 0 0 0 22 23 0 0 0 25 NOT REMARKABLE: 9 0 0 0 21 23 0 0 0 20 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE 1000100004 1000000001 SKIN (SK) ............................................ NUMBER EXAMINED: 11 3 4 6 22 24 1 0 0 25 NOT REMARKABLE: 10 0 0 0 15 23 0 0 0 21 --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --CYST, EPIDERMAL INCLUSION --SUPERFICIAL CRUSTING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --B-KERATOACANTHOMA --M-FIBROSARCOMA --B-FIBROMA --FIBROSIS --B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS --B-LIPOMA --M-CARCINOMA, SQUAMOUS CELL --EDEMA 1001000001 1101201001 0001000001 0000001000 1000200001 1000100000 0000100000 0213300000 0001000000 0121000001 0101100000 0010010000 0000101001 0100000000 0000100000 413 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 24 15 9 15 25 NOT REMARKABLE: 0 0 0 0 0 0 1 1 0 1 --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --M-FIBROSARCOMA --B-ADENOMA --FIBROSIS 0000051116 0 0 0 0 0 13 9 3 6 11 0000020000 0 0 0 0 0 12 9 5 6 7 0 0 0 0 0 19 10 6 12 21 0000000011 0000032121 0000010000 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 10 5 13 17 17 23 11 9 15 25 9 15 23 --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC-ACTIVE --DIVERTICULUM 0314004002 1234400000 0013100000 0001000000 0000010000 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 24 4 3 6 25 NOT REMARKABLE: 0 0 0 0 0 14 0 1 0 13 --DILATATION --B-POLYP, ENDOMETRIAL, STROMAL --HYPERPLASIA, CYSTIC, ENDOMETRIAL --HEMORRHAGE 0000041022 0000001003 0000062238 0000000010 414 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 24 4 3 6 25 NOT REMARKABLE: 0 0 0 0 0 14 0 1 0 13 --B-ADENOMA 0000000010 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 24 0 2 2 25 NOT REMARKABLE: 0 0 0 0 0 21 0 0 0 21 --HYPERPLASIA, MUCINOUS --HYPERPLASIA, STROMAL --M-SARCOMA, STROMAL --HYPERPLASIA, CYSTIC --HEMORRHAGE 0000030112 0000000100 0000000002 0000000010 0000000001 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 24 0 1 1 25 NOT REMARKABLE: 0 0 0 0 0 9 0 0 0 13 --LUMEN, EXUDATE --HYPERPLASIA, MUCINOUS --I-CARCINOMA 0000040004 0 0 0 0 0 11 0 1 1 8 0000010000 PROSTATE (PR) ........................................ NUMBER EXAMINED: 11 1 5 4 22 0 0 0 0 0 NOT REMARKABLE: 2 0 0 0 5 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --HYPERPLASIA, EPITHELIAL 9 1 5 4 17 0 0 0 0 0 0001000000 415 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 11 0 1 1 22 0 0 0 0 0 NOT REMARKABLE: 8 0 1 0 18 0 0 0 0 0 --SECRETION, DECREASED --SECRETION, INCREASED --INFLAMMATION, CHRONIC-ACTIVE 2000000000 1000100000 0001300000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 11 0 0 0 22 0 0 0 0 0 NOT REMARKABLE: 9 0 0 0 19 0 0 0 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPOSPERMIA --VACUOLATION, TUBULAR EPITHELIUM 2000000000 0000300000 0000100000 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 11 0 0 0 20 24 0 0 0 25 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 0000100000 0000100000 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 0 0 0 0 0 0 0 9 15 25 000 --SCHEDULED SACRIFICE 11 10 17 25 22 24 15 9 15 25 416 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 0 0 2 0 1 0 0 0 2 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --LYMPHANGIECTASIS 0000000001 0001010001 0002000001 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 4 2 2 3 6 7 4 2 5 6 NOT REMARKABLE: 3 0 0 2 4 3 0 1 1 2 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 0000100000 0011120011 0221124144 1001012010 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 1 0 0 0 3 1 0 0 1 NOT REMARKABLE: 0 1 0 0 0 2 0 0 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --N-CARCINOMA 0000001000 0000000001 0000000001 0000010000 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0000000001 0000000001 417 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 1 0 1 2 2 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --DILATATION, SINUSOIDAL 0001010000 0100210000 0000010000 0000200000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 2 1 1 2 2 5 2 0 3 3 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 2 --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --LYMPHANGIECTASIS --INFILTRATE, MACROPHAGE, PIGMENTED 2111231021 1001251010 1000000000 0000100000 VERTEBRA/MARROW (VT) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 2 1 1 1 2 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --M-CARCINOMA --B-ADENOMA 1110100000 2000000000 0001100000 1000000000 0000100000 418 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COAGULATING GL (CG) .................................. NUMBER EXAMINED: 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE 1000000000 TAIL (TI) ............................................ NUMBER EXAMINED: 3 0 0 1 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --CYST, EPIDERMAL INCLUSION --ACANTHOSIS / HYPERKERATOSIS --B-KERATOACANTHOMA --M-LIPOSARCOMA 0000000010 2000000000 1000000000 0001000000 0000000010 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 0 1 4 0 1 1 2 4 7 NOT REMARKABLE: 0 0 0 0 0 0 0 0 2 1 --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --N-CARCINOMA 1013000000 0013001225 1000000002 1000000010 0000010000 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 1 0 0 1 0 1 1 0 0 3 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION / NECROSIS, FAT --INFLAMMATION, CHRONIC-ACTIVE 1000001003 1000000000 419 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 1 0 0 1 0 1 1 0 0 3 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, VASCULAR --THROMBUS --M-LIPOSARCOMA 0001000000 0001000000 0000010000 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 0010000000 0010000000 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 0 2 3 3 0 0 2 1 0 4 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --ACANTHOSIS / HYPERKERATOSIS --FIBROSIS --BACTERIA --HYPERPLASIA, BONE 0233002104 0232001102 0133002103 0000001001 0101000001 0000000001 420 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 0 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 0000100000 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 0 1 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 1 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 0010000000 0010000000 0010000000 HEAD, CORONAL (HC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 1 1 1 1 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS 1111000001 1000000000 421 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --B-AORTIC BODY TUMOR 1000000000 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 422 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 423 TABLE 38 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Terminal Sacrifice PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ----------MALE--------- ---------FEMALE-------GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 0 0 0 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --GANGLION ** END OF LIST ** 0001000000 424 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 39 39 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 34 36 28 24 26 28 23 30 37 30 21 22 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION --INFLAMMATION, ACUTE --I-NEUROFIBROSARCOMA --ATROPHY, RETINAL --DEGENERATION, LENTICULAR --INFLAMMATION, CHRONIC-ACTIVE --UNILATERALLY EXAMINED --ULCERATION, CORNEAL 413122312310 100010231231 001000000000 001000000100 010000010000 010000001010 000000001000 000000001010 BRAIN (BR) ........................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 19 6 5 15 16 7 9 6 9 8 8 --DILATATION, VENTRICULAR --M-OLIGODENDROGLIOMA --COMPRESSION, VENTRAL --HEMORRHAGE --M-ASTROCYTOMA --DEGENERATION, FOCAL --MINERALIZATION --INFILTRATE, MACROPHAGE, PIGMENTED --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --M-GRANULAR CELL TUMOR --I-CARCINOMA 13 12 14 10 7 6 14 12 19 11 13 10 100000000000 18 15 22 18 12 13 19 26 35 23 16 15 201110311101 200100000100 001000000000 013110001100 120000110201 000000010000 100000000000 010000000000 000000001100 425 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** BRAIN (BR) ........................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 19 6 5 15 16 7 9 6 9 8 8 --M-MENINGEAL SARCOMA --M-NEUROFIBROSARCOMA 010000000000 001000000000 LIVER (LI) ........................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 2 0 0 0 0 1 2 1 1 1 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL --INFILTRATE, MACROPHAGE, PIGMENTED 26 28 26 17 15 22 16 23 27 24 19 16 0 1 1 4 11 3 1 1 3 8 19 2 0 0 0 0 5 0 0 0 0 1 16 1 0 0 0 0 3 0 0 0 0 0 13 0 212233222351 231323100101 10 11 7 12 10 7 5 10 13 10 9 5 6 14 13 4 7 11 9 15 11 8 6 5 1 4 4 1 2 3 5 9 8 11 5 4 2 11 11 4 8 12 0 1 0 2 3 0 131613322101 111110121000 220021001101 3 3 6 2 4 5 8 15 11 4 1 4 11 15 17 11 10 13 7 15 16 10 13 8 457361262651 315223398213 453432135596 426 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 2 0 0 0 0 1 2 1 1 1 0 0 --VACUOLATION, HEPATOCELLULAR --DEGENERATION / NECROSIS, MIDZONAL --DEGENERATION / NECROSIS, CENTRILOBULAR --HEMORRHAGE --CYST, BILIARY --INFLAMMATION, SUBACUTE --GLYCOGEN, HEPATOCELLULAR, INCREASED --ANOMALY, SECONDARY TO DIAPHRAGMATIC HERNIA --ANGIECTASIS --LEUKOCYTOSIS, SINUSOIDAL --B-ADENOMA, HEPATOCELLULAR --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, BILE DUCT --STASIS, BILE --MITOSIS, HEPATOCELLULAR, INCREASED --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --N-SARCOMA --B-CHOLANGIOMA --N-CARCINOMA --KARYOMEGALY --M-CARCINOMA, HEPATOCELLULAR 113141020311 000010000000 100051010221 001000010100 010101211110 010000000010 130010000000 001000000001 111010200010 000010010000 022110001011 000111000110 000000000010 000000000010 000010011000 001000010010 000001000000 000000000100 000010000000 010000000000 000000000010 LIVER-OIL RED O (LI0) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 427 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) .......................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 1 3 0 2 0 3 1 5 4 0 1 0 --NEPHROPATHY, CHRONIC PROGRESSIVE --INFILTRATE, LYMPHOHISTIOCYTIC --DILATATION, PELVIC --MINERALIZATION, PELVIC --REGENERATION, TUBULAR EPITHELIUM --MINERALIZATION, TUBULAR --HEMATOPOIESIS, EXTRAMEDULLARY --ATROPHY, TUBULAR --VACUOLATION, TUBULAR EPITHELIUM --N-CARCINOMA --PIGMENT, TUBULAR --KARYOMEGALY, TUBULAR CELL --HYALINE DROPLETS, TUBULAR EPITHELIUM --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --INFLAMMATION, CHRONIC-ACTIVE, PELVIC --NECROSIS, PAPILLARY --ECTASIA, TUBULAR --CAST, PROTEINACEOUS --CYST --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS, CORTICAL --ANGIECTASIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 30 33 33 21 22 27 11 18 18 18 17 13 100010011110 333167023011 9 7 7 5 5 4 23 23 30 29 14 18 120111010000 200031769795 010000011100 000000001000 000020010210 000000000001 310010002131 000000010000 510120000000 201000000010 022213387622 023202024121 122111010021 000020000000 110000000010 000000000010 010001011000 100100000000 100000000000 000001000000 100000000000 428 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 1 3 0 2 0 3 1 5 4 0 1 0 --BACTERIAL COLONIES --DEGENERATION, CYSTIC, UNILATERAL --INFILTRATE, FAT, INTERSTITIAL --N-SARCOMA --M-CARCINOMA, TUBULAR CELL --ULCERATION, TRANSITIONAL EPITHELIUM, --M-LIPOSARCOMA --THROMBUS --B-LIPOMA --THROMBUS, GLOMERULAR --B-PAPILLOMA, TRANSITIONAL CELL --DEGENERATION TUBULAR PELVIC 000100000000 000000000100 000000001000 100000000000 100000000000 000001000000 000001000000 001000000100 000000001000 000010000000 000001000000 000010000000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 5 4 2 3 2 6 2 0 0 0 3 0 --PIGMENT, INCREASED --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --DEPLETION, LYMPHOCYTIC --FIBROSIS, CAPSULAR --CYST, SEROSAL --M-OSTEOSARCOMA --HYPERPLASIA, LYMPHORETICULAR --M-HEMANGIOSARCOMA 23 27 22 16 16 14 18 18 31 23 18 14 10 16 15 9 12 12 13 23 16 15 4 15 110010000100 000010001001 100000101000 000000000010 000001000000 000000000001 000001000000 429 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SPLEEN (SP) .......................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 5 4 2 3 2 6 2 0 0 0 3 0 --HEMORRHAGE --NECROSIS 000000010000 000000010000 PANCREAS (PA) ........................................ NUMBER EXAMINED: 39 39 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 11 11 6 6 8 7 16 17 21 23 15 10 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --B-ADENOMA, ACINAR CELL --VACUOLATION, FOCAL --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFILTRATE, EOSINOPHILIC --FIBROSIS --INFLAMMATION, GRANULOMATOUS --NECROSIS 9 14 8 8 8 7 2 9 13 7 8 8 100001102211 16 15 14 10 11 13 0 2 1 1 0 0 112111210000 445522214113 120111042001 121012101010 422220010000 010000000000 000000010000 5 10 7 4 6 3 4 5 2 2 1 3 114103011001 100002000000 000001001000 001000000000 001000000100 001000000000 000010000000 430 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 10 5 8 7 9 8 1 2 3 2 1 1 --VACUOLATION, FOCAL --UNILATERALLY EXAMINED --CONGESTION --ANGIECTASIS --HEMATOPOIESIS, EXTRAMEDULLARY --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --VACUOLATION, DIFFUSE --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL --PIGMENT --MINERALIZATION --ATROPHY, CORTICAL --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --DEGENERATION / NECROSIS, UNILATERAL --NECROSIS, FOCAL --FIBROSIS --N-SARCOMA --M-CARCINOMA 7 14 10 3 7 7 4 0 5 3 2 3 100001010100 100000100100 2 2 3 1 1 2 7 15 24 14 7 9 101003153510 000000001030 000000010200 5 5 3 2 5 7 10 13 10 13 8 11 121100000000 1 3 2 0 1 0 10 13 8 14 6 8 222211043432 14 26 16 14 13 14 14 23 27 21 17 13 100001000000 011110010100 201200111110 100000000000 001010011010 001000000000 001001000010 000001000000 000010010000 431 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 34 28 22 21 23 26 23 25 37 27 21 21 --UNILATERALLY EXAMINED --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 221111132420 158132020202 255231242220 120000010000 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 38 38 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 35 33 31 22 26 29 26 33 39 31 24 22 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --M-HEMANGIOSARCOMA --HYPERPLASIA, LYMPHOPLASMACYTIC --MINERALIZATION, VASCULAR --HYPERPLASIA, RETICULOENDOTHELIAL --HYPERPLASIA, LYMPHORETICULAR --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, PIGMENTED --B-HEMANGIOMA --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS --ANGIECTASIS 210100000111 010010000100 000100000000 000001010110 131110000000 000000001000 000000001000 001000000000 001000000000 000000010000 000000000100 000010000000 000010000000 000010000000 432 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 22 31 34 29 20 17 --CYST --HEMATOMA --B-GRANULOSA/THECA CELL TUMOR --HYPERPLASIA, GRANULOSA CELL --CORPORA LUTEA, PERSISTENT 000000347634 000000000010 000000100000 000000000011 000000000001 TESTIS (TE) .......................................... NUMBER EXAMINED: 39 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 28 23 23 19 20 19 0 0 0 0 0 0 --ATROPHY/DEGENERATION --GRANULOMA, SPERM --UNILATERALLY EXAMINED --INFLAMMATION, VASCULAR --M-HEMANGIOSARCOMA --MINERALIZATION, VASCULAR --HYPERPLASIA, INTERSTITIAL CELL (LEYDIG --B-INTERSTITIAL CELL TUMOR --M-MALIGNANT INTERSTITIAL CELL TUMOR --INFILTRATE, LYMPHOHISTIOCYTIC CELL) 10 12 7 5 6 6 0 0 0 0 0 0 101000000000 000001000000 164123000000 000001000000 220000000000 021010000000 100011000000 000100000000 100000000000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 33 25 23 NOT REMARKABLE: 39 35 32 24 28 29 26 34 41 33 24 23 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, BONE 000010000000 010100000000 433 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 33 25 23 NOT REMARKABLE: 39 35 32 24 28 29 26 34 41 33 24 23 --OSTEODYSTROPHY, FIBROUS --HYPERPLASIA, CARTILAGE --N-SARCOMA 041000000000 000000010010 000001000000 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 33 25 23 NOT REMARKABLE: 32 29 22 17 21 23 18 16 29 23 14 16 --HYPOCELLULAR, MARROW --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW --CONGESTION --N-FIBROSARCOMA --HYPERPLASIA, MEGAKARYOCYTIC, MARROW 242311032140 320010000001 4 4 6 4 5 4 7 12 8 7 5 5 023113141222 000000001010 100000000000 010000000001 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 39 40 33 24 29 30 26 35 41 35 24 23 NOT REMARKABLE: 30 29 20 19 18 23 17 21 27 24 14 16 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, MYELOID, MARROW --HYPOCELLULAR, MARROW --CONGESTION --HYPERPLASIA, ERYTHROID, MARROW --HYPERPLASIA, MEGAKARYOCYTIC, MARROW --N-CARCINOMA 220010000000 7 5 8 4 6 5 8 10 12 9 5 5 121010001030 000000001010 024123140322 010000000001 000010000000 434 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 39 40 33 24 29 30 26 35 41 35 24 23 NOT REMARKABLE: 30 29 20 19 18 23 17 21 27 24 14 16 --MYELOFIBROSIS 000001000000 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 24 23 NOT REMARKABLE: 37 37 32 24 28 29 26 35 41 35 24 23 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, CARTILAGINOUS --HYPERPLASIA, BONE --OSTEODYSTROPHY, FIBROUS --N-SARCOMA 000010000000 100100000000 100100000000 031000000000 000001000000 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 38 39 33 25 27 29 25 35 40 34 25 22 --HEMORRHAGE --AXONAL SWELLING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --NECROSIS / HEMORRHAGE, WHITE MATTER --DEMYELINATION --MINERALIZATION, DURA 000010100001 000000001000 100000000000 010001000000 000000000100 000010000000 THYMUS (TH) .......................................... NUMBER EXAMINED: 39 35 30 23 27 27 25 34 39 31 23 23 NOT REMARKABLE: 6 3 1 2 2 1 5 5 4 5 1 4 --HEMORRHAGE 201232112122 435 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYMUS (TH) .......................................... NUMBER EXAMINED: 39 35 30 23 27 27 25 34 39 31 23 23 NOT REMARKABLE: 6 3 1 2 2 1 5 5 4 5 1 4 --DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --M-THYMIC CARCINOMA --CYST --INFLAMMATION, CHRONIC-ACTIVE --EDEMA --HYPERPLASIA, LYMPHORETICULAR --INFLAMMATION, GRANULOMATOUS --HYPERPLASIA, EPITHELIAL 33 31 29 21 25 26 17 29 31 24 20 18 020000000100 000000001000 000010202030 000001000000 000000100000 000000001000 001000000000 000000000100 LUNG (LU) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 25 26 21 16 10 11 15 17 27 20 5 10 --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --MINERALIZATION, VASCULAR --N-CARCINOMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --INFLAMMATION, GRANULOMATOUS --HYALINE MATERIAL, ALVEOLAR SEPTA --HYPERPLASIA, TYPE II PNEUMOCYTE --LEUKOCYTES, INCREASED, INTRAVASCULAR 200310120210 230011001101 874287047645 000010020211 100211010100 2 3 7 1 9 9 9 8 6 6 16 8 002201220113 101002001100 001000000000 001000010100 010001000000 436 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 25 26 21 16 10 11 15 17 27 20 5 10 --MINERALIZATION, SEPTAL --INFLAMMATION, VASCULAR --EDEMA, ALVEOLAR --FIBROSIS --MINERALIZATION, ALVEOLAR LUMINA --INFLAMMATION, SUBACUTE --B-ADENOMA, BRONCHIOLAR-ALVEOLAR --N-SARCOMA --EMBOLISM, ADIPOSE TISSUE --INFILTRATE, MACROPHAGE, PIGMENTED 022100010010 000000010010 100000000010 000100000000 000100000000 010000000000 000010000000 101003000000 000000010000 000000000100 HEART (HT) ........................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 11 8 6 13 11 17 21 27 21 13 16 --CARDIOMYOPATHY --INFILTRATE, LYMPHOHISTIOCYTIC --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --MINERALIZATION, VASCULAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --INFLAMMATION, VASCULAR --M-ATRIOCAVAL MESOTHELIOMA --METAPLASIA, OSSEOUS / CARTILAGINOUS --N-SARCOMA 16 27 25 18 16 19 8 14 13 12 12 7 200100000000 100100000000 110000000000 142211000220 210010000010 130011000020 000001000001 010000000000 100010101000 101001000000 437 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** HEART (HT) ........................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 11 8 6 13 11 17 21 27 21 13 16 --INFLAMMATION, CHRONIC-ACTIVE 000000000100 MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 37 35 31 23 21 27 26 33 40 34 24 23 --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --BACTERIAL COLONIES --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --M-SARCOMA, UNDIFFERENTIATED 000100000000 000100000000 000100000000 141163010110 011020010000 000000001000 100000000000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 39 39 33 25 28 30 25 35 41 35 25 23 NOT REMARKABLE: 38 37 33 24 26 29 25 35 41 34 25 23 --DEGENERATION 120121000100 TRACHEA (TR) ......................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 39 40 33 24 29 30 26 34 41 35 25 23 --EXUDATE, LUMINAL --INFLAMMATION, CHRONIC-ACTIVE 000100010000 000000010000 438 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 38 39 33 22 29 30 25 35 41 35 24 23 --HYPERKERATOSIS --EXUDATE, LUMINAL --DILATATION, LUMINAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 000100100010 000200000000 100000000000 010000000000 THYROID (TY) ......................................... NUMBER EXAMINED: 39 39 33 25 28 29 26 35 41 35 25 23 NOT REMARKABLE: 23 23 23 17 18 20 10 22 24 23 18 10 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --HYPERTROPHY, FOLLICULAR CELL --INFLAMMATION, VASCULAR --M-CARCINOMA, C-CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 5 5 4 2 2 2 10 11 13 6 6 11 110010000000 421341000000 443042634712 332204000100 000110000000 000000100000 000001000000 010000000000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 38 38 33 23 28 27 26 35 40 34 25 23 NOT REMARKABLE: 33 32 28 21 26 26 26 33 40 34 25 23 --HYPERPLASIA --B-ADENOMA 565221010000 000000010000 439 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PITUITARY (PI) ....................................... NUMBER EXAMINED: 39 40 33 25 28 30 26 35 41 35 25 23 NOT REMARKABLE: 7 6 1 4 6 3 0 2 1 3 2 1 --B-ADENOMA --HYPERPLASIA --ANGIECTASIS --CYST --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, CHRONIC-ACTIVE --NECROSIS --M-CARCINOMA --I-SARCOMA 26 27 31 20 19 21 26 31 38 30 21 21 562134021121 100002011000 011013020000 000000001000 100000000000 100000000000 000000001100 010000000000 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 39 40 32 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 36 32 24 20 26 24 18 29 33 29 20 19 --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --DYSPLASIA, EPITHELIAL, GLANDULAR --INFLAMMATION, VASCULAR --I-SARCOMA 376526868643 002010000011 000000000010 000010000000 000000001000 010000000000 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 39 39 32 24 28 30 26 34 41 34 24 23 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, GLANDULAR 010000000010 000100000000 440 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 39 39 32 24 28 30 26 34 41 34 24 23 --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --M-SCHWANNOMA --UNILATERALLY EXAMINED --I-SARCOMA 000010000000 000000000100 000000010000 000000010000 001000000000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 39 39 33 25 28 30 26 35 41 35 25 23 NOT REMARKABLE: 32 28 24 15 22 22 25 32 38 32 20 22 --EDEMA --INFLAMMATION, ACUTE --HEMORRHAGE --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR --MINERALIZATION, GLANDULAR MUCOSA --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --MINERALIZATION, VASCULAR 110111120120 112411120120 000100000000 202142000131 370112011010 000002000000 455323001010 053222010110 010110000000 000000001000 010010000000 STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 29 34 28 19 24 21 24 31 35 32 22 20 --ACANTHOSIS / HYPERKERATOSIS 10 6 5 6 5 9 2 4 6 3 3 2 441 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 29 34 28 19 24 21 24 31 35 32 22 20 --EDEMA --INFLAMMATION, SUBACUTE --ULCERATION --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS 000010002101 100000002010 453324141220 965655243311 000000000100 DUODENUM (DU) ........................................ NUMBER EXAMINED: 36 39 32 24 26 30 26 35 39 33 24 23 NOT REMARKABLE: 34 39 32 24 26 28 26 34 39 32 24 23 --INFLAMMATION, VASCULAR --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --EROSION --B-LEIOMYOMA 100001000100 100001000000 000001000000 100000000000 000000010000 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 31 36 27 19 21 26 25 33 35 32 22 21 NOT REMARKABLE: 31 36 27 19 21 26 25 33 35 32 22 21 ILEUM (IL) ........................................... NUMBER EXAMINED: 30 35 27 21 21 24 26 32 37 29 21 21 NOT REMARKABLE: 29 34 26 21 21 24 26 32 37 28 21 21 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --NECROSIS 010000000100 100000000000 100000000000 442 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ILEUM (IL) ........................................... NUMBER EXAMINED: 30 35 27 21 21 24 26 32 37 29 21 21 NOT REMARKABLE: 29 34 26 21 21 24 26 32 37 28 21 21 --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS 001000000000 001000000000 CECUM (CE) ........................................... NUMBER EXAMINED: 27 33 26 20 22 23 25 33 37 31 22 21 NOT REMARKABLE: 27 31 26 20 20 23 24 33 36 30 22 21 --EROSION --HEMORRHAGE --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --BACTERIA --ULCERATION --B-LIPOMA 020010000000 010020000000 020010101000 000000000100 000010000000 000010000000 000010000000 COLON (CO) ........................................... NUMBER EXAMINED: 34 37 29 23 22 27 26 34 40 33 23 23 NOT REMARKABLE: 34 36 29 23 22 25 26 34 40 31 23 23 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 010002000100 000000000100 RECTUM (RE) .......................................... NUMBER EXAMINED: 37 40 31 25 26 29 26 35 41 35 25 23 NOT REMARKABLE: 35 39 31 25 26 27 24 35 38 35 22 22 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE 110002102021 000000000010 443 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** RECTUM (RE) .......................................... NUMBER EXAMINED: 37 40 31 25 26 29 26 35 41 35 25 23 NOT REMARKABLE: 35 39 31 25 26 27 24 35 38 35 22 22 --ULCERATION --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --NECROSIS 000000000010 000000100000 000000001000 100000000000 SKIN (SK) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 32 28 25 20 20 24 22 34 37 32 22 21 --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --CYST, EPIDERMAL INCLUSION --BACTERIAL COLONIES --SUPERFICIAL CRUSTING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --B-KERATOACANTHOMA --M-FIBROSARCOMA --B-FIBROMA --FIBROSIS --B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS --B-LIPOMA --M-CARCINOMA, SQUAMOUS CELL --B-RHABOMYOMA --B-PAPILLOMA, SQUAMOUS CELL 321150111111 350042101111 030020100101 100000000000 141000000000 100020000000 100010001010 110001001001 123324000000 212110101010 021010001000 010000100000 010000000000 010000000200 100000100000 000001000000 010000200000 444 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 32 28 25 20 20 24 22 34 37 32 22 21 --INFLAMMATION, CHRONIC --ANGIECTASIS WITH THROMBOSIS AND ENDOTHELIAL PROLIFERATION 000000010010 000010000000 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 26 35 40 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 2 3 1 3 5 3 --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --B-ADENOMA --INFLAMMATION, CHRONIC --FIBROSIS 0 0 0 0 0 0 6 11 14 10 8 3 0 0 0 0 0 0 19 19 29 22 15 15 000000442200 0 0 0 0 0 0 8 18 14 18 4 8 0 0 0 0 0 0 11 12 29 16 5 9 000000444532 000000002010 000000010000 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 38 38 32 23 26 30 26 35 41 35 25 23 NOT REMARKABLE: 34 33 23 16 21 27 24 33 40 35 24 22 --HEMORRHAGE --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL --HYPERPLASIA, TRANSITIONAL CELL --MINERALIZED MATERIAL, LUMEN --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE 011110000000 105100111000 221321000000 112232020010 100000000001 000020000000 133212000010 445 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 38 38 32 23 26 30 26 35 41 35 25 23 NOT REMARKABLE: 34 33 23 16 21 27 24 33 40 35 24 22 --INFLAMMATION, VASCULAR 000000100000 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 19 25 27 19 21 18 --DILATATION --B-POLYP, ENDOMETRIAL, STROMAL --CYST --INFLAMMATION, CHRONIC-ACTIVE --HYPERTROPHY, ENDOMETRIUM --B-LEIOMYOMA --HYPERPLASIA, CYSTIC, ENDOMETRIAL 000000446722 000000211300 000000002000 000000122001 000000000001 000000010000 000000264722 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 26 34 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 24 27 33 31 22 17 --HYPERPLASIA, MUCINOUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, STROMAL --M-SARCOMA, STROMAL --B-POLYP, STROMAL --EXUDATE, LUMINAL 000000154425 000000100000 000000002011 000000010000 000000011000 000000011000 446 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 26 34 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 16 19 19 15 14 11 --LUMEN, EXUDATE --HYPERPLASIA, MUCINOUS --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, CHRONIC-ACTIVE --B-POLYP, STROMAL 0 0 0 0 0 0 6 13 10 12 9 4 0 0 0 0 0 0 4 9 14 12 5 8 000000000100 000000100000 000000002000 000000001000 PROSTATE (PR) ........................................ NUMBER EXAMINED: 39 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 13 4 2 6 5 6 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --HYPERPLASIA, EPITHELIAL 25 35 30 19 24 23 0 0 0 0 0 0 111001000000 000010000000 100000000000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 38 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 32 28 20 15 22 22 0 0 0 0 0 0 --SECRETION, DECREASED --SECRETION, INCREASED --HYPERPLASIA, EPITHELIAL --INFLAMMATION, CHRONIC-ACTIVE --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --EDEMA 324122000000 047212000000 010000000000 222111000000 242632000000 000100000000 000001000000 447 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 39 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 32 36 23 18 19 20 0 0 0 0 0 0 --IMMATURE SPERM FORMS --INFILTRATE, LYMPHOHISTIOCYTIC --HYPOSPERMIA --ATROPHY/DEGENERATION --UNILATERALLY EXAMINED --VACUOLATION, TUBULAR EPITHELIUM --GRANULOMA, SPERM --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, EOSINOPHILIC --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 122162000000 103132000000 212202000000 000120000000 000002000000 122301000000 100000000000 100000000000 001000000000 100000000000 000001000000 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 36 37 33 25 27 29 25 35 39 34 24 22 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 120010001000 210011101111 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --UNDETERMINED MICROSCOPICALLY --NEOPLASM, BRAIN --NEOPLASM, HEMATOPOIETIC --NEOPLASM, PITUITARY 823332000112 221100000100 230021001101 18 21 20 14 12 15 18 24 33 24 15 16 448 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --NEOPLASM, SKIN/MUSCLE/SUBCUTIS --INFLAMMATION / INFECTION --NEOPLASM, MAMMARY GLAND --NEOPLASM, ABDOMINAL CAVITY --HEMORRHAGE --NEPHROPATHY --GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMATION --POSSIBLE TRAUMATIC INJURY --INFECTION / NECROSIS / CALCULUS, URINARY TRACT --NEOPLASM, PANCREAS --NEOPLASM, THORACIC CAVITY --HEPATIC ANOMALY / OBSTRUCTIVE HEPATOPATHY --HEMANGIOSARCOMA --NEOPLASM, HEART --DEGENERATION/NECROSIS/HEMORRHAGE, SPINAL CORD/BRAIN --NEOPLASM, LIVER --VASCULITIS, SYSTEMIC --NEOPLASM, UT/CV/VA/OV --NEOPLASM, KIDNEY --NEOPLASM, SPLEEN --EMBOLISM, PULMONARY --NEOPLASM, ADRENAL 323211101010 211142001011 000010585543 000120000000 000000000100 163212000210 100001000000 101000000000 100111000000 010000100010 000011000000 000000000010 000001000000 010000000000 010000000000 001000000000 000000100000 000000010000 000001000000 000002000000 000000010000 000010010000 449 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 2 2 0 3 2 2 0 2 1 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MAST CELL --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC --FIBROSIS --LYMPHANGIECTASIS 010010001000 012022200110 000000001010 010000000000 000000200000 001000000000 001000000000 001000000000 000001000000 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 1 4 4 1 3 2 7 14 11 13 3 7 NOT REMARKABLE: 1 0 2 0 1 1 2 4 0 4 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, RETICULOENDOTHELIAL --CONGESTION --FIBROPLASIA, PERIPHERAL 010000000000 011120121102 0 3 1 1 1 0 5 8 10 8 2 5 010001101313 000000011000 001001000000 000000000001 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 1 0 0 0 0 1 6 1 2 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 3 1 0 1 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 010000000000 450 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 1 0 0 0 0 1 6 1 2 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 3 1 0 1 0 --HYPERPLASIA, LYMPHOPLASMACYTIC 000000130201 LN, OTHER (LN) ....................................... NUMBER EXAMINED: 1 1 2 0 1 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 1 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 110000000000 001000000000 001010000010 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 3 3 2 1 2 0 1 0 1 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --DILATATION, SINUSOIDAL --N-SARCOMA --LYMPHANGIECTASIS 010000000000 022201000010 012111000100 001100010100 001100000000 000001000000 000001000000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 2 3 6 0 2 4 6 4 10 7 5 2 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, LYMPHOPLASMACYTIC 010000000000 116010649752 451 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 2 3 6 0 2 4 6 4 10 7 5 2 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE --LYMPHANGIECTASIS --INFILTRATE, MACROPHAGE, PIGMENTED --CONGESTION --I-SARCOMA --ABSCESS --INFLAMMATION, CHRONIC-ACTIVE 000012102111 001001000000 000000001001 012002000001 000001000000 000000010000 000000000001 VERTEBRA/MARROW (VT) ................................. NUMBER EXAMINED: 0 1 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) 010000000000 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 1 3 4 3 3 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 1 0 0 0 0 0 0 0 0 --HYPERPLASIA, CYSTIC --SECRETORY MATERIAL IN LUMINA --GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --M-CARCINOMA 001100000000 000100000000 134021000000 110000000000 001110000000 000002000000 452 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COAGULATING GL (CG) .................................. NUMBER EXAMINED: 1 0 0 1 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --SECRETORY MATERIAL, INCREASED 100100000000 TAIL (TI) ............................................ NUMBER EXAMINED: 2 0 4 1 0 0 1 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --CYST, EPIDERMAL INCLUSION --ACANTHOSIS / HYPERKERATOSIS --INFLAMMATION, VASCULAR --M-NEUROFIBROSARCOMA 204100011000 001000001000 100000000000 102100010000 000000100000 001000000000 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 1 4 2 2 4 1 3 7 4 0 2 NOT REMARKABLE: 0 0 0 0 1 1 0 0 0 3 0 0 --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, SUBACUTE --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE 002112001000 213112135002 011111002101 000000001000 001000000000 000000001000 000000000001 453 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 0 0 1 2 2 3 0 0 0 0 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION / NECROSIS, FAT --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --HEMATOMA --INFLAMMATION, VASCULAR --THROMBUS 001202000001 000010000000 000000000010 000010000000 000001000000 000001000000 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 1 2 0 0 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-CARCINOMA --I-SARCOMA 011000001000 001000000000 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 1 1 0 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 011010000000 001000000000 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 2 0 0 3 4 1 2 1 1 2 1 1 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --ACANTHOSIS / HYPERKERATOSIS 100341211211 100221111200 000131111211 454 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 31 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 2 0 0 3 4 1 2 1 1 2 1 1 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 0 0 --B-FIBROMA --FIBROSIS --BACTERIA 000000100000 000010001000 000010010000 AORTA (AO) ........................................... NUMBER EXAMINED: 0 1 3 2 1 1 0 0 0 1 0 0 NOT REMARKABLE: 0 0 1 0 0 1 0 0 0 0 0 0 --M-LIPOSARCOMA --MINERALIZATION 000010000000 012200000100 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 1 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --I-SARCOMA 011000000000 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 1 1 0 0 1 1 1 1 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 010000111010 001000110010 001001011000 455 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 32 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- HEAD, CORONAL (HC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE, EAR 000000000100 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 0 0 2 0 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --PROBABLE FRACTURE 002010000000 001000000000 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 1 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --ECTASIA, DUCTAL --INFLAMMATION, CHRONIC-ACTIVE --HYPERKERATOSIS 000000110000 000000010000 000000010000 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 1 0 0 --UNILATERALLY EXAMINED 000001000000 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 1 0 0 0 1 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 1 0 0 0 0 --M-LIPOSARCOMA --INFILTRATE, EOSINOPHILIC 000000100000 001000000000 456 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 33 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 1 0 0 0 1 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 1 0 0 0 0 --INFLAMMATION, GRANULOMATOUS 001000000000 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --I-LIPOSARCOMA 000000100000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --B-HIBERNOMA 000000100000 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 1 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --DILATATION, SINUSOIDAL --HEMORRHAGE --N-SARCOMA 000000001000 000001000000 000001000000 000001000000 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 1 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, PLASMACYTIC 000000001000 000000000001 457 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 34 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC 001000000000 001000000000 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 0 0 1 0 0 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA, UNDIFFERENTIATED --N-SARCOMA 000001000000 001000000000 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA 000001000000 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001000000000 AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001000000000 458 TABLE 39 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Unscheduled Deaths and Sacrifices PAGE: 35 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 000000000100 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 1 0 0 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --CYST --INFLAMMATION, CHRONIC --THROMBUS 001000000000 001000000000 001000000000 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 ** END OF LIST ** 459 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 65 44 38 30 65 30 65 40 46 40 65 24 NOT REMARKABLE: 58 41 33 29 60 28 60 35 42 35 59 22 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION --INFLAMMATION, ACUTE --I-NEUROFIBROSARCOMA --ATROPHY, RETINAL --DEGENERATION, LENTICULAR --INFLAMMATION, CHRONIC-ACTIVE --UNILATERALLY EXAMINED --ULCERATION, CORNEAL --DEGENERATION, RETINAL 613142412331 100010231231 001000000000 001000000100 010000010000 010000001010 000000001000 000000001010 000000100000 BRAIN (BR) ........................................... NUMBER EXAMINED: 65 45 45 35 65 34 65 48 51 48 65 30 NOT REMARKABLE: 42 24 14 10 43 17 32 15 13 15 37 10 --DILATATION, VENTRICULAR --M-OLIGODENDROGLIOMA --COMPRESSION, VENTRAL --HEMORRHAGE --M-ASTROCYTOMA --DEGENERATION, FOCAL --MINERALIZATION --INFILTRATE, MACROPHAGE, PIGMENTED --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --M-GRANULAR CELL TUMOR 13 12 14 10 8 7 16 14 20 11 14 11 100000000000 20 15 26 23 18 16 30 33 38 28 27 19 201110411101 200100000100 001000000000 014120101210 120000110201 000000010000 100010000000 010000000000 460 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** BRAIN (BR) ........................................... NUMBER EXAMINED: 65 45 45 35 65 34 65 48 51 48 65 30 NOT REMARKABLE: 42 24 14 10 43 17 32 15 13 15 37 10 --I-CARCINOMA --M-MENINGEAL SARCOMA --M-NEUROFIBROSARCOMA 000000101201 010000000000 001000000000 LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 0 0 0 0 1 6 1 1 1 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL 51 43 44 45 45 31 42 42 38 41 56 32 0 2 4 22 42 3 2 1 4 16 52 2 0 0 0 0 14 0 0 0 0 7 36 1 0 0 0 0 6 0 0 0 0 1 36 0 5 4 6 5 14 4 7 6 6 6 15 3 3 3 6 13 19 3 1 1 0 1 4 1 15 14 14 16 15 9 15 15 22 16 22 11 20 21 23 24 24 16 27 21 18 17 21 13 10 7 16 17 13 8 16 20 13 21 20 11 5 15 19 17 22 15 0 1 1 2 4 1 141624324222 111110221000 220031001101 4 3 8 3 7 5 10 17 15 8 7 5 20 20 25 24 25 21 23 25 19 17 30 17 8 7 11 7 14 4 9 13 4 9 10 4 4 1 7 2 2 5 12 10 9 4 3 7 461 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 0 0 0 0 1 6 1 1 1 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED --VACUOLATION, HEPATOCELLULAR --DEGENERATION / NECROSIS, MIDZONAL --DEGENERATION / NECROSIS, CENTRILOBULAR --HYPERTROPHY, HEPATOCELLULAR --HEMORRHAGE --CYST, BILIARY --INFLAMMATION, SUBACUTE --GLYCOGEN, HEPATOCELLULAR, INCREASED --ANOMALY, SECONDARY TO DIAPHRAGMATIC HERNIA --ANGIECTASIS --LEUKOCYTOSIS, SINUSOIDAL --B-ADENOMA, HEPATOCELLULAR --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, BILE DUCT --STASIS, BILE --MITOSIS, HEPATOCELLULAR, INCREASED --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --N-SARCOMA --B-CHOLANGIOMA --N-CARCINOMA --KARYOMEGALY --M-CARCINOMA, HEPATOCELLULAR 6 5 3 4 5 2 2 3 5 6 23 7 623173320332 000010000000 100151010221 000010000000 001000010100 010101311142 010000200010 130010000000 001000000001 112020612112 000010010000 033170011152 000111000110 000000000010 000000000010 000010011000 003100110210 000001000000 000000000100 000110000000 010000000000 000000000010 462 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LIVER-OIL RED O (LI0) ................................ NUMBER EXAMINED: 5 0 0 0 5 0 5 0 0 0 5 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR 500050500050 KIDNEY (KD) .......................................... NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 4 2 4 2 3 4 12 7 5 8 1 --NEPHROPATHY, CHRONIC PROGRESSIVE --INFILTRATE, LYMPHOHISTIOCYTIC --DILATATION, PELVIC --MINERALIZATION, PELVIC --REGENERATION, TUBULAR EPITHELIUM --MINERALIZATION, TUBULAR --HEMATOPOIESIS, EXTRAMEDULLARY --ATROPHY, TUBULAR --VACUOLATION, TUBULAR EPITHELIUM --N-CARCINOMA --PIGMENT, TUBULAR --KARYOMEGALY, TUBULAR CELL --HYALINE DROPLETS, TUBULAR EPITHELIUM --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --INFLAMMATION, CHRONIC-ACTIVE, PELVIC --NECROSIS, PAPILLARY --ECTASIA, TUBULAR --CAST, PROTEINACEOUS 49 43 49 45 50 36 35 29 26 28 40 21 522340314220 3 5 4 2 10 8 2 2 4 1 3 2 12 7 10 10 7 5 47 34 36 42 36 31 321421110000 2 0 0 1 4 1 14 8 11 7 19 6 010000011100 000000001000 000020110210 000000000001 310030102141 000000010000 510140000000 201000100010 1 2 3 5 1 3 6 10 8 7 5 5 123302135222 133111010021 000020000000 110100000010 000000000010 463 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................................... NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 4 2 4 2 3 4 12 7 5 8 1 --CYST --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS, CORTICAL --ANGIECTASIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --BACTERIAL COLONIES --DEGENERATION, CYSTIC, UNILATERAL --INFILTRATE, FAT, INTERSTITIAL --N-SARCOMA --M-CARCINOMA, TUBULAR CELL --ULCERATION, TRANSITIONAL EPITHELIUM, PELVIC --M-LIPOSARCOMA --THROMBUS --B-LIPOMA --THROMBUS, GLOMERULAR --B-PAPILLOMA, TRANSITIONAL CELL --M-RENAL MESENCHYMAL TUMOR --DEGENERATION TUBULAR --INFARCT 030202111000 100110100000 100000000000 000001000000 100000000000 000100000000 000000000100 000000001000 100000000000 100000100000 000001000000 000001000000 001210000100 000010101000 000010000000 000001000000 001000000000 000010100000 000000100000 SPLEEN (SP) .......................................... NUMBER EXAMINED: 65 45 38 30 65 31 65 40 46 41 65 23 NOT REMARKABLE: 9 7 6 8 14 6 2 0 1 0 4 0 --PIGMENT, INCREASED --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED 39 29 23 16 30 14 48 23 35 28 51 14 24 16 15 9 27 13 39 23 16 16 23 15 464 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SPLEEN (SP) .......................................... NUMBER EXAMINED: 65 45 38 30 65 31 65 40 46 41 65 23 NOT REMARKABLE: 9 7 6 8 14 6 2 0 1 0 4 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --DEPLETION, LYMPHOCYTIC --FIBROSIS, CAPSULAR --CYST, SEROSAL --M-OSTEOSARCOMA --HYPERPLASIA, LYMPHORETICULAR --M-HEMANGIOSARCOMA --HEMORRHAGE --NECROSIS 110020000100 000010001001 100001101010 000000000010 000001000000 000000000101 000001000000 000000010000 000000010000 PANCREAS (PA) ........................................ NUMBER EXAMINED: 65 54 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 21 14 12 12 17 8 43 32 32 31 40 21 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --NECROSIS / INFLAMMATION, ADIPOSE TISSUE --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --B-ADENOMA, ACINAR CELL --VACUOLATION, FOCAL --INFILTRATION, FAT, INTERSTITIAL 11 15 11 14 16 8 3 11 14 221411302 24 17 18 13 19 13 1 2 1 323222210 778973235 000000100 230133152 122553101 434441221 010000000 000000010 11 16 20 14 12 6 10 7 4 9 12 10 331 111 000 243 000 101 010 020 000 000 967 465 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ........................................ NUMBER EXAMINED: 65 54 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 21 14 12 12 17 8 43 32 32 31 40 21 --INFLAMMATION, VASCULAR --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFILTRATE, EOSINOPHILIC --FIBROSIS --INFLAMMATION, GRANULOMATOUS --NECROSIS --ECTOPIC HEPATOCYTES --B-FIBROMA --INFLAMMATION, CHRONIC-ACTIVE 214414011001 100002000000 000001001000 001000000000 001000000110 001000000000 000010000000 000100000000 000000000010 000000001010 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 65 47 38 31 65 30 65 45 47 45 65 26 NOT REMARKABLE: 18 8 13 12 18 8 6 7 8 6 8 1 --VACUOLATION, FOCAL --UNILATERALLY EXAMINED --CONGESTION --ANGIECTASIS --HEMATOPOIESIS, EXTRAMEDULLARY --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --VACUOLATION, DIFFUSE --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL 9 15 10 110 100 333 101 600 000 10 5 3 121 232 422 3 15 00 00 11 00 04 00 2 16 11 02 24 7805333 1210200 0100100 2 21 16 24 15 13 9 3254510 0 5 0 1 0 11 0 0110200 7 26 17 11 16 27 12 0000000 0 25 16 9 17 20 10 1443552 466 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 65 47 38 31 65 30 65 45 47 45 65 26 NOT REMARKABLE: 18 8 13 12 18 8 6 7 8 6 8 1 --PIGMENT --MINERALIZATION --ATROPHY, CORTICAL --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --DEGENERATION / NECROSIS, UNILATERAL --NECROSIS, FOCAL --FIBROSIS --N-SARCOMA --M-CARCINOMA --CORTICAL TISSUE, EXTRACAPSULAR 23 26 16 15 26 14 36 28 28 25 40 16 100001000000 011110010100 301210111220 100010000000 001010011011 001000000000 001001000010 000001000000 000010010001 010000000000 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 65 47 38 31 65 30 65 45 47 45 65 24 NOT REMARKABLE: 57 33 27 26 52 26 54 33 42 33 54 22 --UNILATERALLY EXAMINED --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 331121643760 358162120212 365351552350 120010010000 LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 64 43 38 30 65 30 65 43 46 40 65 24 NOT REMARKABLE: 60 38 36 26 58 29 64 39 44 36 62 22 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 310120110121 010020000100 467 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 64 43 38 30 65 30 65 43 46 40 65 24 NOT REMARKABLE: 60 38 36 26 58 29 64 39 44 36 62 22 --M-HEMANGIOSARCOMA --HYPERPLASIA, LYMPHOPLASMACYTIC --MINERALIZATION, VASCULAR --HYPERPLASIA, RETICULOENDOTHELIAL --HYPERPLASIA, LYMPHORETICULAR --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, PIGMENTED --B-HEMANGIOMA --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS --ANGIECTASIS --INFLAMMATION, VASCULAR --LYMPHANGIECTASIS 000100000000 000111010110 131110000000 000000001000 000000001000 001000000000 001000000000 000000120001 000000000100 000010000000 000010000000 000010000000 000100000000 000000000010 OVARY (OV) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 65 42 48 46 65 31 NOT REMARKABLE: 0 0 0 0 0 0 51 36 40 34 47 19 --CYST --HEMATOMA --B-GRANULOSA/THECA CELL TUMOR --HYPERPLASIA, GRANULOSA CELL --CORPORA LUTEA, PERSISTENT --UNILATERALLY EXAMINED 0 0 0 0 0 0 12 6 7 12 13 8 000000000010 000000100000 000000200063 000000000001 000000001020 468 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- TESTIS (TE) .......................................... NUMBER EXAMINED: 65 55 55 55 65 39 0 0 0 0 0 0 NOT REMARKABLE: 49 32 38 41 49 27 0 0 0 0 0 0 --ATROPHY/DEGENERATION --GRANULOMA, SPERM --UNILATERALLY EXAMINED --INFLAMMATION, VASCULAR --M-HEMANGIOSARCOMA --MINERALIZATION, VASCULAR --HYPERPLASIA, INTERSTITIAL CELL (LEYDIG --B-INTERSTITIAL CELL TUMOR --M-MALIGNANT INTERSTITIAL CELL TUMOR --INFILTRATE, LYMPHOHISTIOCYTIC CELL) 15 16 12 9 11 6 0 0 0 0 0 0 201000000000 000001000000 185423000000 000001000000 230110000000 022021000000 112211000000 000100000000 101010000000 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 33 60 23 NOT REMARKABLE: 60 35 32 24 59 29 60 34 41 33 59 23 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, BONE --OSTEODYSTROPHY, FIBROUS --HYPERPLASIA, CARTILAGE --N-SARCOMA 000010000000 010100000000 041000000000 000000010010 000001000000 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 33 60 23 NOT REMARKABLE: 48 29 22 17 48 23 42 16 29 23 40 16 --HYPOCELLULAR, MARROW --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 742351532170 320010000001 469 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 33 60 23 NOT REMARKABLE: 48 29 22 17 48 23 42 16 29 23 40 16 --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW --CONGESTION --N-FIBROSARCOMA --HYPERPLASIA, MEGAKARYOCYTIC, MARROW 4 4 6 4 5 4 12 12 8 7 10 5 023113141232 000000001010 100000000000 010000000001 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 60 40 33 24 60 30 60 35 41 35 59 23 NOT REMARKABLE: 50 29 20 19 49 23 46 21 27 24 44 16 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, MYELOID, MARROW --HYPOCELLULAR, MARROW --CONGESTION --HYPERPLASIA, ERYTHROID, MARROW --HYPERPLASIA, MEGAKARYOCYTIC, MARROW --N-CARCINOMA --MYELOFIBROSIS 220010000000 7 5 8 4 6 5 13 10 12 9 9 5 221010001030 000000001010 024123140332 010000000001 000010000000 000001000000 BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 59 23 NOT REMARKABLE: 58 37 32 24 58 29 59 35 41 35 58 23 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, CARTILAGINOUS --HYPERPLASIA, BONE --OSTEODYSTROPHY, FIBROUS 000020000000 100100100010 100100000000 031000000000 470 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 59 23 NOT REMARKABLE: 58 37 32 24 58 29 59 35 41 35 58 23 --N-SARCOMA 000001000000 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 59 39 33 25 58 29 58 35 40 34 60 22 --HEMORRHAGE --AXONAL SWELLING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --NECROSIS / HEMORRHAGE, WHITE MATTER --DEMYELINATION --MINERALIZATION, DURA --DEGENERATION, AXONAL --INFILTRATE, LYMPHOHISTIOCYTIC 000010100001 000000001000 100000000000 010001000000 000000000100 000010000000 000000100000 000000100000 THYMUS (TH) .......................................... NUMBER EXAMINED: 55 35 30 24 54 27 57 34 39 31 57 25 NOT REMARKABLE: 13 3 1 2 5 1 16 5 4 5 12 4 --HEMORRHAGE --DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --M-THYMIC CARCINOMA --CYST --INFLAMMATION, CHRONIC-ACTIVE --EDEMA 401262312142 42 31 29 22 46 26 35 29 31 24 40 18 020000000100 000010001000 000010202041 000001000000 000000100000 471 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYMUS (TH) .......................................... NUMBER EXAMINED: 55 35 30 24 54 27 57 34 39 31 57 25 NOT REMARKABLE: 13 3 1 2 5 1 16 5 4 5 12 4 --HYPERPLASIA, LYMPHORETICULAR --INFLAMMATION, GRANULOMATOUS --HYPERPLASIA, EPITHELIAL 000000101001 001000000000 000000000101 LUNG (LU) ............................................ NUMBER EXAMINED: 60 50 50 49 60 40 60 50 50 50 60 40 NOT REMARKABLE: 33 33 31 27 25 14 34 25 31 28 17 19 --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --MINERALIZATION, VASCULAR --N-CARCINOMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --INFLAMMATION, GRANULOMATOUS --HYALINE MATERIAL, ALVEOLAR SEPTA --HYPERPLASIA, TYPE II PNEUMOCYTE --LEUKOCYTES, INCREASED, INTRAVASCULAR --MINERALIZATION, SEPTAL --INFLAMMATION, VASCULAR --EDEMA, ALVEOLAR --FIBROSIS --MINERALIZATION, ALVEOLAR LUMINA --INFLAMMATION, SUBACUTE --B-ADENOMA, BRONCHIOLAR-ALVEOLAR 300310120210 230021001101 15 8 9 12 18 10 4 5 8 11 11 7 000110130222 200231210110 6 5 8 2 16 14 17 12 11 10 32 11 304222730384 101002001100 001000000000 103020020210 010001000000 022100010010 000000010010 100000000010 000100000000 000100000001 010000000000 000010000000 472 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................................ NUMBER EXAMINED: 60 50 50 49 60 40 60 50 50 50 60 40 NOT REMARKABLE: 33 33 31 27 25 14 34 25 31 28 17 19 --N-SARCOMA --EMBOLISM, ADIPOSE TISSUE --INFILTRATE, MACROPHAGE, PIGMENTED --FIBROSIS, PLEURAL/SUBPLEURAL 101103000000 000000010000 000000000101 000000000010 HEART (HT) ........................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 25 11 8 6 19 11 37 21 27 21 34 16 --CARDIOMYOPATHY --INFILTRATE, LYMPHOHISTIOCYTIC --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --MINERALIZATION, VASCULAR --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --INFLAMMATION, VASCULAR --M-ATRIOCAVAL MESOTHELIOMA --METAPLASIA, OSSEOUS / CARTILAGINOUS --N-SARCOMA --INFLAMMATION, CHRONIC-ACTIVE --M-ENDOCARDIAL SCHWANNOMA 30 27 25 18 41 19 22 14 13 12 25 7 200100000000 100100000000 110000000000 142221000220 000010000000 210010000010 130011000020 000001000001 010000000000 100010101000 101001000000 000000000100 000000000010 473 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 36 60 23 NOT REMARKABLE: 57 35 31 23 50 27 60 33 40 35 59 23 --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --BACTERIAL COLONIES --DEGENERATION --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --M-SARCOMA, UNDIFFERENTIATED 000100000000 000100000000 000100000000 000010000000 141173010110 011020010000 000000001000 200000000000 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 60 39 33 25 59 30 59 35 41 35 60 23 NOT REMARKABLE: 59 37 33 24 56 29 59 35 41 34 60 23 --DEGENERATION 120131000100 TRACHEA (TR) ......................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 60 40 33 24 59 30 60 34 41 35 60 23 --EXUDATE, LUMINAL --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 000100010000 000000010000 000010000000 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 60 40 33 25 60 30 59 35 41 35 60 23 NOT REMARKABLE: 59 39 33 22 59 30 58 35 41 35 59 23 --HYPERKERATOSIS 000100100010 474 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 60 40 33 25 60 30 59 35 41 35 60 23 NOT REMARKABLE: 59 39 33 22 59 30 58 35 41 35 59 23 --EXUDATE, LUMINAL --DILATATION, LUMINAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 000200000000 100000000000 010010000000 THYROID (TY) ......................................... NUMBER EXAMINED: 60 49 50 49 59 39 60 50 50 50 60 40 NOT REMARKABLE: 35 26 28 22 33 22 30 26 24 27 37 17 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --HYPERTROPHY, FOLLICULAR CELL --INFLAMMATION, VASCULAR --M-CARCINOMA, C-CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --B-HIBERNOMA --COLLOID, DECREASED 9 8 10 7 9 4 20 19 19 15 19 16 311211000100 633982000000 5 6 6 9 7 4 12 6 6 8 5 6 354449000211 001110000000 000000100000 001001010001 010010000000 000000000010 000000001000 PARATHYROID (PT) ..................................... NUMBER EXAMINED: 59 48 50 47 59 37 60 49 49 49 59 39 NOT REMARKABLE: 52 41 44 40 55 34 60 47 49 48 58 39 --HYPERPLASIA --B-ADENOMA 675643010100 101100010010 475 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PITUITARY (PI) ....................................... NUMBER EXAMINED: 60 45 46 36 59 38 60 50 47 46 60 34 NOT REMARKABLE: 13 6 1 4 16 3 6 2 1 3 11 1 --B-ADENOMA --HYPERPLASIA --ANGIECTASIS --CYST --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, CHRONIC-ACTIVE --NECROSIS --M-CARCINOMA --I-SARCOMA 37 29 42 31 37 28 46 41 42 37 40 30 10 9 6 3 10 5 11 8 3 5 12 4 200002322050 012113020121 000000001000 100000000000 100000000000 000000001201 010000000000 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 55 32 25 20 54 24 48 29 33 29 51 19 --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --DYSPLASIA, EPITHELIAL, GLANDULAR --INFLAMMATION, VASCULAR --I-SARCOMA 5 7 6 5 5 6 12 6 8 6 8 3 002010000011 000000000010 000010000000 000000001000 010000000000 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 60 39 32 24 59 30 60 34 41 34 59 23 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, GLANDULAR 010000000010 000100000000 476 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 60 39 32 24 59 30 60 34 41 34 59 23 --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --M-SCHWANNOMA --UNILATERALLY EXAMINED --I-SARCOMA 000010000000 000000000100 000000010000 000000010000 001000000000 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 60 45 39 30 59 34 60 37 42 35 60 27 NOT REMARKABLE: 48 29 24 18 43 23 56 34 38 32 53 24 --EDEMA --INFLAMMATION, ACUTE --HEMORRHAGE --CONGESTION --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR --MINERALIZATION, GLANDULAR MUCOSA --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --MINERALIZATION, VASCULAR 120111220120 223411421130 000100000000 000010000000 202372001142 571223011021 001003000010 8 9 11 3 8 6 0 0 1 0 1 0 053232010110 010120000000 000000001000 010010000000 477 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 60 40 33 25 60 30 60 36 41 35 60 23 NOT REMARKABLE: 49 34 28 19 55 21 58 31 35 32 56 20 --ACANTHOSIS / HYPERKERATOSIS --EDEMA --INFLAMMATION, SUBACUTE --ULCERATION --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS 11 6 5 6 5 9 2 5 6 3 4 2 000010002101 100000002010 453324141230 965655253321 000000000100 DUODENUM (DU) ........................................ NUMBER EXAMINED: 57 39 32 24 57 30 60 35 39 33 59 23 NOT REMARKABLE: 54 39 32 24 57 28 60 34 39 32 59 23 --INFLAMMATION, VASCULAR --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --EROSION --B-LEIOMYOMA 100001000100 200001000000 000001000000 100000000000 000000010000 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 52 36 27 19 52 26 59 33 35 32 57 22 NOT REMARKABLE: 52 36 27 19 52 26 59 33 35 32 57 21 --M-CARCINOMA 000000000001 ILEUM (IL) ........................................... NUMBER EXAMINED: 51 35 27 21 52 24 60 32 37 29 56 21 NOT REMARKABLE: 49 34 26 21 52 24 59 32 37 28 54 21 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 010000000100 478 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ILEUM (IL) ........................................... NUMBER EXAMINED: 51 35 27 21 52 24 60 32 37 29 56 21 NOT REMARKABLE: 49 34 26 21 52 24 59 32 37 28 54 21 --HEMORRHAGE --NECROSIS --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS --INFLAMMATION, CHRONIC-ACTIVE --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 100000000000 100000000000 001000000000 001000000000 100000000010 000000100010 CECUM (CE) ........................................... NUMBER EXAMINED: 48 33 26 20 53 24 59 33 37 31 57 21 NOT REMARKABLE: 47 31 26 20 51 24 58 33 36 30 56 21 --EROSION --HEMORRHAGE --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --BACTERIA --ULCERATION --B-LIPOMA 020010000000 010020000000 120010101010 000000000100 000010000000 000010000000 000010000000 COLON (CO) ........................................... NUMBER EXAMINED: 55 37 29 23 53 27 60 35 40 33 58 23 NOT REMARKABLE: 52 36 29 23 53 25 59 34 40 31 57 23 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, CHRONIC-ACTIVE --DIVERTICULUM 210002000100 000000000100 100000000010 000000100000 479 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** COLON (CO) ........................................... NUMBER EXAMINED: 55 37 29 23 53 27 60 35 40 33 58 23 NOT REMARKABLE: 52 36 29 23 53 25 59 34 40 31 57 23 --B-LEIOMYOMA 000000010000 RECTUM (RE) .......................................... NUMBER EXAMINED: 58 40 31 25 57 29 59 35 41 35 60 23 NOT REMARKABLE: 54 39 31 25 54 27 57 35 38 35 52 22 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --NECROSIS 210032102061 100000000020 000000000010 000000100000 000000001000 100000000000 SKIN (SK) ............................................ NUMBER EXAMINED: 60 43 37 31 60 33 60 36 41 35 60 25 NOT REMARKABLE: 52 28 25 20 44 24 53 34 37 32 53 21 --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --DEGENERATION, MUSCLE, SUBCUTANEOUS --CYST, EPIDERMAL INCLUSION --BACTERIAL COLONIES --SUPERFICIAL CRUSTING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --B-KERATOACANTHOMA 421250311121 460163311122 030120300111 100000110000 000000100000 241020000010 100020000000 200020001010 110011001001 144656000001 480 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ............................................ NUMBER EXAMINED: 60 43 37 31 60 33 60 36 41 35 60 25 NOT REMARKABLE: 52 28 25 20 44 24 53 34 37 32 53 21 --M-FIBROSARCOMA --B-FIBROMA --FIBROSIS --B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS --B-LIPOMA --M-CARCINOMA, SQUAMOUS CELL --B-RHABOMYOMA --B-PAPILLOMA, SQUAMOUS CELL --INFLAMMATION, CHRONIC --ANGIECTASIS WITH THROMBOSIS AND ENDOTHELIAL --EDEMA PROLIFERATION 212210101010 033110001010 020110100000 011000100000 010010010210 110000100000 000001000000 010001200001 000000010010 000010000000 000010000000 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 60 50 49 50 60 40 NOT REMARKABLE: 0 0 0 0 0 0 5 4 2 3 8 3 --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --SECRETORY MATERIAL --M-FIBROSARCOMA --B-ADENOMA --INFLAMMATION, CHRONIC --FIBROSIS 0 0 0 0 0 0 11 12 15 11 14 4 0 0 0 0 0 0 35 28 32 28 28 22 000000642202 0 0 0 0 0 0 20 27 19 24 11 15 0 0 0 0 0 0 34 22 35 28 30 20 000000600050 000000100110 000000765744 000000002010 000000110000 481 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 59 48 49 48 57 40 60 50 50 50 59 40 NOT REMARKABLE: 51 38 36 33 45 36 57 44 49 50 55 37 --HEMORRHAGE --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL --HYPERPLASIA, TRANSITIONAL CELL --MINERALIZED MATERIAL, LUMEN --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE --INFLAMMATION, VASCULAR --DIVERTICULUM 011110000001 436501151032 344781000000 113542020011 100000000001 000020000000 133312000010 000000100000 000000100000 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 60 39 44 41 60 29 NOT REMARKABLE: 0 0 0 0 0 0 43 25 28 19 41 19 --DILATATION --B-POLYP, ENDOMETRIAL, STROMAL --CYST --INFLAMMATION, CHRONIC-ACTIVE --HYPERTROPHY, ENDOMETRIUM --B-LEIOMYOMA --HYPERPLASIA, CYSTIC, ENDOMETRIAL --M-LEIOMYOSARCOMA --HEMORRHAGE --B-ADENOMA 000000856974 000000221331 000000002000 000000122001 000000000001 000000010000 0 0 0 0 0 0 8 8 6 10 10 5 000000000001 000000000100 000000000100 482 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CERVIX (CV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 60 34 43 37 60 24 NOT REMARKABLE: 0 0 0 0 0 0 54 27 33 31 53 17 --CYST, SQUAMOUS --HYPERPLASIA, MUCINOUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, STROMAL --M-SARCOMA, STROMAL --B-POLYP, STROMAL --EXUDATE, LUMINAL --HYPERPLASIA, CYSTIC --HEMORRHAGE 000000100000 000000455545 000000100000 000000003011 000000010020 000000011001 000000011000 000000000100 000000000010 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 0 0 0 0 60 34 42 36 59 23 NOT REMARKABLE: 0 0 0 0 0 0 35 19 19 15 36 11 --LUMEN, EXUDATE --HYPERPLASIA, MUCINOUS --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, CHRONIC-ACTIVE --B-POLYP, STROMAL --I-CARCINOMA 0 0 0 0 0 0 10 13 10 12 13 4 0 0 0 0 0 0 15 9 15 13 13 8 000000000100 000000100000 000000002000 000000001000 000000100000 PROSTATE (PR) ........................................ NUMBER EXAMINED: 60 41 38 29 60 31 0 0 0 0 0 0 NOT REMARKABLE: 19 4 2 6 16 6 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE 39 36 35 23 44 24 0 0 0 0 0 0 483 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PROSTATE (PR) ........................................ NUMBER EXAMINED: 60 41 38 29 60 31 0 0 0 0 0 0 NOT REMARKABLE: 19 4 2 6 16 6 0 0 0 0 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --HYPERPLASIA, EPITHELIAL 211001000000 000010000000 100100000000 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 59 40 34 26 60 31 0 0 0 0 0 0 NOT REMARKABLE: 50 28 21 15 49 22 0 0 0 0 0 0 --SECRETION, DECREASED --SECRETION, INCREASED --HYPERPLASIA, EPITHELIAL --INFLAMMATION, CHRONIC-ACTIVE --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --EDEMA 524123000000 147222000000 010000000000 222241000000 242632000000 000100000000 000001000000 EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 60 40 33 25 60 30 0 0 0 0 0 0 NOT REMARKABLE: 50 36 23 18 45 20 0 0 0 0 0 0 --IMMATURE SPERM FORMS --INFILTRATE, LYMPHOHISTIOCYTIC --HYPOSPERMIA --ATROPHY/DEGENERATION --UNILATERALLY EXAMINED --VACUOLATION, TUBULAR EPITHELIUM --GRANULOMA, SPERM 122172000000 403142000000 212232000000 000120000000 000002000000 122311000000 100000000000 484 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 60 40 33 25 60 30 0 0 0 0 0 0 NOT REMARKABLE: 50 36 23 18 45 20 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, EOSINOPHILIC --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 100000000000 001000000000 100000000000 000001000000 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 57 37 33 25 56 29 59 35 39 34 59 22 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 120020001000 210021101111 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --UNDETERMINED MICROSCOPICALLY --NEOPLASM, BRAIN --NEOPLASM, HEMATOPOIETIC --NEOPLASM, PITUITARY --NEOPLASM, SKIN/MUSCLE/SUBCUTIS --INFLAMMATION / INFECTION --NEOPLASM, MAMMARY GLAND --NEOPLASM, ABDOMINAL CAVITY --HEMORRHAGE --NEPHROPATHY 26 15 22 30 36 10 39 20 14 20 40 17 823332000112 221100000100 230021001101 18 21 20 14 12 15 18 24 33 24 15 16 323211101010 211142001011 000010585543 000120000000 000000000100 163212000210 485 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMATION --POSSIBLE TRAUMATIC INJURY --INFECTION / NECROSIS / CALCULUS, URINARY TRACT --NEOPLASM, PANCREAS --NEOPLASM, THORACIC CAVITY --HEPATIC ANOMALY / OBSTRUCTIVE HEPATOPATHY --HEMANGIOSARCOMA --NEOPLASM, HEART --DEGENERATION/NECROSIS/HEMORRHAGE, SPINAL CORD/BRAIN --NEOPLASM, LIVER --VASCULITIS, SYSTEMIC --NEOPLASM, UT/CV/VA/OV --NEOPLASM, KIDNEY --NEOPLASM, SPLEEN --EMBOLISM, PULMONARY --NEOPLASM, ADRENAL 100001000000 101000000000 100111000000 010000100010 000011000000 000000000010 000001000000 010000000000 010000000000 001000000000 000000100000 000000010000 000001000000 000002000000 000000010000 000010010000 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 2 2 2 3 4 3 0 2 1 3 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MAST CELL --INFILTRATE, MACROPHAGE, PIGMENTED 010010001000 012022200120 000101101021 010000000000 000000200000 486 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 0 2 2 2 3 4 3 0 2 1 3 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC --FIBROSIS --LYMPHANGIECTASIS 001000000000 001000000000 001000000000 000202000010 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 5 6 6 4 9 3 14 18 13 18 9 11 NOT REMARKABLE: 4 0 2 2 5 2 5 4 1 5 2 2 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, RETICULOENDOTHELIAL --CONGESTION --FIBROPLASIA, PERIPHERAL 010010000000 012230321212 0 5 3 2 2 0 7 12 11 12 6 7 110101221413 000000011000 001001000000 000000000001 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 2 0 0 0 0 5 7 1 2 2 1 NOT REMARKABLE: 0 1 0 0 0 0 2 3 1 0 1 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --N-CARCINOMA 010000000000 000000140201 000000100010 000000100010 000000100000 487 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, OTHER (LN) ....................................... NUMBER EXAMINED: 1 1 2 0 1 0 0 0 0 0 2 0 NOT REMARKABLE: 0 0 1 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 110000000000 001000000010 001010000020 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 0 4 3 3 3 3 2 1 0 1 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --DILATATION, SINUSOIDAL --N-SARCOMA --LYMPHANGIECTASIS 010000000000 022301100010 022131100100 001101110100 001120000000 000001000000 000002000000 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 5 4 7 2 4 5 13 6 10 10 9 7 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 2 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --LYMPHANGIECTASIS --INFILTRATE, MACROPHAGE, PIGMENTED --CONGESTION --I-SARCOMA 010000000000 3 2 7 1 3 1 11 5 9 9 6 5 200133812225 101002000000 000010001001 012002000001 000001000000 488 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 5 4 7 2 4 5 13 6 10 10 9 7 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 2 0 --ABSCESS --INFLAMMATION, CHRONIC-ACTIVE 000000010000 000000000001 VERTEBRA/MARROW (VT) ................................. NUMBER EXAMINED: 0 1 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) 010000000000 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 3 4 5 4 5 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 1 0 0 0 0 0 0 0 0 --HYPERPLASIA, CYSTIC --SECRETORY MATERIAL IN LUMINA --GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --M-CARCINOMA --B-ADENOMA 001100000000 000100000000 245031000000 310000000000 001220000000 100002000000 000010000000 COAGULATING GL (CG) .................................. NUMBER EXAMINED: 2 0 0 1 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --SECRETORY MATERIAL, INCREASED --INFLAMMATION, CHRONIC-ACTIVE 100100000000 100000000000 489 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 31 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 5 0 4 2 0 2 1 1 1 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --CYST, EPIDERMAL INCLUSION --ACANTHOSIS / HYPERKERATOSIS --INFLAMMATION, VASCULAR --M-NEUROFIBROSARCOMA --SUPERFICIAL CRUSTING --B-KERATOACANTHOMA --M-LIPOSARCOMA 204102011100 001000001000 300000000000 202102010000 000000100000 001000000000 000001000000 000100000000 000000000100 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 4 1 5 6 2 6 2 4 9 8 7 6 NOT REMARKABLE: 0 0 0 0 1 1 0 0 0 5 1 1 --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, SUBACUTE --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE --N-CARCINOMA 103414001001 214412147254 111111002122 000000001000 001000000000 100000001100 000000000001 000000100000 490 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 32 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 1 0 1 3 2 4 1 1 0 0 4 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION / NECROSIS, FAT --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --HEMATOMA --INFLAMMATION, VASCULAR --THROMBUS --M-LIPOSARCOMA 101202010031 000010000000 100000000010 000010000000 000102000000 000101000000 000000100000 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 1 2 0 0 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-CARCINOMA --I-SARCOMA 011000001000 001000000000 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 0 1 2 0 1 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 012012000000 002001000000 FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 2 2 3 6 4 2 2 3 2 2 5 2 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 123642232252 123421122221 491 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 33 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 2 2 3 6 4 2 2 3 2 2 5 2 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 0 0 --ACANTHOSIS / HYPERKERATOSIS --B-FIBROMA --FIBROSIS --BACTERIA --HYPERPLASIA, BONE 013431132242 000000100000 000011011011 010110010010 000001000010 AORTA (AO) ........................................... NUMBER EXAMINED: 0 1 3 2 2 1 0 0 0 1 0 0 NOT REMARKABLE: 0 0 1 0 0 1 0 0 0 0 0 0 --M-LIPOSARCOMA --MINERALIZATION 000010000000 012210000100 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 1 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --I-SARCOMA 011000000000 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 0 1 2 0 1 2 1 1 1 0 1 0 NOT REMARKABLE: 0 0 0 0 1 1 0 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 011000111010 002000110010 002001011000 492 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 34 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- HEAD, CORONAL (HC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE, EAR 000000000100 JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 1 1 3 1 1 3 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --PROBABLE FRACTURE --FIBROSIS --HYPERPLASIA, BONE 113113000010 001000000000 100001000000 000001000000 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 0 0 0 0 0 1 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --ECTASIA, DUCTAL --INFLAMMATION, CHRONIC-ACTIVE --HYPERKERATOSIS 000000110000 000000010000 000000010000 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 1 0 0 --UNILATERALLY EXAMINED 000001000000 493 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 35 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 1 0 0 0 1 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 1 0 0 0 0 --M-LIPOSARCOMA --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS 000000100000 001000000000 001000000000 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 0 0 0 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --I-LIPOSARCOMA 000000100000 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 1 0 0 0 0 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --B-HIBERNOMA --B-AORTIC BODY TUMOR 000000100000 100000000000 LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 0 0 0 1 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --DILATATION, SINUSOIDAL --HEMORRHAGE --N-SARCOMA 000000001000 000001000000 000001000000 000001000000 494 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 36 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 0 1 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, PLASMACYTIC 000000001000 000001000001 MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC 001000000000 001000000000 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 0 0 1 0 0 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA, UNDIFFERENTIATED --N-SARCOMA 000001000000 001000000000 PINNA (PN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA --B-PAPILLOMA, SQUAMOUS CELL 000001000000 000000000001 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001000000000 495 TABLE 40 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 All Animals PAGE: 37 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: ------------MALE------------ -----------FEMALE----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001000000000 LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 000000000100 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 1 0 0 0 0 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --CYST --INFLAMMATION, CHRONIC --THROMBUS 001000000000 001000000000 001000000000 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 0 0 0 1 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --GANGLION ** END OF LIST ** 000100000000 496 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- ** TOP OF LIST ** EYE (EY) ............................................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION 01 BRAIN (BR) ........................................... NUMBER EXAMINED: 4 7 NOT REMARKABLE: 1 2 --DILATATION, VENTRICULAR --COMPRESSION, VENTRAL --I-CARCINOMA 11 34 01 LIVER (LI) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL --INFILTRATE, MACROPHAGE, PIGMENTED CELL 9 16 12 26 58 57 31 11 01 89 33 24 01 497 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 0 0 --VACUOLATION, HEPATOCELLULAR --CYST, BILIARY --ANGIECTASIS --B-ADENOMA, HEPATOCELLULAR 21 02 02 01 LIVER-OIL RED O (LI0) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 KIDNEY (KD) .......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 0 1 --NEPHROPATHY, CHRONIC PROGRESSIVE --DILATATION, PELVIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --CYST 98 11 1 13 01 03 01 10 SPLEEN (SP) .......................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --FIBROSIS, CAPSULAR 10 10 498 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- PANCREAS (PA) ........................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 1 11 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR 12 01 10 10 20 10 10 34 10 ADRENAL, CORTEX (AC) ................................. NUMBER EXAMINED: 0 3 NOT REMARKABLE: 0 0 --HYPERTROPHY, CORTICAL CELL --DEGENERATION, CYSTIC / HEMORRHAGIC --PIGMENT --DEGENERATION / NECROSIS, UNILATERAL --M-CARCINOMA 01 02 03 01 01 ADRENAL, MEDULLA (MA) ................................ NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 1 499 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- LN, MESENTERIC (MS) .................................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED 01 OVARY (OV) ........................................... NUMBER EXAMINED: 0 8 NOT REMARKABLE: 0 2 --CYST --HYPERPLASIA, GRANULOSA CELL 04 02 TESTIS (TE) .......................................... NUMBER EXAMINED: 9 0 NOT REMARKABLE: 8 0 --HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL) 10 BONE, FEMUR (FE) ..................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 MARROW, FEMUR (FM) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 MARROW, STERNUM (SE) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 500 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- BONE, STERNUM (SB) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SPINAL CORD (SC) ..................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 THYMUS (TH) .......................................... NUMBER EXAMINED: 0 2 NOT REMARKABLE: 0 0 --CYST --HYPERPLASIA, LYMPHORETICULAR --HYPERPLASIA, EPITHELIAL 01 01 01 LUNG (LU) ............................................ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 3 9 --MINERALIZATION, VASCULAR --N-CARCINOMA --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --MINERALIZATION, ALVEOLAR LUMINA --INFILTRATE, MACROPHAGE, PIGMENTED 32 01 53 11 01 01 HEART (HT) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 501 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- MUSCLE, SKELETAL (SM) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 NERVE, SCIATIC (SN) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 TRACHEA (TR) ......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 ESOPHAGUS (ES) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 THYROID (TY) ......................................... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 2 7 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL 25 10 10 24 51 01 502 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- PARATHYROID (PT) ..................................... NUMBER EXAMINED: 10 16 NOT REMARKABLE: 8 16 --HYPERPLASIA 20 PITUITARY (PI) ....................................... NUMBER EXAMINED: 8 11 NOT REMARKABLE: 0 0 --B-ADENOMA --HYPERPLASIA --CYST --M-CARCINOMA 79 13 01 01 HARDERIAN GLAND (HG) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 SALIV GL, MANDIB (SG) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 STOMACH, GL (ST) ..................................... NUMBER EXAMINED: 4 4 NOT REMARKABLE: 1 2 --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR 01 11 10 30 503 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- STOMACH, NONGL (SU) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 DUODENUM (DU) ........................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 JEJUNUM (JE) ......................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --M-CARCINOMA 01 ILEUM (IL) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CECUM (CE) ........................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 1 0 COLON (CO) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 RECTUM (RE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 504 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- SKIN (SK) ............................................ NUMBER EXAMINED: 3 2 NOT REMARKABLE: 0 0 --INFLAMMATION, CHRONIC-ACTIVE --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL 11 21 11 MAMMARY, FEMALE (MF) ................................. NUMBER EXAMINED: 0 17 NOT REMARKABLE: 0 0 --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --B-ADENOMA 01 07 02 07 0 11 02 URINARY BLADDER (UB) ................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 15 --HEMORRHAGE --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERPLASIA, TRANSITIONAL CELL 01 12 01 UTERUS (UT) .......................................... NUMBER EXAMINED: 0 6 NOT REMARKABLE: 0 1 --DILATATION 02 505 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) .......................................... NUMBER EXAMINED: 0 6 NOT REMARKABLE: 0 1 --B-POLYP, ENDOMETRIAL, STROMAL --HYPERPLASIA, CYSTIC, ENDOMETRIAL --M-LEIOMYOSARCOMA 01 03 01 CERVIX (CV) .......................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --B-POLYP, STROMAL 01 VAGINA (VA) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PROSTATE (PR) ........................................ NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --INFLAMMATION, CHRONIC-ACTIVE 10 SEMINAL VESICLES (SV) ................................ NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --SECRETION, DECREASED 10 506 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- EPIDIDYMIDES (EP) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 HEMATO NEOPLASIA (HN) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAUSE OF DEATH (CD) .................................. NUMBER EXAMINED: 10 17 NOT REMARKABLE: 0 0 --SCHEDULED SACRIFICE 10 17 LN, MEDIASTINAL (ML) ................................. NUMBER EXAMINED: 2 1 NOT REMARKABLE: 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --LYMPHANGIECTASIS 11 10 LN, AXILLARY (AX) .................................... NUMBER EXAMINED: 1 4 NOT REMARKABLE: 1 2 --HYPERPLASIA, LYMPHOPLASMACYTIC 02 LN, INGUINAL (IN) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 507 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- LN, OTHER (LN) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, RENAL (RL) ....................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED --LYMPHANGIECTASIS 10 10 LN, MANDIBULAR (MN) .................................. NUMBER EXAMINED: 1 5 NOT REMARKABLE: 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --LYMPHANGIECTASIS 13 14 10 VERTEBRA/MARROW (VT) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 MAMMARY, MALE (MM) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 COAGULATING GL (CG) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 508 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- TAIL (TI) ............................................ NUMBER EXAMINED: 2 0 NOT REMARKABLE: 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ACANTHOSIS / HYPERKERATOSIS --SUPERFICIAL CRUSTING 20 20 10 LN, LUMBAR/ILIAC (LM) ................................ NUMBER EXAMINED: 2 4 NOT REMARKABLE: 0 1 --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE 21 02 01 CAVITY, ABDOM (PC) ................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --INFLAMMATION, VASCULAR 10 AUDITORY SEB GL (AS) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PREPUTIAL GLAND (PG) ................................. NUMBER EXAMINED: 2 0 NOT REMARKABLE: 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 20 10 509 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- FOOT/FOOTPAD (FP) .................................... NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --ACANTHOSIS / HYPERKERATOSIS --FIBROSIS --HYPERPLASIA, BONE 11 01 01 11 10 AORTA (AO) ........................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CALVARIUM/SKULL (CA) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, ANT MES/PANC (AP) ................................ NUMBER EXAMINED: 1 0 NOT REMARKABLE: 1 0 HEAD, CORONAL (HC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 510 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- JOINT, OTHER (JT) .................................... NUMBER EXAMINED: 3 0 NOT REMARKABLE: 0 0 --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS --HYPERPLASIA, BONE 30 10 10 CLITORAL GLAND (CL) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 URETER (UE) .......................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 ADIPOSE TISSUE (AT) .................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 DIAPHRAGM (DP) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 CAVITY, THORACIC (TA) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 511 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- LN, TRACHEOBRON (TB) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, POPLITEAL (PP) ................................... NUMBER EXAMINED: 1 0 NOT REMARKABLE: 0 0 --HYPERPLASIA, PLASMACYTIC 10 MUSCLE, OTHER (MO) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PERICARDIAL SAC (PD) ................................. NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 PINNA (PN) ........................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --B-PAPILLOMA, SQUAMOUS CELL 01 ARTERY, OTHER (AU) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 512 TABLE 41 Incidence of Microscopic Observations Covance 6329-183 3M T-6295 Recovery Sacrifice PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL --- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ------------------------------------------------------------------------ -=- -=- AORTIC ARCH (AA) ..................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 LN, ILEOCECAL (IC) ................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 VAS DEFERENS (VS) .................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 BILE DUCT (BD) ....................................... NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 TRIGEMINAL NERVE (TG) ................................ NUMBER EXAMINED: 0 0 NOT REMARKABLE: 0 0 ** END OF LIST ** 513 APPENDIX 1 Protocol Deviations Covance 6329-183 3M T-6295 514 Protocol Deviations Covance 6329-183 3M T-6295 Protocol. Husbandry. Diet. "Certified Rodent Diet #5002, meal (PMI Nutrition International) ad libitum." Actual Procedure. During the course of the study, for a total of 29 incidences, 24 animals were found without access to food. Food was provided when the limited access was noted. Comments regarding limited access to food are recorded in the data. During single housing on April 13, 1998 (acclimation), temporary Animal No. 593 was not fed in. Protocol. Husbandry. Water. "Ad libitum." Actual Procedure. On April 29, 1998, during the a.m. room check it was noticed that Animal Nos. C92671 through C92690 (Group 3 males), and C92691 through C92725 (Group 4 males) did not have access to water due to a kinked water hose. Protocol. Husbandry. Housing. "Individual (may be group-housed during acclimation)." Actual Procedure. On May 10, 1998, Animal No. C92989 (Group 2 female) was found out of its cage during the a.m. check. Protocol. Termination. Unscheduled Sacrifices and Deaths. "Animals to be sacrificed will be anesthetized with carbon dioxide, weighed, and exsanguinated." Actual Procedure. At necropsy, Animal Nos. C92918 (Group 1 female), C92939 (Group 2 female), and C93038 (Group 3 female) were anesthetized with sodium pentobarbital instead of carbon dioxide. At necropsy, Animal No. C92621 received approximately 1.0mL of sodium pentobarbital prior to administration of carbon dioxide anesthesia. A body weight was not taken for Animal Nos. C92566 (Group 1 male), C92687 (Group 3 male) and C92966 (Group 2 female) prior to necropsy. 515 Protocol Deviations (Continued) Covance 6329-183 3M T-6295 Protocol. Postmortem Procedures. Tissue Preservation. "At the terminal sacrifice, sections of the heart and liver will be collected from 10 animals/sex/group in Groups 1, 4, 5, and 6 and preserved in 2.0% paraformaldehyde/2.5% glutaraldehyde in 0.1 M phosphate buffer. These tissues will be processed and embedded in epoxy blocks for possible future examination by electron microscopy. Actual Procedure. At the terminal sacrifice, tissues were collected from 11 animals in Group 4 (females) for possible electron microscopy instead of 10 animals. Protocol. Postmortem Procedures. Histopathology. "Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, and mammary glands (females) from each animal in Groups 2, 3, 4, and 6 sacrificed at the terminal and recovery sacrifices will be embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically." Actual Procedure. At the terminal sacrifice, one mammary gland mass was not collected for Animal Nos. C92927 (Group 1 female) and C93078 (Group 4 female). At necropsy, the mass located in the inguinal region for Animal No. C92729 was not commented on or collected. On July 13, 1999, a macroscopic lesion for Animal No. C92838 was not collected at the time of necropsy. At the terminal and recovery sacrifices, parathyroid glands were examined microscopically from animals in Groups 2, 3, 4. Protocol. Postmortem Procedures. Histopathology. "Slides of the pancreas from males found dead, sacrificed at an unscheduled interval, or sacrificed at the Week 14 and 53 interim sacrifices, terminal, and recovery sacrifices will be sent to Tox Path Inc. for peer-review and results will be provided to Covance for inclusion in the final report." Actual Procedure. Results of the peer-review are not included in the final report. The results are on file with Covance. 516 Protocol Deviations (Continued) Covance 6329-183 3M T-6295 Protocol. Experimental Design. Postmortem Procedures. "The following tissues (when present) or representative samples will be collected from each animal and preserved in 10% neutral-buffered formalin, unless otherwise specified." Actual Procedure. Some tissues, required by the protocol, were not available for histopathologic examination. Missing tissues are listed with appropriate comments in the pathology data sheets for individual animals. Summary tables do not include them as having been examined. These deviations are not expected to have affected the results of the study. 517 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume II of IX Page 518 of 4068 Covance 6329-183 3M T-6295 VOLUME II OF IX CONTENTS Page APPENDIX 2.............................................................................................................. 520 Individual Animal Fate Data.................................................................................... 521 Individual Clinical Observations.............................................................................. 543 519 APPENDIX 2 Individual Animal Fate Data Individual Clinical Observations Covance 6329-183 3M T-6295 520 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92501 C92502 C92503 C92504 C92505 C92506 C92507 C92508 C92509 C92510 C92511 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92521 C92522 C92523 C92524 C92525 C92526 C92527 C92528 C92529 C92530 C92531 C92532 C92533 C92534 DOSE GROUP ----- 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- 3 M T 3 T 1 3 D 2 T 2 3 T T D D 3 T D D 2 M 3 1 M 1 M 2 1 M M 2 M 3 TYPE OF DEATH ----------SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE DATE OF DEATH -------04/20/99 10/04/99 04/21/00 04/20/99 04/19/00 05/15/98 04/20/99 10/08/99 07/21/98 04/20/00 07/21/98 04/20/99 04/20/00 04/21/00 03/12/00 03/11/99 04/20/99 04/19/00 10/28/99 11/18/99 07/21/98 02/29/00 04/20/99 05/15/98 08/30/99 05/15/98 11/24/99 07/21/98 05/15/98 10/18/99 08/03/99 07/21/98 11/29/99 04/20/99 DAY OF STUDY ------366 533 733 366 731 26 366 537 93 732 93 366 732 733 693 326 366 731 557 578 93 681 366 26 498 26 584 93 26 547 471 93 589 366 WEEK OF STUDY ------53 77 105 53 105 4 53 77 14 105 14 53 105 105 99 47 53 105 80 83 14 98 53 4 72 4 84 14 4 79 68 14 85 53 521 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92535 C92536 C92537 C92538 C92539 C92540 C92541 C92542 C92543 C92544 C92545 C92546 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 C92563 C92564 C92565 C92566 C92567 C92568 DOSE GROUP ----- 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M M D D M 3 T T D T D 1 M D D D T M M M M M M M D M M 3 M M D M D D TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------02/14/00 10/17/99 02/14/00 09/14/99 02/28/00 04/20/99 04/20/00 04/21/00 03/14/00 04/20/00 01/31/00 05/15/98 12/21/99 11/22/99 10/31/99 04/15/00 04/19/00 04/14/00 06/28/99 04/07/00 06/08/99 06/28/99 12/27/99 10/28/99 10/22/99 10/31/99 06/29/99 04/20/99 11/24/99 08/19/99 01/16/00 09/21/98 01/12/00 03/20/00 DAY OF STUDY ------666 546 666 513 680 366 732 733 695 732 652 26 611 582 560 727 731 726 435 719 415 435 617 557 551 560 436 366 584 487 637 155 633 701 WEEK OF STUDY ------96 78 96 74 98 53 105 105 100 105 94 4 88 84 80 104 105 104 63 103 60 63 89 80 79 80 63 53 84 70 91 23 91 101 522 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92569 C92570 C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 C92602 DOSE GROUP ----- 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- T 3 M D M 1 1 M T M D M M M T M M M M M M T D M 2 D M M T T D 2 T T TYPE OF DEATH ----------SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE DATE OF DEATH -------04/19/00 04/20/99 03/21/00 03/21/00 11/24/99 05/15/98 05/15/98 02/21/00 04/21/00 12/08/99 12/12/99 10/20/99 04/17/00 03/21/00 04/19/00 06/11/99 01/12/99 09/07/99 12/20/99 04/03/00 03/13/00 04/20/00 02/18/00 12/20/99 07/21/98 02/15/00 11/23/99 08/23/99 04/19/00 04/19/00 05/09/99 07/21/98 04/21/00 04/21/00 DAY OF STUDY ------731 366 702 702 584 26 26 673 733 598 602 549 729 702 731 418 268 506 610 715 694 732 670 610 93 667 583 491 731 731 385 93 733 733 WEEK OF STUDY ------105 53 101 101 84 4 4 97 105 86 86 79 105 101 105 60 39 73 88 103 100 105 96 88 14 96 84 71 105 105 55 14 105 105 523 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 C92631 C92632 C92633 C92634 C92635 C92636 DOSE GROUP ----- 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- M M D M M M M 1 M M 1 D M 2 M 1 T D 2 D T D M T 2 M D M T M T D M D TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------08/24/98 01/10/00 04/11/00 10/13/99 01/10/00 10/19/99 04/12/00 05/15/98 12/20/99 10/18/99 05/15/98 01/19/00 01/03/00 07/21/98 11/05/99 05/15/98 04/20/00 02/15/00 07/21/98 11/18/99 04/21/00 06/18/99 11/28/99 04/20/00 07/21/98 09/07/99 10/17/99 12/26/99 04/19/00 04/03/00 04/20/00 12/13/99 10/20/99 03/01/00 DAY OF STUDY ------127 631 723 542 631 548 724 26 610 547 26 640 624 93 565 26 732 667 93 578 733 425 588 732 93 506 546 616 731 715 732 603 549 682 WEEK OF STUDY ------19 91 104 78 91 79 104 4 88 79 4 92 90 14 81 4 105 96 14 83 105 61 84 105 14 73 78 88 105 103 105 87 79 98 524 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 DOSE GROUP ----- 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- T M M 2 T T T T 2 1 M M T 1 M 1 T M M M T D T T M 2 D D D M M 1 T M TYPE OF DEATH ----------SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------04/19/00 01/10/00 11/29/99 07/21/98 04/20/00 04/19/00 04/21/00 04/19/00 07/21/98 05/15/98 10/28/99 12/15/99 04/20/00 05/15/98 11/01/99 05/15/98 04/21/00 10/20/99 02/01/00 02/21/00 04/21/00 02/01/00 04/20/00 04/20/00 01/03/00 07/21/98 02/08/00 10/11/99 12/24/99 10/01/99 10/20/99 05/15/98 04/20/00 09/13/99 DAY OF STUDY ------731 631 589 93 732 731 733 731 93 26 557 605 732 26 561 26 733 549 653 673 733 653 732 732 624 93 660 540 614 530 549 26 732 512 WEEK OF STUDY ------105 91 85 14 105 105 105 105 14 4 80 87 105 4 81 4 105 79 94 97 105 94 105 105 90 14 95 78 88 76 79 4 105 74 525 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 DOSE GROUP ----- 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- D M D T M 2 D 1 M M M T T 2 M M M M D T T D T T T T M T T T M T D T TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE DATE OF DEATH -------01/29/00 02/13/00 02/07/00 04/19/00 03/31/00 07/21/98 03/09/00 05/15/98 05/07/99 11/23/99 12/16/99 04/19/00 04/21/00 07/21/98 10/25/99 11/22/99 09/03/99 02/25/00 05/14/99 04/21/00 04/19/00 04/19/99 04/21/00 04/19/00 04/19/00 04/19/00 07/28/99 04/20/00 04/19/00 04/21/00 04/19/99 04/20/00 06/16/99 04/20/00 DAY OF STUDY ------650 665 659 731 712 93 690 26 383 583 606 731 733 93 554 582 502 677 390 733 731 365 733 731 731 731 465 732 731 733 365 732 423 732 WEEK OF STUDY ------93 95 95 105 102 14 99 4 55 84 87 105 105 14 80 84 72 97 56 105 105 53 105 105 105 105 67 105 105 105 53 105 61 105 526 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 DOSE GROUP ----- 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- T M M T D D D M 2 2 1 T T 1 2 D M 2 M T D T D 2 M T 1 D M 1 T T M D TYPE OF DEATH ----------SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------04/19/00 02/28/00 01/06/00 04/19/00 01/12/00 08/18/99 01/19/00 12/13/99 07/21/98 07/21/98 05/15/98 04/20/00 04/21/00 05/15/98 07/21/98 11/16/99 10/28/99 07/21/98 10/23/99 04/19/00 04/17/99 04/21/00 01/09/00 07/21/98 10/20/99 04/20/00 05/15/98 11/18/99 12/27/99 05/15/98 04/19/00 04/21/00 01/16/00 01/20/00 DAY OF STUDY ------731 680 627 731 633 486 640 603 93 93 26 732 733 26 93 576 557 93 552 731 363 733 630 93 549 732 26 578 617 26 731 733 637 641 WEEK OF STUDY ------105 98 90 105 91 70 92 87 14 14 4 105 105 4 14 83 80 14 79 105 52 105 90 14 79 105 4 83 89 4 105 105 91 92 527 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 DOSE GROUP ----- 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- T T M T D 1 M M T T D T 3 1 T 3 3 M T M 1 M T T M M 2 D D M D D M M TYPE OF DEATH ----------SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------04/21/00 04/21/00 01/12/00 04/20/00 11/27/99 05/15/98 01/03/00 10/22/99 04/20/00 04/20/00 04/06/00 04/20/00 04/20/99 05/15/98 04/21/00 04/20/99 04/20/99 12/24/99 04/19/00 06/21/99 05/15/98 09/13/99 04/20/00 04/19/00 02/01/00 10/01/99 07/21/98 03/24/00 03/10/00 02/08/00 04/09/00 03/21/00 11/07/99 04/14/99 DAY OF STUDY ------733 733 633 732 587 26 624 551 732 732 718 732 366 26 733 366 366 614 731 428 26 512 732 731 653 530 93 705 691 660 721 702 567 360 WEEK OF STUDY ------105 105 91 105 84 4 90 79 105 105 103 105 53 4 105 53 53 88 105 62 4 74 105 105 94 76 14 101 99 95 103 101 81 52 528 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- D T T T 2 3 1 T M T M M D T D T 2 3 T T 1 D 3 3 M 1 2 T D T T T M M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE THIRD INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE SECOND INTERIM SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------04/19/00 04/21/00 04/20/00 04/20/00 07/21/98 04/20/99 05/15/98 04/21/00 10/06/99 04/20/00 10/18/99 10/27/99 03/05/00 04/19/00 03/13/99 04/21/00 07/21/98 04/20/99 04/19/00 04/20/00 05/15/98 05/26/99 04/20/99 04/20/99 02/01/00 05/15/98 07/21/98 04/19/00 05/17/99 04/19/00 04/21/00 04/19/00 09/01/99 03/10/00 DAY OF STUDY ------731 733 732 732 93 366 26 733 535 732 547 556 686 731 328 733 93 366 731 732 26 402 366 366 653 26 93 731 393 731 733 731 500 691 WEEK OF STUDY ------105 105 105 105 14 53 4 105 77 105 79 80 98 105 47 105 14 53 105 105 4 58 53 53 94 4 14 105 57 105 105 105 72 99 529 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92807 C92808 C92809 C92810 C92811 C92812 C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE ----- D T T M M 2 M T T D 3 T D 3 U M U D D D U D U M M D U U M D M M D M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------12/07/99 04/19/00 04/21/00 02/21/00 12/25/98 07/21/98 03/13/00 04/21/00 04/20/00 07/09/99 04/20/99 04/20/00 04/15/00 04/20/99 04/24/00 11/06/99 04/24/00 02/11/00 02/01/00 05/09/99 04/24/00 11/18/99 04/24/00 03/09/00 11/29/99 08/12/99 04/24/00 04/24/00 09/23/99 01/10/00 07/12/99 07/13/99 01/02/00 04/03/00 DAY OF STUDY ------597 731 733 673 250 93 694 733 732 446 366 732 727 366 736 566 736 663 653 385 736 578 736 690 589 480 736 736 522 631 449 450 623 715 WEEK OF STUDY ------86 105 105 97 36 14 100 105 105 64 53 105 104 53 106 81 106 95 94 55 106 83 106 99 85 69 106 106 75 91 65 65 89 103 530 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 C92861 C92862 C92863 C92864 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 DOSE GROUP ----- 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX ------ MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- D M D M M D M U M M U M U D M U M M M M 1 3 M 1 D T T T T M T M T T TYPE OF DEATH ----------UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE DATE OF DEATH -------08/08/99 01/12/00 10/08/99 11/19/99 02/12/00 04/09/00 02/28/00 04/24/00 08/16/99 04/18/00 04/24/00 08/07/99 04/24/00 03/24/00 08/16/99 04/24/00 11/16/99 02/21/00 09/01/99 02/26/00 05/15/98 04/20/99 11/15/99 05/15/98 07/12/99 04/21/00 04/20/00 04/19/00 04/20/00 04/17/00 04/19/00 12/28/99 04/21/00 04/19/00 DAY OF STUDY ------476 633 537 579 664 721 680 736 484 730 736 475 736 705 484 736 576 673 500 678 26 366 575 26 449 733 732 731 732 729 731 618 733 731 WEEK OF STUDY ------68 91 77 83 95 103 98 106 70 105 106 68 106 101 70 106 83 97 72 97 4 53 83 4 65 105 105 105 105 105 105 89 105 105 531 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92875 C92876 C92877 C92878 C92879 C92880 C92881 C92882 C92883 C92884 C92885 C92886 C92887 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92898 C92899 C92900 C92901 C92902 C92903 C92904 C92905 C92906 C92907 C92908 DOSE GROUP ----- 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M 3 T M M 2 T T M M T M 2 M 3 T M T M 3 T T T 2 3 D M D 2 M 2 T T M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------10/24/99 04/20/99 04/20/00 11/02/99 01/24/00 07/21/98 04/21/00 04/21/00 12/14/99 02/14/00 04/21/00 11/01/99 07/21/98 10/24/99 04/20/99 04/21/00 12/01/99 04/20/00 12/20/99 04/20/99 04/20/00 04/19/00 04/19/00 07/21/98 04/20/99 09/05/99 10/22/99 12/27/99 07/21/98 03/21/00 07/21/98 04/19/00 04/21/00 01/25/00 DAY OF STUDY ------553 366 732 562 645 93 733 733 604 666 733 561 93 553 366 733 591 732 610 366 732 731 731 93 366 504 551 617 93 702 93 731 733 646 WEEK OF STUDY ------79 53 105 81 93 14 105 105 87 96 105 81 14 79 53 105 85 105 88 53 105 105 105 14 53 72 79 89 14 101 14 105 105 93 532 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92909 C92910 C92911 C92912 C92913 C92914 C92915 C92916 C92917 C92918 C92919 C92920 C92921 C92922 C92923 C92924 C92925 C92926 C92927 C92928 C92929 C92930 C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92941 C92942 DOSE GROUP ----- 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- T 3 T T T 1 M M 3 M 1 M M 3 3 M T 1 T M M 3 D M M M M T M T M M 1 M TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------04/20/00 04/20/99 04/20/00 04/20/00 04/19/00 05/15/98 02/18/00 01/28/00 04/20/99 09/09/99 05/15/98 08/30/99 05/17/99 04/20/99 04/20/99 11/29/99 04/21/00 05/15/98 04/19/00 11/15/99 06/08/99 04/20/99 08/22/99 01/29/99 01/21/00 12/13/99 12/22/99 04/20/00 07/13/99 04/20/00 08/23/99 12/13/99 05/15/98 04/02/99 DAY OF STUDY ------732 366 732 732 731 26 670 649 366 508 26 498 393 366 366 589 733 26 731 575 415 366 490 285 642 603 612 732 450 732 491 603 26 348 WEEK OF STUDY ------105 53 105 105 105 4 96 93 53 73 4 72 57 53 53 85 105 4 105 83 60 53 70 41 92 87 88 105 65 105 71 87 4 50 533 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 C92970 C92971 C92972 C92973 C92974 C92975 C92976 DOSE GROUP ----- 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M 2 1 T M M M 1 M M T M M M T M M T M 2 M T D M 2 D 1 T M M T T T M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------02/25/00 07/21/98 05/15/98 04/20/00 02/04/00 03/10/00 12/04/98 05/15/98 04/03/00 07/19/99 04/21/00 03/23/00 01/17/00 03/28/00 04/21/00 12/29/99 02/28/00 04/21/00 03/10/00 07/21/98 02/04/00 04/19/00 11/13/99 09/03/99 07/21/98 08/09/99 05/15/98 04/19/00 01/03/00 02/18/00 04/19/00 04/20/00 04/21/00 03/31/00 DAY OF STUDY ------677 93 26 732 656 691 229 26 715 456 733 704 638 709 733 619 680 733 691 93 656 731 573 502 93 477 26 731 624 670 731 732 733 712 WEEK OF STUDY ------97 14 4 105 94 99 33 4 103 66 105 101 92 102 105 89 98 105 99 14 94 105 82 72 14 69 4 105 90 96 105 105 105 102 534 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 C92991 C92992 C92993 C92994 C92995 C92996 C92997 C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 DOSE GROUP ----- 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- D 2 M M M 1 T T M T 2 T M M T M 2 M M 1 M M D 2 M M T M M 1 T 1 M M TYPE OF DEATH ----------UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE SECOND INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------03/05/00 07/21/98 01/01/00 06/28/99 09/03/99 05/15/98 04/19/00 04/20/00 02/28/00 04/21/00 07/21/98 04/19/00 11/22/99 02/01/00 04/07/00 11/23/99 07/21/98 11/12/99 06/17/98 05/15/98 11/15/99 03/01/99 01/22/00 07/21/98 11/24/99 11/01/99 04/07/00 09/21/99 01/09/00 05/15/98 04/07/00 05/15/98 01/21/00 01/10/00 DAY OF STUDY ------686 93 622 435 502 26 731 732 680 733 93 731 582 653 719 583 93 572 59 26 575 316 643 93 584 561 719 520 630 26 719 26 642 631 WEEK OF STUDY ------98 14 89 63 72 4 105 105 98 105 14 105 84 94 103 84 14 82 9 4 83 46 92 14 84 81 103 75 90 4 103 4 92 91 535 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 DOSE GROUP ----- 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- T M M M M D M 2 M D M T 2 M D M M M M M 1 M M D 2 D M M T T M M M M TYPE OF DEATH ----------SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------04/07/00 11/01/99 12/06/99 10/01/99 03/31/00 11/11/99 01/10/00 07/21/98 02/22/99 07/24/99 06/28/99 04/07/00 07/21/98 12/08/99 03/23/00 04/03/00 01/10/00 01/17/00 09/01/99 02/18/00 05/15/98 10/27/99 07/19/99 11/15/99 07/21/98 12/02/99 07/22/99 08/23/99 04/07/00 04/07/00 11/15/99 02/13/00 02/21/00 07/19/99 DAY OF STUDY ------719 561 596 530 712 571 631 93 309 461 435 719 93 598 704 715 631 638 500 670 26 556 456 575 93 592 459 491 719 719 575 665 673 456 WEEK OF STUDY ------103 81 86 76 102 82 91 14 45 66 63 103 14 86 101 103 91 92 72 96 4 80 66 83 14 85 66 71 103 103 83 95 97 66 536 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C93045 C93046 C93047 C93048 C93049 C93050 C93051 C93052 C93053 C93054 C93055 C93056 C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 DOSE GROUP ----- 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 1 M T T M M 2 M M M M M M 1 T T D T T 2 M M 1 D M 1 2 T D M 1 M T T TYPE OF DEATH ----------SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE DATE OF DEATH -------05/15/98 10/25/99 04/07/00 04/07/00 08/16/99 03/02/00 07/21/98 09/01/99 07/22/99 09/05/99 10/04/99 03/10/00 03/10/00 05/15/98 04/20/00 04/21/00 10/29/99 04/19/00 04/19/00 07/21/98 01/24/00 01/24/00 05/15/98 04/06/00 03/21/00 05/15/98 07/21/98 04/20/00 09/23/99 11/15/99 05/15/98 02/01/00 04/21/00 04/19/00 DAY OF STUDY ------26 554 719 719 484 683 93 500 459 504 533 691 691 26 732 733 558 731 731 93 645 645 26 718 702 26 93 732 522 575 26 653 733 731 WEEK OF STUDY ------4 80 103 103 70 98 14 72 66 72 77 99 99 4 105 105 80 105 105 14 93 93 4 103 101 4 14 105 75 83 4 94 105 105 537 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 C93111 C93112 DOSE GROUP ----- 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 5 5 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- T T T D M 1 2 M M D M T M M M M D D M T M D T T M M D M T D 2 M 2 3 TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE THIRD INTERIM SACRIFICE DATE OF DEATH -------04/20/00 04/21/00 04/20/00 02/25/00 04/17/00 05/15/98 07/21/98 01/09/00 03/16/00 10/15/99 02/04/00 04/19/00 10/06/99 10/06/99 02/18/00 02/18/00 04/17/00 11/22/98 02/28/00 04/20/00 02/19/00 12/28/99 04/20/00 04/21/00 03/10/00 11/23/99 12/28/99 02/09/00 04/19/00 04/18/99 07/21/98 01/09/00 07/21/98 04/20/99 DAY OF STUDY ------732 733 732 677 729 26 93 630 697 544 656 731 535 535 670 670 729 217 680 732 671 618 732 733 691 583 618 661 731 364 93 630 93 366 WEEK OF STUDY ------105 105 105 97 105 4 14 90 100 78 94 105 77 77 96 96 105 31 98 105 96 89 105 105 99 84 89 95 105 52 14 90 14 53 538 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C93113 C93114 C93115 C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- T 1 T 3 3 M M 3 M M 1 T T M 2 M D 3 M M T D T D T 3 T T M 1 2 1 D T TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------TERMINAL SACRIFICE FIRST INTERIM SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE DATE OF DEATH -------04/19/00 05/15/98 04/20/00 04/20/99 04/20/99 01/24/00 01/17/00 04/20/99 10/20/99 01/21/00 05/15/98 04/20/00 04/19/00 05/30/99 07/21/98 03/13/00 03/13/00 04/20/99 02/14/00 04/17/00 04/21/00 08/05/99 04/19/00 05/09/99 04/20/00 04/20/99 04/21/00 04/20/00 03/17/00 05/15/98 07/21/98 05/15/98 09/24/99 04/20/00 DAY OF STUDY ------731 26 732 366 366 645 638 366 549 642 26 732 731 406 93 694 694 366 666 729 733 473 731 385 732 366 733 732 698 26 93 26 523 732 WEEK OF STUDY ------105 4 105 53 53 93 92 53 79 92 4 105 105 58 14 100 100 53 96 105 105 68 105 55 105 53 105 105 100 4 14 4 75 105 539 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 C93178 C93179 C93180 DOSE GROUP ----- 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- 3 D T M T M T 3 2 M T T 1 M T T 3 T T T M T 2 M 3 M M T M M T T T M TYPE OF DEATH ----------SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------THIRD INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE THIRD INTERIM SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE THIRD INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH THIRD INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH DATE OF DEATH -------04/20/99 01/15/00 04/19/00 01/19/00 04/21/00 07/19/99 04/20/00 04/20/99 07/21/98 12/06/99 04/21/00 04/19/00 05/15/98 09/27/99 04/19/00 04/19/00 04/20/99 04/19/00 04/21/00 04/20/00 01/12/99 04/20/00 07/21/98 02/01/00 04/20/99 10/17/99 03/28/00 04/19/00 01/20/00 12/23/99 04/21/00 04/21/00 04/21/00 06/28/99 DAY OF STUDY ------366 636 731 640 733 456 732 366 93 596 733 731 26 526 731 731 366 731 733 732 268 732 93 653 366 546 709 731 641 613 733 733 733 435 WEEK OF STUDY ------53 91 105 92 105 66 105 53 14 86 105 105 4 76 105 105 53 105 105 105 39 105 14 94 53 78 102 105 92 88 105 105 105 63 540 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 DOSE GROUP ----- 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- U U U U U M M D M U M U M M D M U U M M U U M D U M M D U M U D M D TYPE OF DEATH ----------SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED DESCRIPTION OF DEATH ------------------------FIRST POST-RECOV SAC FIRST POST-RECOV SAC FIRST POST-RECOV SAC FIRST POST-RECOV SAC FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH DATE OF DEATH -------04/24/00 04/24/00 04/24/00 04/24/00 04/24/00 09/21/99 04/13/00 04/22/00 12/15/99 04/24/00 01/12/00 04/24/00 10/01/99 01/17/00 08/23/99 12/24/99 04/24/00 04/24/00 07/13/99 02/19/00 04/24/00 04/24/00 01/03/00 01/21/00 04/24/00 04/15/00 01/25/99 11/16/99 04/24/00 04/22/00 04/24/00 11/24/99 11/26/99 03/03/00 DAY OF STUDY ------736 736 736 736 736 520 725 734 605 736 633 736 530 638 491 614 736 736 450 671 736 736 624 642 736 727 281 576 736 734 736 584 586 684 WEEK OF STUDY ------106 106 106 106 106 75 104 105 87 106 91 106 76 92 71 88 106 106 65 96 106 106 90 92 106 104 41 83 106 105 106 84 84 98 541 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER -----C93215 C93216 C93217 C93218 C93219 C93220 DOSE GROUP ----- 6 6 6 6 6 6 SEX -----FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE ----- M U D M U U TYPE OF DEATH ----------UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH ------------------------UNSCHEDULED DEATH FIRST POST-RECOV SAC UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST POST-RECOV SAC FIRST POST-RECOV SAC DATE OF DEATH -------12/06/99 04/24/00 01/14/00 01/03/00 04/24/00 04/24/00 DAY OF STUDY ------596 736 635 624 736 736 WEEK OF STUDY ------86 106 91 90 106 106 542 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92501 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C92502 M 77 APPEARANCE THIN 533 BEHAVIOR ATAXIC <UNSPECIFIED> 533 HYPOACTIVE <UNSPECIFIED> 533 DISCHARGE GENITAL RED IN COLOR 274, 281, 337 EYES RED DISCHARGE EYE-LEFT 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 309, 316, 407, 414, 498, 505, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 323, 330, 344, 351, 421, 428, 435, 442, 512, 519, 526 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 358, 365, 372, 379, 449, 456, 463, 470, 92, 99, 106 183, 190, 197 288, 295, 302 386, 393, 400 477, 484, 491 543 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92503 T 105 APPEARANCE THIN 715, 722, 729, 733 RESPIRATION AUDIBLE 575, 582, 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) TAIL-PROXIMAL WEEK 72 WEEK 105 498C, 575C, 652C, 729C, 729C, 505C, 582C, 659C, 733C 733C 512C, 589C, 666C, 519C, 596C, 673C, 526C, 603C, 680C, 533C, 610C, 687C, 540C, 617C, 694C, 547C, 624C, 701C, 554C, 631C, 708C, 561C, 638C, 715C, 568C 645C 722C C92504 3 53 APPEARANCE MALOCCLUSION MISSING TEETH 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365-366 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71 544 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92504 3 53 SURGERY PERFORMED BY KATHY EWERS 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92505 T 105 APPEARANCE MALOCCLUSION 673, 680, 687, 694, 701, 708, 715 EYES SQUINTED PERI-ORBITAL 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 722, 729, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 617, 731 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 624, 631, 638, 645, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 652, 659, 666 545 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92506 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92507 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C92508 D 77 SKIN & PELAGE ROUGH HAIR COAT 533, 537 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92509 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92510 T 105 APPEARANCE MALOCCLUSION 470, 477, 484, 512, 519, 526, 533, 561, 610, 617, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 491 546 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92510 T 105 NORMAL NO REMARKABLE OBSERVATIONS 498, 505, 540, 547, 554, 568, 575, 582, 589, 596, 603, 624, 631 638, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 C92511 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92512 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY KATHY EWERS 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92513 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 428, 435, 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 498, 505, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 512, 519, 526, 533, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 449, 456, 463, 470, 540, 547, 554, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 568, 575, 582 547 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92513 T 105 NORMAL NO REMARKABLE OBSERVATIONS 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 732 C92514 T 105 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-RIGHT 414, 421 617, 624, 631, 638, 645, 652 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 491, 498, 582, 589, 733 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 428, 435, 505, 512, 519, 526, 596, 603, 610, 659, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 442, 449, 456, 463, 533, 540, 547, 554, 666, 673, 680, 687, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 470, 477, 484 561, 568, 575 694, 701, 729 MASS(ES) TAIL-PROXIMAL WEEK 102 708, 715, 722 548 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92515 D 99 APPEARANCE MALOCCLUSION 512, 519, 526, 533, 540, 547, 554, 561 EYES SQUINTED PERI-ORBITAL 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 638, 645, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 568, 652, 659, 666, 673, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 575, 582, 589, 596, 680, 687, 693 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 617, 624, 631 C92516 D 47 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 326 C92517 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 549 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92518 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 651-652, 659, 666, 673, 680, 687 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 701, 708, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 715, 722, 729, 731 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 694 C92519 D 80 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 557 C92520 D 83 APPEARANCE LIMITED USE LIMB-HIND-RIGHT PAW-HIND-RIGHT SWOLLEN DIGIT(S)-HIND-RIGHT LIMB-HIND-RIGHT PAW-HIND-RIGHT 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 578 470, 477 533, 540, 547, 554, 561, 568, 575, 578 554, 561, 568 554, 561, 568 550 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92520 D 83 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 484 C92521 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92522 M 98 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 681 RECUMBENT LATERAL 681 EXCRETION FEW FECES 681 EYES RED DISCHARGE EYES 681 SQUINTED PERI-ORBITAL 681 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND VENTRAL-ABDOMINAL 624, 631 638, 645, 652, 659, 666, 673, 680-681 330, 337, 344, 351, 449, 456, 463, 519, 526, 533, 631, 680-681 551 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92522 M 98 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 470 477, 484, 491, 498, 505, 512, 540, 547, 554, 561, 568, 575, 582 589, 596 MASS(ES) VENTRAL-ABDOMINAL WEEK 63 WEEK 87 WEEK 97 VENTRAL-ABDOMINAL-LEFT WEEK 44 435C, 442C 603C, 610C, 617C, 624C 673 302C, 309C, 316C, 323C C92523 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C92524 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 552 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92525 M 72 APPEARANCE THIN 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 BEHAVIOR HYPOACTIVE MODERATE 498 EXCRETION FEW FECES NO FECES 428, 435, 470 498 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT 421, 428, 435, 442, 449, 456, 463 449, 456, 463, 470, 477, 484, 491, 498 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) VENTRAL-ABDOMINAL WEEK 56 WEEK 61 386 421, 428, 435, 442, 449, 456, 463, 470, 477C, 484C, 491C, 498C 553 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92526 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92527 M 84 APPEARANCE HEAD TILT RIGHT LIMITED USE LIMB-HIND-RIGHT THIN 575, 582, 584 584 582, 584 BEHAVIOR ATAXIC <UNSPECIFIED> 584 HYPOACTIVE <UNSPECIFIED> 584 EXCRETION FEW FECES 584 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT 561, 568, 575, 582 372, 379, 386, 393, 400, 407, 414 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 435, 442, 526, 533, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 449, 456, 463, 470, 540, 547, 554 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 477, 484, 491, 498, 92, 99, 106 183, 190, 197 274, 281, 288 365, 421, 428 505, 512, 519 554 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92528 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92529 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92530 M 79 BEHAVIOR RECUMBENT LATERAL 547 EXCRETION FEW FECES 547 RESPIRATION AUDIBLE 547 IRREGULAR 547 LABORED 547 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 555 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92531 M 68 BEHAVIOR RECUMBENT LATERAL 471 EXCRETION FEW FECES NO FECES 469-470 471 EYES RED DISCHARGE EYES 471 SQUINTED PERI-ORBITAL 471 RESPIRATION IRREGULAR 471 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463 C92532 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 556 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92533 M 85 SKIN & PELAGE RED SKIN HEAD-MAXILLARY-RIGHT SORE/SCAB HEAD-MAXILLARY-RIGHT 456 561, 568, 575, 582, 589 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 INGUINAL REGION-LEFT WEEK 69 491, 498, 505, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C 477, 484, 491, 498, 505, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C C92534 3 53 APPEARANCE MALOCCLUSION 365-366 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 557 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92534 3 53 SURGERY PERFORMED BY JOHN VAN BERKUM 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92535 M 96 APPEARANCE MALOCCLUSION 561 TREMORS LIMBS-ALL 666 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 666 COLD TO TOUCH ENTIRE BODY 666 DISCHARGE GENITAL RED IN COLOR 43 EXCRETION DISCOLORED FECES RED IN COLOR NONFORMED FECES 666 589, 666 EYES RED DISCHARGE EYES 302 558 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92535 M 96 EYES SQUINTED PERI-ORBITAL 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 309, 316, 400, 407, 491, 498, 596, 603, 22, 29, 36, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 323, 330, 337, 344, 414, 421, 428, 435, 505, 512, 519, 526, 610, 617, 624, 631, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 638, 645, 652, 659 99, 106, 113 190, 197, 204 281, 288, 295 379, 386, 393 470, 477, 484 568, 575, 582 SACRIFICE SACRIFICE APPROVED BY TOXICOLOGIST 666 C92536 M 78 APPEARANCE SWOLLEN NOSE 546 BEHAVIOR COLD TO TOUCH ENTIRE BODY 546 DISCHARGE GENITAL RED IN COLOR 337 RESPIRATION AUDIBLE 546 IRREGULAR 546 559 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92536 M 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540 C92537 D 96 EXCRETION FEW FECES 662, 666 SKIN & PELAGE SORE/SCAB DORSAL 645, 652, 659, 662, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) DORSAL WEEK 93 VENTRAL-CERVICAL-LEFT WEEK 91 645C, 652C, 659C, 662C, 666C 631C, 638C, 645C, 652C, 659C, 662C, 666C 560 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92538 D 74 APPEARANCE OBES 470, 477, 484, 491, 498, 505, 512-513 DISCHARGE GENITAL RED IN COLOR 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463 C92539 M 98 APPEARANCE THIN 673, 680 EXCRETION FEW FECES 673, 680 EYES RED DISCHARGE EYE-LEFT EYES 617, 624, 631, 638, 645, 652, 659 666, 673, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 561 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92539 M 98 NORMAL NO REMARKABLE OBSERVATIONS 568, 575, 582, 589, 596 MASS(ES) TAIL-PROXIMAL WEEK 87 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 C92540 3 53 APPEARANCE MALOCCLUSION 197, 204 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C92541 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 701, 708, 715, 722, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 729, 732 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 680 MASS(ES) DORSAL WEEK 68 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 562 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92541 T 105 WART LIKE LESION(S) NOSE WEEK 69 477, 484 C92542 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 100 DORSAL WEEK 103 TAIL-PROXIMAL WEEK 93 694, 701, 708 715C, 722C, 729C, 733C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 733C WART LIKE LESION(S) DORSAL-CERVICAL-RIGHT WEEK 69 HEAD-MAXILLARY-RIGHT WEEK 66 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 733 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 733 563 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92543 D 100 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 127, 134, 141, 148, 155, 162, 169, 176, 218, 225, 232, 239, 246, 253, 260, 267, 309, 316, 323, 330, 337, 344, 351, 358, 400, 407, 414, 421, 428, 435, 442, 449, 491, 498, 505, 512, 519, 526, 533, 540, 582, 589, 596, 603, 610, 617, 624, 631, 673, 680, 687, 694-695 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92544 T 105 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-RIGHT PAWS-HIND TAIL THIN 43, 50, 57, 59, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 732 708, 715, 722, 729, 732 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 732 729, 732 59, 435 EXCRETION FEW FECES 59, 435 SKIN & PELAGE ROUGH HAIR COAT 59 564 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92544 T 105 SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL 589, 596, 603, 610, 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 MASS(ES) LIMB-FRONT-LEFT WEEK 102 708C, 715C, 722C, 729C, 732C WART LIKE LESION(S) PAW-HIND-LEFT WEEK 95 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 C92545 D 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 540, 547, 631, 638, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 554, 561, 568, 575, 645, 652 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 582, 589, 596, 603, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 610, 617, 624 WART LIKE LESION(S) DORSAL WEEK 69 477, 484, 491, 498, 505, 512, 519, 526, 533 565 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92546 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92547 M 88 APPEARANCE HEAD TILT RIGHT THIN 611 610-611 BEHAVIOR ATAXIC <UNSPECIFIED> 611 HYPOACTIVE <UNSPECIFIED> 611 DISCHARGE GENITAL RED IN COLOR 449 EXCRETION NO FECES 611 EYES CLEAR DISCHARGE EYE-LEFT 15 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB DORSAL YELLOW HAIR COAT 610-611 554, 561, 568, 575, 582, 589, 596, 603, 610 611 566 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92547 M 88 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547 C92548 D 84 SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT 29, 36, 43, 50, 57 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 141, 148, 232, 239, 323, 330, 414, 421, 505, 512, 22, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106, 113, 120, 127, 134 183, 190, 197, 204, 211, 218, 225 274, 281, 288, 295, 302, 309, 316 365, 372, 379, 386, 393, 400, 407 456, 463, 470, 477, 484, 491, 498 547, 554, 561, 568, 575, 582 C92549 D 80 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 560 567 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92550 D 104 APPEARANCE SWOLLEN PAW-HIND-RIGHT 708, 715, 722, 727 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92551 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 379 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 400, 407, 491, 498, 582, 589, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 414, 421, 428, 435, 505, 512, 519, 526, 596, 603, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 442, 449, 456, 463, 533, 540, 547, 554, 624, 631, 638, 645, 715, 722, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 386, 393 470, 477, 484 561, 568, 575 652, 659, 666 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 372C 568 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92552 M 104 APPEARANCE HEAD TILT RIGHT THIN 722, 726 694, 701, 708, 715, 722, 726 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 726 EXCRETION FEW FECES 694, 701, 708, 715, 726 SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT 575, 582, 589, 596, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 617, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 624, 631, 638, 645, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 652, 659, 666, 673, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 680, 687 MASS(ES) SHOULDER-RIGHT WEEK 83 575, 582, 589, 596, 603, 610 569 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92553 M 63 APPEARANCE MALOCCLUSION SWOLLEN VENTRAL-ABDOMINAL THIN 295, 302, 309, 344, 365, 372, 379, 400, 407, 414, 421, 428, 435 435 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 428 435 DISCHARGE CLEAR ORAL <UNSPECIFIED> NASAL RED IN COLOR 386 190, 267, 274 RESPIRATION AUDIBLE IRREGULAR 211, 330, 365 197, 204, 211 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SCROTUM ROUGH HAIR COAT YELLOW HAIR COAT 407, 414, 421, 428, 330 29, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 204, 211, 218, 225, 309, 365, 372, 379, 435 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435 232, 239, 246, 267, 386, 393, 400 99, 106, 113, 120, 127 190, 197, 204, 211, 218 281, 288, 295, 302, 309 372, 379, 386, 393, 400 274, 281, 288, 295, 302 570 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92553 M 63 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92554 M 103 BEHAVIOR RECUMBENT STERNAL 719 DISCHARGE GENITAL RED IN COLOR 232 EXCRETION FEW FECES NO FECES 708, 715 719 EYES SQUINTED PERI-ORBITAL 719 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 715, 719 719 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 393, 400, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 571 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92554 M 103 NORMAL NO REMARKABLE OBSERVATIONS 666, 673, 680, 687, 694, 701 C92555 M 60 APPEARANCE HUNCHED 409, 414-415 EXCRETION NO FECES 409, 414-415 EYES RED DISCHARGE EYE-RIGHT EYES 409, 414-415 407 SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT 409, 414-415 407, 409, 414-415 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 379, 386 MASS(ES) VENTRAL-ABDOMINAL WEEK 54 372 572 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 31 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92556 M 63 APPEARANCE MALOCCLUSION THIN 407, 410, 414, 421, 428, 435 410, 414, 421, 428, 435 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 428, 435 EXCRETION FEW FECES 410, 414, 421, 428, 435 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400 C92557 M 89 APPEARANCE HUNCHED THIN 617 589, 596, 603, 610, 617 EXCRETION FEW FECES 617 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT 617 603, 610, 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 573 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 32 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92557 M 89 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582 C92558 M 80 APPEARANCE HUNCHED THIN 557 554, 557 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 557 EXCRETION FEW FECES 551, 554, 557 EYES SQUINTED PERI-ORBITAL 547, 551, 554, 557 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 574 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 33 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92559 D 79 SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL 477, 498, 505, 512, 519, 526, 533, 540, 547, 551 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) TAIL-PROXIMAL WEEK 72 VENTRAL-THORACIC WEEK 79 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 551C 547C, 551C C92560 M 80 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 560 RESPIRATION IRREGULAR 560 SKIN & PELAGE YELLOW HAIR COAT 560 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 575 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 34 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92560 M 80 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 MASS(ES) TAIL-PROXIMAL WEEK 79 547, 554, 560 C92561 M 63 BEHAVIOR RECUMBENT LATERAL 436 EXCRETION FEW FECES 436 RESPIRATION AUDIBLE 436 IRREGULAR 436 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 576 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 35 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92562 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY PATTIE MURPHY 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92563 M 84 APPEARANCE THIN 582, 584 EXCRETION FEW FECES NO FECES 581-582 584 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 577 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 36 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92564 M 70 APPEARANCE HEAD TILT RIGHT 487 THIN 487 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 487 EXCRETION FEW FECES 487 NONFORMED FECES 487 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92565 D 91 APPEARANCE LIMITED USE PAWS-HIND 631, 637 DISCHARGE NASAL RED IN COLOR 627, 631, 637 RESPIRATION LABORED 627, 631, 637 578 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 37 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92565 D 91 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624 C92566 M 23 APPEARANCE LIMITED USE LIMBS-ALL MALOCCLUSION 155 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155 BEHAVIOR ATAXIC <UNSPECIFIED> 155 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 C92567 D 91 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 633 579 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 38 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92568 D 101 SKIN & PELAGE SORE/SCAB DORSAL 694, 701 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 WART LIKE LESION(S) DORSAL WEEK 95 659, 666, 673, 680, 687, 694, 701 C92569 T 105 APPEARANCE THIN 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 610, 617, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 631, 638 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 93 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 580 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 39 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92569 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 93 722C, 729C, 731C C92570 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY KATHY EWERS 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 581 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 40 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92503 M1 498 72 TO 505 73 MASS(ES) TAIL-PROXIMAL WEEK 72 10 X 10MM 512 74 MASS(ES) TAIL-PROXIMAL WEEK 72 15 X 10MM 519 75 TO 561 81 MASS(ES) TAIL-PROXIMAL WEEK 72 20 X 15MM 568 82 TO 733 105 MASS(ES) TAIL-PROXIMAL WEEK 72 15 X 10MM 729 105 TO 733 105 MASS(ES) TAIL-PROXIMAL WEEK 105 15 X 10MM BOTTOM C92522 M1 435 63 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL WEEK 63 10 X 10MM 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 87 10 X 10MM 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL WEEK 87 15 X 15MM 582 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 41 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92522 M1 302 44 TO 316 46 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 10 X 10MM 323 47 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 20 X 15MM C92525 M1 477 69 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL WEEK 61 10 X 10MM C92533 M1 512 74 TO 526 76 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 10 X 10MM 533 77 TO 554 80 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 20 X 15MM 561 81 TO 568 82 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 20 X 20MM 575 83 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 25 X 25MM 582 84 TO 589 85 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 30 X 40MM 583 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 42 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92533 M1 512 74 TO 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 69 10 X 10MM 526 76 TO 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 69 15 X 10MM 547 79 MASS(ES) INGUINAL REGION-LEFT WEEK 69 20 X 15MM 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 69 20 X 20MM 561 81 TO 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 69 30 X 25MM 575 83 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 69 30 X 30MM 589 85 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 69 35 X 40MM C92537 M1 645 93 TO 666 96 MASS(ES) DORSAL WEEK 93 15 X 15MM 584 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 43 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92537 M1 631 91 TO 638 92 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 91 30 X 30MM 645 93 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 91 40 X 35MM 652 94 TO 666 96 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 91 45 X 50MM C92541 M1 470 68 TO 673 97 MASS(ES) DORSAL WEEK 68 10 X 10MM C92542 M1 715 103 TO 733 105 MASS(ES) DORSAL WEEK 103 10 X 10MM 645 93 TO 733 105 MASS(ES) TAIL-PROXIMAL WEEK 93 15 X 10MM C92544 M1 708 102 MASS(ES) LIMB-FRONT-LEFT WEEK 102 20 X 20MM 715 103 TO 732 105 MASS(ES) LIMB-FRONT-LEFT WEEK 102 30 X 30MM 585 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 44 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92551 M1 372 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 10 X 10MM C92559 M1 498 72 TO 505 73 MASS(ES) TAIL-PROXIMAL WEEK 72 10 X 10MM 512 74 TO 526 76 MASS(ES) TAIL-PROXIMAL WEEK 72 10 X 15MM 533 77 TO 551 79 MASS(ES) TAIL-PROXIMAL WEEK 72 25 X 20MM 547 79 TO 551 79 MASS(ES) VENTRAL-THORACIC WEEK 79 20 X 20MM C92569 M1 645 93 TO 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 93 10 X 10MM 586 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 45 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92571 M 101 APPEARANCE HEAD TILT LEFT MALOCCLUSION 694, 155, 246, 337, 428, 519, 610, 694, 701-702 162, 169, 253, 260, 344, 351, 435, 442, 526, 533, 617, 624, 701-702 176, 267, 358, 449, 540, 631, 183, 274, 365, 456, 547, 638, 190, 281, 372, 463, 554, 645, 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 659, 211, 302, 393, 484, 575, 666, 218, 309, 400, 491, 582, 673, 225, 232, 239 316, 323, 330 407, 414, 421 498, 505, 512 589, 596, 603 680-681, 687 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 687, 701-702 702 EXCRETION FEW FECES 681, 687, 694, 701-702 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 C92572 D 101 APPEARANCE BENT TAIL THIN 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610 701-702 587 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 46 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92572 D 101 EXCRETION FEW FECES 701-702 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 C92573 M 84 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-LEFT THIN 407, 414, 421, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 584 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 584 463, 470, 477 582, 584 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 584 EXCRETION FEW FECES 584 EYES RED DISCHARGE EYES 584 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 588 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 47 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92573 M 84 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 428, 435, 442 C92574 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92575 1 4 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL 15, 22 NORMAL NO REMARKABLE OBSERVATIONS 1, 8 C92576 M 97 APPEARANCE LIMITED USE LIMBS-HIND 673 SKIN & PELAGE SORE/SCAB DORSAL 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 589 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 48 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92576 M 97 MASS(ES) DORSAL WEEK 73 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C C92577 T 105 SKIN & PELAGE SORE/SCAB DORSAL 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 MASS(ES) AXILLARY REGION-LEFT WEEK 101 DORSAL WEEK 69 HEAD-MAXILLARY-LEFT WEEK 67 WEEK 105 701C, 708C, 715C, 722C, 729C, 733C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 733C 463, 470, 477, 484 729, 733 590 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 49 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92578 M 86 APPEARANCE THIN 589-590, 596, 598 BEHAVIOR ATAXIC <UNSPECIFIED> 598 EXCRETION FEW FECES 590, 596, 598 EYES RED DISCHARGE EYE-LEFT 598 SKIN & PELAGE PALE ENTIRE BODY 598 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 591 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 50 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92579 D 86 APPEARANCE THIN 589, 596, 602 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 602 COLD TO TOUCH ENTIRE BODY 602 EXCRETION FEW FECES 602 RESPIRATION LABORED 602 SKIN & PELAGE PALE ENTIRE BODY 582, 589, 596, 602 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL WEEK 86 596, 602 592 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 51 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92580 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 549 EXCRETION FEW FECES NONFORMED FECES 540, 547, 549 549 EYES RED DISCHARGE EYE-LEFT 549 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 449, 456, 463 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 526, 533 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 470, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 477, 484, 491, 498, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 505, 512, 519 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 421, 428, 435C, 442C 593 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 52 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92581 M 105 APPEARANCE HUNCHED OBES THIN 729 596 681, 687, 694, 701, 708, 715, 722, 729 EXCRETION FEW FECES NO FECES NONFORMED FECES 722 681, 687, 729 596 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB SHOULDER-RIGHT 722, 729 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 631, 638 MASS(ES) DORSAL WEEK 85 LIMB-HIND-RIGHT WEEK 93 SHOULDER-RIGHT WEEK 50 589C, 596C, 603C, 610C, 617C 645C, 652C, 659C, 666C, 673C, 680-681C, 687C, 694C, 701C, 708C 715C, 722C, 729C 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C 594 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92581 M 105 MASS(ES) SHOULDER-RIGHT WEEK 50 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C WART LIKE LESION(S) DORSAL WEEK 95 659, 666, 673, 680-681, 687, 694, 701, 708, 715, 722, 729 C92582 M 101 APPEARANCE LIMITED USE LIMB-FRONT-LEFT 701-702 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 701-702 EXCRETION FEW FECES 694, 701-702 SKIN & PELAGE SORE/SCAB SHOULDER-LEFT 694, 701-702 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 595 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 54 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92582 M 101 NORMAL NO REMARKABLE OBSERVATIONS 659, 666, 673 MASS(ES) SHOULDER-LEFT WEEK 98 680C, 687C, 694C, 701-702C C92583 T 105 SKIN & PELAGE SORE/SCAB DORSAL 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) DORSAL WEEK 74 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 596 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 55 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92584 M 60 APPEARANCE THIN 418 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 418 EXCRETION FEW FECES 414, 418 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407 C92585 M 39 APPEARANCE MALOCCLUSION MISSING TEETH 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267-268 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267-268 EXCRETION FEW FECES 253, 260, 267-268 RESPIRATION AUDIBLE LABORED 239 239, 246, 253, 260, 267-268 597 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 56 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92585 M 39 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15 MASS(ES) AXILLARY REGION-LEFT WEEK 39 AXILLARY REGION-RIGHT WEEK 37 VENTRAL-CERVICAL WEEK 37 267-268C 253, 260C, 267-268C 253C, 260C, 267-268C C92586 M 73 APPEARANCE THIN 503-506 EXCRETION FEW FECES 498, 503-506 SKIN & PELAGE YELLOW HAIR COAT 504-506 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 598 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 57 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92587 M 88 APPEARANCE THIN 589-590, 596, 603, 610 EXCRETION FEW FECES 590, 596, 603, 610 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92588 M 103 APPEARANCE MALOCCLUSION MISSING TEETH THIN 29, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 708, 715 99, 106, 113, 120, 127 190, 197, 204, 211, 218 281, 288, 295, 302, 309 372, 379, 386, 393, 400 463, 470, 477, 484, 491 554, 561, 568, 575, 582 645, 652, 659, 666, 673 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715 708, 715 599 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 58 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92588 M 103 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 708, 715 EXCRETION FEW FECES 708, 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92589 M 100 APPEARANCE THIN 687, 694 BEHAVIOR ATAXIC <UNSPECIFIED> 694 HYPOACTIVE <UNSPECIFIED> 694 EXCRETION FEW FECES 694 EYES RED DISCHARGE EYES 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 600 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 59 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92589 M 100 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680 C92590 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C92591 D 96 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 617, 624, 631, 638, 645 519, 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 533, 540 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 87 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 670 601 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 60 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92591 D 96 MASS(ES) VENTRAL-ABDOMINAL WEEK 79 547, 554, 561, 568, 575, 582C, 589C, 596C, 603C, 610C VENTRAL-ABDOMINAL-LEFT WEEK 95 659C, 666C, 670C VENTRAL-ABDOMINAL-RIGHT WEEK 72 498C, 505C, 512C C92592 M 88 APPEARANCE LIMITED USE LIMBS-HIND 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92593 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92594 D 96 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 602 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 61 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92594 D 96 NORMAL NO REMARKABLE OBSERVATIONS 659, 666-667 C92595 M 84 APPEARANCE THIN 582-583 EXCRETION FEW FECES 581-583 EYES RED DISCHARGE EYES 581-583 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL-THORACIC DORSAL-THORACIC-RIGHT 582-583 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 581-583 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 470, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 477, 484 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) DORSAL WEEK 73 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 581-583C 603 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 62 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92596 M 71 APPEARANCE THIN 477, 484, 491 EXCRETION FEW FECES 477, 484, 491 SKIN & PELAGE BROWN HAIR COAT SCROTUM 491 SORE/SCAB VENTRAL-CERVICAL 15 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 C92597 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 731 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 604 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 63 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92597 T 105 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610 C92598 T 105 DISCHARGE GENITAL RED IN COLOR 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 519, 589, 596, 603, 610, 680, 687, 694, 701, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 617, 624, 631, 638, 708, 722, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 554, 561, 568 645, 652, 659 MASS(ES) AXILLARY REGION-RIGHT WEEK 103 715C 605 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 64 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92599 D 55 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 385 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C92600 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92601 T 105 EXCRETION NONFORMED FECES 400 SKIN & PELAGE SORE/SCAB DORSAL 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 MASS(ES) DORSAL WEEK 70 484, 491, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C 606 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 65 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92601 T 105 WART LIKE LESION(S) DORSAL WEEK 104 722, 729, 733 C92602 T 105 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 596, 603, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 547, 554, 561, 568, 638, 645, 652, 659, 729, 733 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 575, 582, 589 666, 673, 680 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 505C, 512C, 519C, 526C C92603 M 19 APPEARANCE PARALYSIS LIMBS-HIND 127 BEHAVIOR COLD TO TOUCH LIMBS-HIND 127 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 607 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 66 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92603 M 19 NORMAL NO REMARKABLE OBSERVATIONS 113, 120 C92604 M 91 APPEARANCE THIN 589, 596, 603, 610, 617, 624, 631 EXCRETION FEW FECES 624, 631 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT 617, 624, 631 610, 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92605 D 104 BEHAVIOR COLD TO TOUCH ENTIRE BODY 722-723 RESPIRATION AUDIBLE 722-723 608 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 67 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92605 D 104 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 722-723 SORE/SCAB DORSAL 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722-723 VENTRAL-ABDOMINAL-RIGHT 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701, 708, 715, 722-723 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) DORSAL WEEK 79 VENTRAL-ABDOMINAL-LEFT WEEK 83 WEEK 87 VENTRAL-ABDOMINAL-RIGHT WEEK 75 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722-723 575C, 582C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722-723C 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 609 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 68 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92606 M 78 APPEARANCE LIMITED USE LIMBS-HIND 542 PAWS-FRONT 540 SWOLLEN MOUTH 141 BEHAVIOR RECUMBENT STERNAL 542 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE 491, 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 267, 274, 358, 365, 449 22, 29, 36, 43, 50, 127, 134, 148, 155, 281, 288, 295, 302, 372, 379, 386, 393, 57, 64, 71, 78, 85, 162, 169, 176, 183, 309, 316, 323, 330, 400, 407, 414, 421, 92, 99, 106 190, 197, 204 337, 344, 351 428, 435, 442 MASS(ES) SHOULDER-RIGHT WEEK 66 456, 463, 470, 477, 484, 491, 498, 505, 512C, 519C, 526C, 533C 540C, 542C WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 31 NOSE WEEK 75 211, 218, 225, 232, 239, 246, 253, 260 519, 526, 533, 540, 542 610 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 69 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92607 M 91 APPEARANCE THIN 589, 596, 603, 610, 617, 624, 631 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 617, 624, 631 204, 211, 218 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 239, 246, 330, 337, 421, 428, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 57, 64, 71, 78, 85, 155, 162, 169, 176, 281, 288, 295, 302, 372, 379, 386, 393, 463, 470, 477, 484, 554, 561, 568, 575, 92, 99, 106 183, 225, 232 309, 316, 323 400, 407, 414 491, 498, 505 582 MASS(ES) INGUINAL REGION-RIGHT WEEK 28 VENTRAL-ABDOMINAL-LEFT WEEK 30 190C, 197C 204, 211 C92608 M 79 APPEARANCE HUNCHED MALOCCLUSION THIN 540, 547-548 99, 101, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547-548 533, 540, 547-548 611 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 70 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92608 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 548 EXCRETION FEW FECES 512, 540, 547-548 RESPIRATION AUDIBLE LABORED 101, 106 99 SKIN & PELAGE ROUGH HAIR COAT 491, 498, 505, 512, 519, 526, 533, 540, 547-548 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 C92609 M 104 APPEARANCE LIMITED USE LIMBS-HIND 724 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 724 EYES CLEAR DISCHARGE EYE-LEFT EYES 589, 596, 603, 610, 724 659, 666, 673, 680, 687, 694, 701, 708 RESPIRATION LABORED 724 612 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 71 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92609 M 104 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 617, 624, 631, 638, 645, 652 MASS(ES) DORSAL WEEK 102 708C, 715C, 722C, 724C WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 98 680C, 687C, 694C, 701C, 708C, 715C, 722C, 724C C92610 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92611 M 88 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 449, 456, 463, 470, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 WEEK 72 428, 435, 442 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C 613 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 72 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92611 M 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 589C, 596C, 603C, 610C C92612 M 79 APPEARANCE THIN 533, 540, 547 BEHAVIOR HYPOACTIVE SEVERE 547 RECUMBENT LATERAL 547 COLD TO TOUCH ENTIRE BODY 547 RESPIRATION IRREGULAR 547 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT 169 533, 540, 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 57, 64, 71, 78, 85, 155, 162, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526 92, 99, 106 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 614 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 73 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92613 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92614 D 92 SKIN & PELAGE SORE/SCAB DORSAL DORSAL-CERVICAL VENTRAL-ABDOMINAL-LEFT 631, 638, 640 617, 624 323, 330 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 505, 512, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 365, 372, 379, 386, 456, 463, 470, 477, 547, 554, 561, 568, 92, 99, 106 183, 190, 197 274, 281, 288 393, 400, 407 484, 491, 498 575, 582, 589 MASS(ES) DORSAL WEEK 89 VENTRAL-ABDOMINAL WEEK 45 617C, 624C, 631C, 638C, 640C 309, 316 C92615 M 90 BEHAVIOR RECUMBENT LATERAL 624 EXCRETION FEW FECES 624 615 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 74 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92615 M 90 SKIN & PELAGE ROUGH HAIR COAT 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92616 2 14 EYES CLEAR DISCHARGE EYE-LEFT 50 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 57, 64, 71, 78, 85, 92-93 C92617 M 81 APPEARANCE THIN 561, 565 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 565 RECUMBENT LATERAL 565 SENSITIVE TO TOUCH 561 EXCRETION NO FECES 565 616 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 75 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92617 M 81 EYES PALE EYES 533, 540, 547, 554, 561, 565 RED DISCHARGE EYES 565 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92618 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92619 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 708, 715, 722, 729, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 554, 561, 568 645, 652, 659 732 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 442C 617 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 76 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92620 D 96 APPEARANCE MALOCCLUSION MISSING TEETH 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 99, 106, 113, 120, 127, 134, 141, 148, 155 190, 197, 204, 211, 218, 225, 232, 239, 246 281, 288, 295, 302, 309, 316, 323, 330, 337 372, 379, 386, 393, 400, 407, 414, 421, 428 463, 470, 477, 484, 491, 498, 505, 512, 519 554, 561, 568, 575, 582, 589, 596, 603, 610 645, 652, 659, 666-667 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666-667 EXCRETION FEW FECES 666-667 EYES RED DISCHARGE EYE-RIGHT SCAB EYE-RIGHT 561, 568 316 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-LEFT 561, 568 505, 512, 519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 491C, 498C 618 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 77 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92621 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92622 D 83 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 533, 540, 547, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 554, 561, 568, 575, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 578 MASS(ES) DORSAL WEEK 72 498, 505, 512, 519, 526 C92623 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 659, 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 715, 722, 729, 733 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 619 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 78 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92624 D 61 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 425 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C92625 M 84 APPEARANCE HUNCHED THIN TREMORS HEAD 588 581-582, 588 588 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 588 COLD TO TOUCH ENTIRE BODY 588 EXCRETION FEW FECES 581-582, 588 RESPIRATION IRREGULAR 588 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL 588 561, 568 620 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 79 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92625 M 84 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 575 C92626 T 105 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-LEFT 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 729, 732 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 EYES CLEAR DISCHARGE EYE-RIGHT 526 RED DISCHARGE EYE-RIGHT 533 621 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 80 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92626 T 105 EYES SCAB EYE-RIGHT 316 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 C92627 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92628 M 73 APPEARANCE THIN 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505-506 EXCRETION FEW FECES 372, 428, 435, 442 SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT 456, 463, 470, 477, 484, 491, 498, 505-506 372 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 379, 386 622 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 81 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92629 D 78 APPEARANCE THIN 540, 546 EXCRETION FEW FECES 540, 546 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92630 M 88 APPEARANCE THIN 589, 596, 603, 610, 616 BEHAVIOR ATAXIC <UNSPECIFIED> 616 HYPOACTIVE <UNSPECIFIED> 616 EYES CLEAR DISCHARGE EYES RED DISCHARGE EYES 603, 610 616 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 623 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 82 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92630 M 88 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 73 505, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 616C 624 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 83 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92576 M2 505 73 TO 512 74 MASS(ES) DORSAL WEEK 73 10 X 10MM 519 75 TO 540 78 MASS(ES) DORSAL WEEK 73 15 X 15MM 547 79 MASS(ES) DORSAL WEEK 73 20 X 15MM 554 80 TO 561 81 MASS(ES) DORSAL WEEK 73 20 X 20MM 568 82 TO 582 84 MASS(ES) DORSAL WEEK 73 20 X 25MM 589 85 TO 596 86 MASS(ES) DORSAL WEEK 73 20 X 30MM 603 87 TO 617 89 MASS(ES) DORSAL WEEK 73 25 X 30MM 624 90 TO 673 97 MASS(ES) DORSAL WEEK 73 30 X 40MM 625 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 84 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92577 M2 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 101 30 X 30MM 708 102 TO 733 105 MASS(ES) AXILLARY REGION-LEFT WEEK 101 30 X 40MM 477 69 TO 505 73 MASS(ES) DORSAL WEEK 69 10 X 10MM 512 74 TO 526 76 MASS(ES) DORSAL WEEK 69 15 X 15MM 533 77 TO 561 81 MASS(ES) DORSAL WEEK 69 20 X 20MM 568 82 TO 673 97 MASS(ES) DORSAL WEEK 69 20 X 25MM 680 98 TO 722 104 MASS(ES) DORSAL WEEK 69 25 X 25MM 729 105 TO 733 105 MASS(ES) DORSAL WEEK 69 30 X 30MM 626 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 85 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92580 M2 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 10 X 10MM 442 64 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 15 X 15MM C92581 M2 589 85 TO 617 89 MASS(ES) DORSAL WEEK 85 10 X 15MM 645 93 TO 729 105 MASS(ES) LIMB-HIND-RIGHT WEEK 93 10 X 10MM 344 50 TO 372 54 MASS(ES) SHOULDER-RIGHT WEEK 50 10 X 10MM 379 55 TO 386 56 MASS(ES) SHOULDER-RIGHT WEEK 50 15 X 15MM 393 57 TO 505 73 MASS(ES) SHOULDER-RIGHT WEEK 50 20 X 20MM 512 74 TO 547 79 MASS(ES) SHOULDER-RIGHT WEEK 50 15 X 15MM 627 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 86 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92582 M2 680 98 TO 687 99 MASS(ES) SHOULDER-LEFT WEEK 98 40 X 40MM 694 100 TO 702 101 MASS(ES) SHOULDER-LEFT WEEK 98 60 X 40MM C92583 M2 512 74 TO 673 97 MASS(ES) DORSAL WEEK 74 10 X 10MM 680 98 TO 731 105 MASS(ES) DORSAL WEEK 74 15 X 15MM C92585 M2 267 39 TO 268 39 MASS(ES) AXILLARY REGION-LEFT WEEK 39 10 X 10MM 260 38 TO 268 39 MASS(ES) AXILLARY REGION-RIGHT WEEK 37 20 X 20MM 253 37 TO 268 39 MASS(ES) VENTRAL-CERVICAL WEEK 37 30 X 20MM C92591 M2 582 84 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 79 10 X 10MM 628 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 87 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92591 M2 659 95 TO 670 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 95 10 X 10MM 498 72 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 25 X 25MM C92595 M2 505 73 MASS(ES) DORSAL WEEK 73 10 X 10MM 512 74 TO 526 76 MASS(ES) DORSAL WEEK 73 15 X 15MM 533 77 TO 561 81 MASS(ES) DORSAL WEEK 73 25 X 25MM 568 82 TO 583 84 MASS(ES) DORSAL WEEK 73 25 X 30MM C92598 M2 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 103 10 X 10MM C92601 M2 498 72 TO 715 103 MASS(ES) DORSAL WEEK 70 10 X 10MM 629 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 88 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92602 M2 505 73 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 10 X 10MM C92605 M2 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 30 X 30MM 603 87 TO 723 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 87 40 X 50MM C92606 M2 512 74 TO 542 78 MASS(ES) SHOULDER-RIGHT WEEK 66 10 X 10MM C92607 M2 190 28 TO 197 29 MASS(ES) INGUINAL REGION-RIGHT WEEK 28 10 X 10MM C92609 M2 708 102 TO 724 104 MASS(ES) DORSAL WEEK 102 10 X 10MM 680 98 TO 724 104 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 98 CORNER OF LEFT EYE C92611 M2 498 72 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 15 X 15MM 630 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 89 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92611 M2 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 20 X 20MM 519 75 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 25 X 25MM 540 78 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 45 X 40MM 554 80 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 50 X 50MM 568 82 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 60 X 60MM 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 75 X 80MM 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 80 X 80MM 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 100 X 100MM 631 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 90 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92611 M2 610 88 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 120 X 120MM C92614 M2 617 89 TO 631 91 MASS(ES) DORSAL WEEK 89 10 X 10MM 638 92 TO 640 92 MASS(ES) DORSAL WEEK 89 15 X 15MM C92619 M2 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 30 X 10MM C92620 M2 491 71 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM C92630 M2 512 74 TO 616 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 73 10 X 10MM 632 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 91 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92631 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92632 M 103 APPEARANCE THIN 715 BEHAVIOR COLD TO TOUCH ENTIRE BODY 715 EXCRETION FEW FECES 715 RESPIRATION LABORED 715 SKIN & PELAGE SORE/SCAB EAR-LEFT 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 633 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 92 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92632 M 103 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323 MASS(ES) EAR-LEFT WEEK 48 VENTRAL-CERVICAL-LEFT WEEK 103 330, 337, 344, 351, 358, 365, 421C, 428C, 435C, 442C, 449C, 498C, 505C, 512C, 519C, 526C, 575C, 582C, 589C, 596C, 603C, 652C, 659C, 666C, 673C, 680C, 372, 379, 386, 393, 400, 407, 456C, 463C, 470C, 477C, 484C, 533C, 540C, 547C, 554C, 561C, 610C, 617C, 624C, 631C, 638C, 687C, 694C, 701C, 708C, 715C 414 491C 568C 645C 715C C92633 T 105 APPEARANCE MALOCCLUSION 330, 337, 344, 351, 365, 372, 379, 386, 393, 400, 407, 428, 435 442, 449, 456, 463, 470, 477, 484, 540, 547, 554, 561, 568, 575 582, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 732 DISCHARGE NASAL RED IN COLOR 22 UNKNOWN RED IN COLOR 21 RESPIRATION AUDIBLE 21 634 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 93 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92633 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 533, 589, 29, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 358, 414, 596, 603, 610, 617 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 421, 491, 498, 505, 99, 106, 113 190, 197, 204 281, 288, 295 512, 519, 526 C92634 D 87 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92635 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 549 EXCRETION FEW FECES NONFORMED FECES 547, 549 549 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 519, 526, 533, 540, 547, 549 635 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 94 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92635 M 79 SKIN & PELAGE ROUGH HAIR COAT 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 477, 484, 491, 498, 505, 512 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 76 526C, 533C, 540C, 547C, 549C C92636 D 98 APPEARANCE SWOLLEN TESTES 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 682 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT 400 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 682 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302 636 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 95 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92636 D 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 88 610 C92637 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92638 M 91 APPEARANCE THIN 589, 596, 603, 610, 617, 624, 631 SKIN & PELAGE PALE ENTIRE BODY 610, 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 526, 533, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 540, 547, 554, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 505, 512, 519 637 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 96 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92639 M 85 APPEARANCE HUNCHED THIN 589 512, 519, 526, 533, 540, 547, 582, 589 EXCRETION FEW FECES 512, 589 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 512, 519, 526, 533, 540, 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505, 554, 561, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 568, 575 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 MASS(ES) TAIL WEEK 49 337 C92640 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 638 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 97 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92641 T 105 APPEARANCE MALOCCLUSION MISSING TEETH 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 358, 365, 372, 379 C92642 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92643 T 105 APPEARANCE MALOCCLUSION 554, 666, 673 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 372, 379 639 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 98 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92643 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 526, 533, 540, 547, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 680, 687, 694 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 73 VENTRAL-ABDOMINAL-RIGHT WEEK 101 VENTRAL-THORACIC-RIGHT WEEK 101 505, 512, 519 701C 708C, 715C, 722C, 729C, 733C C92644 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) DORSAL WEEK 104 722, 729, 731 640 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 99 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92645 2 14 EYES RED DISCHARGE EYE-LEFT 64 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 71, 78, 85, 92-93 C92646 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92647 M 80 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 557 EXCRETION FEW FECES 551 NO FECES 557 EYES SQUINTED PERI-ORBITAL 557 SKIN & PELAGE ROUGH HAIR COAT 554, 557 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 641 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 100 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92648 M 87 APPEARANCE THIN 589, 596, 599, 603, 605 BEHAVIOR AGGRESSIVE <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 589, 596 605 EXCRETION FEW FECES 605 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 463, 470 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 526, 533, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 540, 547, 554, 561, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 477, 484, 491, 498, 568, 575, 582 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 505, 512, 519 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 428, 435, 442C, 449C, 456C 642 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 101 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92649 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-RIGHT 722 428, 435, 442, 624, 631, 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 498, 505, 589, 596, 701, 708, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 512, 519, 526, 533, 603, 610, 617, 645, 715, 729, 732 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 449, 456, 463, 470, 540, 547, 554, 561, 652, 659, 666, 673, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 568, 575, 582 680, 687, 694 C92650 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92651 M 81 APPEARANCE SWOLLEN PAW-HIND-RIGHT TAIL-PROXIMAL THIN 533, 540, 547, 554, 561 540, 547, 554, 561 547, 554, 561 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 561 EXCRETION FEW FECES 561 643 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 102 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92651 M 81 EYES SQUINTED PERI-ORBITAL 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92652 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92653 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 610, 617, 624, 631, 638, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 701, 708, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 715, 722, 729, 733 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 652, 659, 666, 673, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 680, 687, 694 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 610C, 617C 644 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 103 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92654 M 79 EXCRETION NO FECES 549 EYES CLEAR DISCHARGE EYE-LEFT OPAQUE EYE-LEFT PROTRUDING EYE-LEFT SCAB EYE-LEFT 519 526, 533, 540, 547, 549 549 533, 540, 547, 549 SKIN & PELAGE SORE/SCAB HEAD-CRANIAL 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 645 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 104 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92655 M 94 SKIN & PELAGE SORE/SCAB TAIL-MID 624, 631, 638, 645, 652-653 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) TAIL-MID WEEK 85 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652-653C C92656 M 97 APPEARANCE THIN 659, 666, 673 EXCRETION FEW FECES 645, 652, 673 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL-THORACIC 673 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 646 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92656 M 97 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 MASS(ES) DORSAL WEEK 49 337, 344, 351, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C C92657 T 105 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-LEFT 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 617 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 561, 568, 575, 582, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 589, 603, 610 MASS(ES) DORSAL WEEK 93 HEAD-MAXILLARY-RIGHT WEEK 91 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C 631, 638, 645 647 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 106 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92657 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 519, 526, 533, 540, 547, 554 WART LIKE LESION(S) SHOULDER-LEFT WEEK 90 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 729, 733 C92658 D 94 EYES RED DISCHARGE EYE-RIGHT 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 603, 610, 617 624, 631, 638, 645, 652-653 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 554, 561, 568, 575, 582, 589, 596 C92659 T 105 APPEARANCE MALOCCLUSION SWOLLEN PERI-ORAL PAW-HIND-LEFT 407, 456, 463, 554, 561, 568, 575, 582, 589, 596, 603, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 652, 659, 666 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 648 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 107 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92659 T 105 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 414, 421, 428, 435, 442, 449, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547 C92660 T 105 APPEARANCE MALOCCLUSION 463, 470, 477, 484, 533, 561, 568, 575, 582, 589, 596, 603, 610 659, 666, 673, 680, 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 505, 512, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 519, 526, 540, 547, 701, 715, 722, 729, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 554, 617, 624, 631, 732 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 491, 498 638, 645, 652 C92661 M 90 APPEARANCE THIN 589, 596, 603, 610, 617, 624 EXCRETION NO FECES 589 SKIN & PELAGE PALE ENTIRE BODY 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 649 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 108 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92661 M 90 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 WART LIKE LESION(S) VENTRAL-THORACIC-LEFT WEEK 46 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 C92662 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92663 D 95 DISCHARGE GENITAL RED IN COLOR 379 EXCRETION DISCOLORED URINE RED IN COLOR 400, 498, 504 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 407, 491, 505, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 414, 421, 428, 435, 512, 519, 526, 533, 603, 610 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 442, 449, 456, 463, 540, 547, 554, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 386 470, 477, 484 568, 575, 582 650 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 109 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92663 D 95 MASS(ES) SHOULDER-LEFT WEEK 89 617C, 624C, 631C, 638C, 645C, 652C, 659-660C C92664 D 78 APPEARANCE THIN 533, 540 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 540 RECUMBENT LATERAL 540 RESPIRATION LABORED 540 SKIN & PELAGE ROUGH HAIR COAT 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 651 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 110 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92665 D 88 APPEARANCE SWOLLEN PAW-HIND-RIGHT 575, 582, 589, 596, 603, 610, 614 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92666 M 76 APPEARANCE HEAD TILT RIGHT THIN 526, 530 512, 519, 526, 530 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 530 EXCRETION FEW FECES 512, 530 SKIN & PELAGE ROUGH HAIR COAT 526, 530 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 652 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 111 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92666 M 76 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 C92667 M 79 APPEARANCE THIN 547, 549 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 549 EXCRETION FEW FECES 526, 528, 533, 540, 547, 549 EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES 43 512, 519 526, 528, 533, 540 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 528, 533, 540, 547, 549 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 22, 29, 36, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470 653 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 112 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92667 M 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 528C, 533C, 540C 547C, 549C C92668 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92669 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C92670 M 74 APPEARANCE HUNCHED THIN 512 498, 505, 512 EXCRETION NONFORMED FECES 456 SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT SORE/SCAB LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT 512 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 449, 456, 463, 470, 477 449, 456, 463, 470, 477, 484 654 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 113 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92670 M 74 SKIN & PELAGE SORE/SCAB LATERAL-CERVICAL-RIGHT 484, 491, 498, 505 SHOULDER-LEFT 449, 456 SHOULDER-RIGHT 449, 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C92671 D 93 APPEARANCE MALOCCLUSION 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 650 DISCHARGE UNKNOWN RED IN COLOR 45 EYES RED DISCHARGE EYE-LEFT 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 218, 225 655 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 114 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92671 D 93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 MASS(ES) VENTRAL-ABDOMINAL WEEK 29 197C, 204C, 211C C92672 M 95 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 665 EXCRETION FEW FECES 656, 659, 665 EYES RED DISCHARGE EYE-LEFT 665 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 95 659, 665 656 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 115 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92673 D 95 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 505, 512, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 519, 526, 533, 540, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 477, 547, 554 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 484, 491, 498 MASS(ES) AXILLARY REGION-LEFT WEEK 81 561, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C WART LIKE LESION(S) LIMB-FRONT-RIGHT WEEK 65 449, 456, 463, 470 C92674 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 400, 407 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 505, 512, 596, 603, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 456, 463, 470, 477, 547, 554, 561, 568, 638, 645, 652, 659, 729, 731 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 484, 491, 498 575, 582, 589 666, 673, 680 657 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 116 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92674 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 56 386C, 393C C92675 M 102 APPEARANCE THIN 708, 712 EYES RED DISCHARGE EYE-LEFT 680, 687, 694, 701, 708, 712 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) DORSAL WEEK 90 624, 631, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 712C 658 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 117 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92676 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92677 D 99 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 WART LIKE LESION(S) DORSAL WEEK 80 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 690 C92678 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92679 M 55 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 383 EXCRETION FEW FECES 383 EYES RED DISCHARGE EYES 383 RESPIRATION IRREGULAR 383 659 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 118 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92679 M 55 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 C92680 M 84 APPEARANCE HUNCHED THIN 582-583 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582-583 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 491, 498 EXCRETION FEW FECES 469-470, 477, 498, 547, 575, 582-583 EYES SQUINTED PERI-ORBITAL 561, 568, 575, 582-583 SKIN & PELAGE PALE ENTIRE BODY 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582-583 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 660 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 119 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92680 M 84 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 76 526, 533, 540, 547 C92681 M 87 APPEARANCE THIN 596, 603, 606 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 606 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) TAIL-PROXIMAL WEEK 62 428, 435, 442, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 606C 661 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 120 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92682 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92683 T 105 APPEARANCE SWOLLEN LIMB-HIND-LEFT 715, 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 662 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 121 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92684 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92685 M 80 APPEARANCE MALOCCLUSION THIN 71, 78, 232, 239 547, 554 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 554 RECUMBENT LATERAL 554 DISCHARGE GENITAL RED IN COLOR 113 EXCRETION FEW FECES 547, 554 EYES RED DISCHARGE EYES 554 SQUINTED PERI-ORBITAL-RIGHT 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 85, 92, 99, 106, 120, 127 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 663 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 122 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92685 M 80 NORMAL NO REMARKABLE OBSERVATIONS 512, 519, 526, 533, 540 SACRIFICE SACRIFICE APPROVED BY TOXICOLOGIST 554 C92686 M 84 APPEARANCE MALOCCLUSION 554, 561, 568, 575, 582 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 582 EXCRETION FEW FECES 575, 582 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 582 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 664 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 123 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92687 M 72 APPEARANCE THIN 477, 484, 491, 498, 502 EXCRETION FEW FECES 469-470, 477, 484, 491, 498, 502 EYES DILATED PUPIL EYES 470, 502 SKIN & PELAGE ROUGH HAIR COAT 470, 477, 484, 491, 498, 502 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 19 WEEK 57 127, 134, 141, 148, 155, 162, 169, 176 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 469-470 477, 484, 491, 498, 502 665 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 124 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92688 M 97 APPEARANCE SWOLLEN LIMB-HIND-LEFT PAW-HIND-LEFT THIN 498, 505, 512, 519 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 677 652, 659, 666, 673, 677 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 677 EXCRETION FEW FECES 677 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92689 D 56 APPEARANCE MALOCCLUSION 386, 390 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 666 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 125 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92690 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 667 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 126 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92632 M3 421 61 TO 428 62 MASS(ES) EAR-LEFT WEEK 48 10 X 10MM 435 63 TO 442 64 MASS(ES) EAR-LEFT WEEK 48 15 X 10MM 449 65 TO 484 70 MASS(ES) EAR-LEFT WEEK 48 15 X 15MM 491 71 MASS(ES) EAR-LEFT WEEK 48 25 X 20MM 498 72 TO 512 74 MASS(ES) EAR-LEFT WEEK 48 10 X 10MM 519 75 TO 610 88 MASS(ES) EAR-LEFT WEEK 48 15 X 15MM 617 89 TO 708 102 MASS(ES) EAR-LEFT WEEK 48 20 X 20MM 715 103 TO 715 103 MASS(ES) EAR-LEFT WEEK 48 30 X 30MM 668 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 127 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92632 M3 715 103 TO 715 103 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 103 25 X 45MM C92635 M3 526 76 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 76 15 X 15MM 533 77 TO 549 79 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 76 20 X 15MM C92643 M3 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 101 40 X 40MM 708 102 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 101 40 X 50MM 715 103 TO 733 105 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 101 60 X 60MM C92648 M3 442 64 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 10 X 10MM C92653 M3 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 10 X 10MM 669 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 128 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92653 M3 617 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 20 X 20MM C92655 M3 589 85 TO 596 86 MASS(ES) TAIL-MID WEEK 85 10 X 10MM 603 87 TO 631 91 MASS(ES) TAIL-MID WEEK 85 20 X 15 638 92 TO 645 93 MASS(ES) TAIL-MID WEEK 85 20 X 20MM 652 94 TO 653 94 MASS(ES) TAIL-MID WEEK 85 20 X 25MM C92656 M3 358 52 TO 365 53 MASS(ES) DORSAL WEEK 49 10 X 10MM 372 54 TO 407 59 MASS(ES) DORSAL WEEK 49 15 X 15MM 414 60 MASS(ES) DORSAL WEEK 49 30 X 30MM 670 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 129 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92656 M3 421 61 TO 484 70 MASS(ES) DORSAL WEEK 49 20 X 20MM 491 71 TO 554 80 MASS(ES) DORSAL WEEK 49 30 X 30MM 561 81 MASS(ES) DORSAL WEEK 49 30 X 20MM 568 82 TO 582 84 MASS(ES) DORSAL WEEK 49 10 X 15MM C92657 M3 645 93 TO 722 104 MASS(ES) DORSAL WEEK 93 10 X 10MM C92663 M3 617 89 TO 624 90 MASS(ES) SHOULDER-LEFT WEEK 89 25 X 30MM 631 91 MASS(ES) SHOULDER-LEFT WEEK 89 35 X 40MM 638 92 MASS(ES) SHOULDER-LEFT WEEK 89 50 X 60MM 671 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 130 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92663 M3 645 93 MASS(ES) SHOULDER-LEFT WEEK 89 70 X 70MM 652 94 TO 660 95 MASS(ES) SHOULDER-LEFT WEEK 89 70 X 80MM C92667 M3 477 69 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 505 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 528 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 672 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 131 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92667 M3 528 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 533 77 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 549 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM 549 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 30 X 30MM C92671 M3 197 29 TO 211 31 MASS(ES) VENTRAL-ABDOMINAL WEEK 29 10 X 10MM C92673 M3 568 82 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 81 15 X 20MM 673 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 132 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92673 M3 582 84 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 81 20 X 25MM 603 87 TO 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 81 30 X 40MM 617 89 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 81 40 X 40MM 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 81 40 X 50MM 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 81 50 X 50MM 645 93 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 81 50 X 60MM C92674 M3 386 56 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 56 10 X 10MM C92675 M3 638 92 TO 652 94 MASS(ES) DORSAL WEEK 90 10 X 10MM 674 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 133 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92675 M3 659 95 TO 712 102 MASS(ES) DORSAL WEEK 90 15 X 15MM C92681 M3 449 65 TO 606 87 MASS(ES) TAIL-PROXIMAL WEEK 62 20 X 10MM 675 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 134 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92691 T 105 APPEARANCE MALOCCLUSION 589, 659, 666, 673, 708, 715 EYES DISCOLORED/LIGHT RED EYE-RIGHT 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 596, 603, 610 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92692 D 53 APPEARANCE MALOCCLUSION THIN 148, 155, 309, 316, 323, 330, 337, 344, 351, 358, 365 358, 365 EXCRETION FEW FECES 358, 365 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 676 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 135 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92693 T 105 EYES RED DISCHARGE EYE-LEFT EYE-RIGHT 302, 400, 596 533 SKIN & PELAGE BLACK SKIN DORSAL SORE/SCAB DORSAL 687, 694, 701, 708, 715, 722, 729, 733 680, 687, 694, 701, 708, 715, 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 323, 330, 421, 428, 526, 540, 631, 638, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 337, 344, 351, 358, 435, 442, 449, 456, 547, 554, 561, 568, 645, 652, 659, 666, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 365, 372, 379, 386, 463, 470, 477, 484, 575, 582, 589, 603, 673 92, 99, 106 183, 190, 197 295, 309, 316 393, 407, 414 491, 512, 519 610, 617, 624 MASS(ES) DORSAL WEEK 98 VENTRAL-ABDOMINAL WEEK 72 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 498C, 505C WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 40 274, 281, 288 677 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 136 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92694 T 105 APPEARANCE MALOCCLUSION 463, 526, 554, 561, 568, 575, 582, 589, 659, 708, 715 EYES RED DISCHARGE EYE-RIGHT 652 SKIN & PELAGE SORE/SCAB MOUTH PAW-HIND-RIGHT 463, 470, 477, 603, 610, 617 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 519, 533, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 540, 547, 596, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 484, 491, 631, 638, 645, 694, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 498, 505, 512 701 MASS(ES) MOUTH WEEK 64 VENTRAL-ABDOMINAL-RIGHT WEEK 95 442C, 449C, 456C 659C, 666C, 673C, 680C, 687C 678 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 137 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92695 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92696 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92697 M 67 APPEARANCE THIN 463, 465 BEHAVIOR RECUMBENT LATERAL 465 679 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 138 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92697 M 67 EXCRETION NO FECES 465 EYES RED DISCHARGE EYES 465 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC 414, 421, 428, 435, 442, 449, 456, 463, 465 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393 MASS(ES) DORSAL WEEK 58 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 465C C92698 T 105 EXCRETION FEW FECES LIQUID FECES 729, 732 729, 732 EYES PALE EYES 729, 732 680 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 139 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92698 T 105 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 729, 732 SORE/SCAB AXILLARY REGION-LEFT 687, 694, 701, 708, 715, 722, 729, 732 OTHER 729C, 732C NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 WART LIKE LESION(S) AXILLARY REGION-LEFT WEEK 82 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 C92699 T 105 APPEARANCE MALOCCLUSION 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 681 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 140 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92700 T 105 APPEARANCE MALOCCLUSION SWOLLEN VENTRAL-ABDOMINAL 554, 561, 568, 575 323, 330, 414, 421, 428, 435, 442, 449, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 733 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 722 EXCRETION NONFORMED FECES 288 SKIN & PELAGE ROUGH HAIR COAT 78, 85, 92, 99, 106, 120, 127, 134, 141, 148, 155, 162, 169, 176 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680, 687 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 113, 274, 281 MASS(ES) AXILLARY REGION-LEFT WEEK 98 VENTRAL-ABDOMINAL-LEFT WEEK 58 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 400C 682 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 141 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92701 M 53 APPEARANCE HUNCHED 365 THIN 365 BEHAVIOR COLD TO TOUCH ENTIRE BODY 365 EXCRETION FEW FECES 365 EYES RED DISCHARGE EYE-RIGHT 365 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB LIMB-FRONT-RIGHT 365 351, 358, 365 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 323C, 330C, 337C, 344C, 351C, 358C, 365C 683 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 142 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92702 T 105 APPEARANCE HEAD TILT LEFT 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92703 D 61 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 423 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C92704 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 684 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 143 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92704 T 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 90 624, 631, 638, 645, 652, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 715C, 722C, 729C, 732C C92705 T 105 EYES DISCOLORED/LIGHT RED EYE-RIGHT 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92706 M 98 APPEARANCE LIMITED USE LIMBS-HIND 680 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 680 EYES RED DISCHARGE EYES 680 685 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 144 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92706 M 98 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 575, 582 VENTRAL-ABDOMINAL-RIGHT 596, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 617, 624, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 631, 638, 645, 652, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 659, 666, 673 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 82 568C 568C, 575C, 582C, 589C, 596C C92707 M 90 APPEARANCE THIN 617, 624, 627 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 624, 627 DISCHARGE NASAL RED IN COLOR 624, 627 686 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 145 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92707 M 90 EXCRETION FEW FECES 624 EYES SQUINTED PERI-ORBITAL 624, 627 SKIN & PELAGE ROUGH HAIR COAT 617, 624, 627 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92708 T 105 APPEARANCE MALOCCLUSION 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 731 EYES CLEAR DISCHARGE EYE-RIGHT 540 687 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 146 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92708 T 105 EYES RED DISCHARGE EYE-RIGHT 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29 C92709 D 91 APPEARANCE MALOCCLUSION THIN 274, 281, 288, 330, 337, 344, 442, 463, 484, 491, 498, 505, 512 519, 575, 582, 589 617, 624, 631, 633 DISCHARGE NASAL RED IN COLOR 596 SKIN & PELAGE PALE ENTIRE BODY 631, 633 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 316, 323, 428, 435, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 351, 358, 365, 372, 449, 456, 470, 477, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 379, 386, 393, 400, 526, 554, 561, 568, 92, 99, 106 183, 190, 197 295, 302, 309 407, 414, 421 603, 610 MASS(ES) PAW-HIND-RIGHT WEEK 77 533, 540, 547 688 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 147 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92710 D 70 SKIN & PELAGE SORE/SCAB LATERAL-LEFT 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 463, 470, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 477, 484, 486 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 449, 456 MASS(ES) DORSAL WEEK 54 372, 379, 386, 393, 400, 407, 414, 421, 428C, 435C C92711 D 92 DISCHARGE GENITAL RED IN COLOR 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 617, 631, 638, 640 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 554, 561, 568 WART LIKE LESION(S) DORSAL-CERVICAL-LEFT WEEK 90 624 689 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 148 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92712 M 87 APPEARANCE THIN 596, 603 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 603 EXCRETION FEW FECES 581-582, 589, 596, 603 EYES SQUINTED PERI-ORBITAL 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92713 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 690 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 149 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92714 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92715 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92716 T 105 APPEARANCE THIN 411, 414, 421, 428, 435, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 EXCRETION FEW FECES NONFORMED FECES 411, 414 519, 729, 732 EYES DISCOLORED/LIGHT RED EYES 386, 393, 400, 407, 411, 414, 421, 428, 435, 442, 449, 456, 463 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA ROUGH HAIR COAT 414 414, 421 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 691 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 150 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92716 T 105 MASS(ES) MOUTH WEEK 83 575C, 582C WEEK 95 659, 666 C92717 T 105 SKIN & PELAGE SORE/SCAB DORSAL 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) DORSAL WEEK 98 VENTRAL-THORACIC-LEFT WEEK 86 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729, 733 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 692 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 151 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92718 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92719 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92720 D 83 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 575-576 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575-576C C92721 M 80 APPEARANCE MALOCCLUSION MISSING TEETH 29, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 99, 106, 113, 120, 127 190, 197, 204, 211, 218 281, 288, 295, 302, 309 372, 379, 386, 393, 400 463, 470, 477, 484, 491 554-555, 557 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 693 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 152 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92721 M 80 APPEARANCE MISSING TEETH 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554-555, 557 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 557 SENSITIVE TO TOUCH 557 EXCRETION FEW FECES NONFORMED FECES 555, 557 557 EYES SQUINTED PERI-ORBITAL-RIGHT 557 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 557 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 694 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 153 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92722 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92723 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 552 EXCRETION FEW FECES 551-552 EYES RED DISCHARGE EYES 552 SQUINTED PERI-ORBITAL-RIGHT 552 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 552 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 695 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 154 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92724 T 105 SKIN & PELAGE SORE/SCAB DORSAL VENTRAL-ABDOMINAL 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) DORSAL WEEK 74 INGUINAL REGION-RIGHT WEEK 86 VENTRAL-ABDOMINAL WEEK 73 512, 519, 526, 533, 540, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C 596C, 603C, 610C, 617C, 624C 505, 512, 519, 526, 533, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 731C WART LIKE LESION(S) DORSAL WEEK 96 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 696 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 155 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92725 D 52 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 363 SENSITIVE TO TOUCH 363 EXCRETION FEW FECES 363 EYES RED DISCHARGE EYES 363 RESPIRATION AUDIBLE 363 LABORED 363 SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT 363 HEAD-MAXILLARY-RIGHT 363 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 697 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 156 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92726 T 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT 659, 666, 673, 680 EXCRETION FEW FECES 624 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 694, 701, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 708, 715, 722, 729, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 631, 638, 733 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 645, 652, 687 C92727 D 90 DISCHARGE GENITAL RED IN COLOR 64 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 630 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 71, 78, 85, 92, 99, 106, 113 698 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 157 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92727 D 90 NORMAL NO REMARKABLE OBSERVATIONS 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533 C92728 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92729 M 79 APPEARANCE THIN 547, 549 EXCRETION FEW FECES NO FECES 540, 547 549 EYES RED DISCHARGE EYE-RIGHT EYES 540, 547 549 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 505, 512, 519, 526, 533, 540, 547, 549 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 699 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 158 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92729 M 79 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498 MASS(ES) INGUINAL REGION-LEFT WEEK 77 533C, 540C, 547C, 549C C92730 T 105 BEHAVIOR ATAXIC <UNSPECIFIED> 729, 732 EXCRETION FEW FECES 715, 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 105 729, 732 700 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 159 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92731 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92732 D 83 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 578 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92733 M 89 APPEARANCE THIN 589-590, 596, 603, 610, 614, 617 EXCRETION FEW FECES NO FECES 590, 596 614, 617 SKIN & PELAGE RED HAIR COAT LATERAL-ABDOMINAL-RIGHT 617 ROUGH HAIR COAT 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 701 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 160 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92734 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92735 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 197 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 225, 232, 316, 323, 407, 414, 498, 505, 589, 596, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 267, 274, 281, 288, 358, 365, 372, 379, 449, 456, 463, 470, 540, 547, 554, 561, 631, 638, 645, 652, 722, 729, 731 92, 99, 106 204, 211, 218 295, 302, 309 386, 393, 400 477, 484, 491 568, 575, 582 659, 666, 673 MASS(ES) VENTRAL-ABDOMINAL WEEK 27 183C, 190C C92736 T 105 DISCHARGE CLEAR ORAL MILD 15 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 22, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 29, 36, 43, 50, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 702 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 161 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92736 T 105 NORMAL NO REMARKABLE OBSERVATIONS 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 733 C92737 M 91 APPEARANCE CONVULSIONS 637 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 637 EXCRETION FEW FECES NO FECES 624, 631 637 RESPIRATION IRREGULAR 637 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) DORSAL WEEK 78 540, 547, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C, 631C, 637C 703 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 162 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92738 D 92 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 641 C92739 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 733 C92740 T 105 APPEARANCE MALOCCLUSION 225, 232, 239, 246, 253, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 666, 673, 680, 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 704 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 163 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92740 T 105 NORMAL NO REMARKABLE OBSERVATIONS 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 701, 708, 715, 722 729, 733 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 93 645C, 652C, 659C C92741 M 91 APPEARANCE HUNCHED THIN 633 617, 624, 631, 633 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL COLD TO TOUCH ENTIRE BODY 624, 631, 633 633 633 EXCRETION FEW FECES 624 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND YELLOW HAIR COAT 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 633 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 705 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 164 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92741 M 91 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533 C92742 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 610, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 414, 421, 428, 435, 442 449 MASS(ES) DORSAL WEEK 51 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C WART LIKE LESION(S) DORSAL WEEK 66 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 732 706 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 165 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92743 D 84 APPEARANCE OBES SWOLLEN VENTRAL-ABDOMINAL 582, 587 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 554, 561, 568 C92744 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92745 M 90 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL 456 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 624 EXCRETION FEW FECES 596 EYES PALE EYES 596, 603 707 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 166 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92745 M 90 SKIN & PELAGE PALE ENTIRE BODY 610, 617, 624 SORE/SCAB DORSAL 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 PAWS-HIND 596, 603, 610, 617, 624 VENTRAL-ABDOMINAL-LEFT 344 VENTRAL-ABDOMINAL-RIGHT 561, 568 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 463, 470, 477 MASS(ES) DORSAL WEEK 74 INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL-LEFT WEEK 46 VENTRAL-ABDOMINAL-RIGHT WEEK 70 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 582, 589, 596C, 603C, 610C, 617C, 624C 316C, 323C, 330C, 337C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 708 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 167 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92746 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 551 COLD TO TOUCH ENTIRE BODY 551 EXCRETION NO FECES 551 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 C92747 T 105 APPEARANCE SWOLLEN PAW-FRONT-RIGHT PAW-HIND-RIGHT 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 732 EYES RED DISCHARGE EYE-RIGHT 134, 141, 148, 155, 162, 169, 232, 239, 267, 274, 281, 288, 309 316, 323, 330, 337, 344, 358, 365, 372, 379, 386, 393, 400, 407 414, 435, 442, 449, 456, 463, 470, 519, 526, 533, 554, 561, 582 645, 652, 659, 680, 687, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 709 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 168 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92747 T 105 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 176, 183, 190, 197, 204, 211, 218, 225, 246, 253 260, 295, 302, 351, 421, 428, 477, 484, 491, 498, 505, 512, 540 547, 568, 575, 589, 596 C92748 T 105 SKIN & PELAGE SORE/SCAB LIMB-FRONT-RIGHT 372, 379, 386, 393, 400, 407, 414 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 526, 533, 617, 624, 708, 715, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 540, 547, 554, 561, 631, 638, 645, 652, 722, 729, 732 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 491, 498, 568, 575, 582, 589, 659, 666, 673, 680, 92, 99, 106 183, 190, 197 274, 281, 288 505, 512, 519 596, 603, 610 687, 694, 701 MASS(ES) LIMB-FRONT-RIGHT WEEK 51 351, 358, 365 WART LIKE LESION(S) LIMB-FRONT-RIGHT WEEK 61 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 710 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 169 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92749 D 103 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 435, 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 603, 610, 694, 701, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 617, 624, 631, 638, 708, 715, 718 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 449, 456, 575, 645, 652, 659, 666, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 582, 589, 596 673, 680, 687 WART LIKE LESION(S) TAIL-MID WEEK 67 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568 C92750 T 105 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 631, 638, 645, 652, 722, 729, 732 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 659, 666, 673 711 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 170 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92750 T 105 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 88 610, 617 712 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 171 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92693 M4 680 98 TO 687 99 MASS(ES) DORSAL WEEK 98 15 X 15MM 694 100 TO 708 102 MASS(ES) DORSAL WEEK 98 20 X 15MM 715 103 MASS(ES) DORSAL WEEK 98 20 X 25MM 722 104 MASS(ES) DORSAL WEEK 98 30 X30MM 729 105 TO 733 105 MASS(ES) DORSAL WEEK 98 40 X 40MM 498 72 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL WEEK 72 15 X 15MM C92694 M4 442 64 TO 456 66 MASS(ES) MOUTH WEEK 64 10 X 10MM 659 95 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 95 10 X 10MM 713 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 172 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92697 M4 400 58 TO 465 67 MASS(ES) DORSAL WEEK 58 15 X 15MM C92698 M4 729 105 SKIN & PELAGE TO 732 105 OTHER BLACK HAIR COAT - PERINEAL C92700 M4 680 98 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 98 10 X 10MM 729 105 TO 733 105 MASS(ES) AXILLARY REGION-LEFT WEEK 98 15 X 15MM 400 58 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 30 X 20MM C92701 M4 323 47 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 25 X 25MM 330 48 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 35 X 40MM 337 49 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 45 X 40 MM 714 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 173 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92701 M4 344 50 TO 351 51 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 65 X 60 MM 358 52 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 65 X 75MM 365 53 TO 365 53 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 70 X 80MM C92704 M4 659 95 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 90 10 X 10MM 680 98 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 90 15 X 15MM 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 90 20 X 20MM C92706 M4 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 10 X 10MM 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 10 X 10MM 715 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 174 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92706 M4 575 83 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 20 X 20MM C92710 M4 428 62 TO 435 63 MASS(ES) DORSAL WEEK 54 10 X 10MM C92716 M4 575 83 TO 582 84 MASS(ES) MOUTH WEEK 83 10 X 10MM C92717 M4 680 98 TO 722 104 MASS(ES) DORSAL WEEK 98 10 X 10MM 596 86 TO 617 89 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 86 10 X 10MM 624 90 TO 666 96 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 86 15 X 15MM 673 97 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 86 20 X 20MM 680 98 TO 694 100 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 86 25 X 25MM 716 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 175 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92717 M4 701 101 TO 733 105 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 86 30 X 30MM C92720 M4 526 76 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 10 X 10MM 533 77 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 30 X 30MM 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 40 X 40MM 547 79 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 60 X 60MM 554 80 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 80 X 60MM 561 81 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 110 X 110MM 568 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 120 X 110MM 717 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 176 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92720 M4 575 83 TO 576 83 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 140 X 130MM C92724 M4 547 79 TO 554 80 MASS(ES) DORSAL WEEK 74 10 X 10MM 561 81 TO 603 87 MASS(ES) DORSAL WEEK 74 20 X 20MM 610 88 TO 624 90 MASS(ES) DORSAL WEEK 74 10 X 10MM 596 86 TO 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 86 10 X 10MM 610 88 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 86 15 X 15MM 540 78 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 73 10 X 10MM 575 83 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL WEEK 73 20 X 20MM 718 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 177 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92724 M4 596 86 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL WEEK 73 30 X 30MM 610 88 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 73 15 X 15MM 666 96 TO 694 100 MASS(ES) VENTRAL-ABDOMINAL WEEK 73 20 X 20MM 701 101 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 73 10 X 10MM C92729 M4 533 77 MASS(ES) INGUINAL REGION-LEFT WEEK 77 15 X 15MM 540 78 TO 549 79 MASS(ES) INGUINAL REGION-LEFT WEEK 77 20 X 20MM C92735 M4 183 27 TO 190 28 MASS(ES) VENTRAL-ABDOMINAL WEEK 27 10 X 10MM C92737 M4 554 80 TO 637 91 MASS(ES) DORSAL WEEK 78 10 X 10MM 719 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 178 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92740 M4 645 93 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 93 15 X 15MM C92742 M4 351 51 TO 379 55 MASS(ES) DORSAL WEEK 51 10 X 10MM 386 56 TO 407 59 MASS(ES) DORSAL WEEK 51 15 X 15MM C92745 M4 512 74 TO 533 77 MASS(ES) DORSAL WEEK 74 15 X 15MM 540 78 TO 547 79 MASS(ES) DORSAL WEEK 74 20 X 20MM 554 80 TO 582 84 MASS(ES) DORSAL WEEK 74 25 X 20MM 596 86 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 10 X 10MM 316 46 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 10 X 10MM 720 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 179 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92745 M4 330 48 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 15 X 15MM 337 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 10 X 10MM 484 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 10 X 10MM 491 71 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 20 X 20MM 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 25 X 25MM 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 30 X 30MM 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 20 X 15MM 721 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 180 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92751 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C92752 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92753 T 105 APPEARANCE MALOCCLUSION MISSING TEETH THIN 43, 50, 57, 64, 71, 148, 155, 162, 169, 232, 239, 246, 253, 323, 330, 337, 344, 414, 421, 428, 435, 505, 512, 519, 526, 596, 603, 610, 617, 687, 694, 701, 708, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 173, 176, 183, 190, 197, 204, 211, 218, 225 260, 267, 274, 281, 288, 295, 302, 309, 316 351, 358, 365, 372, 379, 386, 393, 400, 407 442, 449, 456, 463, 470, 477, 484, 491, 498 533, 540, 547, 554, 561, 568, 575, 582, 589 624, 631, 638, 645, 652, 659, 666, 673, 680 715, 722, 729, 733 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 733 173, 176, 183 DISCHARGE GENITAL RED IN COLOR 666, 680, 687 EXCRETION FEW FECES 173 722 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 181 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92753 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 631, 638, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 MASS(ES) INGUINAL REGION-RIGHT WEEK 90 624C C92754 3 53 APPEARANCE MALOCCLUSION 57, 64, 71, 78, 85, 92, 99, 106 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 113, 120, 127, 134, 141, 148, 155 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351, 358, 365-366 C92755 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 723 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 182 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92756 M 88 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 614 EXCRETION FEW FECES 590, 596, 610, 614 SKIN & PELAGE SORE/SCAB DORSAL 582, 589-590, 596, 603, 610, 614 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) DORSAL WEEK 84 582, 589-590, 596, 603, 610, 614 C92757 T 105 APPEARANCE MALOCCLUSION 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 731 724 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 183 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92757 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 449, 456 MASS(ES) DORSAL WEEK 98 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C C92758 M 62 APPEARANCE THIN 400, 407, 414, 421, 428 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 428 EXCRETION FEW FECES 393, 414, 421, 428 SKIN & PELAGE ROUGH HAIR COAT 414, 421, 428 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 725 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 184 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92759 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92760 M 74 APPEARANCE THIN 512 EXCRETION FEW FECES 503, 505, 512 EYES RED DISCHARGE EYES 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92761 T 105 APPEARANCE MALOCCLUSION 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708 EXCRETION FEW FECES 596 EYES PROTRUDING EYES 603, 610 726 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 185 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92761 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 715, 722, 729, 732 C92762 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 491, 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 519, 526, 610, 617, 701, 708, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 533, 540, 547, 554, 624, 631, 638, 645, 715, 722, 729, 731 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 561, 568, 575, 582, 652, 659, 666, 673, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 512 589, 596, 603 680, 687, 694 MASS(ES) VENTRAL-ABDOMINAL WEEK 68 470, 477, 484C, 491C, 498C, 505 727 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 186 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92763 M 94 APPEARANCE OBES SWOLLEN PAW-HIND-RIGHT PAWS-HIND 596, 603, 610 631 638, 645, 652-653 EXCRETION FEW FECES 652-653 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT 653 638, 645, 652-653 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 617, 624 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92764 M 76 APPEARANCE HUNCHED THIN 530 505, 512, 519, 526, 530 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 526, 530 530 728 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 187 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92764 M 76 BEHAVIOR COLD TO TOUCH LIMBS-ALL 530 TAIL 530 EXCRETION FEW FECES 512, 519, 526, 530 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92765 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92766 D 101 EYES RED DISCHARGE EYE-RIGHT 666 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 358 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 365, 372, 379, 386, 393, 400 729 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 188 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92766 D 101 NORMAL NO REMARKABLE OBSERVATIONS 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 673, 680 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 RECTUM WEEK 99 344, 351 687, 694, 701, 705 C92767 D 99 APPEARANCE OBES 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 638, 645, 652, 659 666, 673, 680, 687, 691 EXCRETION FEW FECES 673 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 691 659, 666, 673, 680, 687, 691 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456 730 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 189 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92768 M 95 SKIN & PELAGE BLACK SKIN DORSAL SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-RIGHT 660 540, 547 631, 638, 645, 652, 659-660 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 554, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 MASS(ES) DORSAL WEEK 85 INGUINAL REGION-RIGHT WEEK 90 VENTRAL-ABDOMINAL WEEK 77 589, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659-660C 624 533C C92769 D 103 APPEARANCE LIMITED USE PAWS-ALL 715, 721 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL 624 731 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 190 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92769 D 103 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 631, 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 708 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 645, 652, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 659, 666, 673, 680, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 687, 694, 701 MASS(ES) INGUINAL REGION-LEFT WEEK 86 INGUINAL REGION-RIGHT WEEK 86 VENTRAL-ABDOMINAL WEEK 89 596C, 603C, 610C 596C, 603C, 610C 617C, 624C C92770 D 101 APPEARANCE LIMITED USE LIMBS-ALL PAW-HIND-LEFT SWOLLEN LIMBS-HIND PAW-HIND-LEFT 694, 701-702 539-540, 547, 554, 561, 568, 575, 582 694, 701-702 539-540, 547, 554, 561, 568, 575, 582 732 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 191 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92770 D 101 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 701-702 SKIN & PELAGE YELLOW HAIR COAT 701-702 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 617, 624, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 631, 638, 645, 652, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 589, 659, 666 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 596, 603, 610 MASS(ES) PAW-FRONT-LEFT WEEK 97 673, 680, 687, 694, 701-702 C92771 M 81 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 567 EXCRETION NO FECES 567 EYES RED DISCHARGE EYES 561, 567 733 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 192 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92771 M 81 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 561, 567 VENTRAL-ABDOMINAL 561, 567 ROUGH HAIR COAT 561, 567 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 C92772 M 52 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 360 SURGERY PERFORMED BY KATHY EWERS 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359 734 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 193 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92773 D 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT VENTRAL-ABDOMINAL-LEFT THIN 428, 435, 442, 449, 456, 463 715, 722, 729, 731 589, 596, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 519, 526, 638, 645, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 533, 540, 547, 554, 652, 659, 666, 673, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 470, 477, 484, 491, 561, 568, 575, 582, 680, 687, 694, 701, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 498, 505, 512 617, 624, 631 708 C92774 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 624, 708, 715, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 631, 638, 645, 652, 722, 729, 733 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 659, 666, 673, 680, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 687, 694, 701 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 575, 582, 589, 596, 603, 610 735 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 194 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92775 T 105 APPEARANCE MISSING TEETH 344, 351 EXCRETION FEW FECES 729, 732 SKIN & PELAGE SORE/SCAB DORSAL 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 400, 407, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 414, 421, 428, 435, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 358, 365, 372, 442, 449, 456, 463, 92, 99, 106 183, 190, 197 274, 281, 288 379, 386, 393 470, 477, 484 MASS(ES) DORSAL WEEK 72 498, 505, 512, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 732C 736 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 195 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92776 T 105 DISCHARGE GENITAL RED IN COLOR 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701 57, 64, 71, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 WART LIKE LESION(S) MOUTH WEEK 102 708, 715, 722, 729, 732 C92777 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92778 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY JOHN VAN BERKUM 359 737 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 196 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92778 3 53 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92779 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92780 T 105 APPEARANCE HUNCHED 729, 733 EXCRETION FEW FECES 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92781 M 77 APPEARANCE THIN TREMORS ENTIRE BODY 498, 503, 505, 507, 512, 519, 526, 533, 535 535 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 535 738 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 197 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92781 M 77 BEHAVIOR COLD TO TOUCH ENTIRE BODY 533, 535 EXCRETION FEW FECES 503, 505, 507, 512, 519, 526, 533, 535 SKIN & PELAGE PALE ENTIRE BODY 519, 526, 533, 535 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92782 T 105 APPEARANCE OBES 596, 603, 610 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 739 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 198 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92782 T 105 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 103 715C, 722C, 729, 732 C92783 M 79 APPEARANCE THIN 547 BEHAVIOR RECUMBENT STERNAL 547 COLD TO TOUCH ENTIRE BODY 547 EXCRETION NO FECES 547 EYES RED DISCHARGE EYE-RIGHT 512, 519, 526, 533, 540, 547 RESPIRATION AUDIBLE 547 LABORED 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 740 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 199 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92783 M 79 NORMAL NO REMARKABLE OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 C92784 M 80 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 556 EXCRETION FEW FECES NONFORMED FECES 555-556 555-556 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 C92785 D 98 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 686 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 741 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 200 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92786 T 105 EXCRETION NONFORMED FECES 729 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 323, 407, 414, 498, 505, 589, 596, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 358, 365, 372, 379, 449, 456, 463, 470, 540, 547, 554, 561, 631, 638, 645, 652, 722, 731 92, 99, 106 183, 190, 197 274, 281, 288 386, 393, 400 477, 484, 491 568, 575, 582 659, 666, 673 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 302C, 309C, 316C C92787 D 47 APPEARANCE LIMITED USE LIMB-HIND-LEFT LIMBS-HIND SWOLLEN LIMB-HIND-LEFT 302, 309, 316 323, 328 309 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295 742 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 201 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92788 T 105 APPEARANCE MALOCCLUSION 106, 113 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 127, 134, 218, 225, 309, 316, 400, 407, 491, 498, 582, 589, 673, 680, 22, 29, 36, 43, 50, 141, 148, 155, 162, 232, 239, 246, 253, 323, 330, 337, 344, 414, 421, 428, 435, 505, 512, 519, 526, 596, 603, 610, 617, 687, 694, 701, 708, 57, 64, 71, 78, 85, 169, 176, 183, 190, 260, 267, 274, 281, 351, 358, 365, 372, 442, 449, 456, 463, 533, 540, 547, 554, 624, 631, 638, 645, 715, 722, 729, 733 92, 99, 120 197, 204, 211 288, 295, 302 379, 386, 393 470, 477, 484 561, 568, 575 652, 659, 666 C92789 2 14 APPEARANCE MALOCCLUSION 92-93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85 C92790 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 743 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 202 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92791 T 105 SKIN & PELAGE SORE/SCAB DORSAL 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 MASS(ES) DORSAL WEEK 93 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 731C C92792 T 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 744 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 203 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92792 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 101 701C, 708C, 715C, 722C, 729C, 732C VENTRAL-ABDOMINAL WEEK 104 722, 729, 732 C92793 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92794 D 58 APPEARANCE THIN 400, 402 EXCRETION FEW FECES 393, 400, 402 EYES RED DISCHARGE EYE-RIGHT 204, 211, 225, 232, 365, 372, 379, 386, 393, 400, 402 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 400, 402 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 218, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358 745 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 204 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92795 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY KATHY EWERS 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92796 3 53 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB LIMB-FRONT-RIGHT 239, 323, 330, 337, 344, 351, 358 92 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 365-366 SURGERY PERFORMED BY JOHN VAN BERKUM 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 746 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 205 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92797 M 94 APPEARANCE LIMITED USE LIMB-HIND-RIGHT MALOCCLUSION MISSING TEETH 653 596, 603, 610 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652-653C C92798 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92799 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 747 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 206 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92800 T 105 DISCHARGE GENITAL RED IN COLOR 50 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 57, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701, 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 708, 715, 722, 729, 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 731 C92801 D 57 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 VENTRAL-ABDOMINAL-RIGHT WEEK 53 386C, 393C 365C, 372C, 379C, 386C, 393C 748 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 207 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92802 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92803 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 733 C92804 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 400, 407, 491, 498, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 414, 421, 428, 435, 505, 512, 519, 526, 603, 610, 617, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 442, 449, 456, 463, 533, 540, 547, 554, 631, 638, 645, 652, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 470, 477, 484 568, 575, 582 659, 666, 673 749 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 208 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92804 T 105 NORMAL NO REMARKABLE OBSERVATIONS 680, 687, 694, 701, 708, 715, 722, 729, 731 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 386C, 393C C92805 M 72 APPEARANCE THIN 498, 500 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 498, 500 EYES RED DISCHARGE EYE-LEFT 500 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT VENTRAL-ABDOMINAL-LEFT 484, 491, 498 463, 470, 477, 484, 491, 498, 500 456, 463 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 750 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 209 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92805 M 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 65 449 C92806 M 99 APPEARANCE MALOCCLUSION MISSING TEETH THIN 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106, 113, 120, 127, 134, 141, 148 183, 190, 197, 204, 211, 218, 225, 232, 239 274, 281, 288, 295, 302, 309, 316, 323, 330 365, 372, 379, 386, 393, 400, 407, 414, 421 456, 463, 470, 477, 484, 491, 498, 505, 512 547, 554, 561, 568, 575, 582, 589, 596, 603 638, 645, 652, 659, 666, 673, 680, 687, 691 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 691 687, 691 EXCRETION FEW FECES NO FECES 680, 687 691 RESPIRATION IRREGULAR LABORED 687, 691 680, 687, 691 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 596, 680, 687, 691 751 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 210 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92806 M 99 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 C92807 D 86 EXCRETION FEW FECES 596-597 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 295 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 330, 337, 421, 428, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 302, 372, 379, 386, 393, 463, 470, 477, 484, 554, 561, 568, 575, 92, 99, 106 183, 190, 197 309, 316, 323 400, 407, 414 491, 498, 505 582, 589 MASS(ES) VENTRAL-ABDOMINAL WEEK 39 267C, 274C, 281C, 288C C92808 T 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 752 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 211 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92808 T 105 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-LEFT WEEK 76 WEEK 103 INGUINAL REGION-LEFT WEEK 98 VENTRAL-ABDOMINAL-LEFT WEEK 98 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 715, 722, 729C, 731C 680, 687, 694, 701, 708, 715, 722, 729C, 731C 680, 687, 694, 701, 708, 715, 722, 729, 731 C92809 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 484, 491, 498, 505, 610, 617 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 512, 519, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 526, 533, 540, 547, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 554, 561, 568, 575, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 582 MASS(ES) AXILLARY REGION-LEFT WEEK 85 INGUINAL REGION-LEFT WEEK 86 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 596C, 603C 753 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 212 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92809 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 477C, 484C, 491C, 498C C92810 M 97 APPEARANCE THIN 659, 666, 673 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 673 EXCRETION FEW FECES 656, 659, 666, 673 EYES SQUINTED PERI-ORBITAL 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 754 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 213 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92811 M 36 APPEARANCE LIMITED USE LIMBS-HIND 250 EXCRETION FEW FECES 250 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246 C92812 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92813 M 100 APPEARANCE THIN 694 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 694 EXCRETION FEW FECES 694 EYES SQUINTED PERI-ORBITAL 694 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 576 755 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 214 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92813 M 100 SKIN & PELAGE RED HAIR COAT SHOULDER-RIGHT 576 SORE/SCAB DORSAL 638, 645, 652, 659, 666, 673, 680, 687, 694 DORSAL-THORACIC-LEFT 631 HEAD-ENTIRE 561, 568, 575-576 PAW-HIND-RIGHT 638, 645, 652, 659, 666, 673, 680, 687, 694 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 582 MASS(ES) DORSAL WEEK 87 WEEK 99 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C 687C C92814 T 105 APPEARANCE MALOCCLUSION 519, 526, 533, 540, 547, 554, 610, 638, 645, 652, 659, 715, 722 729, 733 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 344 756 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 215 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92814 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 561, 568, 575, 582, 589, 596, 603, 617, 624, 631 666, 673, 680, 687, 694, 701, 708 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 330, 337C C92815 T 105 APPEARANCE MALOCCLUSION MISSING TEETH 43, 50, 57, 64, 71, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 694, 701, 708, 715, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484, 491, 498, 505 540, 547, 554, 561, 568, 575, 582, 589, 596 631, 638, 645, 652, 659, 666, 673, 680, 687 722, 729, 732 610, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 732 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT 64, 71, 78, 85, 92, 99, 106, 113 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 757 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 216 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92816 D 64 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 446 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C92817 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY PATTIE MURPHY 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92818 T 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT 680, 687, 694, 701, 708, 715, 722, 729, 732 DISCHARGE GENITAL RED IN COLOR 176 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 183, 190, 197, 204 758 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 217 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92818 T 105 NORMAL NO REMARKABLE OBSERVATIONS 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 372, 379, 386, 393 C92819 D 104 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-LEFT DORSAL-THORACIC-LEFT PAW-HIND-LEFT VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 680, 687, 694, 701, 708, 715, 722, 727 239, 246, 253, 260 666, 673, 680, 687, 694, 701, 708, 715, 722, 727 281, 288, 295 141 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 148, 155, 162 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 169, 176, 183, 190C, 197C, 204C, 211C, 218C, 225C, 232C, 239C 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 759 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 218 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92819 D 104 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 727C VENTRAL-ABDOMINAL-LEFT WEEK 40 274C, 281C, 288C C92820 3 53 APPEARANCE MALOCCLUSION 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365-366 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64 760 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 219 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92753 M5 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 90 10 X 10MM C92757 M5 680 98 TO 731 105 MASS(ES) DORSAL WEEK 98 10 X 10MM C92762 M5 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL WEEK 68 10 X 10MM C92768 M5 596 86 MASS(ES) DORSAL WEEK 85 10 X 10MM 603 87 MASS(ES) DORSAL WEEK 85 15 X 15MM 610 88 MASS(ES) DORSAL WEEK 85 30 X 30MM 617 89 MASS(ES) DORSAL WEEK 85 40 X 40MM 624 90 MASS(ES) DORSAL WEEK 85 60 X 60MM 761 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 220 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92768 M5 631 91 MASS(ES) DORSAL WEEK 85 80 X 70MM 638 92 TO 652 94 MASS(ES) DORSAL WEEK 85 70 X 60MM 659 95 TO 660 95 MASS(ES) DORSAL WEEK 85 80 X 100MM 533 77 MASS(ES) VENTRAL-ABDOMINAL WEEK 77 10 X 10MM C92769 M5 596 86 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 86 10 X 10MM 596 86 TO 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 86 10 X 10MM 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 20 X 20MM C92775 M5 519 75 TO 554 80 MASS(ES) DORSAL WEEK 72 10 X 10MM 762 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 221 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92775 M5 561 81 TO 582 84 MASS(ES) DORSAL WEEK 72 20 X 20MM 589 85 TO 673 97 MASS(ES) DORSAL WEEK 72 15 X 15MM 680 98 TO 715 103 MASS(ES) DORSAL WEEK 72 20 X 20MM 722 104 TO 732 105 MASS(ES) DORSAL WEEK 72 10 X 10MM C92782 M5 715 103 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 103 10 X 10MM C92786 M5 302 44 TO 316 46 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 10 X 15MM C92791 M5 645 93 TO 673 97 MASS(ES) DORSAL WEEK 93 10 X 10MM 680 98 TO 715 103 MASS(ES) DORSAL WEEK 93 15 X 15MM 763 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 222 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92791 M5 722 104 TO 731 105 MASS(ES) DORSAL WEEK 93 20 X 20MM C92792 M5 701 101 TO 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 101 20 X 20MM 715 103 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 101 30 X 30MM 729 105 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 101 50 X 60 MM C92797 M5 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 40 X 40MM 589 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 40 X 50MM 596 86 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 60 X 60MM 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 70 X 60MM 764 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 223 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92797 M5 617 89 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 80 X 70MM 624 90 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 100 X 80MM 631 91 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 110 X 100MM 645 93 TO 653 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 120 X 110MM C92801 M5 386 56 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 70 X 80MM 365 53 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 15 X 15MM C92804 M5 386 56 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 10 X 10MM C92807 M5 267 39 TO 274 40 MASS(ES) VENTRAL-ABDOMINAL WEEK 39 10 X 10MM 765 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 224 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92807 M5 281 41 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL WEEK 39 15 X 15MM C92808 M5 729 105 TO 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 103 10 X 10 MM 729 105 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 98 10 X 10 MM C92809 M5 589 85 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 85 25 X 20MM 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 85 30 X 30MM 638 92 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 85 30 X 40MM 666 96 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 85 35 X 40MM 680 98 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 85 50 X 45MM 766 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 225 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92809 M5 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 85 50 X 50MM 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 85 60 X 70MM 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 85 70 X 60MM 729 105 TO 733 105 MASS(ES) AXILLARY REGION-LEFT WEEK 85 70 X 80 MM 596 86 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 86 15 X 15MM 477 69 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 10 X 10MM C92813 M5 603 87 TO 694 100 MASS(ES) DORSAL WEEK 87 10 X 10MM 687 99 MASS(ES) DORSAL WEEK 99 15 X 15MM 767 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 226 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92814 M5 337 49 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 10 X 10MM C92819 M5 190 28 TO 204 30 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 10 X 10MM 211 31 TO 295 43 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 20 X 20MM 302 44 TO 323 47 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 25 X 25MM 330 48 TO 365 53 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 25 X 20MM 372 54 TO 407 59 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 30 X 20MM 414 60 TO 442 64 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 30 X 30MM 449 65 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 40 X 30MM 768 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 227 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92819 M5 456 66 TO 470 68 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 30 X 30MM 477 69 TO 505 73 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 40 X 30MM 512 74 TO 533 77 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 50 X 40MM 540 78 TO 582 84 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 50 X 50MM 589 85 TO 610 88 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 70 X 60MM 617 89 TO 624 90 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 60 X 50MM 631 91 TO 638 92 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 75 X 55MM 645 93 TO 666 96 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 80 X 55MM 769 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 228 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92819 M5 673 97 TO 708 102 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 80 X 60MM 715 103 TO 727 104 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 60 X 50MM 274 40 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 40 10 X 10MM 770 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 229 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92821 U 106 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-LEFT 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 736 344, 351, 358, 365, 372, 379, 386, 393, 400, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 736 729, 736 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155 771 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 230 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92822 M 81 APPEARANCE HEAD TILT LEFT THIN 566 554, 561, 566 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 561, 566 554, 561, 566 EXCRETION FEW FECES 566 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 456, 463, 470 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 498, 505, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 512, 519, 526, 533, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 540, 547 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 SACRIFICE SACRIFICE APPROVED BY CERTIFIED TECHNICIAN 566 772 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 231 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92823 U 106 APPEARANCE MALOCCLUSION MISSING TEETH 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 736 736 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 435, 442, 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29 MASS(ES) VENTRAL-ABDOMINAL WEEK 74 512C, 519C, 526C C92824 D 95 APPEARANCE THIN 535 EXCRETION FEW FECES 535 LIQUID FECES 535 NO FECES 582 773 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 232 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92824 D 95 RESPIRATION AUDIBLE 582, 589, 596 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND 535 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407 456, 463 470, 477, 484, 491, 498, 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 414, 421, 428, 435, 442, 449 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 535C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 663C C92825 D 94 APPEARANCE SWOLLEN LIMB-HIND-LEFT PAW-HIND-LEFT 428, 435, 442, 449, 456, 463 428, 435, 442, 449, 456, 463 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 540, 547, 554 774 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 233 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92825 D 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 470, 477, 484, 491, 498, 505, 512 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652-653 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 519C, 526C, 533C, 540C, 547C C92826 D 55 DISCHARGE CLEAR ORAL <UNSPECIFIED> 385 RESPIRATION AUDIBLE 385 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 45 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 385 775 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 234 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92827 U 106 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT DORSAL 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) DORSAL WEEK 92 VENTRAL-ABDOMINAL-RIGHT WEEK 97 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729C, 736C 673C, 680C, 687C, 694C, 701C, 708C WART LIKE LESION(S) AXILLARY REGION-RIGHT WEEK 87 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715 776 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 235 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92828 D 83 APPEARANCE MALOCCLUSION SWOLLEN TESTIS-LEFT 470, 512 533, 540, 547, 554, 561, 568, 575, 578 DISCHARGE NASAL RED IN COLOR 197 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 211, 218, 302, 309, 393, 400, 491, 498, 22, 29, 36, 43, 50, 127, 134, 141, 148, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 505, 519, 526 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 204 281, 288, 295 372, 379, 386 463, 477, 484 C92829 U 106 APPEARANCE SWOLLEN PAW-HIND-LEFT PAW-HIND-RIGHT 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 736 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 777 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 236 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92829 U 106 NORMAL NO REMARKABLE OBSERVATIONS 407, 414, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 281C, 288C, 295C WART LIKE LESION(S) TAIL WEEK 61 421, 428, 435 C92830 M 99 APPEARANCE MALOCCLUSION THIN 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 690 666, 673, 680, 687, 690 BEHAVIOR COLD TO TOUCH ENTIRE BODY 690 EXCRETION FEW FECES 687, 690 RESPIRATION IRREGULAR 687, 690 778 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 237 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92830 M 99 SKIN & PELAGE SORE/SCAB EAR-RIGHT 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 690 OTHER 690C NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 MASS(ES) EAR-RIGHT WEEK 47 323, 330, 337, 344, 351, 358, 407C, 414C, 421C, 428C, 435C, 484C, 491C, 498C, 505C, 512C, 561C, 568C, 575C, 582C, 589C, 638C, 645C, 652C, 659C, 666C, 365, 372C, 379C, 386C, 393C, 400C 442C, 449C, 456C, 463C, 470C, 477C 519C, 526C, 533C, 540C, 547C, 554C 596C, 603C, 610C, 617C, 624C, 631C 673C, 680C, 687C, 690C WART LIKE LESION(S) EAR-RIGHT WEEK 41 281, 288 C92831 M 85 APPEARANCE LIMITED USE LIMBS-ALL LIMBS-HIND MALOCCLUSION 585, 589 582 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 779 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 238 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92831 M 85 APPEARANCE MALOCCLUSION 575, 582, 585, 589 SWOLLEN NOSE 92 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 589 RECUMBENT STERNAL 589 COLD TO TOUCH ENTIRE BODY 589 EYES RED DISCHARGE EYE-RIGHT 92, 589 SKIN & PELAGE SORE/SCAB MOUTH 92 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 780 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 239 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92832 D 69 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 480 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92833 U 106 APPEARANCE OBES SWOLLEN LIMB-HIND-RIGHT PAW-HIND-RIGHT 477 622, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 736 680, 687, 694, 701, 708, 715, 722, 729, 736 SKIN & PELAGE RED SKIN PAW-HIND-RIGHT 622 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 554, 561, 568 781 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 240 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92834 U 106 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB SHOULDER-RIGHT 379 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 MASS(ES) SHOULDER-RIGHT WEEK 55 379, 386, 393, 400, 407C, 414C, 421C, 428C, 435C, 442C, 449C 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C WART LIKE LESION(S) SHOULDER-RIGHT WEEK 95 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 736 C92835 M 75 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 522 RECUMBENT DORSAL 522 COLD TO TOUCH ENTIRE BODY 522 782 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 241 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92835 M 75 EXCRETION FEW FECES 522 EYES RED DISCHARGE EYE-RIGHT EYES 246, 281 522 RESPIRATION LABORED 522 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 309, 316, 400, 407, 491, 498, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 323, 330, 337, 344, 414, 421, 428, 435, 505, 512, 519 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 351, 358, 365, 372, 442, 449, 456, 463, 92, 99, 106 183, 190, 197 288, 295, 302 379, 386, 393 470, 477, 484 C92836 D 91 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 631 RESPIRATION LABORED 631 SKIN & PELAGE PALE ENTIRE BODY 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 783 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 242 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92836 D 91 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624 C92837 M 65 APPEARANCE THIN 449 EXCRETION FEW FECES 445, 449 SKIN & PELAGE ROUGH HAIR COAT 445, 449 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C92838 M 65 EXCRETION FEW FECES NONFORMED FECES 449-450 450 SKIN & PELAGE BROWN HAIR COAT SCROTUM 450 784 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 243 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92838 M 65 SKIN & PELAGE SORE/SCAB DORSAL 365, 372, 379, 386 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 414, 421, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 428, 435 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 393, 400, 407 MASS(ES) DORSAL WEEK 53 365, 372, 379 WART LIKE LESION(S) DORSAL WEEK 64 442, 449-450 C92839 D 89 APPEARANCE OBES 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610 EYES RED DISCHARGE EYE-LEFT 575, 617, 623 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 785 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 244 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92839 D 89 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 C92840 M 103 APPEARANCE THIN 715 DISCHARGE NASAL RED IN COLOR 708 EXCRETION FEW FECES 701, 708, 715 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 786 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 245 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92841 D 68 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 476 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92842 M 91 APPEARANCE CONVULSIONS 633 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 633 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 633 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 88 610, 617, 624 787 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 246 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92842 M 91 WART LIKE LESION(S) DORSAL WEEK 77 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 633 C92843 D 77 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 537 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92844 M 83 APPEARANCE MALOCCLUSION THIN 295, 428, 435, 442, 463, 470, 477 579 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 579 EXCRETION FEW FECES 579 EYES RED DISCHARGE EYE-RIGHT 579 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 788 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 247 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92844 M 83 NORMAL NO REMARKABLE OBSERVATIONS 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 449, 456, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575 C92845 M 95 APPEARANCE HEAD TILT RIGHT 663-664 BEHAVIOR RECUMBENT LATERAL 664 EXCRETION FEW FECES 663 NO FECES 664 RESPIRATION IRREGULAR 664 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 789 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 248 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92846 D 103 APPEARANCE MALOCCLUSION 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 721 EXCRETION FEW FECES 603 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547 393 659, 666, 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 MASS(ES) DORSAL WEEK 55 DORSAL-CERVICAL-RIGHT WEEK 69 INGUINAL REGION-LEFT WEEK 91 379, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 631C, 638C, 645C, 652C 790 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 249 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92846 D 103 WART LIKE LESION(S) DORSAL WEEK 80 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 721 C92847 M 98 APPEARANCE THIN 680 EXCRETION FEW FECES 673, 680 EYES RED DISCHARGE EYE-LEFT 680 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 791 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 250 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92848 U 106 APPEARANCE LIMITED USE PAW-HIND-LEFT SWOLLEN LIMB-HIND-LEFT PAW-HIND-LEFT THIN 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 736 585, 589, 596, 603, 610 585, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 736 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C92849 M 70 APPEARANCE MALOCCLUSION 484 BEHAVIOR HYPOACTIVE MODERATE COLD TO TOUCH LIMBS-ALL ENTIRE BODY 484 470, 477, 484 484 EXCRETION FEW FECES NO FECES 470, 477, 484 484 792 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 251 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92849 M 70 EYES SQUINTED PERI-ORBITAL 477, 484 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463 C92850 M 105 APPEARANCE MALOCCLUSION SWOLLEN LIMB-HIND-RIGHT PAW-HIND-RIGHT THIN 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 659, 666, 673 659, 666, 673 722, 729-730 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 730 RECUMBENT LATERAL 730 EXCRETION NONFORMED FECES 344 SKIN & PELAGE PALE ENTIRE BODY 722, 729-730 793 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 252 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92850 M 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 351, 358, 365, 372, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 680, 687, 694, 701, 708, 715 C92851 U 106 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 554, 561, 568 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 477 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 102 708, 715, 722, 729, 736 794 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 253 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92852 M 68 APPEARANCE HUNCHED THIN 475 474-475 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 475 EXCRETION FEW FECES 474-475 RESPIRATION IRREGULAR 475 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92853 U 106 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 736 795 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 254 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92854 D 101 EXCRETION NONFORMED FECES 113 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 617, 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 575, 596, 22, 29, 36, 43, 50, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 638, 645, 652, 659, 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 666, 673, 680, 694, 92, 99, 106 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 701, 705 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 WEEK 87 582, 589 603, 610 WART LIKE LESION(S) VENTRAL-ABDOMINAL-RIGHT WEEK 99 687 796 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 255 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92855 M 70 APPEARANCE HUNCHED MALOCCLUSION 484 50, 57, 64, 71, 78, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 484 DISCHARGE GENITAL RED IN COLOR 176, 183 SKIN & PELAGE ROUGH HAIR COAT 463, 470, 477, 484 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 WART LIKE LESION(S) VENTRAL-ABDOMINAL-LEFT WEEK 69 477, 484 797 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 256 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92856 U 106 APPEARANCE BENT TAIL SWOLLEN PAW-HIND-RIGHT 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 595-596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 736 595-596, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 MASS(ES) INGUINAL REGION-LEFT WEEK 99 687, 694, 701, 708, 715, 722, 729, 736 C92857 M 83 APPEARANCE SWOLLEN PAW-HIND-LEFT 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 576 RECUMBENT STERNAL 576 EXCRETION FEW FECES 575 NO FECES 576 798 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 257 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92857 M 83 EYES RED DISCHARGE EYES 576 SQUINTED PERI-ORBITAL 576 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 554, 561, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 477, 484, 491, 498, 568 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 505, 512, 519, 526, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 533, 540, 547 C92858 M 97 APPEARANCE THIN 673 EXCRETION FEW FECES 659, 666, 673 RESPIRATION AUDIBLE 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 540, 547, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 554, 561, 568, 575, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 582, 589, 596 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 799 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 258 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92858 M 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 631C, 638C, 645C, 652C, 659C, 666C, 673C INGUINAL REGION-LEFT WEEK 69 477, 484, 491, 498, 505, 512, 519, 526, 533 WART LIKE LESION(S) DORSAL WEEK 76 WEEK 87 526, 533 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 C92859 M 72 APPEARANCE HEAD TILT RIGHT 498, 500 EXCRETION NONFORMED FECES 393 SKIN & PELAGE ROUGH HAIR COAT 491, 498, 500 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 400, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 800 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 259 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: M6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92860 M 97 APPEARANCE LIMITED USE LIMBS-HIND 673, 678 BEHAVIOR ATAXIC <UNSPECIFIED> 678 EXCRETION FEW FECES 673, 678 EYES RED DISCHARGE EYES 678 SKIN & PELAGE ROUGH HAIR COAT 678 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 WART LIKE LESION(S) VENTRAL-THORACIC-LEFT WEEK 96 666, 673, 678 801 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 260 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92823 M6 512 74 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL WEEK 74 15 X 15MM C92824 M6 456 66 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 15 X 15MM 463 67 TO 470 68 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 25 X 15MM 477 69 TO 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 30 X 25MM 498 72 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 35 X 30MM 526 76 TO 535 77 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 40 X 35MM 540 78 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 50 X 50MM 554 80 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 45 X 40MM 802 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 261 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92824 M6 589 85 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 45 X 60MM 631 91 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 50 X 60MM 659 95 TO 663 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 50 X 70MM C92825 M6 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 10 X 10MM 526 76 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 15 X 15MM C92827 M6 638 92 TO 673 97 MASS(ES) DORSAL WEEK 92 10 X 10MM 680 98 TO 715 103 MASS(ES) DORSAL WEEK 92 15 X 10MM 722 104 TO 736 106 MASS(ES) DORSAL WEEK 92 10 X 10MM 803 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 262 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92827 M6 673 97 TO 708 102 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 10 X 10MM C92829 M6 281 41 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 20 X 20MM 288 42 TO 295 43 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 10 X 10MM C92830 M6 690 99 SKIN & PELAGE OTHER COLD TO TOUCH 372 54 TO 379 55 MASS(ES) EAR-RIGHT WEEK 47 10 X 10MM 386 56 TO 610 88 MASS(ES) EAR-RIGHT WEEK 47 10 X 15MM 617 89 TO 659 95 MASS(ES) EAR-RIGHT WEEK 47 20 X 20MM 666 96 TO 690 99 MASS(ES) EAR-RIGHT WEEK 47 30 X 20MM 804 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 263 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92834 M6 407 59 TO 491 71 MASS(ES) SHOULDER-RIGHT WEEK 55 10 X 10MM 498 72 TO 568 82 MASS(ES) SHOULDER-RIGHT WEEK 55 15 X 10MM 575 83 TO 582 84 MASS(ES) SHOULDER-RIGHT WEEK 55 20 X 10MM C92846 M6 386 56 TO 449 65 MASS(ES) DORSAL WEEK 55 15 X 10MM 477 69 TO 505 73 MASS(ES) DORSAL-CERVICAL-RIGHT WEEK 69 10 X 10MM 512 74 TO 533 77 MASS(ES) DORSAL-CERVICAL-RIGHT WEEK 69 15 X 15MM 631 91 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 91 15 X 15MM 645 93 TO 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 91 20 X 20MM 805 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 264 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92858 M6 631 91 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 10 X 10MM 652 94 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 15 X 15MM 673 97 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 15 X 20MM 806 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume III of IX Page 807 of 4068 Covance 6329-183 3M T-6295 VOLUME III OF IX CONTENTS Page APPENDIX 2 (Continued) .......................................................................................... 809 Individual Clinical Observations.............................................................................. 810 808 APPENDIX 2 (Continued) Individual Clinical Observations Covance 6329-183 3M T-6295 809 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 265 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92861 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92862 3 53 APPEARANCE MALOCCLUSION 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 316, 330, 337, 344, 351 358, 365-366 EXCRETION NONFORMED FECES 274 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 281, 288, 295, 302, 309, 323 C92863 M 83 APPEARANCE MISSING TAIL-DISTAL 575 SKIN & PELAGE BLACK SKIN TAIL 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 810 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 266 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92864 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92865 D 65 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 351, 358, 442, 449 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 365, 372, 379, 386, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 393, 400, 407, 414, 92, 99, 106 183, 190, 197 274, 337, 344 421, 428, 435 MASS(ES) AXILLARY REGION-LEFT WEEK 46 VENTRAL-ABDOMINAL WEEK 41 VENTRAL-CERVICAL-LEFT WEEK 47 316, 323, 330 281C, 288C, 295C, 302C, 309C, 316C, 323C 323, 330 C92866 T 105 APPEARANCE LIMITED USE LIMB-HIND-LEFT SWOLLEN PAW-HIND-LEFT 239, 246 239, 246 EXCRETION FEW FECES NO FECES 715, 717, 722, 729, 733 691 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 715, 717, 722 811 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 267 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92866 T 105 SKIN & PELAGE SORE/SCAB MOUTH 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708 C92867 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) VENTRAL-ABDOMINAL WEEK 104 722C, 729C, 732C 812 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 268 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92868 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92869 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C92870 M 105 APPEARANCE HEAD TILT LEFT MALOCCLUSION SWOLLEN LIMB-HIND-LEFT PAW-HIND-RIGHT THIN 729 246, 253, 260, 267, 645, 652, 722, 729 645 673 729 BEHAVIOR ATAXIC <UNSPECIFIED> 729 813 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 269 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92870 M 105 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 729 RECUMBENT LATERAL 729 EXCRETION FEW FECES 717, 722, 729 EYES RED DISCHARGE EYE-RIGHT 717 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 323, 330, 414, 421, 505, 512, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 57, 64, 71, 78, 85, 155, 162, 169, 176, 274, 281, 288, 295, 365, 372, 379, 386, 456, 463, 470, 477, 547, 554, 561, 568, 638, 659, 666, 680 92, 99, 106 183, 190, 197 302, 309, 316 393, 400, 407 484, 491, 498 575, 582, 589 MASS(ES) AXILLARY REGION-LEFT WEEK 99 INGUINAL REGION-LEFT WEEK 105 687, 694, 701, 708, 715, 717, 722, 729 729 814 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 270 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92871 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 94 VENTRAL-ABDOMINAL WEEK 84 VENTRAL-THORACIC-LEFT WEEK 76 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 731C 582 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C C92872 M 89 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 617-618 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 MASS(ES) INGUINAL REGION-LEFT WEEK 83 575, 582, 589, 596C, 603C, 610C, 617-618C 815 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 271 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92872 M 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 281, 288, 295, 302, 309, 316, 323C, 330C, 337C, 344C, 351C, 358C 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617-618C C92873 T 105 SKIN & PELAGE ALOPECIA 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 554, 561, 568, 575, 582 589, 596, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 540, 603 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 477, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 484, 491, 498, 505, 92, 99, 106 183, 190, 197 274, 281, 288 512, 519, 533 MASS(ES) AXILLARY REGION-LEFT WEEK 76 WEEK 79 526 547C, 554C, 561C, 568C 816 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 272 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92874 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 645, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 731C C92875 M 79 APPEARANCE MALOCCLUSION THIN 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 553 553 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 553 DISCHARGE NASAL RED IN COLOR 553 EXCRETION FEW FECES 547, 553 817 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 273 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92875 M 79 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 505, 519, 526, 533, 540, 547, 553 YELLOW HAIR COAT 553 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C92876 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 365-366C SURGERY PERFORMED BY PATTIE MURPHY 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 818 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 274 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92877 T 105 APPEARANCE MALOCCLUSION 99 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701, 57, 64, 71, 78, 85, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 708, 715, 722, 729, 92, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 732 C92878 M 81 APPEARANCE MALOCCLUSION THIN 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561-562 519, 526, 562 EYES DISCOLORED/LIGHT RED EYE-LEFT 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C 819 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 275 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92878 M 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561-562C VENTRAL-THORACIC WEEK 45 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561-562C C92879 M 93 APPEARANCE HEAD TILT LEFT HUNCHED THIN 645 603, 610 596, 603, 610, 617, 624, 631, 638, 645 BEHAVIOR ATAXIC <UNSPECIFIED> 638, 645 EYES RED DISCHARGE EYES 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 351, 358, 365, 435, 442 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 820 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 276 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92879 M 93 MASS(ES) AXILLARY REGION-LEFT WEEK 69 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C INGUINAL REGION-LEFT WEEK 65 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 526C, 533C, 540C, 547C, 554C, 561, 568 INGUINAL REGION-RIGHT WEEK 50 344C VENTRAL-ABDOMINAL-RIGHT WEEK 65 449, 456, 463, 470, 477C, 484C, 491C RECTUM WEEK 92 638C, 645C C92880 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92881 T 105 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN DIGIT(S)-HIND-LEFT 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 733 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 733 722 821 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 277 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92881 T 105 EXCRETION FEW FECES 526 EYES DISCOLORED/LIGHT RED EYE-RIGHT 414 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 INGUINAL REGION-LEFT WEEK 99 666, 673, 680, 687, 694, 701 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C C92882 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 69 AXILLARY REGION-RIGHT WEEK 100 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 733C 694C, 701C, 708C, 715C, 722C, 729C, 733C 822 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 278 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92883 M 87 APPEARANCE MALOCCLUSION 64, 71, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 99, 106, 113, 120, 127, 134, 141, 148, 155 190, 197, 204, 211, 218, 225, 232, 239, 246 281, 288, 295, 302, 309, 316, 323, 330, 337 372, 379, 386, 393, 400, 407, 414, 421, 428 463, 470, 477, 484, 491, 498, 505, 512, 519 554, 561, 568, 575, 582, 589, 596, 603-604 BEHAVIOR ATAXIC <UNSPECIFIED> 603-604 DISCHARGE NASAL RED IN COLOR 64 EXCRETION NO FECES 604 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 INGUINAL REGION-RIGHT WEEK 75 561, 568 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603-604C 823 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 279 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92884 M 96 APPEARANCE CONVULSIONS THIN 652 624, 631, 638, 645, 652, 659, 666 EXCRETION FEW FECES 624, 631 EYES PROTRUDING EYES 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 652, 659, 666 SKIN & PELAGE PALE ENTIRE BODY 666 SORE/SCAB AXILLARY REGION-RIGHT 666 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 73 WEEK 89 AXILLARY REGION-RIGHT WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 79 505C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 589C, 596C 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617C, 624C 824 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 280 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92884 M 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 79 631C, 638C, 645C, 652C, 659C, 666C VENTRAL-ABDOMINAL-RIGHT WEEK 65 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C WEEK 74 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C C92885 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 134, 141, 225, 232, 316, 323, 407, 414, 498, 505, 589, 596, 680 22, 29, 36, 43, 50, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 57, 64, 71, 78, 85, 176, 183, 190, 197, 267, 274, 281, 288, 358, 365, 372, 379, 449, 456, 463, 470, 540, 547, 554, 561, 631, 638, 645, 652, 92, 120, 127 204, 211, 218 295, 302, 309 386, 393, 400 477, 484, 491 568, 575, 582 659, 666, 673 MASS(ES) INGUINAL REGION-LEFT WEEK 99 VENTRAL-ABDOMINAL WEEK 15 687, 694, 701, 708C, 715C, 722C, 729C, 733C 99, 106, 113 825 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 281 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92886 M 81 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN 554, 561 561 EYES DISCOLORED/LIGHT RED EYES RED DISCHARGE EYE-RIGHT 554, 561 526 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL SORE/SCAB AXILLARY REGION-LEFT 561 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 59 INGUINAL REGION-RIGHT WEEK 59 407, 414, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C 826 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 282 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92887 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92888 M 79 APPEARANCE HEAD TILT RIGHT 551, 553 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 553 EXCRETION FEW FECES 553 EYES RED DISCHARGE EYE-RIGHT 519, 526, 533, 540, 547, 551, 553 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 827 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 283 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92889 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY PATTIE MURPHY 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92890 T 105 APPEARANCE MALOCCLUSION 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 828 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 284 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92891 M 85 APPEARANCE HEAD TILT LEFT THIN 582, 589, 591 591 BEHAVIOR RECUMBENT LATERAL 591 EXCRETION FEW FECES 591 RESPIRATION AUDIBLE 591 IRREGULAR 591 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 829 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 285 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92892 T 105 APPEARANCE OBES 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92893 M 88 APPEARANCE HUNCHED MALOCCLUSION THIN 610 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 337, 344, 351, 358 BEHAVIOR SENSITIVE TO TOUCH 337 SKIN & PELAGE ALOPECIA ROUGH HAIR COAT SORE/SCAB VENTRAL-THORACIC-LEFT 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463 393, 400, 407, 470, 477, 484, 491, 498, 505, 512, 519, 526, 554 603, 610 830 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 286 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92893 M 88 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 INGUINAL REGION-RIGHT WEEK 76 VENTRAL-ABDOMINAL-RIGHT WEEK 76 VENTRAL-THORACIC-LEFT WEEK 74 RECTUM WEEK 61 512, 519C, 526C, 533C, 540C, 547, 554, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C 526C, 533C, 540C, 547C, 554C 526C, 533C, 540C, 547C, 554C, 561C, 568C 512, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C 421C, 428C, 435C, 442C, 449C, 456C, 463C C92894 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 831 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 287 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92895 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 87 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C C92896 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 VENTRAL-ABDOMINAL-RIGHT WEEK 97 VENTRAL-THORACIC WEEK 104 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 673C, 680C, 687C, 694C, 701C, 708C, 715C 722, 729, 731 832 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 288 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92896 T 105 WART LIKE LESION(S) MOUTH WEEK 46 316 C92897 T 105 APPEARANCE THIN 722, 729-731 EXCRETION FEW FECES 730-731 EYES SQUINTED PERI-ORBITAL 687, 730-731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 694, 701, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C92898 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 833 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 289 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92899 3 53 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 365-366 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 SURGERY PERFORMED BY JOHN VAN BERKUM 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92900 D 72 APPEARANCE MALOCCLUSION MISSING TEETH 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 504 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 449 456, 463, 470, 477, 484, 491, 498, 504 EXCRETION NONFORMED FECES 225 834 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 290 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92900 D 72 EYES CLEAR DISCHARGE EYE-LEFT 393 SKIN & PELAGE YELLOW HAIR COAT 85 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 C92901 M 79 APPEARANCE HEAD TILT LEFT 533, 540, 547, 551 BEHAVIOR ATAXIC <UNSPECIFIED> 551 DISCHARGE NASAL RED IN COLOR 551 EXCRETION FEW FECES 526, 533, 540, 547, 551 EYES RED DISCHARGE EYE-LEFT 547, 551 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 519, 526, 533, 540 835 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 291 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92901 M 79 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 414, 470, 477, 484, 491, 498, 505, 512 MASS(ES) AXILLARY REGION-LEFT WEEK 48 AXILLARY REGION-RIGHT WEEK 48 VENTRAL-ABDOMINAL WEEK 61 RECTUM WEEK 76 330 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 421, 428, 435, 442, 449, 456, 463 526C, 533C, 540C, 547C, 551C C92902 D 89 APPEARANCE OBES 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 610, 617 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 836 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 292 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92903 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92904 M 101 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT 610 VENTRAL-THORACIC 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 69 INGUINAL REGION-RIGHT WEEK 70 VENTRAL-ABDOMINAL-LEFT WEEK 97 VENTRAL-CERVICAL-LEFT WEEK 63 477, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701-702C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701-702C 673C, 680C, 687C, 694C, 701-702C 435, 442, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603, 610, 617, 624, 631, 638, 645 837 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 293 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92904 M 101 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701-702C WART LIKE LESION(S) MOUTH WEEK 89 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701-702 C92905 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92906 T 105 APPEARANCE MALOCCLUSION 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 731 EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYES 519, 526 610, 617 SKIN & PELAGE SORE/SCAB MOUTH 631, 638, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 838 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 294 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92906 T 105 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 498, 505, 512, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 624 MASS(ES) AXILLARY REGION-LEFT WEEK 95 AXILLARY REGION-RIGHT WEEK 70 WEEK 95 VENTRAL-ABDOMINAL-RIGHT WEEK 65 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 731C 484, 491 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 731C 449, 456, 463, 470, 477, 484, 491 C92907 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 733 839 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92908 M 93 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL 631, 646 631, 646 646 EXCRETION FEW FECES NO FECES 638, 645-646 624, 631 EYES RED DISCHARGE EYES 624, 631 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-CERVICAL WEEK 78 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C, 631C, 638C, 645-646C 840 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 296 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92909 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C C92910 3 53 APPEARANCE MALOCCLUSION 108, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 DISCHARGE NASAL RED IN COLOR 108, 113 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 841 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 297 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92911 T 105 APPEARANCE MALOCCLUSION 582, 589, 596, 603, 631, 638, 645, 652, 673, 680, 687, 694, 715 722, 729, 732 EYES CLEAR DISCHARGE EYE-LEFT 526 SKIN & PELAGE ROUGH HAIR COAT 239 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 302, 309, 393, 400, 610, 617, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 246, 316, 323, 330, 337, 407, 421, 428, 435, 624, 659, 666, 701, 57, 64, 71, 78, 85, 155, 162, 169, 176, 253, 260, 267, 274, 344, 351, 358, 365, 442, 470, 547, 554, 708 92, 99, 106 183, 190, 197 281, 288, 295 372, 379, 386 561, 568, 575 MASS(ES) AXILLARY REGION-LEFT WEEK 69 AXILLARY REGION-RIGHT WEEK 91 VENTRAL-ABDOMINAL WEEK 65 VENTRAL-ABDOMINAL-RIGHT WEEK 60 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533, 540 631, 638, 645 449, 456, 463 414 842 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 298 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92912 T 105 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB VENTRAL-ABDOMINAL 645 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 554, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 732C C92913 T 105 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-ABDOMINAL 715, 722 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 843 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 299 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92913 T 105 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 631, 638, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 731C C92914 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92915 M 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 670 EXCRETION FEW FECES NO FECES 645, 659 670 EYES RED DISCHARGE EYES 670 SKIN & PELAGE SORE/SCAB PAWS-HIND 652, 659, 666, 670 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 844 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 300 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92915 M 96 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 575, 582 589, 596, 603, 610, 617, 624, 631, 638 MASS(ES) AXILLARY REGION-LEFT WEEK 80 554, 561, 568 C92916 M 93 APPEARANCE HEAD TILT RIGHT MALOCCLUSION 617, 624, 631, 638, 645, 649 22 EXCRETION FEW FECES 624, 631, 638 EYES PROTRUDING EYES 519, 526, 533 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT LATERAL-THORACIC-LEFT PAW-HIND-RIGHT SHOULDER-LEFT 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 589, 596, 603, 610, 617, 624, 631, 638, 645, 649 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 845 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 301 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92916 M 93 NORMAL NO REMARKABLE OBSERVATIONS 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253 MASS(ES) AXILLARY REGION-LEFT WEEK 48 WEEK 70 AXILLARY REGION-RIGHT WEEK 46 INGUINAL REGION-LEFT WEEK 84 INGUINAL REGION-RIGHT WEEK 75 VENTRAL-ABDOMINAL-RIGHT WEEK 38 330 484, 491, 498, 505, 512, 519, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 649C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 649C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 649C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 649C 260, 267, 274, 281, 288, 295, 302, 309, 316 WART LIKE LESION(S) MOUTH WEEK 75 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 649 846 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 302 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92917 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C92918 M 73 APPEARANCE THIN 508 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 508 EXCRETION FEW FECES 504-505, 508 EYES RED DISCHARGE EYES 504-505, 508 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 379 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 232, 239, 323, 330, 421, 428, 22, 29, 36, 43, 50, 127, 134, 141, 148, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 57, 64, 71, 78, 85, 155, 162, 197, 204, 274, 281, 288, 295, 365, 372, 386, 393, 463, 470, 477, 484, 92, 99, 106 211, 218, 225 302, 309, 316 400, 407, 414 491, 498 847 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 303 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92918 M 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 169, 176, 183, 190 C92919 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92920 M 72 APPEARANCE HUNCHED 498 EXCRETION FEW FECES NO FECES 489, 491 498 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 351, 358, 442, 449, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 288, 295, 365, 372, 379, 386, 456, 463, 470, 477, 57, 64, 71, 78, 85, 155, 162, 169, 176, 302, 309, 316, 323, 393, 400, 407, 414, 484 92, 99, 106 183, 190, 197 330, 337, 344 421, 428, 435 MASS(ES) VENTRAL-ABDOMINAL WEEK 36 VENTRAL-ABDOMINAL-LEFT WEEK 34 246C, 253C, 260C, 267, 274, 281 232C, 239C, 246C, 253C, 260C, 267C, 274C, 281C 848 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 304 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92921 M 57 APPEARANCE THIN 372, 379, 386, 393 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 379, 386, 393 EXCRETION FEW FECES 337, 344, 351, 358, 365, 372, 379, 386, 393 EYES RED DISCHARGE EYES 372, 379, 386, 393 RESPIRATION LABORED 393 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT SORE/SCAB AXILLARY REGION-LEFT 365, 372, 379, 386, 393 365, 372, 379, 386, 393 379, 386, 393 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 323 849 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 305 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92921 M 57 MASS(ES) AXILLARY REGION-LEFT WEEK 48 330, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C HEAD-MAXILLARY-RIGHT WEEK 43 295, 302, 309, 316 WART LIKE LESION(S) MOUTH WEEK 46 316 C92922 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C92923 3 53 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT 365-366 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 351, 358 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 330, 337, 344 850 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 306 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92923 3 53 SURGERY PERFORMED BY PATTIE MURPHY 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C92924 M 85 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 232, 239, 323, 330, 414, 421, 505, 512, 22, 29, 36, 43, 50, 127, 134, 141, 148, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 57, 64, 71, 78, 85, 155, 162, 169, 176, 274, 281, 288, 295, 365, 372, 379, 386, 456, 463, 470, 477, 547, 554, 561, 568 92, 99, 106 183, 190, 197 302, 309, 316 393, 400, 407 484, 491, 498 MASS(ES) LIMB-HIND-LEFT WEEK 83 VENTRAL-ABDOMINAL WEEK 30 575C, 582C, 589C 204, 211, 218, 225 C92925 T 105 EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYE-RIGHT 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 722, 729, 733 851 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 307 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92925 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-RIGHT WEEK 96 WEEK 99 INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL WEEK 92 666 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 733C 638, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729C, 733C C92926 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92927 T 105 EYES PROTRUDING EYES 519, 526, 533 SKIN & PELAGE BLACK SKIN VENTRAL-THORACIC ROUGH HAIR COAT 729, 731 554 852 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 308 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92927 T 105 SKIN & PELAGE SORE/SCAB DORSAL 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 DORSAL-THORACIC 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 512, 540, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 547, 561 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 470, 477, 484, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 491, 498, 505 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 INGUINAL REGION-LEFT WEEK 105 INGUINAL REGION-RIGHT WEEK 94 VENTRAL-CERVICAL WEEK 63 WEEK 82 VENTRAL-THORACIC-RIGHT WEEK 91 610, 617, 624, 631, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 729C, 731C 652, 659, 666, 673, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 731C 435C, 442C, 449C, 456C, 463C 568, 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 731C 853 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 309 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92928 M 83 BEHAVIOR ATAXIC <UNSPECIFIED> 575 HYPOACTIVE <UNSPECIFIED> 575 EXCRETION FEW FECES 575 NO FECES 572 EYES RED DISCHARGE EYE-RIGHT EYES 505, 512, 519, 533, 540, 547, 554, 561 526, 568, 572, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92929 M 60 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414-415 351, 358, 365, 372, 379, 386, 393, 400, 407, 414-415 372, 379, 386, 393, 400, 407, 414-415 BEHAVIOR CIRCLING RIGHT 358 854 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 310 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F1 DOSE: 0 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92929 M 60 EXCRETION FEW FECES 337 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 C92930 3 53 APPEARANCE MALOCCLUSION 176, 183 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT 365-366 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358 SURGERY PERFORMED BY JOHN VAN BERKUM 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 855 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 311 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92865 F1 281 41 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 15 X 15MM C92867 F1 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 104 10 X 10MM 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 104 15 X 15MM C92871 F1 652 94 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 94 40 X 40MM 687 99 TO 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 94 50 X 40MM 701 101 TO 715 103 MASS(ES) INGUINAL REGION-LEFT WEEK 94 40 X 40MM 722 104 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 94 50 X 50MM 526 76 TO 540 78 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 25 X 20MM 856 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 312 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92871 F1 547 79 TO 582 84 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 30 X 40MM 589 85 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 40 X 50MM 596 86 TO 659 95 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 35 X 40MM 666 96 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 45 X 50MM 673 97 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 50 X 50MM 680 98 TO 694 100 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 60 X 50MM 701 101 TO 731 105 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 50 X 50MM C92872 F1 596 86 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 83 10 X 10MM 857 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 313 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92872 F1 617 89 TO 618 89 MASS(ES) INGUINAL REGION-LEFT WEEK 83 30 X 20MM 323 47 TO 330 48 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 25 X 20MM 337 49 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 30 X 25MM 344 50 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 30 X 25MM 351 51 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 30 X 30MM 358 52 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 35 X 30MM 365 53 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 40 X 35MM 400 58 TO 414 60 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 55 X 45MM 858 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 314 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92872 F1 421 61 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 55 X 50MM 442 64 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 55 X 60MM 449 65 TO 470 68 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 70 X 65MM 477 69 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 60 X 65MM 512 74 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 80 X 70MM 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 70 X 70MM 561 81 TO 618 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 80 X 70MM C92873 F1 547 79 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 79 10 X 10MM 859 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 315 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92874 F1 652 94 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 10 X 10MM 666 96 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 15 X 15MM 680 98 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 20 X 20MM 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 25 X 25MM 722 104 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 40 X 30MM C92876 F1 365 53 TO 366 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 10 X 10MM C92878 F1 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 10 X 10MM 428 62 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 15 X 15MM 860 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 316 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92878 F1 470 68 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 30 X 30MM 477 69 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 20 X 20MM 498 72 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 30 X 20MM 512 74 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 35 X 30MM 526 76 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 35 X 35MM 561 81 TO 562 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 35 X 35MM 309 45 MASS(ES) VENTRAL-THORACIC WEEK 45 20 X 20MM 316 46 TO 330 48 MASS(ES) VENTRAL-THORACIC WEEK 45 30 X 30MM 861 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 317 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92878 F1 337 49 TO 351 51 MASS(ES) VENTRAL-THORACIC WEEK 45 35 X 30MM 358 52 TO 393 57 MASS(ES) VENTRAL-THORACIC WEEK 45 45 X 40MM 400 58 TO 421 61 MASS(ES) VENTRAL-THORACIC WEEK 45 55 X 60MM 428 62 TO 442 64 MASS(ES) VENTRAL-THORACIC WEEK 45 60 X 60MM 449 65 TO 505 73 MASS(ES) VENTRAL-THORACIC WEEK 45 75 X 70MM 512 74 TO 519 75 MASS(ES) VENTRAL-THORACIC WEEK 45 90 X 80MM 526 76 TO 533 77 MASS(ES) VENTRAL-THORACIC WEEK 45 100 X 85MM 540 78 TO 554 80 MASS(ES) VENTRAL-THORACIC WEEK 45 90 X 80MM 862 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 318 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92878 F1 561 81 TO 562 81 MASS(ES) VENTRAL-THORACIC WEEK 45 100 X 100MM C92879 F1 477 69 TO 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 512 74 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 69 15 X 15MM 603 87 TO 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 449 65 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 65 10 X 10MM 512 74 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 65 15 X 15MM 344 50 MASS(ES) INGUINAL REGION-RIGHT WEEK 50 10 X 10MM 477 69 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 10 X 10MM 863 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 319 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92879 F1 638 92 TO 645 93 MASS(ES) RECTUM WEEK 92 15 X 15MM C92881 F1 687 99 TO 722 104 MASS(ES) INGUINAL REGION-LEFT WEEK 99 10 X 10MM 729 105 TO 733 105 MASS(ES) INGUINAL REGION-LEFT WEEK 99 20 X 20MM C92882 F1 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 69 20 X 20MM 484 70 TO 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 69 20 X 25MM 498 72 TO 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 69 30 X 30MM 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 69 30 X 40MM 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 69 45 X 40MM 864 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 320 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92882 F1 526 76 TO 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 69 50 X 40MM 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 69 50 X 50MM 561 81 TO 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 69 50 X 60MM 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 69 60 X 65MM 603 87 TO 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 69 60 X 50MM 617 89 MASS(ES) AXILLARY REGION-LEFT WEEK 69 75 X 60MM 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 69 75 X 60MM 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 69 75 X 60MM 865 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 321 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92882 F1 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 69 75 X 60MM 645 93 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 69 70 X 65MM 666 96 TO 733 105 MASS(ES) AXILLARY REGION-LEFT WEEK 69 80 X 65MM 694 100 TO 733 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 10 X 10MM C92883 F1 519 75 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 10 X 10MM 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 15 X 15MM 589 85 TO 604 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 20 X 20MM C92884 F1 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 73 10 X 10MM 866 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 322 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92884 F1 617 89 TO 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 89 15 X 15MM 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 89 25 X 20MM 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 89 15 X 15MM 666 96 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 89 20 X 15MM 589 85 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 10 X 10MM 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 79 15 X 15MM 631 91 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 79 20 X 20MM 666 96 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 79 30 X 20MM 867 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 323 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92884 F1 449 65 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 10 X10MM 505 73 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 15 X 15MM 526 76 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 15 X 20MM 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 20 X 25MM 561 81 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 25 X 30MM 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 30 X 20MM POSTERIOR 519 75 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 20 X 15MM POSTERIOR 547 79 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 20 X 20MM POSTERIOR 868 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 324 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92884 F1 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 30 X 30MM POSTERIOR 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 20 X 20MM POSTERIOR 617 89 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 25 X 25MM POSTERIOR 666 96 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 30 X 25MM POSTERIOR C92885 F1 708 102 TO 715 103 MASS(ES) INGUINAL REGION-LEFT WEEK 99 10 X 10MM 722 104 TO 733 105 MASS(ES) INGUINAL REGION-LEFT WEEK 99 15 X 15MM C92886 F1 421 61 TO 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 59 15 X 15MM 435 63 TO 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 59 20 X 15MM 869 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 325 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92886 F1 449 65 TO 470 68 MASS(ES) AXILLARY REGION-LEFT WEEK 59 25 X 20MM 477 69 TO 533 77 MASS(ES) AXILLARY REGION-LEFT WEEK 59 10 X 10MM 407 59 TO 463 67 MASS(ES) INGUINAL REGION-RIGHT WEEK 59 15 X 15MM C92893 F1 519 75 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 10 X 10MM 561 81 TO 610 88 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 10 X 10MM 526 76 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 10 X 10MM 526 76 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 10 X 10MM 519 75 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 10 X 10MM 870 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 326 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92893 F1 526 76 TO 540 78 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 15 X 15MM 547 79 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 10 X 10MM 554 80 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 15 X 15MM 561 81 TO 575 83 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 25 X 25MM 582 84 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 30 X 30MM 589 85 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 40 X 40MM 596 86 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 40 X 45MM 603 87 TO 610 88 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 40 X 40MM 871 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 327 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92893 F1 421 61 MASS(ES) RECTUM WEEK 61 15 X 20MM 428 62 MASS(ES) RECTUM WEEK 61 15 X 20MM 435 63 TO 463 67 MASS(ES) RECTUM WEEK 61 15 X 20MM C92895 F1 603 87 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 87 10 X 10MM 638 92 TO 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 87 15 X 15MM 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 87 20 X 15MM 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 87 25 X 25MM 666 96 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 87 30 X 25MM 872 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 328 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92895 F1 680 98 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 87 40 X 30MM 708 102 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 87 45 X 40MM C92896 F1 526 76 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 20 X 15MM 561 81 TO 589 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 25 X 20MM 596 86 TO 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 30 X 25MM 617 89 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 35 X 30MM 631 91 TO 715 103 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 50 X 35MM 722 104 TO 731 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 60 X 40MM 873 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 329 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92896 F1 673 97 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 20 X 15MM 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 30 X 30MM C92901 F1 526 76 TO 551 79 MASS(ES) RECTUM WEEK 76 10 X 10MM C92904 F1 484 70 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 69 15 X 15MM 512 74 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 69 30 X 25MM 547 79 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 69 30 X 30MM 561 81 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 69 35 X 35MM 874 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 330 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92904 F1 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 69 40 X 40MM 596 86 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 69 35 X 45MM 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 69 40 X 45MM 617 89 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 69 50 X 50MM 645 93 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 69 50 X 55MM 666 96 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 69 50 X 60MM 680 98 TO 702 101 MASS(ES) AXILLARY REGION-LEFT WEEK 69 55 X 65MM 484 70 TO 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 10 X 10MM 875 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 331 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92904 F1 519 75 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 15 X 10MM 526 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 10MM 533 77 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 10MM 540 78 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 10MM 547 79 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 15MM 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 15MM 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 20MM 568 82 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 20MM 876 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 332 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92904 F1 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 20MM 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 20MM 589 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 20MM 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 20MM 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 35 X 30MM 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 35 X 30MM 617 89 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 877 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 333 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92904 F1 631 91 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 638 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 645 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 652 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 659 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 673 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 878 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 334 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92904 F1 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 694 100 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 701 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 702 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 702 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 673 97 TO 702 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 97 30 X 20MM 449 65 TO 463 67 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 10 X 10MM 470 68 TO 498 72 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 15 X 15MM 879 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 335 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92904 F1 505 73 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 20 X 20MM 512 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 30 X 30MM 519 75 TO 533 77 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 25 X 25MM 540 78 TO 596 86 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 10 X 10MM 505 73 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 10 X 10MM 512 74 TO 540 78 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 20 X 20MM 547 79 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 20 X 30MM 554 80 TO 575 83 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 30 X 35MM 880 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 336 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92904 F1 582 84 TO 589 85 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 40 X 40MM 596 86 TO 624 90 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 30 X 25MM 631 91 TO 702 101 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 30 X 35MM C92906 F1 659 95 TO 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 95 15 X 15MM 722 104 TO 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 95 20 X 20MM 659 95 TO 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 95 20 X 20MM 687 99 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 95 30 X 25MM 715 103 TO 731 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 95 40 X 30MM 881 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 337 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92908 F1 540 78 TO 554 80 MASS(ES) VENTRAL-CERVICAL WEEK 78 10 X 10MM 561 81 TO 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 78 15 X 15MM 589 85 TO 646 93 MASS(ES) VENTRAL-CERVICAL WEEK 78 20 X 20MM C92909 F1 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 20 X 20MM 687 99 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 30 X 20MM 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 45 X 30MM 722 104 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 80 X 45MM C92911 F1 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 882 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 338 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92911 F1 484 70 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 69 15 X 15MM 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 512 74 TO 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 69 20 X 20MM 526 76 MASS(ES) AXILLARY REGION-LEFT WEEK 69 15 X 15MM C92912 F1 561 81 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 10 X 10MM 575 83 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 30 X 20MM 596 86 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 30 X 30MM 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 40 X 45MM 883 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 339 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92912 F1 631 91 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 70 X 50MM 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 60 X 50MM 652 94 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 60 X 60MM 666 96 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 70 X 60MM 687 99 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 70 X 70MM 708 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 70 X 90MM 715 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 90 X 70MM 722 104 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 90 X 100MM 884 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 340 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92913 F1 645 93 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 10 X 10MM 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 15 X 15MM 666 96 TO 694 100 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 25 X 20MM 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 30 X 30MM 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 40 X 40MM 722 104 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 50 X 45MM C92916 F1 526 76 TO 649 93 MASS(ES) AXILLARY REGION-LEFT WEEK 70 10 X 10MM 316 46 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 20 X 20MM 885 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 341 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92916 F1 323 47 TO 330 48 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 25 X 20MM 337 49 TO 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 20 X 20MM 414 60 TO 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 25 X 20MM 449 65 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 60 X 45MM 519 75 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 25 X 25MM 554 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 35 X 35MM 561 81 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 60 X 50MM 610 88 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 50 X 40MM 886 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 342 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92916 F1 631 91 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 30 X 20MM 645 93 TO 649 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 15 X 15MM 582 84 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 84 15 X 15MM 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 84 20 X 20MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 84 15 X 15MM 610 88 TO 624 90 MASS(ES) INGUINAL REGION-LEFT WEEK 84 20 X 20MM 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 84 10 X 10MM 638 92 TO 649 93 MASS(ES) INGUINAL REGION-LEFT WEEK 84 20 X 20MM 887 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 343 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92916 F1 519 75 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 10 X 10MM 582 84 TO 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 15 X 15MM 603 87 TO 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 20 X 20MM 617 89 TO 649 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 50 X 40MM C92920 F1 246 36 TO 260 38 MASS(ES) VENTRAL-ABDOMINAL WEEK 36 10 X 10MM 232 34 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 25 X 20MM 239 35 TO 281 41 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 30 X 35MM C92921 F1 337 49 TO 358 52 MASS(ES) AXILLARY REGION-LEFT WEEK 48 10 X 10MM 888 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 344 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92921 F1 365 53 TO 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 48 15 X 15MM C92924 F1 575 83 MASS(ES) LIMB-HIND-LEFT WEEK 83 45 X 40MM 582 84 MASS(ES) LIMB-HIND-LEFT WEEK 83 60 X 50MM 589 85 TO 589 85 MASS(ES) LIMB-HIND-LEFT WEEK 83 80 X 60MM C92925 F1 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 10 X 10MM 694 100 TO 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 15 X 15MM 722 104 TO 733 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 20 X 20MM 582 84 TO 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 40 X 30MM 889 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 345 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92925 F1 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 40 X 35MM 617 89 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 60 X 40MM 631 91 TO 659 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 60 X 50MM 666 96 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 65 X 50MM 687 99 TO 715 103 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 70 X 60MM 722 104 TO 733 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 80 X 80MM 645 93 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL WEEK 92 10 X 10MM 687 99 TO 733 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 92 15 X 15MM 890 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 346 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92927 F1 638 92 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 10 X 10MM 673 97 TO 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 20 X 20MM 722 104 TO 731 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 40 X 40MM 729 105 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 105 15 X 15MM 680 98 TO 715 103 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 15 X 10MM 722 104 TO 731 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 25 X 20MM 435 63 TO 463 67 MASS(ES) VENTRAL-CERVICAL WEEK 63 10 X 10MM 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 82 10 X 10MM 891 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 347 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92927 F1 589 85 TO 659 95 MASS(ES) VENTRAL-CERVICAL WEEK 82 20 X 20MM 666 96 TO 673 97 MASS(ES) VENTRAL-CERVICAL WEEK 82 25 X 25MM 680 98 TO 715 103 MASS(ES) VENTRAL-CERVICAL WEEK 82 30 X 30MM 631 91 TO 645 93 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 91 20 X 20MM 652 94 TO 673 97 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 91 30 X 30MM 680 98 TO 715 103 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 91 40 X 35MM 722 104 TO 731 105 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 91 80 X 50MM MASSES APPEAR TO HAVE GROWN TOGETHER 892 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 348 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92931 D 70 EXCRETION FEW FECES 489-490 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 477C, 484C, 489-490C C92932 M 41 APPEARANCE THIN 281, 285 EXCRETION FEW FECES 281, 285 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-LEFT 281, 285 285 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113 893 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 349 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92932 M 41 MASS(ES) DORSAL WEEK 29 197C, 204C, 211C, 218C, 225C, 232C, 239C, 246C, 253C, 260C, 267C 274C VENTRAL-ABDOMINAL-LEFT WEEK 18 120C, 127C, 134C, 141C, 148C, 155C, 162C, 169C, 176C, 183C, 190C 197C, 204C, 211C, 218C, 225C, 232C, 239C, 246C, 253C, 260C, 267C 274C, 281C, 285C VENTRAL-ABDOMINAL-RIGHT WEEK 25 169C, 176C, 183C, 190C, 197C, 204C, 211C, 218C, 225C, 232C, 239C 246C, 253C, 260C, 267C, 274C, 281C, 285C VENTRAL-THORACIC-LEFT WEEK 25 169C, 176C, 183C, 190C C92933 M 92 APPEARANCE MALOCCLUSION 281, 316, 344, 358, 407, 414, 435, 442, 449, 456, 463, 470, 477 505, 512, 519, 540, 547, 554, 561, 568, 575, 582, 589, 631, 638 642 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 642 COLD TO TOUCH ENTIRE BODY 642 EXCRETION FEW FECES 642 NO FECES 642 EYES PALE EYES 642 894 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 350 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92933 M 92 EYES PROTRUDING EYES 519 RESPIRATION LABORED 642 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-RIGHT SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 642 638, 642 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 288, 295 302, 309, 323, 330, 337, 351, 421, 428 MASS(ES) AXILLARY REGION-LEFT WEEK 64 INGUINAL REGION-RIGHT WEEK 74 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 642C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 642C 895 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 351 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92934 M 87 APPEARANCE HEAD TILT RIGHT MALOCCLUSION MISSING TEETH THIN 596, 603 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603 575, 582, 589, 596, 603 596, 603 BEHAVIOR ATAXIC <UNSPECIFIED> 603 EYES PROTRUDING EYES 519 RESPIRATION LABORED 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 896 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 352 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92935 M 88 APPEARANCE HUNCHED 603, 610, 612 BEHAVIOR ATAXIC <UNSPECIFIED> 610, 612 EXCRETION FEW FECES 575, 582, 610, 612 EYES RED DISCHARGE EYE-RIGHT 603, 610, 612 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 505 ROUGH HAIR COAT 603 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 498, 512, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 519, 526, 533, 540, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 470, 547, 554 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 SHOULDER-RIGHT WEEK 65 561C, 568C, 575C, 582C, 589C, 596C, 603C 449C, 456C, 463C 897 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 353 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92936 T 105 EXCRETION FEW FECES 722, 729, 732 EYES RED DISCHARGE EYES 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 63 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C C92937 M 65 APPEARANCE HUNCHED MALOCCLUSION THIN 449-450 400, 407, 414, 421, 428, 435, 442, 449-450 449-450 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 450 EXCRETION FEW FECES 393 898 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 354 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92937 M 65 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 MASS(ES) AXILLARY REGION-LEFT WEEK 64 VENTRAL-ABDOMINAL WEEK 61 VENTRAL-ABDOMINAL-LEFT WEEK 43 442, 449-450 421, 428C, 435C, 442C, 449-450C 295C, 302C, 309C, 316C C92938 T 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT 722, 729, 732 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 899 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 355 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92939 M 71 SKIN & PELAGE BLACK SKIN VENTRAL-CERVICAL-RIGHT 484 SORE/SCAB VENTRAL-THORACIC 491 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316 MASS(ES) AXILLARY REGION-LEFT WEEK 58 VENTRAL-ABDOMINAL-RIGHT WEEK 63 VENTRAL-CERVICAL-RIGHT WEEK 47 400, 407, 414, 421, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C C92940 M 87 APPEARANCE THIN 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337 900 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 356 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92940 M 87 MASS(ES) AXILLARY REGION-LEFT WEEK 50 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C INGUINAL REGION-LEFT WEEK 73 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C VENTRAL-THORACIC-RIGHT WEEK 76 526C C92941 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92942 M 50 SKIN & PELAGE BLACK SKIN DORSAL SORE/SCAB DORSAL 323 330, 337, 344, 348 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64 MASS(ES) DORSAL WEEK 11 VENTRAL-ABDOMINAL-RIGHT WEEK 50 71C, 78C, 85C, 92C, 99C, 106C, 113C, 120C, 127C, 134C, 141C 148C, 155C, 162C, 169C, 176C, 183C, 190C, 197C, 204C, 211C, 218C 225C, 232C, 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C 302C, 309C, 316C, 323C, 330C, 337C, 344C, 348C 348C 901 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 357 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92943 M 97 APPEARANCE HEAD TILT LEFT THIN 631, 638, 645, 652, 659, 666, 673, 677 673, 677 EXCRETION FEW FECES 645, 677 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 638C, 645C, 652C, 659C, 666C, 673C, 677C C92944 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92945 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 902 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 358 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92946 T 105 APPEARANCE PROLAPSED VAGINA 393, 400, 407, 421, 428 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 414, 512, 519, 610, 617, 701, 708, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 435, 442, 449, 456, 526, 533, 547, 554, 624, 631, 638, 645, 715, 722 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 463, 470, 477, 484, 561, 568, 575, 582, 652, 659, 666, 673, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 491, 498, 505 589, 596, 603 680, 687, 694 MASS(ES) AXILLARY REGION-RIGHT WEEK 105 VENTRAL-ABDOMINAL WEEK 78 729, 732 540 C92947 M 94 APPEARANCE MALOCCLUSION THIN 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 656 656 EXCRETION FEW FECES 656 903 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 359 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92947 M 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 C92948 M 99 APPEARANCE THIN 673, 691 EXCRETION FEW FECES 669, 673, 680, 691 RESPIRATION IRREGULAR 669 SKIN & PELAGE BLUE SKIN PAWS-ALL TAIL ROUGH HAIR COAT 669, 673 669, 673 687, 691 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 MASS(ES) VENTRAL-CERVICAL WEEK 96 666, 669C, 673C, 680C, 687C, 691C 904 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 360 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92949 M 33 SKIN & PELAGE SORE/SCAB LATERAL-CERVICAL-RIGHT 8 VENTRAL-CERVICAL-LEFT 229 NORMAL NO REMARKABLE OBSERVATIONS 1, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 3 WEEK 21 190C, 197C, 204C, 211C, 218C, 225C, 229C 141C, 148C, 155C, 162C, 169C, 176C, 183C C92950 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92951 M 103 APPEARANCE MALOCCLUSION MISSING TEETH 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386 DISCHARGE GENITAL RED IN COLOR 491, 519 905 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 361 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92951 M 103 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 INGUINAL REGION-LEFT WEEK 64 VENTRAL-ABDOMINAL-RIGHT WEEK 80 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C 554, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C C92952 M 66 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 456 MODERATE 456 EXCRETION FEW FECES 456 906 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 362 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92952 M 66 EYES RED DISCHARGE EYES 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 421, 428 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 351, 358, 365, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 MASS(ES) AXILLARY REGION-LEFT WEEK 50 WEEK 57 WEEK 63 344 393, 400, 407, 414 435C, 442C, 449C, 456C C92953 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 733 907 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 363 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92954 M 101 EXCRETION NO FECES 701, 704 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE HEAD-CRANIAL YELLOW HAIR COAT 505, 512, 519, 526, 533, 540, 547, 701, 704 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 704 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498 MASS(ES) INGUINAL REGION-LEFT WEEK 76 VENTRAL-CERVICAL-RIGHT WEEK 77 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 704C C92955 M 92 APPEARANCE THIN 633, 638 BEHAVIOR ATAXIC <UNSPECIFIED> 638 908 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 364 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92955 M 92 EXCRETION FEW FECES 633 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB DORSAL-CERVICAL SHOULDER-RIGHT YELLOW HAIR COAT 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 633, 638 463, 470, 477, 484, 491, 498 633, 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 435, 442, 449, 456, 505 MASS(ES) INGUINAL REGION-LEFT WEEK 75 INGUINAL REGION-RIGHT WEEK 76 WEEK 83 VENTRAL-ABDOMINAL WEEK 62 VENTRAL-ABDOMINAL-RIGHT WEEK 84 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 633C, 638C 526 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 633C, 638C 428C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 633C, 638C 909 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 365 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92956 M 102 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT SORE/SCAB MOUTH VENTRAL-ABDOMINAL-LEFT 673, 680, 687, 694, 701, 708-709 638, 645 701, 708-709 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 90 INGUINAL REGION-LEFT WEEK 82 624C, 631 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708-709C C92957 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 910 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 366 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92957 T 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C WART LIKE LESION(S) NOSE WEEK 46 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498 C92958 M 89 APPEARANCE HEAD TILT RIGHT HUNCHED 610, 617, 619 603, 610, 617, 619 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 619 RECUMBENT LATERAL 619 EXCRETION FEW FECES 575, 582, 619 SKIN & PELAGE ROUGH HAIR COAT 603, 610, 617, 619 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 911 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 367 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92958 M 89 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 589, 596 C92959 M 98 APPEARANCE MALOCCLUSION MISSING TEETH 407, 413-414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680 EXCRETION FEW FECES 673 NO FECES 680 EYES RED DISCHARGE EYE-RIGHT 680 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB MOUTH 680 413-414, 421 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 912 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 368 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92959 M 98 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400 C92960 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 72 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 733C C92961 M 99 APPEARANCE HEAD TILT LEFT LIMITED USE LIMB-HIND-LEFT THIN 687, 691 687, 691 687, 691 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 687, 691 913 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 369 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92961 M 99 EXCRETION FEW FECES 691 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT SORE/SCAB PAW-HIND-RIGHT 687, 691 526, 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 260, 267, 274, 281, 288, 295, 302 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-LEFT WEEK 45 AXILLARY REGION-RIGHT WEEK 36 WEEK 58 INGUINAL REGION-LEFT WEEK 76 309, 316, 323 246, 253 400, 407, 414, 421 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 691C 914 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 370 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92962 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92963 M 94 APPEARANCE MALOCCLUSION 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 656 EYES PROTRUDING EYES 519 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-RIGHT PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 652, 656 656 656 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) AXILLARY REGION-LEFT WEEK 72 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 656C 915 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 371 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92963 M 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C VENTRAL-ABDOMINAL-LEFT WEEK 83 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 656C VENTRAL-ABDOMINAL-RIGHT WEEK 68 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 656C C92964 T 105 APPEARANCE MALOCCLUSION 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 731 EXCRETION FEW FECES 540 NONFORMED FECES 400 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 MASS(ES) VENTRAL-CERVICAL WEEK 83 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 916 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 372 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92964 T 105 MASS(ES) VENTRAL-CERVICAL WEEK 83 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 731C C92965 D 82 APPEARANCE HUNCHED THIN 570, 573 561, 568, 570, 573 EXCRETION NO FECES 570, 573 EYES RED DISCHARGE EYES 547, 554, 561, 568, 570, 573 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 570C, 573C 917 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 373 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92966 M 72 APPEARANCE HEAD TILT RIGHT THIN 502 498, 502 BEHAVIOR ATAXIC <UNSPECIFIED> 502 EYES RED DISCHARGE EYE-RIGHT 316 SKIN & PELAGE ROUGH HAIR COAT 498, 502 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 323, 330, 337, 407, 414, 421, 428, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 C92967 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 918 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 374 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92968 D 69 EXCRETION FEW FECES 470, 477 EYES DILATED PUPIL EYES RED DISCHARGE EYES 470, 477 470, 477 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 C92969 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92970 T 105 APPEARANCE MALOCCLUSION THIN 78 715, 722, 729, 731 DISCHARGE NASAL RED IN COLOR 722 EXCRETION FEW FECES 715, 722, 729, 731 919 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 375 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92970 T 105 EYES PROTRUDING EYES 519 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 582, 589, 22, 29, 36, 43, 50, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 526, 596, 603, 610, 617, 57, 64, 71, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 533, 540, 547, 554, 624, 631, 638, 645 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 561, 568, 575 C92971 M 90 APPEARANCE MALOCCLUSION MISSING TEETH THIN 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624 554, 561, 568, 575, 582, 589, 596, 603 624 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 617, 624 920 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 376 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92971 M 90 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 MASS(ES) INGUINAL REGION-LEFT WEEK 67 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C C92972 M 96 APPEARANCE THIN 666, 670 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 670 EXCRETION FEW FECES 670 EYES RED DISCHARGE EYE-RIGHT EYES 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666 670 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 365, 372, 379, 386, 393, 400, 407, 414 921 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 377 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92972 M 96 NORMAL NO REMARKABLE OBSERVATIONS 421, 428 MASS(ES) SHOULDER-RIGHT WEEK 72 VENTRAL-ABDOMINAL-RIGHT WEEK 48 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 670C 330, 337, 344, 351, 358 C92973 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92974 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 922 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 378 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92974 T 105 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 104 722, 729, 732 C92975 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 554, 561, 568 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 VENTRAL-ABDOMINAL-RIGHT WEEK 47 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 722C, 729C, 733C 323C C92976 M 102 APPEARANCE HEAD TILT LEFT 712 EXCRETION FEW FECES 712 EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYE-LEFT 680, 687 712 923 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 379 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92976 M 102 EYES RED DISCHARGE EYE-RIGHT 694, 701, 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 WEEK 88 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 712C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C C92977 D 98 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 673, 680, 686 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 924 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 380 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92977 D 98 NORMAL NO REMARKABLE OBSERVATIONS 659, 666 C92978 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92979 M 89 BEHAVIOR COLD TO TOUCH ENTIRE BODY 622 DISCHARGE GENITAL RED IN COLOR 622 EXCRETION NO FECES 617, 622 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 554, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 622C 925 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 381 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92980 M 63 APPEARANCE MALOCCLUSION 204, 211, 218, 260, 281, 288, 316, 337, 344, 351, 358, 365, 400 407, 414, 421, 428, 435 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 225, 232, 239, 246, 253 MASS(ES) AXILLARY REGION-LEFT WEEK 38 LIMB-HIND-RIGHT WEEK 55 260, 267, 274, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 414C, 421C, 428C, 435C 379, 386, 393C, 400C, 407C, 414C, 421C, 428C, 435C C92981 M 72 APPEARANCE HEAD TILT LEFT 502 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 502 EXCRETION NO FECES 502 926 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 382 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92981 M 72 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 477, 484, 502 ROUGH HAIR COAT 498, 502 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 491 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C92982 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92983 T 105 EXCRETION FEW FECES 729, 731 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 722 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645 927 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 383 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92983 T 105 MASS(ES) INGUINAL REGION-LEFT WEEK 94 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 731C VENTRAL-CERVICAL WEEK 96 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 C92984 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 463, 470, 477, 484, 491, 498, 505, 512 MASS(ES) AXILLARY REGION-LEFT WEEK 75 WEEK 93 INGUINAL REGION-RIGHT WEEK 75 VENTRAL-ABDOMINAL WEEK 60 WEEK 104 VENTRAL-ABDOMINAL-LEFT WEEK 75 519, 526 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 519, 526, 533, 540, 547, 554, 561, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C 414C, 421C, 428C, 435C, 442C, 449C, 456C 722C, 729C, 732C 519, 526 928 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 384 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92984 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 519, 526, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C C92985 M 98 APPEARANCE MALOCCLUSION MISSING TEETH 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680 204, 211, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 323, 330, 337, 344 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 INGUINAL REGION-RIGHT WEEK 96 VENTRAL-ABDOMINAL-LEFT WEEK 82 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C 666C, 673C, 680C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C 929 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 385 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92985 M 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 95 659C, 666C, 673C, 680C VENTRAL-THORACIC-LEFT WEEK 95 659C, 666C, 673C, 680C C92986 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 519, 526, 533, 540, 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 561, 568, 575, 582 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 85 INGUINAL REGION-RIGHT WEEK 76 WEEK 100 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 526C, 533C, 540C, 547C, 554C 694C, 701C, 708C, 715C 930 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 386 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92987 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92988 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C92989 M 84 APPEARANCE MALOCCLUSION SWOLLEN MOUTH 64, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 540, 547 554, 561, 568, 575, 582 358, 365 DISCHARGE GENITAL RED IN COLOR 547 NASAL RED IN COLOR 358 EXCRETION NO FECES 582 EYES RED DISCHARGE EYE-RIGHT 519, 526, 533 931 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 387 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F2 DOSE: 0.5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92989 M 84 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 582 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 21-22, 29, 36, 43, 50, 57, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351 C92990 M 94 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 463, 470, 477, 484, 491, 498 MASS(ES) AXILLARY REGION-LEFT WEEK 73 VENTRAL-ABDOMINAL WEEK 63 VENTRAL-CERVICAL-RIGHT WEEK 89 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652-653C 435C, 442C, 449C, 456C 617C, 624C, 631C, 638C, 645C, 652-653C 932 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 388 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92931 F2 477 69 TO 490 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 15 X 15MM C92932 F2 197 29 TO 260 38 MASS(ES) DORSAL WEEK 29 20 X 20MM 267 39 TO 274 40 MASS(ES) DORSAL WEEK 29 35 X 30MM 120 18 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 MASS 30 X 25MM 127 19 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 25 X 20MM 134 20 TO 141 21 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 30 X 15MM 148 22 TO 190 28 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 50 X 15MM 197 29 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 35 X 10MM 933 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 389 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92932 F2 204 30 TO 211 31 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 35 X 15MM 218 32 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 35 X 20MM 225 33 TO 232 34 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 45 X 45MM 239 35 TO 260 38 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 35 X 40MM 267 39 TO 285 41 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 45 X 35MM 169 25 TO 197 29 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 25 X 20MM 204 30 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 30 X 25MM 211 31 TO 218 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 30 X 30MM 934 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 390 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92932 F2 225 33 TO 253 37 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 35 X 30MM 260 38 TO 274 40 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 40 X 35MM 281 41 TO 285 41 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 50 X 50MM 169 25 TO 190 28 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 25 10 X 10MM C92933 F2 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 64 20 X 10MM 449 65 TO 463 67 MASS(ES) AXILLARY REGION-LEFT WEEK 64 20 X 20MM 470 68 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 64 40 X 40MM 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 64 45 X 45MM 935 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 391 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92933 F2 568 82 TO 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 64 55 X 45MM 617 89 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 64 60 X 50MM 631 91 TO 642 92 MASS(ES) AXILLARY REGION-LEFT WEEK 64 50 X 50MM 512 74 TO 519 75 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 15 X 15MM 526 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 20 X 15MM 533 77 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 20 X 20MM 561 81 TO 589 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 40 X 35MM 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 55 X 35MM 936 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 392 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92933 F2 603 87 TO 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 65 X 50MM 617 89 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 75 X 75MM 631 91 TO 642 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 70 X 70MM C92935 F2 561 81 TO 568 82 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 65 X 60MM 575 83 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 30 X 30MM 589 85 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 50 X 50MM 449 65 TO 463 67 MASS(ES) SHOULDER-RIGHT WEEK 65 60 X 60MM C92936 F2 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 63 10 X 15MM 937 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 393 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92936 F2 442 64 TO 456 66 MASS(ES) AXILLARY REGION-LEFT WEEK 63 20 X 20MM 463 67 MASS(ES) AXILLARY REGION-LEFT WEEK 63 25 X 25MM 470 68 TO 484 70 MASS(ES) AXILLARY REGION-LEFT WEEK 63 30 X 40MM 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 63 30 X 20MM 498 72 TO 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 63 40 X 30MM 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 63 30 X 50MM 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 63 40 X 40MM 526 76 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 35MM 938 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 394 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92936 F2 547 79 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 63 40 X 45MM 561 81 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 63 40 X 60MM 575 83 TO 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 63 40 X 50MM 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 63 35 X 50MM 603 87 TO 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 50MM 617 89 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 63 60 X 50MM 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 63 60 X 35MM 645 93 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 63 60 X 40MM 939 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 395 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92936 F2 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 63 40 X 40MM 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 40 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 40MM 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 40MM 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 40MM 708 102 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 63 50 X 40MM C92937 F2 428 62 TO 450 65 MASS(ES) VENTRAL-ABDOMINAL WEEK 61 15 X 15MM 295 43 TO 316 46 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 43 10 X 10MM 940 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 396 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92939 F2 428 62 TO 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 58 15 X 15MM 442 64 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 58 20 X 20MM 484 70 MASS(ES) AXILLARY REGION-LEFT WEEK 58 20 X 35MM 491 71 TO 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 58 30 X 30MM 435 63 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 10 X 10MM 456 66 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 15 X 15MM 470 68 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 30 X 25MM 484 70 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 15 X 15MM 941 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 397 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92939 F2 323 47 TO 372 54 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 20 X 25MM 379 55 TO 393 57 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 30 X 25MM 400 58 TO 407 59 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 30 X 40MM 414 60 TO 421 61 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 35 X 35MM 428 62 TO 463 67 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 40 X 40MM 470 68 TO 477 69 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 60 X 50MM 484 70 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 50 X 50MM 491 71 TO 491 71 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 30 X 60MM 942 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 398 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92940 F2 344 50 MASS(ES) AXILLARY REGION-LEFT WEEK 50 15 X 15MM 351 51 MASS(ES) AXILLARY REGION-LEFT WEEK 50 20 X 20MM 358 52 TO 379 55 MASS(ES) AXILLARY REGION-LEFT WEEK 50 30 X 30MM 386 56 TO 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 50 40 X 40MM 400 58 TO 414 60 MASS(ES) AXILLARY REGION-LEFT WEEK 50 40 X 50MM 421 61 TO 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 50 60 X 50MM 449 65 TO 463 67 MASS(ES) AXILLARY REGION-LEFT WEEK 50 65 X 85MM 470 68 TO 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 50 85 X 85MM 943 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 399 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92940 F2 512 74 TO 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 50 90 X 90MM 526 76 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 50 100 X 90MM 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 50 110 X 90MM 582 84 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 50 100 X 90MM 505 73 TO 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 73 20 X 20MM 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 73 30 X 30MM 526 76 TO 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 73 40 X 30MM 547 79 TO 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 73 40 X 40MM 944 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 400 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92940 F2 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 73 50 X 40MM 575 83 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 73 50 X 60MM 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 73 50 X 70MM 603 87 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 73 60 X 70MM 526 76 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 76 10 X 10MM C92942 F2 71 11 MASS(ES) DORSAL WEEK 11 30 X 30MM 78 12 TO 92 14 MASS(ES) DORSAL WEEK 11 30 X 30MM 99 15 TO 204 30 MASS(ES) DORSAL WEEK 11 40 X 30MM 945 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 401 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92942 F2 211 31 TO 218 32 MASS(ES) DORSAL WEEK 11 30 X 30MM 225 33 TO 253 37 MASS(ES) DORSAL WEEK 11 35 X 35MM 260 38 MASS(ES) DORSAL WEEK 11 40 X 35MM 267 39 TO 274 40 MASS(ES) DORSAL WEEK 11 45 X 45MM 281 41 TO 288 42 MASS(ES) DORSAL WEEK 11 50 X 50MM 295 43 TO 309 45 MASS(ES) DORSAL WEEK 11 60 X 50MM 316 46 MASS(ES) DORSAL WEEK 11 80 X 60MM 323 47 TO 348 50 MASS(ES) DORSAL WEEK 11 85 X 80MM 946 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 402 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92942 F2 348 50 TO 348 50 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 50 35 X 25MM C92943 F2 638 92 TO 659 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 15 X 10MM 666 96 TO 677 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 20 X 20MM C92948 F2 669 96 TO 691 99 MASS(ES) VENTRAL-CERVICAL WEEK 96 10 X 10MM C92949 F2 190 28 TO 197 29 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 3 50 X 35MM 204 30 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 3 60 X 35MM 211 31 TO 218 32 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 3 70 X 50MM 225 33 TO 229 33 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 3 80 X 60MM 947 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 403 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92949 F2 141 21 TO 162 24 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 21 15 X 15MM 169 25 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 21 25 X 25MM 176 26 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 21 35 X 25MM 183 27 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 21 40 X 25MM C92951 F2 442 64 TO 463 67 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 10 X 10MM 470 68 TO 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 15 X 15MM 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 25 X 25MM 491 71 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 20 X 15MM 948 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 404 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92951 F2 519 75 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 20 X 20MM 603 87 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 30 X 30MM 645 93 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 40 X 30MM 666 96 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 50 X 35MM 715 103 TO 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 50 X 50MM 442 64 MASS(ES) INGUINAL REGION-LEFT WEEK 64 25 X 20MM 449 65 TO 456 66 MASS(ES) INGUINAL REGION-LEFT WEEK 64 15 X15MM 463 67 TO 484 70 MASS(ES) INGUINAL REGION-LEFT WEEK 64 10 X 10MM 949 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 405 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92951 F2 491 71 TO 498 72 MASS(ES) INGUINAL REGION-LEFT WEEK 64 20 X 20MM 505 73 TO 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 64 10 X 10 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 64 20 X 20MM 561 81 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 10 X 10MM 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 20 X 15MM 589 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 30 X 30MM 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 25 X 35MM 603 87 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 35 X 35MM 950 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 406 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92951 F2 638 92 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 45 X 40MM 666 96 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 50 X 40MM 687 99 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 69 X 45MM 694 100 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 60 X 45MM C92952 F2 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 63 10 X 10MM 442 64 TO 456 66 MASS(ES) AXILLARY REGION-LEFT WEEK 63 20 X 20MM C92954 F2 526 76 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 76 10 X 10MM 533 77 TO 554 80 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 77 30 X 25MM 951 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 407 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92954 F2 561 81 TO 589 85 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 77 35 X 30MM 596 86 TO 645 93 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 77 35 X 40MM 652 94 TO 704 101 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 77 40 X 50MM C92955 F2 519 75 TO 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 75 25 X 10MM 547 79 MASS(ES) INGUINAL REGION-LEFT WEEK 75 25 X 20MM 554 80 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 75 30 X 25MM 582 84 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 75 45 X 35MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 75 60 X 40MM 952 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 408 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92955 F2 610 88 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 75 40 X 40MM 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 10 X 10MM 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 15 X 15MM 589 85 TO 638 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 20 X 20MM 428 62 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 25 X 25MM 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 10 X 10MM 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 15 X 15MM 610 88 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 25 X 25MM 953 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 409 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92956 F2 624 90 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 90 10 X 10MM 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 82 10 X 10MM 575 83 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 82 15 X 15MM 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 82 25 X 15MM 603 87 TO 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 82 25 X 25MM 638 92 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 82 35 X 35MM 652 94 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 82 40 X 40MM 666 96 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 82 40 X 50MM 954 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 410 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92956 F2 680 98 TO 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 82 50 X 60MM 701 101 TO 709 102 MASS(ES) INGUINAL REGION-LEFT WEEK 82 40 X 50MM C92957 F2 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 10 X 10MM 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 15 X 15MM 449 65 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 20 X 20MM 470 68 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 35 X 30MM 484 70 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 40 X 35MM 512 74 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 60 X 50MM 955 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 411 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92957 F2 561 81 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 80 X 65MM 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 95 X 65MM 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 95 X 85MM 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 95 X 70MM 617 89 TO 733 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 100 X 80MM C92960 F2 498 72 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 72 25 X 25MM 512 74 TO 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 72 40 X 40MM 526 76 TO 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 72 50 X 40MM 956 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 412 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92960 F2 547 79 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 72 50 X 50MM 561 81 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 72 70 X 60MM 582 84 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 72 80 X 60MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 72 120 X 80MM 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 105 X 70MM 617 89 TO 624 90 MASS(ES) INGUINAL REGION-LEFT WEEK 72 110 X 80MM 631 91 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 72 100 X 70MM 666 96 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 72 110 X 70MM 957 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 413 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92960 F2 708 102 TO 715 103 MASS(ES) INGUINAL REGION-LEFT WEEK 72 120 X 70MM 722 104 TO 733 105 MASS(ES) INGUINAL REGION-LEFT WEEK 72 120 X 80MM C92961 F2 526 76 MASS(ES) INGUINAL REGION-LEFT WEEK 76 10 X 10MM 533 77 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 76 20 X 20MM 561 81 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 76 35 X 35MM 589 85 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 76 50 X 40MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 76 80 X 60MM 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 76 70 X 50MM 958 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 414 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92961 F2 617 89 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 76 70 X 70MM 652 94 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 76 90 X 70MM 666 96 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 76 100 X 70MM 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 76 110 X 70MM 687 99 TO 691 99 MASS(ES) INGUINAL REGION-LEFT WEEK 76 120 X 70MM C92963 F2 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 72 10 X 10MM 505 73 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 72 10 X 15MM 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 72 15 X 15MM 959 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 415 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92963 F2 526 76 MASS(ES) AXILLARY REGION-LEFT WEEK 72 30 X 30MM 533 77 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 72 20 X 20MM 547 79 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 72 20 X 25MM 561 81 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 72 30 X 30MM 603 87 TO 656 94 MASS(ES) AXILLARY REGION-LEFT WEEK 72 30 X 30MM 519 75 TO 540 78 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 10 X 10MM 547 79 TO 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 15 X 15MM 589 85 TO 652 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 20 X 20MM 960 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 416 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92963 F2 582 84 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 15 X 15MM 603 87 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 10 X 10MM 652 94 TO 656 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 15 X 15MM 470 68 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 20 X 20MM 498 72 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 30 X 30MM 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 40 X 35MM 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 25 X 30MM 526 76 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 30 X 30MM 961 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 417 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92963 F2 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 30 X 40MM 554 80 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 40 X 40MM 582 84 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 50 X 50MM 603 87 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 65 X 75MM 631 91 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 50 X 50MM 652 94 TO 656 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 60 X 50MM C92964 F2 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 83 10 X 10MM 589 85 TO 603 87 MASS(ES) VENTRAL-CERVICAL WEEK 83 20 X 20MM 962 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 418 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92964 F2 610 88 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 83 10 X 10MM 638 92 TO 701 101 MASS(ES) VENTRAL-CERVICAL WEEK 83 15 X 15MM 708 102 MASS(ES) VENTRAL-CERVICAL WEEK 83 20 X 20MM 715 103 TO 731 105 MASS(ES) VENTRAL-CERVICAL WEEK 83 30 X 30MM C92965 F2 414 60 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 20 X 20MM 449 65 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 30 X 30MM 470 68 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 45 X 45MM 512 74 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 65 X 60MM 963 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 419 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92965 F2 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 45 X 50MM 526 76 TO 573 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 45 X 60MM C92971 F2 463 67 MASS(ES) INGUINAL REGION-LEFT WEEK 67 35 X 35MM 470 68 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 67 50 X 50MM 512 74 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 67 70 X 60MM 561 81 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 67 95 X 80MM 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 67 100 X 90MM 603 87 TO 624 90 MASS(ES) INGUINAL REGION-LEFT WEEK 67 110 X 110MM 964 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 420 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92972 F2 498 72 TO 512 74 MASS(ES) SHOULDER-RIGHT WEEK 72 15 X 15MM 519 75 MASS(ES) SHOULDER-RIGHT WEEK 72 25 X 25MM 526 76 TO 540 78 MASS(ES) SHOULDER-RIGHT WEEK 72 30 X 25MM 547 79 TO 554 80 MASS(ES) SHOULDER-RIGHT WEEK 72 30 X 30MM 561 81 TO 610 88 MASS(ES) SHOULDER-RIGHT WEEK 72 45 X 50MM 617 89 TO 631 91 MASS(ES) SHOULDER-RIGHT WEEK 72 55 X 60MM 638 92 TO 670 96 MASS(ES) SHOULDER-RIGHT WEEK 72 55 X 40MM C92975 F2 617 89 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 10 X 10MM 965 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 421 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92975 F2 666 96 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 30 X 30MM 673 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 40 X 35MM 680 98 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 60 X 40MM 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 60 X 50MM 722 104 TO 733 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 70 X 70MM 323 47 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 47 15 X 10MM C92976 F2 575 83 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 10 X 10MM 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 35 X 25MM 966 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 422 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92976 F2 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 20 X 25MM POSTERIOR 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 35 X 20MM POSTERIOR 652 94 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 40 X 20MM POSTERIOR 673 97 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 40 X 30MM POSTERIOR 708 102 TO 712 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 50 X 50MM TWO MASSES APPEAR TO HAVE GROWN TOGETHER 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 88 20 X 20MM 638 92 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 88 25 X 20MM C92979 F2 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 10 X 10MM 967 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 423 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92979 F2 568 82 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 20 X 20MM 582 84 TO 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 25 X 30MM 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 25 X 35MM 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 30 X 30MM 610 88 TO 622 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 40 X 35MM C92980 F2 281 41 MASS(ES) AXILLARY REGION-LEFT WEEK 38 10 X 10MM 288 42 MASS(ES) AXILLARY REGION-LEFT WEEK 38 15 X 20MM 295 43 TO 316 46 MASS(ES) AXILLARY REGION-LEFT WEEK 38 20 X 20MM 968 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 424 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92980 F2 323 47 TO 344 50 MASS(ES) AXILLARY REGION-LEFT WEEK 38 20 X 25MM 351 51 MASS(ES) AXILLARY REGION-LEFT WEEK 38 20 X 30MM 358 52 MASS(ES) AXILLARY REGION-LEFT WEEK 38 30 X 30MM 365 53 TO 379 55 MASS(ES) AXILLARY REGION-LEFT WEEK 38 35 X 40MM 386 56 TO 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 38 25 X 30MM 400 58 TO 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 38 20 X 25MM 428 62 TO 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 38 30 X 25MM 393 57 TO 407 59 MASS(ES) LIMB-HIND-RIGHT WEEK 55 40 X 40MM 969 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 425 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92980 F2 414 60 TO 421 61 MASS(ES) LIMB-HIND-RIGHT WEEK 55 50 X 45MM 428 62 TO 435 63 MASS(ES) LIMB-HIND-RIGHT WEEK 55 60 X 55MM C92983 F2 652 94 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 94 30 X 20MM 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 94 40 X 30MM 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 94 40 X 45MM 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 94 45 X 30MM 708 102 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 94 45 X 50MM C92984 F2 645 93 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 93 10 X 10MM 970 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 426 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92984 F2 680 98 TO 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 93 20 X 15MM 701 101 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 93 15 X 10MM 568 82 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 10 X 10MM 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 15 X 15MM 589 85 TO 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 20 X 20MM 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 25 X 25MM 617 89 TO 645 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 45 X 25MM 652 94 TO 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 50 X 30MM 971 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 427 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92984 F2 694 100 TO 732 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 50 X 40MM 414 60 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 10 X 10MM 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 104 10 X 10MM 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 104 15 X 15MM 533 77 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 15 X 15MM 582 84 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 20 X 25MM 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 25 X 30MM 617 89 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 45 X 30MM 972 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 428 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92984 F2 687 99 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 50 X 40MM C92985 F2 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 15 X 15MM 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 20 X 20MM 554 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 25 X 25MM 561 81 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 45 X 35MM 589 85 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 50 X 50MM 603 87 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 65 X 65MM 645 93 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 80 X 65MM 973 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 429 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92985 F2 666 96 TO 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 80 X 80MM 666 96 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 96 25 X 25MM 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 10 X 10MM 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 15 X 15MM 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 20 X 20MM 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 25 X 25MM 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 30 X 30MM 631 91 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 20 X 20MM 974 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 430 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92985 F2 659 95 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 95 15 X 15MM 680 98 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 95 20 X 20MM 659 95 TO 680 98 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 95 10 X 10MM C92986 F2 589 85 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 85 40 X 25MM 617 89 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 85 50 X 40MM 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 85 55 X 45MM 652 94 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 85 60 X 50MM 680 98 TO 733 105 MASS(ES) INGUINAL REGION-LEFT WEEK 85 70 X 60MM 975 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 431 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92986 F2 526 76 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 10 X 10MM 694 100 TO 715 103 MASS(ES) INGUINAL REGION-RIGHT WEEK 100 20 X 20MM C92990 F2 505 73 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 73 10 X 10MM 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 73 20 X 10MM 526 76 MASS(ES) AXILLARY REGION-LEFT WEEK 73 20 X 15MM 533 77 TO 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 73 30 X 25MM 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 73 35 X 30MM 561 81 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 73 40 X 40MM 976 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 432 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92990 F2 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 73 50 X 30MM 582 84 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 73 50 X 35MM 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 73 65 X 50MM 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 73 50 X 45MM 617 89 TO 653 94 MASS(ES) AXILLARY REGION-LEFT WEEK 73 65 X 55MM 435 63 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL WEEK 63 10 X 10MM 617 89 TO 624 90 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 89 35 X 35MM 631 91 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 89 35 X 60MM 977 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 433 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92990 F2 638 92 TO 645 93 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 89 50 X 60MM 652 94 TO 653 94 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 89 50 X 90MM 978 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 434 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92991 T 103 APPEARANCE MALOCCLUSION 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 719 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 719 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 337, 344, 351, 358 MASS(ES) AXILLARY REGION-LEFT WEEK 44 AXILLARY REGION-RIGHT WEEK 53 302C, 309C, 316C, 323C, 330C 365, 372, 379, 386, 393, 400, 407 C92992 M 84 EXCRETION FEW FECES 578 EYES SQUINTED PERI-ORBITAL-RIGHT 578, 582-583 979 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 435 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92992 M 84 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL 547, 554, 561, 568, 575, 578 DORSAL-THORACIC-RIGHT 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 MASS(ES) AXILLARY REGION-LEFT WEEK 78 DORSAL WEEK 76 INGUINAL REGION-LEFT WEEK 46 WEEK 81 INGUINAL REGION-RIGHT WEEK 76 VENTRAL-CERVICAL WEEK 79 VENTRAL-THORACIC WEEK 62 540C, 547C, 554C, 561C, 568C, 575C, 578C, 582-583C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 578C, 582-583C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C 561C, 568C, 575C, 578C, 582-583C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 578C, 582-583C 547C, 554C, 561C, 568C, 575C, 578C, 582-583C 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 578C, 582-583C 980 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 436 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92993 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C92994 M 82 APPEARANCE MALOCCLUSION MISSING TEETH THIN 141 134 568, 572 EXCRETION NO FECES 572 EYES DISCOLORED/LIGHT RED EYE-LEFT RED DISCHARGE EYE-LEFT 519, 526, 547, 554, 561, 568, 572 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 572 RESPIRATION AUDIBLE 260 SKIN & PELAGE YELLOW HAIR COAT 572 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 218, 225, 316, 323, 407, 414, 22, 29, 36, 43, 50, 127, 148, 155, 162, 232, 239, 246, 253, 330, 337, 344, 351, 421, 428, 435, 442, 57, 64, 71, 78, 85, 169, 176, 183, 190, 267, 274, 281, 288, 358, 365, 372, 379, 449, 456, 463, 470, 92, 99, 106 197, 204, 211 295, 302, 309 386, 393, 400 477, 484, 491 981 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 437 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C92995 M 9 APPEARANCE MISSING TAIL-DISTAL SWOLLEN TAIL-DISTAL 32, 36, 43, 50, 57, 59 32, 36, 43, 50, 57, 59 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB TAIL 57, 59 29, 32, 36, 43, 50, 57, 59 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92996 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C92997 M 83 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 575 RECUMBENT LATERAL 575 EYES RED DISCHARGE EYES 575 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 519, 526, 533, 540, 547, 554, 561, 568 982 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 438 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92997 M 83 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498 MASS(ES) INGUINAL REGION-LEFT WEEK 73 VENTRAL-ABDOMINAL-LEFT WEEK 50 VENTRAL-ABDOMINAL-RIGHT WEEK 74 VENTRAL-CERVICAL WEEK 76 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 344, 351, 358, 365, 372, 379, 386, 393 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 526C, 533C, 540C, 547C, 554C, 561C, 568C C92998 M 46 APPEARANCE THIN 288, 295, 302, 309, 316 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> MILD 316 295, 302, 309, 316 274 EYES SQUINTED PERI-ORBITAL 316 983 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 439 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92998 M 46 RESPIRATION AUDIBLE 274 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 274, 281, 288 316 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 C92999 D 92 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 643 631 596, 603, 610, 617, 624, 631, 638, 643 EYES CLEAR DISCHARGE EYE-RIGHT PROTRUDING EYES OTHER 547, 554 505, 512, 519 400C, 407C, 414C SKIN & PELAGE RED SKIN EARS 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 984 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 440 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C92999 D 92 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 79 AXILLARY REGION-RIGHT WEEK 56 VENTRAL-ABDOMINAL WEEK 59 VENTRAL-ABDOMINAL-RIGHT WEEK 76 VENTRAL-CERVICAL WEEK 79 547, 554, 561 386, 393, 400, 407, 414, 421 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 643C 526C, 533C, 540C, 547C, 554C, 561C, 568C 547C, 554C, 561C, 568C C93000 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93001 M 84 APPEARANCE HEAD TILT RIGHT 575, 582, 584 BEHAVIOR ATAXIC <UNSPECIFIED> 584 985 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 441 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93001 M 84 EXCRETION FEW FECES 584 EYES RED DISCHARGE EYES 584 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 82 VENTRAL-ABDOMINAL-LEFT WEEK 76 568C, 575C, 582C, 584C 526, 533, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 584C 986 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 442 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93002 M 81 APPEARANCE THIN 561 DISCHARGE NASAL RED IN COLOR 547 EYES RED DISCHARGE EYES 526, 533, 561 RESPIRATION IRREGULAR 561 LABORED 561 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323 MASS(ES) AXILLARY REGION-LEFT WEEK 70 AXILLARY REGION-RIGHT WEEK 58 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C 400, 407, 414, 421 987 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 443 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93002 M 81 MASS(ES) INGUINAL REGION-LEFT WEEK 48 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C INGUINAL REGION-RIGHT WEEK 56 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561C VENTRAL-ABDOMINAL WEEK 54 372C, 379C, 386C, 393C C93003 T 103 APPEARANCE MALOCCLUSION 85, 92, 99, 106, 113, 120, 127, 129, 134, 141, 148, 155, 162 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 719 EXCRETION FEW FECES 129 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT YELLOW HAIR COAT 687, 694, 701, 708, 715, 719 715, 719 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 988 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 444 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93003 T 103 MASS(ES) AXILLARY REGION-LEFT WEEK 56 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 719C C93004 M 75 BEHAVIOR ATAXIC <UNSPECIFIED> 520 HYPOACTIVE <UNSPECIFIED> 520 EXCRETION FEW FECES 507, 512 EYES SQUINTED PERI-ORBITAL-RIGHT 505, 507, 512, 519-520 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 989 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 445 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93005 M 90 APPEARANCE THIN 575, 582, 589, 596, 603, 610, 617, 624, 630 BEHAVIOR ATAXIC <UNSPECIFIED> 630 HYPOACTIVE <UNSPECIFIED> 630 EXCRETION FEW FECES 575, 630 EYES RED DISCHARGE EYE-LEFT EYES 540, 547, 554, 561, 568, 575 630 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE HEAD-CRANIAL 470, 477, 484, 547, 554, 561, 568 393, 400, 407 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 MASS(ES) AXILLARY REGION-LEFT WEEK 51 351, 358, 365, 372, 379, 386, 393, 400C, 407C, 414C, 421C, 428C 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 990 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 446 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93005 M 90 MASS(ES) AXILLARY REGION-LEFT WEEK 51 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 630C INGUINAL REGION-RIGHT WEEK 79 547C, 554C, 561C, 568C C93006 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93007 T 103 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 589, 596, 603, 610, 680, 687, 694, 701, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 617, 624, 631, 638, 708, 715, 719 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 561, 568 645, 652, 659 WART LIKE LESION(S) TAIL WEEK 80 554 C93008 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 991 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 447 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93009 M 92 APPEARANCE MALOCCLUSION 631, 638, 642 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 642 EYES DISCOLORED/LIGHT RED EYE-LEFT 642 SCAB EYE-LEFT 642 SQUINTED PERI-ORBITAL-RIGHT 642 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 91 VENTRAL-ABDOMINAL-RIGHT WEEK 76 WEEK 79 631C, 638C, 642C 526 547, 554, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610, 617 624, 631C, 638C, 642C 992 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 448 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93010 M 91 APPEARANCE HEAD TILT RIGHT 631 THIN 631 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 631 EXCRETION FEW FECES 617, 624 EYES RED DISCHARGE EYES 624, 631 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 993 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 449 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93011 T 103 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 519, 526, 533, 540 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 596, 603, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 610, 617, 624, 631, 701, 708, 715, 719 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 547, 554, 561, 568, 638, 645, 652, 659, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 575, 582, 589 666, 673, 680 C93012 M 81 APPEARANCE THIN 505, 512, 519, 526, 533, 540, 547, 554, 561 EYES CLEAR DISCHARGE EYE-LEFT 519, 526 SKIN & PELAGE BLACK SKIN VENTRAL-THORACIC-LEFT SORE/SCAB DORSAL-CERVICAL-RIGHT HEAD-MAXILLARY-RIGHT VENTRAL-THORACIC-LEFT 547, 554, 561 15 15 547, 554, 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 994 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 450 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93012 M 81 NORMAL NO REMARKABLE OBSERVATIONS 211, 218, 225, 232, 239, 246 MASS(ES) AXILLARY REGION-LEFT WEEK 37 INGUINAL REGION-RIGHT WEEK 61 253C, 330C, 407C, 484C, 561C 260C, 337C, 414C, 491C, 267C, 344C, 421C, 498C, 274C, 351C, 428C, 505C, 281C, 358C, 435C, 512C, 288C, 365C, 442C, 519C, 295C, 372C, 449C, 526C, 302C, 379C, 456C, 533C, 309C, 386C, 463C, 540C, 316C, 393C, 470C, 547C, 323C 400C 477C 554C 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C C93013 M 86 APPEARANCE THIN 589, 596 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 547, 554, 561, 596 547, 554, 561, 596 DISCHARGE NASAL RED IN COLOR 547, 554 EYES RED DISCHARGE EYE-RIGHT EYES 547, 554, 561, 568 575, 596 995 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 451 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93013 M 86 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 582 C93014 M 76 APPEARANCE HEAD TILT LEFT HUNCHED 528, 530 530 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 528, 530 EXCRETION FEW FECES 528 NO FECES 530 EYES RED DISCHARGE EYES 519, 526, 528, 530 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 996 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 452 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93014 M 76 MASS(ES) AXILLARY REGION-LEFT WEEK 73 505C, 512C, 519C, 526C, 528C, 530C INGUINAL REGION-LEFT WEEK 72 498C, 505C, 512C, 519C, 526C, 528C, 530C C93015 M 102 APPEARANCE SWOLLEN VAGINA THIN 666 680, 687 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 712 DISCHARGE GENITAL RED IN COLOR 470 EXCRETION NO FECES 712 EYES RED DISCHARGE EYES 712 SKIN & PELAGE RED HAIR COAT DORSAL-CERVICAL 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 997 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 453 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93015 M 102 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 673C, 680C, 687C, 694C, 701C, 708C, 712C C93016 D 82 APPEARANCE HEAD TILT RIGHT MALOCCLUSION MISSING TEETH THIN 504-505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 571 64, 71, 78, 85, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162 169, 176, 183, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 504-505, 512, 519, 526, 533, 540 547, 554, 561, 568, 571 442, 449, 456, 463, 470, 477, 484, 491, 498, 504-505, 512, 519 526, 533, 540, 547, 554, 561, 568, 571 491, 504-505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 571 BEHAVIOR ATAXIC <UNSPECIFIED> 504-505, 512, 519, 526, 533 EXCRETION FEW FECES 491, 504-505, 512, 519 998 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 454 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93016 D 82 SKIN & PELAGE ROUGH HAIR COAT 498, 504-505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 571 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 92, 190, 197, 204 C93017 M 91 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 624, 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 225C, 302C, 379C, 456C, 533C, 610C, 232C, 309C, 386C, 463C, 540C, 617C, 239C, 316C, 393C, 470C, 547C, 624C, 246C, 323C, 400C, 477C, 554C, 631C 253C, 330C, 407C, 484C, 561C, 260C, 337C, 414C, 491C, 568C, 267C, 344C, 421C, 498C, 575C, 274C, 351C, 428C, 505C, 582C, 281C, 358C, 435C, 512C, 589C, 288C, 365C, 442C, 519C, 596C, 295C 372C 449C 526C 603C C93018 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 999 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 455 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93019 M 45 APPEARANCE HUNCHED 309 EXCRETION NO FECES 309 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 302, 309 309 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 MASS(ES) VENTRAL-ABDOMINAL WEEK 43 295C, 302C C93020 D 66 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 461 1000 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 456 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93021 M 63 APPEARANCE HEAD TILT RIGHT THIN 400, 407, 414, 421, 428, 435 421, 428, 435 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT YELLOW HAIR COAT 421, 428 421, 428, 435 435 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 351C, 358C, 365C, 372C, 379C, 386C, 393C C93022 T 103 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL 519, 526, 533, 540, 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 561, 568, 575, 582, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 589, 596 1001 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 457 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93022 T 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 719C C93023 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93024 M 86 APPEARANCE HUNCHED THIN 598 596, 598 EXCRETION FEW FECES 598 EYES RED DISCHARGE EYE-LEFT EYES 561, 568 589, 596, 598 RESPIRATION IRREGULAR 598 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1002 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 458 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93024 M 86 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 598C C93025 D 101 APPEARANCE MALOCCLUSION 71 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 120, 127, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 134, 141, 148, 155, 225, 232, 239, 246, 316, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701, 57, 64, 78, 85, 92, 162, 169, 176, 183, 253, 260, 267, 274, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 704 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 C93026 M 103 APPEARANCE MALOCCLUSION THIN 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 715 1003 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 459 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93026 M 103 EYES CLEAR DISCHARGE EYES 400, 687 PALE EYES 715 RED DISCHARGE EYES 407 SKIN & PELAGE BLUE SKIN VENTRAL-THORACIC-LEFT SORE/SCAB VENTRAL-THORACIC-LEFT 701, 708, 715 715 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85 MASS(ES) AXILLARY REGION-LEFT WEEK 92 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C C93027 M 91 APPEARANCE THIN 631 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 631 EXCRETION FEW FECES 617, 624 1004 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 460 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93027 M 91 EYES RED DISCHARGE EYES 624, 631 SKIN & PELAGE ROUGH HAIR COAT 631 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 603, 610 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 547C, 554C, 561C C93028 M 92 APPEARANCE THIN 638 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 638 EXCRETION FEW FECES 633 1005 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 461 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93028 M 92 EYES RED DISCHARGE EYE-LEFT 631, 633 EYES 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 VENTRAL-ABDOMINAL-RIGHT WEEK 91 610, 617C, 624C, 631C, 633C, 638C 631C, 633C, 638C C93029 M 72 APPEARANCE HEAD TILT LEFT SWOLLEN PAW-HIND-RIGHT THIN 500 499 498-500 BEHAVIOR HYPOACTIVE MODERATE 499 SEVERE 500 1006 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 462 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93029 M 72 EXCRETION FEW FECES 498-499 NO FECES 500 SKIN & PELAGE ROUGH HAIR COAT 498-500 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) VENTRAL-CERVICAL WEEK 33 WEEK 53 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C, 498-500C 365C, 372C, 379C, 386C, 393C C93030 M 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 670 EXCRETION FEW FECES 670 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 617, 624, 631, 638, 645, 652, 659, 666, 670 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1007 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 463 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93030 M 96 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610 C93031 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93032 M 80 APPEARANCE HUNCHED THIN 554, 556 547, 554, 556 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 547, 554, 556 547, 554, 556 DISCHARGE NASAL RED IN COLOR 547 EXCRETION FEW FECES 526, 533, 540, 556 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 1008 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 464 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93032 M 80 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 74 512C, 519C, 526C C93033 M 66 APPEARANCE HEAD TILT RIGHT HUNCHED MALOCCLUSION THIN 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456 456 358 456 DISCHARGE CLEAR ORAL <UNSPECIFIED> 414 MODERATE 456 EXCRETION FEW FECES 456 RESPIRATION AUDIBLE 414 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 288, 295 1009 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 465 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93033 M 66 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL 379 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 MASS(ES) AXILLARY REGION-LEFT WEEK 45 AXILLARY REGION-RIGHT WEEK 45 INGUINAL REGION-LEFT WEEK 43 VENTRAL-ABDOMINAL-RIGHT WEEK 40 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435C, 442C, 449C, 456C 309, 316, 323 295C, 302C, 309C, 316C, 323C 274, 281, 288, 295, 302, 309, 316, 323C, 330C, 337C, 344C, 351C 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C C93034 D 83 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT 526, 533, 540, 547, 554, 561, 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 323, 330, 435 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 337, 344, 351, 358, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 386, 393, 400, 407, 92, 99, 106 183, 190, 197 274, 281, 288 414, 421, 428 1010 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 466 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93034 D 83 MASS(ES) AXILLARY REGION-LEFT WEEK 72 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C AXILLARY REGION-RIGHT WEEK 73 505, 512, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C INGUINAL REGION-LEFT WEEK 73 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C INGUINAL REGION-RIGHT WEEK 43 295C, 302C, 309C, 316C WEEK 73 505, 512, 519, 526, 533, 540, 547C, 554C, 561, 568, 575 VENTRAL-ABDOMINAL-LEFT WEEK 64 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C VENTRAL-ABDOMINAL-RIGHT WEEK 76 526C, 533C, 540C, 547C, 554C VENTRAL-CERVICAL WEEK 53 365C, 372C, 379C C93035 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93036 D 85 APPEARANCE CONVULSIONS OBES TREMORS ENTIRE BODY 592 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 592 592 BEHAVIOR RECUMBENT DORSAL 592 1011 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 467 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93036 D 85 RESPIRATION AUDIBLE 592 IRREGULAR 592 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 575C, 582C, 589C, 592C C93037 M 66 APPEARANCE HEAD TILT LEFT MALOCCLUSION 459 456, 459 BEHAVIOR CIRCLING LEFT 459 HYPOACTIVE <UNSPECIFIED> 459 EXCRETION FEW FECES 456 NO FECES 459 1012 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 468 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93037 M 66 EYES RED DISCHARGE EYE-LEFT 449 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 435 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-LEFT WEEK 58 VENTRAL-CERVICAL-LEFT WEEK 64 400, 407, 414, 421, 428C 442, 449, 456, 459 C93038 M 71 SKIN & PELAGE SORE/SCAB HEAD-CRANIAL 29, 36, 43, 50, 57, 64, 71, 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351, 358 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 365, 372, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 1013 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 469 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93038 M 71 WART LIKE LESION(S) DORSAL-CERVICAL WEEK 7 43 C93039 T 103 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-RIGHT WEEK 75 INGUINAL REGION-LEFT WEEK 66 519, 526 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 719C C93040 T 103 APPEARANCE THIN 715, 719 BEHAVIOR ATAXIC <UNSPECIFIED> 715, 719 SKIN & PELAGE ROUGH HAIR COAT 701, 708, 715, 719 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1014 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 470 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93040 T 103 NORMAL NO REMARKABLE OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 719C C93041 M 83 APPEARANCE HEAD TILT LEFT 575 BEHAVIOR ATAXIC <UNSPECIFIED> 575 HYPOACTIVE <UNSPECIFIED> 575 EXCRETION FEW FECES 575 SKIN & PELAGE RED HAIR COAT LATERA-RIGHT LATERAL-THORACIC-LEFT 561, 568, 575 561, 568, 575 1015 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 471 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93041 M 83 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498 MASS(ES) AXILLARY REGION-LEFT WEEK 73 AXILLARY REGION-RIGHT WEEK 76 WEEK 82 VENTRAL-ABDOMINAL WEEK 53 505, 512, 519, 526, 533, 540C, 547C, 554C, 561C, 568C, 575C 526, 533, 540, 547 568, 575 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C C93042 M 95 APPEARANCE SWOLLEN PAW-HIND-LEFT 603, 610, 617, 624, 631, 638, 645, 652 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 665 RESPIRATION AUDIBLE 665 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT PAW-HIND-LEFT 610, 617, 624, 631, 638, 645, 652, 659, 665 505, 512, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659 1016 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 472 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93042 M 95 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 386, 393 PAWS-HIND 519, 526, 533, 540, 547, 554, 561, 568 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 400, 407, 491, 498 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 414, 421, 428, 435, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 442, 449, 456, 463, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 470, 477, 484 MASS(ES) AXILLARY REGION-LEFT WEEK 82 DORSAL WEEK 92 INGUINAL REGION-LEFT WEEK 79 INGUINAL REGION-RIGHT WEEK 79 VENTRAL-ABDOMINAL-LEFT WEEK 84 568, 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 665C 638C, 645C, 652C, 659C, 665C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 665C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 665C 582 1017 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 473 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93043 M 97 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT 659, 666, 673 519, 526, 533, 540, 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463 MASS(ES) AXILLARY REGION-LEFT WEEK 95 AXILLARY REGION-RIGHT WEEK 68 VENTRAL-ABDOMINAL WEEK 83 VENTRAL-CERVICAL-LEFT WEEK 73 VENTRAL-CERVICAL-RIGHT WEEK 84 659C, 666C, 673C 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C 1018 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 474 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93044 M 66 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN 442, 449, 456 456 449, 456 BEHAVIOR HYPOACTIVE MODERATE 456 EXCRETION FEW FECES 456 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE 449, 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 428, 435 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-RIGHT WEEK 56 386C, 393C, 400C, 407C, 414C, 421C 1019 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 475 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93045 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93046 M 80 APPEARANCE PROLAPSED VAGINA 554 BEHAVIOR COLD TO TOUCH ENTIRE BODY 554 DISCHARGE GENITAL RED IN COLOR 554 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT 498, 505, 512, 519, 526, 533, 540, 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) INGUINAL REGION-LEFT WEEK 73 INGUINAL REGION-RIGHT WEEK 58 505, 512, 519C, 526C, 533C, 540C, 547C, 554C 400, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 1020 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 476 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93046 M 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 554C VENTRAL-CERVICAL-LEFT WEEK 64 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C C93047 T 103 APPEARANCE OBES 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 719 SKIN & PELAGE BROWN SKIN VENTRAL-ABDOMINAL 701 RED HAIR COAT DORSAL-CERVICAL 547 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1021 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 477 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93048 T 103 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 719 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C93049 M 70 APPEARANCE HEAD TILT LEFT 484 BEHAVIOR ATAXIC MODERATE 484 RECUMBENT LATERAL 484 EXCRETION FEW FECES NO FECES 477, 484 484 EYES RED DISCHARGE EYES 463, 470, 477, 484 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 1022 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 478 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F3 DOSE: 2 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93049 M 70 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 C93050 M 98 APPEARANCE THIN 683 EXCRETION FEW FECES 683 NONFORMED FECES 683 EYES RED DISCHARGE EYES 683 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 VENTRAL-ABDOMINAL-RIGHT WEEK 76 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 683C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 683C 1023 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 479 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92991 F3 302 44 TO 316 46 MASS(ES) AXILLARY REGION-LEFT WEEK 44 20 X 15MM 323 47 MASS(ES) AXILLARY REGION-LEFT WEEK 44 15 X 15MM 330 48 MASS(ES) AXILLARY REGION-LEFT WEEK 44 10 X 10MM C92992 F3 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 78 20 X 20MM 547 79 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 78 30 X 30MM 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 78 30 X 30MM 575 83 TO 583 84 MASS(ES) AXILLARY REGION-LEFT WEEK 78 40 X 40MM 526 76 TO 554 80 MASS(ES) DORSAL WEEK 76 15 X 15MM 1024 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 480 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92992 F3 561 81 TO 583 84 MASS(ES) DORSAL WEEK 76 20 X 20MM 316 46 TO 358 52 MASS(ES) INGUINAL REGION-LEFT WEEK 46 15 X 10MM 365 53 TO 386 56 MASS(ES) INGUINAL REGION-LEFT WEEK 46 30 X 30MM 393 57 MASS(ES) INGUINAL REGION-LEFT WEEK 46 30 X 50MM 400 58 TO 407 59 MASS(ES) INGUINAL REGION-LEFT WEEK 46 50 X 35MM 414 60 TO 421 61 MASS(ES) INGUINAL REGION-LEFT WEEK 46 25 X 20MM 561 81 TO 583 84 MASS(ES) INGUINAL REGION-LEFT WEEK 81 10 X 10MM 526 76 TO 540 78 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 15 X 15MM 1025 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 481 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92992 F3 547 79 TO 568 82 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 30 X 20MM 575 83 TO 583 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 40 X 40MM 547 79 TO 578 83 MASS(ES) VENTRAL-CERVICAL WEEK 79 15 X 15MM 582 84 TO 583 84 MASS(ES) VENTRAL-CERVICAL WEEK 79 10 X 10MM 428 62 TO 442 64 MASS(ES) VENTRAL-THORACIC WEEK 62 10 X 10MM 449 65 TO 470 68 MASS(ES) VENTRAL-THORACIC WEEK 62 15 X 15MM 477 69 TO 505 73 MASS(ES) VENTRAL-THORACIC WEEK 62 30 X 20MM 512 74 MASS(ES) VENTRAL-THORACIC WEEK 62 40 X 30MM 1026 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 482 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92992 F3 519 75 MASS(ES) VENTRAL-THORACIC WEEK 62 40 X 20MM 526 76 TO 540 78 MASS(ES) VENTRAL-THORACIC WEEK 62 40 X 30MM 547 79 TO 554 80 MASS(ES) VENTRAL-THORACIC WEEK 62 30 X 20MM 561 81 TO 578 83 MASS(ES) VENTRAL-THORACIC WEEK 62 50 X 25MM 582 84 TO 583 84 MASS(ES) VENTRAL-THORACIC WEEK 62 30 X 30MM C92997 F3 505 73 TO 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 73 10 X 10MM 512 74 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 30 X 30MM 526 76 TO 568 82 MASS(ES) VENTRAL-CERVICAL WEEK 76 10 X 10MM 1027 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 483 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92999 F3 400 58 TO 414 60 EYES OTHER SWOLLEN RIGHT EYELID 407 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 15 X 10MM 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 30 X 20MM 428 62 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 50 X 30MM 435 63 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 45 X 20MM 463 67 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 50 X 30MM 484 70 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 65 X 50MM 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 70 X 60MM 1028 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 484 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C92999 F3 561 81 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 70 X 50MM 575 83 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 60 X 50MM 610 88 TO 643 92 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 40 X 40MM 526 76 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 10 X 10MM 547 79 TO 568 82 MASS(ES) VENTRAL-CERVICAL WEEK 79 10 X 10MM C93001 F3 568 82 TO 584 84 MASS(ES) INGUINAL REGION-LEFT WEEK 82 10 X 10MM 540 78 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 10 X 10MM 561 81 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 30 X 20MM 1029 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 485 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93001 F3 582 84 TO 584 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 30 X 30MM C93002 F3 484 70 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 70 10 X 10MM 505 73 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 70 15 X 15MM 519 75 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 70 20 X 20MM 330 48 TO 337 49 MASS(ES) INGUINAL REGION-LEFT WEEK 48 20 X 20MM 344 50 MASS(ES) INGUINAL REGION-LEFT WEEK 48 20 X 30MM 351 51 TO 379 55 MASS(ES) INGUINAL REGION-LEFT WEEK 48 30 X 30MM 386 56 TO 393 57 MASS(ES) INGUINAL REGION-RIGHT WEEK 56 30 X 40MM 1030 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 486 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93002 F3 400 58 TO 477 69 MASS(ES) INGUINAL REGION-RIGHT WEEK 56 50 X 40MM 484 70 TO 498 72 MASS(ES) INGUINAL REGION-RIGHT WEEK 56 60 X 40MM 505 73 TO 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 56 50 X 45MM 519 75 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 56 50 X 50MM 561 81 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 56 70 X 55MM 372 54 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL WEEK 54 10 X 10MM C93003 F3 386 56 TO 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 56 10 X 10MM 400 58 TO 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 56 20 X 15MM 1031 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 487 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93003 F3 449 65 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 56 30 X 30MM 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 56 35 X 40MM 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 56 40 X 40MM 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 56 40 X 50MM 526 76 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 56 50 X 55MM 547 79 TO 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 56 55 X 70MM 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 56 70 X 70MM 603 87 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 56 80 X 70MM 1032 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 488 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93003 F3 645 93 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 56 90 X 80MM 666 96 TO 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 56 100 X 80MM 701 101 TO 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 56 80 X 80MM 715 103 TO 719 103 MASS(ES) AXILLARY REGION-LEFT WEEK 56 90 X 90MM C93005 F3 400 58 TO 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 51 20 X 15MM 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 51 20 X 20MM 435 63 TO 456 66 MASS(ES) AXILLARY REGION-LEFT WEEK 51 25 X 25M 463 67 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 51 30 X 30MM 1033 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 489 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93005 F3 484 70 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 51 30 X 40MM 505 73 TO 630 90 MASS(ES) AXILLARY REGION-LEFT WEEK 51 40 X 45MM 547 79 TO 568 82 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 10 X 10MM C93009 F3 631 91 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 91 20 X 20MM 638 92 TO 642 92 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 91 25 X 20MM 561 81 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 79 15 X 15MM 575 83 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 79 10 X 10MM 631 91 TO 642 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 79 10 X 10MM 1034 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 490 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93012 F3 253 37 TO 281 41 MASS(ES) AXILLARY REGION-LEFT WEEK 37 25 X 20 MM 288 42 TO 295 43 MASS(ES) AXILLARY REGION-LEFT WEEK 37 30 X 30MM 302 44 TO 386 56 MASS(ES) AXILLARY REGION-LEFT WEEK 37 40 X 40MM 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 37 50 X 50MM 400 58 TO 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 37 60 X 60MM 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 37 90 X 70MM 435 63 TO 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 37 60 X 70MM 449 65 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 37 80 X 70MM 1035 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 491 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93012 F3 484 70 MASS(ES) AXILLARY REGION-LEFT WEEK 37 100 X 100MM 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 37 100 X 80MM 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 37 90 X 80MM 505 73 TO 526 76 MASS(ES) AXILLARY REGION-LEFT WEEK 37 100 X 100MM 533 77 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 37 115 X 95MM 421 61 MASS(ES) INGUINAL REGION-RIGHT WEEK 61 20 X 20MM 428 62 MASS(ES) INGUINAL REGION-RIGHT WEEK 61 40 X 30MM 435 63 TO 477 69 MASS(ES) INGUINAL REGION-RIGHT WEEK 61 20 X 20MM 1036 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 492 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93014 F3 505 73 TO 530 76 MASS(ES) AXILLARY REGION-LEFT WEEK 73 10 X 10MM 498 72 TO 530 76 MASS(ES) INGUINAL REGION-LEFT WEEK 72 10 X 10MM C93015 F3 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 25 X 15MM 687 99 TO 712 102 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 40 X 30MM C93017 F3 225 33 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 10 X 10MM 400 58 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 15 X 15MM 456 66 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 15 X 25MM 484 70 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 35 X 30MM 1037 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 493 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93017 F3 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 30 X 30MM 505 73 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 30 X 40MM 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 40 X 50MM 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 50 X 60MM 617 89 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 60 X 60MM C93019 F3 295 43 TO 302 44 MASS(ES) VENTRAL-ABDOMINAL WEEK 43 15 X 15MM C93021 F3 351 51 TO 393 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 10 X 10MM C93022 F3 603 87 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 25 X 20MM 1038 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 494 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93022 F3 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 30 X 25MM 666 96 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 40 X 30MM 708 102 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 50 X 40MM 715 103 TO 719 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 60 X 50MM C93024 F3 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 15 X 15MM 428 62 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 20 X 20MM 442 64 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 30 X 35MM 463 67 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 40 X 50MM 1039 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 495 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93024 F3 484 70 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 50 X 70MM 519 75 TO 598 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 60 X 70MM C93026 F3 638 92 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 92 20 X 15MM 694 100 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 92 30 X 30MM 708 102 TO 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 92 40 X 30MM C93027 F3 547 79 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 10 X 10MM C93028 F3 617 89 TO 638 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 10 X10MM 631 91 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 15 X 15MM 1040 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 496 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93029 F3 400 58 TO 421 61 MASS(ES) VENTRAL-CERVICAL WEEK 33 30 X 25MM 428 62 TO 463 67 MASS(ES) VENTRAL-CERVICAL WEEK 33 35 X 35MM 470 68 TO 477 69 MASS(ES) VENTRAL-CERVICAL WEEK 33 40 X 35MM 484 70 TO 499 72 MASS(ES) VENTRAL-CERVICAL WEEK 33 40 X 45MM 500 72 TO 500 72 MASS(ES) VENTRAL-CERVICAL WEEK 33 40 X 20MM 365 53 MASS(ES) VENTRAL-CERVICAL WEEK 53 10 X 10MM 372 54 TO 393 57 MASS(ES) VENTRAL-CERVICAL WEEK 53 20 X 20MM C93032 F3 512 74 TO 526 76 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 74 GENITAL 1041 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 497 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93033 F3 435 63 TO 456 66 MASS(ES) AXILLARY REGION-LEFT WEEK 45 10 X 10MM 295 43 MASS(ES) INGUINAL REGION-LEFT WEEK 43 20 X 20MM 302 44 TO 323 47 MASS(ES) INGUINAL REGION-LEFT WEEK 43 25 X 25MM 323 47 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 20 X 15MM 330 48 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 10 X 10MM C93034 F3 498 72 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 72 10 X 10MM 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 72 15 X 15MM 526 76 TO 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 72 20 X 20MM 1042 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 498 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93034 F3 554 80 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 72 15 X 15MM 519 75 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 10 X 10MM 505 73 TO 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 73 20 X 20MM 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 73 50 X 20MM 526 76 MASS(ES) INGUINAL REGION-LEFT WEEK 73 60 X 35MM 533 77 TO 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 73 60 X 50MM 547 79 MASS(ES) INGUINAL REGION-LEFT WEEK 73 80 X 65MM 554 80 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 73 80 X 70MM 1043 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 499 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93034 F3 295 43 TO 316 46 MASS(ES) INGUINAL REGION-RIGHT WEEK 43 15 X 10MM 547 79 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 73 10 X 10MM 442 64 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 10 X 10MM 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 15 X 15MM 505 73 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 20 X 20MM 533 77 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 25 X 25MM 526 76 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 15 X 15MM 365 53 TO 379 55 MASS(ES) VENTRAL-CERVICAL WEEK 53 10 X 10MM 1044 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 500 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93036 F3 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 15 X 15MM 589 85 TO 592 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 20 X 20MM C93037 F3 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 58 15 X 15MM C93038 F3 379 55 TO 386 56 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 20 X 20MM 393 57 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 30 X 25MM 400 58 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 45 X 40MM 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 45 X 40MM 414 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 45 X 40MM 1045 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 501 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93038 F3 421 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 45 X 40MM 428 62 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 70 X 60MM 435 63 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 70 X 60MM 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 70 X 60MM 449 65 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 70 X 60MM 456 66 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 90 X 70MM 463 67 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 90 X 70MM 470 68 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 90 X 80MM 1046 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 502 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93038 F3 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 90 X 80MM 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 115 X 115MM 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 115 X 115MM 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 115 X 115MM 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 115 X 115MM C93039 F3 456 66 TO 484 70 MASS(ES) INGUINAL REGION-LEFT WEEK 66 20 X 15MM 491 71 TO 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 66 30 X 20MM 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 66 30 X 30MM 1047 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 503 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93039 F3 526 76 TO 533 77 MASS(ES) INGUINAL REGION-LEFT WEEK 66 30 X 35MM 540 78 TO 547 79 MASS(ES) INGUINAL REGION-LEFT WEEK 66 40 X 40MM 554 80 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 66 50 X 50MM 603 87 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 66 55 X 55MM 645 93 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 66 60 X 55MM 666 96 TO 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 66 60 X 60MM 715 103 TO 719 103 MASS(ES) INGUINAL REGION-LEFT WEEK 66 70 X 60MM C93040 F3 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 10 X 10MM 1048 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 504 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93040 F3 547 79 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 15 X 10MM 610 88 TO 617 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 15 X 15MM 624 90 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 10 X 10MM 645 93 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 15 X 10MM 659 95 TO 719 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 20 X 15MM C93041 F3 540 78 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 73 10 X 10MM 365 53 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 53 15 X 10MM C93042 F3 582 84 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 82 10 X 10MM 1049 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 505 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93042 F3 610 88 TO 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 82 15 X 15MM 652 94 TO 665 95 MASS(ES) AXILLARY REGION-LEFT WEEK 82 10 X 10MM 638 92 TO 652 94 MASS(ES) DORSAL WEEK 92 10 X 10MM 659 95 TO 665 95 MASS(ES) DORSAL WEEK 92 30 X 30MM 547 79 TO 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 79 10 X 10MM 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 79 20 X 20MM 575 83 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 79 30 X 30MM 589 85 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 79 40 X 30MM 1050 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 506 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93042 F3 610 88 TO 624 90 MASS(ES) INGUINAL REGION-LEFT WEEK 79 40 X 40MM 631 91 TO 665 95 MASS(ES) INGUINAL REGION-LEFT WEEK 79 70 X 50MM 547 79 TO 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 10 X 10MM 603 87 TO 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 15 X 15MM 617 89 TO 665 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 10 X 10MM C93043 F3 659 95 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 95 15 X 15MM 470 68 TO 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 20 X 15MM 484 70 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 30 X 30MM 1051 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 507 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93043 F3 505 73 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 50 X 50MM 547 79 TO 554 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 70 X 60MM 561 81 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 80 X 60MM 603 87 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 90 X 60MM 645 93 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 100 X 70MM 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 20 X 10MM 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 20 X 20MM 603 87 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 25 X 25MM 1052 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 508 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93043 F3 631 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 30 X 30MM 638 92 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 40 X 35MM 505 73 TO 519 75 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 10 X 10MM 526 76 TO 533 77 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 15 X 15MM 540 78 TO 596 86 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 20 X 20MM 603 87 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 30 X 30MM 610 88 TO 666 96 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 20 X 20MM 673 97 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 40 X 50MM 1053 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 509 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93043 F3 673 97 TO 673 97 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 20 X 20MM 582 84 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 84 20 X 20MM 589 85 TO 624 90 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 84 30 X 30MM 631 91 TO 645 93 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 84 50 X 45MM 652 94 TO 673 97 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 84 50 X 55MM C93044 F3 386 56 TO 421 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 56 10 X 10MM C93046 F3 519 75 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 73 10 X 10MM 407 59 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 10 X 10MM 1054 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 510 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93046 F3 414 60 TO 421 61 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 20 X 20MM 428 62 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 50 X 50MM 435 63 TO 477 69 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 40 X 35MM 484 70 TO 519 75 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 60 X 70MM 526 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 70 X 70MM 533 77 TO 547 79 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 80 X 80MM 554 80 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 90 X 90MM 442 64 TO 463 67 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 64 10 X 10MM 1055 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 511 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93046 F3 470 68 TO 477 69 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 64 20 X 20MM 484 70 TO 491 71 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 64 30 X 30MM 498 72 TO 512 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 64 20 X 20MM 519 75 TO 554 80 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 64 30 X 25MM C93050 F3 547 79 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 20 X 15MM 561 81 TO 568 82 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 40 X 40MM 575 83 TO 589 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 30 X 30MM 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 45 X 35MM 1056 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 512 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93050 F3 603 87 TO 638 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 50 X 40MM 645 93 TO 659 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 65 X 50MM 666 96 TO 683 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 65 X 60MM 526 76 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 15 X 10MM 554 80 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 30 X 30MM 575 83 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 40 X 30MM 610 88 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 40 X 35MM 631 91 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 50 X 35MM 1057 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 513 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93050 F3 652 94 TO 683 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 50 X 50MM 1058 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 514 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93051 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93052 M 72 APPEARANCE HEAD TILT LEFT 489, 491, 498, 500 BEHAVIOR COLD TO TOUCH LIMBS-ALL 500 EYES RED DISCHARGE EYE-LEFT 500 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C93053 M 66 APPEARANCE HEAD TILT RIGHT MALOCCLUSION 459 449, 456, 459 SKIN & PELAGE RED HAIR COAT LATERAL-CERVICAL-RIGHT YELLOW HAIR COAT 449 421, 428, 435, 442, 449 1059 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 515 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93053 M 66 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 449C, 456C, 459C C93054 M 72 APPEARANCE HEAD TILT RIGHT 504 THIN 504 BEHAVIOR ATAXIC <UNSPECIFIED> 504 EXCRETION FEW FECES 504 EYES RED DISCHARGE EYES 504 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1060 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 516 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93054 M 72 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 MASS(ES) INGUINAL REGION-LEFT WEEK 71 491C, 498C, 504C C93055 M 77 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 533 EXCRETION FEW FECES 532-533 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 533 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1061 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 517 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93056 M 99 APPEARANCE THIN 687, 691 EXCRETION FEW FECES 691 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 INGUINAL REGION-RIGHT WEEK 95 VENTRAL-ABDOMINAL WEEK 99 645, 652 659C, 666C, 673C, 680C, 687C, 691C 687C, 691C C93057 M 99 APPEARANCE THIN 691 EXCRETION FEW FECES 691 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 554 1062 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 518 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93057 M 99 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 VENTRAL-ABDOMINAL WEEK 77 WEEK 97 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 691C 533, 540C, 547C 673C, 680C, 687C, 691C C93058 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93059 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 1063 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 519 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93060 T 105 APPEARANCE MALOCCLUSION 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 733 EYES CLEAR DISCHARGE EYE-LEFT 701 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 MASS(ES) AXILLARY REGION-LEFT WEEK 93 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 733 C93061 D 80 APPEARANCE HUNCHED THIN 554, 558 554, 558 EXCRETION FEW FECES 533, 540, 554, 558 SKIN & PELAGE PALE ENTIRE BODY 533, 540, 547, 554, 558 1064 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 520 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93061 D 80 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 519, 526 MASS(ES) AXILLARY REGION-LEFT WEEK 73 505, 512 C93062 T 105 APPEARANCE MALOCCLUSION 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C93063 T 105 EYES RED DISCHARGE EYE-RIGHT 246, 253, 260, 267, 274, 281, 295, 302, 309, 316, 323, 330, 337 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 1065 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 521 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93063 T 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 288, 617, 624, 631, 638, 645 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 INGUINAL REGION-RIGHT WEEK 101 VENTRAL-CERVICAL WEEK 102 694C, 701C, 708C, 715C, 722C, 729C, 731C 701, 708, 715, 722, 729, 731 708C, 715C, 722C, 729C, 731C C93064 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93065 M 93 APPEARANCE HUNCHED THIN 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 638 554, 561, 568, 575, 582, 589, 596, 603, 610 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 533, 540, 547, 554, 561, 568 EXCRETION FEW FECES 532-533 1066 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 522 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93065 M 93 EYES RED DISCHARGE EYE-LEFT 540, 547, 554 EYES 532-533 RESPIRATION AUDIBLE 617, 645 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 87 AXILLARY REGION-RIGHT WEEK 83 VENTRAL-ABDOMINAL-RIGHT WEEK 86 VENTRAL-CERVICAL WEEK 72 603, 610, 617, 624, 631, 638, 645 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 596, 603, 610, 617, 624, 631, 638C, 645C 498C, 505C, 512C, 519C, 526C, 532-533C, 540C, 547C, 554C, 561C 568C 1067 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 523 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93066 M 93 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 596, 603, 610, 617, 624, 631, 638, 645 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 MASS(ES) AXILLARY REGION-LEFT WEEK 84 INGUINAL REGION-LEFT WEEK 70 VENTRAL-ABDOMINAL-LEFT WEEK 54 VENTRAL-ABDOMINAL-RIGHT WEEK 70 582, 589, 596, 603, 610C, 617C, 624C, 631C, 638C, 645C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C 1068 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 524 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93067 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93068 D 103 APPEARANCE HEAD TILT RIGHT MALOCCLUSION THIN 715, 718 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 274, 281, 288, 295, 302, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 718 715, 718 SKIN & PELAGE ALOPECIA 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 718 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 309, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512 C93069 M 101 SKIN & PELAGE ALOPECIA 470, 568, 575, 617, 624, 673, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 477 1069 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 525 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93069 M 101 NORMAL NO REMARKABLE OBSERVATIONS 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 582 589 MASS(ES) AXILLARY REGION-LEFT WEEK 87 AXILLARY REGION-RIGHT WEEK 86 INGUINAL REGION-LEFT WEEK 93 VENTRAL-THORACIC-RIGHT WEEK 92 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701-702C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701-702C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701-702C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701-702C C93070 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93071 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93072 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 1070 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 526 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93072 T 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C INGUINAL REGION-LEFT WEEK 103 715C, 722C, 729C, 732C C93073 D 75 APPEARANCE HEAD TILT RIGHT HUNCHED THIN 505, 512, 519, 522 519, 522 498, 505, 512, 519, 522 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 505, 512 519, 522 EXCRETION FEW FECES 491, 498, 505, 512 RESPIRATION AUDIBLE 519, 522 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1071 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 527 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93074 M 83 APPEARANCE HEAD TILT RIGHT 561, 568, 575 BEHAVIOR ATAXIC <UNSPECIFIED> 575 HYPOACTIVE <UNSPECIFIED> 575 EXCRETION FEW FECES 316, 575 EYES RED DISCHARGE EYE-RIGHT 568 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 554 1072 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 528 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93075 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93076 M 94 APPEARANCE MALOCCLUSION MISSING TEETH THIN 43, 50, 57, 64, 71, 148, 155, 162, 169, 239, 246, 253, 260, 330, 337, 344, 351, 421, 428, 435, 442, 512, 519, 526, 533, 603, 610, 617, 624, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141 176, 183, 190, 197, 204, 211, 218, 225, 232 267, 274, 281, 288, 295, 302, 309, 316, 323 358, 365, 372, 379, 386, 393, 400, 407, 414 449, 456, 463, 470, 477, 484, 491, 498, 505 540, 547, 554, 561, 568, 575, 582, 589, 596 631, 638, 645, 652-653 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652-653 652-653 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 645, 652-653 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 LIMB-HIND-LEFT WEEK 59 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652-653C 407, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 1073 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 529 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93076 M 94 MASS(ES) LIMB-HIND-LEFT WEEK 59 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652-653C C93077 T 105 APPEARANCE SWOLLEN TAIL-PROXIMAL 631, 638, 645, 652, 659, 666 SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL 708, 715, 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 101 TAIL-PROXIMAL WEEK 97 701C, 708C, 715C, 722C, 729C, 733C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 1074 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 530 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93078 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 104 VENTRAL-ABDOMINAL WEEK 98 VENTRAL-ABDOMINAL-LEFT WEEK 94 VENTRAL-ABDOMINAL-RIGHT WEEK 89 722, 729, 731 680C, 687C, 694C, 701C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 731C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 722C, 729C, 731C C93079 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 101 701C, 708C, 715C, 722C, 729C, 732C 1075 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 531 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93079 T 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 732C C93080 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 733 C93081 T 105 APPEARANCE MALOCCLUSION 624, 631, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 687, 694, 701, 708, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 638, 645, 715, 722 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 652, 659, 666 1076 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 532 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93082 D 97 EXCRETION FEW FECES 673, 677 EYES PALE EYES 652, 659, 666, 673, 677 SKIN & PELAGE YELLOW HAIR YELLOW SKIN PAWS-ALL TAIL COAT 673, 677 673, 677 673, 677 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 505, 512, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 414, 421, 428, 435, 519, 526, 533, 540, 610, 617, 624, 631, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 442, 449, 456, 463, 547, 554, 561, 568, 638, 645 92, 99, 106 183, 190, 197 274, 281, 288 484, 491, 498 575, 582, 589 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 LIMB-FRONT-LEFT WEEK 45 470, 477 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407 1077 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 533 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93083 M 105 APPEARANCE THIN 729 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) VENTRAL-ABDOMINAL WEEK 95 VENTRAL-CERVICAL-LEFT WEEK 87 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C C93084 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93085 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1078 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 534 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93086 M 90 APPEARANCE HEAD TILT LEFT SWOLLEN MOUTH NOSE THIN 568, 575, 582, 589, 596, 601, 603, 610, 617, 624, 630 601, 603, 610 601, 603, 610 568, 575, 582, 589, 596, 601, 603, 610, 617, 624, 630 BEHAVIOR ATAXIC <UNSPECIFIED> 630 HYPOACTIVE <UNSPECIFIED> 630 EYES DILATED PUPIL EYES 603, 610, 617, 624, 630 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 601, 603, 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 519 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1079 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 535 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93086 M 90 MASS(ES) INGUINAL REGION-LEFT WEEK 72 498C, 505C, 512C VENTRAL-ABDOMINAL-LEFT WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 601C, 603C, 610C, 617C, 624C, 630C C93087 M 100 BEHAVIOR ATAXIC <UNSPECIFIED> 697 HYPOACTIVE <UNSPECIFIED> 697 DISCHARGE NASAL RED IN COLOR 694, 697 EXCRETION FEW FECES 694, 697 EYES RED DISCHARGE EYES 694, 697 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 1080 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 536 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93087 M 100 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 MASS(ES) INGUINAL REGION-LEFT WEEK 93 INGUINAL REGION-RIGHT WEEK 95 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 697C 659C, 666C, 673C, 680C, 687C, 694C, 697C C93088 D 78 APPEARANCE HEAD TILT LEFT THIN 527, 533, 540, 544 505-507, 512 EXCRETION FEW FECES 506-507, 527 EYES RED DISCHARGE EYE-RIGHT 22, 351, 506-507, 512, 519 HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE 505 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT 505-507, 512, 527, 533, 540, 544 505-507, 512, 519, 526-527, 533, 540, 544 1081 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 537 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93088 D 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 456, 463, 484, 491 MASS(ES) AXILLARY REGION-LEFT WEEK 62 WEEK 68 INGUINAL REGION-LEFT WEEK 69 WEEK 72 428C, 435C, 442C, 449C 470 477 498, 505-507, 512 C93089 M 94 APPEARANCE HEAD TILT LEFT MALOCCLUSION SWOLLEN NOSE 652, 656 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 656 155 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 1082 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 538 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93089 M 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 638C, 645C, 652C, 656C INGUINAL REGION-LEFT WEEK 73 505, 512 VENTRAL-ABDOMINAL-LEFT WEEK 87 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 656C VENTRAL-CERVICAL WEEK 88 610, 617, 624, 631C, 638C, 645C, 652C, 656C C93090 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C93091 M 77 APPEARANCE HUNCHED THIN 512 512, 519, 526, 533-535 EYES RED DISCHARGE EYES 512 SKIN & PELAGE PALE ENTIRE BODY 534-535 1083 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 539 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93091 M 77 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-RIGHT 534-535 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316 MASS(ES) LIMB-HIND-RIGHT WEEK 47 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533-535C C93092 M 77 APPEARANCE HEAD TILT LEFT THIN 519, 526, 533, 535 505, 512, 519, 526, 533, 535 BEHAVIOR ATAXIC <UNSPECIFIED> 535 EXCRETION FEW FECES 505 EYES RED DISCHARGE EYES 533, 535 1084 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 540 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93092 M 77 HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE 505 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-RIGHT WEEK 72 498C, 505C, 512C, 519C, 526C, 533C, 535C C93093 M 96 APPEARANCE MALOCCLUSION THIN 71, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 670 670 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 670 EXCRETION FEW FECES 670 1085 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 541 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93093 M 96 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 78 MASS(ES) AXILLARY REGION-LEFT WEEK 91 AXILLARY REGION-RIGHT WEEK 87 VENTRAL-ABDOMINAL-LEFT WEEK 70 VENTRAL-ABDOMINAL-RIGHT WEEK 89 VENTRAL-CERVICAL-LEFT WEEK 94 VENTRAL-CERVICAL-RIGHT WEEK 94 631C, 638C, 645C, 652C, 659C, 666C, 670C 603C, 610C, 617C, 624C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 670C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 670C 652C, 659C, 666C, 670C 652C, 659C, 666C, 670C C93094 M 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 670 COLD TO TOUCH ENTIRE BODY 670 EYES RED DISCHARGE EYES 670 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-RIGHT 659, 666, 670 1086 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 542 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93094 M 96 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 670C C93095 D 105 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 729 COLD TO TOUCH ENTIRE BODY 729 EYES PALE EYES 719, 722, 729 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB AXILLARY REGION-LEFT LATERAL-ABDOMINAL-LEFT 610, 729 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 719, 722, 729 715, 719 1087 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 543 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93095 D 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533 MASS(ES) AXILLARY REGION-LEFT WEEK 79 HEAD-MAXILLARY-RIGHT WEEK 78 INGUINAL REGION-LEFT WEEK 97 SHOULDER-LEFT WEEK 100 VENTRAL-ABDOMINAL WEEK 97 547, 554, 561, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 715C, 719C, 722C, 729C 540 673C, 680C, 687C, 694C, 701C, 708C, 715C, 719C, 722C, 729C 694C, 701C, 708 673C, 680C, 687C, 694C, 701C, 708C, 715C, 719C, 722C, 729C C93096 D 31 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 217 1088 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 544 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93097 M 98 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 680 DISCHARGE OTHER 680C SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-LEFT SORE/SCAB VENTRAL-THORACIC-LEFT 659, 666 673, 680 673, 680 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 428 MASS(ES) AXILLARY REGION-LEFT WEEK 69 VENTRAL-ABDOMINAL-LEFT WEEK 63 VENTRAL-CERVICAL WEEK 78 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 1089 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 545 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93097 M 98 MASS(ES) VENTRAL-CERVICAL WEEK 78 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C VENTRAL-CERVICAL-LEFT WEEK 54 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C C93098 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596, 680, 687 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 603, 610, 617, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 631, 638, 645, 652, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 659, 666, 673 MASS(ES) INGUINAL REGION-LEFT WEEK 100 INGUINAL REGION-RIGHT WEEK 79 694, 701, 708, 715, 722, 729, 732 547C, 554C, 561C C93099 M 96 APPEARANCE HEAD TILT LEFT 671 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 671 1090 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 546 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93099 M 96 DISCHARGE GENITAL RED IN COLOR 671 EXCRETION NO FECES 671 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 69 WEEK 79 INGUINAL REGION-LEFT WEEK 65 VENTRAL-ABDOMINAL-LEFT WEEK 72 VENTRAL-ABDOMINAL-RIGHT WEEK 72 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 671C 449C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 671C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 1091 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 547 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93099 M 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 575C C93100 D 89 APPEARANCE MALOCCLUSION 428, 435, 442 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 113, 120, 127, 134, 141, 148, 204, 211, 218, 225, 232, 239, 295, 302, 309, 316, 323, 330, 386, 393, 400, 407, 414, 421, 498, 505, 512, 519, 526, 533, 610, 617-618 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 449, 456, 463, 470, 561, 568, 575, 582, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 477, 484, 491 589, 596, 603 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 78 540, 547, 554 C93101 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 1092 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 548 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93102 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 309, 316, 435, 442, 526, 533, 652, 659, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 358, 365, 372, 379, 449, 456, 463, 470, 540, 547, 554, 561, 666, 673, 680, 687, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 386, 393, 400, 407, 477, 484, 491, 498, 568, 575, 617, 624, 694, 701, 708, 715, 92, 99, 106 183, 190, 197 274, 281, 302 414, 421, 428 505, 512, 519 631, 638, 645 722, 729, 733 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 42 WEEK 84 VENTRAL-ABDOMINAL-RIGHT WEEK 47 288, 295 582C, 589C, 596C, 603C, 610C 323C, 330C, 337C, 344C, 351C C93103 M 99 APPEARANCE MALOCCLUSION MISSING TEETH 519, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 691 687, 691 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 691 EXCRETION NO FECES 691 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 691 1093 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 549 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93103 M 99 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT 540 PAW-HIND-LEFT 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 691 PAW-HIND-RIGHT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 691 PAWS-HIND 505, 512, 519 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 400, 484, 491, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 76 VENTRAL-CERVICAL-RIGHT WEEK 57 526, 533 393 C93104 M 84 APPEARANCE HUNCHED THIN 561, 568, 575 533, 540, 547, 554, 561, 568, 575, 582-583 EXCRETION FEW FECES 533, 540 1094 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 550 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93104 M 84 EYES RED DISCHARGE EYE-RIGHT 372, 379, 498, 519, 526 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-THORACIC-LEFT YELLOW HAIR COAT 568, 575, 582 554, 561, 568, 575, 582-583 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 MASS(ES) AXILLARY REGION-LEFT WEEK 56 INGUINAL REGION-LEFT WEEK 67 VENTRAL-ABDOMINAL-LEFT WEEK 58 VENTRAL-CERVICAL-LEFT WEEK 65 386, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561C, 568C, 575C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582-583C 449, 456, 463, 470, 477 1095 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 551 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93105 D 89 APPEARANCE HUNCHED MALOCCLUSION SWOLLEN LIMB-HIND-RIGHT THIN 589, 596, 603 589, 596, 603, 610, 617-618 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617-618 589, 596, 603 RESPIRATION LABORED 610 SKIN & PELAGE SORE/SCAB LIMB-HIND-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT 470, 477, 484 498, 505, 519, 526, 533, 540, 547 449 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 456, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463 C93106 M 95 APPEARANCE MISSING TAIL-DISTAL 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 661 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 661 1096 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 552 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93106 M 95 EXCRETION FEW FECES 659, 661 EYES RED DISCHARGE EYES 659, 661 SKIN & PELAGE SORE/SCAB TAIL-DISTAL 554, 561, 568, 575, 582, 589, 596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 C93107 T 105 APPEARANCE MALOCCLUSION MISSING TEETH 162, 169, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 731 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701, 708, 715, 722, 729, 731 1097 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 553 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93107 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 176, 183, 190 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729C, 731C C93108 D 52 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 364 C93109 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93110 M 90 APPEARANCE THIN 624, 630 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 630 EXCRETION FEW FECES 630 NONFORMED FECES 630 1098 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 554 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F4 DOSE: 5 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93110 M 90 EYES RED DISCHARGE EYE-LEFT 630 EYE-RIGHT 470, 477 SKIN & PELAGE ROUGH HAIR COAT 624, 630 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 316, 323, 407, 414, 512, 519, 603, 610, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 330, 337, 344, 351, 421, 428, 435, 442, 526, 533, 540, 547, 617 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 358, 365, 372, 379, 449, 456, 463, 484, 554, 561, 568, 575, 92, 99, 106 183, 190, 197 274, 302, 309 386, 393, 400 491, 498, 505 582, 589, 596 MASS(ES) AXILLARY REGION-LEFT WEEK 41 AXILLARY REGION-RIGHT WEEK 90 281, 288, 295 624C, 630C 1099 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 555 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93053 F4 288 42 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 20 X 20MM 295 43 TO 302 44 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 30 X 25MM 309 45 TO 337 49 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 30 X 30MM 344 50 TO 351 51 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 40 X 30MM 358 52 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 50 X 40MM 365 53 TO 386 56 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 50 X 50MM 393 57 TO 428 62 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 60 X 50MM 435 63 TO 459 66 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 70 X 55MM 1100 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 556 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93054 F4 491 71 TO 504 72 MASS(ES) INGUINAL REGION-LEFT WEEK 71 30 X 20MM C93056 F4 659 95 TO 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 95 20 X 15MM 673 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 95 30 X 25MM 680 98 TO 691 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 95 25 X 25MM 687 99 TO 691 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 99 20 X 20MM C93057 F4 470 68 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 15 X 15MM 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 20 X 20MM 547 79 TO 554 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 30 X 30MM 1101 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 557 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93057 F4 561 81 TO 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 40 X 40MM 596 86 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 60 X 40MM 638 92 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 75 X 75MM 666 96 TO 691 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 75 X 60MM 540 78 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL WEEK 77 15 X 15MM 673 97 TO 691 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 15 X 15MM C93063 F4 694 100 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 10 X 10MM 701 101 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 20 X 20MM 1102 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 558 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93063 F4 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 20 X 30MM 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 30 X 30MM 729 105 TO 731 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 40 X 30MM 708 102 TO 715 103 MASS(ES) VENTRAL-CERVICAL WEEK 102 20 X 20MM 722 104 TO 731 105 MASS(ES) VENTRAL-CERVICAL WEEK 102 35 X 35MM C93065 F4 582 84 TO 610 88 MASS(ES) AXILLARY REGION-RIGHT WEEK 83 10 X 10MM 617 89 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 83 15 X 15MM 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 86 10 X 10MM 1103 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 559 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93065 F4 498 72 TO 568 82 MASS(ES) VENTRAL-CERVICAL WEEK 72 10 X 10MM C93066 F4 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 84 10 X 10MM 617 89 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 84 20 X 15MM 638 92 TO 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 84 30 X 20MM 484 70 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 70 25 X 25MM 512 74 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 70 40 X 40MM 372 54 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 20 X 10MM 393 57 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 25 X 15MM 1104 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 560 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93066 F4 414 60 TO 428 62 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 40 X 20MM 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 40 X 25MM 442 64 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 50 X 40MM 463 67 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 60 X 45MM 484 70 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 70 X 50MM 533 77 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 80 X 60MM 561 81 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 90 X 55MM 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 75 X 50MM 1105 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 561 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93066 F4 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 90 X 50MM 610 88 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 110 X 60MM MASSES ON LEFT SIDE ARE MEASURED AS ONE MASS. 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 110 X 90MM MASSES ON LEFT SIDE ARE MEASURED AS ONE MASS 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 110 X 110MM MASSES ON LEFT SIDE ARE MEASURED AS ONE MASS 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 10 X 10MM 505 73 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 15 X 15MM 568 82 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 20 X 20MM 582 84 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 30 X 30MM 1106 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 562 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93066 F4 617 89 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 35 X 35MM C93069 F4 603 87 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 87 20 X 20MM 631 91 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 87 30 X 30MM 659 95 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 87 40 X 30MM 687 99 TO 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 87 50 X 40MM 701 101 TO 702 101 MASS(ES) AXILLARY REGION-LEFT WEEK 87 60 X 50MM 596 86 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 10 X 10MM 610 88 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 15 X 15MM 1107 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 563 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93069 F4 617 89 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 20 X 20MM 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 25 X 25MM 645 93 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 30 X 25MM 673 97 TO 702 101 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 45 X 30MM 645 93 TO 666 96 MASS(ES) INGUINAL REGION-LEFT WEEK 93 30 X 30MM 673 97 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 93 40 X 35MM 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 93 50 X 40MM 701 101 TO 702 101 MASS(ES) INGUINAL REGION-LEFT WEEK 93 50 X 50MM 1108 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 564 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93069 F4 638 92 TO 659 95 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 92 25 X 25MM 666 96 TO 694 100 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 92 30 X 30MM 701 101 TO 702 101 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 92 40 X 35MM C93072 F4 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 10 X 10MM 610 88 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 15 X 15MM 617 89 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 25 X 25MM 659 95 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 30 X 30MM 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 35 X 30MM 1109 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 565 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93072 F4 687 99 TO 732 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 45 X 40MM 715 103 TO 732 105 MASS(ES) INGUINAL REGION-LEFT WEEK 103 20 X 20MM C93076 F4 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 30 X 30MM 610 88 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 35 X 40MM 631 91 TO 653 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 50 X 50MM 414 60 TO 428 62 MASS(ES) LIMB-HIND-LEFT WEEK 59 55 X 55MM 435 63 TO 484 70 MASS(ES) LIMB-HIND-LEFT WEEK 59 70 X 65MM 491 71 TO 547 79 MASS(ES) LIMB-HIND-LEFT WEEK 59 90 X 75MM 1110 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 566 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93076 F4 554 80 TO 631 91 MASS(ES) LIMB-HIND-LEFT WEEK 59 110 X 100MM 638 92 TO 653 94 MASS(ES) LIMB-HIND-LEFT WEEK 59 120 X 125MM C93077 F4 701 101 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 101 20 X 20MM 729 105 TO 733 105 MASS(ES) AXILLARY REGION-LEFT WEEK 101 30 X 30MM 673 97 TO 687 99 MASS(ES) TAIL-PROXIMAL WEEK 97 25 X 20MM 694 100 TO 701 101 MASS(ES) TAIL-PROXIMAL WEEK 97 30 X 25MM 708 102 TO 733 105 MASS(ES) TAIL-PROXIMAL WEEK 97 25 X 15MM C93078 F4 680 98 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 98 10 X 10MM 1111 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 567 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93078 F4 652 94 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 10 X 10MM 673 97 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 15 X 15MM 680 98 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 25 X 20MM 694 100 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 30 X 25MM 708 102 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 40 X 40MM 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 20 X 20MM 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 30 X 25MM 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 40 X 40MM 1112 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 568 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93078 F4 659 95 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 50 X 45MM 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 50 X 50MM 687 99 TO 694 100 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 60 X 50MM 701 101 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 70 X 65MM C93079 F4 701 101 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 101 10 X 10MM 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 10 X 10MM 484 70 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 20 X 20MM 505 73 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 30 X 25MM 1113 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 569 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93079 F4 526 76 TO 533 77 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 30 X 30MM 540 78 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 30 X 40MM 554 80 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 40 X 40MM 589 85 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 40 X 55MM 638 92 TO 694 100 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 60 X 70MM 701 101 TO 732 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 70 X 80MM C93083 F4 659 95 TO 729 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 95 15 X 10MM 603 87 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 15 X 15MM 1114 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 570 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93083 F4 610 88 TO 624 90 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 30 X 30MM 631 91 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 50 X 45MM 638 92 TO 652 94 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 60 X 55MM 659 95 TO 673 97 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 70 X 65MM 680 98 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 70 X 75MM 687 99 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 80 X 75MM 694 100 TO 722 104 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 90 X 90MM 729 105 TO 729 105 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 100 X 90MM 1115 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 571 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93086 F4 498 72 TO 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 72 15 X 15MM 526 76 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 30 X 30MM 540 78 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 40 X 30MM 575 83 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 40 X 40MM 596 86 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 50 X 40MM 617 89 TO 630 90 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 60 X 50MM C93087 F4 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 93 20 X 20MM 652 94 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 93 30 X 30MM 1116 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 572 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93087 F4 694 100 TO 697 100 MASS(ES) INGUINAL REGION-LEFT WEEK 93 40 X 30MM 659 95 TO 697 100 MASS(ES) INGUINAL REGION-RIGHT WEEK 95 20 X 20MM C93088 F4 428 62 TO 449 65 MASS(ES) AXILLARY REGION-LEFT WEEK 62 10 X 10MM C93089 F4 638 92 TO 656 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 15 X 15MM 603 87 TO 656 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 87 15 X 15MM 631 91 TO 645 93 MASS(ES) VENTRAL-CERVICAL WEEK 88 20 X 20MM 652 94 TO 656 94 MASS(ES) VENTRAL-CERVICAL WEEK 88 25 X 25MM C93091 F4 323 47 MASS(ES) LIMB-HIND-RIGHT WEEK 47 20 X 15MM 1117 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 573 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93091 F4 330 48 TO 351 51 MASS(ES) LIMB-HIND-RIGHT WEEK 47 25 X 25MM 358 52 MASS(ES) LIMB-HIND-RIGHT WEEK 47 25 X 15MM 365 53 TO 379 55 MASS(ES) LIMB-HIND-RIGHT WEEK 47 25 X 30MM 386 56 TO 414 60 MASS(ES) LIMB-HIND-RIGHT WEEK 47 30 X 30MM 421 61 MASS(ES) LIMB-HIND-RIGHT WEEK 47 40 X 40MM 428 62 MASS(ES) LIMB-HIND-RIGHT WEEK 47 30 X 30MM 435 63 TO 442 64 MASS(ES) LIMB-HIND-RIGHT WEEK 47 30 X 40MM 449 65 MASS(ES) LIMB-HIND-RIGHT WEEK 47 60 X 50MM 1118 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 574 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93091 F4 456 66 TO 477 69 MASS(ES) LIMB-HIND-RIGHT WEEK 47 40 X 40MM 484 70 TO 491 71 MASS(ES) LIMB-HIND-RIGHT WEEK 47 50 X 50MM 498 72 TO 526 76 MASS(ES) LIMB-HIND-RIGHT WEEK 47 70 X 70MM 533 77 TO 535 77 MASS(ES) LIMB-HIND-RIGHT WEEK 47 100 X 100MM C93092 F4 498 72 TO 535 77 MASS(ES) AXILLARY REGION-RIGHT WEEK 72 10 X 10MM C93093 F4 631 91 TO 670 96 MASS(ES) AXILLARY REGION-LEFT WEEK 91 10 X 10MM 603 87 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 10 X 10MM 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 30 X 20MM 1119 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 575 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93093 F4 505 73 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 40 X 30MM 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 40 X 40MM 533 77 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 60 X 60MM 582 84 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 65 X 60MM 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 70 X 70MM 617 89 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 75 X 75MM 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 80 X 80MM 659 95 TO 670 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 70 X 70MM 1120 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 576 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93093 F4 617 89 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 25 X 25MM 638 92 TO 670 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 45 X 45MM 652 94 TO 670 96 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 94 15 X 15MM 652 94 TO 670 96 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 94 10 X 10MM C93094 F4 526 76 TO 547 79 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 30 X 30MM 554 80 TO 561 81 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 20 X 20MM 568 82 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 10 X 10MM 575 83 TO 582 84 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 20 X 20MM 1121 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 577 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93094 F4 589 85 TO 596 86 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 30 X 30MM 603 87 TO 624 90 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 40 X 35MM 631 91 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 50 X 35MM 638 92 TO 645 93 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 55 X 45MM 652 94 TO 670 96 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 55 X 60MM C93095 F4 568 82 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 79 10 X 10MM 582 84 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 79 15 X 15MM 603 87 TO 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 79 25 X 25MM 1122 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 578 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93095 F4 652 94 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 79 20 X 20MM 666 96 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 79 20 X 30MM 694 100 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 79 30 X 25MM 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 79 35 X 30MM 715 103 TO 729 105 MASS(ES) AXILLARY REGION-LEFT WEEK 79 40 X 35MM 673 97 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 97 20 X 20MM 694 100 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 97 30 X 30MM 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 97 30 X 45MM 1123 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 579 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93095 F4 715 103 TO 719 103 MASS(ES) INGUINAL REGION-LEFT WEEK 97 40 X 50MM 722 104 TO 729 105 MASS(ES) INGUINAL REGION-LEFT WEEK 97 55 X 40MM 694 100 TO 701 101 MASS(ES) SHOULDER-LEFT WEEK 100 10 X 10MM 673 97 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 10 X 10MM 694 100 TO 729 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 20 X 20MM C93097 F4 680 98 TO 680 98 DISCHARGE OTHER AXILLARY LEFT MASS BLEEDING 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 484 70 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 69 20 X 30MM 1124 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 580 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93097 F4 505 73 TO 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 69 30 X 30MM 526 76 TO 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 69 20 X 20MM 554 80 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 69 30 X 30MM 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 69 40 X 40MM 638 92 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 69 55 X 60MM 435 63 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 15 X 15MM 456 66 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 25 X 20M 505 73 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 40 X 25MM 1125 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 581 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93097 F4 519 75 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 30 X 20MM 540 78 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 30 X 30MM 561 81 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 40 X 30MM 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 40 X 40MM 617 89 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 40 X 50MM 652 94 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 40 X 35MM 666 96 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 40 X 35MM 540 78 MASS(ES) VENTRAL-CERVICAL WEEK 78 15 X 15MM 1126 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 582 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93097 F4 547 79 TO 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 78 20 X 20MM 589 85 MASS(ES) VENTRAL-CERVICAL WEEK 78 30 X 30MM 596 86 TO 610 88 MASS(ES) VENTRAL-CERVICAL WEEK 78 40 X 30MM 617 89 TO 624 90 MASS(ES) VENTRAL-CERVICAL WEEK 78 50 X 40MM 631 91 TO 638 92 MASS(ES) VENTRAL-CERVICAL WEEK 78 40 X 40MM 645 93 TO 680 98 MASS(ES) VENTRAL-CERVICAL WEEK 78 50 X 50MM 372 54 TO 421 61 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 54 15 X 15MM C93098 F4 547 79 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 20 X 20MM 1127 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 583 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93099 F4 477 69 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 561 81 TO 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 69 15 X 15MM 596 86 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 79 20 X 20MM 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 79 30 X 25MM 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 79 20 X 20MM 659 95 TO 671 96 MASS(ES) AXILLARY REGION-LEFT WEEK 79 30 X 20MM 449 65 MASS(ES) INGUINAL REGION-LEFT WEEK 65 10 X 10MM 498 72 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 15 X 15MM 1128 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 584 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93099 F4 561 81 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 20 X 20MM 610 88 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 30 X 25MM 652 94 TO 671 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 20 X 20MM 498 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 20 X 20MM 505 73 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 15 X 15MM C93102 F4 582 84 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 10 X 10MM 323 47 TO 351 51 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 47 10 X 10MM C93104 F4 393 57 TO 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 56 10 X 10MM 1129 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 585 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93104 F4 428 62 TO 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 56 15 X 20MM 442 64 TO 449 65 MASS(ES) AXILLARY REGION-LEFT WEEK 56 20 X 25MM 456 66 TO 526 76 MASS(ES) AXILLARY REGION-LEFT WEEK 56 30 X 35MM 533 77 MASS(ES) AXILLARY REGION-LEFT WEEK 56 40 X 40MM 540 78 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 56 20 X 40MM 561 81 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 56 15 X 35MM 463 67 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 67 15 X 15MM 512 74 TO 526 76 MASS(ES) INGUINAL REGION-LEFT WEEK 67 20 X 20MM 1130 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 586 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93104 F4 400 58 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 15 X 15MM 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 20 X 20MM 428 62 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 30 X 25MM 470 68 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 30 X 30MM 484 70 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 70 X 40MM 491 71 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 40 X 30MM 512 74 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 60 X 40MM 526 76 TO 583 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 45 X 30MM 1131 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 587 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93107 F4 561 81 TO 603 87 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 30 X 30MM 610 88 TO 624 90 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 40 X 30MM 631 91 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 50 X 30MM 638 92 TO 645 93 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 50 X 50MM 652 94 TO 687 99 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 60 X 60MM 694 100 TO 701 101 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 80 X 60MM 708 102 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 70 X 60 MM 715 103 TO 731 105 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 70 X 80MM 1132 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 588 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93110 F4 624 90 TO 630 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 90 20 X 20MM 1133 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 589 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93111 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93112 3 53 EYES RED DISCHARGE EYE-LEFT 316 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY PATTIE MURPHY 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C93113 T 105 EYES SCAB EYE-RIGHT 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 519, 526, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 533, 540, 547, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 1134 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 590 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93113 T 105 NORMAL NO REMARKABLE OBSERVATIONS 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708 MASS(ES) INGUINAL REGION-RIGHT WEEK 73 VENTRAL-ABDOMINAL-LEFT WEEK 103 505, 512 715C, 722C, 729C, 731C C93114 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93115 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 393 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 400, 484, 491, 589, 596, 680, 687, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 519, 526, 533, 603, 610, 617, 624, 694, 701, 708, 715, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 540, 547, 554, 561, 631, 638, 645, 652, 722, 729, 732 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 568, 575, 582 659, 666, 673 MASS(ES) INGUINAL REGION-LEFT WEEK 73 505, 512 1135 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 591 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93116 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C93117 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C93118 M 93 APPEARANCE MALOCCLUSION MISSING TEETH THIN 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 624, 631, 638, 645 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 EYES PROTRUDING EYES 512 SKIN & PELAGE SORE/SCAB MOUTH VENTRAL-ABDOMINAL-LEFT 99 624, 631, 638, 645 1136 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 592 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93118 M 93 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 288, 295, 302 309, 316, 323, 330 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 VENTRAL-ABDOMINAL-RIGHT WEEK 53 337C, 414C, 491C, 568C, 645C 344C, 421C, 498C, 575C, 351C, 428C, 505C, 582C, 358C, 435C, 512C, 589C, 365C, 442C, 519C, 596C, 372C, 449C, 526C, 603C, 379C, 456C, 533C, 610C, 386C, 463C, 540C, 617C, 393C, 470C, 547C, 624C, 400C, 477C, 554C, 631C, 407C 484C 561C 638C 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C C93119 M 92 APPEARANCE MALOCCLUSION 441-442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638 EYES DISCOLORED/LIGHT RED EYE-RIGHT 505, 512 RESPIRATION AUDIBLE 441-442, 449 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB DORSAL MOUTH 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 617, 624, 631, 638 449 1137 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 593 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93119 M 92 SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C93120 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY KATHY EWERS 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 1138 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 594 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93121 M 79 APPEARANCE HEAD TILT LEFT THIN TREMORS ENTIRE BODY 547, 549 505, 512, 519, 526, 533, 540, 547, 549 549 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 549 COLD TO TOUCH ENTIRE BODY 549 EXCRETION FEW FECES 512, 526, 533, 540 SKIN & PELAGE ROUGH HAIR COAT 519, 526, 533, 540, 547, 549 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1139 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 595 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93122 M 92 APPEARANCE THIN 642 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 642 EXCRETION DISCOLORED FECES YELLOW IN COLOR FEW FECES 642 641-642 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 MASS(ES) INGUINAL REGION-LEFT WEEK 71 INGUINAL REGION-RIGHT WEEK 72 WEEK 78 VENTRAL-ABDOMINAL-LEFT WEEK 71 491, 498, 505, 512C 498C, 505C, 575C, 582C, 641-642C 540C, 547C, 617C, 624C, 512C, 589C, 554C, 631C, 519C, 596C, 561C, 638C, 526C, 533C, 603C, 610C, 568C, 575C, 641-642C 540C, 617C, 582C, 547C, 624C, 589C, 554C, 631C, 596C, 561C, 638C 603C, 568C 610C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 641-642C 1140 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 596 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93122 M 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C, 631C, 638C, 641-642C C93123 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93124 T 105 APPEARANCE THIN 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT BLACK SKIN VENTRAL-ABDOMINAL-LEFT PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 729, 732 596, 603, 610, 617, 624, 631, 638 589 666, 673, 680, 687, 694 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701, 708, 715, 722, 729, 732 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1141 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 597 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93124 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 73 505, 512, 519, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C WEEK 94 652C, 659C WEEK 102 708C, 715C, 722C, 729C, 732C DORSAL WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C INGUINAL REGION-LEFT WEEK 76 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C INGUINAL REGION-RIGHT WEEK 72 498C, 505C, 512C SHOULDER-LEFT WEEK 95 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 732C VENTRAL-ABDOMINAL-LEFT WEEK 71 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C VENTRAL-ABDOMINAL-RIGHT WEEK 72 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C WEEK 85 589C WEEK 94 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 732C VENTRAL-CERVICAL-LEFT WEEK 102 708C, 715C, 722C, 729C, 732C VENTRAL-THORACIC-RIGHT WEEK 71 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 1142 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 598 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93124 T 105 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 97 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C C93125 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 C93126 M 58 APPEARANCE THIN 400, 406 BEHAVIOR HYPOACTIVE MODERATE 406 RECUMBENT LATERAL 406 DISCHARGE NASAL RED IN COLOR 406 EYES RED DISCHARGE EYES 406 1143 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 599 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93126 M 58 SKIN & PELAGE ROUGH HAIR COAT 400, 406 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 330, 337, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 47 323 C93127 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93128 M 100 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 694 EYES RED DISCHARGE EYES 694 RESPIRATION LABORED 694 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 512, 519, 526, 533, 540, 547, 554 1144 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 600 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93128 M 100 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 512 C93129 D 100 EYES DILATED PUPIL EYES DISCOLORED/LIGHT RED EYES PROTRUDING EYES 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 666, 673, 680, 687, 694 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694 SKIN & PELAGE SORE/SCAB MOUTH PAW-HIND-RIGHT 554 554, 561, 568 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 1145 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 601 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93129 D 100 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 C93130 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C93131 M 96 APPEARANCE HUNCHED 666 THIN 666 EYES RED DISCHARGE EYES 652, 666 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 652 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 1146 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 602 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93131 M 96 MASS(ES) AXILLARY REGION-LEFT WEEK 91 631, 638, 645, 652 AXILLARY REGION-RIGHT WEEK 68 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C INGUINAL REGION-RIGHT WEEK 89 617C, 624C, 631C, 638C, 645C, 652C, 659C VENTRAL-ABDOMINAL-RIGHT WEEK 58 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C C93132 M 105 APPEARANCE HUNCHED 729 THIN 729 EXCRETION FEW FECES 729 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1147 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 603 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93132 M 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 VENTRAL-ABDOMINAL WEEK 97 VENTRAL-ABDOMINAL-RIGHT WEEK 58 498, 505, 512 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 400C, 407C, 414C, 421C C93133 T 105 APPEARANCE MALOCCLUSION 673 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 680, 687, 694, 701, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 708, 715, 722, 729, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 733 1148 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 604 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93134 D 68 APPEARANCE MALOCCLUSION 78, 85, 92, 99, 106 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 190, 197, 204, 211 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 113, 120, 127, 134 141, 148, 155, 162, 169, 176, 183, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 MASS(ES) SHOULDER-LEFT WEEK 48 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 473C C93135 T 105 APPEARANCE MALOCCLUSION MISSING TEETH THIN 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722, 729, 731 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 731 554, 561, 568, 575 1149 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 605 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93135 T 105 SKIN & PELAGE SORE/SCAB PAWS-HIND 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 MASS(ES) INGUINAL REGION-LEFT WEEK 74 VENTRAL-ABDOMINAL-LEFT WEEK 74 512 519, 526C, 533C C93136 D 55 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 385 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 C93137 T 105 APPEARANCE CONVULSIONS MALOCCLUSION MISSING TEETH 295, 302, 316, 323, 428, 456, 477, 641, 708 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 641, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 708, 722, 729, 732 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE PAW-HIND-LEFT 659, 666, 673, 680, 687, 694, 701, 708 617, 624, 631, 638, 641, 645, 652, 659, 666 1150 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 606 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93137 T 105 SKIN & PELAGE SORE/SCAB PAWS-HIND 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 309, 330, 414, 421, 526 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 337, 344, 351, 358, 435, 442, 449, 463, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 365, 372, 379, 386, 470, 484, 491, 498, 92, 99, 106 183, 190, 197 274, 281, 288 393, 400, 407 505, 512, 519 MASS(ES) AXILLARY REGION-LEFT WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 77 589C, 596C, 603C, 610C, 617C, 624C 533C, 540C, 547C, 554C, 561C, 568C, 575C C93138 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C93139 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575, 582, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 589, 596, 603, 610, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 477 554, 561, 568 645, 652, 659 1151 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 607 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93139 T 105 NORMAL NO REMARKABLE OBSERVATIONS 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 733 MASS(ES) VENTRAL-ABDOMINAL WEEK 68 470C C93140 T 105 EYES RED DISCHARGE EYE-RIGHT 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) AXILLARY REGION-RIGHT WEEK 102 INGUINAL REGION-RIGHT WEEK 104 VENTRAL-ABDOMINAL-RIGHT WEEK 93 708C, 715C, 722C, 729C, 732C 722C, 729C, 732C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 1152 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 608 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93141 M 100 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 698 EXCRETION NO FECES 698 EYES RED DISCHARGE EYE-RIGHT 698 SKIN & PELAGE RED HAIR COAT PERINEAL AREA 694, 698 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 1153 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 609 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93142 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93143 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93144 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93145 D 75 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 519, 523 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-RIGHT WEEK 72 498, 505, 512 C93146 T 105 APPEARANCE THIN 554, 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 582, 589, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 596, 603, 610, 617, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 624, 631, 638, 645, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 568, 575 652, 659, 666 1154 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 610 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93146 T 105 NORMAL NO REMARKABLE OBSERVATIONS 673, 680, 687 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 WEEK 102 VENTRAL-ABDOMINAL-LEFT WEEK 100 694 708C, 715C, 722C, 729C, 732C 694C, 701C, 708C, 715C, 722C, 729C, 732C C93147 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 C93148 D 91 EYES CLEAR DISCHARGE EYE-RIGHT 547, 554, 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 610, 617, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 624, 631, 636 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 589, 596, 603 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 79 547, 554, 561, 568, 575, 582 1155 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 611 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93149 T 105 EXCRETION FEW FECES 701, 708, 715, 722 EYES PROTRUDING EYES 512 SKIN & PELAGE ALOPECIA RED HAIR COAT HEAD-ENTIRE 554 547, 554 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 519, 603, 610, 617, 624 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 561, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 568, 575, 582 MASS(ES) AXILLARY REGION-LEFT WEEK 91 HEAD-MAXILLARY-LEFT WEEK 98 VENTRAL-CERVICAL-LEFT WEEK 100 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 731C 680 694C, 701C, 708C, 715C, 722C, 729C, 731C 1156 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 612 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93150 M 92 APPEARANCE HEAD TILT RIGHT THIN 610, 617, 624, 631, 638, 640 624, 631 BEHAVIOR RECUMBENT LATERAL 640 DISCHARGE NASAL RED IN COLOR 640 EXCRETION FEW FECES 624, 631 EYES SQUINTED PERI-ORBITAL-RIGHT 547, 554 RESPIRATION IRREGULAR 640 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 603 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 582, 589, 596 1157 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 613 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93150 M 92 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 81 561, 568, 575 C93151 T 105 DISCHARGE GENITAL RED IN COLOR 610 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 89 INGUINAL REGION-LEFT WEEK 79 617, 624, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 722C, 729C, 733C 547, 554, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 715C, 722C, 729C, 733C 1158 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 614 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93152 M 66 APPEARANCE HUNCHED THIN 449, 456 456 BEHAVIOR ATAXIC <UNSPECIFIED> 456 DISCHARGE NASAL RED IN COLOR 456 EXCRETION FEW FECES 442, 449 EYES RED DISCHARGE EYES 442, 456 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 449C, 456C 1159 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 615 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93153 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND 505, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 732 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 512, 519, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) DORSAL WEEK 91 VENTRAL-ABDOMINAL-RIGHT WEEK 81 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 732C 561, 568, 575, 582 1160 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 616 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93154 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY JOHN VAN BERKUM 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C93155 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93156 M 86 APPEARANCE THIN 533, 540, 547, 554, 561, 568, 575, 582, 589, 595-596 EXCRETION FEW FECES 531, 533, 540 EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES PALE EYE-RIGHT PROTRUDING EYE-RIGHT 561 568, 575, 582, 589 547, 554 595-596 595-596 1161 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 617 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93156 M 86 EYES RED DISCHARGE EYE-RIGHT 442, 449, 456, 463, 470, 561 EYES 491, 498, 505, 512, 519, 526, 531, 533, 540 SCAB EYE-RIGHT 595-596 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-CERVICAL 435 568, 575, 582, 589, 595-596 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 MASS(ES) VENTRAL-ABDOMINAL WEEK 84 VENTRAL-CERVICAL WEEK 53 582C, 589C, 595-596C 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 531C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589C, 595-596C 1162 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 618 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93157 T 105 EYES PROTRUDING EYES 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 498, 505, 596, 603, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 435, 442, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 449, 456, 463, 470, 547, 554, 561, 568, 638, 645, 652, 659, 729, 733 92, 99, 106 183, 190, 197 274, 281, 288 477, 484, 491 575, 582, 589 666, 673, 680 WART LIKE LESION(S) MOUTH WEEK 47 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428 C93158 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-RIGHT WEEK 71 VENTRAL-ABDOMINAL-RIGHT WEEK 71 491C, 498C, 505C, 512C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 1163 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 619 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93158 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C C93159 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 C93160 M 76 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN 519, 526 519, 526 BEHAVIOR COLD TO TOUCH ENTIRE BODY 526 EXCRETION FEW FECES NO FECES 491, 498, 504-505, 512, 526 506 EYES SQUINTED PERI-ORBITAL-RIGHT 504 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB HEAD-MAXILLARY-RIGHT PAWS-HIND YELLOW HAIR COAT 505-506, 512 197, 204, 211 505-506, 512, 519, 526 519, 526 1164 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 620 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93160 M 76 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 WART LIKE LESION(S) NOSE WEEK 72 498, 504-506, 512, 519, 526 C93161 T 105 APPEARANCE SWOLLEN DORSAL LIMB-HIND-LEFT PAW-HIND-LEFT 589 589, 596, 603, 610 589, 596, 603 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 687, 694, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 617, 624, 631, 701, 708, 715, 722 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 638, 645, 652, 659, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 666, 673, 680 1165 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 621 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93162 T 105 APPEARANCE MALOCCLUSION MISSING TEETH 90, 106, 113, 120, 127, 134, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 90, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 MASS(ES) INGUINAL REGION-LEFT WEEK 102 VENTRAL-ABDOMINAL WEEK 93 708C, 715C, 722C, 729C, 731C 645, 652, 659 1166 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 622 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93163 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY PATTIE MURPHY 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C93164 T 105 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) INGUINAL REGION-LEFT WEEK 77 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 1167 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 623 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93165 T 105 SKIN & PELAGE ALOPECIA SORE/SCAB DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT 554, 561, 666, 673, 680 708, 715, 722, 729, 733 666, 673, 708, 715, 722, 729, 733 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-RIGHT WEEK 78 VENTRAL-THORACIC WEEK 93 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C, 631C, 638C, 645C, 652C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 733C C93166 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 512, 519, 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1168 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 624 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93166 T 105 NORMAL NO REMARKABLE OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 732 C93167 M 39 APPEARANCE MALOCCLUSION MISSING TEETH THIN 253, 260, 267-268 253 268 EXCRETION FEW FECES 260, 267-268 SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT YELLOW HAIR COAT 267-268 260, 267-268 260, 267-268 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246 1169 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 625 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93168 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 732 C93169 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 C93170 M 94 APPEARANCE HEAD TILT RIGHT 645, 652-653 BEHAVIOR ATAXIC <UNSPECIFIED> 653 EYES RED DISCHARGE EYE-LEFT 645, 652-653 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 1170 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 626 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93170 M 94 NORMAL NO REMARKABLE OBSERVATIONS 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 C93171 3 53 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365-366 SURGERY PERFORMED BY JOHN VAN BERKUM 359 ANTIBIOTIC ADMINISTERED 0.5 ML 359-360 C93172 M 78 APPEARANCE HEAD TILT LEFT HUNCHED THIN 519, 526, 528, 533, 540, 546 540, 546 533, 540, 546 BEHAVIOR ATAXIC <UNSPECIFIED> 546 HYPOACTIVE <UNSPECIFIED> 546 EXCRETION FEW FECES 504-505, 512, 528, 533, 540, 546 1171 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 627 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93172 M 78 EYES RED DISCHARGE EYE-RIGHT 546 SQUINTED PERI-ORBITAL-RIGHT 498, 504-505, 512, 519, 526, 528, 533, 540, 546 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-RIGHT 505, 512, 519 512 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C93173 M 102 DISCHARGE NASAL RED IN COLOR 705, 708-709 EXCRETION FEW FECES 705, 708-709 EYES RED DISCHARGE EYES 705, 708-709 1172 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 628 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93173 M 102 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 MASS(ES) AXILLARY REGION-LEFT WEEK 81 SHOULDER-RIGHT WEEK 88 561, 568, 575, 582, 589, 596, 603 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 705C, 708-709C C93174 T 105 APPEARANCE MALOCCLUSION 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) VENTRAL-THORACIC WEEK 100 694C, 701C, 708C, 715C, 722C, 729C, 731C 1173 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 629 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93175 M 92 APPEARANCE THIN TREMORS ENTIRE BODY 638, 641 641 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 641 COLD TO TOUCH ENTIRE BODY 641 EXCRETION FEW FECES 491, 638, 641 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 641C 1174 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 630 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93176 M 88 APPEARANCE HUNCHED THIN 610, 613 610, 613 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL 610, 613 613 EXCRETION NO FECES 613 EYES PROTRUDING EYES RED DISCHARGE EYES 512 610, 613 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT ROUGH HAIR COAT 596, 603, 610, 613 610, 613 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 428, 435, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 442, 449, 456, 463, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 470, 477, 484, 491 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 400 1175 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 631 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93176 M 88 MASS(ES) AXILLARY REGION-LEFT WEEK 59 407, 414, 421 AXILLARY REGION-RIGHT WEEK 55 379, 386, 393 INGUINAL REGION-LEFT WEEK 72 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 613C INGUINAL REGION-RIGHT WEEK 74 512 WEEK 76 526, 533 WEEK 79 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 613C C93177 T 105 APPEARANCE MALOCCLUSION SWOLLEN VENTRAL-ABDOMINAL 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 733 554 DISCHARGE CLEAR ORAL <UNSPECIFIED> 568, 575 EYES PROTRUDING EYES 491, 498, 505, 512 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 708, 715 1176 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 632 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93177 T 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414 C93178 T 105 EYES RED DISCHARGE EYE-LEFT 708, 715, 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 470, 477 554, 561, 568 645, 652, 659 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 67 463 1177 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 633 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93179 T 105 EXCRETION FEW FECES 708 SKIN & PELAGE ALOPECIA SORE/SCAB AXILLARY REGION-LEFT 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652 701, 708, 715, 722, 729, 733 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 MASS(ES) AXILLARY REGION-LEFT WEEK 95 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 733C C93180 M 63 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-RIGHT 57 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 64, 71, 78, 85, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 1178 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 634 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F5 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93180 M 63 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 1179 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 635 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93113 F5 715 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 103 20 X 20MM 722 104 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 103 20 X 20MM C93118 F5 337 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 15MM 344 50 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 20 X 20MM 351 51 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 30 X 25MM 358 52 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 25 X 20 372 54 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 30 X 25MM 393 57 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 30 X 30MM 1180 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 636 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93118 F5 428 62 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 40 X 30MM 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 30 X 30MM 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 40 X 60MM VENTRAL HIND MASSES MERGED TOGETHER. 449 65 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 60 X 70MM VENTRAL HIND MASSES MERGED TOGETHER. 484 70 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 60 X 90MM 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 70 X 90MM 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 70 X 70MM 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 70 X 90MM 1181 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 637 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93118 F5 519 75 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 60 X 75MM 533 77 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 60 X 90MM 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 70 X 100MM 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 70 X 85MM 589 85 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 80 X 85MM 631 91 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 90 X 90MM 365 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 10 X 10MM 372 54 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 15 X 15MM 1182 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 638 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93118 F5 393 57 TO 428 62 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 20 X 20MM 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 25 X 20MM C93122 F5 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 71 10 X 10MM 498 72 TO 533 77 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 10 X 10MM 540 78 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 15 X 15MM 582 84 TO 642 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 20 X 15MM 540 78 TO 568 82 MASS(ES) INGUINAL REGION-RIGHT WEEK 78 10 X 10MM ANTERIOR TO OTHER MASS 575 83 TO 642 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 78 20 X 20MM ANTERIOR TO OTHER MASS 1183 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 639 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93122 F5 519 75 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 10 X 10MM 547 79 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 10MM 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 589 85 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 30MM 617 89 TO 642 92 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 40 X 40MM 463 67 TO 470 68 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 20 X 20MM 477 69 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 20 X 30MM 484 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 30 X 40MM 1184 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 640 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93122 F5 491 71 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 20 X 40MM 519 75 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 20 X 30MM 533 77 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 25 X 35MM 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 35 X 35MM 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 40 X 35MM 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 50 X 40MM 610 88 TO 642 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 60 X 40MM C93124 F5 526 76 TO 617 89 MASS(ES) AXILLARY REGION-LEFT WEEK 73 10 X 10MM 1185 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 641 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93124 F5 652 94 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 94 10 X 10MM 708 102 TO 732 105 MASS(ES) AXILLARY REGION-LEFT WEEK 102 10 X 10MM 526 76 TO 554 80 MASS(ES) DORSAL WEEK 76 10 X 10MM 561 81 TO 582 84 MASS(ES) DORSAL WEEK 76 20 X 20MM 526 76 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 76 10 X 10MM 561 81 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 76 15 X 15MM 498 72 TO 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 10 X 10MM 659 95 TO 732 105 MASS(ES) SHOULDER-LEFT WEEK 95 10 X 10MM 1186 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 642 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93124 F5 491 71 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 10 X 10MM 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 30MM 610 88 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 25MM 659 95 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 20MM 673 97 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 30MM 694 100 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 10 X 10MM 708 102 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 1187 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 643 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93124 F5 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 30 X 40 MM 519 75 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 10 X 10MM 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 35 X 25MM 603 87 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 50 X 30MM 645 93 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 50 X 40MM 659 95 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 50 X 50MM 708 102 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 60 X 60MM 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 70 X 60 MM 1188 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 644 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93124 F5 589 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 10 X 10MM 652 94 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 20 X 15MM 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 10 X 10MM 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 20 X 20MM 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 30 X 30 MM 708 102 TO 732 105 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 102 10 X 10MM 491 71 TO 561 81 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 71 15 X 10MM 568 82 TO 582 84 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 71 20 X 20MM 1189 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 645 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93124 F5 589 85 TO 610 88 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 71 15 X 15MM 617 89 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 71 10 X 10MM 673 97 TO 732 105 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 97 20 X 15MM C93131 F5 470 68 TO 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 20 X 20MM 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 20 X 30MM 498 72 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 20 X 25MM 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 30 X 20MM 526 76 TO 554 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 30 X 30MM 1190 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 646 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93131 F5 561 81 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 50 X 40MM 589 85 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 40 X 40MM 617 89 TO 659 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 15 X 15MM 400 58 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 15 X 10MM 428 62 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 15 X 20MM 435 63 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 20 X 20MM 449 65 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 30 X 30MM 484 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 40 X 40MM 1191 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 647 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93131 F5 491 71 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 30 X 30MM 533 77 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 35 X 40MM 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 40 X 50MM 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 50 X 40MM 589 85 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 50 X 50MM C93132 F5 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 20 X 20MM 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 30 X 30MM 694 100 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 30 X 35MM 1192 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 648 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93132 F5 722 104 TO 729 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 30 X 40MM 400 58 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 10 X 10MM C93134 F5 330 48 TO 337 49 MASS(ES) SHOULDER-LEFT WEEK 48 15 X 20MM 344 50 TO 365 53 MASS(ES) SHOULDER-LEFT WEEK 48 30 X 30MM 372 54 TO 386 56 MASS(ES) SHOULDER-LEFT WEEK 48 40 X 40MM 393 57 MASS(ES) SHOULDER-LEFT WEEK 48 50 X 55MM 400 58 TO 414 60 MASS(ES) SHOULDER-LEFT WEEK 48 60 X 65MM 421 61 MASS(ES) SHOULDER-LEFT WEEK 48 60 X 90MM 1193 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 649 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93134 F5 428 62 MASS(ES) SHOULDER-LEFT WEEK 48 75 X 90MM 435 63 MASS(ES) SHOULDER-LEFT WEEK 48 80 X 85MM 442 64 MASS(ES) SHOULDER-LEFT WEEK 48 80 X 100MM 449 65 TO 473 68 MASS(ES) SHOULDER-LEFT WEEK 48 100 X 100MM C93135 F5 526 76 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 74 10 X 10MM C93137 F5 589 85 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 85 20 X 20MM 533 77 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 77 30 X 30MM C93139 F5 470 68 MASS(ES) VENTRAL-ABDOMINAL WEEK 68 10 X 10MM 1194 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 650 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93140 F5 708 102 TO 732 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 102 10 X 10MM 722 104 TO 732 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 104 10 X 10MM 645 93 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 93 10 X 10MM 666 96 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 93 15 X 15MM 708 102 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 93 15 X 20MM C93146 F5 708 102 TO 732 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 102 10 X 10MM 694 100 TO 722 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 100 15 X 15MM 729 105 TO 732 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 100 20 X 20 MM 1195 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 651 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93149 F5 631 91 TO 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 91 10 X 10MM 652 94 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 91 15 X 15MM 694 100 TO 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 91 20 X 20MM 694 100 TO 708 102 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 100 10 X 10MM 715 103 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 100 15 X 15MM 722 104 TO 731 105 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 100 15 X 15MM C93151 F5 631 91 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 89 10 X 10MM 645 93 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 89 15 X 15MM 1196 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 652 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93151 F5 708 102 TO 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 89 20 X 20MM 729 105 TO 733 105 MASS(ES) AXILLARY REGION-LEFT WEEK 89 30 X 20MM 561 81 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 79 10 X 10MM 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 79 15 X 15MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 79 30 X 30MM 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 79 23 X 45MM 617 89 TO 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 79 35 X 40MM 638 92 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 79 45 X 50MM 1197 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 653 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93151 F5 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 79 45 X 70MM 659 95 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 79 70 X 55MM 680 98 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 79 70 X 80MM 708 102 TO 733 105 MASS(ES) INGUINAL REGION-LEFT WEEK 79 70 X 100MM C93152 F5 365 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 10 X 10MM 372 54 TO 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 20 X 20MM 449 65 TO 456 66 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 25 X 25MM C93153 F5 631 91 TO 645 93 MASS(ES) DORSAL WEEK 91 10 X 10MM 1198 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 654 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93153 F5 652 94 TO 666 96 MASS(ES) DORSAL WEEK 91 15 X 20MM 673 97 TO 680 98 MASS(ES) DORSAL WEEK 91 25 X 20MM 687 99 TO 715 103 MASS(ES) DORSAL WEEK 91 30 X 20MM 722 104 TO 732 105 MASS(ES) DORSAL WEEK 91 40 X 40MM C93156 F5 582 84 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL WEEK 84 10 X 10MM 365 53 TO 372 54 MASS(ES) VENTRAL-CERVICAL WEEK 53 10 X 10MM 379 55 TO 421 61 MASS(ES) VENTRAL-CERVICAL WEEK 53 15 X 20MM 428 62 MASS(ES) VENTRAL-CERVICAL WEEK 53 15 X 35MM 1199 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 655 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93156 F5 435 63 MASS(ES) VENTRAL-CERVICAL WEEK 53 20 X 30MM 442 64 MASS(ES) VENTRAL-CERVICAL WEEK 53 30 X 30MM 449 65 TO 477 69 MASS(ES) VENTRAL-CERVICAL WEEK 53 30 X 40MM 484 70 MASS(ES) VENTRAL-CERVICAL WEEK 53 45 X 55MM 491 71 TO 554 80 MASS(ES) VENTRAL-CERVICAL WEEK 53 40 X 50MM 561 81 MASS(ES) VENTRAL-CERVICAL WEEK 53 50 X 50MM 568 82 TO 595 85 MASS(ES) VENTRAL-CERVICAL WEEK 53 50 X 40MM 596 86 TO 596 86 MASS(ES) VENTRAL-CERVICAL WEEK 53 40 X 50MM 1200 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 656 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93158 F5 491 71 TO 498 72 MASS(ES) INGUINAL REGION-RIGHT WEEK 71 10 X 10MM 505 73 TO 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 71 15 X 15MM 519 75 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 15 X 15MM 533 77 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 20 X 20MM 561 81 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 30 X 20MM 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 60 X 50MM 617 89 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 50 X 40MM 687 99 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 70 X 40MM 1201 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 657 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93158 F5 708 102 TO 731 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 70 X 60MM C93162 F5 708 102 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 102 10 X 10MM C93164 F5 533 77 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 77 10 X 10MM 610 88 TO 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 77 15 X 15MM 638 92 TO 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 77 25 X 20MM 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 77 30 X 20MM 666 96 MASS(ES) INGUINAL REGION-LEFT WEEK 77 40 X 30MM 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 77 30 X 20MM 1202 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 658 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93164 F5 680 98 TO 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 77 40 X 25MM 701 101 TO 715 103 MASS(ES) INGUINAL REGION-LEFT WEEK 77 50 X 30MM 722 104 TO 731 105 MASS(ES) INGUINAL REGION-LEFT WEEK 77 65 X 40MM C93165 F5 540 78 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 78 10 X 10MM 645 93 TO 652 94 MASS(ES) VENTRAL-THORACIC WEEK 93 20 X 20MM 659 95 TO 701 101 MASS(ES) VENTRAL-THORACIC WEEK 93 30 X 20MM 708 102 TO 733 105 MASS(ES) VENTRAL-THORACIC WEEK 93 30 X 30MM C93173 F5 610 88 TO 624 90 MASS(ES) SHOULDER-RIGHT WEEK 88 15 X 25M 1203 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 659 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93173 F5 631 91 MASS(ES) SHOULDER-RIGHT WEEK 88 15 X 20MM 638 92 TO 687 99 MASS(ES) SHOULDER-RIGHT WEEK 88 20 X 25MM 694 100 TO 709 102 MASS(ES) SHOULDER-RIGHT WEEK 88 20 X 30MM C93174 F5 694 100 TO 701 101 MASS(ES) VENTRAL-THORACIC WEEK 100 15 X 15MM 708 102 TO 722 104 MASS(ES) VENTRAL-THORACIC WEEK 100 20 X 20MM 729 105 TO 731 105 MASS(ES) VENTRAL-THORACIC WEEK 100 30 X 20 MM C93175 F5 519 75 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 20 X 20MM 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 30 X 30MM 1204 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 660 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93175 F5 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 40 X 40MM 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 50 X 40MM 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 60 X 60MM 638 92 TO 641 92 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 60 X 50MM C93176 F5 498 72 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 72 10 X 10MM 512 74 MASS(ES) INGUINAL REGION-LEFT WEEK 72 15 X 15MM 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 72 15 X 15MM 526 76 MASS(ES) INGUINAL REGION-LEFT WEEK 72 15 X 15MM 1205 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 661 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93176 F5 533 77 MASS(ES) INGUINAL REGION-LEFT WEEK 72 15 X 15MM 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 72 20 X 20MM 547 79 MASS(ES) INGUINAL REGION-LEFT WEEK 72 20 X 20MM 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 72 20 X 20MM 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 72 20 X 20MM 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 1206 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 662 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93176 F5 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 613 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 613 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 613 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 30 X 20MM 547 79 TO 613 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 10 X 10MM 1207 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 663 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93179 F5 659 95 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 95 15 X 15MM 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 95 20 X 15MM 694 100 TO 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 95 20 X20MM 708 102 TO 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 95 40 X 50MM 722 104 TO 733 105 MASS(ES) AXILLARY REGION-LEFT WEEK 95 40 X 40MM C93180 F5 386 56 TO 407 59 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 30 X 50MM 414 60 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 40 X 60MM 421 61 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 80 X 60MM 1208 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 664 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93180 F5 428 62 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 90 X 80MM 428 62 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 80 X 60MM 435 63 TO 435 63 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 110 X 80MM 1209 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 665 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93181 U 106 APPEARANCE MALOCCLUSION 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 323, 330 337, 344, 351 C93182 U 106 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 393 ROUGH HAIR COAT 393 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 407, 414, 421, 428, 498, 505, 512, 519, 589, 596, 603, 610, 680, 687, 694, 701 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 526, 533, 540, 547, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 463, 470, 477 554, 561, 568 645, 652, 659 MASS(ES) AXILLARY REGION-LEFT WEEK 102 708C, 715C, 722C, 729C, 736C 1210 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 666 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93183 U 106 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) INGUINAL REGION-LEFT WEEK 91 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 736C C93184 U 106 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 561, 568, 708 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 295, 302, 386, 393, 477, 484, 582, 589, 673, 680, 22, 29, 36, 43, 50, 127, 134, 141, 148, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 596, 603, 610, 617, 687, 694, 701 57, 64, 71, 78, 85, 155, 162, 169, 176, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 624, 631, 638, 645, 92, 99, 106 183, 281, 288 365, 372, 379 456, 463, 470 547, 554, 575 652, 659, 666 WART LIKE LESION(S) MOUTH WEEK 102 708, 715, 722, 729, 736 1211 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 667 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93185 U 106 APPEARANCE MALOCCLUSION 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 351 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 736 EYES OPAQUE EYE-LEFT RED DISCHARGE EYE-LEFT 722, 729, 736 288, 295, 302, 309, 316, 323, 330, 337, 344 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 281 C93186 M 75 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302 MASS(ES) VENTRAL-CERVICAL WEEK 45 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519-520C 1212 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 668 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93187 M 104 APPEARANCE HEAD TILT LEFT MALOCCLUSION 725 715, 722, 725 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 722, 725 725 EXCRETION FEW FECES 617, 705, 708, 715, 722, 725 SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT 725 722, 725 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554 MASS(ES) AXILLARY REGION-RIGHT WEEK 102 INGUINAL REGION-LEFT WEEK 81 708C, 715C, 722C, 725C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 1213 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 669 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93187 M 104 MASS(ES) INGUINAL REGION-LEFT WEEK 81 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 705C 708C, 715C, 722C, 725C INGUINAL REGION-RIGHT WEEK 101 701C, 705C C93188 D 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 729, 734 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 70 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729C, 734C 1214 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 670 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93189 M 87 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION MISSING TEETH THIN 605 605 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 605 239, 246, 253, 260 589, 596, 603, 605 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 605 EXCRETION FEW FECES 605 EYES RED DISCHARGE EYE-RIGHT 155 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 162, 169, 176, 183, 190, 197, 204 211, 218, 225 1215 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 671 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93190 U 106 APPEARANCE THIN 701, 708, 715, 722, 729, 736 EXCRETION FEW FECES 680 EYES RED DISCHARGE EYES 701, 708 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT 722, 729, 736 561 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 575, 582, 666, 673, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 589, 596, 603, 610, 687, 694 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 617, 624, 631, 638, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 568 645, 652, 659 1216 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 672 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93191 M 91 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 633 RECUMBENT LATERAL 633 EYES RED DISCHARGE EYES 633 RESPIRATION AUDIBLE 633 LABORED 633 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 C93192 U 106 APPEARANCE MALOCCLUSION 155 SKIN & PELAGE SORE/SCAB MOUTH 64, 71, 78 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 85, 92, 99, 106, 113, 120, 127 1217 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 673 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93192 U 106 NORMAL NO REMARKABLE OBSERVATIONS 134, 141, 148, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 736C C93193 M 76 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 530 EXCRETION FEW FECES 530 NONFORMED FECES 530 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 512, 519, 526, 529-530 512, 519, 526, 529-530 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1218 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 674 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93193 M 76 NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505 C93194 M 92 SKIN & PELAGE ALOPECIA RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT SORE/SCAB DORSAL-CERVICAL LIMBS-HIND VENTRAL-ABDOMINAL-RIGHT VENTRAL-THORACIC 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638 561, 568, 575, 582, 589, 596, 603 582 582, 589, 596, 603, 610, 617, 624, 631, 638 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 MASS(ES) AXILLARY REGION-LEFT WEEK 76 INGUINAL REGION-RIGHT WEEK 81 VENTRAL-ABDOMINAL-LEFT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 81 526, 533, 540 568C, 575C, 582C, 589C, 596C 568, 575, 582, 589, 596, 603C, 610C, 617C, 624C, 631C, 638C 561C 1219 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 675 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93194 M 92 WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 76 526 C93195 D 71 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 C93196 M 88 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 613-614 EXCRETION FEW FECES 613-614 EYES RED DISCHARGE EYES 613-614 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 1220 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 676 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93197 U 106 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 MASS(ES) AXILLARY REGION-LEFT WEEK 99 INGUINAL REGION-LEFT WEEK 93 WEEK 95 687, 694C, 701C, 708C, 715C, 722C, 729C, 736C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 736C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C 736C C93198 U 106 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645 1221 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 677 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93198 U 106 MASS(ES) AXILLARY REGION-RIGHT WEEK 94 652, 659, 666, 673, 680, 687, 694C, 701C, 708C, 715C, 722C, 729C 736C C93199 M 65 APPEARANCE HEAD TILT RIGHT THIN 449-450 442, 449-450 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 C93200 M 96 APPEARANCE THIN 671 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 666, 671 DISCHARGE NASAL RED IN COLOR 666 EXCRETION FEW FECES 659 NO FECES 671 1222 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 678 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93200 M 96 EYES RED DISCHARGE EYE-RIGHT 561, 568, 575, 666 EYES 671 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 666 666, 671 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 589, 596, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 603, 610, 617, 624, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 631, 638, 645, 652 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 582 C93201 U 106 APPEARANCE CONVULSIONS TREMORS ENTIRE BODY 232, 239, 330, 400 687 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 309, 316, 414, 421, 505, 512, 596, 603, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 246, 253, 323, 337, 344, 351, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 57, 64, 71, 78, 85, 155, 162, 169, 176, 260, 267, 274, 281, 358, 365, 372, 379, 456, 463, 470, 477, 547, 554, 561, 568, 638, 645, 680, 694, 92, 99, 106 183, 190, 197 288, 295, 302 386, 393, 407 484, 491, 498 575, 582, 589 701, 708, 715 1223 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 679 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93201 U 106 NORMAL NO REMARKABLE OBSERVATIONS 722, 729, 736 MASS(ES) AXILLARY REGION-RIGHT WEEK 94 652, 659, 666, 673 C93202 U 106 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 736 C93203 M 90 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT SORE/SCAB VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 526, 533 540 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 1224 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 680 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93203 M 90 MASS(ES) AXILLARY REGION-LEFT WEEK 76 526, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 603C, 610C, 617C, 624C WEEK 81 561, 568, 575, 582, 589, 596, 603 AXILLARY REGION-RIGHT WEEK 82 568, 575 INGUINAL REGION-LEFT WEEK 81 561, 568, 575, 582, 589C, 596C, 603C, 610C, 617C, 624C VENTRAL-ABDOMINAL-LEFT WEEK 69 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C VENTRAL-ABDOMINAL-RIGHT WEEK 62 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C VENTRAL-THORACIC-LEFT WEEK 81 561C, 568C, 575C, 582C C93204 D 92 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 642C 1225 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 681 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93205 U 106 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 736 C93206 M 104 APPEARANCE THIN 701, 708, 715, 722, 727 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 727 EXCRETION FEW FECES 673, 701, 708, 715, 722, 727 EYES RED DISCHARGE EYES 708, 722, 727 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 1226 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 682 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93206 M 104 NORMAL NO REMARKABLE OBSERVATIONS 659, 666, 680, 687, 694 C93207 M 41 APPEARANCE HUNCHED THIN 278, 281 278, 281 EXCRETION NO FECES 278 EYES RED DISCHARGE EYES 278, 281 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 64, 71C, 78C, 85C, 92C, 99C, 106C, 113C, 120C, 127C, 134C, 141C 148C, 155C, 162C, 169C, 176C, 183C, 190C, 197C, 204C, 211C, 218C 225C, 232C, 239C, 246C, 253C, 260C, 267C, 274C, 278C, 281C C93208 D 83 BEHAVIOR RECUMBENT STERNAL 576 DISCHARGE GENITAL RED IN COLOR 316 1227 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 683 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93208 D 83 RESPIRATION AUDIBLE 576 IRREGULAR 576 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 575 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 323, 330, 337, 407, 414, 421, 428, 498, 505, 512, 519, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 344, 351, 358, 365, 435, 442, 449, 456, 526, 533, 540, 547, 92, 99, 106 183, 190, 197 274, 281, 288 372, 379, 386 463, 470, 477 554, 561, 568 C93209 U 106 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 736 C93210 M 105 APPEARANCE MALOCCLUSION THIN 141, 148, 414, 421, 428, 435, 463, 470, 477, 582, 589, 596, 603 722, 729, 734 BEHAVIOR HYPOACTIVE <UNSPECIFIED> 729, 734 1228 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 684 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93210 M 105 EXCRETION FEW FECES 705, 708, 722, 729, 734 SKIN & PELAGE YELLOW HAIR COAT 722, 729, 734 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 218, 225, 309, 316, 400, 407, 554, 561, 22, 29, 36, 43, 50, 127, 134, 155, 162, 232, 239, 246, 253, 323, 330, 337, 344, 442, 449, 456, 498, 568, 575 57, 64, 71, 78, 85, 169, 176, 183, 190, 260, 267, 274, 281, 351, 358, 365, 372, 505, 512, 519, 526, 92, 99, 106 197, 204, 211 288, 295, 302 379, 386, 393 533, 540, 547 MASS(ES) INGUINAL REGION-LEFT WEEK 94 INGUINAL REGION-RIGHT WEEK 88 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 705C, 708C, 715C 722C, 729C, 734C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 705C, 708C, 715C, 722C, 729C, 734C C93211 U 106 APPEARANCE CONVULSIONS TREMORS ENTIRE BODY 400, 407, 414, 421, 428, 435, 442, 491, 498, 512, 638, 645, 652 722 680, 687 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 1229 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 685 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93211 U 106 NORMAL NO REMARKABLE OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 449, 456, 463, 470, 477, 484, 505, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 659, 666, 673, 694, 701, 708, 715 MASS(ES) INGUINAL REGION-LEFT WEEK 104 722, 729, 736 C93212 D 84 SKIN & PELAGE SORE/SCAB LIMBS-HIND PAWS-HIND WARM TO TOUCH LIMBS-HIND 533, 540, 547, 554 561, 568, 575, 582, 584 526 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 1230 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 686 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93213 M 84 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN TREMORS ENTIRE BODY 586 586 113, 120, 127, 134, 141, 148, 155, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 586 554, 561, 568, 575, 582, 586 586 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY 561, 568, 575, 582, 586 561, 568, 575, 582, 586 586 DISCHARGE NASAL RED IN COLOR 561 EXCRETION FEW FECES 554, 586 EYES RED DISCHARGE EYE-LEFT 393 1231 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 687 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93213 M 84 SKIN & PELAGE PALE ENTIRE BODY 586 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 162, 169, 176, 183 WART LIKE LESION(S) TAIL-PROXIMAL WEEK 76 526, 533, 540, 547, 554 C93214 D 98 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 684 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 C93215 M 86 APPEARANCE THIN 582, 589, 596 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 596 575, 582, 589, 596 1232 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 688 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93215 M 86 EXCRETION FEW FECES 551, 554 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 568, 575 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 74 512, 519, 526, 533, 540, 547, 551, 554, 561, 568, 575, 582, 589 C93216 U 106 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT VENTRAL-ABDOMINAL-RIGHT 666, 673, 680 631, 638, 645, 652, 659, 694, 701, 708, 715, 722, 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 1233 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 689 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93216 U 106 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729C, 736C WEEK 93 645, 652, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 736C EAR-RIGHT WEEK 61 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610C, 617C, 624C, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 736 VENTRAL-ABDOMINAL-LEFT WEEK 101 701C, 708C, 715C, 722C, 729C, 736C VENTRAL-ABDOMINAL-RIGHT WEEK 79 547C WEEK 81 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C WEEK 96 666, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 736C C93217 D 91 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 635 1234 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 690 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ C93218 M 90 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323 MASS(ES) AXILLARY REGION-LEFT WEEK 50 INGUINAL REGION-LEFT WEEK 48 344, 351, 358, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 330, 337 C93219 U 106 SKIN & PELAGE SORE/SCAB VENTRAL-THORACIC-RIGHT 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43, 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 533, 540, 547, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 554, 582 1235 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 691 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY GROUP: F6 DOSE: 20 PPM ANIMAL DEATH WK OF KEYWORD DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; NUMBER CODE DEATH QUALIFIER 'C' - COMMENTS LISTED AT END OF OBSERVATIONS ------------------------------------------------------------------------------------------------------------------------------------ (CONTINUED FROM PREVIOUS PAGE) C93219 U 106 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 701, 708 VENTRAL-THORACIC-RIGHT WEEK 76 526 WEEK 81 561, 568, 575 WEEK 85 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C C93220 U 106 APPEARANCE MALOCCLUSION 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 736 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 736C 1236 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 692 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93182 F6 708 102 TO 736 106 MASS(ES) AXILLARY REGION-LEFT WEEK 102 15 X 15MM C93183 F6 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 91 25 X 25MM 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 91 30 X 25MM 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 91 40 X 25MM 652 94 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 91 50 X 30MM 666 96 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 91 60 X 40MM 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 91 70 X 40MM 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 91 70 X 50MM 1237 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 693 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93183 F6 694 100 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 91 80 X 50MM 708 102 TO 715 103 MASS(ES) INGUINAL REGION-LEFT WEEK 91 90 X 70MM 722 104 TO 729 105 MASS(ES) INGUINAL REGION-LEFT WEEK 91 110 X 80MM 736 106 TO 736 106 MASS(ES) INGUINAL REGION-LEFT WEEK 91 125 X 100MM C93186 F6 309 45 TO 316 46 MASS(ES) VENTRAL-CERVICAL WEEK 45 20 X 15MM 323 47 TO 330 48 MASS(ES) VENTRAL-CERVICAL WEEK 45 25 X 25MM 337 49 MASS(ES) VENTRAL-CERVICAL WEEK 45 30 X 25MM 344 50 MASS(ES) VENTRAL-CERVICAL WEEK 45 35 X 30MM 1238 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 694 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93186 F6 351 51 TO 379 55 MASS(ES) VENTRAL-CERVICAL WEEK 45 40 X 35MM 386 56 TO 393 57 MASS(ES) VENTRAL-CERVICAL WEEK 45 40 X 40MM 400 58 TO 414 60 MASS(ES) VENTRAL-CERVICAL WEEK 45 50 X 50MM 421 61 TO 435 63 MASS(ES) VENTRAL-CERVICAL WEEK 45 60 X 60MM 442 64 TO 484 70 MASS(ES) VENTRAL-CERVICAL WEEK 45 70 X 70MM 491 71 MASS(ES) VENTRAL-CERVICAL WEEK 45 80 X 70MM 498 72 MASS(ES) VENTRAL-CERVICAL WEEK 45 80 X 80MM 505 73 MASS(ES) VENTRAL-CERVICAL WEEK 45 95 X 80MM 1239 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 695 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93186 F6 512 74 TO 520 75 MASS(ES) VENTRAL-CERVICAL WEEK 45 100 X 110MM C93187 F6 708 102 TO 725 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 102 10 X 10MM 561 81 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 81 15 X 15MM 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 81 20 X 20MM 589 85 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 81 30 X 30MM 603 87 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 81 45 X 40MM 652 94 TO 705 101 MASS(ES) INGUINAL REGION-LEFT WEEK 81 50 X 45MM 708 102 TO 725 104 MASS(ES) INGUINAL REGION-LEFT WEEK 81 65 X 60MM 1240 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 696 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93187 F6 701 101 TO 705 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 101 10 X 10MM C93188 F6 484 70 TO 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 70 25 X 30MM 512 74 TO 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 70 40 X 40MM 526 76 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 70 40 X 50MM 547 79 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 70 55 X 60MM 589 85 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 70 60 X 65MM 603 87 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 70 65 X 70MM 638 92 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 70 75 X 80MM 1241 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 697 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93188 F6 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 70 90 X 85MM 673 97 TO 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 70 95 X 90MM 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 70 80 X 85MM 708 102 TO 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 70 95 X 95MM 722 104 TO 734 105 MASS(ES) AXILLARY REGION-LEFT WEEK 70 100 X 100MM C93192 F6 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 25 X 25MM 638 92 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 30 X 30MM 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 45 X 30MM 1242 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 698 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93192 F6 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 50 X 40MM 687 99 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 50 X 50MM 708 102 TO 729 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 55 X 70MM 736 106 TO 736 106 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 70 X 75MM C93194 F6 568 82 TO 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 81 10 X 10MM 603 87 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 10 X 10MM 561 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 10 X 10MM C93197 F6 694 100 TO 736 106 MASS(ES) AXILLARY REGION-LEFT WEEK 99 10 X 10MM 1243 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 699 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93197 F6 645 93 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 93 10 X 10MM 708 102 TO 722 104 MASS(ES) INGUINAL REGION-LEFT WEEK 93 10 X 20MM 729 105 TO 736 106 MASS(ES) INGUINAL REGION-LEFT WEEK 93 20 X 20MM 659 95 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 95 10 X 10MM POSTERIOR 687 99 TO 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 95 15 X 15MM POSTERIOR 708 102 TO 722 104 MASS(ES) INGUINAL REGION-LEFT WEEK 95 20 X 30MM POSTERIOR 729 105 MASS(ES) INGUINAL REGION-LEFT WEEK 95 30 X 40MM POSTERIOR 736 106 TO 736 106 MASS(ES) INGUINAL REGION-LEFT WEEK 95 40 X 40MM 1244 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 700 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93198 F6 694 100 TO 736 106 MASS(ES) AXILLARY REGION-RIGHT WEEK 94 10 X 10MM C93203 F6 533 77 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 76 10 X 10MM 568 82 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 76 15 X 15MM 610 88 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 76 15 X 25MM 589 85 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 81 20 X 20MM 610 88 TO 624 90 MASS(ES) INGUINAL REGION-LEFT WEEK 81 25 X 30MM 477 69 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 10 X 10MM 512 74 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 20 X 20MM 1245 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 701 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93203 F6 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 30 X 30MM 533 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 20 X 25MM 428 62 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 10 X 10MM 470 68 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 15 X 15MM 484 70 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 20 X 20MM 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 30 X 30MM 519 75 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 25 X 30MM 582 84 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 15 X 20MM 1246 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 702 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93203 F6 561 81 TO 582 84 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 81 20 X 20MM C93204 F6 456 66 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 20 X 20MM 470 68 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 25 X 25MM 477 69 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 30 X 30MM 498 72 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 35 X 30MM 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 35 X 40MM 533 77 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 45 X 45MM 554 80 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 60 X 60MM 1247 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 703 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93204 F6 596 86 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 70 X 60MM 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 70 X 70MM 638 92 TO 642 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 70 X 80MM C93207 F6 71 11 TO 78 12 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 15 X 20MM 85 13 TO 155 23 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 20 X 25MM 162 24 TO 169 25 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 25 X 25MM 176 26 TO 204 30 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 35 X 35MM 211 31 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 45 X 45MM 1248 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 704 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93207 F6 218 32 TO 239 35 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 40 X 40MM 246 36 TO 260 38 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 45 X 50MM 267 39 TO 281 41 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 55 X 55MM C93210 F6 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 94 25 X 25MM 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 94 25 X 30MM 666 96 TO 705 101 MASS(ES) INGUINAL REGION-LEFT WEEK 94 35 X 30MM 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 94 40 X 40MM 715 103 TO 734 105 MASS(ES) INGUINAL REGION-LEFT WEEK 94 50 X 50MM 1249 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 705 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93210 F6 610 88 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 15 X 15MM 631 91 TO 645 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 20 X 20MM 652 94 TO 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 30 X 25MM 673 97 TO 734 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 30 X 35MM C93216 F6 561 81 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 10 X 10MM 589 85 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 20 X 20MM 610 88 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 20 X 25MM 631 91 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 25 X30MM 1250 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 706 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93216 F6 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 30 X 30MM 652 94 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 40 X 40MM 666 96 TO 701 101 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 50 X 40MM 708 102 TO 736 106 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 50 X 60MM 659 95 TO 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 10 X 10MM 694 100 TO 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 15 X 15MM 722 104 TO 736 106 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 20 X 25MM 610 88 TO 624 90 MASS(ES) EAR-RIGHT WEEK 61 10 X 10MM 1251 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 707 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93216 F6 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 15 X 15MM 708 102 TO 736 106 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 25 X 25MM 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 79 10 X 10MM 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 10 X 10MM 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 20 X 20MM 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 20 X 30MM 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 20 X 20MM 673 97 TO 701 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 96 15 X 20MM 1252 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 708 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93216 F6 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 96 20 X 30MM 722 104 TO 729 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 96 20 X 40MM 736 106 TO 736 106 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 96 40 X 40MM C93218 F6 365 53 TO 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 50 10 X 10MM 400 58 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 50 20 X 20MM 582 84 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 50 15 X 15MM 603 87 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 50 20 X 20MM C93219 F6 589 85 TO 708 102 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 85 10 X 10MM 1253 Appendix 2 Covance 6329-183 3M T-6295 Individual Clinical Observations PAGE: 709 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ CATEGORY STUDY KEYWORD ANIMAL GROUP DAY WK QUALIFIER FREE-TEXT COMMENT ------------------------------------------------------------------------------------------------------------------------------------ C93220 F6 673 97 TO 694 100 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 20 X 20MM 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 30 X 20MM 708 102 TO 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 45 X 40MM 722 104 TO 729 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 60 X 50MM 736 106 TO 736 106 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 70 X 60MM 1254 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume IV of IX Page 1255 of 4068 Covance 6329-183 3M T-6295 VOLUME IV OF IX CONTENTS Page APPENDIX 3............................................................................................................ 1257 Individual Body Weight Data (g)........................................................................... 1258 APPENDIX 4............................................................................................................ 1345 Individual Food Consumption Data (g).................................................................. 1346 Individual Test Material Consumption Data (mg/kg/day)....................................... 1432 APPENDIX 5............................................................................................................ 1512 Individual Clinical Hematology Data..................................................................... 1513 Individual Clinical Chemistry Data........................................................................ 1585 Individual Clinical Urinalysis Data........................................................................ 1652 Individual Urine Chemistry Data ........................................................................... 1724 1256 APPENDIX 3 Individual Body Weight Data (g) Covance 6329-183 3M T-6295 1257 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92501 193 257 315 369 415 456 479 514 535 571 C92502 184 235 275 316 349 371 385 406 419 434 C92503 180 237 292 347 380 415 435 464 479 501 C92504 167 221 272 317 343 368 391 432 456 473 C92505 154 201 234 273 298 328 352 379 395 411 C92506 188 247 303 355 C92507 189 251 311 360 391 425 439 463 475 491 C92508 156 213 253 300 325 355 372 405 415 428 C92509 172 231 281 330 369 397 416 446 458 476 C92510 218 276 324 372 404 435 453 479 494 507 C92511 179 239 288 334 367 399 423 456 469 482 C92512 175 232 277 326 352 383 405 434 453 469 C92513 149 206 251 301 335 370 389 413 434 447 C92514 220 269 321 374 415 446 474 504 541 544 C92515 171 213 260 301 340 363 381 408 424 438 C92516 174 225 276 322 354 381 393 414 414 436 C92517 194 259 319 378 430 473 492 529 543 569 C92518 185 244 299 339 380 404 430 459 481 505 C92519 165 222 263 307 338 362 386 413 433 442 C92520 174 227 283 326 355 383 408 434 457 476 C92521 168 220 268 318 339 378 400 417 438 452 C92522 189 243 292 340 368 389 421 433 456 463 C92523 146 202 247 303 336 370 393 421 440 459 C92524 208 276 332 384 C92525 158 217 267 324 366 398 425 451 473 486 C92526 178 240 291 348 C92527 183 239 285 340 375 399 416 449 466 489 C92528 155 206 244 286 318 346 363 387 406 418 C92529 171 222 263 295 C92530 207 267 307 350 380 407 431 456 474 486 C92531 155 210 267 312 341 375 393 420 440 456 1258 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92532 182 248 311 376 417 462 484 526 552 565 C92533 204 278 342 393 430 465 488 520 537 557 C92534 174 232 273 316 357 384 405 429 445 459 C92535 172 235 284 332 372 399 426 455 480 494 C92536 191 248 294 342 375 412 419 445 449 468 C92537 183 253 314 375 422 465 493 527 555 573 C92538 158 217 274 325 364 400 424 451 471 488 C92539 207 271 323 366 410 441 458 499 522 550 C92540 197 256 309 350 390 425 443 474 492 501 C92541 183 251 306 365 406 445 463 501 521 541 C92542 192 251 308 357 385 422 433 463 479 493 C92543 215 274 323 361 385 419 437 462 478 481 C92544 187 250 306 353 390 412 423 455 399 423 C92545 198 255 294 337 367 396 413 443 454 473 C92546 195 250 298 345 C92547 208 271 328 384 432 469 496 528 555 569 C92548 173 231 277 320 341 369 400 420 433 445 C92549 158 207 252 299 327 360 374 403 410 420 C92550 148 198 253 305 352 396 396 440 471 495 C92551 160 217 267 302 339 361 380 409 424 433 C92552 178 234 287 328 366 401 417 451 469 486 C92553 141 186 222 266 275 302 312 335 341 353 C92554 164 219 266 304 335 357 379 404 417 430 C92555 176 225 271 305 332 362 395 422 440 458 C92556 196 247 300 348 381 403 414 440 456 472 C92557 165 221 269 314 353 382 403 430 447 466 C92558 177 238 297 353 392 431 457 492 507 527 C92559 198 257 303 339 367 398 416 442 456 466 C92560 197 256 303 346 373 401 418 451 460 476 C92561 154 208 260 308 351 389 409 431 452 474 C92562 184 246 300 337 371 399 414 439 449 463 1259 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92563 183 243 293 340 374 411 434 457 477 495 C92564 173 229 279 324 364 405 433 462 485 502 C92565 174 230 280 320 346 379 405 437 458 476 C92566 168 230 286 340 382 426 429 467 489 513 C92567 180 241 292 338 378 412 432 464 481 496 C92568 142 196 249 295 332 369 398 428 452 471 C92569 200 256 300 338 374 403 430 457 475 488 C92570 191 259 310 352 385 420 434 463 480 488 GROUP: MALE 2 - 0.5 PPM C92571 168 217 272 322 358 388 414 436 453 480 C92572 160 222 278 328 365 392 427 446 462 480 C92573 190 255 318 373 406 460 495 508 527 554 C92574 195 256 304 358 C92575 184 239 283 310 C92576 213 267 317 364 399 433 455 466 481 504 C92577 169 231 289 344 382 412 422 472 489 511 C92578 179 239 292 332 366 394 431 459 469 482 C92579 209 266 321 373 407 450 479 503 526 543 C92580 169 232 285 332 367 401 434 453 470 494 C92581 187 253 317 368 410 440 466 491 504 526 C92582 145 202 257 298 319 343 366 379 397 414 C92583 185 238 292 322 353 381 402 417 425 446 C92584 159 209 247 278 308 332 351 364 376 394 C92585 204 268 316 353 387 425 455 475 491 509 C92586 161 208 262 307 331 372 405 427 446 468 C92587 178 240 297 344 383 422 452 493 508 530 C92588 184 235 285 322 359 390 419 442 455 471 C92589 179 242 303 355 394 432 459 486 498 517 C92590 178 244 309 361 406 437 470 495 506 530 1260 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92591 176 229 275 311 351 373 392 409 424 441 C92592 143 208 268 316 369 395 427 456 474 497 C92593 195 251 310 342 389 406 428 450 454 470 C92594 181 238 293 334 379 402 427 441 456 475 C92595 180 238 293 332 370 398 423 449 453 468 C92596 199 253 297 330 362 391 417 433 445 465 C92597 188 240 287 320 355 382 397 412 422 438 C92598 171 230 283 329 366 395 425 447 465 479 C92599 219 285 341 382 420 462 488 510 517 545 C92600 176 228 275 312 351 382 406 429 440 457 C92601 149 206 259 305 349 376 403 423 436 450 C92602 169 224 267 302 341 365 381 399 403 418 C92603 196 259 312 356 396 421 443 462 472 490 C92604 200 252 304 343 381 405 432 458 472 489 C92605 202 263 311 348 388 420 451 471 486 508 C92606 152 209 260 305 343 375 395 420 436 455 C92607 157 219 283 336 381 415 438 468 488 507 C92608 210 272 320 371 406 454 470 495 505 526 C92609 179 239 298 334 373 403 428 448 457 473 C92610 187 249 301 336 C92611 190 245 300 333 373 396 415 432 444 462 C92612 159 215 268 316 359 392 414 441 455 481 C92613 171 230 294 332 C92614 197 260 317 353 396 426 450 477 490 514 C92615 168 231 284 327 361 391 424 448 459 475 C92616 144 189 235 266 295 323 342 361 374 389 C92617 184 249 309 359 409 447 483 513 528 552 C92618 165 225 284 338 C92619 174 230 279 312 347 374 401 414 421 439 C92620 171 217 261 295 329 358 380 394 407 418 C92621 193 254 304 338 380 413 439 460 474 497 1261 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92622 145 210 269 322 364 399 431 452 466 473 C92623 162 214 259 297 326 359 380 402 386 416 C92624 202 255 297 331 351 379 401 418 438 451 C92625 159 223 288 351 393 443 476 499 516 545 C92626 173 236 287 335 379 414 440 463 488 510 C92627 191 257 312 354 392 427 453 474 494 515 C92628 194 258 312 351 394 422 457 474 494 518 C92629 149 215 274 321 364 400 430 454 473 497 C92630 193 259 315 359 395 427 455 475 484 501 GROUP: MALE 3 - 2 PPM C92631 186 240 290 323 361 384 403 425 435 456 C92632 220 290 344 396 435 469 496 528 553 577 C92633 159 213 257 270 312 349 380 411 426 449 C92634 147 199 248 288 328 357 377 397 413 430 C92635 178 245 303 349 387 418 437 467 471 497 C92636 197 263 308 342 373 401 413 437 440 466 C92637 169 218 257 287 319 346 361 380 384 400 C92638 184 248 292 343 389 435 466 503 516 555 C92639 169 219 259 301 335 370 401 425 438 464 C92640 191 248 286 319 344 366 377 393 397 410 C92641 179 233 284 323 367 398 425 452 459 488 C92642 201 254 304 344 386 413 432 458 459 486 C92643 151 211 256 304 339 359 374 403 407 430 C92644 185 245 295 333 365 382 394 406 408 420 C92645 176 238 281 319 361 390 415 432 442 463 C92646 226 282 329 369 C92647 188 244 291 328 358 384 396 419 421 454 C92648 176 228 269 304 335 355 368 386 385 405 C92649 158 203 246 279 309 336 353 375 375 397 1262 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92650 158 215 271 315 C92651 196 260 312 355 394 428 443 470 480 509 C92652 156 213 265 313 C92653 173 230 284 330 375 404 423 453 464 494 C92654 171 233 282 335 376 400 421 459 456 483 C92655 157 218 277 328 374 407 438 474 489 514 C92656 162 220 273 322 376 415 435 474 476 514 C92657 176 234 286 333 367 402 421 447 451 481 C92658 192 249 297 344 383 411 437 464 470 499 C92659 206 269 319 358 402 430 449 477 480 508 C92660 195 257 299 339 375 395 419 434 436 454 C92661 205 260 302 341 377 404 419 439 448 464 C92662 164 214 261 299 335 362 379 404 410 435 C92663 171 233 292 350 401 428 443 479 483 507 C92664 197 256 310 349 392 416 435 462 469 490 C92665 187 252 309 356 402 433 457 487 503 525 C92666 199 257 310 358 399 435 455 477 483 506 C92667 181 243 298 344 392 425 452 484 490 523 C92668 190 243 289 332 C92669 167 223 268 307 345 376 398 419 422 447 C92670 165 224 274 316 351 381 389 415 417 429 C92671 146 200 252 306 347 376 402 400 417 446 C92672 153 209 252 295 333 346 380 411 424 443 C92673 200 262 307 351 385 423 433 459 469 489 C92674 174 237 277 310 339 368 393 425 431 456 C92675 189 250 299 340 373 404 413 437 433 459 C92676 197 259 293 332 352 380 404 425 430 449 C92677 171 229 273 317 351 384 411 430 430 452 C92678 140 195 243 293 C92679 180 237 285 331 374 407 425 451 464 487 C92680 209 269 323 365 403 439 462 490 500 524 1263 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92681 164 218 270 315 353 382 410 433 441 464 C92682 171 224 280 325 357 385 407 425 438 453 C92683 180 241 287 336 374 407 426 449 457 480 C92684 183 237 277 315 343 374 393 414 420 436 C92685 160 219 265 318 361 398 424 455 468 493 C92686 193 250 300 342 372 402 426 457 466 475 C92687 206 264 305 341 388 421 447 478 490 511 C92688 176 228 268 308 344 375 391 414 424 448 C92689 155 216 267 319 360 395 419 445 445 450 C92690 201 276 343 405 458 486 516 552 567 587 GROUP: MALE 4 - 5 PPM C92691 163 214 277 314 349 378 408 432 445 457 C92692 146 199 258 313 350 383 415 448 463 474 C92693 175 227 285 327 365 401 426 451 468 480 C92694 194 249 296 336 366 402 427 458 482 498 C92695 202 260 314 355 383 414 439 462 468 476 C92696 204 261 308 349 379 412 436 455 472 481 C92697 183 242 293 334 366 399 428 447 468 485 C92698 188 254 304 345 389 425 450 471 488 498 C92699 162 214 251 287 317 346 372 374 396 412 C92700 171 219 257 288 312 333 352 362 376 385 C92701 191 249 297 339 368 404 422 447 463 484 C92702 157 216 267 312 349 379 404 431 443 457 C92703 150 209 264 313 356 391 425 448 473 489 C92704 177 236 282 329 367 401 425 454 475 489 C92705 168 222 272 311 350 383 400 431 448 458 C92706 174 235 289 327 362 398 424 445 466 482 C92707 181 235 284 324 350 377 396 416 427 442 C92708 177 239 294 344 370 386 406 425 435 450 1264 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92709 199 255 297 325 354 387 413 443 456 463 C92710 162 217 267 311 349 386 418 442 456 472 C92711 179 229 282 319 350 385 414 440 460 473 C92712 192 257 321 361 398 435 467 482 503 516 C92713 217 280 345 393 431 458 475 501 517 527 C92714 176 233 300 346 379 407 432 455 480 499 C92715 194 249 310 349 C92716 211 269 336 380 414 452 481 515 534 547 C92717 164 224 286 329 369 398 424 442 462 475 C92718 156 212 266 305 C92719 139 190 251 299 333 362 391 415 428 436 C92720 189 248 321 363 397 434 460 480 498 514 C92721 186 236 292 333 326 353 378 392 412 425 C92722 194 248 304 347 380 417 439 459 473 490 C92723 188 242 307 352 393 423 430 476 492 513 C92724 206 264 330 374 413 448 481 503 523 527 C92725 165 217 274 312 330 365 386 408 416 428 C92726 168 224 284 331 370 403 431 453 474 484 C92727 182 233 286 330 357 391 419 441 459 470 C92728 162 227 284 348 401 437 478 507 531 544 C92729 201 263 331 384 428 463 493 516 532 542 C92730 158 215 268 308 339 374 397 419 439 448 C92731 199 260 309 336 C92732 147 205 264 312 343 376 406 431 446 464 C92733 173 231 280 317 346 366 388 401 410 429 C92734 178 241 293 333 C92735 217 273 321 354 391 421 444 464 479 492 C92736 175 229 281 318 352 381 400 422 439 454 C92737 183 240 290 328 363 391 415 431 450 467 C92738 141 198 254 302 335 368 394 420 438 463 C92739 164 215 259 292 319 345 358 379 397 409 1265 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92740 173 229 290 337 370 398 425 455 479 489 C92741 198 252 301 339 378 410 438 454 468 480 C92742 178 245 308 364 404 448 478 508 535 551 C92743 159 224 289 340 386 428 458 486 516 530 C92744 154 211 266 318 C92745 198 252 304 356 389 429 454 480 499 516 C92746 209 272 332 381 409 452 474 502 519 533 C92747 184 234 283 321 356 384 418 434 453 464 C92748 193 252 302 350 387 415 452 480 497 515 C92749 154 207 254 289 323 355 378 401 417 430 C92750 189 248 301 340 374 406 429 443 463 470 GROUP: MALE 5 - 20 PPM C92751 164 219 275 317 364 400 421 454 468 490 C92752 167 223 263 295 C92753 206 267 320 358 406 435 444 474 494 511 C92754 178 220 267 299 329 353 373 389 394 411 C92755 146 200 249 294 325 350 372 394 406 435 C92756 191 254 312 354 403 439 467 488 500 522 C92757 164 207 251 286 320 349 373 388 406 416 C92758 149 207 259 303 343 376 400 420 436 449 C92759 197 249 301 343 C92760 196 252 303 344 382 407 424 455 464 476 C92761 183 237 289 323 358 383 406 417 431 441 C92762 183 238 288 324 367 397 426 446 458 468 C92763 182 246 307 355 395 431 463 488 511 529 C92764 197 264 327 369 398 435 456 470 494 513 C92765 185 240 295 335 378 409 434 453 470 487 C92766 171 230 282 318 356 385 403 420 432 449 C92767 168 233 293 345 388 411 450 475 503 525 1266 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92768 209 269 331 377 417 455 484 504 509 531 C92769 182 240 297 339 374 405 428 456 476 494 C92770 217 283 339 374 410 441 462 486 507 522 C92771 165 226 280 328 364 396 427 451 464 484 C92772 175 231 288 337 366 398 423 437 454 476 C92773 180 235 282 319 351 375 386 406 419 433 C92774 166 226 281 317 354 389 416 443 456 475 C92775 155 220 277 332 372 409 437 464 482 495 C92776 189 253 308 356 392 418 432 455 477 491 C92777 151 205 254 291 330 349 370 390 404 420 C92778 202 265 321 362 407 429 446 466 480 491 C92779 160 217 258 295 C92780 159 211 250 280 310 336 356 374 392 408 C92781 183 241 288 320 362 385 407 423 433 455 C92782 138 196 247 295 334 363 382 407 428 452 C92783 200 266 319 360 402 436 464 482 494 517 C92784 183 242 288 334 361 394 415 425 430 455 C92785 190 239 290 324 359 387 411 428 439 457 C92786 172 232 280 317 356 383 412 427 440 459 C92787 206 260 308 342 383 406 425 438 452 471 C92788 181 248 310 354 387 418 445 460 474 502 C92789 155 211 262 303 338 369 387 408 433 447 C92790 150 216 266 312 352 384 409 434 447 471 C92791 179 229 272 307 346 372 393 405 417 438 C92792 186 243 297 337 380 409 427 447 463 487 C92793 192 247 290 325 C92794 187 237 279 309 341 367 385 400 413 433 C92795 168 223 269 300 337 363 384 415 434 452 C92796 171 220 260 290 324 350 377 390 404 422 C92797 201 249 297 324 356 376 395 409 422 445 C92798 162 223 273 315 1267 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92799 181 239 282 317 357 393 431 452 472 491 C92800 184 243 293 327 361 390 409 429 438 462 C92801 210 266 318 359 407 441 468 486 509 533 C92802 173 231 281 314 350 377 405 423 433 451 C92803 200 257 311 341 384 416 446 459 470 492 C92804 169 222 263 299 336 367 391 399 418 441 C92805 194 259 310 352 382 418 448 469 482 504 C92806 164 223 283 326 374 402 426 448 416 433 C92807 152 210 261 300 333 366 398 421 439 456 C92808 150 202 250 292 328 355 374 391 405 422 C92809 170 226 282 319 358 389 411 431 447 471 C92810 204 257 304 334 368 393 412 426 441 454 C92811 167 225 282 324 351 395 415 440 446 478 C92812 182 240 293 326 365 395 428 449 455 481 C92813 183 250 300 334 374 400 423 446 453 470 C92814 146 196 242 276 305 325 343 354 359 372 C92815 204 249 283 302 334 361 344 370 379 409 C92816 141 194 237 276 307 342 366 385 396 415 C92817 187 248 300 347 393 432 461 481 500 532 C92818 189 245 291 319 357 382 402 414 422 453 C92819 155 204 244 274 301 327 352 371 381 398 C92820 186 229 270 299 331 358 380 396 398 419 GROUP: MALE 6 - 20 PPM C92821 144 193 244 291 323 360 389 413 417 437 C92822 165 214 266 305 340 369 391 414 419 438 C92823 181 237 293 333 368 400 418 432 444 460 C92824 200 255 298 335 373 401 429 453 460 484 C92825 183 248 310 361 408 444 476 498 513 535 C92826 181 224 272 307 340 371 387 406 412 435 1268 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92827 192 236 284 322 353 385 408 427 435 456 C92828 184 247 300 342 380 412 437 460 472 493 C92829 187 241 293 332 376 418 440 463 480 507 C92830 158 210 259 294 330 361 370 390 404 425 C92831 202 258 318 353 389 418 438 457 475 488 C92832 153 200 257 299 334 360 385 400 415 426 C92833 164 220 279 331 367 403 427 458 478 497 C92834 177 225 280 326 364 392 412 431 442 463 C92835 168 221 273 301 332 362 378 400 409 431 C92836 213 268 329 370 405 436 449 477 446 476 C92837 175 233 301 347 395 433 457 478 494 509 C92838 172 226 282 319 359 384 407 424 435 458 C92839 187 254 329 392 441 490 521 554 559 586 C92840 187 236 282 315 340 366 384 403 407 419 C92841 185 227 281 314 350 376 389 407 414 431 C92842 213 275 340 378 421 443 466 490 495 511 C92843 210 273 331 371 407 434 458 478 490 512 C92844 152 199 248 281 306 329 347 363 368 379 C92845 193 237 285 320 351 373 392 411 418 441 C92846 166 224 281 321 364 396 423 451 468 489 C92847 170 227 298 349 397 436 465 496 512 538 C92848 135 178 221 252 283 306 328 352 358 383 C92849 173 226 287 332 368 400 424 449 454 477 C92850 205 250 296 321 359 379 396 411 420 435 C92851 173 222 283 325 368 403 419 445 457 475 C92852 156 207 263 299 339 370 388 411 412 439 C92853 192 242 292 325 357 383 403 422 434 454 C92854 193 254 317 352 386 420 430 460 460 475 C92855 175 229 284 323 359 387 406 370 405 431 C92856 151 200 262 301 339 367 388 413 422 437 C92857 189 247 308 355 395 426 447 476 487 506 1269 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92858 164 218 275 327 371 404 430 456 470 496 C92859 191 240 295 333 365 399 426 451 458 468 C92860 158 205 256 296 337 356 379 398 406 423 GROUP: FEMALE 1 - 0 PPM C92861 176 204 239 251 C92862 134 153 174 191 198 213 217 226 229 235 C92863 147 179 202 218 232 247 244 264 267 277 C92864 156 193 223 245 C92865 151 189 211 226 241 249 257 273 262 276 C92866 170 183 218 231 239 245 249 253 268 285 C92867 153 186 209 235 265 263 265 290 286 302 C92868 147 170 194 208 237 241 247 255 266 281 C92869 140 157 181 199 209 215 216 224 223 237 C92870 141 168 192 209 215 231 228 243 246 256 C92871 161 192 218 229 250 254 264 273 278 278 C92872 141 168 181 193 200 210 206 215 218 219 C92873 149 164 173 195 211 213 207 221 224 225 C92874 146 186 206 217 240 251 255 261 267 277 C92875 142 171 184 194 214 215 232 242 241 255 C92876 144 175 192 210 220 233 234 246 247 254 C92877 150 174 196 214 224 223 228 234 244 251 C92878 143 178 207 216 225 227 241 248 249 244 C92879 167 198 222 240 249 260 269 285 289 306 C92880 157 185 205 212 219 234 245 254 254 261 C92881 154 184 210 221 236 249 255 267 270 278 C92882 146 168 190 205 206 216 222 230 232 243 C92883 171 210 241 258 273 284 290 312 313 325 C92884 154 190 210 219 234 241 236 254 257 267 C92885 163 194 217 235 245 256 263 274 279 294 1270 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92886 149 180 199 201 209 227 231 235 234 252 C92887 159 188 214 233 238 266 263 258 259 267 C92888 165 191 232 254 269 282 302 304 317 324 C92889 141 167 190 202 218 231 235 240 242 254 C92890 176 216 246 267 275 291 297 307 301 322 C92891 144 173 199 212 218 230 240 245 246 256 C92892 161 198 226 249 278 302 311 329 323 344 C92893 151 176 201 215 216 233 235 244 237 250 C92894 162 183 206 217 227 227 232 241 249 248 C92895 165 190 202 231 246 255 252 271 269 272 C92896 140 173 199 217 226 244 249 262 258 275 C92897 142 167 188 197 204 214 223 237 234 244 C92898 146 181 202 218 236 248 253 262 270 280 C92899 163 200 225 258 279 298 307 330 323 351 C92900 168 195 223 240 250 263 274 288 294 303 C92901 143 171 190 206 225 235 235 254 245 259 C92902 168 200 232 254 272 289 301 334 348 362 C92903 182 232 257 283 299 300 306 329 332 342 C92904 145 173 184 202 211 227 225 244 241 255 C92905 143 175 193 212 224 235 238 250 253 261 C92906 138 168 193 210 212 227 232 240 241 251 C92907 143 169 189 210 227 229 242 249 251 266 C92908 152 182 207 211 238 249 251 263 272 286 C92909 156 178 199 215 229 234 243 262 262 268 C92910 156 182 211 227 250 269 272 296 292 310 C92911 146 167 184 189 212 210 218 233 227 248 C92912 155 184 211 232 240 264 267 286 286 296 C92913 161 190 217 236 242 253 257 271 275 286 C92914 149 170 190 192 C92915 150 163 191 209 231 239 249 268 259 262 C92916 166 196 236 264 278 289 304 319 331 328 1271 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92917 164 204 226 264 273 290 302 306 324 339 C92918 167 196 214 229 240 253 256 265 267 271 C92919 153 187 219 265 C92920 139 163 178 198 213 217 227 237 235 247 C92921 165 202 217 239 256 271 285 301 309 321 C92922 155 187 224 228 235 253 250 275 272 289 C92923 133 161 175 196 214 222 225 239 242 241 C92924 158 187 205 226 248 259 269 283 287 294 C92925 156 191 218 243 252 275 282 302 303 315 C92926 168 195 219 228 C92927 159 180 205 213 234 241 255 260 261 280 C92928 163 195 215 221 250 259 263 266 274 290 C92929 152 174 202 216 229 238 247 260 253 266 C92930 172 199 246 270 297 305 324 330 335 352 GROUP: FEMALE 2 - 0.5 PPM C92931 156 191 220 240 260 273 282 292 309 316 C92932 150 184 207 221 228 242 246 259 256 269 C92933 151 173 192 211 231 252 268 277 293 315 C92934 148 170 189 200 215 221 231 244 247 250 C92935 150 176 198 219 241 252 259 270 279 295 C92936 138 169 195 210 214 227 232 247 245 259 C92937 150 180 195 217 229 239 250 257 262 273 C92938 156 180 200 213 243 252 258 280 283 287 C92939 152 166 179 190 206 223 216 234 233 240 C92940 144 165 187 202 216 224 228 238 242 255 C92941 156 184 207 229 C92942 158 194 218 237 245 260 271 288 287 305 C92943 162 189 214 239 253 279 282 298 302 305 C92944 173 204 236 252 283 304 309 320 332 347 1272 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92945 146 161 193 207 C92946 140 157 184 194 201 207 225 235 242 251 C92947 150 179 194 221 236 256 263 269 279 293 C92948 161 175 212 235 252 283 273 299 315 319 C92949 175 216 245 289 291 311 346 357 359 387 C92950 156 180 193 221 C92951 153 174 189 195 202 216 222 238 233 248 C92952 141 163 181 195 218 223 230 248 248 260 C92953 147 175 189 210 217 229 237 246 245 256 C92954 141 163 195 209 229 244 245 261 262 270 C92955 157 188 210 226 245 259 257 280 276 301 C92956 165 196 216 246 259 271 273 289 298 311 C92957 148 177 198 209 216 229 226 246 246 248 C92958 146 161 181 188 205 218 218 224 233 244 C92959 167 201 231 245 255 272 269 286 295 313 C92960 165 192 235 255 270 272 285 291 302 294 C92961 181 226 247 263 302 313 319 332 331 342 C92962 157 178 205 210 222 234 232 241 249 255 C92963 150 169 187 194 205 217 220 224 231 245 C92964 165 189 208 231 226 246 242 250 254 268 C92965 141 175 199 211 234 242 247 256 261 271 C92966 142 167 186 198 210 223 226 235 234 241 C92967 144 173 202 224 241 256 263 274 276 290 C92968 151 186 215 241 242 263 274 283 288 301 C92969 149 167 183 197 C92970 161 187 218 235 260 273 280 289 305 328 C92971 148 155 185 200 215 224 226 236 232 245 C92972 145 169 201 215 235 242 250 254 267 269 C92973 143 153 184 199 214 223 235 243 241 254 C92974 153 175 186 209 223 231 232 245 248 256 C92975 150 175 197 216 222 242 242 260 254 265 1273 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92976 178 204 233 253 264 277 279 296 303 310 C92977 140 165 191 216 221 248 265 275 275 287 C92978 170 208 237 270 278 304 305 324 334 339 C92979 147 173 191 209 229 242 246 259 262 270 C92980 147 171 193 217 231 253 259 267 278 285 C92981 162 192 227 244 248 259 263 278 283 289 C92982 153 176 202 211 C92983 140 180 205 222 237 261 283 304 293 301 C92984 168 198 221 241 259 275 282 295 306 314 C92985 163 193 216 242 261 266 284 294 306 308 C92986 144 165 191 205 222 229 241 252 250 257 C92987 164 186 220 235 245 248 262 269 272 275 C92988 154 168 200 215 230 238 246 261 263 263 C92989 151 176 203 222 225 238 247 251 258 261 C92990 171 196 226 235 242 254 263 274 275 269 GROUP: FEMALE 3 - 2 PPM C92991 142 155 185 202 212 216 228 241 245 249 C92992 158 185 202 214 231 241 244 254 260 259 C92993 156 182 203 218 227 246 260 270 272 284 C92994 153 170 184 204 224 234 246 244 256 264 C92995 154 177 204 215 241 248 250 267 264 C92996 157 172 205 221 C92997 160 177 197 206 225 238 237 247 245 262 C92998 159 176 198 207 225 243 242 266 270 280 C92999 156 189 215 232 248 262 256 280 285 287 C93000 176 193 215 234 247 252 257 266 260 276 C93001 141 163 185 196 212 228 232 235 243 251 C93002 158 179 203 221 221 239 236 245 252 247 C93003 163 183 212 218 251 268 269 283 290 308 1274 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93004 150 170 194 206 223 226 235 261 252 268 C93005 160 184 197 218 225 230 241 247 251 248 C93006 160 175 206 212 C93007 154 178 196 208 228 239 240 247 250 258 C93008 173 192 218 240 C93009 150 165 186 201 218 232 245 255 258 268 C93010 152 183 207 225 243 250 255 264 267 271 C93011 156 186 210 234 257 263 269 272 280 286 C93012 167 192 228 236 271 290 289 317 308 319 C93013 139 148 177 190 198 214 219 238 231 233 C93014 165 200 221 247 270 296 287 303 295 308 C93015 165 187 215 237 248 260 268 284 286 291 C93016 135 144 160 166 191 199 212 220 225 226 C93017 152 162 184 197 218 224 235 248 248 260 C93018 163 180 209 233 237 250 260 273 273 278 C93019 163 188 213 234 248 263 269 279 289 292 C93020 156 167 203 228 239 248 258 266 270 280 C93021 141 171 191 220 236 251 259 276 274 279 C93022 164 187 210 230 246 262 261 285 281 295 C93023 144 155 174 196 208 214 227 239 241 243 C93024 163 193 206 234 248 257 257 268 266 269 C93025 174 198 220 250 255 270 286 300 303 315 C93026 141 161 188 206 221 240 254 261 260 273 C93027 156 179 203 224 231 246 256 264 261 271 C93028 156 178 200 219 224 237 247 261 260 261 C93029 146 162 191 200 223 228 235 244 239 234 C93030 146 169 193 204 225 238 242 238 248 268 C93031 143 162 184 203 C93032 152 171 192 217 218 234 233 246 250 259 C93033 168 188 196 224 226 243 248 264 265 269 C93034 168 195 207 238 237 259 269 269 270 278 1275 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93035 151 179 207 226 238 257 266 273 274 287 C93036 157 173 191 216 222 237 246 253 259 267 C93037 143 168 188 216 236 244 258 265 264 276 C93038 140 156 170 192 197 215 216 218 222 236 C93039 144 160 188 202 228 244 254 272 274 281 C93040 134 151 162 180 188 205 212 213 223 230 C93041 158 182 196 219 235 240 254 263 267 273 C93042 163 194 216 244 261 276 272 294 291 292 C93043 148 163 195 208 226 248 245 269 267 273 C93044 154 168 195 208 229 232 239 253 256 257 C93045 162 200 221 243 C93046 164 201 237 268 278 291 300 303 309 325 C93047 171 200 231 249 282 299 298 316 314 326 C93048 140 157 177 182 203 211 220 218 223 234 C93049 151 169 195 210 216 227 237 241 239 244 C93050 163 178 200 220 234 244 250 258 260 265 GROUP: FEMALE 4 - 5 PPM C93051 150 165 196 196 223 222 234 234 251 250 C93052 151 178 202 211 231 246 250 260 263 277 C93053 151 169 194 202 215 229 228 248 248 264 C93054 150 160 178 187 205 219 224 239 238 249 C93055 156 185 208 223 234 251 256 272 269 286 C93056 145 179 208 222 231 248 256 268 265 278 C93057 140 162 186 198 218 231 230 241 236 245 C93058 169 210 233 257 C93059 150 181 203 223 233 250 254 269 267 274 C93060 161 185 198 219 238 249 247 275 276 280 C93061 159 184 200 226 238 249 252 269 267 278 C93062 146 165 189 198 215 229 234 244 253 264 1276 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93063 156 179 213 213 245 256 254 263 260 264 C93064 144 169 191 202 217 227 230 235 237 245 C93065 128 150 169 172 190 197 197 203 210 221 C93066 162 192 216 232 234 253 251 270 257 265 C93067 162 190 199 231 C93068 158 174 200 220 222 237 246 254 254 264 C93069 168 207 237 267 275 288 296 303 309 326 C93070 164 192 219 236 C93071 146 167 182 195 201 200 210 221 219 233 C93072 143 173 198 213 219 230 240 246 251 259 C93073 157 179 204 214 222 240 247 259 258 272 C93074 161 192 232 245 253 263 261 279 276 290 C93075 155 174 192 205 C93076 146 172 192 214 225 255 241 257 264 265 C93077 164 197 220 242 255 261 262 284 283 296 C93078 150 171 195 205 215 232 246 254 247 261 C93079 159 188 212 225 254 250 267 286 310 299 C93080 146 171 207 218 238 251 253 265 259 266 C93081 143 167 177 198 205 223 233 240 244 248 C93082 169 196 208 230 254 264 265 289 291 294 C93083 155 181 208 221 231 251 251 265 265 277 C93084 144 167 193 206 C93085 155 182 209 213 234 245 251 262 261 273 C93086 164 190 212 236 249 260 267 281 281 283 C93087 135 163 186 189 204 214 220 230 235 236 C93088 154 177 200 214 232 237 242 253 249 254 C93089 173 210 233 253 259 267 278 286 290 296 C93090 154 183 205 217 226 234 237 241 250 250 C93091 168 198 221 236 261 258 277 289 278 302 C93092 152 176 196 212 216 231 233 248 248 262 C93093 159 194 216 219 255 269 277 295 302 325 1277 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93094 132 159 188 202 215 230 257 257 254 271 C93095 143 177 200 212 225 233 238 249 246 261 C93096 145 176 209 236 248 258 274 294 295 308 C93097 154 166 183 188 209 221 230 246 247 256 C93098 143 168 190 206 218 224 231 242 247 256 C93099 153 176 207 221 235 248 251 255 260 274 C93100 170 196 217 231 242 244 251 265 263 284 C93101 156 170 196 204 219 218 233 245 244 263 C93102 165 200 230 241 260 269 284 290 296 310 C93103 151 184 200 225 223 252 249 263 273 278 C93104 171 199 229 245 267 285 304 328 337 361 C93105 158 188 227 233 261 278 263 278 277 292 C93106 158 178 201 212 227 228 236 253 247 253 C93107 143 172 198 210 228 243 249 260 268 280 C93108 148 166 191 197 213 219 227 244 244 264 C93109 150 174 200 207 228 245 244 254 257 272 C93110 173 193 234 261 288 307 316 341 343 361 GROUP: FEMALE 5 - 20 PPM C93111 160 192 224 257 261 275 277 295 295 306 C93112 175 191 221 237 240 259 265 264 264 265 C93113 139 155 161 177 194 199 200 217 220 219 C93114 162 194 219 222 C93115 151 159 178 189 193 203 203 210 209 218 C93116 146 180 201 218 216 225 231 240 233 245 C93117 138 167 179 206 215 219 228 242 236 256 C93118 152 164 185 199 218 218 214 229 223 233 C93119 148 171 183 187 204 205 202 215 206 205 C93120 144 182 195 214 216 225 227 236 235 233 C93121 132 155 166 180 187 191 197 212 206 213 1278 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93122 167 194 229 232 261 272 266 273 282 293 C93123 166 192 212 228 C93124 152 183 200 209 222 236 236 244 235 243 C93125 140 152 169 182 193 198 199 213 215 210 C93126 139 159 172 191 196 211 204 217 217 226 C93127 156 184 203 207 227 240 234 242 242 250 C93128 161 175 201 202 219 222 227 243 238 250 C93129 144 168 192 216 236 239 245 250 251 263 C93130 159 182 209 227 241 241 245 261 257 272 C93131 160 187 215 229 239 250 256 264 270 275 C93132 158 192 214 230 238 243 247 252 241 256 C93133 152 184 204 205 220 225 223 234 230 240 C93134 180 214 223 234 246 255 265 268 274 277 C93135 144 180 201 225 230 247 244 260 252 265 C93136 161 190 208 208 225 238 240 242 244 246 C93137 144 160 188 201 205 212 219 233 230 229 C93138 163 184 207 220 227 234 239 252 247 262 C93139 139 166 179 199 207 212 206 214 213 223 C93140 146 175 199 229 227 246 245 255 260 266 C93141 150 182 209 231 240 259 263 273 271 278 C93142 171 198 210 238 C93143 169 191 222 238 238 257 266 276 277 274 C93144 137 149 176 184 C93145 135 160 173 194 203 210 213 217 214 226 C93146 147 172 187 201 196 204 207 220 211 225 C93147 146 174 196 209 226 231 236 246 244 254 C93148 146 179 205 222 226 234 249 266 266 287 C93149 145 159 185 197 210 223 224 234 235 244 C93150 171 196 229 235 248 263 258 266 266 273 C93151 157 188 207 223 225 234 239 249 245 255 C93152 160 189 207 227 235 241 246 261 260 268 1279 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93153 168 194 204 226 230 236 233 246 241 236 C93154 150 179 205 220 226 243 246 252 251 264 C93155 151 168 196 220 220 221 222 244 231 236 C93156 139 164 180 201 201 211 218 227 223 236 C93157 161 181 192 221 223 229 229 244 243 255 C93158 147 175 200 212 225 234 235 240 251 260 C93159 151 168 189 204 C93160 151 183 215 239 247 262 276 286 286 301 C93161 144 177 200 226 233 241 251 257 257 273 C93162 175 206 236 246 243 258 262 279 277 271 C93163 146 168 191 211 220 223 226 237 234 239 C93164 160 170 195 192 209 208 220 221 224 237 C93165 147 177 193 210 216 229 230 243 241 250 C93166 167 197 226 237 267 272 266 291 297 300 C93167 160 172 213 233 237 257 252 268 276 270 C93168 144 157 189 201 220 231 229 241 234 243 C93169 157 177 197 209 229 234 237 242 237 251 C93170 156 180 202 220 228 251 254 261 257 268 C93171 156 181 191 220 226 238 247 253 256 262 C93172 157 180 199 216 232 236 241 258 254 246 C93173 148 174 197 219 220 224 232 242 237 257 C93174 155 169 188 200 202 221 231 236 235 239 C93175 144 173 198 208 217 225 227 240 234 251 C93176 130 160 183 199 215 227 229 238 236 247 C93177 158 172 189 198 211 220 222 233 229 235 C93178 149 176 199 213 231 233 236 244 241 250 C93179 145 171 191 205 209 219 226 232 229 240 C93180 174 201 229 250 257 270 274 283 281 292 1280 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93181 151 170 187 203 208 220 233 248 240 249 C93182 150 171 192 205 211 217 222 238 231 250 C93183 161 184 197 227 239 248 262 275 276 285 C93184 134 159 168 194 206 218 215 229 227 231 C93185 163 181 204 222 229 240 245 253 250 261 C93186 156 162 182 200 210 201 215 221 217 213 C93187 157 180 200 204 232 234 242 251 252 256 C93188 160 188 208 231 237 245 246 260 263 272 C93189 147 171 192 197 213 216 222 231 228 240 C93190 152 181 199 220 229 243 244 257 251 262 C93191 167 191 231 244 260 276 278 298 306 306 C93192 161 179 198 203 218 220 225 227 231 232 C93193 157 173 199 217 229 233 240 246 245 240 C93194 173 202 220 246 264 271 274 289 285 297 C93195 170 192 218 237 244 252 262 274 266 270 C93196 135 152 174 179 199 200 211 216 213 224 C93197 151 167 181 188 201 205 210 213 215 225 C93198 157 179 197 215 236 239 247 250 245 257 C93199 161 189 205 221 226 233 235 245 243 248 C93200 151 167 189 198 212 224 230 241 231 241 C93201 133 158 183 202 209 222 228 237 230 238 C93202 140 153 194 207 228 234 236 248 249 239 C93203 156 183 208 220 245 241 258 266 262 271 C93204 156 191 201 226 236 234 242 254 246 245 C93205 179 205 221 249 254 265 269 276 273 282 C93206 148 159 176 191 195 213 216 227 220 232 C93207 139 161 184 195 210 222 222 228 229 238 C93208 163 187 197 225 223 238 234 246 241 249 C93209 168 190 201 202 224 240 228 238 238 244 C93210 161 193 210 213 239 245 257 257 263 266 C93211 145 165 173 198 203 216 217 227 223 231 1281 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93212 146 172 204 205 231 237 246 263 259 262 C93213 157 186 202 223 234 236 245 253 251 257 C93214 148 166 193 198 213 222 226 242 231 244 C93215 145 169 198 219 233 241 249 256 255 264 C93216 142 169 188 207 213 227 236 242 238 252 C93217 162 193 220 240 259 270 267 286 283 291 C93218 136 162 181 201 207 215 221 235 230 236 C93219 181 214 242 266 276 285 275 297 293 299 C93220 166 192 207 229 247 250 253 257 267 276 1282 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92501 591 603 615 641 639 657 667 698 725 762 C92502 452 460 463 469 474 484 486 509 522 548 C92503 512 511 517 523 529 547 549 560 592 630 C92504 494 475 466 474 476 487 491 523 555 572 C92505 422 419 432 439 447 460 468 488 504 530 C92507 506 507 522 526 532 539 541 557 571 590 C92508 443 454 460 469 471 481 492 517 540 574 C92509 499 502 515 523 C92510 528 531 541 555 568 572 575 602 616 627 C92511 500 515 526 541 C92512 486 498 505 520 525 547 552 585 607 625 C92513 466 477 489 496 506 520 524 544 576 609 C92514 577 593 603 620 640 658 668 719 753 806 C92515 457 470 479 485 495 515 516 541 562 588 C92516 459 464 469 476 479 492 496 505 530 564 C92517 587 599 605 615 628 644 656 694 731 785 C92518 530 534 553 562 586 595 601 641 668 706 C92519 461 465 476 488 490 500 507 523 547 570 C92520 492 508 516 526 533 545 549 568 589 616 C92521 476 481 491 502 C92522 484 490 495 508 506 519 519 531 548 581 C92523 477 496 503 520 521 539 545 576 619 655 C92525 508 523 540 554 560 581 592 613 645 670 C92527 503 517 528 534 535 556 556 592 609 645 C92528 432 447 454 464 C92530 496 507 521 529 535 547 561 585 608 646 C92531 474 480 486 501 512 524 532 555 588 628 C92532 581 594 600 620 C92533 573 589 592 612 611 627 643 658 675 719 C92534 473 482 484 489 500 509 516 531 559 601 C92535 503 514 512 519 524 547 559 578 603 642 C92536 480 491 497 515 526 541 548 581 610 661 1283 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92537 594 612 632 647 654 665 685 724 767 808 C92538 506 521 534 551 571 581 594 631 668 733 C92539 566 582 591 607 620 635 643 685 721 764 C92540 523 536 545 557 562 576 583 613 662 694 C92541 566 583 593 602 610 622 640 667 701 742 C92542 508 512 509 517 518 526 534 559 584 607 C92543 500 505 509 517 531 542 539 558 579 606 C92544 420 468 495 505 521 529 538 560 589 616 C92545 482 497 502 513 518 538 546 575 609 639 C92547 591 606 614 632 632 648 644 668 689 716 C92548 465 469 480 489 498 512 510 537 575 630 C92549 438 441 445 449 458 463 468 479 509 538 C92550 521 535 548 559 571 591 602 644 686 730 C92551 455 458 471 475 480 496 499 527 542 556 C92552 507 516 524 541 553 567 573 600 631 687 C92553 362 365 372 371 376 376 383 378 375 368 C92554 449 457 461 465 470 484 487 511 534 565 C92555 475 489 502 526 536 553 562 610 661 700 C92556 489 496 508 517 524 544 553 576 610 662 C92557 483 494 502 514 520 536 536 562 598 623 C92558 555 557 562 576 582 593 597 626 646 696 C92559 484 489 490 500 505 517 524 539 568 599 C92560 492 506 512 524 526 541 546 572 602 655 C92561 500 512 527 534 544 553 555 588 618 650 C92562 483 493 499 518 512 528 536 560 588 619 C92563 514 522 534 540 542 561 572 594 618 656 C92564 524 537 561 576 589 606 620 662 705 760 C92565 498 517 521 531 534 542 550 571 620 672 C92566 534 544 553 566 559 576 577 552 C92567 510 514 516 534 538 551 548 583 611 637 C92568 489 503 514 515 524 535 548 567 588 627 1284 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92569 511 507 515 520 528 541 559 572 596 629 C92570 506 527 531 543 551 561 573 598 619 638 GROUP: MALE 2 - 0.5 PPM C92571 503 522 529 548 558 570 570 602 619 646 C92572 500 511 514 537 543 552 557 596 618 663 C92573 580 592 601 620 629 642 652 694 723 777 C92576 528 540 539 558 564 577 585 620 631 680 C92577 538 553 563 585 598 615 631 683 722 752 C92578 509 524 526 549 562 565 575 615 650 695 C92579 565 583 583 599 603 615 624 656 680 714 C92580 513 525 524 540 542 554 562 598 618 653 C92581 550 557 560 572 583 596 593 622 648 685 C92582 432 445 443 452 456 462 463 471 502 542 C92583 459 467 469 480 477 486 491 519 533 562 C92584 408 419 422 435 436 444 448 465 478 503 C92585 522 535 531 553 558 573 578 622 646 682 C92586 491 499 512 533 551 568 575 629 660 698 C92587 553 566 573 596 610 626 634 667 708 755 C92588 483 490 499 509 519 525 528 548 568 595 C92589 536 547 555 570 577 586 591 616 634 685 C92590 556 563 567 592 602 614 619 651 673 698 C92591 459 470 475 494 498 509 520 556 581 621 C92592 517 525 520 531 538 545 551 579 611 654 C92593 488 495 498 506 C92594 495 497 498 505 513 520 532 559 581 614 C92595 482 490 487 495 501 517 521 533 547 587 C92596 488 496 503 521 524 536 542 567 602 648 C92597 451 454 459 474 478 487 497 520 540 571 C92598 497 509 513 529 525 534 537 568 591 616 1285 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92599 559 569 568 587 592 605 603 642 663 693 C92600 471 480 487 499 C92601 471 471 478 495 500 510 524 551 583 606 C92602 434 437 433 445 445 460 461 491 508 517 C92603 513 519 532 543 545 557 555 C92604 510 518 532 550 557 575 580 627 659 698 C92605 528 541 551 570 576 592 608 647 695 735 C92606 483 495 504 519 529 540 546 592 621 643 C92607 528 541 552 571 576 588 600 626 660 673 C92608 557 574 587 604 579 576 578 623 667 710 C92609 487 490 493 504 509 518 528 562 600 629 C92611 474 483 487 497 505 510 512 540 563 580 C92612 506 516 523 538 534 551 562 615 586 670 C92614 537 539 548 563 574 582 587 617 654 675 C92615 498 507 520 539 548 565 566 612 658 696 C92616 406 411 416 428 C92617 570 583 601 614 630 644 654 704 741 766 C92619 453 460 466 483 482 495 496 527 537 559 C92620 424 429 433 449 454 459 468 496 514 538 C92621 512 530 539 555 C92622 504 515 528 538 543 555 564 592 634 662 C92623 434 447 458 468 474 482 494 524 543 578 C92624 468 479 487 511 513 514 529 567 610 650 C92625 567 578 595 610 616 637 647 689 728 756 C92626 529 539 548 561 572 560 566 598 639 645 C92627 529 545 554 560 C92628 536 547 548 564 573 586 589 613 653 674 C92629 518 528 543 560 571 585 593 649 680 738 C92630 520 531 535 555 559 571 580 623 654 672 1286 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92631 455 473 490 494 505 510 515 536 568 584 C92632 583 573 616 627 644 641 663 703 742 791 C92633 458 477 492 503 508 517 528 561 602 624 C92634 435 448 462 472 485 497 496 522 569 600 C92635 496 509 514 525 534 542 550 581 621 657 C92636 469 485 503 514 517 533 545 581 614 639 C92637 404 414 430 432 441 450 452 477 498 521 C92638 572 598 611 622 639 648 661 717 775 842 C92639 469 488 499 512 525 531 540 562 609 649 C92640 409 409 414 416 C92641 496 477 502 525 544 561 580 606 649 664 C92642 494 510 531 541 547 560 566 583 615 654 C92643 435 450 467 472 480 483 495 507 533 554 C92644 420 441 451 458 459 468 480 491 514 537 C92645 469 480 499 510 C92647 461 468 481 488 494 509 519 538 568 590 C92648 413 416 429 438 447 458 466 486 513 542 C92649 403 414 427 434 440 451 457 484 511 533 C92651 506 523 538 549 548 561 575 588 626 665 C92653 501 512 532 541 560 568 579 617 654 696 C92654 477 500 516 525 531 546 548 552 581 616 C92655 530 554 569 575 587 606 607 634 667 693 C92656 491 536 561 575 580 593 601 625 659 691 C92657 485 506 514 520 530 545 551 586 615 639 C92658 501 521 537 553 560 577 581 623 640 671 C92659 509 529 548 562 570 585 589 627 665 694 C92660 451 468 473 480 483 488 492 506 527 534 C92661 464 477 486 497 508 528 534 561 597 627 C92662 438 444 448 459 C92663 521 526 542 552 560 577 579 615 665 711 C92664 495 515 533 538 540 550 558 592 621 646 1287 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 31 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92665 543 555 568 578 590 606 614 639 680 721 C92666 509 531 551 557 563 571 581 590 620 653 C92667 530 556 577 592 604 614 620 666 694 704 C92669 452 471 489 497 505 515 529 567 592 628 C92670 432 449 461 463 469 474 482 507 516 538 C92671 462 477 487 499 512 522 527 559 581 608 C92672 454 478 496 508 515 528 539 568 602 628 C92673 501 516 524 537 540 552 559 594 618 634 C92674 470 485 498 513 526 544 551 597 633 663 C92675 460 479 479 487 496 506 516 539 558 577 C92676 453 463 477 482 C92677 461 469 479 473 486 491 501 542 552 582 C92679 499 510 526 539 546 560 575 618 640 672 C92680 533 553 568 576 577 589 596 642 652 676 C92681 473 492 506 516 524 533 547 570 600 629 C92682 465 479 490 499 507 518 533 570 594 626 C92683 492 507 519 526 537 544 552 593 622 662 C92684 436 450 454 457 C92685 508 517 534 538 555 570 579 619 652 683 C92686 488 494 507 517 524 527 539 576 600 630 C92687 522 552 567 581 596 607 619 658 685 715 C92688 457 467 480 488 492 499 501 530 543 563 C92689 457 469 468 478 491 503 511 545 576 595 C92690 603 612 630 642 652 668 677 727 736 773 GROUP: MALE 4 - 5 PPM C92691 472 487 486 496 506 526 532 566 595 620 C92692 487 512 517 529 534 547 547 581 592 622 C92693 498 513 514 526 541 554 561 581 603 633 C92694 517 530 529 532 546 560 572 603 616 651 1288 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 32 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92695 494 504 506 513 521 533 537 570 582 603 C92696 494 508 513 517 530 549 551 563 567 575 C92697 495 507 518 532 540 555 559 575 592 611 C92698 522 534 546 566 579 597 600 643 665 688 C92699 428 440 450 463 470 485 491 483 521 530 C92700 394 399 398 412 413 426 428 443 448 460 C92701 482 506 516 534 546 549 555 589 607 642 C92702 473 478 484 491 499 510 518 538 555 568 C92703 513 528 538 546 561 574 589 625 657 678 C92704 508 525 533 546 556 571 583 605 625 666 C92705 477 493 499 511 524 541 542 570 592 624 C92706 500 518 523 540 552 566 571 594 630 670 C92707 449 467 469 471 475 489 498 523 536 556 C92708 462 478 482 496 503 513 520 541 557 566 C92709 466 480 478 489 496 515 525 554 571 593 C92710 497 509 516 527 545 559 563 584 600 613 C92711 492 501 509 520 519 537 549 578 598 624 C92712 533 549 548 561 578 595 602 641 679 719 C92713 545 554 563 576 C92714 526 542 551 567 C92716 569 570 580 595 601 622 633 667 682 711 C92717 493 501 517 534 545 559 567 600 624 659 C92719 459 467 474 491 C92720 532 548 549 562 577 591 599 626 641 673 C92721 438 447 457 467 480 489 492 513 528 554 C92722 473 504 518 536 C92723 526 544 558 572 583 597 603 634 663 687 C92724 538 549 561 581 603 614 625 655 681 718 C92725 449 456 460 469 478 484 488 528 541 559 C92726 498 512 517 525 528 539 552 558 574 605 C92727 484 490 498 509 515 527 542 577 620 648 1289 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 33 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92728 567 587 596 611 C92729 554 566 581 596 621 635 641 683 716 764 C92730 467 483 483 491 505 523 530 553 591 628 C92732 477 486 495 507 525 533 546 572 599 639 C92733 442 448 446 458 473 486 492 513 537 574 C92735 507 513 521 531 541 554 565 575 598 622 C92736 470 477 488 504 518 537 545 557 587 629 C92737 481 490 494 505 521 537 543 565 595 630 C92738 471 492 495 508 520 529 532 545 560 591 C92739 424 436 439 447 460 472 476 488 521 545 C92740 504 509 521 529 543 553 566 578 597 625 C92741 499 511 508 527 533 546 560 578 600 618 C92742 567 587 604 623 639 655 663 680 713 751 C92743 545 565 575 591 612 631 638 672 706 741 C92745 536 557 567 590 605 624 636 678 721 756 C92746 548 575 578 592 596 613 622 646 665 706 C92747 479 488 498 511 522 543 545 551 570 602 C92748 530 544 558 570 569 585 604 615 638 664 C92749 440 450 458 465 486 504 510 522 544 573 C92750 484 495 499 513 527 546 552 547 594 629 GROUP: MALE 5 - 20 PPM C92751 506 515 524 539 547 564 577 613 647 681 C92753 496 499 501 514 514 529 535 567 559 556 C92754 420 426 431 441 440 448 449 459 467 499 C92755 449 461 463 479 491 504 509 538 569 595 C92756 531 541 552 573 584 602 603 630 648 679 C92757 431 426 432 447 451 465 467 489 514 538 C92758 464 477 485 500 506 520 518 542 559 576 C92760 486 494 500 513 522 528 527 546 554 582 1290 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 34 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92761 453 454 459 471 481 486 482 498 504 516 C92762 481 486 492 506 509 518 520 533 553 578 C92763 548 565 575 589 612 624 629 676 724 777 C92764 515 527 534 546 556 558 565 582 599 621 C92765 498 512 516 526 C92766 453 461 461 477 486 497 494 516 545 575 C92767 545 569 578 600 599 622 637 680 733 799 C92768 535 552 548 562 568 581 576 609 624 653 C92769 504 517 528 545 553 569 564 610 629 651 C92770 533 554 563 570 578 584 583 598 616 649 C92771 495 506 514 528 545 554 547 582 610 653 C92772 483 496 500 513 522 533 540 563 588 610 C92773 435 451 456 457 455 462 455 463 467 491 C92774 488 498 510 526 533 541 543 579 604 616 C92775 509 526 542 549 561 569 579 598 630 668 C92776 501 522 530 545 559 568 562 581 599 624 C92777 424 439 446 455 C92778 505 521 530 537 549 557 560 580 595 623 C92780 413 420 428 439 451 465 462 471 498 515 C92781 461 469 473 484 495 506 504 517 537 563 C92782 458 467 476 490 500 515 516 540 557 597 C92783 527 537 544 559 569 580 589 614 635 680 C92784 463 475 489 497 503 514 523 531 556 587 C92785 469 479 483 495 507 517 524 520 537 561 C92786 470 483 494 503 502 518 523 557 579 613 C92787 482 488 499 509 517 526 528 551 572 590 C92788 515 525 539 552 561 570 565 572 589 609 C92789 459 466 479 449 C92790 480 491 497 518 528 541 535 543 563 582 C92791 444 451 458 470 481 484 493 503 525 546 C92792 498 498 509 526 529 538 544 560 591 604 1291 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 35 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92794 444 456 460 474 479 488 489 505 531 549 C92795 462 472 480 489 502 515 521 543 571 599 C92796 427 430 437 448 453 462 466 480 499 519 C92797 453 465 479 487 493 505 512 537 561 590 C92799 503 521 535 552 C92800 470 481 484 498 501 509 511 513 525 556 C92801 538 560 571 590 596 612 615 643 677 722 C92802 456 470 476 483 493 501 508 523 551 589 C92803 495 502 514 520 524 528 536 544 558 547 C92804 448 461 467 483 490 498 510 520 541 560 C92805 510 527 543 555 563 575 581 611 629 661 C92806 456 473 496 518 524 538 535 555 571 604 C92807 470 479 491 499 513 530 536 548 569 599 C92808 433 443 446 457 462 473 478 483 493 527 C92809 481 494 503 509 520 527 529 546 561 588 C92810 465 472 481 494 503 514 518 533 561 586 C92811 486 496 509 513 526 539 551 581 609 648 C92812 491 504 513 523 C92813 479 483 495 506 516 519 526 541 562 584 C92814 376 375 375 374 377 377 389 392 406 436 C92815 415 419 430 442 452 450 460 462 479 507 C92816 428 436 445 456 463 472 474 493 514 542 C92817 547 550 568 581 592 600 613 657 702 744 C92818 460 470 475 482 491 495 499 522 537 566 C92819 412 416 414 416 426 430 432 457 476 508 C92820 398 405 415 415 427 442 442 455 466 489 GROUP: MALE 6 - 20 PPM C92821 447 456 471 481 485 491 488 508 475 518 C92822 440 448 455 465 480 488 491 520 542 580 1292 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 36 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92823 469 481 487 499 510 522 531 544 550 576 C92824 487 498 510 519 533 539 543 561 584 621 C92825 551 557 566 583 597 611 610 664 705 752 C92826 441 450 463 475 485 488 498 507 539 569 C92827 459 465 475 492 498 501 507 524 547 581 C92828 504 518 513 516 527 533 532 563 583 605 C92829 521 528 545 552 570 580 583 611 645 680 C92830 437 442 447 464 476 487 493 492 512 541 C92831 494 506 521 512 524 528 538 577 606 629 C92832 430 441 450 462 478 480 476 502 520 535 C92833 508 520 529 542 557 570 577 606 642 677 C92834 473 484 488 498 510 513 517 544 565 592 C92835 433 444 443 454 459 477 481 515 534 565 C92836 500 514 526 533 542 547 544 564 580 609 C92837 520 522 535 545 546 556 555 584 610 641 C92838 462 469 476 491 506 511 510 534 559 586 C92839 602 619 634 656 663 670 685 732 775 811 C92840 423 432 438 453 462 471 474 496 516 538 C92841 434 444 450 443 459 468 468 484 497 516 C92842 517 530 540 550 561 559 564 581 601 624 C92843 528 532 538 542 545 549 558 587 613 648 C92844 379 384 385 390 391 398 405 419 431 450 C92845 442 454 457 467 476 483 486 497 521 546 C92846 496 513 531 541 538 551 563 582 592 637 C92847 550 566 582 593 605 622 622 641 672 700 C92848 388 402 414 428 438 441 445 464 485 506 C92849 482 488 498 510 521 524 530 555 580 618 C92850 438 436 436 444 442 448 452 474 477 499 C92851 483 488 495 507 512 525 535 546 568 602 C92852 442 449 451 460 462 464 472 479 502 528 C92853 449 462 463 473 477 487 490 517 541 564 1293 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 37 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92854 477 493 501 510 516 527 530 553 583 626 C92855 450 456 464 471 480 493 487 499 524 559 C92856 441 448 454 461 474 484 487 503 525 543 C92857 512 517 520 527 537 544 551 572 601 629 C92858 503 518 532 553 563 575 576 608 631 663 C92859 479 488 498 510 517 526 525 547 573 602 C92860 433 442 442 454 465 465 475 491 522 549 GROUP: FEMALE 1 - 0 PPM C92862 241 241 243 246 261 260 257 253 258 268 C92863 277 281 284 290 287 290 287 303 315 333 C92865 276 280 292 284 286 293 289 322 337 344 C92866 279 289 296 296 296 304 304 317 320 345 C92867 300 329 324 323 318 324 311 329 346 363 C92868 283 290 302 311 315 314 308 316 335 343 C92869 235 239 244 248 256 259 259 267 273 280 C92870 259 265 272 272 276 290 287 291 307 316 C92871 287 295 295 299 292 291 296 307 324 351 C92872 224 233 234 235 240 239 244 274 291 309 C92873 226 236 243 246 246 253 256 261 269 279 C92874 273 281 287 290 293 294 298 306 321 324 C92875 251 264 261 263 272 283 282 286 292 299 C92876 258 266 266 272 271 279 278 290 298 301 C92877 259 264 267 277 272 279 289 295 326 328 C92878 256 261 269 262 272 272 279 283 285 293 C92879 310 327 320 325 331 331 331 332 357 380 C92880 267 274 273 283 C92881 286 295 301 296 306 314 313 321 333 360 C92882 245 244 243 251 250 249 258 264 254 272 C92883 327 337 344 338 346 352 350 357 367 383 1294 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 38 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92884 265 264 281 275 267 273 276 286 287 307 C92885 296 304 307 313 317 324 316 330 350 359 C92886 255 260 260 266 269 265 275 283 288 312 C92887 277 287 289 296 C92888 315 332 343 357 356 370 370 369 399 405 C92889 254 261 260 260 264 266 264 278 284 296 C92890 323 330 329 338 342 343 343 350 367 377 C92891 256 262 259 273 281 279 275 285 298 306 C92892 348 361 366 370 369 371 375 382 409 426 C92893 250 248 250 257 260 260 259 259 265 281 C92894 255 264 272 277 281 281 279 295 304 315 C92895 279 288 289 297 295 296 295 306 322 324 C92896 273 280 285 298 303 301 308 328 352 370 C92897 243 247 250 260 260 263 261 274 284 290 C92898 290 296 300 306 C92899 346 362 374 366 366 375 386 399 413 414 C92900 310 322 299 331 329 330 329 345 363 369 C92901 263 270 275 280 279 288 289 296 306 318 C92902 368 374 383 398 417 420 408 418 447 470 C92903 351 353 378 378 C92904 255 265 262 273 265 273 261 275 273 278 C92905 256 267 267 280 C92906 252 254 257 262 264 265 267 275 289 296 C92907 258 277 278 284 292 289 299 296 316 322 C92908 288 292 303 315 313 314 319 321 356 369 C92909 269 281 287 289 291 297 297 299 313 321 C92910 305 316 319 325 333 339 324 306 330 347 C92911 241 253 260 255 263 269 266 282 281 293 C92912 294 308 317 318 322 335 351 353 366 356 C92913 281 288 292 292 294 296 299 298 310 314 C92915 271 287 297 297 302 310 304 307 323 338 1295 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 39 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92916 336 357 369 388 403 414 426 439 457 472 C92917 346 359 354 367 372 381 375 390 409 425 C92918 274 292 295 295 305 313 313 311 324 336 C92920 252 264 267 277 280 285 284 290 309 326 C92921 318 338 353 355 373 380 369 389 420 445 C92922 282 291 300 298 304 307 299 314 332 345 C92923 246 243 253 260 268 270 271 285 299 303 C92924 301 312 316 315 319 324 320 320 333 336 C92925 315 325 326 330 332 333 331 339 358 373 C92927 282 286 281 287 288 292 291 295 315 324 C92928 286 290 302 304 309 311 323 331 352 344 C92929 268 269 281 278 281 288 288 289 312 327 C92930 361 388 406 421 435 448 455 473 510 532 GROUP: FEMALE 2 - 0.5 PPM C92931 333 336 334 348 348 370 368 395 417 461 C92932 272 264 270 280 282 290 294 305 306 305 C92933 318 337 337 353 355 355 367 390 415 452 C92934 254 263 266 271 275 277 281 286 300 313 C92935 304 307 311 321 315 320 327 341 359 374 C92936 266 263 270 273 275 274 285 288 286 307 C92937 279 279 285 291 293 296 304 318 330 342 C92938 298 310 309 307 311 323 322 341 354 358 C92939 245 239 246 244 247 254 257 272 274 295 C92940 259 256 265 270 268 272 278 286 295 302 C92942 314 314 317 325 334 335 334 338 347 354 C92943 320 315 319 324 330 343 344 348 345 361 C92944 365 380 369 380 C92946 259 249 260 267 259 264 268 274 292 301 C92947 300 302 305 311 300 307 306 324 326 342 1296 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 40 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92948 322 327 329 338 327 337 340 360 373 387 C92949 388 383 395 415 421 438 437 449 461 485 C92951 258 250 243 256 264 271 265 276 274 273 C92952 269 278 276 283 291 294 298 315 326 336 C92953 262 264 261 270 272 273 273 286 288 294 C92954 281 277 285 288 292 293 294 301 305 308 C92955 297 308 309 319 312 324 329 343 378 397 C92956 317 324 326 332 324 337 341 357 374 384 C92957 267 272 272 274 282 288 284 297 307 323 C92958 245 241 248 255 251 254 262 272 283 288 C92959 313 313 319 332 333 336 341 337 358 363 C92960 304 301 314 316 323 326 325 331 361 375 C92961 366 376 386 399 408 418 419 432 461 473 C92962 261 263 269 274 C92963 249 244 250 262 263 265 267 280 278 284 C92964 271 275 279 283 286 300 292 308 329 346 C92965 275 270 280 284 285 290 291 297 309 304 C92966 249 250 250 257 259 259 261 269 274 276 C92967 299 297 303 310 C92968 317 313 318 328 337 343 340 353 370 390 C92970 334 325 336 347 354 355 359 361 381 387 C92971 246 249 249 248 260 251 257 259 263 268 C92972 287 279 287 297 297 304 312 332 335 350 C92973 259 263 259 271 282 286 287 288 313 329 C92974 253 261 265 267 269 277 283 290 294 296 C92975 271 277 280 288 287 291 295 305 313 331 C92976 326 326 331 333 341 347 341 350 371 393 C92977 295 295 301 313 317 322 323 330 348 349 C92978 349 347 363 366 C92979 277 281 281 289 299 304 302 300 317 330 C92980 290 303 311 312 328 336 329 343 379 379 1297 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 41 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92981 300 292 307 303 316 323 313 331 361 349 C92983 313 301 310 325 326 329 324 333 338 354 C92984 317 320 325 333 330 337 337 349 364 374 C92985 316 313 322 338 330 343 342 356 358 375 C92986 267 269 272 272 267 279 279 288 293 309 C92987 287 288 286 287 C92988 281 283 280 283 298 309 303 318 331 353 C92989 273 278 279 284 290 290 288 311 326 339 C92990 282 285 287 283 292 299 296 296 303 325 GROUP: FEMALE 3 - 2 PPM C92991 250 250 259 261 265 267 273 278 284 305 C92992 265 272 272 273 275 286 282 301 318 331 C92993 289 292 291 302 C92994 272 277 278 285 273 287 285 302 301 304 C92997 253 255 255 269 264 267 273 289 289 296 C92998 290 295 304 309 309 307 318 324 339 367 C92999 302 295 299 308 299 314 315 335 342 356 C93000 285 286 290 293 C93001 256 261 268 271 274 280 279 298 316 347 C93002 267 259 263 280 277 276 288 302 307 317 C93003 305 300 315 324 327 333 337 329 343 361 C93004 273 274 283 287 288 298 294 310 321 326 C93005 257 255 265 275 282 291 289 313 316 341 C93007 267 264 273 277 272 275 280 291 303 320 C93009 265 275 288 289 294 290 300 301 333 320 C93010 277 280 289 295 292 301 308 314 318 338 C93011 294 299 299 309 311 311 323 321 337 341 C93012 316 321 325 328 330 337 347 365 376 397 C93013 240 246 250 256 261 263 269 273 281 285 1298 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 42 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93014 317 308 325 325 328 336 331 359 354 381 C93015 301 295 306 307 311 316 318 324 335 340 C93016 228 213 231 239 235 242 246 249 268 268 C93017 269 272 277 292 304 308 308 314 328 349 C93018 286 290 296 299 C93019 301 305 317 321 313 324 335 373 388 405 C93020 282 284 290 294 309 309 308 325 353 336 C93021 287 299 295 300 304 308 317 336 363 377 C93022 308 306 307 315 315 319 321 333 342 367 C93023 246 250 256 257 C93024 274 279 289 294 287 297 302 305 314 320 C93025 324 323 339 359 360 379 354 389 353 411 C93026 279 279 283 291 285 287 289 294 299 313 C93027 276 273 279 285 296 298 297 299 311 315 C93028 272 276 273 276 282 295 291 299 311 321 C93029 249 257 255 264 267 268 273 278 285 298 C93030 269 265 283 293 288 291 301 310 322 342 C93032 271 264 279 283 281 289 297 315 326 339 C93033 277 279 279 294 303 309 319 332 352 374 C93034 290 291 289 301 303 303 305 303 317 325 C93035 292 292 297 304 C93036 280 278 284 297 305 307 305 330 359 388 C93037 274 279 288 299 301 316 333 316 300 317 C93038 239 241 247 257 257 256 268 271 279 293 C93039 291 303 304 312 316 319 316 325 330 350 C93040 229 229 239 246 250 248 255 260 266 276 C93041 283 288 285 306 305 302 312 338 351 379 C93042 297 293 303 316 324 318 316 343 359 393 C93043 283 276 292 303 314 317 324 334 347 364 C93044 255 256 265 267 264 265 281 294 304 314 C93046 318 304 315 317 324 325 333 344 335 350 1299 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 43 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93047 331 330 346 348 357 367 373 373 401 410 C93048 238 238 239 250 249 247 257 259 270 282 C93049 258 260 261 268 276 273 277 289 295 301 C93050 280 274 281 297 309 305 303 309 311 319 GROUP: FEMALE 4 - 5 PPM C93051 261 260 255 260 C93052 279 279 284 286 302 297 308 316 335 340 C93053 260 274 272 283 290 285 305 319 361 381 C93054 260 258 263 264 271 267 284 287 293 303 C93055 288 286 293 303 306 307 307 318 332 346 C93056 284 276 279 291 294 298 299 302 313 332 C93057 254 254 259 270 275 279 283 274 296 309 C93059 280 278 283 293 292 297 299 300 315 337 C93060 275 285 294 299 302 307 310 304 320 318 C93061 281 281 279 290 300 300 306 322 354 379 C93062 265 262 267 275 278 287 280 291 296 311 C93063 272 274 272 288 285 298 299 302 308 324 C93064 252 255 257 266 C93065 223 221 228 232 238 235 240 249 260 269 C93066 267 266 275 282 278 289 278 298 302 342 C93068 274 275 278 285 289 294 295 305 326 332 C93069 328 329 336 346 352 356 358 369 400 417 C93071 244 239 248 249 C93072 272 272 278 283 288 293 289 308 314 333 C93073 278 273 279 288 295 303 302 310 315 321 C93074 298 298 304 313 314 316 322 345 368 391 C93076 279 290 296 296 297 299 303 319 336 356 C93077 296 299 305 315 314 319 321 339 364 379 C93078 270 266 268 278 275 283 279 296 305 311 1300 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 44 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93079 305 291 307 308 320 332 323 345 349 368 C93080 264 269 281 282 292 292 294 298 306 320 C93081 259 254 267 271 271 276 280 285 304 306 C93082 294 292 294 304 302 306 318 319 350 378 C93083 284 279 280 285 298 300 303 307 326 336 C93085 269 277 285 290 C93086 287 295 299 309 314 314 317 324 329 333 C93087 238 243 252 253 252 260 261 269 276 283 C93088 261 253 273 275 288 282 294 297 306 318 C93089 292 300 308 305 308 320 314 327 329 335 C93090 253 257 262 267 262 281 275 279 294 295 C93091 297 298 305 305 319 320 321 330 352 368 C93092 254 263 267 268 265 273 276 288 306 318 C93093 326 323 333 350 331 347 347 353 374 380 C93094 273 274 279 287 287 304 308 287 299 309 C93095 260 261 270 283 286 291 288 291 310 317 C93096 306 311 318 324 341 341 347 364 406 441 C93097 261 263 271 276 275 280 286 292 308 327 C93098 256 261 272 269 279 284 281 287 302 313 C93099 269 276 287 297 298 304 310 298 318 349 C93100 280 279 290 295 295 291 305 297 324 330 C93101 257 264 265 260 268 270 271 275 288 296 C93102 301 306 315 323 324 333 335 347 359 363 C93103 280 285 288 305 309 311 314 325 338 331 C93104 360 367 380 392 400 405 411 423 449 464 C93105 291 289 302 300 305 312 309 312 325 329 C93106 253 262 264 263 266 278 278 279 291 305 C93107 274 276 282 290 290 293 296 302 309 323 C93108 264 265 268 278 274 283 283 279 287 304 C93109 267 274 278 287 C93110 364 386 403 425 438 446 459 479 496 538 1301 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 45 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93111 311 300 305 312 C93112 271 275 278 279 284 283 285 299 309 325 C93113 229 240 242 237 241 251 248 253 262 267 C93115 224 223 223 228 235 233 234 243 253 262 C93116 248 242 250 254 256 254 263 267 273 280 C93117 258 259 265 261 271 266 279 290 296 305 C93118 236 232 234 246 250 254 261 275 293 276 C93119 220 216 220 224 223 233 232 245 253 251 C93120 244 235 244 244 240 251 249 256 267 278 C93121 218 219 222 218 223 226 227 235 246 250 C93122 293 295 303 299 305 305 311 311 329 344 C93124 246 258 271 288 270 264 264 276 272 286 C93125 216 220 221 218 227 232 232 237 244 254 C93126 226 229 233 231 239 243 242 256 266 285 C93127 255 251 253 258 C93128 250 249 258 266 268 266 272 276 289 295 C93129 248 265 256 266 271 259 271 306 282 287 C93130 268 274 281 281 290 289 292 298 317 319 C93131 278 277 284 291 287 293 302 309 320 327 C93132 258 268 264 268 269 277 274 298 315 332 C93133 238 242 245 241 250 249 253 262 275 267 C93134 281 286 291 285 287 296 297 311 318 328 C93135 273 269 273 275 275 284 288 297 275 289 C93136 248 243 259 265 262 269 280 275 292 301 C93137 240 236 245 242 249 252 255 264 271 284 C93138 269 261 271 271 275 280 290 292 304 308 C93139 224 226 232 238 236 240 245 262 264 272 C93140 271 275 276 282 285 298 307 324 343 367 C93141 290 286 286 292 294 298 300 316 324 341 C93143 291 277 287 293 C93145 230 231 226 235 235 232 240 248 255 263 1302 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 46 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93146 236 231 226 240 238 238 244 245 248 244 C93147 258 250 251 260 259 261 266 268 274 283 C93148 294 290 289 301 303 306 307 324 350 356 C93149 256 253 258 258 266 269 267 280 288 286 C93150 278 276 295 295 283 290 289 295 302 308 C93151 262 260 260 266 268 270 268 275 281 289 C93152 270 271 273 277 278 277 278 282 290 297 C93153 248 245 252 252 255 252 255 264 271 270 C93154 269 266 262 266 266 274 274 278 282 286 C93155 249 249 247 243 C93156 245 243 244 250 248 250 255 259 266 277 C93157 256 259 264 265 263 266 270 273 279 289 C93158 269 271 272 278 275 274 281 292 298 314 C93160 308 299 306 318 324 330 330 334 346 366 C93161 268 273 275 287 278 290 290 294 306 326 C93162 287 278 280 275 280 277 291 298 312 318 C93163 244 247 248 252 253 261 258 260 274 288 C93164 245 244 239 244 247 248 255 256 262 271 C93165 255 250 255 260 261 262 261 277 281 292 C93166 310 302 313 316 315 318 326 325 358 347 C93167 284 272 288 296 288 305 302 318 349 359 C93168 248 243 239 247 253 245 256 266 271 279 C93169 247 248 249 257 C93170 273 269 267 276 277 283 286 285 305 317 C93171 266 267 273 276 276 279 282 291 292 318 C93172 265 267 268 275 279 280 280 282 295 317 C93173 258 257 262 272 264 271 274 280 291 290 C93174 249 256 253 259 270 267 268 279 289 297 C93175 253 246 250 255 258 255 260 259 275 283 C93176 249 250 254 259 267 266 279 288 307 323 C93177 240 240 242 249 247 245 247 259 266 269 1303 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 47 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93178 251 253 250 255 252 258 257 267 271 283 C93179 244 241 241 250 248 251 250 259 264 265 C93180 298 293 297 299 299 305 311 315 327 337 GROUP: FEMALE 6 - 20 PPM C93181 252 255 255 259 267 268 265 272 284 291 C93182 245 247 245 257 261 268 257 268 269 277 C93183 287 290 295 297 303 309 312 313 328 356 C93184 233 238 235 241 244 239 244 242 253 260 C93185 260 265 265 273 280 282 278 287 299 286 C93186 224 226 228 221 232 228 236 239 251 242 C93187 256 269 267 270 280 282 292 318 345 358 C93188 267 272 281 290 290 292 295 302 318 331 C93189 237 242 242 243 249 246 242 249 259 259 C93190 263 265 274 274 275 280 280 289 300 315 C93191 319 309 310 316 317 311 319 323 342 343 C93192 236 234 241 247 246 248 247 262 267 282 C93193 249 256 260 262 274 266 278 294 314 341 C93194 293 300 306 308 308 316 315 320 327 334 C93195 272 278 280 285 288 290 291 291 305 315 C93196 226 231 229 235 238 240 244 250 260 269 C93197 222 225 232 237 234 233 238 236 239 245 C93198 251 257 266 271 273 278 277 280 292 301 C93199 250 255 260 262 263 266 267 271 275 286 C93200 238 245 245 255 256 257 255 261 270 280 C93201 238 236 234 243 247 249 249 257 261 271 C93202 247 244 248 254 253 251 242 258 263 268 C93203 264 275 270 276 278 282 292 313 324 339 C93204 249 255 253 255 258 267 268 273 282 293 C93205 283 291 295 295 296 302 300 305 309 308 1304 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 48 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 17 21 25 29 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93206 230 232 239 241 239 250 245 263 272 289 C93207 234 233 243 247 247 249 256 261 269 277 C93208 240 244 250 256 256 260 265 264 274 277 C93209 247 247 249 254 254 257 260 262 275 285 C93210 268 266 271 285 281 287 293 295 310 319 C93211 234 232 238 238 243 248 248 241 244 251 C93212 272 285 282 280 284 286 292 300 315 326 C93213 253 265 259 269 268 272 272 278 288 303 C93214 240 246 248 253 254 261 267 269 285 295 C93215 263 268 275 276 275 282 282 289 301 315 C93216 249 252 261 263 260 264 270 273 295 313 C93217 291 297 304 306 313 316 312 323 340 344 C93218 233 237 239 246 246 252 247 259 268 278 C93219 301 302 293 309 310 309 313 312 327 341 C93220 278 284 299 303 308 314 315 334 344 359 1305 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 49 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92501 796 822 812 835 855 890 C92502 578 593 596 603 617 631 646 647 653 668 C92503 655 674 695 713 729 673 700 759 766 780 C92504 591 607 621 633 673 690 C92505 551 580 609 617 632 649 666 686 697 711 C92507 618 621 631 647 659 675 C92508 589 607 600 613 617 651 669 683 680 680 C92510 660 685 688 716 711 713 731 726 735 746 C92512 635 662 676 691 693 684 C92513 642 662 655 683 684 694 713 718 710 727 C92514 821 842 846 854 836 866 868 875 912 949 C92515 609 641 649 577 641 674 696 715 729 737 C92516 606 622 625 644 C92517 821 854 870 897 922 938 C92518 734 750 759 783 790 816 835 875 877 884 C92519 592 610 618 631 638 650 665 691 688 700 C92520 637 668 664 693 706 711 710 720 708 684 C92522 616 644 645 678 693 720 750 766 787 804 C92523 693 722 718 757 777 805 C92525 695 727 741 764 776 795 812 679 550 506 C92527 674 697 706 731 757 767 779 772 803 819 C92530 675 693 700 715 734 739 731 758 765 775 C92531 648 671 662 670 665 671 669 659 665 C92533 727 765 777 806 820 846 865 873 890 901 C92534 627 647 640 642 666 665 C92535 671 698 690 617 692 729 750 766 794 811 C92536 681 701 703 738 746 774 792 805 837 815 C92537 857 891 905 937 961 966 984 1015 1024 1064 C92538 774 821 866 911 968 1008 1071 1109 1150 1192 C92539 795 822 816 835 846 884 901 924 941 950 C92540 719 755 779 807 827 855 C92541 753 765 792 812 830 849 864 893 897 920 1306 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 50 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92542 615 632 645 664 687 705 704 730 729 749 C92543 620 634 632 651 669 685 697 703 711 724 C92544 632 618 629 615 629 642 643 656 629 662 C92545 678 715 736 746 795 824 846 869 888 911 C92547 739 754 758 770 794 794 805 814 826 837 C92548 664 688 708 735 757 769 775 791 790 783 C92549 544 555 567 588 614 622 631 652 619 667 C92550 757 780 803 832 870 889 904 914 937 947 C92551 576 593 610 619 638 645 664 673 683 716 C92552 712 731 738 769 799 826 843 850 871 895 C92553 384 401 372 377 390 371 366 353 C92554 577 591 597 610 637 648 645 658 662 684 C92555 736 754 791 806 808 828 784 C92556 693 717 710 739 770 767 672 499 C92557 651 680 693 715 725 732 755 775 800 805 C92558 722 752 755 770 784 793 800 811 818 819 C92559 613 643 661 668 692 707 715 738 744 758 C92560 691 713 722 746 771 792 807 831 833 839 C92561 675 701 715 729 753 756 774 747 C92562 641 656 665 681 711 703 C92563 683 684 689 682 701 704 720 732 717 717 C92564 786 823 821 850 870 877 883 894 904 826 C92565 707 721 741 770 800 816 824 850 864 868 C92567 688 692 702 729 753 772 784 818 829 849 C92568 670 685 687 709 725 745 754 759 779 785 C92569 640 655 644 659 667 668 679 681 686 683 C92570 661 665 667 680 696 686 1307 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 51 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92571 689 695 706 722 767 786 800 833 852 869 C92572 696 716 734 749 774 772 801 827 832 854 C92573 814 837 823 858 892 910 929 946 956 850 C92576 721 748 771 797 819 828 868 887 901 920 C92577 811 838 851 882 907 917 936 963 966 965 C92578 720 748 779 804 837 856 888 911 938 965 C92579 757 780 788 804 813 822 842 851 838 842 C92580 687 706 716 740 767 780 798 829 849 872 C92581 719 739 758 787 808 829 849 873 907 934 C92582 574 586 590 587 617 629 627 642 658 656 C92583 587 599 622 641 665 700 718 724 739 744 C92584 539 551 550 558 582 594 576 C92585 697 575 C92586 749 795 833 865 892 921 944 911 912 748 C92587 777 797 803 813 836 851 836 844 850 856 C92588 617 638 635 653 667 683 674 672 685 685 C92589 707 729 729 757 790 810 824 826 827 826 C92590 712 731 748 764 790 801 812 816 833 845 C92591 652 677 688 703 726 745 753 761 782 801 C92592 699 723 736 759 789 807 813 837 859 875 C92594 632 654 665 670 694 712 720 721 736 756 C92595 607 626 622 631 657 684 691 701 703 695 C92596 678 699 706 719 733 750 756 752 729 575 C92597 591 605 604 614 629 647 657 664 667 686 C92598 645 660 665 679 701 707 713 728 746 745 C92599 721 749 758 787 815 816 C92601 639 661 674 700 726 736 756 758 768 789 C92602 533 563 563 565 600 602 599 599 600 604 C92604 739 768 795 820 843 867 877 913 942 950 C92605 765 787 812 824 854 848 863 867 887 886 C92606 671 696 714 733 758 766 768 769 764 757 1308 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 52 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92607 700 731 754 778 794 799 815 842 849 861 C92608 745 741 784 786 813 832 870 868 836 790 C92609 652 663 692 714 743 752 764 768 781 792 C92611 605 623 629 636 647 652 665 665 679 685 C92612 696 721 741 763 787 805 823 844 864 870 C92614 679 693 699 709 718 730 722 726 732 751 C92615 732 766 781 811 838 862 873 884 913 918 C92617 804 829 852 891 894 889 871 824 810 818 C92619 578 576 583 599 624 620 636 650 650 660 C92620 554 567 575 595 603 601 614 611 616 629 C92622 694 721 739 772 796 805 822 839 848 863 C92623 598 608 612 631 636 645 659 666 668 670 C92624 688 726 755 768 804 827 829 783 C92625 777 804 826 843 874 886 887 901 900 891 C92626 655 632 653 661 697 703 710 721 696 736 C92628 694 705 698 738 768 698 758 628 557 513 C92629 776 805 834 871 906 924 918 922 919 869 C92630 694 719 731 742 755 757 764 750 747 733 GROUP: MALE 3 - 2 PPM C92631 600 625 632 653 670 674 679 698 705 718 C92632 801 837 857 869 888 901 914 901 861 848 C92633 670 697 715 740 766 785 787 797 817 835 C92634 617 646 655 688 715 733 737 770 787 791 C92635 681 696 714 739 755 764 757 780 766 775 C92636 666 689 705 734 743 771 792 823 827 841 C92637 551 560 574 595 614 625 629 661 676 691 C92638 877 917 931 955 988 998 1003 1020 1007 1002 C92639 664 696 706 721 743 755 770 783 798 792 C92641 667 689 726 735 723 726 749 717 732 729 1309 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92642 685 697 705 721 737 751 751 764 763 772 C92643 574 581 587 599 616 624 623 636 638 656 C92644 555 577 578 589 608 625 632 660 671 678 C92647 626 644 654 673 700 708 714 739 748 767 C92648 565 591 598 619 624 640 648 669 685 689 C92649 547 559 568 585 598 610 617 635 644 664 C92651 691 719 730 744 780 799 818 844 841 805 C92653 739 767 795 820 841 864 842 879 889 909 C92654 637 666 667 665 680 682 689 699 710 726 C92655 721 736 758 775 795 804 812 821 832 850 C92656 706 738 742 756 777 789 799 815 836 843 C92657 664 694 700 705 721 733 742 753 759 760 C92658 708 727 744 762 781 804 815 837 846 860 C92659 718 735 751 772 784 792 800 823 817 821 C92660 552 567 570 586 596 603 601 606 603 615 C92661 648 638 642 654 686 703 715 714 721 735 C92663 749 779 795 825 843 879 886 924 924 935 C92664 672 704 707 714 740 756 760 758 760 769 C92665 756 775 794 811 827 844 856 891 915 908 C92666 679 705 717 738 771 780 799 805 823 745 C92667 733 755 786 802 817 821 819 856 864 894 C92669 658 678 705 717 739 755 759 783 804 812 C92670 553 570 584 593 598 594 582 569 530 481 C92671 622 664 674 675 697 685 705 727 734 759 C92672 660 685 713 728 755 782 800 825 852 863 C92673 656 676 687 713 728 737 761 784 814 832 C92674 704 723 747 771 783 809 822 850 864 876 C92675 596 615 630 655 667 683 701 721 732 750 C92677 612 635 648 666 681 685 705 728 734 753 C92679 709 748 765 811 849 845 C92680 704 720 731 736 760 778 795 802 799 646 1310 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 54 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92681 650 672 684 704 716 739 748 765 776 771 C92682 652 676 697 720 738 753 778 798 806 820 C92683 697 726 757 782 800 820 818 809 844 886 C92685 711 737 750 773 800 805 825 837 854 867 C92686 654 684 709 734 764 787 799 832 855 881 C92687 745 704 758 787 779 784 780 766 691 545 C92688 586 590 582 610 623 634 638 648 658 668 C92689 609 621 647 667 689 693 C92690 795 817 835 863 895 916 934 961 986 1009 GROUP: MALE 4 - 5 PPM C92691 641 671 675 700 707 720 721 748 758 772 C92692 642 660 680 673 632 C92693 654 674 679 704 721 733 731 754 770 777 C92694 675 700 720 739 758 778 778 801 807 838 C92695 611 629 648 664 675 686 690 692 708 720 C92696 598 616 618 651 678 684 685 703 728 728 C92697 626 645 657 676 703 709 721 743 643 C92698 718 735 760 794 829 856 890 921 944 977 C92699 555 576 583 593 586 593 597 606 631 634 C92700 471 493 507 533 546 553 563 570 579 590 C92701 659 670 688 709 730 560 C92702 590 607 619 633 643 655 671 686 699 701 C92703 684 711 739 760 785 811 804 686 C92704 685 715 723 743 755 773 779 808 823 843 C92705 650 683 702 726 748 766 771 772 793 807 C92706 712 742 785 825 861 878 900 933 978 1001 C92707 582 594 608 630 638 649 658 675 692 703 C92708 574 596 611 637 655 668 663 682 698 709 C92709 593 596 604 628 655 669 665 681 685 675 1311 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 55 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92710 616 637 652 670 692 702 703 712 711 720 C92711 645 667 679 701 721 745 746 760 787 800 C92712 739 761 779 816 842 863 878 877 894 905 C92716 733 734 673 647 629 618 566 531 566 545 C92717 684 710 720 738 762 779 782 802 821 828 C92720 690 721 739 770 791 816 824 832 858 884 C92721 570 587 603 590 598 621 624 651 657 673 C92723 701 717 729 746 769 767 791 820 830 837 C92724 740 767 784 822 851 871 878 907 937 954 C92725 576 596 609 613 610 C92726 596 611 623 647 666 679 687 695 717 723 C92727 667 700 714 747 780 796 790 813 845 845 C92729 785 812 850 879 903 917 937 960 888 790 C92730 649 677 674 690 714 740 747 769 778 797 C92732 647 689 707 738 755 773 774 796 812 820 C92733 567 582 612 628 644 656 666 682 690 695 C92735 652 676 685 704 717 732 741 759 772 770 C92736 663 700 719 737 769 788 793 821 835 854 C92737 653 686 698 715 728 753 767 788 798 805 C92738 599 624 633 649 659 665 682 695 714 733 C92739 562 568 585 598 613 625 630 657 670 682 C92740 651 661 668 681 697 714 731 746 750 769 C92741 636 650 663 682 697 718 711 725 738 745 C92742 789 813 834 844 862 886 889 906 904 922 C92743 780 829 859 883 905 931 965 984 1017 1026 C92745 820 867 904 941 967 1010 1029 1065 1104 1123 C92746 736 759 770 778 795 819 827 850 853 886 C92747 632 666 672 681 696 722 726 757 779 792 C92748 682 710 729 732 750 767 764 787 784 809 C92749 593 608 636 658 672 686 695 703 723 729 C92750 655 681 696 708 725 729 755 771 794 812 1312 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 56 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92751 710 722 764 791 818 843 C92753 581 616 643 644 617 640 663 682 698 719 C92754 518 542 553 558 573 588 C92755 620 651 671 689 699 732 C92756 701 739 761 774 800 815 834 836 863 865 C92757 557 566 587 597 603 617 634 656 668 683 C92758 589 612 635 641 630 576 510 423 C92760 604 628 648 663 685 707 730 754 765 713 C92761 532 552 572 578 594 604 617 603 619 641 C92762 597 629 649 678 712 727 760 789 799 816 C92763 830 886 919 962 992 1017 1044 1082 1080 1100 C92764 642 673 690 703 731 741 758 765 788 746 C92766 587 625 637 650 654 638 632 650 653 659 C92767 857 921 974 1026 1088 1114 1124 1196 1225 1246 C92768 669 706 722 731 766 777 789 791 808 817 C92769 665 670 696 706 724 743 764 780 799 815 C92770 687 724 758 783 826 846 877 904 923 916 C92771 673 716 734 756 770 799 824 859 876 905 C92772 620 648 665 666 684 C92773 496 515 539 546 559 560 565 564 549 557 C92774 652 664 683 695 708 730 742 745 744 757 C92775 697 728 765 751 800 813 831 843 863 878 C92776 642 659 678 686 700 708 729 736 750 755 C92778 648 680 683 701 726 724 C92780 525 550 561 583 596 619 631 640 640 654 C92781 587 607 626 639 660 671 682 705 718 708 C92782 613 645 681 708 735 755 766 802 831 862 C92783 701 745 769 796 813 846 878 910 926 938 C92784 615 644 677 698 729 749 778 784 806 814 C92785 582 611 630 646 663 678 702 719 731 752 C92786 630 663 684 696 716 726 752 769 785 790 1313 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 57 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92787 600 607 625 589 C92788 625 648 661 683 697 715 720 745 771 776 C92790 597 611 625 638 655 667 C92791 574 609 639 661 693 717 733 754 768 786 C92792 625 643 646 665 678 697 704 722 747 755 C92794 573 598 602 625 632 630 541 C92795 621 640 664 679 702 710 C92796 521 544 556 570 583 573 C92797 610 635 662 680 691 694 711 725 737 754 C92800 572 594 586 586 610 625 635 662 664 665 C92801 767 818 846 855 880 910 C92802 625 646 666 696 733 752 786 824 829 865 C92803 599 622 618 623 644 657 667 676 679 697 C92804 602 632 661 678 713 732 740 769 792 813 C92805 681 694 716 720 739 747 783 802 816 692 C92806 631 640 669 664 670 680 694 684 711 719 C92807 629 670 679 690 725 737 760 789 801 818 C92808 534 554 555 553 574 583 591 602 609 617 C92809 602 623 641 648 661 672 684 697 710 726 C92810 622 650 671 686 716 737 746 780 796 809 C92811 677 C92813 612 637 651 679 698 722 741 747 769 778 C92814 456 471 470 478 495 512 517 535 541 540 C92815 522 535 538 545 557 573 590 604 609 616 C92816 569 584 593 605 625 630 649 665 C92817 809 853 890 933 982 1008 C92818 591 621 626 632 659 672 685 696 712 714 C92819 537 562 578 583 617 633 654 669 687 700 C92820 504 516 520 497 530 537 1314 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 58 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92821 546 570 577 589 600 605 631 640 639 638 C92822 615 630 638 648 679 703 744 749 767 753 C92823 595 616 628 640 646 664 663 681 709 732 C92824 639 654 651 669 693 699 718 747 767 785 C92825 787 823 863 911 961 998 1036 1081 974 1038 C92826 600 630 641 655 683 703 C92827 601 625 636 648 672 683 748 744 756 767 C92828 634 655 669 671 696 714 745 764 790 796 C92829 704 734 740 770 789 800 817 849 870 902 C92830 574 576 572 593 608 591 587 605 628 629 C92831 639 644 637 636 660 682 699 701 701 700 C92832 555 571 571 585 602 610 630 649 651 636 C92833 715 758 782 814 856 882 918 957 983 992 C92834 620 631 661 668 690 702 726 742 755 766 C92835 591 608 621 631 644 647 660 672 691 698 C92836 635 656 681 706 727 742 771 660 785 821 C92837 673 699 716 730 751 772 794 722 464 C92838 613 628 646 660 685 697 717 745 644 C92839 866 917 966 1003 1043 1059 1107 1103 1118 1140 C92840 557 586 598 610 631 648 667 670 696 705 C92841 530 569 582 594 613 624 652 680 716 C92842 665 689 696 718 745 765 756 786 804 809 C92843 676 712 728 756 790 808 849 879 908 937 C92844 475 491 486 481 497 509 514 524 523 535 C92845 571 585 590 606 620 619 634 655 653 661 C92846 670 709 716 749 774 789 762 781 790 777 C92847 735 784 806 820 855 874 913 938 941 950 C92848 529 548 551 549 583 588 585 576 542 525 C92849 646 672 692 723 747 764 794 808 727 537 C92850 504 537 550 560 581 589 589 620 635 663 C92851 613 654 670 687 714 731 767 786 799 812 1315 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 59 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92852 557 582 593 613 641 650 680 685 691 C92853 597 615 632 648 674 676 717 752 773 798 C92854 647 679 693 716 743 759 783 820 847 880 C92855 573 594 618 619 631 664 685 646 557 481 C92856 565 572 581 594 618 625 653 661 661 678 C92857 646 677 702 714 744 785 809 736 805 862 C92858 684 696 701 717 748 765 794 811 837 834 C92859 613 630 638 661 680 690 703 683 617 544 C92860 562 570 572 586 585 608 622 646 663 670 GROUP: FEMALE 1 - 0 PPM C92862 269 278 283 304 315 310 C92863 369 369 396 389 400 434 452 474 482 498 C92865 361 381 397 408 421 428 444 461 C92866 356 340 358 377 408 413 421 438 451 456 C92867 380 384 405 414 433 440 467 485 481 501 C92868 360 376 389 407 420 423 442 444 449 456 C92869 290 293 300 312 345 328 357 366 374 386 C92870 338 346 364 380 389 410 422 440 453 482 C92871 367 371 384 397 416 435 452 474 506 531 C92872 336 348 353 377 392 401 422 435 434 459 C92873 289 290 313 328 339 352 362 378 381 403 C92874 355 377 401 426 437 453 467 481 488 506 C92875 316 322 350 369 385 392 412 414 398 422 C92876 326 330 347 370 377 384 C92877 357 382 393 417 433 438 454 481 492 493 C92878 308 311 328 349 363 374 401 401 419 441 C92879 399 424 441 462 475 483 485 512 511 537 C92881 361 374 388 414 424 431 447 459 467 467 C92882 288 300 311 341 353 367 375 398 413 418 1316 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 60 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92883 388 382 398 412 418 424 442 471 471 470 C92884 313 327 344 356 367 385 390 410 405 426 C92885 375 399 418 418 457 462 476 494 492 513 C92886 323 347 373 398 415 426 440 441 442 402 C92888 436 480 499 538 549 568 599 623 645 669 C92889 319 348 347 357 385 398 C92890 377 385 395 417 436 463 473 493 500 515 C92891 318 331 337 349 358 358 370 380 382 393 C92892 442 479 504 538 577 580 591 641 652 668 C92893 290 295 304 312 315 323 332 339 336 350 C92894 323 326 342 352 358 363 C92895 321 332 351 367 374 374 401 424 416 436 C92896 373 384 404 421 437 440 468 476 486 505 C92897 294 299 312 333 344 348 366 382 388 390 C92899 430 450 462 469 483 478 C92900 368 396 423 443 466 488 520 533 554 577 C92901 333 353 359 375 386 392 421 423 432 444 C92902 514 531 571 607 628 648 680 704 726 763 C92904 290 278 307 334 344 321 347 386 401 417 C92906 317 327 338 348 359 362 385 383 397 404 C92907 335 372 348 353 359 358 372 389 407 414 C92908 377 408 445 465 485 507 535 551 560 573 C92909 327 355 354 369 387 384 397 418 422 431 C92910 349 359 377 403 412 420 C92911 305 312 313 331 345 351 350 355 357 368 C92912 386 420 428 473 508 522 560 593 611 640 C92913 323 326 327 325 347 347 358 371 381 390 C92915 363 392 414 441 453 470 502 514 526 542 C92916 503 527 548 570 579 579 607 640 654 686 C92917 454 474 501 522 542 560 C92918 344 364 380 399 423 426 457 485 492 476 1317 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 61 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92920 330 340 348 371 375 383 404 413 421 442 C92921 486 514 542 554 477 350 306 C92922 370 398 426 462 477 487 C92923 319 340 361 381 393 403 C92924 347 365 372 396 417 422 449 472 478 485 C92925 395 417 432 464 487 492 524 543 572 596 C92927 334 338 354 366 372 371 387 394 391 401 C92928 356 387 425 456 477 491 525 546 569 594 C92929 360 347 364 391 347 304 294 C92930 559 593 617 646 666 668 GROUP: FEMALE 2 - 0.5 PPM C92931 499 519 564 596 607 640 667 715 755 758 C92932 330 316 267 C92933 484 508 519 519 531 545 554 581 591 591 C92934 310 336 363 370 382 413 432 446 438 439 C92935 415 427 463 479 496 518 550 579 603 619 C92936 320 336 352 362 368 377 387 389 398 414 C92937 356 374 393 408 420 431 370 388 283 C92938 364 397 408 431 463 493 522 552 584 615 C92939 300 321 339 351 360 374 389 378 379 410 C92940 306 310 326 347 354 372 398 399 429 437 C92942 360 370 389 427 438 C92943 404 427 458 472 489 503 533 540 550 585 C92946 335 352 380 401 408 421 423 426 447 480 C92947 340 347 368 383 385 411 421 425 436 440 C92948 408 431 451 473 485 510 552 562 570 586 C92949 472 C92951 282 290 323 327 317 325 356 372 387 401 C92952 344 363 386 398 411 415 429 438 357 1318 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 62 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92953 301 305 337 328 343 344 360 368 375 379 C92954 321 340 356 373 389 402 415 436 456 479 C92955 422 443 475 485 492 507 544 569 588 607 C92956 408 418 443 466 489 502 529 537 555 572 C92957 337 360 355 389 395 406 434 438 460 461 C92958 304 320 340 354 366 376 385 401 408 432 C92959 371 389 392 431 437 440 465 418 473 455 C92960 395 414 423 459 473 481 501 509 533 548 C92961 504 532 557 580 591 613 645 667 677 710 C92963 315 327 335 345 354 376 364 392 390 401 C92964 362 371 387 398 412 418 418 442 454 479 C92965 327 328 343 364 378 393 419 436 458 470 C92966 281 287 293 302 304 305 315 330 340 329 C92968 400 445 464 431 408 402 405 411 399 C92970 408 427 442 470 488 516 530 554 569 594 C92971 266 276 277 286 294 297 302 313 324 337 C92972 362 372 391 400 417 419 445 445 444 485 C92973 348 383 400 440 466 487 508 532 544 556 C92974 300 326 344 352 356 365 390 395 401 425 C92975 333 352 374 385 389 402 413 420 421 431 C92976 411 431 450 481 497 525 546 540 528 561 C92977 360 382 416 428 449 455 481 488 497 519 C92979 334 353 377 392 407 409 436 454 461 473 C92980 373 412 425 458 453 466 432 426 C92981 381 372 394 426 431 437 460 474 486 411 C92983 366 373 386 406 414 419 445 450 470 483 C92984 384 389 410 433 447 453 474 477 497 507 C92985 421 400 434 465 480 477 499 540 554 598 C92986 310 318 326 341 347 348 362 368 375 395 C92988 359 374 404 422 447 445 463 478 494 518 C92989 353 361 377 398 409 390 397 419 430 431 1319 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 63 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92990 338 367 386 407 428 436 450 475 497 511 GROUP: FEMALE 3 - 2 PPM C92991 320 338 337 357 355 383 395 421 428 466 C92992 348 361 385 410 420 439 474 460 480 497 C92994 315 325 336 336 349 364 379 371 375 391 C92997 302 307 313 323 329 326 342 348 356 369 C92998 383 391 299 216 C92999 377 388 415 434 449 458 470 483 493 528 C93001 363 383 393 402 415 422 450 455 460 480 C93002 336 343 365 384 390 405 427 441 454 496 C93003 383 390 415 434 432 447 462 478 475 494 C93004 343 365 392 422 441 450 485 504 521 471 C93005 364 364 378 381 396 401 436 439 436 466 C93007 323 339 345 368 383 389 415 435 441 454 C93009 365 372 402 396 413 431 445 457 461 472 C93010 377 392 414 422 431 444 481 492 510 527 C93011 356 382 402 417 432 452 469 490 509 518 C93012 407 416 439 458 462 488 503 513 512 540 C93013 308 319 334 353 368 382 412 437 457 478 C93014 410 430 453 482 505 528 568 604 628 590 C93015 357 365 363 393 400 407 426 449 431 431 C93016 289 302 304 326 337 334 350 369 324 326 C93017 367 373 388 408 423 440 463 483 479 502 C93019 425 458 475 298 C93020 355 392 410 405 442 452 493 511 402 C93021 392 402 429 453 477 476 413 338 C93022 403 405 439 452 472 478 509 546 575 595 C93024 330 350 367 394 403 427 444 475 483 491 C93025 386 428 434 457 452 476 505 545 562 576 1320 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 64 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93026 320 331 350 356 363 364 382 393 391 405 C93027 329 340 354 367 384 384 414 435 462 476 C93028 351 350 366 383 391 405 411 436 447 463 C93029 310 320 328 336 346 355 364 383 394 410 C93030 382 410 421 447 468 486 510 516 536 557 C93032 361 384 400 415 433 443 460 493 513 519 C93033 384 395 398 376 371 339 307 308 289 C93034 341 342 355 357 365 380 389 412 416 419 C93036 393 428 450 478 505 525 549 575 615 649 C93037 326 354 368 402 429 459 463 486 445 C93038 305 312 334 349 363 386 401 425 471 534 C93039 379 386 408 440 459 478 510 524 540 558 C93040 285 291 298 313 323 328 333 347 347 364 C93041 399 416 435 458 461 484 489 507 521 524 C93042 427 453 478 521 546 567 597 638 659 647 C93043 388 400 414 431 455 473 496 523 536 556 C93044 312 328 337 358 370 391 412 387 339 C93046 360 381 383 397 404 423 444 464 466 482 C93047 480 481 522 563 571 601 650 670 697 739 C93048 291 302 306 324 324 329 346 376 379 396 C93049 325 341 361 371 388 387 412 431 392 314 C93050 335 344 364 393 407 422 451 467 474 497 GROUP: FEMALE 4 - 5 PPM C93052 357 370 404 417 444 467 492 513 484 433 C93053 398 414 431 453 474 495 508 466 350 C93054 313 325 346 360 382 386 415 415 390 392 C93055 395 387 409 442 479 508 532 550 585 609 C93056 351 358 367 380 386 392 413 424 443 461 C93057 321 323 341 351 361 368 383 399 400 418 1321 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 65 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93059 342 370 372 392 407 413 438 461 473 489 C93060 332 332 350 376 374 391 415 439 424 466 C93061 404 410 427 453 470 493 522 528 534 555 C93062 313 328 337 349 356 365 378 393 393 406 C93063 333 349 358 377 388 397 408 422 412 435 C93065 274 289 298 308 319 333 350 366 371 375 C93066 365 367 391 404 419 443 454 484 463 463 C93068 338 351 365 377 385 396 420 424 419 435 C93069 426 432 434 456 463 484 503 511 522 534 C93072 373 373 382 401 418 416 434 444 454 464 C93073 331 340 338 349 371 405 422 442 431 393 C93074 416 432 453 410 472 506 553 578 586 607 C93076 359 386 401 429 430 428 470 495 504 557 C93077 387 398 413 434 440 450 427 445 444 483 C93078 327 340 350 361 368 381 423 441 441 465 C93079 370 385 393 440 445 486 515 526 540 578 C93080 344 352 365 380 390 408 424 427 433 445 C93081 319 328 341 350 367 391 428 436 429 458 C93082 400 404 412 429 436 437 446 463 466 489 C93083 340 353 368 387 397 427 442 454 462 493 C93086 348 354 369 382 387 408 436 446 466 487 C93087 295 303 294 323 327 335 360 372 369 388 C93088 342 342 349 360 369 387 404 412 412 376 C93089 330 339 356 363 372 380 396 407 397 419 C93090 301 302 314 344 341 356 372 399 408 421 C93091 368 390 400 420 432 449 472 501 516 549 C93092 326 337 347 356 358 367 393 406 397 429 C93093 389 409 432 445 463 473 492 521 512 510 C93094 330 354 368 379 391 410 435 455 475 488 C93095 322 333 340 345 353 366 374 389 393 421 C93097 338 344 356 361 378 395 394 407 412 437 1322 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 66 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93098 324 364 357 365 388 382 409 434 443 447 C93099 379 400 414 439 453 478 506 542 542 549 C93100 339 341 366 391 411 418 444 476 470 485 C93101 310 321 332 351 373 376 399 418 424 422 C93102 378 390 404 428 439 451 476 488 484 503 C93103 352 396 424 439 474 476 495 512 540 558 C93104 481 504 498 532 536 535 562 574 549 522 C93105 332 352 362 384 391 405 438 449 449 471 C93106 316 327 344 378 369 374 399 437 442 467 C93107 333 336 342 354 357 364 371 367 373 395 C93108 311 317 321 332 348 C93110 542 542 573 582 611 614 638 641 643 665 GROUP: FEMALE 5 - 20 PPM C93112 342 350 353 362 362 372 C93113 288 290 312 321 330 344 359 371 372 385 C93115 266 284 306 322 335 344 377 388 403 430 C93116 289 296 302 307 320 317 C93117 348 362 387 400 413 422 C93118 307 308 304 318 332 329 336 332 317 358 C93119 263 271 288 296 306 321 331 334 271 297 C93120 277 285 300 309 317 314 C93121 255 259 264 267 277 275 284 286 291 303 C93122 391 402 420 438 468 473 493 501 506 541 C93124 294 291 296 307 315 321 332 340 343 349 C93125 258 264 277 280 283 281 294 306 297 312 C93126 297 304 314 326 345 346 294 C93128 306 301 317 327 332 333 345 349 354 372 C93129 301 311 311 325 340 335 356 365 368 392 C93130 345 343 372 373 401 418 1323 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 67 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93131 341 353 366 383 399 413 435 446 463 479 C93132 341 359 364 383 399 382 399 403 415 427 C93133 291 288 333 339 338 316 362 354 376 389 C93134 329 345 353 365 379 411 447 469 543 C93135 317 321 330 354 351 351 379 387 397 414 C93136 319 325 336 349 353 363 C93137 290 294 304 310 346 347 365 389 399 428 C93138 315 327 335 345 359 387 C93139 274 281 297 311 316 315 332 344 346 361 C93140 385 413 436 455 486 493 509 519 525 534 C93141 342 360 373 387 403 416 432 435 458 479 C93145 274 280 280 295 310 309 322 338 332 332 C93146 263 261 271 277 280 282 299 302 297 312 C93147 287 291 293 309 309 315 C93148 353 359 366 362 386 393 410 419 420 445 C93149 305 313 319 346 366 369 386 401 404 429 C93150 318 321 322 328 358 356 369 372 380 386 C93151 294 312 310 316 314 319 327 330 329 340 C93152 305 314 316 328 332 342 348 329 270 C93153 278 283 297 307 321 335 351 358 368 387 C93154 294 323 332 339 349 342 C93156 292 303 305 320 325 320 335 335 340 351 C93157 294 309 324 351 347 366 390 404 405 421 C93158 322 321 333 340 355 379 392 403 405 431 C93160 392 419 441 471 487 481 502 507 531 529 C93161 329 367 354 386 402 433 453 473 502 509 C93162 326 338 339 339 357 370 376 381 380 390 C93163 292 303 314 323 333 341 C93164 281 288 289 296 306 318 328 335 339 358 C93165 305 316 321 336 342 348 360 375 379 388 C93166 355 356 380 373 388 409 420 430 451 453 1324 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 68 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93167 370 379 C93168 287 287 305 302 310 333 348 354 373 380 C93170 326 338 343 359 369 380 395 403 405 415 C93171 329 342 351 362 370 384 C93172 328 334 341 354 372 370 388 391 406 407 C93173 302 308 318 335 341 348 367 371 373 387 C93174 326 332 338 346 383 396 388 415 424 430 C93175 282 289 289 299 296 302 306 309 307 316 C93176 335 350 357 372 380 396 417 416 427 440 C93177 275 277 278 290 295 296 296 304 310 309 C93178 292 306 304 313 326 334 344 349 355 359 C93179 275 277 285 292 299 295 303 312 315 320 C93180 346 358 371 383 399 403 430 512 GROUP: FEMALE 6 - 20 PPM C93181 304 308 313 315 331 354 359 361 377 388 C93182 282 287 288 299 308 317 345 360 379 398 C93183 356 386 382 387 419 437 456 491 500 528 C93184 267 267 282 289 301 306 334 354 364 397 C93185 317 328 323 326 341 351 360 382 408 436 C93186 268 283 291 306 324 345 384 415 420 444 C93187 376 378 395 404 417 432 457 477 500 521 C93188 347 365 373 409 422 444 486 521 564 594 C93189 263 252 258 275 302 314 322 348 352 355 C93190 328 340 351 368 366 375 408 435 458 480 C93191 358 390 387 416 425 434 465 487 504 529 C93192 294 285 297 308 324 320 333 374 388 405 C93193 372 387 420 454 481 497 531 560 606 631 C93194 339 353 363 370 372 384 405 420 422 445 C93195 325 337 347 369 384 390 428 456 481 501 1325 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 69 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 33 37 41 45 49 53 57 61 65 69 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93196 281 286 290 312 307 322 348 378 390 405 C93197 244 243 265 264 272 271 275 286 285 305 C93198 323 336 350 371 388 397 430 456 470 489 C93199 302 316 324 328 335 341 371 383 316 C93200 280 302 304 318 315 324 348 364 371 392 C93201 275 282 283 295 296 299 320 336 335 352 C93202 275 283 285 287 291 292 311 346 333 349 C93203 346 359 372 392 401 412 467 489 493 519 C93204 297 305 318 329 348 360 393 408 419 439 C93205 317 318 330 338 342 348 354 384 399 407 C93206 309 306 319 329 342 342 382 409 447 479 C93207 280 289 206 C93208 286 311 312 339 350 359 396 433 474 515 C93209 294 295 303 320 334 344 379 412 451 489 C93210 348 364 380 397 419 443 452 504 529 569 C93211 252 260 267 264 274 272 276 298 311 340 C93212 331 358 376 403 420 424 471 521 517 560 C93213 308 310 323 321 313 332 361 382 369 389 C93214 301 316 327 343 355 366 405 429 444 479 C93215 316 324 331 348 354 368 414 447 468 491 C93216 326 341 355 370 375 388 421 439 468 495 C93217 356 367 381 377 388 382 404 427 445 477 C93218 285 294 304 318 326 339 366 376 400 417 C93219 360 349 373 392 393 399 435 482 504 540 C93220 398 385 409 440 436 441 478 496 512 536 1326 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 70 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: C92502 C92503 C92505 C92508 C92510 C92513 C92514 C92515 C92518 C92519 C92520 C92522 C92527 C92530 C92533 C92535 C92536 C92537 C92538 C92539 C92541 C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92554 MALE 1 - 0 PPM 640 497 771 759 729 742 659 498 757 764 743 746 962 972 749 740 891 902 720 739 644 603 818 828 841 842 790 692 928 940 823 827 832 842 1063 1077 1155 951 939 941 958 754 766 733 745 660 672 935 942 850 857 758 676 680 694 954 979 743 742 903 902 697 708 728 744 774 755 986 751 922 565 825 709 947 840 1096 944 971 772 747 672 952 836 570 987 743 903 717 687 756 781 765 1005 764 928 838 904 843 1111 918 989 794 770 675 998 790 1001 755 885 735 612 745 780 757 970 765 920 824 842 1100 839 981 798 772 680 1015 996 749 829 742 581 751 795 769 953 779 945 800 846 1101 780 1018 817 785 675 1008 1010 772 766 752 532 746 807 789 1015 781 814 723 596 1000 835 802 620 1018 765 689 736 478 707 806 786 1056 856 899 832 604 992 756 553 690 445 679 808 789 1070 892 760 821 583 719 1327 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 71 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92557 818 817 783 671 459 C92558 793 661 C92559 769 770 C92560 867 774 C92563 745 743 702 C92565 853 857 853 861 838 C92567 855 854 861 848 826 C92568 806 802 790 799 790 760 722 651 C92569 683 681 682 685 668 688 676 658 644 GROUP: MALE 2 - 0.5 PPM C92571 859 877 910 953 972 1007 926 662 C92572 863 854 858 830 826 792 724 514 C92573 854 818 752 C92576 948 946 982 936 945 928 803 C92577 974 967 982 943 952 977 951 938 934 C92578 973 964 932 776 C92579 820 759 698 557 C92580 808 632 C92581 972 973 947 954 907 833 688 593 364 C92582 675 676 693 701 714 744 731 586 C92583 754 749 766 737 740 735 729 686 624 C92586 529 C92587 840 823 816 652 C92588 685 678 674 665 668 670 631 528 C92589 831 813 782 726 693 632 517 C92590 843 839 836 859 868 876 877 897 887 C92591 811 803 808 822 827 830 C92592 890 883 879 895 C92594 765 764 760 770 798 806 1328 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 72 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92595 713 663 597 C92597 703 692 695 708 694 708 701 710 698 C92598 760 764 770 782 783 787 801 796 781 C92601 802 815 829 839 835 857 859 864 853 C92602 613 593 605 608 620 641 658 662 668 C92604 924 822 716 604 472 C92605 907 894 874 872 871 845 778 678 C92606 777 764 C92607 868 857 820 663 520 C92608 655 469 C92609 799 781 792 809 824 832 843 823 C92611 688 696 722 805 C92612 815 606 C92614 758 746 741 739 740 C92615 933 921 907 865 721 C92617 881 744 561 C92619 679 672 677 685 692 717 711 726 732 C92620 610 615 590 613 617 569 C92622 891 910 901 C92623 686 686 698 713 717 735 739 744 741 C92625 867 776 687 C92626 736 751 718 735 746 768 788 788 788 C92628 465 C92629 788 638 C92630 729 715 656 561 GROUP: MALE 3 - 2 PPM C92631 725 729 742 754 765 786 807 804 808 C92632 846 842 815 792 778 778 772 758 C92633 841 840 846 847 829 859 869 853 871 1329 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 73 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92634 825 828 841 862 C92635 756 605 C92636 875 870 894 908 928 937 937 C92637 702 707 718 726 729 711 673 605 536 C92638 980 917 849 702 575 C92639 725 582 628 489 C92641 740 727 731 743 708 717 712 748 665 C92642 789 783 780 784 786 798 814 829 831 C92643 670 666 666 680 681 688 695 720 741 C92644 705 696 703 692 712 738 744 748 702 C92647 780 768 C92648 706 675 630 524 C92649 685 682 689 697 701 726 711 724 729 C92651 702 572 380 C92653 927 932 945 957 962 965 968 953 823 C92654 743 652 C92655 871 858 845 844 824 710 C92656 857 844 829 813 751 616 461 C92657 782 774 782 792 787 804 806 833 820 C92658 868 865 878 867 888 844 C92659 835 802 777 769 767 779 795 737 748 C92660 622 623 614 640 662 644 663 667 703 C92661 725 684 614 422 429 C92663 940 955 966 983 975 994 C92664 744 608 C92665 936 934 928 888 C92666 645 C92667 895 670 C92669 843 832 838 854 875 889 918 923 858 C92670 422 C92671 776 772 762 803 812 790 1330 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 74 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92672 C92673 C92674 C92675 C92677 C92680 C92681 C92682 C92683 C92685 C92686 C92688 C92690 893 849 890 758 759 558 769 842 913 827 906 627 1042 903 853 900 771 752 555 728 840 923 725 922 597 1035 912 862 919 762 737 524 669 845 939 856 631 1027 917 885 933 766 736 583 846 947 629 1025 911 877 934 762 760 868 953 620 1047 903 845 950 751 767 913 998 516 1043 960 699 760 923 992 413 1052 982 564 941 1002 1076 968 931 947 1073 GROUP: MALE 4 - 5 PPM C92691 785 771 766 755 763 769 771 771 768 C92693 783 773 775 771 793 820 811 750 751 C92694 867 867 876 893 916 936 960 975 968 C92695 727 720 725 725 739 763 772 779 767 C92696 744 729 742 750 778 804 815 824 824 C92698 1006 1021 1016 1015 1041 1077 1091 1060 797 C92699 631 626 635 648 673 680 657 678 674 C92700 604 591 598 603 616 629 637 624 568 C92702 729 745 760 774 801 809 814 751 635 C92704 853 841 848 854 874 905 908 916 903 C92705 824 827 828 832 847 867 866 878 899 C92706 1021 1015 973 931 931 943 888 C92707 719 719 717 729 592 C92708 704 697 707 707 729 765 782 775 755 C92709 649 606 573 502 467 1331 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 75 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92711 804 800 809 819 727 C92712 921 877 768 611 C92716 548 503 506 503 519 498 517 481 495 C92717 828 831 823 831 831 832 760 698 649 C92720 886 908 1019 C92721 695 702 C92723 861 855 C92724 958 953 946 950 958 974 1004 995 997 C92726 733 735 729 730 751 762 690 739 776 C92727 843 843 815 810 776 C92729 698 631 C92730 814 810 813 834 837 838 780 700 588 C92732 834 825 821 C92733 712 693 683 569 418 C92735 791 796 796 811 816 834 829 826 829 C92736 862 865 873 876 888 880 872 901 897 C92737 822 825 837 824 708 C92738 744 733 718 673 649 C92739 686 693 695 712 734 754 770 781 779 C92740 780 739 771 780 808 827 839 876 887 C92741 744 757 738 704 540 C92742 924 938 941 926 919 950 958 963 931 C92743 1046 1075 1089 C92745 1102 1120 1040 872 703 C92746 886 895 C92747 811 803 815 827 750 778 785 761 748 C92748 811 817 813 818 821 830 758 715 620 C92749 744 750 745 759 781 796 818 818 C92750 826 819 823 833 838 843 838 819 702 1332 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 76 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92753 C92756 C92757 C92760 C92761 C92762 C92763 C92764 C92766 C92767 C92768 C92769 C92770 C92771 C92773 C92774 C92775 C92776 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92788 C92791 C92792 C92797 C92800 C92802 736 898 702 551 649 832 1121 594 663 1269 827 832 937 932 559 778 901 768 672 517 893 945 844 780 808 792 797 787 779 682 897 744 901 721 638 822 1113 650 1200 831 824 929 929 558 778 909 763 669 379 911 861 745 784 806 803 795 781 778 682 904 754 893 723 643 803 1118 663 1168 838 828 845 763 579 784 910 744 671 926 792 809 799 803 775 774 677 907 771 770 753 612 786 1140 673 1178 852 833 886 565 786 916 754 690 956 809 814 828 820 795 778 683 933 807 765 589 801 1120 652 1223 849 798 890 555 808 931 757 697 976 811 835 836 825 788 828 696 939 824 753 579 807 932 673 1201 926 788 908 567 811 920 767 689 984 819 840 852 837 798 965 689 938 843 742 574 791 664 1100 777 905 569 810 886 778 667 1013 755 852 874 845 765 688 981 810 701 576 736 655 705 689 580 821 820 788 611 1026 864 892 850 751 678 1014 776 608 564 677 597 797 673 785 543 1017 872 889 859 714 638 988 1333 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 77 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92803 704 704 702 705 706 720 724 734 753 C92804 836 848 841 878 886 892 888 938 951 C92806 728 763 763 776 785 776 720 C92807 849 864 809 702 C92808 626 625 630 632 644 646 635 652 643 C92809 738 759 769 785 798 839 885 901 872 C92810 836 855 847 865 785 667 422 C92813 797 818 788 789 751 740 615 C92814 556 551 567 567 571 588 591 609 621 C92815 643 645 654 686 690 705 713 770 769 C92818 733 726 730 733 731 750 752 716 755 C92819 712 721 722 735 745 775 787 769 GROUP: MALE 6 - 20 PPM C92821 693 718 743 775 788 806 800 799 821 C92822 715 613 496 C92823 758 761 790 792 822 822 850 847 835 C92824 751 613 721 611 651 625 C92825 1119 1112 1068 1048 994 954 C92827 784 778 796 809 813 834 841 849 820 C92828 829 832 840 C92829 923 925 912 847 793 856 902 892 765 C92830 638 604 564 534 489 463 448 C92831 735 712 724 622 C92833 1028 1042 1039 1044 1019 917 920 786 836 C92834 782 778 767 760 751 757 754 719 696 C92835 687 C92836 860 850 874 869 877 C92839 1116 1083 1091 1023 904 C92840 727 717 725 735 747 773 784 676 1334 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 78 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92842 852 851 855 847 827 C92843 978 979 C92844 553 564 542 C92845 684 679 686 680 687 704 C92846 780 768 756 747 656 701 662 618 C92847 978 978 958 946 923 809 644 C92848 502 503 510 466 475 481 463 471 379 C92850 686 705 733 740 738 738 671 654 511 C92851 832 844 852 850 823 828 819 815 827 C92853 824 845 860 874 870 899 907 863 770 C92854 916 930 943 956 915 967 968 925 C92856 682 677 675 675 587 652 663 648 650 C92857 870 868 716 C92858 865 877 879 898 879 764 534 C92860 697 708 728 733 730 751 639 GROUP: FEMALE 1 - 0 PPM C92863 505 500 497 C92866 466 471 488 500 506 518 510 467 386 C92867 513 531 552 567 592 597 603 611 621 C92868 469 474 486 495 521 535 552 580 580 C92869 400 404 423 433 452 450 454 462 471 C92870 505 524 536 571 590 570 535 493 C92871 561 568 600 626 642 672 687 696 673 C92872 458 480 498 495 512 C92873 410 415 443 447 458 459 474 487 470 C92874 525 540 541 568 593 610 608 628 578 C92875 440 370 C92877 507 503 510 519 533 524 533 531 519 C92878 457 461 520 1335 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 79 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92879 511 511 433 376 361 312 C92881 490 473 479 498 499 497 511 517 522 C92882 458 464 479 482 507 496 480 484 469 C92883 486 493 502 470 C92884 429 416 406 367 361 381 C92885 521 529 535 548 558 555 536 524 489 C92886 396 443 681 C92888 683 676 C92890 527 545 553 549 546 541 555 560 540 C92891 401 396 380 353 C92892 678 703 721 749 744 765 759 759 726 C92893 368 372 380 408 C92895 442 451 459 458 462 475 477 485 497 C92896 527 517 533 530 548 547 557 556 563 C92897 384 400 435 424 460 474 463 400 322 C92901 447 342 C92902 805 817 815 807 C92904 428 432 448 468 476 469 477 479 C92906 399 415 415 415 422 410 401 418 371 C92907 420 427 445 459 476 493 492 487 497 C92908 578 587 601 599 508 368 C92909 435 444 442 451 444 459 458 466 482 C92911 380 381 384 373 385 368 381 395 385 C92912 658 677 696 693 715 713 739 749 735 C92913 405 406 419 425 440 454 461 467 438 C92915 539 547 552 563 560 422 C92916 705 708 678 684 569 479 C92918 376 C92924 499 497 491 483 C92925 598 613 633 645 608 558 476 593 599 C92927 415 419 420 429 448 440 440 441 455 1336 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 80 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92928 606 620 585 GROUP: FEMALE 2 - 0.5 PPM C92933 602 598 611 622 624 C92934 469 481 477 403 C92935 638 627 559 472 C92936 415 421 431 437 440 441 432 423 343 C92938 628 642 666 659 682 696 719 735 688 C92940 458 473 511 558 C92943 612 637 655 595 515 391 353 C92946 492 514 509 534 552 566 577 599 592 C92947 455 460 475 483 476 413 C92948 592 595 600 608 612 573 449 C92951 391 393 404 411 422 429 407 413 C92953 391 406 411 419 435 441 453 454 468 C92954 480 501 517 527 564 566 576 464 C92955 612 612 572 518 429 C92956 588 592 614 626 650 663 678 624 C92957 458 465 469 471 493 501 513 519 566 C92958 442 454 449 408 358 C92959 469 492 503 511 547 552 460 C92960 546 564 591 616 634 604 653 674 704 C92961 738 744 729 779 844 844 747 C92963 379 373 372 374 378 353 C92964 485 464 454 473 498 487 495 516 529 C92965 455 452 305 C92970 604 628 646 654 661 633 597 487 367 C92971 347 354 406 473 584 C92972 481 485 513 512 501 394 C92973 571 592 606 634 652 655 687 701 644 1337 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 81 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92974 424 425 440 440 462 450 452 402 363 C92975 440 448 461 487 503 517 515 496 531 C92976 611 679 738 729 733 715 661 529 C92977 525 530 552 549 568 570 571 C92979 477 491 504 522 409 C92983 497 508 528 542 569 588 595 551 402 C92984 531 546 566 572 590 601 597 578 544 C92985 642 681 724 723 743 708 608 C92986 395 402 424 432 458 482 499 503 489 C92988 526 543 540 562 571 591 613 606 617 C92989 444 451 446 C92990 500 519 538 550 550 540 GROUP: FEMALE 3 - 2 PPM C92991 472 498 527 549 557 576 589 595 C92992 512 510 523 C92994 401 417 364 C92997 373 364 354 C92999 500 447 415 333 323 C93001 491 471 402 C93002 502 456 385 C93003 492 504 507 528 561 561 570 537 C93004 393 C93005 480 481 381 349 387 C93007 453 457 470 482 480 511 516 531 C93009 483 477 478 464 443 C93010 558 574 593 575 471 C93011 546 547 559 583 605 624 639 657 C93012 596 605 595 C93013 499 435 364 299 1338 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 82 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93014 489 C93015 478 485 497 503 515 563 495 485 C93016 293 305 278 C93017 512 524 524 534 519 C93022 593 632 647 677 687 681 670 649 C93024 510 507 433 370 C93025 576 584 598 618 632 656 661 628 C93026 412 425 416 415 428 411 397 375 C93027 500 505 543 530 423 C93028 479 494 490 466 427 C93030 581 591 611 619 617 581 C93032 504 388 C93034 412 404 432 C93036 693 745 771 752 C93039 566 579 600 603 597 594 612 618 C93040 372 373 386 384 384 348 359 333 C93041 533 533 439 C93042 673 677 653 590 515 436 C93043 572 583 597 608 610 608 633 C93046 468 490 C93047 803 829 818 849 878 888 918 913 C93048 401 411 419 428 433 445 446 474 C93050 504 522 536 535 546 550 508 GROUP: FEMALE 4 - 5 PPM C93055 634 524 C93056 475 495 519 537 552 527 432 C93057 429 431 438 458 458 428 440 C93059 496 510 520 541 557 584 596 615 621 C93060 465 473 480 480 502 508 510 480 498 1339 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 83 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93061 513 412 C93062 411 405 422 429 428 430 442 432 434 C93063 429 448 465 460 482 504 526 528 529 C93065 367 298 309 292 324 350 C93066 477 481 494 493 528 532 C93068 447 456 449 440 450 430 431 382 C93069 558 565 570 589 611 642 716 685 C93072 472 463 480 481 474 510 519 506 521 C93073 289 C93074 608 622 522 C93076 598 616 621 670 674 570 C93077 511 521 550 588 604 627 640 627 621 C93078 464 479 486 486 510 534 592 601 643 C93079 578 588 600 614 623 619 629 648 623 C93080 469 472 473 500 516 518 530 537 537 C93081 449 458 450 454 457 482 491 479 484 C93082 487 497 509 523 542 569 507 C93083 503 511 536 566 565 575 597 556 508 C93086 513 449 382 346 341 C93087 402 402 422 425 442 467 437 C93088 339 376 C93089 415 408 412 418 417 375 C93090 428 442 453 456 475 498 512 513 453 C93091 553 510 C93092 370 312 C93093 516 521 521 498 480 475 C93094 521 524 533 559 552 536 C93095 421 430 444 449 430 431 420 440 290 C93097 435 442 439 440 424 444 412 C93098 451 470 478 481 482 500 517 519 490 C93099 560 566 580 571 557 537 1340 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 84 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93100 498 514 535 544 535 C93101 440 451 448 450 437 462 484 479 490 C93102 499 506 510 521 532 544 554 545 562 C93103 575 608 634 615 635 644 552 C93104 460 348 281 C93105 408 411 394 310 329 C93106 476 498 502 515 513 518 C93107 397 392 403 418 421 437 468 474 494 C93110 686 666 660 620 483 GROUP: FEMALE 5 - 20 PPM C93113 408 425 442 446 472 507 525 546 580 C93115 434 463 482 507 518 541 562 528 507 C93118 363 407 379 433 422 392 C93119 313 330 341 339 361 C93121 269 215 C93122 599 600 605 606 543 C93124 359 357 342 336 323 356 354 390 432 C93125 314 313 308 308 307 298 292 276 301 C93128 378 380 398 419 430 436 446 C93129 396 396 410 425 429 396 335 C93131 485 494 482 487 503 467 C93132 437 455 458 485 493 472 445 445 265 C93133 395 404 402 414 413 416 424 426 425 C93135 365 391 368 381 398 416 435 448 473 C93137 455 460 507 494 504 496 482 467 446 C93139 364 363 374 373 366 352 345 332 329 C93140 542 537 545 559 563 571 565 548 514 C93141 486 488 512 534 537 545 530 C93145 328 1341 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 85 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93146 323 330 334 342 353 352 360 373 356 C93148 462 462 479 492 505 C93149 434 429 441 432 449 447 472 413 338 C93150 384 378 352 360 341 C93151 335 335 336 339 345 377 401 456 526 C93153 407 410 403 416 421 410 486 452 463 C93156 335 262 362 345 C93157 440 458 469 485 499 513 552 572 583 C93158 439 429 438 457 455 465 459 464 485 C93160 408 C93161 543 563 557 583 583 652 687 700 729 C93162 408 411 411 413 433 439 452 447 429 C93164 367 381 390 410 416 430 457 464 476 C93165 399 415 421 426 424 438 463 462 464 C93166 461 471 464 486 476 496 509 522 511 C93168 381 387 391 399 395 400 419 413 407 C93170 424 427 441 446 433 383 C93172 341 261 C93173 396 407 401 385 437 473 504 492 C93174 433 445 458 461 476 485 485 494 495 C93175 320 319 318 306 316 C93176 449 450 424 377 C93177 315 320 320 322 327 331 342 346 355 C93178 367 379 377 381 388 396 403 396 394 C93179 322 318 325 308 307 316 331 326 331 GROUP: FEMALE 6 - 20 PPM C93181 405 407 419 431 436 448 461 470 472 C93182 434 467 491 508 523 538 560 568 559 C93183 547 578 600 621 646 678 707 749 804 1342 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 86 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93184 432 443 465 490 518 544 559 580 583 C93185 458 468 480 503 506 528 554 549 569 C93186 457 C93187 535 545 553 567 496 566 557 491 C93188 617 645 666 694 715 720 721 687 709 C93189 380 380 386 301 C93190 507 518 526 546 543 523 441 340 330 C93191 546 553 563 596 581 C93192 433 464 467 496 519 537 569 570 551 C93193 632 C93194 450 450 424 358 346 C93196 432 432 433 413 C93197 308 305 324 324 324 350 356 362 369 C93198 511 519 536 547 544 569 574 579 590 C93200 415 421 434 454 485 474 C93201 365 389 399 411 421 448 449 453 472 C93202 384 382 390 419 427 441 462 459 472 C93203 544 526 509 460 423 C93204 428 438 442 470 484 C93205 430 451 472 486 501 509 540 520 463 C93206 504 525 553 566 580 594 503 388 C93208 548 571 575 C93209 505 540 541 576 585 584 614 634 638 C93210 593 608 619 658 700 677 604 471 337 C93211 361 357 376 404 409 426 424 425 383 C93212 580 568 519 C93213 410 376 310 C93214 512 531 550 572 611 620 635 C93215 511 501 416 335 C93216 505 531 541 535 551 549 578 579 577 C93217 503 517 529 583 604 1343 Appendix 3 Covance 6329-183 3M T-6295 Individual Body Weight Data (g) PAGE: 87 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 73 77 81 85 89 93 97 101 105 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93218 428 442 453 458 470 C93219 560 577 588 622 651 683 733 763 794 C93220 553 568 584 595 599 608 618 624 620 1344 APPENDIX 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) Individual Test Material Consumption Data (mg/kg/day) 1345 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92501 C92502 C92503 C92504 C92505 C92506 C92507 C92508 C92509 C92510 C92511 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92521 C92522 C92523 C92524 C92525 C92526 C92527 C92528 C92529 C92530 C92531 178.00 164.00 166.00 169.00 163.00 160.00 169.00 160.00 157.00 177.00 179.00 166.00 165.00 179.00 151.00 156.00 180.00 167.00 154.00 154.00 164.00 164.00 148.00 190.00 166.00 181.00 168.00 151.00 159.00 SPILLED 168.00 203.00 166.00 178.00 181.00 SPILLED 171.00 196.00 165.00 164.00 SPILLED 192.00 179.00 SPILLED 200.00 164.00 168.00 192.00 191.00 160.00 181.00 170.00 177.00 158.00 200.00 169.00 186.00 SPILLED 159.00 179.00 SPILLED 181.00 212.00 180.00 191.00 177.00 175.00 182.00 191.00 177.00 172.00 205.00 202.00 181.00 191.00 205.00 174.00 176.00 202.00 183.00 173.00 196.00 188.00 175.00 168.00 199.00 182.00 196.00 198.00 170.00 175.00 SPILLED 204.00 217.00 185.00 203.00 184.00 SPILLED NOT TAKEN 195.00 174.00 180.00 218.00 210.00 181.00 SPILLED 211.00 181.00 180.00 213.00 196.00 175.00 187.00 186.00 179.00 172.00 NOT TAKEN 187.00 NOT TAKEN 195.00 183.00 NOT TAKEN SPILLED SPILLED 214.00 178.00 192.00 187.00 SPILLED 189.00 175.00 174.00 213.00 SPILLED 187.00 SPILLED 203.00 176.00 176.00 218.00 196.00 171.00 189.00 203.00 169.00 173.00 190.00 188.00 180.00 223.00 206.00 218.00 179.00 SPILLED 186.00 201.00 189.00 182.00 169.00 208.00 235.00 196.00 SPILLED 208.00 175.00 179.00 215.00 199.00 180.00 190.00 198.00 180.00 178.00 189.00 189.00 190.00 SPILLED SPILLED 218.00 176.00 194.00 203.00 194.00 185.00 182.00 173.00 209.00 SPILLED 190.00 SPILLED 207.00 185.00 171.00 215.00 200.00 176.00 193.00 191.00 165.00 175.00 181.00 185.00 192.00 SPILLED SPILLED 219.00 180.00 192.00 199.00 SPILLED 181.00 174.00 167.00 210.00 SPILLED 180.00 SPILLED 204.00 184.00 165.00 209.00 198.00 169.00 SPILLED SPILLED 171.00 176.00 183.00 186.00 176.00 SPILLED SPILLED 218.00 178.00 197.00 193.00 184.00 178.00 174.00 178.00 199.00 231.00 183.00 191.00 206.00 182.00 171.00 210.00 205.00 169.00 196.00 185.00 167.00 178.00 183.00 196.00 173.00 SPILLED SPILLED 222.00 181.00 191.00 194.00 SPILLED 184.00 177.00 174.00 193.00 SPILLED 192.00 185.00 213.00 182.00 166.00 207.00 211.00 172.00 187.00 197.00 169.00 169.00 181.00 190.00 SPILLED SPILLED SPILLED 1346 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92532 C92533 C92534 C92535 C92536 C92537 C92538 C92539 C92540 C92541 C92542 C92543 C92544 C92545 C92546 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 200.00 193.00 181.00 173.00 165.00 177.00 160.00 181.00 176.00 173.00 171.00 169.00 166.00 177.00 163.00 178.00 171.00 149.00 146.00 155.00 168.00 136.00 150.00 161.00 157.00 156.00 171.00 181.00 161.00 143.00 219.00 215.00 206.00 170.00 185.00 180.00 190.00 175.00 188.00 190.00 183.00 185.00 171.00 SPILLED 177.00 171.00 195.00 169.00 157.00 161.00 166.00 177.00 145.00 154.00 180.00 185.00 170.00 190.00 183.00 172.00 158.00 188.00 SPILLED 206.00 178.00 194.00 187.00 202.00 183.00 191.00 190.00 195.00 184.00 167.00 196.00 176.00 172.00 199.00 168.00 164.00 175.00 159.00 176.00 157.00 153.00 183.00 181.00 178.00 202.00 183.00 176.00 166.00 186.00 234.00 206.00 191.00 205.00 198.00 208.00 191.00 204.00 204.00 198.00 173.00 159.00 189.00 184.00 NOT TAKEN 210.00 162.00 166.00 188.00 165.00 195.00 SPILLED 162.00 242.00 189.00 187.00 202.00 182.00 176.00 180.00 183.00 241.00 211.00 186.00 202.00 197.00 212.00 195.00 202.00 206.00 193.00 196.00 178.00 182.00 190.00 209.00 178.00 170.00 195.00 160.00 189.00 152.00 156.00 186.00 180.00 188.00 204.00 188.00 175.00 179.00 182.00 SPILLED SPILLED 192.00 SPILLED 197.00 219.00 197.00 207.00 210.00 193.00 184.00 178.00 184.00 196.00 218.00 184.00 SPILLED 167.00 171.00 185.00 153.00 163.00 210.00 185.00 190.00 207.00 188.00 182.00 186.00 SPILLED SPILLED 213.00 187.00 228.00 189.00 213.00 197.00 221.00 217.00 199.00 189.00 177.00 189.00 199.00 214.00 187.00 175.00 210.00 176.00 193.00 153.00 162.00 211.00 179.00 193.00 215.00 186.00 186.00 174.00 SPILLED 241.00 211.00 183.00 215.00 174.00 220.00 200.00 220.00 207.00 195.00 189.00 175.00 134.00 196.00 214.00 SPILLED 165.00 206.00 170.00 189.00 153.00 158.00 201.00 179.00 192.00 205.00 185.00 182.00 182.00 SPILLED SPILLED 212.00 188.00 213.00 184.00 218.00 195.00 218.00 211.00 200.00 185.00 170.00 128.00 198.00 205.00 SPILLED 165.00 209.00 170.00 191.00 160.00 157.00 SPILLED 174.00 SPILLED 203.00 189.00 184.00 186.00 185.00 225.00 202.00 177.00 201.00 175.00 209.00 188.00 216.00 206.00 194.00 181.00 166.00 124.00 190.00 202.00 181.00 167.00 207.00 166.00 189.00 148.00 158.00 204.00 177.00 SPILLED 210.00 183.00 180.00 185.00 191.00 1347 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92563 C92564 C92565 C92566 C92567 C92568 C92569 C92570 169.00 166.00 164.00 165.00 157.00 152.00 162.00 171.00 SPILLED 174.00 178.00 172.00 173.00 163.00 163.00 SPILLED 190.00 178.00 181.00 195.00 180.00 177.00 172.00 187.00 200.00 186.00 184.00 203.00 192.00 182.00 182.00 194.00 197.00 195.00 193.00 206.00 194.00 185.00 192.00 199.00 205.00 199.00 198.00 192.00 195.00 189.00 195.00 196.00 201.00 194.00 200.00 193.00 194.00 193.00 198.00 198.00 202.00 189.00 SPILLED 196.00 190.00 192.00 SPILLED 198.00 208.00 192.00 208.00 201.00 189.00 198.00 185.00 198.00 204.00 187.00 202.00 199.00 181.00 195.00 189.00 191.00 GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 148.00 163.00 175.00 167.00 158.00 172.00 168.00 175.00 173.00 165.00 166.00 147.00 161.00 SPILLED 179.00 147.00 177.00 SPILLED 171.00 180.00 169.00 173.00 195.00 179.00 CNTMD 178.00 181.00 175.00 186.00 176.00 175.00 160.00 SPILLED 159.00 CNTMD 150.00 178.00 164.00 182.00 194.00 185.00 179.00 SPILLED SPILLED 159.00 185.00 194.00 175.00 199.00 183.00 188.00 SPILLED 169.00 166.00 186.00 167.00 187.00 173.00 197.00 204.00 183.00 198.00 195.00 NOT TAKEN NOT TAKEN 191.00 200.00 180.00 203.00 185.00 196.00 SPILLED SPILLED 167.00 168.00 164.00 187.00 176.00 198.00 212.00 182.00 197.00 224.00 194.00 196.00 185.00 208.00 192.00 190.00 157.00 SPILLED 166.00 197.00 185.00 213.00 193.00 206.00 208.00 196.00 198.00 233.00 191.00 SPILLED SPILLED 209.00 SPILLED 196.00 161.00 SPILLED 167.00 203.00 186.00 208.00 194.00 207.00 SPILLED SPILLED 205.00 SPILLED 189.00 219.00 217.00 221.00 SPILLED 205.00 171.00 SPILLED 164.00 CNTMD 193.00 223.00 SPILLED 211.00 174.00 205.00 195.00 214.00 189.00 200.00 193.00 217.00 187.00 201.00 163.00 SPILLED 164.00 207.00 188.00 212.00 SPILLED 201.00 209.00 SPILLED 203.00 218.00 189.00 190.00 190.00 203.00 189.00 193.00 162.00 171.00 166.00 192.00 183.00 204.00 SPILLED 201.00 212.00 223.00 210.00 203.00 188.00 198.00 201.00 200.00 SPILLED 188.00 155.00 176.00 162.00 199.00 186.00 201.00 SPILLED 198.00 212.00 1348 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 148.00 148.00 167.00 163.00 155.00 167.00 159.00 166.00 181.00 163.00 147.00 161.00 176.00 163.00 167.00 144.00 165.00 186.00 168.00 164.00 164.00 150.00 174.00 175.00 181.00 139.00 176.00 168.00 161.00 151.00 172.00 150.00 162.00 178.00 176.00 169.00 168.00 161.00 172.00 193.00 162.00 159.00 163.00 SPILLED 178.00 169.00 157.00 181.00 188.00 177.00 174.00 170.00 154.00 189.00 188.00 180.00 141.00 188.00 195.00 SPILLED 167.00 181.00 163.00 173.00 177.00 SPILLED 192.00 178.00 172.00 194.00 196.00 175.00 176.00 170.00 SPILLED 186.00 178.00 180.00 199.00 209.00 187.00 SPILLED 177.00 171.00 194.00 198.00 196.00 155.00 202.00 SPILLED 191.00 178.00 190.00 170.00 184.00 185.00 199.00 178.00 184.00 175.00 193.00 193.00 180.00 182.00 191.00 SPILLED 190.00 179.00 177.00 204.00 208.00 187.00 NOT TAKEN 182.00 172.00 NOT TAKEN SPILLED 168.00 SPILLED 209.00 NOT TAKEN 185.00 SPILLED 198.00 170.00 182.00 178.00 204.00 184.00 191.00 199.00 193.00 214.00 184.00 175.00 184.00 196.00 188.00 190.00 182.00 208.00 SPILLED 190.00 185.00 176.00 223.00 189.00 SPILLED 217.00 181.00 174.00 214.00 164.00 182.00 178.00 211.00 192.00 194.00 194.00 199.00 SPILLED 186.00 174.00 178.00 SPILLED 194.00 SPILLED 184.00 203.00 SPILLED 192.00 179.00 169.00 224.00 194.00 157.00 218.00 181.00 175.00 208.00 SPILLED 189.00 180.00 212.00 191.00 191.00 190.00 203.00 SPILLED 190.00 172.00 179.00 SPILLED 198.00 196.00 183.00 SPILLED SPILLED 194.00 177.00 175.00 232.00 197.00 163.00 221.00 191.00 176.00 209.00 SPILLED 186.00 168.00 207.00 187.00 190.00 186.00 196.00 215.00 182.00 170.00 SPILLED SPILLED 195.00 190.00 189.00 SPILLED SPILLED 185.00 173.00 181.00 SPILLED 194.00 167.00 213.00 179.00 179.00 216.00 163.00 182.00 171.00 SPILLED 180.00 190.00 SPILLED 192.00 218.00 181.00 166.00 183.00 196.00 195.00 194.00 193.00 207.00 214.00 180.00 169.00 175.00 SPILLED 188.00 SPILLED 210.00 179.00 170.00 211.00 166.00 186.00 172.00 204.00 182.00 192.00 187.00 190.00 211.00 177.00 160.00 175.00 195.00 192.00 190.00 193.00 206.00 222.00 178.00 170.00 183.00 SPILLED 181.00 170.00 199.00 175.00 150.00 203.00 1349 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 CNTMD 143.00 CNTMD 163.00 175.00 177.00 168.00 164.00 187.00 166.00 153.00 150.00 SPILLED 176.00 188.00 180.00 178.00 188.00 175.00 157.00 166.00 SPILLED 183.00 203.00 190.00 191.00 197.00 187.00 169.00 167.00 SPILLED 193.00 SPILLED 219.00 216.00 197.00 185.00 171.00 172.00 SPILLED 190.00 234.00 SPILLED 214.00 202.00 189.00 174.00 169.00 SPILLED 187.00 SPILLED 220.00 SPILLED 204.00 188.00 174.00 177.00 SPILLED 201.00 SPILLED 215.00 213.00 229.00 187.00 153.00 176.00 SPILLED 205.00 SPILLED 215.00 215.00 242.00 183.00 155.00 178.00 213.00 202.00 SPILLED SPILLED 212.00 222.00 176.00 164.00 177.00 SPILLED 202.00 SPILLED SPILLED SPILLED 225.00 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 166.00 192.00 154.00 149.00 192.00 200.00 137.00 173.00 151.00 159.00 154.00 168.00 156.00 171.00 169.00 197.00 151.00 155.00 140.00 174.00 193.00 160.00 162.00 205.00 SPILLED 145.00 182.00 156.00 171.00 175.00 176.00 160.00 171.00 169.00 194.00 168.00 165.00 153.00 176.00 200.00 SPILLED 172.00 209.00 197.00 152.00 200.00 163.00 169.00 176.00 187.00 168.00 173.00 SPILLED 190.00 173.00 171.00 159.00 180.00 187.00 148.00 SPILLED SPILLED SPILLED 161.00 211.00 166.00 170.00 192.00 196.00 170.00 174.00 185.00 NOT TAKEN 171.00 169.00 165.00 180.00 211.00 170.00 181.00 205.00 SPILLED 154.00 218.00 176.00 164.00 187.00 196.00 165.00 169.00 185.00 172.00 164.00 167.00 180.00 209.00 178.00 179.00 213.00 204.00 156.00 223.00 185.00 165.00 189.00 200.00 164.00 170.00 190.00 173.00 166.00 173.00 180.00 209.00 181.00 178.00 SPILLED 212.00 153.00 227.00 SPILLED 172.00 183.00 188.00 162.00 158.00 182.00 171.00 157.00 173.00 187.00 214.00 181.00 179.00 201.00 227.00 159.00 228.00 186.00 176.00 188.00 184.00 165.00 163.00 185.00 172.00 161.00 166.00 172.00 202.00 179.00 171.00 197.00 SPILLED 146.00 223.00 171.00 164.00 185.00 180.00 165.00 163.00 184.00 167.00 154.00 162.00 176.00 208.00 179.00 182.00 SPILLED SPILLED 152.00 226.00 176.00 172.00 192.00 183.00 168.00 168.00 196.00 171.00 166.00 173.00 1350 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 160.00 176.00 159.00 158.00 170.00 167.00 152.00 164.00 161.00 182.00 173.00 164.00 149.00 180.00 165.00 183.00 176.00 167.00 162.00 154.00 163.00 154.00 144.00 176.00 174.00 178.00 175.00 149.00 143.00 168.00 169.00 176.00 189.00 173.00 168.00 187.00 184.00 160.00 188.00 170.00 193.00 167.00 168.00 163.00 201.00 185.00 190.00 184.00 185.00 172.00 165.00 172.00 165.00 149.00 170.00 174.00 183.00 172.00 157.00 158.00 173.00 192.00 180.00 194.00 183.00 175.00 202.00 201.00 169.00 183.00 187.00 195.00 194.00 174.00 187.00 215.00 187.00 201.00 198.00 205.00 183.00 178.00 177.00 183.00 157.00 175.00 170.00 188.00 SPILLED 160.00 166.00 177.00 199.00 NOT TAKEN 194.00 NOT TAKEN 182.00 203.00 208.00 184.00 176.00 216.00 207.00 221.00 175.00 SPILLED 230.00 SPILLED 207.00 203.00 209.00 NOT TAKEN 184.00 177.00 186.00 160.00 175.00 182.00 184.00 154.00 160.00 NOT TAKEN 180.00 195.00 197.00 179.00 SPILLED 221.00 187.00 SPILLED 189.00 203.00 178.00 172.00 176.00 SPILLED 197.00 207.00 206.00 210.00 184.00 182.00 186.00 153.00 185.00 171.00 183.00 188.00 164.00 204.00 207.00 187.00 177.00 SPILLED 223.00 183.00 SPILLED SPILLED 206.00 197.00 172.00 173.00 SPILLED 197.00 208.00 203.00 213.00 188.00 181.00 191.00 170.00 182.00 SPILLED 183.00 176.00 SPILLED 187.00 SPILLED 190.00 178.00 SPILLED SPILLED 189.00 197.00 194.00 211.00 180.00 170.00 170.00 SPILLED 207.00 207.00 207.00 218.00 190.00 177.00 152.00 177.00 184.00 SPILLED 183.00 178.00 152.00 186.00 194.00 192.00 184.00 203.00 SPILLED 186.00 SPILLED 198.00 205.00 190.00 177.00 175.00 SPILLED 196.00 213.00 206.00 221.00 197.00 177.00 188.00 180.00 180.00 SPILLED 179.00 178.00 165.00 195.00 SPILLED 193.00 188.00 190.00 SPILLED 181.00 189.00 184.00 203.00 176.00 157.00 168.00 203.00 191.00 211.00 204.00 209.00 194.00 165.00 183.00 173.00 179.00 204.00 184.00 168.00 169.00 193.00 208.00 192.00 183.00 SPILLED SPILLED 184.00 196.00 199.00 211.00 SPILLED 167.00 175.00 SPILLED 192.00 210.00 209.00 213.00 197.00 177.00 194.00 185.00 SPILLED 216.00 186.00 169.00 172.00 196.00 215.00 1351 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 152.00 150.00 170.00 163.00 151.00 161.00 170.00 155.00 155.00 194.00 SPILLED 165.00 171.00 160.00 155.00 165.00 167.00 157.00 164.00 210.00 167.00 173.00 185.00 167.00 172.00 176.00 177.00 168.00 173.00 SPILLED SPILLED 164.00 182.00 162.00 179.00 162.00 188.00 178.00 174.00 228.00 175.00 174.00 189.00 180.00 186.00 180.00 202.00 SPILLED 180.00 217.00 SPILLED 174.00 192.00 177.00 182.00 183.00 202.00 SPILLED 187.00 206.00 SPILLED 172.00 186.00 174.00 191.00 185.00 201.00 SPILLED 182.00 133.00 SPILLED 180.00 SPILLED 180.00 196.00 182.00 205.00 SPILLED 173.00 230.00 SPILLED 170.00 180.00 172.00 190.00 177.00 199.00 SPILLED 158.00 218.00 SPILLED 180.00 187.00 175.00 198.00 180.00 202.00 SPILLED 164.00 220.00 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 161.00 148.00 175.00 163.00 182.00 166.00 167.00 180.00 148.00 151.00 154.00 161.00 153.00 157.00 151.00 165.00 149.00 172.00 164.00 151.00 SPILLED 156.00 181.00 170.00 175.00 175.00 149.00 157.00 161.00 166.00 170.00 165.00 154.00 174.00 162.00 178.00 174.00 173.00 187.00 163.00 184.00 178.00 187.00 189.00 157.00 155.00 173.00 172.00 187.00 175.00 169.00 174.00 164.00 184.00 172.00 167.00 194.00 163.00 SPILLED SPILLED 182.00 189.00 157.00 156.00 164.00 173.00 192.00 180.00 176.00 173.00 157.00 172.00 177.00 173.00 202.00 SPILLED 190.00 SPILLED SPILLED 197.00 158.00 149.00 171.00 178.00 194.00 185.00 184.00 174.00 157.00 158.00 189.00 186.00 202.00 176.00 SPILLED SPILLED 197.00 191.00 166.00 150.00 169.00 171.00 203.00 186.00 178.00 176.00 152.00 162.00 193.00 183.00 198.00 182.00 194.00 200.00 196.00 SPILLED 149.00 148.00 174.00 176.00 204.00 190.00 191.00 172.00 155.00 171.00 181.00 182.00 191.00 183.00 SPILLED SPILLED SPILLED 195.00 158.00 157.00 171.00 171.00 205.00 191.00 189.00 174.00 157.00 174.00 185.00 179.00 192.00 185.00 172.00 SPILLED 213.00 194.00 160.00 154.00 177.00 176.00 205.00 194.00 195.00 179.00 165.00 179.00 181.00 177.00 193.00 181.00 177.00 SPILLED 196.00 191.00 168.00 150.00 162.00 168.00 205.00 184.00 195.00 171.00 151.00 173.00 1352 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 176.00 158.00 153.00 182.00 199.00 175.00 168.00 179.00 171.00 164.00 151.00 200.00 161.00 174.00 172.00 201.00 158.00 158.00 151.00 163.00 170.00 158.00 SPILLED 159.00 159.00 170.00 173.00 157.00 151.00 140.00 144.00 180.00 170.00 155.00 188.00 SPILLED SPILLED SPILLED 186.00 177.00 176.00 162.00 SPILLED 176.00 179.00 197.00 215.00 170.00 173.00 166.00 173.00 191.00 170.00 SPILLED 177.00 166.00 179.00 180.00 164.00 160.00 157.00 148.00 175.00 176.00 168.00 189.00 SPILLED 203.00 184.00 197.00 187.00 191.00 169.00 SPILLED 182.00 187.00 SPILLED 216.00 173.00 186.00 178.00 201.00 214.00 186.00 173.00 195.00 175.00 184.00 180.00 176.00 174.00 180.00 159.00 165.00 180.00 161.00 186.00 SPILLED 184.00 NOT TAKEN 179.00 179.00 NOT TAKEN 169.00 SPILLED 147.00 186.00 SPILLED 216.00 154.00 183.00 SPILLED 201.00 210.00 SPILLED NOT TAKEN 174.00 165.00 NOT TAKEN 182.00 170.00 167.00 170.00 160.00 187.00 SPILLED 173.00 200.00 182.00 193.00 203.00 179.00 173.00 SPILLED 156.00 196.00 SPILLED 222.00 179.00 191.00 187.00 207.00 217.00 186.00 196.00 168.00 189.00 177.00 176.00 182.00 167.00 187.00 192.00 172.00 204.00 SPILLED 192.00 207.00 186.00 176.00 SPILLED 171.00 189.00 SPILLED 230.00 172.00 188.00 190.00 215.00 209.00 188.00 189.00 163.00 189.00 177.00 172.00 181.00 164.00 195.00 SPILLED 181.00 SPILLED SPILLED SPILLED 211.00 190.00 179.00 SPILLED 173.00 192.00 SPILLED SPILLED 168.00 192.00 190.00 215.00 209.00 189.00 195.00 162.00 192.00 176.00 172.00 188.00 167.00 185.00 SPILLED 174.00 197.00 SPILLED SPILLED 205.00 186.00 177.00 SPILLED 173.00 192.00 207.00 217.00 166.00 192.00 SPILLED 208.00 206.00 178.00 191.00 167.00 186.00 175.00 171.00 181.00 167.00 179.00 SPILLED 174.00 SPILLED SPILLED 210.00 203.00 192.00 SPILLED SPILLED 178.00 200.00 SPILLED 223.00 SPILLED 187.00 187.00 202.00 213.00 179.00 198.00 180.00 190.00 186.00 184.00 191.00 184.00 170.00 192.00 168.00 201.00 199.00 206.00 198.00 179.00 181.00 SPILLED 161.00 145.00 SPILLED SPILLED 172.00 183.00 185.00 215.00 205.00 182.00 196.00 177.00 184.00 179.00 177.00 190.00 177.00 1353 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 169.00 156.00 176.00 164.00 152.00 170.00 182.00 158.00 160.00 149.00 167.00 178.00 163.00 190.00 183.00 178.00 183.00 201.00 163.00 165.00 152.00 180.00 185.00 167.00 203.00 191.00 193.00 197.00 202.00 175.00 177.00 160.00 198.00 183.00 176.00 199.00 198.00 NOT TAKEN 200.00 185.00 179.00 181.00 160.00 190.00 184.00 177.00 205.00 206.00 207.00 206.00 189.00 192.00 167.00 193.00 185.00 180.00 205.00 204.00 210.00 SPILLED 196.00 201.00 176.00 188.00 199.00 198.00 204.00 208.00 210.00 SPILLED 195.00 228.00 177.00 SPILLED 196.00 SPILLED 208.00 SPILLED 205.00 SPILLED 197.00 SPILLED 172.00 191.00 196.00 178.00 208.00 210.00 205.00 198.00 SPILLED 225.00 178.00 179.00 181.00 176.00 204.00 208.00 198.00 190.00 SPILLED SPILLED SPILLED 178.00 GROUP: MALE 5 - 20 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 150.00 145.00 184.00 141.00 148.00 167.00 149.00 138.00 154.00 166.00 155.00 160.00 171.00 190.00 156.00 162.00 154.00 172.00 146.00 194.00 154.00 154.00 187.00 154.00 153.00 161.00 180.00 176.00 165.00 178.00 207.00 170.00 172.00 177.00 189.00 158.00 197.00 167.00 174.00 213.00 170.00 163.00 173.00 191.00 174.00 178.00 195.00 218.00 181.00 174.00 197.00 189.00 NOT TAKEN 204.00 164.00 164.00 217.00 179.00 161.00 NOT TAKEN 189.00 180.00 184.00 189.00 201.00 183.00 172.00 195.00 191.00 210.00 166.00 171.00 217.00 180.00 165.00 189.00 176.00 185.00 199.00 220.00 189.00 178.00 188.00 186.00 192.00 164.00 164.00 226.00 182.00 171.00 187.00 178.00 189.00 193.00 220.00 188.00 175.00 204.00 SPILLED 206.00 177.00 170.00 SPILLED SPILLED 169.00 196.00 172.00 183.00 205.00 218.00 198.00 176.00 206.00 SPILLED 201.00 159.00 164.00 SPILLED SPILLED 163.00 185.00 176.00 SPILLED 202.00 222.00 187.00 174.00 221.00 182.00 205.00 159.00 176.00 SPILLED 175.00 160.00 183.00 170.00 173.00 203.00 SPILLED 187.00 177.00 225.00 182.00 173.00 163.00 172.00 SPILLED 172.00 157.00 186.00 171.00 177.00 200.00 207.00 187.00 176.00 224.00 1354 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 167.00 169.00 177.00 162.00 167.00 147.00 160.00 170.00 170.00 153.00 157.00 147.00 146.00 160.00 151.00 172.00 161.00 154.00 151.00 174.00 183.00 143.00 161.00 150.00 156.00 163.00 151.00 148.00 137.00 149.00 161.00 187.00 177.00 SPILLED 167.00 174.00 160.00 171.00 186.00 181.00 161.00 182.00 155.00 166.00 170.00 160.00 178.00 164.00 167.00 156.00 181.00 204.00 151.00 164.00 153.00 183.00 160.00 153.00 157.00 142.00 154.00 158.00 197.00 200.00 190.00 194.00 194.00 167.00 173.00 202.00 185.00 165.00 192.00 163.00 155.00 172.00 179.00 194.00 187.00 180.00 169.00 199.00 213.00 169.00 177.00 174.00 179.00 164.00 169.00 165.00 149.00 161.00 170.00 195.00 186.00 181.00 207.00 177.00 167.00 175.00 197.00 185.00 164.00 190.00 NOT TAKEN 160.00 172.00 178.00 190.00 167.00 180.00 165.00 201.00 SPILLED 168.00 175.00 175.00 176.00 NOT TAKEN 156.00 165.00 154.00 162.00 NOT TAKEN 203.00 191.00 191.00 SPILLED 182.00 168.00 189.00 208.00 189.00 168.00 180.00 164.00 172.00 184.00 198.00 193.00 185.00 173.00 196.00 219.00 171.00 179.00 170.00 188.00 162.00 167.00 158.00 162.00 208.00 189.00 182.00 188.00 184.00 157.00 192.00 204.00 188.00 162.00 183.00 165.00 175.00 180.00 200.00 189.00 190.00 172.00 194.00 197.00 172.00 181.00 174.00 186.00 166.00 168.00 162.00 164.00 200.00 197.00 193.00 SPILLED 175.00 159.00 194.00 211.00 187.00 173.00 189.00 172.00 176.00 190.00 201.00 175.00 189.00 180.00 202.00 200.00 177.00 185.00 181.00 190.00 170.00 175.00 155.00 166.00 180.00 189.00 185.00 175.00 174.00 155.00 189.00 207.00 186.00 163.00 185.00 171.00 138.00 170.00 192.00 179.00 SPILLED 178.00 138.00 167.00 163.00 177.00 175.00 187.00 167.00 180.00 153.00 166.00 188.00 188.00 183.00 176.00 182.00 163.00 196.00 195.00 185.00 166.00 183.00 172.00 168.00 177.00 181.00 178.00 181.00 166.00 SPILLED 198.00 168.00 175.00 164.00 181.00 163.00 178.00 148.00 163.00 181.00 188.00 184.00 172.00 176.00 150.00 185.00 202.00 186.00 169.00 186.00 SPILLED 168.00 176.00 186.00 181.00 183.00 175.00 SPILLED 194.00 SPILLED 176.00 170.00 182.00 161.00 177.00 148.00 167.00 1355 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 158.00 166.00 166.00 157.00 164.00 155.00 172.00 159.00 148.00 145.00 151.00 175.00 164.00 153.00 176.00 146.00 159.00 129.00 164.00 155.00 151.00 SPILLED 156.00 178.00 173.00 161.00 171.00 153.00 168.00 168.00 163.00 150.00 171.00 177.00 175.00 160.00 180.00 152.00 149.00 137.00 174.00 156.00 152.00 167.00 169.00 184.00 190.00 166.00 179.00 167.00 181.00 178.00 173.00 169.00 174.00 185.00 190.00 165.00 183.00 162.00 155.00 147.00 187.00 160.00 163.00 163.00 163.00 SPILLED 197.00 167.00 185.00 166.00 167.00 178.00 159.00 164.00 174.00 180.00 173.00 162.00 185.00 156.00 SPILLED 146.00 189.00 164.00 158.00 170.00 184.00 183.00 204.00 172.00 193.00 180.00 195.00 183.00 191.00 167.00 190.00 188.00 204.00 172.00 185.00 153.00 172.00 153.00 195.00 169.00 157.00 181.00 190.00 179.00 194.00 175.00 185.00 171.00 187.00 174.00 181.00 158.00 177.00 180.00 200.00 181.00 181.00 151.00 136.00 151.00 194.00 168.00 161.00 185.00 183.00 SPILLED 193.00 178.00 184.00 169.00 194.00 179.00 186.00 164.00 184.00 182.00 202.00 182.00 198.00 145.00 164.00 154.00 199.00 164.00 160.00 181.00 176.00 178.00 191.00 176.00 182.00 174.00 183.00 143.00 184.00 153.00 184.00 187.00 198.00 206.00 191.00 145.00 188.00 154.00 210.00 178.00 167.00 177.00 180.00 SPILLED SPILLED 168.00 181.00 175.00 187.00 119.00 174.00 151.00 188.00 177.00 194.00 163.00 182.00 134.00 180.00 141.00 192.00 167.00 153.00 181.00 184.00 SPILLED 185.00 172.00 176.00 173.00 187.00 151.00 168.00 158.00 179.00 175.00 199.00 172.00 183.00 137.00 186.00 151.00 200.00 171.00 161.00 SPILLED GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92826 141.00 157.00 169.00 167.00 185.00 157.00 144.00 160.00 173.00 164.00 195.00 SPILLED 162.00 164.00 186.00 169.00 206.00 174.00 159.00 167.00 179.00 176.00 211.00 171.00 167.00 174.00 184.00 181.00 224.00 172.00 172.00 181.00 175.00 185.00 226.00 171.00 170.00 176.00 171.00 188.00 222.00 SPILLED 165.00 170.00 168.00 179.00 222.00 SPILLED 160.00 167.00 171.00 192.00 216.00 181.00 166.00 164.00 174.00 203.00 SPILLED 189.00 1356 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 157.00 167.00 174.00 165.00 187.00 148.00 162.00 157.00 164.00 175.00 168.00 171.00 200.00 158.00 145.00 193.00 190.00 150.00 SPILLED 170.00 175.00 143.00 176.00 169.00 153.00 152.00 164.00 189.00 176.00 167.00 170.00 161.00 171.00 170.00 170.00 196.00 159.00 166.00 168.00 155.00 183.00 182.00 173.00 218.00 155.00 162.00 197.00 200.00 148.00 SPILLED 171.00 198.00 144.00 186.00 172.00 167.00 164.00 170.00 202.00 SPILLED 164.00 181.00 175.00 184.00 187.00 175.00 202.00 166.00 182.00 183.00 154.00 194.00 196.00 181.00 SPILLED 158.00 164.00 203.00 212.00 156.00 183.00 177.00 214.00 155.00 191.00 177.00 171.00 183.00 173.00 212.00 SPILLED 176.00 188.00 178.00 181.00 189.00 189.00 191.00 166.00 180.00 182.00 158.00 195.00 201.00 186.00 245.00 165.00 176.00 203.00 205.00 156.00 189.00 185.00 212.00 160.00 190.00 180.00 179.00 199.00 177.00 SPILLED SPILLED 171.00 195.00 187.00 192.00 205.00 191.00 192.00 170.00 183.00 181.00 159.00 199.00 203.00 187.00 253.00 160.00 161.00 193.00 206.00 153.00 192.00 187.00 209.00 160.00 198.00 180.00 181.00 180.00 176.00 201.00 183.00 176.00 190.00 186.00 191.00 208.00 183.00 190.00 165.00 175.00 180.00 167.00 191.00 196.00 183.00 242.00 160.00 164.00 188.00 200.00 153.00 190.00 194.00 209.00 162.00 197.00 SPILLED 176.00 172.00 177.00 193.00 SPILLED 172.00 193.00 189.00 191.00 204.00 169.00 195.00 SPILLED SPILLED 179.00 159.00 189.00 194.00 183.00 236.00 156.00 159.00 194.00 195.00 157.00 187.00 197.00 209.00 162.00 202.00 189.00 180.00 185.00 173.00 194.00 128.00 170.00 190.00 191.00 191.00 205.00 186.00 201.00 SPILLED SPILLED 175.00 159.00 118.00 187.00 183.00 SPILLED 151.00 160.00 189.00 192.00 154.00 181.00 196.00 215.00 163.00 SPILLED 184.00 179.00 194.00 181.00 195.00 SPILLED 170.00 195.00 180.00 192.00 203.00 188.00 192.00 SPILLED SPILLED 181.00 163.00 176.00 183.00 184.00 235.00 156.00 158.00 187.00 197.00 152.00 SPILLED 191.00 209.00 165.00 SPILLED SPILLED 174.00 SPILLED 174.00 181.00 SPILLED 169.00 180.00 188.00 191.00 209.00 196.00 197.00 153.00 182.00 179.00 161.00 215.00 186.00 180.00 237.00 155.00 158.00 187.00 198.00 147.00 191.00 198.00 216.00 170.00 210.00 188.00 176.00 193.00 167.00 189.00 185.00 170.00 182.00 1357 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92858 C92859 C92860 148.00 160.00 160.00 152.00 173.00 167.00 186.00 188.00 184.00 187.00 186.00 183.00 198.00 198.00 178.00 187.00 199.00 181.00 194.00 202.00 178.00 190.00 191.00 174.00 188.00 SPILLED 182.00 189.00 SPILLED 176.00 GROUP: FEMALE 1 - 0 PPM C92861 C92862 C92863 C92864 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92876 C92877 C92878 C92879 C92880 C92881 C92882 C92883 C92884 C92885 140.00 111.00 122.00 SPILLED 151.00 124.00 130.00 126.00 123.00 125.00 123.00 110.00 116.00 136.00 114.00 109.00 117.00 SPILLED 130.00 CNTMD SPILLED 118.00 140.00 137.00 140.00 151.00 121.00 136.00 160.00 146.00 136.00 145.00 152.00 SPILLED 143.00 138.00 115.00 125.00 141.00 127.00 121.00 132.00 151.00 144.00 124.00 143.00 129.00 157.00 144.00 151.00 155.00 128.00 127.00 SPILLED 155.00 135.00 150.00 157.00 SPILLED 146.00 TECH SPILL 113.00 141.00 162.00 123.00 130.00 140.00 SPILLED 150.00 123.00 151.00 126.00 173.00 147.00 160.00 NOT TAKEN 120.00 128.00 NOT TAKEN 149.00 123.00 145.00 154.00 SPILLED 124.00 140.00 116.00 125.00 SPILLED 130.00 SPILLED SPILLED SPILLED 135.00 122.00 129.00 SPILLED SPILLED 133.00 154.00 133.00 132.00 149.00 126.00 156.00 167.00 SPILLED 136.00 140.00 119.00 125.00 144.00 124.00 126.00 SPILLED SPILLED 145.00 135.00 146.00 128.00 173.00 SPILLED 163.00 117.00 120.00 143.00 124.00 129.00 SPILLED SPILLED 126.00 137.00 107.00 118.00 SPILLED SPILLED 118.00 SPILLED SPILLED 146.00 125.00 139.00 115.00 SPILLED 125.00 149.00 116.00 131.00 144.00 122.00 141.00 130.00 SPILLED 148.00 131.00 105.00 129.00 135.00 SPILLED 120.00 SPILLED SPILLED 147.00 131.00 149.00 110.00 SPILLED 141.00 145.00 120.00 135.00 136.00 125.00 138.00 147.00 SPILLED 137.00 141.00 96.00 125.00 SPILLED SPILLED 117.00 SPILLED SPILLED 152.00 147.00 142.00 114.00 156.00 SPILLED 146.00 119.00 129.00 148.00 129.00 132.00 147.00 SPILLED 127.00 130.00 99.00 SPILLED 119.00 128.00 115.00 SPILLED SPILLED 154.00 131.00 145.00 117.00 161.00 142.00 141.00 126.00 SPILLED 146.00 129.00 142.00 151.00 SPILLED 139.00 132.00 106.00 136.00 124.00 130.00 120.00 SPILLED SPILLED 159.00 140.00 156.00 115.00 167.00 146.00 153.00 1358 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92886 C92887 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92898 C92899 C92900 C92901 C92902 C92903 C92904 C92905 C92906 C92907 C92908 C92909 C92910 C92911 C92912 C92913 C92914 C92915 C92916 142.00 133.00 142.00 117.00 SPILLED SPILLED 130.00 127.00 118.00 132.00 128.00 122.00 129.00 137.00 SPILLED 109.00 155.00 152.00 119.00 113.00 SPILLED SPILLED 123.00 117.00 SPILLED SPILLED 133.00 130.00 119.00 SPILLED 140.00 133.00 154.00 172.00 131.00 164.00 SPILLED 143.00 128.00 130.00 141.00 150.00 124.00 141.00 146.00 SPILLED 121.00 167.00 151.00 128.00 120.00 SPILLED 121.00 133.00 129.00 190.00 115.00 143.00 142.00 127.00 143.00 SPILLED SPILLED 158.00 SPILLED 132.00 169.00 SPILLED 153.00 SPILLED 128.00 153.00 SPILLED 137.00 147.00 164.00 SPILLED 131.00 175.00 155.00 133.00 128.00 139.00 123.00 132.00 SPILLED SPILLED 111.00 142.00 146.00 129.00 143.00 172.00 139.00 150.00 163.00 SPILLED 145.00 136.00 155.00 128.00 124.00 150.00 SPILLED 125.00 SPILLED 158.00 SPILLED SPILLED 175.00 158.00 126.00 128.00 SPILLED 134.00 135.00 136.00 SPILLED SPILLED 138.00 130.00 NOT TAKEN 162.00 169.00 SPILLED 161.00 SPILLED 136.00 162.00 138.00 165.00 138.00 125.00 SPILLED 183.00 132.00 149.00 173.00 SPILLED SPILLED 185.00 150.00 143.00 132.00 133.00 124.00 138.00 142.00 SPILLED 111.00 SPILLED 140.00 SPILLED 173.00 120.00 133.00 189.00 SPILLED 150.00 SPILLED 154.00 SPILLED 124.00 142.00 SPILLED 127.00 SPILLED 155.00 SPILLED 143.00 171.00 148.00 SPILLED 123.00 152.00 127.00 129.00 139.00 SPILLED 109.00 SPILLED 137.00 SPILLED 159.00 112.00 106.00 161.00 135.00 141.00 SPILLED 162.00 SPILLED 118.00 152.00 SPILLED 134.00 150.00 172.00 150.00 127.00 188.00 141.00 132.00 135.00 116.00 120.00 133.00 139.00 SPILLED 116.00 144.00 137.00 148.00 158.00 150.00 122.00 177.00 124.00 141.00 SPILLED 142.00 SPILLED 135.00 140.00 SPILLED 133.00 149.00 155.00 SPILLED SPILLED 190.00 SPILLED 130.00 129.00 SPILLED 116.00 135.00 134.00 SPILLED 104.00 135.00 137.00 SPILLED 164.00 133.00 117.00 149.00 122.00 156.00 140.00 150.00 SPILLED 125.00 143.00 143.00 137.00 159.00 171.00 SPILLED 122.00 180.00 181.00 146.00 133.00 SPILLED 125.00 132.00 134.00 200.00 118.00 146.00 134.00 SPILLED 126.00 SPILLED 132.00 147.00 123.00 SPILLED 130.00 149.00 129.00 134.00 155.00 SPILLED 141.00 147.00 163.00 157.00 131.00 173.00 172.00 138.00 128.00 114.00 SPILLED 132.00 129.00 SPILLED 112.00 130.00 128.00 147.00 152.00 1359 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92917 C92918 C92919 C92920 C92921 C92922 C92923 C92924 C92925 C92926 C92927 C92928 C92929 C92930 148.00 119.00 SPILLED SPILLED 127.00 136.00 SPILLED 144.00 134.00 143.00 120.00 130.00 114.00 147.00 152.00 121.00 SPILLED 120.00 126.00 156.00 125.00 SPILLED 145.00 SPILLED 190.00 138.00 133.00 179.00 SPILLED CNTMD 185.00 131.00 128.00 156.00 SPILLED SPILLED 155.00 152.00 138.00 143.00 132.00 178.00 161.00 115.00 NOT TAKEN SPILLED 130.00 135.00 SPILLED SPILLED 147.00 NOT TAKEN 141.00 157.00 129.00 181.00 172.00 133.00 SPILLED 134.00 153.00 SPILLED SPILLED 165.00 140.00 157.00 139.00 189.00 177.00 127.00 SPILLED 142.00 150.00 SPILLED SPILLED 156.00 145.00 135.00 139.00 182.00 158.00 123.00 143.00 148.00 153.00 SPILLED SPILLED 147.00 140.00 138.00 131.00 171.00 180.00 121.00 SPILLED 138.00 146.00 SPILLED SPILLED SPILLED 122.00 152.00 125.00 165.00 SPILLED 120.00 136.00 144.00 157.00 141.00 178.00 142.00 SPILLED 134.00 134.00 156.00 163.00 124.00 196.00 137.00 SPILLED SPILLED SPILLED 161.00 169.00 139.00 126.00 173.00 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92941 C92942 C92943 C92944 127.00 SPILLED 116.00 117.00 124.00 113.00 116.00 117.00 104.00 114.00 135.00 131.00 132.00 133.00 141.00 SPILLED 128.00 130.00 132.00 125.00 130.00 SPILLED 111.00 126.00 SPILLED SPILLED 137.00 SPILLED 148.00 SPILLED 129.00 135.00 148.00 135.00 134.00 SPILLED 144.00 140.00 SPILLED SPILLED 155.00 155.00 146.00 SPILLED 145.00 SPILLED 148.00 120.00 133.00 SPILLED 119.00 154.00 NOT TAKEN SPILLED 150.00 167.00 149.00 SPILLED 145.00 132.00 144.00 129.00 136.00 SPILLED 136.00 148.00 SPILLED 161.00 175.00 148.00 SPILLED SPILLED SPILLED 129.00 121.00 137.00 SPILLED SPILLED SPILLED SPILLED SPILLED 164.00 145.00 SPILLED 151.00 154.00 135.00 121.00 141.00 SPILLED SPILLED SPILLED 162.00 157.00 163.00 152.00 SPILLED 157.00 129.00 140.00 119.00 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 174.00 143.00 SPILLED 166.00 125.00 135.00 119.00 140.00 SPILLED 127.00 SPILLED 151.00 148.00 167.00 140.00 SPILLED 170.00 SPILLED 133.00 111.00 SPILLED SPILLED 120.00 SPILLED SPILLED 154.00 170.00 1360 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 C92970 C92971 C92972 C92973 C92974 C92975 117.00 98.00 119.00 112.00 SPILLED 127.00 109.00 103.00 113.00 114.00 132.00 123.00 SPILLED 102.00 139.00 SPILLED SPILLED 113.00 102.00 121.00 130.00 107.00 125.00 133.00 107.00 132.00 99.00 113.00 105.00 112.00 127.00 139.00 112.00 128.00 142.00 SPILLED 125.00 120.00 110.00 117.00 132.00 136.00 134.00 130.00 119.00 157.00 183.00 SPILLED 130.00 112.00 186.00 168.00 111.00 136.00 SPILLED 126.00 139.00 112.00 138.00 129.00 115.00 140.00 144.00 116.00 139.00 141.00 202.00 145.00 SPILLED 125.00 SPILLED 130.00 151.00 153.00 138.00 110.00 163.00 SPILLED SPILLED 133.00 115.00 148.00 SPILLED 110.00 148.00 159.00 SPILLED 155.00 123.00 139.00 126.00 123.00 146.00 NOT TAKEN 109.00 142.00 135.00 188.00 NOT TAKEN 114.00 123.00 115.00 135.00 SPILLED 144.00 122.00 123.00 126.00 SPILLED 157.00 114.00 115.00 SPILLED SPILLED 114.00 153.00 SPILLED NOT TAKEN 160.00 117.00 142.00 127.00 120.00 131.00 113.00 155.00 162.00 196.00 133.00 132.00 134.00 151.00 SPILLED 143.00 131.00 127.00 169.00 182.00 160.00 125.00 116.00 SPILLED 150.00 122.00 148.00 161.00 159.00 120.00 144.00 144.00 123.00 155.00 121.00 147.00 139.00 211.00 114.00 118.00 130.00 135.00 SPILLED 144.00 SPILLED 114.00 151.00 SPILLED 149.00 117.00 110.00 SPILLED SPILLED 111.00 164.00 SPILLED 146.00 110.00 139.00 134.00 119.00 142.00 116.00 140.00 151.00 192.00 121.00 127.00 125.00 139.00 143.00 143.00 139.00 117.00 157.00 161.00 153.00 116.00 112.00 SPILLED 144.00 118.00 138.00 144.00 153.00 119.00 136.00 163.00 122.00 153.00 113.00 149.00 162.00 SPILLED 126.00 128.00 SPILLED 136.00 143.00 137.00 SPILLED 121.00 165.00 SPILLED 145.00 124.00 121.00 SPILLED SPILLED 116.00 SPILLED 138.00 161.00 107.00 146.00 138.00 116.00 133.00 115.00 139.00 144.00 174.00 126.00 121.00 121.00 130.00 147.00 136.00 SPILLED 114.00 169.00 143.00 141.00 117.00 122.00 119.00 131.00 115.00 130.00 151.00 154.00 112.00 133.00 SPILLED 112.00 139.00 113.00 137.00 130.00 178.00 112.00 131.00 127.00 135.00 131.00 138.00 140.00 106.00 146.00 139.00 159.00 115.00 SPILLED SPILLED 131.00 114.00 129.00 142.00 147.00 110.00 140.00 128.00 117.00 140.00 1361 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 124.00 116.00 139.00 114.00 113.00 131.00 122.00 125.00 123.00 141.00 116.00 124.00 106.00 153.00 137.00 141.00 120.00 156.00 118.00 129.00 137.00 SPILLED SPILLED 140.00 145.00 134.00 144.00 131.00 SPILLED SPILLED 155.00 131.00 172.00 127.00 138.00 152.00 SPILLED 131.00 152.00 158.00 142.00 141.00 128.00 165.00 165.00 154.00 133.00 163.00 134.00 141.00 SPILLED NOT TAKEN 145.00 158.00 158.00 134.00 136.00 135.00 TECH SPILL 149.00 162.00 155.00 179.00 134.00 152.00 132.00 SPILLED 147.00 155.00 139.00 136.00 147.00 SPILLED 144.00 135.00 151.00 168.00 132.00 153.00 SPILLED 166.00 151.00 162.00 140.00 146.00 143.00 213.00 162.00 138.00 149.00 177.00 132.00 140.00 138.00 167.00 147.00 162.00 136.00 131.00 134.00 SPILLED 152.00 139.00 140.00 162.00 122.00 147.00 129.00 SPILLED 151.00 148.00 129.00 138.00 134.00 SPILLED SPILLED 137.00 145.00 151.00 124.00 130.00 126.00 SPILLED 138.00 132.00 132.00 126.00 131.00 SPILLED 142.00 136.00 140.00 160.00 126.00 140.00 131.00 141.00 133.00 138.00 131.00 SPILLED 141.00 SPILLED 141.00 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 C92998 C92999 C93000 C93001 C93002 C93003 SPILLED 133.00 124.00 114.00 134.00 120.00 117.00 124.00 SPILLED SPILLED 117.00 123.00 174.00 SPILLED 124.00 127.00 110.00 134.00 127.00 119.00 138.00 SPILLED SPILLED 123.00 127.00 SPILLED 153.00 126.00 133.00 114.00 147.00 131.00 123.00 155.00 SPILLED 159.00 118.00 133.00 132.00 SPILLED SPILLED 119.00 124.00 162.00 NOT TAKEN 128.00 137.00 SPILLED SPILLED 140.00 117.00 152.00 SPILLED SPILLED 146.00 121.00 135.00 124.00 153.00 SPILLED 151.00 SPILLED 133.00 160.00 SPILLED SPILLED 146.00 123.00 140.00 115.00 138.00 SPILLED SPILLED SPILLED 122.00 138.00 128.00 123.00 132.00 113.00 137.00 121.00 145.00 SPILLED 132.00 118.00 108.00 140.00 120.00 SPILLED 138.00 124.00 141.00 130.00 143.00 SPILLED SPILLED 129.00 130.00 SPILLED 118.00 123.00 138.00 121.00 NOT TAKEN 121.00 140.00 SPILLED SPILLED 134.00 109.00 143.00 117.00 125.00 132.00 116.00 SPILLED 145.00 SPILLED SPILLED 138.00 123.00 128.00 1362 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 C93029 C93030 C93031 C93032 C93033 C93034 127.00 132.00 109.00 SPILLED 141.00 127.00 141.00 135.00 SPILLED SPILLED SPILLED 130.00 80.00 110.00 125.00 127.00 125.00 121.00 120.00 91.00 129.00 137.00 106.00 112.00 110.00 111.00 SPILLED 118.00 117.00 SPILLED 124.00 129.00 129.00 118.00 SPILLED 153.00 117.00 141.00 131.00 SPILLED 116.00 162.00 139.00 99.00 124.00 132.00 133.00 146.00 131.00 143.00 109.00 131.00 141.00 119.00 121.00 122.00 128.00 121.00 124.00 119.00 SPILLED 131.00 168.00 128.00 109.00 SPILLED 156.00 179.00 153.00 139.00 142.00 117.00 SPILLED 148.00 123.00 120.00 141.00 SPILLED 157.00 139.00 131.00 115.00 145.00 165.00 123.00 129.00 125.00 123.00 131.00 146.00 134.00 SPILLED 150.00 131.00 137.00 NOT TAKEN SPILLED NOT TAKEN 129.00 153.00 140.00 164.00 SPILLED 194.00 SPILLED 138.00 140.00 SPILLED SPILLED 141.00 SPILLED 148.00 111.00 148.00 151.00 134.00 136.00 126.00 SPILLED 139.00 NOT TAKEN 119.00 SPILLED 124.00 131.00 134.00 141.00 133.00 146.00 138.00 160.00 SPILLED 177.00 140.00 129.00 137.00 144.00 SPILLED 143.00 141.00 151.00 120.00 142.00 139.00 144.00 142.00 SPILLED SPILLED 131.00 133.00 SPILLED 148.00 133.00 129.00 SPILLED SPILLED 147.00 SPILLED 143.00 SPILLED 164.00 SPILLED 121.00 150.00 140.00 SPILLED SPILLED 140.00 135.00 SPILLED 141.00 162.00 135.00 151.00 SPILLED SPILLED 129.00 125.00 SPILLED 135.00 133.00 120.00 SPILLED 127.00 138.00 119.00 155.00 119.00 SPILLED 144.00 121.00 136.00 131.00 SPILLED 147.00 136.00 140.00 SPILLED 136.00 147.00 130.00 135.00 133.00 SPILLED 116.00 121.00 SPILLED 118.00 128.00 131.00 SPILLED 132.00 139.00 128.00 139.00 119.00 139.00 SPILLED 116.00 139.00 131.00 152.00 SPILLED 135.00 136.00 117.00 135.00 159.00 130.00 134.00 129.00 SPILLED 128.00 126.00 160.00 125.00 124.00 111.00 SPILLED 135.00 136.00 123.00 141.00 104.00 136.00 SPILLED 117.00 140.00 126.00 SPILLED 134.00 124.00 131.00 SPILLED 125.00 SPILLED SPILLED 128.00 132.00 SPILLED 134.00 118.00 SPILLED 131.00 131.00 123.00 SPILLED SPILLED 139.00 118.00 135.00 120.00 146.00 157.00 116.00 SPILLED 134.00 SPILLED SPILLED 138.00 141.00 SPILLED 132.00 155.00 143.00 125.00 134.00 SPILLED SPILLED 125.00 SPILLED 136.00 1363 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 SPILLED 111.00 119.00 130.00 119.00 SPILLED 132.00 128.00 118.00 123.00 SPILLED 142.00 147.00 118.00 116.00 118.00 143.00 134.00 126.00 117.00 136.00 SPILLED 146.00 130.00 127.00 SPILLED 222.00 156.00 154.00 121.00 125.00 129.00 186.00 SPILLED 134.00 144.00 155.00 173.00 161.00 143.00 131.00 SPILLED SPILLED SPILLED 148.00 119.00 123.00 148.00 152.00 125.00 141.00 SPILLED 144.00 SPILLED 150.00 159.00 131.00 136.00 NOT TAKEN 182.00 210.00 133.00 122.00 163.00 165.00 155.00 146.00 SPILLED 151.00 SPILLED SPILLED 164.00 148.00 128.00 153.00 187.00 127.00 129.00 157.00 70.00 129.00 144.00 SPILLED 149.00 SPILLED SPILLED 142.00 143.00 117.00 207.00 SPILLED 127.00 121.00 150.00 142.00 129.00 137.00 121.00 143.00 SPILLED 151.00 141.00 143.00 126.00 SPILLED 161.00 SPILLED 118.00 152.00 160.00 134.00 126.00 141.00 145.00 SPILLED 149.00 147.00 133.00 SPILLED SPILLED 152.00 125.00 SPILLED 153.00 SPILLED SPILLED 134.00 141.00 146.00 208.00 164.00 127.00 136.00 118.00 157.00 137.00 118.00 133.00 SPILLED 138.00 135.00 124.00 SPILLED 151.00 SPILLED 154.00 137.00 134.00 126.00 148.00 144.00 132.00 127.00 SPILLED GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 C93057 C93058 C93059 C93060 C93061 C93062 125.00 129.00 115.00 SPILLED 136.00 129.00 136.00 151.00 135.00 135.00 116.00 112.00 SPILLED SPILLED 116.00 SPILLED 130.00 SPILLED 124.00 153.00 136.00 160.00 123.00 117.00 152.00 142.00 128.00 126.00 139.00 133.00 141.00 159.00 148.00 148.00 131.00 124.00 SPILLED SPILLED 121.00 125.00 145.00 135.00 133.00 NOT TAKEN 138.00 135.00 123.00 132.00 SPILLED SPILLED 137.00 SPILLED 145.00 141.00 136.00 144.00 TECH SPILL 130.00 130.00 SPILLED SPILLED 119.00 SPILLED 135.00 134.00 128.00 132.00 135.00 131.00 123.00 129.00 SPILLED 136.00 SPILLED 130.00 133.00 127.00 139.00 146.00 129.00 129.00 140.00 SPILLED 141.00 SPILLED SPILLED 128.00 117.00 132.00 137.00 132.00 129.00 129.00 SPILLED 143.00 SPILLED 144.00 138.00 124.00 130.00 128.00 121.00 125.00 171.00 SPILLED 133.00 SPILLED SPILLED 130.00 131.00 133.00 122.00 SPILLED 123.00 1364 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 118.00 118.00 112.00 121.00 122.00 122.00 149.00 151.00 110.00 125.00 139.00 138.00 123.00 124.00 123.00 128.00 139.00 129.00 125.00 SPILLED 133.00 115.00 129.00 148.00 SPILLED 131.00 157.00 SPILLED 133.00 130.00 146.00 134.00 125.00 112.00 121.00 119.00 SPILLED 188.00 SPILLED 106.00 138.00 138.00 167.00 123.00 127.00 127.00 136.00 190.00 133.00 113.00 SPILLED 133.00 119.00 SPILLED SPILLED 144.00 126.00 150.00 SPILLED 135.00 129.00 136.00 128.00 131.00 120.00 138.00 149.00 138.00 172.00 168.00 115.00 145.00 146.00 180.00 136.00 160.00 140.00 136.00 143.00 136.00 124.00 SPILLED 140.00 126.00 141.00 SPILLED 159.00 133.00 156.00 SPILLED 132.00 143.00 140.00 SPILLED 126.00 125.00 124.00 NOT TAKEN 124.00 161.00 NOT TAKEN 110.00 SPILLED 143.00 138.00 NOT TAKEN 139.00 132.00 142.00 167.00 130.00 116.00 SPILLED 134.00 NOT TAKEN 133.00 141.00 120.00 130.00 204.00 SPILLED 151.00 SPILLED 143.00 139.00 126.00 119.00 SPILLED 136.00 159.00 106.00 SPILLED 155.00 SPILLED 156.00 131.00 151.00 154.00 152.00 123.00 SPILLED 168.00 163.00 151.00 SPILLED 132.00 159.00 SPILLED 128.00 134.00 154.00 122.00 119.00 116.00 SPILLED 122.00 143.00 105.00 SPILLED 138.00 165.00 SPILLED 129.00 146.00 147.00 128.00 126.00 SPILLED 142.00 133.00 130.00 123.00 118.00 SPILLED SPILLED 136.00 139.00 SPILLED 118.00 126.00 117.00 SPILLED 122.00 143.00 114.00 SPILLED 146.00 149.00 146.00 138.00 141.00 161.00 120.00 120.00 156.00 144.00 SPILLED 137.00 125.00 129.00 SPILLED 123.00 133.00 134.00 158.00 115.00 132.00 119.00 SPILLED 128.00 144.00 113.00 SPILLED 142.00 147.00 138.00 132.00 143.00 193.00 122.00 127.00 SPILLED 142.00 124.00 SPILLED SPILLED 116.00 156.00 122.00 121.00 127.00 156.00 114.00 131.00 SPILLED 135.00 129.00 151.00 114.00 SPILLED 144.00 150.00 140.00 125.00 142.00 140.00 124.00 117.00 SPILLED 152.00 129.00 SPILLED 109.00 119.00 143.00 111.00 134.00 130.00 166.00 126.00 128.00 119.00 127.00 128.00 137.00 115.00 154.00 134.00 160.00 146.00 126.00 148.00 148.00 111.00 135.00 143.00 135.00 SPILLED 137.30 SPILLED 135.00 155.00 123.00 135.00 135.00 158.00 1365 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 134.00 134.00 146.00 109.00 128.00 123.00 156.00 111.00 152.00 126.00 141.00 171.00 119.00 121.00 121.00 121.00 SPILLED 161.00 128.00 SPILLED 114.00 SPILLED 132.00 162.00 SPILLED 159.00 142.00 155.00 153.00 124.00 125.00 127.00 125.00 180.00 142.00 138.00 177.00 120.00 146.00 138.00 158.00 121.00 159.00 139.00 162.00 154.00 124.00 SPILLED 122.00 129.00 SPILLED 191.00 141.00 SPILLED 132.00 SPILLED 131.00 162.00 122.00 155.00 128.00 166.00 143.00 124.00 136.00 122.00 132.00 SPILLED 135.00 155.00 181.00 132.00 127.00 129.00 150.00 124.00 170.00 151.00 184.00 170.00 130.00 136.00 124.00 138.00 207.00 171.00 SPILLED 171.00 128.00 128.00 120.00 SPILLED 129.00 156.00 139.00 SPILLED 133.00 125.00 135.00 118.00 126.00 199.00 137.00 SPILLED 169.00 136.00 121.00 119.00 148.00 SPILLED 158.00 124.00 204.00 140.00 126.00 138.00 127.00 132.00 SPILLED 128.00 SPILLED 161.00 SPILLED SPILLED 127.00 144.00 125.00 165.00 143.00 SPILLED 139.00 117.00 132.00 125.00 133.00 SPILLED 135.00 124.00 158.00 SPILLED SPILLED 116.00 150.00 123.00 157.00 138.00 208.00 132.00 117.00 122.00 128.00 126.00 SPILLED 137.00 127.00 168.00 SPILLED 136.00 115.00 151.00 119.00 SPILLED 128.00 SPILLED 153.00 129.00 127.00 136.00 130.00 SPILLED GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 C93116 C93117 C93118 C93119 C93120 C93121 137.00 137.00 SPILLED 126.00 108.00 118.00 113.00 108.00 118.00 131.00 SPILLED 152.00 142.00 SPILLED 140.00 118.00 121.00 117.00 121.00 112.00 133.00 105.00 159.00 137.00 SPILLED 135.00 118.00 124.00 SPILLED 124.00 113.00 128.00 106.00 149.00 132.00 119.00 NOT TAKEN 112.00 126.00 122.00 126.00 116.00 122.00 97.00 145.00 135.00 120.00 130.00 SPILLED SPILLED 128.00 116.00 SPILLED SPILLED 136.00 129.00 110.00 109.00 121.00 SPILLED 110.00 106.00 124.00 SPILLED 141.00 125.00 119.00 111.00 SPILLED 121.00 123.00 106.00 120.00 108.00 136.00 119.00 124.00 114.00 SPILLED 122.00 113.00 96.00 122.00 99.00 128.00 111.00 120.00 107.00 116.00 SPILLED 118.00 101.00 107.00 98.00 SPILLED 124.00 129.00 115.00 122.00 123.00 119.00 107.00 124.00 103.00 1366 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 C93147 C93148 C93149 C93150 C93151 C93152 134.00 127.00 SPILLED 104.00 103.00 125.00 106.00 128.00 128.00 133.00 130.00 129.00 139.00 136.00 125.00 124.00 117.00 133.00 130.00 130.00 149.00 132.00 SPILLED 110.00 115.00 118.00 126.00 113.00 160.00 126.00 133.00 151.00 150.00 SPILLED 118.00 104.00 122.00 126.00 136.00 137.00 131.00 129.00 124.00 126.00 132.00 118.00 143.00 122.00 137.00 140.00 SPILLED 135.00 145.00 122.00 110.00 105.00 124.00 126.00 SPILLED 171.00 123.00 146.00 SPILLED 131.00 151.00 120.00 SPILLED 123.00 114.00 146.00 133.00 135.00 129.00 124.00 124.00 140.00 111.00 141.00 121.00 SPILLED 153.00 162.00 142.00 SPILLED 125.00 SPILLED 105.00 122.00 118.00 134.00 160.00 122.00 SPILLED 134.00 NOT TAKEN SPILLED 119.00 103.00 134.00 121.00 147.00 149.00 127.00 120.00 118.00 128.00 137.00 116.00 120.00 121.00 124.00 138.00 SPILLED NOT TAKEN SPILLED NOT TAKEN 128.00 106.00 128.00 116.00 133.00 159.00 111.00 128.00 157.00 SPILLED 122.00 108.00 125.00 119.00 146.00 144.00 131.00 123.00 120.00 132.00 147.00 121.00 136.00 126.00 134.00 152.00 182.00 153.00 SPILLED SPILLED 132.00 126.00 154.00 163.00 129.00 126.00 124.00 SPILLED 117.00 101.00 108.00 113.00 139.00 124.00 130.00 112.00 105.00 134.00 129.00 115.00 128.00 117.00 SPILLED 138.00 139.00 SPILLED 130.00 SPILLED 129.00 129.00 SPILLED 142.00 122.00 136.00 125.00 127.00 SPILLED 133.00 126.00 122.00 134.00 141.00 122.00 116.00 SPILLED 125.00 134.00 113.00 132.00 123.00 117.00 136.00 SPILLED 158.00 123.00 125.00 128.00 147.00 SPILLED 139.00 124.00 SPILLED SPILLED 109.00 124.00 101.00 128.00 115.00 131.00 138.00 124.00 106.00 113.00 128.00 131.00 120.00 121.00 119.00 111.00 145.00 166.00 SPILLED SPILLED 105.00 118.00 135.00 150.00 147.00 123.00 129.00 138.00 118.00 104.00 100.00 111.00 120.00 134.00 131.00 124.00 115.00 112.00 117.00 131.00 114.00 114.00 122.00 116.00 126.00 SPILLED 137.00 123.00 SPILLED 119.00 136.00 155.00 152.00 133.00 128.00 130.00 138.00 122.00 102.00 121.00 116.00 125.00 133.00 117.00 126.00 116.00 127.00 127.00 126.00 126.00 122.00 125.00 138.00 145.00 SPILLED 115.00 118.00 116.00 132.00 SPILLED 149.00 123.00 123.00 1367 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 C93178 C93179 C93180 SPILLED 130.00 131.00 115.00 130.00 124.00 118.00 140.00 119.00 149.00 111.00 133.00 124.00 129.00 110.00 129.00 122.00 122.00 SPILLED 120.00 118.00 119.00 118.00 112.00 118.00 SPILLED 115.00 SPILLED SPILLED 136.00 145.00 117.00 123.00 128.00 114.00 148.00 SPILLED 159.00 121.00 125.00 129.00 132.00 135.00 149.00 127.00 130.00 SPILLED 116.00 133.00 SPILLED 133.00 117.00 117.00 127.00 116.00 153.00 152.00 130.00 152.00 118.00 145.00 122.00 118.00 151.00 SPILLED 169.00 SPILLED 109.00 128.00 135.00 133.00 136.00 126.00 137.00 SPILLED 119.00 142.00 SPILLED 127.00 124.00 118.00 134.00 115.00 SPILLED SPILLED 128.00 115.00 109.00 126.00 118.00 NOT TAKEN 148.00 136.00 141.00 118.00 SPILLED 122.00 145.00 122.00 139.00 137.00 132.00 SPILLED SPILLED 109.00 125.00 114.00 122.00 115.00 137.00 114.00 138.00 SPILLED 137.00 131.00 118.00 137.00 137.00 161.00 138.00 146.00 120.00 SPILLED 135.00 142.00 138.00 146.00 134.00 153.00 SPILLED 123.00 125.00 135.00 127.00 129.00 118.00 126.00 120.00 148.00 SPILLED 129.00 128.00 111.00 134.00 116.00 146.00 SPILLED 149.00 120.00 118.00 126.00 140.00 122.00 141.00 120.00 133.00 SPILLED 119.00 116.00 123.00 111.00 116.00 111.00 121.00 114.00 137.00 SPILLED 129.00 147.00 118.00 134.00 111.00 147.00 SPILLED 138.00 115.00 116.00 121.00 SPILLED 120.00 132.00 123.00 130.00 SPILLED 130.00 SPILLED SPILLED 125.00 120.00 119.00 123.00 113.00 135.00 SPILLED 127.00 159.00 112.00 125.00 126.00 148.00 130.00 SPILLED 106.00 SPILLED 115.00 139.00 131.00 123.00 117.00 SPILLED SPILLED 115.00 SPILLED SPILLED 109.00 113.00 111.00 112.00 111.00 139.00 SPILLED 126.00 130.00 107.00 131.00 123.00 144.00 SPILLED 146.00 107.00 116.00 119.00 127.00 111.00 116.00 121.00 140.00 SPILLED SPILLED 130.00 SPILLED 118.00 113.00 134.00 118.00 113.00 SPILLED SPILLED 130.00 SPILLED 111.00 133.00 129.00 144.00 128.00 SPILLED 113.00 112.00 117.00 134.00 123.00 SPILLED 112.00 SPILLED SPILLED 128.00 SPILLED SPILLED 119.00 113.00 127.00 116.00 SPILLED 140.00 1368 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 C93206 C93207 C93208 C93209 C93210 C93211 124.00 119.00 129.00 115.00 SPILLED 114.00 126.00 140.00 SPILLED 138.00 132.00 115.00 126.00 130.00 152.00 112.00 106.00 128.00 127.00 133.00 SPILLED 100.00 130.00 143.00 137.00 127.00 122.00 122.00 121.00 141.00 101.00 122.00 124.00 121.00 108.00 164.00 117.00 132.00 129.00 SPILLED 124.00 151.00 111.00 137.00 133.00 SPILLED 134.00 102.00 127.00 118.00 125.00 121.00 121.00 130.00 138.00 SPILLED 112.00 128.00 118.00 123.00 128.00 SPILLED 126.00 132.00 133.00 124.00 SPILLED 123.00 119.00 140.00 SPILLED 130.00 129.00 109.00 138.00 152.00 143.00 SPILLED 103.00 131.00 126.00 132.00 130.00 120.00 120.00 147.00 160.00 114.00 123.00 128.00 SPILLED 125.00 116.00 119.00 123.00 127.00 127.00 SPILLED 115.00 140.00 113.00 SPILLED 122.00 143.00 129.00 132.00 157.00 129.00 SPILLED 105.00 140.00 118.00 143.00 119.00 124.00 141.00 140.00 131.00 105.00 118.00 112.00 SPILLED 161.00 116.00 135.00 126.00 132.00 129.00 SPILLED 112.00 122.00 130.00 SPILLED 135.00 142.00 121.00 133.00 153.00 131.00 SPILLED 102.00 138.00 121.00 152.00 122.00 127.00 128.00 144.00 135.00 126.00 125.00 120.00 SPILLED 138.00 117.00 132.00 107.00 136.00 117.00 143.00 119.00 125.00 120.00 120.00 126.00 138.00 114.00 131.00 146.00 125.00 SPILLED 101.00 126.00 119.00 125.00 109.00 SPILLED 144.00 134.00 130.00 SPILLED 110.00 100.00 SPILLED SPILLED 106.00 130.00 119.00 128.00 123.00 133.00 107.00 131.00 125.00 127.00 128.00 146.00 120.00 123.00 155.00 126.00 120.00 105.00 122.00 109.00 SPILLED 107.00 107.00 132.00 132.00 139.00 115.00 118.00 120.00 107.00 126.00 110.00 132.00 107.00 125.00 120.00 149.00 107.00 118.00 125.00 124.00 120.00 151.00 106.00 116.00 138.00 SPILLED 112.00 99.00 108.00 115.00 SPILLED SPILLED SPILLED SPILLED 128.00 128.00 SPILLED 124.00 109.00 SPILLED 127.00 112.00 129.00 120.00 126.00 113.00 137.00 109.00 123.00 123.00 125.00 127.00 134.00 101.00 105.00 150.00 156.00 118.00 106.00 120.00 111.00 201.00 117.00 SPILLED 132.00 125.00 130.00 SPILLED 115.00 104.00 120.00 127.00 124.00 131.00 112.00 140.00 114.00 149.00 120.00 117.00 123.00 125.00 132.00 151.00 111.00 126.00 176.00 126.00 113.00 108.00 124.00 113.00 SPILLED 111.00 SPILLED 122.00 135.00 135.00 127.00 114.00 108.00 SPILLED 121.00 122.00 1369 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 114.00 121.00 116.00 145.00 113.00 SPILLED 127.00 141.00 SPILLED 135.00 123.00 117.00 124.00 109.00 SPILLED 117.00 151.00 SPILLED 131.00 130.00 113.00 SPILLED 118.00 SPILLED 139.00 161.00 SPILLED 137.00 126.00 SPILLED 128.00 115.00 SPILLED 119.00 153.00 SPILLED 141.00 125.00 SPILLED 133.00 122.00 SPILLED 124.00 155.00 SPILLED 125.00 119.00 SPILLED 126.00 114.00 153.00 SPILLED SPILLED SPILLED 140.00 117.00 117.00 122.00 114.00 145.00 SPILLED SPILLED SPILLED 133.00 113.00 114.00 125.00 110.00 132.00 SPILLED 139.00 SPILLED 135.00 116.00 119.00 119.00 118.00 130.00 SPILLED 129.00 SPILLED 142.00 117.00 120.00 121.00 112.00 137.00 SPILLED SPILLED SPILLED 1370 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: C92501 C92502 C92503 C92504 C92505 C92507 C92508 C92509 C92510 C92511 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92521 C92522 C92523 C92525 C92527 C92528 C92530 C92531 C92532 C92533 C92534 C92535 C92536 MALE 1 - 0 PPM SPILLED 223.00 182.00 174.00 187.00 177.00 190.00 118.00 194.00 SPILLED 184.00 171.00 180.00 173.00 178.00 179.00 202.00 207.00 135.00 SPILLED 191.00 190.00 187.00 177.00 211.00 213.00 187.00 185.00 167.00 173.00 217.00 204.00 203.00 200.00 168.00 173.00 192.00 199.00 184.00 187.00 166.00 SPILLED 174.00 172.00 185.00 184.00 189.00 189.00 175.00 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 236.00 212.00 204.00 180.00 169.00 203.00 193.00 175.00 178.00 221.00 165.00 177.00 163.00 186.00 180.00 169.00 172.00 203.00 231.00 182.00 170.00 206.00 173.00 162.00 191.00 206.00 162.00 189.00 185.00 157.00 169.00 174.00 181.00 169.00 SPILLED SPILLED 234.00 199.00 165.00 187.00 188.00 222.00 174.00 175.00 165.00 190.00 175.00 153.00 NOT TAKEN 207.00 NOT TAKEN 183.00 174.00 220.00 181.00 161.00 197.00 205.00 165.00 192.00 NOT TAKEN 156.00 166.00 181.00 157.00 NOT TAKEN SPILLED 207.00 NOT TAKEN 194.00 175.00 181.00 186.00 217.00 181.00 178.00 173.00 194.00 174.00 177.00 194.00 196.00 176.00 218.00 190.00 160.00 203.00 206.00 168.00 205.00 160.00 175.00 187.00 187.00 SPILLED SPILLED 203.00 173.00 209.00 186.00 213.00 179.00 185.00 169.00 201.00 171.00 183.00 SPILLED 192.00 181.00 209.00 181.00 172.00 212.00 207.00 171.00 204.00 162.00 174.00 186.00 188.00 SPILLED SPILLED 201.00 172.00 199.00 185.00 214.00 179.00 165.00 187.00 191.00 168.00 187.00 192.00 199.00 184.00 226.00 178.00 164.00 216.00 232.00 163.00 196.00 162.00 188.00 186.00 188.00 SPILLED 219.00 195.00 173.00 190.00 196.00 194.00 161.00 169.00 188.00 172.00 162.00 170.00 173.00 189.00 182.00 200.00 166.00 163.00 204.00 212.00 153.00 182.00 140.00 181.00 181.00 174.00 SPILLED 212.00 186.00 190.00 173.00 175.00 197.00 157.00 173.00 162.00 174.00 160.00 180.00 170.00 184.00 179.00 199.00 160.00 165.00 210.00 208.00 152.00 175.00 152.00 176.00 169.00 175.00 SPILLED 198.00 194.00 181.00 174.00 186.00 188.00 168.00 170.00 159.00 179.00 158.00 169.00 188.00 169.00 171.00 188.00 153.00 178.00 190.00 203.00 156.00 174.00 158.00 167.00 166.00 170.00 SPILLED 169.00 178.00 171.00 187.00 169.00 1371 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92537 C92538 C92539 C92540 C92541 C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 C92563 C92564 C92565 C92566 C92567 C92568 212.00 193.00 218.00 210.00 198.00 180.00 170.00 211.00 201.00 201.00 177.00 163.00 205.00 165.00 193.00 148.00 162.00 205.00 176.00 202.00 203.00 181.00 185.00 182.00 193.00 SPILLED 193.00 209.00 193.00 179.00 198.00 219.00 203.00 211.00 208.00 203.00 184.00 172.00 208.00 184.00 199.00 176.00 162.00 204.00 172.00 193.00 149.00 157.00 215.00 182.00 SPILLED 202.00 178.00 184.00 187.00 SPILLED 225.00 203.00 200.00 195.00 175.00 195.00 212.00 204.00 215.00 198.00 190.00 184.00 166.00 190.00 182.00 196.00 172.00 157.00 208.00 163.00 189.00 143.00 151.00 215.00 171.00 195.00 200.00 175.00 191.00 174.00 SPILLED SPILLED 199.00 SPILLED 189.00 185.00 187.00 189.00 205.00 215.00 203.00 191.00 181.00 172.00 197.00 187.00 191.00 181.00 163.00 208.00 164.00 204.00 146.00 158.00 214.00 177.00 198.00 185.00 178.00 182.00 181.00 170.00 202.00 197.00 187.00 174.00 180.00 184.00 212.00 196.00 215.00 205.00 192.00 189.00 173.00 201.00 199.00 194.00 185.00 166.00 217.00 172.00 194.00 146.00 161.00 211.00 178.00 203.00 211.00 182.00 192.00 175.00 190.00 222.00 197.00 197.00 189.00 177.00 186.00 217.00 194.00 218.00 208.00 197.00 184.00 167.00 205.00 198.00 196.00 182.00 170.00 217.00 169.00 196.00 146.00 161.00 211.00 184.00 187.00 209.00 180.00 194.00 176.00 198.00 215.00 203.00 196.00 191.00 167.00 194.00 227.00 209.00 227.00 214.00 201.00 190.00 173.00 197.00 187.00 193.00 SPILLED 161.00 221.00 174.00 191.00 137.00 163.00 222.00 180.00 199.00 205.00 178.00 198.00 187.00 185.00 228.00 201.00 197.00 182.00 191.00 196.00 212.00 210.00 219.00 217.00 202.00 192.00 168.00 185.00 188.00 184.00 179.00 168.00 217.00 163.00 192.00 127.00 154.00 217.00 168.00 212.00 196.00 177.00 194.00 180.00 184.00 215.00 195.00 208.00 171.00 174.00 204.00 218.00 211.00 211.00 193.00 SPILLED 170.00 SPILLED 180.00 176.00 176.00 161.00 219.00 162.00 195.00 119.00 147.00 195.00 182.00 183.00 201.00 182.00 208.00 173.00 196.00 224.00 202.00 208.00 176.00 186.00 210.00 193.00 204.00 200.00 180.00 170.00 166.00 180.00 181.00 173.00 179.00 150.00 209.00 159.00 186.00 126.00 142.00 193.00 166.00 174.00 196.00 168.00 191.00 178.00 176.00 217.00 188.00 185.00 173.00 190.00 1372 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92569 C92570 177.00 199.00 181.00 184.00 168.00 192.00 176.00 194.00 193.00 188.00 194.00 195.00 195.00 198.00 183.00 192.00 175.00 177.00 168.00 174.00 GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 220.00 200.00 204.00 186.00 198.00 196.00 207.00 192.00 183.00 156.00 174.00 165.00 CNTMD 179.00 199.00 SPILLED 203.00 204.00 166.00 178.00 175.00 196.00 TECH SPILL 195.00 SPILLED 186.00 221.00 204.00 208.00 190.00 203.00 190.00 206.00 200.00 190.00 157.00 169.00 175.00 CNTMD 181.00 197.00 181.00 192.00 213.00 166.00 173.00 166.00 194.00 173.00 191.00 182.00 183.00 215.00 196.00 201.00 192.00 199.00 192.00 205.00 194.00 190.00 148.00 160.00 176.00 202.00 189.00 198.00 182.00 189.00 212.00 164.00 169.00 164.00 186.00 164.00 195.00 185.00 181.00 212.00 205.00 198.00 186.00 199.00 200.00 194.00 192.00 185.00 150.00 145.00 167.00 186.00 194.00 206.00 183.00 194.00 211.00 166.00 182.00 NOT TAKEN 185.00 169.00 190.00 179.00 159.00 217.00 200.00 207.00 190.00 195.00 192.00 200.00 190.00 184.00 156.00 181.00 179.00 CNTMD 184.00 205.00 197.00 193.00 214.00 165.00 175.00 195.00 182.00 189.00 191.00 176.00 199.00 199.00 207.00 198.00 209.00 196.00 195.00 193.00 183.00 158.00 170.00 167.00 191.00 197.00 213.00 194.00 190.00 206.00 171.00 181.00 195.00 178.00 192.00 199.00 182.00 195.00 208.00 202.00 213.00 217.00 206.00 208.00 206.00 195.00 160.00 167.00 170.00 212.00 220.00 219.00 SPILLED 196.00 199.00 182.00 184.00 195.00 166.00 203.00 192.00 198.00 179.00 193.00 199.00 181.00 205.00 197.00 197.00 179.00 184.00 150.00 167.00 162.00 211.00 195.00 219.00 185.00 193.00 204.00 174.00 186.00 187.00 164.00 202.00 190.00 179.00 173.00 196.00 202.00 194.00 197.00 189.00 192.00 181.00 178.00 170.00 164.00 173.00 200.00 193.00 207.00 172.00 189.00 SPILLED 178.00 181.00 185.00 170.00 197.00 202.00 192.00 188.00 194.00 202.00 193.00 197.00 188.00 186.00 181.00 186.00 167.00 164.00 174.00 184.00 203.00 196.00 170.00 183.00 182.00 174.00 177.00 177.00 159.00 193.00 182.00 180.00 1373 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92599 C92600 C92601 C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92611 C92612 C92614 C92615 C92616 C92617 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 216.00 177.00 160.00 170.00 197.00 187.00 197.00 196.00 207.00 SPILLED 176.00 170.00 185.00 SPILLED 190.00 175.00 208.00 177.00 153.00 SPILLED 186.00 159.00 182.00 217.00 197.00 SPILLED 214.00 SPILLED 237.00 211.00 170.00 164.00 177.00 200.00 188.00 199.00 197.00 203.00 227.00 173.00 173.00 178.00 SPILLED 193.00 168.00 218.00 172.00 162.00 211.00 185.00 163.00 169.00 213.00 192.00 SPILLED SPILLED 211.00 229.00 213.00 172.00 165.00 172.00 184.00 187.00 189.00 186.00 208.00 212.00 171.00 162.00 172.00 SPILLED 188.00 174.00 201.00 175.00 185.00 204.00 172.00 156.00 178.00 202.00 186.00 SPILLED SPILLED 206.00 232.00 215.00 NOT TAKEN 162.00 173.00 190.00 188.00 195.00 183.00 213.00 199.00 178.00 168.00 159.00 SPILLED 187.00 NOT TAKEN 208.00 178.00 175.00 NOT TAKEN 179.00 154.00 162.00 205.00 186.00 NOT TAKEN 222.00 191.00 230.00 210.00 166.00 174.00 178.00 189.00 194.00 182.00 205.00 125.00 180.00 171.00 184.00 SPILLED 191.00 213.00 182.00 164.00 179.00 161.00 179.00 215.00 180.00 SPILLED 210.00 235.00 210.00 176.00 168.00 195.00 196.00 202.00 195.00 208.00 170.00 184.00 164.00 184.00 SPILLED 190.00 209.00 171.00 170.00 191.00 172.00 184.00 214.00 152.00 212.00 217.00 239.00 216.00 178.00 178.00 198.00 187.00 207.00 212.00 217.00 193.00 167.00 189.00 SPILLED 189.00 213.00 173.00 167.00 183.00 168.00 180.00 202.00 188.00 221.00 230.00 SPILLED 208.00 180.00 165.00 193.00 198.00 187.00 208.00 208.00 193.00 168.00 122.00 SPILLED 196.00 212.00 159.00 160.00 190.00 160.00 178.00 205.00 195.00 211.00 201.00 231.00 215.00 178.00 173.00 195.00 191.00 173.00 180.00 201.00 198.00 154.00 175.00 SPILLED 187.00 207.00 160.00 160.00 179.00 158.00 183.00 195.00 164.00 213.00 213.00 SPILLED 225.00 176.00 165.00 196.00 185.00 180.00 199.00 201.00 194.00 165.00 183.00 SPILLED 182.00 201.00 163.00 162.00 177.00 153.00 187.00 185.00 SPILLED SPILLED 207.00 SPILLED 1374 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92647 C92648 C92649 C92651 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92662 C92663 C92664 178.00 203.00 173.00 184.00 SPILLED SPILLED 153.00 229.00 177.00 158.00 135.00 182.00 173.00 172.00 194.00 167.00 153.00 170.00 197.00 185.00 188.00 SPILLED 181.00 SPILLED 189.00 197.00 179.00 161.00 164.00 205.00 195.00 182.00 199.00 176.00 183.00 206.00 193.00 159.00 SPILLED 179.00 161.00 169.00 184.00 171.00 173.00 204.00 172.00 159.00 178.00 205.00 188.00 200.00 SPILLED 191.00 SPILLED 198.00 207.00 172.00 164.00 163.00 210.00 196.00 179.00 195.00 173.00 186.00 194.00 202.00 157.00 216.00 181.00 160.00 190.00 191.00 174.00 168.00 208.00 172.00 157.00 182.00 196.00 190.00 192.00 SPILLED 194.00 190.00 197.00 204.00 171.00 169.00 172.00 216.00 192.00 177.00 197.00 173.00 200.00 194.00 179.00 160.00 229.00 182.00 NOT TAKEN 199.00 179.00 176.00 154.00 NOT TAKEN 166.00 166.00 179.00 176.00 194.00 197.00 246.00 183.00 186.00 193.00 194.00 179.00 154.00 NOT TAKEN 217.00 195.00 177.00 238.00 171.00 205.00 189.00 SPILLED 164.00 214.00 182.00 199.00 184.00 168.00 180.00 177.00 170.00 182.00 198.00 187.00 196.00 SPILLED 190.00 192.00 196.00 218.00 173.00 189.00 212.00 194.00 188.00 205.00 182.00 183.00 191.00 206.00 163.00 230.00 189.00 212.00 186.00 SPILLED 178.00 180.00 170.00 179.00 202.00 183.00 190.00 SPILLED 190.00 187.00 189.00 208.00 176.00 187.00 214.00 200.00 182.00 209.00 193.00 201.00 191.00 202.00 169.00 227.00 183.00 206.00 175.00 169.00 172.00 176.00 174.00 190.00 189.00 199.00 180.00 237.00 186.00 201.00 201.00 209.00 203.00 178.00 214.00 209.00 180.00 220.00 186.00 198.00 201.00 189.00 165.00 244.00 198.00 206.00 174.00 179.00 168.00 176.00 167.00 172.00 202.00 186.00 190.00 239.00 180.00 190.00 180.00 195.00 198.00 180.00 224.00 195.00 181.00 202.00 182.00 182.00 189.00 187.00 159.00 227.00 186.00 165.00 179.00 171.00 164.00 169.00 165.00 169.00 191.00 189.00 182.00 238.00 178.00 179.00 184.00 200.00 177.00 177.00 210.00 179.00 169.00 196.00 177.00 177.00 190.00 191.00 162.00 228.00 173.00 176.00 173.00 169.00 177.00 175.00 161.00 171.00 193.00 193.00 182.00 229.00 175.00 182.00 186.00 198.00 SPILLED 166.00 208.00 193.00 1375 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 31 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92665 C92666 C92667 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 198.00 212.00 210.00 SPILLED 171.00 189.00 174.00 182.00 SPILLED 189.00 175.00 166.00 194.00 204.00 180.00 175.00 189.00 182.00 189.00 172.00 203.00 SPILLED 168.00 211.00 194.00 224.00 211.00 207.00 175.00 184.00 179.00 188.00 191.00 180.00 166.00 162.00 189.00 217.00 183.00 174.00 189.00 102.00 178.00 173.00 203.00 201.00 152.00 210.00 206.00 220.00 217.00 206.00 178.00 186.00 170.00 192.00 195.00 181.00 168.00 165.00 195.00 209.00 174.00 179.00 189.00 167.00 184.00 178.00 206.00 201.00 160.00 215.00 200.00 215.00 214.00 SPILLED 172.00 183.00 180.00 186.00 203.00 180.00 NOT TAKEN 169.00 191.00 203.00 179.00 158.00 190.00 NOT TAKEN 194.00 184.00 204.00 SPILLED 168.00 216.00 203.00 212.00 197.00 218.00 173.00 189.00 183.00 184.00 209.00 180.00 175.00 200.00 205.00 180.00 181.00 190.00 194.00 175.00 208.00 SPILLED 178.00 215.00 206.00 228.00 206.00 200.00 171.00 191.00 182.00 186.00 198.00 190.00 180.00 202.00 193.00 193.00 177.00 181.00 191.00 181.00 196.00 198.00 176.00 206.00 195.00 225.00 215.00 194.00 175.00 188.00 169.00 187.00 201.00 188.00 189.00 205.00 200.00 181.00 190.00 199.00 195.00 189.00 201.00 SPILLED 175.00 224.00 196.00 248.00 199.00 191.00 163.00 182.00 180.00 175.00 207.00 187.00 181.00 198.00 190.00 187.00 177.00 200.00 189.00 186.00 202.00 SPILLED 180.00 198.00 193.00 236.00 202.00 186.00 164.00 179.00 166.00 164.00 182.00 178.00 182.00 202.00 187.00 SPILLED 173.00 191.00 177.00 183.00 199.00 SPILLED 166.00 203.00 197.00 SPILLED 198.00 187.00 158.00 184.00 172.00 174.00 186.00 188.00 181.00 202.00 181.00 174.00 179.00 195.00 188.00 173.00 193.00 198.00 161.00 204.00 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 178.00 174.00 192.00 176.00 173.00 177.00 184.00 170.00 171.00 180.00 178.00 155.00 171.00 182.00 186.00 172.00 186.00 177.00 193.00 181.00 186.00 175.00 189.00 189.00 192.00 169.00 179.00 189.00 184.00 161.00 177.00 168.00 178.00 167.00 171.00 174.00 178.00 161.00 178.00 176.00 1376 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 32 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92716 C92717 C92719 C92720 C92721 C92722 C92723 C92724 C92725 C92726 C92727 182.00 196.00 213.00 192.00 166.00 150.00 174.00 166.00 214.00 189.00 192.00 172.00 160.00 174.00 177.00 194.00 169.00 202.00 197.00 208.00 193.00 187.00 180.00 SPILLED 161.00 203.00 SPILLED SPILLED 170.00 187.00 180.00 183.00 SPILLED 212.00 192.00 168.00 150.00 177.00 169.00 222.00 186.00 191.00 179.00 154.00 159.00 176.00 197.00 170.00 198.00 200.00 203.00 199.00 196.00 181.00 SPILLED 164.00 208.00 SPILLED SPILLED 164.00 182.00 194.00 177.00 192.00 212.00 197.00 165.00 153.00 179.00 163.00 215.00 181.00 184.00 175.00 154.00 178.00 177.00 186.00 172.00 204.00 193.00 206.00 197.00 189.00 184.00 SPILLED 168.00 202.00 SPILLED 248.00 164.00 181.00 194.00 178.00 182.00 SPILLED 198.00 167.00 151.00 179.00 165.00 216.00 189.00 192.00 176.00 154.00 175.00 172.00 188.00 146.00 222.00 NOT TAKEN NOT TAKEN 171.00 195.00 NOT TAKEN SPILLED 163.00 NOT TAKEN SPILLED SPILLED 167.00 160.00 174.00 180.00 188.00 199.00 200.00 166.00 151.00 174.00 167.00 210.00 185.00 198.00 181.00 159.00 182.00 184.00 191.00 177.00 224.00 SPILLED 198.00 SPILLED 170.00 SPILLED SPILLED 163.00 203.00 181.00 185.00 189.00 212.00 189.00 169.00 157.00 175.00 167.00 222.00 191.00 190.00 172.00 160.00 180.00 180.00 187.00 183.00 215.00 203.00 208.00 239.00 180.00 215.00 255.00 165.00 190.00 195.00 180.00 191.00 216.00 202.00 121.00 162.00 186.00 164.00 219.00 187.00 197.00 171.00 164.00 173.00 178.00 184.00 176.00 SPILLED 204.00 204.00 SPILLED 164.00 SPILLED 235.00 167.00 173.00 196.00 174.00 187.00 206.00 184.00 164.00 143.00 169.00 159.00 204.00 175.00 188.00 169.00 154.00 161.00 173.00 174.00 170.00 220.00 193.00 187.00 227.00 167.00 223.00 232.00 154.00 188.00 193.00 179.00 SPILLED 190.00 176.00 150.00 159.00 176.00 159.00 195.00 179.00 189.00 181.00 155.00 159.00 171.00 179.00 168.00 219.00 189.00 190.00 224.00 171.00 SPILLED SPILLED 151.00 181.00 178.00 170.00 155.00 196.00 183.00 173.00 SPILLED 172.00 169.00 189.00 171.00 178.00 188.00 163.00 160.00 160.00 167.00 170.00 215.00 195.00 194.00 216.00 162.00 SPILLED 216.00 153.00 170.00 189.00 1377 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 33 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92728 C92729 C92730 C92732 C92733 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92745 C92746 C92747 C92748 C92749 C92750 219.00 210.00 SPILLED 190.00 176.00 181.00 170.00 181.00 184.00 SPILLED 188.00 181.00 208.00 SPILLED 203.00 SPILLED 180.00 SPILLED SPILLED SPILLED 220.00 216.00 177.00 201.00 171.00 183.00 174.00 177.00 183.00 182.00 187.00 178.00 207.00 203.00 205.00 SPILLED 187.00 SPILLED 175.00 185.00 215.00 224.00 173.00 197.00 174.00 183.00 176.00 173.00 176.00 174.00 185.00 182.00 208.00 208.00 204.00 184.00 182.00 SPILLED 156.00 182.00 NOT TAKEN 216.00 179.00 195.00 187.00 187.00 176.00 181.00 181.00 171.00 186.00 167.00 203.00 213.00 211.00 181.00 189.00 SPILLED 175.00 185.00 207.00 185.00 198.00 192.00 187.00 181.00 186.00 187.00 174.00 184.00 188.00 206.00 213.00 210.00 194.00 190.00 SPILLED 182.00 189.00 212.00 186.00 206.00 178.00 199.00 175.00 182.00 170.00 181.00 185.00 183.00 203.00 212.00 220.00 192.00 185.00 SPILLED 179.00 182.00 217.00 174.00 197.00 202.00 179.00 185.00 185.00 173.00 184.00 185.00 204.00 189.00 220.00 214.00 198.00 182.00 238.00 181.00 183.00 212.00 183.00 182.00 191.00 172.00 182.00 174.00 183.00 188.00 171.00 186.00 188.00 196.00 213.00 190.00 177.00 229.00 168.00 177.00 209.00 185.00 189.00 198.00 174.00 178.00 179.00 171.00 173.00 168.00 SPILLED 188.00 197.00 199.00 189.00 181.00 214.00 179.00 179.00 208.00 176.00 184.00 181.00 183.00 175.00 180.00 172.00 174.00 179.00 SPILLED 194.00 208.00 229.00 191.00 176.00 209.00 170.00 177.00 GROUP: MALE 5 - 20 PPM C92751 C92753 C92754 C92755 C92756 C92757 C92758 C92760 177.00 154.00 153.00 170.00 224.00 169.00 161.00 184.00 164.00 SPILLED 166.00 170.00 244.00 165.00 162.00 199.00 185.00 202.00 158.00 171.00 237.00 161.00 164.00 187.00 184.00 SPILLED 142.00 175.00 SPILLED 163.00 162.00 188.00 196.00 SPILLED 159.00 176.00 SPILLED 165.00 166.00 181.00 199.00 218.00 154.00 179.00 239.00 176.00 167.00 179.00 196.00 SPILLED 162.00 180.00 SPILLED 182.00 175.00 189.00 190.00 199.00 149.00 173.00 245.00 188.00 157.00 176.00 187.00 159.00 152.00 168.00 SPILLED 173.00 154.00 185.00 188.00 177.00 157.00 172.00 215.00 175.00 155.00 176.00 1378 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 34 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 171.00 198.00 196.00 220.00 188.00 170.00 225.00 190.00 193.00 191.00 171.00 171.00 158.00 180.00 202.00 182.00 171.00 183.00 SPILLED 166.00 174.00 186.00 183.00 183.00 177.00 SPILLED 192.00 SPILLED 180.00 169.00 179.00 167.00 195.00 208.00 234.00 181.00 174.00 227.00 188.00 199.00 195.00 SPILLED 187.00 163.00 190.00 207.00 187.00 177.00 191.00 174.00 158.00 174.00 188.00 190.00 175.00 178.00 199.00 183.00 173.00 179.00 169.00 183.00 172.00 177.00 198.00 215.00 187.00 171.00 223.00 184.00 194.00 180.00 166.00 183.00 152.00 195.00 202.00 191.00 173.00 189.00 187.00 170.00 173.00 187.00 179.00 180.00 168.00 SPILLED 188.00 147.00 182.00 170.00 180.00 174.00 176.00 206.00 221.00 NOT TAKEN 175.00 188.00 184.00 197.00 180.00 181.00 181.00 153.00 172.00 205.00 190.00 NOT TAKEN 187.00 187.00 175.00 179.00 189.00 165.00 174.00 169.00 SPILLED 200.00 NOT TAKEN 185.00 173.00 177.00 164.00 177.00 206.00 212.00 183.00 224.00 191.00 201.00 183.00 178.00 187.00 159.00 203.00 199.00 190.00 192.00 181.00 173.00 182.00 187.00 187.00 178.00 181.00 SPILLED 198.00 179.00 176.00 183.00 177.00 175.00 204.00 225.00 176.00 SPILLED 186.00 199.00 182.00 167.00 188.00 161.00 192.00 204.00 177.00 190.00 178.00 161.00 179.00 195.00 189.00 180.00 186.00 198.00 189.00 166.00 182.00 190.00 186.00 191.00 208.00 211.00 189.00 239.00 196.00 220.00 184.00 188.00 187.00 160.00 190.00 212.00 189.00 185.00 185.00 162.00 172.00 192.00 176.00 175.00 188.00 199.00 194.00 175.00 189.00 185.00 179.00 171.00 206.00 213.00 188.00 238.00 179.00 200.00 173.00 184.00 181.00 153.00 186.00 198.00 173.00 172.00 182.00 168.00 172.00 190.00 172.00 162.00 178.00 196.00 178.00 168.00 178.00 180.00 175.00 178.00 217.00 217.00 177.00 235.00 184.00 188.00 189.00 184.00 189.00 167.00 161.00 199.00 178.00 173.00 194.00 160.00 166.00 199.00 183.00 167.00 183.00 215.00 188.00 171.00 185.00 169.00 188.00 181.00 211.00 213.00 175.00 242.00 SPILLED 199.00 187.00 176.00 185.00 163.00 175.00 201.00 178.00 180.00 188.00 167.00 165.00 193.00 185.00 164.00 182.00 218.00 180.00 171.00 183.00 178.00 1379 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 35 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92794 C92795 C92796 C92797 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 164.00 178.00 150.00 165.00 191.00 SPILLED 197.00 176.00 178.00 178.00 193.00 166.00 176.00 155.00 190.00 177.00 197.00 167.00 190.00 130.00 SPILLED 152.00 197.00 174.00 152.00 SPILLED 160.00 174.00 148.00 171.00 190.00 173.00 195.00 174.00 176.00 175.00 191.00 178.00 172.00 147.00 183.00 176.00 TECH SPILL 169.00 174.00 129.00 SPILLED 148.00 195.00 167.00 144.00 232.00 165.00 164.00 147.00 167.00 202.00 174.00 192.00 176.00 178.00 182.00 194.00 181.00 170.00 153.00 198.00 179.00 187.00 169.00 183.00 125.00 SPILLED 150.00 199.00 169.00 150.00 227.00 161.00 173.00 151.00 174.00 NOT TAKEN 171.00 191.00 175.00 179.00 188.00 190.00 167.00 173.00 154.00 196.00 181.00 197.00 NOT TAKEN 191.00 135.00 195.00 154.00 199.00 172.00 162.00 176.00 161.00 181.00 159.00 175.00 171.00 205.00 181.00 182.00 182.00 192.00 163.00 189.00 159.00 201.00 188.00 205.00 185.00 135.00 SPILLED 152.00 201.00 176.00 157.00 214.00 159.00 187.00 161.00 180.00 171.00 196.00 185.00 185.00 185.00 185.00 176.00 184.00 160.00 195.00 176.00 210.00 184.00 145.00 200.00 149.00 201.00 180.00 162.00 199.00 155.00 182.00 151.00 197.00 172.00 188.00 180.00 185.00 187.00 192.00 171.00 173.00 157.00 195.00 171.00 209.00 182.00 132.00 SPILLED 148.00 213.00 180.00 167.00 177.00 161.00 171.00 158.00 187.00 158.00 203.00 175.00 182.00 179.00 181.00 168.00 166.00 150.00 168.00 172.00 200.00 180.00 125.00 185.00 145.00 202.00 166.00 151.00 155.00 149.00 175.00 157.00 183.00 165.00 208.00 185.00 180.00 175.00 181.00 187.00 167.00 158.00 177.00 173.00 202.00 184.00 140.00 199.00 157.00 211.00 165.00 158.00 194.00 159.00 175.00 159.00 188.00 159.00 204.00 193.00 193.00 186.00 178.00 178.00 171.00 154.00 170.00 176.00 198.00 199.00 136.00 195.00 146.00 222.00 177.00 165.00 200.00 GROUP: MALE 6 - 20 PPM C92821 C92822 172.00 162.00 186.00 163.00 182.00 160.00 173.00 174.00 174.00 173.00 162.00 166.00 170.00 183.00 SPILLED 163.00 203.00 176.00 178.00 169.00 1380 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 36 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 175.00 182.00 243.00 174.00 177.00 180.00 196.00 182.00 189.00 SPILLED 179.00 176.00 160.00 198.00 182.00 174.00 232.00 153.00 167.00 189.00 195.00 143.00 194.00 194.00 213.00 170.00 SPILLED 181.00 168.00 191.00 164.00 174.00 182.00 211.00 202.00 178.00 176.00 201.00 186.00 195.00 161.00 177.00 174.00 157.00 179.00 178.00 174.00 236.00 152.00 156.00 180.00 185.00 132.00 186.00 194.00 213.00 169.00 SPILLED 178.00 170.00 180.00 159.00 170.00 182.00 215.00 188.00 188.00 181.00 203.00 192.00 192.00 163.00 181.00 165.00 156.00 175.00 177.00 181.00 242.00 160.00 120.00 184.00 183.00 136.00 SPILLED 194.00 212.00 180.00 SPILLED SPILLED 169.00 173.00 159.00 174.00 191.00 223.00 195.00 205.00 191.00 208.00 194.00 164.00 170.00 191.00 175.00 162.00 189.00 175.00 189.00 236.00 158.00 169.00 183.00 175.00 140.00 196.00 180.00 210.00 SPILLED SPILLED 185.00 176.00 165.00 165.00 181.00 187.00 224.00 189.00 199.00 187.00 210.00 194.00 197.00 164.00 188.00 177.00 171.00 190.00 184.00 180.00 248.00 162.00 171.00 166.00 186.00 144.00 188.00 186.00 216.00 172.00 214.00 181.00 188.00 170.00 160.00 187.00 193.00 SPILLED 188.00 195.00 180.00 206.00 187.00 200.00 153.00 186.00 178.00 169.00 177.00 171.00 177.00 248.00 161.00 161.00 184.00 200.00 151.00 190.00 196.00 206.00 166.00 221.00 SPILLED 188.00 180.00 170.00 154.00 189.00 243.00 205.00 190.00 194.00 208.00 144.00 192.00 162.00 185.00 185.00 172.00 180.00 195.00 178.00 237.00 153.00 160.00 187.00 209.00 158.00 195.00 192.00 211.00 172.00 SPILLED SPILLED 178.00 169.00 181.00 126.00 180.00 227.00 193.00 187.00 185.00 195.00 170.00 190.00 156.00 181.00 183.00 163.00 167.00 180.00 174.00 238.00 155.00 147.00 181.00 175.00 147.00 182.00 176.00 203.00 177.00 225.00 180.00 173.00 158.00 169.00 174.00 SPILLED 230.00 192.00 180.00 182.00 199.00 174.00 176.00 151.00 177.00 178.00 166.00 176.00 184.00 180.00 231.00 159.00 152.00 173.00 188.00 151.00 182.00 SPILLED 197.00 169.00 SPILLED SPILLED 184.00 165.00 172.00 164.00 SPILLED SPILLED 187.00 180.00 181.00 202.00 177.00 170.00 159.00 182.00 181.00 166.00 178.00 197.00 181.00 240.00 160.00 146.00 182.00 179.00 141.00 183.00 207.00 200.00 164.00 SPILLED SPILLED 210.00 186.00 178.00 1381 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 37 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92854 C92855 C92856 C92857 C92858 C92859 C92860 186.00 183.00 167.00 172.00 191.00 180.00 173.00 185.00 171.00 160.00 168.00 186.00 179.00 167.00 181.00 179.00 169.00 171.00 189.00 176.00 166.00 184.00 247.00 171.00 178.00 188.00 182.00 175.00 187.00 195.00 174.00 179.00 192.00 184.00 172.00 186.00 183.00 171.00 183.00 186.00 177.00 171.00 195.00 153.00 166.00 181.00 193.00 190.00 179.00 189.00 169.00 164.00 163.00 193.00 192.00 174.00 197.00 179.00 166.00 167.00 188.00 185.00 168.00 183.00 167.00 168.00 164.00 SPILLED 187.00 163.00 GROUP: FEMALE 1 - 0 PPM C92862 C92863 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92876 C92877 C92878 C92879 C92880 C92881 C92882 C92883 119.00 119.00 128.00 118.00 158.00 154.00 SPILLED 128.00 138.00 109.00 133.00 126.00 132.00 112.00 SPILLED SPILLED 148.00 133.00 151.00 103.00 154.00 122.00 130.00 154.00 126.00 150.00 149.00 SPILLED 132.00 SPILLED 102.00 133.00 126.00 142.00 110.00 SPILLED SPILLED 135.00 148.00 169.00 107.00 164.00 125.00 122.00 137.00 121.00 128.00 151.00 SPILLED 129.00 143.00 102.00 125.00 118.00 125.00 114.00 142.00 SPILLED 134.00 147.00 SPILLED 108.00 139.00 128.00 99.00 132.00 116.00 114.00 139.00 SPILLED 130.00 125.00 105.00 135.00 122.00 140.00 115.00 SPILLED SPILLED 162.00 NOT TAKEN 140.00 106.00 156.00 129.00 115.00 151.00 122.00 130.00 143.00 SPILLED 142.00 SPILLED 102.00 134.00 122.00 140.00 125.00 144.00 SPILLED 140.00 140.00 110.00 140.00 121.00 117.00 139.00 121.00 132.00 118.00 SPILLED 131.00 144.00 107.00 123.00 128.00 146.00 105.00 157.00 SPILLED 135.00 130.00 112.00 134.00 105.00 123.00 165.00 125.00 132.00 154.00 SPILLED 137.00 SPILLED 155.00 145.00 126.00 SPILLED 130.00 143.00 SPILLED 141.00 107.00 127.00 151.00 118.00 117.00 151.00 121.00 139.00 134.00 SPILLED 137.00 SPILLED SPILLED 114.00 115.00 136.00 125.00 147.00 SPILLED 140.00 156.00 94.00 139.00 119.00 110.00 142.00 127.00 151.00 118.00 SPILLED 138.00 141.00 SPILLED 122.00 117.00 148.00 134.00 123.00 SPILLED 135.00 143.00 116.00 125.00 124.00 145.00 151.00 128.00 167.00 127.00 SPILLED 146.00 129.00 154.00 114.00 133.00 142.00 114.00 126.00 SPILLED 145.00 139.00 115.00 130.00 1382 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 38 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92884 C92885 C92886 C92887 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92898 C92899 C92900 C92901 C92902 C92903 C92904 C92905 C92906 C92907 C92908 C92909 C92910 C92911 C92912 C92913 C92915 SPILLED 144.00 119.00 132.00 156.00 121.00 140.00 SPILLED 145.00 107.00 131.00 154.00 SPILLED 125.00 137.00 178.00 143.00 124.00 164.00 169.00 121.00 130.00 111.00 123.00 123.00 130.00 SPILLED 114.00 138.00 129.00 153.00 143.00 141.00 128.00 133.00 159.00 113.00 SPILLED SPILLED 153.00 121.00 135.00 133.00 SPILLED 135.00 142.00 177.00 108.00 115.00 162.00 168.00 SPILLED 129.00 SPILLED 118.00 131.00 126.00 SPILLED 117.00 135.00 121.00 148.00 128.00 143.00 122.00 138.00 157.00 104.00 142.00 SPILLED 146.00 119.00 135.00 145.00 143.00 127.00 134.00 152.00 117.00 111.00 172.00 165.00 119.00 127.00 107.00 115.00 134.00 130.00 SPILLED 113.00 125.00 116.00 SPILLED 129.00 135.00 120.00 NOT TAKEN 147.00 103.00 136.00 137.00 130.00 108.00 134.00 133.00 132.00 125.00 NOT TAKEN 147.00 SPILLED 105.00 183.00 NOT TAKEN 117.00 NOT TAKEN 100.00 122.00 116.00 125.00 SPILLED 109.00 132.00 117.00 151.00 135.00 145.00 120.00 149.00 112.00 136.00 141.00 131.00 107.00 125.00 142.00 130.00 123.00 168.00 SPILLED 115.00 163.00 116.00 116.00 111.00 119.00 128.00 SPILLED 117.00 144.00 124.00 147.00 138.00 137.00 127.00 158.00 113.00 152.00 131.00 135.00 111.00 116.00 125.00 132.00 124.00 171.00 120.00 119.00 149.00 106.00 SPILLED 119.00 126.00 121.00 SPILLED 117.00 SPILLED 115.00 132.00 159.00 141.00 136.00 165.00 129.00 SPILLED SPILLED 150.00 106.00 137.00 SPILLED 143.00 131.00 SPILLED 143.00 118.00 165.00 126.00 133.00 117.00 119.00 124.00 SPILLED 123.00 144.00 116.00 133.00 174.00 127.00 121.00 153.00 116.00 130.00 124.00 160.00 111.00 137.00 123.00 146.00 127.00 175.00 130.00 115.00 158.00 114.00 SPILLED 121.00 133.00 112.00 SPILLED 109.00 157.00 112.00 135.00 148.00 131.00 137.00 142.00 112.00 130.00 126.00 137.00 117.00 128.00 122.00 133.00 139.00 169.00 116.00 114.00 149.00 124.00 110.00 114.00 126.00 115.00 SPILLED 123.00 122.00 114.00 156.00 132.00 127.00 133.00 143.00 106.00 125.00 133.00 149.00 117.00 123.00 118.00 109.00 144.00 171.00 128.00 118.00 173.00 138.00 130.00 115.00 112.00 114.00 120.00 121.00 163.00 114.00 155.00 1383 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 39 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92916 C92917 C92918 C92920 C92921 C92922 C92923 C92924 C92925 C92927 C92928 C92929 C92930 169.00 174.00 123.00 205.00 SPILLED SPILLED 145.00 153.00 134.00 172.00 141.00 117.00 193.00 175.00 161.00 123.00 SPILLED 156.00 149.00 SPILLED SPILLED 137.00 111.00 142.00 119.00 195.00 SPILLED 165.00 123.00 144.00 146.00 135.00 127.00 SPILLED 139.00 SPILLED 134.00 121.00 197.00 183.00 162.00 132.00 SPILLED 151.00 136.00 122.00 SPILLED 129.00 132.00 138.00 115.00 195.00 SPILLED 166.00 125.00 144.00 SPILLED 132.00 124.00 151.00 129.00 155.00 144.00 118.00 190.00 192.00 158.00 121.00 177.00 131.00 126.00 123.00 SPILLED 128.00 157.00 148.00 120.00 186.00 172.00 170.00 116.00 173.00 145.00 148.00 138.00 SPILLED 141.00 151.00 157.00 117.00 191.00 148.00 162.00 111.00 148.00 153.00 146.00 123.00 137.00 121.00 SPILLED 136.00 126.00 183.00 159.00 156.00 132.00 146.00 148.00 145.00 119.00 118.00 146.00 157.00 126.00 120.00 176.00 170.00 160.00 119.00 125.00 159.00 142.00 132.00 138.00 140.00 159.00 148.00 145.00 187.00 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92942 C92943 C92944 C92946 C92947 141.00 SPILLED 165.00 150.00 127.00 108.00 133.00 138.00 118.00 SPILLED 145.00 131.00 178.00 109.00 144.00 130.00 SPILLED 162.00 SPILLED 124.00 120.00 145.00 SPILLED SPILLED SPILLED SPILLED 137.00 SPILLED 110.00 130.00 139.00 SPILLED 148.00 134.00 123.00 113.00 138.00 196.00 SPILLED SPILLED 151.00 144.00 SPILLED 115.00 136.00 129.00 SPILLED 134.00 SPILLED 101.00 108.00 138.00 SPILLED SPILLED SPILLED SPILLED 162.00 NOT TAKEN 99.00 98.00 143.00 SPILLED 142.00 130.00 117.00 114.00 140.00 146.00 154.00 176.00 156.00 153.00 109.00 125.00 144.00 146.00 155.00 131.00 120.00 113.00 139.00 SPILLED SPILLED 138.00 SPILLED 145.00 114.00 128.00 150.00 SPILLED 155.00 126.00 127.00 101.00 150.00 SPILLED SPILLED 130.00 147.00 130.00 94.00 133.00 150.00 173.00 156.00 127.00 123.00 98.00 144.00 SPILLED SPILLED 123.00 140.00 112.00 115.00 106.00 162.00 175.00 159.00 123.00 111.00 111.00 144.00 125.00 130.00 120.00 142.00 127.00 100.00 114.00 164.00 246.00 160.00 118.00 144.00 107.00 141.00 SPILLED SPILLED 120.00 142.00 151.00 117.00 115.00 1384 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 40 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92948 C92949 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 143.00 144.00 104.00 127.00 120.00 133.00 139.00 142.00 131.00 111.00 152.00 131.00 168.00 116.00 113.00 123.00 127.00 112.00 127.00 133.00 138.00 107.00 141.00 137.00 116.00 142.00 137.00 146.00 152.00 132.00 143.00 130.00 165.00 SPILLED 117.00 115.00 136.00 131.00 121.00 125.00 108.00 143.00 155.00 159.00 116.00 126.00 SPILLED 132.00 116.00 SPILLED 134.00 148.00 110.00 146.00 133.00 110.00 142.00 136.00 171.00 155.00 115.00 136.00 144.00 175.00 SPILLED 117.00 119.00 SPILLED 132.00 124.00 SPILLED 112.00 159.00 153.00 162.00 117.00 115.00 121.00 122.00 120.00 125.00 138.00 148.00 106.00 144.00 SPILLED 113.00 138.00 124.00 154.00 150.00 127.00 137.00 115.00 168.00 114.00 134.00 113.00 138.00 127.00 103.00 SPILLED 100.00 157.00 144.00 160.00 NOT TAKEN 125.00 SPILLED 120.00 115.00 NOT TAKEN 131.00 147.00 113.00 133.00 147.00 127.00 135.00 125.00 144.00 NOT TAKEN 125.00 133.00 138.00 163.00 115.00 125.00 108.00 131.00 131.00 129.00 134.00 110.00 139.00 149.00 164.00 115.00 129.00 125.00 111.00 133.00 152.00 101.00 145.00 146.00 123.00 135.00 130.00 138.00 122.00 135.00 133.00 175.00 120.00 124.00 117.00 132.00 132.00 133.00 131.00 119.00 151.00 144.00 158.00 116.00 207.00 125.00 121.00 133.00 153.00 107.00 158.00 145.00 121.00 144.00 137.00 SPILLED 117.00 130.00 146.00 166.00 126.00 132.00 122.00 125.00 136.00 137.00 131.00 124.00 156.00 149.00 149.00 119.00 SPILLED 125.00 124.00 132.00 135.00 105.00 154.00 137.00 116.00 148.00 135.00 133.00 105.00 137.00 149.00 155.00 SPILLED 131.00 109.00 116.00 147.00 126.00 SPILLED 113.00 154.00 SPILLED 151.00 107.00 SPILLED 132.00 109.00 131.00 135.00 96.00 140.00 167.00 107.00 130.00 143.00 133.00 123.00 142.00 133.00 137.00 SPILLED 128.00 107.00 123.00 148.00 132.00 129.00 108.00 222.00 182.00 149.00 117.00 140.00 CNTMD 105.00 138.00 134.00 113.00 138.00 138.00 100.00 137.00 134.00 126.00 118.00 112.00 131.00 96.00 109.00 121.00 121.00 126.00 141.00 135.00 122.00 118.00 141.00 149.00 150.00 127.00 164.00 141.00 96.00 114.00 131.00 102.00 141.00 157.00 99.00 127.00 134.00 134.00 109.00 155.00 1385 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 41 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92981 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 127.00 141.00 136.00 136.00 128.00 121.00 139.00 SPILLED 146.00 SPILLED 136.00 133.00 136.00 120.00 125.00 123.00 SPILLED 143.00 128.00 141.00 132.00 150.00 126.00 124.00 136.00 SPILLED 134.00 129.00 SPILLED 123.00 133.00 109.00 NOT TAKEN 145.00 SPILLED 146.00 131.00 126.00 126.00 130.00 127.00 140.00 153.00 139.00 120.00 126.00 125.00 SPILLED 130.00 133.00 SPILLED 139.00 123.00 162.00 137.00 144.00 123.00 130.00 161.00 124.00 154.00 125.00 128.00 134.00 116.00 127.00 163.00 126.00 111.00 129.00 127.00 125.00 117.00 153.00 207.00 138.00 144.00 135.00 118.00 165.00 111.00 124.00 SPILLED 195.00 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92997 C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93007 C93009 C93010 C93011 C93012 C93013 111.00 129.00 131.00 122.00 119.00 146.00 SPILLED 129.00 128.00 126.00 115.00 126.00 119.00 123.00 SPILLED 148.00 130.00 147.00 117.00 110.00 120.00 130.00 130.00 114.00 146.00 SPILLED 149.00 135.00 131.00 132.00 130.00 121.00 SPILLED 168.00 146.00 122.00 136.00 128.00 108.00 119.00 125.00 117.00 113.00 145.00 142.00 126.00 125.00 124.00 132.00 125.00 130.00 111.00 129.00 142.00 117.00 138.00 110.00 115.00 SPILLED NOT TAKEN 112.00 SPILLED 147.00 SPILLED NOT TAKEN 123.00 130.00 130.00 124.00 142.00 124.00 142.00 SPILLED 125.00 140.00 131.00 111.00 136.00 128.00 118.00 145.00 161.00 128.00 114.00 140.00 129.00 141.00 126.00 125.00 150.00 123.00 149.00 114.00 119.00 133.00 122.00 SPILLED 152.00 SPILLED 128.00 131.00 130.00 124.00 130.00 115.00 127.00 180.00 131.00 149.00 124.00 119.00 139.00 128.00 SPILLED 139.00 SPILLED 144.00 138.00 147.00 121.00 162.00 118.00 123.00 142.00 116.00 154.00 110.00 114.00 141.00 112.00 125.00 146.00 130.00 159.00 128.00 129.00 118.00 147.00 120.00 141.00 130.00 116.00 150.00 139.00 137.00 146.00 113.00 133.00 159.00 145.00 171.00 137.00 154.00 107.00 137.00 125.00 156.00 129.00 114.00 157.00 108.00 134.00 152.00 112.00 126.00 142.00 147.00 153.00 140.00 125.00 121.00 146.00 114.00 146.00 162.00 130.00 151.00 118.00 1386 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 42 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 C93029 C93030 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93046 135.00 134.00 99.00 144.00 136.00 140.00 131.00 135.00 134.00 SPILLED 139.00 166.00 139.00 122.00 133.00 SPILLED 136.00 123.00 SPILLED 149.00 145.00 SPILLED 129.00 SPILLED 152.00 SPILLED 165.00 137.00 142.00 125.00 116.00 155.00 SPILLED SPILLED 142.00 130.00 SPILLED 197.00 118.00 120.00 SPILLED 140.00 169.00 135.00 131.00 123.00 SPILLED SPILLED 131.00 SPILLED SPILLED 138.00 129.00 133.00 SPILLED 142.00 SPILLED SPILLED 138.00 149.00 115.00 169.00 139.00 SPILLED 118.00 146.00 128.00 SPILLED 137.00 124.00 122.00 SPILLED 131.00 167.00 123.00 126.00 SPILLED SPILLED 132.00 126.00 SPILLED 137.00 131.00 123.00 130.00 131.00 144.00 SPILLED 163.00 135.00 148.00 114.00 149.00 133.00 SPILLED 118.00 155.00 NOT TAKEN SPILLED 154.00 128.00 131.00 NOT TAKEN 130.00 166.00 109.00 157.00 SPILLED SPILLED 121.00 115.00 SPILLED 130.00 NOT TAKEN SPILLED 134.00 133.00 140.00 131.00 SPILLED 149.00 143.00 120.00 138.00 144.00 140.00 SPILLED 147.00 SPILLED 143.00 116.00 118.00 146.00 182.00 120.00 123.00 SPILLED SPILLED 144.00 124.00 SPILLED 126.00 136.00 134.00 134.00 132.00 SPILLED 146.00 129.00 157.00 120.00 142.00 131.00 SPILLED 146.00 145.00 147.00 136.00 128.00 125.00 135.00 134.00 121.00 125.00 134.00 SPILLED SPILLED 133.00 SPILLED 135.00 117.00 166.00 140.00 125.00 SPILLED 154.00 112.00 147.00 SPILLED 167.00 140.00 SPILLED SPILLED 140.00 158.00 145.00 149.00 119.00 127.00 174.00 130.00 120.00 SPILLED SPILLED 135.00 139.00 SPILLED 114.00 134.00 116.00 128.00 127.00 119.00 173.00 170.00 139.00 125.00 149.00 116.00 SPILLED SPILLED 128.00 SPILLED 161.00 138.00 118.00 129.00 136.00 130.00 124.00 130.00 SPILLED 136.00 135.00 194.00 129.00 144.00 107.00 128.00 126.00 144.00 180.00 158.00 156.00 SPILLED 148.00 141.00 132.00 193.00 146.00 155.00 144.00 134.00 117.00 132.00 168.00 129.00 119.00 SPILLED 171.00 124.00 137.00 SPILLED 131.00 139.00 119.00 123.00 131.00 155.00 170.00 166.00 140.00 195.00 TECH SPILL 149.00 152.00 167.00 145.00 155.00 119.00 134.00 138.00 126.00 140.00 134.00 119.00 SPILLED SPILLED 146.00 138.00 162.00 134.00 123.00 114.00 129.00 132.00 141.00 160.00 155.00 155.00 SPILLED SPILLED 1387 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 43 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93047 C93048 C93049 C93050 155.00 128.00 124.00 141.00 152.00 SPILLED 121.00 SPILLED 142.00 133.00 121.00 153.00 149.00 126.00 123.00 SPILLED 157.00 125.00 121.00 150.00 152.00 143.00 124.00 164.00 138.00 130.00 123.00 148.00 130.00 129.00 115.00 150.00 141.00 SPILLED 120.00 140.00 176.00 127.00 122.00 145.00 GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 C93057 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93068 C93069 C93071 C93072 C93073 C93074 C93076 C93077 C93078 128.00 SPILLED 144.00 SPILLED SPILLED 126.00 130.00 136.00 141.00 137.00 128.00 122.00 132.00 118.00 137.00 129.00 144.00 115.00 160.00 143.00 180.00 SPILLED 135.00 135.00 143.00 SPILLED 132.00 SPILLED 191.00 125.00 129.00 136.00 140.00 116.00 121.00 112.00 122.00 131.00 126.00 131.00 143.00 112.00 159.00 141.00 SPILLED 132.00 127.00 170.00 129.00 SPILLED 129.00 132.00 137.00 128.00 134.00 129.00 130.00 125.00 121.00 127.00 121.00 116.00 127.00 124.00 146.00 110.00 SPILLED 144.00 156.00 SPILLED 125.00 SPILLED NOT TAKEN SPILLED 143.00 142.00 SPILLED 125.00 134.00 133.00 134.00 123.00 120.00 119.00 NOT TAKEN 121.00 126.00 126.00 141.00 NOT TAKEN 139.00 137.00 SPILLED 108.00 119.00 SPILLED SPILLED 133.00 127.00 134.00 129.00 137.00 130.00 136.00 134.00 124.00 125.00 118.00 131.00 126.00 138.00 SPILLED 146.00 155.00 SPILLED 116.00 138.00 SPILLED 151.00 SPILLED 132.00 130.00 128.00 141.00 128.00 129.00 121.00 121.00 122.00 126.00 128.00 140.00 142.00 151.00 165.00 SPILLED 119.00 144.00 SPILLED 145.00 133.00 133.00 136.00 120.00 129.00 118.00 144.00 125.00 112.00 118.00 SPILLED 125.00 147.00 149.00 145.00 171.00 136.00 141.00 165.00 SPILLED 150.00 133.00 124.00 128.00 132.00 122.00 124.00 153.00 121.00 108.00 117.00 SPILLED 134.00 165.00 141.00 139.00 155.00 134.00 140.00 130.00 134.00 144.00 135.00 138.00 145.00 131.00 125.00 111.00 156.00 129.00 113.00 114.00 158.00 116.00 146.00 147.00 137.00 148.00 135.00 139.00 134.00 SPILLED 145.00 134.00 161.00 146.00 136.00 134.00 125.00 156.00 SPILLED 121.00 104.00 SPILLED 134.00 155.00 151.00 133.00 140.00 118.00 136.00 141.00 1388 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 44 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93079 C93080 C93081 C93082 C93083 C93085 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 144.00 128.00 116.00 121.00 135.00 132.00 127.90 122.00 108.00 147.00 123.00 126.00 132.00 133.00 132.00 123.00 149.00 SPILLED 120.00 123.00 156.00 119.00 SPILLED 137.00 SPILLED 137.00 122.00 125.00 120.00 133.00 199.00 151.00 129.00 120.00 121.00 134.00 133.00 155.00 126.00 132.00 135.00 113.00 130.00 130.00 134.00 135.00 SPILLED 159.00 SPILLED 131.00 114.00 149.00 111.00 SPILLED 130.00 211.00 143.00 113.00 117.00 SPILLED 129.00 200.00 151.00 125.00 125.00 123.00 138.00 SPILLED 129.00 107.00 125.00 127.00 117.00 123.00 115.00 160.00 123.00 135.00 154.00 SPILLED 117.00 123.00 156.00 98.00 147.00 139.00 192.00 134.00 110.00 110.00 114.00 115.00 212.00 145.00 119.00 115.00 121.00 137.00 NOT TAKEN SPILLED 111.00 132.00 115.00 105.00 130.00 120.00 113.00 122.00 126.00 SPILLED SPILLED 134.00 118.00 143.00 107.00 SPILLED 130.00 SPILLED 123.00 108.00 115.00 109.00 NOT TAKEN SPILLED 167.00 131.00 122.00 124.00 145.00 136.00 112.00 129.00 138.00 127.00 128.00 124.00 153.00 132.00 128.00 154.00 SPILLED 117.00 118.00 145.00 113.00 154.00 124.00 SPILLED 129.00 119.00 118.00 116.00 202.00 145.00 120.00 112.00 133.00 141.00 135.00 119.00 137.00 131.00 116.00 120.00 123.00 161.00 143.00 128.00 150.00 SPILLED 122.00 125.00 154.00 108.00 155.00 127.00 184.00 130.00 115.00 118.00 119.00 SPILLED 149.00 160.00 121.00 136.00 131.00 138.00 119.00 136.00 141.00 114.00 136.00 147.00 SPILLED 127.00 123.00 162.00 SPILLED 125.00 109.00 157.00 SPILLED 168.00 144.00 180.00 130.00 121.00 120.00 SPILLED 194.00 129.00 133.00 117.00 153.00 133.00 123.00 109.00 128.00 129.00 107.00 136.00 134.00 SPILLED 111.00 110.00 166.00 141.00 120.00 129.00 142.00 103.00 151.00 100.00 178.00 118.00 117.00 108.00 110.00 199.00 142.00 SPILLED 130.00 139.00 138.00 126.00 108.00 139.00 124.00 108.00 144.00 138.00 117.00 119.00 111.00 153.00 138.00 111.00 134.00 131.00 109.00 SPILLED 134.00 165.00 114.00 125.00 114.00 103.00 210.00 131.00 SPILLED 118.00 145.00 127.00 120.00 122.00 139.00 129.00 104.00 119.00 148.00 144.00 126.00 109.00 136.00 120.00 142.00 137.00 109.00 159.00 182.00 163.00 117.00 120.00 118.00 105.00 169.00 1389 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 45 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93115 C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93143 C93145 132.00 124.00 124.00 113.00 SPILLED 120.00 116.00 111.00 107.00 101.00 130.00 128.00 117.00 108.00 121.00 120.00 133.00 136.00 130.00 124.00 115.00 123.00 128.00 122.00 121.00 109.00 124.00 147.00 146.00 SPILLED 135.00 126.00 118.00 119.00 116.00 121.00 126.00 112.00 110.00 125.00 107.00 134.00 130.00 122.00 99.00 126.00 129.00 126.00 145.00 118.00 117.00 118.00 125.00 131.00 126.00 126.00 122.00 120.00 142.00 139.00 180.00 119.00 115.00 109.00 107.00 111.00 111.00 108.00 116.00 105.00 117.00 99.00 102.00 141.00 120.00 96.00 112.00 127.00 122.00 131.00 121.00 111.00 106.00 114.00 131.00 119.00 123.00 116.00 121.00 129.00 145.00 184.00 SPILLED NOT TAKEN 119.00 110.00 113.00 110.00 117.00 123.00 103.00 102.00 97.00 127.00 106.00 123.00 105.00 NOT TAKEN 123.00 131.00 132.00 121.00 118.00 122.00 120.00 115.00 127.00 130.00 114.00 123.00 136.00 132.00 NOT TAKEN 131.00 114.00 129.00 109.00 117.00 SPILLED 125.00 110.00 124.00 100.00 128.00 104.00 118.00 109.00 121.00 119.00 140.00 119.00 123.00 121.00 120.00 142.00 131.00 130.00 116.00 119.00 150.00 139.00 143.00 119.00 124.00 110.00 116.00 120.00 135.00 103.00 110.00 100.00 129.00 118.00 117.00 109.00 123.00 136.00 139.00 SPILLED 124.00 128.00 120.00 130.00 135.00 125.00 121.00 117.00 155.00 156.00 145.00 SPILLED 119.00 112.00 118.00 SPILLED 134.00 111.00 121.00 108.00 129.00 117.00 124.00 117.00 122.00 163.00 137.00 126.00 143.00 SPILLED 132.00 121.00 136.00 135.00 126.00 SPILLED 155.00 143.00 123.00 120.00 114.00 112.00 122.00 123.00 128.00 105.00 120.00 104.00 123.00 122.00 105.00 113.00 116.00 160.00 133.00 129.00 129.00 SPILLED 117.00 102.00 144.00 123.00 121.00 114.00 147.00 132.00 SPILLED 127.00 111.00 105.00 115.00 131.00 93.00 108.00 119.00 99.00 138.00 105.00 109.00 121.00 107.00 118.00 114.00 128.00 128.00 SPILLED 121.00 116.00 139.00 121.00 119.00 117.00 139.00 144.00 163.00 130.00 123.00 101.00 124.00 145.00 127.00 110.00 116.00 98.00 159.00 101.00 108.00 123.00 130.00 133.00 145.00 130.00 127.00 121.00 113.00 144.00 146.00 120.00 110.00 119.00 152.00 129.00 SPILLED 1390 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 46 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93146 C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 112.00 110.00 143.00 124.00 SPILLED 122.00 126.00 SPILLED 122.00 SPILLED 113.00 134.00 124.00 141.00 128.00 SPILLED 109.00 114.00 109.00 130.00 125.00 108.00 122.00 SPILLED SPILLED 120.00 118.00 SPILLED 113.00 113.00 SPILLED 109.00 121.00 135.00 SPILLED 161.00 120.00 130.00 217.00 117.00 SPILLED 108.00 139.00 124.00 153.00 124.00 SPILLED 112.00 116.00 116.00 138.00 125.00 SPILLED 120.00 172.00 SPILLED 123.00 117.00 SPILLED 123.00 115.00 SPILLED 112.00 116.00 143.00 SPILLED 150.00 116.00 125.00 SPILLED 120.00 210.00 108.00 123.00 128.00 147.00 SPILLED 123.00 111.00 112.00 110.00 128.00 126.00 114.00 116.00 126.00 117.00 132.00 SPILLED SPILLED 109.00 115.00 113.00 SPILLED 116.00 131.00 148.00 129.00 117.00 129.00 SPILLED 114.00 NOT TAKEN 105.00 120.00 126.00 148.00 117.00 100.00 110.00 106.00 117.00 120.00 128.00 121.00 NOT TAKEN SPILLED SPILLED SPILLED 110.00 SPILLED 115.00 117.00 109.00 102.00 119.00 137.00 122.00 151.00 121.00 131.00 SPILLED 120.00 107.00 125.00 122.00 150.00 136.00 118.00 110.00 115.00 115.00 139.00 127.00 117.00 168.00 136.00 123.00 115.00 SPILLED 112.00 112.00 115.00 111.00 120.00 137.00 119.00 133.00 117.00 123.00 SPILLED 119.00 107.00 124.00 128.00 146.00 123.00 SPILLED 109.00 121.00 113.00 124.00 129.00 124.00 151.00 120.00 122.00 117.00 SPILLED 112.00 118.00 112.00 125.00 121.00 144.00 124.00 144.00 128.00 SPILLED SPILLED 115.00 108.00 132.00 136.00 152.00 126.00 SPILLED 143.00 113.00 115.00 136.00 124.00 SPILLED SPILLED 116.00 126.00 113.00 SPILLED 112.00 128.00 SPILLED 101.00 116.00 153.00 113.00 139.00 125.00 SPILLED 124.00 109.00 106.00 118.00 133.00 137.00 119.00 SPILLED 131.00 114.00 110.00 146.00 135.00 102.00 SPILLED 111.00 136.00 137.00 SPILLED 113.00 126.00 146.00 85.00 111.00 143.00 110.00 142.00 117.00 SPILLED 136.00 108.00 108.00 130.00 134.00 170.00 126.00 129.00 109.00 111.00 110.00 SPILLED 129.00 111.00 220.00 123.00 134.00 115.00 SPILLED 105.00 124.00 112.00 103.00 111.00 135.00 120.00 137.00 116.00 131.00 114.00 117.00 116.00 122.00 134.00 178.00 125.00 136.00 109.00 114.00 116.00 121.00 122.00 115.00 190.00 132.00 122.00 105.00 SPILLED 109.00 120.00 110.00 1391 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 47 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93178 C93179 C93180 115.00 126.00 126.00 115.00 127.00 133.00 119.00 135.00 134.00 103.00 111.00 137.00 126.00 116.00 148.00 118.00 133.00 138.00 125.00 122.00 151.00 110.00 108.00 137.00 108.00 113.00 131.00 105.00 117.00 132.00 GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 127.00 105.00 126.00 114.00 123.00 107.00 135.00 122.00 121.00 129.00 128.00 121.00 123.00 158.00 121.00 119.00 103.00 115.00 117.00 SPILLED 111.00 108.00 125.00 128.00 168.00 126.00 108.00 128.00 113.00 140.00 105.00 127.00 123.00 118.00 132.00 127.00 124.00 125.00 165.00 120.00 110.00 115.00 133.00 119.00 SPILLED 134.00 114.00 126.00 123.00 133.00 132.00 119.00 131.00 112.00 SPILLED 105.00 128.00 131.00 115.00 124.00 128.00 116.00 126.00 165.00 121.00 116.00 108.00 133.00 118.00 SPILLED 125.00 SPILLED 115.00 124.00 129.00 124.00 112.00 127.00 113.00 137.00 119.00 126.00 124.00 134.00 127.00 131.00 114.00 134.00 SPILLED 120.00 125.00 111.00 125.00 109.00 SPILLED 117.00 SPILLED 136.00 127.00 133.00 126.00 116.00 135.00 115.00 130.00 110.00 136.00 125.00 113.00 130.00 127.00 120.00 123.00 158.00 121.00 118.00 108.00 124.00 111.00 180.00 123.00 106.00 146.00 122.00 138.00 122.00 105.00 126.00 116.00 143.00 112.00 SPILLED 138.00 121.00 123.00 133.00 120.00 131.00 178.00 116.00 120.00 100.00 124.00 112.00 SPILLED SPILLED 144.00 133.00 124.00 124.00 135.00 123.00 130.00 120.00 138.00 124.00 170.00 142.00 123.00 128.00 139.00 SPILLED 144.00 SPILLED SPILLED 120.00 SPILLED 130.00 117.00 145.00 SPILLED 114.00 166.00 126.00 132.00 127.00 104.00 125.00 121.00 143.00 107.00 152.00 138.00 115.00 127.00 140.00 112.00 146.00 120.00 117.00 125.00 SPILLED 117.00 108.00 130.00 SPILLED 104.00 137.00 118.00 117.00 124.00 102.00 129.00 115.00 66.00 96.00 144.00 138.00 108.00 130.00 128.00 119.00 135.00 125.00 125.00 111.00 93.00 116.00 109.00 116.00 169.00 99.00 143.00 118.00 111.00 125.00 106.00 117.00 112.00 148.00 120.00 135.00 131.00 115.00 142.00 141.00 127.00 143.00 134.00 125.00 118.00 99.00 129.00 117.00 117.00 160.00 103.00 136.00 119.00 116.00 1392 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 48 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 127.00 120.00 122.00 SPILLED 122.00 108.00 156.00 129.00 121.00 120.00 113.00 144.00 137.00 137.00 154.00 SPILLED 133.00 116.00 SPILLED 126.00 113.00 137.00 113.00 118.00 122.00 111.00 SPILLED 135.00 107.00 SPILLED 113.00 125.00 104.00 SPILLED 140.00 108.00 120.00 125.00 116.00 117.00 111.00 SPILLED 137.00 151.00 190.00 SPILLED 113.00 110.00 SPILLED 126.00 113.00 125.00 119.00 121.00 118.00 106.00 SPILLED SPILLED 129.00 SPILLED 125.00 114.00 111.00 117.00 120.00 105.00 134.00 108.00 118.00 117.00 113.00 SPILLED 135.00 123.00 187.00 SPILLED 114.00 109.00 SPILLED 134.00 113.00 134.00 107.00 123.00 114.00 105.00 146.00 128.00 125.00 SPILLED SPILLED 124.00 109.00 SPILLED SPILLED 112.00 140.00 SPILLED 127.00 129.00 120.00 SPILLED SPILLED 137.00 SPILLED SPILLED 112.00 101.00 SPILLED 132.00 97.00 122.00 SPILLED 125.00 117.00 123.00 SPILLED SPILLED 124.00 SPILLED 129.00 115.00 106.00 169.00 134.00 98.00 123.00 107.00 122.00 114.00 126.00 132.00 130.00 122.00 134.00 131.00 123.00 103.00 184.00 141.00 98.00 133.00 110.00 119.00 110.00 132.00 137.00 110.00 144.00 155.00 1393 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 49 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: C92501 C92502 C92503 C92504 C92505 C92507 C92508 C92510 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92522 C92523 C92525 C92527 C92530 C92531 C92533 C92534 C92535 C92536 C92537 C92538 C92539 C92540 C92541 MALE 1 - 0 PPM 192.00 182.00 168.00 167.00 168.00 170.00 166.00 167.00 188.00 205.00 158.00 164.00 167.00 164.00 190.00 179.00 183.00 194.00 189.00 186.00 204.00 202.00 161.00 163.00 161.00 161.00 206.00 212.00 214.00 217.00 160.00 159.00 182.00 184.00 163.00 163.00 174.00 177.00 175.00 176.00 171.00 172.00 225.00 206.00 189.00 165.00 196.00 198.00 173.00 154.00 183.00 180.00 176.00 165.00 209.00 213.00 216.00 225.00 210.00 206.00 210.00 199.00 190.00 210.00 196.00 159.00 172.00 175.00 188.00 163.00 164.00 189.00 183.00 188.00 186.00 104.00 170.00 207.00 208.00 155.00 187.00 173.00 177.00 175.00 178.00 SPILLED 145.00 197.00 167.00 110.00 177.00 216.00 231.00 187.00 208.00 193.00 197.00 162.00 167.00 186.00 180.00 157.00 178.00 179.00 175.00 193.00 189.00 173.00 206.00 208.00 153.00 193.00 154.00 171.00 178.00 182.00 209.00 SPILLED 191.00 171.00 196.00 172.00 218.00 237.00 209.00 200.00 200.00 197.00 164.00 83.00 156.00 182.00 159.00 179.00 169.00 129.00 194.00 176.00 170.00 204.00 214.00 152.00 178.00 173.00 177.00 165.00 169.00 224.00 182.00 197.00 135.00 198.00 186.00 SPILLED 231.00 204.00 206.00 192.00 172.00 203.00 195.00 200.00 SPILLED 193.00 189.00 172.00 222.00 164.00 186.00 182.00 180.00 183.00 227.00 SPILLED 193.00 203.00 195.00 239.00 252.00 200.00 211.00 170.00 190.00 190.00 191.00 206.00 186.00 164.00 169.00 233.00 173.00 197.00 173.00 44.00 172.00 236.00 SPILLED 204.00 197.00 189.00 220.00 261.00 208.00 216.00 174.00 179.00 192.00 185.00 205.00 184.00 229.00 173.00 220.00 183.00 177.00 175.00 48.00 195.00 190.00 168.00 198.00 212.00 195.00 243.00 225.00 202.00 210.00 181.00 182.00 191.00 184.00 207.00 195.00 231.00 175.00 143.80 160.50 186.70 172.60 207.20 194.90 219.70 176.40 235.00 187.00 139.00 190.00 130.00 193.00 226.00 NOT TAKEN 201.00 204.00 183.00 241.00 243.00 210.00 217.00 228.10 189.10 SPILLED 168.00 NOT TAKEN 196.00 227.90 211.80 192.40 190.60 204.40 250.10 210.10 213.40 1394 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 50 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 C92563 C92564 C92565 C92567 C92568 C92569 C92570 185.00 165.00 180.00 183.00 179.00 184.00 154.00 225.00 166.00 185.00 140.00 154.00 194.00 176.00 189.00 187.00 178.00 190.00 180.00 181.00 202.00 196.00 190.00 175.00 186.00 162.00 179.00 187.00 168.00 182.00 182.00 181.00 183.00 161.00 219.00 168.00 196.00 SPILLED 154.00 205.00 165.00 181.00 194.00 183.00 200.00 181.00 175.00 237.00 195.00 187.00 178.00 185.00 168.00 183.00 193.00 163.00 184.00 213.00 184.00 183.00 162.00 224.00 161.00 192.00 124.00 154.00 205.00 184.00 192.00 208.00 180.00 201.00 176.00 178.00 240.00 195.00 195.00 179.00 186.00 175.00 175.00 200.00 172.00 164.00 199.00 180.00 178.00 166.00 216.00 155.00 204.00 130.00 157.00 188.00 183.00 171.00 189.00 175.00 198.00 175.00 183.00 233.00 194.00 188.00 166.00 184.00 160.00 173.00 185.00 166.00 177.00 191.00 173.00 177.00 157.00 215.00 159.00 199.00 102.00 150.00 202.00 168.00 180.00 SPILLED 178.00 210.00 178.00 142.00 SPILLED SPILLED 183.00 175.00 183.00 175.00 128.00 188.00 179.00 173.00 197.00 189.00 190.00 167.00 228.00 170.00 211.00 124.00 156.00 152.00 96.00 196.00 231.00 191.00 200.00 186.00 SPILLED 193.00 192.00 175.00 193.00 178.00 211.00 179.00 175.00 211.00 190.00 192.00 169.00 221.00 169.00 202.00 100.00 161.00 NOT TAKEN 56.00 195.00 198.00 190.00 208.00 141.00 SPILLED 198.00 206.00 193.00 189.00 172.00 205.00 182.00 181.00 206.00 199.00 181.00 172.00 212.00 177.00 173.00 149.00 193.00 196.00 185.00 205.00 252.00 192.00 193.00 186.00 191.00 181.00 200.00 180.00 190.00 208.00 209.00 169.00 170.00 221.00 190.00 216.00 171.00 200.00 197.00 190.00 222.00 240.00 139.00 181.00 192.00 200.00 175.00 199.70 175.90 177.60 220.60 199.10 167.30 169.90 224.80 193.90 202.90 167.40 197.40 169.30 191.80 221.90 261.00 152.40 197.80 211.00 174.90 1395 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 51 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92594 C92595 C92596 C92597 C92598 C92599 C92601 C92602 C92604 C92605 C92606 185.00 200.00 189.00 200.00 208.00 187.00 195.00 178.00 190.00 153.00 163.00 174.00 36.00 206.00 209.00 172.00 186.00 187.00 169.00 183.00 179.00 166.00 189.00 177.00 185.00 208.00 182.00 170.00 192.00 187.00 180.00 190.00 200.00 160.00 194.00 193.00 199.00 185.00 180.00 183.00 150.00 167.00 164.00 210.00 190.00 157.00 181.00 190.00 169.00 174.00 179.00 156.00 181.00 187.00 187.00 211.00 181.00 155.00 205.00 195.00 189.00 182.00 193.00 208.00 187.00 199.00 194.00 191.00 198.00 189.00 154.00 169.00 190.00 208.00 203.00 175.00 194.00 188.00 164.00 187.00 174.00 155.00 190.00 185.00 185.00 217.00 184.00 166.00 194.00 174.00 188.00 191.00 197.00 212.00 197.00 190.00 196.00 187.00 188.00 189.00 170.00 166.00 191.00 196.00 196.00 166.00 192.00 192.00 179.00 181.00 183.00 159.00 191.00 195.00 189.00 210.00 178.00 177.00 204.00 181.00 184.00 182.00 172.00 196.00 192.00 199.00 192.00 195.00 190.00 193.00 154.00 178.00 SPILLED 198.00 192.00 162.00 195.00 193.00 172.00 183.00 176.00 169.00 193.00 185.00 180.00 196.00 181.00 174.00 204.00 180.00 186.00 186.00 208.00 212.00 215.00 215.00 216.00 199.00 203.00 214.00 155.00 185.00 SPILLED 206.00 214.00 177.00 204.00 197.00 180.00 203.00 188.00 167.00 193.00 181.00 179.00 188.00 174.00 205.00 200.00 199.00 202.00 214.00 201.00 213.00 210.00 214.00 209.00 212.00 216.00 167.00 179.00 NOT TAKEN 188.00 211.00 182.00 204.00 199.00 172.00 197.00 198.00 170.00 190.00 176.00 185.00 178.00 193.00 214.00 191.00 196.00 193.00 SPILLED 196.00 211.00 204.00 210.00 195.00 206.00 217.00 161.00 189.00 204.00 199.00 186.00 200.00 194.00 182.00 195.00 180.00 173.00 173.00 167.00 177.00 186.00 194.00 211.00 202.00 190.00 203.00 214.00 115.00 216.00 207.00 219.00 188.00 228.00 231.00 SPILLED 180.00 50.00 210.00 181.00 211.00 207.00 189.00 203.00 206.00 155.00 41.00 180.00 174.00 200.00 202.00 199.00 189.00 195.00 173.20 207.20 193.10 215.70 203.20 223.70 164.80 144.50 241.10 161.80 193.60 0.50 197.20 169.20 200.90 196.30 182.00 203.00 189.00 174.00 186.00 185.00 188.00 197.00 154.00 205.00 210.00 1396 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 52 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92607 C92608 C92609 C92611 C92612 C92614 C92615 C92617 C92619 C92620 C92622 C92623 C92624 C92625 C92626 C92628 C92629 C92630 213.00 164.00 183.00 160.00 186.00 SPILLED 190.00 209.00 147.00 160.00 187.00 151.00 195.00 201.00 140.00 217.00 212.00 209.00 219.00 205.00 196.00 156.00 175.00 SPILLED 195.00 214.00 155.00 162.00 175.00 148.00 195.00 197.00 189.00 SPILLED 214.00 212.00 209.00 188.00 202.00 153.00 178.00 246.00 186.00 216.00 163.00 154.00 184.00 142.00 186.00 199.00 169.00 239.00 215.00 207.00 207.00 219.00 189.00 161.00 176.00 245.00 195.00 203.00 175.00 171.00 186.00 152.00 201.00 204.00 197.00 244.00 216.00 207.00 192.00 223.00 193.00 160.00 176.00 SPILLED 189.00 202.00 154.00 175.00 185.00 154.00 204.00 189.00 199.00 120.00 210.00 202.00 219.00 217.00 202.00 165.00 195.00 SPILLED 196.00 232.00 SPILLED 171.00 196.00 158.00 211.00 211.00 167.00 SPILLED 224.00 215.00 242.00 211.00 196.00 160.00 194.00 261.00 195.00 227.00 176.00 166.00 201.00 153.00 207.00 210.00 171.00 55.00 218.00 197.00 226.00 191.00 192.00 171.00 191.00 SPILLED 175.00 212.00 169.00 174.00 198.00 158.00 201.00 194.00 SPILLED 203.00 206.00 234.00 184.00 195.00 176.00 185.00 SPILLED 204.00 251.00 181.00 183.00 208.00 157.00 200.00 215.00 110.00 180.00 195.00 226.00 81.00 196.00 165.00 147.00 SPILLED 201.00 261.00 177.30 162.30 204.00 150.90 196.60 147.80 107.40 129.80 208.90 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92641 177.00 207.00 175.00 174.00 186.00 174.00 166.00 223.00 177.00 180.00 173.00 195.00 192.00 172.00 186.00 174.00 168.00 227.00 178.00 211.00 176.00 193.00 176.00 185.00 190.00 192.00 163.00 213.00 179.00 195.00 176.00 201.00 188.00 184.00 183.00 203.00 171.00 235.00 180.00 189.00 168.00 197.00 176.00 181.00 190.00 217.00 171.00 222.00 178.00 213.00 178.00 204.00 186.00 200.00 193.00 226.00 168.00 221.00 188.00 190.00 177.00 180.00 192.00 202.00 198.00 SPILLED 187.00 236.00 196.00 118.00 181.00 157.00 193.00 196.00 171.00 SPILLED 182.00 223.00 196.00 187.00 189.00 195.00 203.00 194.00 198.00 SPILLED 190.00 227.00 183.00 208.00 188.00 226.00 190.00 203.00 179.00 SPILLED 179.00 220.00 139.00 172.00 1397 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92642 C92643 C92644 C92647 C92648 C92649 C92651 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92663 C92664 C92665 C92666 C92667 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92677 C92679 C92680 161.00 167.00 166.00 161.00 161.00 189.00 187.00 195.00 184.00 199.00 177.00 187.00 190.00 195.00 195.00 151.00 203.00 186.00 191.00 222.00 196.00 179.00 160.00 187.00 164.00 164.00 170.00 183.00 184.00 206.00 176.00 158.00 166.00 164.00 168.00 156.00 179.00 189.00 198.00 180.00 218.00 179.00 176.00 189.00 203.00 SPILLED 171.00 201.00 188.00 195.00 214.00 204.00 186.00 161.00 185.00 182.00 168.00 182.00 187.00 186.00 207.00 186.00 160.00 168.00 159.00 172.00 165.00 190.00 193.00 199.00 169.00 205.00 178.00 177.00 193.00 214.00 203.00 170.00 203.00 188.00 201.00 196.00 195.00 177.00 159.00 181.00 169.00 178.00 180.00 183.00 178.00 221.00 178.00 167.00 184.00 168.00 171.00 152.00 SPILLED 203.00 187.00 167.00 209.00 172.00 175.00 194.00 207.00 215.00 171.00 207.00 192.00 189.00 193.00 195.00 191.00 157.00 185.00 172.00 162.00 171.00 184.00 188.00 239.00 185.00 172.00 182.00 168.00 166.00 154.00 185.00 216.00 207.00 172.00 210.00 176.00 181.00 188.00 212.00 SPILLED 178.00 210.00 186.00 189.00 193.00 184.00 180.00 154.00 178.00 178.00 166.00 192.00 183.00 182.00 206.00 182.00 172.00 SPILLED 180.00 177.00 170.00 216.00 220.00 182.00 186.00 SPILLED 191.00 194.00 207.00 226.00 SPILLED 184.00 218.00 197.00 200.00 199.00 202.00 190.00 165.00 205.00 186.00 191.00 193.00 198.00 197.00 204.00 176.00 200.00 185.00 176.00 169.00 196.00 230.00 227.00 195.00 209.00 189.00 185.00 218.00 232.00 SPILLED 188.00 TECH SPILL 197.00 213.00 205.00 203.00 194.00 176.00 193.00 196.00 199.00 198.00 191.00 211.00 196.00 161.00 SPILLED 180.00 183.00 172.00 176.00 198.00 157.00 213.00 203.00 196.00 191.00 205.00 227.00 206.00 203.00 SPILLED 194.00 SPILLED 194.00 202.00 200.00 198.00 178.00 202.00 201.00 196.00 198.00 201.00 189.00 176.00 SPILLED 183.00 189.00 167.00 210.00 186.00 223.00 190.00 231.00 195.00 191.00 209.00 220.00 193.00 201.00 236.00 207.00 218.00 122.00 215.00 194.00 189.00 205.00 199.00 203.00 206.00 196.00 216.00 97.00 218.00 215.00 192.00 195.00 165.00 206.00 152.00 220.00 188.00 227.00 191.00 193.00 202.00 240.00 179.00 196.00 216.00 172.00 221.00 113.00 205.00 211.00 150.00 196.00 218.00 195.00 202.00 196.00 195.00 104.00 1398 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 54 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92681 C92682 C92683 C92685 C92686 C92687 C92688 C92689 C92690 175.00 175.00 184.00 178.00 184.00 148.00 195.00 154.00 203.00 186.00 180.00 193.00 184.00 192.00 211.00 SPILLED 177.00 207.00 185.00 177.00 190.00 179.00 187.00 184.00 SPILLED 171.00 205.00 176.00 180.00 191.00 187.00 196.00 157.00 SPILLED 169.00 225.00 183.00 179.00 192.00 177.00 192.00 181.00 SPILLED 155.00 224.00 195.00 190.00 178.00 201.00 215.00 175.00 SPILLED NOT TAKEN 234.00 193.00 198.00 191.00 204.00 227.00 178.00 197.00 231.00 193.00 190.00 217.00 203.00 231.00 120.00 184.00 237.00 185.00 196.00 216.00 196.00 222.00 48.00 179.00 233.00 178.00 193.00 206.00 132.00 226.00 NOT TAKEN SPILLED 235.00 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 177.00 158.00 174.00 177.00 175.00 188.00 193.00 178.00 168.00 184.00 169.00 167.00 197.00 172.00 185.00 172.00 154.00 161.00 152.00 173.00 169.00 162.00 178.00 178.00 178.00 183.00 188.00 161.00 174.00 176.00 171.00 209.00 172.00 198.00 192.00 161.00 165.00 174.00 177.00 134.00 179.00 172.00 169.00 177.00 191.00 191.00 170.00 177.00 179.00 168.00 184.00 169.00 196.00 201.00 167.00 173.00 173.00 179.00 139.00 178.00 172.00 171.00 187.00 192.00 197.00 160.00 167.00 162.00 165.00 198.00 176.00 193.00 203.00 164.00 169.00 179.00 171.00 NOT TAKEN 175.00 174.00 171.00 186.00 183.00 199.00 167.00 168.00 35.00 169.00 203.00 175.00 192.00 198.00 164.00 168.00 162.00 172.00 177.00 180.00 164.00 189.00 193.00 205.00 173.00 178.00 177.00 210.00 182.00 185.00 209.00 168.00 157.00 167.00 197.00 185.00 191.00 190.00 192.00 194.00 205.00 192.00 SPILLED 197.00 65.00 189.00 195.00 238.00 191.00 164.00 187.00 180.00 185.00 182.00 185.00 199.00 111.00 221.00 175.00 SPILLED 192.00 178.00 187.00 205.00 174.00 170.00 174.00 186.00 181.00 194.00 183.00 180.00 224.00 176.00 181.00 190.00 189.00 194.00 233.00 183.00 187.00 171.00 181.00 176.00 195.00 185.00 197.00 219.00 185.00 176.00 198.00 201.00 192.00 246.00 181.00 172.00 164.00 1399 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 55 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92710 C92711 C92712 C92716 C92717 C92720 C92721 C92723 C92724 C92725 C92726 C92727 C92729 C92730 C92732 C92733 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92745 C92746 C92747 C92748 C92749 C92750 173.00 168.00 202.00 185.00 191.00 200.00 160.00 220.00 217.00 160.00 181.00 183.00 215.00 178.00 190.00 193.00 180.00 178.00 172.00 191.00 170.00 162.00 244.00 189.00 213.00 231.00 183.00 171.00 209.00 161.00 184.00 180.00 171.00 220.00 262.00 195.00 209.00 177.00 SPILLED 214.00 159.00 182.00 194.00 216.00 171.00 187.00 201.00 184.00 185.00 184.00 178.00 184.00 173.00 SPILLED 195.00 213.00 219.00 199.00 190.00 228.00 179.00 179.00 184.00 175.00 227.00 SPILLED 198.00 205.00 190.00 SPILLED 237.00 151.00 184.00 189.00 221.00 177.00 188.00 186.00 188.00 185.00 179.00 173.00 174.00 174.00 SPILLED 199.00 218.00 227.00 185.00 179.00 228.00 178.00 175.00 182.00 177.00 216.00 205.00 197.00 203.00 191.00 SPILLED 224.00 142.00 184.00 205.00 217.00 182.00 184.00 195.00 183.00 190.00 183.00 174.00 178.00 173.00 SPILLED 196.00 211.00 216.00 194.00 178.00 SPILLED 167.00 184.00 180.00 170.00 218.00 SPILLED 201.00 195.00 173.00 SPILLED 214.00 NOT TAKEN 187.00 189.00 215.00 175.00 183.00 195.00 181.00 178.00 181.00 167.00 173.00 179.00 SPILLED 193.00 209.00 225.00 192.00 179.00 SPILLED 166.00 170.00 194.00 180.00 247.00 SPILLED 205.00 207.00 180.00 SPILLED 208.00 185.00 204.00 237.00 184.00 186.00 203.00 194.00 190.00 182.00 184.00 186.00 183.00 SPILLED 198.00 235.00 234.00 207.00 193.00 228.00 171.00 192.00 190.00 180.00 246.00 SPILLED 213.00 194.00 201.00 259.00 233.00 176.00 209.00 216.00 196.00 209.00 207.00 203.00 209.00 202.00 176.00 186.00 182.00 SPILLED 207.00 221.00 244.00 204.00 195.00 231.00 170.00 186.00 182.00 189.00 217.00 119.00 216.00 211.00 196.00 208.00 222.00 181.00 199.00 186.00 198.00 193.00 198.00 193.00 196.00 192.00 181.00 180.00 176.00 SPILLED 203.00 199.00 234.00 201.00 191.00 SPILLED 170.00 188.00 184.00 190.00 SPILLED 402.00 221.00 212.00 195.00 243.00 235.00 188.00 198.00 141.00 190.00 193.00 207.00 184.00 197.00 192.00 191.00 188.00 189.00 SPILLED 210.00 224.00 245.00 224.00 189.00 SPILLED 183.00 192.00 177.00 243.00 SPILLED 230.00 216.00 196.00 252.00 222.00 198.00 197.00 123.00 200.00 198.00 208.00 197.00 185.00 192.00 195.00 187.00 197.00 177.00 196.00 238.00 231.00 215.00 192.00 160.00 176.00 190.00 1400 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 56 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92751 C92753 C92754 C92755 C92756 C92757 C92758 C92760 C92761 C92762 C92763 C92764 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92778 C92780 C92781 C92782 C92783 C92784 C92785 C92786 162.00 188.00 156.00 166.00 213.00 174.00 158.00 184.00 186.00 192.00 223.00 211.00 184.00 257.00 201.00 192.00 195.00 184.00 203.00 172.00 173.00 205.00 176.00 182.00 190.00 165.00 176.00 191.00 188.00 175.00 188.00 188.00 200.00 161.00 176.00 228.00 SPILLED 167.00 191.00 187.00 188.00 221.00 227.00 178.00 252.00 204.00 202.00 207.00 194.00 193.00 174.00 179.00 215.00 185.00 168.00 196.00 170.00 179.00 194.00 191.00 176.00 188.00 202.00 201.00 159.00 176.00 218.00 SPILLED 158.00 194.00 186.00 191.00 223.00 222.00 181.00 SPILLED 194.00 199.00 202.00 182.00 197.00 169.00 165.00 153.00 184.00 181.00 197.00 177.00 179.00 200.00 190.00 181.00 185.00 190.00 135.00 165.00 178.00 226.00 200.00 141.00 194.00 187.00 199.00 225.00 SPILLED 166.00 SPILLED 202.00 202.00 209.00 172.00 187.00 174.00 168.00 217.00 180.00 177.00 197.00 172.00 184.00 192.00 199.00 178.00 190.00 184.00 190.00 163.00 176.00 216.00 SPILLED 112.00 190.00 179.00 196.00 218.00 220.00 159.00 235.00 206.00 193.00 187.00 188.00 NOT TAKEN 167.00 178.00 213.00 177.00 135.00 203.00 168.00 179.00 195.00 189.00 176.00 180.00 190.00 218.00 211.00 95.00 195.00 185.00 196.00 233.00 223.00 180.00 250.00 212.00 225.00 224.00 204.00 166.00 183.00 198.00 197.00 192.00 177.00 188.00 218.00 212.00 193.00 206.00 194.00 218.00 SPILLED 89.00 209.00 180.00 201.00 236.00 231.00 201.00 SPILLED 209.00 211.00 226.00 225.00 170.00 182.00 224.00 198.00 215.00 180.00 208.00 224.00 201.00 201.00 206.00 206.00 205.00 SPILLED 196.00 186.00 208.00 213.00 215.00 180.00 297.00 198.00 199.00 209.00 202.00 166.00 169.00 226.00 165.00 210.00 194.00 199.00 209.00 221.00 187.00 204.00 207.00 200.00 226.00 132.00 213.00 196.00 243.00 175.00 195.00 SPILLED 202.00 213.00 209.00 208.00 178.00 183.00 228.00 199.00 232.00 200.00 204.00 222.00 217.00 203.00 216.00 212.00 225.00 209.00 69.00 200.00 208.00 235.00 84.00 183.00 279.00 201.00 215.00 207.00 206.00 167.00 176.00 218.00 190.00 223.00 22.00 200.00 197.00 237.00 206.00 203.00 1401 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 57 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92787 C92788 C92790 C92791 C92792 C92794 C92795 C92796 C92797 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 209.00 188.00 164.00 190.00 168.00 166.00 176.00 161.00 198.00 165.00 216.00 187.00 178.00 181.00 162.00 148.00 172.00 162.00 175.00 180.00 NOT TAKEN 188.00 137.00 SPILLED 144.00 220.00 178.00 165.00 204.00 232.00 189.00 172.00 189.00 171.00 159.00 186.00 167.00 197.00 153.00 212.00 185.00 171.00 187.00 176.00 174.00 165.00 152.00 180.00 184.00 201.00 127.00 214.00 155.00 227.00 176.00 166.00 188.00 SPILLED 186.00 165.00 195.00 186.00 166.00 181.00 169.00 205.00 168.00 202.00 206.00 182.00 190.00 186.00 186.00 180.00 155.00 177.00 186.00 201.00 136.00 SPILLED 156.00 243.00 177.00 176.00 126.00 187.00 170.00 196.00 179.00 162.00 182.00 171.00 184.00 158.00 209.00 197.00 173.00 194.00 179.00 163.00 181.00 156.00 179.00 190.00 199.00 136.00 SPILLED 156.00 241.00 187.00 178.00 194.00 173.00 172.00 184.00 173.00 154.00 153.00 136.00 190.00 160.00 184.00 190.00 175.00 183.00 169.00 165.00 164.00 157.00 170.00 182.00 192.00 138.00 SPILLED 150.00 176.00 173.00 172.00 SPILLED 182.00 193.00 191.00 56.00 198.00 159.00 NOT TAKEN 215.00 179.00 184.00 189.00 179.00 190.00 164.00 176.00 192.00 205.00 146.00 SPILLED 169.00 195.00 187.00 207.00 208.00 189.00 196.00 195.00 195.00 184.00 204.00 202.00 193.00 196.00 213.00 213.00 182.00 222.00 188.00 210.00 214.00 201.00 201.00 172.00 189.00 217.00 207.00 148.00 223.00 173.00 193.00 187.00 204.00 180.00 209.00 179.00 204.00 184.00 202.00 185.00 170.00 183.00 204.00 216.00 141.00 TECH SPILL NOT TAKEN 187.00 184.00 217.00 186.00 223.00 184.00 211.00 101.00 197.00 194.00 170.00 183.00 221.00 221.00 138.00 221.00 199.00 190.00 211.00 191.00 219.00 179.00 201.00 NOT TAKEN 189.00 198.00 162.00 188.00 221.00 214.00 146.00 216.00 194.00 186.00 1402 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 58 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 165.00 162.00 179.00 198.00 234.00 189.00 190.00 183.00 208.00 165.00 166.00 158.00 196.00 182.00 165.00 174.00 191.00 183.00 259.00 158.00 159.00 186.00 194.00 140.00 178.00 202.00 216.00 156.00 215.00 SPILLED 182.00 162.00 163.00 179.00 171.00 238.00 187.00 182.00 195.00 190.00 183.00 164.00 160.00 205.00 191.00 169.00 195.00 198.00 182.00 259.00 165.00 151.00 183.00 194.00 127.00 186.00 177.00 212.00 158.00 207.00 SPILLED 189.00 151.00 165.00 186.00 189.00 249.00 192.00 191.00 196.00 231.00 189.00 169.00 161.00 207.00 195.00 172.00 192.00 210.00 189.00 257.00 163.00 164.00 191.00 199.00 143.00 188.00 211.00 210.00 167.00 216.00 SPILLED 190.00 159.00 173.00 189.00 190.00 SPILLED 202.00 189.00 205.00 214.00 192.00 171.00 165.00 220.00 196.00 170.00 185.00 210.00 186.00 SPILLED 167.00 155.00 191.00 196.00 145.00 184.00 210.00 217.00 166.00 203.00 SPILLED 186.00 155.00 174.00 179.00 190.00 SPILLED 191.00 168.00 196.00 SPILLED 141.00 174.00 158.00 198.00 195.00 154.00 184.00 201.00 173.00 238.00 160.00 151.00 175.00 188.00 145.00 174.00 196.00 213.00 152.00 223.00 SPILLED 188.00 179.00 182.00 171.00 213.00 207.00 194.00 208.00 219.00 139.00 168.00 169.00 222.00 208.00 173.00 201.00 211.00 191.00 SPILLED 168.00 173.00 162.00 SPILLED 155.00 187.00 161.00 235.00 179.00 225.00 SPILLED 203.00 164.00 SPILLED 190.00 203.00 283.00 200.00 197.00 229.00 182.00 177.00 174.00 225.00 199.00 180.00 SPILLED 129.00 214.00 265.00 174.00 178.00 196.00 222.00 149.00 191.00 207.00 233.00 153.00 199.00 228.00 206.00 184.00 186.00 189.00 199.00 SPILLED 186.00 217.00 200.00 178.00 169.00 178.00 209.00 194.00 180.00 204.00 10.00 28.00 201.00 175.00 193.00 196.00 221.00 148.00 177.00 223.00 205.00 128.00 110.00 SPILLED 201.00 184.00 170.00 SPILLED 205.00 SPILLED 196.00 229.00 SPILLED SPILLED 180.00 189.00 225.00 200.00 190.00 225.00 SPILLED 177.00 NOT TAKEN 192.00 230.00 160.00 186.00 200.00 232.00 139.00 46.00 198.00 206.00 197.60 127.20 176.80 160.80 288.50 192.30 212.90 233.70 172.60 188.00 217.70 194.40 172.90 215.90 242.90 171.40 199.30 239.10 163.50 194.30 204.60 238.80 133.70 197.20 197.20 1403 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 59 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 183.00 169.00 178.00 154.00 167.00 175.00 173.00 174.00 164.00 184.00 184.00 184.00 177.00 175.00 175.00 173.00 182.00 158.00 188.00 171.00 196.00 179.00 175.00 164.00 178.00 190.00 170.00 190.00 177.00 186.00 156.00 180.00 186.00 191.00 191.00 165.00 172.00 163.00 183.00 190.00 170.00 187.00 181.00 183.00 171.00 194.00 198.00 201.00 212.00 189.00 SPILLED 202.00 189.00 170.00 201.00 205.00 203.00 126.00 184.00 170.00 198.00 163.00 179.00 177.00 187.00 200.00 SPILLED 187.00 SPILLED 149.00 120.00 179.00 NOT TAKEN 207.00 217.00 120.00 195.00 SPILLED 191.00 129.00 179.00 201.10 218.80 186.40 200.80 204.70 NOT TAKEN 189.70 GROUP: FEMALE 1 - 0 PPM C92862 C92863 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92876 C92877 C92878 C92879 C92881 C92882 124.00 114.00 156.00 90.00 125.00 131.00 SPILLED 143.00 130.00 SPILLED 121.00 130.00 133.00 109.00 148.00 SPILLED 145.00 143.00 117.00 131.00 132.00 141.00 116.00 158.00 132.00 SPILLED 148.00 122.00 SPILLED 138.00 139.00 154.00 120.00 118.00 SPILLED 149.00 145.00 121.00 124.00 108.00 139.00 111.00 138.00 127.00 SPILLED 147.00 124.00 SPILLED 124.00 137.00 139.00 122.00 132.00 SPILLED 145.00 145.00 131.00 123.00 125.00 138.00 147.00 138.00 132.00 147.00 135.00 127.00 SPILLED 124.00 128.00 143.00 132.00 132.00 155.00 140.00 119.00 119.00 124.00 133.00 138.00 122.00 143.00 131.00 SPILLED 142.00 129.00 167.00 137.00 139.00 144.00 109.00 126.00 SPILLED 139.00 106.00 131.00 132.00 150.00 119.00 149.00 149.00 SPILLED 171.00 134.00 139.00 139.00 134.00 175.00 135.00 162.00 152.00 146.00 108.00 135.00 156.00 134.00 138.00 139.00 122.00 143.00 144.00 128.00 134.00 144.00 128.00 152.00 136.00 167.00 135.00 117.00 137.00 NOT TAKEN 127.00 134.00 142.00 SPILLED 149.00 149.00 152.00 148.00 139.00 138.00 145.00 156.00 150.00 145.00 127.00 145.00 127.00 147.00 144.00 166.00 152.00 154.00 141.00 152.00 153.00 153.00 131.00 170.00 161.00 152.00 135.00 128.00 126.00 149.00 149.00 146.00 139.00 152.00 142.00 125.00 140.00 133.00 142.00 189.00 134.00 151.00 153.00 1404 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 60 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92883 C92884 C92885 C92886 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92899 C92900 C92901 C92902 C92904 C92906 C92907 C92908 C92909 C92910 C92911 C92912 C92913 C92915 C92916 C92917 C92918 137.00 138.00 130.00 143.00 164.00 147.00 117.00 128.00 167.00 115.00 128.00 123.00 128.00 135.00 176.00 143.00 117.00 162.00 109.00 118.00 155.00 111.00 121.00 138.00 TECH SPILL 149.00 115.00 SPILLED 154.00 153.00 136.00 170.00 146.00 139.00 149.00 153.00 101.00 134.00 132.00 147.00 113.00 132.00 113.00 137.00 144.00 171.00 151.00 119.00 160.00 143.00 122.00 105.00 135.00 114.00 SPILLED 108.00 132.00 99.00 169.00 180.00 170.00 128.00 150.00 139.00 119.00 145.00 165.00 104.00 130.00 133.00 156.00 128.00 130.00 123.00 134.00 151.00 168.00 145.00 123.00 161.00 142.00 120.00 112.00 129.00 117.00 174.00 114.00 124.00 107.00 154.00 155.00 158.00 125.00 137.00 143.00 141.00 145.00 150.00 133.00 132.00 135.00 172.00 83.00 129.00 119.00 131.00 144.00 166.00 153.00 114.00 153.00 122.00 119.00 112.00 132.00 120.00 SPILLED 119.00 179.00 119.00 144.00 158.00 149.00 135.00 121.00 141.00 137.00 119.00 167.00 106.00 158.00 130.00 144.00 129.00 145.00 110.00 121.00 151.00 135.00 163.00 118.00 169.00 105.00 112.00 112.00 134.00 107.00 150.00 124.00 170.00 113.00 159.00 160.00 160.00 133.00 160.00 145.00 140.00 154.00 178.00 143.00 146.00 153.00 127.00 126.00 132.00 168.00 165.00 131.00 187.00 133.00 133.00 SPILLED 150.00 113.00 106.00 184.00 123.00 165.00 141.00 137.00 163.00 150.00 153.00 141.00 157.00 155.00 147.00 188.00 120.00 141.00 TECH SPILL 180.00 175.00 131.00 171.00 151.00 121.00 121.00 143.00 122.00 120.00 177.00 125.00 136.00 194.00 162.00 132.00 136.00 146.00 140.00 186.00 157.00 137.00 159.00 124.00 117.00 148.00 145.00 179.00 116.00 186.00 157.00 126.00 133.00 136.00 120.00 117.00 169.00 133.00 160.00 206.00 168.00 143.00 156.00 157.00 96.00 179.00 137.00 139.00 164.00 136.00 136.00 149.00 149.00 173.00 155.00 196.00 144.00 142.00 116.00 152.00 128.00 127.00 195.00 130.00 165.00 180.00 125.00 133.00 141.00 150.00 124.00 SPILLED 147.00 137.00 172.00 154.00 136.00 148.00 144.00 NOT TAKEN 129.00 197.00 137.00 122.00 117.00 144.00 120.00 126.00 178.00 130.00 149.00 194.00 32.00 1405 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 61 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92920 C92921 C92922 C92923 C92924 C92925 C92927 C92928 C92929 C92930 131.00 151.00 149.00 127.00 132.00 150.00 148.00 152.00 116.00 174.00 137.00 155.00 158.00 133.00 118.00 145.00 166.00 165.00 127.00 188.00 137.00 127.00 155.00 128.00 136.00 156.00 SPILLED 161.00 131.00 188.00 102.00 56.00 135.00 127.00 177.00 154.00 145.00 145.00 50.00 175.00 124.00 16.00 147.00 107.00 133.00 152.00 140.00 161.00 SPILLED 136.00 139.00 SPILLED 155.00 164.00 152.00 165.00 180.00 129.00 162.00 155.00 148.00 163.00 NOT TAKEN 125.00 150.00 171.00 144.00 154.00 143.00 NOT TAKEN 179.00 190.00 148.00 170.00 174.00 159.00 144.00 149.00 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92942 C92943 C92946 C92947 C92948 C92951 C92952 C92953 151.00 75.00 165.00 123.00 119.00 114.00 158.00 142.00 135.00 118.00 153.00 151.00 129.00 119.00 147.00 120.00 135.00 104.00 171.00 SPILLED 162.00 137.00 143.00 108.00 152.00 125.00 139.00 133.00 151.00 150.00 127.00 118.00 150.00 135.00 SPILLED 130.00 150.00 SPILLED 122.00 136.00 113.00 145.00 139.00 126.00 131.00 SPILLED 137.00 124.00 133.00 147.00 120.00 122.00 107.00 149.00 150.00 122.00 131.00 109.00 146.00 141.00 122.00 130.00 176.00 140.00 108.00 107.00 138.00 106.00 124.00 116.00 169.00 161.00 140.00 140.00 115.00 156.00 153.00 128.00 137.00 149.00 107.00 128.00 155.00 115.00 123.00 100.00 180.00 160.00 151.00 149.00 127.00 69.00 155.00 138.00 145.00 172.00 103.00 129.00 173.00 221.00 134.00 107.00 196.00 186.00 137.00 160.00 115.00 120.00 151.00 126.00 117.00 141.00 106.00 140.00 140.00 122.00 130.00 115.00 187.00 161.00 95.00 167.00 129.00 72.00 169.00 149.00 145.00 167.00 139.00 131.00 154.00 160.00 TECH SPILL 114.00 160.00 184.00 140.00 177.00 141.00 164.00 147.00 160.00 184.00 141.00 142.00 165.00 128.00 116.00 151.00 143.00 173.00 132.00 153.00 SPILLED 175.00 135.00 136.00 141.00 135.00 114.00 1406 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 62 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92963 C92964 C92965 C92966 C92968 C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92979 C92980 C92981 C92983 C92984 C92985 C92986 C92988 C92989 C92990 142.00 139.00 132.00 128.00 126.00 188.00 189.00 160.00 132.00 132.00 131.00 108.00 155.00 127.00 103.00 134.00 150.00 117.00 134.00 157.00 150.00 116.00 144.00 118.00 127.00 127.00 122.00 114.00 133.00 148.00 160.00 149.00 141.00 128.00 SPILLED 122.00 157.00 140.00 153.00 123.00 SPILLED 149.00 106.00 141.00 138.00 108.00 141.00 151.00 125.00 145.00 162.00 160.00 119.00 135.00 134.00 132.00 138.00 154.00 120.00 150.00 SPILLED 162.00 149.00 131.00 150.00 143.00 122.00 SPILLED 170.00 170.00 141.00 139.00 141.00 110.00 75.00 131.00 99.00 130.00 160.00 111.00 147.00 159.00 140.00 104.00 151.00 132.00 126.00 147.00 145.00 117.00 142.00 159.00 159.00 149.00 140.00 138.00 125.00 122.00 196.00 158.00 146.00 119.00 133.00 146.00 99.00 110.00 134.00 98.00 145.00 152.00 117.00 132.00 135.00 154.00 112.00 135.00 125.00 128.00 137.00 132.00 110.00 152.00 146.00 156.00 135.00 147.00 154.00 127.00 122.00 SPILLED 159.00 170.00 136.00 127.00 158.00 101.00 120.00 144.00 102.00 144.00 152.00 119.00 139.00 173.00 151.00 99.00 140.00 129.00 105.00 141.00 115.00 109.00 135.00 SPILLED 106.00 156.00 164.00 161.00 155.00 136.00 162.00 152.00 184.00 119.00 133.00 157.00 109.00 98.00 146.00 112.00 157.00 138.00 132.00 142.00 168.00 158.00 133.00 113.00 142.00 141.00 140.00 150.00 119.00 127.00 156.00 149.00 167.00 161.00 146.00 137.00 118.00 61.00 146.00 178.00 141.00 136.00 151.00 114.00 142.00 150.00 104.00 123.00 173.00 123.00 141.00 123.00 151.00 131.00 132.00 143.00 133.00 117.00 140.00 111.00 139.00 154.00 139.00 181.00 165.00 153.00 149.00 115.00 159.00 167.00 176.00 121.00 SPILLED 135.00 129.00 125.00 162.00 113.00 142.00 155.00 127.00 152.00 138.00 137.00 130.00 146.00 147.00 159.00 163.00 135.00 142.00 154.00 157.00 165.00 170.00 158.00 139.00 121.00 138.00 164.00 189.00 137.00 152.00 140.00 76.00 NOT TAKEN 159.00 121.00 149.00 175.00 124.00 168.00 187.00 165.00 129.00 90.00 146.00 134.00 204.00 122.00 148.00 150.00 169.00 169.00 158.00 156.00 128.00 123.00 142.00 134.00 180.00 104.00 146.00 134.00 NOT TAKEN 145.00 102.00 153.00 169.00 133.00 154.00 195.00 167.00 121.00 NOT TAKEN 154.00 172.00 185.00 121.00 140.00 131.00 152.00 1407 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 63 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92994 C92997 C92998 C92999 C93001 C93002 C93003 C93004 C93005 C93007 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93019 C93020 C93021 C93022 C93024 C93025 C93026 C93027 C93028 C93029 141.00 162.00 109.00 120.00 130.00 134.00 149.00 132.00 134.00 110.00 155.00 119.00 SPILLED 161.00 119.00 170.00 108.00 152.00 138.00 139.00 136.00 138.00 154.00 132.00 120.00 129.00 156.00 140.00 123.00 139.00 SPILLED 133.00 151.00 142.00 122.00 38.00 144.00 166.00 137.00 155.00 151.00 127.00 121.00 148.00 164.00 123.00 163.00 123.00 155.00 140.00 131.00 135.00 139.00 167.00 152.00 151.00 138.00 164.00 143.00 131.00 149.00 SPILLED 115.00 144.00 95.00 115.00 TECH SPILL 144.00 136.00 139.00 131.00 138.00 121.00 114.00 SPILLED 115.00 129.00 165.00 113.00 152.00 145.00 129.00 146.00 SPILLED 109.00 139.00 131.00 145.00 139.00 131.00 125.00 143.00 SPILLED 119.00 143.00 120.00 125.00 136.00 157.00 146.00 118.00 142.00 134.00 120.00 133.00 126.00 127.00 175.00 114.00 143.00 137.00 146.00 156.00 149.00 149.00 119.00 146.00 157.00 128.00 140.00 146.00 156.00 130.00 164.00 127.00 122.00 140.00 142.00 152.00 148.00 127.00 142.00 125.00 137.00 138.00 128.00 171.00 121.00 140.00 162.00 125.00 151.00 127.00 136.00 110.00 148.00 150.00 133.00 121.00 154.00 SPILLED 133.00 150.00 125.00 124.00 144.00 147.00 150.00 143.00 144.00 157.00 129.00 142.00 155.00 134.00 183.00 121.00 150.00 145.00 148.00 167.00 156.00 62.00 147.00 142.00 152.00 131.00 145.00 133.00 160.00 125.00 149.00 93.00 119.00 155.00 156.00 168.00 146.00 146.00 145.00 128.00 132.00 143.00 136.00 175.00 133.00 169.00 162.00 150.00 174.00 114.00 50.00 149.00 154.00 169.00 139.00 132.00 136.00 173.00 142.00 150.00 136.00 125.00 155.00 146.00 157.00 146.00 144.00 128.00 136.00 134.00 147.00 136.00 181.00 133.00 161.00 136.00 83.00 SPILLED 38.00 156.00 150.00 134.00 137.00 136.00 161.00 SPILLED 144.00 163.00 132.00 127.00 183.00 157.00 175.00 162.00 97.00 157.00 127.00 133.00 147.00 157.00 167.00 140.00 100.00 108.00 142.00 190.00 162.00 158.00 172.00 104.00 150.00 148.00 SPILLED 144.00 155.00 134.00 120.00 147.00 149.00 159.00 134.00 99.00 150.00 116.00 134.00 161.00 150.00 SPILLED 135.00 77.00 179.00 SPILLED 180.00 106.00 146.00 SPILLED 148.00 147.00 138.00 NOT TAKEN 1408 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 64 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93030 C93032 C93033 C93034 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93046 C93047 C93048 C93049 C93050 139.00 135.00 166.00 124.00 137.00 120.00 126.00 132.00 130.00 151.00 155.00 152.00 138.00 146.00 146.00 CNTMD 122.00 153.00 134.00 146.00 148.00 136.00 159.00 138.00 140.00 146.00 130.00 165.00 158.00 148.00 127.00 157.00 175.00 134.00 120.00 145.00 136.00 135.00 167.00 122.00 167.00 117.00 128.00 135.00 158.00 162.00 165.00 168.00 128.00 151.00 177.00 118.00 115.00 147.00 134.00 155.00 163.00 131.00 171.00 146.00 131.00 132.00 138.00 170.00 166.00 182.00 129.00 155.00 156.00 120.00 136.00 152.00 138.00 145.00 80.00 136.00 163.00 150.00 146.00 163.00 129.00 169.00 154.00 203.00 139.00 128.00 157.00 113.00 123.00 157.00 144.00 148.00 143.00 147.00 151.00 131.00 145.00 147.00 125.00 178.00 194.00 SPILLED 135.00 160.00 190.00 142.00 127.00 159.00 138.00 153.00 SPILLED 147.00 175.00 135.00 130.00 152.00 153.00 171.00 196.00 197.00 58.00 162.00 173.00 141.00 139.00 159.00 143.00 152.00 71.00 152.00 171.00 83.00 127.00 148.00 153.00 174.00 199.00 196.00 58.00 151.00 194.00 138.00 67.00 156.00 163.00 158.00 159.00 174.00 146.00 162.00 SPILLED 175.00 178.00 SPILLED 146.00 196.00 143.00 48.00 163.00 146.00 133.00 138.00 184.00 158.00 158.00 167.00 192.00 SPILLED 133.00 212.00 133.00 143.00 GROUP: FEMALE 4 - 5 PPM C93052 C93053 C93054 C93055 C93056 C93057 C93059 C93060 C93061 C93062 122.00 144.00 140.00 120.00 143.00 119.00 138.00 111.00 154.00 133.00 142.00 139.00 151.00 147.00 142.00 127.00 135.00 144.00 151.00 130.00 133.00 134.00 138.00 142.00 141.00 109.00 128.00 134.00 164.00 126.00 139.00 146.00 149.00 152.00 140.00 126.00 140.00 140.00 163.00 129.00 147.00 155.00 142.00 156.00 135.00 116.00 125.00 162.00 171.00 133.00 139.00 162.00 143.00 146.00 144.00 121.00 137.00 151.00 193.00 132.00 129.00 80.00 125.00 154.00 138.00 124.00 150.00 153.00 194.00 136.00 84.00 78.00 112.00 163.00 147.00 126.00 147.00 140.00 SPILLED 133.00 72.00 142.00 163.00 159.00 142.00 145.00 167.00 210.00 132.00 NOT TAKEN NOT TAKEN 165.00 152.00 135.00 140.00 117.00 160.00 129.00 1409 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 65 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93063 C93065 C93066 C93068 C93069 C93072 C93073 C93074 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93097 C93098 C93099 C93100 C93101 121.00 123.00 SPILLED 132.00 145.00 132.00 132.00 130.00 144.00 141.00 137.00 131.00 SPILLED 108.00 134.00 124.00 132.00 104.00 135.00 123.00 107.00 136.00 140.00 156.00 127.00 118.00 142.00 131.00 139.00 122.00 108.00 121.00 121.00 SPILLED 139.00 155.00 125.00 140.00 137.00 143.00 138.00 130.00 137.00 SPILLED 121.00 138.00 140.00 132.00 113.00 142.00 143.00 114.00 144.00 152.00 156.00 141.00 117.00 137.00 114.00 138.00 159.00 105.00 117.00 121.00 SPILLED 132.00 158.00 126.00 131.00 73.00 152.00 150.00 135.00 162.00 SPILLED 109.00 131.00 141.00 136.00 118.00 126.00 108.00 127.00 136.00 135.00 144.00 128.00 118.00 129.00 116.00 135.00 148.00 128.00 123.00 131.00 160.00 141.00 153.00 141.00 SPILLED 179.00 133.00 142.00 132.00 155.00 140.00 128.00 141.00 135.00 125.00 106.00 135.00 142.00 118.00 147.00 138.00 164.00 127.00 116.00 131.00 110.00 140.00 153.00 132.00 114.00 131.00 SPILLED 134.00 165.00 135.00 184.00 154.00 126.00 121.00 131.00 161.00 SPILLED 125.00 120.00 139.00 127.00 108.00 156.00 134.00 112.00 147.00 139.00 141.00 132.00 114.00 SPILLED 108.00 148.00 141.00 109.00 123.00 126.00 183.00 152.00 154.00 128.00 168.00 156.00 152.00 136.00 199.00 165.00 SPILLED 157.00 131.00 139.00 131.00 118.00 165.00 148.00 123.00 150.00 147.00 154.00 142.00 116.00 127.00 127.00 151.00 155.00 113.00 121.00 133.00 149.00 135.00 154.00 125.00 158.00 151.00 146.00 115.00 138.00 137.00 133.00 137.00 143.00 134.00 138.00 111.00 164.00 134.00 134.00 157.00 134.00 152.00 132.00 117.00 133.00 148.00 151.00 163.00 119.00 116.00 139.00 SPILLED 137.00 156.00 142.00 144.00 153.00 127.00 170.00 146.00 165.00 SPILLED 121.00 147.00 140.00 150.00 115.00 169.00 130.00 142.00 159.00 SPILLED 148.00 151.00 115.00 131.00 130.00 151.00 169.00 114.00 135.00 135.00 SPILLED 144.00 167.00 153.00 148.00 154.00 193.00 171.00 155.00 178.00 159.00 140.00 148.00 159.00 142.00 135.00 111.00 135.00 143.00 166.00 158.00 137.00 143.00 133.00 162.00 132.00 147.00 161.00 113.00 114.00 109.00 SPILLED 151.00 148.00 149.00 54.00 143.00 175.00 169.00 153.00 155.00 SPILLED 122.00 147.00 158.00 157.00 116.00 80.00 143.00 133.00 186.00 66.00 161.00 145.00 122.00 126.00 132.00 143.00 160.00 133.00 1410 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 66 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93110 161.00 173.00 174.00 130.00 117.00 106.00 107.00 183.00 160.00 179.00 150.00 136.00 118.00 122.00 109.00 201.00 167.00 157.00 168.00 137.00 134.00 108.00 99.00 200.00 172.00 169.00 170.00 142.00 125.00 SPILLED 107.00 184.00 169.00 156.00 157.00 139.00 106.00 116.00 NOT TAKEN 213.00 168.00 159.00 173.00 153.00 113.00 109.00 187.00 164.00 167.00 169.00 149.00 138.00 115.00 224.00 196.00 173.00 156.00 159.00 130.00 121.00 181.00 183.00 178.00 123.00 162.00 140.00 122.00 174.00 157.00 169.00 151.00 SPILLED 152.00 116.00 213.00 GROUP: FEMALE 5 - 20 PPM C93112 C93113 C93115 C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93124 C93125 C93126 C93128 C93129 C93130 C93131 C93132 C93133 C93134 127.00 119.00 124.00 126.00 155.00 114.00 108.00 120.00 95.00 145.00 105.00 112.00 117.00 106.00 131.00 126.00 135.00 131.00 108.00 128.00 131.00 137.00 129.00 131.00 161.00 124.00 120.00 131.00 103.00 164.00 111.00 122.00 128.00 125.00 143.00 147.00 130.00 125.00 162.00 123.00 SPILLED 124.00 125.00 130.00 154.00 125.00 116.00 123.00 SPILLED 160.00 130.00 123.00 121.00 126.00 142.00 127.00 137.00 124.00 SPILLED 126.00 144.00 120.00 126.00 134.00 SPILLED 125.00 119.00 123.00 103.00 183.00 123.00 115.00 127.00 121.00 148.00 139.00 138.00 135.00 140.00 133.00 111.00 135.00 128.00 124.00 206.00 116.00 131.00 109.00 107.00 172.00 129.00 117.00 124.00 118.00 152.00 150.00 138.00 109.00 100.00 144.00 129.00 135.00 115.00 126.00 107.00 166.00 125.00 119.00 63.00 115.00 143.00 139.00 125.00 146.00 139.00 124.00 131.00 118.00 120.00 109.00 173.00 129.00 129.00 117.00 139.00 130.00 128.00 104.00 106.00 131.00 140.00 122.00 SPILLED 354.00 170.00 132.00 123.00 137.00 SPILLED 138.00 131.00 129.00 123.00 130.00 146.00 159.00 128.00 118.00 189.00 125.00 128.00 135.00 190.00 157.00 141.00 142.00 NOT TAKEN 139.00 131.00 142.00 138.00 82.00 215.00 128.00 119.00 130.00 161.00 145.00 123.00 129.00 1411 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 67 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93145 C93146 C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93156 C93157 C93158 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93170 C93171 141.00 141.00 128.00 109.00 130.00 161.00 140.00 SPILLED 90.00 106.00 146.00 112.00 147.00 126.00 126.00 121.00 134.00 119.00 124.00 127.00 175.00 146.00 124.00 118.00 106.00 124.00 108.00 120.00 111.00 101.00 132.00 152.00 152.00 125.00 119.00 121.00 166.00 146.00 SPILLED 114.00 114.00 152.00 135.00 141.00 156.00 132.00 133.00 135.00 115.00 137.00 177.00 191.00 124.00 133.00 124.00 116.00 119.00 156.00 124.00 SPILLED 210.00 140.00 146.00 129.00 107.00 116.00 146.00 138.00 SPILLED 102.00 113.00 150.00 125.00 142.00 115.00 133.00 135.00 120.00 114.00 134.00 135.00 195.00 139.00 96.00 107.00 125.00 114.00 101.00 111.00 SPILLED 133.00 147.00 152.00 140.00 118.00 124.00 156.00 134.00 146.00 137.00 106.00 157.00 129.00 149.00 114.00 144.00 141.00 133.00 116.00 123.00 136.00 SPILLED 152.00 124.00 118.00 126.00 119.00 109.00 118.00 209.00 131.00 145.00 159.00 125.00 138.00 118.00 170.00 148.00 SPILLED 110.00 118.00 153.00 127.00 151.00 120.00 162.00 145.00 116.00 109.00 131.00 150.00 SPILLED 154.00 130.00 110.00 132.00 122.00 150.00 136.00 159.00 124.00 171.00 116.00 116.00 169.00 145.00 139.00 117.00 164.00 131.00 144.00 121.00 124.00 133.00 117.00 133.00 139.00 208.00 152.00 141.00 119.00 124.00 119.00 135.00 174.00 SPILLED 144.00 124.00 177.00 136.00 SPILLED 126.00 169.00 138.00 151.00 128.00 121.00 142.00 114.00 135.00 137.00 197.00 143.00 149.00 130.00 126.00 134.00 130.00 SPILLED 182.00 129.00 119.00 180.00 176.00 132.00 118.00 157.00 139.00 157.00 128.00 104.00 148.00 126.00 136.00 135.00 SPILLED 172.00 137.00 126.00 133.00 153.00 145.00 SPILLED 202.00 149.00 117.00 180.00 160.00 123.00 136.00 158.00 158.00 162.00 136.00 168.00 116.00 137.00 143.00 SPILLED 136.00 149.00 141.00 136.00 135.00 142.00 SPILLED SPILLED 154.00 124.00 168.00 159.00 146.00 132.00 158.00 137.00 158.00 132.00 170.00 113.00 132.00 144.00 12.00 185.00 SPILLED 130.00 131.00 138.00 134.00 SPILLED 1412 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 68 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93172 C93173 C93174 C93175 C93176 C93177 C93178 C93179 C93180 122.00 112.00 SPILLED 110.00 128.00 113.00 110.00 112.00 135.00 128.00 126.00 SPILLED 112.00 124.00 114.00 109.00 120.00 143.00 124.00 118.00 SPILLED 111.00 115.00 112.00 99.00 107.00 135.00 131.00 119.00 188.00 110.00 116.00 115.00 113.00 112.00 157.00 126.00 115.00 190.00 113.00 124.00 116.00 107.00 105.00 147.00 148.00 135.00 SPILLED 109.00 134.00 130.00 109.00 120.00 136.00 122.00 125.00 138.00 111.00 121.00 118.00 108.00 110.00 117.00 152.00 SPILLED SPILLED 113.00 131.00 124.00 115.00 114.00 135.00 130.00 162.00 109.00 138.00 138.00 111.00 115.00 52.00 130.00 139.00 115.00 118.00 118.00 113.00 114.00 GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 122.00 110.00 138.00 115.00 130.00 119.00 137.00 134.00 93.00 137.00 174.00 124.00 137.00 133.00 125.00 111.00 97.00 115.00 123.00 128.00 113.00 115.00 119.00 126.00 124.00 145.00 142.00 113.00 146.00 164.00 134.00 165.00 136.00 136.00 115.00 117.00 124.00 124.00 118.00 107.00 106.00 120.00 131.00 145.00 136.00 144.00 150.00 144.00 158.00 124.00 153.00 131.00 134.00 121.00 101.00 129.00 121.00 126.00 113.00 139.00 122.00 129.00 135.00 136.00 141.00 138.00 148.00 146.00 133.00 159.00 126.00 135.00 113.00 120.00 129.00 120.00 139.00 119.00 142.00 121.00 128.00 144.00 145.00 147.00 138.00 145.00 146.00 125.00 155.00 141.00 136.00 123.00 119.00 133.00 119.00 109.00 133.00 131.00 127.00 125.00 148.00 141.00 166.00 117.00 158.00 158.00 123.00 163.00 145.00 144.00 137.00 SPILLED 147.00 143.00 116.00 124.00 137.00 130.00 137.00 155.00 137.00 159.00 135.00 158.00 159.00 147.00 175.00 184.00 147.00 140.00 SPILLED 144.00 137.00 119.00 133.00 129.00 124.00 159.00 150.00 153.00 172.00 135.00 166.00 172.00 137.00 185.00 145.00 152.00 122.00 SPILLED 146.00 67.00 149.00 144.00 164.00 148.00 159.00 159.00 160.00 179.00 126.00 169.00 SPILLED 156.00 185.00 153.00 145.00 135.00 165.00 153.00 134.00 142.00 151.00 154.00 142.00 SPILLED 150.00 161.00 147.00 174.00 206.00 148.00 166.00 150.00 139.00 146.00 162.00 1413 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 69 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93200 C93201 C93202 C93203 C93204 C93205 C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 123.00 103.00 103.00 140.00 117.00 119.00 124.00 122.00 132.00 SPILLED 125.00 106.00 136.00 115.00 126.00 113.00 130.00 146.00 110.00 115.00 133.00 128.00 125.00 97.00 138.00 125.00 120.00 134.00 SPILLED 115.00 SPILLED 138.00 111.00 155.00 121.00 132.00 120.00 130.00 147.00 122.00 130.00 148.00 122.00 109.00 99.00 136.00 126.00 115.00 136.00 126.00 SPILLED 139.00 105.00 150.00 96.00 131.00 119.00 132.00 134.00 115.00 136.00 149.00 123.00 146.00 103.00 139.00 138.00 128.00 139.00 129.00 SPILLED 139.00 111.00 146.00 96.00 125.00 112.00 130.00 135.00 116.00 127.00 143.00 128.00 110.00 95.00 162.00 135.00 126.00 132.00 114.00 SPILLED 153.00 106.00 145.00 143.00 131.00 125.00 137.00 134.00 127.00 128.00 143.00 136.00 111.00 103.00 175.00 152.00 123.00 148.00 118.00 SPILLED SPILLED SPILLED 178.00 136.00 148.00 164.00 152.00 158.00 137.00 151.00 156.00 142.00 133.00 120.00 162.00 143.00 137.00 157.00 134.00 SPILLED 161.00 120.00 177.00 135.00 153.00 146.00 150.00 161.00 127.00 165.00 135.00 136.00 SPILLED 105.00 162.00 123.00 140.00 155.00 160.00 168.00 168.00 154.00 131.00 112.00 149.00 138.00 164.00 151.00 135.00 161.00 155.00 141.00 139.00 129.00 180.00 134.00 152.00 168.00 171.00 176.00 192.00 156.00 181.00 156.00 157.00 159.00 157.00 171.00 138.00 170.00 153.00 132.00 133.00 144.00 169.00 130.00 158.00 159.00 147.00 156.00 SPILLED 140.00 181.00 148.00 171.00 154.00 151.00 154.00 121.00 159.00 142.00 1414 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 70 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: C92502 C92503 C92505 C92508 C92510 C92513 C92514 C92515 C92518 C92519 C92520 C92522 C92527 C92530 C92533 C92535 C92536 C92537 C92539 C92541 C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92554 C92557 MALE 1 - 0 PPM 50.00 159.00 131.00 194.00 189.00 27.00 193.00 200.00 181.00 185.00 211.00 220.00 146.00 176.00 217.00 231.00 198.00 NOT TAKEN 168.00 61.00 177.00 166.00 183.00 56.00 112.00 205.00 192.00 179.00 198.00 195.00 222.00 237.00 195.00 210.00 224.00 220.00 203.00 199.00 187.00 192.00 187.00 172.00 211.00 219.00 201.00 171.00 115.00 82.00 168.00 NOT TAKEN SPILLED 223.00 187.00 190.00 208.00 211.00 168.00 182.00 199.00 164.00 147.00 205.00 196.00 180.00 214.00 178.00 234.00 181.00 NOT TAKEN 142.00 187.00 230.00 195.00 224.00 191.00 187.00 182.00 227.00 159.00 210.00 194.00 187.00 176.00 88.00 126.00 187.00 207.00 168.00 175.00 175.00 242.00 169.00 191.00 231.00 161.00 229.00 198.00 187.00 177.00 230.00 NOT TAKEN 225.00 188.00 181.00 184.00 19.00 155.00 195.00 196.00 187.00 SPILLED 170.00 228.00 160.00 190.00 208.00 164.00 220.00 198.00 186.00 184.00 208.00 198.00 194.00 124.00 174.00 170.00 167.00 200.00 188.00 244.00 174.00 SPILLED 160.00 172.00 163.00 SPILLED 179.00 242.00 263.00 177.00 152.00 193.00 177.00 214.00 SPILLED NOT TAKEN 21.00 219.00 203.00 188.00 SPILLED 195.00 182.00 NOT TAKEN 152.00 140.00 180.00 104.00 247.00 185.00 112.00 180.00 225.00 191.00 40.00 140.00 NOT TAKEN 169.00 NOT TAKEN 1415 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 71 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 1 - 0 PPM C92558 C92559 C92560 C92563 C92565 C92567 C92568 C92569 106.00 191.00 166.00 SPILLED 204.00 190.00 184.00 174.00 NOT TAKEN NOT TAKEN 219.00 205.00 209.00 184.00 178.00 NOT TAKEN 198.00 183.00 195.00 179.00 175.00 217.00 192.00 178.00 177.00 184.00 167.00 176.00 139.00 164.00 167.00 GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92587 C92588 C92589 C92590 C92591 C92592 C92594 C92595 C92597 206.00 196.00 167.00 209.00 191.00 191.00 143.00 44.00 240.00 162.00 174.00 202.00 185.00 218.00 200.00 190.00 188.00 195.00 148.00 163.00 230.00 199.00 92.00 188.00 171.00 162.00 103.00 193.00 139.00 163.00 178.00 170.00 177.00 189.00 186.00 181.00 183.00 130.00 177.00 221.00 200.00 NOT TAKEN 207.00 197.00 71.00 108.00 229.00 169.00 178.00 105.00 192.00 179.00 202.00 180.00 202.00 194.00 NOT TAKEN 178.00 237.00 203.00 208.00 199.00 205.00 167.00 184.00 NOT TAKEN 196.00 175.00 210.00 189.00 NOT TAKEN 197.00 139.00 230.00 197.00 219.00 200.00 122.00 195.00 121.00 193.00 SPILLED 58.00 183.00 170.00 169.00 170.00 178.00 211.00 145.00 213.00 175.00 193.00 168.00 107.00 147.00 188.00 178.00 98.00 205.00 NOT TAKEN NOT TAKEN 168.00 197.00 SPILLED 158.00 83.00 NOT TAKEN 229.00 170.00 1.00 133.00 223.00 SPILLED 1416 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 72 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92598 C92601 C92602 C92604 C92605 C92606 C92607 C92608 C92609 C92611 C92612 C92614 C92615 C92617 C92619 C92620 C92622 C92623 C92625 C92626 C92629 C92630 177.00 187.00 186.00 103.00 187.00 189.00 221.00 23.00 187.00 177.00 34.00 SPILLED 185.00 134.00 165.00 172.00 207.00 156.00 168.00 178.00 101.00 204.00 184.00 204.00 218.00 118.00 197.00 211.00 201.00 165.00 SPILLED 174.00 68.00 172.00 162.00 178.00 151.00 104.00 187.00 154.00 190.00 197.00 193.00 106.00 197.00 91.00 200.00 166.00 SPILLED 161.00 175.00 205.00 160.00 NOT TAKEN 199.00 67.00 181.00 184.00 188.00 90.00 194.00 82.00 200.00 NOT TAKEN SPILLED 50.00 178.00 188.00 164.00 SPILLED NOT TAKEN 183.00 203.00 177.00 171.00 198.00 NOT TAKEN 192.00 117.00 162.00 SPILLED 190.00 204.00 178.00 126.00 208.00 175.00 NOT TAKEN 161.00 201.00 181.00 203.00 167.00 133.00 202.00 192.00 165.00 SPILLED 169.00 186.00 181.00 NOT TAKEN NOT TAKEN 181.00 175.00 158.00 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 182.00 195.00 202.00 197.00 28.00 SPILLED 189.00 180.00 197.00 202.00 SPILLED 193.00 181.00 197.00 201.00 199.00 184.00 173.00 183.00 SPILLED 201.00 SPILLED 199.00 SPILLED 205.00 186.00 219.00 SPILLED 199.00 187.00 209.00 199.00 250.00 1417 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 73 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92637 C92638 C92639 C92641 C92642 C92643 C92644 C92647 C92648 C92649 C92651 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92663 C92664 C92665 C92666 C92667 C92669 C92671 C92672 C92673 C92674 C92675 186.00 211.00 99.00 162.00 164.00 196.00 185.00 162.00 147.00 188.00 92.00 213.00 95.00 216.00 195.00 189.00 191.00 198.00 199.00 203.00 236.00 69.00 212.00 NOT TAKEN 5.00 191.00 203.00 202.00 215.00 209.00 191.00 180.00 194.00 194.00 162.00 166.00 190.00 188.00 NOT TAKEN 141.00 SPILLED 42.00 215.00 212.00 186.00 177.00 212.00 197.00 180.00 210.00 226.00 211.00 201.00 188.00 198.00 219.00 204.00 188.00 185.00 122.00 36.00 172.00 181.00 201.00 182.00 SPILLED 188.00 220.00 211.00 201.00 196.00 211.00 SPILLED 198.00 15.00 218.00 SPILLED 211.00 235.00 226.00 213.00 111.00 189.00 191.00 116.00 165.00 172.00 171.00 196.00 191.00 180.00 184.00 179.00 201.00 202.00 202.00 171.00 184.00 195.00 199.00 154.00 183.00 114.00 240.00 NOT TAKEN 223.00 213.00 123.00 89.00 210.00 180.00 172.00 SPILLED 213.00 206.00 213.00 204.00 196.00 196.00 179.00 216.00 188.00 164.00 173.00 198.00 160.00 179.00 199.00 184.00 199.00 187.00 204.00 207.00 33.00 203.00 SPILLED 202.00 225.00 214.00 130.00 194.00 SPILLED 194.00 181.00 185.00 201.00 206.00 204.00 SPILLED 199.00 225.00 211.00 81.00 219.00 83.00 194.00 188.00 148.00 201.00 105.00 192.00 SPILLED 201.00 178.00 205.00 1418 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 74 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92677 C92680 C92681 C92682 C92683 C92685 C92686 C92688 C92690 199.00 143.00 182.00 197.00 203.00 132.00 238.00 SPILLED 236.00 177.00 105.00 164.00 215.00 217.00 164.00 SPILLED 233.00 190.00 NOT TAKEN 148.00 202.00 213.00 200.00 231.00 217.00 SPILLED 203.00 SPILLED 247.00 202.00 224.00 211.00 89.00 229.00 204.00 203.00 213.00 91.00 228.00 230.00 207.00 224.00 SPILLED 243.00 SPILLED GROUP: MALE 4 - 5 PPM C92691 C92693 C92694 C92695 C92696 C92698 C92699 C92700 C92702 C92704 C92705 C92706 C92707 C92708 C92709 C92711 C92712 C92716 C92717 173.00 SPILLED 190.00 174.00 173.00 216.00 184.00 158.00 191.00 187.00 193.00 231.00 175.00 173.00 150.00 170.00 210.00 SPILLED 228.00 178.00 182.00 197.00 183.00 210.00 216.00 176.00 175.00 195.00 190.00 183.00 SPILLED 177.00 155.00 131.00 187.00 169.00 SPILLED 214.00 179.00 187.00 204.00 180.00 211.00 232.00 171.00 188.00 205.00 204.00 191.00 214.00 182.00 195.00 98.00 199.00 132.00 SPILLED 224.00 188.00 192.00 201.00 191.00 215.00 231.00 170.00 173.00 197.00 201.00 193.00 250.00 27.00 203.00 107.00 97.00 SPILLED 231.00 171.00 194.00 207.00 179.00 206.00 228.00 170.00 203.00 208.00 198.00 198.00 SPILLED 192.00 NOT TAKEN SPILLED 219.00 187.00 159.00 210.00 190.00 218.00 215.00 163.00 187.00 217.00 209.00 205.00 174.00 197.00 186.00 148.00 206.00 192.00 216.00 143.00 181.00 154.00 135.00 197.00 224.00 194.00 165.00 156.00 206.00 199.00 217.00 15.00 175.00 120.00 106.00 187.00 220.00 209.00 167.00 186.00 SPILLED 174.00 SPILLED 177.00 1419 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 75 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92720 C92721 C92723 C92724 C92726 C92727 C92729 C92730 C92732 C92733 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92745 C92746 C92747 C92748 C92749 C92750 217.00 213.00 SPILLED 214.00 194.00 193.00 106.00 196.00 189.00 186.00 198.00 196.00 192.00 207.00 186.00 176.00 SPILLED SPILLED 245.00 241.00 235.00 195.00 SPILLED 179.00 198.00 173.00 NOT TAKEN 197.00 192.00 183.00 191.00 185.00 191.00 194.00 190.00 192.00 217.00 188.00 201.00 SPILLED 198.00 234.00 212.00 181.00 247.00 184.00 188.00 219.00 191.00 183.00 198.00 88.00 201.00 198.00 179.00 210.00 195.00 196.00 144.00 190.00 NOT TAKEN 105.00 193.00 235.00 191.00 197.00 231.00 200.00 126.00 190.00 22.00 198.00 190.00 68.00 260.00 188.00 199.00 29.00 195.00 50.00 SPILLED 160.00 183.00 197.00 228.00 195.00 235.00 SPILLED 193.00 152.00 190.00 184.00 NOT TAKEN 197.00 179.00 210.00 195.00 189.00 207.00 197.00 211.00 SPILLED 218.00 186.00 187.00 SPILLED 189.00 198.00 202.00 215.00 225.00 114.00 196.00 220.00 203.00 210.00 210.00 SPILLED 184.00 196.00 183.00 229.00 219.00 89.00 198.00 201.00 172.00 211.00 187.00 CNTMD 121.00 98.00 GROUP: MALE 5 - 20 PPM C92753 C92756 C92757 214.00 224.00 218.00 197.00 210.00 194.00 229.00 86.00 201.00 228.00 NOT TAKEN 203.00 220.00 176.00 234.00 152.00 187.00 159.00 159.00 100.00 1420 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 76 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92761 C92762 C92763 C92764 C92766 C92767 C92768 C92769 C92770 C92771 C92773 C92774 C92775 C92776 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92788 C92791 C92792 C92797 C92800 C92802 C92803 C92804 C92806 C92807 188.00 193.00 SPILLED NOT TAKEN 197.00 190.00 207.00 208.00 215.00 204.00 180.00 180.00 213.00 191.00 SPILLED 43.00 212.00 120.00 134.00 190.00 206.00 209.00 205.00 196.00 205.00 184.00 217.00 175.00 203.00 192.00 198.00 194.00 176.00 214.00 168.00 250.00 204.00 218.00 SPILLED 26.00 190.00 167.00 218.00 178.00 222.00 219.00 NOT TAKEN 202.00 201.00 193.00 199.00 189.00 190.00 171.00 215.00 169.00 199.00 196.00 138.00 176.00 182.00 229.00 181.00 254.00 206.00 215.00 SPILLED 169.00 183.00 232.00 199.00 SPILLED 206.00 204.00 201.00 215.00 214.00 209.00 223.00 179.00 223.00 178.00 222.00 180.00 102.00 200.00 193.00 219.00 165.00 297.00 203.00 169.00 219.00 176.00 196.00 217.00 202.00 232.00 215.00 188.00 205.00 211.00 199.00 190.00 184.00 165.00 204.00 176.00 209.00 203.00 179.00 192.00 68.00 176.00 228.00 163.00 213.00 207.00 188.00 184.00 209.00 206.00 238.00 210.00 194.00 209.00 203.00 204.00 186.00 38.00 158.00 209.00 184.00 200.00 185.00 207.00 185.00 181.00 50.00 197.00 215.00 187.00 183.00 191.00 198.00 188.00 254.00 167.00 206.00 208.00 210.00 186.00 171.00 223.00 175.00 202.00 157.00 252.00 170.00 167.00 140.00 99.00 201.00 199.00 166.00 190.00 125.00 236.00 SPILLED 162.00 183.00 174.00 42.00 202.00 69.00 214.00 215.00 202.00 203.00 183.00 155.00 225.00 182.00 228.00 212.00 215.00 210.00 144.00 153.00 199.00 192.00 231.00 1421 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 77 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92808 C92809 C92810 C92813 C92814 C92815 C92818 C92819 175.00 186.00 220.00 224.00 142.00 SPILLED 179.00 184.00 171.00 191.00 216.00 188.00 153.00 214.00 181.00 188.00 175.00 204.00 231.00 208.00 142.00 SPILLED 189.00 203.00 181.00 202.00 153.00 178.00 148.00 227.00 199.00 205.00 180.00 221.00 91.00 201.00 157.00 SPILLED 194.00 225.00 188.00 238.00 31.00 159.00 151.00 SPILLED 193.00 SPILLED 178.00 207.00 NOT TAKEN 146.00 252.00 180.00 219.00 175.00 173.00 158.00 249.00 189.00 NOT TAKEN GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92827 C92828 C92829 C92830 C92831 C92833 C92834 C92836 C92839 C92840 C92842 C92843 C92844 C92845 C92846 198.00 98.00 187.00 84.00 263.00 192.00 196.00 220.00 139.00 148.00 217.00 202.00 196.00 249.00 164.00 201.00 212.00 165.00 174.00 155.00 190.00 76.00 198.00 215.00 229.00 206.00 194.00 204.00 130.00 192.00 202.00 187.00 205.00 SPILLED 175.00 179.00 120.00 188.00 167.00 187.00 197.00 165.00 257.00 201.00 SPILLED 126.00 117.00 217.00 180.00 195.00 184.00 181.00 195.00 184.00 181.00 203.00 205.00 210.00 226.00 202.00 SPILLED SPILLED 174.00 196.00 181.00 111.00 188.00 180.00 191.00 240.00 178.00 167.00 206.00 223.00 209.00 SPILLED 99.00 SPILLED 186.00 199.00 206.00 210.00 172.00 186.00 194.00 SPILLED 168.00 SPILLED 192.00 190.00 178.00 197.00 217.00 223.00 SPILLED SPILLED 175.00 71.00 155.00 173.00 209.00 217.00 98.00 122.00 184.00 1422 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 78 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92847 C92848 C92850 C92851 C92853 C92854 C92856 C92857 C92858 C92860 256.00 161.00 182.00 194.00 209.00 208.00 181.00 197.00 202.00 197.00 245.00 154.00 207.00 204.00 209.00 218.00 187.00 106.00 191.00 194.00 218.00 92.00 197.00 224.00 209.00 212.00 183.00 210.00 184.00 218.00 144.00 181.00 187.00 204.00 207.00 134.00 203.00 188.00 151.00 SPILLED 159.00 180.00 203.00 221.00 194.00 58.00 193.00 89.00 SPILLED 115.00 170.00 205.00 204.00 180.00 60.00 74.00 SPILLED 132.00 205.00 177.00 155.00 160.00 SPILLED 62.00 197.00 108.00 178.00 GROUP: FEMALE 1 - 0 PPM C92863 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92877 C92878 C92879 C92881 C92882 C92883 C92884 110.00 134.00 144.00 143.00 161.00 139.00 142.00 158.00 144.00 155.00 35.00 127.00 150.00 132.00 141.00 134.00 143.00 139.00 121.00 139.00 150.00 159.00 146.00 159.00 155.00 159.00 159.00 160.00 137.00 203.00 50.00 141.00 127.00 131.00 140.00 132.00 154.00 159.00 181.00 168.00 170.00 179.00 140.00 160.00 145.00 114.00 156.00 152.00 79.00 102.00 136.00 130.00 156.00 140.00 159.00 166.00 147.00 160.00 163.00 154.00 SPILLED 155.00 139.00 97.00 144.00 141.00 153.00 SPILLED 117.00 161.00 147.00 156.00 120.00 SPILLED 147.00 141.00 SPILLED 120.00 148.00 169.00 141.00 110.00 170.00 164.00 156.00 156.00 139.00 108.00 NOT TAKEN 92.00 147.00 163.00 144.00 108.00 175.00 143.00 159.00 158.00 152.00 156.00 83.00 150.00 174.00 147.00 25.00 179.00 149.00 111.00 130.00 142.00 127.00 1423 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 79 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 1 - 0 PPM C92885 C92886 C92888 C92890 C92891 C92892 C92893 C92895 C92896 C92897 C92901 C92902 C92904 C92906 C92907 C92908 C92909 C92911 C92912 C92913 C92915 C92916 C92924 C92925 C92927 C92928 152.00 154.00 139.00 172.00 117.00 166.00 140.00 129.00 129.00 152.00 46.00 202.00 140.00 117.00 119.00 151.00 126.00 117.00 174.00 132.00 173.00 187.00 155.00 148.00 154.00 176.00 156.00 159.00 167.00 107.00 185.00 125.00 136.00 158.00 176.00 185.00 161.00 118.00 126.00 151.00 121.00 114.00 181.00 134.00 156.00 172.00 156.00 155.00 152.00 140.00 152.00 136.00 141.00 187.00 165.00 125.00 140.00 144.00 174.00 161.00 133.00 139.00 136.00 124.00 113.00 156.00 133.00 177.00 197.00 99.00 180.00 151.00 154.00 152.00 161.00 169.00 NOT TAKEN 116.00 143.00 186.00 NOT TAKEN 150.00 121.00 133.00 SPILLED 118.00 120.00 183.00 125.00 149.00 60.00 123.00 141.00 136.00 183.00 124.00 148.00 156.00 161.00 147.00 130.00 SPILLED 127.00 131.00 180.00 136.00 24.00 SPILLED 115.00 153.00 157.00 142.00 179.00 175.00 110.00 137.00 119.00 176.00 166.00 138.00 145.00 78.00 161.00 150.00 125.00 126.00 125.00 193.00 116.00 NOT TAKEN 153.00 SPILLED 127.00 119.00 132.00 SPILLED 129.00 112.00 147.00 51.00 135.00 122.00 129.00 151.00 145.00 140.00 94.00 102.00 134.00 75.00 116.00 SPILLED 96.00 209.00 131.00 GROUP: FEMALE 2 - 0.5 PPM C92933 C92934 147.00 133.00 146.00 121.00 171.00 76.00 163.00 NOT TAKEN 1424 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 80 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92935 C92936 C92938 C92940 C92943 C92946 C92947 C92948 C92951 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92963 C92964 C92965 C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92979 C92983 127.00 140.00 169.00 185.00 166.00 142.00 136.00 157.00 125.00 124.00 182.00 152.00 164.00 146.00 143.00 150.00 147.00 186.00 109.00 147.00 147.00 169.00 137.00 143.00 161.00 131.00 162.00 221.00 159.00 121.00 145.00 62.00 135.00 177.00 SPILLED 174.00 126.00 158.00 176.00 158.00 116.00 156.00 145.00 173.00 146.00 141.00 167.00 153.00 SPILLED 117.00 157.00 17.00 171.00 150.00 153.00 170.00 132.00 180.00 213.00 178.00 138.00 135.00 71.00 148.00 171.00 SPILLED 92.00 146.00 146.00 192.00 126.00 119.00 182.00 124.00 159.00 134.00 91.00 175.00 170.00 SPILLED 120.00 170.00 165.00 175.00 181.00 189.00 139.00 181.00 170.00 176.00 132.00 151.00 NOT TAKEN 120.00 173.00 82.00 137.00 111.00 195.00 111.00 124.00 181.00 109.00 167.00 153.00 78.00 184.00 168.00 195.00 132.00 155.00 145.00 189.00 152.00 179.00 147.00 147.00 181.00 176.00 5.00 137.00 111.00 173.00 62.00 146.00 55.00 164.00 132.00 128.00 SPILLED NOT TAKEN 165.00 126.00 189.00 SPILLED 164.00 SPILLED 147.00 143.00 59.00 174.00 138.00 174.00 148.00 169.00 156.00 123.00 200.00 76.00 146.00 19.00 141.00 122.00 174.00 152.00 SPILLED 65.00 SPILLED SPILLED 134.00 132.00 NOT TAKEN 164.00 134.00 146.00 147.00 151.00 144.00 98.00 171.00 157.00 128.00 114.00 7.00 114.00 SPILLED SPILLED 159.00 47.00 174.00 92.00 125.00 36.00 102.00 SPILLED SPILLED 140.00 142.00 SPILLED SPILLED SPILLED 55.00 113.00 93.00 165.00 53.00 1425 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 81 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92984 C92985 C92986 C92988 C92989 C92990 171.00 180.00 122.00 158.00 170.00 158.00 177.00 201.00 125.00 134.00 140.00 148.00 175.00 171.00 147.00 158.00 149.00 136.00 144.00 135.00 146.00 135.00 134.00 118.00 140.00 154.00 142.00 152.00 65.00 131.00 157.00 113.00 146.00 170.00 114.00 146.00 161.00 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92994 C92997 C92999 C93001 C93002 C93003 C93005 C93007 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93022 C93024 C93025 158.00 148.00 126.00 108.00 118.00 128.00 137.00 181.00 137.00 128.00 130.00 158.00 143.00 140.00 72.00 NOT TAKEN 156.00 SPILLED 191.00 168.00 147.00 154.00 164.00 141.00 18.00 85.00 111.00 54.00 36.00 158.00 70.00 124.00 118.00 147.00 161.00 SPILLED 52.00 158.00 107.00 172.00 168.00 63.00 159.00 147.00 NOT TAKEN 151.00 88.00 NOT TAKEN 179.00 127.00 133.00 119.00 121.00 166.00 67.00 170.00 210.00 167.00 98.00 167.00 111.00 SPILLED 140.00 143.00 98.00 48.00 156.00 158.00 130.00 148.00 170.00 155.00 NOT TAKEN SPILLED 164.00 NOT TAKEN 158.00 189.00 145.00 173.00 162.00 144.00 142.00 159.00 92.00 149.00 164.00 146.00 106.00 153.00 168.00 209.00 130.00 142.00 1426 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 82 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93026 C93027 C93028 C93030 C93032 C93034 C93036 C93039 C93040 C93041 C93042 C93043 C93046 C93047 C93048 C93050 167.00 140.00 157.00 157.00 64.00 128.00 197.00 148.00 122.00 148.00 181.00 SPILLED 160.00 214.00 142.00 152.00 132.00 153.00 134.00 145.00 NOT TAKEN 171.00 194.00 141.00 144.00 75.00 178.00 SPILLED NOT TAKEN 204.00 135.00 148.00 132.00 114.00 98.00 157.00 125.00 148.00 135.00 134.00 176.00 200.00 134.00 165.00 149.00 24.00 92.00 135.00 139.00 NOT TAKEN 143.00 109.00 NOT TAKEN 115.00 144.00 142.00 115.00 150.00 200.00 115.00 190.00 144.00 68.00 68.00 155.00 214.00 133.00 171.00 180.00 164.00 SPILLED 213.00 135.00 75.00 154.00 103.00 205.00 149.00 GROUP: FEMALE 4 - 5 PPM C93055 C93056 C93057 C93059 C93060 C93061 C93062 C93063 C93065 C93066 C93068 C93069 7.00 156.00 129.00 161.00 146.00 SPILLED 122.00 152.00 58.00 SPILLED 163.00 161.00 167.00 135.00 152.00 160.00 NOT TAKEN 136.00 135.00 104.00 157.00 141.00 147.00 158.00 143.00 158.00 152.00 132.00 140.00 128.00 138.00 155.00 178.00 155.00 141.00 164.00 167.00 131.00 139.00 126.00 143.00 149.00 202.00 101.00 85.00 167.00 131.00 141.00 150.00 SPILLED 144.00 138.00 212.00 70.00 105.00 157.00 142.00 144.00 154.00 144.00 243.00 162.00 103.00 139.00 153.00 94.00 151.00 158.00 138.00 123.00 124.00 1427 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 83 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93072 C93074 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 136.00 158.00 188.00 169.00 131.00 149.00 154.00 130.00 141.00 152.00 67.00 112.00 126.00 128.00 144.00 111.00 59.00 161.00 132.00 123.00 143.00 128.00 165.00 155.00 137.00 163.00 189.00 3.00 127.00 147.00 101.00 149.00 51.00 160.00 174.00 157.00 168.00 169.00 123.00 144.00 169.00 78.00 133.00 131.00 132.00 148.00 157.00 145.00 130.00 130.00 148.00 162.00 114.00 181.00 197.00 108.00 SPILLED 142.00 128.00 138.00 204.00 182.00 144.00 181.00 SPILLED 125.00 166.00 195.00 118.00 138.00 138.00 146.00 134.00 130.00 164.00 175.00 173.00 162.00 133.00 158.00 170.00 112.00 135.00 140.00 142.00 136.00 160.00 132.00 139.00 138.00 146.00 168.00 126.00 193.00 146.00 NOT TAKEN 56.00 145.00 130.00 147.00 129.00 102.00 137.00 123.00 116.00 156.00 118.00 189.00 170.00 SPILLED 140.00 128.00 145.00 48.00 159.00 181.00 152.00 137.00 132.00 145.00 155.00 150.00 63.00 145.00 142.00 144.00 147.00 150.00 138.00 105.00 136.00 SPILLED 165.00 127.00 96.00 145.00 140.00 157.00 199.00 169.00 SPILLED 138.00 32.00 160.00 126.00 144.00 209.00 184.00 SPILLED SPILLED 139.00 NOT TAKEN 147.00 142.00 NOT TAKEN NOT TAKEN 110.00 114.00 130.00 NOT TAKEN 125.00 SPILLED 59.00 110.00 126.00 130.00 SPILLED 133.00 133.00 154.00 156.00 219.00 160.00 199.00 140.00 161.00 92.00 12.00 125.00 144.00 224.00 138.00 1428 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 84 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93110 192.00 209.00 169.00 83.00 GROUP: FEMALE 5 - 20 PPM C93113 C93115 C93118 C93119 C93121 C93122 C93124 C93125 C93128 C93129 C93131 C93132 C93133 C93135 C93137 C93139 C93140 C93141 C93146 C93148 C93149 C93150 C93151 C93153 C93156 C93157 C93158 129.00 161.00 151.00 141.00 76.00 176.00 128.00 116.00 127.00 155.00 147.00 136.00 140.00 SPILLED 152.00 124.00 176.00 151.00 141.00 158.00 133.00 156.00 131.00 166.00 44.00 143.00 114.00 163.00 155.00 121.00 162.00 219.00 126.00 124.00 148.00 161.00 123.00 129.00 129.00 SPILLED 200.00 141.00 172.00 177.00 141.00 175.00 141.00 153.00 132.00 147.00 196.00 142.00 139.00 140.00 164.00 SPILLED 155.00 223.00 123.00 127.00 149.00 168.00 163.00 140.00 134.00 SPILLED 178.00 136.00 178.00 175.00 144.00 178.00 139.00 136.00 127.00 166.00 141.00 151.00 149.00 157.00 157.00 194.00 159.00 152.00 160.00 111.00 150.00 143.00 152.00 144.00 126.00 SPILLED 146.00 SPILLED 178.00 162.00 147.00 173.00 154.00 142.00 122.00 163.00 148.00 146.00 165.00 163.00 168.00 NOT TAKEN NOT TAKEN 138.00 116.00 146.00 97.00 78.00 116.00 123.00 SPILLED 161.00 118.00 182.00 167.00 132.00 130.00 NOT TAKEN 137.00 139.00 144.00 140.00 131.00 167.00 144.00 112.00 143.00 65.00 NOT TAKEN 131.00 136.00 SPILLED 104.00 128.00 174.00 133.00 126.00 155.00 122.00 204.00 157.00 145.00 159.00 129.00 177.00 SPILLED 201.00 134.00 SPILLED 145.00 121.00 164.00 NOT TAKEN 137.00 58.00 149.00 135.00 150.00 150.00 171.00 118.00 176.00 SPILLED 2.00 134.00 92.00 119.00 135.00 127.00 106.00 94.00 142.00 SPILLED 155.00 145.00 1429 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 85 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93160 C93161 C93162 C93164 C93165 C93166 C93168 C93170 C93172 C93173 C93174 C93175 C93176 C93177 C93178 C93179 NOT TAKEN 184.00 141.00 159.00 138.00 139.00 136.00 SPILLED 73.00 139.00 153.00 119.00 130.00 133.00 114.00 112.00 135.00 137.00 147.00 139.00 125.00 137.00 196.00 125.00 164.00 106.00 110.00 153.00 111.00 119.00 193.00 142.00 160.00 138.00 155.00 146.00 165.00 109.00 SPILLED 106.00 76.00 129.00 120.00 108.00 SPILLED 139.00 156.00 118.00 126.00 178.00 115.00 164.00 143.00 113.00 NOT TAKEN 112.00 117.00 111.00 202.00 140.00 175.00 137.00 142.00 132.00 101.00 153.00 147.00 NOT TAKEN 109.00 114.00 117.00 209.00 133.00 189.00 153.00 139.00 131.00 139.00 SPILLED 124.00 119.00 123.00 215.00 124.00 147.00 133.00 150.00 122.00 97.00 150.00 133.00 104.00 95.00 221.00 SPILLED 164.00 127.00 139.00 130.00 150.00 148.00 120.00 139.00 GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93187 C93188 C93189 C93190 C93191 C93192 C93193 130.00 136.00 146.00 153.00 149.00 144.00 178.00 127.00 158.00 179.00 171.00 NOT TAKEN 128.00 159.00 164.00 157.00 148.00 172.00 176.00 130.00 154.00 186.00 143.00 137.00 159.00 163.00 156.00 148.00 161.00 170.00 64.00 163.00 196.00 143.00 117.00 143.00 168.00 158.00 141.00 59.00 188.00 143.00 SPILLED 167.00 144.00 150.00 173.00 175.00 160.00 SPILLED 186.00 134.00 149.00 143.00 159.00 167.00 161.00 158.00 133.00 SPILLED 59.00 159.00 133.00 152.00 141.00 171.00 155.00 84.00 SPILLED 67.00 168.00 150.00 152.00 SPILLED 163.00 150.00 NOT TAKEN 116.00 154.00 132.00 1430 Appendix 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) PAGE: 86 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93194 C93196 C93197 C93198 C93200 C93201 C93202 C93203 C93204 C93205 C93206 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 149.00 132.00 146.00 144.00 129.00 141.00 130.00 139.00 139.00 158.00 161.00 159.00 204.00 150.00 129.00 154.00 115.00 157.00 123.00 163.00 176.00 151.00 177.00 158.00 149.00 128.00 159.00 171.00 128.00 130.00 109.00 159.00 129.00 146.00 155.00 142.00 151.00 151.00 144.00 101.00 99.00 175.00 59.00 143.00 142.00 141.00 187.00 159.00 135.00 105.00 152.00 164.00 150.00 129.00 133.00 84.00 146.00 152.00 154.00 187.00 181.00 153.00 NOT TAKEN NOT TAKEN 167.00 SPILLED 162.00 196.00 139.00 185.00 165.00 189.00 NOT TAKEN SPILLED 137.00 149.00 126.00 134.00 130.00 SPILLED 141.00 158.00 171.00 173.00 131.00 167.00 158.00 167.00 128.00 191.00 151.00 NOT TAKEN SPILLED 158.00 120.00 156.00 132.00 NOT TAKEN 140.00 151.00 169.00 137.00 148.00 184.00 168.00 183.00 163.00 151.00 155.00 NOT TAKEN 122.00 147.00 162.00 29.00 SPILLED SPILLED 131.00 SPILLED 163.00 194.00 SPILLED 144.00 146.00 143.00 140.00 146.00 37.00 170.00 54.00 143.00 156.00 145.00 156.00 159.00 93.00 NOT TAKEN 172.00 30.00 73.00 164.00 192.00 150.00 171.00 206.00 154.00 1431 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 0.055 0.061 0.056 0.053 0.053 0.051 0.060 0.060 0.052 0.059 0.054 0.061 0.054 SPILLED 0.054 0.057 0.060 SPILLED 0.058 0.061 0.052 0.060 0.053 0.056 0.053 0.053 0.053 0.059 0.051 0.058 0.059 0.049 0.049 0.049 0.046 CNTMD 0.044 0.050 0.047 0.045 0.049 0.044 0.050 SPILLED 0.050 CNTMD 0.046 0.047 0.045 0.048 0.050 0.043 0.049 0.045 0.047 0.045 0.044 0.044 0.048 0.044 0.046 0.049 0.044 0.042 SPILLED SPILLED 0.038 0.039 0.044 0.040 0.041 0.042 0.039 SPILLED 0.039 0.045 0.040 0.042 0.042 0.041 0.043 0.043 0.040 0.042 0.039 SPILLED 0.044 0.041 0.040 0.045 0.039 0.043 0.045 0.038 0.041 0.036 NOT TAKEN NOT TAKEN 0.036 0.039 0.037 0.037 0.038 0.036 SPILLED SPILLED 0.041 0.032 0.037 0.037 0.037 0.038 0.039 0.037 0.038 0.036 0.040 0.036 0.038 0.037 0.040 0.034 0.039 0.040 0.035 0.037 0.037 NOT TAKEN NOT TAKEN 0.033 0.035 0.035 0.035 0.036 0.032 0.034 SPILLED 0.037 0.035 0.038 0.038 0.037 0.036 0.035 0.034 0.034 0.032 0.037 0.034 0.036 0.039 0.036 0.035 0.036 0.034 0.035 0.035 0.035 NOT TAKEN NOT TAKEN 0.031 SPILLED SPILLED 0.032 SPILLED 0.031 0.032 SPILLED 0.035 0.033 0.034 0.034 0.034 0.033 SPILLED 0.031 0.032 0.030 0.036 0.033 0.034 0.036 0.035 SPILLED 0.034 0.032 SPILLED 0.034 SPILLED NOT TAKEN NOT TAKEN 0.029 0.035 0.035 0.032 SPILLED 0.031 0.033 SPILLED 0.033 CNTMD 0.033 0.034 SPILLED 0.032 0.026 SPILLED 0.031 0.029 0.035 0.031 0.032 0.034 0.033 SPILLED 0.033 0.030 0.033 0.031 0.030 NOT TAKEN NOT TAKEN 0.029 0.030 0.030 0.030 0.029 0.029 0.030 SPILLED 0.032 0.031 0.031 0.030 SPILLED 0.029 0.030 SPILLED 0.029 0.027 0.033 0.030 0.031 0.032 0.031 0.030 0.030 0.028 SPILLED 0.031 0.029 NOT TAKEN NOT TAKEN 0.027 0.027 0.029 0.027 0.028 0.027 0.029 0.028 0.031 0.027 0.029 0.028 SPILLED 0.028 0.029 0.027 0.027 0.026 SPILLED 0.028 0.030 SPILLED 0.029 0.029 0.029 0.027 0.032 0.031 0.026 NOT TAKEN NOT TAKEN 0.026 0.027 0.029 0.026 SPILLED 0.025 0.026 0.028 0.029 0.028 0.028 0.027 SPILLED 0.027 0.028 0.026 0.026 0.026 0.030 0.027 0.029 0.030 0.028 0.027 0.027 0.025 1432 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 0.059 0.055 0.052 0.051 0.057 0.063 0.055 0.057 0.054 0.054 0.057 0.062 0.055 0.065 0.060 0.058 0.062 0.057 0.056 0.055 CNTMD 0.054 CNTMD 0.061 0.061 0.056 0.053 0.064 0.059 0.047 SPILLED 0.046 0.042 0.048 0.052 0.045 0.047 0.045 0.045 0.046 0.052 0.047 0.050 0.048 0.048 0.055 SPILLED 0.050 0.046 0.050 0.046 0.039 SPILLED 0.048 0.047 0.045 0.052 0.047 0.043 SPILLED 0.041 0.039 0.046 0.046 0.043 0.042 SPILLED 0.040 0.042 0.044 0.042 0.046 0.044 0.043 SPILLED 0.046 0.046 0.042 0.042 0.040 0.038 SPILLED 0.042 0.044 0.041 0.046 0.042 0.042 SPILLED 0.037 0.035 0.039 0.041 0.038 0.038 NOT TAKEN 0.037 0.036 NOT TAKEN SPILLED 0.035 SPILLED 0.039 NOT TAKEN 0.040 SPILLED 0.039 0.039 0.039 0.035 SPILLED 0.039 SPILLED 0.042 0.045 0.037 0.037 0.034 0.034 0.034 0.036 0.037 SPILLED 0.035 NOT TAKEN 0.034 0.033 NOT TAKEN 0.039 0.036 SPILLED 0.036 NOT TAKEN 0.036 0.036 0.039 0.035 0.036 0.034 SPILLED 0.034 0.041 SPILLED 0.040 0.035 0.034 SPILLED 0.033 SPILLED 0.034 0.034 SPILLED 0.033 NOT TAKEN 0.032 0.030 NOT TAKEN 0.037 0.034 0.034 0.033 NOT TAKEN 0.033 0.034 0.035 0.033 0.034 0.031 SPILLED 0.031 SPILLED 0.036 SPILLED 0.033 0.033 SPILLED 0.032 0.030 0.032 SPILLED SPILLED 0.032 NOT TAKEN 0.030 0.029 NOT TAKEN 0.036 0.032 0.033 0.032 NOT TAKEN 0.033 0.032 0.033 0.030 0.032 0.031 SPILLED 0.032 SPILLED 0.033 0.034 0.035 SPILLED SPILLED 0.030 0.028 0.032 SPILLED SPILLED 0.029 NOT TAKEN 0.028 0.029 NOT TAKEN SPILLED 0.031 0.032 0.029 NOT TAKEN 0.031 0.032 0.033 0.029 0.028 0.029 SPILLED 0.031 SPILLED 0.032 0.033 0.036 0.032 0.029 0.029 0.028 0.031 0.030 0.030 0.028 NOT TAKEN 0.027 0.027 NOT TAKEN SPILLED 0.029 SPILLED 0.028 NOT TAKEN 0.030 0.029 0.031 0.028 0.028 0.029 0.029 0.029 SPILLED SPILLED 0.031 0.032 0.029 0.028 0.027 0.026 0.029 0.028 0.029 0.026 NOT TAKEN 0.026 0.026 NOT TAKEN SPILLED 0.027 0.031 0.025 NOT TAKEN 0.028 0.025 0.029 0.026 0.028 0.028 SPILLED 0.028 SPILLED SPILLED SPILLED 0.031 1433 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 0.223 0.215 0.237 0.246 0.259 0.248 0.202 0.229 0.222 0.207 0.214 0.211 0.246 0.227 0.233 0.222 0.200 0.219 0.222 0.245 0.221 0.246 0.224 0.240 0.254 0.227 0.229 0.209 0.219 0.219 0.202 0.188 0.174 0.195 0.207 0.214 SPILLED 0.174 0.193 0.186 0.183 0.193 0.180 0.196 0.181 0.186 0.181 0.179 0.190 0.195 0.207 0.189 0.207 0.187 0.207 0.212 0.185 0.207 0.178 0.188 0.172 0.171 0.164 0.154 SPILLED 0.183 0.183 0.173 0.160 0.180 0.166 0.160 0.166 0.165 0.171 0.157 SPILLED 0.156 0.160 0.171 0.173 0.176 0.166 0.181 0.163 0.187 0.190 0.162 0.169 0.167 0.165 0.174 0.155 0.150 0.129 0.145 SPILLED SPILLED SPILLED 0.152 0.165 0.149 0.147 0.159 0.153 0.151 0.142 0.155 NOT TAKEN 0.142 0.151 0.160 NOT TAKEN 0.148 NOT TAKEN 0.148 0.163 0.169 0.151 0.144 0.170 0.156 0.177 0.139 0.138 0.133 0.147 0.151 0.146 SPILLED 0.132 0.151 0.143 0.132 0.140 0.140 0.135 0.129 0.141 NOT TAKEN 0.132 0.136 0.148 NOT TAKEN 0.137 NOT TAKEN 0.131 SPILLED 0.162 0.135 SPILLED 0.136 0.139 0.132 0.126 0.131 0.124 0.140 0.139 0.142 0.143 0.126 0.141 0.137 0.127 0.131 0.135 0.128 0.125 0.135 NOT TAKEN 0.127 0.131 0.143 NOT TAKEN 0.123 NOT TAKEN 0.122 SPILLED 0.151 0.123 SPILLED SPILLED 0.134 0.138 0.119 0.124 0.117 0.131 0.131 SPILLED 0.143 0.118 0.134 SPILLED 0.128 0.119 0.121 0.119 0.113 0.123 NOT TAKEN 0.120 0.119 0.136 NOT TAKEN 0.119 NOT TAKEN 0.116 SPILLED SPILLED 0.119 0.130 0.123 0.130 0.121 0.113 0.124 0.113 0.124 0.126 0.122 0.148 0.119 0.128 0.123 0.127 0.118 0.115 0.116 0.114 0.121 NOT TAKEN 0.117 0.119 0.126 NOT TAKEN 0.115 NOT TAKEN 0.115 0.127 SPILLED 0.112 SPILLED 0.121 0.122 0.125 0.114 0.110 0.102 0.117 0.116 0.116 SPILLED 0.106 0.119 0.108 0.116 0.112 0.109 0.113 0.112 0.116 NOT TAKEN 0.109 0.111 0.120 NOT TAKEN 0.112 NOT TAKEN 0.112 0.116 SPILLED 0.104 0.116 0.109 0.117 0.113 0.098 0.110 0.102 0.113 0.120 SPILLED SPILLED 0.108 0.115 0.108 0.120 0.111 0.107 0.111 0.114 0.120 NOT TAKEN 0.107 0.116 0.124 NOT TAKEN 0.108 NOT TAKEN 0.105 SPILLED SPILLED 0.105 0.116 0.114 0.119 SPILLED 0.103 1434 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 0.225 0.255 0.208 0.238 0.221 0.225 0.214 0.226 0.239 0.254 0.227 0.218 0.242 0.232 0.219 0.213 0.244 0.230 0.202 0.227 0.217 0.231 0.222 0.228 0.208 0.207 0.219 0.239 0.232 0.196 0.219 0.187 0.194 0.185 0.195 0.185 0.192 0.197 0.209 0.185 0.171 0.193 0.190 0.178 0.179 0.206 0.189 0.185 SPILLED 0.187 0.185 0.178 0.183 0.171 0.168 0.181 0.194 0.194 0.191 0.191 0.162 0.173 0.169 0.182 0.168 0.177 0.171 0.187 0.164 0.152 0.165 0.168 SPILLED 0.155 0.177 0.164 0.165 0.163 0.163 0.170 0.161 0.169 0.157 0.157 0.167 0.169 SPILLED SPILLED 0.175 SPILLED 0.156 0.153 0.162 NOT TAKEN 0.161 0.152 0.163 0.146 0.136 0.160 0.147 0.129 0.137 NOT TAKEN 0.146 0.145 SPILLED 0.137 0.146 0.141 0.151 0.130 0.147 0.156 0.146 0.151 0.144 SPILLED 0.139 0.142 0.141 0.147 NOT TAKEN 0.146 0.142 0.147 0.129 0.131 0.138 0.135 0.147 0.128 NOT TAKEN 0.149 0.140 0.136 0.134 0.138 0.143 0.140 0.133 0.143 SPILLED 0.136 0.131 0.133 SPILLED 0.132 0.134 0.130 0.139 NOT TAKEN 0.139 0.134 0.140 0.134 0.121 SPILLED 0.128 0.128 SPILLED NOT TAKEN 0.128 SPILLED SPILLED 0.126 0.132 0.132 0.127 0.126 0.133 SPILLED 0.131 0.117 0.124 SPILLED 0.132 0.125 0.127 0.133 NOT TAKEN 0.133 0.126 0.108 0.128 0.118 SPILLED 0.123 0.123 0.103 NOT TAKEN 0.121 0.116 SPILLED 0.118 0.121 0.123 0.124 0.120 0.124 SPILLED 0.120 0.071 0.123 SPILLED 0.120 0.123 0.123 0.130 NOT TAKEN 0.134 0.122 0.131 0.123 0.111 SPILLED 0.118 0.119 0.110 NOT TAKEN 0.122 SPILLED SPILLED 0.119 SPILLED 0.123 0.121 0.113 0.121 SPILLED 0.111 0.117 0.114 0.117 0.114 0.117 0.118 0.118 NOT TAKEN 0.128 0.111 0.121 0.114 0.107 0.131 0.118 0.109 0.109 NOT TAKEN 0.116 0.116 SPILLED 0.109 0.110 0.115 0.113 0.107 0.114 SPILLED 0.101 0.108 0.115 SPILLED 0.111 0.112 0.118 0.116 NOT TAKEN 0.125 0.117 0.122 0.118 SPILLED 0.133 0.116 0.107 0.108 NOT TAKEN 0.114 0.116 SPILLED 0.112 0.110 0.115 0.113 0.107 0.112 SPILLED 0.103 0.106 1435 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 0.610 0.613 0.622 0.526 0.563 0.510 0.561 0.582 0.562 0.553 0.500 0.617 0.609 0.543 0.553 0.576 0.512 0.591 0.554 0.596 0.536 0.579 0.572 0.611 0.542 0.533 0.630 0.637 0.656 0.654 0.545 0.477 0.472 SPILLED 0.409 0.450 0.427 0.467 0.448 0.458 0.471 0.421 0.491 0.513 0.455 0.445 0.474 0.446 0.477 0.466 0.502 0.433 0.465 SPILLED SPILLED SPILLED 0.439 0.496 0.526 0.525 SPILLED 0.476 0.421 0.433 0.437 0.368 0.393 0.387 0.426 0.416 0.417 0.406 0.389 0.424 0.463 0.409 0.414 0.404 0.385 0.412 0.402 0.435 0.399 0.396 SPILLED 0.449 0.399 0.393 0.434 0.478 0.439 SPILLED 0.416 0.371 0.360 0.400 0.332 SPILLED SPILLED 0.371 0.368 0.371 0.371 0.331 0.374 0.410 0.369 0.380 0.359 0.333 0.344 0.347 0.390 0.344 0.350 SPILLED 0.363 NOT TAKEN 0.322 0.366 NOT TAKEN 0.382 SPILLED 0.319 0.348 0.337 0.377 SPILLED 0.341 SPILLED SPILLED 0.346 0.340 0.330 0.316 0.349 0.371 0.344 0.359 0.327 0.309 0.299 0.361 SPILLED 0.336 0.343 0.292 0.351 NOT TAKEN 0.335 0.333 NOT TAKEN 0.356 SPILLED 0.328 0.344 0.333 0.349 0.303 SPILLED SPILLED 0.340 0.312 0.330 0.313 0.292 0.312 0.355 0.322 0.325 0.306 0.281 0.292 0.334 0.341 0.308 0.323 SPILLED 0.327 NOT TAKEN 0.317 0.323 NOT TAKEN 0.334 SPILLED 0.334 0.328 0.303 0.323 0.294 0.308 0.321 0.320 SPILLED 0.285 0.296 0.286 0.301 0.334 0.309 0.328 0.283 0.273 0.294 0.325 SPILLED 0.303 SPILLED SPILLED SPILLED NOT TAKEN 0.303 0.313 NOT TAKEN 0.317 SPILLED 0.321 0.295 0.285 0.297 0.278 SPILLED SPILLED SPILLED 0.290 0.293 0.304 0.268 0.280 0.318 0.294 0.307 0.273 0.266 0.289 0.294 SPILLED 0.276 0.286 SPILLED SPILLED NOT TAKEN 0.279 0.294 NOT TAKEN 0.300 SPILLED 0.307 0.293 0.273 0.289 0.270 0.260 SPILLED 0.319 0.281 0.283 0.289 0.267 0.279 0.304 0.287 0.307 0.270 0.271 0.289 0.278 SPILLED 0.266 SPILLED SPILLED 0.306 NOT TAKEN 0.268 0.293 NOT TAKEN SPILLED SPILLED 0.304 0.278 0.263 0.282 0.255 0.261 SPILLED 0.286 0.268 0.286 0.275 0.240 0.258 0.292 0.264 0.298 0.249 0.242 0.271 0.261 0.283 0.249 0.274 0.265 0.287 NOT TAKEN 0.253 0.264 NOT TAKEN 0.289 SPILLED 0.267 1436 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 0.562 0.571 0.611 0.591 0.576 0.520 0.599 0.523 0.605 SPILLED 0.645 0.562 0.580 0.504 0.555 0.510 0.590 0.543 0.601 0.495 0.594 0.612 0.595 0.540 0.541 0.540 0.514 0.590 0.546 0.463 0.513 0.517 0.495 0.487 0.457 0.484 0.459 0.503 SPILLED 0.539 0.464 0.479 0.433 0.459 0.431 0.496 0.446 0.490 0.421 0.491 0.510 0.533 0.470 0.475 0.450 0.425 0.471 0.468 0.410 SPILLED 0.438 0.422 0.432 0.413 0.454 0.428 0.461 0.383 0.484 0.419 0.420 0.381 0.420 0.402 0.462 0.412 0.422 0.373 0.432 0.434 0.472 0.426 0.405 0.414 0.388 0.421 0.441 0.365 SPILLED 0.392 0.343 0.373 SPILLED 0.383 0.369 SPILLED NOT TAKEN 0.379 0.356 NOT TAKEN 0.349 0.362 0.345 0.381 0.374 0.370 0.351 0.370 0.390 NOT TAKEN 0.384 0.335 0.378 0.351 0.373 0.380 0.351 SPILLED 0.368 0.368 0.353 0.357 0.353 0.348 0.373 NOT TAKEN 0.389 0.337 NOT TAKEN 0.333 0.345 0.333 0.370 0.359 0.342 0.321 0.344 0.362 NOT TAKEN 0.362 0.342 0.365 0.342 0.352 0.353 0.315 SPILLED 0.354 0.327 0.322 0.335 0.336 0.312 0.348 NOT TAKEN 0.345 0.309 NOT TAKEN 0.312 0.324 0.305 0.339 0.333 0.321 0.303 0.316 0.329 NOT TAKEN 0.340 SPILLED 0.349 0.331 0.343 0.322 0.305 SPILLED SPILLED 0.302 0.310 0.316 0.312 0.296 0.331 NOT TAKEN 0.333 0.293 NOT TAKEN 0.302 0.306 0.290 0.330 0.324 0.323 0.317 0.296 0.315 NOT TAKEN 0.321 SPILLED 0.327 0.349 0.325 SPILLED 0.294 0.305 0.302 0.288 0.296 SPILLED 0.286 0.281 0.296 NOT TAKEN 0.311 0.294 NOT TAKEN 0.282 0.290 0.277 0.301 0.307 0.300 SPILLED 0.285 SPILLED NOT TAKEN 0.299 SPILLED 0.317 SPILLED 0.300 0.301 0.297 SPILLED 0.303 SPILLED 0.279 0.288 0.268 0.283 0.288 NOT TAKEN 0.311 0.306 NOT TAKEN 0.280 0.298 0.287 0.303 0.326 0.289 0.268 0.274 0.287 NOT TAKEN 0.289 0.269 SPILLED 0.318 0.300 0.274 0.215 SPILLED SPILLED 0.280 0.266 0.277 0.276 0.267 0.284 NOT TAKEN 0.298 0.290 NOT TAKEN 0.263 0.277 0.267 0.291 0.304 0.260 0.257 0.261 0.276 NOT TAKEN 0.269 0.251 SPILLED SPILLED SPILLED 0.267 1437 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 2.238 2.125 2.223 2.024 2.444 2.144 2.295 2.215 1.973 2.117 2.109 2.172 2.283 2.355 2.097 2.309 2.195 1.996 2.288 2.023 2.368 2.350 2.024 2.332 2.590 2.198 2.456 1.921 2.228 2.255 2.156 1.990 1.717 1.889 1.807 1.960 1.888 1.921 1.876 1.673 1.853 1.912 1.793 1.839 2.001 1.816 1.920 1.923 1.781 1.883 SPILLED 1.886 1.916 1.768 1.927 2.139 1.844 2.004 1.775 1.865 2.058 1.836 1.824 1.618 1.660 1.686 1.831 1.828 1.809 1.657 1.535 1.687 1.625 1.662 1.683 1.790 1.642 1.657 1.764 1.590 1.797 1.523 1.823 1.774 1.588 1.653 1.895 1.592 1.730 1.606 1.684 1.671 1.617 1.586 NOT TAKEN 1.526 1.492 1.514 1.638 1.688 1.424 NOT TAKEN 1.488 1.510 1.522 1.440 1.497 1.467 1.458 1.520 1.403 1.491 1.319 1.709 1.439 1.424 1.490 1.599 1.413 1.509 1.412 NOT TAKEN 1.550 1.441 1.429 NOT TAKEN 1.427 1.391 1.448 1.473 1.537 1.311 NOT TAKEN 1.369 1.357 1.384 1.377 1.509 1.372 1.373 1.345 1.330 1.401 1.283 SPILLED 1.361 1.322 1.454 1.522 1.333 1.414 1.230 NOT TAKEN 1.451 1.316 1.295 NOT TAKEN 1.248 1.291 1.298 1.425 1.440 1.259 NOT TAKEN 1.286 1.289 1.312 1.234 1.411 1.274 1.269 1.354 1.266 1.297 1.152 1.305 1.281 1.179 1.363 1.378 1.264 1.288 1.195 NOT TAKEN 1.363 1.263 SPILLED NOT TAKEN 1.282 1.327 1.268 SPILLED SPILLED 1.178 NOT TAKEN 1.274 1.194 1.199 1.232 1.345 1.276 1.222 1.273 1.157 1.273 1.163 SPILLED 1.163 1.147 1.291 1.338 1.205 1.301 1.184 NOT TAKEN 1.346 1.212 SPILLED NOT TAKEN 1.187 1.160 1.171 SPILLED SPILLED 1.088 NOT TAKEN 1.150 1.186 SPILLED 1.155 1.316 1.158 1.167 1.291 1.015 1.159 1.065 1.093 1.116 1.074 1.201 1.250 1.140 1.173 1.117 NOT TAKEN 1.276 0.921 1.086 NOT TAKEN 1.166 1.129 1.196 SPILLED 1.217 1.033 NOT TAKEN 1.112 1.114 1.068 1.115 SPILLED 1.117 1.148 1.251 1.033 1.108 1.016 1.061 1.118 1.093 1.203 1.141 1.092 1.151 1.077 NOT TAKEN 1.229 1.081 1.044 NOT TAKEN 0.982 1.121 1.112 SPILLED 1.160 0.983 NOT TAKEN 1.105 1.093 1.066 1.061 1.151 1.085 1.115 1.196 0.970 1.076 0.997 1.004 1.049 0.987 1.098 1.150 1.071 1.144 1.067 NOT TAKEN SPILLED 1.048 1438 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 2.583 2.109 2.165 2.051 2.136 2.134 2.438 2.233 2.514 2.101 2.078 2.122 2.035 2.163 2.002 1.892 2.390 2.150 2.221 1.993 2.221 2.051 2.265 2.170 2.348 2.336 2.354 2.179 2.169 2.391 2.072 2.064 1.739 1.768 1.804 1.741 1.821 2.089 1.824 1.944 1.745 1.937 1.703 1.694 1.823 1.690 1.612 1.820 1.711 1.898 1.693 1.797 1.720 1.803 1.687 1.897 1.978 1.896 1.924 1.803 1.972 1.715 1.887 1.633 1.718 1.675 1.618 1.749 1.833 1.709 1.750 1.717 1.613 1.524 1.642 1.657 1.548 1.481 1.652 1.612 1.696 1.604 1.594 1.569 1.698 1.562 1.670 1.762 1.782 1.654 1.657 1.792 1.523 1.617 1.425 1.373 1.506 1.401 1.584 SPILLED 1.498 1.506 1.531 1.403 NOT TAKEN 1.371 1.480 1.433 1.361 NOT TAKEN 1.382 SPILLED 1.470 1.437 1.458 1.494 1.300 1.453 1.435 1.512 1.469 1.465 1.465 1.340 1.509 1.350 1.461 1.417 1.338 1.420 1.555 1.382 1.390 1.353 1.362 NOT TAKEN 1.308 1.363 1.340 1.265 NOT TAKEN 1.402 1.392 1.375 1.352 1.379 1.463 1.393 1.348 1.561 1.397 1.453 1.412 1.563 1.293 1.381 1.270 1.335 1.361 1.236 1.334 1.304 1.300 1.304 1.300 1.271 NOT TAKEN 1.261 1.285 1.273 1.215 NOT TAKEN 1.318 1.280 1.220 1.279 1.226 1.289 1.234 1.201 1.354 1.238 1.264 1.278 1.411 1.257 1.376 1.214 1.190 1.287 1.226 1.338 1.263 1.272 1.254 1.296 1.242 NOT TAKEN 1.237 1.252 1.155 1.180 NOT TAKEN 1.184 SPILLED 1.156 1.228 1.162 1.222 1.209 1.170 1.298 1.225 1.249 1.241 1.350 1.186 1.163 1.124 1.196 SPILLED 1.173 0.886 1.022 1.108 1.148 1.217 1.174 NOT TAKEN 1.174 1.212 1.101 1.141 NOT TAKEN 1.088 1.173 1.097 1.175 1.119 1.217 1.100 0.946 1.223 1.098 1.198 1.232 1.277 1.302 1.149 1.023 1.149 1.154 1.055 SPILLED 1.159 1.091 1.089 1.096 1.089 NOT TAKEN 1.101 1.148 1.024 1.074 NOT TAKEN 1.068 SPILLED SPILLED 1.086 1.075 1.164 1.084 0.801 1.111 1.043 1.170 1.130 1.200 0.995 1.105 1.018 1.127 1.129 1.076 SPILLED 1.090 SPILLED 1.058 1.101 1.056 NOT TAKEN 1.049 1.107 0.996 1.063 NOT TAKEN 1.058 SPILLED 0.987 1.084 1.019 1.112 1.054 0.971 1.037 1.056 1.074 1.088 1.180 1.011 1439 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 2.323 2.439 2.006 2.200 2.154 2.041 2.404 SPILLED 1.870 1.983 1.600 1.816 1.814 1.663 1.939 1.912 1.649 1.787 1.514 1.637 1.652 1.499 1.798 1.637 1.493 1.534 SPILLED 1.431 1.459 1.386 1.570 1.542 1.366 1.388 1.414 1.347 1.351 1.307 1.429 1.501 1.257 1.292 1.102 1.219 1.241 1.224 1.355 1.432 1.302 1.189 1.313 1.172 1.207 1.148 1.265 1.333 1.214 1.162 1.434 1.127 1.223 1.217 1.269 1.274 1.127 1.047 1.305 0.993 1.063 1.091 1.122 1.266 1.102 1.047 1.290 1.024 1.059 1.070 1.136 SPILLED GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 2.391 2.367 2.310 2.097 2.453 2.215 2.096 2.214 2.323 2.562 2.323 2.396 2.411 2.232 2.409 2.079 2.353 2.455 2.592 2.134 1.883 1.905 1.865 1.695 1.997 SPILLED 1.769 1.786 1.819 2.071 1.944 1.988 1.901 1.901 1.793 1.752 1.948 1.946 2.137 1.710 1.730 1.641 1.698 1.526 1.754 1.717 1.650 1.638 1.710 1.808 1.720 1.706 1.705 1.726 1.533 1.586 1.728 1.721 SPILLED 1.512 1.480 1.480 1.459 1.421 1.568 1.510 1.507 1.433 1.525 1.731 1.471 1.499 1.474 1.507 1.426 1.438 1.548 1.568 1.681 1.439 1.397 1.402 1.369 1.336 1.502 1.382 1.448 1.385 1.475 1.579 1.360 1.400 1.358 1.368 1.309 1.352 1.401 1.438 1.553 1.295 1.312 1.361 1.222 1.274 1.404 1.289 1.340 1.286 1.385 1.431 1.268 1.266 1.205 1.279 1.290 1.233 1.258 1.322 1.368 1.219 1.211 1.249 1.150 1.218 1.302 SPILLED 1.293 1.217 1.291 1.271 1.245 SPILLED SPILLED 1.213 1.168 1.166 1.186 1.258 1.254 1.133 1.136 1.166 1.096 1.120 1.255 SPILLED 1.266 1.171 1.242 1.339 1.232 SPILLED SPILLED 1.145 1.123 0.731 1.099 1.217 SPILLED 1.065 1.071 1.114 1.081 1.162 1.178 1.221 1.154 1.137 1.175 1.296 1.139 SPILLED SPILLED 1.143 1.109 1.091 1.043 1.177 1.173 1.079 1.073 1.067 1.070 1.195 SPILLED 1.233 1.174 1.095 1.162 1.299 1.146 1.021 1.035 1.093 1.065 1.259 1.033 1.118 1.140 1.052 1440 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 2.011 2.260 2.248 2.442 SPILLED 2.491 2.519 2.611 2.521 2.122 2.213 2.393 2.159 2.416 2.489 2.719 2.228 2.214 2.121 2.519 1.822 1.830 1.892 1.892 SPILLED 1.935 2.155 2.062 2.072 1.800 1.890 1.994 1.819 2.022 SPILLED 2.028 1.864 1.762 1.848 2.070 1.575 1.616 1.726 1.685 1.728 1.680 1.890 1.873 1.763 1.639 1.607 1.861 1.602 1.811 SPILLED 1.786 1.620 1.766 1.711 1.905 1.515 1.452 1.506 1.519 1.610 1.543 1.624 1.709 1.551 1.513 1.476 1.782 1.483 SPILLED SPILLED 1.527 1.486 1.531 1.523 1.652 1.267 1.276 1.400 1.377 1.515 1.406 1.434 1.552 1.473 1.394 1.341 1.451 1.359 1.425 1.402 1.425 1.322 1.460 1.481 1.468 1.225 1.182 1.281 1.293 1.419 1.354 1.326 1.460 1.366 SPILLED 1.223 1.297 1.287 1.297 SPILLED 1.302 1.263 1.281 1.378 1.407 1.141 1.160 1.190 1.264 1.331 1.288 1.243 1.361 1.322 1.338 1.190 1.323 1.198 1.246 0.943 1.213 1.176 1.251 1.316 1.309 1.114 1.096 1.133 1.204 1.248 1.219 1.219 1.312 SPILLED 1.265 1.134 1.347 1.208 1.211 SPILLED 1.163 1.157 1.172 1.201 1.237 1.068 1.062 1.123 1.163 SPILLED 1.140 1.137 1.272 SPILLED SPILLED 1.067 SPILLED 1.120 1.106 SPILLED 1.124 1.036 1.112 SPILLED 1.255 1.044 1.039 1.088 1.108 1.236 1.149 1.134 1.260 1.251 1.231 1.050 1.252 1.057 1.134 1.200 1.106 1.022 1.081 SPILLED 1.175 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 0.052 SPILLED 0.051 0.053 0.054 0.053 0.050 0.050 0.049 SPILLED 0.050 0.052 0.050 0.049 0.050 SPILLED 0.046 SPILLED 0.046 0.050 0.051 0.048 0.046 SPILLED 0.042 SPILLED 0.047 SPILLED 0.046 0.040 0.043 SPILLED 0.040 SPILLED 0.043 0.043 0.042 0.042 0.042 SPILLED 0.038 SPILLED SPILLED SPILLED 0.036 0.038 0.040 SPILLED 0.036 SPILLED 0.040 0.046 0.036 0.036 0.040 SPILLED 0.036 SPILLED 0.039 0.038 0.036 0.035 SPILLED SPILLED 0.033 SPILLED 0.039 0.036 0.034 0.034 0.037 SPILLED 0.031 SPILLED 0.038 SPILLED 0.032 0.030 SPILLED SPILLED 1441 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 0.047 0.053 0.057 0.053 0.054 0.050 0.054 0.047 0.052 0.048 SPILLED 0.054 0.048 0.048 0.050 0.054 0.055 0.049 SPILLED 0.047 0.054 SPILLED SPILLED 0.048 0.046 0.049 0.059 0.049 0.056 0.056 0.048 0.046 0.051 SPILLED SPILLED 0.049 SPILLED 0.056 0.047 0.049 0.052 SPILLED 0.048 0.047 0.046 0.046 0.053 0.049 0.046 0.050 0.050 0.052 0.061 SPILLED 0.048 0.045 0.067 0.064 0.045 0.052 SPILLED 0.051 0.056 0.051 SPILLED SPILLED 0.049 0.045 0.051 0.044 0.048 0.045 0.054 0.050 SPILLED 0.047 SPILLED 0.046 0.049 0.047 0.048 0.043 0.049 SPILLED SPILLED 0.046 0.043 0.048 SPILLED 0.041 0.050 0.050 SPILLED 0.043 0.053 NOT TAKEN SPILLED 0.044 0.045 NOT TAKEN 0.039 0.044 0.040 0.046 NOT TAKEN 0.041 0.043 0.038 0.044 SPILLED 0.041 0.041 0.045 0.036 SPILLED 0.040 0.038 0.041 SPILLED SPILLED 0.040 0.047 SPILLED NOT TAKEN 0.045 0.048 NOT TAKEN SPILLED 0.043 0.043 NOT TAKEN 0.040 0.045 0.043 0.047 NOT TAKEN 0.045 0.043 0.043 0.046 SPILLED 0.039 0.042 0.043 0.046 0.048 0.037 0.039 0.039 SPILLED 0.045 0.040 0.043 0.046 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED SPILLED 0.038 NOT TAKEN 0.040 0.040 0.036 0.046 NOT TAKEN 0.037 0.037 0.040 0.039 SPILLED 0.038 SPILLED 0.037 0.040 SPILLED 0.034 0.036 0.036 SPILLED SPILLED 0.035 0.045 SPILLED NOT TAKEN SPILLED SPILLED NOT TAKEN 0.041 0.039 0.037 NOT TAKEN 0.036 0.038 0.038 0.039 NOT TAKEN 0.038 0.038 0.037 0.039 0.038 0.036 0.042 0.038 0.040 0.040 0.034 0.035 0.036 SPILLED 0.041 0.037 0.037 0.037 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED SPILLED 0.038 NOT TAKEN 0.034 0.039 0.038 SPILLED NOT TAKEN 0.038 0.037 SPILLED 0.037 0.037 0.033 SPILLED 0.038 0.041 SPILLED 0.031 0.036 0.038 SPILLED SPILLED 0.035 SPILLED 0.035 NOT TAKEN 0.038 SPILLED NOT TAKEN 0.036 0.035 0.035 NOT TAKEN 0.033 0.035 0.032 0.033 NOT TAKEN 0.037 0.034 0.035 0.035 0.036 0.032 SPILLED 0.034 0.040 0.034 0.030 0.033 0.037 0.033 0.035 0.035 0.033 0.037 NOT TAKEN 0.035 SPILLED NOT TAKEN SPILLED 0.035 0.034 NOT TAKEN 0.032 0.033 0.029 0.033 NOT TAKEN 0.032 0.035 0.035 0.035 0.031 0.031 0.039 0.031 0.033 0.033 0.032 0.032 SPILLED SPILLED 0.034 0.033 0.031 0.033 NOT TAKEN 1442 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0.054 0.047 0.051 0.051 0.049 0.056 0.046 0.054 0.053 0.051 0.051 0.053 0.053 0.056 0.048 0.057 0.054 0.051 0.047 0.067 0.053 0.049 0.047 0.053 0.055 0.046 0.054 0.046 0.048 0.050 0.046 0.051 0.047 SPILLED SPILLED 0.048 0.051 0.054 0.051 0.051 SPILLED SPILLED 0.049 0.046 0.048 0.047 0.044 0.051 0.046 0.046 0.048 0.045 0.048 0.046 SPILLED 0.044 0.047 0.049 0.051 0.044 0.044 0.055 0.051 0.046 0.040 0.045 0.044 0.040 0.043 0.043 0.043 0.042 0.044 0.045 SPILLED NOT TAKEN 0.045 0.045 0.045 0.045 0.040 0.043 TECH SPILL 0.045 0.043 0.039 0.043 0.047 0.039 0.048 0.043 0.047 0.044 0.041 0.045 0.037 NOT TAKEN SPILLED 0.039 0.042 0.044 0.039 0.045 SPILLED 0.041 0.038 0.035 0.040 0.042 0.037 0.042 0.035 0.042 0.039 0.039 0.043 SPILLED NOT TAKEN 0.044 0.039 0.042 0.043 0.041 0.042 0.063 0.045 0.038 0.037 0.039 0.049 0.037 0.044 0.034 0.039 0.040 0.037 0.038 0.036 NOT TAKEN 0.041 0.036 0.040 0.039 0.035 0.038 SPILLED 0.040 0.039 0.033 0.040 0.041 0.034 0.037 0.033 0.036 0.035 0.033 0.039 0.033 NOT TAKEN SPILLED 0.036 0.035 0.037 0.036 0.037 SPILLED SPILLED 0.035 0.034 0.035 SPILLED 0.032 0.038 0.032 0.037 0.032 0.033 0.033 0.031 NOT TAKEN SPILLED 0.032 0.031 0.037 0.033 0.036 SPILLED 0.037 0.032 0.032 0.036 0.036 0.033 0.037 0.031 0.034 0.033 0.033 0.035 0.032 NOT TAKEN 0.033 0.030 0.032 0.036 SPILLED 0.037 SPILLED 0.037 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 SPILLED 0.222 0.210 0.202 0.231 0.208 0.198 SPILLED 0.183 0.188 0.178 0.201 0.192 0.182 0.226 0.173 0.181 0.168 0.200 0.176 0.174 SPILLED SPILLED 0.153 0.166 0.203 NOT TAKEN 0.170 SPILLED SPILLED 0.176 0.151 0.158 NOT TAKEN 0.153 SPILLED SPILLED 0.165 0.146 0.161 NOT TAKEN 0.138 0.156 0.141 0.142 0.132 0.151 NOT TAKEN 0.143 0.141 SPILLED 0.145 0.142 0.152 NOT TAKEN 0.151 0.136 0.135 0.142 0.133 NOT TAKEN NOT TAKEN 0.136 0.134 0.136 0.132 0.124 NOT TAKEN NOT TAKEN SPILLED 1443 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 0.212 SPILLED SPILLED 0.220 0.209 0.287 0.227 0.219 0.186 SPILLED 0.221 0.230 0.241 0.226 SPILLED SPILLED SPILLED 0.211 0.164 0.200 0.208 0.207 0.221 0.222 0.195 0.174 0.207 0.210 0.201 0.191 0.188 0.211 SPILLED SPILLED 0.202 0.190 SPILLED 0.203 0.193 0.177 SPILLED 0.213 0.190 0.207 0.189 SPILLED 0.204 0.220 0.198 0.186 0.205 0.194 0.190 0.225 0.207 0.206 0.189 0.188 0.193 0.195 0.181 0.184 0.219 SPILLED 0.202 0.177 0.179 0.175 0.240 0.176 0.149 SPILLED 0.195 0.264 0.202 0.179 0.175 0.182 SPILLED 0.187 0.216 0.180 0.182 SPILLED 0.208 0.193 0.170 0.178 0.188 0.201 0.178 0.173 0.170 0.181 SPILLED SPILLED 0.196 0.151 0.185 0.174 0.177 NOT TAKEN SPILLED NOT TAKEN 0.176 0.187 0.163 0.185 SPILLED 0.214 SPILLED 0.221 0.193 SPILLED SPILLED 0.173 SPILLED 0.178 0.157 0.175 0.171 0.179 0.171 0.163 0.187 SPILLED 0.173 SPILLED 0.165 0.176 0.167 0.168 NOT TAKEN 0.173 NOT TAKEN 0.169 0.169 0.152 0.163 SPILLED 0.179 0.157 0.189 0.177 0.169 SPILLED 0.168 0.165 0.170 0.162 0.161 0.151 0.178 0.170 SPILLED 0.163 SPILLED SPILLED SPILLED 0.147 0.147 0.165 0.157 NOT TAKEN SPILLED NOT TAKEN SPILLED 0.166 SPILLED 0.141 SPILLED 0.161 SPILLED 0.168 0.187 0.157 SPILLED SPILLED 0.157 0.148 SPILLED 0.157 0.166 0.156 0.172 SPILLED 0.163 SPILLED 0.144 0.144 0.128 0.145 0.153 0.141 NOT TAKEN SPILLED NOT TAKEN 0.145 0.152 0.126 0.146 0.149 SPILLED 0.149 0.160 0.161 0.140 SPILLED 0.160 0.145 0.147 SPILLED 0.148 0.143 0.144 0.148 0.150 0.152 SPILLED SPILLED 0.154 0.149 SPILLED 0.143 0.150 NOT TAKEN SPILLED NOT TAKEN 0.147 0.150 0.133 0.127 0.145 0.133 SPILLED 0.149 0.160 0.137 0.153 SPILLED 0.140 0.137 0.139 0.144 0.151 0.143 0.146 0.141 0.145 SPILLED SPILLED 0.155 0.125 0.137 0.136 0.127 NOT TAKEN SPILLED NOT TAKEN 0.147 0.144 0.124 0.129 0.128 0.129 SPILLED 0.148 0.157 0.131 SPILLED 0.139 0.128 0.130 SPILLED 0.134 SPILLED SPILLED 0.137 0.145 0.145 SPILLED SPILLED 0.156 0.137 0.119 0.138 0.139 NOT TAKEN SPILLED NOT TAKEN SPILLED 0.145 0.116 0.121 0.145 0.133 0.152 0.146 SPILLED 0.136 SPILLED SPILLED 0.139 0.134 SPILLED 0.139 0.139 0.148 0.131 0.144 1444 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 0.206 SPILLED 0.221 0.207 SPILLED 0.195 SPILLED 0.192 0.219 0.251 0.224 SPILLED 0.222 0.205 0.217 0.218 SPILLED 0.222 0.226 0.227 0.207 0.198 0.207 0.191 0.205 0.187 SPILLED 0.186 0.212 0.210 0.202 0.205 0.223 SPILLED 0.221 0.181 0.203 SPILLED 0.301 0.204 0.204 0.207 0.196 0.195 0.180 0.189 0.216 0.187 SPILLED 0.193 0.245 SPILLED 0.190 0.227 0.227 0.289 0.222 0.178 0.186 SPILLED SPILLED SPILLED 0.176 0.189 0.174 0.201 SPILLED 0.185 NOT TAKEN 0.156 SPILLED 0.149 0.187 0.163 0.178 SPILLED 0.191 SPILLED 0.189 0.180 0.172 0.178 NOT TAKEN 0.190 0.226 0.197 0.164 0.205 SPILLED 0.162 NOT TAKEN 0.168 SPILLED 0.171 0.190 0.193 0.174 SPILLED 0.183 SPILLED SPILLED 0.175 0.178 0.159 NOT TAKEN 0.154 0.184 0.175 0.166 0.188 SPILLED 0.154 NOT TAKEN 0.153 SPILLED 0.146 0.076 0.153 0.164 SPILLED 0.171 SPILLED SPILLED 0.148 0.166 0.142 NOT TAKEN 0.200 SPILLED 0.168 0.149 0.174 SPILLED 0.138 NOT TAKEN 0.144 SPILLED 0.125 0.151 0.148 0.150 0.159 0.155 SPILLED 0.167 0.142 0.159 0.146 NOT TAKEN SPILLED 0.150 SPILLED 0.141 0.171 SPILLED 0.150 NOT TAKEN 0.145 0.173 0.133 0.167 0.150 0.136 0.183 0.152 SPILLED 0.161 0.144 0.142 SPILLED NOT TAKEN SPILLED 0.138 0.162 SPILLED 0.169 SPILLED 0.148 NOT TAKEN 0.132 SPILLED 0.137 SPILLED SPILLED 0.142 0.176 0.150 0.262 0.174 0.124 0.144 0.131 NOT TAKEN 0.142 0.122 0.148 0.157 SPILLED SPILLED SPILLED NOT TAKEN 0.135 SPILLED 0.137 0.136 0.141 0.129 SPILLED 0.151 SPILLED 0.158 0.133 0.138 0.141 NOT TAKEN 0.132 0.125 0.160 0.145 SPILLED GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 0.567 0.560 0.513 SPILLED 0.570 0.569 SPILLED SPILLED 0.457 SPILLED 0.473 SPILLED 0.554 0.491 0.462 0.493 0.461 0.442 SPILLED SPILLED 0.415 0.456 0.453 0.426 SPILLED SPILLED 0.441 SPILLED 0.427 0.421 SPILLED SPILLED 0.372 SPILLED 0.380 0.380 0.394 SPILLED 0.408 SPILLED 0.352 0.363 0.412 SPILLED 0.406 SPILLED SPILLED 0.343 0.368 SPILLED 0.399 SPILLED 0.371 0.363 0.478 SPILLED 0.363 SPILLED SPILLED 0.330 1445 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 0.643 0.569 0.583 0.557 0.483 0.514 0.503 0.539 0.576 0.488 0.495 0.525 0.568 0.606 0.502 0.565 0.591 0.558 0.534 0.557 0.487 0.570 0.572 0.581 0.576 SPILLED 0.565 0.528 0.547 0.597 SPILLED 0.509 0.493 0.506 0.597 0.458 0.472 0.488 0.496 0.502 0.424 0.437 SPILLED 0.605 SPILLED 0.434 0.531 0.515 0.563 0.480 0.498 0.435 0.531 0.679 0.503 0.469 SPILLED 0.488 0.472 SPILLED SPILLED 0.589 0.525 0.464 0.496 0.507 0.439 0.458 0.429 0.476 0.503 0.440 0.495 0.469 0.488 0.527 0.436 0.504 0.499 0.539 0.489 0.563 0.433 0.486 0.467 0.457 0.472 SPILLED 0.466 0.451 0.477 SPILLED 0.606 0.457 NOT TAKEN 0.432 0.422 0.379 0.457 SPILLED 0.430 0.493 0.380 NOT TAKEN 0.401 0.424 NOT TAKEN 0.397 SPILLED 0.469 0.396 NOT TAKEN 0.452 0.379 0.483 0.498 0.407 0.411 SPILLED 0.424 NOT TAKEN 0.425 0.415 0.436 0.433 NOT TAKEN 0.426 TECH SPILL 0.381 0.418 0.396 0.405 0.439 SPILLED NOT TAKEN 0.423 0.403 NOT TAKEN 0.378 SPILLED 0.479 SPILLED NOT TAKEN 0.464 0.363 0.483 0.437 0.444 0.411 SPILLED 0.498 NOT TAKEN 0.486 0.424 SPILLED 0.397 NOT TAKEN 0.374 0.389 0.374 0.380 0.342 0.372 0.421 SPILLED NOT TAKEN 0.361 0.350 NOT TAKEN 0.366 SPILLED 0.405 0.450 NOT TAKEN SPILLED 0.352 0.436 0.406 0.363 0.395 SPILLED 0.404 NOT TAKEN 0.383 0.352 0.405 0.385 NOT TAKEN 0.380 0.400 0.354 0.386 0.326 0.387 0.418 SPILLED NOT TAKEN 0.349 0.341 NOT TAKEN 0.378 SPILLED 0.412 0.394 NOT TAKEN 0.419 0.361 0.403 0.416 0.331 0.362 0.402 0.399 NOT TAKEN SPILLED 0.357 0.397 0.350 NOT TAKEN 0.352 0.355 0.352 0.371 0.314 0.400 0.412 SPILLED NOT TAKEN 0.360 0.336 NOT TAKEN 0.367 SPILLED 0.392 0.378 NOT TAKEN 0.378 0.333 0.408 0.463 0.333 0.375 SPILLED 0.383 NOT TAKEN 0.339 SPILLED SPILLED 0.368 NOT TAKEN 0.343 0.329 0.317 0.345 0.311 0.388 SPILLED 0.369 NOT TAKEN 0.356 0.340 NOT TAKEN 0.360 SPILLED 0.388 0.379 NOT TAKEN 0.378 0.308 0.399 0.328 0.337 0.340 SPILLED 0.401 NOT TAKEN 0.345 SPILLED 0.331 0.375 NOT TAKEN 0.343 0.314 SPILLED 0.332 0.336 0.368 0.383 0.341 NOT TAKEN 0.340 0.299 NOT TAKEN 0.344 0.414 0.348 0.389 NOT TAKEN 0.383 0.304 0.398 0.350 0.299 0.380 0.347 0.344 NOT TAKEN SPILLED 0.344 SPILLED 1446 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.565 0.586 SPILLED 0.519 0.566 0.591 0.658 0.598 0.650 0.487 0.588 0.534 0.609 0.486 0.595 0.537 0.544 0.706 0.506 0.549 0.551 0.534 SPILLED 0.477 0.484 SPILLED 0.460 0.495 0.474 0.663 0.485 SPILLED 0.467 SPILLED 0.492 0.560 SPILLED 0.528 0.528 0.517 0.527 0.467 0.483 0.508 0.477 0.602 0.459 0.459 SPILLED 0.413 0.501 0.460 0.520 0.479 0.568 0.462 0.527 0.461 0.504 0.432 0.482 0.467 0.488 0.478 0.429 SPILLED 0.449 0.453 SPILLED 0.416 0.569 SPILLED 0.434 SPILLED 0.431 0.654 0.461 SPILLED 0.475 SPILLED 0.410 0.489 0.412 0.442 0.408 0.463 0.414 0.404 0.444 0.425 0.433 SPILLED 0.402 0.432 SPILLED 0.352 0.428 0.420 0.433 0.483 0.511 0.439 0.410 0.382 0.441 0.405 0.459 0.454 0.476 0.451 0.408 0.412 0.410 0.417 0.497 0.352 SPILLED SPILLED 0.363 0.428 SPILLED 0.502 SPILLED 0.459 0.405 0.402 0.344 SPILLED 0.409 0.403 0.396 SPILLED 0.351 0.385 0.392 0.378 0.368 0.456 0.372 SPILLED 0.368 0.336 0.398 0.395 0.381 SPILLED 0.425 0.408 0.365 0.336 0.410 SPILLED 0.393 0.346 0.461 0.370 0.368 0.387 0.385 0.379 SPILLED 0.330 0.387 0.355 0.305 0.366 0.373 0.358 SPILLED 0.390 SPILLED SPILLED 0.352 0.390 0.365 0.402 0.381 SPILLED 0.358 0.334 0.357 0.366 0.372 SPILLED 0.338 0.349 0.317 0.330 0.364 0.378 0.367 0.349 0.374 SPILLED SPILLED 0.310 0.392 0.347 0.370 0.358 0.426 0.331 0.334 0.318 0.360 0.340 SPILLED 0.374 0.377 0.349 0.322 0.374 0.347 0.360 0.348 0.391 SPILLED 0.379 0.303 0.382 0.327 SPILLED 0.328 SPILLED 0.375 0.364 0.327 0.368 0.345 SPILLED GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 2.224 2.139 SPILLED 2.022 1.991 2.088 1.969 SPILLED 1.937 2.001 1.889 1.709 SPILLED 1.749 1.837 1.644 1.581 1.833 NOT TAKEN 1.675 1.546 1.546 1.745 NOT TAKEN 1.876 1.408 1.407 1.575 NOT TAKEN 1.534 1.409 1.350 1.631 NOT TAKEN 1.536 1.317 1.288 1.621 NOT TAKEN 1.555 1.217 1.199 1.562 NOT TAKEN 1.432 SPILLED 1.322 1.645 NOT TAKEN 1.487 1447 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 2.068 2.117 1.953 2.114 2.296 SPILLED 2.121 2.027 SPILLED 2.035 1.975 2.101 1.803 2.344 2.145 2.190 2.122 2.194 2.016 2.399 2.035 2.331 1.927 2.492 2.314 2.238 2.307 2.095 SPILLED 2.131 2.060 1.815 1.932 1.981 1.808 2.016 1.869 2.040 2.122 SPILLED 2.101 1.795 1.801 1.915 2.159 2.002 1.862 1.816 1.826 1.648 1.980 1.694 2.348 1.783 2.269 2.139 SPILLED 1.891 2.006 2.145 1.888 1.671 1.691 SPILLED 1.845 1.745 1.788 1.751 SPILLED 1.701 2.110 1.954 SPILLED 1.714 1.616 2.045 1.743 1.737 1.660 1.732 1.550 1.878 1.525 2.071 1.619 SPILLED 2.043 2.104 1.811 SPILLED 1.984 SPILLED 1.546 1.659 1.656 1.727 1.695 1.621 1.510 1.553 NOT TAKEN SPILLED 1.813 1.521 1.764 1.642 1.858 1.819 1.551 1.465 1.587 1.524 1.721 1.531 1.689 1.547 1.745 1.729 SPILLED NOT TAKEN SPILLED NOT TAKEN 1.842 1.526 SPILLED SPILLED 1.678 1.621 SPILLED SPILLED 1.683 NOT TAKEN SPILLED 1.783 1.516 1.530 1.542 1.756 1.707 1.531 1.461 1.541 1.506 1.761 1.493 1.864 1.562 1.827 1.836 2.084 NOT TAKEN 1.766 NOT TAKEN SPILLED SPILLED 1.516 SPILLED 1.455 1.488 1.568 SPILLED 1.317 NOT TAKEN SPILLED 1.684 1.391 1.302 1.438 1.641 1.458 1.468 1.306 1.339 1.473 1.501 1.375 1.697 1.413 SPILLED 1.606 1.522 NOT TAKEN SPILLED NOT TAKEN 1.756 SPILLED SPILLED 1.471 1.587 1.453 1.481 1.509 1.325 NOT TAKEN 1.512 SPILLED 1.805 1.513 1.483 1.547 1.592 1.341 1.328 SPILLED 1.340 1.519 1.340 1.669 1.431 1.592 1.554 SPILLED NOT TAKEN 1.666 NOT TAKEN 1.635 1.673 SPILLED 1.458 1.429 1.303 1.480 1.353 SPILLED NOT TAKEN 1.300 1.656 1.330 1.511 1.366 1.494 1.522 1.327 1.229 1.392 1.349 1.462 1.411 1.493 1.363 1.485 1.609 1.744 NOT TAKEN SPILLED NOT TAKEN SPILLED 1.392 1.387 SPILLED 1.479 1.404 1.306 1.337 1.371 NOT TAKEN 1.411 1.398 1.290 1.289 1.405 1.490 1.415 1.300 1.322 1.362 1.213 1.448 1.329 1.419 1.370 1.520 1.369 SPILLED NOT TAKEN 1.421 NOT TAKEN 1.597 SPILLED 1.414 1.367 1.450 1.439 1.485 1.366 1.268 NOT TAKEN 1.613 1.636 1.290 1.369 1.326 1.398 1.407 1.209 1.401 1.387 1.301 1.349 1.457 1.535 1.313 1.598 1.468 1.459 NOT TAKEN SPILLED NOT TAKEN 1.441 1.463 1448 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 2.107 2.215 2.124 2.491 2.087 2.178 SPILLED 2.258 2.347 2.169 2.172 2.201 2.114 2.395 2.118 2.235 2.020 2.303 2.187 2.025 1.893 2.449 2.087 2.075 SPILLED 2.035 2.094 2.099 2.127 2.207 2.043 1.915 1.875 SPILLED 2.299 1.779 2.107 SPILLED 2.024 2.276 1.944 1.884 1.950 1.825 2.125 SPILLED 2.056 1.926 1.957 1.992 1.783 2.004 2.461 1.940 1.945 SPILLED 1.749 2.049 SPILLED 2.049 1.949 1.852 1.721 1.579 2.004 1.970 1.621 SPILLED 2.020 1.748 2.088 1.770 2.006 1.692 1.716 1.901 SPILLED 2.004 SPILLED 1.609 1.815 1.666 1.704 1.993 1.773 1.855 SPILLED 1.639 1.951 SPILLED 1.787 1.855 1.742 1.681 1.480 1.867 1.881 1.416 1.583 SPILLED 1.640 1.494 1.549 1.622 1.543 NOT TAKEN 1.740 1.693 1.648 1.564 SPILLED 1.636 1.644 1.483 1.887 1.787 1.684 SPILLED SPILLED 1.419 1.777 1.533 1.684 1.607 1.651 1.565 2.032 1.823 1.606 1.513 SPILLED 1.669 1.697 1.637 1.732 1.706 NOT TAKEN 1.807 1.664 1.665 1.548 SPILLED 1.734 1.505 1.596 1.850 1.654 1.825 SPILLED 1.502 1.609 1.824 1.642 1.668 1.564 1.578 1.526 SPILLED 1.557 1.474 1.596 SPILLED 1.507 1.651 1.479 1.672 1.413 NOT TAKEN 1.551 SPILLED 1.637 1.527 1.575 1.569 1.487 1.370 1.752 1.456 1.505 SPILLED 1.426 1.454 1.555 1.403 1.454 1.435 1.517 1.631 SPILLED 1.516 1.452 SPILLED SPILLED 1.480 1.803 1.515 1.619 1.335 NOT TAKEN 1.495 SPILLED 1.458 1.419 1.503 1.462 SPILLED 1.319 1.605 1.467 1.442 SPILLED 1.489 SPILLED SPILLED 1.530 1.468 1.495 1.376 1.450 1.828 1.579 1.423 1.415 SPILLED 1.443 1.913 1.422 1.467 1.466 NOT TAKEN 1.479 1.445 SPILLED 1.286 SPILLED 1.358 1.351 1.376 1.480 1.396 SPILLED SPILLED 1.283 SPILLED SPILLED 1.314 1.362 1.373 1.365 1.405 1.849 1.611 1.520 1.385 SPILLED 1.398 1.591 1.332 1.503 1.375 NOT TAKEN 1.402 SPILLED 1.522 1.293 1.438 1.385 1.216 1.162 1.390 1.417 1.524 SPILLED SPILLED 1.504 SPILLED 1.390 1.337 1.650 1.295 1.298 SPILLED 1.545 1.359 1.306 SPILLED 1.394 SPILLED 1.319 1.487 1.393 NOT TAKEN 1.351 1.352 SPILLED 1.337 1.328 1.324 1.255 1.269 SPILLED 1.285 SPILLED SPILLED 1.431 SPILLED SPILLED 1.349 1.302 1.528 1449 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93178 C93179 C93180 SPILLED 2.080 SPILLED 1.935 1.831 2.033 1.859 1.659 SPILLED 1.763 1.573 1.555 1.552 1.602 1.605 1.474 1.464 1.439 1.464 1.410 1.385 1.320 1.376 1.408 1.373 1.377 SPILLED 1.323 SPILLED 1.356 GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 2.207 2.118 2.137 2.243 SPILLED 2.049 2.136 2.299 SPILLED 2.368 2.107 1.933 2.182 1.981 2.399 2.230 1.905 2.177 2.073 2.390 SPILLED 1.950 2.191 2.355 2.039 1.953 1.952 1.815 1.887 2.434 1.944 1.985 1.861 SPILLED 1.865 2.045 1.682 2.104 1.801 SPILLED 2.349 1.675 1.930 1.711 2.006 2.028 1.993 1.900 2.012 SPILLED 1.846 1.900 1.792 1.957 SPILLED 1.840 1.683 1.822 SPILLED 1.773 1.552 1.553 1.896 1.864 1.796 SPILLED 1.595 1.817 1.690 1.949 1.929 1.710 1.602 1.967 1.945 1.655 1.690 1.557 1.814 SPILLED 1.603 1.835 1.380 SPILLED 1.553 1.621 1.751 1.691 1.759 1.533 SPILLED 1.542 1.774 1.508 1.993 1.655 1.629 1.733 1.732 1.488 1.802 1.682 1.549 1.739 SPILLED 1.557 1.496 1.541 SPILLED 1.634 1.514 1.579 1.645 1.634 1.509 SPILLED 1.436 1.660 1.506 1.992 1.618 1.571 1.505 1.751 1.486 1.665 1.393 1.524 1.544 1.685 1.635 1.501 1.397 1.566 1.478 1.423 1.464 1.583 1.531 1.390 SPILLED 1.391 1.481 1.453 1.573 1.384 SPILLED 1.649 1.609 1.391 1.544 1.478 1.362 1.583 1.526 1.402 1.518 1.412 1.602 1.460 1.448 1.517 1.446 1.573 1.343 1.606 1.418 1.403 1.298 SPILLED 1.315 1.263 1.439 1.521 1.457 1.546 1.304 1.296 1.504 1.693 1.396 1.341 1.366 1.544 1.350 1.429 1.323 1.350 1.374 SPILLED 1.492 1.322 1.247 1.347 SPILLED SPILLED SPILLED SPILLED 1.463 1.332 1.507 1.426 1.283 1.410 1.532 1.449 1.384 1.314 1.526 1.415 1.251 1.247 1.237 1.473 1.663 1.543 1.377 1.366 1.292 2.433 1.429 SPILLED 1.415 1.455 1.338 1.494 1.293 1.399 1.404 1.634 1.569 1.306 1.304 1.497 1.437 1.381 1.355 1.472 1.705 1.328 1.435 1.381 1.395 1.297 SPILLED 1.333 SPILLED 1.303 1.562 1.365 1450 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 1 2 3 4 5 6 7 8 9 10 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 2.364 2.324 1.992 1.931 2.276 1.862 2.049 2.016 2.111 2.639 2.076 SPILLED 2.435 2.040 SPILLED 1.910 2.120 1.756 1.798 1.815 SPILLED 2.052 1.811 1.862 1.931 1.745 SPILLED 1.949 1.892 SPILLED 1.775 1.855 1.733 SPILLED 1.689 1.787 1.830 1.748 1.651 SPILLED 1.707 SPILLED 2.079 1.811 SPILLED 1.554 1.665 1.429 SPILLED 2.035 1.653 1.796 1.575 SPILLED 1.618 1.565 SPILLED 1.667 1.613 SPILLED 1.765 1.653 1.487 SPILLED 1.629 1.596 1.722 1.520 SPILLED 1.603 1.584 SPILLED 1.679 1.579 SPILLED SPILLED 1.416 1.211 SPILLED SPILLED 1.399 1.479 1.414 SPILLED 1.469 1.407 1.628 SPILLED SPILLED SPILLED 1.483 1.498 1.429 1.312 1.401 1.416 1.572 1.343 1.429 1.380 1.363 1.498 SPILLED SPILLED SPILLED SPILLED 1.550 1.279 SPILLED 1.396 1.422 1.456 1.281 1.377 1.398 1.310 1.326 SPILLED 1.346 SPILLED SPILLED 1.407 1.213 1.423 1.372 1.561 1.481 1.305 1.432 1.310 1.376 1.294 SPILLED 1.245 SPILLED 1.571 1.380 1.262 SPILLED 1.295 1.499 1.520 1.311 1.417 1.312 1.277 1.345 SPILLED SPILLED SPILLED 1451 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 0.031 0.028 0.025 NOT TAKEN NOT TAKEN 0.025 0.026 0.027 0.026 0.026 0.024 0.025 0.027 0.029 CNTMD 0.026 0.025 SPILLED 0.027 0.026 0.026 0.024 0.025 0.028 TECH SPILL 0.028 SPILLED 0.026 0.027 0.027 0.024 0.030 0.028 0.025 NOT TAKEN NOT TAKEN 0.025 0.026 0.026 0.025 0.027 0.024 0.025 0.026 0.030 CNTMD 0.026 0.025 0.026 0.025 0.027 0.025 0.024 0.024 0.028 0.025 0.027 0.028 0.026 0.027 0.025 0.025 0.029 0.027 0.024 NOT TAKEN NOT TAKEN 0.025 0.025 0.026 0.025 0.026 0.024 0.024 0.024 0.029 0.027 0.026 0.024 0.026 0.024 0.026 0.024 0.023 0.023 0.026 0.024 0.027 0.028 0.025 0.026 0.025 0.024 0.027 0.027 0.023 NOT TAKEN NOT TAKEN 0.024 0.024 0.026 0.023 0.025 0.023 0.024 0.022 0.027 0.024 0.026 0.024 0.025 0.024 0.025 0.024 0.024 NOT TAKEN 0.026 0.024 0.026 0.027 0.022 0.026 NOT TAKEN 0.023 0.027 0.026 0.023 NOT TAKEN NOT TAKEN 0.024 0.023 0.024 0.023 0.025 0.022 0.024 0.027 0.029 CNTMD 0.023 0.024 0.027 0.024 0.025 0.023 0.023 NOT TAKEN 0.027 0.026 0.025 0.028 0.024 0.025 NOT TAKEN 0.023 0.025 0.026 0.023 NOT TAKEN NOT TAKEN 0.024 0.024 0.025 0.022 0.025 0.022 0.024 0.025 0.027 0.024 0.025 0.024 0.026 0.023 0.024 0.024 0.024 NOT TAKEN 0.026 0.024 0.025 0.029 0.024 0.025 NOT TAKEN 0.024 0.023 0.025 0.021 NOT TAKEN NOT TAKEN 0.025 0.023 0.024 0.023 0.025 0.023 0.024 0.023 0.026 0.025 0.025 0.024 SPILLED 0.023 0.022 0.024 0.023 NOT TAKEN 0.025 0.022 0.026 0.027 0.025 0.024 NOT TAKEN 0.023 0.021 0.022 0.020 NOT TAKEN NOT TAKEN 0.021 0.020 0.022 0.021 0.021 0.020 0.022 0.022 0.024 0.023 0.021 0.022 0.023 0.022 0.022 0.022 0.022 NOT TAKEN 0.023 0.021 0.024 0.025 0.022 0.022 NOT TAKEN 0.022 0.019 0.021 0.019 NOT TAKEN NOT TAKEN 0.021 0.019 0.020 0.019 0.020 0.019 0.023 0.021 0.025 0.021 0.020 0.020 0.021 0.020 SPILLED 0.021 0.020 NOT TAKEN 0.022 0.021 0.022 0.025 0.022 0.022 NOT TAKEN 0.021 0.020 0.020 0.018 NOT TAKEN NOT TAKEN 0.019 0.018 0.019 0.018 0.019 0.019 0.021 0.020 0.023 0.019 0.020 0.018 0.020 0.019 0.018 0.019 0.018 NOT TAKEN 0.020 0.019 0.020 0.022 0.020 0.022 NOT TAKEN 0.020 1452 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 0.028 0.027 0.026 0.026 0.029 0.028 SPILLED 0.026 NOT TAKEN 0.025 0.026 NOT TAKEN SPILLED 0.027 0.031 0.026 NOT TAKEN 0.028 0.026 SPILLED 0.026 0.026 0.027 0.027 0.026 SPILLED 0.028 SPILLED 0.032 0.029 0.027 0.026 0.026 0.028 0.027 0.028 0.025 NOT TAKEN 0.025 0.024 NOT TAKEN SPILLED 0.027 0.029 0.026 NOT TAKEN 0.027 0.027 0.028 0.025 0.026 0.025 0.026 0.025 SPILLED SPILLED 0.028 0.031 0.028 0.024 0.025 0.024 0.026 0.026 0.025 0.025 NOT TAKEN 0.024 0.023 NOT TAKEN SPILLED 0.025 0.029 0.024 NOT TAKEN 0.026 0.030 0.027 0.023 0.024 0.025 0.024 0.024 SPILLED SPILLED 0.027 0.030 0.028 0.025 0.024 0.024 0.025 0.027 0.024 0.025 NOT TAKEN 0.024 0.021 NOT TAKEN SPILLED 0.025 NOT TAKEN 0.024 NOT TAKEN 0.026 0.028 NOT TAKEN 0.024 0.023 0.023 0.024 0.023 NOT TAKEN 0.028 0.024 0.029 0.027 0.023 0.024 0.024 0.024 0.025 0.015 0.025 NOT TAKEN 0.024 0.024 NOT TAKEN SPILLED 0.025 NOT TAKEN 0.024 NOT TAKEN 0.027 0.026 NOT TAKEN 0.023 0.024 0.025 0.025 0.023 NOT TAKEN SPILLED 0.026 0.030 0.026 0.025 0.024 0.024 0.026 0.025 0.021 0.025 NOT TAKEN 0.023 0.024 NOT TAKEN SPILLED 0.024 NOT TAKEN 0.023 NOT TAKEN 0.025 0.026 NOT TAKEN 0.024 0.025 0.025 0.024 0.019 NOT TAKEN 0.026 0.026 0.030 0.026 NOT TAKEN 0.023 0.021 0.025 0.024 0.025 0.025 NOT TAKEN 0.022 0.022 NOT TAKEN SPILLED 0.022 NOT TAKEN 0.022 NOT TAKEN 0.024 0.024 NOT TAKEN 0.022 0.023 0.023 0.021 0.023 NOT TAKEN 0.026 0.026 SPILLED 0.023 NOT TAKEN 0.021 0.021 0.022 0.023 0.022 0.023 NOT TAKEN 0.021 0.015 NOT TAKEN SPILLED 0.021 NOT TAKEN 0.021 NOT TAKEN 0.021 0.022 NOT TAKEN 0.022 0.021 0.021 0.020 0.022 NOT TAKEN 0.023 0.021 0.025 0.024 NOT TAKEN 0.020 0.019 0.019 0.019 0.020 0.023 NOT TAKEN 0.019 0.019 NOT TAKEN SPILLED 0.019 NOT TAKEN 0.019 NOT TAKEN 0.021 0.021 NOT TAKEN 0.019 0.020 0.020 0.019 0.018 NOT TAKEN 0.023 0.021 SPILLED 0.022 NOT TAKEN 0.019 0.017 0.019 0.020 0.019 0.021 NOT TAKEN 0.020 0.019 NOT TAKEN SPILLED 0.018 NOT TAKEN 0.018 NOT TAKEN 0.020 0.021 NOT TAKEN 0.018 0.018 0.020 0.017 SPILLED NOT TAKEN SPILLED 0.019 SPILLED 1453 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 0.110 0.100 0.106 0.119 SPILLED SPILLED 0.107 0.112 0.106 0.110 0.079 0.104 0.112 0.114 0.117 NOT TAKEN 0.103 0.105 0.119 NOT TAKEN 0.109 NOT TAKEN 0.104 0.110 SPILLED 0.101 SPILLED 0.106 0.108 0.111 0.098 0.108 0.096 0.104 0.115 0.115 0.112 0.108 SPILLED 0.104 0.112 0.099 0.101 0.107 0.111 0.119 NOT TAKEN 0.104 0.108 0.121 NOT TAKEN 0.110 NOT TAKEN 0.103 0.112 SPILLED 0.099 SPILLED 0.107 0.110 0.104 0.097 0.104 0.090 0.099 0.114 0.107 0.113 0.104 0.100 0.102 0.110 0.106 0.102 0.106 0.106 0.118 NOT TAKEN 0.101 0.103 0.121 NOT TAKEN 0.103 NOT TAKEN 0.101 0.105 SPILLED 0.098 0.105 0.103 0.105 0.103 0.098 0.101 0.089 0.098 0.119 0.105 0.099 0.105 0.104 0.100 NOT TAKEN 0.106 0.094 0.106 0.096 NOT TAKEN NOT TAKEN 0.097 0.107 0.117 NOT TAKEN 0.092 NOT TAKEN 0.101 0.107 0.121 0.091 0.101 0.099 0.098 0.106 0.088 0.100 0.106 0.095 0.119 0.100 SPILLED 0.105 0.095 0.098 NOT TAKEN 0.103 0.095 0.100 0.111 NOT TAKEN NOT TAKEN 0.101 0.107 0.117 NOT TAKEN 0.102 NOT TAKEN 0.095 0.104 SPILLED 0.093 0.102 0.099 0.108 0.102 0.104 0.105 0.090 0.100 0.105 0.100 0.109 0.103 0.100 0.101 NOT TAKEN 0.106 0.094 SPILLED 0.107 NOT TAKEN NOT TAKEN 0.100 0.105 0.113 NOT TAKEN 0.102 NOT TAKEN 0.091 0.099 SPILLED 0.091 0.097 0.093 0.101 0.103 0.101 0.097 0.086 0.099 0.111 0.095 0.100 0.102 0.091 0.093 NOT TAKEN 0.098 0.086 0.096 0.100 NOT TAKEN NOT TAKEN 0.094 0.103 0.113 NOT TAKEN 0.092 NOT TAKEN 0.093 0.093 0.107 0.085 0.099 0.093 0.096 0.115 0.091 0.091 0.085 0.089 0.100 0.093 0.089 0.095 0.091 0.094 NOT TAKEN 0.091 0.081 0.097 0.094 NOT TAKEN NOT TAKEN 0.089 0.094 0.097 NOT TAKEN 0.093 NOT TAKEN 0.082 0.094 0.103 0.079 0.089 0.081 0.084 0.108 0.087 0.089 0.074 0.084 0.087 0.083 0.084 0.088 0.078 0.083 NOT TAKEN 0.071 0.079 0.089 0.088 NOT TAKEN NOT TAKEN 0.082 0.088 0.091 NOT TAKEN 0.083 NOT TAKEN 0.078 0.085 0.099 0.074 0.080 0.079 0.083 0.095 0.081 0.081 0.070 0.076 0.082 0.080 0.082 0.085 0.075 0.075 NOT TAKEN 0.075 0.073 0.084 0.091 NOT TAKEN NOT TAKEN 0.080 0.082 0.090 NOT TAKEN 0.080 NOT TAKEN 0.075 0.082 0.091 0.071 0.079 0.076 0.079 SPILLED 0.073 1454 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 0.106 0.112 0.110 0.103 0.116 0.110 NOT TAKEN SPILLED 0.111 0.115 0.107 0.102 SPILLED 0.115 0.109 0.102 NOT TAKEN 0.110 0.107 0.107 0.106 0.108 0.117 0.105 0.100 0.108 SPILLED 0.104 0.099 0.104 0.112 0.107 0.099 0.118 0.106 NOT TAKEN 0.123 0.110 0.109 0.105 0.103 0.111 0.107 0.101 0.098 NOT TAKEN 0.104 0.111 0.105 0.103 0.105 0.064 0.097 0.099 0.104 0.121 0.093 0.097 0.108 0.113 0.102 0.103 0.113 0.106 NOT TAKEN 0.119 0.110 0.108 0.097 0.103 0.110 0.107 0.100 0.099 NOT TAKEN 0.105 0.104 0.097 0.103 0.103 0.105 0.098 0.099 0.103 0.119 0.097 0.097 NOT TAKEN 0.112 0.103 0.098 0.110 0.102 NOT TAKEN SPILLED 0.105 0.103 0.101 0.099 0.112 0.105 NOT TAKEN 0.101 NOT TAKEN 0.101 0.101 0.098 0.090 0.102 NOT TAKEN 0.101 0.101 0.099 SPILLED 0.099 0.095 NOT TAKEN 0.107 0.102 0.097 0.107 0.092 NOT TAKEN 0.122 0.105 0.104 0.100 0.096 0.112 0.103 NOT TAKEN 0.102 NOT TAKEN 0.103 0.100 0.097 0.101 0.100 NOT TAKEN 0.099 0.095 0.099 SPILLED 0.102 0.093 NOT TAKEN 0.106 0.103 0.096 0.113 0.095 NOT TAKEN 0.109 0.102 0.104 0.097 0.096 0.103 0.106 NOT TAKEN 0.104 NOT TAKEN 0.102 0.093 0.102 0.096 0.094 NOT TAKEN 0.095 0.097 0.091 0.113 0.099 0.088 NOT TAKEN 0.100 0.102 0.088 0.109 0.093 NOT TAKEN 0.099 0.099 0.097 0.086 0.091 0.097 0.100 NOT TAKEN 0.101 NOT TAKEN 0.096 0.090 0.091 0.096 0.097 NOT TAKEN 0.091 0.095 0.088 SPILLED 0.092 0.089 NOT TAKEN 0.097 0.090 0.083 0.115 0.082 NOT TAKEN 0.093 0.090 0.090 0.086 0.081 0.094 0.096 NOT TAKEN 0.094 NOT TAKEN 0.089 0.083 0.090 0.086 0.092 NOT TAKEN 0.083 0.089 0.085 SPILLED 0.090 0.077 NOT TAKEN 0.085 0.080 0.077 0.104 0.082 NOT TAKEN 0.085 0.088 0.085 0.076 0.074 0.079 0.089 NOT TAKEN 0.090 NOT TAKEN 0.086 0.079 SPILLED 0.079 0.083 NOT TAKEN 0.074 0.083 0.080 SPILLED 0.080 0.075 NOT TAKEN 0.080 0.082 0.075 SPILLED 0.078 NOT TAKEN 0.082 0.082 0.085 0.075 0.076 0.076 0.090 NOT TAKEN 0.085 NOT TAKEN 0.082 0.074 0.077 0.079 0.080 NOT TAKEN 0.076 0.076 0.074 0.097 0.076 0.074 1455 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 0.265 0.249 0.271 0.240 0.261 0.279 0.304 0.260 0.273 0.270 0.252 0.249 0.294 0.261 0.283 0.241 0.250 0.264 0.267 0.275 0.243 0.267 0.256 0.278 NOT TAKEN 0.242 0.269 NOT TAKEN 0.278 SPILLED 0.260 0.254 0.246 0.256 0.229 0.259 SPILLED 0.295 0.254 0.270 0.269 0.247 0.251 0.298 0.251 0.275 0.246 0.235 0.237 0.262 0.275 0.240 0.258 0.256 0.265 NOT TAKEN 0.247 0.275 NOT TAKEN 0.275 SPILLED 0.259 0.249 0.246 0.245 0.209 0.248 0.266 0.288 0.253 0.258 0.270 0.244 0.239 0.283 0.240 0.260 0.235 0.234 0.260 0.261 0.255 0.239 0.263 0.242 0.263 NOT TAKEN 0.240 0.257 NOT TAKEN 0.272 SPILLED 0.260 0.244 0.245 0.249 0.228 0.246 0.248 SPILLED 0.247 0.256 0.261 0.237 0.238 0.279 0.245 0.265 0.230 0.233 0.250 0.249 0.251 0.201 0.278 NOT TAKEN NOT TAKEN NOT TAKEN 0.204 0.258 NOT TAKEN NOT TAKEN SPILLED 0.246 0.257 0.234 0.252 0.234 0.244 0.249 0.260 0.243 0.248 0.257 0.227 0.236 0.264 0.235 0.266 0.231 0.236 0.256 0.260 0.247 0.239 0.273 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.256 NOT TAKEN NOT TAKEN SPILLED 0.251 0.251 0.229 0.242 0.239 0.247 0.245 0.272 0.226 0.247 0.263 0.226 0.232 0.273 0.236 0.251 0.216 0.232 0.249 0.247 0.238 0.241 0.257 NOT TAKEN NOT TAKEN NOT TAKEN 0.231 0.264 NOT TAKEN NOT TAKEN 0.287 0.262 0.244 0.209 0.221 0.225 0.227 0.243 0.269 0.226 0.179 0.262 0.227 0.219 0.252 0.222 0.248 0.207 0.225 0.230 0.231 0.226 0.219 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.220 0.245 NOT TAKEN NOT TAKEN SPILLED 0.230 0.222 0.195 0.211 0.195 0.214 0.236 0.249 0.198 0.227 0.228 0.200 0.205 0.223 0.201 0.228 0.193 0.206 0.207 0.217 0.208 0.204 0.233 NOT TAKEN NOT TAKEN NOT TAKEN 0.203 0.215 NOT TAKEN NOT TAKEN 0.254 0.227 0.206 0.193 0.194 0.192 0.213 SPILLED 0.223 0.183 0.203 0.248 0.197 0.201 0.206 0.193 0.218 0.194 0.200 0.201 0.207 0.209 0.193 0.219 NOT TAKEN NOT TAKEN NOT TAKEN 0.191 0.207 NOT TAKEN NOT TAKEN 0.239 0.222 0.199 0.180 0.195 0.187 0.199 0.186 0.224 0.183 0.224 SPILLED 0.187 0.206 0.198 0.179 0.197 0.190 0.201 0.199 0.193 0.194 0.189 0.209 NOT TAKEN NOT TAKEN NOT TAKEN 0.191 0.204 NOT TAKEN NOT TAKEN 0.224 0.204 1456 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 0.297 SPILLED SPILLED 0.268 0.264 0.264 0.271 0.268 SPILLED NOT TAKEN 0.282 0.283 NOT TAKEN 0.254 0.256 0.266 0.273 SPILLED 0.265 0.256 0.257 SPILLED NOT TAKEN 0.265 SPILLED 0.266 SPILLED SPILLED SPILLED 0.291 SPILLED SPILLED 0.256 0.253 0.281 0.266 0.269 0.262 NOT TAKEN 0.293 0.273 NOT TAKEN 0.253 0.258 0.257 0.265 0.297 0.259 0.250 0.248 0.254 NOT TAKEN 0.261 SPILLED 0.271 SPILLED 0.275 0.266 0.274 SPILLED 0.310 0.252 0.248 0.275 0.254 0.272 0.254 NOT TAKEN 0.281 0.275 NOT TAKEN 0.249 0.253 0.247 0.251 0.281 0.252 0.251 0.242 0.255 NOT TAKEN 0.252 0.225 0.258 SPILLED 0.241 0.257 NOT TAKEN SPILLED SPILLED 0.252 0.217 0.243 NOT TAKEN 0.254 0.257 NOT TAKEN 0.270 0.287 NOT TAKEN 0.249 0.246 0.252 0.252 0.269 0.248 0.225 0.230 0.253 NOT TAKEN 0.252 0.218 0.261 SPILLED 0.263 0.254 NOT TAKEN SPILLED SPILLED 0.242 0.272 0.248 NOT TAKEN 0.235 0.257 NOT TAKEN 0.267 0.286 NOT TAKEN 0.244 0.245 0.251 0.255 0.267 0.240 0.249 0.227 0.245 NOT TAKEN 0.244 0.229 0.255 SPILLED 0.263 0.252 NOT TAKEN 0.256 0.294 0.243 0.249 0.261 NOT TAKEN 0.237 0.252 NOT TAKEN 0.273 0.260 NOT TAKEN 0.254 0.231 0.241 0.229 0.273 0.236 0.236 0.220 0.239 NOT TAKEN 0.249 0.222 0.243 SPILLED 0.252 0.237 NOT TAKEN SPILLED 0.258 0.228 0.222 0.244 NOT TAKEN 0.229 0.226 NOT TAKEN 0.247 0.283 NOT TAKEN 0.223 0.238 0.235 0.227 0.270 0.229 0.253 0.199 0.235 NOT TAKEN 0.227 0.220 0.236 0.277 0.248 0.239 NOT TAKEN 0.242 0.245 0.204 0.235 0.224 NOT TAKEN 0.213 0.223 NOT TAKEN 0.218 0.255 NOT TAKEN 0.206 0.223 0.210 0.234 0.260 0.205 0.222 0.189 0.200 NOT TAKEN 0.213 0.205 0.223 0.258 0.222 0.215 NOT TAKEN SPILLED SPILLED 0.194 0.215 0.197 NOT TAKEN 0.197 0.212 NOT TAKEN 0.213 0.248 NOT TAKEN 0.201 0.204 0.204 0.208 0.228 0.193 SPILLED 0.180 0.191 NOT TAKEN 0.189 0.193 0.216 0.231 0.225 0.205 NOT TAKEN SPILLED 0.209 0.190 0.203 0.203 NOT TAKEN 0.190 0.194 NOT TAKEN 0.203 0.228 NOT TAKEN 0.202 0.190 0.198 0.205 0.222 0.197 SPILLED 0.177 0.192 NOT TAKEN 0.201 0.186 0.200 0.220 0.206 0.194 1457 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 0.991 NOT TAKEN 0.884 1.033 1.068 1.194 1.127 0.978 NOT TAKEN 1.073 1.077 1.170 1.006 1.206 1.064 1.063 1.154 0.999 1.080 1.004 0.976 0.998 1.019 1.043 1.115 1.017 1.132 1.019 NOT TAKEN SPILLED 1.020 0.902 NOT TAKEN SPILLED 1.107 1.051 1.276 1.099 0.962 NOT TAKEN 1.144 1.045 1.139 1.043 1.260 1.006 1.078 1.131 0.977 1.088 0.998 SPILLED 1.073 1.027 1.077 1.108 1.016 1.143 1.038 NOT TAKEN 1.173 0.958 0.994 NOT TAKEN 1.137 1.035 1.037 1.204 1.047 0.951 NOT TAKEN 1.055 1.057 1.013 0.972 1.138 1.026 1.042 1.082 0.947 1.033 0.908 0.910 1.032 0.951 1.076 1.058 1.015 1.097 1.012 NOT TAKEN 1.232 1.015 0.968 NOT TAKEN SPILLED 0.921 1.031 SPILLED 1.037 0.920 NOT TAKEN 1.038 1.044 0.991 0.980 1.146 NOT TAKEN 1.038 0.896 0.930 1.025 0.896 0.964 0.999 0.959 0.928 1.055 0.983 NOT TAKEN 0.984 NOT TAKEN 1.201 1.021 1.008 NOT TAKEN SPILLED 1.023 1.011 SPILLED 1.029 0.925 NOT TAKEN 0.985 0.969 0.985 0.952 1.087 NOT TAKEN 1.064 1.048 0.950 1.024 0.900 0.926 1.013 0.991 1.080 1.006 0.963 NOT TAKEN 0.992 NOT TAKEN 1.129 0.988 0.997 NOT TAKEN 1.171 0.981 1.010 1.133 1.079 0.919 NOT TAKEN 0.970 1.045 0.963 0.930 1.145 NOT TAKEN 1.015 SPILLED 0.919 1.004 0.891 0.867 1.001 1.003 1.012 1.015 0.895 NOT TAKEN 0.972 NOT TAKEN 1.097 0.911 0.920 NOT TAKEN SPILLED 1.011 0.962 SPILLED 1.069 0.928 NOT TAKEN 0.993 1.071 1.027 0.887 1.040 NOT TAKEN 1.052 1.012 0.926 1.040 0.882 0.930 0.954 0.989 0.945 1.017 0.933 NOT TAKEN 0.915 NOT TAKEN 1.125 0.898 0.845 NOT TAKEN 1.015 0.914 0.875 1.084 1.051 0.806 NOT TAKEN 0.909 1.016 0.888 0.820 1.020 NOT TAKEN 0.992 0.936 0.822 0.912 0.805 0.867 0.884 0.937 0.884 0.904 0.828 NOT TAKEN 0.829 NOT TAKEN 1.051 0.898 0.789 NOT TAKEN 0.817 0.877 0.811 SPILLED 0.924 0.767 NOT TAKEN 0.914 0.972 0.885 0.805 1.003 NOT TAKEN 0.885 0.849 0.810 0.829 0.837 0.812 0.889 0.978 0.749 0.857 0.819 NOT TAKEN 0.798 NOT TAKEN 1.081 0.817 0.760 NOT TAKEN 0.875 0.870 0.797 0.880 0.902 0.754 NOT TAKEN 0.836 1.013 0.870 0.732 0.952 NOT TAKEN 0.854 0.814 SPILLED 0.857 0.783 0.750 0.854 0.940 0.772 0.828 0.795 NOT TAKEN 0.797 NOT TAKEN 1.026 0.817 1458 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 1.075 0.999 1.115 1.103 1.061 SPILLED 1.055 SPILLED 1.059 1.079 1.027 NOT TAKEN 1.041 1.089 1.000 1.027 NOT TAKEN 1.066 SPILLED 1.025 1.086 1.020 1.119 1.064 1.021 1.060 1.011 1.114 1.079 1.146 0.959 1.054 0.994 1.126 1.040 1.041 1.152 0.983 1.046 1.035 1.062 1.038 NOT TAKEN 0.998 1.044 0.975 1.035 NOT TAKEN 1.028 1.024 0.985 1.051 0.990 1.078 1.020 1.050 1.013 0.945 1.049 1.055 TECH SPILL 0.950 1.023 0.969 1.037 1.052 0.963 SPILLED 0.985 0.905 1.025 1.047 0.994 NOT TAKEN 1.009 0.967 0.949 0.988 NOT TAKEN 1.062 1.013 0.945 1.049 0.984 1.095 1.010 1.020 0.981 0.968 1.118 1.049 1.046 0.932 1.033 0.957 0.943 0.992 0.961 SPILLED 1.027 NOT TAKEN 1.011 1.040 0.959 NOT TAKEN 0.965 0.998 0.958 1.015 NOT TAKEN NOT TAKEN 0.978 0.920 1.025 0.980 1.104 0.971 0.916 0.977 0.958 1.088 1.037 1.083 NOT TAKEN 1.025 0.930 1.051 0.993 1.014 SPILLED 1.000 NOT TAKEN 0.957 1.042 0.980 NOT TAKEN 0.951 1.017 0.993 1.002 NOT TAKEN NOT TAKEN 0.967 0.970 1.041 0.989 1.053 0.964 0.877 1.035 0.972 1.097 1.056 1.100 NOT TAKEN 0.992 0.953 1.041 0.988 1.021 1.073 0.952 NOT TAKEN 0.882 1.064 1.003 NOT TAKEN 0.930 1.031 0.991 1.011 NOT TAKEN NOT TAKEN 0.958 0.913 1.048 0.994 1.049 0.914 0.937 0.986 0.961 1.055 0.975 1.101 NOT TAKEN 0.915 0.898 0.949 0.961 0.972 1.037 0.971 NOT TAKEN 0.923 1.076 0.947 NOT TAKEN 0.880 0.963 0.902 1.054 NOT TAKEN NOT TAKEN 0.958 0.840 0.987 0.973 1.030 0.903 0.884 0.904 0.930 1.024 0.920 1.034 NOT TAKEN 0.886 0.858 0.889 0.865 0.883 0.984 0.867 NOT TAKEN 0.856 0.974 0.876 NOT TAKEN 0.872 0.861 0.909 0.958 NOT TAKEN NOT TAKEN 0.862 0.862 0.913 0.935 0.950 0.825 0.844 0.837 0.872 0.858 0.881 0.944 NOT TAKEN 0.801 0.843 0.897 0.855 0.859 1.045 0.886 NOT TAKEN 0.843 0.973 0.802 NOT TAKEN 0.779 0.840 0.868 0.892 NOT TAKEN NOT TAKEN 0.854 0.830 0.905 0.938 0.897 0.787 0.891 0.802 0.864 0.865 0.848 0.897 NOT TAKEN 0.772 0.790 0.864 0.809 0.828 1.040 0.825 NOT TAKEN 0.821 0.916 0.817 NOT TAKEN 0.797 0.809 0.872 0.884 NOT TAKEN NOT TAKEN 0.797 0.766 0.889 0.931 0.891 0.750 0.810 0.781 0.825 0.809 0.814 0.840 NOT TAKEN 1459 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 1.129 0.989 SPILLED 1.005 1.026 1.069 1.049 SPILLED 1.017 0.983 SPILLED 0.960 0.997 1.010 0.991 1.617 1.045 0.954 SPILLED 0.951 0.990 1.009 1.033 1.563 1.068 1.027 1.246 0.958 0.969 1.010 1.099 1.194 1.021 1.023 SPILLED 0.929 0.964 1.020 1.048 1.407 1.006 1.082 1.256 0.900 0.947 1.035 1.074 1.286 0.965 0.963 SPILLED 0.862 0.934 0.991 1.051 1.115 0.919 0.883 1.108 0.810 0.829 0.886 0.911 0.953 0.904 0.925 1.129 0.833 0.816 0.838 0.896 1.140 0.934 0.857 1.071 0.737 0.792 0.860 0.884 1.138 GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 1.088 1.042 1.053 1.056 1.253 1.116 1.095 1.006 1.068 1.183 1.080 SPILLED 0.995 1.051 1.043 1.116 0.998 1.068 1.086 1.023 1.147 1.031 1.027 1.032 1.074 1.264 1.082 0.975 1.070 1.196 1.085 1.033 0.964 1.023 1.011 0.984 0.962 1.052 1.076 0.998 1.092 0.994 0.985 1.011 1.069 1.145 1.111 1.005 1.057 1.204 1.062 1.021 0.966 0.956 0.994 0.944 0.937 1.070 1.072 1.026 1.023 1.052 0.985 1.037 1.080 1.161 1.183 1.046 1.059 1.179 0.905 1.033 0.993 0.992 1.014 1.005 0.917 1.083 1.022 0.987 1.019 1.021 1.002 0.997 1.060 1.110 1.138 1.008 1.043 1.151 1.070 0.978 0.953 0.989 1.044 0.997 0.954 1.011 1.063 0.992 0.946 0.969 1.015 1.019 SPILLED 1.090 1.105 0.966 1.012 1.090 1.072 0.915 0.927 0.988 1.008 0.927 0.880 0.991 1.046 0.974 0.961 1.013 0.811 0.966 1.056 1.158 1.040 0.991 0.978 0.836 0.959 0.928 0.877 0.978 0.962 0.916 0.960 0.958 0.933 0.886 SPILLED 0.864 0.655 0.885 0.927 1.031 0.982 0.911 0.870 0.953 0.901 0.861 0.811 0.930 0.876 0.826 0.848 0.894 0.884 0.862 1.131 0.874 0.868 SPILLED 0.881 0.970 0.892 0.863 0.842 0.925 0.803 0.809 0.752 0.864 0.845 0.831 0.825 0.883 0.818 0.849 0.937 0.791 0.791 SPILLED SPILLED 0.896 0.859 0.820 0.823 0.887 0.762 0.822 0.732 0.839 0.807 0.805 0.841 0.848 0.798 0.824 1460 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 1.087 1.032 1.051 1.071 1.237 1.099 1.091 1.230 SPILLED 1.183 0.989 1.225 1.029 1.096 1.154 1.073 0.955 1.069 1.064 1.130 0.997 0.961 0.988 0.981 1.167 1.062 1.060 1.183 SPILLED 1.166 0.988 1.143 0.982 1.064 1.062 1.014 0.926 1.012 1.037 1.080 0.768 0.965 0.968 1.003 SPILLED 1.034 1.031 1.222 SPILLED SPILLED 0.964 1.085 0.971 1.023 1.094 1.055 0.933 0.995 0.998 1.059 1.071 0.941 0.920 1.024 1.188 0.953 1.002 SPILLED SPILLED 1.193 0.987 1.023 0.992 1.025 1.484 1.045 0.956 0.963 1.013 1.088 1.054 0.847 0.972 1.043 1.120 0.976 1.006 1.118 1.170 1.162 1.036 1.049 0.948 1.025 1.145 1.038 0.946 0.964 1.008 1.057 0.983 0.936 1.032 1.075 1.120 1.005 0.946 1.071 1.198 SPILLED 1.013 1.099 0.994 1.006 1.067 1.006 0.955 0.923 0.962 1.040 0.948 0.923 1.024 1.082 1.124 0.946 0.944 1.065 SPILLED SPILLED 0.934 1.010 1.007 1.013 0.879 0.947 0.908 0.913 0.997 1.046 0.848 0.864 0.820 0.978 1.004 0.851 0.868 1.048 1.114 1.079 0.874 0.904 0.898 0.932 0.927 0.897 0.780 0.878 0.963 0.960 0.846 0.796 0.835 0.964 0.958 SPILLED 0.808 0.959 SPILLED SPILLED 0.879 0.898 0.876 0.907 0.922 0.877 0.763 0.815 0.883 0.880 0.790 0.788 0.760 0.854 0.921 0.888 0.782 0.891 SPILLED SPILLED 0.981 0.960 0.858 0.811 0.835 0.854 0.728 SPILLED 0.874 0.831 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 0.030 SPILLED 0.036 0.041 0.030 0.029 0.034 0.032 0.028 SPILLED 0.034 SPILLED 0.029 0.032 0.037 SPILLED 0.029 SPILLED 0.031 0.036 0.028 0.030 0.034 0.045 0.026 SPILLED 0.027 SPILLED 0.023 0.028 0.034 SPILLED 0.028 SPILLED 0.029 0.034 0.026 0.030 0.034 0.033 0.028 0.036 0.031 0.034 0.026 0.029 0.033 SPILLED 0.027 SPILLED 0.029 0.032 0.027 0.025 0.034 SPILLED 0.026 0.040 0.027 0.030 0.025 0.024 0.031 SPILLED 0.025 0.041 0.025 0.028 0.021 0.026 0.030 0.025 0.024 0.054 0.024 0.027 0.025 0.024 0.028 SPILLED 1461 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 31 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 0.035 SPILLED NOT TAKEN 0.033 0.029 0.034 NOT TAKEN 0.031 0.034 0.031 0.027 NOT TAKEN 0.029 0.033 0.033 0.034 0.033 0.032 0.035 0.033 0.035 0.031 0.032 0.032 0.033 0.032 0.033 0.032 0.030 0.030 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED 0.031 SPILLED NOT TAKEN 0.031 0.031 0.028 0.030 NOT TAKEN SPILLED 0.030 0.031 0.035 0.030 0.027 0.033 0.032 0.032 0.036 0.030 0.031 0.036 SPILLED 0.034 0.033 SPILLED 0.030 NOT TAKEN SPILLED SPILLED NOT TAKEN 0.034 0.032 SPILLED NOT TAKEN 0.031 0.032 0.031 0.031 NOT TAKEN SPILLED 0.030 0.032 SPILLED 0.030 0.027 SPILLED 0.032 0.035 0.035 0.029 0.031 0.032 0.031 0.031 0.034 0.029 0.031 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED 0.035 NOT TAKEN NOT TAKEN 0.027 0.023 0.025 0.029 NOT TAKEN 0.031 0.033 0.030 0.034 0.029 0.022 SPILLED 0.028 0.034 0.032 0.028 NOT TAKEN 0.034 SPILLED 0.030 0.032 NOT TAKEN 0.028 NOT TAKEN 0.044 0.047 NOT TAKEN 0.033 0.032 NOT TAKEN NOT TAKEN 0.030 0.029 0.030 0.027 NOT TAKEN 0.031 0.031 0.028 0.032 0.029 0.028 0.034 0.031 0.030 0.033 0.028 NOT TAKEN 0.031 0.031 0.031 0.031 NOT TAKEN 0.028 NOT TAKEN SPILLED 0.036 NOT TAKEN SPILLED 0.030 NOT TAKEN NOT TAKEN 0.031 0.030 0.028 0.029 NOT TAKEN 0.032 0.030 0.031 0.032 0.029 0.028 0.033 0.033 0.032 0.032 0.027 NOT TAKEN 0.031 0.050 0.031 0.033 NOT TAKEN 0.028 NOT TAKEN SPILLED 0.033 NOT TAKEN 0.031 0.027 NOT TAKEN NOT TAKEN 0.025 0.030 0.029 0.026 NOT TAKEN 0.033 0.030 0.031 0.030 0.028 0.028 0.032 0.033 0.033 0.032 0.025 NOT TAKEN 0.031 SPILLED 0.030 0.033 NOT TAKEN 0.027 NOT TAKEN SPILLED 0.030 NOT TAKEN 0.029 0.023 NOT TAKEN NOT TAKEN 0.028 0.023 0.029 0.024 NOT TAKEN SPILLED 0.029 0.027 0.027 0.028 0.024 SPILLED 0.029 0.031 SPILLED 0.024 NOT TAKEN 0.027 SPILLED 0.031 0.028 NOT TAKEN 0.025 NOT TAKEN 0.032 0.028 NOT TAKEN 0.029 0.025 NOT TAKEN NOT TAKEN 0.024 0.024 0.025 0.020 NOT TAKEN SPILLED 0.027 0.026 0.029 0.027 0.025 0.029 0.027 0.044 0.035 0.023 NOT TAKEN 0.030 0.029 CNTMD 0.027 NOT TAKEN 0.025 NOT TAKEN SPILLED 0.028 NOT TAKEN 0.028 0.027 NOT TAKEN NOT TAKEN 0.025 0.024 0.023 0.014 NOT TAKEN 0.028 0.025 0.029 0.028 0.024 0.024 0.026 0.028 0.027 0.027 0.021 NOT TAKEN 0.029 0.033 0.031 0.024 NOT TAKEN 0.020 NOT TAKEN 1462 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 32 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0.030 0.031 0.036 0.037 0.032 0.037 0.030 0.035 0.031 0.034 0.034 0.031 NOT TAKEN 0.033 0.031 0.031 0.034 0.030 0.035 SPILLED 0.037 0.032 0.032 0.037 0.036 0.030 0.036 0.030 0.041 0.031 0.029 0.032 SPILLED NOT TAKEN 0.032 0.029 0.031 0.032 0.031 0.031 SPILLED 0.036 0.031 0.030 0.035 SPILLED 0.030 0.035 0.027 0.036 0.029 0.032 0.031 0.030 NOT TAKEN 0.032 0.029 0.032 0.033 0.031 0.035 SPILLED 0.034 0.030 0.032 0.032 0.038 0.034 0.034 0.026 0.033 NOT TAKEN 0.030 0.030 0.030 NOT TAKEN SPILLED 0.027 0.028 0.029 NOT TAKEN 0.036 SPILLED 0.036 0.031 0.028 0.034 0.037 0.032 0.033 0.027 0.031 NOT TAKEN 0.029 0.029 0.029 NOT TAKEN 0.027 0.027 0.028 0.033 NOT TAKEN 0.033 0.038 0.034 0.031 0.030 0.037 0.036 0.031 0.035 0.028 SPILLED NOT TAKEN 0.028 0.028 0.027 NOT TAKEN 0.028 0.026 SPILLED 0.033 NOT TAKEN 0.031 SPILLED 0.033 0.027 0.029 0.033 0.034 0.029 0.035 0.028 0.029 NOT TAKEN 0.025 0.029 0.027 NOT TAKEN 0.035 0.028 0.029 0.031 NOT TAKEN 0.029 0.037 0.030 0.025 0.026 0.030 0.038 0.026 0.030 0.028 0.027 NOT TAKEN 0.028 0.027 0.031 NOT TAKEN 0.026 0.025 0.027 0.028 NOT TAKEN 0.028 0.036 0.030 0.025 0.030 0.028 0.030 0.024 0.030 0.025 0.026 NOT TAKEN 0.026 0.021 0.023 NOT TAKEN 0.026 0.024 0.024 0.027 NOT TAKEN 0.031 0.044 0.031 0.023 0.027 0.028 0.032 0.024 0.027 0.023 0.027 NOT TAKEN 0.023 0.030 0.027 NOT TAKEN 0.026 0.022 0.028 0.026 NOT TAKEN 0.025 SPILLED 0.041 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 0.127 0.137 0.129 0.127 NOT TAKEN NOT TAKEN 0.134 0.123 0.126 0.127 0.134 NOT TAKEN NOT TAKEN 0.128 0.119 0.125 0.120 0.119 NOT TAKEN NOT TAKEN 0.123 0.125 SPILLED NOT TAKEN 0.115 NOT TAKEN NOT TAKEN SPILLED 0.119 0.139 NOT TAKEN 0.131 NOT TAKEN NOT TAKEN 0.127 0.126 0.134 NOT TAKEN 0.122 NOT TAKEN NOT TAKEN SPILLED 0.123 0.133 NOT TAKEN 0.122 NOT TAKEN NOT TAKEN SPILLED 0.115 0.128 NOT TAKEN 0.106 NOT TAKEN NOT TAKEN 0.124 0.129 0.127 NOT TAKEN 0.106 NOT TAKEN NOT TAKEN 0.129 0.120 0.126 NOT TAKEN 0.102 NOT TAKEN NOT TAKEN 0.120 1463 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 33 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 0.143 SPILLED 0.129 0.141 0.137 0.109 0.132 0.133 NOT TAKEN 0.132 NOT TAKEN SPILLED 0.152 0.125 0.132 0.138 0.123 0.128 0.128 0.152 0.135 0.132 0.132 0.132 0.125 SPILLED 0.144 0.147 0.142 0.127 0.139 0.139 SPILLED 0.148 0.146 0.143 0.123 0.133 0.133 NOT TAKEN SPILLED NOT TAKEN 0.171 0.147 0.117 0.120 0.147 0.140 SPILLED SPILLED 0.148 0.127 SPILLED 0.196 0.114 0.112 SPILLED 0.141 0.146 0.137 0.136 0.128 0.135 0.134 0.123 0.133 0.130 0.118 0.125 0.138 NOT TAKEN 0.115 NOT TAKEN 0.128 0.139 0.110 0.121 0.124 0.122 SPILLED 0.143 0.147 0.123 SPILLED 0.134 0.119 0.112 SPILLED 0.128 0.137 0.122 0.128 SPILLED 0.136 SPILLED NOT TAKEN 0.129 0.133 0.114 0.123 0.146 NOT TAKEN 0.129 NOT TAKEN 0.139 SPILLED 0.115 0.122 0.145 0.116 SPILLED 0.142 0.149 NOT TAKEN SPILLED 0.146 0.121 0.119 NOT TAKEN 0.128 0.132 0.108 0.154 SPILLED 0.135 0.150 NOT TAKEN 0.132 0.118 0.121 0.126 0.141 NOT TAKEN 0.132 NOT TAKEN 0.122 0.145 0.113 0.128 0.124 0.124 0.128 SPILLED 0.137 NOT TAKEN SPILLED 0.132 0.108 0.106 NOT TAKEN 0.143 0.141 0.120 0.118 SPILLED 0.139 SPILLED NOT TAKEN 0.131 0.133 0.111 0.120 0.128 NOT TAKEN 0.118 NOT TAKEN 0.123 0.169 0.118 0.124 0.133 0.112 SPILLED 0.171 0.135 NOT TAKEN 0.127 0.126 0.117 0.112 NOT TAKEN 0.129 0.104 0.120 0.120 0.131 0.123 SPILLED NOT TAKEN 0.139 0.131 0.127 0.112 0.149 NOT TAKEN 0.116 NOT TAKEN 0.117 0.130 0.103 0.121 0.115 0.113 SPILLED SPILLED 0.128 NOT TAKEN 0.123 0.128 0.128 0.103 NOT TAKEN 0.119 0.129 0.127 0.115 SPILLED 0.124 0.109 NOT TAKEN 0.145 0.119 0.108 0.105 0.133 NOT TAKEN 0.114 NOT TAKEN 0.122 0.117 0.099 0.114 0.142 0.093 SPILLED SPILLED 0.112 NOT TAKEN SPILLED 0.132 0.110 0.099 NOT TAKEN 0.118 0.109 0.124 0.114 0.120 0.125 0.117 NOT TAKEN 0.142 0.124 0.123 0.094 0.116 NOT TAKEN 0.112 NOT TAKEN 0.139 0.110 0.096 0.114 0.108 0.107 0.111 0.206 0.120 NOT TAKEN 0.110 0.122 0.102 0.092 NOT TAKEN 0.118 0.119 0.118 0.108 SPILLED 0.106 0.112 NOT TAKEN 0.121 0.120 0.094 0.101 0.116 NOT TAKEN 0.101 NOT TAKEN 0.116 0.124 0.105 0.106 0.110 0.105 0.122 0.167 0.114 NOT TAKEN 0.105 0.096 0.098 0.099 NOT TAKEN 0.110 0.103 0.120 0.104 SPILLED 1464 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 34 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 SPILLED 0.146 NOT TAKEN 0.131 SPILLED 0.147 0.142 SPILLED 0.133 SPILLED 0.146 SPILLED 0.165 0.133 0.145 0.140 NOT TAKEN 0.107 0.134 0.154 0.137 0.145 SPILLED SPILLED NOT TAKEN 0.138 SPILLED SPILLED 0.134 0.131 0.134 SPILLED 0.134 SPILLED SPILLED 0.132 0.150 0.126 NOT TAKEN 0.156 0.128 SPILLED 0.133 SPILLED SPILLED 0.131 NOT TAKEN 0.128 SPILLED 0.133 0.125 0.121 0.127 0.149 0.134 SPILLED 0.158 0.125 0.142 0.122 NOT TAKEN 0.135 0.117 0.155 0.131 0.151 SPILLED 0.119 NOT TAKEN 0.117 SPILLED 0.123 NOT TAKEN SPILLED 0.128 0.148 0.127 0.151 SPILLED 0.133 0.132 0.129 NOT TAKEN 0.123 0.121 0.144 0.129 SPILLED SPILLED 0.142 NOT TAKEN 0.124 SPILLED 0.119 NOT TAKEN 0.127 0.124 0.149 0.119 SPILLED 0.137 0.115 0.142 0.130 NOT TAKEN 0.125 0.124 0.144 0.126 0.140 SPILLED SPILLED NOT TAKEN 0.130 SPILLED 0.127 NOT TAKEN 0.109 0.146 0.153 0.112 SPILLED 0.143 0.101 0.131 SPILLED NOT TAKEN 0.145 0.117 0.162 0.129 0.154 SPILLED 0.125 NOT TAKEN 0.127 SPILLED 0.107 NOT TAKEN 0.117 0.104 0.135 0.112 0.131 0.148 0.143 0.119 0.122 NOT TAKEN 0.124 0.106 0.144 0.122 0.137 SPILLED 0.121 NOT TAKEN 0.119 0.159 0.117 NOT TAKEN 0.116 0.101 0.132 0.109 0.155 0.147 0.126 0.129 SPILLED NOT TAKEN 0.126 0.093 0.137 0.112 0.138 0.165 0.104 NOT TAKEN 0.116 SPILLED 0.116 NOT TAKEN 0.103 0.108 0.121 0.108 0.161 0.129 0.122 0.111 0.178 NOT TAKEN TECH SPILL 0.099 SPILLED 0.114 0.126 SPILLED 0.111 NOT TAKEN 0.110 0.121 0.113 NOT TAKEN 0.090 0.100 0.121 0.100 0.142 0.115 0.105 0.115 SPILLED NOT TAKEN SPILLED 0.107 0.125 0.108 0.124 GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 0.351 SPILLED 0.385 SPILLED SPILLED 0.321 0.397 SPILLED 0.345 SPILLED 0.471 0.322 0.358 SPILLED 0.332 0.358 0.328 0.321 NOT TAKEN SPILLED 0.357 0.379 SPILLED 0.305 NOT TAKEN SPILLED 0.330 0.337 0.312 0.311 NOT TAKEN SPILLED 0.366 SPILLED 0.307 0.311 NOT TAKEN SPILLED 0.326 0.331 0.300 0.322 NOT TAKEN SPILLED 0.301 0.325 0.268 0.293 NOT TAKEN 0.282 0.272 0.320 0.286 0.314 NOT TAKEN SPILLED 0.262 0.307 0.296 0.299 1465 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 35 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 0.366 NOT TAKEN 0.348 0.360 0.348 0.347 0.319 0.372 0.380 0.367 NOT TAKEN 0.336 0.313 NOT TAKEN 0.340 0.420 0.371 0.431 NOT TAKEN SPILLED 0.324 0.360 0.345 0.343 0.323 0.295 0.343 NOT TAKEN 0.345 0.314 0.362 0.359 NOT TAKEN 0.346 0.345 0.296 0.327 0.293 0.340 0.417 0.333 NOT TAKEN 0.338 0.307 NOT TAKEN 0.329 0.413 0.365 SPILLED NOT TAKEN 0.322 0.300 0.455 0.361 0.335 0.329 0.295 0.342 NOT TAKEN 0.338 0.373 0.364 0.362 NOT TAKEN 0.320 0.313 0.314 0.319 0.324 0.331 0.360 0.326 NOT TAKEN 0.315 0.306 NOT TAKEN 0.316 SPILLED 0.363 0.361 NOT TAKEN SPILLED 0.288 SPILLED 0.351 0.317 0.332 0.294 0.349 NOT TAKEN SPILLED 0.303 0.303 0.351 NOT TAKEN 0.325 0.319 0.298 0.310 0.297 NOT TAKEN 0.368 0.321 NOT TAKEN 0.314 0.289 NOT TAKEN NOT TAKEN 0.348 0.336 SPILLED NOT TAKEN 0.260 0.270 SPILLED 0.330 0.296 0.303 0.285 0.336 NOT TAKEN NOT TAKEN SPILLED 0.314 0.353 NOT TAKEN 0.315 0.319 0.319 0.314 0.306 NOT TAKEN 0.356 0.330 NOT TAKEN 0.309 0.278 NOT TAKEN NOT TAKEN SPILLED 0.349 0.351 NOT TAKEN SPILLED 0.262 0.353 0.366 0.320 0.319 0.291 0.346 NOT TAKEN NOT TAKEN 0.309 0.313 0.325 NOT TAKEN 0.338 0.296 0.304 0.305 0.290 NOT TAKEN 0.367 0.317 NOT TAKEN 0.310 0.280 NOT TAKEN NOT TAKEN 0.349 0.357 0.369 NOT TAKEN SPILLED 0.266 0.366 0.316 0.293 0.288 0.304 0.334 NOT TAKEN NOT TAKEN 0.306 0.326 0.312 NOT TAKEN 0.307 0.277 0.321 0.308 0.265 NOT TAKEN 0.340 SPILLED NOT TAKEN 0.294 0.286 NOT TAKEN NOT TAKEN 0.348 0.335 0.357 NOT TAKEN 0.306 0.299 0.401 0.311 0.384 0.304 0.305 0.305 NOT TAKEN NOT TAKEN 0.305 0.317 0.322 NOT TAKEN 0.278 0.279 0.312 0.293 0.251 NOT TAKEN 0.323 SPILLED NOT TAKEN 0.296 0.298 NOT TAKEN NOT TAKEN 0.322 0.316 0.303 NOT TAKEN 0.287 0.277 0.306 0.264 0.311 0.277 0.316 0.294 NOT TAKEN NOT TAKEN 0.268 0.283 0.304 NOT TAKEN 0.267 0.249 0.296 0.298 0.251 NOT TAKEN 0.304 0.335 NOT TAKEN 0.250 0.251 NOT TAKEN NOT TAKEN 0.318 0.306 0.272 NOT TAKEN 0.273 0.263 0.309 0.277 SPILLED 0.304 0.265 0.294 NOT TAKEN NOT TAKEN 0.271 0.273 0.304 NOT TAKEN 0.280 0.270 0.278 SPILLED 0.260 NOT TAKEN 0.272 SPILLED NOT TAKEN 0.284 0.261 NOT TAKEN NOT TAKEN 0.293 0.288 0.242 NOT TAKEN 0.235 0.252 0.310 0.253 SPILLED 0.266 0.261 0.267 NOT TAKEN NOT TAKEN 0.248 0.297 1466 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 36 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.300 0.355 0.345 0.303 0.365 0.293 0.345 0.337 0.345 SPILLED 0.332 0.322 0.399 0.326 SPILLED 0.346 SPILLED 0.337 0.338 0.325 0.324 0.351 0.379 0.359 0.317 0.311 0.308 0.350 0.292 0.349 SPILLED 0.361 SPILLED 0.351 0.289 0.374 0.300 SPILLED 0.324 0.404 0.346 0.307 0.300 SPILLED 0.334 0.362 0.326 0.296 0.316 0.288 0.307 0.335 0.310 0.349 0.343 SPILLED 0.309 0.301 0.381 0.267 0.329 0.335 0.355 0.318 0.298 0.275 0.298 0.291 0.366 0.335 0.268 0.284 0.298 0.322 0.237 0.304 0.316 SPILLED SPILLED 0.349 0.283 0.346 0.290 SPILLED 0.302 SPILLED 0.290 0.292 0.283 0.282 NOT TAKEN SPILLED 0.323 0.314 0.334 0.286 0.329 0.322 0.319 0.317 0.323 SPILLED 0.297 0.280 0.353 0.300 0.335 0.286 SPILLED 0.299 0.313 0.289 0.298 NOT TAKEN 0.326 0.340 0.295 0.298 0.267 0.320 0.331 0.334 0.316 0.311 SPILLED 0.308 0.291 0.369 0.285 0.331 0.290 0.322 0.299 0.295 0.286 0.300 NOT TAKEN SPILLED 0.327 0.310 0.292 0.295 0.366 SPILLED 0.313 0.302 0.320 SPILLED 0.312 0.260 0.376 SPILLED 0.347 0.318 0.305 0.298 0.310 0.285 SPILLED NOT TAKEN 0.291 0.300 0.280 0.262 0.278 0.315 SPILLED 0.267 0.255 0.296 0.329 0.286 0.292 0.316 0.257 0.302 0.212 0.285 0.261 0.289 0.250 0.275 NOT TAKEN 0.288 0.314 0.265 0.262 0.281 0.311 0.220 0.276 0.251 0.250 0.304 0.254 0.277 0.284 0.264 SPILLED 0.288 0.255 0.248 0.294 0.253 0.244 NOT TAKEN 0.282 0.293 0.279 0.247 0.231 0.325 0.265 0.275 0.242 NOT TAKEN 0.289 0.266 0.270 0.290 0.253 0.302 0.372 0.243 0.252 0.272 0.254 0.242 NOT TAKEN 0.223 GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 1.235 1.298 1.511 NOT TAKEN 1.445 1.190 1.219 1.411 NOT TAKEN 1.486 1.065 1.118 1.276 NOT TAKEN 1.406 NOT TAKEN 1.208 1.315 NOT TAKEN 1.395 NOT TAKEN 1.149 1.498 NOT TAKEN 1.331 NOT TAKEN 1.197 1.420 NOT TAKEN 1.346 NOT TAKEN SPILLED 1.347 NOT TAKEN 1.323 NOT TAKEN 1.114 1.249 NOT TAKEN 1.271 NOT TAKEN 1.123 1.191 NOT TAKEN 1.150 NOT TAKEN 1.093 1.231 NOT TAKEN 1.087 1467 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 37 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 SPILLED 1.326 1.416 1.455 1.276 1.321 1.263 NOT TAKEN 1.451 1.533 1.356 1.366 1.374 1.481 1.434 1.338 1.347 1.369 1.240 1.349 1.420 1.453 1.175 1.575 1.538 1.448 NOT TAKEN SPILLED NOT TAKEN 1.673 1.370 1.405 1.374 1.373 1.442 1.491 1.386 1.280 NOT TAKEN 1.404 1.581 1.224 1.429 1.454 1.382 1.493 1.202 1.257 1.385 1.238 1.381 1.434 1.497 1.310 1.497 1.473 1.389 NOT TAKEN 1.824 NOT TAKEN 1.488 1.363 1.259 1.173 1.381 1.351 1.370 1.286 0.968 NOT TAKEN 1.441 1.562 1.182 1.252 1.385 1.336 1.332 1.202 1.192 1.246 1.131 1.366 1.298 1.443 1.223 1.471 1.321 1.434 NOT TAKEN 1.813 NOT TAKEN SPILLED 1.373 1.232 1.257 1.417 1.317 1.204 1.257 1.202 NOT TAKEN 1.086 1.579 1.277 NOT TAKEN 1.316 1.394 1.321 1.196 1.256 1.420 1.199 1.195 1.377 1.513 1.193 1.483 1.371 1.287 NOT TAKEN NOT TAKEN NOT TAKEN 1.593 SPILLED 1.311 SPILLED 1.417 1.378 1.443 1.273 1.199 NOT TAKEN 1.113 1.469 1.292 NOT TAKEN 1.295 1.283 1.382 1.172 1.287 1.386 1.176 1.452 1.410 1.483 1.194 1.429 1.470 1.342 NOT TAKEN NOT TAKEN NOT TAKEN 1.750 1.224 1.282 1.258 1.498 1.266 1.257 1.261 1.197 NOT TAKEN 1.277 1.441 1.284 NOT TAKEN 1.306 1.466 1.367 SPILLED 1.286 1.457 1.156 1.299 1.405 1.409 1.213 1.378 1.464 1.491 NOT TAKEN NOT TAKEN NOT TAKEN 1.755 1.316 1.265 SPILLED 1.401 1.303 1.355 1.319 1.185 NOT TAKEN 1.218 1.499 1.315 NOT TAKEN 1.265 1.544 1.317 1.168 1.385 SPILLED 1.220 1.168 1.410 1.467 1.234 SPILLED 1.376 1.301 NOT TAKEN NOT TAKEN NOT TAKEN 1.423 1.458 1.280 1.190 1.258 1.190 1.291 1.215 1.076 NOT TAKEN 1.279 1.234 1.219 NOT TAKEN 1.153 1.604 1.208 1.157 1.178 SPILLED 1.054 1.049 1.419 1.301 1.143 1.235 1.233 1.168 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.165 1.177 1.232 0.955 1.228 1.229 1.134 1.152 NOT TAKEN 1.055 1.232 1.223 NOT TAKEN 1.039 1.177 1.022 1.121 1.109 SPILLED 1.058 1.154 1.324 1.224 1.106 1.234 1.091 1.214 NOT TAKEN NOT TAKEN NOT TAKEN 1.778 0.993 1.231 1.209 1.197 1.202 1.196 1.101 1.180 NOT TAKEN 0.985 1.198 1.189 NOT TAKEN 1.219 1.270 1.212 1.095 1.068 1.200 0.982 1.312 1.317 1.185 1.001 1.242 1.135 1.078 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.129 1468 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 38 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 1.237 1.399 1.392 SPILLED 1.336 1.331 SPILLED 1.303 SPILLED 1.323 1.487 1.312 NOT TAKEN 1.327 1.352 SPILLED 1.269 1.332 1.233 1.214 1.285 1.257 1.408 SPILLED SPILLED 1.289 1.309 SPILLED 1.294 1.294 SPILLED 1.380 1.332 SPILLED 1.611 1.319 1.366 2.495 1.266 SPILLED 1.267 1.519 1.305 NOT TAKEN 1.445 1.293 SPILLED 1.293 1.372 1.313 1.282 1.276 SPILLED 1.380 1.834 SPILLED 1.314 1.288 SPILLED 1.417 1.304 SPILLED 1.297 1.385 SPILLED 1.453 1.260 1.299 SPILLED 1.299 2.449 1.249 1.329 1.330 NOT TAKEN 1.346 SPILLED 1.266 1.269 1.325 1.221 1.163 1.233 1.340 1.310 1.326 1.218 1.389 SPILLED SPILLED 1.233 1.281 1.315 1.277 1.239 1.614 1.275 1.252 1.328 SPILLED 1.224 NOT TAKEN 1.205 1.299 1.302 NOT TAKEN 1.317 1.183 1.030 1.245 1.234 1.283 1.087 1.252 1.383 NOT TAKEN SPILLED SPILLED SPILLED 1.173 SPILLED 1.281 1.271 1.256 1.308 1.285 1.303 1.506 1.285 1.349 SPILLED 1.270 NOT TAKEN 1.228 1.350 1.270 NOT TAKEN 1.311 1.368 1.211 1.223 1.328 1.256 1.255 1.224 1.343 NOT TAKEN 1.714 1.400 1.257 1.228 SPILLED 1.248 1.201 1.336 1.301 1.277 1.269 1.313 1.243 1.266 SPILLED 1.241 NOT TAKEN 1.211 1.322 1.318 NOT TAKEN 1.264 1.212 SPILLED 1.200 1.375 1.235 1.100 1.214 1.414 NOT TAKEN 1.516 1.222 1.245 1.227 SPILLED 1.243 1.237 1.301 1.291 1.278 1.273 1.398 1.334 SPILLED SPILLED 1.184 NOT TAKEN 1.194 1.383 1.337 NOT TAKEN 1.302 1.227 SPILLED 1.573 1.262 1.195 1.195 1.121 SPILLED NOT TAKEN SPILLED 1.143 1.278 1.156 SPILLED 1.235 1.275 SPILLED 1.213 1.261 1.125 1.319 1.274 SPILLED 1.312 1.106 NOT TAKEN 1.142 1.212 1.278 NOT TAKEN 1.136 1.117 SPILLED 1.375 1.247 1.120 1.179 1.118 1.078 NOT TAKEN SPILLED 1.087 1.324 1.352 SPILLED 1.183 1.182 1.573 1.125 1.150 1.098 1.320 1.161 SPILLED 1.438 1.081 NOT TAKEN 1.120 1.291 1.227 NOT TAKEN 1.336 1.113 1.162 1.088 1.175 1.081 SPILLED 1.030 1.141 NOT TAKEN 1.992 1.117 1.218 1.133 SPILLED 1.064 1.104 1.191 1.107 1.092 1.133 1.236 1.130 1.231 1.176 1.141 NOT TAKEN 1.142 1.188 1.193 NOT TAKEN 1.308 1.087 1.196 1.068 1.164 1.092 0.977 0.946 1.149 NOT TAKEN 1.671 1.151 1.067 0.998 SPILLED 1.104 1.028 1.146 1469 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 39 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93178 C93179 C93180 1.304 1.485 1.218 1.306 1.506 1.288 1.347 1.571 1.285 1.161 1.274 1.309 1.412 1.328 1.400 1.309 1.517 1.280 1.344 1.352 1.372 1.162 1.172 1.203 1.096 1.219 1.115 1.031 1.221 1.094 GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 1.431 1.220 1.248 1.383 1.339 1.359 1.469 1.293 1.443 1.396 1.165 1.471 1.392 1.523 1.257 1.488 1.317 1.294 1.324 SPILLED 1.338 1.257 1.325 1.451 1.672 1.412 1.254 1.250 1.365 1.509 1.322 1.354 1.271 1.393 1.399 1.172 1.492 1.384 1.556 1.229 1.366 1.438 1.453 1.320 SPILLED 1.629 1.324 1.321 1.384 1.297 1.467 1.355 1.264 1.345 SPILLED 1.336 1.362 1.311 1.355 1.293 1.168 1.358 1.379 1.536 1.224 1.429 1.316 1.415 1.292 SPILLED 1.497 SPILLED 1.204 1.395 1.249 1.347 1.236 1.210 1.331 1.416 1.501 1.309 1.222 1.556 1.322 1.183 1.321 1.429 SPILLED 1.197 1.510 1.347 1.313 1.186 SPILLED 1.364 SPILLED 1.403 1.415 1.286 1.346 1.253 1.261 1.361 1.322 1.366 1.383 1.227 1.304 1.338 1.156 1.388 1.302 1.447 1.196 1.411 1.322 1.286 1.199 2.005 1.417 1.202 1.490 1.328 1.319 1.308 1.143 1.159 1.372 1.459 1.379 SPILLED 1.343 1.417 1.255 1.206 1.385 1.376 1.612 1.141 1.417 1.213 1.277 1.201 SPILLED SPILLED 1.669 1.324 1.324 1.177 1.423 1.318 1.187 1.415 1.379 1.485 1.543 1.347 1.416 1.270 1.231 SPILLED 1.409 SPILLED SPILLED 1.376 SPILLED 1.328 1.236 1.592 SPILLED 1.272 1.528 1.322 1.239 1.284 1.105 1.095 1.374 1.373 1.225 1.271 1.248 1.275 1.215 1.178 1.201 1.339 1.051 1.102 1.380 SPILLED 1.151 1.124 1.381 SPILLED 1.133 1.213 1.200 1.084 1.221 1.056 1.046 1.268 0.656 1.128 1.154 1.197 1.191 1.186 1.067 1.214 1.142 1.072 1.138 1.184 1.088 1.105 1.094 1.189 1.790 1.058 1.212 1.156 1.029 1.181 1.076 0.939 1.202 1.350 1.295 1.032 1.085 1.252 1.243 1.131 1.241 1.110 1.131 1.103 1.206 1.159 1.151 1.114 1.194 1.665 1.074 1.126 1.147 1.049 1470 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 40 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 11 12 13 14 15 16 20 24 28 32 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 1.571 1.468 1.440 SPILLED 1.306 1.324 1.600 1.423 1.423 1.291 1.289 1.399 1.666 1.298 1.566 SPILLED 1.597 1.342 SPILLED 1.341 1.374 1.381 1.232 1.365 1.284 1.236 SPILLED 1.621 1.028 SPILLED 1.345 1.458 1.174 SPILLED 1.439 1.297 1.220 1.353 1.323 1.213 1.210 SPILLED 1.614 1.433 1.804 SPILLED 1.307 1.228 SPILLED 1.272 1.342 1.266 1.266 1.364 1.224 1.158 SPILLED SPILLED 1.191 SPILLED 1.461 1.313 1.229 1.308 1.207 1.222 1.343 1.143 1.309 1.200 1.232 SPILLED 1.549 1.135 1.718 SPILLED 1.290 1.186 SPILLED 1.320 1.302 1.325 1.124 1.331 1.155 1.124 1.328 1.466 1.148 SPILLED SPILLED 1.361 1.179 SPILLED SPILLED 1.323 1.338 SPILLED 1.350 1.279 1.258 SPILLED SPILLED 1.254 SPILLED SPILLED 1.194 1.058 SPILLED 1.224 1.138 1.113 SPILLED 1.262 1.116 1.202 SPILLED SPILLED 1.090 SPILLED 1.285 1.190 1.095 1.702 1.204 1.119 1.083 1.015 1.187 1.040 1.158 1.098 1.342 1.027 1.072 1.221 1.257 1.033 1.795 1.170 1.112 1.150 1.022 1.132 0.995 1.163 1.104 1.106 1.150 1.127 1471 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 41 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 0.019 0.020 0.016 NOT TAKEN NOT TAKEN 0.019 0.018 0.018 0.018 0.018 0.018 0.019 0.019 0.023 0.004 0.019 0.019 0.019 0.018 0.018 0.018 0.018 NOT TAKEN 0.020 0.019 0.019 0.021 0.020 0.020 NOT TAKEN 0.020 0.019 0.020 0.014 NOT TAKEN NOT TAKEN 0.018 0.016 0.018 0.017 0.018 0.017 0.018 0.019 0.021 NOT TAKEN 0.018 0.017 0.018 0.018 0.018 0.018 0.017 NOT TAKEN 0.019 0.018 0.018 0.022 0.020 0.020 NOT TAKEN 0.019 0.018 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.016 0.017 0.017 0.019 0.017 0.019 0.019 0.024 NOT TAKEN 0.017 0.018 0.019 0.018 0.018 0.017 0.018 NOT TAKEN 0.019 0.018 0.019 0.022 0.020 0.020 NOT TAKEN 0.019 0.018 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.015 0.017 0.016 0.018 0.017 0.020 0.018 0.024 NOT TAKEN 0.016 0.017 0.018 0.017 0.017 0.018 0.016 NOT TAKEN 0.019 0.017 0.019 0.022 0.019 0.018 NOT TAKEN 0.018 0.017 0.016 0.015 NOT TAKEN NOT TAKEN 0.017 0.016 0.016 0.017 0.017 0.017 0.018 0.018 SPILLED NOT TAKEN 0.015 0.016 0.017 0.017 0.017 0.017 0.016 NOT TAKEN 0.018 0.018 0.018 0.020 0.018 0.017 NOT TAKEN 0.018 0.017 0.019 0.016 NOT TAKEN NOT TAKEN 0.018 0.016 0.017 0.017 0.018 0.018 0.018 0.018 SPILLED NOT TAKEN 0.016 0.018 0.019 0.018 0.017 0.017 0.018 NOT TAKEN 0.019 0.017 0.018 0.020 0.018 NOT TAKEN NOT TAKEN 0.018 0.017 0.019 0.015 NOT TAKEN NOT TAKEN 0.017 0.016 0.017 0.018 0.018 0.018 0.019 0.018 NOT TAKEN NOT TAKEN 0.015 0.018 0.019 0.018 0.017 0.016 0.017 NOT TAKEN 0.020 0.017 0.018 0.019 0.018 NOT TAKEN NOT TAKEN 0.017 0.016 SPILLED 0.015 NOT TAKEN NOT TAKEN 0.017 0.015 0.016 0.017 0.017 0.017 0.018 0.018 NOT TAKEN NOT TAKEN 0.016 0.017 0.019 0.017 0.017 0.017 0.016 NOT TAKEN 0.018 0.018 0.017 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.017 0.018 0.010 NOT TAKEN NOT TAKEN 0.017 0.015 0.016 0.016 0.019 0.018 SPILLED 0.017 NOT TAKEN NOT TAKEN 0.005 0.018 0.019 0.018 0.018 0.017 0.017 NOT TAKEN 0.020 0.016 0.005 0.019 0.017 NOT TAKEN NOT TAKEN 0.018 0.014 0.017 0.016 NOT TAKEN NOT TAKEN 0.016 0.015 0.016 0.014 0.013 0.018 0.017 0.018 NOT TAKEN NOT TAKEN 0.000 0.017 0.018 0.017 0.017 0.016 0.016 NOT TAKEN 0.018 0.017 NOT TAKEN 0.019 0.017 NOT TAKEN NOT TAKEN 0.017 1472 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 42 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 0.022 NOT TAKEN 0.018 0.017 0.019 0.021 0.016 0.020 NOT TAKEN 0.018 0.019 NOT TAKEN SPILLED 0.018 NOT TAKEN 0.018 NOT TAKEN 0.018 0.020 NOT TAKEN 0.019 0.018 0.019 0.018 0.016 NOT TAKEN 0.022 0.019 0.021 0.020 NOT TAKEN 0.018 0.017 0.019 0.021 0.019 0.020 NOT TAKEN 0.018 0.017 NOT TAKEN SPILLED 0.018 NOT TAKEN 0.018 NOT TAKEN 0.019 0.020 NOT TAKEN 0.017 0.017 0.019 0.017 0.021 NOT TAKEN SPILLED 0.018 0.021 0.021 NOT TAKEN 0.017 0.015 0.018 0.019 0.017 0.020 NOT TAKEN 0.017 0.017 NOT TAKEN 0.025 0.016 NOT TAKEN 0.017 NOT TAKEN 0.020 0.019 NOT TAKEN 0.017 0.016 0.017 0.017 0.018 NOT TAKEN 0.023 0.018 0.020 0.021 NOT TAKEN 0.017 0.015 0.017 0.019 0.019 0.018 NOT TAKEN 0.018 0.016 NOT TAKEN 0.024 0.017 NOT TAKEN 0.016 NOT TAKEN 0.020 0.020 NOT TAKEN 0.017 0.017 0.018 0.017 0.020 NOT TAKEN 0.023 0.017 0.020 0.021 NOT TAKEN 0.017 0.015 0.017 0.017 0.019 0.018 NOT TAKEN 0.018 0.016 NOT TAKEN SPILLED 0.016 NOT TAKEN 0.016 NOT TAKEN 0.018 0.021 NOT TAKEN 0.016 0.017 0.018 0.015 0.020 NOT TAKEN 0.012 0.016 0.019 0.021 NOT TAKEN 0.017 0.017 0.019 0.019 0.018 0.019 NOT TAKEN 0.018 0.017 NOT TAKEN SPILLED 0.016 NOT TAKEN 0.019 NOT TAKEN SPILLED 0.020 NOT TAKEN 0.017 0.017 0.018 0.017 0.017 NOT TAKEN SPILLED 0.017 0.020 0.023 NOT TAKEN 0.017 0.016 0.018 0.021 0.017 0.018 NOT TAKEN 0.017 0.016 NOT TAKEN 0.026 0.016 NOT TAKEN 0.020 NOT TAKEN 0.019 0.019 NOT TAKEN 0.017 0.016 0.019 0.017 0.017 NOT TAKEN 0.006 0.017 0.019 0.023 NOT TAKEN 0.016 0.016 0.018 0.019 0.016 0.018 NOT TAKEN 0.018 0.016 NOT TAKEN SPILLED 0.014 NOT TAKEN 0.019 NOT TAKEN 0.019 0.020 NOT TAKEN 0.017 0.017 NOT TAKEN 0.016 0.020 NOT TAKEN SPILLED 0.016 0.020 0.024 NOT TAKEN 0.015 0.015 0.018 0.019 0.017 0.018 NOT TAKEN 0.018 0.015 NOT TAKEN SPILLED 0.016 NOT TAKEN 0.022 NOT TAKEN 0.020 0.021 NOT TAKEN 0.017 0.017 NOT TAKEN 0.016 0.021 NOT TAKEN 0.015 0.015 0.019 0.023 NOT TAKEN 0.012 0.016 0.019 0.019 0.009 0.018 NOT TAKEN 0.017 0.013 NOT TAKEN SPILLED 0.015 NOT TAKEN 0.021 NOT TAKEN 0.019 0.019 NOT TAKEN 0.016 0.016 NOT TAKEN 0.016 0.014 NOT TAKEN 0.016 0.012 0.020 1473 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 43 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 0.081 0.071 0.072 0.077 0.077 0.072 0.085 0.070 0.073 NOT TAKEN 0.075 0.066 0.082 0.083 NOT TAKEN NOT TAKEN 0.072 0.078 0.097 NOT TAKEN 0.075 NOT TAKEN 0.073 0.079 0.077 0.069 0.077 0.075 0.076 0.099 0.067 0.078 0.065 0.077 0.075 0.075 0.071 0.084 0.070 0.072 NOT TAKEN 0.084 0.064 0.081 0.081 NOT TAKEN NOT TAKEN 0.074 0.075 0.090 NOT TAKEN 0.074 NOT TAKEN 0.071 0.077 0.082 0.069 0.072 0.073 0.077 SPILLED 0.076 0.077 0.064 0.068 0.077 0.074 0.075 0.079 0.064 0.071 NOT TAKEN 0.076 0.064 0.080 0.077 NOT TAKEN NOT TAKEN 0.073 0.076 0.093 NOT TAKEN 0.074 NOT TAKEN 0.070 0.073 0.076 0.067 0.072 0.073 0.079 0.099 0.074 0.075 0.065 0.070 0.074 0.069 0.078 0.080 0.068 0.069 NOT TAKEN 0.075 0.065 0.086 0.079 NOT TAKEN NOT TAKEN 0.070 0.070 SPILLED NOT TAKEN 0.075 NOT TAKEN 0.064 0.070 0.075 0.063 0.070 0.071 0.076 0.103 0.072 0.071 0.063 0.064 0.071 0.071 0.081 0.078 0.064 0.067 NOT TAKEN 0.084 0.066 0.083 0.077 NOT TAKEN NOT TAKEN 0.067 0.069 0.087 NOT TAKEN 0.077 NOT TAKEN 0.069 0.072 0.075 0.064 0.071 0.067 0.077 SPILLED 0.073 0.075 0.064 0.068 0.078 0.073 0.082 0.076 0.063 0.070 NOT TAKEN 0.073 0.065 SPILLED 0.081 NOT TAKEN NOT TAKEN 0.071 0.075 0.100 NOT TAKEN 0.077 NOT TAKEN 0.062 0.077 SPILLED 0.068 0.075 0.073 0.081 SPILLED 0.074 0.073 0.057 0.069 0.075 0.073 SPILLED 0.081 0.066 0.072 NOT TAKEN 0.047 0.066 0.090 0.081 NOT TAKEN NOT TAKEN 0.068 0.072 0.089 NOT TAKEN 0.078 NOT TAKEN 0.074 0.080 0.073 0.066 0.070 0.075 0.081 SPILLED 0.075 0.073 0.052 0.068 0.071 0.064 SPILLED 0.077 0.063 0.070 NOT TAKEN 0.073 0.060 SPILLED 0.077 NOT TAKEN NOT TAKEN 0.070 0.072 0.078 NOT TAKEN 0.067 NOT TAKEN 0.051 0.086 0.070 0.067 0.072 0.069 0.079 0.098 0.081 0.075 0.066 0.070 0.070 0.073 SPILLED 0.079 0.065 0.066 NOT TAKEN 0.081 0.065 SPILLED 0.077 NOT TAKEN NOT TAKEN 0.071 0.069 0.091 NOT TAKEN 0.066 NOT TAKEN 0.070 0.075 0.078 0.066 0.072 0.070 0.077 0.090 0.078 0.074 0.076 0.065 0.071 0.067 SPILLED 0.073 0.064 0.054 NOT TAKEN 0.067 0.079 0.092 0.078 NOT TAKEN NOT TAKEN 0.072 0.067 0.086 NOT TAKEN 0.061 NOT TAKEN 0.068 0.073 0.075 0.064 0.071 0.067 0.082 0.082 0.077 1474 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 44 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 NOT TAKEN 0.075 0.076 0.071 0.090 0.074 NOT TAKEN 0.076 0.081 0.081 0.069 0.070 0.067 0.085 NOT TAKEN 0.083 NOT TAKEN 0.079 0.070 0.075 0.074 0.073 NOT TAKEN 0.069 0.077 0.060 0.095 0.071 0.071 NOT TAKEN 0.072 0.076 0.070 0.085 0.075 NOT TAKEN 0.076 0.079 0.079 0.073 0.070 0.070 0.085 NOT TAKEN 0.082 NOT TAKEN 0.078 0.073 0.078 0.074 0.073 NOT TAKEN 0.070 0.078 0.080 SPILLED 0.079 0.071 NOT TAKEN 0.071 0.075 0.071 0.076 0.070 NOT TAKEN 0.071 0.077 0.077 0.066 0.072 0.067 0.080 NOT TAKEN 0.077 NOT TAKEN 0.078 0.069 0.075 0.071 0.070 NOT TAKEN 0.066 0.073 0.067 SPILLED 0.074 0.068 NOT TAKEN 0.070 0.074 0.065 0.072 0.068 NOT TAKEN 0.074 0.075 0.076 0.065 0.064 0.063 0.079 NOT TAKEN 0.079 NOT TAKEN 0.081 0.070 0.070 0.070 0.068 NOT TAKEN 0.067 0.074 0.058 SPILLED 0.070 0.072 NOT TAKEN 0.069 0.070 0.064 0.071 0.064 NOT TAKEN 0.068 0.074 0.074 0.065 0.064 0.068 0.077 NOT TAKEN 0.076 NOT TAKEN 0.070 0.067 0.071 0.068 0.067 NOT TAKEN 0.063 0.070 0.066 SPILLED 0.064 0.070 NOT TAKEN 0.070 0.074 0.067 0.071 0.070 NOT TAKEN 0.072 0.081 0.083 0.067 0.072 0.067 0.081 NOT TAKEN 0.080 NOT TAKEN NOT TAKEN 0.074 0.075 0.070 0.062 NOT TAKEN 0.070 0.077 0.064 SPILLED NOT TAKEN 0.072 NOT TAKEN TECH SPILL 0.074 0.069 0.073 0.068 NOT TAKEN 0.071 0.088 0.076 0.068 0.073 0.067 0.076 NOT TAKEN 0.083 NOT TAKEN NOT TAKEN 0.070 0.072 0.071 0.067 NOT TAKEN 0.070 0.078 0.066 0.087 NOT TAKEN 0.069 NOT TAKEN SPILLED 0.073 SPILLED 0.068 0.067 NOT TAKEN 0.071 0.106 0.069 0.068 0.071 0.065 0.077 NOT TAKEN 0.078 NOT TAKEN NOT TAKEN 0.068 0.071 0.067 0.074 NOT TAKEN 0.068 0.077 0.049 0.080 NOT TAKEN 0.069 NOT TAKEN 0.072 0.077 0.069 0.046 0.069 NOT TAKEN 0.068 0.111 0.077 0.066 0.070 0.067 0.075 NOT TAKEN 0.082 NOT TAKEN NOT TAKEN 0.042 0.069 0.068 0.070 NOT TAKEN 0.065 0.072 0.024 0.077 NOT TAKEN 0.066 NOT TAKEN 0.066 0.066 0.068 0.049 0.065 NOT TAKEN 0.072 0.100 0.072 0.070 0.066 0.065 0.074 NOT TAKEN 0.073 NOT TAKEN NOT TAKEN 0.052 0.066 0.066 0.065 NOT TAKEN 0.045 0.072 NOT TAKEN SPILLED NOT TAKEN 0.065 1475 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 45 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 0.189 0.172 0.185 0.181 0.199 0.219 0.215 0.173 0.209 0.268 0.181 0.197 0.199 0.173 0.195 0.166 0.186 0.194 0.182 0.195 0.181 0.190 NOT TAKEN NOT TAKEN NOT TAKEN 0.180 0.193 NOT TAKEN NOT TAKEN 0.199 0.195 0.183 0.178 0.171 0.177 0.197 0.206 0.199 0.177 0.198 0.246 0.183 0.198 0.203 0.170 0.202 0.176 0.190 0.193 0.206 0.198 0.180 0.202 NOT TAKEN NOT TAKEN NOT TAKEN 0.275 0.194 NOT TAKEN NOT TAKEN 0.203 0.210 0.181 0.142 0.182 0.167 0.182 0.195 0.203 0.173 0.205 0.239 0.181 0.190 0.174 0.163 0.194 0.175 0.190 0.195 0.198 0.197 0.179 0.200 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.192 NOT TAKEN NOT TAKEN 0.191 0.229 0.181 0.156 0.177 0.163 0.181 0.198 0.196 0.171 0.195 0.219 0.159 0.184 0.181 0.167 0.185 0.169 0.184 0.185 0.196 0.189 0.176 0.184 NOT TAKEN NOT TAKEN NOT TAKEN 0.232 0.185 NOT TAKEN NOT TAKEN 0.184 0.229 0.170 NOT TAKEN 0.171 0.160 0.178 0.194 0.185 0.167 0.201 0.217 0.043 0.185 0.180 0.162 0.180 0.161 0.181 0.180 0.173 0.183 0.164 0.181 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.185 NOT TAKEN NOT TAKEN 0.171 0.200 0.170 NOT TAKEN 0.173 0.165 0.170 0.197 0.192 0.165 0.207 0.226 NOT TAKEN 0.189 0.186 0.167 0.172 0.166 0.183 0.169 0.179 0.197 0.172 0.201 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.187 NOT TAKEN NOT TAKEN 0.180 0.206 0.189 NOT TAKEN 0.176 0.171 0.196 0.196 0.187 0.160 0.227 SPILLED NOT TAKEN 0.206 0.066 0.168 0.180 0.183 0.203 0.172 0.197 0.191 0.170 0.200 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.190 NOT TAKEN NOT TAKEN 0.167 0.222 0.170 NOT TAKEN 0.172 0.161 0.187 0.196 0.121 0.168 0.199 SPILLED NOT TAKEN 0.197 NOT TAKEN 0.155 0.169 0.151 0.180 0.174 0.182 0.183 0.172 0.174 NOT TAKEN NOT TAKEN NOT TAKEN 0.151 0.188 NOT TAKEN NOT TAKEN 0.176 0.213 0.172 NOT TAKEN 0.167 0.166 0.182 0.177 NOT TAKEN 0.164 0.198 0.220 NOT TAKEN 0.194 NOT TAKEN 0.161 0.172 0.167 0.186 0.189 0.181 0.183 0.170 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.524 0.191 NOT TAKEN NOT TAKEN 0.172 0.208 0.165 NOT TAKEN 0.161 0.161 0.182 0.190 NOT TAKEN 0.156 0.209 0.209 NOT TAKEN 0.195 NOT TAKEN 0.169 0.167 0.173 0.180 0.174 0.180 NOT TAKEN 0.157 0.189 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.198 NOT TAKEN NOT TAKEN 0.174 0.202 1476 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 46 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 NOT TAKEN 0.220 0.203 0.193 0.212 0.188 NOT TAKEN 0.190 0.189 NOT TAKEN 0.198 0.238 NOT TAKEN 0.191 0.183 0.180 0.220 0.214 0.175 0.269 0.167 0.185 NOT TAKEN 0.192 0.173 0.185 0.211 0.190 0.194 NOT TAKEN SPILLED 0.196 0.187 0.209 0.195 NOT TAKEN 0.183 0.181 NOT TAKEN 0.190 0.236 NOT TAKEN 0.192 0.184 0.189 0.201 0.225 0.185 SPILLED 0.168 0.178 NOT TAKEN 0.174 0.185 0.202 0.224 0.202 0.184 NOT TAKEN SPILLED 0.207 0.176 0.204 0.182 NOT TAKEN 0.180 0.184 NOT TAKEN 0.183 0.212 NOT TAKEN 0.191 0.180 0.179 0.191 0.208 0.183 SPILLED 0.169 0.177 NOT TAKEN 0.173 0.170 0.188 0.223 0.194 0.177 NOT TAKEN SPILLED 0.189 0.166 0.198 0.189 NOT TAKEN 0.172 0.183 NOT TAKEN 0.175 0.217 NOT TAKEN 0.183 0.177 0.180 0.189 0.208 0.178 SPILLED 0.163 0.167 NOT TAKEN 0.160 0.175 0.183 SPILLED 0.178 0.182 NOT TAKEN SPILLED 0.176 NOT TAKEN 0.197 0.170 NOT TAKEN 0.168 0.170 NOT TAKEN 0.170 0.213 NOT TAKEN 0.177 0.162 0.172 0.180 0.198 0.180 SPILLED 0.156 0.161 NOT TAKEN 0.160 0.168 0.178 SPILLED 0.173 0.167 NOT TAKEN SPILLED 0.169 NOT TAKEN 0.193 0.184 NOT TAKEN 0.181 0.176 NOT TAKEN 0.172 0.218 NOT TAKEN 0.187 0.171 0.170 0.193 0.211 0.179 SPILLED 0.159 0.175 NOT TAKEN 0.163 0.179 0.190 0.213 0.176 0.182 NOT TAKEN 0.227 0.184 NOT TAKEN 0.181 0.184 NOT TAKEN 0.161 0.183 NOT TAKEN 0.188 0.217 NOT TAKEN 0.192 0.183 0.184 0.181 0.203 0.175 SPILLED 0.164 0.161 NOT TAKEN 0.164 0.172 0.185 0.210 0.173 0.173 NOT TAKEN 0.179 0.170 NOT TAKEN 0.181 0.169 NOT TAKEN 0.148 0.182 NOT TAKEN 0.170 0.205 NOT TAKEN 0.179 0.168 0.172 0.182 0.192 0.168 SPILLED 0.160 0.140 NOT TAKEN 0.152 0.168 0.176 SPILLED 0.169 0.170 NOT TAKEN 0.208 0.176 NOT TAKEN 0.186 0.167 NOT TAKEN 0.126 0.171 NOT TAKEN 0.168 0.213 NOT TAKEN 0.171 0.165 0.171 0.187 0.197 0.176 SPILLED 0.163 0.156 NOT TAKEN 0.156 0.181 0.171 SPILLED 0.179 0.169 NOT TAKEN 0.210 0.166 NOT TAKEN 0.193 0.167 NOT TAKEN 0.124 0.176 NOT TAKEN 0.170 0.209 NOT TAKEN 0.178 0.153 0.167 0.188 0.195 0.181 0.170 0.152 0.163 NOT TAKEN 0.149 0.173 0.170 0.141 0.169 0.165 1477 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 47 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 0.642 NOT TAKEN 0.878 0.827 0.733 0.829 0.880 0.741 NOT TAKEN 0.841 0.967 0.878 0.725 0.901 NOT TAKEN 0.848 0.804 0.819 0.820 0.774 0.740 0.900 0.959 0.746 0.809 0.766 NOT TAKEN 0.769 NOT TAKEN 0.993 0.780 0.708 NOT TAKEN 0.893 0.834 0.752 0.859 SPILLED 0.755 NOT TAKEN 0.845 0.938 0.831 0.690 0.943 NOT TAKEN 0.800 0.744 0.810 0.833 0.785 0.757 0.832 0.928 0.751 0.808 0.782 NOT TAKEN 0.703 NOT TAKEN 1.001 0.779 0.733 NOT TAKEN 0.892 0.815 0.732 0.806 SPILLED 0.705 NOT TAKEN 0.838 0.921 0.809 0.666 0.904 NOT TAKEN 0.798 SPILLED 0.759 0.807 0.740 0.690 0.845 0.886 0.680 0.581 0.767 NOT TAKEN 0.740 NOT TAKEN 0.970 0.793 0.666 NOT TAKEN 0.622 0.825 0.729 0.810 0.949 0.638 NOT TAKEN 0.812 0.903 0.803 0.651 SPILLED NOT TAKEN 0.726 SPILLED 0.758 0.800 0.728 0.640 0.784 0.892 0.680 0.781 0.737 NOT TAKEN 0.700 NOT TAKEN 0.947 0.748 0.626 NOT TAKEN 0.852 0.795 0.691 0.759 SPILLED 0.549 NOT TAKEN 0.771 0.848 0.772 0.614 0.850 NOT TAKEN 0.710 0.604 0.759 0.745 0.633 0.675 NOT TAKEN 0.852 0.699 0.750 0.715 NOT TAKEN 0.533 NOT TAKEN 0.941 0.717 NOT TAKEN NOT TAKEN 0.822 NOT TAKEN NOT TAKEN 0.749 0.954 0.524 NOT TAKEN 0.766 0.859 0.741 0.640 0.843 NOT TAKEN 0.813 0.636 0.769 0.844 0.733 0.710 NOT TAKEN 0.840 0.706 0.683 0.775 NOT TAKEN NOT TAKEN NOT TAKEN 0.871 0.743 NOT TAKEN NOT TAKEN 0.816 NOT TAKEN NOT TAKEN 0.745 SPILLED 0.586 NOT TAKEN 0.795 0.850 0.731 0.626 0.864 NOT TAKEN 0.887 SPILLED 0.755 0.775 0.717 0.752 NOT TAKEN 0.861 0.698 0.761 0.770 NOT TAKEN NOT TAKEN NOT TAKEN 0.962 0.732 NOT TAKEN NOT TAKEN 0.846 NOT TAKEN NOT TAKEN 0.681 SPILLED NOT TAKEN NOT TAKEN 0.733 0.861 0.745 0.563 0.782 NOT TAKEN 0.788 0.695 0.702 0.714 0.649 0.660 NOT TAKEN 0.861 0.649 0.750 0.630 NOT TAKEN NOT TAKEN NOT TAKEN 0.938 0.774 NOT TAKEN NOT TAKEN 0.826 NOT TAKEN NOT TAKEN 0.661 0.948 NOT TAKEN NOT TAKEN 0.524 0.954 0.688 0.633 0.666 NOT TAKEN 0.846 SPILLED 0.707 0.749 0.651 0.659 NOT TAKEN 0.915 0.692 0.744 0.754 NOT TAKEN NOT TAKEN NOT TAKEN 1.016 0.806 NOT TAKEN NOT TAKEN 0.825 NOT TAKEN NOT TAKEN 0.719 0.854 NOT TAKEN NOT TAKEN 0.345 0.882 0.716 0.600 0.392 NOT TAKEN 0.789 0.630 0.695 0.740 0.633 0.634 NOT TAKEN 0.854 0.649 0.694 0.708 NOT TAKEN NOT TAKEN NOT TAKEN 0.951 0.116 1478 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 48 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 0.784 0.738 0.839 0.823 0.815 0.985 0.833 NOT TAKEN 0.769 0.898 0.749 NOT TAKEN 0.797 0.789 0.850 0.895 NOT TAKEN NOT TAKEN 0.797 0.760 0.830 0.821 0.823 0.669 0.662 0.739 0.839 0.806 0.795 NOT TAKEN NOT TAKEN 0.756 0.724 0.811 0.801 0.788 1.064 0.819 NOT TAKEN 0.788 0.850 0.757 NOT TAKEN 0.755 0.804 0.860 0.855 NOT TAKEN NOT TAKEN 0.745 0.719 0.797 0.790 0.813 0.705 0.747 0.695 0.783 0.805 0.787 NOT TAKEN NOT TAKEN 0.726 0.721 0.781 0.803 0.761 SPILLED 0.781 NOT TAKEN 0.741 0.846 0.802 NOT TAKEN 0.762 0.764 0.850 0.864 NOT TAKEN NOT TAKEN 0.819 0.676 0.850 0.836 0.803 0.739 0.800 0.747 0.800 0.781 0.777 NOT TAKEN NOT TAKEN 0.719 0.677 0.784 0.770 0.761 NOT TAKEN 0.768 NOT TAKEN 0.744 0.813 0.756 NOT TAKEN 0.733 0.744 0.840 0.762 NOT TAKEN NOT TAKEN 0.744 0.681 0.773 0.771 0.782 0.694 0.696 0.718 0.780 0.776 0.762 NOT TAKEN NOT TAKEN 0.680 0.662 0.723 0.744 0.710 NOT TAKEN 0.693 NOT TAKEN 0.738 0.736 0.712 NOT TAKEN 0.698 0.617 0.677 0.783 NOT TAKEN NOT TAKEN 0.734 0.580 0.724 0.763 0.717 0.647 0.695 0.637 0.771 0.724 0.708 NOT TAKEN NOT TAKEN 0.702 0.713 0.782 0.789 0.786 NOT TAKEN 0.723 NOT TAKEN NOT TAKEN 0.754 0.776 NOT TAKEN 0.290 NOT TAKEN NOT TAKEN 0.798 NOT TAKEN NOT TAKEN 0.717 NOT TAKEN 0.786 0.768 0.711 0.694 0.739 0.717 0.794 0.737 0.736 NOT TAKEN NOT TAKEN 0.745 0.706 0.733 0.801 0.767 NOT TAKEN 0.797 NOT TAKEN NOT TAKEN 0.791 0.750 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.841 NOT TAKEN NOT TAKEN 0.790 NOT TAKEN 0.774 0.796 0.784 0.765 0.838 0.731 0.818 0.777 0.799 NOT TAKEN NOT TAKEN 0.687 0.646 0.786 0.732 0.744 NOT TAKEN 0.729 NOT TAKEN NOT TAKEN 0.727 0.749 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.792 NOT TAKEN NOT TAKEN 0.775 NOT TAKEN 0.721 0.754 0.739 0.646 0.816 0.661 0.799 0.738 0.734 NOT TAKEN NOT TAKEN 0.679 0.677 0.763 0.774 0.782 NOT TAKEN 0.678 NOT TAKEN NOT TAKEN 0.744 0.765 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.825 NOT TAKEN NOT TAKEN 0.799 NOT TAKEN 0.740 0.757 0.744 0.408 0.784 0.679 0.788 0.722 0.782 NOT TAKEN NOT TAKEN 0.643 0.596 0.806 0.758 0.720 NOT TAKEN 0.698 NOT TAKEN NOT TAKEN 0.704 0.777 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.777 NOT TAKEN NOT TAKEN 0.803 NOT TAKEN 0.701 0.727 0.689 NOT TAKEN 0.743 0.669 0.741 0.729 0.758 NOT TAKEN NOT TAKEN 1479 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 49 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 0.847 0.834 SPILLED 0.707 0.742 0.824 0.844 1.133 0.885 0.772 1.137 0.748 0.733 0.804 0.823 1.034 0.850 0.815 SPILLED 0.739 0.748 0.801 0.863 0.720 0.817 0.788 SPILLED 0.716 0.706 0.815 0.830 1.054 0.763 0.773 SPILLED 0.681 0.500 0.737 0.779 SPILLED 0.793 0.808 SPILLED 0.747 NOT TAKEN 0.815 0.820 NOT TAKEN 0.793 0.794 1.058 0.746 NOT TAKEN 0.794 0.801 NOT TAKEN 0.805 0.746 TECH SPILL NOT TAKEN NOT TAKEN 0.753 0.768 NOT TAKEN 0.813 0.730 1.027 NOT TAKEN NOT TAKEN 0.797 0.777 NOT TAKEN 0.769 0.753 0.965 NOT TAKEN NOT TAKEN 0.759 0.748 NOT TAKEN GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 0.831 0.737 0.834 0.867 0.817 0.862 0.873 0.801 0.814 0.819 0.737 0.793 0.744 0.826 0.778 0.761 0.784 0.835 0.813 0.775 0.803 0.731 0.816 0.750 0.793 0.835 0.819 0.835 0.734 0.913 0.735 0.801 0.752 0.830 0.780 0.822 0.792 0.808 0.771 0.790 0.734 0.729 0.832 0.810 0.786 0.840 0.844 0.835 0.861 0.915 0.759 0.789 0.730 0.835 0.780 0.780 0.824 0.820 0.735 0.765 0.759 0.732 0.837 0.787 SPILLED 0.849 0.807 0.845 0.777 0.905 0.744 0.786 0.739 0.815 0.756 0.730 0.802 0.779 SPILLED 0.759 0.733 0.710 0.773 0.777 SPILLED 0.779 0.704 0.787 SPILLED 0.679 0.732 0.741 0.644 0.795 0.680 0.710 0.746 0.711 0.643 0.708 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 SPILLED 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 SPILLED NOT TAKEN 0.000 0.000 SPILLED SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN NOT TAKEN SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 1480 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 50 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 0.805 0.775 0.783 0.818 0.872 0.820 0.793 0.817 0.919 SPILLED 0.801 0.903 0.788 0.753 0.744 0.835 0.743 0.712 0.792 0.823 0.743 0.752 0.763 0.746 0.902 0.707 0.754 0.820 0.858 SPILLED 0.808 0.889 0.835 0.761 0.822 0.862 0.715 0.706 0.816 0.790 0.791 0.763 0.756 0.848 0.889 0.809 0.733 0.869 0.858 SPILLED 0.793 0.880 0.756 0.785 0.826 0.844 0.658 0.711 0.825 0.831 0.725 0.736 0.713 0.837 0.850 0.778 0.729 0.820 0.780 SPILLED 0.748 0.852 0.754 0.719 0.708 0.836 0.718 0.733 0.805 0.806 0.693 0.656 0.667 0.816 0.803 0.711 0.698 0.739 0.836 SPILLED 0.737 0.757 0.689 0.691 0.823 0.778 0.685 0.678 0.759 0.807 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 SPILLED 0.000 SPILLED 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 0.021 0.017 0.023 0.026 0.020 0.024 0.030 0.026 0.022 SPILLED 0.022 0.027 0.022 0.022 0.028 0.022 0.018 NOT TAKEN SPILLED 0.024 0.020 0.022 0.026 0.023 0.018 NOT TAKEN 0.020 0.023 0.019 0.021 0.025 0.022 0.019 NOT TAKEN 0.021 0.024 0.019 0.022 0.026 0.022 0.019 NOT TAKEN 0.021 0.025 0.019 0.024 0.013 0.021 0.020 NOT TAKEN 0.023 0.022 0.020 0.021 0.022 0.020 0.018 NOT TAKEN 0.019 0.015 0.020 0.023 0.017 0.021 0.015 NOT TAKEN 0.022 0.023 0.020 0.024 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.018 0.022 0.019 0.023 NOT TAKEN 0.017 1481 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 51 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 0.030 0.027 NOT TAKEN 0.030 0.025 NOT TAKEN NOT TAKEN 0.026 0.025 0.025 NOT TAKEN NOT TAKEN 0.030 0.027 0.024 0.030 0.023 0.023 0.026 0.028 0.035 0.033 0.022 NOT TAKEN 0.029 0.025 0.029 0.027 NOT TAKEN 0.025 NOT TAKEN 0.029 0.029 NOT TAKEN 0.028 0.024 NOT TAKEN NOT TAKEN 0.024 0.023 0.024 NOT TAKEN NOT TAKEN 0.030 SPILLED 0.028 0.030 0.021 0.021 SPILLED 0.026 0.029 0.024 0.020 NOT TAKEN 0.026 SPILLED 0.031 0.026 NOT TAKEN 0.022 NOT TAKEN 0.026 0.027 NOT TAKEN SPILLED 0.021 NOT TAKEN NOT TAKEN 0.022 0.025 0.022 NOT TAKEN NOT TAKEN 0.026 0.022 0.023 0.029 0.019 0.023 0.027 0.025 SPILLED 0.027 0.021 NOT TAKEN 0.029 0.025 0.028 0.026 NOT TAKEN 0.012 NOT TAKEN 0.024 0.026 NOT TAKEN 0.029 0.021 NOT TAKEN NOT TAKEN 0.019 0.020 0.020 NOT TAKEN NOT TAKEN 0.024 0.022 0.024 0.028 0.020 0.020 0.023 0.024 0.032 0.024 0.018 NOT TAKEN 0.024 0.023 0.028 0.023 NOT TAKEN 0.019 NOT TAKEN 0.025 0.026 NOT TAKEN NOT TAKEN 0.021 NOT TAKEN NOT TAKEN 0.018 0.022 0.022 NOT TAKEN NOT TAKEN 0.025 0.021 0.021 0.024 0.021 0.022 0.022 0.023 SPILLED 0.024 0.020 NOT TAKEN 0.026 0.022 0.029 0.024 NOT TAKEN 0.021 NOT TAKEN 0.025 0.026 NOT TAKEN NOT TAKEN 0.023 NOT TAKEN NOT TAKEN 0.017 0.022 0.023 NOT TAKEN NOT TAKEN 0.045 0.022 0.021 0.027 0.022 0.022 0.026 0.025 0.025 0.022 0.021 NOT TAKEN 0.023 0.023 0.027 0.025 NOT TAKEN 0.017 NOT TAKEN 0.024 0.021 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.018 0.024 0.018 NOT TAKEN NOT TAKEN 0.024 0.021 0.022 0.028 0.020 0.019 0.022 0.021 0.010 0.021 0.019 NOT TAKEN 0.026 0.022 0.025 0.025 NOT TAKEN 0.025 NOT TAKEN 0.028 0.024 NOT TAKEN NOT TAKEN 0.022 NOT TAKEN NOT TAKEN 0.022 0.022 0.019 NOT TAKEN NOT TAKEN 0.030 TECH SPILL 0.022 0.029 0.020 0.020 0.023 0.020 0.024 0.023 0.019 NOT TAKEN 0.022 SPILLED 0.021 0.027 NOT TAKEN 0.022 NOT TAKEN 0.026 0.026 NOT TAKEN NOT TAKEN 0.023 NOT TAKEN NOT TAKEN 0.021 0.023 0.020 NOT TAKEN NOT TAKEN 0.023 NOT TAKEN 0.022 0.025 0.020 0.020 0.022 0.020 0.022 0.021 0.019 NOT TAKEN 0.024 0.023 0.021 0.016 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.021 NOT TAKEN NOT TAKEN 0.020 0.021 0.017 NOT TAKEN NOT TAKEN 0.025 NOT TAKEN 0.021 0.025 0.018 0.019 0.020 0.020 0.022 0.018 0.018 NOT TAKEN 0.019 0.022 0.021 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1482 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 52 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0.021 0.027 0.026 0.028 0.026 0.027 0.026 0.028 NOT TAKEN 0.024 0.025 0.023 NOT TAKEN 0.024 0.023 0.022 0.026 NOT TAKEN 0.026 0.029 0.031 0.022 0.028 0.026 0.027 0.026 0.028 0.026 0.028 NOT TAKEN 0.023 0.023 0.024 NOT TAKEN 0.025 0.024 0.026 0.026 NOT TAKEN 0.027 SPILLED 0.030 0.020 0.025 0.023 0.026 0.023 0.027 0.024 0.023 NOT TAKEN 0.019 0.024 0.022 NOT TAKEN 0.022 0.024 0.022 0.025 NOT TAKEN 0.024 0.029 0.028 0.020 0.024 0.025 0.023 0.024 0.024 0.019 0.025 NOT TAKEN 0.020 0.021 0.021 NOT TAKEN 0.022 0.022 0.020 0.023 NOT TAKEN 0.024 0.026 0.026 0.020 0.025 0.025 0.022 0.023 0.025 0.024 0.024 NOT TAKEN 0.017 0.022 0.021 NOT TAKEN 0.018 0.022 0.017 0.022 NOT TAKEN 0.022 SPILLED 0.017 0.020 0.027 0.025 0.020 0.024 0.025 0.022 0.024 NOT TAKEN 0.022 0.019 0.022 NOT TAKEN 0.023 0.021 0.022 0.024 NOT TAKEN 0.020 0.028 0.024 0.019 0.024 0.020 0.023 0.022 0.024 0.016 0.022 NOT TAKEN 0.021 0.022 0.022 NOT TAKEN 0.021 0.018 0.019 0.022 NOT TAKEN 0.021 0.026 0.021 0.020 0.025 0.023 0.020 0.023 0.026 0.019 0.020 NOT TAKEN 0.020 NOT TAKEN 0.022 NOT TAKEN 0.022 0.023 0.021 0.026 NOT TAKEN 0.021 0.026 0.023 0.019 0.026 0.022 0.023 0.021 0.028 0.024 0.023 NOT TAKEN 0.020 NOT TAKEN 0.015 NOT TAKEN 0.022 0.019 0.025 0.022 NOT TAKEN 0.021 0.025 0.024 0.017 0.021 0.023 0.021 0.022 0.025 0.023 0.023 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.022 0.023 0.021 0.022 NOT TAKEN 0.019 0.021 0.022 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 0.120 0.129 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.112 0.113 0.113 NOT TAKEN 0.121 NOT TAKEN NOT TAKEN 0.112 0.093 0.101 NOT TAKEN 0.081 NOT TAKEN NOT TAKEN 0.102 0.096 0.098 NOT TAKEN 0.099 NOT TAKEN NOT TAKEN 0.109 0.098 0.107 NOT TAKEN 0.100 NOT TAKEN NOT TAKEN 0.107 0.097 0.091 NOT TAKEN 0.095 NOT TAKEN NOT TAKEN 0.104 0.085 0.092 NOT TAKEN 0.071 NOT TAKEN NOT TAKEN 0.098 0.095 0.090 NOT TAKEN 0.104 NOT TAKEN NOT TAKEN 0.101 0.089 0.094 NOT TAKEN 0.097 NOT TAKEN NOT TAKEN 0.099 0.087 0.087 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.092 1483 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 0.095 0.099 NOT TAKEN 0.112 0.110 0.098 0.087 0.122 NOT TAKEN 0.101 NOT TAKEN SPILLED 0.118 0.090 0.117 0.097 0.102 0.108 0.132 0.104 NOT TAKEN 0.087 0.114 0.094 0.085 NOT TAKEN 0.106 0.105 0.121 0.104 0.113 0.035 0.100 NOT TAKEN 0.121 0.108 0.108 0.111 0.096 NOT TAKEN 0.100 NOT TAKEN 0.106 0.114 0.088 0.107 0.106 0.098 0.110 0.123 0.100 NOT TAKEN 0.084 0.117 0.102 0.099 NOT TAKEN 0.108 0.108 0.118 0.106 0.117 TECH SPILL 0.095 NOT TAKEN 0.097 0.104 0.087 0.094 0.091 NOT TAKEN 0.089 NOT TAKEN SPILLED 0.078 0.089 0.103 0.092 0.091 0.106 0.114 0.103 NOT TAKEN SPILLED 0.077 0.088 0.083 NOT TAKEN 0.106 0.087 0.105 0.098 0.107 NOT TAKEN 0.087 NOT TAKEN 0.109 0.107 0.078 0.092 0.097 NOT TAKEN 0.090 NOT TAKEN 0.092 0.084 0.084 0.108 0.089 0.081 0.098 0.124 0.106 NOT TAKEN NOT TAKEN 0.097 0.090 0.072 NOT TAKEN 0.104 0.099 0.101 0.105 0.107 NOT TAKEN 0.088 NOT TAKEN 0.096 0.108 0.095 0.081 0.101 NOT TAKEN 0.092 NOT TAKEN 0.091 0.089 0.081 0.101 0.091 0.076 0.114 0.107 0.099 NOT TAKEN NOT TAKEN 0.081 0.082 0.066 NOT TAKEN 0.100 0.091 0.104 0.090 0.109 NOT TAKEN 0.088 NOT TAKEN 0.094 0.101 0.089 0.086 0.104 NOT TAKEN 0.090 NOT TAKEN 0.092 0.093 0.082 0.104 0.085 0.076 0.098 0.122 0.104 NOT TAKEN NOT TAKEN 0.091 0.042 0.083 NOT TAKEN 0.092 0.087 0.099 0.101 0.093 NOT TAKEN 0.092 NOT TAKEN 0.098 0.109 0.088 0.083 0.094 NOT TAKEN 0.085 NOT TAKEN 0.083 0.083 0.080 0.098 0.088 0.081 0.104 0.117 0.103 NOT TAKEN NOT TAKEN 0.064 0.041 0.079 NOT TAKEN 0.093 0.089 0.101 0.087 0.090 NOT TAKEN 0.090 NOT TAKEN 0.091 0.099 0.088 0.079 0.084 NOT TAKEN 0.088 NOT TAKEN 0.083 0.083 0.077 0.101 0.084 0.074 0.090 0.072 SPILLED NOT TAKEN NOT TAKEN 0.026 NOT TAKEN 0.078 NOT TAKEN 0.089 0.068 0.100 0.085 0.103 NOT TAKEN 0.100 NOT TAKEN 0.094 0.102 0.094 0.058 0.097 NOT TAKEN 0.080 NOT TAKEN 0.081 0.080 0.087 0.089 0.084 0.048 0.072 0.125 0.109 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.078 NOT TAKEN 0.092 0.086 0.074 0.090 0.092 NOT TAKEN 0.083 NOT TAKEN 0.087 0.091 0.078 0.070 0.090 NOT TAKEN 0.073 NOT TAKEN 0.079 0.083 0.079 SPILLED 0.078 0.044 0.108 SPILLED 0.101 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.051 NOT TAKEN 0.082 SPILLED 0.103 0.085 0.083 1484 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 54 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 SPILLED 0.098 NOT TAKEN 0.101 0.120 0.104 NOT TAKEN 0.092 0.098 0.116 0.098 0.128 0.104 0.098 0.109 0.121 NOT TAKEN 0.110 0.087 CNTMD 0.103 0.127 SPILLED 0.091 NOT TAKEN 0.105 0.106 0.110 NOT TAKEN 0.102 0.108 0.121 0.103 0.125 0.109 0.095 0.103 0.108 NOT TAKEN 0.117 0.097 0.125 0.096 0.115 SPILLED 0.088 NOT TAKEN 0.093 0.126 0.098 NOT TAKEN 0.101 0.084 0.105 0.088 0.145 0.102 0.091 0.112 0.103 NOT TAKEN 0.109 0.091 0.105 0.089 0.108 0.129 0.082 NOT TAKEN 0.103 0.125 0.103 NOT TAKEN 0.097 0.098 0.104 0.083 0.123 0.105 0.087 0.115 0.100 NOT TAKEN 0.110 0.078 0.106 0.101 0.107 SPILLED 0.082 NOT TAKEN 0.094 0.067 0.103 NOT TAKEN 0.089 0.094 0.109 0.098 0.113 0.100 0.078 0.123 0.102 NOT TAKEN 0.087 0.075 0.098 0.091 0.107 0.126 0.081 NOT TAKEN 0.092 0.131 0.108 NOT TAKEN 0.079 0.081 0.104 0.083 0.107 0.104 0.093 SPILLED 0.094 NOT TAKEN 0.104 0.084 0.118 0.089 0.102 0.130 0.077 NOT TAKEN 0.089 SPILLED 0.103 NOT TAKEN 0.087 0.080 0.088 0.083 0.127 0.097 0.088 0.108 0.042 NOT TAKEN 0.100 0.074 0.108 0.093 0.098 SPILLED 0.077 NOT TAKEN 0.085 0.070 0.105 NOT TAKEN 0.080 0.053 0.078 0.079 0.126 0.096 0.087 0.105 0.048 NOT TAKEN 0.093 0.080 0.104 0.048 0.094 SPILLED 0.084 NOT TAKEN 0.087 NOT TAKEN 0.109 NOT TAKEN 0.077 NOT TAKEN 0.079 0.083 SPILLED 0.095 0.078 SPILLED NOT TAKEN NOT TAKEN 0.087 0.076 0.104 0.042 0.094 NOT TAKEN 0.072 NOT TAKEN 0.075 NOT TAKEN 0.095 NOT TAKEN 0.076 NOT TAKEN NOT TAKEN 0.080 0.122 0.090 0.082 SPILLED NOT TAKEN NOT TAKEN 0.081 0.076 0.095 NOT TAKEN 0.081 GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 NOT TAKEN 0.237 0.250 0.309 0.221 0.286 NOT TAKEN 0.254 0.232 0.314 0.258 0.277 NOT TAKEN 0.229 0.213 0.275 0.232 0.266 NOT TAKEN 0.225 0.221 0.281 0.229 0.260 NOT TAKEN 0.226 0.225 0.263 0.221 0.246 NOT TAKEN 0.203 0.229 0.248 0.197 0.251 NOT TAKEN 0.181 0.121 0.215 0.201 0.233 NOT TAKEN 0.123 0.153 0.203 0.201 0.238 NOT TAKEN 0.117 NOT TAKEN 0.259 0.192 0.248 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.187 0.229 1485 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 55 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 0.263 NOT TAKEN 0.269 0.239 0.269 0.291 0.249 NOT TAKEN 0.306 SPILLED NOT TAKEN 0.270 0.240 NOT TAKEN NOT TAKEN 0.253 0.278 0.216 NOT TAKEN 0.269 0.254 0.289 0.244 SPILLED 0.236 0.237 0.252 NOT TAKEN NOT TAKEN 0.267 0.246 0.268 NOT TAKEN 0.259 0.296 0.254 0.276 0.242 NOT TAKEN 0.291 SPILLED NOT TAKEN 0.273 0.255 NOT TAKEN NOT TAKEN 0.234 0.296 0.217 NOT TAKEN 0.256 0.240 0.266 0.250 SPILLED 0.255 0.240 0.273 NOT TAKEN NOT TAKEN 0.257 0.273 0.223 NOT TAKEN 0.235 0.257 0.260 0.259 0.223 NOT TAKEN 0.282 SPILLED NOT TAKEN 0.251 0.249 NOT TAKEN NOT TAKEN 0.226 0.269 0.126 NOT TAKEN 0.255 0.248 0.268 0.267 SPILLED 0.223 0.219 0.262 NOT TAKEN NOT TAKEN 0.255 0.264 0.250 NOT TAKEN 0.247 0.267 0.249 0.259 0.227 NOT TAKEN 0.295 0.274 NOT TAKEN 0.262 0.236 NOT TAKEN NOT TAKEN 0.242 SPILLED 0.275 NOT TAKEN 0.221 0.231 0.257 0.249 0.257 0.251 0.231 0.244 NOT TAKEN NOT TAKEN 0.231 0.232 0.226 NOT TAKEN 0.217 0.298 0.249 0.261 0.206 NOT TAKEN 0.282 SPILLED NOT TAKEN 0.243 0.245 NOT TAKEN NOT TAKEN 0.232 0.328 0.219 NOT TAKEN 0.210 0.193 0.247 0.239 SPILLED 0.230 0.196 0.235 NOT TAKEN NOT TAKEN 0.224 0.231 0.227 NOT TAKEN 0.225 0.262 0.266 0.251 0.216 NOT TAKEN 0.259 0.289 NOT TAKEN 0.260 0.220 NOT TAKEN NOT TAKEN 0.212 0.286 0.204 NOT TAKEN 0.234 0.226 0.340 0.230 SPILLED 0.265 0.210 0.226 NOT TAKEN NOT TAKEN 0.216 0.236 0.223 NOT TAKEN 0.234 0.251 0.263 0.248 0.206 NOT TAKEN 0.261 0.222 NOT TAKEN 0.228 0.216 NOT TAKEN NOT TAKEN 0.202 0.257 0.188 NOT TAKEN 0.212 0.186 0.225 0.187 0.223 0.225 0.222 0.212 NOT TAKEN NOT TAKEN 0.222 0.214 0.225 NOT TAKEN 0.223 0.235 SPILLED 0.242 0.201 NOT TAKEN 0.268 SPILLED NOT TAKEN 0.233 0.214 NOT TAKEN NOT TAKEN 0.224 0.238 0.187 NOT TAKEN 0.180 0.273 0.236 0.219 SPILLED 0.201 0.226 0.217 NOT TAKEN NOT TAKEN 0.231 0.222 0.244 NOT TAKEN 0.213 0.259 0.272 0.233 0.223 NOT TAKEN 0.257 SPILLED NOT TAKEN 0.238 0.224 NOT TAKEN NOT TAKEN 0.236 0.266 0.182 NOT TAKEN 0.250 0.255 0.240 0.222 0.256 0.220 0.217 0.232 NOT TAKEN NOT TAKEN 0.209 0.250 0.225 NOT TAKEN 0.202 0.180 0.221 0.225 0.189 NOT TAKEN 0.212 SPILLED NOT TAKEN 0.242 0.190 NOT TAKEN NOT TAKEN 0.226 0.128 0.168 NOT TAKEN 0.211 0.238 0.235 0.192 SPILLED 0.194 0.215 0.225 NOT TAKEN NOT TAKEN 0.220 0.207 1486 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 56 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.282 0.260 0.253 0.251 0.298 0.274 0.258 0.254 NOT TAKEN 0.295 0.261 0.250 0.256 0.241 0.296 0.316 0.248 0.266 0.257 0.226 0.242 NOT TAKEN 0.241 0.291 0.289 0.261 0.258 0.314 0.260 0.275 0.246 NOT TAKEN 0.276 0.228 0.239 0.313 0.227 0.284 0.304 0.215 0.269 0.247 0.255 0.243 NOT TAKEN 0.252 0.251 0.213 0.267 0.233 0.272 0.232 0.242 0.245 NOT TAKEN 0.256 0.228 0.221 0.273 0.262 0.281 0.257 0.227 0.257 0.256 0.219 0.214 NOT TAKEN 0.246 0.262 0.273 0.247 0.244 0.276 0.254 0.233 0.235 NOT TAKEN 0.249 0.204 0.222 0.268 0.255 0.281 0.257 0.227 0.260 0.241 SPILLED 0.221 NOT TAKEN 0.216 0.290 0.253 0.226 0.235 0.271 0.213 0.231 0.223 NOT TAKEN SPILLED 0.202 0.223 0.241 0.207 0.269 0.234 0.210 0.246 0.203 0.228 NOT TAKEN NOT TAKEN 0.248 0.293 0.268 0.237 0.228 0.269 0.225 0.235 0.222 NOT TAKEN 0.230 0.224 0.215 0.251 0.204 0.254 0.231 0.221 0.252 0.204 0.210 NOT TAKEN NOT TAKEN 0.210 0.285 0.236 0.242 0.225 0.237 0.210 0.208 0.216 NOT TAKEN 0.234 0.245 0.201 0.247 0.205 0.241 0.234 0.211 0.238 0.228 0.224 NOT TAKEN NOT TAKEN 0.250 0.293 0.233 0.249 0.221 SPILLED 0.206 0.228 0.209 NOT TAKEN 0.227 0.210 0.199 0.256 0.192 0.289 0.230 0.202 0.253 0.210 0.232 NOT TAKEN NOT TAKEN 0.201 0.208 0.232 0.244 0.218 0.266 0.192 0.210 0.228 NOT TAKEN 0.267 0.211 0.192 0.238 0.191 0.261 0.229 0.167 0.247 0.216 0.222 NOT TAKEN NOT TAKEN 0.188 0.166 0.246 0.222 0.240 0.125 0.223 0.200 0.207 NOT TAKEN 0.207 0.209 0.183 0.230 0.217 0.225 0.211 0.231 SPILLED 0.229 0.209 NOT TAKEN NOT TAKEN 0.223 GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 NOT TAKEN 1.040 1.173 NOT TAKEN 1.257 NOT TAKEN 1.061 1.266 NOT TAKEN 1.215 NOT TAKEN SPILLED 1.108 NOT TAKEN 1.116 NOT TAKEN 1.137 1.043 NOT TAKEN 1.080 NOT TAKEN 0.855 1.127 NOT TAKEN 1.067 NOT TAKEN NOT TAKEN 1.032 NOT TAKEN 1.034 NOT TAKEN NOT TAKEN 0.959 NOT TAKEN 0.968 NOT TAKEN NOT TAKEN 1.006 NOT TAKEN 0.997 NOT TAKEN NOT TAKEN 0.969 NOT TAKEN 0.978 NOT TAKEN NOT TAKEN 0.980 NOT TAKEN 0.863 1487 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 57 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 1.220 1.229 1.058 1.143 1.207 1.050 1.034 NOT TAKEN 1.030 1.216 1.103 NOT TAKEN 1.004 1.208 1.049 1.097 1.049 1.070 1.066 1.257 1.242 1.246 0.957 1.326 1.123 1.118 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.984 1.242 1.198 1.163 1.199 1.255 1.117 1.122 NOT TAKEN 1.074 1.266 1.169 NOT TAKEN 1.134 1.314 1.140 1.019 0.983 1.414 0.998 1.321 1.298 1.180 1.018 1.172 1.095 1.123 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.207 1.212 1.104 1.129 1.123 1.141 SPILLED 1.049 NOT TAKEN 1.215 1.257 1.065 NOT TAKEN 1.105 1.255 0.973 1.028 0.931 SPILLED 0.990 1.140 1.201 1.192 0.889 1.072 0.922 1.023 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.055 1.203 SPILLED 1.081 1.116 1.112 1.067 1.126 NOT TAKEN 1.119 1.163 1.059 NOT TAKEN 1.043 1.251 0.999 0.993 0.972 1.183 1.007 1.195 1.232 1.171 0.944 1.123 0.924 0.955 NOT TAKEN NOT TAKEN NOT TAKEN 1.354 1.400 1.116 1.398 1.006 1.173 0.991 1.111 1.040 NOT TAKEN 1.151 1.189 1.024 NOT TAKEN 1.013 1.294 1.031 0.959 0.811 0.896 1.011 1.180 1.256 1.030 1.028 1.070 0.987 1.020 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.115 NOT TAKEN NOT TAKEN 0.980 1.092 NOT TAKEN 1.081 0.967 NOT TAKEN 1.080 1.163 0.599 NOT TAKEN 0.957 1.156 NOT TAKEN 0.919 0.900 1.171 0.897 1.301 NOT TAKEN 0.914 NOT TAKEN 1.005 0.952 0.963 NOT TAKEN NOT TAKEN NOT TAKEN 1.240 1.126 NOT TAKEN NOT TAKEN 1.014 1.028 NOT TAKEN 1.090 0.989 NOT TAKEN 1.087 1.210 NOT TAKEN NOT TAKEN 0.959 1.091 NOT TAKEN 0.835 0.909 0.837 0.650 SPILLED NOT TAKEN 1.066 NOT TAKEN 1.034 0.977 0.894 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.194 NOT TAKEN NOT TAKEN 1.093 SPILLED NOT TAKEN 3.483 0.961 NOT TAKEN 1.101 1.179 NOT TAKEN NOT TAKEN 1.108 SPILLED NOT TAKEN 0.856 0.905 0.987 0.658 1.314 NOT TAKEN 0.927 NOT TAKEN 0.983 0.981 1.105 NOT TAKEN NOT TAKEN NOT TAKEN 1.133 1.133 NOT TAKEN NOT TAKEN 1.287 1.245 NOT TAKEN 1.118 1.006 NOT TAKEN 1.026 1.179 NOT TAKEN NOT TAKEN 1.043 1.396 NOT TAKEN 0.940 0.947 1.047 NOT TAKEN 1.401 NOT TAKEN 1.003 NOT TAKEN 0.931 0.965 0.960 NOT TAKEN NOT TAKEN NOT TAKEN 1.059 1.253 NOT TAKEN NOT TAKEN 1.120 1.268 NOT TAKEN 0.857 1.038 NOT TAKEN 1.022 1.084 NOT TAKEN NOT TAKEN 0.985 1.163 NOT TAKEN 0.856 0.806 0.935 NOT TAKEN SPILLED NOT TAKEN 0.974 NOT TAKEN 0.974 0.887 0.936 NOT TAKEN NOT TAKEN NOT TAKEN 1.270 1.173 1488 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 58 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 1.043 1.164 1.026 1.310 1.162 1.151 1.224 1.199 NOT TAKEN 1.127 1.154 1.130 NOT TAKEN 1.203 1.152 1.053 1.118 1.055 1.126 0.867 0.907 1.105 NOT TAKEN 0.858 1.108 1.046 1.041 SPILLED 1.091 1.051 1.167 1.113 1.189 1.212 1.252 1.437 1.194 1.287 1.166 NOT TAKEN 1.078 1.215 1.526 NOT TAKEN 1.245 0.996 1.121 1.133 1.147 1.061 1.182 NOT TAKEN 1.170 NOT TAKEN SPILLED 1.715 1.075 1.137 SPILLED 1.107 0.995 1.172 1.052 1.182 1.042 1.240 1.042 1.164 1.262 1.014 NOT TAKEN 1.024 1.101 1.137 NOT TAKEN 1.192 1.040 0.809 0.950 1.210 0.975 0.772 NOT TAKEN 1.049 NOT TAKEN SPILLED 1.054 1.005 1.013 SPILLED 1.065 0.888 1.109 0.980 1.171 1.014 1.202 1.036 1.241 1.262 1.093 NOT TAKEN 1.022 1.011 1.100 NOT TAKEN SPILLED 1.086 0.999 1.016 1.181 0.996 0.807 NOT TAKEN 1.091 NOT TAKEN 1.624 1.014 1.012 0.999 1.420 1.060 0.874 1.116 1.073 1.115 0.984 1.211 1.077 1.358 1.243 0.967 NOT TAKEN 0.971 1.029 1.140 NOT TAKEN SPILLED 1.025 1.008 0.924 1.192 1.004 1.055 NOT TAKEN 1.177 NOT TAKEN 1.200 0.927 0.972 0.947 1.376 1.072 0.899 1.120 NOT TAKEN 1.149 0.975 1.120 1.060 1.020 1.089 NOT TAKEN NOT TAKEN 1.003 0.982 1.017 NOT TAKEN 1.190 0.964 1.074 NOT TAKEN 1.041 0.988 0.812 NOT TAKEN 1.114 NOT TAKEN 1.265 NOT TAKEN 1.096 1.058 SPILLED 1.019 0.924 1.255 NOT TAKEN 1.156 0.988 1.161 1.109 1.043 1.136 NOT TAKEN NOT TAKEN 0.972 0.959 0.975 NOT TAKEN 1.112 0.868 1.119 NOT TAKEN 1.112 0.965 0.893 NOT TAKEN 1.051 NOT TAKEN SPILLED NOT TAKEN 0.892 0.964 0.958 1.028 0.831 1.113 NOT TAKEN 1.068 0.984 1.184 1.111 1.071 1.153 NOT TAKEN NOT TAKEN 1.061 0.960 0.953 NOT TAKEN SPILLED 0.986 1.030 NOT TAKEN 1.064 1.004 0.975 NOT TAKEN 1.118 NOT TAKEN SPILLED NOT TAKEN 1.075 SPILLED SPILLED 1.051 0.879 1.146 NOT TAKEN 1.022 1.060 1.201 1.147 NOT TAKEN 1.248 NOT TAKEN NOT TAKEN 0.948 0.934 0.955 NOT TAKEN SPILLED 0.765 1.095 NOT TAKEN 1.133 1.004 0.852 NOT TAKEN 1.070 NOT TAKEN SPILLED NOT TAKEN 0.948 0.964 1.078 0.989 0.899 1.275 NOT TAKEN 0.982 0.903 1.175 1.124 NOT TAKEN 1.201 NOT TAKEN NOT TAKEN 0.958 0.862 0.939 NOT TAKEN 0.081 0.981 SPILLED NOT TAKEN 1.015 0.941 0.857 NOT TAKEN 1.005 NOT TAKEN SPILLED NOT TAKEN 0.425 0.941 0.918 1.028 0.753 1.073 1489 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 59 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93178 C93179 C93180 1.033 1.156 1.082 1.024 1.207 1.106 0.907 1.050 1.011 0.995 1.073 1.130 0.918 1.015 1.043 0.909 1.135 0.911 0.886 1.011 0.666 0.928 1.035 NOT TAKEN 0.885 1.029 NOT TAKEN 0.882 1.012 NOT TAKEN GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 1.134 1.097 1.031 1.231 1.137 1.209 1.036 1.055 1.049 1.156 1.288 1.238 1.016 1.082 1.065 1.111 1.140 0.983 1.118 1.174 1.047 1.044 1.119 1.100 1.070 1.171 1.122 0.859 1.214 1.112 1.222 1.054 1.091 1.255 1.193 1.210 1.296 1.134 1.074 1.124 1.135 1.275 1.017 1.097 1.204 1.263 0.973 1.065 1.129 1.044 1.071 1.027 0.784 1.190 1.149 1.362 0.964 1.017 1.571 1.125 1.095 1.155 0.972 1.014 1.045 1.118 1.093 1.001 1.056 1.102 1.061 0.986 0.998 1.099 0.975 1.094 1.052 0.957 1.164 1.087 1.199 0.935 0.958 1.320 1.155 0.984 1.180 0.951 0.968 1.009 1.050 1.265 0.955 1.026 1.114 1.410 1.013 0.993 1.141 1.071 1.131 1.076 0.933 1.132 1.046 1.202 0.963 0.952 1.262 1.108 0.964 1.114 0.895 1.053 0.998 1.098 1.254 0.960 0.999 1.133 1.052 0.930 1.127 1.076 1.037 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 1490 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 60 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 36 40 44 48 52 56 60 64 68 72 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 1.156 1.211 1.225 SPILLED 0.987 1.169 1.096 1.061 1.146 1.000 1.095 1.141 1.073 0.938 0.983 1.206 SPILLED 1.054 SPILLED 1.043 1.192 1.185 1.076 1.158 1.039 1.051 1.107 1.151 1.004 1.042 1.186 NOT TAKEN 1.073 SPILLED 1.006 1.135 1.072 0.854 1.098 0.983 1.024 1.014 1.039 0.997 0.976 1.167 NOT TAKEN 1.057 SPILLED 0.954 1.163 0.998 0.874 1.010 0.906 0.992 0.998 1.020 0.924 0.936 1.103 NOT TAKEN 0.910 SPILLED 0.994 1.112 0.978 1.240 1.026 0.975 1.013 1.000 1.076 0.918 0.928 0.000 NOT TAKEN 0.000 SPILLED SPILLED SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 1491 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 61 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 0.017 0.016 0.015 NOT TAKEN NOT TAKEN 0.016 0.014 0.014 0.013 0.005 0.018 0.017 0.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.019 0.019 0.017 0.017 0.015 NOT TAKEN 0.018 0.016 NOT TAKEN 0.017 0.017 NOT TAKEN NOT TAKEN 0.016 0.018 0.017 0.009 NOT TAKEN NOT TAKEN 0.014 0.012 0.012 0.010 NOT TAKEN 0.015 0.014 0.015 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.018 0.016 0.016 0.016 0.015 NOT TAKEN 0.017 0.015 NOT TAKEN 0.018 0.017 NOT TAKEN NOT TAKEN 0.018 0.017 0.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.015 0.006 0.013 NOT TAKEN 0.017 0.017 0.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.011 0.021 0.017 0.017 0.016 0.016 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.017 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.015 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.017 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.021 0.018 0.017 0.016 NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.014 0.017 NOT TAKEN NOT TAKEN 0.016 0.016 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.015 NOT TAKEN NOT TAKEN NOT TAKEN 0.014 0.016 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.023 0.016 0.017 0.015 NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN 0.017 0.017 NOT TAKEN NOT TAKEN 0.017 0.009 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.011 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.011 0.014 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.013 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.017 NOT TAKEN NOT TAKEN 0.017 SPILLED 0.008 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.023 SPILLED 0.016 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.011 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.016 NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.013 NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN 0.015 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.015 NOT TAKEN NOT TAKEN 0.016 1492 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 62 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 2 - 0.5 PPM C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 0.022 NOT TAKEN 0.009 0.015 0.018 0.018 0.003 0.017 NOT TAKEN 0.018 0.004 NOT TAKEN SPILLED 0.014 NOT TAKEN 0.013 NOT TAKEN 0.018 0.020 NOT TAKEN 0.016 0.016 NOT TAKEN 0.015 0.017 NOT TAKEN NOT TAKEN 0.011 0.020 0.026 NOT TAKEN 0.012 0.016 NOT TAKEN 0.018 NOT TAKEN 0.018 NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED 0.014 NOT TAKEN 0.008 NOT TAKEN 0.018 0.020 NOT TAKEN 0.014 0.015 NOT TAKEN 0.011 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.023 NOT TAKEN 0.012 0.016 NOT TAKEN 0.010 NOT TAKEN 0.018 NOT TAKEN 0.015 NOT TAKEN NOT TAKEN SPILLED 0.013 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 0.024 NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.008 0.022 NOT TAKEN 0.013 0.016 NOT TAKEN 0.011 NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.005 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 0.022 NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.020 NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 0.015 NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1493 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 63 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 0.071 0.066 0.069 0.068 0.013 SPILLED 0.075 0.065 0.047 NOT TAKEN 0.064 0.060 0.084 0.076 NOT TAKEN NOT TAKEN 0.060 0.062 0.079 NOT TAKEN 0.045 NOT TAKEN 0.065 0.041 0.072 0.066 0.070 0.063 0.070 0.091 0.084 0.073 0.063 0.067 0.069 NOT TAKEN SPILLED 0.072 0.065 0.089 NOT TAKEN 0.063 0.061 0.082 0.077 NOT TAKEN NOT TAKEN NOT TAKEN 0.063 SPILLED NOT TAKEN 0.030 NOT TAKEN 0.065 NOT TAKEN 0.072 0.064 0.065 0.069 0.072 0.084 0.096 0.073 0.065 0.066 0.067 NOT TAKEN 0.063 0.073 0.048 0.020 NOT TAKEN 0.066 0.066 0.085 0.075 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.077 NOT TAKEN NOT TAKEN NOT TAKEN 0.066 NOT TAKEN 0.071 0.070 0.071 0.069 SPILLED 0.089 0.010 0.069 0.063 0.063 NOT TAKEN NOT TAKEN SPILLED 0.075 0.056 NOT TAKEN NOT TAKEN 0.066 0.063 0.072 0.079 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.082 NOT TAKEN NOT TAKEN NOT TAKEN 0.060 NOT TAKEN 0.059 0.069 0.071 0.064 0.057 0.079 0.076 0.073 SPILLED 0.066 NOT TAKEN NOT TAKEN SPILLED 0.077 NOT TAKEN NOT TAKEN NOT TAKEN 0.072 0.066 0.074 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.088 NOT TAKEN NOT TAKEN NOT TAKEN 0.063 NOT TAKEN 0.049 0.040 0.075 0.061 0.063 SPILLED NOT TAKEN 0.073 0.069 0.072 NOT TAKEN NOT TAKEN SPILLED 0.075 NOT TAKEN NOT TAKEN NOT TAKEN 0.080 0.065 0.082 0.072 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.082 NOT TAKEN NOT TAKEN NOT TAKEN 0.061 NOT TAKEN NOT TAKEN 0.020 0.072 NOT TAKEN SPILLED 0.087 NOT TAKEN 0.071 0.070 0.070 NOT TAKEN NOT TAKEN NOT TAKEN 0.090 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.067 0.072 0.071 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.062 NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN SPILLED 0.085 NOT TAKEN 0.070 NOT TAKEN 0.082 NOT TAKEN NOT TAKEN NOT TAKEN 0.115 NOT TAKEN NOT TAKEN NOT TAKEN 0.035 0.067 0.073 0.060 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN NOT TAKEN 0.067 NOT TAKEN SPILLED 0.082 NOT TAKEN 1494 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 64 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 NOT TAKEN 0.071 0.032 0.065 NOT TAKEN 0.002 NOT TAKEN 0.065 NOT TAKEN 0.075 0.064 0.072 0.066 0.071 NOT TAKEN 0.076 NOT TAKEN NOT TAKEN 0.074 0.071 0.067 0.063 NOT TAKEN 0.051 0.074 NOT TAKEN SPILLED NOT TAKEN 0.065 NOT TAKEN 0.067 NOT TAKEN 0.065 NOT TAKEN NOT TAKEN NOT TAKEN 0.069 NOT TAKEN 0.070 0.062 0.073 0.064 0.070 NOT TAKEN 0.068 NOT TAKEN NOT TAKEN 0.057 0.069 0.073 0.066 NOT TAKEN NOT TAKEN 0.054 NOT TAKEN SPILLED NOT TAKEN 0.065 NOT TAKEN 0.064 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.071 NOT TAKEN 0.084 0.070 0.069 0.034 0.071 NOT TAKEN 0.074 NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.068 0.064 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.057 NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.067 NOT TAKEN 0.075 0.064 0.064 0.060 0.067 NOT TAKEN 0.075 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.076 0.065 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.068 NOT TAKEN 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN 0.068 0.052 0.058 0.060 0.061 NOT TAKEN 0.075 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.048 NOT TAKEN 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.053 NOT TAKEN 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.063 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.061 NOT TAKEN 0.062 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.062 0.040 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 0.059 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.065 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.060 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1495 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 65 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 0.160 NOT TAKEN SPILLED 0.157 0.172 0.169 NOT TAKEN 0.151 0.210 0.190 NOT TAKEN 0.184 NOT TAKEN 0.159 0.167 0.162 0.174 0.177 0.175 NOT TAKEN 0.152 0.170 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.196 NOT TAKEN NOT TAKEN 0.171 0.217 0.166 NOT TAKEN 0.168 0.161 0.180 0.203 NOT TAKEN 0.152 0.198 0.209 NOT TAKEN 0.184 NOT TAKEN 0.160 0.158 SPILLED 0.176 0.157 0.162 NOT TAKEN 0.165 0.154 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.186 NOT TAKEN NOT TAKEN 0.123 NOT TAKEN 0.169 NOT TAKEN 0.173 0.164 0.177 0.201 NOT TAKEN 0.163 0.189 0.223 NOT TAKEN 0.190 NOT TAKEN 0.171 0.164 0.163 0.179 0.197 0.137 NOT TAKEN 0.174 0.150 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.193 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.176 NOT TAKEN 0.174 0.157 0.185 0.198 NOT TAKEN 0.159 0.181 0.201 NOT TAKEN 0.176 NOT TAKEN 0.165 0.163 0.192 0.032 0.200 0.162 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.199 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.159 NOT TAKEN 0.170 0.158 0.168 0.184 NOT TAKEN 0.152 0.179 0.231 NOT TAKEN 0.184 NOT TAKEN 0.157 0.164 SPILLED NOT TAKEN 0.180 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.188 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.173 NOT TAKEN 0.140 0.157 0.176 0.191 NOT TAKEN 0.141 0.176 0.210 NOT TAKEN 0.191 NOT TAKEN 0.164 0.169 0.139 NOT TAKEN 0.180 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.232 0.173 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.172 NOT TAKEN 0.140 0.151 0.176 0.187 NOT TAKEN 0.096 0.191 0.176 NOT TAKEN 0.127 NOT TAKEN 0.154 0.183 NOT TAKEN NOT TAKEN 0.179 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.176 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.153 NOT TAKEN 0.148 0.152 0.185 0.188 NOT TAKEN 0.013 0.185 0.149 NOT TAKEN 0.117 NOT TAKEN 0.148 0.175 NOT TAKEN NOT TAKEN 0.197 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.193 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1496 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 66 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 NOT TAKEN SPILLED 0.160 NOT TAKEN 0.189 0.164 NOT TAKEN 0.118 0.173 NOT TAKEN 0.163 0.191 NOT TAKEN 0.178 0.162 0.166 0.201 0.192 0.169 SPILLED SPILLED 0.163 NOT TAKEN 0.154 0.188 0.173 SPILLED 0.171 0.173 NOT TAKEN NOT TAKEN 0.149 NOT TAKEN 0.188 0.160 NOT TAKEN NOT TAKEN 0.168 NOT TAKEN 0.161 0.199 NOT TAKEN 0.174 0.156 0.164 0.215 0.193 0.187 SPILLED 0.150 0.154 NOT TAKEN 0.144 NOT TAKEN 0.159 0.217 0.176 0.163 NOT TAKEN NOT TAKEN 0.165 NOT TAKEN 0.187 0.161 NOT TAKEN NOT TAKEN 0.170 NOT TAKEN NOT TAKEN 0.108 NOT TAKEN 0.177 0.162 0.155 0.221 0.196 0.180 0.145 0.146 NOT TAKEN NOT TAKEN 0.084 NOT TAKEN 0.167 0.205 0.180 0.169 NOT TAKEN NOT TAKEN 0.172 NOT TAKEN 0.191 0.115 NOT TAKEN NOT TAKEN 0.162 NOT TAKEN NOT TAKEN 0.036 NOT TAKEN 0.173 0.153 0.067 0.285 0.184 0.177 0.037 0.151 NOT TAKEN NOT TAKEN 0.049 NOT TAKEN SPILLED 0.139 0.168 0.168 NOT TAKEN NOT TAKEN 0.168 NOT TAKEN 0.183 NOT TAKEN NOT TAKEN NOT TAKEN 0.165 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.163 0.149 NOT TAKEN NOT TAKEN 0.187 0.155 NOT TAKEN 0.159 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.188 0.167 0.159 NOT TAKEN NOT TAKEN 0.168 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.138 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.168 0.155 NOT TAKEN NOT TAKEN 0.193 0.168 NOT TAKEN 0.157 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.176 0.173 0.172 NOT TAKEN NOT TAKEN 0.154 NOT TAKEN 0.219 NOT TAKEN NOT TAKEN NOT TAKEN 0.115 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.169 0.175 NOT TAKEN NOT TAKEN 0.186 0.172 NOT TAKEN 0.156 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.182 0.171 0.159 NOT TAKEN NOT TAKEN 0.164 NOT TAKEN 0.203 NOT TAKEN NOT TAKEN NOT TAKEN 0.106 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.171 0.160 NOT TAKEN NOT TAKEN 0.158 0.170 NOT TAKEN 0.143 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN CNTMD 0.137 NOT TAKEN 0.098 1497 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 67 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 NOT TAKEN NOT TAKEN 0.823 NOT TAKEN NOT TAKEN 0.711 0.867 NOT TAKEN NOT TAKEN NOT TAKEN 0.840 0.670 SPILLED NOT TAKEN NOT TAKEN 0.864 0.449 0.712 0.720 0.661 0.627 NOT TAKEN 0.921 0.661 0.670 0.715 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.310 NOT TAKEN NOT TAKEN 0.748 NOT TAKEN NOT TAKEN 0.671 0.767 NOT TAKEN NOT TAKEN NOT TAKEN 0.863 0.624 0.547 NOT TAKEN NOT TAKEN 0.726 0.609 0.696 0.753 SPILLED 0.095 NOT TAKEN 0.942 0.609 0.685 0.681 NOT TAKEN NOT TAKEN NOT TAKEN 0.946 NOT TAKEN NOT TAKEN NOT TAKEN 0.851 NOT TAKEN NOT TAKEN 0.313 0.766 NOT TAKEN NOT TAKEN NOT TAKEN 0.816 0.660 0.575 NOT TAKEN NOT TAKEN 0.770 0.617 0.692 0.738 SPILLED NOT TAKEN NOT TAKEN 0.852 0.665 0.724 0.755 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.812 NOT TAKEN NOT TAKEN NOT TAKEN 0.760 NOT TAKEN NOT TAKEN NOT TAKEN 0.965 0.690 0.557 NOT TAKEN NOT TAKEN 0.720 0.697 0.683 0.602 0.703 NOT TAKEN NOT TAKEN 0.904 0.695 0.667 0.763 NOT TAKEN NOT TAKEN NOT TAKEN 0.952 NOT TAKEN NOT TAKEN NOT TAKEN 0.765 NOT TAKEN NOT TAKEN NOT TAKEN 0.666 NOT TAKEN NOT TAKEN NOT TAKEN 0.881 0.680 0.203 NOT TAKEN NOT TAKEN 0.750 0.541 0.508 0.771 0.653 NOT TAKEN NOT TAKEN 0.950 0.649 0.648 0.769 NOT TAKEN NOT TAKEN NOT TAKEN 0.986 NOT TAKEN NOT TAKEN NOT TAKEN 0.795 NOT TAKEN NOT TAKEN NOT TAKEN 0.584 NOT TAKEN NOT TAKEN NOT TAKEN 1.029 0.667 NOT TAKEN NOT TAKEN NOT TAKEN 0.778 0.128 NOT TAKEN 0.723 0.678 NOT TAKEN NOT TAKEN 0.939 0.645 0.613 0.728 NOT TAKEN NOT TAKEN NOT TAKEN 0.802 NOT TAKEN NOT TAKEN NOT TAKEN 0.656 NOT TAKEN NOT TAKEN NOT TAKEN 0.643 NOT TAKEN NOT TAKEN NOT TAKEN 1.251 0.654 NOT TAKEN NOT TAKEN NOT TAKEN 0.727 NOT TAKEN NOT TAKEN 0.560 0.395 NOT TAKEN NOT TAKEN 0.993 0.694 0.573 0.690 NOT TAKEN NOT TAKEN NOT TAKEN 0.578 NOT TAKEN NOT TAKEN NOT TAKEN 0.582 NOT TAKEN NOT TAKEN NOT TAKEN 0.461 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.676 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.879 0.621 0.174 0.735 NOT TAKEN NOT TAKEN NOT TAKEN 0.357 NOT TAKEN 1498 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 68 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 0.667 0.393 0.506 0.693 0.730 NOT TAKEN 0.745 NOT TAKEN NOT TAKEN 0.737 0.716 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.753 NOT TAKEN NOT TAKEN 0.771 NOT TAKEN 0.687 0.710 0.685 NOT TAKEN 0.723 0.656 0.800 0.703 0.737 NOT TAKEN NOT TAKEN 0.677 NOT TAKEN NOT TAKEN 0.730 0.710 NOT TAKEN 0.690 NOT TAKEN NOT TAKEN 0.709 0.696 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.701 NOT TAKEN NOT TAKEN 0.721 NOT TAKEN 0.678 0.688 0.675 NOT TAKEN 0.734 0.483 0.776 0.711 0.728 NOT TAKEN NOT TAKEN 0.618 NOT TAKEN NOT TAKEN 0.722 0.706 NOT TAKEN 0.745 NOT TAKEN NOT TAKEN 0.748 0.753 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.819 NOT TAKEN NOT TAKEN 0.750 NOT TAKEN 0.685 0.722 0.726 NOT TAKEN 0.664 0.407 0.791 0.744 0.765 NOT TAKEN NOT TAKEN 0.631 NOT TAKEN NOT TAKEN 0.663 0.704 NOT TAKEN 0.722 NOT TAKEN NOT TAKEN 0.690 0.688 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.640 NOT TAKEN NOT TAKEN 0.679 NOT TAKEN 0.621 0.712 0.675 NOT TAKEN 0.740 NOT TAKEN 0.805 0.725 0.550 NOT TAKEN NOT TAKEN 0.610 NOT TAKEN NOT TAKEN 0.678 0.711 NOT TAKEN 0.682 NOT TAKEN NOT TAKEN 0.698 0.667 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.115 NOT TAKEN NOT TAKEN 0.654 NOT TAKEN 0.637 0.732 0.641 NOT TAKEN 0.680 NOT TAKEN 0.796 0.757 0.381 NOT TAKEN NOT TAKEN 0.719 NOT TAKEN NOT TAKEN 0.625 0.692 NOT TAKEN 0.682 NOT TAKEN NOT TAKEN 0.711 0.691 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.710 NOT TAKEN 0.653 0.691 0.650 NOT TAKEN 0.617 NOT TAKEN 0.844 0.773 0.196 NOT TAKEN NOT TAKEN 0.658 NOT TAKEN NOT TAKEN NOT TAKEN 0.712 NOT TAKEN 0.649 NOT TAKEN NOT TAKEN 0.683 0.695 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.652 NOT TAKEN 0.637 0.710 0.699 NOT TAKEN NOT TAKEN NOT TAKEN 0.783 0.658 NOT TAKEN NOT TAKEN NOT TAKEN 0.601 NOT TAKEN NOT TAKEN NOT TAKEN 0.695 NOT TAKEN 0.691 NOT TAKEN NOT TAKEN 0.699 0.573 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.680 NOT TAKEN 0.574 0.731 0.695 NOT TAKEN NOT TAKEN NOT TAKEN 0.776 0.564 NOT TAKEN NOT TAKEN NOT TAKEN 1499 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 69 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 5 - 20 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 0.785 0.735 SPILLED NOT TAKEN NOT TAKEN 0.704 0.730 NOT TAKEN 0.678 0.774 0.937 NOT TAKEN NOT TAKEN 0.709 0.744 NOT TAKEN 0.753 0.716 SPILLED NOT TAKEN NOT TAKEN 0.737 0.791 NOT TAKEN 0.673 0.741 0.941 NOT TAKEN NOT TAKEN 0.778 0.788 NOT TAKEN 0.775 0.766 SPILLED NOT TAKEN NOT TAKEN 0.741 0.834 NOT TAKEN 0.720 0.730 SPILLED NOT TAKEN NOT TAKEN 0.734 SPILLED NOT TAKEN NOT TAKEN 0.688 0.944 NOT TAKEN NOT TAKEN 0.714 0.811 NOT TAKEN NOT TAKEN 0.729 0.925 NOT TAKEN NOT TAKEN 0.720 NOT TAKEN NOT TAKEN GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN SPILLED 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN SPILLED 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN SPILLED SPILLED NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN SPILLED 0.000 NOT TAKEN NOT TAKEN SPILLED 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN SPILLED 0.000 NOT TAKEN NOT TAKEN SPILLED 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1500 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 70 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: MALE 6 - 20 PPM C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 0.000 SPILLED NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 SPILLED NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN SPILLED NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 NOT TAKEN NOT TAKEN 0.018 0.020 0.014 0.024 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.017 0.018 0.008 0.022 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.020 0.013 0.011 0.024 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.020 NOT TAKEN 0.020 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN SPILLED 1501 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 71 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 NOT TAKEN 0.028 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.020 0.021 0.019 NOT TAKEN NOT TAKEN 0.023 NOT TAKEN 0.022 0.026 0.018 0.020 0.022 0.023 0.022 0.019 0.018 NOT TAKEN 0.021 0.023 0.023 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.018 0.024 0.021 NOT TAKEN NOT TAKEN 0.028 NOT TAKEN 0.020 0.022 0.018 0.020 0.022 0.022 0.024 0.019 SPILLED NOT TAKEN 0.022 0.025 0.004 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN 0.020 0.022 0.023 NOT TAKEN NOT TAKEN 0.022 NOT TAKEN 0.020 0.025 0.017 0.018 0.020 0.016 0.025 0.020 SPILLED NOT TAKEN 0.023 0.026 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN 0.018 0.017 0.023 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN 0.020 0.023 0.018 0.018 0.022 0.015 0.024 0.019 0.017 NOT TAKEN 0.025 0.022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN 0.018 0.009 0.020 NOT TAKEN NOT TAKEN 0.022 NOT TAKEN 0.021 SPILLED NOT TAKEN 0.018 0.018 NOT TAKEN 0.024 SPILLED 0.014 NOT TAKEN SPILLED 0.021 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.015 NOT TAKEN NOT TAKEN 0.018 NOT TAKEN 0.003 NOT TAKEN NOT TAKEN 0.025 NOT TAKEN 0.019 0.022 NOT TAKEN 0.016 SPILLED NOT TAKEN 0.010 SPILLED SPILLED NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.022 NOT TAKEN 0.018 0.001 NOT TAKEN 0.013 SPILLED NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.022 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1502 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 72 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 2 - 0.5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0.019 0.028 0.021 0.020 0.022 0.026 0.024 0.021 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.022 0.019 0.022 NOT TAKEN 0.021 0.027 0.022 0.019 0.027 0.021 0.020 0.022 0.028 0.021 0.023 NOT TAKEN 0.020 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 0.022 0.020 0.021 NOT TAKEN 0.018 0.022 0.020 0.018 0.027 0.025 0.021 0.023 0.027 0.017 0.023 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.022 0.017 0.024 NOT TAKEN 0.020 NOT TAKEN 0.019 0.016 0.024 0.022 0.020 0.023 0.021 0.018 0.022 NOT TAKEN 0.001 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.017 0.014 0.021 NOT TAKEN 0.018 NOT TAKEN 0.018 0.016 NOT TAKEN 0.010 0.019 0.022 0.024 0.015 0.021 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 0.016 0.012 0.021 NOT TAKEN 0.019 NOT TAKEN 0.019 0.016 NOT TAKEN NOT TAKEN 0.017 0.021 0.020 0.016 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.018 0.007 0.019 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.007 NOT TAKEN NOT TAKEN 0.018 0.016 0.018 0.005 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.013 0.014 NOT TAKEN 0.021 NOT TAKEN 0.020 NOT TAKEN NOT TAKEN 0.010 NOT TAKEN NOT TAKEN 0.012 0.018 0.022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.009 0.015 NOT TAKEN 0.021 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 0.091 0.083 NOT TAKEN 0.087 NOT TAKEN NOT TAKEN 0.085 0.090 0.077 NOT TAKEN 0.014 NOT TAKEN NOT TAKEN 0.068 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1503 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 73 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 NOT TAKEN 0.074 NOT TAKEN 0.077 0.085 0.103 NOT TAKEN 0.081 NOT TAKEN 0.080 NOT TAKEN 0.078 0.079 0.075 0.066 0.046 NOT TAKEN 0.092 SPILLED 0.104 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.077 NOT TAKEN 0.083 0.075 0.113 0.079 0.091 NOT TAKEN 0.076 NOT TAKEN 0.038 0.026 0.089 NOT TAKEN 0.051 NOT TAKEN 0.076 NOT TAKEN 0.071 0.071 0.083 SPILLED 0.040 NOT TAKEN 0.091 0.109 0.094 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.074 NOT TAKEN 0.041 0.076 0.090 0.081 0.078 NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.097 NOT TAKEN 0.103 NOT TAKEN 0.079 NOT TAKEN 0.073 0.060 0.082 NOT TAKEN 0.062 NOT TAKEN 0.097 NOT TAKEN 0.113 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.071 NOT TAKEN 0.074 0.078 0.091 0.061 0.060 NOT TAKEN 0.098 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.105 NOT TAKEN 0.085 NOT TAKEN 0.063 0.028 0.074 NOT TAKEN NOT TAKEN NOT TAKEN 0.088 NOT TAKEN 0.071 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.062 NOT TAKEN NOT TAKEN 0.077 0.100 0.016 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.092 NOT TAKEN NOT TAKEN NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.097 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.061 NOT TAKEN NOT TAKEN 0.076 0.096 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.072 NOT TAKEN NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.071 NOT TAKEN NOT TAKEN NOT TAKEN 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.063 NOT TAKEN NOT TAKEN 0.071 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.056 NOT TAKEN NOT TAKEN NOT TAKEN 0.083 NOT TAKEN NOT TAKEN NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.123 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.057 NOT TAKEN NOT TAKEN 0.064 0.087 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1504 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 74 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 NOT TAKEN 0.076 NOT TAKEN 0.045 NOT TAKEN 0.090 NOT TAKEN 0.076 NOT TAKEN NOT TAKEN 0.073 0.093 0.079 0.076 SPILLED NOT TAKEN NOT TAKEN 0.094 0.074 0.099 NOT TAKEN 0.084 NOT TAKEN 0.068 NOT TAKEN NOT TAKEN NOT TAKEN 0.114 NOT TAKEN 0.072 NOT TAKEN NOT TAKEN 0.067 0.107 0.048 0.078 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.092 NOT TAKEN 0.079 NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.047 NOT TAKEN NOT TAKEN 0.070 0.100 NOT TAKEN 0.064 0.083 NOT TAKEN NOT TAKEN NOT TAKEN 0.068 0.090 NOT TAKEN 0.088 NOT TAKEN 0.062 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.106 NOT TAKEN 0.063 0.070 NOT TAKEN NOT TAKEN NOT TAKEN 0.065 0.076 NOT TAKEN 0.100 NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.055 NOT TAKEN 0.044 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.086 NOT TAKEN 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.084 0.131 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.067 0.087 NOT TAKEN 0.042 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.088 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.009 0.226 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.231 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.211 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.201 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.136 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.113 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1505 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 75 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 0.214 NOT TAKEN 0.226 0.221 SPILLED 0.215 0.244 NOT TAKEN 0.135 SPILLED NOT TAKEN 0.256 0.204 NOT TAKEN NOT TAKEN 0.209 NOT TAKEN 0.182 NOT TAKEN 0.219 0.232 0.196 0.181 0.233 0.203 0.203 0.213 NOT TAKEN NOT TAKEN 0.105 0.199 0.221 NOT TAKEN 0.209 0.239 NOT TAKEN 0.231 0.208 NOT TAKEN 0.241 0.228 NOT TAKEN 0.224 0.184 NOT TAKEN NOT TAKEN 0.223 NOT TAKEN 0.068 NOT TAKEN 0.184 0.227 0.231 0.201 0.255 0.195 0.203 0.227 NOT TAKEN NOT TAKEN 0.143 0.226 0.224 NOT TAKEN 0.210 0.226 NOT TAKEN 0.220 0.217 NOT TAKEN 0.311 0.200 NOT TAKEN 0.251 0.217 NOT TAKEN NOT TAKEN 0.205 NOT TAKEN NOT TAKEN NOT TAKEN 0.219 0.223 0.212 0.211 SPILLED 0.197 0.227 0.248 NOT TAKEN NOT TAKEN 0.240 0.232 0.220 NOT TAKEN 0.211 0.239 NOT TAKEN 0.219 0.207 NOT TAKEN 0.281 0.195 NOT TAKEN 0.237 0.237 NOT TAKEN NOT TAKEN 0.202 NOT TAKEN NOT TAKEN NOT TAKEN 0.138 0.195 0.247 0.199 0.225 0.208 0.209 0.215 NOT TAKEN NOT TAKEN 0.234 0.219 0.141 NOT TAKEN 0.205 0.184 NOT TAKEN 0.234 0.214 NOT TAKEN SPILLED 0.194 NOT TAKEN 0.228 0.237 NOT TAKEN NOT TAKEN 0.205 NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.182 0.243 0.175 0.189 0.197 0.183 0.193 NOT TAKEN NOT TAKEN NOT TAKEN 0.231 0.171 NOT TAKEN 0.189 0.199 NOT TAKEN 0.234 0.210 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.239 0.246 NOT TAKEN NOT TAKEN 0.200 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.176 0.243 0.192 SPILLED 0.201 0.044 0.192 NOT TAKEN NOT TAKEN NOT TAKEN 0.204 NOT TAKEN NOT TAKEN 0.189 0.152 NOT TAKEN 0.229 0.207 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.173 0.157 NOT TAKEN NOT TAKEN 0.197 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.164 0.249 0.204 SPILLED SPILLED NOT TAKEN 0.177 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.182 0.199 NOT TAKEN 0.203 0.167 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.212 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.179 0.245 0.183 0.265 0.207 NOT TAKEN 0.224 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1506 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 76 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.242 0.224 0.234 0.154 0.132 0.221 0.180 0.205 NOT TAKEN 0.232 0.196 0.209 0.216 0.218 0.230 0.224 0.006 0.221 0.212 0.184 NOT TAKEN NOT TAKEN 0.205 NOT TAKEN 0.227 0.209 NOT TAKEN NOT TAKEN 0.203 0.211 0.234 NOT TAKEN 0.211 0.195 0.183 0.217 0.182 0.254 0.223 0.267 SPILLED 0.202 0.228 NOT TAKEN NOT TAKEN 0.226 NOT TAKEN 0.236 0.229 NOT TAKEN NOT TAKEN 0.194 0.206 0.210 NOT TAKEN 0.226 0.205 0.182 0.221 0.200 0.265 0.169 NOT TAKEN 0.125 0.202 0.223 NOT TAKEN NOT TAKEN 0.193 NOT TAKEN 0.240 0.215 NOT TAKEN NOT TAKEN 0.218 0.167 0.169 NOT TAKEN 0.230 0.182 0.148 0.208 0.192 0.254 0.192 NOT TAKEN SPILLED 0.195 0.217 NOT TAKEN NOT TAKEN 0.119 NOT TAKEN 0.118 0.209 NOT TAKEN NOT TAKEN 0.213 0.191 0.244 NOT TAKEN 0.243 0.198 0.139 NOT TAKEN 0.212 SPILLED 0.183 NOT TAKEN NOT TAKEN 0.175 0.158 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.206 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.186 NOT TAKEN 0.196 0.180 NOT TAKEN NOT TAKEN 0.186 SPILLED 0.075 NOT TAKEN NOT TAKEN NOT TAKEN 0.205 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.198 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.180 NOT TAKEN NOT TAKEN 0.173 NOT TAKEN NOT TAKEN 0.194 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.201 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.143 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.028 NOT TAKEN NOT TAKEN 0.181 NOT TAKEN NOT TAKEN 0.211 0.286 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.201 NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 NOT TAKEN NOT TAKEN 0.872 NOT TAKEN 1.001 NOT TAKEN NOT TAKEN 1.059 NOT TAKEN 0.923 NOT TAKEN NOT TAKEN 0.898 NOT TAKEN 0.930 NOT TAKEN NOT TAKEN 0.957 NOT TAKEN 0.868 NOT TAKEN NOT TAKEN 0.938 NOT TAKEN 0.865 NOT TAKEN NOT TAKEN 0.716 NOT TAKEN 0.853 NOT TAKEN NOT TAKEN 0.836 NOT TAKEN 0.692 NOT TAKEN NOT TAKEN 0.849 NOT TAKEN 0.662 1507 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 77 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 NOT TAKEN NOT TAKEN 1.075 1.229 NOT TAKEN 0.979 0.838 NOT TAKEN 1.024 1.058 NOT TAKEN NOT TAKEN 0.956 1.118 NOT TAKEN 0.852 0.858 0.993 NOT TAKEN SPILLED NOT TAKEN 0.945 NOT TAKEN 0.976 0.935 0.885 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.224 NOT TAKEN NOT TAKEN 0.904 1.363 NOT TAKEN NOT TAKEN 1.035 NOT TAKEN 1.047 1.148 NOT TAKEN NOT TAKEN 1.068 1.127 NOT TAKEN 0.727 0.805 0.916 NOT TAKEN SPILLED NOT TAKEN 1.140 NOT TAKEN 1.081 0.903 0.994 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.208 NOT TAKEN NOT TAKEN SPILLED 1.305 NOT TAKEN NOT TAKEN 1.052 NOT TAKEN 1.044 1.178 NOT TAKEN NOT TAKEN 1.022 1.134 NOT TAKEN 0.958 0.831 0.928 NOT TAKEN SPILLED NOT TAKEN 1.026 NOT TAKEN 1.041 0.913 0.941 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.207 NOT TAKEN NOT TAKEN 1.309 1.268 NOT TAKEN NOT TAKEN 0.788 NOT TAKEN 1.408 1.033 NOT TAKEN NOT TAKEN 1.000 0.953 NOT TAKEN 0.867 0.836 0.871 NOT TAKEN SPILLED NOT TAKEN 0.830 NOT TAKEN SPILLED 0.904 0.863 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.194 NOT TAKEN NOT TAKEN 1.213 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.121 1.108 NOT TAKEN NOT TAKEN 0.958 0.693 NOT TAKEN 0.473 0.698 0.846 NOT TAKEN SPILLED NOT TAKEN 0.926 NOT TAKEN 0.953 0.912 0.877 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.071 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.161 1.093 NOT TAKEN NOT TAKEN 0.919 0.542 NOT TAKEN NOT TAKEN 0.835 0.919 NOT TAKEN SPILLED NOT TAKEN 0.614 NOT TAKEN 1.057 0.879 0.714 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.003 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.312 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.291 0.899 NOT TAKEN SPILLED NOT TAKEN 0.884 NOT TAKEN 1.036 0.852 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.054 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.178 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.901 NOT TAKEN 0.559 NOT TAKEN 0.758 NOT TAKEN 1.171 0.700 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.846 1508 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 78 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 NOT TAKEN 0.977 0.884 1.177 1.117 NOT TAKEN 1.158 NOT TAKEN NOT TAKEN 0.464 0.896 0.757 NOT TAKEN NOT TAKEN 0.938 0.981 NOT TAKEN 1.198 0.955 0.845 NOT TAKEN 1.006 NOT TAKEN SPILLED NOT TAKEN 0.770 0.979 0.986 1.065 0.826 1.190 NOT TAKEN 1.048 0.917 1.231 1.123 NOT TAKEN 1.040 NOT TAKEN NOT TAKEN 1.602 0.868 0.909 NOT TAKEN NOT TAKEN 0.692 0.952 NOT TAKEN 1.080 0.945 0.768 NOT TAKEN 1.002 NOT TAKEN 1.275 NOT TAKEN NOT TAKEN 0.889 1.027 0.952 0.736 1.366 NOT TAKEN 1.037 0.917 1.082 1.072 NOT TAKEN 1.145 NOT TAKEN NOT TAKEN 1.161 0.893 0.936 NOT TAKEN NOT TAKEN 0.951 0.983 NOT TAKEN 1.122 0.927 0.916 NOT TAKEN 1.048 NOT TAKEN 1.058 NOT TAKEN NOT TAKEN 0.805 SPILLED 0.985 0.567 1.146 NOT TAKEN 0.982 0.985 1.182 1.013 NOT TAKEN 1.108 NOT TAKEN NOT TAKEN NOT TAKEN 0.850 0.916 NOT TAKEN NOT TAKEN SPILLED 0.923 NOT TAKEN 1.073 0.795 0.754 NOT TAKEN 1.286 NOT TAKEN 0.756 NOT TAKEN NOT TAKEN 1.088 0.862 1.026 NOT TAKEN 0.980 NOT TAKEN NOT TAKEN 0.830 NOT TAKEN 1.049 NOT TAKEN 0.965 NOT TAKEN NOT TAKEN NOT TAKEN 0.805 0.863 NOT TAKEN NOT TAKEN 0.897 0.913 NOT TAKEN 1.168 0.897 0.822 NOT TAKEN 0.944 NOT TAKEN 0.741 NOT TAKEN NOT TAKEN 0.933 0.868 NOT TAKEN NOT TAKEN 0.942 NOT TAKEN NOT TAKEN 0.945 NOT TAKEN 0.876 NOT TAKEN 1.223 NOT TAKEN NOT TAKEN NOT TAKEN 0.820 0.901 NOT TAKEN NOT TAKEN 0.875 0.844 NOT TAKEN 1.190 0.951 0.783 NOT TAKEN 0.898 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.794 SPILLED NOT TAKEN NOT TAKEN 1.040 NOT TAKEN NOT TAKEN 0.394 NOT TAKEN 0.948 NOT TAKEN 0.845 NOT TAKEN NOT TAKEN NOT TAKEN 0.753 0.925 NOT TAKEN NOT TAKEN 0.880 0.791 NOT TAKEN 0.907 0.822 0.824 NOT TAKEN 0.842 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.562 0.870 NOT TAKEN NOT TAKEN 1.100 NOT TAKEN NOT TAKEN 0.773 NOT TAKEN 0.784 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.761 0.859 NOT TAKEN NOT TAKEN 0.870 SPILLED NOT TAKEN 0.988 0.782 0.775 NOT TAKEN 0.911 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.866 NOT TAKEN NOT TAKEN 1.195 1509 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 79 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 5 - 20 PPM C93178 C93179 C93180 0.863 1.005 NOT TAKEN 0.841 1.049 NOT TAKEN 0.901 0.995 NOT TAKEN 0.864 1.033 NOT TAKEN 0.825 1.062 NOT TAKEN 0.846 1.068 NOT TAKEN 0.749 0.831 NOT TAKEN 0.870 1.202 NOT TAKEN GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 NOT TAKEN 0.000 SPILLED 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN SPILLED 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN SPILLED 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 SPILLED NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 SPILLED NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 SPILLED 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 1510 Appendix 4 Covance 6329-183 3M T-6295 Individual Test Material Consumption Data (mg/kg/day) PAGE: 80 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK NUMBER 76 80 84 88 92 96 100 104 ------------------------------------------------------------------------------------------------------------------------------------ GROUP: FEMALE 6 - 20 PPM C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN NOT TAKEN 0.000 NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN NOT TAKEN SPILLED SPILLED 0.000 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 SPILLED 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 NOT TAKEN NOT TAKEN NOT TAKEN 0.000 0.000 0.000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN NOT TAKEN 0.000 0.000 1511 APPENDIX 5 Individual Clinical Hematology Data Individual Clinical Chemistry Data Individual Clinical Urinalysis Data Individual Urine Chemistry Data Covance 6329-183 3M T-6295 1512 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 6.96 7.13 8.05 7.86 7.61 7.22 7.59 8.09 6.73 7.48 14.5 15.1 16.4 16.2 15.1 14.2 15.1 16.1 14.5 14.4 42.0 44.7 47.6 47.4 45.3 41.9 45.1 48.0 42.1 43.8 60.3 62.7 59.1 60.4 59.5 58.0 59.4 59.2 62.5 58.6 20.8 21.2 20.4 20.6 19.9 19.6 19.9 19.9 21.6 19.3 34.6 33.7 34.6 34.1 33.4 33.8 33.4 33.6 34.6 32.9 1086 1208 1021 941 1057 909 1181 914 1022 1145 MEAN S.D. N 7.47 .459 10 15.2 .81 10 44.8 2.35 10 60.0 1.56 10 20.3 .73 10 33.9 .59 10 1048 107.7 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 7.14 8.02 6.58 7.14 7.45 6.80 7.19 7.65 6.97 7.22 14.5 15.9 14.0 13.8 14.9 13.9 15.6 15.6 14.2 14.8 42.5 47.6 40.8 41.4 44.1 40.7 46.0 45.8 41.6 43.9 59.5 59.4 62.1 58.0 59.2 59.8 64.0 59.9 59.7 60.7 20.4 19.9 21.3 19.3 19.9 20.5 21.6 20.4 20.3 20.5 34.2 33.4 34.4 33.3 33.7 34.2 33.8 34.0 34.1 33.7 993 1028 1349 1138 1096 1033 1071 952 1022 1139 MEAN S.D. N 7.22 .414 10 14.7 .77 10 43.4 2.43 10 60.2 1.69 10 20.4 .67 10 33.9 .36 10 1082 111.4 10 Covance 6329-183 3M T-6295 1513 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 6.27 8.26 8.07 7.80 7.21 7.93 7.59 7.82 7.57 6.90 13.6 17.3 16.2 16.5 14.5 15.6 15.0 15.3 14.7 14.7 39.3 50.4 48.4 48.1 42.6 46.8 43.8 46.0 43.9 42.1 62.7 61.1 60.0 61.6 59.1 59.1 57.7 58.8 58.0 61.1 21.7 20.9 20.1 21.2 20.1 19.6 19.8 19.5 19.4 21.4 34.6 34.2 33.5 34.3 34.1 33.3 34.3 33.1 33.5 35.0 942 1075 1006 974 1048 1011 1040 968 1186 1033 MEAN S.D. N 7.54 .599 10 15.3 1.09 10 45.1 3.39 10 59.9 1.65 10 20.4 .85 10 34.0 .61 10 1028 68.7 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 7.39 8.70 6.87 7.81 6.91 7.89 6.93 7.39 7.71 6.66 15.0 16.6 14.4 16.4 14.7 16.3 14.3 16.0 16.2 13.8 43.8 49.2 40.9 47.9 43.2 48.0 41.9 46.9 48.0 40.5 59.3 56.5 59.5 61.3 62.5 60.8 60.5 63.5 62.3 60.7 20.3 19.1 20.9 21.0 21.2 20.7 20.7 21.7 21.0 20.8 34.2 33.8 35.2 34.2 34.0 34.0 34.1 34.1 33.7 34.2 998 1095 1043 956 994 794 1092 1076 1208 875 MEAN S.D. N 7.43 .622 10 15.4 1.04 10 45.0 3.32 10 60.7 1.97 10 20.7 .68 10 34.1 .41 10 1013 118.6 10 Covance 6329-183 3M T-6295 1514 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 7.83 7.17 7.46 7.01 7.22 7.11 7.47 7.26 7.24 7.21 15.6 15.0 15.6 14.2 14.9 15.0 15.1 14.6 14.7 15.3 46.7 44.4 46.4 41.6 43.5 44.8 44.6 42.7 43.4 44.1 59.6 61.9 62.1 59.4 60.3 63.1 59.7 58.8 59.9 61.2 19.9 20.9 20.9 20.2 20.6 21.1 20.3 20.1 20.3 21.2 33.5 33.8 33.6 34.1 34.2 33.4 33.9 34.2 33.9 34.6 995 935 1224 1120 831 1051 1145 1170 999 1044 MEAN S.D. N 7.30 .234 10 15.0 .44 10 44.2 1.56 10 60.6 1.40 10 20.6 .45 10 33.9 .37 10 1051 118.1 10 Covance 6329-183 3M T-6295 1515 ANIMAL NUMBER ------ Group: 1 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 MEAN S.D. N Group: 2 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 10.7 .6 9.2 .7 .1 .0 6 86 6 1 0 10.7 1.2 8.8 .6 .0 .0 12 82 5 0 0 8.4 .8 7.0 .5 .1 .0 9 84 5 1 0 12.0 .6 10.7 .6 .1 .0 5 89 5 1 0 8.8 .6 7.6 .5 .1 .0 7 86 6 1 0 6.6 .5 5.4 .6 .1 .0 8 82 9 1 0 7.1 .9 5.7 .4 .0 .0 13 81 6 1 0 5.7 .2 5.1 .3 .1 .0 4 89 6 1 0 9.0 1.0 7.2 .6 .1 .0 11 80 7 1 0 7.2 1.0 5.1 .9 .2 .0 14 71 12 2 0 8.6 .7 7.2 .6 .1 .0 9 83 7 1 0 2.03 .30 1.92 .16 .06 .00 3.5 5.3 2.2 .5 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 7.8 .6 6.6 .5 .1 .0 8 85 6 1 0 7.1 .5 6.3 .2 .0 .0 8 90 2 1 0 15.1 4.9 8.8 1.2 .1 .0 33 58 8 1 0 9.8 1.1 7.7 .8 .1 .0 11 79 9 1 0 10.7 1.3 8.6 .7 .1 .0 12 80 7 1 0 8.2 .9 6.4 .7 .2 .0 11 78 9 2 0 7.2 .6 6.2 .3 .0 .0 9 86 4 1 0 8.5 .6 7.5 .3 .0 .0 8 88 3 1 0 11.2 .8 9.7 .6 .1 .0 8 86 5 1 0 7.0 .7 6.0 .3 .1 .0 9 85 4 1 0 9.3 1.2 7.4 .6 .1 .0 12 82 6 1 0 2.54 1.32 1.29 .31 .06 .00 7.6 9.1 2.5 .3 .0 10 10 10 10 10 10 10 10 10 10 10 1516 ANIMAL NUMBER ------ Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 MEAN S.D. N Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 12.9 1.0 10.7 1.0 .1 .0 8 83 8 1 0 9.1 .5 8.0 .5 .1 .0 6 88 6 1 0 5.6 .5 4.8 .2 .0 .0 8 86 4 1 0 7.7 .3 6.8 .4 .1 .0 4 90 5 1 0 11.8 1.0 10.1 .6 .1 .0 8 86 5 1 0 8.2 .5 7.4 .2 .1 .0 7 90 3 1 0 9.4 .7 7.9 .7 .0 .0 8 84 7 0 0 7.2 .6 5.7 .8 .1 .0 9 79 11 1 0 9.6 .7 7.9 .8 .2 .0 7 83 8 2 0 11.8 .8 10.2 .7 .1 .0 6 86 6 1 0 9.3 .7 8.0 .6 .1 .0 7 86 6 1 0 2.30 .23 1.94 .26 .06 .00 1.4 3.4 2.3 .5 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 7.6 .8 5.3 .9 .5 .0 11 70 12 7 0 5.8 .4 5.1 .2 .1 .0 6 88 4 2 0 6.8 .6 5.6 .5 .1 .0 9 82 7 1 0 7.0 .5 6.3 .2 .0 .0 6 90 3 0 0 9.3 1.0 7.4 .7 .1 .0 11 80 8 1 0 5.6 .3 4.9 .3 .0 .0 5 89 5 1 0 8.2 .7 7.1 .3 .1 .0 8 87 4 1 0 7.7 .4 7.0 .3 .0 .0 5 90 4 1 0 7.9 .5 7.1 .2 .0 .0 7 90 3 0 0 6.3 .6 5.1 .5 .1 .0 10 81 8 1 0 7.2 .6 6.1 .4 .1 .0 8 85 6 2 0 1.15 .21 .99 .24 .15 .00 2.3 6.5 2.9 2.0 .0 10 10 10 10 10 10 10 10 10 10 10 1517 ANIMAL NUMBER ------ Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 5.6 .6 4.6 .4 .0 .0 11 82 7 1 0 6.7 .8 5.8 .1 .0 .0 12 86 2 1 0 3.9 .4 3.2 .2 .0 .0 10 83 6 1 0 12.1 1.8 8.6 1.4 .2 .0 15 72 11 2 0 6.5 .4 5.8 .2 .1 .0 7 89 4 1 0 7.3 .4 6.3 .5 .1 .0 6 87 6 1 0 7.8 .9 6.2 .6 .1 .0 12 79 7 2 0 11.4 1.4 9.3 .5 .2 .0 12 82 4 1 0 10.9 .9 9.0 .8 .1 .0 8 83 7 1 0 11.4 .9 9.7 .8 .1 .0 8 85 7 1 0 8.4 .8 6.8 .6 .1 .0 10 83 6 1 0 2.87 .46 2.19 .38 .07 .00 2.8 4.8 2.4 .4 .0 10 10 10 10 10 10 10 10 10 10 10 1518 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 - - - - - C92506 - - - - - C92524 - - - - - C92526 - - - - - C92529 - - - - - C92542 - - - - - C92543 - - - - - C92546 - - - - - C92548 - - - - - C92553 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 - - - - - C92574 - - - - - C92575 - - - - - C92585 - - - - - C92586 - - - - - C92587 - - - - - C92610 - - - - - C92613 - - - - - C92615 - - - - - C92618 - - - - - Covance 6329-183 3M T-6295 1519 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 - - - - - C92646 - - - - - C92650 - - - - - C92652 - - - - - C92657 - - - - - C92668 - - - - - C92676 - - - - - C92678 - - - - - C92684 - - - - - C92686 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 - - - - - C92715 - - - - - C92716 - - - - - C92718 - - - - - C92725 - - - - - C92731 - - - - - C92732 - - - - - C92734 - - - - - C92744 - - - - - C92746 - - - - - Covance 6329-183 3M T-6295 1520 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 - - - - - C92759 - - - - - C92779 - - - - - C92784 - - - - - C92793 - - - - - C92798 - - - - - C92799 - - - - - C92805 - - - - - C92807 - - - - - C92811 - - - - - Covance 6329-183 3M T-6295 1521 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 7.48 6.77 7.39 7.18 7.13 7.26 7.62 6.97 7.43 7.61 15.5 14.4 15.7 15.4 15.2 15.0 15.2 15.0 14.6 15.8 46.1 42.8 46.4 45.5 45.3 45.2 44.2 44.1 44.4 46.7 61.6 63.2 62.9 63.4 63.6 62.3 58.1 63.3 59.8 61.4 20.7 21.3 21.2 21.4 21.3 20.7 19.9 21.5 19.7 20.7 33.6 33.8 33.8 33.8 33.5 33.1 34.3 34.0 32.9 33.8 1199 983 1058 874 1146 1222 1083 1208 1103 1153 MEAN S.D. N 7.28 .276 10 15.2 .45 10 45.1 1.20 10 62.0 1.80 10 20.8 .63 10 33.7 .41 10 1103 109.6 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 6.97 7.38 7.78 6.99 6.94 7.48 7.32 7.36 7.74 8.01 14.6 15.2 16.3 14.6 14.6 15.9 15.0 15.8 15.4 15.8 43.6 45.0 47.7 44.1 43.9 46.7 45.6 46.0 46.9 47.1 62.5 60.9 61.3 63.1 63.2 62.4 62.4 62.4 60.6 58.8 20.9 20.6 20.9 20.9 21.0 21.3 20.6 21.5 19.8 19.7 33.4 33.9 34.1 33.1 33.3 34.1 32.9 34.4 32.7 33.5 1182 1595 1061 1095 983 955 1106 1187 1069 1023 MEAN S.D. N 7.40 .367 10 15.3 .62 10 45.7 1.46 10 61.8 1.36 10 20.7 .58 10 33.5 .57 10 1126 181.3 10 Covance 6329-183 3M T-6295 1522 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 7.25 7.84 7.43 7.72 7.82 7.65 7.76 6.79 6.99 7.66 14.8 16.0 14.8 15.1 15.4 16.2 15.1 14.6 13.8 15.6 44.5 46.6 45.4 45.6 45.5 47.4 45.4 43.0 42.1 46.7 61.3 59.4 61.2 59.1 58.2 61.9 58.5 63.3 60.2 60.9 20.3 20.4 20.0 19.6 19.7 21.1 19.5 21.4 19.7 20.4 33.2 34.3 32.7 33.1 33.8 34.1 33.2 33.9 32.8 33.5 973 1189 1096 1273 951 1127 1175 856 1068 979 MEAN S.D. N 7.49 .367 10 15.1 .70 10 45.2 1.64 10 60.4 1.62 10 20.2 .64 10 33.5 .55 10 1069 128.2 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 8.08 7.30 7.37 6.98 8.01 7.00 7.69 7.33 7.21 7.08 15.7 15.8 15.5 15.3 16.2 15.0 15.5 15.2 14.8 15.1 47.2 45.8 46.5 45.8 47.2 44.7 46.1 45.5 45.0 44.2 58.4 62.8 63.1 65.7 58.9 63.8 60.0 62.0 62.4 62.4 19.4 21.6 21.1 22.0 20.2 21.4 20.1 20.8 20.5 21.3 33.2 34.5 33.3 33.5 34.3 33.5 33.6 33.5 32.8 34.1 1324 1085 1046 893 1091 1023 1211 1115 1181 1066 MEAN S.D. N 7.40 .396 10 15.4 .42 10 45.8 1.00 10 62.0 2.25 10 20.8 .79 10 33.6 .52 10 1104 116.6 10 Covance 6329-183 3M T-6295 1523 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 7.27 7.19 7.54 7.36 7.57 7.13 6.97 7.38 7.68 7.66 15.2 15.1 15.0 15.6 15.3 15.6 15.0 15.3 15.3 15.8 44.5 45.2 45.5 46.3 46.4 45.3 44.0 45.3 46.1 47.8 61.3 62.9 60.4 62.9 61.3 63.5 63.1 61.5 60.0 62.5 21.0 21.0 19.9 21.3 20.2 21.8 21.5 20.7 20.0 20.7 34.2 33.3 32.9 33.8 33.0 34.4 34.1 33.7 33.3 33.1 1171 1092 1015 1155 1006 1022 1193 1042 1269 1112 MEAN S.D. N 7.38 .238 10 15.3 .27 10 45.6 1.07 10 61.9 1.21 10 20.8 .64 10 33.6 .53 10 1108 88.4 10 Covance 6329-183 3M T-6295 1524 ANIMAL NUMBER ------ Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 MEAN S.D. N Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 7.6 .7 6.5 .3 .1 .0 9 86 4 1 0 6.6 .5 5.9 .1 .1 .0 8 89 1 1 0 4.6 .7 3.6 .1 .2 .0 15 77 3 5 0 6.4 .9 5.2 .3 .0 .0 14 81 5 1 0 4.5 .5 3.7 .2 .1 .0 11 82 5 2 0 7.7 .8 6.2 .6 .1 .0 11 80 8 1 0 8.9 .8 7.8 .2 .0 .0 9 88 3 0 0 11.7 1.0 10.2 .5 .1 .0 8 87 4 1 0 9.9 .4 8.8 .6 .1 .0 4 88 6 1 0 6.2 .3 5.2 .5 .1 .0 5 84 8 2 0 7.4 .7 6.3 .3 .1 .0 9 84 5 2 0 2.27 .23 2.12 .20 .06 .00 3.5 4.0 2.2 1.4 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 5.5 1.1 4.2 .2 .0 .0 20 76 3 1 0 8.1 .6 7.1 .3 .1 .0 8 87 3 2 0 7.3 2.8 4.3 .2 .0 .0 38 58 3 1 0 6.1 .3 5.7 .1 .1 .0 4 94 1 1 0 5.6 .2 5.1 .2 .0 .0 4 91 4 1 0 7.8 .3 6.9 .5 .1 .0 4 89 6 1 0 6.7 .4 5.8 .4 .1 .0 6 86 6 1 0 6.2 .4 5.5 .2 .0 .0 7 89 3 0 0 6.9 .4 5.9 .4 .1 .0 6 85 6 2 0 5.1 .4 4.5 .1 .0 .0 7 89 3 1 0 6.5 .7 5.5 .3 .0 .0 10 84 4 1 0 1.01 .78 1.01 .13 .05 .00 10.8 10.4 1.7 .6 .0 10 10 10 10 10 10 10 10 10 10 10 1525 ANIMAL NUMBER ------ Group: 3 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 MEAN S.D. N Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 6.1 .7 5.0 .4 .0 .0 11 82 6 0 0 8.2 .2 7.7 .3 .1 .0 2 93 3 1 0 3.4 .5 2.8 .1 .0 .0 14 82 3 1 0 2.3 .4 1.6 .3 .0 .0 16 69 14 1 0 4.4 .2 4.1 .1 .0 .0 4 93 2 1 0 7.0 .4 6.3 .2 .0 .0 5 90 3 1 0 4.4 .5 3.4 .3 .1 .0 12 78 7 2 0 6.0 .3 5.4 .3 .1 .0 5 90 4 1 0 3.6 .5 2.8 .2 .1 .0 15 77 6 2 0 4.5 .4 4.0 .1 .0 .0 8 88 3 1 0 5.0 .4 4.3 .2 .0 .0 9 84 5 1 0 1.80 .15 1.83 .11 .05 .00 5.1 7.9 3.5 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 5.0 .6 4.0 .2 .1 .0 12 81 4 2 0 4.8 .4 4.2 .1 .0 .0 8 88 3 1 0 5.1 .4 4.5 .1 .1 .0 7 88 3 2 0 13.0 1.0 11.5 .4 .1 .0 8 88 3 1 0 6.1 .3 5.6 .2 .0 .0 6 91 2 0 0 3.4 .3 2.7 .3 .1 .0 9 80 8 2 0 7.9 .5 7.0 .4 .1 .0 6 88 5 1 0 9.2 .8 8.0 .3 .1 .0 9 86 3 1 0 5.3 .3 4.7 .2 .1 .0 6 90 4 1 0 9.0 .5 8.1 .3 .1 .0 5 90 4 1 0 6.9 .5 6.0 .2 .1 .0 8 87 4 1 0 2.88 .23 2.62 .11 .04 .00 2.1 3.7 1.7 .6 .0 10 10 10 10 10 10 10 10 10 10 10 1526 ANIMAL NUMBER ------ Group: 5 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 6.7 .3 6.2 .1 .0 .0 5 93 2 0 0 4.6 .5 3.8 .2 .1 .0 10 84 4 1 0 6.6 .7 5.7 .2 .1 .0 10 86 2 1 0 10.9 .6 9.8 .4 .1 .0 6 90 4 1 0 6.1 .5 5.2 .4 .1 .0 7 85 6 1 0 4.8 .5 4.0 .2 .1 .0 10 84 4 2 0 5.8 .7 4.4 .5 .1 .0 12 76 9 2 0 5.3 .3 4.8 .2 .0 .0 5 90 4 1 0 6.5 .7 5.6 .1 .1 .0 10 86 2 2 0 5.2 .3 4.6 .2 .1 .0 5 88 4 2 0 6.2 .5 5.4 .2 .1 .0 8 86 4 1 0 1.80 .17 1.72 .14 .04 .00 2.7 4.6 2.1 .7 .0 10 10 10 10 10 10 10 10 10 10 10 1527 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 - - - - - C92864 - - - - - C92876 - - - - - C92877 - - - - - C92882 - - - - - C92883 - - - - - C92914 - - - - - C92919 - - - - - C92925 - - - - - C92926 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 - - - - - C92945 - - - - - C92950 - - - - - C92953 - - - - - C92959 - - - - - C92964 - - - - - C92968 - - - - - C92969 - - - - - C92976 - - - - - C92982 - - - - - Covance 6329-183 3M T-6295 1528 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 - - - - - C92996 - - - - - C92998 - - - - - C93004 - - - - - C93006 - - - - - C93008 - - - - - C93023 - - - - - C93031 - - - - - C93033 - - - - - C93045 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 - - - - - C93067 - - - - - C93070 - - - - - C93074 - - - - - C93075 - - - - - C93083 - - - - - C93084 - - - - - C93089 - - - - - C93094 - - - - - C93105 - - - - - Covance 6329-183 3M T-6295 1529 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 - - - - - C93123 - - - - - C93142 - - - - - C93144 - - - - - C93151 - - - - - C93155 - - - - - C93158 - - - - - C93159 - - - - - C93164 - - - - - C93175 - - - - - Covance 6329-183 3M T-6295 1530 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 8.63 8.60 8.93 8.84 7.98 8.39 9.27 8.69 7.74 8.46 15.1 15.9 16.7 15.8 15.4 15.3 16.7 15.3 14.5 15.8 45.7 46.3 48.0 45.7 45.2 44.7 48.5 45.5 42.0 47.1 53.0 53.8 53.7 51.7 56.6 53.3 52.3 52.4 54.3 55.6 17.5 18.5 18.7 17.9 19.3 18.2 18.0 17.6 18.7 18.6 33.1 34.4 34.7 34.6 34.2 34.1 34.5 33.5 34.4 33.5 940 1103 1188 1157 1078 1533 1165 1173 1147 940 MEAN S.D. N 8.55 .446 10 15.6 .69 10 45.9 1.83 10 53.7 1.52 10 18.3 .56 10 34.1 .55 10 1142 164.6 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 8.56 9.75 8.77 8.90 8.69 8.87 8.09 8.98 8.80 8.32 15.4 17.1 15.4 16.4 15.5 15.9 15.5 15.8 15.9 15.3 46.7 49.8 46.2 48.4 45.3 46.5 46.0 47.2 45.9 45.0 54.5 51.1 52.6 54.4 52.1 52.4 56.9 52.6 52.2 54.1 18.0 17.6 17.6 18.5 17.8 17.9 19.1 17.6 18.1 18.3 32.9 34.4 33.4 33.9 34.2 34.2 33.6 33.5 34.7 33.9 1088 1141 1143 1140 1156 1135 1093 907 1218 904 MEAN S.D. N 8.77 .441 10 15.8 .56 10 46.7 1.45 10 53.3 1.68 10 18.0 .48 10 33.9 .53 10 1092 104.7 10 Covance 6329-183 3M T-6295 1531 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 8.63 9.12 7.99 9.01 8.78 8.30 8.79 9.04 7.97 9.22 16.0 15.9 14.6 16.7 15.7 15.8 15.8 15.8 14.8 15.8 47.2 46.4 43.4 48.6 47.7 47.7 46.8 46.9 43.9 46.1 54.6 50.9 54.3 54.0 54.4 57.5 53.2 51.9 55.1 50.0 18.5 17.4 18.2 18.5 17.9 19.0 18.0 17.5 18.6 17.1 33.9 34.3 33.5 34.3 33.0 33.0 33.8 33.7 33.7 34.2 1090 1165 1156 1507 1422 905 1111 1161 922 1239 MEAN S.D. N 8.68 .456 10 15.7 .60 10 46.5 1.65 10 53.6 2.19 10 18.1 .60 10 33.7 .47 10 1168 189.6 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 8.48 9.13 9.14 7.64 8.20 8.76 8.74 8.82 8.71 8.06 14.9 16.3 15.8 14.0 15.2 15.1 15.3 15.8 15.2 14.8 44.9 47.5 46.1 41.4 44.8 44.6 46.2 46.2 45.3 44.9 53.0 52.1 50.5 54.3 54.7 51.0 52.8 52.4 52.0 55.7 17.6 17.9 17.3 18.3 18.6 17.3 17.5 17.9 17.4 18.4 33.3 34.4 34.2 33.7 34.0 33.9 33.1 34.2 33.5 33.0 1091 1319 1025 1062 1161 1146 885 1279 839 977 MEAN S.D. N 8.57 .478 10 15.2 .63 10 45.2 1.61 10 52.8 1.64 10 17.8 .48 10 33.7 .49 10 1078 155.6 10 Covance 6329-183 3M T-6295 1532 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 7.96 8.28 8.11 7.96 8.09 8.21 8.67 8.32 8.30 8.41 15.1 14.9 14.8 14.7 15.1 14.3 15.9 15.5 14.7 15.8 45.1 44.2 43.8 44.1 43.9 42.8 46.5 45.2 43.4 46.8 56.6 53.4 54.0 55.5 54.2 52.1 53.7 54.4 52.4 55.6 19.0 18.0 18.3 18.5 18.7 17.4 18.4 18.7 17.7 18.8 33.5 33.7 33.8 33.4 34.5 33.5 34.3 34.3 33.8 33.8 1098 1287 941 1000 1061 1124 1139 1328 1261 1147 MEAN S.D. N 8.23 .216 10 15.1 .51 10 44.6 1.30 10 54.2 1.42 10 18.4 .51 10 33.9 .38 10 1139 124.3 10 Covance 6329-183 3M T-6295 1533 ANIMAL NUMBER ------ Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 MEAN S.D. N Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 6.2 .7 4.9 .5 .1 .0 12 79 8 1 0 7.0 .8 5.4 .7 .1 .0 12 77 9 1 0 7.2 .7 5.5 .8 .1 .0 10 76 12 2 0 5.1 .8 3.6 .5 .1 .0 17 71 10 2 0 9.2 1.1 6.9 1.0 .2 .0 12 75 11 2 0 11.8 1.3 9.0 1.2 .2 .1 11 76 10 2 0 9.4 1.6 7.0 .7 .2 .0 17 74 7 2 0 7.6 1.3 5.5 .6 .2 .0 17 72 8 2 0 9.1 1.0 6.6 1.2 .2 .0 11 73 14 2 0 9.4 1.7 6.4 1.0 .3 .0 18 68 10 3 0 8.2 1.1 6.1 .8 .2 .0 14 74 10 2 0 1.95 .37 1.46 .27 .07 .03 3.1 3.2 2.1 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 7.9 1.5 5.3 .9 .1 .0 19 68 11 2 0 7.7 .7 6.3 .5 .1 .0 9 83 7 1 0 10.0 1.3 7.3 1.1 .3 .0 13 73 11 3 0 9.0 1.4 6.7 .9 .0 .0 15 74 10 0 0 10.2 1.0 7.9 1.0 .2 .0 10 78 10 2 0 6.9 1.2 4.8 .7 .1 .0 18 71 10 2 0 7.8 1.1 5.7 .7 .3 .0 14 73 9 4 0 9.8 1.6 7.0 1.0 .2 .0 16 71 10 2 0 8.7 1.6 6.3 .6 .1 .0 19 72 7 1 0 7.2 1.3 4.9 .9 .1 .0 18 68 12 2 0 8.5 1.3 6.2 .8 .2 .0 15 73 10 2 0 1.20 .28 1.04 .19 .10 .00 3.6 4.5 1.6 1.1 .0 10 10 10 10 10 10 10 10 10 10 10 1534 ANIMAL NUMBER ------ Group: 3 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 MEAN S.D. N Group: 4 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 7.9 1.2 5.8 .7 .2 .0 15 73 9 2 0 8.0 1.1 5.9 .7 .2 .0 14 74 9 2 0 6.7 1.1 4.8 .7 .1 .0 16 71 11 2 0 8.0 1.0 6.6 .3 .1 .0 12 82 4 2 0 10.9 1.7 7.9 1.1 .2 .0 16 72 10 2 0 9.6 1.7 6.5 1.2 .1 .1 18 68 12 1 1 5.2 .7 3.6 .7 .1 .0 14 70 14 2 0 7.0 1.1 5.2 .6 .1 .0 16 74 9 1 0 8.7 1.2 6.2 1.1 .2 .0 14 70 13 2 0 6.4 .8 4.7 .5 .3 .0 13 74 8 5 0 7.8 1.2 5.7 .8 .2 .0 15 73 10 2 0 1.64 .33 1.20 .29 .07 .03 1.8 3.8 2.8 1.1 .3 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 8.4 1.7 5.7 .8 .2 .0 20 68 10 2 0 5.1 .9 3.7 .4 .1 .0 18 72 7 2 0 6.6 .7 5.0 .8 .1 .0 11 75 12 2 0 6.4 1.1 4.7 .4 .1 .0 17 74 7 2 0 6.7 1.3 4.4 .8 .2 .0 20 65 12 3 0 8.9 1.7 6.0 1.0 .2 .0 19 68 11 2 0 8.0 1.0 6.2 .5 .2 .0 13 78 6 3 0 8.7 1.7 6.0 .8 .1 .0 20 69 10 2 0 12.6 .9 10.8 .6 .2 .0 7 86 5 1 0 6.5 1.2 4.6 .6 .1 .0 18 71 9 2 0 7.8 1.2 5.7 .7 .2 .0 16 73 9 2 0 2.08 .37 1.97 .20 .05 .00 4.5 6.1 2.5 .6 .0 10 10 10 10 10 10 10 10 10 10 10 1535 ANIMAL NUMBER ------ Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 10.2 1.3 7.5 1.2 .2 .0 13 74 11 2 0 11.5 1.8 8.7 .8 .2 .0 16 76 7 2 0 18.9 1.5 16.0 1.2 .1 .1 8 84 7 1 0 7.8 1.2 4.8 1.5 .3 .0 15 62 19 3 0 7.6 1.5 5.8 .3 .1 .0 19 76 4 1 0 13.1 2.1 9.4 1.2 .3 .0 16 72 10 2 0 8.5 1.4 6.3 .6 .1 .0 17 75 7 1 0 6.0 1.6 3.8 .5 .1 .0 27 62 8 2 0 9.7 2.2 6.7 .6 .2 .0 22 70 6 2 0 11.5 1.2 9.4 .7 .1 .0 11 82 6 1 0 10.5 1.6 7.8 .9 .2 .0 16 73 8 2 0 3.65 .35 3.43 .39 .08 .03 5.4 7.3 4.2 .7 .0 10 10 10 10 10 10 10 10 10 10 10 1536 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 - - - - - C92509 - - - - - C92511 - - - - - C92521 - - - - - C92527 - - - - - C92528 - - - - - C92532 - - - - - C92535 - - - - - C92537 - - - - - C92562 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 - - - - - C92593 - - - - - C92598 - - - - - C92600 - - - - - C92612 - - - - - C92616 - - - - - C92621 - - - - - C92624 - - - - - C92627 - - - - - C92629 - - - - - Covance 6329-183 3M T-6295 1537 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 - - - - - C92640 - - - - - C92644 - - - - - C92645 - - - - - C92651 - - - - - C92661 - - - - - C92662 - - - - - C92676 - - - - - C92682 - - - - - C92684 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 - - - - - C92713 - - - - - C92714 - - - - - C92716 - - - - - C92719 - - - - - C92722 - - - - - C92726 - - - - - C92728 - - - - - C92741 - - - - - C92748 - - - - - Covance 6329-183 3M T-6295 1538 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 - - - - - C92765 - - - - - C92767 - - - - - C92774 - - - - - C92777 - - - - - C92784 - - - - - C92789 - - - - - C92799 - - - - - C92812 - - - - - C92820 - - - - - Covance 6329-183 3M T-6295 1539 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 8.49 8.00 8.90 8.61 7.73 7.65 8.13 7.78 7.55 7.17 15.9 15.5 16.8 16.1 14.9 14.7 15.7 15.0 14.4 13.9 47.9 45.8 50.3 46.8 43.1 42.9 46.0 43.9 43.5 42.2 56.4 57.3 56.5 54.4 55.7 56.0 56.6 56.4 57.7 58.8 18.8 19.4 18.9 18.7 19.2 19.2 19.4 19.2 19.1 19.4 33.2 33.9 33.5 34.4 34.5 34.3 34.2 34.1 33.1 33.0 1041 1022 1194 1054 1078 1094 1133 1027 1015 948 MEAN S.D. N 8.00 .535 10 15.3 .87 10 45.2 2.58 10 56.6 1.18 10 19.1 .25 10 33.8 .57 10 1061 68.5 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 8.37 8.65 8.46 7.60 7.90 7.90 7.89 8.24 8.40 8.75 15.0 16.2 15.8 15.0 14.5 16.0 14.9 15.9 15.9 15.8 45.8 49.6 46.6 45.3 43.9 45.2 43.3 46.5 47.6 45.8 54.7 57.4 55.1 59.6 55.6 57.1 54.9 56.4 56.6 52.4 17.9 18.7 18.7 19.8 18.3 20.2 18.9 19.3 18.9 18.0 32.7 32.6 33.9 33.2 32.9 35.4 34.4 34.2 33.3 34.4 1153 1091 1234 1136 1126 1092 1206 1009 1207 1179 MEAN S.D. N 8.22 .377 10 15.5 .59 10 46.0 1.80 10 56.0 1.93 10 18.9 .74 10 33.7 .91 10 1143 67.8 10 Covance 6329-183 3M T-6295 1540 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 7.82 7.80 8.31 7.95 8.06 8.38 8.34 7.87 7.76 8.32 15.3 14.5 15.2 15.5 14.8 15.1 16.5 14.5 15.4 15.3 46.1 42.2 44.5 45.8 45.2 44.8 49.1 44.2 45.8 44.5 58.9 54.1 53.5 57.6 56.1 53.5 58.8 56.2 59.1 53.5 19.6 18.6 18.2 19.4 18.3 18.0 19.8 18.4 19.8 18.3 33.3 34.4 34.1 33.8 32.7 33.7 33.6 32.8 33.6 34.3 993 989 1126 1118 1070 1201 1056 1192 902 1058 MEAN S.D. N 8.06 .253 10 15.2 .58 10 45.2 1.76 10 56.1 2.37 10 18.8 .72 10 33.6 .57 10 1070 93.4 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 8.40 8.55 7.84 7.30 8.09 7.49 7.81 7.51 8.15 8.56 16.0 16.1 15.0 14.5 15.2 14.6 14.6 14.3 15.8 15.9 46.8 47.0 44.0 42.9 46.5 41.7 44.1 42.5 47.4 46.9 55.7 55.0 56.1 58.8 57.5 55.7 56.5 56.6 58.1 54.7 19.0 18.8 19.2 19.8 18.8 19.5 18.6 19.0 19.3 18.5 34.2 34.1 34.1 33.7 32.7 34.9 33.0 33.6 33.2 33.9 1174 1120 1055 1088 1083 1039 1181 1025 972 1078 MEAN S.D. N 7.97 .453 10 15.2 .70 10 45.0 2.17 10 56.5 1.32 10 19.0 .41 10 33.7 .65 10 1082 64.7 10 Covance 6329-183 3M T-6295 1541 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 7.78 8.22 7.59 7.90 8.08 8.10 7.89 7.44 8.04 7.70 14.7 15.3 14.3 15.5 15.1 16.1 15.7 13.8 15.1 15.3 41.0 46.4 43.0 44.2 45.6 46.3 44.4 41.9 43.8 45.4 52.7 56.4 56.7 56.0 56.5 57.1 56.3 56.3 54.5 59.0 18.9 18.6 18.8 19.6 18.7 19.9 19.9 18.5 18.8 19.9 35.9 33.1 33.2 35.0 33.1 34.8 35.3 32.9 34.4 33.7 1033 1128 1188 1150 1024 1226 1180 1339 1250 921 MEAN S.D. N 7.87 .247 10 15.1 .67 10 44.2 1.82 10 56.2 1.64 10 19.2 .59 10 34.1 1.08 10 1144 122.9 10 Covance 6329-183 3M T-6295 1542 ANIMAL NUMBER ------ Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 MEAN S.D. N Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 4.5 .7 3.0 .6 .2 .0 15 67 14 4 0 6.4 2.1 3.4 .7 .1 .0 33 54 11 2 0 6.5 1.5 4.4 .4 .1 .0 24 68 7 1 0 2.3 .8 1.3 .1 .0 .0 34 58 5 2 0 3.5 .9 2.1 .4 .1 .0 25 60 12 2 0 6.6 1.3 4.6 .5 .2 .0 20 70 7 3 0 4.4 .7 3.3 .3 .1 .0 17 75 6 3 0 3.7 .3 3.1 .3 .0 .0 9 83 7 1 0 6.4 .7 5.1 .4 .1 .0 11 80 7 2 0 4.6 .6 3.5 .4 .1 .0 13 77 8 2 0 4.9 1.0 3.4 .4 .1 .0 20 69 8 2 0 1.51 .53 1.14 .17 .07 .00 8.8 9.7 2.9 .9 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 4.4 .8 3.0 .5 .1 .0 18 68 12 2 0 9.6 1.7 7.0 .7 .1 .0 18 73 7 1 0 9.0 1.4 7.0 .5 .1 .0 15 78 6 1 0 10.2 1.8 7.2 1.0 .1 .0 18 71 9 1 0 3.1 .9 1.6 .5 .1 .0 28 53 16 3 0 4.5 .5 3.6 .2 .1 .0 12 81 5 2 0 4.0 .7 3.0 .2 .0 .0 17 76 6 1 0 6.1 1.0 4.5 .6 .1 .0 16 73 9 2 0 5.0 .7 3.5 .7 .1 .0 15 69 13 3 0 8.6 .7 7.5 .3 .1 .0 8 87 3 1 0 6.4 1.0 4.8 .5 .1 .0 16 73 9 2 0 2.64 .45 2.18 .25 .03 .00 5.1 9.1 4.0 .8 .0 10 10 10 10 10 10 10 10 10 10 10 1543 ANIMAL NUMBER ------ Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 MEAN S.D. N Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 4.1 .9 2.9 .3 .1 .0 21 70 7 2 0 3.8 .4 3.1 .3 .0 .0 11 80 7 1 0 3.8 .7 2.7 .3 .1 .0 18 71 8 2 0 6.4 .7 5.3 .3 .1 .0 10 83 5 2 0 3.8 .6 2.9 .3 .1 .0 15 75 8 3 0 3.0 .5 2.2 .2 .1 .0 18 74 5 3 0 4.5 .8 3.4 .3 .1 .0 17 75 6 1 0 7.7 .9 6.3 .4 .1 .0 12 81 5 1 0 4.8 .6 3.8 .3 .1 .0 12 79 7 3 0 7.4 .5 6.2 .5 .1 .0 7 85 7 1 0 4.9 .7 3.9 .3 .1 .0 14 77 6 2 0 1.65 .17 1.50 .08 .03 .00 4.4 5.1 1.2 .9 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 6.0 .6 5.2 .2 .1 .0 10 85 3 1 0 3.2 .6 2.1 .3 .0 .0 20 68 11 1 1 5.8 .4 5.1 .2 .1 .0 7 88 4 1 0 6.5 .8 5.1 .5 .2 .0 12 78 7 3 0 5.1 1.2 3.3 .4 .1 .0 24 65 8 2 0 4.4 .5 3.5 .3 .1 .0 10 80 7 2 0 8.3 .5 6.2 1.3 .1 .1 6 75 16 1 1 9.6 2.2 6.8 .4 .1 .0 23 71 4 1 0 5.8 1.3 4.1 .3 .1 .0 23 69 6 2 0 3.8 .7 2.6 .3 .1 .0 19 69 9 3 0 5.8 .9 4.4 .4 .1 .0 15 75 8 2 0 1.96 .55 1.54 .32 .05 .03 7.1 7.7 3.9 .8 .4 10 10 10 10 10 10 10 10 10 10 10 1544 ANIMAL NUMBER ------ Group: 5 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 4.8 .8 3.7 .1 .1 .0 17 78 3 3 0 7.2 1.0 5.5 .6 .1 .0 15 76 8 1 0 5.4 .7 4.0 .6 .1 .0 13 73 12 2 1 3.1 .6 2.2 .2 .1 .0 18 72 7 3 0 5.7 1.1 4.1 .4 .1 .0 19 71 8 2 0 3.2 .7 2.0 .3 .2 .0 23 62 10 5 0 3.2 .5 2.5 .2 .1 .0 15 76 6 3 0 7.6 2.7 4.4 .4 .1 .0 35 58 6 1 0 3.4 .6 2.7 .2 .0 .0 16 78 5 1 0 4.0 .8 3.0 .3 .0 .0 19 74 7 1 0 4.8 1.0 3.4 .3 .1 .0 19 72 7 2 0 1.68 .64 1.12 .17 .06 .00 6.3 6.7 2.5 1.3 .3 10 10 10 10 10 10 10 10 10 10 10 1545 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 - - - - - C92865 - - - - - C92871 - - - - - C92880 - - - - - C92887 - - - - - C92898 - - - - - C92903 - - - - - C92905 - - - - - C92906 - - - - - C92927 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 - - - - - C92940 - - - - - C92944 - - - - - C92948 - - - - - C92956 - - - - - C92962 - - - - - C92967 - - - - - C92978 - - - - - C92986 - - - - - C92987 - - - - - Covance 6329-183 3M T-6295 1546 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 - - - - - C92993 - - - - - C93000 - - - - - C93018 - - - - - C93019 - - - - - C93023 - - - - - C93024 - - - - - C93026 - - - - - C93030 - - - - - C93035 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 - - - - - C93064 - - - - - C93071 - - - - - C93076 - - - - - C93078 - - - - - C93085 - - - - - C93089 - - - - - C93090 - - - - - C93093 - - - - - C93109 - - - - - Covance 6329-183 3M T-6295 1547 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 - - - - - C93113 - - - - - C93120 - - - - - C93127 - - - - - C93135 - - - - - C93143 - - - - - C93155 - - - - - C93162 - - - - - C93169 - - - - - C93178 - - - - - Covance 6329-183 3M T-6295 1548 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 8.48 8.86 8.77 8.82 8.32 8.18 8.90 8.51 9.20 9.81 15.6 15.8 16.3 15.2 15.0 14.8 15.6 15.8 16.2 16.6 46.3 46.0 48.4 45.3 43.7 42.8 45.8 46.2 47.8 49.1 54.5 52.0 55.2 51.4 52.5 52.3 51.5 54.3 52.0 50.1 18.3 17.8 18.6 17.3 18.1 18.0 17.5 18.6 17.6 16.9 33.6 34.3 33.6 33.6 34.4 34.4 34.0 34.3 33.8 33.8 1112 1185 965 1414 1325 1130 1040 1318 1464 1394 MEAN S.D. N 8.78 .471 10 15.7 .58 10 46.1 1.96 10 52.6 1.60 10 17.9 .56 10 34.0 .34 10 1235 171.4 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 9.24 8.48 7.82 9.59 8.58 8.83 8.11 9.84 9.11 8.83 16.2 15.2 13.7 15.7 15.3 15.2 15.0 16.8 15.8 16.1 48.0 44.8 41.3 47.2 45.7 44.9 43.9 49.4 46.8 47.6 52.0 52.8 52.9 49.2 53.3 50.8 54.1 50.3 51.3 53.9 17.6 18.0 17.6 16.4 17.8 17.2 18.5 17.1 17.4 18.2 33.8 34.0 33.2 33.3 33.4 33.7 34.1 34.0 33.8 33.7 1197 1249 1262 1294 1483 1291 1003 1139 1360 1424 MEAN S.D. N 8.84 .629 10 15.5 .84 10 46.0 2.34 10 52.1 1.63 10 17.6 .60 10 33.7 .31 10 1270 138.4 10 Covance 6329-183 3M T-6295 1549 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 8.78 8.46 8.57 8.56 9.53 8.87 8.96 8.69 8.82 8.92 15.9 14.9 14.8 14.8 16.2 15.7 16.0 15.7 15.7 15.0 46.2 44.2 43.4 43.9 48.5 46.7 47.2 45.9 45.7 44.5 52.6 52.2 50.7 51.3 50.9 52.7 52.6 52.8 51.8 49.9 18.1 17.6 17.3 17.2 17.0 17.8 17.8 18.1 17.8 16.8 34.5 33.8 34.1 33.6 33.4 33.7 33.8 34.3 34.4 33.6 1325 1313 1003 1112 1219 1243 1324 1384 1097 1354 MEAN S.D. N 8.82 .301 10 15.5 .54 10 45.6 1.62 10 51.8 1.01 10 17.5 .45 10 33.9 .38 10 1237 127.7 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 8.70 9.46 10.00 9.10 8.80 8.63 9.08 9.19 8.53 8.95 15.8 16.6 16.4 16.1 15.1 15.4 16.1 15.4 14.7 16.1 46.1 48.4 49.3 47.5 44.4 45.6 47.2 46.3 43.4 48.1 53.0 51.2 49.2 52.2 50.5 52.8 52.0 50.3 50.9 53.7 18.1 17.5 16.3 17.7 17.1 17.9 17.7 16.7 17.2 17.9 34.2 34.2 33.2 33.9 33.9 33.8 34.1 33.3 33.8 33.4 1227 1289 1300 1176 1397 1072 1303 1074 1062 1269 MEAN S.D. N 9.04 .439 10 15.8 .60 10 46.6 1.84 10 51.6 1.40 10 17.4 .58 10 33.8 .36 10 1217 116.4 10 Covance 6329-183 3M T-6295 1550 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 8.64 8.64 8.71 9.27 8.59 8.28 8.75 8.46 8.70 8.66 14.8 16.4 15.2 15.2 14.6 14.9 14.6 14.8 14.6 16.0 44.6 47.6 44.5 44.7 44.0 44.6 43.2 44.5 43.9 45.9 51.6 55.1 51.1 48.2 51.3 53.8 49.4 52.6 50.4 53.0 17.2 19.0 17.4 16.4 17.0 18.0 16.7 17.5 16.8 18.5 33.3 34.5 34.1 34.0 33.2 33.4 33.8 33.3 33.3 34.8 1362 1349 1367 1342 1068 1021 1394 1473 1087 1410 MEAN S.D. N 8.67 .252 10 15.1 .62 10 44.7 1.22 10 51.6 2.06 10 17.4 .83 10 33.8 .57 10 1287 162.9 10 Covance 6329-183 3M T-6295 1551 ANIMAL NUMBER ------ Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 MEAN S.D. N Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 8.0 1.2 5.6 1.0 .1 .1 16 70 13 1 1 8.9 1.2 6.0 1.3 .3 .0 14 68 15 3 0 7.4 1.7 4.6 1.0 .1 .1 23 62 14 1 1 10.6 1.6 7.6 1.2 .2 .1 15 72 11 2 1 7.6 .8 5.5 1.1 .2 .1 10 72 14 2 1 5.8 1.2 3.9 .5 .1 .0 21 68 9 2 0 6.4 1.1 4.3 .9 .1 .0 17 67 14 1 1 6.7 1.2 4.3 1.1 .1 .1 17 64 16 2 1 7.6 1.7 4.7 1.0 .3 .0 22 61 13 4 0 6.2 1.3 4.0 .7 .2 .0 21 64 11 3 1 7.5 1.3 5.0 1.0 .2 .0 18 67 13 2 1 1.43 .29 1.14 .23 .08 .05 4.1 3.9 2.1 1.0 .5 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 8.5 1.4 6.2 .7 .2 .0 16 72 8 3 0 7.6 1.6 4.6 1.1 .2 .1 22 61 15 2 1 6.5 1.4 4.2 .7 .2 .0 22 64 10 3 1 7.5 1.9 4.6 .8 .2 .0 25 61 11 3 1 8.7 1.4 6.4 .7 .2 .0 16 73 8 3 0 8.4 1.1 6.1 1.0 .2 .0 13 73 12 2 1 11.5 1.7 8.4 1.1 .2 .1 15 73 10 1 1 6.7 2.0 3.8 .7 .2 .0 29 57 10 3 0 7.8 1.3 5.2 1.1 .2 .0 16 67 14 3 1 9.1 1.1 6.7 1.1 .1 .1 12 73 12 2 1 8.2 1.5 5.6 .9 .2 .0 19 67 11 2 1 1.42 .31 1.40 .19 .03 .05 5.6 6.2 2.3 .7 .5 10 10 10 10 10 10 10 10 10 10 10 1552 ANIMAL NUMBER ------ Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 MEAN S.D. N Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 7.8 1.8 4.9 .9 .2 .0 22 64 11 2 0 5.8 1.2 3.8 .7 .1 .0 20 66 11 1 1 8.3 1.1 6.2 .8 .2 .0 13 75 9 2 0 9.4 2.4 5.5 1.2 .2 .0 26 58 13 3 0 7.6 1.1 5.5 .7 .1 .1 15 73 9 2 1 9.4 1.3 6.9 1.0 .2 .0 14 73 10 2 0 7.4 2.4 3.7 1.2 .2 .0 32 50 16 2 0 10.2 1.5 7.4 1.1 .2 .0 14 72 11 2 0 10.6 2.2 6.8 1.2 .3 .0 21 64 12 3 0 10.1 1.7 6.5 1.5 .3 .1 17 65 14 3 1 8.7 1.7 5.7 1.0 .2 .0 19 66 12 2 0 1.53 .52 1.28 .26 .07 .04 6.1 7.8 2.2 .6 .5 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 7.6 1.2 5.6 .6 .2 .0 15 74 8 2 0 8.8 1.7 5.5 1.3 .2 .1 19 62 15 2 1 10.1 1.7 6.3 1.8 .2 .0 17 62 18 2 0 6.3 1.5 3.7 .9 .2 .0 24 59 14 2 0 9.1 2.0 5.7 1.2 .1 .0 23 62 13 2 0 9.6 2.0 6.3 1.1 .2 .1 20 66 12 2 1 12.5 1.1 9.9 1.2 .2 .1 9 79 10 1 1 8.8 1.9 5.3 1.3 .2 .1 21 61 15 2 1 6.5 1.4 4.1 .9 .1 .0 22 63 13 1 1 7.1 1.6 4.2 1.2 .1 .1 22 58 17 2 1 8.6 1.6 5.7 1.2 .2 .0 19 65 14 2 1 1.88 .31 1.74 .32 .05 .05 4.5 6.7 3.0 .4 .5 10 10 10 10 10 10 10 10 10 10 10 1553 ANIMAL NUMBER ------ Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 6.4 1.0 4.4 .9 .1 .0 15 69 14 1 0 12.2 2.1 8.4 1.5 .1 .1 17 69 12 1 1 7.0 1.6 4.3 .9 .2 .0 22 61 14 2 1 7.0 1.2 5.0 .6 .2 .0 18 71 8 3 1 8.5 1.8 5.2 1.3 .1 .0 22 61 15 2 0 9.9 2.2 6.3 1.2 .1 .0 22 64 12 1 0 10.3 1.8 7.3 1.0 .1 .1 18 71 10 1 1 6.8 1.1 4.7 .8 .2 .0 16 70 12 2 0 9.6 1.4 6.9 1.1 .2 .0 14 72 12 2 0 9.0 1.3 6.9 .7 .1 .0 14 76 7 1 0 8.7 1.6 5.9 1.0 .1 .0 18 68 12 2 0 1.88 .42 1.41 .28 .05 .04 3.2 4.9 2.6 .7 .5 10 10 10 10 10 10 10 10 10 10 10 1554 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 - - - - - C92510 - - - - - C92512 - - - - - C92525 - - - - - C92537 - - - - - C92543 - - - - - C92545 - - - - - C92563 - - - - - C92567 - - - - - C92570 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 - - - - - C92583 - - - - - C92585 - - - - - C92586 - - - - - C92588 - - - - - C92590 - - - - - C92601 - - - - - C92602 - - - - - C92617 - - - - - C92630 - - - - - Covance 6329-183 3M T-6295 1555 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 - - - - - C92644 - - - - - C92647 - - - - - C92658 - - - - - C92660 - - - - - C92667 - - - - - C92673 - - - - - C92679 - - - - - C92686 - - - - - C92687 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 - - - - - C92698 - - - - - C92708 - - - - - C92709 - - - - - C92710 - - - - - C92725 - - - - - C92743 - - - - - C92745 - - - - - C92749 - - - - - C92750 - - - - - Covance 6329-183 3M T-6295 1556 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 - - - - - C92761 - - - - - C92764 - - - - - C92773 - - - - - C92783 - - - - - C92788 - - - - - C92791 - - - - - C92803 - - - - - C92804 - - - - - C92820 - - - - - Covance 6329-183 3M T-6295 1557 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 7.70 7.02 8.77 6.91 7.04 7.40 7.66 7.28 7.77 8.04 15.0 13.7 16.3 13.3 13.6 14.4 14.6 13.8 15.3 15.5 45.1 40.6 49.8 39.9 41.4 41.2 44.2 41.2 45.8 45.9 58.6 57.8 56.8 57.8 58.8 55.6 57.7 56.6 58.9 57.1 19.5 19.5 18.6 19.2 19.3 19.5 19.1 18.9 19.7 19.2 33.3 33.8 32.8 33.2 32.8 35.0 33.1 33.4 33.4 33.6 1168 1150 1102 841 1043 1152 1057 828 1102 1071 MEAN S.D. N 7.56 .563 10 14.6 .97 10 43.5 3.16 10 57.6 1.06 10 19.2 .33 10 33.4 .63 10 1051 121.7 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 6.36 8.39 8.07 7.82 7.35 8.16 8.28 8.47 8.30 8.08 14.0 15.3 15.0 14.7 14.1 15.0 15.7 15.0 15.1 15.2 40.9 46.1 45.1 43.9 42.4 45.7 47.4 45.2 45.5 46.0 64.3 54.9 55.9 56.2 57.7 56.0 57.2 53.4 54.9 57.0 22.0 18.2 18.6 18.8 19.2 18.4 18.9 17.7 18.2 18.9 34.2 33.2 33.3 33.4 33.3 32.9 33.1 33.2 33.2 33.1 797 1158 1116 1085 1001 1205 930 1025 1201 1049 MEAN S.D. N 7.93 .639 10 14.9 .52 10 44.8 1.92 10 56.8 2.94 10 18.9 1.18 10 33.3 .35 10 1057 127.0 10 Covance 6329-183 3M T-6295 1558 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 7.70 8.19 8.15 7.79 8.59 7.85 7.49 8.22 7.96 8.03 14.5 15.2 15.7 14.4 16.2 15.7 14.4 15.4 14.4 15.0 43.6 45.5 46.8 43.3 48.9 46.4 43.2 45.7 42.9 45.9 56.6 55.6 57.4 55.6 56.9 59.1 57.7 55.6 53.9 57.1 18.8 18.5 19.2 18.5 18.8 20.0 19.3 18.8 18.1 18.7 33.2 33.3 33.5 33.3 33.1 33.8 33.4 33.7 33.6 32.7 934 1154 1080 1123 1119 979 1070 1099 1163 1023 MEAN S.D. N 8.00 .312 10 15.1 .66 10 45.2 1.94 10 56.5 1.44 10 18.9 .53 10 33.4 .32 10 1074 75.0 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 8.61 8.04 8.38 7.74 8.40 7.84 8.61 7.69 7.86 8.00 15.7 15.9 16.1 14.3 15.6 14.4 15.7 14.9 15.4 15.4 47.2 46.4 47.9 43.4 46.9 43.9 48.1 44.8 45.6 46.5 54.9 57.7 57.1 56.1 55.8 56.0 55.8 58.3 57.9 58.2 18.2 19.7 19.1 18.5 18.6 18.4 18.3 19.4 19.6 19.3 33.2 34.2 33.5 33.0 33.3 32.9 32.7 33.3 33.8 33.1 1098 1065 1358 1056 1240 1092 1160 925 1013 944 MEAN S.D. N 8.12 .353 10 15.3 .61 10 46.1 1.61 10 56.8 1.20 10 18.9 .57 10 33.3 .44 10 1095 131.4 10 Covance 6329-183 3M T-6295 1559 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 7.83 7.52 7.46 8.05 8.24 7.96 8.04 6.97 7.31 7.91 14.8 13.9 14.6 14.3 15.1 14.6 14.9 13.3 14.6 14.7 44.2 41.6 43.2 43.0 46.1 44.0 43.3 39.7 43.7 44.1 56.5 55.4 57.9 53.4 55.9 55.3 53.8 56.9 59.8 55.7 19.0 18.5 19.6 17.7 18.4 18.4 18.5 19.1 20.0 18.6 33.6 33.3 33.9 33.2 32.9 33.3 34.3 33.6 33.5 33.3 1040 1065 867 907 1229 1075 1081 848 1278 1233 MEAN S.D. N 7.73 .398 10 14.5 .53 10 43.3 1.70 10 56.1 1.87 10 18.8 .66 10 33.5 .39 10 1062 153.7 10 Covance 6329-183 3M T-6295 1560 ANIMAL NUMBER ------ Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 MEAN S.D. N Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 5.2 1.1 3.4 .5 .1 .0 22 66 10 2 0 5.5 2.0 3.0 .5 .1 .0 36 53 9 2 0 6.0 .4 4.8 .7 .1 .0 7 79 11 2 0 5.1 .9 3.8 .3 .1 .0 17 74 7 2 1 6.9 .9 5.1 .5 .4 .0 12 74 7 6 0 5.0 .4 3.8 .6 .1 .0 9 75 13 2 0 5.0 .6 3.8 .5 .2 .0 12 75 10 3 0 5.1 .3 4.4 .3 .1 .0 6 87 6 2 0 6.8 1.5 4.0 1.2 .1 .1 22 59 17 1 1 4.8 .7 3.7 .4 .1 .0 14 76 8 2 0 5.5 .9 4.0 .6 .1 .0 16 72 10 2 0 .77 .54 .63 .26 .10 .03 9.1 9.9 3.3 1.3 .4 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 19.3 4.4 13.2 1.3 .2 .1 23 69 7 1 0 3.3 .4 2.4 .4 .1 .0 11 74 13 2 0 6.2 .4 5.4 .4 .1 .0 6 87 6 1 0 2.8 .6 2.1 .1 .1 .0 21 73 4 2 0 7.6 1.2 5.8 .5 .2 .0 15 76 7 2 0 3.2 .4 2.6 .2 .1 .0 11 82 5 2 0 5.8 .8 4.5 .5 .1 .0 13 77 8 2 0 5.0 .5 4.1 .4 .1 .0 9 81 8 1 0 5.1 .8 3.6 .6 .1 .0 15 70 12 3 1 8.6 1.5 6.6 .3 .2 .0 18 77 3 2 0 6.7 1.1 5.0 .5 .1 .0 14 77 7 2 0 4.82 1.22 3.24 .33 .05 .03 5.3 5.6 3.2 .6 .3 10 10 10 10 10 10 10 10 10 10 10 1561 ANIMAL NUMBER ------ Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 MEAN S.D. N Group: 4 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 6.9 1.0 5.3 .4 .1 .0 15 77 6 1 0 5.7 1.1 3.7 .8 .1 .0 19 65 14 1 0 4.7 .5 3.9 .2 .1 .0 10 84 4 2 0 5.6 .7 4.5 .3 .1 .0 12 80 6 1 0 4.8 .7 3.6 .3 .2 .0 15 75 6 4 0 5.3 .7 4.0 .5 .1 .0 12 75 10 3 0 3.2 .5 2.5 .2 .1 .0 15 78 5 2 0 4.0 .8 2.8 .3 .1 .0 20 70 8 2 0 4.6 .8 3.3 .4 .1 .0 16 72 9 2 0 3.1 .5 2.0 .4 .1 .0 17 66 13 4 0 4.8 .7 3.6 .4 .1 .0 15 74 8 2 0 1.17 .21 .97 .18 .03 .00 3.1 6.0 3.4 1.1 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 7.7 .9 5.8 .7 .2 .0 12 76 9 2 0 5.4 .7 4.1 .4 .1 .0 13 77 8 1 0 5.2 .7 4.1 .3 .2 .0 13 78 6 3 0 5.2 .7 3.8 .6 .1 .0 13 73 12 1 1 5.5 .5 4.6 .3 .1 .0 10 84 5 1 0 10.5 1.3 8.6 .4 .2 .0 12 82 4 2 0 4.6 .6 3.7 .3 .1 .0 13 79 6 2 0 3.9 1.0 2.6 .2 .1 .0 26 66 6 2 0 7.7 .7 6.3 .6 .1 .0 8 82 7 2 1 5.6 .9 3.6 1.0 .1 .0 16 65 17 2 0 6.1 .8 4.7 .5 .1 .0 14 76 8 2 0 1.95 .23 1.74 .24 .05 .00 4.8 6.5 3.9 .6 .4 10 10 10 10 10 10 10 10 10 10 10 1562 ANIMAL NUMBER ------ Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 3.4 .5 2.6 .3 .1 .0 15 74 8 3 0 6.6 1.2 4.5 .6 .2 .0 19 69 9 3 1 5.0 .6 4.0 .3 .1 .0 13 80 6 1 0 5.6 .5 4.7 .2 .1 .0 9 84 4 2 0 6.2 .8 5.2 .1 .1 .0 13 84 1 2 0 8.2 1.1 6.2 .6 .2 .0 13 76 8 3 1 8.0 1.1 6.5 .3 .1 .0 13 82 4 1 0 6.3 2.3 3.3 .6 .1 .0 37 52 9 2 0 3.5 .8 2.2 .4 .1 .0 22 62 12 4 1 5.8 .5 4.8 .4 .1 .0 8 83 8 1 0 5.9 .9 4.4 .4 .1 .0 16 75 7 2 0 1.61 .55 1.41 .18 .04 .00 8.4 10.7 3.2 1.0 .5 10 10 10 10 10 10 10 10 10 10 10 1563 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 - - - - - C92878 - - - - - C92888 - - - - - C92906 - - - - - C92916 - - - - - C92917 - - - - - C92921 - - - - - C92922 - - - - - C92923 - - - - - C92928 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 - - - - - C92935 - - - - - C92938 - - - - - C92951 - - - - - C92954 - - - - - C92956 - - - - - C92960 - - - - - C92974 - - - - - C92979 - - - - - C92989 - - - - - Covance 6329-183 3M T-6295 1564 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 - - - - - C93004 - - - - - C93011 - - - - - C93020 - - - - - C93027 - - - - - C93030 - - - - - C93034 - - - - - C93046 - - - - - C93047 - - - - - C93049 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 - - - - - C93055 - - - - - C93072 - - - - - C93080 - - - - - C93081 - - - - - C93090 - - - - - C93094 - - - - - C93095 - - - - - C93103 - - - - - C93104 - - - - - Covance 6329-183 3M T-6295 1565 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 - - - - - C93131 - - - - - C93133 - - - - - C93138 - - - - - C93149 - - - - - C93153 - - - - - C93157 - - - - - C93160 - - - - - C93162 - - - - - C93179 - - - - - Covance 6329-183 3M T-6295 1566 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 8.22 8.32 8.64 8.24 7.98 7.77 8.55 8.39 8.59 8.58 15.5 15.3 16.0 15.6 15.6 13.7 15.9 15.8 16.2 15.2 43.4 43.8 44.0 44.1 43.5 38.5 45.4 44.3 45.9 42.5 52.8 52.6 50.9 53.5 54.5 49.6 53.1 52.8 53.5 49.5 18.9 18.4 18.5 19.0 19.5 17.6 18.6 18.9 18.9 17.7 35.8 35.0 36.3 35.5 35.8 35.6 35.1 35.8 35.4 35.7 1161 971 1103 919 1206 1194 1234 890 1056 1388 MEAN S.D. N 8.33 .286 10 15.5 .70 10 43.5 2.02 10 52.3 1.70 10 18.6 .59 10 35.6 .38 10 1112 155.9 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 8.44 8.55 8.73 8.20 8.70 8.34 8.04 8.27 8.03 8.04 15.4 14.9 15.4 15.3 15.3 15.0 15.1 15.6 15.1 15.2 43.6 43.7 45.0 43.5 43.7 42.5 42.6 43.7 42.4 43.5 51.7 51.1 51.5 53.1 50.2 50.9 53.0 52.8 52.7 54.2 18.3 17.5 17.7 18.6 17.6 18.0 18.8 18.8 18.8 19.0 35.3 34.2 34.3 35.1 35.0 35.4 35.4 35.6 35.6 35.0 992 1414 924 1049 887 1038 1124 955 1292 915 MEAN S.D. N 8.33 .266 10 15.2 .21 10 43.4 .77 10 52.1 1.23 10 18.3 .57 10 35.1 .49 10 1059 172.9 10 Covance 6329-183 3M T-6295 1567 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 8.23 8.01 8.26 8.32 8.25 7.89 8.26 8.16 7.40 8.35 14.2 15.8 15.2 14.8 14.9 14.1 15.5 15.6 14.0 15.0 41.4 44.7 43.0 42.3 42.2 40.1 43.8 44.2 40.0 43.5 50.3 55.8 52.0 50.8 51.1 50.9 53.0 54.2 54.0 52.1 17.3 19.8 18.4 17.8 18.0 17.9 18.8 19.1 18.9 18.0 34.4 35.5 35.4 35.1 35.2 35.1 35.4 35.3 34.9 34.6 913 830 944 938 1127 952 1053 838 963 752 MEAN S.D. N 8.11 .288 10 14.9 .64 10 42.5 1.63 10 52.4 1.79 10 18.4 .75 10 35.1 .36 10 931 108.6 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 8.47 7.88 8.59 8.36 9.14 7.29 8.88 8.65 8.81 8.20 15.2 14.8 14.7 14.7 15.7 14.7 15.2 15.6 15.9 15.1 43.3 42.2 42.6 42.5 45.5 42.2 45.5 44.0 46.4 42.8 51.1 53.6 49.5 50.8 49.7 57.8 51.3 50.9 52.6 52.2 17.9 18.8 17.2 17.6 17.2 20.2 17.1 18.0 18.0 18.5 35.0 35.1 34.7 34.6 34.6 35.0 33.4 35.3 34.3 35.4 832 1037 1199 1062 1001 900 1124 910 964 1078 MEAN S.D. N 8.43 .536 10 15.2 .45 10 43.7 1.56 10 52.0 2.41 10 18.0 .94 10 34.7 .58 10 1011 111.9 10 Covance 6329-183 3M T-6295 1568 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 7.98 8.70 8.74 8.14 8.46 8.28 7.85 7.81 8.60 14.3 15.6 15.9 14.3 15.2 14.3 13.9 14.8 15.5 40.3 43.1 44.0 40.6 43.3 40.5 38.8 42.4 42.6 50.5 49.6 50.3 49.9 51.2 48.9 49.5 54.2 49.5 17.9 18.0 18.1 17.6 17.9 17.3 17.7 19.0 18.0 35.3 36.2 36.0 35.3 35.0 35.4 35.7 35.0 36.3 1011 1367 1367 1378 1231 1186 1246 1114 1271 MEAN S.D. N 8.28 .360 9 14.9 .71 9 41.7 1.74 9 50.4 1.58 9 17.9 .47 9 35.6 .49 9 1241 124.3 9 Covance 6329-183 3M T-6295 1569 ANIMAL NUMBER ------ Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 MEAN S.D. N Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 6.1 1.8 3.1 1.0 .1 .0 29 52 17 1 1 6.7 3.2 2.8 .6 .1 .0 47 42 9 2 0 6.6 1.3 4.4 .8 .1 .0 19 66 12 1 1 6.3 2.2 3.0 1.1 .1 .0 34 47 17 1 0 4.6 .8 3.0 .7 .1 .0 18 65 14 3 0 9.1 2.3 5.7 1.0 .2 .0 25 62 11 2 0 8.6 3.4 3.8 1.3 .2 .0 39 43 15 2 0 7.0 3.9 2.4 .6 .1 .0 56 34 8 2 0 7.6 2.6 3.9 1.0 .1 .0 34 52 13 2 0 8.2 2.9 3.8 1.3 .2 .0 36 47 16 2 0 7.1 2.4 3.6 .9 .1 .0 34 51 13 2 0 1.33 .96 .96 .26 .05 .00 11.9 10.6 3.2 .6 .4 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 7.1 1.8 4.1 1.0 .1 .0 25 58 14 2 0 10.2 2.3 6.4 1.3 .2 .0 22 63 13 2 0 9.4 1.9 6.0 1.3 .1 .0 20 64 14 1 1 5.2 1.4 3.2 .5 .0 .0 27 62 10 1 0 6.7 1.5 4.1 1.0 .1 .0 22 61 15 2 0 8.0 1.3 5.6 .9 .2 .0 16 70 12 2 0 6.2 1.4 3.6 1.1 .1 .0 22 58 17 2 0 8.3 1.7 5.6 .9 .1 .0 20 68 11 1 0 9.6 1.9 5.7 1.7 .2 .1 20 59 18 3 1 7.8 1.8 4.9 1.0 .1 .0 22 63 13 1 0 7.8 1.7 4.9 1.1 .1 .0 22 63 14 2 0 1.59 .31 1.10 .32 .06 .03 3.0 4.0 2.5 .7 .4 10 10 10 10 10 10 10 10 10 10 10 1570 ANIMAL NUMBER ------ Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 MEAN S.D. N Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 7.6 2.0 4.6 .9 .1 .0 26 61 11 1 0 7.1 1.3 4.8 .9 .1 .0 18 67 13 1 0 16.6 6.7 7.2 2.6 .1 .1 40 43 15 1 0 7.4 1.6 5.7 .0 .1 .0 21 77 0 1 0 9.0 1.5 6.1 1.2 .1 .0 16 68 14 1 0 6.7 1.8 3.7 1.0 .2 .0 26 55 16 2 0 8.8 1.1 6.8 .7 .1 .0 12 78 8 1 0 6.3 1.6 3.8 .8 .1 .0 25 61 12 2 0 7.4 1.5 4.9 .9 .1 .0 20 66 12 1 0 9.1 1.8 6.1 1.1 .2 .0 20 67 12 2 0 8.6 2.1 5.4 1.0 .1 .0 22 64 11 1 0 2.97 1.64 1.20 .65 .04 .03 7.6 10.2 4.5 .5 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 9.2 2.3 5.4 1.3 .2 .0 25 58 14 2 0 8.5 1.6 6.0 .8 .1 .0 19 70 10 1 0 6.8 2.1 4.1 .5 .1 .0 31 60 7 2 0 6.9 1.6 4.4 .7 .1 .0 23 64 11 2 0 9.0 2.2 5.3 1.3 .2 .0 25 58 14 2 0 7.4 1.7 4.8 .7 .1 .0 23 66 9 2 0 11.7 3.9 6.2 1.5 .1 .0 33 52 13 1 0 10.5 1.2 8.4 .7 .1 .0 11 80 7 1 0 8.4 1.7 5.6 .9 .1 .0 21 67 11 1 0 7.0 1.9 4.1 .8 .1 .0 27 59 12 2 0 8.5 2.0 5.4 .9 .1 .0 24 63 11 2 0 1.63 .74 1.28 .33 .04 .00 6.2 7.9 2.6 .5 .0 10 10 10 10 10 10 10 10 10 10 10 1571 ANIMAL NUMBER ------ Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 7.9 1.5 6.3 .0 .1 .0 19 80 0 1 0 10.5 6.3 2.6 1.4 .1 .1 60 25 13 1 1 7.8 1.8 4.7 1.1 .2 .0 24 60 14 2 0 7.4 2.4 3.8 1.0 .1 .0 32 52 14 1 0 4.7 1.0 3.0 .5 .1 .0 22 64 12 2 0 6.8 1.8 4.0 .8 .1 .0 27 59 12 2 0 6.6 2.4 3.0 1.2 .1 .0 36 45 18 1 0 8.6 2.1 5.5 .8 .1 .0 25 64 10 2 0 5.0 1.3 2.9 .7 .1 .0 26 58 14 2 0 7.3 2.3 4.0 .8 .1 .0 30 56 12 2 0 1.78 1.58 1.29 .42 .03 .03 12.3 15.1 5.0 .5 .3 9 9 9 9 9 9 9 9 9 9 9 1572 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 - - - - - C92504 - - - - - C92507 - - - - - C92512 - - - - - C92517 - - - - - C92523 - - - - - C92534 - - - - - C92540 - - - - - C92562 - - - - - C92570 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 - - - - - C92587 - - - - - C92592 - - - - - C92596 - - - - - C92604 - - - - - C92612 - - - - - C92614 - - - - - C92624 - - - - - C92625 - - - - - C92629 - - - - - Covance 6329-183 3M T-6295 1573 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 - - - - - C92635 - - - - - C92643 - - - - - C92649 - - - - - C92653 - - - - - C92654 - - - - - C92667 - - - - - C92669 - - - - - C92680 - - - - - C92686 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 - - - - - C92704 - - - - - C92710 - - - - - C92711 - - - - - C92727 - - - - - C92729 - - - - - C92739 - - - - - C92741 - - - - - C92742 - - - - - C92748 - - - - - Covance 6329-183 3M T-6295 1574 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 - - - - - C92754 - - - - - C92755 - - - - - C92778 - - - - - C92790 - - - - - C92795 - - - - - C92796 - - - - - C92817 - - - - - C92820 - - - - - Covance 6329-183 3M T-6295 1575 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 8.39 7.33 7.40 7.72 7.01 7.77 7.24 7.40 6.99 6.66 16.3 14.5 14.3 14.6 13.9 14.9 14.6 13.9 13.9 13.7 44.6 40.6 40.3 41.0 39.0 41.9 40.9 38.9 39.2 38.3 53.2 55.4 54.5 53.2 55.6 54.0 56.6 52.6 56.0 57.6 19.4 19.7 19.3 18.9 19.9 19.1 20.2 18.7 19.9 20.6 36.5 35.6 35.5 35.5 35.7 35.4 35.7 35.6 35.5 35.7 1127 995 888 1055 1010 988 1146 947 992 941 MEAN S.D. N 7.39 .485 10 14.5 .76 10 40.5 1.84 10 54.9 1.64 10 19.6 .60 10 35.7 .31 10 1009 80.9 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 6.83 7.47 8.40 6.79 7.18 8.28 6.23 6.61 7.25 7.53 14.2 13.9 16.4 13.3 14.1 15.5 12.9 13.9 13.6 14.5 40.1 40.4 48.0 38.3 40.1 44.3 37.5 39.7 39.5 42.3 58.8 54.1 57.1 56.3 55.8 53.5 60.2 60.1 54.4 56.2 20.8 18.5 19.5 19.6 19.6 18.7 20.8 21.1 18.8 19.2 35.4 34.3 34.2 34.9 35.1 34.9 34.5 35.0 34.5 34.2 874 1010 907 1068 806 845 1012 758 938 858 MEAN S.D. N 7.26 .695 10 14.2 1.04 10 41.0 3.11 10 56.6 2.40 10 19.7 .94 10 34.7 .42 10 908 99.0 10 Covance 6329-183 3M T-6295 1576 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 6.95 14.5 41.2 59.2 20.9 35.2 858 7.11 14.2 41.7 58.6 20.0 34.2 940 6.18 13.1 37.4 60.5 21.2 35.1 799 7.83 14.8 42.3 54.0 19.0 35.1 724 7.97 14.8 43.1 54.1 18.6 34.4 896 7.28 14.2 40.8 56.0 19.5 34.9 946 7.35 14.8 41.4 56.3 20.1 35.7 850 6.87 13.5 38.2 55.5 19.6 35.3 659 7.62 14.6 42.0 55.1 19.2 34.9 847 7.97 14.9 43.1 54.1 18.6 34.5 988 MEAN S.D. N 7.31 .566 10 14.3 .61 10 41.1 1.91 10 56.3 2.32 10 19.7 .89 10 34.9 .45 10 851 101.9 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 7.84 7.92 7.05 7.08 8.06 6.87 7.24 7.27 7.33 6.58 14.4 14.7 14.2 13.7 15.2 14.5 14.1 14.3 14.3 13.1 41.6 42.6 40.7 39.9 44.1 41.0 40.5 41.4 41.8 37.5 53.0 53.8 57.7 56.4 54.7 59.7 56.0 56.9 57.0 57.0 18.3 18.5 20.1 19.4 18.8 21.1 19.5 19.7 19.5 20.0 34.6 34.4 34.9 34.3 34.4 35.4 34.9 34.6 34.1 35.1 799 828 842 788 1042 787 883 741 774 888 MEAN S.D. N 7.32 .479 10 14.2 .56 10 41.1 1.73 10 56.2 1.96 10 19.5 .83 10 34.7 .40 10 837 85.9 10 Covance 6329-183 3M T-6295 1577 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC X106/.L -------- HGB G/DL -------- HCT % -------- MCV FL -------- MCH PG -------- MCHC % -------- PLT X103/.L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 6.23 7.17 7.17 5.39 7.58 7.64 8.64 6.68 7.34 6.66 12.0 14.3 13.6 10.5 14.7 14.1 16.6 12.4 13.9 13.1 33.9 40.2 38.3 29.9 41.0 39.8 46.8 35.3 38.9 36.5 54.5 56.1 53.4 55.4 54.1 52.0 54.2 52.8 53.0 54.7 19.2 20.0 18.9 19.4 19.4 18.5 19.2 18.6 18.9 19.6 35.3 35.6 35.4 35.0 35.9 35.5 35.4 35.2 35.7 35.9 1029 819 1053 1058 1077 849 1142 1083 1158 964 MEAN S.D. N 7.05 .881 10 13.5 1.66 10 38.1 4.56 10 54.0 1.25 10 19.2 .46 10 35.5 .29 10 1023 113.7 10 Covance 6329-183 3M T-6295 1578 ANIMAL NUMBER ------ Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 MEAN S.D. N Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 0 Dosage Unit: ppm T-6295 3.1 1.4 1.2 .4 .1 .0 46 39 13 2 0 3.6 1.0 2.2 .4 .1 .0 27 60 11 2 0 4.7 2.4 1.9 .4 .1 .0 50 41 7 2 0 3.5 1.0 1.8 .5 .1 .0 30 52 13 4 0 5.6 2.5 2.3 .8 .1 .0 44 41 14 2 0 3.1 .9 1.6 .5 .1 .0 29 52 16 2 0 2.1 .6 1.1 .3 .1 .0 30 50 16 3 0 3.0 .8 1.8 .3 .1 .0 28 60 9 3 0 4.7 1.5 2.6 .5 .1 .0 32 55 10 2 0 8.1 4.4 2.9 .7 .1 .0 55 36 8 1 0 4.2 1.6 1.9 .5 .1 .0 37 49 12 2 0 1.72 1.16 .57 .16 .00 .00 10.5 8.8 3.2 .8 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 4.3 .8 2.7 .8 .1 .0 18 62 18 2 0 3.9 .8 2.5 .6 .1 .0 20 62 15 2 0 7.9 1.4 5.8 .6 .1 .0 18 73 8 2 0 3.5 .9 2.0 .5 .0 .0 26 57 14 1 0 5.4 1.5 3.2 .5 .1 .0 28 60 10 2 0 4.6 1.0 2.6 .9 .1 .0 22 56 19 2 1 5.2 1.1 3.6 .4 .1 .0 21 69 7 3 0 2.5 .8 1.4 .3 .1 .0 30 54 12 4 0 3.5 .6 2.4 .4 .1 .0 16 69 12 3 0 6.0 1.1 4.4 .3 .1 .0 19 74 5 2 0 4.7 1.0 3.1 .5 .1 .0 22 64 12 2 0 1.53 .28 1.27 .20 .03 .00 4.7 7.2 4.6 .8 .3 10 10 10 10 10 10 10 10 10 10 10 1579 ANIMAL NUMBER ------ Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 MEAN S.D. N Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 2.0 Dosage Unit: ppm T-6295 5.2 2.1 2.3 .7 .1 .0 40 43 14 3 0 6.1 2.4 2.9 .6 .1 .0 40 48 10 2 0 7.4 1.6 5.0 .7 .2 .0 21 68 9 2 0 5.0 .9 3.5 .4 .1 .0 19 70 9 2 0 5.3 1.4 3.4 .4 .1 .0 26 64 8 3 0 4.7 1.2 2.7 .7 .2 .0 25 57 15 4 0 4.8 1.0 3.2 .5 .1 .0 21 66 10 2 0 6.7 2.0 3.9 .6 .2 .0 31 58 9 3 0 2.8 .9 1.6 .2 .1 .0 31 59 7 3 0 4.4 1.2 2.5 .5 .1 .0 28 58 11 2 0 5.2 1.5 3.1 .5 .1 .0 28 59 10 3 0 1.28 .54 .94 .16 .05 .00 7.4 8.6 2.5 .7 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 6.6 2.2 3.6 .6 .1 .0 34 55 9 2 0 4.8 1.0 3.0 .7 .1 .0 21 62 14 3 1 3.6 .9 2.4 .3 .0 .0 26 66 7 1 0 3.8 1.0 2.0 .6 .1 .0 27 53 16 3 0 3.8 .6 2.6 .4 .1 .0 16 70 11 3 0 2.5 .4 1.8 .2 .0 .0 16 74 8 2 0 5.4 1.2 3.3 .8 .1 .0 22 60 16 2 0 4.0 1.2 2.3 .5 .1 .0 29 57 13 2 0 2.7 .9 1.3 .4 .1 .0 34 48 14 3 0 5.2 2.1 2.7 .3 .1 .0 41 53 5 1 0 4.2 1.2 2.5 .5 .1 .0 27 60 11 2 0 1.27 .58 .70 .19 .04 .00 8.2 8.2 3.9 .8 .3 10 10 10 10 10 10 10 10 10 10 10 1580 ANIMAL NUMBER ------ Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 MEAN S.D. N Appendix 5 Covance 6329-183 3M T-6295 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103/.L -------- N-SEG X103/.L -------- LYMPH X103/.L -------- MONO X103/.L EOSIN X103/.L -------- -------- BASO N-SEG% X103/.L -------- -------- LYMPH% -------- MONO% -------- EOSIN% -------- BASO% -------- Dose Level: 20.0 Dosage Unit: ppm T-6295 4.6 2.0 2.0 .4 .1 .0 44 45 9 1 0 3.0 .9 1.8 .3 .1 .0 29 59 9 3 0 4.5 1.2 2.5 .6 .1 .0 27 56 14 2 0 9.9 6.6 2.3 .9 .1 .0 66 24 9 1 0 3.2 .6 2.1 .4 .1 .0 19 66 12 3 0 2.9 .5 2.1 .3 .1 .0 16 73 9 3 0 5.8 .6 4.8 .3 .1 .0 11 82 5 2 0 8.3 4.1 3.1 1.0 .1 .0 49 37 12 1 1 6.6 2.6 3.1 .8 .2 .0 39 46 12 2 0 4.9 2.2 2.0 .4 .2 .0 44 42 9 4 1 5.4 2.1 2.6 .5 .1 .0 34 53 10 2 0 2.33 1.94 .90 .27 .04 .00 17.1 17.6 2.5 1.0 .4 10 10 10 10 10 10 10 10 10 10 10 1581 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 - - - - - C92876 - - - - - C92889 - - - - - C92894 - - - - - C92899 - - - - - C92910 - - - - - C92917 - - - - - C92922 - - - - - C92923 - - - - - C92930 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 - - - - - C92938 - - - - - C92946 - - - - - C92952 - - - - - C92954 - - - - - C92958 - - - - - C92964 - - - - - C92972 - - - - - C92985 - - - - - C92990 - - - - - Covance 6329-183 3M T-6295 1582 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 - - - - - C92997 - - - - - C93012 - - - - - C93022 - - - - - C93027 - - - - - C93029 - - - - - C93032 - - - - - C93042 - - - - - C93046 - - - - - C93048 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 - - - - - C93054 - - - - - C93060 - - - - - C93078 - - - - - C93081 - - - - - C93083 - - - - - C93091 - - - - - C93095 - - - - - C93097 - - - - - C93110 - - - - - Covance 6329-183 3M T-6295 1583 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ ANISO -------- POLY -------- POIK -------- HYPO -------- TOXNEUT -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 - - - - - C93116 - - - - - C93117 - - - - - C93120 - - - - - C93130 - - - - - C93138 - - - - - C93147 - - - - - C93154 - - - - - C93163 - - - - - C93171 - - - - - Covance 6329-183 3M T-6295 1584 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 88 13 .6 5.5 4.2 1.3 .1 45 114 11 .7 5.9 3.8 2.1 .0 46 104 12 .7 6.8 4.5 2.3 .1 52 113 14 .6 6.2 4.5 1.7 .0 39 93 13 .5 6.1 4.5 1.6 .0 47 92 10 .7 5.9 4.3 1.6 .1 53 96 11 .5 6.3 4.5 1.8 .3 51 95 13 .8 6.9 4.8 2.1 .1 62 98 11 .4 6.0 4.5 1.5 .1 54 76 12 .2 5.9 4.4 1.5 .1 43 MEAN S.D. N 97 12 .6 6.2 4.4 1.8 .1 49 11.4 1.2 .18 .43 .26 .32 .09 6.6 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 98 10 .7 6.1 4.4 1.7 .2 47 97 12 .6 6.7 4.7 2.0 .1 36 91 16 .7 6.6 4.1 2.5 .0 54 91 14 .7 5.9 4.1 1.8 .2 48 93 13 .6 6.2 4.6 1.6 .0 42 104 12 .6 6.1 4.6 1.5 .2 48 91 12 .6 6.6 4.8 1.8 .0 56 105 12 .6 6.6 4.8 1.8 .1 53 98 12 .6 6.2 4.8 1.4 .2 57 99 10 .5 6.9 4.9 2.0 .0 47 MEAN S.D. N 97 12 .6 6.4 4.6 1.8 .1 49 5.2 1.8 .06 .33 .29 .31 .09 6.5 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1585 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 93 11 .5 5.9 4.4 1.5 .1 56 103 11 .7 6.5 4.9 1.6 .1 51 82 10 .6 6.6 5.0 1.6 .2 39 98 12 .7 6.3 4.7 1.6 .1 47 89 12 .9 5.8 4.5 1.3 .2 44 108 13 .7 6.2 4.3 1.9 .1 45 93 13 .5 5.7 4.2 1.5 .0 25 70 14 .5 6.0 4.3 1.7 .0 38 91 14 .4 5.8 4.3 1.5 .0 50 85 12 .6 5.7 4.2 1.5 .2 44 MEAN S.D. N 91 12 .6 6.0 4.5 1.6 .1 44 10.8 1.3 .14 .33 .29 .16 .08 8.6 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 87 15 .6 6.5 4.7 1.8 .1 67 105 13 .7 6.8 4.9 1.9 .1 53 92 12 .5 6.2 4.6 1.6 .1 36 95 16 .7 6.5 4.8 1.7 .0 35 78 13 .6 5.9 4.5 1.4 .0 55 92 13 .5 6.3 4.7 1.6 .0 22 86 10 .6 5.9 4.4 1.5 .0 35 108 10 .7 6.1 4.3 1.8 .0 53 95 10 .8 6.5 4.8 1.7 .0 70 99 13 .7 6.3 4.5 1.8 .1 52 MEAN S.D. N 94 12 .6 6.3 4.6 1.7 .0 48 8.9 2.1 .10 .29 .19 .15 .05 15.3 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1586 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 91 15 .5 6.5 4.9 1.6 .1 41 89 14 .6 6.4 4.8 1.6 .1 21 74 13 .6 6.6 4.7 1.9 .0 58 79 13 .6 6.3 4.6 1.7 .0 60 83 11 .3 6.0 4.5 1.5 .0 44 82 11 .5 6.4 4.6 1.8 .1 60 88 15 .5 6.3 4.6 1.7 .1 27 83 14 .5 5.9 4.3 1.6 .3 36 85 15 .7 6.2 4.4 1.8 .1 50 81 13 .7 6.0 4.5 1.5 .0 51 MEAN S.D. N 84 13 .6 6.3 4.6 1.7 .1 45 5.0 1.5 .12 .23 .18 .13 .09 13.7 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1587 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 107 33 104 26 104 31 72 31 153 37 157 27 122 36 125 30 146 32 134 31 0 10.3 9.3 148 5.5 105 1 11.0 10.4 150 7.1 106 1 10.9 10.5 147 6.5 103 2 11.1 10.2 145 6.4 101 0 10.3 9.7 147 6.0 102 1 10.7 10.5 146 5.7 104 4 10.4 9.5 148 6.5 104 0 11.5 10.5 150 7.7 105 0 10.3 9.1 146 5.7 104 0 10.7 9.9 148 6.0 104 MEAN S.D. N 122 26.4 10 31 3.4 10 1 1.3 10 10.7 .41 10 10.0 .54 10 148 1.6 10 6.3 .69 10 104 1.5 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 135 29 133 27 161 35 135 29 121 34 148 28 177 32 110 35 130 41 207 33 0 10.4 8.9 146 5.5 103 0 10.8 10.2 147 6.4 105 1 10.7 10.0 149 6.8 105 0 10.5 9.3 145 6.7 102 0 10.5 9.1 146 6.4 104 0 10.5 8.7 146 6.2 102 2 11.0 10.5 147 6.4 104 0 11.4 10.0 148 6.3 104 0 10.6 9.0 147 5.6 103 0 10.9 9.6 149 6.4 104 MEAN S.D. N 146 28.9 10 32 4.2 10 0 .7 10 10.7 .31 10 9.5 .62 10 147 1.3 10 6.3 .42 10 104 1.1 10 Covance 6329-183 3M T-6295 1588 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 119 28 94 24 115 30 79 22 119 40 64 28 89 23 103 30 124 32 137 28 0 10.8 9.6 148 5.5 104 0 10.8 11.0 149 7.5 103 0 10.7 10.2 148 6.6 105 0 11.4 10.3 148 6.0 106 0 10.1 9.8 145 5.4 103 4 10.9 10.1 149 6.6 105 0 10.2 10.1 148 6.6 106 1 10.5 10.2 146 5.6 105 4 10.2 9.7 148 6.5 105 1 10.3 10.1 146 5.3 104 MEAN S.D. N 104 22.6 10 28 5.2 10 1 1.6 10 10.6 .41 10 10.1 .39 10 148 1.4 10 6.2 .71 10 105 1.1 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 110 31 83 28 114 29 126 36 142 36 106 28 137 31 104 24 116 27 107 30 2 11.1 10.1 146 6.0 99 0 11.1 9.7 147 6.0 104 0 10.9 10.2 146 5.1 101 1 10.6 10.0 147 6.5 104 0 10.6 9.6 146 5.8 102 0 10.6 9.1 150 6.4 107 0 10.6 9.5 145 6.0 103 1 11.2 10.7 147 7.2 105 0 11.3 10.5 148 7.0 103 0 10.7 8.7 146 5.7 104 MEAN S.D. N 114 17.2 10 30 3.8 10 0 .7 10 10.9 .28 10 9.8 .62 10 147 1.4 10 6.2 .62 10 103 2.2 10 Covance 6329-183 3M T-6295 1589 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 122 30 170 33 121 35 143 33 95 33 118 28 145 38 137 33 129 29 133 34 1 10.5 10.1 150 6.6 104 0 10.2 8.5 146 6.0 103 0 10.6 9.9 147 6.2 104 4 10.9 9.7 146 5.4 102 0 10.2 9.6 146 5.1 102 0 11.2 10.7 148 7.0 104 0 10.9 10.4 149 6.5 103 1 10.6 9.5 148 5.6 105 0 10.7 9.6 149 6.3 103 0 10.5 9.9 147 5.9 103 MEAN S.D. N 131 19.9 10 33 3.0 10 1 1.3 10 10.6 .31 10 9.8 .59 10 148 1.4 10 6.1 .58 10 103 .9 10 Covance 6329-183 3M T-6295 1590 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 110 12 .5 6.8 5.2 1.6 .3 54 115 12 .7 6.9 5.3 1.6 .0 89 105 14 .7 6.9 5.2 1.7 .2 79 111 12 .7 7.0 5.1 1.9 .0 74 106 14 .6 7.1 5.0 2.1 .1 87 127 14 .8 7.1 5.2 1.9 .0 76 118 16 .5 6.7 5.0 1.7 .1 75 123 14 .7 7.1 5.2 1.9 .2 66 111 17 .7 6.9 5.2 1.7 .1 58 112 13 .7 6.8 5.0 1.8 .1 65 MEAN S.D. N 114 14 .7 6.9 5.1 1.8 .1 72 7.1 1.7 .10 .14 .11 .16 .10 11.5 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 112 15 .9 7.0 5.1 1.9 .1 82 102 13 .7 6.9 4.8 2.1 .0 65 102 12 .7 7.0 4.8 2.2 .0 85 116 15 .8 6.6 4.9 1.7 .1 80 123 14 .6 6.7 5.2 1.5 .0 27 116 15 .8 7.0 5.4 1.6 .1 49 106 16 .8 6.8 5.1 1.7 .2 124 107 11 .7 6.6 5.0 1.6 .0 58 118 11 .8 6.7 5.1 1.6 .0 68 111 12 .9 6.8 5.0 1.8 .0 58 MEAN S.D. N 111 13 .8 6.8 5.0 1.8 .0 70 7.0 1.8 .09 .16 .18 .23 .07 25.8 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1591 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 106 13 .7 6.8 5.0 1.8 .2 75 107 16 .7 6.6 4.9 1.7 .0 41 143 15 .9 7.0 5.1 1.9 .0 60 149 16 .7 7.0 5.1 1.9 .0 61 99 12 .7 6.5 5.0 1.5 .1 32 101 13 .7 7.6 5.8 1.8 .1 86 109 15 .9 6.8 5.0 1.8 .0 45 98 12 .5 6.3 5.0 1.3 .1 79 109 17 .8 6.4 4.6 1.8 .0 73 109 13 .9 7.1 5.4 1.7 .0 55 MEAN S.D. N 113 14 .8 6.8 5.1 1.7 .0 61 17.9 1.8 .13 .39 .32 .19 .07 17.7 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 126 10 .8 7.3 5.2 2.1 .0 54 114 12 .9 7.6 5.7 1.9 .1 59 105 12 .7 6.8 5.1 1.7 .0 49 125 14 .7 6.9 5.2 1.7 .0 82 105 13 .7 7.1 5.1 2.0 .1 42 110 12 .7 7.6 5.9 1.7 .0 78 92 14 .6 7.1 5.1 2.0 .0 58 102 13 .7 7.2 5.3 1.9 .0 53 115 13 .8 6.8 5.1 1.7 .0 68 98 13 .7 6.5 4.9 1.6 .0 35 MEAN S.D. N 109 13 .7 7.1 5.3 1.8 .0 58 11.0 1.2 .08 .35 .31 .17 .04 14.8 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1592 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 111 15 .8 7.4 5.4 2.0 .1 38 116 13 .6 7.0 5.2 1.8 .0 52 113 16 .6 7.2 5.4 1.8 .0 50 113 14 .8 6.5 5.0 1.5 .1 52 100 12 .6 6.3 4.9 1.4 .3 65 105 14 .6 6.6 5.1 1.5 .0 54 100 11 .6 7.0 5.3 1.7 .0 38 97 14 .6 6.5 5.0 1.5 .0 64 118 13 .8 6.8 5.1 1.7 .0 59 100 17 .6 7.0 5.4 1.6 .0 70 MEAN S.D. N 107 14 .7 6.8 5.2 1.6 .0 54 7.7 1.8 .10 .35 .19 .18 .10 10.7 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1593 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 148 28 143 27 121 29 99 40 108 33 104 28 143 29 96 38 93 36 177 36 0 10.6 8.3 146 6.0 106 0 10.4 7.7 145 5.2 104 0 10.9 8.2 146 6.1 102 1 10.5 7.6 145 5.3 105 1 10.6 7.3 144 5.5 104 2 11.1 8.6 146 6.6 103 0 10.9 10.5 147 6.2 105 0 11.1 8.8 145 5.9 104 0 11.2 8.8 144 6.0 104 0 10.2 8.8 146 6.1 106 MEAN S.D. N 123 28.2 10 32 4.8 10 0 .7 10 10.8 .34 10 8.5 .90 10 145 1.0 10 5.9 .43 10 104 1.3 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 199 34 172 44 124 30 120 32 99 23 104 26 103 27 106 31 86 30 86 30 0 10.4 8.3 144 5.3 104 4 10.3 8.4 146 5.9 105 2 10.6 9.1 146 6.3 104 0 10.5 8.0 146 6.0 104 1 10.5 6.7 145 5.8 105 0 11.2 7.1 145 6.1 103 0 11.0 9.2 145 6.7 103 2 10.3 10.1 147 6.8 105 2 10.9 7.8 145 6.5 104 0 10.5 9.1 145 6.0 106 MEAN S.D. N 120 37.2 10 31 5.6 10 1 1.4 10 10.6 .31 10 8.4 1.03 10 145 .8 10 6.1 .45 10 104 .9 10 Covance 6329-183 3M T-6295 1594 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 96 31 96 19 115 34 86 24 104 25 124 28 94 31 85 26 104 36 104 36 5 11.1 8.8 145 6.2 104 1 10.5 10.4 147 6.1 105 0 10.6 7.1 147 6.6 105 0 10.2 7.2 146 5.8 105 4 10.4 8.9 148 6.7 107 4 11.4 9.4 149 7.2 105 1 10.9 8.4 147 6.1 104 4 10.5 8.5 147 5.5 107 0 10.4 7.1 145 5.7 106 1 10.9 9.0 148 6.1 107 MEAN S.D. N 101 12.2 10 29 5.6 10 2 2.0 10 10.7 .37 10 8.5 1.08 10 147 1.3 10 6.2 .51 10 106 1.2 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 158 30 92 33 98 41 114 37 143 24 78 26 110 33 114 35 97 29 120 33 4 10.2 7.0 146 5.6 103 1 10.8 7.5 150 6.2 105 2 10.6 8.5 147 6.4 107 1 10.8 7.3 146 5.5 104 1 10.4 9.6 150 6.1 107 0 11.5 7.7 145 6.2 104 3 10.9 8.9 148 6.7 105 1 10.8 7.1 146 5.8 105 0 10.9 7.8 145 6.5 103 2 10.5 8.0 143 5.7 103 MEAN S.D. N 112 23.9 10 32 5.1 10 2 1.3 10 10.7 .35 10 7.9 .83 10 147 2.2 10 6.1 .41 10 105 1.5 10 Covance 6329-183 3M T-6295 1595 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 100 30 129 48 76 36 90 26 83 39 73 25 86 31 92 31 91 31 103 26 0 10.7 11.1 150 6.2 107 0 10.3 6.4 147 5.6 105 0 10.6 6.9 146 5.2 105 0 10.9 10.0 148 7.2 105 0 10.6 8.0 144 5.9 106 2 10.6 7.8 145 5.7 105 3 10.6 7.9 145 6.0 104 0 10.3 8.0 145 5.9 105 1 10.6 6.7 146 5.6 104 0 11.1 8.7 145 6.5 103 MEAN S.D. N 92 16.0 10 32 7.1 10 1 1.1 10 10.6 .24 10 8.2 1.47 10 146 1.8 10 6.0 .56 10 105 1.1 10 Covance 6329-183 3M T-6295 1596 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92506 11 C92524 8 C92526 12 C92529 8 C92546 7 MEAN 9 S.D. 2.2 N 5 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92574 8 C92575 12 C92610 10 C92613 10 C92618 6 MEAN 9 S.D. 2.3 N 5 Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92646 7 C92650 3 C92652 4 C92668 12 C92678 11 MEAN 7 S.D. 4.0 N 5 Covance 6329-183 3M T-6295 1597 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92715 9 C92718 7 C92731 7 C92734 8 C92744 8 MEAN 8 S.D. .8 N 5 Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 4 C92759 6 C92779 6 C92793 8 C92798 6 MEAN 6 S.D. 1.4 N 5 Covance 6329-183 3M T-6295 1598 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 4 C92864 4 C92914 5 C92919 7 C92926 3 MEAN 5 S.D. 1.5 N 5 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 5 C92945 4 C92950 6 C92969 6 C92982 7 MEAN 6 S.D. 1.1 N 5 Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92996 3 C93006 1 C93008 3 C93031 5 C93045 1 MEAN 3 S.D. 1.7 N 5 Covance 6329-183 3M T-6295 1599 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 4 C93067 2 C93070 1 C93075 2 C93084 2 MEAN 2 S.D. 1.1 N 5 Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 4 C93123 3 C93142 5 C93144 4 C93159 6 MEAN 4 S.D. 1.1 N 5 Covance 6329-183 3M T-6295 1600 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 106 10 .4 6.7 4.5 2.2 .0 66 108 12 .6 6.9 4.8 2.1 .0 56 91 11 .4 7.2 5.2 2.0 .0 64 108 14 .7 7.1 5.1 2.0 .1 47 103 11 .3 6.6 4.8 1.8 .3 53 101 12 .4 7.4 5.2 2.2 .1 80 109 14 .6 7.6 5.0 2.6 .2 74 97 16 .4 6.6 4.5 2.1 .1 42 101 14 .3 6.5 5.0 1.5 .6 81 92 13 .3 6.4 4.7 1.7 .2 68 MEAN S.D. N 102 13 .4 6.9 4.9 2.0 .2 63 6.5 1.8 .14 .41 .26 .30 .18 13.4 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 92 14 .6 7.2 5.0 2.2 .3 52 117 17 .8 7.4 5.4 2.0 .0 52 116 12 .6 7.2 4.9 2.3 .2 65 87 14 .6 7.2 5.1 2.1 .3 51 108 15 .3 6.9 4.9 2.0 .2 40 99 14 .7 6.6 5.1 1.5 .1 33 117 13 .6 7.1 5.0 2.1 .0 91 104 11 .6 6.7 5.0 1.7 .0 41 101 14 .5 7.1 4.9 2.2 .0 42 118 13 .4 6.3 4.6 1.7 .4 60 MEAN S.D. N 106 14 .6 7.0 5.0 2.0 .2 53 11.2 1.6 .14 .34 .20 .26 .15 16.6 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1601 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 99 12 .4 6.7 4.6 2.1 .3 65 73 16 .6 6.6 4.7 1.9 .0 40 99 17 .4 6.3 4.5 1.8 .3 32 99 13 .5 7.4 5.0 2.4 .0 76 106 13 .6 7.2 4.8 2.4 .1 50 99 11 .4 6.5 4.6 1.9 .2 66 79 14 .6 6.6 4.9 1.7 .2 50 65 14 .7 6.4 4.7 1.7 .0 34 94 13 .5 6.7 4.7 2.0 .1 42 99 11 .5 6.8 4.9 1.9 .0 51 MEAN S.D. N 91 13 .5 6.7 4.7 2.0 .1 51 13.7 2.0 .10 .34 .16 .25 .12 14.5 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 105 18 .4 6.7 4.7 2.0 .2 48 105 14 .8 8.0 5.5 2.5 .1 52 89 14 .6 6.9 4.9 2.0 .1 53 110 13 .6 6.7 4.8 1.9 .5 58 84 14 .5 6.8 4.7 2.1 .2 59 90 14 .6 6.9 4.9 2.0 .4 70 107 15 .5 6.8 4.7 2.1 .1 53 98 14 .4 7.6 5.3 2.3 .0 50 98 13 .5 6.5 4.6 1.9 .3 63 106 14 .6 7.2 4.9 2.3 .3 63 MEAN S.D. N 99 14 .6 7.0 4.9 2.1 .2 57 8.9 1.4 .12 .46 .29 .20 .15 6.9 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1602 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 100 19 .6 6.4 4.5 1.9 .2 41 85 17 .8 6.8 4.9 1.9 .0 39 105 14 .5 7.0 4.8 2.2 .3 27 95 16 .5 6.5 4.7 1.8 .0 37 74 16 .7 6.9 5.1 1.8 .0 30 91 15 .6 7.2 5.0 2.2 .3 45 96 18 .6 6.9 4.9 2.0 .1 18 111 17 .7 7.4 5.1 2.3 .0 29 96 16 .6 7.4 5.3 2.1 .1 66 98 14 .2 6.8 4.7 2.1 .3 37 MEAN S.D. N 95 16 .6 6.9 4.9 2.0 .1 37 10.3 1.6 .16 .34 .24 .18 .13 12.9 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1603 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 116 31 73 36 149 33 128 33 122 28 83 37 110 49 89 37 125 28 116 46 1 10.1 7.3 147 6.7 106 1 11.1 8.4 147 6.2 106 0 10.6 7.5 145 6.1 103 0 10.5 7.4 146 6.3 107 4 10.2 8.1 146 6.1 104 0 10.7 7.1 146 6.6 105 0 10.4 6.3 145 5.5 104 4 10.4 7.5 146 5.7 105 1 10.5 7.8 146 6.3 106 1 10.4 7.7 146 6.0 104 MEAN S.D. N 111 23.1 10 36 7.0 10 1 1.5 10 10.5 .28 10 7.5 .57 10 146 .7 10 6.2 .37 10 105 1.2 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 123 40 115 48 92 34 104 40 122 51 114 43 140 32 102 36 131 48 77 39 1 10.4 7.9 145 6.5 102 1 11.0 8.5 154 7.3 108 1 11.0 8.3 147 6.6 102 0 10.6 8.9 146 6.6 103 1 10.2 7.6 146 6.4 104 2 10.6 7.2 146 6.3 107 0 10.5 7.0 146 6.4 105 1 10.1 7.4 146 6.3 105 0 10.5 7.1 146 6.2 106 0 10.4 7.6 145 6.2 104 MEAN S.D. N 112 18.8 10 41 6.3 10 1 .7 10 10.5 .29 10 7.8 .64 10 147 2.6 10 6.5 .32 10 105 2.0 10 Covance 6329-183 3M T-6295 1604 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 118 42 153 37 141 39 121 33 89 44 112 36 142 47 130 39 109 46 151 45 0 10.5 7.9 146 7.0 104 0 10.6 8.2 146 6.2 102 3 10.3 8.5 147 6.9 103 3 10.6 7.8 147 7.1 103 1 10.5 7.6 147 6.7 105 0 10.3 7.3 146 6.0 106 0 10.5 7.9 147 5.8 105 0 10.0 8.7 147 6.1 104 2 10.6 7.3 146 6.1 105 0 10.2 6.5 152 6.3 110 MEAN S.D. N 127 20.5 10 41 4.7 10 1 1.3 10 10.4 .20 10 7.8 .64 10 147 1.8 10 6.4 .46 10 105 2.2 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 117 38 130 47 145 34 104 29 124 43 147 71 143 34 141 62 134 46 109 32 2 10.4 7.7 146 6.3 104 0 11.0 7.1 153 6.5 108 0 10.6 7.8 146 6.0 101 0 10.4 7.5 144 5.5 104 0 10.5 8.3 146 6.3 104 0 10.3 7.1 147 5.7 103 1 10.2 7.0 144 5.9 102 0 10.9 8.4 150 6.7 102 1 10.0 7.2 145 6.1 102 5 10.3 6.7 147 5.9 105 MEAN S.D. N 129 15.4 10 44 13.6 10 1 1.6 10 10.5 .31 10 7.5 .57 10 147 2.8 10 6.1 .37 10 104 2.0 10 Covance 6329-183 3M T-6295 1605 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 113 67 293 213 100 57 111 37 140 40 79 38 108 57 131 52 117 44 88 46 0 10.2 8.1 145 6.1 102 0 10.6 8.1 147 6.2 104 5 10.7 7.8 144 6.4 104 1 10.6 8.3 147 7.0 103 0 10.1 7.1 147 5.9 105 0 10.5 7.3 145 5.6 104 0 10.4 6.8 147 6.7 105 0 10.7 7.9 147 6.3 102 0 10.8 8.0 145 6.4 102 0 10.6 8.7 148 7.4 106 MEAN S.D. N 128 60.7 10 65 52.9 10 1 1.6 10 10.5 .23 10 7.8 .58 10 146 1.3 10 6.4 .52 10 104 1.4 10 Covance 6329-183 3M T-6295 1606 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 109 14 .7 7.6 5.5 2.1 .0 89 108 16 .5 7.4 5.2 2.2 .3 63 113 16 .8 8.4 5.8 2.6 .0 63 112 13 .6 7.5 5.4 2.1 .0 56 112 14 .6 8.0 5.9 2.1 .2 72 113 14 .5 7.3 5.3 2.0 .1 107 94 16 .8 7.3 5.3 2.0 .0 83 77 15 .7 7.7 5.5 2.2 .1 72 103 19 .5 7.6 5.8 1.8 .6 70 117 16 .6 7.7 5.8 1.9 .1 71 MEAN S.D. N 106 15 .6 7.6 5.6 2.1 .1 75 12.0 1.7 .12 .34 .25 .22 .19 14.9 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 107 13 .6 8.4 6.5 1.9 .1 81 113 15 .8 8.1 5.9 2.2 .4 69 88 15 .6 7.8 5.7 2.1 .1 65 106 17 .8 8.2 5.8 2.4 .2 101 109 9 .4 8.0 6.2 1.8 .3 63 112 21 .7 7.5 5.7 1.8 .4 74 110 15 .6 8.4 6.5 1.9 .0 80 92 12 .5 8.6 6.6 2.0 .1 98 117 15 .7 8.2 6.0 2.2 .2 94 105 16 .6 9.0 7.0 2.0 .3 155 MEAN S.D. N 106 15 .6 8.2 6.2 2.0 .2 88 9.1 3.2 .13 .42 .44 .19 .14 27.1 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1607 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 106 14 .7 7.8 5.7 2.1 .2 71 101 15 .7 7.7 5.8 1.9 .2 63 105 15 .8 7.8 5.7 2.1 .0 63 109 14 .8 7.8 6.1 1.7 .1 113 108 16 .7 7.9 6.2 1.7 .1 106 109 14 .7 7.5 5.5 2.0 .0 68 122 19 1.0 8.3 6.2 2.1 .1 98 113 18 .9 8.5 6.1 2.4 .0 131 108 16 .6 7.7 5.8 1.9 .1 71 103 11 .9 8.1 5.9 2.2 .0 88 MEAN S.D. N 108 15 .8 7.9 5.9 2.0 .1 87 5.9 2.3 .12 .30 .24 .22 .08 23.9 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 85 19 .7 8.0 5.9 2.1 .1 54 103 15 .8 8.7 6.4 2.3 .1 76 87 16 .5 8.0 6.3 1.7 .2 94 96 12 .6 7.8 5.3 2.5 .1 71 92 14 .3 7.4 5.2 2.2 .5 82 85 17 .6 7.8 6.0 1.8 .1 47 105 13 .7 8.6 6.1 2.5 .2 62 108 14 .6 7.4 5.7 1.7 .1 70 96 16 .5 7.9 5.5 2.4 .2 73 91 15 .7 8.3 6.3 2.0 .4 70 MEAN S.D. N 95 15 .6 8.0 5.9 2.1 .2 70 8.3 2.0 .14 .44 .43 .31 .14 13.4 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1608 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 104 14 .6 8.0 6.0 2.0 .0 44 102 19 .6 7.6 5.9 1.7 .4 67 92 20 .7 7.9 6.0 1.9 .1 45 87 15 .6 7.7 5.9 1.8 .2 69 94 18 .7 8.0 5.9 2.1 .3 76 104 18 .7 8.6 6.4 2.2 .1 88 103 18 .6 7.4 5.8 1.6 .2 56 93 19 .6 8.0 6.0 2.0 .2 73 102 17 .6 7.9 6.2 1.7 .0 69 107 23 .7 8.9 6.9 2.0 .4 75 MEAN S.D. N 99 18 .6 8.0 6.1 1.9 .2 66 6.7 2.5 .05 .45 .33 .19 .14 14.0 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1609 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 89 29 110 35 115 36 100 37 94 33 77 31 133 34 113 34 107 36 88 33 3 11.0 6.3 150 7.0 106 2 10.3 5.4 147 5.6 108 3 10.7 6.0 149 6.3 106 0 10.6 6.4 151 5.8 107 0 10.6 4.6 149 5.7 107 1 11.7 7.9 146 6.2 107 0 10.1 7.4 145 6.0 108 0 10.3 6.8 146 5.6 105 1 10.7 6.4 146 6.0 107 1 10.8 5.8 147 6.3 107 MEAN S.D. N 103 16.3 10 34 2.4 10 1 1.2 10 10.7 .45 10 6.3 .95 10 148 2.0 10 6.0 .43 10 107 .9 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 87 28 132 36 140 32 113 23 95 28 97 35 108 55 88 34 100 43 113 41 4 11.3 7.4 146 6.9 103 0 10.7 7.2 149 6.7 107 0 10.4 6.1 146 6.1 105 3 11.1 7.9 149 7.4 105 1 10.5 4.5 147 5.5 106 0 11.0 5.9 149 5.9 108 0 11.0 4.0 147 5.9 106 1 11.3 6.1 147 5.5 105 4 11.3 7.4 153 7.5 108 0 12.1 7.1 149 7.1 104 MEAN S.D. N 107 17.8 10 36 9.1 10 1 1.7 10 11.1 .49 10 6.4 1.30 10 148 2.1 10 6.4 .76 10 106 1.6 10 Covance 6329-183 3M T-6295 1610 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 81 31 100 25 108 25 93 40 174 85 93 33 115 40 93 28 85 25 90 35 0 10.6 6.4 148 6.4 106 0 10.5 5.3 145 5.5 106 0 10.5 6.7 146 5.1 104 0 11.3 6.6 147 6.3 103 3 11.6 7.4 149 7.0 107 0 10.5 5.7 148 6.1 107 1 11.1 7.7 155 7.1 107 1 11.5 7.6 148 6.8 105 4 10.8 6.6 145 6.1 105 0 10.7 5.4 145 5.2 104 MEAN S.D. N 103 26.9 10 37 17.9 10 1 1.4 10 10.9 .43 10 6.5 .87 10 148 3.0 10 6.2 .71 10 105 1.4 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 144 37 92 37 105 36 90 34 90 26 91 29 101 35 102 37 100 30 115 42 0 10.7 8.1 150 6.3 108 0 11.3 6.5 150 6.6 108 2 10.7 7.4 147 5.2 106 5 10.3 6.6 145 6.1 106 0 10.4 6.3 148 6.4 108 1 10.4 8.2 145 4.6 104 1 11.3 6.6 149 6.3 107 4 9.8 4.5 146 4.7 106 4 10.7 6.5 144 6.1 104 0 11.4 5.3 153 5.9 110 MEAN S.D. N 103 16.5 10 34 4.7 10 2 1.9 10 10.7 .51 10 6.6 1.14 10 148 2.8 10 5.8 .72 10 107 1.9 10 Covance 6329-183 3M T-6295 1611 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 66 30 92 43 85 33 79 28 137 89 85 40 77 35 80 27 88 31 115 37 1 11.0 7.1 148 6.2 106 0 10.6 6.8 149 6.2 106 1 10.4 7.0 146 6.8 106 1 10.4 6.1 145 5.1 104 4 10.5 6.7 147 5.6 106 1 11.2 6.2 153 6.6 110 0 10.7 5.5 151 6.2 111 0 10.8 7.3 145 5.8 102 0 10.7 5.0 148 6.0 110 4 11.2 7.2 150 7.0 104 MEAN S.D. N 90 20.7 10 39 18.2 10 1 1.5 10 10.8 .30 10 6.5 .77 10 148 2.6 10 6.2 .57 10 106 3.0 10 Covance 6329-183 3M T-6295 1612 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92509 4 C92511 6 C92521 3 C92528 6 C92532 4 MEAN 5 S.D. 1.3 N 5 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92593 8 C92600 1 C92616 8 C92621 5 C92627 2 MEAN 5 S.D. 3.3 N 5 Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92640 8 C92645 4 C92662 8 C92676 1 C92684 6 MEAN 5 S.D. 3.0 N 5 Covance 6329-183 3M T-6295 1613 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92713 2 C92714 0 C92719 4 C92722 3 C92728 0 MEAN 2 S.D. 1.8 N 5 Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92765 4 C92777 3 C92789 8 C92799 6 C92812 6 MEAN 5 S.D. 1.9 N 5 Covance 6329-183 3M T-6295 1614 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92880 0 C92887 3 C92898 4 C92903 1 C92905 1 MEAN 2 S.D. 1.6 N 5 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92944 2 C92962 4 C92967 6 C92978 0 C92987 1 MEAN 3 S.D. 2.4 N 5 Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 2 C93000 1 C93018 1 C93023 0 C93035 1 MEAN 1 S.D. .7 N 5 Covance 6329-183 3M T-6295 1615 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ PCOAO IU/G -------- Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 0 C93064 0 C93071 6 C93085 2 C93109 0 MEAN 2 S.D. 2.6 N 5 Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 5 C93127 7 C93143 6 C93155 0 C93169 7 MEAN 5 S.D. 2.9 N 5 Covance 6329-183 3M T-6295 1616 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 107 16 .6 6.5 4.4 2.1 .2 55 125 11 .8 6.8 4.6 2.2 .1 70 119 12 .6 7.8 4.9 2.9 .1 91 126 16 .7 7.4 4.8 2.6 .3 82 120 14 .8 7.1 5.3 1.8 .4 110 105 11 .9 7.3 4.9 2.4 .3 87 107 10 .7 7.1 4.8 2.3 .2 66 121 10 .7 7.8 5.1 2.7 .4 131 108 11 .6 7.7 5.2 2.5 .2 120 111 11 .8 7.4 4.6 2.8 .1 74 MEAN S.D. N 115 12 .7 7.3 4.9 2.4 .2 89 8.1 2.3 .10 .43 .28 .34 .12 24.7 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 126 15 .7 7.0 4.9 2.1 .3 74 103 12 .8 7.1 4.8 2.3 .4 63 125 15 .8 6.6 4.3 2.3 .3 93 113 10 .6 7.2 5.1 2.1 .3 55 102 12 .8 6.7 4.4 2.3 .3 86 131 12 .8 7.2 4.8 2.4 .3 76 93 11 .7 6.5 4.4 2.1 .2 67 118 12 .7 7.4 5.1 2.3 .4 50 141 14 1.1 8.2 5.0 3.2 .3 148 119 14 .5 7.2 4.6 2.6 .3 129 MEAN S.D. N 117 13 .8 7.1 4.7 2.4 .3 84 14.7 1.7 .16 .48 .30 .33 .06 31.8 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1617 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 129 13 .9 7.4 4.6 2.8 .2 89 103 18 .7 6.9 4.7 2.2 .5 44 115 14 .8 7.2 5.0 2.2 .4 63 119 12 .7 7.4 4.9 2.5 .6 86 97 12 .8 7.2 4.7 2.5 .2 70 136 13 .8 7.5 4.7 2.8 .4 84 104 10 .6 7.2 4.8 2.4 .5 86 103 10 .8 6.8 4.7 2.1 .5 65 110 14 .7 6.9 4.8 2.1 .2 64 128 12 .7 7.7 5.4 2.3 .2 92 MEAN S.D. N 114 13 .8 7.2 4.8 2.4 .4 74 13.2 2.3 .08 .29 .23 .26 .16 15.5 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 98 14 .8 7.5 5.1 2.4 .3 47 91 11 .6 7.0 4.7 2.3 .4 47 113 15 .7 7.6 4.9 2.7 .2 60 118 10 .5 7.2 4.6 2.6 .1 139 116 19 1.0 7.2 4.7 2.5 .2 67 109 15 .8 7.0 4.8 2.2 .1 71 121 13 .9 8.2 5.5 2.7 .4 81 124 12 .5 7.5 4.9 2.6 .4 80 110 14 .7 6.7 4.7 2.0 .3 43 130 13 .8 7.9 5.2 2.7 .3 89 MEAN S.D. N 113 14 .7 7.4 4.9 2.5 .3 72 11.7 2.5 .16 .45 .28 .24 .12 28.2 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1618 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 120 17 .7 7.2 4.9 2.3 .1 46 86 19 1.1 8.1 5.5 2.6 .2 36 112 19 .6 7.2 4.7 2.5 .2 38 103 17 .7 7.8 5.2 2.6 .1 28 117 13 .7 7.8 5.0 2.8 .4 65 118 16 .9 7.1 4.9 2.2 .1 45 114 19 .8 7.9 5.5 2.4 .3 61 114 20 .9 7.5 5.1 2.4 .3 44 132 16 .9 7.3 5.1 2.2 .3 57 98 16 .9 7.5 5.1 2.4 .2 43 MEAN S.D. N 111 17 .8 7.5 5.1 2.4 .2 46 12.8 2.1 .15 .34 .25 .19 .10 11.6 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1619 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 80 33 61 40 101 34 110 43 74 25 74 33 108 40 75 40 64 33 94 46 0 10.6 6.8 146 6.2 105 2 10.7 6.8 148 6.7 107 0 11.4 6.9 146 7.0 104 0 11.1 7.0 145 6.9 101 1 11.1 6.9 144 6.7 105 2 10.9 6.4 146 6.4 105 1 10.7 7.0 147 6.6 104 3 11.4 5.9 146 6.7 101 4 11.7 6.4 147 6.6 106 1 11.2 6.9 146 6.8 103 MEAN S.D. N 84 17.8 10 37 6.2 10 1 1.3 10 11.1 .36 10 6.7 .36 10 146 1.1 10 6.7 .23 10 104 2.0 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 66 34 79 42 85 31 83 35 77 41 80 28 64 33 81 43 81 30 112 45 0 11.3 7.0 148 6.8 106 1 11.0 7.3 145 6.8 104 4 10.5 5.8 143 6.7 103 4 10.8 6.5 147 7.0 105 3 11.1 6.7 146 6.4 105 1 10.9 6.6 146 7.0 103 0 10.8 7.0 146 6.5 105 0 11.4 6.5 149 7.3 107 4 12.0 7.7 148 7.8 103 3 11.5 7.7 148 8.0 103 MEAN S.D. N 81 13.0 10 36 6.1 10 2 1.8 10 11.1 .43 10 6.9 .59 10 147 1.8 10 7.0 .53 10 104 1.4 10 Covance 6329-183 3M T-6295 1620 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 71 39 124 37 70 53 103 60 104 44 65 40 128 54 88 42 88 34 82 65 1 11.4 7.0 148 7.1 104 1 10.8 7.2 149 7.3 107 1 11.0 6.7 146 6.3 106 1 11.3 7.3 146 7.2 104 0 10.9 7.0 147 7.3 103 2 11.5 6.7 150 7.1 105 1 10.8 6.4 147 6.4 104 1 10.9 6.8 144 6.3 102 0 10.9 6.8 145 5.8 104 0 11.4 6.4 145 6.3 103 MEAN S.D. N 92 22.0 10 47 10.5 10 1 .6 10 11.1 .28 10 6.8 .30 10 147 1.9 10 6.7 .54 10 104 1.5 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 85 42 92 36 89 47 71 30 92 41 82 34 76 45 69 34 83 28 65 40 1 11.1 6.8 149 6.7 106 0 10.8 6.8 148 6.7 104 0 11.6 8.3 150 7.5 105 1 11.8 6.9 146 7.0 104 0 11.0 7.1 147 6.8 106 3 11.3 6.6 147 7.0 106 4 12.2 8.2 151 7.5 103 0 10.9 6.8 146 6.4 102 2 10.3 6.6 145 6.0 104 1 11.8 6.9 149 7.1 105 MEAN S.D. N 80 9.7 10 38 6.3 10 1 1.4 10 11.3 .57 10 7.1 .62 10 148 1.9 10 6.9 .46 10 104 1.4 10 Covance 6329-183 3M T-6295 1621 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 82 37 139 64 127 75 72 37 63 38 233 194 61 44 108 83 62 37 79 38 2 10.7 7.3 145 6.5 103 0 11.7 7.9 149 7.7 105 1 11.1 7.7 147 7.5 105 0 11.0 6.8 146 6.9 104 1 11.6 6.7 145 6.4 105 6 10.9 7.6 146 6.5 106 0 11.0 5.4 144 6.0 102 0 11.6 8.6 152 7.8 107 1 11.0 6.6 144 6.2 104 3 11.3 8.1 148 7.2 104 MEAN S.D. N 103 53.6 10 65 48.7 10 1 1.9 10 11.2 .34 10 7.3 .92 10 147 2.5 10 6.9 .65 10 104 1.4 10 Covance 6329-183 3M T-6295 1622 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 92 15 1.1 8.8 6.4 2.4 .4 139 115 12 1.1 8.7 6.4 2.3 .1 82 101 13 .8 8.9 6.3 2.6 .5 79 104 15 .7 8.7 6.4 2.3 .2 100 97 16 1.1 8.3 5.6 2.7 .3 149 107 14 .9 9.6 6.8 2.8 .3 128 102 12 .8 8.6 6.1 2.5 .4 129 114 14 .9 9.3 7.1 2.2 .4 133 99 14 .9 8.9 6.4 2.5 .4 129 108 14 .6 9.1 6.8 2.3 .6 86 MEAN S.D. N 104 14 .9 8.9 6.4 2.5 7.3 1.3 .17 .37 .41 .20 10 10 10 10 10 10 .4 115 .14 26.0 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 89 18 1.1 7.9 6.2 1.7 .2 137 104 12 .6 9.3 7.1 2.2 .4 80 89 15 1.0 9.0 6.2 2.8 .4 110 102 17 .9 7.4 5.3 2.1 .3 73 101 16 .9 7.7 5.4 2.3 .4 92 106 13 .7 8.9 6.8 2.1 .1 68 99 16 1.2 8.0 5.8 2.2 .3 82 93 12 1.1 8.2 5.8 2.4 .3 72 92 14 .7 8.9 6.6 2.3 .4 87 101 13 1.1 9.0 6.5 2.5 .3 153 MEAN S.D. N 98 15 .9 8.4 6.2 2.3 .3 95 6.3 2.1 .21 .66 .59 .29 .10 29.0 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1623 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 99 14 .8 7.7 5.2 2.5 .1 60 93 15 .9 8.0 5.4 2.6 .5 70 92 11 1.0 9.0 6.2 2.8 .2 82 97 14 .9 8.1 5.6 2.5 .4 56 102 17 1.2 8.4 5.9 2.5 .1 87 100 18 1.1 9.9 7.2 2.7 .3 94 108 16 1.1 8.9 6.9 2.0 .4 107 108 15 1.1 8.5 6.1 2.4 .2 76 106 14 .9 7.9 5.8 2.1 .2 64 82 16 .7 7.6 5.4 2.2 .2 89 MEAN S.D. N 99 15 1.0 8.4 6.0 2.4 .3 78 8.2 1.9 .16 .71 .65 .26 .13 16.3 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 112 14 1.1 8.4 6.2 2.2 .1 84 86 20 .8 8.4 6.3 2.1 .1 63 99 15 1.1 8.5 6.0 2.5 .2 80 111 11 .9 8.6 6.2 2.4 .2 86 89 14 1.0 8.2 5.7 2.5 .3 68 94 17 .9 8.2 6.1 2.1 .3 76 96 17 .7 7.9 5.7 2.2 .4 72 88 14 .7 8.2 6.0 2.2 .2 70 98 17 .9 7.4 5.4 2.0 .2 95 112 9 1.0 8.8 5.8 3.0 .6 94 MEAN S.D. N 98 15 .9 8.3 5.9 2.3 .3 79 10.0 3.2 .14 .39 .28 .29 .15 10.9 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1624 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 100 22 1.0 9.2 6.8 2.4 .5 75 89 16 .7 9.5 6.5 3.0 .6 90 89 19 1.0 8.5 6.6 1.9 .4 73 92 17 .7 8.1 6.0 2.1 .4 55 94 20 .7 9.0 6.6 2.4 .2 72 96 21 .9 8.1 6.0 2.1 .4 75 100 19 .8 8.1 6.0 2.1 .2 70 104 18 .9 9.0 6.4 2.6 .2 145 98 26 1.0 9.4 7.1 2.3 .2 70 81 20 .9 8.4 6.2 2.2 .4 75 MEAN S.D. N 94 20 .9 8.7 6.4 2.3 .4 80 6.8 2.8 .13 .55 .37 .31 .14 24.4 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1625 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 321 194 106 49 113 96 82 32 94 36 84 44 92 36 102 49 107 67 85 39 3 12.0 7.5 146 6.3 103 0 11.2 6.4 148 5.3 109 5 12.0 8.1 150 6.9 106 4 11.3 5.4 145 4.8 106 0 12.1 6.8 149 6.5 104 0 12.1 6.0 145 5.5 104 1 11.9 7.1 148 6.1 105 4 11.4 6.2 147 4.8 104 1 11.8 6.6 150 6.2 105 0 11.6 6.8 151 6.0 110 MEAN S.D. N 119 71.9 10 64 49.4 10 2 2.0 10 11.7 .34 10 6.7 .77 10 148 2.1 10 5.8 .71 10 106 2.3 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 128 31 79 30 104 30 97 27 85 34 79 34 97 28 130 26 118 54 102 41 0 12.7 5.7 145 4.9 107 1 11.8 6.8 146 6.9 104 4 11.7 7.9 152 5.8 106 3 10.6 6.4 147 5.5 111 1 11.4 6.2 144 5.9 105 0 11.9 6.3 147 6.1 105 2 11.2 5.8 146 5.9 107 1 11.1 7.2 147 6.6 107 1 12.7 7.1 145 6.5 105 4 11.5 5.6 148 4.8 106 MEAN S.D. N 102 18.6 10 34 8.4 10 2 1.5 10 11.7 .67 10 6.5 .74 10 147 2.2 10 5.9 .69 10 106 1.9 10 Covance 6329-183 3M T-6295 1626 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 100 30 468 448 77 30 97 33 104 54 87 32 130 49 110 33 160 29 93 34 3 10.9 6.9 146 6.1 107 6 10.9 8.0 146 5.6 105 3 11.4 5.6 148 5.9 107 1 10.9 6.0 147 5.3 110 1 11.8 7.7 154 7.1 110 1 11.9 6.0 148 5.4 107 0 11.2 4.8 147 5.1 105 4 11.0 6.0 149 5.7 108 0 11.0 6.8 147 6.2 107 0 10.9 6.5 146 5.9 106 MEAN S.D. N 143 116.7 10 77 130.6 10 2 2.0 10 11.2 .38 10 6.4 .96 10 148 2.4 10 5.8 .57 10 107 1.8 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 97 28 105 36 86 34 96 46 104 33 100 40 185 115 85 33 195 63 119 42 4 11.9 7.2 151 7.0 112 4 11.3 6.4 146 6.1 107 3 11.7 6.6 149 6.9 109 4 11.0 5.7 148 5.9 108 3 11.1 8.2 149 6.2 108 4 10.8 6.9 145 5.1 106 7 11.4 6.9 145 6.0 106 4 11.0 7.2 148 5.9 109 4 10.8 5.7 147 5.5 108 0 11.3 6.4 147 5.2 105 MEAN S.D. N 117 39.6 10 47 25.8 10 4 1.7 10 11.2 .37 10 6.7 .75 10 148 1.9 10 6.0 .63 10 108 2.0 10 Covance 6329-183 3M T-6295 1627 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 79 36 167 130 116 30 60 24 97 58 83 32 95 42 274 196 80 37 115 51 0 11.3 6.9 150 6.2 107 1 12.0 6.7 146 6.0 105 0 11.3 8.2 147 5.9 106 0 11.1 6.3 145 5.3 105 2 11.3 7.0 153 5.8 109 0 10.9 6.5 147 5.6 108 1 11.0 6.9 149 5.3 109 7 11.7 6.5 144 5.5 103 4 11.8 7.4 147 6.8 105 6 12.2 10.3 152 7.4 108 MEAN S.D. N 117 62.6 10 64 55.5 10 2 2.6 10 11.5 .44 10 7.3 1.20 10 148 2.9 10 6.0 .67 10 106 2.0 10 Covance 6329-183 3M T-6295 1628 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 123 11 .8 7.3 4.6 2.7 .0 226 117 9 .7 6.8 4.2 2.6 .0 85 127 11 .4 7.5 5.0 2.5 .0 133 127 11 .4 7.0 4.1 2.9 .0 110 154 10 .8 7.3 4.6 2.7 .1 143 137 12 .6 6.8 4.5 2.3 .0 79 128 8 .5 6.7 3.8 2.9 .0 92 147 12 .9 7.8 4.7 3.1 .0 146 116 9 .7 6.6 4.5 2.1 .2 99 115 9 .6 6.3 4.1 2.2 .0 88 MEAN S.D. N 129 10 .6 7.0 4.4 2.6 13.2 1.4 .17 .46 .35 .33 10 10 10 10 10 10 .0 120 .07 44.6 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 120 12 .7 7.4 4.7 2.7 .0 92 113 12 .7 7.6 4.5 3.1 .1 201 139 11 .8 7.3 5.0 2.3 .1 108 129 11 .6 8.0 5.4 2.6 .0 94 117 13 .7 7.4 4.8 2.6 .3 78 121 12 .7 7.4 4.7 2.7 .3 76 106 11 .6 7.1 4.8 2.3 .0 102 126 10 .8 7.4 4.9 2.5 .1 139 133 14 .9 7.7 4.6 3.1 .2 187 148 11 .7 7.2 4.6 2.6 .5 216 MEAN S.D. N 125 12 .7 7.4 4.8 2.6 12.5 1.2 .09 .26 .26 .28 10 10 10 10 10 10 .2 129 .16 53.1 10 10 Covance 6329-183 3M T-6295 1629 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 108 13 .6 7.0 4.5 2.5 .0 94 136 13 .8 7.4 5.0 2.4 .0 85 107 10 .6 7.3 4.7 2.6 .1 94 104 10 .5 6.6 4.5 2.1 .0 62 137 13 .7 7.6 4.8 2.8 .2 94 118 12 .8 7.0 4.5 2.5 .2 61 140 12 .9 8.0 4.8 3.2 .1 111 111 14 .8 7.1 4.9 2.2 .0 98 121 10 .4 7.4 4.6 2.8 .0 111 132 13 .7 7.4 5.0 2.4 .2 76 MEAN S.D. N 121 12 .7 7.3 4.7 2.6 .1 89 13.8 1.5 .15 .38 .20 .32 .09 17.7 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 116 16 .5 7.5 5.1 2.4 .1 78 122 14 .7 7.5 4.8 2.7 .3 85 117 16 .8 7.4 4.7 2.7 .1 78 126 14 .8 7.1 4.6 2.5 .0 82 130 14 .9 8.4 5.2 3.2 .1 106 130 12 .6 7.0 4.2 2.8 .0 90 115 13 .8 8.2 5.0 3.2 .0 101 107 13 .5 7.3 4.9 2.4 .0 101 131 13 1.0 7.3 4.9 2.4 .3 75 118 15 .8 7.9 4.9 3.0 .1 122 MEAN S.D. N 121 14 .7 7.6 4.8 2.7 .1 92 8.0 1.3 .16 .46 .28 .32 .12 15.2 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1630 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 109 13 .8 6.8 4.8 2.0 .0 24 108 14 .6 7.5 4.9 2.6 .0 47 94 17 .6 7.8 5.4 2.4 .0 74 116 9 .7 6.7 4.3 2.4 .0 61 117 12 .8 7.8 5.0 2.8 .0 69 107 15 .6 7.0 4.7 2.3 .1 46 127 11 .8 6.0 3.5 2.5 .0 24 115 13 .6 6.5 4.3 2.2 .0 59 97 14 .7 7.3 4.9 2.4 .0 46 MEAN S.D. N 110 13 .7 7.0 4.6 2.4 .0 50 10.2 2.3 .09 .61 .55 .23 .03 17.8 9 9 9 9 9 9 9 9 Covance 6329-183 3M T-6295 1631 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 63 26 92 35 108 38 131 32 97 29 111 38 101 26 154 241 127 41 74 37 0 11.1 6.9 150 5.8 106 0 9.9 6.0 146 5.3 107 0 10.9 6.5 148 6.1 108 0 10.2 6.1 144 5.8 105 0 10.9 6.2 147 6.3 105 0 10.3 6.7 147 6.2 108 0 10.3 6.3 145 6.7 105 0 10.7 5.8 146 6.0 103 0 10.3 5.9 146 5.5 106 0 10.2 7.0 147 5.4 109 MEAN S.D. N 106 27.0 10 54 65.8 10 0 .0 10 10.5 .39 10 6.3 .42 10 147 1.6 10 5.9 .44 10 106 1.8 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 125 58 177 58 124 70 99 47 177 205 102 30 82 45 143 41 81 26 67 44 0 11.1 6.9 149 6.5 105 0 11.4 7.8 150 7.5 107 1 11.1 6.6 149 6.7 106 0 11.4 7.0 150 6.9 106 0 11.1 7.3 148 5.8 107 1 11.3 7.3 145 5.8 108 0 11.3 6.9 151 7.0 110 0 10.8 6.8 143 6.0 103 1 12.2 7.2 149 6.5 105 0 11.9 7.2 149 6.4 106 MEAN S.D. N 118 38.8 10 62 51.8 10 0 .5 10 11.4 .41 10 7.1 .34 10 148 2.5 10 6.5 .55 10 106 1.9 10 Covance 6329-183 3M T-6295 1632 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 105 57 134 42 91 30 119 43 101 38 107 34 94 40 143 43 107 33 112 42 0 10.7 7.1 150 6.5 109 2 11.4 7.4 150 6.9 108 0 11.1 7.3 149 6.8 105 1 10.3 7.0 149 6.3 110 2 11.4 8.0 151 7.3 107 0 11.2 8.0 151 7.2 109 0 11.4 7.5 153 6.9 105 0 10.4 6.2 148 5.9 107 0 10.7 6.4 146 6.0 108 0 11.3 7.2 145 5.7 106 MEAN S.D. N 111 16.6 10 40 7.5 10 0 .8 10 11.0 .43 10 7.2 .59 10 149 2.4 10 6.6 .56 10 107 1.7 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 119 39 167 40 125 44 166 54 104 55 116 36 118 49 113 51 144 34 130 33 4 11.3 8.6 153 7.5 112 0 11.5 8.1 148 6.8 107 0 11.1 7.9 147 6.6 106 1 10.6 7.2 146 7.0 110 0 11.9 8.3 155 7.5 107 0 10.4 7.5 146 6.7 107 0 11.6 7.6 150 7.1 109 0 10.5 6.5 148 5.9 106 0 10.7 7.3 145 5.6 104 0 11.7 8.4 153 7.2 105 MEAN S.D. N 130 21.9 10 44 8.3 10 0 1.3 10 11.1 .55 10 7.7 .64 10 149 3.5 10 6.8 .63 10 107 2.4 10 Covance 6329-183 3M T-6295 1633 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 101 54 241 292 257 140 64 40 81 40 110 48 104 48 89 44 111 43 0 10.3 6.1 147 6.3 112 1 11.0 7.2 155 7.2 112 0 10.9 7.3 149 6.6 109 0 10.2 5.8 144 5.0 108 4 11.2 7.2 151 7.3 112 0 10.4 7.4 146 5.9 107 0 10.1 7.5 145 4.9 108 0 10.1 7.1 146 5.8 108 0 10.4 7.3 150 7.0 111 MEAN S.D. N 129 70.0 9 83 84.4 9 1 10.5 1.3 .41 9 9 7.0 .61 9 148 3.5 9 6.2 .89 9 110 2.1 9 Covance 6329-183 3M T-6295 1634 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 119 10 .7 9.1 6.4 2.7 .1 144 99 13 .7 7.1 5.1 2.0 .0 95 117 9 .8 6.1 4.3 1.8 .0 84 96 11 .7 7.4 5.5 1.9 .0 109 99 13 .9 7.3 5.3 2.0 .1 108 118 15 .7 8.8 6.5 2.3 .2 126 112 10 .6 9.3 6.5 2.8 .1 137 127 7 .6 8.0 6.1 1.9 .0 102 111 11 .6 6.9 4.8 2.1 .0 122 113 8 .6 7.4 4.7 2.7 .0 136 MEAN S.D. N 111 11 .7 7.7 5.5 2.2 10.1 2.5 .10 1.04 .81 .38 10 10 10 10 10 10 .0 116 .07 19.8 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 112 12 .4 7.5 5.6 1.9 .4 72 97 18 .8 8.6 6.1 2.5 .0 137 115 13 1.3 8.6 6.4 2.2 .1 88 91 15 .8 8.5 6.0 2.5 .3 150 98 13 .5 8.0 5.7 2.3 .3 97 95 12 .7 8.5 6.2 2.3 .1 92 113 14 .7 7.9 5.5 2.4 .2 106 106 12 .7 7.4 5.3 2.1 .1 91 98 11 .8 8.3 5.9 2.4 .0 106 100 10 .5 8.1 5.7 2.4 .0 142 MEAN S.D. N 102 13 .7 8.1 5.8 2.3 8.4 2.3 .25 .44 .34 .19 10 10 10 10 10 10 .2 108 .14 26.1 10 10 Covance 6329-183 3M T-6295 1635 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 100 12 .8 8.1 5.3 2.8 .1 121 98 16 1.0 8.6 5.3 3.3 .0 130 70 14 .5 8.2 5.1 3.1 .2 100 103 9 .9 8.2 6.2 2.0 .0 89 92 14 .8 8.0 5.7 2.3 .1 91 85 16 .9 7.6 5.1 2.5 .3 96 117 12 .9 7.7 5.2 2.5 .0 77 100 12 .7 7.4 5.3 2.1 .2 118 107 14 1.0 8.8 6.3 2.5 .0 106 90 15 .8 7.6 5.7 1.9 .0 85 MEAN S.D. N 96 13 .8 8.0 5.5 2.5 12.9 2.2 .15 .45 .44 .46 10 10 10 10 10 10 .1 101 .11 17.2 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 91 18 .8 8.1 5.8 2.3 .0 81 98 15 .6 8.4 5.9 2.5 .0 90 106 15 .5 8.7 6.2 2.5 .2 155 96 11 .8 8.2 5.9 2.3 .1 115 94 16 .8 7.6 5.3 2.3 .0 58 98 13 .8 8.3 6.4 1.9 .3 86 96 13 .9 8.2 5.6 2.6 .2 96 85 10 .4 7.8 5.9 1.9 .0 80 105 17 .9 8.2 5.8 2.4 .2 104 102 10 1.0 8.4 5.5 2.9 .3 230 MEAN S.D. N 97 14 .8 8.2 5.8 2.4 6.3 2.9 .19 .31 .32 .30 10 10 10 10 10 10 .1 110 .13 49.6 10 10 Covance 6329-183 3M T-6295 1636 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 85 15 .7 7.5 5.3 2.2 .0 89 100 14 .6 8.4 6.8 1.6 .1 86 107 22 .7 8.9 5.9 3.0 .0 141 84 15 .6 6.6 4.4 2.2 .0 67 104 19 .8 7.7 5.8 1.9 .0 63 103 13 .9 8.5 6.2 2.3 .3 48 92 17 .9 8.7 6.5 2.2 .1 87 86 17 1.0 6.9 4.3 2.6 .0 59 109 13 .6 7.9 5.8 2.1 .0 73 105 17 .7 7.6 5.7 1.9 .0 81 MEAN S.D. N 98 16 .8 7.9 5.7 2.2 .0 79 9.8 2.8 .14 .76 .81 .39 .10 25.5 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1637 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 129 54 135 42 82 42 113 39 88 29 283 133 76 32 103 44 105 52 79 34 0 10.8 7.5 147 5.1 102 0 10.4 6.4 142 5.2 107 0 10.0 6.0 145 5.6 110 0 10.0 4.7 146 4.6 107 0 10.5 4.6 144 4.6 106 0 11.4 6.4 150 6.0 109 0 11.4 6.1 147 5.4 103 1 10.4 5.0 144 5.4 108 0 9.9 5.4 143 4.8 104 0 10.7 6.2 147 4.7 107 MEAN S.D. N 119 61.0 10 50 30.2 10 0 .3 10 10.6 .54 10 5.8 .90 10 146 2.4 10 5.1 .47 10 106 2.6 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 102 45 137 42 98 38 101 38 101 52 123 42 126 39 104 32 128 52 132 53 0 10.8 6.1 144 6.3 104 0 11.2 6.7 149 6.0 105 1 12.2 7.6 156 8.5 113 0 11.4 5.7 145 5.3 101 0 11.0 4.9 144 5.5 107 0 11.1 5.7 148 5.1 103 0 10.9 4.8 145 5.1 104 1 10.3 5.6 148 6.0 111 1 11.2 5.6 145 5.8 107 1 11.0 5.5 146 4.9 105 MEAN S.D. N 115 15.3 10 43 7.1 10 0 .5 10 11.1 .48 10 5.8 .83 10 147 3.6 10 5.8 1.04 10 106 3.7 10 Covance 6329-183 3M T-6295 1638 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 85 35 109 40 148 53 119 66 137 80 158 64 107 39 124 65 115 46 111 52 0 10.4 5.1 145 4.6 106 0 11.0 6.3 151 6.0 109 0 10.7 5.9 145 4.8 107 0 10.9 5.0 149 5.2 108 0 11.1 6.7 148 5.4 108 0 10.6 5.3 147 5.3 108 3 10.8 5.2 148 4.9 103 3 10.9 5.8 145 4.6 104 0 10.9 5.1 145 4.6 103 0 10.4 5.9 149 5.3 110 MEAN S.D. N 121 21.4 10 54 14.5 10 1 1.3 10 10.8 .24 10 5.6 .58 10 147 2.1 10 5.1 .45 10 107 2.5 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 130 26 121 75 153 84 128 55 101 40 78 30 107 35 77 49 84 34 98 36 0 10.8 5.3 146 5.1 111 0 10.9 5.6 147 4.5 106 0 11.6 6.7 149 6.1 107 4 10.8 4.1 145 5.2 105 4 10.9 6.3 147 6.6 108 0 11.1 4.8 145 5.3 109 2 11.1 5.9 150 6.1 111 0 10.5 5.3 146 5.1 111 0 11.1 5.8 148 5.5 109 0 11.2 5.4 145 4.8 101 MEAN S.D. N 108 25.1 10 46 19.5 10 1 1.7 10 11.0 .29 10 5.5 .74 10 147 1.8 10 5.4 .65 10 108 3.2 10 Covance 6329-183 3M T-6295 1639 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 99 42 73 36 88 86 129 63 77 27 79 37 98 49 112 49 80 34 95 36 0 10.5 5.2 145 4.6 108 0 11.1 5.5 148 4.8 105 0 10.9 5.9 151 5.8 109 0 10.0 6.0 145 4.8 112 0 10.9 6.8 148 6.7 112 0 10.5 6.1 144 5.8 106 0 11.2 7.1 153 7.2 112 0 9.8 5.8 146 4.8 113 1 10.5 5.9 145 4.3 109 0 10.2 5.0 144 4.5 110 MEAN S.D. N 93 17.5 10 46 17.3 10 0 .3 10 10.6 .47 10 5.9 .65 10 147 3.1 10 5.3 1.00 10 110 2.7 10 Covance 6329-183 3M T-6295 1640 Appendix 5 Individual Clinical Chemistry Data Females Week 103 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92991 C93003 C93007 C93011 C93022 C93039 C93040 C93047 C93048 139 101 66 247 107 73 145 285 145 211 133 174 202 108 79 180 100 143 MEAN S.D. N 124 41.1 9 169 70.4 9 Covance 6329-183 3M T-6295 1641 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92503 C92505 C92510 C92513 C92514 C92518 C92541 C92542 C92544 C92551 C92569 101 42 60 124 116 220 136 187 123 216 328 207 110 54 187 262 120 62 184 590 220 230 MEAN S.D. N 153 73.5 11 199 151.3 11 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 C92583 C92590 C92597 C92598 C92601 C92602 C92619 C92623 C92626 189 134 239 322 147 195 84 108 241 96 125 255 116 193 137 306 86 153 76 110 MEAN S.D. N 144 60.8 10 187 82.7 10 Covance 6329-183 3M T-6295 1642 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92631 C92633 C92637 C92641 C92642 C92643 C92644 C92649 C92653 C92657 C92659 C92660 C92669 C92674 C92682 C92683 C92690 121 218 147 86 202 360 134 182 123 71 181 423 153 375 89 86 198 186 117 152 75 58 128 132 139 114 81 188 91 383 94 100 123 272 MEAN S.D. N 129 38.4 17 199 120.6 17 Covance 6329-183 3M T-6295 1643 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92691 C92693 C92694 C92695 C92696 C92698 C92699 C92700 C92702 C92704 C92705 C92708 C92716 C92717 C92724 C92726 C92730 C92735 C92736 C92739 C92740 C92742 C92747 C92748 C92750 125 183 86 79 97 64 114 184 102 96 103 81 87 145 98 115 135 144 129 140 153 184 144 264 101 151 268 277 154 237 127 137 154 75 119 215 157 231 67 137 77 86 163 218 49 32 145 117 126 169 MEAN S.D. N 123 42.7 25 150 65.3 25 Covance 6329-183 3M T-6295 1644 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92753 C92757 C92761 C92762 C92774 C92775 C92776 C92780 C92782 C92786 C92788 C92791 C92792 C92800 C92802 C92803 C92804 C92808 C92809 C92814 C92815 C92818 97 116 102 107 163 428 99 87 126 204 96 203 43 77 139 88 134 237 185 212 80 38 201 421 91 146 98 142 33 53 67 35 90 111 150 213 214 410 62 55 213 267 102 76 MEAN S.D. N 118 52.2 22 169 121.9 22 Covance 6329-183 3M T-6295 1645 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 6 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92821 C92823 C92827 C92829 C92833 C92834 C92848 C92851 C92853 C92856 94 88 175 145 173 163 104 69 125 147 200 206 85 42 168 169 155 141 174 196 MEAN S.D. N 145 40.1 10 137 53.9 10 Covance 6329-183 3M T-6295 1646 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92866 C92867 C92868 C92869 C92871 C92873 C92874 C92877 C92881 C92882 C92885 C92890 C92892 C92895 C92896 C92897 C92906 C92907 C92909 C92911 C92912 C92913 C92925 C92927 116 52 151 149 115 101 114 98 162 203 108 102 113 91 100 138 114 181 142 123 218 234 94 80 108 108 97 72 123 101 182 46 102 51 147 405 90 188 120 118 83 44 105 55 222 98 58 118 MEAN S.D. N 124 39.6 24 123 78.6 24 Covance 6329-183 3M T-6295 1647 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92936 C92938 C92946 C92953 C92957 C92960 C92964 C92970 C92973 C92974 C92975 C92983 C92984 C92986 C92988 113 51 117 89 138 399 128 100 81 29 116 116 144 130 104 34 119 67 117 61 140 282 89 30 157 110 180 104 110 111 MEAN S.D. N 124 25.4 15 114 100.1 15 Covance 6329-183 3M T-6295 1648 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93059 C93060 C93062 C93063 C93072 C93077 C93078 C93079 C93080 C93081 C93090 C93098 C93101 C93102 C93107 100 160 142 100 126 45 72 73 128 86 115 91 175 63 101 408 88 203 91 97 83 82 169 158 106 207 100 191 118 71 MEAN S.D. N 114 29.8 15 136 92.5 15 Covance 6329-183 3M T-6295 1649 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93113 C93115 C93124 C93125 C93133 C93135 C93137 C93139 C93140 C93146 C93149 C93151 C93153 C93157 C93158 C93161 C93162 C93164 C93165 C93166 C93168 C93174 C93177 C93178 C93179 122 188 160 124 113 77 114 32 61 80 94 56 38 54 76 104 340 585 117 60 149 55 86 51 127 132 119 160 70 59 89 58 80 79 71 56 59 29 86 54 103 117 68 49 84 48 71 38 77 47 MEAN S.D. N 103 57.2 25 96 109.7 25 Covance 6329-183 3M T-6295 1650 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ CHOL MG/DL -------- TRIG MG/DL -------- Group: 6 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93181 C93182 C93183 C93184 C93185 C93190 C93192 C93197 C93198 C93201 C93202 C93205 C93209 C93211 C93216 C93219 C93220 110 133 122 172 78 55 81 124 129 128 139 56 141 175 118 98 94 306 79 89 77 146 145 148 86 134 101 63 111 148 124 310 118 241 MEAN S.D. N 109 23.3 17 149 76.2 17 Covance 6329-183 3M T-6295 1651 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 11.5 1.013 7.0 34.2 1.007 7.0 48.8 a 1.005 7.0 22.6 1.007 7.0 17.5 1.010 7.0 19.2 1.010 7.0 17.4 1.010 7.5 16.5 1.015 7.0 9.5 1.016 7.0 16.8 1.012 7.0 MEAN S.D. N 21.4 11.72 10 1.010 .0036 10 7.0 .16 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 13.2 1.017 7.0 14.4 1.017 7.0 12.2 1.022 7.0 17.5 1.013 7.0 48.5 a 1.002 7.0 18.3 1.012 7.5 42.6 1.004 6.5 6.4 1.027 7.0 14.5 1.016 7.0 38.5 1.005 7.0 MEAN S.D. N 22.6 14.76 10 1.014 .0080 10 7.0 .24 10 a Sample was overflowing at the time of collection. 1652 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 10.3 1.021 6.5 48.1 a 1.001 7.0 48.4 a 1.000 7.0 20.2 1.010 7.0 48.3 a 1.003 7.0 23.3 1.009 7.0 16.1 1.013 6.5 9.3 1.020 7.0 14.1 1.013 7.5 15.2 1.013 7.5 MEAN S.D. N 25.3 16.35 10 1.010 .0073 10 7.0 .33 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 15.5 1.015 7.0 27.3 1.010 7.5 49.9 a 1.004 7.0 49.5 a 1.002 7.0 24.5 1.008 7.0 48.0 a 1.004 7.0 23.5 1.008 7.5 11.2 1.020 7.0 42.8 1.006 7.0 25.5 1.010 7.5 MEAN S.D. N 31.8 14.52 10 1.009 .0055 10 7.2 .24 10 a Sample was overflowing at the time of collection. 1653 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 29.0 1.006 7.0 24.0 1.009 7.0 6.0 1.029 7.0 26.7 1.010 7.0 17.0 1.016 7.0 28.6 1.009 7.0 39.7 1.006 7.0 19.9 1.013 7.0 10.2 1.017 7.0 24.7 1.009 7.0 MEAN S.D. N 22.6 9.78 10 1.012 .0069 10 7.0 .00 10 Covance 6329-183 3M T-6295 1654 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 10 - + - + - C92506 10 - - - - - C92524 0 - - - - - C92526 10 - - - - - C92529 10 - - - - - C92542 10 - - - - - C92543 10 - - - - - C92546 10 - - - - - C92548 10 - + - - - C92553 30 - + - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 10 - + - - - C92574 30 - + - - - C92575 10 - + - - - C92585 10 - - - - - C92586 0 - - - - - C92587 10 - - - - - C92610 0 - - - - - C92613 30 - + - - - C92615 10 - + - - - C92618 10 - - - - - Covance 6329-183 3M T-6295 1655 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 10 - + - - - C92646 0 - - - - - C92650 0 - - - - - C92652 10 - - - - - C92657 10 - - - - - C92668 10 - - - - - C92676 10 - - - - - C92678 10 - - - - - C92684 10 - + - + - C92686 10 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 10 - + - - - C92715 10 - - - - - C92716 10 - - - - - C92718 0 - - - - - C92725 10 - + - - - C92731 0 - - - - - C92732 10 - - - - - C92734 10 - - - - - C92744 10 - + - - - C92746 30 - - - - - Covance 6329-183 3M T-6295 1656 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 10 - + - - - C92759 10 - + - - - C92779 30 - + - - - C92784 10 - - - - - C92793 10 - - - - - C92798 10 - - - - - C92799 10 - - - - - C92805 10 - + - - - C92807 10 - + - - - C92811 10 - + - - - Covance 6329-183 3M T-6295 1657 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 0 0 1 1 0 3D BJ Q C92506 0 0 1 2 0 0 AJ PQ C92524 0 0 0 1 0 0 AJ Q C92526 0 0 1 2 0 0 BK Q C92529 0 0 1 1 0 4D BJ Q C92542 0 0 1 1 0 4D BK PQ C92543 0 0 1 1 0 4D BJ Q C92546 0 0 0 2 0 4D BK PQ C92548 0 0 0 2 0 3D CJ Q C92553 0 0 0 1 0 4D BJ Q Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 0 0 0 3 0 4D CK Q C92574 0 0 1 1 0 3D CK Q C92575 0 0 1 2 0 4D CJ P C92585 0 0 1 1 0 2D BK PQ C92586 0 0 0 2 0 0 AJ Q C92587 0 0 0 1 0 0 CJ - C92610 0 0 1 1 0 0 BJ Q C92613 0 0 1 3 0 4D CK PQ C92615 0 0 0 2 0 4D CJ PQ C92618 0 0 1 1 0 0 AJ - Covance 6329-183 3M T-6295 1658 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 0 0 1 2 0 0 CK Q C92646 0 0 0 2 0 0 AJ Q C92650 0 0 0 1 0 0 AJ Q C92652 0 0 0 1 0 0 BJ PQ C92657 0 0 0 2 0 1B AJ Q C92668 0 0 0 2 0 2D BJ PQ C92676 0 0 1 1 0 0 BJ Q C92678 0 0 0 3 0 4D CK PQ C92684 0 0 1 1 0 4D CK PQ C92686 0 0 0 1 0 4D CJ - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 0 0 0 2 0 2B4D CK Q C92715 0 0 0 1 0 4D BK PQ C92716 0 0 1 1 0 0 AJ Q C92718 0 0 1 1 0 0 AJ Q C92725 0 0 1 1 0 1D BJ Q C92731 0 0 0 3 0 0 AJ Q C92732 0 0 0 1 0 0 BJ Q C92734 0 0 0 2 0 4D CK PQ C92744 0 0 0 3 0 4D BK PQ C92746 0 0 1 1 0 2D BJ Q Covance 6329-183 3M T-6295 1659 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 0 0 1 2 0 0 AJ PQ C92759 0 0 0 1 0 1D BJ Q C92779 0 0 1 1 0 3D CK Q C92784 0 0 0 1 0 1D BJ PQ C92793 0 0 0 2 0 4D CK PQ C92798 0 0 0 3 0 0 BJ Q C92799 0 0 0 3 0 0 BJO Q C92805 0 0 0 1 0 0 BJ PQ C92807 0 0 1 2 0 4D CJ PQ C92811 0 0 0 1 0 0 BJ Q Covance 6329-183 3M T-6295 1660 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 9.0 1.016 7.0 3.4 1.023 7.0 2.2 1.036 6.5 6.6 1.018 6.5 6.4 1.018 6.5 22.5 1.008 7.0 2.7 1.026 7.0 45.2 a 1.001 7.0 9.5 1.015 6.5 1.9 1.030 6.5 MEAN S.D. N 10.9 13.48 10 1.019 .0103 10 6.8 .26 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 1.5 1.036 6.5 47.5 a 1.000 7.0 15.5 1.007 7.0 6.7 1.020 7.0 10.0 1.008 6.5 7.2 1.019 7.0 9.6 1.016 7.0 3.5 1.022 7.0 42.3 a 1.003 7.0 8.5 1.017 6.5 MEAN S.D. N 15.2 16.13 10 1.015 .0106 10 6.8 .24 10 a Sample was overflowing at the time of collection. 1661 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 4.0 1.022 6.5 46.0 a 1.001 6.5 5.0 1.012 7.0 6.2 1.021 6.5 5.9 1.014 7.0 7.7 1.016 7.0 49.3 a 1.000 7.0 4.1 1.023 7.0 6.4 1.021 7.0 7.5 1.023 6.5 MEAN S.D. N 14.2 17.68 10 1.015 .0087 10 6.8 .26 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 6.5 1.017 7.0 14.1 1.010 7.0 5.2 1.021 6.5 8.0 1.023 7.0 9.0 1.016 7.0 18.2 1.008 6.5 12.5 1.008 7.0 11.2 1.018 7.0 29.0 1.006 7.0 18.0 1.011 6.5 MEAN S.D. N 13.2 7.12 10 1.014 .0060 10 6.8 .24 10 a Sample was overflowing at the time of collection. 1662 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 28.7 1.005 7.0 9.5 1.014 7.0 9.8 1.015 7.0 10.0 1.012 6.5 5.1 1.019 6.5 4.1 1.026 6.5 43.0 a 1.002 7.0 48.4 a 1.001 7.0 10.2 1.014 7.0 6.5 1.022 6.5 MEAN S.D. N 17.5 16.39 10 1.013 .0083 10 6.8 .26 10 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1663 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 0 - - - - - C92864 10 - - - - - C92876 10 - - - - - C92877 0 - - - - - C92882 10 - - - - - C92883 0 - - - - - C92914 10 - - - - - C92919 0 - - - - - C92925 0 - - - - - C92926 10 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 30 - - - - - C92945 0 - - - - - C92950 0 - - - - - C92953 10 - - - - - C92959 0 - - - - - C92964 10 - - - - - C92968 10 - - - - - C92969 10 - - - - - C92976 0 - - - - - C92982 0 - - - - - Covance 6329-183 3M T-6295 1664 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 10 - - - - - C92996 0 - - - - - C92998 0 - - - - - C93004 0 - - - - - C93006 10 - - - - - C93008 0 - - - - - C93023 0 - - - - - C93031 10 - - - - - C93033 10 - - - - - C93045 0 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 10 - - - - - C93067 0 - - - - - C93070 10 - - - - - C93074 10 - - - - - C93075 0 - - - - - C93083 0 - - - - - C93084 0 - - - - - C93089 0 - - - - - C93094 0 - - - - - C93105 0 - - - - - Covance 6329-183 3M T-6295 1665 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 0 - - - - - C93123 0 - - - - - C93142 0 - - - - - C93144 0 - - - - - C93151 10 - - - - - C93155 0 - - - - - C93158 0 - - - - - C93159 0 - - - - - C93164 0 - - - - - C93175 0 - - - - - Covance 6329-183 3M T-6295 1666 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 0 0 0 1 0 2D CJ - C92864 0 0 1 1 0 2D CJ - C92876 0 0 1 1 0 2D CK P C92877 0 1 1 1 0 1B1D CJ P C92882 0 0 1 1 0 1D CJ - C92883 0 0 1 2 0 0 AJ - C92914 0 0 1 2 0 4D CJ P C92919 0 0 0 1 0 0 AJ - C92925 0 0 1 1 0 2B CJ - C92926 0 1 0 1 0 0 CJ P Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 0 0 1 1 0 0 CJ P C92945 0 0 0 1 0 0 AJ - C92950 0 1 1 2 0 0 BJ P C92953 0 0 1 1 0 3D CK - C92959 0 1 0 1 0 0 BJ - C92964 0 1 0 1 0 2D CJ - C92968 0 0 1 2 0 4D CJ P C92969 0 1 0 1 0 3D CJ P C92976 0 1 1 1 0 0 AJ - C92982 0 0 0 1 0 2D CK - Covance 6329-183 3M T-6295 1667 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 0 1 1 1 0 1D CJ P C92996 0 0 1 1 0 0 AJ - C92998 0 1 1 1 0 0 BJ P C93004 0 0 1 1 0 1D CJ - C93006 0 0 0 1 0 3D BJ - C93008 0 0 0 1 0 2D CJ - C93023 0 0 0 1 0 0 AJ - C93031 0 0 0 1 0 4D CJ P C93033 0 0 1 1 0 4D CK - C93045 0 0 1 1 0 4D CK P Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 0 0 0 1 0 4D CK - C93067 0 0 0 1 0 3D BJ - C93070 0 1 1 2 0 0 CK P C93074 0 1 1 1 0 4D CK P C93075 0 1 1 1 0 4D CJ P C93083 0 1 0 1 0 1B BJ P C93084 0 1 1 1 0 0 BJ P C93089 0 1 0 1 1G 2D CJ P C93094 0 1 1 2 0 0 AJ - C93105 0 1 1 2 0 1D BJ P Covance 6329-183 3M T-6295 1668 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 0 0 0 1 0 0 BJ - C93123 0 0 1 1 0 4D BJ - C93142 0 0 1 1 0 4D BJ - C93144 0 0 1 2 0 0 BJ P C93151 0 0 1 1 0 2D CJ P C93155 0 0 1 1 0 2D CJ P C93158 0 0 0 1 0 1B AJ - C93159 0 0 0 1 0 0 AJ - C93164 0 1 1 1 0 1D BJ - C93175 0 1 1 1 0 2D CJ - Covance 6329-183 3M T-6295 1669 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 30.2 1.010 7.5 19.1 1.012 7.5 31.4 1.009 7.0 9.1 1.027 7.0 26.0 1.015 7.5 49.5 a 1.003 7.0 20.0 1.015 7.0 4.5 1.035 6.5 49.5 a 1.006 7.0 49.1 a 1.008 7.5 MEAN S.D. N 28.8 16.45 10 1.014 .0099 10 7.2 .34 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 16.8 1.016 7.5 46.3 a 1.003 6.5 23.5 1.014 7.0 7.4 1.022 7.0 49.3 a 1.006 7.5 28.7 1.008 7.5 3.4 1.044 6.5 17.0 1.018 7.5 35.6 1.008 7.0 7.3 1.040 7.0 MEAN S.D. N 23.5 16.23 10 1.018 .0140 10 7.1 .39 10 a Sample was overflowing at the time of collection. 1670 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 46.5 a 1.004 6.5 6.8 1.025 6.5 9.4 1.026 7.0 9.7 1.022 7.0 14.8 1.021 7.5 40.5 1.007 7.5 10.1 1.020 7.0 2.0 1.032 6.5 20.5 1.016 7.0 47.5 a 1.003 7.0 MEAN S.D. N 20.8 17.37 10 1.018 .0099 10 7.0 .37 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 10.1 1.021 7.0 47.7 a 1.005 7.0 22.1 1.013 7.0 26.3 1.013 7.5 12.0 1.021 6.5 35.0 1.005 6.5 12.8 1.021 7.0 15.5 1.020 7.0 49.0 a 1.007 7.5 23.5 1.016 7.0 MEAN S.D. N 25.4 14.27 10 1.014 .0067 10 7.0 .33 10 a Sample was overflowing at the time of collection. 1671 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 9.3 1.020 7.0 9.8 1.024 7.0 37.0 1.010 7.0 12.5 1.020 7.0 48.1 1.007 7.0 12.0 1.021 7.0 5.5 1.028 6.5 12.7 1.025 6.5 7.6 1.027 7.0 30.8 1.012 7.5 MEAN S.D. N 18.5 14.64 10 1.019 .0073 10 7.0 .28 10 Covance 6329-183 3M T-6295 1672 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 10 - - - - - C92509 10 - - - - - C92511 10 - - - + - C92521 30 - + - - - C92527 30 - + - - - C92528 0 - - - - - C92532 10 - - - - - C92535 30 - + - - - C92537 0 - - - - - C92562 10 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 30 - - - - - C92593 0 - - - - - C92598 10 - - - - - C92600 30 - - - - - C92612 0 - - - - - C92616 10 - - - - - C92621 30 - + - + - C92624 30 - + - - - C92627 10 - - - - - C92629 30 - + - - - Covance 6329-183 3M T-6295 1673 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 0 - - - - - C92640 30 - - - - - C92644 10 - + - - - C92645 30 - + - - - C92651 30 - - - - - C92661 10 - - - - - C92662 30 - + - - - C92676 30 - + - - - C92682 10 - - - - - C92684 0 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 30 - + - - - C92713 10 - - - - - C92714 10 - + - - - C92716 10 - + - - - C92719 10 - - - - - C92722 10 - - - - - C92726 30 - + - - - C92728 30 - + - - - C92741 10 - - - - - C92748 10 - - - - - Covance 6329-183 3M T-6295 1674 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 10 - + - - - C92765 30 - - - - - C92767 10 - - - - - C92774 10 - + - - - C92777 10 - - - - - C92784 30 - + - - - C92789 10 - + - + - C92799 30 - - - + - C92812 30 - - - - - C92820 10 - + - - - Covance 6329-183 3M T-6295 1675 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 0 0 1 1 0 4D BJ Q C92509 0 0 1 1 0 4D BJ Q C92511 0 1 1 1 0 1B1D BJ PQ C92521 0 0 0 2 0 4D BK P C92527 0 0 1 2 0 4D BK Q C92528 0 0 0 1 0 0 AJ Q C92532 0 0 1 1 0 4D BK Q C92535 0 0 1 1 0 3D CK Q C92537 0 0 1 2 0 2D AJ Q C92562 0 0 1 1 0 2D AJ Q Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 0 0 1 2 0 4D BK Q C92593 1 0 1 1 0 0 AJ Q C92598 0 0 1 1 0 4D BK Q C92600 1 0 0 1 0 4D BK PQ C92612 0 0 1 1 0 3D AJ Q C92616 0 0 0 1 0 3D BK Q C92621 0 1 1 1 0 4D DK PQ C92624 0 1 1 1 0 4D BK Q C92627 0 0 1 1 0 3D BJ Q C92629 0 0 1 2 0 3D CK PQ Covance 6329-183 3M T-6295 1676 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 0 0 1 2 0 0 AJ Q C92640 0 0 0 2 0 4D CK Q C92644 0 0 1 2 0 4D CK Q C92645 0 0 1 2 0 4D BK P C92651 0 0 0 1 0 4D CK Q C92661 0 0 1 2 0 4D AJ Q C92662 0 1 0 1 0 3D BK PQ C92676 0 0 1 1 0 0 BJ Q C92682 0 0 1 1 0 4D BK PQ C92684 0 0 1 1 0 0 AJ Q Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 0 0 1 1 0 4D CK PQ C92713 0 0 1 1 0 0 AJ Q C92714 0 0 1 1 0 1D BJ Q C92716 0 0 0 1 0 4D BK Q C92719 0 1 1 1 0 2D BK PQ C92722 0 0 1 3 0 0 AJ Q C92726 0 0 1 1 0 3D BK Q C92728 0 2 0 2 0 4D BK Q C92741 0 0 1 1 0 3D AJ Q C92748 0 0 1 1 0 4D BK Q Covance 6329-183 3M T-6295 1677 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 0 0 1 1 0 4D CK Q C92765 0 0 0 1 0 3D BK Q C92767 0 0 1 2 0 0 AJ Q C92774 0 0 0 1 0 4D CJ PQ C92777 0 0 1 1 0 0 BJ Q C92784 0 0 1 2 0 1B4D CJ PQ C92789 2 1 1 1 0 1D BK PQ C92799 0 0 1 1 0 1D BJ Q C92812 0 0 1 2 0 4D BK PQ C92820 0 0 1 2 0 2B4D BJ PQ Covance 6329-183 3M T-6295 1678 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 4.3 1.033 7.0 7.0 1.023 7.0 .7 1.056 7.0 2.3 1.025 6.5 6.2 1.020 6.5 4.5 1.022 6.5 6.5 1.020 7.0 49.5 a 1.002 6.5 48.5 a 1.003 7.0 45.5 a 1.001 6.5 MEAN S.D. N 17.5 21.04 10 1.020 .0166 10 6.8 .26 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 3.2 1.030 7.0 18.5 1.006 8.0 49.9 a 1.003 7.0 7.0 1.020 6.5 12.3 1.015 7.5 49.2 a 1.003 6.5 6.9 1.021 6.5 11.5 1.019 6.5 6.5 1.018 7.0 3.1 1.022 7.0 MEAN S.D. N 16.8 17.86 10 1.016 .0090 10 7.0 .50 10 a Sample was overflowing at the time of collection. 1679 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 18.8 1.008 7.0 3.6 1.024 6.5 5.0 1.019 6.5 8.0 1.015 7.0 20.4 1.010 7.5 4.2 1.026 6.5 5.0 1.019 7.0 3.4 1.029 7.0 2.7 1.033 6.5 7.1 1.027 6.5 MEAN S.D. N 7.8 6.43 10 1.021 .0082 10 6.8 .35 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 8.7 1.019 6.5 16.0 1.008 7.0 48.0 a 1.002 6.5 19.6 1.009 7.0 45.5 a 1.003 7.0 12.1 1.012 6.5 21.5 1.012 7.0 41.5 1.006 7.5 25.8 1.013 6.5 18.5 1.010 6.5 MEAN S.D. N 25.7 14.20 10 1.009 .0050 10 6.8 .35 10 a Sample was overflowing at the time of collection. 1680 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 13.5 1.014 7.0 12.2 1.010 7.0 8.0 1.018 7.5 38.0 1.003 6.5 46.5 a 1.004 7.0 6.0 1.022 7.0 1.5 1.043 6.5 13.2 1.012 7.0 7.2 1.020 6.5 49.0 a 1.005 7.0 MEAN S.D. N 19.5 17.83 10 1.015 .0119 10 6.9 .32 10 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1681 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 30 - - - - - C92865 10 - - - - - C92871 30 - - - - - C92880 10 - - - - - C92887 10 - - - - - C92898 10 - - - - - C92903 10 - - - - - C92905 0 - - - - - C92906 0 - - - - - C92927 0 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 10 - - - - - C92940 0 - - - + - C92944 0 - - - - - C92948 10 - - - - - C92956 10 - - - - - C92962 0 - - - - - C92967 10 - - - - - C92978 30 - - - - - C92986 10 - - - - - C92987 10 - - - - - Covance 6329-183 3M T-6295 1682 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 0 - - - - - C92993 10 - - - - - C93000 10 - - - - - C93018 10 - - - - - C93019 0 - - - - - C93023 10 - - - - - C93024 10 - - - - - C93026 30 - - - - - C93030 30 - - - - - C93035 10 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 10 - - - - - C93064 0 - - - - - C93071 0 - - - - - C93076 0 - - - - - C93078 0 - - - - - C93085 0 - - - - - C93089 0 - - - - - C93090 0 - - - - - C93093 0 - - - - - C93109 0 - - - - - Covance 6329-183 3M T-6295 1683 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 0 - - - - - C93113 0 - - - - - C93120 10 - - - - - C93127 0 - - - - - C93135 0 - - - - - C93143 10 - - - - - C93155 10 - - - - - C93162 0 - - - - - C93169 10 - - - - - C93178 0 - - - - - Covance 6329-183 3M T-6295 1684 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 1 0 0 1 0 0 CK - C92865 0 0 1 1 0 0 CJ S C92871 QS - - - - - - DJ - C92880 0 0 0 2 0 0 BJ P C92887 0 0 1 1 0 0 BJ - C92898 0 0 1 1 0 1D BJ P C92903 0 0 1 2 0 2D BJ - C92905 0 0 1 1 0 1D AJ - C92906 0 0 0 1 0 0 AJ - C92927 0 0 1 1 0 0 AJ - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 0 1 1 2 0 4D CJ P C92940 0 0 0 3 0 3B4D CJ - C92944 0 0 0 1 0 0 AJ - C92948 0 0 0 1 0 1B1D CJ P C92956 0 0 1 2 0 2D CJ P C92962 0 0 0 1 0 0 AJ - C92967 0 0 1 1 0 1D BJ P C92978 0 0 1 1 0 1D BJ P C92986 0 0 1 2 0 4D CJ P C92987 0 0 0 1 0 4D BJ - Covance 6329-183 3M T-6295 1685 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 0 0 1 1 0 0 AJ - C92993 0 0 1 3 0 4B BJ - C93000 0 0 1 1 0 3D BJ P C93018 0 0 1 2 0 4D BJ P C93019 0 1 1 1 0 1B1D BJ - C93023 0 0 1 1 0 1D CJ P C93024 0 0 1 1 0 1D CJ - C93026 0 1 1 2 0 2D CK - C93030 0 0 1 1 0 2D CJ P C93035 0 1 1 1 0 4D BJ P Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 0 0 1 1 0 1D BJ P C93064 0 0 0 1 0 1B BJ P C93071 0 1 1 1 0 0 AJ - C93076 0 0 1 1 0 0 AJ P C93078 0 0 1 1 0 0 AJ - C93085 0 0 1 1 0 0 BJ - C93089 0 0 0 1 0 4D BJ - C93090 0 1 1 1 0 1D AJ - C93093 0 0 0 1 0 1D BK - C93109 0 0 0 1 0 0 BJ - Covance 6329-183 3M T-6295 1686 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 0 0 1 1 0 4D BJ P C93113 0 0 0 1 0 0 BJ P C93120 0 0 0 1 0 4D CJ - C93127 0 1 0 1 0 0 AJ - C93135 0 0 0 3 0 0 AJ - C93143 0 0 1 1 0 4D BJ P C93155 0 0 1 1 0 4D CJ P C93162 0 1 1 2 0 2B BJ P C93169 0 0 1 1 0 0 BJ - C93178 0 0 1 1 0 0 AJ - Covance 6329-183 3M T-6295 1687 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 4.5 1.028 6.5 4.6 1.047 6.5 16.2 1.017 6.5 19.0 1.017 7.5 15.4 1.022 7.0 18.4 1.016 7.0 22.2 1.014 7.0 7.5 1.031 7.0 23.5 1.014 7.0 20.4 1.016 7.0 MEAN S.D. N 15.2 7.12 10 1.022 .0105 10 6.9 .32 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 12.2 1.019 7.0 10.6 1.018 7.0 10.6 1.024 7.0 13.0 1.020 7.0 14.6 1.016 7.5 28.2 1.010 7.0 10.0 1.021 7.0 17.8 1.012 7.5 11.0 1.025 7.0 34.5 1.009 7.0 MEAN S.D. N 16.2 8.42 10 1.017 .0056 10 7.1 .21 10 Covance 6329-183 3M T-6295 1688 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 17.1 1.021 7.0 10.6 1.026 7.0 30.5 1.010 6.5 11.5 1.022 7.5 9.2 1.024 7.0 21.9 1.013 7.5 10.4 1.024 6.5 17.4 1.015 7.0 12.0 1.019 7.0 20.9 1.017 6.5 MEAN S.D. N 16.2 6.79 10 1.019 .0053 10 7.0 .37 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 16.1 1.017 7.0 47.7 a 1.004 6.5 11.2 1.024 7.0 23.0 1.015 7.0 8.6 1.030 7.0 14.5 1.013 7.5 20.2 1.015 7.0 8.1 1.039 7.0 5.6 1.024 7.0 49.0 a 1.006 7.0 MEAN S.D. N 20.4 15.70 10 1.019 .0107 10 7.0 .24 10 a Sample was overflowing at the time of collection. 1689 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 16.5 1.022 7.0 7.0 1.027 6.5 1.5 1.086 6.5 16.1 1.019 7.0 8.8 1.038 6.5 47.7 a 1.007 7.0 10.4 1.030 7.0 19.5 1.014 7.0 4.9 1.037 7.0 46.8 a 1.006 6.5 MEAN S.D. N 17.9 16.42 10 1.029 .0231 10 6.8 .26 10 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1690 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 10 - + - - - C92510 30 - + - +++ - C92512 30 - - - - - C92525 30 - + - +++ - C92537 10 - - - - - C92543 10 - - - - - C92545 10 - + - - - C92563 300 - - - - - C92567 10 - - - - - C92570 100 - + - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 10 - - - - - C92583 10 - + - - - C92585 100 - - - - - C92586 10 - - - - - C92588 10 - - - - - C92590 10 - - - - - C92601 10 - + - - - C92602 10 - - - - - C92617 30 - - - - - C92630 100 - - - - - Covance 6329-183 3M T-6295 1691 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 10 - + - - - C92644 10 - - - - - C92647 0 - - - - - C92658 30 - - - - - C92660 30 - + - - - C92667 10 - - - + - C92673 10 - - - - - C92679 10 - + - - - C92686 10 - - - - - C92687 100 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 10 - + - - - C92698 0 - - - - - C92708 10 - ++ - - - C92709 300 - - - - - C92710 30 - + - - - C92725 10 - + - - - C92743 10 - + - - - C92745 100 - + - - - C92749 10 - + - - - C92750 0 - - - - - Covance 6329-183 3M T-6295 1692 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 30 - + - - - C92761 10 - - - - - C92764 300 - + - - - C92773 10 - - - - - C92783 100 - + - - - C92788 0 - - - - - C92791 30 - + - - - C92803 10 - - - - - C92804 30 - + - - - C92820 10 - - - - - Covance 6329-183 3M T-6295 1693 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 0 1 1 2 0 3D BJ PQ C92510 4 0 1 1 0 3D CK Q C92512 0 0 1 2 0 0 BJ PQ C92525 1 0 1 2 0 3D BJ PQ C92537 0 1 0 1 0 4D BJ Q C92543 0 0 0 1 0 4D BJ PQ C92545 0 0 1 2 0 1B3D BJ Q C92563 0 1 1 2 1G 1B1D BJ PQ C92567 0 0 1 3 0 1D1E BJ Q C92570 0 0 1 2 0 1D BJ Q Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 0 0 1 2 0 4D BJ PQ C92583 0 1 1 2 0 4D BJ PQ C92585 0 1 0 2 0 3D BJ PQ C92586 0 0 1 2 0 2B2D BJ PQ C92588 0 0 1 3 0 4D BJ PQ C92590 0 1 1 1 0 1B1D AJ Q C92601 0 1 1 2 0 2B2D BJ PQ C92602 0 1 1 2 0 4D BJ PQ C92617 0 0 1 1 0 2D BJ Q C92630 0 1 1 1 0 1D AJ Q Covance 6329-183 3M T-6295 1694 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 0 1 1 2 0 2B2D BJ PQ C92644 0 0 1 3 0 4D BJ PQ C92647 0 1 1 2 0 1B1D AJ Q C92658 0 0 1 2 0 3D BJ Q C92660 0 1 1 2 0 4D BJ Q C92667 0 0 0 2 0 2B2D BJ Q C92673 0 0 0 1 0 1D BJ Q C92679 0 0 1 1 0 1B1D BJ Q C92686 0 1 1 2 0 1B1D BJ Q C92687 0 1 1 2 1H 1B1D BJ Q Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 0 0 1 2 0 4D BJ Q C92698 0 0 1 1 0 1D AJ Q C92708 0 0 1 1 0 3D BJ Q C92709 0 0 1 2 0 4D BJ Q C92710 0 0 1 3 0 4D BJ Q C92725 0 0 1 2 0 4D BJ PQ C92743 0 1 1 2 0 1B1D BJ Q C92745 0 1 1 2 0 4D BJ PQ C92749 0 0 0 2 0 4D BJ PQ C92750 0 0 1 1 0 1D AJ Q Covance 6329-183 3M T-6295 1695 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 0 0 1 3 0 3D BJ PQ C92761 1 0 1 2 0 0 BJ Q C92764 0 0 0 1 0 4D CK PQ C92773 0 0 0 2 0 4D BJ Q C92783 0 0 1 2 0 3D BJ PQ C92788 0 0 1 1 0 1B AJ Q C92791 0 0 0 2 0 2D BJ PQ C92803 0 0 1 1 0 2D BJ PQ C92804 0 1 1 2 0 2D BJ Q C92820 0 1 1 1 0 1D AJ Q Covance 6329-183 3M T-6295 1696 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 1.8 1.046 6.5 10.5 1.014 6.5 7.0 1.022 6.5 26.4 1.004 6.5 13.6 1.018 7.0 5.2 1.031 6.5 47.4 a 1.002 6.5 47.0 a 1.002 6.5 13.1 1.012 6.5 4.0 1.006 7.0 MEAN S.D. N 17.6 17.05 10 1.016 .0142 10 6.6 .21 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 13.6 1.018 6.5 6.1 1.026 7.0 20.4 1.007 6.5 8.0 1.017 7.0 22.5 1.006 6.5 11.5 1.018 6.5 14.8 1.016 7.0 47.0 a 1.002 6.5 9.5 1.016 7.0 5.2 1.031 6.0 MEAN S.D. N 15.9 12.34 10 1.016 .0089 10 6.6 .34 10 a Sample was overflowing at the time of collection. 1697 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 42.2 1.006 7.0 3.4 1.024 7.0 21.7 1.009 7.0 9.1 1.019 7.0 12.8 1.012 7.0 4.0 1.034 7.0 6.9 1.019 6.5 19.5 1.008 6.5 29.7 1.006 6.5 6.2 1.021 7.0 MEAN S.D. N 15.6 12.72 10 1.016 .0092 10 6.8 .24 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 46.0 a 1.002 6.5 5.5 1.032 6.5 9.5 1.018 7.0 30.5 1.006 7.0 39.2 1.006 7.0 19.2 1.010 7.5 24.9 1.010 7.0 1.7 1.032 7.0 20.0 1.012 7.0 19.5 1.013 6.5 MEAN S.D. N 21.6 14.18 10 1.014 .0104 10 6.9 .32 10 a Sample was overflowing at the time of collection. 1698 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 24.4 1.005 6.5 5.8 1.025 6.5 1.2 1.062 6.5 13.7 1.011 6.5 11.5 1.013 7.0 11.2 1.017 7.0 40.9 1.006 6.0 9.0 1.022 7.0 15.8 1.018 6.0 3.9 1.036 6.5 MEAN S.D. N 13.7 11.58 10 1.022 .0170 10 6.6 .37 10 Covance 6329-183 3M T-6295 1699 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 100 - - - + - C92878 0 - - - + - C92888 0 - - - - - C92906 0 - - - + - C92916 100 - - - - - C92917 10 - - - - - C92921 0 - - - - - C92922 0 - - - - - C92923 0 - - - - - C92928 0 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 0 - - - - - C92935 10 - - - - - C92938 0 - - - - - C92951 10 - - - - - C92954 0 - - - - - C92956 0 - - - - - C92960 0 - - - - - C92974 0 - - - - - C92979 10 - - - - - C92989 0 - - - - - Covance 6329-183 3M T-6295 1700 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 0 - - - - - C93004 10 - - - - - C93011 0 - - - - - C93020 0 - - - - - C93027 0 - - - - - C93030 10 - - - - - C93034 0 - - - - - C93046 0 - - - - - C93047 0 - - - - - C93049 0 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 0 - - - - - C93055 0 - - - - - C93072 10 - - - - - C93080 0 - - - - - C93081 0 - - - - - C93090 0 - - - - - C93094 0 - - - - - C93095 10 - - - - - C93103 0 - - - - - C93104 0 - - - - - Covance 6329-183 3M T-6295 1701 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 0 - - - - - C93131 10 - - - - - C93133 30 - - - - - C93138 0 - - - - - C93149 0 - - - - - C93153 0 - - - - - C93157 0 - - - - - C93160 10 - - - - - C93162 0 - - - - - C93179 10 - - - - - Covance 6329-183 3M T-6295 1702 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 0 0 1 1 0 0 CJ P C92878 0 0 0 1 0 0 BJ P C92888 0 0 0 1 0 1B BJ P C92906 0 0 0 1 0 0 AJ - C92916 0 0 0 2 0 1D BJ P C92917 0 0 0 1 0 0 BJ P C92921 0 0 1 3 0 0 AJ - C92922 0 0 1 1 0 0 AJ - C92923 0 0 1 1 0 0 BJ P C92928 0 0 0 1 0 0 AJ - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 0 1 1 1 0 0 BJ P C92935 0 0 1 1 0 3D BJ P C92938 0 0 1 1 0 0 AJ P C92951 0 0 1 2 0 2B BJ P C92954 0 0 1 1 0 0 AJ P C92956 0 1 1 3 0 0 BJ - C92960 0 0 1 1 0 0 BJ - C92974 0 0 0 2 0 0 AJ - C92979 0 0 0 2 0 2D BJ P C92989 0 1 1 1 0 0 BJ P Covance 6329-183 3M T-6295 1703 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 0 0 1 1 0 2D AJ - C93004 0 0 1 1 0 0 BJ - C93011 0 0 1 1 0 3D AJ P C93020 0 0 0 1 0 0 BJ - C93027 0 1 1 1 0 0 AJ P C93030 0 0 1 3 0 4D BJ P C93034 0 1 0 2 0 2B BJ P C93046 0 0 1 2 0 0 AJ P C93047 0 0 1 2 0 0 AJ - C93049 0 0 1 1 0 4D BJ P Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 0 0 1 1 0 0 AJ - C93055 0 0 1 2 0 2B BJ - C93072 0 0 1 2 0 1B BJ PT C93080 0 0 1 1 0 0 AJ - C93081 0 0 1 1 0 0 AJ - C93090 0 0 1 2 0 3D AJ P C93094 0 0 0 1 0 3D AJ P C93095 QS - - - - - - BJ - C93103 0 0 1 2 0 3D BJ P C93104 0 0 1 2 0 1B BJ P Covance 6329-183 3M T-6295 1704 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 0 0 1 1 0 0 AJ - C93131 0 0 0 2 0 1B BJ P C93133 0 0 1 1 0 4D CJ P C93138 0 0 1 1 0 0 AJ P C93149 0 0 1 2 0 0 BJ P C93153 0 0 1 1 0 0 BJ P C93157 0 0 0 1 0 0 AJ - C93160 0 0 1 1 0 0 BJ P C93162 0 0 1 2 0 2B BJ - C93179 0 0 1 1 0 0 BJ P Covance 6329-183 3M T-6295 1705 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 13.3 1.030 7.0 19.0 1.014 6.5 30.5 1.007 7.0 9.6 1.021 6.5 6.5 1.043 6.5 6.8 1.028 6.5 4.6 1.038 6.0 9.0 1.029 6.5 9.5 1.023 6.5 16.2 1.016 7.0 MEAN S.D. N 12.5 7.75 10 1.025 .0110 10 6.6 .32 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 10.2 1.034 6.5 19.0 1.024 7.0 9.6 1.030 6.5 15.8 1.023 7.0 28.0 1.015 7.0 16.0 1.020 7.0 2.1 1.050 6.5 48.0 a 1.011 6.5 6.7 1.041 6.5 24.8 1.017 6.5 MEAN S.D. N 18.0 13.20 10 1.026 .0123 10 6.7 .26 10 a Sample was overflowing at the time of collection. 1706 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 8.7 1.026 6.5 11.2 1.021 6.5 10.1 1.019 7.0 16.2 1.013 7.0 24.5 1.018 7.5 30.0 1.014 7.5 26.5 1.015 7.0 14.5 1.023 7.5 10.2 1.030 7.5 10.0 1.030 CO MEAN S.D. N 16.2 7.90 10 1.021 .0063 10 7.1 .42 9 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 28.5 1.011 6.5 4.0 1.051 7.0 8.5 1.025 7.0 9.0 1.028 7.5 12.6 1.025 6.5 11.0 1.025 7.0 8.2 1.034 6.5 11.4 1.021 7.5 3.2 1.074 6.5 19.8 1.021 7.0 MEAN S.D. N 11.6 7.53 10 1.032 .0182 10 6.9 .39 10 Covance 6329-183 3M T-6295 1707 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 9.4 1.032 7.0 11.0 1.023 7.5 3.0 1.038 6.0 12.2 1.026 7.0 6.7 1.037 7.0 17.4 1.020 6.5 16.0 1.020 6.5 9.2 1.033 7.0 14.2 1.020 7.5 MEAN S.D. N 11.0 4.55 9 1.028 .0074 9 6.9 .49 9 Covance 6329-183 3M T-6295 1708 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 300 - - - - - C92504 100 - - - - - C92507 100 - - - - - C92512 100 - - - - - C92517 300 - - - - - C92523 100 - - - +++ - C92534 100 - - - - - C92540 300 - - - - - C92562 30 - - - - - C92570 300 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 300 - - - - - C92587 300 - - - - - C92592 100 - - - - - C92596 100 - - - - - C92604 30 - - - - - C92612 30 - - - - - C92614 300 - - - - - C92624 300 - - - - - C92625 300 - - - - - C92629 300 - - - - - Covance 6329-183 3M T-6295 1709 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 100 - C92635 30 - C92643 30 - C92649 10 - C92653 100 - C92654 30 - C92667 100 - C92669 30 - C92680 300 - C92686 CO - - - - - - - - - - - - - - - - - - - - - - - - - - - +++ - - - - - - - - - CO CO +++ CO Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 10 - - - - - C92704 100 - - - ++ - C92710 30 - - - - - C92711 100 - - - - - C92727 300 - - - - - C92729 300 - - - - - C92739 30 - - - - - C92741 30 - - - - - C92742 300 - - - - - C92748 300 - - - - - Covance 6329-183 3M T-6295 1710 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 30 - - - - - C92754 30 - + - - - C92755 100 - - - + - C92778 100 - - - - - C92790 300 - + - - - C92795 30 - - - - - C92796 30 - - - - - C92817 30 - - - - - C92820 30 - - - - - Covance 6329-183 3M T-6295 1711 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 0 0 1 3 1G 1D BJ P C92504 1 1 1 1 0 0 BJ P C92507 0 0 0 2 0 0 AJ P C92512 0 0 1 1 0 0 BJ - C92517 0 0 1 3 0 4D CJ PQ C92523 0 0 1 2 0 4D BJ PQ C92534 0 0 1 1 0 0 BJ PQ C92540 0 1 1 3 1G 4D BJ PQ C92562 0 0 1 1 0 4D BJ P C92570 0 1 1 1 0 1B BJ P Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 0 0 0 2 1G 0 CK P C92587 0 0 0 1 0 0 CK - C92592 0 0 1 3 0 4D CJ PQ C92596 0 1 1 2 0 1B CK P C92604 0 1 0 1 0 1B1D BJ P C92612 0 0 0 2 0 2D CK Q C92614 0 0 0 2 0 4D CJ PQ C92624 0 0 0 2 1G1H 0 AJ P C92625 0 1 1 2 1G 1B1D CK PQ C92629 0 1 1 2 1G1I 2B BK PQ Covance 6329-183 3M T-6295 1712 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 0 0 1 1 0 0 CK PQ C92635 0 1 0 1 0 1B CK PQ C92643 0 0 1 1 0 4D CK PQ C92649 0 0 1 2 0 0 BJ PQ C92653 0 0 0 2 0 4D BK Q C92654 0 0 1 2 0 4D BK PQ C92667 1 0 0 2 0 0 BK Q C92669 0 0 1 1 0 4D CK PQ C92680 0 0 1 2 0 4D CK PQ C92686 4 0 0 0 0 0 GL - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 0 0 0 1 0 0 BJ PQ C92704 1 0 1 1 0 2D DK PQ C92710 0 0 0 1 0 1B CK PQ C92711 0 0 1 2 0 4D CK PQ C92727 0 0 1 1 0 0 CK PQ C92729 0 0 1 2 0 2B4D BJ PQ C92739 0 1 1 2 0 1B1D CK PS C92741 0 0 1 2 0 4D BJ P C92742 0 0 1 1 0 3D DK Q C92748 0 0 1 1 0 2D CK PQ Covance 6329-183 3M T-6295 1713 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 0 0 1 3 0 4D CJ PQ C92754 0 0 1 2 0 4D BJ P C92755 0 0 1 3 0 0 CJ P C92778 0 0 1 1 0 4D BJ P C92790 0 1 0 2 0 2B3D CK PQ C92795 0 0 1 2 0 0 BJ P C92796 0 1 1 1 0 2B BJ PQ C92817 0 0 1 1 0 4D CJ P C92820 0 2 0 2 0 1B3D BJ PQ Covance 6329-183 3M T-6295 1714 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 6.8 1.023 6.0 8.0 1.028 6.5 10.6 1.019 7.0 8.5 1.021 6.5 7.8 1.026 6.5 9.0 1.025 6.0 14.0 1.014 6.5 8.9 1.022 7.0 8.0 1.021 6.5 9.4 1.024 6.5 MEAN S.D. N 9.1 2.00 10 1.022 .0039 10 6.5 .33 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 15.3 1.020 7.5 3.0 1.036 6.5 5.7 1.025 7.0 9.5 1.020 6.5 4.7 1.030 6.5 6.5 1.026 6.0 3.8 1.035 6.0 10.7 1.016 7.0 5.7 1.025 7.0 12.5 1.017 6.0 MEAN S.D. N 7.7 4.07 10 1.025 .0070 10 6.6 .52 10 Covance 6329-183 3M T-6295 1715 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 7.0 1.027 6.5 1.9 1.051 6.5 13.8 1.023 6.5 5.0 1.024 7.0 20.2 1.012 6.5 4.8 1.034 6.5 6.4 1.023 6.0 9.1 1.025 6.0 13.9 1.010 6.5 8.0 1.023 7.0 MEAN S.D. N 9.0 5.46 10 1.025 .0114 10 6.5 .33 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 3.3 1.040 6.5 2.8 1.036 6.5 5.7 1.023 6.5 2.5 1.033 6.0 8.2 1.019 7.5 43.5 1.008 7.0 4.2 1.028 6.5 1.5 1.041 6.5 4.2 1.037 6.5 7.1 1.034 7.0 MEAN S.D. N 8.3 12.54 10 1.030 .0105 10 6.6 .41 10 Covance 6329-183 3M T-6295 1716 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U VOL ML -------- SP GR -------- U PH -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 8.7 1.025 6.0 6.2 1.024 6.0 7.3 1.021 6.5 6.5 1.024 6.0 9.8 1.022 6.5 47.5 a 1.004 6.5 4.3 1.035 6.5 2.5 1.043 6.0 3.8 1.036 6.5 4.0 1.042 6.0 MEAN S.D. N 10.1 13.35 10 1.028 .0117 10 6.2 .26 10 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1717 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 10 - - - - - C92876 100 - - - - - C92889 10 - - - - - C92894 10 - - - - - C92899 30 - - - - - C92910 30 - - - - - C92917 100 - - - - - C92922 30 - + - + - C92923 10 - - - - - C92930 300 - - - - - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 10 - - - + - C92938 100 - - - - - C92946 30 - - - - - C92952 30 - - - - - C92954 30 - - - - - C92958 30 - - - - - C92964 100 - - - - - C92972 10 - - - - - C92985 300 - - - - - C92990 10 - - - - - Covance 6329-183 3M T-6295 1718 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 30 - - - - - C92997 100 - - - - - C93012 10 - - - - - C93022 30 - - - - - C93027 10 - - - + - C93029 30 - - - - - C93032 10 - - - - - C93042 30 - - - - - C93046 300 - - - - - C93048 10 - - - - - Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 30 - - - - - C93054 30 - - - - - C93060 10 - - - - - C93078 30 - - - - - C93081 10 - - - - - C93083 10 - - - - - C93091 30 - - - - - C93095 30 - - - - - C93097 30 - - - - - C93110 300 - - - - - Covance 6329-183 3M T-6295 1719 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U PRO MG/DL -------- U GLU -------- U KET -------- U BILI -------- U BLOOD -------- UROBILI -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 30 - - - - - C93116 10 - - - - - C93117 10 - - - - - C93120 30 - - - - - C93130 10 - - - - - C93138 0 - - - + - C93147 10 - - - - - C93154 30 - - - - - C93163 30 - - - - - C93171 30 - - - - - Covance 6329-183 3M T-6295 1720 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 0 2 1 1 0 0 BJ P C92876 0 1 1 1 0 0 BJ P C92889 0 1 1 1 0 0 BJ P C92894 0 0 1 1 0 0 BJ - C92899 0 1 1 1 0 0 BJ - C92910 0 0 2 3 0 0 BJ P C92917 0 1 0 1 0 1D BJ P C92922 4 0 1 2 0 4D1E CK P C92923 0 1 1 1 0 0 BJ P C92930 0 1 0 1 0 1B BJ - Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 3 0 1 2 0 4D CK P C92938 0 1 0 3 0 0 CK P C92946 1 1 1 1 0 3D CK P C92952 0 0 1 1 0 0 BK P C92954 0 0 0 1 0 1B CK S C92958 0 0 0 1 0 0 CJ - C92964 4 0 0 1 0 0 GL - C92972 0 0 0 1 0 1B BK - C92985 0 1 1 2 0 1B1D CK P C92990 0 1 0 1 0 1B CK - Covance 6329-183 3M T-6295 1721 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 0 0 1 1 0 0 CK P C92997 0 0 1 3 0 0 DJ P C93012 0 1 1 2 0 2B2D CK P C93022 0 0 1 1 0 0 CJ P C93027 0 1 1 1 0 0 BJ P C93029 0 0 0 1 0 0 CK P C93032 0 0 1 1 1G 0 BJ P C93042 0 0 0 1 0 1B1D CK P C93046 0 0 1 1 0 1B BJ P C93048 0 0 1 3 0 4D BJ P Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 0 0 0 1 0 0 CJ P C93054 0 1 1 1 0 0 BJ P C93060 0 1 1 1 1G 0 BJ P C93078 0 0 0 1 0 1D CJ - C93081 0 0 1 1 0 4D BJ - C93083 0 0 1 2 0 1B2D AJ P C93091 0 0 1 3 1G 0 CJ P C93095 0 1 1 1 0 0 CJ P C93097 0 1 0 1 0 1B1D CJ P C93110 0 1 1 2 1G 2B2D CK P Covance 6329-183 3M T-6295 1722 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ RBC PER HPF -------- WBC PER HPF -------- EPITH PER HPF -------- BACT PER HPF -------- CASTS PER LPF -------- CRYSTALS PER LPF1 -------- URINE APP1 -------- COMMENTS -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 0 0 1 1 0 1E BJ P C93116 0 0 1 2 0 0 BJ P C93117 1 0 1 3 0 1D CJ P C93120 0 1 1 1 0 3E CJ P C93130 0 0 0 2 0 0 BJ - C93138 0 0 0 2 0 0 AJ - C93147 0 1 1 1 0 1B1D CJ P C93154 0 0 1 1 0 3E BJ P C93163 0 1 1 1 0 1B CJ - C93171 0 0 1 1 0 3E BJ P Covance 6329-183 3M T-6295 1723 Appendix 5 Individual Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92506 C92524 a C92526 C92529 C92542 C92543 C92546 C92548 C92553 12 80 .14 .92 12 50 .41 1.71 7 28 - - 8 48 .18 1.08 9 57 .16 1.00 12 73 .23 1.40 11 71 .19 1.24 19 78 .31 1.29 13 100 .12 .95 15 61 .25 1.02 MEAN S.D. N 12 3.5 10 65 20.2 10 .22 .092 9 1.18 .258 9 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92574 C92575 C92585 C92586 a C92587 C92610 C92613 C92615 C92618 23 111 .30 1.47 13 111 .19 1.60 22 113 .27 1.38 12 75 .21 1.31 8 19 - - 28 61 .51 1.12 5 27 .21 1.15 52 192 .33 1.23 21 114 .30 1.65 7 32 .27 1.23 MEAN S.D. N 19 13.9 10 86 53.4 10 .29 .096 9 1.35 .191 9 a Unable to calculate due to inaccurate urine volume. 1724 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92632 C92646 a C92650 a C92652 C92657 a C92668 C92676 C92678 C92684 C92686 20 139 .21 1.43 3 23 - - 3 11 - - 7 54 .14 1.09 7 22 - - 11 59 .26 1.37 11 60 .18 .97 18 124 .17 1.15 34 64 .48 .90 16 90 .24 1.37 MEAN S.D. N 13 9.5 10 65 42.5 10 .24 .114 7 1.18 .211 7 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92709 C92715 C92716 a C92718 a C92725 C92731 a C92732 C92734 C92744 C92746 18 89 .28 1.38 22 60 .60 1.64 7 25 - - 4 15 - - 10 49 .24 1.20 6 21 - - 24 48 .56 1.13 16 119 .18 1.33 7 45 .30 1.93 24 66 .61 1.68 MEAN S.D. N 14 7.9 10 54 32.0 10 .40 .186 7 1.47 .289 7 a Unable to calculate due to inaccurate urine volume. 1725 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 6 40 .17 1.16 8 56 .19 1.34 62 163 .37 .98 14 49 .37 1.31 11 87 .19 1.48 12 50 .34 1.43 9 29 .36 1.15 11 66 .22 1.31 22 95 .22 .97 14 46 .35 1.14 MEAN S.D. N 17 16.4 10 68 39.1 10 .28 .086 10 1.23 .175 10 Covance 6329-183 3M T-6295 1726 Appendix 5 Individual Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 a C92925 C92926 19 90 .17 .81 31 148 .11 .50 32 212 .07 .47 27 90 .18 .59 21 81 .13 .52 8 41 .18 .92 39 160 .11 .43 5 19 - - 18 56 .17 .53 25 137 .05 .26 MEAN S.D. N 22 10.6 10 103 59.9 10 .13 .049 9 .56 .198 9 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92941 C92945 a C92950 C92953 C92959 C92964 C92968 C92969 C92976 a C92982 64 265 .10 .40 6 11 - - 6 42 .09 .65 24 116 .16 .78 7 42 .07 .42 29 94 .21 .68 29 78 .28 .75 35 126 .12 .44 9 15 - - 21 73 .18 .62 MEAN S.D. N 23 18.0 10 86 74.1 10 .15 .071 8 .59 .152 8 a Unable to calculate due to inaccurate urine volume. 1727 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 C92996 a C92998 C93004 C93006 C93008 C93023 a C93031 C93033 C93045 46 106 .18 .42 4 11 - - 15 63 .08 .32 25 118 .16 .73 24 60 .14 .35 33 78 .25 .60 4 7 - - 51 126 .21 .52 42 112 .27 .72 38 116 .28 .87 MEAN S.D. N 28 16.7 10 80 43.9 10 .20 .069 8 .57 .198 8 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 26 106 .17 .69 12 52 .17 .73 32 96 .17 .50 42 123 .34 .98 39 84 .35 .76 11 33 .20 .60 11 30 .14 .38 24 91 .27 1.02 10 36 .29 1.04 15 46 .27 .83 MEAN S.D. N 22 12.2 10 70 34.1 10 .24 .076 10 .75 .222 10 a Unable to calculate due to inaccurate urine volume. 1728 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93114 C93123 C93142 C93144 C93151 C93155 C93158 a C93159 a C93164 C93175 8 32 .23 .92 16 64 .15 .61 26 59 .25 .58 17 70 .17 .70 25 97 .13 .49 41 141 .17 .58 8 17 - - 5 13 - - 14 71 .14 .72 37 105 .24 .68 MEAN S.D. N 20 12.3 10 67 40.2 10 .18 .048 8 .66 .130 8 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1729 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 C92509 C92511 C92521 C92527 C92528 a C92532 C92535 C92537 a C92562 a 7 29 .21 .88 17 81 .32 1.55 23 43 .72 1.35 59 129 .54 1.17 13 43 .34 1.12 8 16 - - 38 70 .76 1.40 19 159 .09 .72 6 12 - - 6 19 - - MEAN S.D. N 20 17.1 10 60 50.1 10 .43 .255 7 1.17 .294 7 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 C92593 a C92598 C92600 C92612 a C92616 C92621 C92624 C92627 C92629 17 64 .29 1.08 7 16 - - 13 45 .31 1.06 38 123 .28 .91 7 21 - - 17 35 .49 1.00 57 214 .19 .73 31 69 .53 1.17 19 38 .68 1.35 41 242 .30 1.77 MEAN S.D. N 25 16.5 10 87 80.7 10 .38 .165 8 1.13 .314 8 a Unable to calculate due to inaccurate urine volume. 1730 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92636 a C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 a 3 11 - - 20 102 .14 .69 28 119 .26 1.12 49 95 .48 .92 24 107 .36 1.58 15 33 .61 1.34 32 90 .32 .91 24 194 .05 .39 20 56 .41 1.15 11 12 - - MEAN S.D. N 23 12.5 10 82 56.0 10 .33 .180 8 1.01 .372 8 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 C92713 a C92714 C92716 C92719 C92722 C92726 C92728 C92741 a C92748 28 128 .28 1.29 11 19 - - 21 59 .46 1.30 16 64 .42 1.68 45 74 .54 .89 17 40 .60 1.40 32 94 .41 1.20 30 93 .46 1.44 6 16 - - 14 60 .33 1.41 MEAN S.D. N 22 11.7 10 65 34.8 10 .44 .104 8 1.33 .226 8 a Unable to calculate due to inaccurate urine volume. 1731 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 12 102 .11 .95 41 127 .40 1.24 12 41 .44 1.52 27 79 .34 .99 7 30 .34 1.44 35 96 .42 1.15 23 74 .13 .41 34 125 .43 1.59 47 146 .36 1.11 25 66 .77 2.03 MEAN S.D. N 26 13.2 10 89 37.8 10 .37 .182 10 1.24 .438 10 Covance 6329-183 3M T-6295 1732 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92863 C92865 C92871 QS C92880 C92887 C92898 C92903 C92905 a C92906 a C92927 a 60 138 .26 .59 37 118 .26 .83 - - - - 65 135 .15 .31 37 84 .23 .52 34 99 .15 .45 43 87 .28 .57 6 12 - - 5 13 - - 3 8 - - MEAN S.D. N 32 23.2 9 77 53.0 9 .22 .058 6 .54 .172 6 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92935 C92940 C92944 a C92948 C92956 C92962 a C92967 C92978 C92986 C92987 65 142 .21 .45 9 20 .17 .37 11 16 - - 28 66 .20 .46 21 52 .26 .64 13 20 - - 32 101 .22 .70 27 87 .31 1.00 16 82 .10 .53 47 118 .15 .37 MEAN S.D. N 27 17.6 10 70 43.6 10 .20 .065 8 .56 .212 8 a Unable to calculate due to inaccurate urine volume. 1733 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 9 40 .17 .75 66 114 .24 .41 35 123 .18 .62 49 73 .39 .58 25 54 .51 1.10 39 91 .16 .38 21 94 .10 .47 48 168 .16 .57 72 171 .19 .46 67 129 .48 .92 MEAN S.D. N 43 21.2 10 106 44.1 10 .26 .146 10 .63 .234 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93051 C93064 C93071 a C93076 C93078 a C93085 C93089 C93090 C93093 C93109 31 89 .27 .77 23 46 .37 .74 6 12 - - 17 43 .33 .84 7 11 - - 26 56 .31 .68 22 72 .47 1.55 7 23 .29 .95 10 29 .26 .75 10 42 .18 .78 MEAN S.D. N 16 9.1 10 42 25.2 10 .31 .085 8 .88 .281 8 a Unable to calculate due to inaccurate urine volume. 1734 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 C93113 C93120 C93127 C93135 a C93143 C93155 C93162 C93169 C93178 a 36 58 .49 .78 13 43 .16 .52 46 66 .37 .53 20 32 .76 1.22 6 16 - - 50 86 .30 .52 143 252 .21 .38 20 50 .26 .66 31 81 .22 .58 4 10 - - MEAN S.D. N 37 40.5 10 69 68.9 10 .35 .197 8 .65 .259 8 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1735 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 10 113 .04 .51 59 288 .27 1.32 16 78 .26 1.26 15 81 .28 1.54 24 124 .37 1.91 15 110 .28 2.02 9 68 .20 1.51 24 154 .18 1.16 18 60 .42 1.41 30 81 .61 1.65 MEAN S.D. N 22 14.5 10 116 67.1 10 .29 .153 10 1.43 .422 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 16 96 .20 1.17 18 76 .19 .81 16 113 .17 1.20 13 89 .17 1.16 12 62 .18 .91 8 46 .23 1.30 9 71 .09 .71 16 55 .28 .98 42 124 .46 1.36 7 40 .24 1.38 MEAN S.D. N 16 10.0 10 77 28.1 10 .22 .098 10 1.10 .234 10 Covance 6329-183 3M T-6295 1736 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92633 C92644 C92647 TE C92658 C92660 C92667 C92673 C92679 C92686 C92687 40 94 .68 1.61 34 117 .36 1.24 - - - - 28 110 .32 1.26 25 125 .23 1.15 18 53 .39 1.16 18 105 .19 1.09 14 59 .24 1.03 10 75 .12 .90 30 95 .63 1.99 MEAN S.D. N 24 9.9 9 93 25.3 9 .35 .192 9 1.27 .333 9 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92696 C92698 a C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 a 22 62 .35 1.00 2 11 - - 24 113 .27 1.27 17 63 .39 1.45 26 158 .22 1.36 13 56 .19 .81 17 70 .34 1.41 60 228 .49 1.85 19 110 .11 .62 9 20 - - MEAN S.D. N 21 15.5 10 89 65.7 10 .30 .122 8 1.22 .394 8 a Unable to calculate due to inaccurate urine volume. 1737 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 C92761 C92764 C92773 C92783 C92788 a C92791 C92803 C92804 C92820 a 23 136 .38 2.24 38 116 .27 .81 142 414 .21 .62 30 91 .48 1.47 67 187 .59 1.65 6 25 - - 83 153 .86 1.59 13 58 .25 1.13 47 192 .23 .94 6 18 - - MEAN S.D. N 46 42.4 10 139 114.5 10 .41 .226 8 1.31 .532 8 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1738 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92876 C92878 C92888 C92906 C92916 C92917 C92921 a C92922 a C92923 C92928 54 227 .10 .41 9 61 .09 .64 32 90 .22 .63 5 18 .13 .48 10 61 .14 .83 40 142 .21 .74 1 9 - - 3 14 - - 17 62 .22 .81 8 27 .03 .11 MEAN S.D. N 18 18.0 10 71 68.2 10 .14 .070 8 .58 .241 8 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 a C92979 C92989 17 75 .23 1.02 35 154 .21 .94 8 30 .16 .61 12 70 .10 .56 5 22 .11 .50 13 80 .15 .92 9 71 .13 1.05 2 14 - - 25 66 .24 .63 19 163 .10 .85 MEAN S.D. N 14 9.9 10 74 50.2 10 .16 .055 9 .79 .212 9 a Unable to calculate due to inaccurate urine volume. 1739 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 4 20 .17 .84 42 108 .14 .37 13 31 .28 .67 29 86 .26 .78 11 46 .14 .59 54 182 .22 .73 6 82 .04 .57 6 30 .12 .58 10 30 .30 .89 39 86 .24 .53 MEAN S.D. N 21 18.1 10 70 49.9 10 .19 .083 10 .66 .158 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 a C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 3 10 - - 54 152 .30 .84 27 80 .26 .76 5 23 .15 .70 3 17 .12 .67 11 44 .21 .84 21 48 .52 1.20 38 180 .06 .31 13 56 .26 1.12 5 58 .10 1.13 MEAN S.D. N 18 17.2 10 67 56.7 10 .22 .139 9 .84 .280 9 a Unable to calculate due to inaccurate urine volume. 1740 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 5 20 .12 .49 36 103 .21 .60 133 319 .16 .38 7 38 .10 .52 28 91 .32 1.05 32 71 .36 .80 3 24 .12 .98 20 100 .18 .90 28 71 .44 1.12 32 124 .12 .48 MEAN S.D. N 32 37.4 10 96 85.7 10 .21 .119 10 .73 .270 10 Covance 6329-183 3M T-6295 1741 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 47 171 .63 2.27 20 71 .38 1.35 10 37 .30 1.13 36 116 .35 1.11 59 228 .38 1.48 16 130 .11 .88 38 236 .17 1.09 45 160 .40 1.44 28 105 .27 1.00 22 80 .36 1.30 MEAN S.D. N 32 15.5 10 133 65.6 10 .34 .141 10 1.30 .390 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 a C92625 C92629 18 174 .18 1.77 32 108 .61 2.05 46 160 .44 1.54 17 108 .27 1.71 10 68 .28 1.90 12 107 .19 1.71 61 378 .13 .79 4 22 - - 20 245 .13 1.64 9 70 .22 1.74 MEAN S.D. N 23 18.2 10 144 103.4 10 .27 .158 9 1.65 .354 9 a Unable to calculate due to inaccurate urine volume. 1742 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 24 111 .21 .97 19 96 .21 1.08 9 78 .09 .79 15 73 .24 1.18 11 82 .27 2.01 19 56 .57 1.68 10 62 .26 1.64 44 128 .64 1.86 31 144 .32 1.47 32 129 .32 1.29 MEAN S.D. N 21 11.4 10 96 30.6 10 .31 .168 10 1.40 .401 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 5 61 .14 1.74 58 335 .23 1.34 19 127 .16 1.08 29 138 .26 1.24 12 99 .15 1.25 26 146 .29 1.61 32 166 .26 1.36 39 143 .44 1.63 77 380 .25 1.22 24 84 .48 1.66 MEAN S.D. N 32 21.5 10 168 105.3 10 .27 .115 10 1.41 .228 10 Covance 6329-183 3M T-6295 1743 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 85 155 .80 1.46 51 135 .56 1.48 20 135 .06 .40 54 130 .66 1.59 48 192 .32 1.29 31 111 .54 1.93 23 111 .37 1.78 58 215 .53 1.98 41 119 .58 1.69 MEAN S.D. N 46 20.0 9 145 36.4 9 .49 .215 9 1.51 .473 9 Covance 6329-183 3M T-6295 1744 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 30 99 .20 .67 51 167 .41 1.34 37 91 .39 .96 29 100 .25 .85 74 186 .58 1.45 25 144 .22 1.30 32 85 .45 1.19 53 209 .47 1.86 37 129 .30 1.03 37 136 .35 1.28 MEAN S.D. N 40 14.8 10 135 42.4 10 .36 .122 10 1.19 .337 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 40 99 .61 1.51 28 228 .08 .68 59 119 .34 .68 16 94 .15 .89 20 140 .09 .66 35 182 .23 1.18 17 166 .06 .63 22 70 .24 .75 26 152 .15 .87 16 70 .20 .88 MEAN S.D. N 28 13.6 10 132 51.3 10 .22 .163 10 .87 .278 10 Covance 6329-183 3M T-6295 1745 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 14 125 .10 .88 32 304 .06 .58 24 119 .33 1.64 22 126 .11 .63 9 56 .18 1.13 24 182 .12 .87 39 176 .25 1.13 21 133 .19 1.21 15 68 .21 .95 26 111 .21 .89 MEAN S.D. N 23 8.8 10 140 69.9 10 .18 .081 10 .99 .307 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 59 254 .19 .84 47 261 .13 .73 53 146 .30 .83 18 139 .04 .35 40 94 .33 .77 12 24 .52 1.04 31 155 .13 .65 34 283 .05 .42 37 211 .16 .89 39 153 .28 1.09 MEAN S.D. N 37 14.5 10 172 80.9 10 .21 .147 10 .76 .239 10 Covance 6329-183 3M T-6295 1746 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93112 C93116 C93117 C93120 C93130 C93138 a C93147 C93154 C93163 C93171 27 149 .23 1.30 21 133 .13 .82 40 130 .29 .95 14 102 .09 .66 73 120 .72 1.18 10 23 - - 60 158 .26 .68 61 300 .15 .75 48 246 .18 .93 64 275 .26 1.10 MEAN S.D. N 42 22.7 10 164 85.4 10 .26 .186 9 .93 .226 9 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1747 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume V of IX Page 1748 of 4068 Covance 6329-183 3M T-6295 VOLUME V OF IX CONTENTS Page APPENDIX 6............................................................................................................ 1750 Individual Anatomic Pathology Data ..................................................................... 1751 1749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 1750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92501 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 850.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:33 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0462 .0054 % .0207 WEIGHT TAKEN RT ADRENAL (AD1) .0172 .0020 % .0077 WEIGHT TAKEN BRAIN (BR) 2.2353 .2629 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.4173 .2843 % 1.0814 WEIGHT TAKEN RT KIDNEY (KD1) 2.4867 .2925 % 1.1125 WEIGHT TAKEN LIVER (LI) 25.4814 2.9971 % 11.3995 WEIGHT TAKEN LUNG (LU) 2.3481 .2762 % 1.0505 WEIGHT TAKEN SPLEEN (SP) 1.1213 .1319 % .5016 WEIGHT TAKEN LF TESTIS (TE0) 1.8183 .2139 % .8134 WEIGHT TAKEN RT TESTIS (TE1) 1.7568 .2066 % .7859 WEIGHT TAKEN LF THYROID/PARA (TT0) .0346 .0041 % .0155 WEIGHT TAKEN RT THYROID/PARA (TT1) .0258 .0030 % .0115 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 1751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92501 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 850.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:33 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 3 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92501 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 850.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:33 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92502 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 495.7 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:45 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT NECROPSY: NOT APPLICABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -HYALINE DROPLETS, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 1754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 5 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92502 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 495.7 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:45 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 25 X 14 X 3 >UNREMARKABLE MM >NOTE:>SEE COAGULATING GLAND CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT COAGULATING GL (CG) : -SECRETORY MATERIAL, INCREASED,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED DRY MATERIAL -ANIMAL THIN -------------------------------------------- 1755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 6 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92502 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 495.7 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:45 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 7 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-PROXIMAL; WEEK 72; 15 X LIVER (LI) : 10MM; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL >MASS(ES); TAIL-PROXIMAL; WEEK 105; 15 KIDNEY (KD) : X 10MM BOTTOM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 18 X 9 X 5 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE 25 X 11 X 10 MM) >NOTE:>UNILATERAL THYMUS (TH) : THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: >UNREMARKABLE SINGLE MASS, 30 X 26 X 15 MM, RED, >NOTE:>SEE CAVITY, THORACIC FOR DARK RED, TAN, AND FIRM; SAME ON CUT DIAGNOSIS SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED 1757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 8 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM PROSTATE (PR) : PROSTATE (PR) : -SMALL; 10 MM IN DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 19 X 12 X 3 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT COAGULATING GL (CG) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT 1758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 9 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : -MASS(ES); NEAR BASE OF TAIL: TWO MASSES, EACH IS 12 X 7 X 5 MM, EACH IS TAN AND FIRM; SAME ON CUT SECTIONS; COLLECTED; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; LEFT: DARK RED (CAP -HEMORRHAGE,-PRESENT 23) -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL CAVITY, THORACIC (TA) : -B-AORTIC BODY TUMOR,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN -------------------------------------------- 1759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 10 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 11 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92504 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 650.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0300 .0046 % .0143 WEIGHT TAKEN RT ADRENAL (AD1) .0306 .0047 % .0146 WEIGHT TAKEN BRAIN (BR) 2.0966 .3226 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.2133 .3405 % 1.0557 WEIGHT TAKEN RT KIDNEY (KD1) 2.1217 .3264 % 1.0120 WEIGHT TAKEN LIVER (LI) 16.8986 2.5998 % 8.0600 WEIGHT TAKEN LUNG (LU) 2.0480 .3151 % .9768 WEIGHT TAKEN SPLEEN (SP) .8682 .1336 % .4141 WEIGHT TAKEN LF TESTIS (TE0) 2.1700 .3338 % 1.0350 WEIGHT TAKEN RT TESTIS (TE1) 2.2147 .3407 % 1.0563 WEIGHT TAKEN LF THYROID/PARA (TT0) .0181 .0028 % .0086 WEIGHT TAKEN RT THYROID/PARA (TT1) .0171 .0026 % .0082 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 1761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 12 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92504 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 650.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 1762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 13 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92504 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 650.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 1764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 15 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 9 X 7 X 6 MM (LEFT IS 7 -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM X 3 X 3 MM) PRESENT -DIFFUSELY DARK; RIGHT: DARK BROWN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATELY SEVERE DUODENUM (DU) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE JEJUNUM (JE) : JEJUNUM (JE) : -THICKENED WALL; DIFFUSE >UNREMARKABLE ILEUM (IL) : ILEUM (IL) : -THICKENED WALL; DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE CECUM (CE) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE COLON (CO) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE >NOTE:>EOSINOPHILS PREDOMINATE RECTUM (RE) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 16 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 17 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 18 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92506 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:54 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.8663 3.2924 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 19 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92506 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:54 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 1769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 20 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92507 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 636.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:49 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0224 .0035 % .0094 WEIGHT TAKEN RT ADRENAL (AD1) .0247 .0039 % .0104 WEIGHT TAKEN BRAIN (BR) 2.3724 .3727 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.1481 .3375 % .9055 WEIGHT TAKEN RT KIDNEY (KD1) 2.1112 .3317 % .8899 WEIGHT TAKEN LIVER (LI) 20.1131 3.1600 % 8.4780 WEIGHT TAKEN LUNG (LU) 2.1448 .3370 % .9041 WEIGHT TAKEN SPLEEN (SP) .9408 .1478 % .3966 WEIGHT TAKEN LF TESTIS (TE0) 1.8775 .2950 % .7914 WEIGHT TAKEN RT TESTIS (TE1) 1.7754 .2789 % .7484 WEIGHT TAKEN LF THYROID/PARA (TT0) .0301 .0047 % .0127 WEIGHT TAKEN RT THYROID/PARA (TT1) .0303 .0048 % .0128 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 1770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 21 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92507 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 636.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:49 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 22 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92507 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 636.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:49 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 23 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92508 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 8:23 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 1773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 24 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92508 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 8:23 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE >AUTOLYTIC WHITE, FIRM CALCULI, 6 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL -------------------------------------------- 1774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 25 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92508 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 8:23 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 26 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92509 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:18 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0322 .0066 % .0140 WEIGHT TAKEN RT ADRENAL (AD1) .0308 .0063 % .0134 WEIGHT TAKEN BRAIN (BR) 2.3068 .4698 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.7786 .3622 % .7710 WEIGHT TAKEN RT KIDNEY (KD1) 1.8602 .3789 % .8064 WEIGHT TAKEN LIVER (LI) 14.5106 2.9553 % 6.2904 WEIGHT TAKEN LUNG (LU) 1.7988 .3664 % .7798 WEIGHT TAKEN SPLEEN (SP) .8923 .1817 % .3868 WEIGHT TAKEN LF TESTIS (TE0) 1.8453 .3758 % .7999 WEIGHT TAKEN RT TESTIS (TE1) 1.8482 .3764 % .8012 WEIGHT TAKEN LF THYROID/PARA (TT0) .0131 .0027 % .0057 WEIGHT TAKEN RT THYROID/PARA (TT1) .0128 .0026 % .0055 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92509 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:18 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 28 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92510 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 1778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 29 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92510 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 30 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92510 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 31 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92511 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 506.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0348 .0069 % .0169 WEIGHT TAKEN RT ADRENAL (AD1) .0213 .0042 % .0103 WEIGHT TAKEN BRAIN (BR) 2.0610 .4067 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.9719 .3891 % .9568 WEIGHT TAKEN RT KIDNEY (KD1) 1.9652 .3878 % .9535 WEIGHT TAKEN LIVER (LI) 16.1948 3.1955 % 7.8577 WEIGHT TAKEN LUNG (LU) 2.1768 .4295 % 1.0562 WEIGHT TAKEN SPLEEN (SP) .7816 .1542 % .3792 WEIGHT TAKEN LF TESTIS (TE0) 1.9467 .3841 % .9445 WEIGHT TAKEN RT TESTIS (TE1) 1.9871 .3921 % .9641 WEIGHT TAKEN LF THYROID/PARA (TT0) .0109 .0022 % .0053 WEIGHT TAKEN RT THYROID/PARA (TT1) .0139 .0027 % .0067 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE RED PINPOINT FOCI CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 1781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 32 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92511 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 506.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 33 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92512 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 654.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0380 .0058 % .0157 WEIGHT TAKEN RT ADRENAL (AD1) .0269 .0041 % .0111 WEIGHT TAKEN BRAIN (BR) 2.4142 .3691 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.4170 .3695 % 1.0012 WEIGHT TAKEN RT KIDNEY (KD1) 2.3030 .3521 % .9539 WEIGHT TAKEN LIVER (LI) 19.0310 2.9095 % 7.8829 WEIGHT TAKEN LUNG (LU) 2.0416 .3121 % .8457 WEIGHT TAKEN SPLEEN (SP) .8649 .1322 % .3583 WEIGHT TAKEN LF TESTIS (TE0) 2.4547 .3753 % 1.0168 WEIGHT TAKEN RT TESTIS (TE1) 1.8193 .2781 % .7536 WEIGHT TAKEN LF THYROID/PARA (TT0) .0165 .0025 % .0068 WEIGHT TAKEN RT THYROID/PARA (TT1) .0216 .0033 % .0089 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL 1783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 34 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92512 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 654.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 35 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92512 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 654.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 36 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92513 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 1786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 37 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92513 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 38 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92513 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 39 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92514 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1041.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:40 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT 1789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 40 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92514 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1041.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:40 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; ALL REGIONS DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT COLON (CO) : -PARASITISM,-PRESENT RECTUM (RE) : -PARASITISM,-PRESENT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 41 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92514 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1041.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:40 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), KIDNEY (KD), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 42 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92515 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/12/00 13:12 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT 1792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 43 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92515 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/12/00 13:12 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 44 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92515 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/12/00 13:12 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 45 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92516 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 326 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 7:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -CONGESTION,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -CONGESTION,-PRESENT LN, MESENTERIC (MS) : >TISSUE MISSING MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -CONGESTION,-PRESENT HEART (HT) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FIBROSIS,-MINIMAL 1795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 46 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92516 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 326 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 7:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 47 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92516 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 326 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 7:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 48 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92517 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 913.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0325 .0036 % .0138 WEIGHT TAKEN RT ADRENAL (AD1) .0365 .0040 % .0155 WEIGHT TAKEN BRAIN (BR) 2.3607 .2584 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.6614 .2913 % 1.1274 WEIGHT TAKEN RT KIDNEY (KD1) 2.6775 .2930 % 1.1342 WEIGHT TAKEN LIVER (LI) 24.8245 2.7169 % 10.5157 WEIGHT TAKEN LUNG (LU) 2.5656 .2808 % 1.0868 WEIGHT TAKEN SPLEEN (SP) 1.1036 .1208 % .4675 WEIGHT TAKEN LF TESTIS (TE0) 1.8990 .2078 % .8044 WEIGHT TAKEN RT TESTIS (TE1) 1.9440 .2128 % .8235 WEIGHT TAKEN LF THYROID/PARA (TT0) .0289 .0032 % .0122 WEIGHT TAKEN RT THYROID/PARA (TT1) .0308 .0034 % .0130 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 1798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 49 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92517 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 913.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : LUNG (LU) : -DARK FOCUS(I)/AREA(S); LEFT LOBE: -CONGESTION,-PRESENT SINGLE BLACK FOCUS, 2 MM IN DIAMETER PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 50 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92517 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 913.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 51 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92518 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 841.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SURFACE OF RIGHT MEDIAN LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DARK RED AREA, 4 MM IN DIAMETER; EOSINOPHILIC CELL,-MINIMAL COLLECTED ON ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 1801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 52 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92518 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 841.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT MID -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 1802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92518 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 841.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 54 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92519 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 10:37 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 X 4 X 3 MM (LEFT IS 3 -UNILATERALLY EXAMINED,-PRESENT X 2 X 2 MM) ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 1804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 55 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92519 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 10:37 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 6 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GREY, AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 1805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 56 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92519 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 10:37 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 57 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; LIMB-HIND- LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: NOT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; SWOLLEN; DIGIT(S)-HIND- BASOPHILIC,-MINIMAL RIGHT; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : KIDNEY (KD) : APPLICABLE -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-MODERATE -LARGE; BILATERAL: EACH ARE 33 X 23 X -MINERALIZATION, TUBULAR,-MINIMAL 10 MM; BOTH ARE SOFT -ECTASIA, TUBULAR,-SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -FIBROSIS, CORTICAL,-SLIGHT SPLEEN (SP) : SPLEEN (SP) : -ADHESION(S); CRANIAL END ADHERED TO -FIBROSIS, CAPSULAR,-PRESENT DIAPHRAGM: MULTIPLE GRAY, FIBROUS ADHESIONS; COLLECTED INTACT PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; ALONG DUODENUM: UP TO -INFILTRATE, MACROPHAGE, PIGMENTED,- 5 MM IN DIAMETER; COLLECTED INTACT MINIMAL WITH DUODENUM -INFLAMMATION, VASCULAR,-MODERATE -THROMBUS,-PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATE 1807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 58 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-SLIGHT -MINERALIZATION,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 6 X 4 X 3 MM (LEFT IS 5 -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM X 3 X 3 MM) PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN PINPOINT FOCI DUODENUM (DU) : -INFLAMMATION, VASCULAR,-MODERATE CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : URINARY BLADDER (UB) : -DIFFUSELY RED; DARK RED -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 1808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 59 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND NODULAR SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; DISTENDED TO 35 X 25 X 15 MM WITH A DARK GREEN FLUID -------------------------------------------- 1809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 60 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 61 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92521 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 467.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0329 .0070 % .0146 WEIGHT TAKEN RT ADRENAL (AD1) .0340 .0073 % .0151 WEIGHT TAKEN BRAIN (BR) 2.2509 .4813 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.0805 .4448 % .9243 WEIGHT TAKEN RT KIDNEY (KD1) 2.0278 .4336 % .9009 WEIGHT TAKEN LIVER (LI) 16.3067 3.4866 % 7.2445 WEIGHT TAKEN LUNG (LU) 1.8853 .4031 % .8376 WEIGHT TAKEN SPLEEN (SP) .7612 .1628 % .3382 WEIGHT TAKEN LF TESTIS (TE0) 1.9720 .4216 % .8761 WEIGHT TAKEN RT TESTIS (TE1) 1.8773 .4014 % .8340 WEIGHT TAKEN LF THYROID/PARA (TT0) .0130 .0028 % .0058 WEIGHT TAKEN RT THYROID/PARA (TT1) .0164 .0035 % .0073 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 62 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92521 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 467.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) ------------------------------------------------------------------------------------------------------------------------------------ 1812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 63 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92522 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/29/00 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 639.8 GRAMS DATE AND TIME OF NECROPSY: 02/29/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; VENTRAL- -COMPRESSION, VENTRAL,-PRESENT ABDOMINAL; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -DARK FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT MULTIPLE DARK RED AREAS, UP TO 7 X 5 -VACUOLATION, HEPATOCELLULAR, NECROPSY: OBSERVED MM, COLLECTED ON ROUTINE SECTIONS PERIPORTAL,-SLIGHT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -HYPERPLASIA, BILE DUCT,-MINIMAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 1813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 64 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92522 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/29/00 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 639.8 GRAMS DATE AND TIME OF NECROPSY: 02/29/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT BROWN, AND FIRM SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); VENTRAL ABDOMEN, -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT OVERLYING RIGHT PREPUTIAL GLAND: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE BROWN CRUSTED AREA, 2 X 3 MM MODERATELY SEVERE (CAP 22) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE HIND FEET: SINGLE DRY, RED, AND TAN -ULCERATION,-MODERATE AREA ON EACH, LEFT IS 11 X 7 MM, RIGHT IS 7 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -------------------------------------------- 1814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 65 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92522 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/29/00 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 639.8 GRAMS DATE AND TIME OF NECROPSY: 02/29/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 66 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92523 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0280 .0036 % .0110 WEIGHT TAKEN RT ADRENAL (AD1) .0276 .0036 % .0108 WEIGHT TAKEN BRAIN (BR) 2.5515 .3309 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.2588 .2930 % .8853 WEIGHT TAKEN RT KIDNEY (KD1) 2.4189 .3137 % .9480 WEIGHT TAKEN LIVER (LI) 19.4596 2.5239 % 7.6267 WEIGHT TAKEN LUNG (LU) 1.8566 .2408 % .7277 WEIGHT TAKEN SPLEEN (SP) 1.0752 .1395 % .4214 WEIGHT TAKEN LF TESTIS (TE0) 1.8292 .2373 % .7169 WEIGHT TAKEN RT TESTIS (TE1) 1.7216 .2233 % .6747 WEIGHT TAKEN LF THYROID/PARA (TT0) .0214 .0028 % .0084 WEIGHT TAKEN RT THYROID/PARA (TT1) .0273 .0035 % .0107 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MINIMAL 1816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 67 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92523 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 68 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92523 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 69 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92524 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 366.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:59 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 14.3739 3.9212 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 70 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92524 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 366.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:59 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 1820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 71 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92525 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 474.0 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:54 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 61; BRAIN (BR) : BRAIN (BR) : 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED KIDNEY (KD) : >SKIN & PELAGE; ROUGH HAIR COAT; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE MINIMAL >EXCRETION; NO FECES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >BEHAVIOR; HYPOACTIVE; MODERATE; MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 17 X 12 X 10 MM, TAN, -B-ADENOMA,-NEOPLASM PRESENT BROWN, DARK RED AND SOFT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT 1821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 72 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92525 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 474.0 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:54 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -SMALL; 10 X 10 X 6 MM -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 8 X 2 -SECRETION, DECREASED,-PRESENT MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); MID ABDOMINAL REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 17 X 12 X 10 MM, TAN, >NOTE:>SEE SKIN FOR HISTOLOGIC BROWN AND FIRM; CUT SURFACE CONTAINS A DIAGNOSIS TAN AND CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 73 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92525 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 474.0 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:54 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 74 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92526 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 325.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:33 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.0558 3.7028 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 75 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92526 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 325.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:33 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 1825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 76 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92527 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 561.4 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED -M-ASTROCYTOMA,-NEOPLASM PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >APPEARANCE; LIMITED USE; LIMB-HIND- -NECROSIS, COAGULATIVE,-MINIMAL RIGHT; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT -DEPLETION, LYMPHOCYTIC (GENERALLY POSITIVELY IDENTIFIED; POSSIBLE COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- REMNANTS COLLECTED SLIGHT 1826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 77 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92527 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 561.4 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 78 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92527 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 561.4 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 79 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92528 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 430.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:32 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0347 .0081 % .0154 WEIGHT TAKEN RT ADRENAL (AD1) .0315 .0073 % .0140 WEIGHT TAKEN BRAIN (BR) 2.2548 .5235 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.5393 .3574 % .6827 WEIGHT TAKEN RT KIDNEY (KD1) 1.6264 .3776 % .7213 WEIGHT TAKEN LIVER (LI) 14.8186 3.4406 % 6.5720 WEIGHT TAKEN LUNG (LU) 1.8216 .4229 % .8079 WEIGHT TAKEN SPLEEN (SP) .6689 .1553 % .2967 WEIGHT TAKEN LF TESTIS (TE0) 1.4100 .3274 % .6253 WEIGHT TAKEN RT TESTIS (TE1) 1.4018 .3255 % .6217 WEIGHT TAKEN LF THYROID/PARA (TT0) .0105 .0024 % .0047 WEIGHT TAKEN RT THYROID/PARA (TT1) .0112 .0026 % .0050 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 80 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92528 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 430.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:32 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 81 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92529 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 272.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:39 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.2021 3.7449 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 82 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92529 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 272.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:39 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 1832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 83 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92530 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >RESPIRATION; LABORED; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >RESPIRATION; AUDIBLE; VERIFIED AT -RED FOCUS(I)/AREA(S); PAPILLARY -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE PROCESS: SINGLE RED AREA, 3 MM IN -INFILTRATE, MACROPHAGE, PIGMENTED,- >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED DIAMETER; COLLECTED MINIMAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- ENTIRE; VERIFIED AT NECROPSY: OBSERVED SLIGHT >EXCRETION; FEW FECES; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT 1833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 84 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92530 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 7 MM, RED, TAN, AND -B-ADENOMA,-NEOPLASM PRESENT SOFT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED AND DRY MATERIAL -------------------------------------------- 1834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 85 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92530 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 86 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92531 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 522.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 8:06 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EXCRETION; NO FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-MODERATE >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -PIGMENT, TUBULAR,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED SPLEEN (SP) : AT NECROPSY: NOT OBSERVED -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -MINERALIZATION,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 7 MM, RED, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT 1836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 87 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92531 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 522.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 8:06 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 6 X 4 MM -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -------------------------------------------- 1837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 88 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92531 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 522.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 8:06 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 89 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92532 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0310 .0053 % .0145 WEIGHT TAKEN RT ADRENAL (AD1) .0334 .0057 % .0156 WEIGHT TAKEN BRAIN (BR) 2.1402 .3681 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.2086 .3799 % 1.0320 WEIGHT TAKEN RT KIDNEY (KD1) 2.0147 .3465 % .9414 WEIGHT TAKEN LIVER (LI) 17.2925 2.9743 % 8.0799 WEIGHT TAKEN LUNG (LU) 1.9369 .3331 % .9050 WEIGHT TAKEN SPLEEN (SP) .7755 .1334 % .3623 WEIGHT TAKEN LF TESTIS (TE0) 1.5752 .2709 % .7360 WEIGHT TAKEN RT TESTIS (TE1) 1.6085 .2767 % .7516 WEIGHT TAKEN LF THYROID/PARA (TT0) .0336 .0058 % .0157 WEIGHT TAKEN RT THYROID/PARA (TT1) .0310 .0053 % .0145 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 90 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92532 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; RIGHT: DARK RED (CAP >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 21) -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 1840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 91 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 69; 35 X 40MM; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK SURFACE OF RIGHT MEDIAN LOBE: MULTIPLE EOSINOPHILIC CELL,-SLIGHT 71; 30 X 40MM; VERIFIED AT NECROPSY: RED AREAS, UP TO 3 MM IN DIAMETER; -DEGENERATION, CYSTIC,-MINIMAL OBSERVED SOME ARE SUNKEN WITH A STELLATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >SKIN & PELAGE; SORE/SCAB; HEAD- PATTERN; COLLECTED ON ROUTINE SECTIONS -HYPERPLASIA, BILE DUCT,-SLIGHT MAXILLARY-RIGHT; VERIFIED AT NECROPSY: -FIBROSIS, PERIBILIARY,-MINIMAL OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -HYALINE DROPLETS, TUBULAR EPITHELIUM,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERPLASIA, CORTICAL,-SLIGHT -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE -N-FIBROSARCOMA,-NEOPLASM PRESENT 1841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 92 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT SKIN (SK) : -M-FIBROSARCOMA,- 2 NEOPLASMS PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM >UNREMARKABLE HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; -HYPERPLASIA, LYMPHOPLASMACYTIC,- COLLECTED MODERATE MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS 1842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 93 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 44 X 40 X 18 MM, RED, TAN, AND FIRM; SAME ON CUT SECTION; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT CERVICAL REGION: SINGLE MASS, 38 X 30 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SECTION; COLLECTED; REGIONAL LN (SEE MANDIBULAR); OVERLYING SKIN HAS A SINGLE TAN ABRADED AREA; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- 1843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 94 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 1844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 95 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92534 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 629.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:49 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0341 .0054 % .0154 WEIGHT TAKEN RT ADRENAL (AD1) .0350 .0056 % .0158 WEIGHT TAKEN BRAIN (BR) 2.2118 .3514 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.1935 .3485 % .9917 WEIGHT TAKEN RT KIDNEY (KD1) 2.3288 .3699 % 1.0529 WEIGHT TAKEN LIVER (LI) 17.9201 2.8467 % 8.1020 WEIGHT TAKEN LUNG (LU) 1.9706 .3130 % .8909 WEIGHT TAKEN SPLEEN (SP) .9603 .1525 % .4342 WEIGHT TAKEN LF TESTIS (TE0) 1.0650 .1692 % .4815 WEIGHT TAKEN RT TESTIS (TE1) 2.0846 .3312 % .9425 WEIGHT TAKEN LF THYROID/PARA (TT0) .0242 .0038 % .0109 WEIGHT TAKEN RT THYROID/PARA (TT1) .0244 .0039 % .0110 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 1845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 96 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92534 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 629.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:49 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 20 X 12 X 6 MM (FOR -ATROPHY/DEGENERATION,-MODERATE COMPARISON, LEFT IS 14 X 10 X 10 MM); -GRANULOMA, SPERM,-PRESENT SOFT THYMUS (TH) : THYMUS (TH) : >NOTE:>TRIMMING COMMENT: THYMUS MISSING >TISSUE MISSING AT TIME OF TRIMMING URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; TRIMMING COMMENT: BILATERAL: -HEMORRHAGE,-PRESENT DIFFUSELY RED AND TAN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 1846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 97 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92534 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 629.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:49 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 98 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; DISCOLORED FECES; RED IN BRAIN (BR) : BRAIN (BR) : COLOR; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE APPLICABLE LIVER (LI) : >APPEARANCE; TREMORS; LIMBS-ALL; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-SLIGHT >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NONFORMED FECES; VERIFIED MINIMAL AT NECROPSY: NOT APPLICABLE -PIGMENT, TUBULAR,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED SPLEEN (SP) : SPLEEN (SP) : AT NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); PARIETAL -PIGMENT, INCREASED,-SLIGHT SURFACE OF CRANIAL END: SINGLE GRAY AREA, 15 X 10 MM -ADHESION(S); PARIETAL SURFACE ATTACHED TO GREATER CURVATURE OF STOMACH: MULTIPLE GRAY FIBROUS ADHESIONS; COLLECTED INTACT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 1848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 99 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 15 X 5 X 3 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE 22 X 15 X 10 MM); LEFT IS SOFT >NOTE:>UNILATERAL BONE, STERNUM (SB) : -HYPERPLASIA, BONE,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 3 MM, -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE RED, DARK RED, TAN, AND FIRM -NECROSIS,-SEVERE STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -EDEMA,-MODERATE DARK BROWN, SLIGHTLY ERODED FOCI, UP -EROSION,-MODERATE TO 3 MM IN DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE ILEUM (IL) : ILEUM (IL) : -DIFFUSELY RED; MUCOSA -HEMORRHAGE,-PRESENT -NECROSIS,-SLIGHT CECUM (CE) : CECUM (CE) : -DIFFUSELY RED; MUCOSA >UNREMARKABLE 1849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 100 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : RECTUM (RE) : -DIFFUSELY RED; MUCOSA -NECROSIS,-SEVERE EPIDIDYMIDES (EP) : -GRANULOMA, SPERM,-PRESENT CAUSE OF DEATH (CD) : -GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMA- TION,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -MASS(ES); WITHIN ADIPOSE TISSUE, NEAR >NOT REQUIRED TO BE EXAMINED FOR ANIMAL LEFT TESTIS: SINGLE MASS, 10 X 6 X 6 >NOTE:>SEE EPIDIDYMIDES FOR DIAGNOSIS MM, YELLOW AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (LUMBAR) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TACT: DARK BROWN AND DARK RED FLUID MATERIAL -------------------------------------------- 1850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 101 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 102 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92536 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 808.5 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 8:30 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >RESPIRATION; AUDIBLE; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; SWOLLEN; NOSE; VERIFIED AT SLIGHT NECROPSY: OBSERVED SPLEEN (SP) : >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -HEMATOPOIESIS, EXTRAMEDULLARY, VERIFIED AT NECROPSY: NOT APPLICABLE INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL 1852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 103 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92536 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 808.5 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 8:30 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE TAN FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT SKIN (SK) : SKIN (SK) : -RED FOCUS(I)/AREA(S); SUBCUTANEOUS -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT TISSUE SURROUNDING MUZZLE: MULTIPLE -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL INDISTINCT RED AND DARK RED AREAS; -HEMORRHAGE,-PRESENT TISSUE SURROUNDING MUZZLE APPEARS SWOLLEN; ENTIRE MUZZLE COLLECTED CAUSE OF DEATH (CD) : -POSSIBLE TRAUMATIC INJURY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY, RED MATERIAL -ANIMAL OBESE -DISCHARGE-ORAL; DRY, RED MATERIAL -------------------------------------------- 1853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 104 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92536 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 808.5 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 8:30 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 91; 45 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); DORSAL; WEEK 93; 15 X 15MM; -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC -VACUOLATION, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED SURFACE OF LEFT LATERAL LOBE: SINGLE PERIPORTAL,-SLIGHT >SKIN & PELAGE; SORE/SCAB; DORSAL; DARK BROWN, SLIGHTLY DEPRESSED AREA, 8 -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED X 8 X 1 MM; COLLECTED ON ROUTINE PERIPORTAL,-MODERATE >EXCRETION; FEW FECES; VERIFIED AT SECTION -DEGENERATION / NECROSIS, NECROPSY: NOT APPLICABLE CENTRILOBULAR,-MODERATE KIDNEY (KD) : KIDNEY (KD) : -LIGHT FOCUS(I)/AREA(S); CORTEX OF -N-SARCOMA,-NEOPLASM PRESENT RIGHT KIDNEY: SINGLE TAN FOCUS, 6 MM IN DIAMETER SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -N-SARCOMA,-NEOPLASM PRESENT 1855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 106 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP TO 15 MM IN DIAMETER; RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT WITH LUNGS; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED HEART (HT) : -N-SARCOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 5 X 4 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, LOBULATED, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN ERODED AREAS, UP TO 12 X 5 MM DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC 1856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 107 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT DORSAL SHOULDER -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 15 X 13 X 5 MM, -M-FIBROSARCOMA,-NEOPLASM PRESENT TAN AND BROWN; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A DRY AND CRUSTED AREA, 15 X 13 MM; REGIONAL LN (AXILLARY) NORMAL (CAP 23) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN, TAN, AND FIRM MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT CERVICAL REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 65 X 50 X 35 MM, RED, >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS DARK RED, GRAY, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) -------------------------------------------- 1857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 108 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 1858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 109 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92538 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/14/99 13:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; OBES; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- MODERATELY SEVERE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 1859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 110 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92538 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/14/99 13:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE -INFILTRATE, LYMPHOHISTIOCYTIC,- TAN CALCULI, UP TO 6 MM IN DIAMETER MODERATELY SEVERE -HYPERPLASIA, TRANSITIONAL CELL,- MODERATELY SEVERE -MINERALIZED MATERIAL, LUMEN,-PRESENT SEMINAL VESICLES (SV) : >AUTOLYTIC CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; UP TO 30 MM IN DIAMETER -ANIMAL OBESE -------------------------------------------- 1860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 111 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92538 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/14/99 13:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 112 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-PROXIMAL; WEEK 87; EYE (EY) : VERIFIED AT NECROPSY: NOT OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >EYES; RED DISCHARGE; EYES; VERIFIED AT CONSISTS PRIMARILY OF FOCAL NECROPSY: OBSERVED MINERALIZATION,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-SEVERE KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE -M-CARCINOMA, TUBULAR CELL,-NEOPLASM PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,- MODERATE 1862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 113 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 10 X -ATROPHY/DEGENERATION,-MINIMAL 10 MM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -METAPLASIA, OSSEOUS / CARTILAGINOUS,- SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- TAN ERODED AREAS, UP TO 2 MM IN MODERATELY SEVERE DIAMETER -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING >UNREMARKABLE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 114 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : -ATROPHY,-MODERATE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE-THIRD -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE OF TAIL: MULTIPLE DARK BROWN, AND -ACANTHOSIS / HYPERKERATOSIS,-MODERATE RAISED CRUSTED AREAS; COLLECTED WITH TAIL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; EXTENDING TO MID ABDOMINAL REGION: MOIST BROWN MATERIAL -------------------------------------------- 1864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 115 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 116 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92540 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 819.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:14 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0399 .0049 % .0180 WEIGHT TAKEN RT ADRENAL (AD1) .0359 .0044 % .0162 WEIGHT TAKEN BRAIN (BR) 2.2165 .2704 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.3557 .2874 % 1.0628 WEIGHT TAKEN RT KIDNEY (KD1) 2.4085 .2938 % 1.0866 WEIGHT TAKEN LIVER (LI) 24.2163 2.9539 % 10.9255 WEIGHT TAKEN LUNG (LU) 2.7162 .3313 % 1.2254 WEIGHT TAKEN SPLEEN (SP) .8689 .1060 % .3920 WEIGHT TAKEN LF TESTIS (TE0) 1.6604 .2025 % .7491 WEIGHT TAKEN RT TESTIS (TE1) 1.5733 .1919 % .7098 WEIGHT TAKEN LF THYROID/PARA (TT0) .0148 .0018 % .0067 WEIGHT TAKEN RT THYROID/PARA (TT1) .0223 .0027 % .0101 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,- MODERATE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 1866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 117 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92540 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 819.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:14 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-SLIGHT SPINAL CORD (SC) : SPINAL CORD (SC) : >NOTE:>TRIMMING COMMENT: CERVICAL >UNREMARKABLE SPINAL CORD MISSING FROM WET >NOTE:>TWO SEGMENTS EXAMINED TISSUE THYMUS (TH) : THYMUS (TH) : >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE LUNG (LU) : -HEMORRHAGE,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE SLIGHTLY RAISED AREA, 2 -ANGIECTASIS,-PRESENT MM IN DIAMETER PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 1867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 118 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92540 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 819.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:14 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 119 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92541 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 709.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:34 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 2 X 1 -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / MM; COLLECTED ON ROUTINE SECTIONS EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 1869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 120 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92541 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 709.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:34 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 20 X 9 X 7 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE 26 X 15 X 15 MM); LEFT APPEARS SOFT >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; TISSUE SURROUNDING URETHRA -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 121 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92541 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 709.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:34 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 122 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); HEAD-MAXILLARY- LIVER (LI) : LIVER (LI) : RIGHT; WEEK 66; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES, -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED DIFFUSE: MULTIPLE DARK RED AREAS, UP -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >WART LIKE LESION(S); DORSAL-CERVICAL- TO 4 MM IN DIAMETER; COLLECTED ON EOSINOPHILIC CELL,-MINIMAL RIGHT; WEEK 69; VERIFIED AT NECROPSY: ROUTINE SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED BASOPHILIC,-MINIMAL >MASS(ES); TAIL-PROXIMAL; WEEK 93; 15 X KIDNEY (KD) : 10MM; VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); DORSAL; WEEK 103; 10 X 10MM; MODERATE VERIFIED AT NECROPSY: OBSERVED SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 1872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 123 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE >UNREMARKABLE COLON (CO) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT LATERAL -ACANTHOSIS/HYPERKERATOSIS,-MODERATELY CERVICAL REGION: SINGLE DARK RED, TAN, SEVERE AND DRY AREA, 6 X 3 X 2 MM (CAP 24) -INFLAMMATION, CHRONIC-ACTIVE,- -MASS(ES); RIGHT DORSAL THORACIC MODERATELY SEVERE REGION: SINGLE MASS, 18 X 16 X 8 MM, -CYST, EPIDERMAL INCLUSION,-PRESENT TAN AND FIRM; SAME ON CUT SURFACE; -SUPERFICIAL CRUSTING,-MODERATELY COLLECTED; REGIONAL LN (AXILLARY) SEVERE NORMAL (CAP 25) -RAISED FOCUS(I)/AREA(S); RIGHT SIDE OF MUZZLE: SINGLE DARK BROWN, TAN, AND DRY AREA, 3 X 3 X 8 MM (CAP 23) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 124 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; -HYPERPLASIA, LYMPHOPLASMACYTIC,- COLLECTED MODERATE -HEMORRHAGE,-PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); 40 MM FROM BASE: -CYST, EPIDERMAL INCLUSION,-PRESENT SINGLE TAN AREA, 12 X 7 X 3 MM; CUT SURFACE EXUDES A LIGHT GREEN SEMISOLID; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 125 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 1875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 126 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92543 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/14/00 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/14/00 7:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); NEAR DUODENUM: SINGLE MASS, -M-CARCINOMA, ISLET CELL,-NEOPLASM 4 MM IN DIAMETER, RED, TAN AND SOFT; PRESENT SAME ON CUT SURFACE (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -DIFFUSELY RED; MID ASPECT: DARK RED -B-ADENOMA,-NEOPLASM PRESENT 1876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 127 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92543 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/14/00 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/14/00 7:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT -------------------------------------------- 1877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 128 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92543 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/14/00 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/14/00 7:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 129 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT >WART LIKE LESION(S); PAW-HIND-LEFT; -NECROSIS, INDIVIDUAL HEPATOCYTE,- WEEK 95; VERIFIED AT NECROPSY: NOT MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; SWOLLEN; PAWS-HIND; BASOPHILIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >APPEARANCE; MISSING; TEETH; VERIFIED -HYPERPLASIA, BILE DUCT,-MODERATE AT NECROPSY: OBSERVED -FIBROSIS, PERIBILIARY,-SLIGHT >MASS(ES); LIMB-FRONT-LEFT; WEEK 102; KIDNEY (KD) : 30 X 30MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED SLIGHT >APPEARANCE; SWOLLEN; TAIL; VERIFIED AT PANCREAS (PA) : NECROPSY: OBSERVED -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 1879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 130 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSSIBLY >SECTION EXAMINED; TISSUE NOT PRESENT IDENTIFIED, POSSIBLE REMNANTS COLLECTED LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -MASS(ES); MUSCLE SURROUNDING LEFT -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM FRONT LEG: SINGLE MASS, 40 X 20 X 25 PRESENT MM, TAN, RED, DARK RED, GREY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 25) PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED FOCUS, 3 MM IN DIAMETER PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: UP TO 11 X 7 X 3 -HYPERPLASIA, LYMPHOPLASMACYTIC,- MM; COLLECTED MODERATELY SEVERE -LYMPHANGIECTASIS,-PRESENT 1880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 131 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : TAIL (TI) : -LARGE; ENTIRE TAIL: UP TO 15 MM IN -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT DIAMETER; COLLECTED LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 7 X 6 X 4 -LYMPHANGIECTASIS,-PRESENT MM, RIGHT IS 24 X 22 X 10 MM; BOTH ARE CYSTIC (CAP 24) JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; BILATERAL TARSAL JOINT: BOTH -INFLAMMATION, CHRONIC-ACTIVE,- ARE 13 MM IN DIAMETER; BOTH COLLECTED MODERATELY SEVERE -FIBROSIS,-SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 1881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 132 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 1882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 133 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92545 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/31/00 8:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-MODERATE CECUM (CE) : >AUTOLYTIC 1883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 134 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92545 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/31/00 8:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 135 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92545 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/31/00 8:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 136 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92546 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 317.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:03 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.0070 3.4623 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 137 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92546 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 317.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:03 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 1887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 138 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: NOT OBSERVED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; ALL LOBES -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO >BEHAVIOR; HYPOACTIVE; VERIFIED AT NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE SLIGHT >SKIN & PELAGE; YELLOW HAIR COAT; -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO VERIFIED AT NECROPSY: NOT APPLICABLE NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT SPLEEN (SP) : SPLEEN (SP) : NECROPSY: NOT APPLICABLE -LARGE; 60 X 18 X 10 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -B-ADENOMA,-NEOPLASM PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 12 X 6 -ATROPHY/DEGENERATION,-SEVERE MM; BOTH ARE SOFT 1888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 139 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE SPINAL CORD (SC) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND SOFT STOMACH, GL (ST) : >UNREMARKABLE 1889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 140 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 1 MM IN DIAMETER STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 5 X 4 MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 10 X 5 -SECRETION, DECREASED,-PRESENT MM HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 141 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 1891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 142 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92548 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 10 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC 1892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 143 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92548 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 1893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 144 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92548 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 145 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92549 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/31/99 14:51 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE CECUM (CE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 146 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92549 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/31/99 14:51 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 147 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92550 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL 1897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 148 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92550 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -EDEMA, ALVEOLAR,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -LARGE; RIGHT HINDFOOT: 45 X 14 X 12 >UNREMARKABLE MM (LEFT IS 45 X 10 X 8 MM); RIGHT APPEARS SWOLLEN; COLLECTED -------------------------------------------- 1898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 149 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92550 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 150 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SEVERE -PIGMENT,-SLIGHT 1900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 151 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 6 X 4 X 2 MM -HYPERPLASIA, C-CELL,-SLIGHT -SMALL; LEFT: 2 X 2 X 1 MM PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN -INFILTRATE, MACROPHAGE, PIGMENTED,- (CAP 23) SLIGHT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 152 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 12 X 12 X 9 MM, DARK RED, TAN AND FIRM; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 153 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 154 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED EYE (EY) : AT NECROPSY: NOT APPLICABLE -DYSTROPHY, CORNEAL IN EARLY STAGES, >APPEARANCE; THIN; VERIFIED AT CONSISTS PRIMARILY OF FOCAL NECROPSY: NOT OBSERVED MINERALIZATION,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 11 X 7 -ATROPHY/DEGENERATION,-MODERATELY MM; BOTH ARE SOFT SEVERE 1904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 155 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 MM IN DIAMETER, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY AND SOFT PROSTATE (PR) : PROSTATE (PR) : -MASS(ES); RIGHT PROXIMAL REGION, NEAR -INFLAMMATION, CHRONIC-ACTIVE,- SEMINAL VESICLE: SINGLE MASS, 12 X 10 MODERATELY SEVERE X 4 MM, GRAY, TAN AND SOFT; COLLECTED -HYPERPLASIA, EPITHELIAL,-MODERATELY INTACT WITH PROSTATE; REGIONAL LN SEVERE (LUMBAR) NORMAL (CAP 22) EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 156 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -------------------------------------------- 1906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 157 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 158 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92553 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 344.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:38 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL NOT OBSERVED SPLEEN (SP) : >APPEARANCE; MALOCCLUSION; VERIFIED AT -PIGMENT, INCREASED,-MODERATE NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >APPEARANCE; THIN; VERIFIED AT -PIGMENT,-MINIMAL NECROPSY: NOT APPLICABLE MARROW, STERNUM (SE) : >APPEARANCE; SWOLLEN; VENTRAL- -HYPERPLASIA, MYELOID, MARROW,-SLIGHT ABDOMINAL; VERIFIED AT NECROPSY: NOT LUNG (LU) : APPLICABLE -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT -INFLAMMATION, GRANULOMATOUS,-MINIMAL ESOPHAGUS (ES) : ESOPHAGUS (ES) : -LARGE; DIFFUSE: UP TO 8 MM IN -DILATATION, LUMINAL,-PRESENT DIAMETER PARATHYROID (PT) : >TISSUE MISSING PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 159 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92553 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 344.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:38 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : >NOTE:>DILATED ESOPHAGUS MAY SUGGEST OBSTRUCTION, WITH SOME ASPIRATION RESULTING IN THE GRANULOMATOUS CHANGES IN THE LUNG. GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -GI TRACT-LARGE LUMEN; DUODENUM, JEJUNUM, ILEUM AND CECUM: DUODENUM IS UP TO 10 MM IN DIAMETER; JEJUNUM, ILEUM AND CECUM ARE UP TO 20 MM IN DIAMETER; ALL ARE DISTENDED WITH GAS -------------------------------------------- 1909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 160 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92553 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 344.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:38 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 161 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92554 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 14:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -HEMORRHAGE,-MINIMAL ENTIRE; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- >SKIN & PELAGE; RED HAIR COAT; VENTRAL- MINIMAL ABDOMINAL; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -DARK FOCUS(I)/AREA(S); LEFT LATERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED LOBE: MULTIPLE DARK BROWN AREAS, UP TO -VACUOLATION, HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE 4 X 3 MM; SOME AREAS HAVE A STELLATE PERIPORTAL,-MODERATE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED PATTERN; COLLECTED ON ROUTINE SECTION -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE 1911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 162 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92554 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 14:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, -EROSION,-SLIGHT SURROUNDING PYLORIC VALVE: MULTIPLE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT BROWN AREAS, UP TO 3 X 1 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, -ACANTHOSIS / HYPERKERATOSIS,-MODERATE SURROUNDING CARDIAC VALVE: MULTIPLE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT TAN AND SLIGHTLY ERODED AREAS, UP TO 3 X 1 MM PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 5 X 4 -ATROPHY,-SEVERE MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 1912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 163 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92554 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 14:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ALL REGIONS, DIFFUSE: DRY, RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 164 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92555 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:30 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; HUNCHED; VERIFIED AT PERIPORTAL,-MODERATELY SEVERE NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYE-RIGHT; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT OBSERVED MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 1914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 165 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92555 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:30 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -------------------------------------------- 1915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 166 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92555 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:30 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 167 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92556 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 402.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:33 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, MACROPHAGE, PIGMENTED,- >APPEARANCE; THIN; VERIFIED AT MINIMAL NECROPSY: OBSERVED -GLYCOGEN, HEPATOCELLULAR, INCREASED,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MODERATELY SEVERE NECROPSY: NOT APPLICABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -HYALINE DROPLETS, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : >UNREMARKABLE >NOTE:>SEE SLIDE #17 ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 9 X 8 -ATROPHY/DEGENERATION,-MODERATE MM 1917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 168 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92556 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 402.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:33 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, VASCULAR AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 10 X 4 X 1 -SECRETION, DECREASED,-PRESENT MM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -------------------------------------------- 1918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 169 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92556 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 402.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:33 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 170 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92557 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 486.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:09 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; HUNCHED; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE SLIGHT >SKIN & PELAGE; RED HAIR COAT; VENTRAL- SPLEEN (SP) : ABDOMINAL; VERIFIED AT NECROPSY: -PIGMENT, INCREASED,-MODERATE OBSERVED PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; BILATERAL: BOTH ARE -ATROPHY/DEGENERATION,-SEVERE SOFT AND GELATINOUS -GRANULOMA, SPERM,-PRESENT -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, >NOTE:>BILATERAL BENEATH TUNICA: SINGLE YELLOW AREA, 8 X 4 MM 1920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 171 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92557 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 486.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:09 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GRAY AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 3 MM IN -ULCERATION,-SLIGHT DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: DARK RED AND MOIST -------------------------------------------- 1921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 172 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92557 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 486.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:09 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 173 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92558 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 470.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; SQUINTED; PERI-ORBITAL; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; THIN; VERIFIED AT MINIMAL NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT 1923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 174 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92558 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 470.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL TAN ERODED AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN GENERAL INFORMATION (XX) : >NOTE:>IDENTIFICATION TRANSPONDER FOUND WITHIN ADIPOSE TISSUE OF ABDOMINAL CAVITY -------------------------------------------- 1924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 175 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92558 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 470.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 176 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92559 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/22/99 15:34 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-PROXIMAL; WEEK 72; 25 X LIVER (LI) : LIVER (LI) : 20MM; VERIFIED AT NECROPSY: NOT -LARGE; ALL LOBES: THICKENED -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO OBSERVED -MOTTLED; ALL LOBES: RED, DARK RED AND NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >SKIN & PELAGE; SORE/SCAB; TAIL- TAN PROXIMAL; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : OBSERVED -HYALINE DROPLETS, TUBULAR EPITHELIUM,- >MASS(ES); VENTRAL-THORACIC; WEEK 79; MODERATE 20 X 20MM; VERIFIED AT NECROPSY: NOT SPLEEN (SP) : OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 177 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92559 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/22/99 15:34 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: DARK RED AND TAN -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT SACRAL REGION: SINGLE -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT MASS, 25 MM IN DIAMETER; DARK RED, TAN -INFLAMMATION, CHRONIC-ACTIVE,- AND FIRM; SAME ON CUT SURFACE; MODERATELY SEVERE COLLECTED; REGIONAL LN (INGUINAL) NOT -BACTERIAL COLONIES,-PRESENT IDENTIFIED -SUPERFICIAL CRUSTING,-MODERATELY SEVERE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT 1927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 178 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92559 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/22/99 15:34 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, OTHER (LN) : LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 10 X 6 X 6 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO (CAP 22) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 1928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 179 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92560 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 10/31/99 14:18 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-PROXIMAL; WEEK 79; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >SKIN & PELAGE; YELLOW HAIR COAT; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 1929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 180 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92560 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 10/31/99 14:18 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 4 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); NEAR BASE OF TAIL: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SINGLE SOFT AND RAISED AREA, 15 X 10 X -CYST, EPIDERMAL INCLUSION,-PRESENT 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 1930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 181 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92560 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 10/31/99 14:18 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TROY ELLENBECKER ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 182 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92561 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 436 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 06/29/99 14:35 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >RESPIRATION; AUDIBLE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -NECROSIS, INDIVIDUAL HEPATOCYTE,- NECROPSY: NOT APPLICABLE MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -PIGMENT, TUBULAR,-MINIMAL -HYALINE DROPLETS, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 1932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 183 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92561 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 436 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 06/29/99 14:35 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT -ANGIECTASIS,-PRESENT RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 184 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92561 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 436 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 06/29/99 14:35 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 185 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92562 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 667.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0217 .0032 % .0094 WEIGHT TAKEN RT ADRENAL (AD1) .0222 .0033 % .0096 WEIGHT TAKEN BRAIN (BR) 2.3159 .3468 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.1698 .3250 % .9369 WEIGHT TAKEN RT KIDNEY (KD1) 2.1881 .3277 % .9448 WEIGHT TAKEN LIVER (LI) 19.3134 2.8925 % 8.3395 WEIGHT TAKEN LUNG (LU) 1.9553 .2928 % .8443 WEIGHT TAKEN SPLEEN (SP) .9195 .1377 % .3970 WEIGHT TAKEN LF TESTIS (TE0) 1.6813 .2518 % .7260 WEIGHT TAKEN RT TESTIS (TE1) 1.6647 .2493 % .7188 WEIGHT TAKEN LF THYROID/PARA (TT0) .0204 .0031 % .0088 WEIGHT TAKEN RT THYROID/PARA (TT1) .0190 .0028 % .0082 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 1935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 186 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92562 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 667.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 187 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92562 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 667.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 188 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92563 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 189 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92563 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED, TAN AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: -GALACTOCELE,-PRESENT MULTIPLE TAN AND CREAMY SEMIFLUID -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 190 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92563 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 191 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92564 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/19/99 STUDY DAY OF DEATH: 487 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 681.9 GRAMS DATE AND TIME OF NECROPSY: 08/19/99 7:55 PROSECTOR: TONY HANFELD RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NONFORMED FECES; VERIFIED BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -M-ASTROCYTOMA,-NEOPLASM PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-SLIGHT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; THIN; VERIFIED AT MODERATE NECROPSY: NOT OBSERVED SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 5 X 2 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT 1941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 192 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92564 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/19/99 STUDY DAY OF DEATH: 487 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 681.9 GRAMS DATE AND TIME OF NECROPSY: 08/19/99 7:55 PROSECTOR: TONY HANFELD RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -RAISED AREA(S); RIGHT POSTERIOR ASPECT: SINGLE TAN AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, WITHIN -ACANTHOSIS / HYPERKERATOSIS,-MODERATE THICKENED AREA: MULTIPLE DARK BROWN -ULCERATION,-MINIMAL ERODED AREAS, UP TO 8 X 2 MM -INFLAMMATION, CHRONIC-ACTIVE,- -THICKENED WALL; NEAR ESOPHAGEAL MODERATELY SEVERE GROOVE: SINGLE AREA, 15 X 9 MM CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 193 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92564 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/19/99 STUDY DAY OF DEATH: 487 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 681.9 GRAMS DATE AND TIME OF NECROPSY: 08/19/99 7:55 PROSECTOR: TONY HANFELD RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 194 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >RESPIRATION; LABORED; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE,-SLIGHT >DISCHARGE; NASAL; RED IN COLOR; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; LIMITED USE; PAWS-HIND; PERIPORTAL,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 1944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 195 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATELY SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL -INFLAMMATION, SUBACUTE,-MINIMAL DUODENUM (DU) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -EROSION,-MINIMAL ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 196 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -DISCHARGE-NASAL; DARK RED AND MOIST -ANIMAL OBESE -------------------------------------------- 1946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 197 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 198 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92566 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 155 STUDY WEEK OF DEATH: 23 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 10:53 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -M-OLIGODENDROGLIOMA,-NEOPLASM PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT LUNG (LU) : -CONGESTION,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: MISSING 1948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 199 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92566 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 155 STUDY WEEK OF DEATH: 23 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 10:53 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -------------------------------------------- 1949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 200 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92566 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 155 STUDY WEEK OF DEATH: 23 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 10:53 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 201 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE 1951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 202 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -MASS(ES); LEFT BICEPS FEMORIS: SINGLE -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM MASS, 40 X 20 X 20 MM, DARK RED, TAN, PRESENT LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (POPLITEAL) NOT IDENTIFIED NERVE, SCIATIC (SN) : >NOTE:>RIGHT SCIATIC NERVE COLLECTED DUE TO SM MASS ON LEFT THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 9 X 7 X 7 MM (LEFT IS 8 >UNREMARKABLE X 5 X 5 MM) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC 1952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 203 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 1953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 204 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 205 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92568 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/20/00 STUDY DAY OF DEATH: 701 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/20/00 13:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 95; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; DORSAL; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: OBSERVED EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); RIGHT ON CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- SINGLE CLEAR FLUID FILLED CYST, 1 MM SEVERE IN DIAMETER -DILATATION, PELVIC,-SLIGHT -LARGE; BILATERAL: EACH IS 30 X 20 X -MINERALIZATION, PELVIC,-MINIMAL 12 MM SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT:9 X 6 X 6 MM (RIGHT IS 5 -B-ADENOMA,-NEOPLASM PRESENT X 4 X 2 MM) -MOTTLED; BILATERAL: RED, DARK RED AND TAN ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 1955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 206 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92568 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/20/00 STUDY DAY OF DEATH: 701 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/20/00 13:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -INFLAMMATION, VASCULAR,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM REGION: SINGLE DARK RED, DRY SLIGHTLY PRESENT RAISED AREA, 10 X 6 X 4 MM (CAP 22) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFLAMMATION, VASCULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT -------------------------------------------- 1956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 207 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92568 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/20/00 STUDY DAY OF DEATH: 701 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/20/00 13:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 208 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK EYE (EY) : 93; 10 X 10MM; VERIFIED AT NECROPSY: -DYSTROPHY, CORNEAL IN EARLY STAGES, OBSERVED CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL 1958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 209 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE MASS, 20 X 15 X 10 MM, RED, MODERATELY SEVERE TAN, GREY AND FIRM; SAME ON CUT -M-CARCINOMA,- 2 NEOPLASMS PRESENT SURFACE; (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT AXILLARY REGION, CRANIAL TO MM MASS #1: SINGLE MASS, 25 X 15 X 12 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (AXILLARY) NORMAL (CAP 23) 1959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 210 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, -INFLAMMATION / NECROSIS, FAT,- NEAR STOMACH: SINGLE MASS, 20 X 12 X MODERATELY SEVERE 10 MM, YELLOW, TAN AND FIRM; (CAP 25) -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE REGIONAL LN (PANCREATIC) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 1960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 211 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 1961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 212 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92570 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 648.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0288 .0044 % .0119 WEIGHT TAKEN RT ADRENAL (AD1) .0271 .0042 % .0112 WEIGHT TAKEN BRAIN (BR) 2.4190 .3733 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.6246 .4050 % 1.0850 WEIGHT TAKEN RT KIDNEY (KD1) 2.5830 .3986 % 1.0678 WEIGHT TAKEN LIVER (LI) 17.2061 2.6553 % 7.1129 WEIGHT TAKEN LUNG (LU) 2.5087 .3871 % 1.0371 WEIGHT TAKEN SPLEEN (SP) .9586 .1479 % .3963 WEIGHT TAKEN LF TESTIS (TE0) 1.9006 .2933 % .7857 WEIGHT TAKEN RT TESTIS (TE1) 1.9018 .2935 % .7862 WEIGHT TAKEN LF THYROID/PARA (TT0) .0274 .0042 % .0113 WEIGHT TAKEN RT THYROID/PARA (TT1) .0279 .0043 % .0115 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MODERATE 1962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 213 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92570 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 648.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 1963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 214 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92570 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 648.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 215 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92571 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 645.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 12:32 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT >BEHAVIOR; ATAXIC; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-MODERATE >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: NOT APPLICABLE BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 1965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 216 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92571 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 645.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 12:32 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT -HYPERPLASIA, MEDULLARY,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 20 X 12 X 3 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE 23 X 14 X 8 MM); LEFT IS SOFT >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT URINARY BLADDER (UB) : URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; RED SEMIFLUID -HEMORRHAGE,-MINIMAL -THICKENED WALL; DIFFUSE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 1966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 217 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92571 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 645.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 12:32 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 218 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -DYSTROPHY, CORNEAL IN EARLY STAGES, >APPEARANCE; THIN; VERIFIED AT CONSISTS PRIMARILY OF FOCAL NECROPSY: NOT OBSERVED MINERALIZATION,-MINIMAL BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -MOTTLED; BILATERAL: RED AND TAN SEVERE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATELY SEVERE 1968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 219 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATE TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-MODERATE -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,- SLIGHT -MINERALIZATION, VASCULAR,-SLIGHT CECUM (CE) : >AUTOLYTIC 1969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 220 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; BROWN MATERIAL -------------------------------------------- 1970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 221 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 222 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92573 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 530.3 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:41 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MISSING; TEETH; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-SLIGHT NECROPSY: NOT OBSERVED -GLYCOGEN, HEPATOCELLULAR, INCREASED,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MODERATELY SEVERE NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED SLIGHT >EXCRETION; FEW FECES; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT >NOTE:>UNILATERAL 1972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 223 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92573 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 530.3 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:41 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT -DEPLETION, LYMPHOCYTIC (GENERALLY POSITIVELY IDENTIFIED; POSSIBLE COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- REMNANTS COLLECTED SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 1973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 224 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92573 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 530.3 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:41 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 225 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92574 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.0928 3.6630 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 226 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92574 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 1976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 227 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92575 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 288.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:32 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.4038 3.6115 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 1977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 228 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92575 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 288.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:32 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 1978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 229 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92576 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 792.6 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:34 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 73; 30 X 40MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; DORSAL; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; LIMITED USE; LIMBS-HIND; -VACUOLATION, HEPATOCELLULAR, MIDZONAL VERIFIED AT NECROPSY: NOT APPLICABLE / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT BONE, FEMUR (FE) : -HYPERPLASIA, BONE,-SLIGHT 1979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 230 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92576 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 792.6 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:34 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF -CARDIOMYOPATHY,-SLIGHT BOTH VENTRICLES: MULTIPLE TAN, INDISTINCT AREAS THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND SOFT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC -INFLAMMATION, CHRONIC-ACTIVE,- REGION: SINGLE BLACK CRUSTED AREA, 30 MODERATELY SEVERE X 20 MM; COLLECTED; POSSIBLY -ULCERATION,-SLIGHT CONSISTENT WITH MASS NOTED IN CLINICAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT OBSERVATIONS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 1980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 231 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92576 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 792.6 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:34 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 232 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92577 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 895.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 69; 30 X 30MM; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; DORSAL; -FOCUS, ALTERED HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED BASOPHILIC,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK KIDNEY (KD) : 101; 30 X 40MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MODERATELY SEVERE >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK -CYST,-PRESENT 105; VERIFIED AT NECROPSY: OBSERVED PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); ATTACHED TO LOWER LEFT LIP: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE MASS, 15 X 13 X 8 MM, RED, DARK MODERATELY SEVERE RED, GRAY, AND SOFT; ON SECTION EXUDES -B-KERATOACANTHOMA,-NEOPLASM PRESENT A DARK BROWN GELATINOUS FLUID (CAP -B-FIBROMA,-NEOPLASM PRESENT 22); REGIONAL LN (MANDIBULAR) NORMAL -FIBROSIS,-MODERATE (CAP 23) >NOTE:>MASS #2 IS THE KERATOACANTHOMA THE FIBROMA WAS DESCRIBED GROSSLY UNDER MAMMARY, MALE 1982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 233 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92577 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 895.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -MASS #2; RIGHT LUMBAR REGION: SINGLE MASS, 32 X 20 X 19 MM, RED, GRAY, TAN, CAULIFLOWER-LIKE, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 26); OVERLYING SKIN HAS A SINGLE DRY, TAN CRUSTED AREA, 10 X 10 X 5 MM; COLLECTED INTACT WITH MASS URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 53 X 45 X 18 MM, RED, >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS GRAY, TAN, AND SOFT; SAME ON CUT THIS MASS IS THE FIBROMA SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE 1983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 234 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92577 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 895.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 1984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 235 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92578 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 707.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT LOBE, AT JUNCTION OF LEFT MEDIAN LOBE: -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE SINGLE TAN AREA, 4 MM IN DIAMETER; PERIPORTAL,-SLIGHT >SKIN & PELAGE; PALE; ENTIRE BODY; COLLECTED ON ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); LEFT LATERAL EOSINOPHILIC CELL,-SLIGHT >EYES; RED DISCHARGE; EYE-LEFT; LOBE: MULTIPLE DARK RED AREAS, UP TO 3 -DEGENERATION, CYSTIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL 1985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 236 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92578 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 707.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 6 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, SOFT AND LOBULATED HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY DARK RED MATERIAL -------------------------------------------- 1986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 237 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92578 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 707.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 238 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; PALE; ENTIRE BODY; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT SURFACE OF LEFT LATERAL LOBE, NEAR PERIPORTAL,-MODERATE NECROPSY: OBSERVED MARGIN: SINGLE TAN AREA, 5 X 4 MM; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >MASS(ES); VENTRAL-ABDOMINAL; WEEK 86; COLLECTED ON ROUTINE SECTION EOSINOPHILIC CELL,-MINIMAL VERIFIED AT NECROPSY: NOT OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN -DEGENERATION, CYSTIC,-MINIMAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE NECROPSY: NOT APPLICABLE SPLEEN (SP) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE INCREASED,-MINIMAL >RESPIRATION; LABORED; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-SLIGHT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MODERATE 1988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 239 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : HEART (HT) : -DIFFUSELY LIGHT; TAN -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 MM IN -HYPERPLASIA,-SEVERE DIAMETER PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE -MINERALIZATION, GLANDULAR MUCOSA,- MODERATE CECUM (CE) : CECUM (CE) : -DIFFUSELY RED; MUCOSA -EROSION,-MODERATE 1989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 240 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : CECUM (CE) : -THICKENED WALL; DIFFUSE -HEMORRHAGE,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); MID -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT VENTRAL ABDOMINAL REGION: SINGLE MOIST -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE TAN ULCERATED AREA, 5 MM IN DIAMETER -ULCERATION,-MODERATE (CAP 22) -FIBROSIS,-MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; RIGHT: DARK RED (CAP -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 23) -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- 1990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 241 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 242 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92580 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 503.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 11:30 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NONFORMED FECES; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >EYES; RED DISCHARGE; EYE-LEFT; PERIPORTAL,-MODERATELY SEVERE VERIFIED AT NECROPSY: OBSERVED -NECROSIS, COAGULATIVE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 1992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 243 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92580 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 503.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 11:30 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 MM IN DIAMETER, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM PROSTATE (PR) : PROSTATE (PR) : -SMALL; 15 X 10 X 5 MM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 5 X 2 -SECRETION, DECREASED,-PRESENT MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY AND RED MATERIAL -------------------------------------------- 1993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 244 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92580 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 503.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 11:30 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 245 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); LIMB-HIND-RIGHT; WEEK 93; 10 BRAIN (BR) : BRAIN (BR) : X 10MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >WART LIKE LESION(S); DORSAL; WEEK 95; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT PERIPORTAL,-SLIGHT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >SKIN & PELAGE; RED HAIR COAT; HEAD- -NECROSIS, COAGULATIVE,-SLIGHT ENTIRE; VERIFIED AT NECROPSY: NOT -INFILTRATE, MACROPHAGE, PIGMENTED,- OBSERVED MINIMAL >EXCRETION; NO FECES; VERIFIED AT -KARYOMEGALY,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >APPEARANCE; HUNCHED; VERIFIED AT -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT 1995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 246 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL 1996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 247 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT TAN ERODED AREAS, UP TO 3 MM IN DIAMETER SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT LATERAL -CYST, EPIDERMAL INCLUSION,-PRESENT THORACIC REGION: SINGLE TAN AND DRY -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM AREA, 8 X 5 MM (CAP 24); POSSIBLY PRESENT CONSISTENT WITH WART-LIKE LESION >NOTE:>PAPILLOMA IS ON SLIDE #24 BOTH (DORSAL) AS NOTED IN CLINICAL MASSES ARE INCLUSION CYSTS OBSERVATIONS -MASS(ES); RIGHT THIGH: SINGLE MASS, 17 X 12 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (POPLITEAL) NOT IDENTIFIED; OVERLYING SKIN HAS TWO TAN AND DRY ABRADED AREAS, BOTH ARE 3 MM IN DIAMETER; COLLECTED INTACT WITH MASS -MASS #2; RIGHT FLANK: SINGLE MASS, 10 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED PROSTATE (PR) : PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: BROWN -INFLAMMATION, CHRONIC-ACTIVE,- AND FIRM MODERATELY SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 6 X 2 -ATROPHY,-SEVERE MM 1997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 248 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 1998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 249 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); SHOULDER-LEFT; WEEK 98; 60 X LIVER (LI) : LIVER (LI) : 40MM; VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >SKIN & PELAGE; SORE/SCAB; SHOULDER- MULTIPLE TAN, FIRM AREAS, UP TO 9 X 5 EOSINOPHILIC CELL,-MINIMAL LEFT; VERIFIED AT NECROPSY: OBSERVED MM; COLLECTED ON ROUTINE SECTIONS; -DEGENERATION, CYSTIC,-SLIGHT >EXCRETION; FEW FECES; VERIFIED AT EXTRA SECTIONS (CAP 22) -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: NOT APPLICABLE NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >APPEARANCE; LIMITED USE; LIMB-FRONT- KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: NOT -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYALINE DROPLETS, TUBULAR EPITHELIUM,- NECROPSY: NOT APPLICABLE MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-MODERATE -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,- MODERATE 1999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 250 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE TAN, FIRM AREAS, UP TO 4 X 2 NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MM HEART (HT) : -CARDIOMYOPATHY,-MINIMAL ESOPHAGUS (ES) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 4 MM IN -ULCERATION,-MODERATE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 251 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION >NOT REQUIRED TO BE EXAMINED FOR ANIMAL EXTENDS TO LEFT SHOULDER REGION: >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS SINGLE MASS, 45 X 25 X 15 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A BROWN, RED, DRY CRUSTED AREA, 30 X 25 MM; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 252 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 253 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92583 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 74; 15 X 15MM; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; DORSAL; SURFACE OF RIGHT MEDIAN LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: OBSERVED TAN AREA, 3 MM IN DIAMETER; COLLECTED EOSINOPHILIC CELL,-MODERATE ON ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -LARGE; BILATERAL: EACH IS 30 X 20 X SEVERE 12 MM -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 6 MM (RIGHT IS 5 -CORTICAL TISSUE, EXTRACAPSULAR,- X 4 X 3 MM) PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE 2003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 254 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92583 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -INFLAMMATION, VASCULAR,-SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 X 5 X 3 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE SKIN (SK) : SKIN (SK) : -MASS(ES); MID DORSAL LUMBAR REGION: -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM SINGLE MASS, 15 X 15 X 10 MM, TAN AND PRESENT FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE BROWN ULCERATED AREA, 7 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 25) URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: 10 X 6 X 3 MM (RIGHT IS -HEMORRHAGE,-PRESENT 5 X 4 X 3 MM) -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT 2004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 255 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92583 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : -CYST(S); BILATERAL, PECTORAL REGIONS: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 15 X 12 X 5 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 256 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92584 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 418 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 401.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 13:56 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 2006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 257 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92584 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 418 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 401.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 13:56 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -------------------------------------------- 2007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 258 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92584 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 418 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 401.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 13:56 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 259 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -CONGESTION,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO AT NECROPSY: OBSERVED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >RESPIRATION; LABORED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -REGENERATION, TUBULAR EPITHELIUM,- >MASS(ES); VENTRAL-CERVICAL; WEEK 37; MINIMAL 30 X 20MM; VERIFIED AT NECROPSY: SPLEEN (SP) : SPLEEN (SP) : OBSERVED -LARGE; 50 X 15 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, >MASS(ES); AXILLARY REGION-RIGHT; WEEK INCREASED,-MODERATE 37; 20 X 20MM; VERIFIED AT NECROPSY: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO OBSERVED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >EXCRETION; FEW FECES; VERIFIED AT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : NECROPSY: NOT APPLICABLE -LARGE; UP TO 10 X 10 X 8 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO >MASS(ES); AXILLARY REGION-LEFT; WEEK NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 39; 10 X 10MM; VERIFIED AT NECROPSY: MARROW, FEMUR (FM) : OBSERVED -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 260 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 35 X 35 X 25 MM, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ILEUM (IL) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 9 X 1 -SECRETION, DECREASED,-PRESENT MM HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 20 X 20 X 15 MM (CAP 23) -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 261 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 40 X -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 22 X 15 MM (CAP 22); POSSIBLY NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CONSISTENT WITH MASSES (LEFT AND RIGHT AXILLARY REGIONS) AS NOTED IN CLINICAL OBSERVATIONS LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; BILATERAL: EACH IS 11 X 8 X 6 -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MM (CAP 24) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, OTHER (LN) : LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 25 X 15 X 15 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO (CAP 25) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS 25 X 17 X -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 15 MM (CAP 26) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 25 X -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 15 X 12 MM; COLLECTED; POSSIBLY NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CONSISTENT WITH MASS (VENTRAL CERVICAL) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF RED FLUID 2011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 262 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 263 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92586 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 518.1 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:10 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; YELLOW HAIR COAT; PERIPORTAL,-MODERATE VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM 2013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 264 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92586 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 518.1 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:10 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- TAN ERODED AREAS, UP TO 2 MM IN MODERATELY SEVERE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -THICKENED WALL; DIFFUSE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -THICKENED; BILATERAL: RED, GRAY AND -HYPERPLASIA, EPITHELIAL,-SLIGHT FIRM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 265 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92586 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 518.1 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:10 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 266 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92587 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 480.5 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; RED HAIR COAT; HEAD- PERIPORTAL,-SLIGHT ENTIRE; VERIFIED AT NECROPSY: NOT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -ROUGH SURFACE, DIFFUSE; BILATERAL SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE 2016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 267 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92587 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 480.5 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 10 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 2 MM IN DIAMETER URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: -GALACTOCELE,-PRESENT MULTIPLE TAN AND CREAMY SEMIFLUID -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) 2017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 268 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92587 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 480.5 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -DISCHARGE-ORAL; RED AND MOIST -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 269 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT BASOPHILIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL 2019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 270 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL NERVE, SCIATIC (SN) : -DEGENERATION,-MINIMAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, -B-ADENOMA,-NEOPLASM PRESENT DARK RED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : PROSTATE (PR) : -LARGE; 35 X 20 X 10 MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 40 X 20 X 5 -SECRETION, INCREASED,-PRESENT MM 2020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 271 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -THICKENED; BILATERAL, AXILLARY -GALACTOCELE,-PRESENT REGIONS: TAN AND FIRM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING -------------------------------------------- 2021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 272 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 273 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92589 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 394.9 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:21 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; HYPOACTIVE; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >BEHAVIOR; ATAXIC; VERIFIED AT BASOPHILIC,-MINIMAL NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -ANGIECTASIS,-SLIGHT FILLED CYST, 2 MM IN DIAMETER -PIGMENT,-SLIGHT 2023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 274 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92589 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 394.9 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:21 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 2024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 275 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92589 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 394.9 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:21 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 276 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92590 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 834.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:55 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 8 X 6 X 5 -ANGIECTASIS,-MODERATELY SEVERE MM TESTIS (TE) : TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); LEFT, -MINERALIZATION, VASCULAR,-MINIMAL BENEATH TUNICA: DIFFUSE -B-INTERSTITIAL CELL TUMOR,-NEOPLASM -LARGE; LEFT: 40 X 22 X 17 MM (RIGHT PRESENT IS 22 X 14 X 12 MM) PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HYPERPLASIA,-MODERATE MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM 2026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 277 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92590 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 834.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:55 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE -EDEMA,-MODERATELY SEVERE -INFLAMMATION, ACUTE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 278 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92591 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/18/00 7:16 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 87; VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES: MARGINS APPEAR -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK ROUNDED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 95; 10 X 10MM; VERIFIED AT NECROPSY: EOSINOPHILIC CELL,-MINIMAL OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; LEFT: CLEAR FLUID -ATROPHY/DEGENERATION,-MODERATELY SEVERE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 2028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 279 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92591 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/18/00 7:16 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : >AUTOLYTIC SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE TAN CREAMY SEMIFLUID FILLED >NOTE:>SEE SKIN FOR DIAGNOSIS CYSTS, UP TO 16 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION; POSSIBLY CONSISTENT WITH MASSES NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -DISCHARGE-ORAL; YELLOW AND DRY -------------------------------------------- 2029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 280 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92591 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/18/00 7:16 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 281 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92592 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; LIMBS-HIND; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -PIGMENT,-MINIMAL SPINAL CORD (SC) : -NECROSIS / HEMORRHAGE, WHITE MATTER,- MODERATELY SEVERE 2031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 282 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92592 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -DEGENERATION/NECROSIS/HEMORRHAGE, SPINAL CORD/BRAIN,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -------------------------------------------- 2032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 283 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92592 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 284 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92593 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 479.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0279 .0058 % .0121 WEIGHT TAKEN RT ADRENAL (AD1) .0154 .0032 % .0067 WEIGHT TAKEN BRAIN (BR) 2.3137 .4822 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.0761 .4327 % .8973 WEIGHT TAKEN RT KIDNEY (KD1) 1.9731 .4112 % .8528 WEIGHT TAKEN LIVER (LI) 15.4451 3.2191 % 6.6755 WEIGHT TAKEN LUNG (LU) 1.6009 .3337 % .6919 WEIGHT TAKEN SPLEEN (SP) .7002 .1459 % .3026 WEIGHT TAKEN LF TESTIS (TE0) 1.6486 .3436 % .7125 WEIGHT TAKEN RT TESTIS (TE1) 1.6414 .3421 % .7094 WEIGHT TAKEN LF THYROID/PARA (TT0) .0144 .0030 % .0062 WEIGHT TAKEN RT THYROID/PARA (TT1) .0110 .0023 % .0048 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 285 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92593 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 479.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED >NOT REQUIRED TO BE EXAMINED FOR ANIMAL (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 286 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92594 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 13:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES: MARGINS APPEAR -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ROUNDED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : -HYPERPLASIA,-SEVERE 2036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 287 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92594 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 13:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -RAISED AREA(S); LEFT ASPECT: SINGLE TAN, SLIGHTLY RAISED FOCUS, 2 MM IN DIAMETER ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT -------------------------------------------- 2037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 288 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92594 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 13:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 289 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL- BRAIN (BR) : BRAIN (BR) : THORACIC; VERIFIED AT NECROPSY: -MASS(ES); WITHIN CEREBRUM: SINGLE -M-GRANULAR CELL TUMOR,-NEOPLASM OBSERVED MASS, 12 X 8 X 8 MM, TAN AND SOFT; PRESENT >MASS(ES); DORSAL; WEEK 73; 25 X 30MM; COLLECTED INTACT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- >EYES; RED DISCHARGE; EYES; VERIFIED AT MINIMAL NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >SKIN & PELAGE; PALE; ENTIRE BODY; EOSINOPHILIC CELL,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT >APPEARANCE; THIN; VERIFIED AT -FIBROSIS, PERIBILIARY,-MINIMAL NECROPSY: NOT OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); WITHIN PANCREAS: SINGLE -ATROPHY, ACINAR,-SLIGHT MASS, 10 X 10 X 8 MM, DARK RED, TAN -M-CARCINOMA, ISLET CELL,-NEOPLASM AND FIRM; SAME ON CUT SURFACE (CAP PRESENT 22); REGIONAL LN (SEE PANCREATIC) ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -VACUOLATION, DIFFUSE,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL 2039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 290 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); MID DORSAL THORACIC REGION: -B-ADENOMA, BASAL SINGLE MASS, 30 X 30 X 12 MM, DARK CELL/SQUAMOUS/SEBACEOUS,-NEOPLASM RED, TAN AND FIRM; SAME ON CUT PRESENT SURFACE; COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED AND TAN MOIST ULCERATED AREA, 20 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 24) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 7 X 2 -ATROPHY,-SEVERE MM 2040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 291 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -MOTTLED; PANCREATIC: RED AND TAN (CAP -HEMORRHAGE,-PRESENT 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 2041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 292 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 293 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92596 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 473.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:58 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >SKIN & PELAGE; BROWN HAIR COAT; EOSINOPHILIC CELL,-SLIGHT SCROTUM; VERIFIED AT NECROPSY: NOT -GLYCOGEN, HEPATOCELLULAR, INCREASED,- OBSERVED MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 294 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92596 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 473.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:58 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 9 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92596 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 473.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:58 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 296 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92597 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 670.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LEFT; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL TESTIS (TE) : TESTIS (TE) : -LARGE; RIGHT: 25 X 17 X 10 MM (LEFT -ATROPHY/DEGENERATION,-SEVERE IS 20 X 13 X 9 MM); BOTH ARE SOFT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE HINDFOOT: SINGLE DARK RED, MOIST -ULCERATION,-MODERATE ABRADED AREA, 8 MM IN DIAMETER; -ACANTHOSIS / HYPERKERATOSIS,-MODERATE SURROUNDING TISSUE APPEARS SWOLLEN; -BACTERIA,-PRESENT COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 297 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92597 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 670.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 298 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92598 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 749.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT 2048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 299 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92598 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 749.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 300 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92599 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 8:39 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: BROWN -NECROSIS, COAGULATIVE,-SLIGHT -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, SUBACUTE,-SLIGHT MULTIPLE TAN, SLIGHTLY INDISTINCT AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; LEFT: CLEAR FLUID; -ATROPHY/DEGENERATION,-SEVERE LEFT IS ALSO SOFT >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT URINARY BLADDER (UB) : >UNREMARKABLE 2050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 301 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92599 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 8:39 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; DARK BROWN SEMIFLUID PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 2051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 302 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92599 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 8:39 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 303 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92600 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0349 .0074 % .0156 WEIGHT TAKEN RT ADRENAL (AD1) .0286 .0061 % .0128 WEIGHT TAKEN BRAIN (BR) 2.2319 .4731 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.7574 .3725 % .7874 WEIGHT TAKEN RT KIDNEY (KD1) 1.7944 .3803 % .8040 WEIGHT TAKEN LIVER (LI) 15.0729 3.1948 % 6.7534 WEIGHT TAKEN LUNG (LU) 1.7674 .3746 % .7919 WEIGHT TAKEN SPLEEN (SP) .7466 .1582 % .3345 WEIGHT TAKEN LF TESTIS (TE0) 1.6879 .3578 % .7563 WEIGHT TAKEN RT TESTIS (TE1) 1.6725 .3545 % .7494 WEIGHT TAKEN LF THYROID/PARA (TT0) .0093 .0020 % .0042 WEIGHT TAKEN RT THYROID/PARA (TT1) .0112 .0024 % .0050 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) >NOT REQUIRED TO BE EXAMINED FOR ANIMAL GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 304 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92600 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 305 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92601 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 827.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 104; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); LEFT ANTERIOR -HYPERPLASIA,-MODERATE ASPECT: SINGLE TAN AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 306 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92601 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 827.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL SHOULDER REGION: SINGLE TAN, SLIGHTLY RAISED, CRUSTED AREA, 8 MM IN DIAMETER (CAP 22) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 307 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92601 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 827.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 308 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92602 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 20 X 13 X 3 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE 22 X 16 X 9 MM) -CONTAINS FLUID; LEFT BENEATH TUNICA: CLEAR FLUID THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 309 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92602 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 310 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92603 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/98 STUDY DAY OF DEATH: 127 STUDY WEEK OF DEATH: 19 TERMINAL BODY WEIGHT: 521.4 GRAMS DATE AND TIME OF NECROPSY: 08/24/98 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JOHN S. HALFORD ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; PARALYSIS; LIMBS-HIND; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >BEHAVIOR; COLD TO TOUCH; LIMBS-HIND; CENTRILOBULAR POLYKARYOCYTOSIS VERIFIED AT NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SPINAL CORD (SC) : SPINAL CORD (SC) : -FRACTURE(S); BETWEEN L3 AND L5: >UNREMARKABLE SINGLE INDISTINCT FRACTURE; COLLECTED >NOTE:>SEE VERTEBRA 2060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 311 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92603 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/98 STUDY DAY OF DEATH: 127 STUDY WEEK OF DEATH: 19 TERMINAL BODY WEIGHT: 521.4 GRAMS DATE AND TIME OF NECROPSY: 08/24/98 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JOHN S. HALFORD ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT VERTEBRA/MARROW (VT) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),- NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 312 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92603 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/98 STUDY DAY OF DEATH: 127 STUDY WEEK OF DEATH: 19 TERMINAL BODY WEIGHT: 521.4 GRAMS DATE AND TIME OF NECROPSY: 08/24/98 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JOHN S. HALFORD ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 313 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92604 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 405.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; BROWN HAIR COAT; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VENTRAL-ABDOMINAL; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT >UNREMARKABLE IS 4 X 3 X 3 MM) ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE 2063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 314 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92604 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 405.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, LOBULATED AND FIRM HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 34 X 17 X 8 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -------------------------------------------- 2064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 315 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92604 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 405.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 316 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 87; 40 X 50MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >MASS(ES); DORSAL; WEEK 79; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MODERATE >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -HYPERPLASIA, BILE DUCT,-SLIGHT NOT OBSERVED -FIBROSIS, PERIBILIARY,-SLIGHT >SKIN & PELAGE; SORE/SCAB; DORSAL; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; RED HAIR COAT; HEAD- SEVERE ENTIRE; VERIFIED AT NECROPSY: OBSERVED SPLEEN (SP) : >RESPIRATION; AUDIBLE; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE INCREASED,-MODERATELY SEVERE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: NOT APPLICABLE -LARGE; LEFT: 9 MM IN DIAMETER (RIGHT -VACUOLATION, FOCAL,-SLIGHT IS 7 X 4 X 3 MM) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATELY SEVERE TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL 2066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 317 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MODERATE BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -MINERALIZATION, VASCULAR,-MODERATE -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATELY SEVERE -MINERALIZATION,-MODERATE NERVE, SCIATIC (SN) : >TISSUE MISSING THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : -HYPERPLASIA,-SLIGHT 2067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 318 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE -MINERALIZATION, GLANDULAR MUCOSA,- MODERATE JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT DORSAL LUMBAR REGION: -B-KERATOACANTHOMA,-NEOPLASM PRESENT SINGLE MASS, 15 MM IN DIAMETER, TAN, -B-LIPOMA,-NEOPLASM PRESENT FIRM AND FRIABLE; SAME ON CUT SURFACE; >NOTE:>LIPOMA IS MASS DESCRIBED UNDER COLLECTED; REGIONAL LN (INGUINAL) NOT MAMMARY, MALE IDENTIFIED PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT ABDOMINAL REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 45 X 35 X 25 MM, TAN AND >NOTE:>SEE SKIN FOR DIAGNOSIS FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL, COVERING ENTIRE HEAD: DRY RED MATERIAL 2068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 319 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -TRACHEA-CONTENT FOAMY MATTER; WHITE FOAMY MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 320 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92606 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/13/99 STUDY DAY OF DEATH: 542 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 669.7 GRAMS DATE AND TIME OF NECROPSY: 10/13/99 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); SHOULDER-RIGHT; WEEK 66; 10 LIVER (LI) : X 10MM; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >WART LIKE LESION(S); NOSE; WEEK 75; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: OBSERVED EOSINOPHILIC CELL,-SLIGHT >APPEARANCE; LIMITED USE; LIMBS-HIND; -HYPERPLASIA, BILE DUCT,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE SLIGHT PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); WITHIN MID PANCREAS: SINGLE -M-CARCINOMA, ISLET CELL,-NEOPLASM MASS, 10 X 7 X 5 MM, RED, DARK RED, PRESENT TAN, GRAY, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL 2070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 321 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92606 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/13/99 STUDY DAY OF DEATH: 542 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 669.7 GRAMS DATE AND TIME OF NECROPSY: 10/13/99 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); TIP OF SNOUT: SINGLE -ACANTHOSIS/HYPERKERATOSIS,-MODERATE DARK RED, DRY, SLIGHTLY RAISED CRUSTED -INFLAMMATION, CHRONIC-ACTIVE,- AREA, 2 X 1 MM (CAP 24); CONSISTENT MODERATELY SEVERE WITH WART-LIKE LESION (NOSE) AS NOTED -CYST, EPIDERMAL INCLUSION,-PRESENT IN CLINICAL OBSERVATIONS -MASS(ES); RIGHT SHOULDER REGION: SINGLE MASS, 20 X 18 X 6 MM, TAN AND FIRM; ON SECTION EXUDES TAN, CASEOUS SEMISOLID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PANCREAS,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 23) MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOW AND MOIST -------------------------------------------- 2071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 322 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92606 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/13/99 STUDY DAY OF DEATH: 542 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 669.7 GRAMS DATE AND TIME OF NECROPSY: 10/13/99 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 323 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92607 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 423.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:30 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES: BROWN AND TAN -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -GLYCOGEN, HEPATOCELLULAR, INCREASED,- MODERATE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -ROUGH SURFACE, DIFFUSE; BILATERAL MODERATELY SEVERE -DILATATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -PIGMENT,-MINIMAL TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE 2073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 324 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92607 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 423.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:30 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 12 X 12 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 10 X 5 MM -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 325 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92607 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 423.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:30 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 326 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92608 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 386.6 GRAMS DATE AND TIME OF NECROPSY: 10/19/99 14:16 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: OBSERVED SLIGHT >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; HUNCHED; VERIFIED AT SLIGHT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 327 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92608 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 386.6 GRAMS DATE AND TIME OF NECROPSY: 10/19/99 14:16 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; RIGHT: 20 X 10 X 3 MM (LEFT IS -SECRETION, INCREASED,-PRESENT 34 X 18 X 5 MM) -GELATINOUS; RIGHT: CLEAR AND SOFT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; BROWN AND YELLOW MOIST MATERIAL -------------------------------------------- 2077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 328 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92608 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 386.6 GRAMS DATE AND TIME OF NECROPSY: 10/19/99 14:16 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 329 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); HEAD-MAXILLARY- EYE (EY) : LEFT; WEEK 98; CORNER OF LEFT EYE; -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL VERIFIED AT NECROPSY: NOT OBSERVED BRAIN (BR) : BRAIN (BR) : >MASS(ES); DORSAL; WEEK 102; 10 X 10MM; -DEPRESSED AREA(S); LEFT FRONTAL -M-MENINGEAL SARCOMA,-NEOPLASM PRESENT VERIFIED AT NECROPSY: OBSERVED CORTEX: DUE TO MASS >EYES; CLEAR DISCHARGE; EYE-LEFT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; LIMITED USE; LIMBS-HIND; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >RESPIRATION; LABORED; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE 2079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 330 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: RED AND TAN >UNREMARKABLE HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 3 MM; APPEARS SWOLLEN -I-SARCOMA,-FROM BRAIN HARDERIAN GLAND (HG) : -I-SARCOMA,-FROM BRAIN SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC -B-FIBROMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 12 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE AXILLARY) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT: DARK RED (CAP -HEMORRHAGE,-PRESENT 22) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE CALVARIUM/SKULL (CA) : -I-SARCOMA,-FROM BRAIN 2080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 331 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CALVARIUM/SKULL (CA) : -MASS(ES); WITHIN CRANIAL VAULT, ATTACHED TO MENINGES ABOVE LEFT FRONTAL CORTEX: SINGLE MASS, 14 X 11 X 3 MM, TAN AND SOFT; COLLECTED INTACT WITH SKULL CAP GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: CLEAR AND MOIST MATERIAL -------------------------------------------- 2081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 332 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 333 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92610 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:57 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.5220 4.2417 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 334 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92610 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:57 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 335 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92611 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 988.9 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:47 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 85; 120 X 120MM; VERIFIED AT -DIFFUSELY LIGHT; ALL LOBES: LIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: OBSERVED BROWN EOSINOPHILIC CELL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -CYST, BILIARY,-PRESENT KIDNEY (KD) : -ECTASIA, TUBULAR,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE 2085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 336 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92611 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 988.9 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:47 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM X 3 X 3 MM) PRESENT PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); MID ASPECT: SINGLE -B-ADENOMA,-NEOPLASM PRESENT DARK RED AND TAN AREA, 2 X 1 X 1 MM SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT INGUINAL REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 140 X 100 X 60 MM (478.0 >NOTE:>SEE SKIN FOR DIAGNOSIS GRAMS), RED, DARK RED, TAN, LOBULATED, AND FIRM; SOME SECTIONS CONTAIN A DARK RED FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 337 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92611 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 988.9 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:47 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 338 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92612 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 476.5 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:11 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- NECROPSY: NOT APPLICABLE SLIGHT >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; SPLEEN (SP) : VERIFIED AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 8 X 5 X 5 MM (LEFT IS 4 -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM X 2 X 1 MM) PRESENT 2088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 339 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92612 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 476.5 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:11 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE AUDITORY SEB GL (AS) : AUDITORY SEB GL (AS) : -MASS(ES); RIGHT: SINGLE MASS, 17 X 10 -M-CARCINOMA,-NEOPLASM PRESENT X 8 MM, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH HEAD; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 2089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 340 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92612 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 476.5 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:11 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 341 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92613 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:28 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.1820 4.1251 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2091 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 342 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92613 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:28 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2092 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 343 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92614 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 7:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 89; 15 X 15MM; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED >AUTOLYTIC >SKIN & PELAGE; SORE/SCAB; DORSAL; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : >AUTOLYTIC ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2093 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 344 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92614 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 7:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : >AUTOLYTIC PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >AUTOLYTIC DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL CERVICAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE REGION: SINGLE DRY DARK RED CRUSTED -ULCERATION,-MODERATE AREA, 30 X 20 MM; COLLECTED; POSSIBLY -B-FIBROMA,-NEOPLASM PRESENT CONSISTENT WITH MASS (DORSAL) NOTED IN CLINICAL OBSERVATION URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT 2094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 345 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92614 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 7:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -CONGESTION,-PRESENT COLLECTED -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 346 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92615 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 633.0 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:04 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 18 X 15 X 5 MM (LEFT IS -ATROPHY/DEGENERATION,-MODERATELY 23 X 15 X 10 MM); BOTH ARE SOFT SEVERE -CONTAINS FLUID; BILATERAL: CLEAR >NOTE:>BILATERAL FLUID 2096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 347 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92615 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 633.0 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:04 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 17 X 11 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY -------------------------------------------- 2097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 348 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92615 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 633.0 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:04 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 349 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92616 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 401.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:26 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0196 .0049 % .0094 WEIGHT TAKEN RT ADRENAL (AD1) .0158 .0039 % .0076 WEIGHT TAKEN BRAIN (BR) 2.0788 .5181 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.5410 .3841 % .7413 WEIGHT TAKEN RT KIDNEY (KD1) 1.6127 .4020 % .7758 WEIGHT TAKEN LIVER (LI) 11.5117 2.8693 % 5.5377 WEIGHT TAKEN LUNG (LU) 1.7953 .4475 % .8636 WEIGHT TAKEN SPLEEN (SP) .5603 .1397 % .2695 WEIGHT TAKEN LF TESTIS (TE0) 1.5787 .3935 % .7594 WEIGHT TAKEN RT TESTIS (TE1) 1.5658 .3903 % .7532 WEIGHT TAKEN LF THYROID/PARA (TT0) .0102 .0025 % .0049 WEIGHT TAKEN RT THYROID/PARA (TT1) .0099 .0025 % .0048 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL HARDERIAN GLAND (HG) : HARDERIAN GLAND (HG) : -MISSING/NOT IDENTIFIED; LEFT: NOT >NOT REQUIRED TO BE EXAMINED FOR ANIMAL IDENTIFIED; RIGHT APPEARS NORMAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 350 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92616 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 401.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:26 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN PINPOINT FOCI CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 351 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92617 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 565 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 7:09 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; PALE; EYES; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -WHITE INTRAOCULAR MATERIAL; -DEGENERATION, LENTICULAR,-MODERATE >APPEARANCE; THIN; VERIFIED AT BILATERAL; POSSIBLY CONSISTENT WITH NECROPSY: OBSERVED PALE EYES AS NOTED IN CLINICAL >EXCRETION; NO FECES; VERIFIED AT OBSERVATIONS NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT AT NECROPSY: NOT APPLICABLE -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT SURFACE OF LEFT LATERAL LOBE: SINGLE -VACUOLATION, HEPATOCELLULAR, NECROPSY: OBSERVED TAN AREA, 17 X 11 MM; COLLECTED ON PERIPORTAL,-SLIGHT ROUTINE SECTION -HYPERPLASIA, BILE DUCT,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 2101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 352 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92617 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 565 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 7:09 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -INFLAMMATION, SUBACUTE,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 9 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN, LOBULATED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA, NEAR -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PYLORIC VALVE: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR AND SOFT >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 2102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 353 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92617 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 565 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 7:09 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 354 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92618 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:23 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 14.8145 4.6341 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 355 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92618 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:23 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 356 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92619 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 684.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERPLASIA, ISLET CELLS,-SLIGHT -ATROPHY, ACINAR,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 357 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92619 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 684.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 358 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92620 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 8 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, GRAY AND SOFT 2108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 359 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92620 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -EDEMA,-SLIGHT -INFLAMMATION, ACUTE,-SLIGHT COLON (CO) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT PREPUTIAL GLAND (PG) : PREPUTIAL GLAND (PG) : -LARGE; BILATERAL: LEFT IS 6 X 6 X 3 -INFLAMMATION, CHRONIC-ACTIVE,- MM, RIGHT IS 5 X 4 X 3 MM; BOTH APPEAR MODERATELY SEVERE CYSTIC WITH GREEN SEMISOLID (CAP 22) GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL: LOWER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 2109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 360 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92620 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 361 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92621 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0379 .0072 % .0175 WEIGHT TAKEN RT ADRENAL (AD1) .0377 .0072 % .0174 WEIGHT TAKEN BRAIN (BR) 2.1646 .4109 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.9881 .3774 % .9185 WEIGHT TAKEN RT KIDNEY (KD1) 1.8534 .3518 % .8562 WEIGHT TAKEN LIVER (LI) 18.7242 3.5543 % 8.6502 WEIGHT TAKEN LUNG (LU) 1.9337 .3671 % .8933 WEIGHT TAKEN SPLEEN (SP) .8626 .1637 % .3985 WEIGHT TAKEN LF TESTIS (TE0) 1.6258 .3086 % .7511 WEIGHT TAKEN RT TESTIS (TE1) 1.6364 .3106 % .7560 WEIGHT TAKEN LF THYROID/PARA (TT0) .0117 .0022 % .0054 WEIGHT TAKEN RT THYROID/PARA (TT1) .0145 .0028 % .0067 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT MISSING FROM WET TISSUE AT TIME OF TRIMMING 2111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 362 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92621 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : ADRENAL, MEDULLA (MA) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT MISSING FROM WET TISSUE AT TIME OF TRIMMING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED >NOT REQUIRED TO BE EXAMINED FOR ANIMAL (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>SODIUM PENTOBARBITAL ADMINISTERED PRIOR TO CARBON DIOXIDE ANESTHESIA -------------------------------------------- 2112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 363 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92621 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 364 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92622 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 7:52 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -ANGIECTASIS,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED -HEMORRHAGE,-PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 2114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 365 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92622 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 7:52 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : HEART (HT) : -MASS(ES); INVOLVING RIGHT AND LEFT -M-ATRIOCAVAL MESOTHELIOMA,-NEOPLASM ATRIA: SINGLE MASS, 12 MM IN DIAMETER, PRESENT TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -FIBROSIS,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, HEART,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) -HEMORRHAGE,-PRESENT -INFILTRATE, MAST CELL,-MODERATE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 2115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 366 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92622 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 7:52 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 367 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92623 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:59 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 5 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-MINIMAL SECTIONS -DEGENERATION, CYSTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR -HYPERPLASIA,-SLIGHT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT 2117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 368 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92623 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:59 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 369 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92624 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/18/99 14:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 2119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 370 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92624 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/18/99 14:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-NASAL; BILATERAL: RED AND MOIST MATERIAL -------------------------------------------- 2120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 371 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92624 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/18/99 14:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 372 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT OBSERVED EOSINOPHILIC CELL,-MINIMAL >SKIN & PELAGE; PALE; ENTIRE BODY; -DEGENERATION, CYSTIC,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >APPEARANCE; TREMORS; HEAD; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -CYST(S); BILATERAL, CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE MULTIPLE CLEAR FLUID FILLED CYSTS, UP SEVERE >APPEARANCE; HUNCHED; VERIFIED AT TO 10 MM IN DIAMETER -DILATATION, PELVIC,-SLIGHT NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; BILATERAL >RESPIRATION; IRREGULAR; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE >BEHAVIOR; HYPOACTIVE; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL 2122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 373 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATELY SEVERE LN, MESENTERIC (MS) : >TISSUE MISSING TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED FOCUS, 1 MM IN -CYST,-PRESENT DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 374 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT -MINERALIZATION, GLANDULAR MUCOSA,- MODERATELY SEVERE CECUM (CE) : -EROSION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 375 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 376 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92626 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 726.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:55 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; MISSING; TEETH; VERIFIED -FOCUS, ALTERED HEPATOCELLULAR, AT NECROPSY: OBSERVED BASOPHILIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -HYPERPLASIA, BILE DUCT,-MINIMAL LEFT; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: OBSERVED MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,- HINDFOOT: SINGLE MOIST, RED ABRADED MODERATELY SEVERE AREA, 15 MM IN DIAMETER; COLLECTED -ULCERATION,-MODERATE 2126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 377 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92626 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 726.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:55 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL: UP TO 12 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: MISSING -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -------------------------------------------- 2127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 378 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92626 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 726.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:55 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 379 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92627 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 525.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:32 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0277 .0053 % .0119 WEIGHT TAKEN RT ADRENAL (AD1) .0262 .0050 % .0113 WEIGHT TAKEN BRAIN (BR) 2.3192 .4415 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.0656 .3932 % .8907 WEIGHT TAKEN RT KIDNEY (KD1) 1.9527 .3717 % .8420 WEIGHT TAKEN LIVER (LI) 16.9241 3.2218 % 7.2974 WEIGHT TAKEN LUNG (LU) 1.8755 .3570 % .8087 WEIGHT TAKEN SPLEEN (SP) 1.0427 .1985 % .4496 WEIGHT TAKEN LF TESTIS (TE0) 2.0320 .3868 % .8762 WEIGHT TAKEN RT TESTIS (TE1) 1.9782 .3766 % .8530 WEIGHT TAKEN LF THYROID/PARA (TT0) .0148 .0028 % .0064 WEIGHT TAKEN RT THYROID/PARA (TT1) .0192 .0037 % .0083 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 2129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 380 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92627 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 525.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:32 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 381 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92628 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 471.9 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT EYE (EY) : NECROPSY: NOT OBSERVED >NOTE:>LEFT RUPTURED AT NECROPSY >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; BILATERAL: EACH IS 3 X 2 X 2 -PIGMENT,-SLIGHT MM -ATROPHY, CORTICAL,-MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 2131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 382 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92628 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 471.9 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE TAN ERODED AREA, 2 X 1 MM -THICKENED WALL; DIFFUSE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN, BROWN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 25 X 15 X 5 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 383 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92628 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 471.9 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 384 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -LARGE; BILATERAL: EACH IS 32 X 26 X SEVERE 19 MM -DIFFUSELY LIGHT; BILATERAL: TAN SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -B-ADENOMA, ACINAR CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT -VACUOLATION, FOCAL,-SLIGHT IS 4 MM IN DIAMETER) -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 19 X 15 X -ATROPHY/DEGENERATION,-MODERATELY 15 MM; BOTH ARE SOFT SEVERE -INFLAMMATION, VASCULAR,-SLIGHT 2134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 385 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -LEUKOCYTES, INCREASED, INTRAVASCULAR,- PRESENT -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE PARATHYROID (PT) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MULTIPLE RED PINPOINT FOCI SLIGHT -THICKENED WALL; MUCOSA -MINERALIZATION, GLANDULAR MUCOSA,- MODERATE ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT 2135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 386 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 5 MM IN -MINERALIZATION,-MODERATELY SEVERE DIAMETER -FIRM; DIFFUSE GENERAL COMMENT (GC) : -ANIMAL THIN -URINARY BLADDER-LARGE LUMEN; UP TO 18 MM IN DIAMETER -DISCHARGE-ORAL; CLEAR, MOIST MATERIAL -------------------------------------------- 2136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 387 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 388 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 73; 10 X 10MM; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT NECROPSY: OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -CYST(S); BILATERAL, DIFFUSE: MULTIPLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; ATAXIC; VERIFIED AT CLEAR FLUID FILLED CYSTS, UP TO 3 MM SEVERE NECROPSY: NOT APPLICABLE IN DIAMETER -HYPERPLASIA, TRANSITIONAL CELL,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LARGE PELVIS(ES); BILATERAL MODERATE NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; BILATERAL: TAN -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- SLIGHT -CYST,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 6 MM (RIGHT IS -VACUOLATION, FOCAL,-SLIGHT 5 X 4 X 4 MM) -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : >TISSUE MISSING 2138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 389 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 20 X 15 X 8 MM (LEFT IS -ATROPHY/DEGENERATION,-MODERATELY 25 X 17 X 10 MM); RIGHT IS SOFT SEVERE -INFLAMMATION, VASCULAR,-SLIGHT >NOTE:>UNILATERAL BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATELY SEVERE SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 17 X 10 X 3 -ATROPHY,-MODERATELY SEVERE MM 2139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 390 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 22) MODERATE MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); RIGHT PERINEAL REGION: TWO >NOT REQUIRED TO BE EXAMINED FOR ANIMAL TAN AND CREAMY SEMIFLUID FILLED CYSTS, >NOTE:>SEE SKIN FOR DIAGNOSIS ONE IS 15 MM IN DIAMETER, THE OTHER IS 5 MM IN DIAMETER; COLLECTED; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT FILLED CYST, 6 MM IN DIAMETER (CAP 23) -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 2140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 391 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2141 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume VI of IX Page 2142 of 4068 Covance 6329-183 3M T-6295 VOLUME VI OF IX CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 2144 Individual Anatomic Pathology Data ..................................................................... 2145 2143 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 2144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 392 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92631 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE 2145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 393 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92631 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 394 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92631 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 395 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; EAR-LEFT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); EAR-LEFT; WEEK 48; 30 X -HYPERPLASIA, BILE DUCT,-MINIMAL 30MM; VERIFIED AT NECROPSY: OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK KIDNEY (KD) : 103; 25 X 45MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED SLIGHT >APPEARANCE; THIN; VERIFIED AT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : NECROPSY: NOT OBSERVED -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT -VACUOLATION, FOCAL,-SLIGHT >RESPIRATION; LABORED; VERIFIED AT IS 4 MM IN DIAMETER) NECROPSY: NOT APPLICABLE ADRENAL, MEDULLA (MA) : >EXCRETION; FEW FECES; VERIFIED AT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE TESTIS (TE) : >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -ATROPHY/DEGENERATION,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT -DEPLETION, LYMPHOCYTIC (GENERALLY IDENTIFIED: POSSIBLE REMNANTS COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- COLLECTED MODERATELY SEVERE 2148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 396 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -MINERALIZATION, VASCULAR,-MINIMAL MULTIPLE GRAY, FIRM AND SLIGHTLY -N-SARCOMA,-NEOPLASM PRESENT RAISED AREAS, UP TO 9 X 7 X 3 MM HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF -CARDIOMYOPATHY,-SLIGHT LEFT VENTRICLE: SINGLE TAN AREA, 3 X 2 -N-SARCOMA,-NEOPLASM PRESENT X 1 MM THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 4 X 3 X 2 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : -I-SARCOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE >UNREMARKABLE SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT AUDITORY SEB GL (AS) : -I-SARCOMA,-NEOPLASM PRESENT 2149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 397 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ AUDITORY SEB GL (AS) : -MASS(ES); LEFT, INVOLVING ENTIRE ORGAN, EXTENDING TO LEFT VENTRAL CERVICAL REGION AND LEFT PINNA: SINGLE MASS, 57 X 23 X 18 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22); SKIN OVERLYING LEFT PINNA HAS A SINGLE DRY BLACK CRUSTED AREA, 28 X 25 X 19 MM; COLLECTED INTACT WITH MASS PERICARDIAL SAC (PD) : PERICARDIAL SAC (PD) : -MASS(ES); INVOLVING ENTIRE TISSUE: -N-SARCOMA,-NEOPLASM PRESENT SINGLE MASS, 34 X 24 X 14 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED; PORTIONS OF MASS ADHERED TO DIAPHRAGM; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 398 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 399 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92633 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 830.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:20 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -VACUOLATION, HEPATOCELLULAR, RIGHT; VERIFIED AT NECROPSY: OBSERVED PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- MODERATELY SEVERE TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 20 X 13 X 4 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE 25 X 15 X 12 MM); LEFT APPEARS SOFT >NOTE:>UNILATERAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 2152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 400 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92633 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 830.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:20 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT AND RIGHT -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA ON EACH, BOTH ARE 2 X 1 MM STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT DARK BROWN ERODED AREA, 4 X 1 MM -ULCERATION,-SLIGHT -THICKENED WALL; MUCOSA: DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE DARK RED AND DRY AREA, 6 X 5 MODERATELY SEVERE MM; COLLECTED -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 2153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 401 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92633 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 830.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:20 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 402 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92634 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/13/99 7:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>BILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL 2155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 403 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92634 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/13/99 7:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, RED, GREY, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 2156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 404 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92634 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/13/99 7:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), SKIN (SK), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 405 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92635 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 481.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:50 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- EYE (EY) : ENTIRE; VERIFIED AT NECROPSY: NOT -DYSTROPHY, CORNEAL IN EARLY STAGES, OBSERVED CONSISTS PRIMARILY OF FOCAL >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK MINERALIZATION,-MINIMAL 76; 20 X 15MM; VERIFIED AT NECROPSY: BRAIN (BR) : BRAIN (BR) : OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >EXCRETION; NONFORMED FECES; VERIFIED PERIPORTAL,-SLIGHT AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 2158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 406 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92635 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 481.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:50 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; RIGHT: CLEAR AND SOFT -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -MASS(ES); RIGHT LATERAL MANDIBLE, >UNREMARKABLE NEAR BASE OF PINNA: SINGLE MASS, 17 MM >NOTE:>SEE AUDITORY SEBACEOUS GLAND FOR IN DIAMETER, TAN AND FIRM; ON SECTION DIAGNOSIS EXUDES A TAN, CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 407 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92635 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 481.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:50 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 408 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; TESTES; VERIFIED BRAIN (BR) : AT NECROPSY: OBSERVED -MINERALIZATION,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- LIVER (LI) : LIVER (LI) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES, DIFFUSE: RED, -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED DARK RED AND TAN -DEGENERATION, CYSTIC,-MINIMAL -CONGESTION,-PRESENT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, EOSINOPHILIC,-MODERATE -FIBROSIS,-SEVERE -INFLAMMATION, GRANULOMATOUS,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MOTTLED; DIFFUSE: RED, TAN AND GREEN -INFILTRATE, EOSINOPHILIC,-MODERATE -INFLAMMATION, GRANULOMATOUS,-MODERATE 2161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 409 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; BILATERAL: CLEAR -ATROPHY/DEGENERATION,-SEVERE FLUID MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT -INFLAMMATION, GRANULOMATOUS,-SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -CYST(S); LEFT ASPECT: SINGLE CYST, 2 -B-ADENOMA,-NEOPLASM PRESENT X 3 MM, FILLED WITH A DARK RED FLUID ILEUM (IL) : -INFILTRATE, EOSINOPHILIC,-SEVERE -INFLAMMATION, GRANULOMATOUS,-SEVERE COLON (CO) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFILTRATE, EOSINOPHILIC,-SEVERE 2162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 410 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 3 X 6 X 9 MM (CAP 26) -HEMORRHAGE,-PRESENT -DIFFUSELY RED; DARK RED (CAP 26) -INFLAMMATION, GRANULOMATOUS,-SEVERE -INFILTRATE, EOSINOPHILIC,-MODERATE -FIBROSIS,-MODERATE LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 3 X 5 X 8 -HEMORRHAGE,-PRESENT MM (CAP 27) -HYPERPLASIA, LYMPHOPLASMACYTIC,- -DIFFUSELY RED; BILATERAL (CAP 27) MODERATE LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 25) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS 5 X 7 X 11 -LYMPHANGIECTASIS,-PRESENT MM (CAP 28) -HEMORRHAGE,-PRESENT -DIFFUSELY RED; BILATERAL (CAP 28) -INFLAMMATION, SUBACUTE,-SLIGHT PREPUTIAL GLAND (PG) : PREPUTIAL GLAND (PG) : -LARGE; LEFT: 10 X 10 X 15 MM (RIGHT -INFLAMMATION, CHRONIC-ACTIVE,- IS 3 X 5 X 9 MM); LEFT IS FILLED WITH MODERATELY SEVERE A LIGHT GREEN SEMIFLUID; OVERLYING -ECTASIA, DUCTAL,-PRESENT SKIN HAS A SINGLE DRY, BROWN CRUSTED AREA, 4 X 6 MM (CAP 23) ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -THICKENED; ATTACHED TO BOTH VAS -INFILTRATE, EOSINOPHILIC,-MODERATE DEFERENS AND EPIDIDYMIDES: FIRM, RED, -INFLAMMATION, GRANULOMATOUS,- TAN AND FIBROUS (CAP 24) MODERATELY SEVERE 2163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 411 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MUSCLE, OTHER (MO) : MUSCLE, OTHER (MO) : -THICKENED; MUSCLE ASSOCIATED WITH -INFLAMMATION, GRANULOMATOUS,- SCROTUM: UP TO 4 MM THICK (CAP 22) MODERATELY SEVERE -DIFFUSELY RED; MUSCLE ASSOCIATED WITH -INFILTRATE, EOSINOPHILIC,-MODERATE SCROTUM: DARK RED (CAP 22) -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 412 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92637 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -HYPERTROPHY, FOLLICULAR CELL,- MODERATELY SEVERE 2165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 413 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92637 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); MID ANTERIOR ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE TAN AREA, 1 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 414 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; PALE; ENTIRE BODY; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 5 MM IN -ZYMOGEN GRANULES, DECREASED,-MODERATE DIAMETER (CAP 23) -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT -FIBROSIS,-SLIGHT 2167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 415 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATELY SEVERE -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM; RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT VASCULAR AND FIRM PROSTATE (PR) : PROSTATE (PR) : -NODULAR; TISSUE SURROUNDING URETHRA: >UNREMARKABLE GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 34 X 14 X 6 -SECRETION, INCREASED,-PRESENT MM -GELATINOUS; BILATERAL: CLEAR AND SOFT 2168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 416 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS 10 X 6 X 6 -HEMORRHAGE,-PRESENT MM (CAP 22) -DILATATION, SINUSOIDAL,-SEVERE -DIFFUSELY RED; BILATERAL: DARK RED AORTA (AO) : AORTA (AO) : -LARGE; UP TO 5 MM IN DIAMETER; >UNREMARKABLE COLLECTED -FIRM; ALONG ENTIRE LENGTH GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 417 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 418 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92639 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 486.0 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:10 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 419 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92639 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 486.0 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:10 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); SINGLE MASS, 14 X 8 X 7 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM JEJUNUM (JE) : >TISSUE MISSING PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 420 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92639 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 486.0 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:10 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 421 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92640 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:27 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0426 .0110 % .0175 WEIGHT TAKEN RT ADRENAL (AD1) .0356 .0092 % .0146 WEIGHT TAKEN BRAIN (BR) 2.4406 .6276 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.9384 .4984 % .7942 WEIGHT TAKEN RT KIDNEY (KD1) 1.9241 .4948 % .7884 WEIGHT TAKEN LIVER (LI) 12.6645 3.2565 % 5.1891 WEIGHT TAKEN LUNG (LU) 1.8519 .4762 % .7588 WEIGHT TAKEN SPLEEN (SP) .8027 .2064 % .3289 WEIGHT TAKEN LF TESTIS (TE0) 1.7129 .4404 % .7018 WEIGHT TAKEN RT TESTIS (TE1) 1.6810 .4322 % .6888 WEIGHT TAKEN LF THYROID/PARA (TT0) .0298 .0077 % .0122 WEIGHT TAKEN RT THYROID/PARA (TT1) .0134 .0034 % .0055 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 422 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92640 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:27 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 423 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92641 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 613.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -MINERALIZATION,-MINIMAL AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 5 MM IN PERIPORTAL,-MINIMAL DIAMETER; COLLECTED ON ROUTINE -ANGIECTASIS,-SLIGHT SECTIONS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,- MODERATELY SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, SOFT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,- MODERATE 2176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 424 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92641 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 613.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 2177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 425 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92641 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 613.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 426 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92642 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 799.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:32 PROSECTOR: JERALD W. JOHNSON RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL -VACUOLATION, HEPATOCELLULAR, LOBE: MULTIPLE DARK RED AREAS, UP TO 2 PERIPORTAL,-SLIGHT MM IN DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / SECTION EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); NEAR DUODENUM: SINGLE MASS, -INFILTRATE, MACROPHAGE, PIGMENTED,- 8 MM IN DIAMETER, RED, TAN, AND FIRM; MINIMAL SAME ON CUT SURFACE (CAP 22); REGIONAL -M-CARCINOMA, ISLET CELL,-NEOPLASM LN (SEE PANCREATIC) PRESENT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT 2179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 427 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92642 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 799.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:32 PROSECTOR: JERALD W. JOHNSON RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN -HYPERPLASIA,-SLIGHT -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR -CYST,-PRESENT ASPECT: SINGLE DARK BROWN AREA, 3 X 2 MM URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT PREPUTIAL GLAND (PG) : PREPUTIAL GLAND (PG) : -LARGE; RIGHT: 8 X 7 X 7 MM (LEFT IS 8 -INFLAMMATION, CHRONIC-ACTIVE,- X 6 X 2 MM); RIGHT CONTAINS A GREEN MODERATELY SEVERE SEMISOLID (CAP 24) -ECTASIA, DUCTAL,-PRESENT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 23) -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 428 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92642 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 799.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:32 PROSECTOR: JERALD W. JOHNSON RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 429 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92643 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 714.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:47 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK LIVER (LI) : 101; 60 X 60MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, MIDZONAL OBSERVED / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SEVERE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: -B-ADENOMA,-NEOPLASM PRESENT MULTIPLE DARK RED AREAS, UP TO 3 X 1 MM SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT 2182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 430 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92643 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 714.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:47 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 80 X 65 X 30 MM, RED, >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) RIGHT NOT IDENTIFIED, LEFT IS NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 431 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92643 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 714.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:47 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 432 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92644 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 658.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:01 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 104; VERIFIED LIVER (LI) : AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); LEFT WITHIN CORTEX: SINGLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- CLEAR FLUID FILLED CYST, 4 X 5 MM MODERATE -M-RENAL MESENCHYMAL TUMOR,-NEOPLASM PRESENT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 3 X 1 MM SKIN (SK) : SKIN (SK) : -RAISED FOCUS(I)/AREA(S); LUMBAR -B-ADENOMA, BASAL REGION: SINGLE DARK RED AND TAN RAISED CELL/SQUAMOUS/SEBACEOUS,-NEOPLASM AREA, 10 X 10 X 6 MM (CAP 22); PRESENT POSSIBLY CONSISTENT WITH MASS (DORSAL) AS NOTED IN CLINICAL OBSERVATIONS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 433 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92644 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 658.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:01 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: BROWN, GREEN, AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -GI TRACT-DARK FLUID CONTENT; ILEUM AND CECUM: DARK GREEN SEMIFLUID -------------------------------------------- 2186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 434 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92644 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 658.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:01 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 435 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92645 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:40 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0336 .0071 % .0153 WEIGHT TAKEN RT ADRENAL (AD1) .0367 .0077 % .0167 WEIGHT TAKEN BRAIN (BR) 2.1979 .4630 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.1058 .4436 % .9581 WEIGHT TAKEN RT KIDNEY (KD1) 2.1132 .4452 % .9615 WEIGHT TAKEN LIVER (LI) 16.1865 3.4098 % 7.3645 WEIGHT TAKEN LUNG (LU) 1.8951 .3992 % .8622 WEIGHT TAKEN SPLEEN (SP) .8506 .1792 % .3870 WEIGHT TAKEN LF TESTIS (TE0) 1.7410 .3668 % .7921 WEIGHT TAKEN RT TESTIS (TE1) 1.7661 .3720 % .8035 WEIGHT TAKEN LF THYROID/PARA (TT0) .0117 .0025 % .0053 WEIGHT TAKEN RT THYROID/PARA (TT1) .0131 .0028 % .0060 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 436 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92645 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:40 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 437 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92646 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:56 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.3520 3.6159 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 438 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92646 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:56 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 439 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92647 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 9:52 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT PERIPORTAL,-MODERATE NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 11 X 6 >UNREMARKABLE MM; BOTH ARE SOFT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 2192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 440 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92647 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 9:52 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -INFLAMMATION, ACUTE,-MINIMAL TAN ERODED AREAS, UP TO 4 MM IN DIAMETER STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : PROSTATE (PR) : -SMALL; 17 X 8 X 4 MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 6 X 2 -ATROPHY,-SEVERE MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST YELLOW AND BROWN MATERIAL -------------------------------------------- 2193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 441 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92647 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 9:52 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 442 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92648 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 411.7 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); VISCERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE SURFACE OF RIGHT MEDIAN LOBE, ALONG -FOCUS, ALTERED HEPATOCELLULAR, MARGIN: SINGLE TAN AREA, 4 X 3 MM; BASOPHILIC,-MINIMAL COLLECTED ON ROUTINE SECTION -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 2195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 443 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92648 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 411.7 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 9 MM, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- TAN AND BROWN ERODED AREAS, UP TO 8 X MODERATELY SEVERE 5 MM -ULCERATION,-MODERATE -THICKENED WALL; MUCOSA, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE PROSTATE (PR) : PROSTATE (PR) : -LARGE; 30 X 20 X 12 MM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -GELATINOUS; CLEAR AND SOFT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 36 X 15 X -SECRETION, INCREASED,-PRESENT 11 MM 2196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 444 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92648 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 411.7 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 445 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92649 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OF MEDIAN LOBES: SINGLE TAN AREA, 6 X -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 4 MM; COLLECTED EOSINOPHILIC CELL,-SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 446 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92649 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 447 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92650 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.2299 4.1391 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 448 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92650 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 449 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; SWOLLEN; TAIL-PROXIMAL; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT NECROPSY: OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED SLIGHT AT NECROPSY: NOT APPLICABLE SPLEEN (SP) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -PIGMENT, INCREASED,-MODERATE NECROPSY: NOT APPLICABLE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -SMALL; UP TO 2 MM IN DIAMETER >UNREMARKABLE THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT -DEPLETION, LYMPHOCYTIC (GENERALLY POSITIVELY IDENTIFIED; POSSIBLE COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- REMNANTS COLLECTED SEVERE 2202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 450 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 11 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, LOBULATED, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE >UNREMARKABLE TAN AND BROWN SLIGHTLY ERODED AREAS, UP TO 3 X 2 MM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 31 X 21 X 6 -SECRETION, INCREASED,-PRESENT MM; BOTH APPEAR GELATINOUS EPIDIDYMIDES (EP) : >UNREMARKABLE >NOTE:>VAS DEFERENS - UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT TAIL (TI) : TAIL (TI) : -LARGE; AT BASE: UP TO 15 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL DIAMETER; APPEARS FIRM AND RIGID; PORTION COLLECTED LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE 2203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 451 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE >NOTE:>GROSS RECORDED UNDER ADIPOSE TISSUE JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT, TARSAL: 15 X 11 X 11 MM -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE (LEFT IS 10 X 9 X 8 MM); BOTH COLLECTED ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -MASS(ES); TISSUE SURROUNDING LEFT VAS >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DEFERENS: SINGLE FIRM AND YELLOW MASS, 17 X 8 X 3 MM; ON SECTION, EXUDES A YELLOW CASEOUS SEMIFLUID MATERIAL (CAP 22); REGIONAL LN (LUMBAR) NORMAL (CAP 23) VAS DEFERENS (VS) : VAS DEFERENS (VS) : -LARGE; BILATERAL: EACH IS UP TO 5 MM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL IN DIAMETER; BOTH APPEAR SOFT AND GELATINOUS (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 452 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 453 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92652 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 292.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:31 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.5416 3.9468 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 454 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92652 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 292.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:31 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 455 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92653 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 771.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 3 MM IN -NECROSIS, INDIVIDUAL HEPATOCYTE,- DIAMETER; COLLECTED ON ROUTINE MINIMAL SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 17 X 12 X 8 MM (RIGHT IS -ATROPHY/DEGENERATION,-SLIGHT 22 X 14 X 10 MM) -INFLAMMATION, VASCULAR,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 456 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92653 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 771.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, AND SOFT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: YELLOW AND >UNREMARKABLE CLEAR CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 457 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92653 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 771.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 458 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; OPAQUE; EYE-LEFT; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -OPAQUE CORNEA; LEFT -I-NEUROFIBROSARCOMA,-NEOPLASM PRESENT >EYES; SCAB; EYE-LEFT; VERIFIED AT -PROTRUDING; LEFT NECROPSY: NOT OBSERVED -LARGE; LEFT: 9 MM IN DIAMETER (RIGHT >EYES; PROTRUDING; EYE-LEFT; VERIFIED IS 6 MM IN DIAMETER) AT NECROPSY: OBSERVED BRAIN (BR) : >EXCRETION; NO FECES; VERIFIED AT -M-NEUROFIBROSARCOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL 2211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 459 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE PITUITARY (PI) : -HYPERPLASIA,-SLIGHT -CYST,-PRESENT HARDERIAN GLAND (HG) : HARDERIAN GLAND (HG) : -MISSING/NOT IDENTIFIED; BILATERAL: >TISSUE MISSING NOT POSITIVELY IDENTIFIED DUE TO CALVARIUM MASS; POSSIBLE REMNANTS COLLECTED STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; LEFT: UP TO 12 X 8 X 4 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (RIGHT IS UP TO 6 X 4 X 2 MM); MODERATE COLLECTED -LYMPHANGIECTASIS,-PRESENT 2212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 460 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CALVARIUM/SKULL (CA) : CALVARIUM/SKULL (CA) : -MASS(ES); BILATERAL ORBITS, EXTENDING -I-SARCOMA,-NEOPLASM PRESENT INTO CRANIAL VAULT: SINGLE MASS, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY MATERIAL -------------------------------------------- 2213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 461 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 462 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-MID; WEEK 85; 20 X BRAIN (BR) : BRAIN (BR) : 25MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; TAIL-MID; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE 2215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 463 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFLAMMATION, GRANULOMATOUS,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, LOBULATED, AND SOFT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE -ULCERATION,-MODERATELY SEVERE -M-NEUROFIBROSARCOMA,-NEOPLASM PRESENT 2216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 464 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : -MASS(ES); 35 MM FROM BASE: SINGLE MASS, 35 X 32 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22); OVERLYING SKIN HAS A SINGLE, DARK RED ULCERATED AREA, 20 MM IN DIAMETER; COLLECTED INTACT WITH MASS LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 465 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 466 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL- BRAIN (BR) : BRAIN (BR) : THORACIC; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL NECROPSY: NOT APPLICABLE -FIBROSIS, PERIBILIARY,-SLIGHT >SKIN & PELAGE; PALE; ENTIRE BODY; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -CYST(S); BILATERAL, CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- MULTIPLE PINPOINT, CLEAR FLUID FILLED SEVERE CYSTS -INFLAMMATION, SUPPURATIVE, PELVIC,- -ROUGH SURFACE, DIFFUSE; BILATERAL MINIMAL -DIFFUSELY LIGHT; BILATERAL: TAN -THROMBUS,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -ATROPHY, ACINAR,-MINIMAL -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL 2219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 467 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 10 X 5 -ATROPHY/DEGENERATION,-MINIMAL MM -INFLAMMATION, VASCULAR,-SLIGHT BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF -CARDIOMYOPATHY,-MODERATELY SEVERE BOTH VENTRICLES: MULTIPLE TAN, -MINERALIZATION, VASCULAR,-SEVERE INDISTINCT AREAS THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 MM IN -HYPERPLASIA,-SEVERE DIAMETER PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 468 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, GRAY, TAN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -MINERALIZATION, GLANDULAR MUCOSA,- MULTIPLE BROWN AREAS, UP TO 3 X 2 MM SLIGHT -THICKENED WALL; DIFFUSE SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); LEFT DORSAL THORACIC -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE TAN, LOBULATED CRUSTED AREA, 18 X 10 MM (CAP 25) URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: -HYPERPLASIA, CYSTIC,-MODERATE MULTIPLE TAN, CREAMY SEMIFLUID FILLED -GALACTOCELE,-PRESENT CYSTS, UP TO 5 MM IN DIAMETER (CAP 22) AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 8 MM IN -MINERALIZATION,-MODERATELY SEVERE DIAMETER; COLLECTED -FIRM; DIFFUSE 2221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 469 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ARTERY, OTHER (AU) : ARTERY, OTHER (AU) : -LARGE; BILATERAL, CAROTID: EACH IS UP -MINERALIZATION,-MODERATELY SEVERE TO 3 MM IN DIAMETER AND BOTH ARE FIRM (CAP 23) AORTIC ARCH (AA) : AORTIC ARCH (AA) : -LARGE; UP TO 10 MM IN DIAMETER AND -MINERALIZATION,-MODERATELY SEVERE FIRM (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 470 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 471 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92657 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:39 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); SHOULDER-LEFT; LIVER (LI) : WEEK 90; VERIFIED AT NECROPSY: NOT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: -HYPERPLASIA,-MODERATE SINGLE DARK RED AREA, 3 MM IN DIAMETER SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 472 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92657 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:39 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -MASS(ES); LEFT LATERAL THORACIC REGION: SINGLE MASS, 12 X 12 X 10 MM, TAN AND HARD; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DRY, TAN, CRUSTED, ULCERATED AREA, 10 MM IN DIAMETER (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 473 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92657 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:39 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 474 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92658 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 14:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL -NECROSIS, FOCAL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 475 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92658 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 14:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 3 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY AND SOFT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: LEFT IS RED, RIGHT IS RED AND BROWN -ANIMAL OBESE -------------------------------------------- 2228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 476 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92658 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 14:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 477 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92659 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 705.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; MALOCCLUSION; VERIFIED AT BIFURCATION OF RIGHT AND LEFT MEDIAN -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT OBSERVED LOBE: SINGLE TAN AREA, 4 X 3 MM (CAP CENTRILOBULAR POLYKARYOCYTOSIS 22) OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 2230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 478 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92659 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 705.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 2 MM IN -HYPERPLASIA,-MODERATE DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: 15 MM IN -INFLAMMATION, CHRONIC-ACTIVE,- DIAMETER (RIGHT IS 9 MM IN DIAMETER); MODERATELY SEVERE COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 479 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92659 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 705.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 480 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92660 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 659.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 2 MM IN -FOCUS, ALTERED HEPATOCELLULAR, DIAMETER, COLLECTED ON ROUTINE BASOPHILIC,-MINIMAL SECTIONS -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 481 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92660 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 659.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 482 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >SKIN & PELAGE; PALE; ENTIRE BODY; CONSISTS PRIMARILY OF FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE MINERALIZATION,-SLIGHT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -MINERALIZATION,-MINIMAL LIVER (LI) : LIVER (LI) : -MASS(ES); RIGHT LATERAL LOBE: SINGLE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT PEDUNCULATED MASS, 40 X 20 X 10 MM, -VACUOLATION, HEPATOCELLULAR, MIDZONAL DARK RED, BROWN, TAN, AND FIRM; SAME / CENTRILOBULAR,-SLIGHT ON CUT SURFACE; COLLECTED; REGIONAL LN -NECROSIS, COAGULATIVE,-MODERATE (SEE HEPATIC) -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -DIFFUSELY LIGHT; ALL LOBES: TAN -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE -HEMORRHAGE,-PRESENT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : KIDNEY (KD) : -CYST(S); BILATERAL, DIFFUSE: MULTIPLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- CLEAR FLUID FILLED CYSTS, UP TO 3 MM SEVERE IN DIAMETER 2235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 483 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT >NOTE:>UNILATERAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : LUNG (LU) : -DIFFUSELY LIGHT; ALL LOBES: LIGHT RED -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 484 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -MINERALIZATION, VASCULAR,-MODERATE PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, TAN, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MULTIPLE DARK RED PINPOINT FOCI, UP TO MODERATE 1 MM IN DIAMETER -MINERALIZATION, GLANDULAR MUCOSA,- -THICKENED WALL; DIFFUSE MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, LIVER,-PRESENT LN, OTHER (LN) : LN, OTHER (LN) : -DIFFUSELY RED; DARK RED (CAP 22) -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL AORTA (AO) : AORTA (AO) : -LARGE; UP TO 7 MM IN DIAMETER; -MINERALIZATION,-SEVERE COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 485 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 486 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92662 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 431.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:50 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0238 .0055 % .0106 WEIGHT TAKEN RT ADRENAL (AD1) .0252 .0058 % .0112 WEIGHT TAKEN BRAIN (BR) 2.2508 .5217 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.6677 .3866 % .7409 WEIGHT TAKEN RT KIDNEY (KD1) 2.0046 .4647 % .8906 WEIGHT TAKEN LIVER (LI) 14.3620 3.3292 % 6.3808 WEIGHT TAKEN LUNG (LU) 1.9229 .4457 % .8543 WEIGHT TAKEN SPLEEN (SP) .6613 .1533 % .2938 WEIGHT TAKEN LF TESTIS (TE0) 1.6859 .3908 % .7490 WEIGHT TAKEN RT TESTIS (TE1) 1.7014 .3944 % .7559 WEIGHT TAKEN LF THYROID/PARA (TT0) .0084 .0019 % .0037 WEIGHT TAKEN RT THYROID/PARA (TT1) .0146 .0034 % .0065 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 487 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92662 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 431.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:50 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 488 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); SHOULDER-LEFT; WEEK 89; 70 X BRAIN (BR) : BRAIN (BR) : 80MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- -GRANULAR MATERIAL; RIGHT: MULTIPLE MODERATE TAN AND HARD GRANULES, UP TO 1 MM IN -DILATATION, PELVIC,-SLIGHT DIAMETER -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); DISTAL END FROM SPLEEN: -M-CARCINOMA, ISLET CELL,-NEOPLASM SINGLE MASS, 7 MM IN DIAMETER, DARK PRESENT RED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL 2241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 489 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 22 X 13 X 4 -ATROPHY/DEGENERATION,-SEVERE MM; BOTH ARE SOFT -GRANULOMA, SPERM,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, GRAY AND SOFT HARDERIAN GLAND (HG) : -ATROPHY,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC 2242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 490 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT SHOULDER REGION: -M-FIBROSARCOMA,-NEOPLASM PRESENT SINGLE MASS, 80 X 65 X 40 MM, RED, DARK RED, TAN, GRAY, LOBULATED AND FIRM; ON SECTION EXUDES A DARK RED FLUID; PORTION COLLECTED (CAP 25); REGIONAL LN (SEE AXILLARY) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED -CONGESTION,-PRESENT (CAP 24) LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT (CAP 22) -CONGESTION,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MULTIPLE DARK BROWN AND DRY CRUSTED -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT AREAS, UP TO 3 MM IN DIAMETER (CAP 26) -------------------------------------------- 2243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 491 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 492 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92664 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/11/99 8:10 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE MINIMAL >RESPIRATION; LABORED; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 8 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 493 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92664 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/11/99 8:10 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 494 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92665 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/24/99 8:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 10 X 10 MM (RIGHT >UNREMARKABLE IS 5 X 4 X 4 MM) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 495 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92665 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/24/99 8:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -POSSIBLE TRAUMATIC INJURY,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL: UP TO 15 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL DIAMETER; COLLECTED -PROBABLE FRACTURE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 2248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 496 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92665 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/24/99 8:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 497 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92666 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 529.2 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 13:55 PROSECTOR: LYNN HORNER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; ROUGH HAIR COAT; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 498 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92666 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 529.2 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 13:55 PROSECTOR: LYNN HORNER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 499 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92666 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 529.2 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 13:55 PROSECTOR: LYNN HORNER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 500 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92667 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 548.9 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:10 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; EYE (EY) : WEEK 69; 30 X 30MM; VERIFIED AT -DYSTROPHY, CORNEAL IN EARLY STAGES, NECROPSY: NOT OBSERVED CONSISTS PRIMARILY OF FOCAL >EXCRETION; FEW FECES; VERIFIED AT MINERALIZATION,-MINIMAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT ENTIRE; VERIFIED AT NECROPSY: NOT -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; HYPOACTIVE; VERIFIED AT DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-MINIMAL NECROPSY: NOT APPLICABLE SECTIONS -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 501 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92667 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 548.9 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:10 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 7 X 6 X 4 MM (LEFT IS 4 -B-ADENOMA, C-CELL,-NEOPLASM PRESENT X 3 X 2 MM) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT URINARY BLADDER (UB) : URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; FILLED WITH A -HEMORRHAGE,-MODERATE REDDISH-GREEN FLUID -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY MATERIAL -------------------------------------------- 2254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 502 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92667 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 548.9 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:10 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 503 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92668 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:25 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.3505 3.9789 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 504 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92668 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:25 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 505 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92669 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 812.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:18 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT -HYPERPLASIA,-MINIMAL 2258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 506 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92669 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 812.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:18 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGION: -GALACTOCELE,-PRESENT MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 507 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92669 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 812.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:18 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 508 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; PALE; ENTIRE BODY; BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -DEGENERATION, FOCAL,-MINIMAL >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -LARGE; ALL LOBES -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -MASS(ES); RIGHT MEDIAN LOBE, ALONG -VACUOLATION, HEPATOCELLULAR,-SLIGHT NECROPSY: NOT OBSERVED MARGIN: SINGLE MASS, 20 X 20 X 7 MM, -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM >APPEARANCE; HUNCHED; VERIFIED AT RED, TAN, BROWN, AND SOFT; SAME ON CUT PRESENT NECROPSY: NOT APPLICABLE SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NORMAL (CAP 23) -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE SPLEEN (SP) : SPLEEN (SP) : -DIFFUSELY LIGHT; RED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT 2261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 509 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : THYMUS (TH) : -GELATINOUS; CLEAR -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYALINE MATERIAL, ALVEOLAR SEPTA,- MODERATE -HYPERPLASIA, TYPE II PNEUMOCYTE,- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, CRANIAL ONE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- HALF, AT JUNCTION OF NONGLANDULAR MODERATE -MINERALIZATION, GLANDULAR MUCOSA,- MODERATE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 6 X 2 -SECRETION, DECREASED,-PRESENT MM 2262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 510 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 22) -HEMORRHAGE,-PRESENT -MOTTLED; RED AND TAN (CAP 22) LN, OTHER (LN) : >UNREMARKABLE >NOTE:>HEPATIC LN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 511 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 512 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92671 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/00 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/00 7:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 2265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 513 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92671 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/00 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/00 7:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 5 MM IN DIAMETER EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 22) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE -CONGESTION,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL; LOWER INCISORS -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST BROWN FLUID -------------------------------------------- 2266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 514 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92671 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/00 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/00 7:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 515 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK -COMPRESSION, VENTRAL,-PRESENT 95; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >EYES; RED DISCHARGE; EYE-LEFT; -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -VACUOLATION, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED OF MEDIAN LOBES: SINGLE TAN AREA, 5 MM PERIPORTAL,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT IN DIAMETER; COLLECTED ON ROUTINE -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE SECTION PERIPORTAL,-SLIGHT -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 2268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 516 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -LIGHT FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MULTIPLE TAN AREAS, UP TO 2 X 1 MM 2269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 517 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 12 X 8 X 6 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 1 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 23) URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 2270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 518 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 519 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92673 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/00 8:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 81; 50 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC 2272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 520 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92673 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/00 8:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 65 X 50 X 30 MM, RED, >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -------------------------------------------- 2273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 521 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92673 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/00 8:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 522 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92674 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 930.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -ATROPHY, ACINAR,-MODERATE TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; LEFT: CLEAR FLUID -ATROPHY/DEGENERATION,-SEVERE -LARGE; LEFT: 45 X 25 X 20 MM (RIGHT >NOTE:>UNILATERAL IS 20 X 15 X 10 MM) THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 X 5 X 4 MM, RED, TAN, AND -B-ADENOMA,-NEOPLASM PRESENT FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -INFLAMMATION, ACUTE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT 2275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 523 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92674 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 930.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 524 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 90; 15 X 15MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYE-LEFT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE 2277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 525 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT BROWN AND SOFT SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT DORSAL LUMBAR REGION: -CYST, EPIDERMAL INCLUSION,-PRESENT SINGLE MASS, 20 X 15 X 7 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : PROSTATE (PR) : -LARGE; 30 X 20 X 18 MM -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 526 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -GELATINOUS; DIFFUSE: LIGHT GREEN SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: LEFT IS 10 X 7 X 3 -SECRETION, DECREASED,-PRESENT MM, RIGHT IS 15 X 8 X 5 MM -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT BILE DUCT (BD) : BILE DUCT (BD) : -LARGE; UP TO 5 MM IN DIAMETER (CAP -CYST,-PRESENT 23) -INFLAMMATION, CHRONIC,-MODERATELY SEVERE -THROMBUS,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED DRY MATERIAL -------------------------------------------- 2279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 527 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 528 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92676 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0301 .0067 % .0136 WEIGHT TAKEN RT ADRENAL (AD1) .0281 .0063 % .0127 WEIGHT TAKEN BRAIN (BR) 2.2089 .4929 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.8605 .4152 % .8423 WEIGHT TAKEN RT KIDNEY (KD1) 1.8648 .4162 % .8442 WEIGHT TAKEN LIVER (LI) 13.3586 2.9812 % 6.0476 WEIGHT TAKEN LUNG (LU) 1.4477 .3231 % .6554 WEIGHT TAKEN SPLEEN (SP) .6389 .1426 % .2892 WEIGHT TAKEN LF TESTIS (TE0) 1.7039 .3802 % .7714 WEIGHT TAKEN RT TESTIS (TE1) 1.6819 .3753 % .7614 WEIGHT TAKEN LF THYROID/PARA (TT0) .0121 .0027 % .0055 WEIGHT TAKEN RT THYROID/PARA (TT1) .0108 .0024 % .0049 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 529 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92676 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 530 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92677 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/09/00 8:13 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 80; BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION,-MINIMAL LIVER (LI) : LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / ROUNDED EOSINOPHILIC CELL,-MODERATELY SEVERE -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL MULTIPLE TAN AREAS, UP TO 1 MM IN -HYPERTROPHY, HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE PERIPORTAL,-MODERATE SECTIONS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL 2283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 531 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92677 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/09/00 8:13 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -ATROPHY,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -RAISED FOCUS(I)/AREA(S); DORSAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT LUMBAR REGION: SINGLE DRY TAN, CRUSTED RAISED AREA, 10 X 10 X 7 MM (CAP 22) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 532 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92677 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/09/00 8:13 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 533 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92678 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:54 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 10.0762 3.6700 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 534 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92678 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:54 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 535 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92679 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 383 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: 698.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 14:08 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >RESPIRATION; IRREGULAR; VERIFIED AT -HEPATIC ANOMALY (LOBULE); -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE DIAPHRAGMATIC SURFACE AT JUNCTION OF -VACUOLATION, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT MEDIAN LOBES: SINGLE ANOMALY, 8 X 5 X PERIPORTAL,-SLIGHT NECROPSY: NOT APPLICABLE 3 MM, LIVER-LIKE IN CONSISTENCY (CAP -ANOMALY, SECONDARY TO DIAPHRAGMATIC 21) HERNIA,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT 2288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 536 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92679 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 383 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: 698.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 14:08 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,- URETHRA: GREEN AND FIRM MODERATELY SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, AND CRUSTED -------------------------------------------- 2289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 537 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92679 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 383 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: 698.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 14:08 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 538 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92680 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:03 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; PALE; ENTIRE BODY; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / AT NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; HUNCHED; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -ATROPHY, CORTICAL,-MODERATE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 539 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92680 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:03 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 11 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ATROPHY,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 540 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92680 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:03 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 541 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-PROXIMAL; WEEK 62; 20 X BRAIN (BR) : BRAIN (BR) : 10MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE 2294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 542 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, GREY, -B-ADENOMA,-NEOPLASM PRESENT TAN, LOBULATED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN FOCI, UP TO 1 MM IN DIAMETER URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM -LARGE; 31 X 15 X 8 MM SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 2295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 543 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 32 X 21 X 6 MM EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH -INFLAMMATION, CHRONIC-ACTIVE,- OF TAIL: MULTIPLE TAN AND DARK BROWN MODERATELY SEVERE RAISED AREAS, UP TO 16 X 9 X 6 MM; -ACANTHOSIS / HYPERKERATOSIS,-SEVERE POSSIBLY CONSISTENT WITH MASS (TAIL) AS NOTED IN CLINICAL OBSERVATIONS; PORTION COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 544 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 545 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92682 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 887.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:28 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 6 X 3 X 3 MM (LEFT IS 4 -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT X 2 X 2 MM) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, LOBULATED, AND FIRM 2298 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 546 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92682 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 887.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:28 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : HARDERIAN GLAND (HG) : -MOTTLED; BILATERAL: BLACK AND TAN >UNREMARKABLE STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: GREEN, -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT BROWN, AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE HINDFOOT: SINGLE DARK RED, TAN, AND -ULCERATION,-SLIGHT DRY ABRADED AREA, 5 X 3 MM; COLLECTED -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2299 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 547 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92682 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 887.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:28 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2300 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 548 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92683 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 922.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:13 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 6 MM IN -VACUOLATION, HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE PERIPORTAL,-MINIMAL SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,- MODERATE PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2301 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 549 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92683 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 922.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:13 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2302 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 550 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92684 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 427.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0224 .0052 % .0098 WEIGHT TAKEN RT ADRENAL (AD1) .0175 .0041 % .0077 WEIGHT TAKEN BRAIN (BR) 2.2858 .5346 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.8556 .4340 % .8118 WEIGHT TAKEN RT KIDNEY (KD1) 1.8072 .4226 % .7906 WEIGHT TAKEN LIVER (LI) 13.5605 3.1713 % 5.9325 WEIGHT TAKEN LUNG (LU) 2.1432 .5012 % .9376 WEIGHT TAKEN SPLEEN (SP) .6898 .1613 % .3018 WEIGHT TAKEN LF TESTIS (TE0) 1.5608 .3650 % .6828 WEIGHT TAKEN RT TESTIS (TE1) 1.5278 .3573 % .6684 WEIGHT TAKEN LF THYROID/PARA (TT0) .0078 .0018 % .0034 WEIGHT TAKEN RT THYROID/PARA (TT1) .0109 .0025 % .0048 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2303 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 551 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92684 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 427.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2304 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 552 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE OF MEDIAN LOBES: SINGLE TAN AREA, 8 X -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED 5 MM; COLLECTED ON ROUTINE SECTION EOSINOPHILIC CELL,-SLIGHT AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; SQUINTED; PERI-ORBITAL-RIGHT; MINIMAL VERIFIED AT NECROPSY: NOT OBSERVED -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 2305 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 553 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN AND SOFT URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE ORGAN: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SINGLE MASS, 33 X 33 X 23 MM, RED, DARK RED, GREY, TAN, LOBULATED, GELATINOUS AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 28 X 15 X 5 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: UP TO 9 X 7 X 4 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (RIGHT IS UP TO 3 X 2 X 2 MM) (CAP 23) MODERATELY SEVERE 2306 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 554 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL LATERAL CERVICAL -GALACTOCELE,-PRESENT REGIONS: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: RIGHT IS UP TO 7 X -LYMPHANGIECTASIS,-PRESENT 5 X 4 MM, LEFT IS UP TO 6 X 5 X 4 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 24) MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 2307 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 555 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2308 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 556 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92686 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 622.1 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; RED HAIR COAT; VENTRAL- PERIPORTAL,-MODERATELY SEVERE ABDOMINAL; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE 2309 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 557 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92686 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 622.1 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 7 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, GRAY, AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION, EXTENDING TO PERIANAL REGION: BROWN AND DRY -------------------------------------------- 2310 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 558 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92686 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 622.1 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2311 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 559 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92687 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); HEAD-MAXILLARY- BRAIN (BR) : BRAIN (BR) : LEFT; WEEK 57; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >SKIN & PELAGE; ROUGH HAIR COAT; EOSINOPHILIC CELL,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; DILATED PUPIL; EYES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2312 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 560 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92687 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, RED, DARK RED, TAN, LOBULATED AND FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, ACUTE,-MINIMAL MULTIPLE TAN AREAS, UP TO 3 X 2 MM -EROSION,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -ACANTHOSIS / HYPERKERATOSIS,- MULTIPLE TAN AREAS, UP TO 3 X 2 MM MODERATELY SEVERE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); ALONG LOWER LEFT -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL MANDIBLE: SINGLE TAN CRUSTED AREA, 3 X 2 MM (CAP 21) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2313 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 561 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92687 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2314 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 562 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; EYE (EY) : VERIFIED AT NECROPSY: NOT OBSERVED -ATROPHY, RETINAL,-MODERATE >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES: DARK RED AND -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT BROWN -NECROSIS, INDIVIDUAL HEPATOCYTE,- NECROPSY: NOT APPLICABLE MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -CYST(S); BILATERAL, CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- MULTIPLE CLEAR FLUID FILLED CYSTS, SEVERE PINPOINT AND UP TO 1 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL -MOTTLED; BILATERAL: RED, BROWN, AND TAN SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; ALONG DUODENUM: SINGLE -ZYMOGEN GRANULES, DECREASED,-MODERATE VESSEL, 3 MM IN DIAMETER (CAP 24) -HYPERPLASIA, ISLET CELLS,-MODERATELY SEVERE 2315 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 563 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT >NOTE:>SEE ALSO SLIDE #17 ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 5 -VACUOLATION, FOCAL,-SLIGHT X 4 X 3 MM) -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL -DEGENERATION / NECROSIS, UNILATERAL,- SEVERE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT -HYPERPLASIA, MEDULLARY,-SLIGHT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: LEFT IS 2 MM IN -HYPERPLASIA,-MINIMAL DIAMETER, RIGHT IS 4 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, LOBULATED, AND SOFT 2316 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 564 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL MULTIPLE DARK BROWN AREAS UP TO 2 X 1 MM PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL URETHRA: GREEN AND FIRM -LARGE; 30 X 20 X 8 MM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: LEFT IS 35 X 15 X 5 -SECRETION, INCREASED,-PRESENT MM, RIGHT IS 30 X 15 X 3 MM CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 25) MINIMAL -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL, AXILLARY REGIONS: -GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY SEMIFLUID FILLED -B-FIBROADENOMA,-NEOPLASM PRESENT CYSTS, 10 X 5 X 5 MM (CAP 22) LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 23) -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2317 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 565 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2318 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 566 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92689 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/14/99 STUDY DAY OF DEATH: 390 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/14/99 8:26 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2319 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 567 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92689 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/14/99 STUDY DAY OF DEATH: 390 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/14/99 8:26 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -TEETH MISSING/BROKEN/WORN; LEFT LOWER INCISOR: BROKEN; APPROXIMATELY ONE- HALF OF INCISOR REMAINING -------------------------------------------- 2320 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 568 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92689 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/14/99 STUDY DAY OF DEATH: 390 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/14/99 8:26 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2321 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 569 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92690 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1034.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:28 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LEFT; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SEVERE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERPLASIA, ISLET CELLS,-MODERATE TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2322 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 570 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92690 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1034.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:28 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SALIV GL, MANDIB (SG) : SALIV GL, MANDIB (SG) : -MASS(ES); LEFT, ATTACHED TO CRANIAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS END: SINGLE MASS, 12 X 10 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) -DIFFUSELY LIGHT; LEFT: TAN SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT (CAP 23) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 17 X 12 X 6 MM (RIGHT IS -LYMPHANGIECTASIS,-PRESENT 6 X 3 X 2 MM) (CAP 24) -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,- HINDFOOT: SINGLE DRY BROWN ABRADED MODERATELY SEVERE AREA, 4 MM IN DIAMETER; COLLECTED -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2323 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 571 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92690 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1034.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:28 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2324 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 572 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92691 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.0 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; DISCOLORED/LIGHT RED; EYE-RIGHT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); LEFT LATERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 -HYPERTROPHY, HEPATOCELLULAR, X 2 MM; COLLECTED ON ROUTINE SECTION CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -DILATATION / CYST, FOLLICULAR,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2325 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 573 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92691 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.0 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2326 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 574 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92692 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/99 8:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -CONGESTION,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -CONGESTION,-PRESENT HEART (HT) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FIBROSIS,-SLIGHT TRACHEA (TR) : -EXUDATE, LUMINAL,-PRESENT PARATHYROID (PT) : >TISSUE MISSING 2327 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 575 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92692 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/99 8:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GREY AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); SCROTUM: SINGLE RED, -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL DRY CRUSTED AREA, 14 X 10 MM (CAP 21) SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: BOTH ARE 35 X 16 X -SECRETION, INCREASED,-PRESENT 4 MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, CYSTIC,-MODERATE AND FIRM (CAP 22) -SECRETORY MATERIAL IN LUMINA,-PRESENT COAGULATING GL (CG) : COAGULATING GL (CG) : -LARGE; LEFT: 25 X 6 X 4 MM (FOR -SECRETORY MATERIAL, INCREASED,-PRESENT COMPARISON, RIGHT IS 10 X 6 X 3 MM); COLLECTED INTACT WITH SEMINAL VESICLES GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: RED -------------------------------------------- 2328 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 576 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92692 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/99 8:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2329 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 577 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92693 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 711.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 98; 40 X 40MM; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; DORSAL; LOBE NEAR JUNCTION OF LEFT MEDIAN -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED LOBE: SINGLE TAN AREA, 4 MM IN CENTRILOBULAR POLYKARYOCYTOSIS >SKIN & PELAGE; BLACK SKIN; DORSAL; DIAMETER; COLLECTED ON ROUTINE SECTION OCCASIONALLY NOTED,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / MULTIPLE DARK RED AREAS, UP TO 5 MM IN EOSINOPHILIC CELL,-MINIMAL DIAMETER; SOME ARE SUNKEN WITH A -FOCUS, ALTERED HEPATOCELLULAR, STELLATE PATTERN; COLLECTED ON ROUTINE BASOPHILIC,-MINIMAL SECTIONS -HYPERPLASIA, BILE DUCT,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : LUNG (LU) : -MASS(ES); ALL LOBES, DIFFUSE: -N-SARCOMA,-FROM SKIN MULTIPLE MASSES, UP TO 15 MM IN DIAMETER, ALL ARE RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED 2330 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 578 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92693 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 711.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); LUMBAR REGION: SINGLE MASS, -M-FIBROSARCOMA,-NEOPLASM PRESENT 40 X 35 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE ERODED AREA, 15 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 MM IN DIAMETER; -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT APPEARS CYSTIC (CAP 22) -LYMPHANGIECTASIS,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2331 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 579 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92693 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 711.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2332 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 580 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92694 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 918.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE 2333 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 581 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92694 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 918.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -ULCERATION,-SLIGHT SINGLE DARK RED AND DRY CRUSTED AREA, -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2334 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 582 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92694 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 918.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2335 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 583 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92695 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -CYST,-PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2336 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 584 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92695 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TRIGEMINAL NERVE (TG) : TRIGEMINAL NERVE (TG) : -LARGE; BILATERAL: EACH IS UP TO 4 MM -GANGLION,-PRESENT IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), THYROID (TY) ------------------------------------------------------------------------------------------------------------------------------------ 2337 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 585 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92696 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:51 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2338 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 586 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92696 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:51 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR -HYPERPLASIA,-MINIMAL ASPECT: SINGLE TAN AREA, 2 MM IN DIAMETER URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -INFLAMMATION, CHRONIC-ACTIVE,- HINDFOOT: SINGLE DARK RED AND TAN MODERATELY SEVERE ABRADED AREA, 9 MM IN DIAMETER; -ULCERATION,-MODERATE COLLECTED -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -BACTERIA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2339 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 587 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92696 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:51 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2340 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 588 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92697 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/28/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 07/28/99 7:35 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 58; 15 X 15MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; DORSAL- -COMPRESSION, VENTRAL,-PRESENT THORACIC; VERIFIED AT NECROPSY: NOT -MINERALIZATION,-MINIMAL OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >EXCRETION; NO FECES; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-MINIMAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -VACUOLATION, HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE PERIPORTAL,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: OBSERVED SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2341 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 589 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92697 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/28/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 07/28/99 7:35 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, AND SOFT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT DORSAL LUMBAR REGION: >UNREMARKABLE SINGLE MASS, 20 X 15 X 15 MM, RED, >NOTE:>SEE SKIN FOR DIAGNOSIS DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN DRY MATERIAL -------------------------------------------- 2342 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 590 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92697 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/28/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 07/28/99 7:35 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2343 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 591 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92698 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:30 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); AXILLARY REGION- LIVER (LI) : LIVER (LI) : LEFT; WEEK 82; VERIFIED AT NECROPSY: -MASS(ES); ALL LOBES: MULTIPLE MASSES, -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NOT OBSERVED UP 23 X 20 X 8 MM, ALL ARE TAN, BROWN, EOSINOPHILIC CELL,-MINIMAL >SKIN & PELAGE; SORE/SCAB; AXILLARY DARK RED AND FIRM; ALL ARE SAME ON CUT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL REGION-LEFT; VERIFIED AT NECROPSY: NOT SURFACE; COLLECTED ON ROUTINE -HYPERPLASIA, BILE DUCT,-MINIMAL OBSERVED SECTIONS; REGIONAL LN (SEE HEPATIC) -N-CARCINOMA,-FROM PANCREAS >SKIN & PELAGE; BROWN HAIR COAT; KIDNEY (KD) : PERINEAL AREA; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MODERATELY SEVERE >EYES; PALE; EYES; VERIFIED AT PANCREAS (PA) : PANCREAS (PA) : NECROPSY: NOT APPLICABLE -MASS(ES); INVOLVING ENTIRE ORGAN: -M-CARCINOMA, ISLET CELL,-NEOPLASM >SKIN & PELAGE; OTHER; BLACK HAIR COAT - SINGLE MASS, 45 X 40 X 35 MM, DARK RED PRESENT PERINEAL; VERIFIED AT NECROPSY: AND TAN; SAME ON CUT SURFACE; OBSERVED COLLECTED INTACT; REGIONAL LN (SEE >EXCRETION; FEW FECES; VERIFIED AT PANCREATIC) NECROPSY: NOT APPLICABLE TESTIS (TE) : >EXCRETION; LIQUID FECES; VERIFIED AT -MINERALIZATION, VASCULAR,-MINIMAL NECROPSY: NOT APPLICABLE LUNG (LU) : -N-CARCINOMA,-FROM PANCREAS THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL 2344 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 592 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92698 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:30 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 7 X 3 X 3 MM >TISSUE MISSING (CAP 22) LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; PANCREATIC: DARK RED >TISSUE MISSING (CAP 23) >NOTE:>NO LYMPH NODE; SEE PANCREAS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -------------------------------------------- 2345 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 593 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92698 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:30 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2346 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 594 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92699 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 636.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL TESTIS (TE) : TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, -ATROPHY/DEGENERATION,-SEVERE BENEATH TUNICA: SINGLE YELLOW AREA, 11 -B-INTERSTITIAL CELL TUMOR,-NEOPLASM X 10 MM PRESENT >NOTE:>UNILATERAL 2347 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 595 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92699 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 636.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT GREY AND SOFT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE THIRD: -B-KERATOACANTHOMA,-NEOPLASM PRESENT MULTIPLE BROWN AND DRY AREAS, UP TO 2 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2348 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 596 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92699 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 636.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2349 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 597 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92700 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 532.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; VENTRAL- BRAIN (BR) : BRAIN (BR) : ABDOMINAL; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 98; 15 X 15MM; VERIFIED AT NECROPSY: -HYPERTROPHY, HEPATOCELLULAR, NOT OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT -INFLAMMATION, VASCULAR,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT 2350 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 598 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92700 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 532.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT AND SOFT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2351 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 599 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92700 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 532.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) ------------------------------------------------------------------------------------------------------------------------------------ 2352 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 600 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); LIMB-FRONT-RIGHT; WEEK 47; LIVER (LI) : 70 X 80MM; VERIFIED AT NECROPSY: -NECROSIS, INDIVIDUAL HEPATOCYTE,- OBSERVED MINIMAL >SKIN & PELAGE; SORE/SCAB; LIMB-FRONT- -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL RIGHT; VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: OBSERVED PERIPORTAL,-MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; PALE; ENTIRE BODY; MODERATE VERIFIED AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; SPLEEN (SP) : VERIFIED AT NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY, >EYES; RED DISCHARGE; EYE-RIGHT; INCREASED,-MODERATE VERIFIED AT NECROPSY: OBSERVED PANCREAS (PA) : >EXCRETION; FEW FECES; VERIFIED AT -ZYMOGEN GRANULES, DECREASED,-MODERATE NECROPSY: NOT APPLICABLE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE 2353 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 601 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -MASS(ES); SURROUNDING ENTIRE FRONT -M-FIBROSARCOMA,-NEOPLASM PRESENT RIGHT FORELIMB: SINGLE MASS,95 X 80 X -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 45 MM, TAN, RED, FIRM, AND LOBULATED; -BACTERIAL COLONIES,-PRESENT SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DRY RED CRUSTED AREA, 60 X 55 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) ESOPHAGUS (ES) : -HYPERKERATOSIS,-MODERATE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 7 X 4 X 2 -HEMORRHAGE,-PRESENT MM (CAP 21) 2354 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 602 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 2355 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 603 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2356 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 604 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92702 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:10 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SURFACE OF LEFT LATERAL LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / TAN AREA, 2 X 1 MM; COLLECTED ON EOSINOPHILIC CELL,-MODERATELY SEVERE ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 7 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM STOMACH, GL (ST) : -EROSION,-SLIGHT 2357 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 605 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92702 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:10 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2358 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 606 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92702 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:10 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2359 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 607 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92703 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/16/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/16/99 7:48 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 9 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED AND SOFT 2360 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 608 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92703 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/16/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/16/99 7:48 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED -DISCHARGE-NASAL; DARK RED -------------------------------------------- 2361 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 609 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92703 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/16/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/16/99 7:48 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2362 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 610 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92704 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 860.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:35 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 90; 20 X 20MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL LUNG (LU) : LUNG (LU) : >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE AT TRIMMING TRACHEA (TR) : TRACHEA (TR) : >NOTE:>TRIMMING COMMENT: MISSING FROM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL WET TISSUE AT TRIMMING ESOPHAGUS (ES) : ESOPHAGUS (ES) : >NOTE:>TRIMMING COMMENT: MISSING FROM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL WET TISSUE AT TRIMMING 2363 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 611 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92704 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 860.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:35 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : THYROID (TY) : >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE AT TRIMMING PARATHYROID (PT) : PARATHYROID (PT) : >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE AT TRIMMING SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT LATERAL ABDOMINAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 25 MM IN DIAMETER, TAN AND FIRM; ON CUT SURFACE EXUDES A TAN, CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22); OVERLYING SKIN HAS A SINGLE DRY, TAN ULCERATED AREA, 8 MM IN DIAMETER; COLLECTED INTACT WITH MASS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2364 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 612 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92704 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 860.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:35 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2365 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 613 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92705 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 852.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:23 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; DISCOLORED/LIGHT RED; EYE-RIGHT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); LEFT MEDIAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL LOBE: MULTIPLE TAN FOCI, UP TO 2 MM IN -HYPERTROPHY, HEPATOCELLULAR, DIAMETER; COLLECTED CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2366 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 614 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92705 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 852.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:23 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -LARGE; LEFT: 6 X 4 X 3 MM (RIGHT IS 4 X 3 X 2 MM) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT LATERAL THORACIC -B-FIBROADENOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 20 X 15 X 7 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2367 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 615 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92705 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 852.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:23 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2368 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 616 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92706 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 789.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -M-ASTROCYTOMA,-NEOPLASM PRESENT >APPEARANCE; LIMITED USE; LIMBS-HIND; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT APPLICABLE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 2369 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 617 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92706 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 789.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 6 MM, RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT BROWN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL OBESE -DISCHARGE-ORAL; CLEAR -------------------------------------------- 2370 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 618 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92706 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 789.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2371 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 619 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >DISCHARGE; NASAL; RED IN COLOR; -MOTTLED; ALL LOBES: TAN AND BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED PERIPORTAL,-MODERATELY SEVERE AT NECROPSY: NOT APPLICABLE -CYST, BILIARY,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -LARGE PELVIS(ES); BILATERAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -LIGHT FOCUS(I)/AREA(S); RIGHT -BACTERIAL COLONIES,-PRESENT CORTICAL SURFACE: MULTIPLE TAN AREAS, UP TO 1 MM IN DIAMETER SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 2372 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 620 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 11 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- TAN ERODED AREAS, UP TO 3 MM IN MODERATELY SEVERE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK RED AND TAN -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 23) MODERATE -HEMORRHAGE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -INFLAMMATION / NECROSIS, FAT,-SLIGHT CRANIAL TO LEFT TESTIS: SINGLE MASS, 6 X 5 X 5 MM, YELLOW AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE LUMBAR) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2373 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 621 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -DISCHARGE-NASAL; DARK RED AND DRY -URINARY BLADDER-LARGE LUMEN; 25 X 20 X 20 MM; CONTAINS A GREEN FLUID -------------------------------------------- 2374 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 622 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2375 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 623 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92708 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 724.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -ECTOPIC HEPATOCYTES,-PRESENT TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS 2376 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 624 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92708 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 724.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2377 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 625 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; PALE; ENTIRE BODY; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -NECROSIS, COAGULATIVE,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); TRIMMING COMMENT: BILATERAL, -NEPHROPATHY, CHRONIC PROGRESSIVE,- DIFFUSE: MULTIPLE CLEAR FLUID FILLED SEVERE CYSTS, UP TO 1 MM IN DIAMETER -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 6 MM IN -INFLAMMATION, VASCULAR,-SEVERE DIAMETER (CAP 23) ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: LEFT IS 16 X 11 X 5 -ATROPHY/DEGENERATION,-SLIGHT MM, RIGHT IS 20 X 12 X 6 MM; BOTH ARE -INFLAMMATION, VASCULAR,-SLIGHT SOFT 2378 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 626 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, SEPTAL,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-MODERATELY SEVERE PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, TAN, GRAY, -B-ADENOMA,-NEOPLASM PRESENT AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -MINERALIZATION, GLANDULAR MUCOSA,- MODERATELY SEVERE CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2379 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 627 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 6 X 2 -ATROPHY,-SEVERE MM EPIDIDYMIDES (EP) : -ATROPHY/DEGENERATION,-MODERATE -VACUOLATION, TUBULAR EPITHELIUM,- MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 6 MM IN -MINERALIZATION,-SLIGHT DIAMETER; COLLECTED GENERAL COMMENT (GC) : -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY -------------------------------------------- 2380 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 628 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2381 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 629 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92710 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/18/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/18/99 15:15 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MINIMAL -ATROPHY, ACINAR,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2382 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 630 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92710 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/18/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/18/99 15:15 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2383 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 631 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92710 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/18/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/18/99 15:15 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2384 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 632 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92711 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 8:04 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : >TISSUE MISSING HEART (HT) : -CARDIOMYOPATHY,-SLIGHT ESOPHAGUS (ES) : -EXUDATE, LUMINAL,-PRESENT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT 2385 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 633 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92711 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 8:04 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -FIBROSIS,-SLIGHT SINGLE TAN SLIGHTLY RAISED AREA, 8 X 5 MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 30 X 20 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2386 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 634 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92711 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 8:04 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2387 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 635 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92712 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE LOBE: SINGLE TAN AREA, 5 X 3 MM; PERIPORTAL,-SLIGHT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED COLLECTED ON ROUTINE SECTION -NECROSIS, COAGULATIVE,-SLIGHT AT NECROPSY: NOT APPLICABLE >NOTE:>TRIMMING COMMENT: RIGHT MEDIAN -INFILTRATE, MACROPHAGE, PIGMENTED,- LOBE OF LIVER MISSING AT SLIGHT TRIMMING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL 2388 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 636 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92712 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN AND SOFT PROSTATE (PR) : PROSTATE (PR) : -GELATINOUS; CLEAR AND SOFT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: UP TO 4 X 4 X 3 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (RIGHT IS UP TO 3 X 3 X 2 MM) (CAP 22) MODERATE -DIFFUSELY RED; LEFT -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT 2389 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 637 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92712 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2390 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 638 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92713 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0305 .0056 % .0134 WEIGHT TAKEN RT ADRENAL (AD1) .0304 .0056 % .0133 WEIGHT TAKEN BRAIN (BR) 2.2845 .4226 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.8515 .3425 % .8105 WEIGHT TAKEN RT KIDNEY (KD1) 1.8679 .3455 % .8176 WEIGHT TAKEN LIVER (LI) 18.6941 3.4580 % 8.1830 WEIGHT TAKEN LUNG (LU) 2.0157 .3729 % .8823 WEIGHT TAKEN SPLEEN (SP) .6982 .1292 % .3056 WEIGHT TAKEN LF TESTIS (TE0) 1.6468 .3046 % .7209 WEIGHT TAKEN RT TESTIS (TE1) 1.6167 .2991 % .7077 WEIGHT TAKEN LF THYROID/PARA (TT0) .0130 .0024 % .0057 WEIGHT TAKEN RT THYROID/PARA (TT1) .0112 .0021 % .0049 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -HYPERPLASIA, ISLET CELLS,-SLIGHT 2391 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 639 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92713 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2392 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 640 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92714 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:29 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0325 .0061 % .0131 WEIGHT TAKEN RT ADRENAL (AD1) .0386 .0072 % .0155 WEIGHT TAKEN BRAIN (BR) 2.4852 .4656 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.3377 .4379 % .9406 WEIGHT TAKEN RT KIDNEY (KD1) 2.2096 .4139 % .8891 WEIGHT TAKEN LIVER (LI) 20.0992 3.7653 % 8.0876 WEIGHT TAKEN LUNG (LU) 5.3343 .9993 % 2.1464 WEIGHT TAKEN SPLEEN (SP) .6615 .1239 % .2662 WEIGHT TAKEN LF TESTIS (TE0) 1.8935 .3547 % .7619 WEIGHT TAKEN RT TESTIS (TE1) 1.9044 .3568 % .7663 WEIGHT TAKEN LF THYROID/PARA (TT0) .0132 .0025 % .0053 WEIGHT TAKEN RT THYROID/PARA (TT1) .0217 .0041 % .0087 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL 2393 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 641 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92714 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:29 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2394 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 642 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92715 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:06 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.7462 3.6290 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2395 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 643 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92715 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:06 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2396 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 644 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92716 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 429.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:40 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; DISCOLORED/LIGHT RED; EYES; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >APPEARANCE; THIN; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: NOT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >EXCRETION; NONFORMED FECES; VERIFIED BASOPHILIC,-MINIMAL AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL -CYST,-PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 7 -ATROPHY/DEGENERATION,-SEVERE MM; BOTH ARE SOFT THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT -DEPLETION, LYMPHOCYTIC (GENERALLY POSITIVELY IDENTIFIED, POSSIBLE COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- REMNANTS COLLECTED MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL URINARY BLADDER (UB) : URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; DISTAL END: GREEN AND FIRM -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2397 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 645 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92716 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 429.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:40 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -HYPERPLASIA, EPITHELIAL,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -CYST(S); SINGLE CLEAR FLUID FILLED -LYMPHANGIECTASIS,-MODERATE CYST, 3 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2398 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 646 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92716 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 429.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:40 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2399 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 647 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92717 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:44 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK LIVER (LI) : 86; 30 X 30MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >MASS(ES); DORSAL; WEEK 98; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT NECROPSY: NOT OBSERVED -FIBROSIS, PERIBILIARY,-SLIGHT >SKIN & PELAGE; SORE/SCAB; DORSAL; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -CYST(S); BILATERAL CORTICAL SURFACES: -NEPHROPATHY, CHRONIC PROGRESSIVE,- SINGLE CLEAR FLUID FILLED CYST ON SEVERE EACH, LEFT IS 1 MM IN DIAMETER, RIGHT -CYST,-PRESENT IS 2 MM IN DIAMETER -THROMBUS,-PRESENT -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; TRIMMING COMMENT: BILATERAL: -DILATATION / CYST, FOLLICULAR,-PRESENT EACH IS 10 X 6 X 3 MM PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE 2400 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 648 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92717 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:44 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT, AND RIGHT POSTERIOR ASPECT: -HYPERPLASIA,-SLIGHT SINGLE DARK RED AREA ON EACH, BOTH ARE 2 MM IN DIAMETER SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT LATERAL THORACIC >NOT REQUIRED TO BE EXAMINED FOR ANIMAL REGION: SINGLE MASS, 35 X 30 X 20 MM, >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS TAN, FIRM, AND VASCULAR; SAME ON CUT SURFACE; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2401 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 649 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92717 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:44 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2402 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 650 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92718 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 278.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:00 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.1215 3.2752 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2403 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 651 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92718 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 278.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:00 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2404 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 652 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92719 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 461.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:00 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0301 .0065 % .0134 WEIGHT TAKEN RT ADRENAL (AD1) .0255 .0055 % .0113 WEIGHT TAKEN BRAIN (BR) 2.2522 .4883 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.9914 .4318 % .8842 WEIGHT TAKEN RT KIDNEY (KD1) 2.0458 .4436 % .9084 WEIGHT TAKEN LIVER (LI) 15.8391 3.4343 % 7.0327 WEIGHT TAKEN LUNG (LU) 1.7406 .3774 % .7728 WEIGHT TAKEN SPLEEN (SP) .6376 .1382 % .2831 WEIGHT TAKEN LF TESTIS (TE0) 1.6425 .3561 % .7293 WEIGHT TAKEN RT TESTIS (TE1) 1.6706 .3622 % .7418 WEIGHT TAKEN LF THYROID/PARA (TT0) .0220 .0048 % .0098 WEIGHT TAKEN RT THYROID/PARA (TT1) .0225 .0049 % .0100 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DILATATION, PELVIC,-SLIGHT -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2405 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 653 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92719 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 461.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:00 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2406 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 654 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92720 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 13:21 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 76; LIVER (LI) : 140 X 130MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- >SKIN & PELAGE; SORE/SCAB; VENTRAL- MINIMAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, OBSERVED PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE 2407 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 655 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92720 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 13:21 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT ABDOMINAL REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 150 X 140 X 50 MM, RED, >NOTE:>SEE SKIN FOR DIAGNOSIS DARK RED, TAN, VASCULAR, AND FIRM; SOME AREAS EXUDE A DARK BROWN FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN, DRY, CRUSTED AREA, 90 X 65 MM; COLLECTED WITH PORTION OF MASS CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -INFLAMMATION / NECROSIS, FAT,-SLIGHT SURROUNDING RIGHT TESTIS: SINGLE MASS, 13 X 5 X 4 MM, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 22); REGIONAL LN (LUMBAR) NOT IDENTIFIED -------------------------------------------- 2408 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 656 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92720 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 13:21 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2409 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 657 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92721 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:30 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED LIVER (LI) : AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-MODERATE >EXCRETION; NONFORMED FECES; VERIFIED KIDNEY (KD) : KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -PIGMENT, INCREASED,-MODERATE AT NECROPSY: NOT APPLICABLE PANCREAS (PA) : PANCREAS (PA) : >EYES; SQUINTED; PERI-ORBITAL-RIGHT; -DIFFUSELY RED; RED -ZYMOGEN GRANULES, DECREASED,- VERIFIED AT NECROPSY: NOT APPLICABLE MODERATELY SEVERE >SKIN & PELAGE; RED HAIR COAT; HEAD- -INFILTRATE, MACROPHAGE, PIGMENTED,- ENTIRE; VERIFIED AT NECROPSY: NOT MINIMAL OBSERVED -ATROPHY, ACINAR,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2410 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 658 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92721 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:30 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 12 X 6 X 6 MM, DARK RED, TAN, SOFT, AND LOBULATED HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, ACUTE,-SLIGHT MULTIPLE PINPOINT DARK BROWN AREAS -THICKENED WALL; MUCOSA: DIFFUSE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE DARK BROWN ERODED AREA, 4 MM IN -ULCERATION,-MODERATE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -THICKENED WALL; MUCOSA: DIFFUSE -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE PINPOINT DARK BROWN AREAS URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE 2411 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 659 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92721 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:30 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2412 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 660 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92722 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:30 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0278 .0055 % .0125 WEIGHT TAKEN RT ADRENAL (AD1) .0245 .0049 % .0110 WEIGHT TAKEN BRAIN (BR) 2.2279 .4414 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.7612 .3490 % .7905 WEIGHT TAKEN RT KIDNEY (KD1) 1.7928 .3552 % .8047 WEIGHT TAKEN LIVER (LI) 16.2488 3.2195 % 7.2933 WEIGHT TAKEN LUNG (LU) 2.2034 .4366 % .9890 WEIGHT TAKEN SPLEEN (SP) .6357 .1260 % .2853 WEIGHT TAKEN LF TESTIS (TE0) 1.5641 .3099 % .7021 WEIGHT TAKEN RT TESTIS (TE1) 1.5630 .3097 % .7016 WEIGHT TAKEN LF THYROID/PARA (TT0) .0102 .0020 % .0046 WEIGHT TAKEN RT THYROID/PARA (TT1) .0107 .0021 % .0048 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : >NOTE:>TRIMMING COMMENT: MESENTERIC LN >TISSUE MISSING MISSING FROM WET TISSUE AT TIME OF TRIMMING 2413 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 661 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92722 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:30 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2414 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 662 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92723 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 552 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 685.3 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 9:58 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; SQUINTED; PERI-ORBITAL-RIGHT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; RED HAIR COAT; VENTRAL- PERIPORTAL,-MODERATE ABDOMINAL; VERIFIED AT NECROPSY: -HYPERPLASIA, BILE DUCT,-SLIGHT OBSERVED KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2415 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 663 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92723 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 552 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 685.3 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 9:58 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 8 X 5 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT SALIV GL, MANDIB (SG) : -HYPERPLASIA, GLANDULAR,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE REDDISH-TAN ERODED AREAS, UP TO 4 X 2 -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MM -THICKENED WALL; MUCOSA: DIFFUSE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 13 X 5 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED 2416 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 664 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92723 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 552 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 685.3 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 9:58 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE OF ANIMAL: DARK RED AND DRY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2417 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 665 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92724 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 975.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:12 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 73; LIVER (LI) : 10 X 10MM; VERIFIED AT NECROPSY: NOT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >WART LIKE LESION(S); DORSAL; WEEK 96; EOSINOPHILIC CELL,-SLIGHT VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; SORE/SCAB; VENTRAL- BASOPHILIC,-SLIGHT ABDOMINAL; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID VENTRAL -B-KERATOACANTHOMA,- 2 NEOPLASMS ABDOMINAL AREA: SINGLE TAN, RED, DARK PRESENT RED AND DRY CRUSTED AREA, 10 X 8 MM; (CAP 22) 2418 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 666 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92724 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 975.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:12 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -RAISED FOCUS(I)/AREA(S); MID DORSAL THORACIC REGION: TWO YELLOW, DARK RED AND DRY AREAS, UP TO 9 X 3 X 2 MM (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2419 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 667 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92724 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 975.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:12 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2420 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 668 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92725 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/99 STUDY DAY OF DEATH: 363 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/99 9:08 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >RESPIRATION; AUDIBLE; VERIFIED AT BRAIN (BR) : NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL >RESPIRATION; LABORED; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-MINIMAL >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / AT NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -PIGMENT, INCREASED,-MINIMAL MAXILLARY-LEFT; VERIFIED AT NECROPSY: ADRENAL, MEDULLA (MA) : OBSERVED -HYPERPLASIA, MEDULLARY,-MINIMAL >SKIN & PELAGE; RED HAIR COAT; HEAD- LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : MAXILLARY-RIGHT; VERIFIED AT NECROPSY: -LARGE; UP TO 35 X 22 X 18 MM -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT OBSERVED -DIFFUSELY RED; DARK RED THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -CONGESTION,-PRESENT 2421 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 669 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92725 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/99 STUDY DAY OF DEATH: 363 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/99 9:08 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -HEMORRHAGE,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT ESOPHAGUS (ES) : -EXUDATE, LUMINAL,-PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); PYLORIC MUCOSA: -EDEMA,-MODERATE SINGLE AREA, 2 X 1 MM -INFLAMMATION, ACUTE,-MINIMAL -HEMORRHAGE,-MINIMAL URINARY BLADDER (UB) : URINARY BLADDER (UB) : -DIFFUSELY RED; DARK RED -HEMORRHAGE,-SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, ABDOMINAL CAVITY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -DISCHARGE-NASAL; DARK RED, DRY, CRUSTED MATERIAL; CONSISTENT WITH RED HAIR COAT (BILATERAL, MAXILLARY) AS NOTED IN CLINICAL OBSERVATIONS -------------------------------------------- 2422 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 670 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92725 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/99 STUDY DAY OF DEATH: 363 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/99 9:08 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2423 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 671 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92726 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:38 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 10 X 6 X 4 MM (LEFT IS -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM 7 X 3 X 2 MM) PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 4 X 3 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2424 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 672 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92726 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:38 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2425 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 673 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- PELVIS: MULTIPLE TAN GRANULES, UP TO 2 SLIGHT MM IN DIAMETER -MINERALIZATION, PELVIC,-MODERATELY -ROUGH SURFACE, DIFFUSE; BILATERAL SEVERE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 18 X 13 X 2 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE 18 X 13 X 8 MM); RIGHT IS SOFT >NOTE:>UNILATERAL 2426 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 674 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FIBROSIS,-MINIMAL -MINERALIZATION, ALVEOLAR LUMINA,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 5 MM, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2427 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 675 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 8 X 1 -ATROPHY,-SEVERE MM CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE DRY AREA, 5 MM IN DIAMETER; MODERATELY SEVERE COLLECTED GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: UP TO 10 MM IN DIAMETER -------------------------------------------- 2428 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 676 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2429 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 677 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92728 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 580.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:39 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0439 .0076 % .0207 WEIGHT TAKEN RT ADRENAL (AD1) .0398 .0069 % .0188 WEIGHT TAKEN BRAIN (BR) 2.1202 .3650 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.3132 .3982 % 1.0910 WEIGHT TAKEN RT KIDNEY (KD1) 2.3640 .4070 % 1.1150 WEIGHT TAKEN LIVER (LI) 23.2456 4.0017 % 10.9639 WEIGHT TAKEN LUNG (LU) 2.0336 .3501 % .9592 WEIGHT TAKEN SPLEEN (SP) .8902 .1532 % .4199 WEIGHT TAKEN LF TESTIS (TE0) 1.5272 .2629 % .7203 WEIGHT TAKEN RT TESTIS (TE1) 1.5527 .2673 % .7323 WEIGHT TAKEN LF THYROID/PARA (TT0) .0132 .0023 % .0062 WEIGHT TAKEN RT THYROID/PARA (TT1) .0133 .0023 % .0063 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED >NOT REQUIRED TO BE EXAMINED FOR ANIMAL (CAP 21) 2430 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 678 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92728 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 580.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:39 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2431 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 679 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92729 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 532.7 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:55 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 77; 20 X 20MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, >EXCRETION; NO FECES; VERIFIED AT PERIPORTAL,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: OBSERVED MINIMAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -HYALINE DROPLETS, TUBULAR EPITHELIUM,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : >UNREMARKABLE 2432 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 680 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92729 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 532.7 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:55 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 6 MM; EACH IS SOFT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : PROSTATE (PR) : -SMALL; 15 X 10 X 5 MM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 10 X 5 X 2 -SECRETION, DECREASED,-PRESENT MM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT, INVOLVING ENTIRE -LYMPHANGIECTASIS,-PRESENT NODE: SINGLE CLEAR FLUID FILLED CYST, 12 X 7 X 6 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY MATERIAL -DISCHARGE-NASAL; RED AND DRY MATERIAL -------------------------------------------- 2433 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 681 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92729 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 532.7 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:55 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2434 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 682 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92730 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 547.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:21 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 105; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; ATAXIC; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE PINPOINT DARK RED AREAS; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / COLLECTED ON ROUTINE SECTIONS EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT -B-ADENOMA, ISLET CELL,- 2 NEOPLASMS PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2435 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 683 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92730 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 547.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:21 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT MAXILLARY REGION NEAR -ULCERATION,-MODERATE COMMISSURE: SINGLE MASS, 6 X 4 X 2 MM, -FIBROSIS,-MODERATE GRAY AND FIRM (CAP 22); REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2436 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 684 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92730 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 547.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:21 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2437 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 685 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92731 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 321.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:13 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.3081 3.5232 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2438 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 686 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92731 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 321.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:13 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2439 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 687 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92732 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:28 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT -------------------------------------------- 2440 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 688 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92732 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:28 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2441 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 689 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92733 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 411.6 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >SKIN & PELAGE; RED HAIR COAT; LATERAL- OCCASIONALLY NOTED,-MINIMAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE 2442 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 690 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92733 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 411.6 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MULTIPLE THICKENED >UNREMARKABLE AREAS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; RIGHT LATERAL HEAD AND RIGHT LATERAL THORACIC REGIONS: DARK RED AND DRY -------------------------------------------- 2443 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 691 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92733 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 411.6 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2444 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 692 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92734 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:08 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.9230 3.1183 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2445 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 693 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92734 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:08 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2446 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 694 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92735 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 804.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 4 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-MODERATELY SEVERE SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MODERATE THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 5 X 3 -DILATATION / CYST, FOLLICULAR,-PRESENT MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2447 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 695 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92735 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 804.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2448 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 696 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92736 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 867.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 MM IN DIAMETER (LEFT -PIGMENT,-SLIGHT IS 5 X 4 X 4 MM) 2449 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 697 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92736 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 867.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 19 X 11 X 6 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE 26 X 15 X 11 MM); RIGHT APPEARS SOFT -INFLAMMATION, VASCULAR,-SLIGHT >NOTE:>UNILATERAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ANTERIOR >NOT REQUIRED TO BE EXAMINED FOR ANIMAL ASPECT: SINGLE FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-PRESENT COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 2450 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 698 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92736 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 867.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2451 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 699 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 78; 10 X 10MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; CONVULSIONS; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -DEGENERATION, CYSTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE 2452 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 700 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 9 X 9 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 2 MM IN -ULCERATION,-MINIMAL DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,- -THICKENED WALL; DIFFUSE MODERATELY SEVERE SKIN (SK) : SKIN (SK) : -RAISED FOCUS(I)/AREA(S); MID DORSAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT LUMBAR REGION: SINGLE TAN AND DRY AREA, 7 X 6 X 5 MM (CAP 22); POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2453 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 701 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DARK RED AND DRY -------------------------------------------- 2454 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 702 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2455 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 703 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92738 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/20/00 7:58 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 8 X 6 MM (LEFT IS -B-ADENOMA,-NEOPLASM PRESENT 5 X 4 X 3 MM) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -M-MALIGNANT INTERSTITIAL CELL TUMOR,- NEOPLASM PRESENT 2456 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 704 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92738 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/20/00 7:58 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERTROPHY, FOLLICULAR CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GREY, TAN AND FIRM JEJUNUM (JE) : >AUTOLYTIC URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE -INFILTRATE, LYMPHOHISTIOCYTIC,- MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -------------------------------------------- 2457 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 705 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92738 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/20/00 7:58 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2458 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 706 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92739 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL -THROMBUS,-PRESENT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2459 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 707 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92739 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,- MODERATE -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE PROSTATE (PR) : PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE PROSTATE: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SINGLE MASS, 25 X 20 X 14 MM, GREEN, TAN, FIRM, LOBULATED, AND GELATINOUS; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 25 X 10 X -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 10 MM; BOTH ARE FIRM -MOTTLED; BILATERAL: TAN AND GREEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 2 X 6 X 3 -LYMPHANGIECTASIS,-PRESENT MM, RIGHT IS 10 X 6 X 3 MM (CAP 22) -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2460 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 708 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92739 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2461 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 709 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92740 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 855.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 20 X 13 X 9 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE 26 X 16 X 11 MM); RIGHT APPEARS SOFT >NOTE:>UNILATERAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT 2462 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 710 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92740 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 855.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 11 X 9 X 5 MM (LEFT IS 6 X 3 X 2 MM); RIGHT APPEARS CYSTIC (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2463 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 711 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >APPEARANCE; HUNCHED; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-SLIGHT >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -GRANULAR MATERIAL; RIGHT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED PELVIS: MULTIPLE TAN AND FIRM MINIMAL AT NECROPSY: NOT APPLICABLE GRANULES, UP TO 1 MM IN DIAMETER -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -ATROPHY, CORTICAL,-MODERATE TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 11 X 3 -ATROPHY/DEGENERATION,-SEVERE MM; BOTH ARE SOFT 2464 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 712 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- TAN ERODED AREAS, UP TO 3 MM IN MODERATELY SEVERE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE -HYPERPLASIA, TRANSITIONAL CELL,-SEVERE TAN AND FIRM CALCULI, UP TO 9 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE DIAMETER -DIFFUSELY RED; MUCOSA: DARK RED -THICKENED WALL; DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 10 X 2 -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MM EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- SLIGHT 2465 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 713 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS UP TO 20 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 10 X 10 MM, RIGHT IS UP TO 12 X 8 X 4 -DILATATION, SINUSOIDAL,-SEVERE MM (CAP 22) TAIL (TI) : TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH: -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MULTIPLE DARK RED AND TAN AREAS, UP TO -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT 5 X 4 X 4 MM; COLLECTED FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SINGLE DARK RED, TAN AND DRY AREA ON -ULCERATION,-MODERATE EACH, RIGHT IS 10 X 4 X 4 MM, LEFT IS -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 10 X 10 X 7 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -URINARY BLADDER-LARGE LUMEN; 30 X 20 X 20 MM -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: RED AND MOIST -------------------------------------------- 2466 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 714 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2467 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 715 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92742 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 66; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- MULTIPLE DARK RED AREAS, UP TO 4 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / RIGHT; VERIFIED AT NECROPSY: OBSERVED DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-SLIGHT SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE -ECTASIA, TUBULAR,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 4 X 2 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT 2468 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 716 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92742 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL CERVICAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE, YELLOW, DARK RED, DRY AREA, 23 X 20 MM; COLLECTED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SINGLE RED, TAN, AND DRY AREA, 6 X 4 -ACANTHOSIS / HYPERKERATOSIS,-MODERATE MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2469 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 717 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92742 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2470 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 718 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92743 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/27/99 14:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; OBES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT DUODENUM (DU) : >AUTOLYTIC 2471 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 719 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92743 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/27/99 14:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 2472 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 720 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92743 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/27/99 14:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2473 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 721 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92744 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 302.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:11 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.7687 3.8962 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2474 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 722 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92744 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 302.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:11 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2475 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 723 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >MASS(ES); INGUINAL REGION-RIGHT; WEEK CONSISTS PRIMARILY OF FOCAL 84; 10 X 10MM; VERIFIED AT NECROPSY: MINERALIZATION,-MODERATE NOT OBSERVED BRAIN (BR) : BRAIN (BR) : >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; PALE; ENTIRE BODY; -DIFFUSELY LIGHT; ALL LOBES: TAN -VACUOLATION, HEPATOCELLULAR, MIDZONAL VERIFIED AT NECROPSY: NOT APPLICABLE / CENTRILOBULAR,-MODERATELY SEVERE >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NECROSIS, COAGULATIVE,-MINIMAL NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MODERATE 2476 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 724 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATE TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 18 X 10 X 3 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE 20 X 13 X 6 MM); BOTH ARE SOFT >NOTE:>UNILATERAL BONE, FEMUR (FE) : -HYPERPLASIA, BONE,-MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : >SECTION EXAMINED; TISSUE NOT PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-MODERATE -HYPERPLASIA, BONE,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : LUNG (LU) : -RED FOCUS(I)/AREA(S); VENTRAL RIGHT -HEMORRHAGE,-PRESENT CAUDAL LOBE: SINGLE DARK RED AREA, 3 MM IN DIAMETER HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-MODERATE 2477 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 725 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 6 X 5 MM, DARK BROWN, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL MULTIPLE DARK BROWN AREAS, UP TO 2 MM -MINERALIZATION, GLANDULAR MUCOSA,- IN DIAMETER MODERATE -THICKENED WALL; DIFFUSE SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE MOIST, RED AND BROWN CRUSTED AREA, 10 MM IN DIAMETER (CAP 22) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: 18 X 5 X 2 MM -ATROPHY,-MODERATELY SEVERE -GELATINOUS; BILATERAL: TAN CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-MODERATELY SEVERE 2478 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 726 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL: SINGLE MOIST AND BROWN ULCERATED AREA ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 12 MM IN DIAMETER; BOTH ARE COLLECTED AORTA (AO) : AORTA (AO) : -FIRM; AORTIC ARCH, DIFFUSE (CAP 23) -MINERALIZATION,-SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2479 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 727 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2480 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 728 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92746 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 749.0 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: DARK RED AND TAN -CONGESTION,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2481 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 729 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92746 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 749.0 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 6 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -INFLAMMATION, ACUTE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- RED AND TAN ERODED AREAS, UP TO 6 X 3 MODERATELY SEVERE MM -ULCERATION,-MODERATE -THICKENED WALL; DIFFUSE: APPEARS -INFLAMMATION, CHRONIC-ACTIVE,- GELATINOUS MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2482 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 730 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92746 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 749.0 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2483 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 731 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92747 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 716.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:38 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; SWOLLEN; PAW-FRONT-RIGHT; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS >EYES; RED DISCHARGE; EYE-RIGHT; OCCASIONALLY NOTED,-MINIMAL VERIFIED AT NECROPSY: NOT OBSERVED -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 22) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 2484 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 732 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92747 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 716.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:38 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 10 X 6 X 6 MM (LEFT IS -LYMPHANGIECTASIS,-PRESENT 2 MM IN DIAMETER) (CAP 23) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT CARPAL AND RIGHT TARSAL -INFLAMMATION, CHRONIC-ACTIVE,- JOINT: RIGHT TARSAL IS 20 X 13 X 11 MM MODERATELY SEVERE (LEFT IS 16 X 8 X 7 MM), RIGHT CARPAL IS 14 X 10 X 8 MM (LEFT IS 10 X 6 X 6 MM); BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2485 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 733 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92747 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 716.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:38 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2486 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 734 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92748 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:49 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -LARGE; BILATERAL: EACH IS 30 X 23 X SEVERE 12 MM -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; DIFFUSE: UP TO 5 MM IN -HYPERPLASIA, LYMPHOPLASMACYTIC,- DIAMETER MODERATE -INFLAMMATION, VASCULAR,-SEVERE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2487 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 735 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92748 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:49 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 7 X 5 -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT X 3 MM (CAP 22) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 11 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 8 X 5 MM (CAP 23) CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -LARGE VESSELS; INVOLVING ENTIRE -INFLAMMATION, VASCULAR,-SEVERE MESENTERY: UP TO 2 MM IN DIAMETER (CAP -THROMBUS,-PRESENT 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2488 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 736 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92748 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:49 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2489 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 737 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92749 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 9:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 18 X 14 X 10 MM (LEFT -ATROPHY/DEGENERATION,-SEVERE IS 25 X 17 X 14 MM); RIGHT IS SOFT >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC 2490 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 738 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92749 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 9:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 2491 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 739 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92749 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 9:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2492 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 740 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92750 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 660.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:33 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, ALONG -EROSION,-MODERATE GREATER CURVATURE: SINGLE RED, ERODED -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT AREA, 5 MM IN DIAMETER 2493 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 741 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92750 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 660.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:33 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2494 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume VII of IX Page 2495 of 4068 Covance 6329-183 3M T-6295 VOLUME VII OF IX CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 2497 Individual Anatomic Pathology Data ..................................................................... 2498 2496 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 2497 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 742 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92751 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 801.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:39 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0273 .0034 % .0118 WEIGHT TAKEN RT ADRENAL (AD1) .0281 .0035 % .0121 WEIGHT TAKEN BRAIN (BR) 2.3191 .2893 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.4820 .3097 % 1.0702 WEIGHT TAKEN RT KIDNEY (KD1) 2.4333 .3036 % 1.0492 WEIGHT TAKEN LIVER (LI) 32.0284 3.9961 % 13.8107 WEIGHT TAKEN LUNG (LU) 2.3737 .2962 % 1.0235 WEIGHT TAKEN SPLEEN (SP) .9244 .1153 % .3986 WEIGHT TAKEN LF TESTIS (TE0) 1.5886 .1982 % .6850 WEIGHT TAKEN RT TESTIS (TE1) 1.6268 .2030 % .7015 WEIGHT TAKEN LF THYROID/PARA (TT0) .0240 .0030 % .0103 WEIGHT TAKEN RT THYROID/PARA (TT1) .0264 .0033 % .0114 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERTROPHY, HEPATOCELLULAR,- MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 2498 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 743 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92751 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 801.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:39 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA >UNREMARKABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA >UNREMARKABLE RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2499 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 744 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92751 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 801.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:39 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2500 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 745 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92752 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.7254 4.2670 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2501 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 746 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92752 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2502 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 747 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92753 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 733.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:51 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-MODERATE -THROMBUS,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 2503 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 748 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92753 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 733.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:51 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 6 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, AND SOFT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN AT BASE -ANIMAL OBESE -------------------------------------------- 2504 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 749 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92753 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 733.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:51 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2505 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 750 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92754 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0265 .0048 % .0109 WEIGHT TAKEN RT ADRENAL (AD1) .0263 .0047 % .0108 WEIGHT TAKEN BRAIN (BR) 2.4325 .4392 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.1434 .3870 % .8812 WEIGHT TAKEN RT KIDNEY (KD1) 2.1609 .3902 % .8883 WEIGHT TAKEN LIVER (LI) 20.2188 3.6509 % 8.3119 WEIGHT TAKEN LUNG (LU) 2.1405 .3865 % .8800 WEIGHT TAKEN SPLEEN (SP) .7109 .1284 % .2923 WEIGHT TAKEN LF TESTIS (TE0) 1.5214 .2747 % .6254 WEIGHT TAKEN RT TESTIS (TE1) 1.5586 .2814 % .6407 WEIGHT TAKEN LF THYROID/PARA (TT0) .0153 .0028 % .0063 WEIGHT TAKEN RT THYROID/PARA (TT1) .0164 .0030 % .0067 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL 2506 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 751 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92754 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2507 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 752 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92754 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2508 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 753 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92755 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 695.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:55 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0201 .0029 % .0089 WEIGHT TAKEN RT ADRENAL (AD1) .0226 .0033 % .0100 WEIGHT TAKEN BRAIN (BR) 2.2492 .3235 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.3890 .3436 % 1.0622 WEIGHT TAKEN RT KIDNEY (KD1) 2.4440 .3515 % 1.0866 WEIGHT TAKEN LIVER (LI) 28.2494 4.0629 % 12.5598 WEIGHT TAKEN LUNG (LU) 1.8793 .2703 % .8355 WEIGHT TAKEN SPLEEN (SP) .8195 .1179 % .3644 WEIGHT TAKEN LF TESTIS (TE0) 1.7651 .2539 % .7848 WEIGHT TAKEN RT TESTIS (TE1) 1.8308 .2633 % .8140 WEIGHT TAKEN LF THYROID/PARA (TT0) .0200 .0029 % .0089 WEIGHT TAKEN RT THYROID/PARA (TT1) .0170 .0024 % .0076 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2509 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 754 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92755 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 695.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:55 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -HEMORRHAGE,-PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: -CONGESTION,-PRESENT MULTIPLE DARK RED AREAS, UP TO 3 X 1 MM EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2510 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 755 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92755 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 695.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:55 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2511 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 756 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 84; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; DORSAL; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 2512 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 757 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 5 X 4 MM -ULCERATION,-MODERATE -THICKENED WALL; DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE TAN, DRY, AND RAISED AREA, 7 X 7 X 6 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS AND SCAB NOTED IN CLINICAL OBSERVATIONS 2513 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 758 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2514 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 759 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2515 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 760 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); DORSAL; WEEK 98; 10 X 10MM; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE 2516 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 761 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC -CYST, EPIDERMAL INCLUSION,-PRESENT REGION: SINGLE MASS, 15 X 15 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS MATERIAL; COLLECTED; REGIONAL LN (LEFT AXILLARY) NORMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2517 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 762 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS UP TO 35 X -SECRETION, INCREASED,-PRESENT 20 X 6 MM EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 2518 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 763 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2519 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 764 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92758 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 15:05 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >BEHAVIOR; HYPOACTIVE; VERIFIED AT OCCASIONALLY NOTED,-SLIGHT NECROPSY: NOT APPLICABLE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL 2520 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 765 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92758 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 15:05 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, TAN, RED, -B-ADENOMA,-NEOPLASM PRESENT BROWN AND FIRM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2521 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 766 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92758 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 15:05 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2522 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 767 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92759 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:22 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 15.7536 4.9187 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2523 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 768 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92759 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:22 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2524 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 769 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92760 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.5 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 11:20 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED MULTIPLE DARK RED AREAS, UP TO 4 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-MINIMAL SECTIONS -ANGIECTASIS,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -MOTTLED; BILATERAL: BROWN AND DARK -NEPHROPATHY, CHRONIC PROGRESSIVE,- BROWN MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, BROWN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, ACUTE,-MINIMAL TAN ERODED AREA, 2 MM IN DIAMETER -EROSION,-MINIMAL 2525 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 770 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92760 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.5 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 11:20 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -------------------------------------------- 2526 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 771 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92760 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.5 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 11:20 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2527 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 772 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92761 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2528 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 773 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92761 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2529 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 774 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92761 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2530 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 775 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -MASS(ES); LEFT LATERAL LOBE: SINGLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MASS, 40 X 30 X 15 MM, DARK RED, -HYPERTROPHY, HEPATOCELLULAR, BROWN, TAN, AND SOFT; SAME ON CUT CENTRILOBULAR POLYKARYOCYTOSIS SURFACE; COLLECTED ON ROUTINE SECTION; OCCASIONALLY NOTED,-SLIGHT REGIONAL LN (HEPATIC) NORMAL (CAP 22) -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL 2531 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 776 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: LEFT IS 17 X 13 X 4 -ATROPHY/DEGENERATION,-SEVERE MM, RIGHT IS 15 X 11 X 4 MM; BOTH ARE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT SOFT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- DARK BROWN LINEAR AREA, 3 X 1 MM SLIGHT 2532 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 777 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >TISSUE MISSING >NOTE:>HEPATIC GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2533 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 778 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2534 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 779 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAWS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: DARK RED AND TAN -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; YELLOW HAIR COAT; PERIPORTAL,-SLIGHT VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; BROWN HAIR COAT; -DIFFUSELY DARK; BILATERAL: DARK BROWN MODERATELY SEVERE PERINEAL AREA; VERIFIED AT NECROPSY: SPLEEN (SP) : OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE 2535 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 780 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -B-ADENOMA, BRONCHIOLAR-ALVEOLAR,- NEOPLASM PRESENT MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-SLIGHT MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MM MODERATELY SEVERE -THICKENED WALL; DIFFUSE SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS UP TO 13 X -SECRETION, DECREASED,-PRESENT 6 X 2 MM EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT 2536 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 781 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 45 X 4 X 3 -ATROPHY/DEGENERATION,-SLIGHT MM CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; BILATERAL TARSAL JOINTS: EACH -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE IS UP TO 13 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERINEUM: BROWN, MOIST MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: YELLOW AND MOIST -------------------------------------------- 2537 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 782 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2538 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 783 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92764 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 393.7 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >BEHAVIOR; ATAXIC; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE SURFACE OF RIGHT MEDIAN LOBE: SINGLE -DEGENERATION, CYSTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT TAN AREA, 2 X 1 MM; COLLECTED ON -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT APPLICABLE ROUTINE SECTION -FIBROSIS, PERIBILIARY,-SLIGHT >APPEARANCE; HUNCHED; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE SLIGHT >BEHAVIOR; COLD TO TOUCH; LIMBS-ALL; -VACUOLATION, HEPATOCELLULAR,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; COLD TO TOUCH; TAIL; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT 2539 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 784 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92764 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 393.7 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 10 X 10 -B-ADENOMA,-NEOPLASM PRESENT MM, RED, DARK RED, TAN AND FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, ALONG >UNREMARKABLE GREATER CURVATURE: SINGLE BLACK ERODED AREA, 2 X 1 MM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2540 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 785 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92764 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 393.7 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2541 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 786 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92765 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 493.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:52 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0267 .0054 % .0119 WEIGHT TAKEN RT ADRENAL (AD1) .0260 .0053 % .0116 WEIGHT TAKEN BRAIN (BR) 2.2505 .4562 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.6982 .3443 % .7546 WEIGHT TAKEN RT KIDNEY (KD1) 1.6461 .3337 % .7314 WEIGHT TAKEN LIVER (LI) 18.5015 3.7506 % 8.2211 WEIGHT TAKEN LUNG (LU) 1.8585 .3767 % .8258 WEIGHT TAKEN SPLEEN (SP) .8329 .1688 % .3701 WEIGHT TAKEN LF TESTIS (TE0) 1.6630 .3371 % .7389 WEIGHT TAKEN RT TESTIS (TE1) 1.7142 .3475 % .7617 WEIGHT TAKEN LF THYROID/PARA (TT0) .0113 .0023 % .0050 WEIGHT TAKEN RT THYROID/PARA (TT1) .0118 .0024 % .0052 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -DILATATION, PELVIC,-MODERATELY SEVERE -MINERALIZATION, PELVIC,-SLIGHT >NOTE:>RENAL LESIONS ARE UNILATERAL. SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2542 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 787 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92765 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 493.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:52 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2543 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 788 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); RECTUM; WEEK 99; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-MINIMAL SPINAL CORD (SC) : -MINERALIZATION, DURA,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT 2544 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 789 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -RAISED FOCUS(I)/AREA(S); OVERLYING -ANGIECTASIS WITH THROMBOSIS AND RIGHT TESTIS: SINGLE RED, RAISED AREA, ENDOTHELIAL PROLIFERATION,-PRESENT 4 MM IN DIAMETER (CAP 22); POSSIBLY CONSISTENT WITH MASS (RECTUM) NOTED IN CLINICAL OBSERVATIONS EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2545 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 790 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY MATERIAL -------------------------------------------- 2546 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 791 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2547 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 792 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; LATERAL- LIVER (LI) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- >APPEARANCE; OBES; VERIFIED AT MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -NEPHROPATHY, CHRONIC PROGRESSIVE,- ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: SLIGHT OBSERVED SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MODERATE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MODERATE 2548 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 793 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -METAPLASIA, OSSEOUS / CARTILAGINOUS,- MINIMAL DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATELY SEVERE 2549 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 794 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,- LATERAL THORACIC REGION: SINGLE MOIST MODERATELY SEVERE RED ULCERATED AREA, 35 X 25 MM; -ULCERATION,-MODERATE COLLECTED -BACTERIAL COLONIES,-PRESENT -CRUSTED AREA(S); RIGHT INGUINAL REGION: SINGLE DRY BROWN CRUSTED AREA, 10 MM IN DIAMETER (CAP 22) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 15 X 11 X 10 MM (RIGHT >UNREMARKABLE IS 7 X 4 X 2 MM)(CAP 24) GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 2550 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 795 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2551 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 796 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 85; 80 X 100MM; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HYPERTROPHY, HEPATOCELLULAR, ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED OCCASIONALLY NOTED,-SLIGHT >SKIN & PELAGE; BLACK SKIN; DORSAL; -VACUOLATION, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT OBSERVED PERIPORTAL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -HYALINE DROPLETS, TUBULAR EPITHELIUM,- MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 18 X 12 X 4 MM (RIGHT IS -ATROPHY/DEGENERATION,-SEVERE 20 X 14 X 8 MM); LEFT IS SOFT >NOTE:>UNILATERAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2552 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 797 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NERVE, SCIATIC (SN) : >TISSUE MISSING THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 1 MM IN -HYPERPLASIA,-SLIGHT DIAMETER SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT VENTRAL -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL ABDOMINAL REGION: SINGLE TAN AND DRY -M-FIBROSARCOMA,-NEOPLASM PRESENT CRUSTED AREA, 1 MM IN DIAMETER (CAP 22) -MASS(ES); DORSAL LUMBAR REGION: SINGLE MASS, 100 X 85 X 60 MM, RED, DARK RED, TAN AND FIRM; SOME SECTIONS EXUDE A TAN FLUID (CAP 23); OVERLYING SKIN HAS A SINGLE DARK RED, TAN AND DRY CRUSTED AREA, 70 X 60 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2553 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 798 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2554 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 799 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2555 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 800 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; PAWS-ALL; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-MODERATELY SEVERE -MINERALIZATION, PELVIC,-MINIMAL -NECROSIS, PAPILLARY,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL 2556 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 801 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : -ATROPHY,-SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); ATTACHED TO LEFT ASPECT: 5 -B-ADENOMA,-NEOPLASM PRESENT MM IN DIAMETER, RED, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE GREY AREAS, UP TO 2 X 1 MM JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2557 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 802 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- 2558 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 803 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2559 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 804 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92770 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 7:26 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); PAW-FRONT-LEFT; WEEK 97; LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; SWOLLEN; LIMBS-HIND; -GRANULAR CYTOPLASM, EOSINOPHILIC, VERIFIED AT NECROPSY: OBSERVED CENTRILOBULAR,-MINIMAL >APPEARANCE; LIMITED USE; LIMBS-ALL; -HYPERPLASIA, BILE DUCT,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; YELLOW HAIR COAT; SLIGHT VERIFIED AT NECROPSY: OBSERVED SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 5 X 5 MM (LEFT IS 5 >UNREMARKABLE X 3 X 3 MM) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATELY SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-MODERATELY SEVERE 2560 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 805 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92770 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 7:26 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -CYST,-PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE HINDFOOT: SINGLE RED, TAN MOIST ABRADED AREA, 4 X 3 MM; COLLECTED; POSSIBLY CONSISTENT WITH (MASS LEFT HINDFOOT) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: RED, BROWN AND MOIST -STAINS-PERINEUM/PERIANAL; YELLOW, BROWN AND MOIST -------------------------------------------- 2561 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 806 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92770 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 7:26 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2562 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 807 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92771 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 567 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 684.7 GRAMS DATE AND TIME OF NECROPSY: 11/07/99 7:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; VENTRAL- -COMPRESSION, VENTRAL,-PRESENT ABDOMINAL; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -LARGE; ALL LOBES -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; RED HAIR COAT; HEAD- PERIPORTAL,-SEVERE ENTIRE; VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, TUBULAR EPITHELIUM,- >EXCRETION; NO FECES; VERIFIED AT MODERATELY SEVERE NECROPSY: NOT APPLICABLE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2563 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 808 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92771 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 567 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 684.7 GRAMS DATE AND TIME OF NECROPSY: 11/07/99 7:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERTROPHY, FOLLICULAR CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE, EXTENDING TO ENTIRE HEAD: DRY, RED MATERIAL -------------------------------------------- 2564 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 809 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92771 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 567 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 684.7 GRAMS DATE AND TIME OF NECROPSY: 11/07/99 7:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2565 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 810 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92772 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 360 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: 670.3 GRAMS DATE AND TIME OF NECROPSY: 04/14/99 10:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES: MARGINS ARE ROUNDED -HYPERTROPHY, HEPATOCELLULAR, -MOTTLED; ALL LOBES: RED AND TAN CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -CONGESTION,-PRESENT -VACUOLATION, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -MOTTLED; BILATERAL: BROWN AND TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -PIGMENT, TUBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2566 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 811 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92772 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 360 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: 670.3 GRAMS DATE AND TIME OF NECROPSY: 04/14/99 10:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2567 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 812 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; VENTRAL-ABDOMINAL- LIVER (LI) : LIVER (LI) : LEFT; VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED, BROWN AND -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / TAN EOSINOPHILIC CELL,-MODERATELY SEVERE -MASS(ES); LEFT LATERAL LOBE AND RIGHT -FOCUS, ALTERED HEPATOCELLULAR, LATERAL LOBES: TWO MASSES, BOTH ARE 20 BASOPHILIC,-MODERATE X 20 X 10 MM AND TAN, RED AND FIRM; -DEGENERATION, CYSTIC,-SLIGHT SAME ON CUT SURFACE; LEFT LATERAL -HYPERPLASIA, BILE DUCT,-SLIGHT COLLECTED ON ROUTINE SECTION, RIGHT -FIBROSIS, PERIBILIARY,-SLIGHT LATERAL COLLECTED AS EXTRA SECTION; -HYPERTROPHY, HEPATOCELLULAR, REGIONAL LN (HEPATIC) NORMAL (CAP 22) PERIPORTAL,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 2568 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 813 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 15 X 10 X 6 MM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -DIFFUSELY RED; DARK RED -FIBROSIS,-MODERATE -ANGIECTASIS,-MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE -CYST,-PRESENT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : >NOTE:>TRIMMING COMMENT: PITUITARY NOT >TISSUE MISSING FOUND AT TRIMMING HARDERIAN GLAND (HG) : -DYSPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : -HEMORRHAGE,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE 2569 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 814 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : CECUM (CE) : -MASS(ES); MID PORTION OF CECUM: -BACTERIA,-PRESENT SINGLE MASS, 65 X 50 X 35 MM, RED, -ULCERATION,-MODERATELY SEVERE DARK RED, TAN, FIRM, AND VASCULAR; -B-LIPOMA,-NEOPLASM PRESENT SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MESENTERIC); POSSIBLY CONSISTENT WITH SWOLLEN (ABDOMINAL- VENTRAL-LEFT) AS NOTED IN CLINICAL OBSERVATIONS COLON (CO) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, ABDOMINAL CAVITY,-PRESENT LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT >NOTE:>HEPATIC LN LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK RED AND TAN -LYMPHANGIECTASIS,-PRESENT (CAP 25) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL -HEMORRHAGE,-PRESENT CAVITY, ABDOM (PC) : -HEMATOMA,-PRESENT 2570 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 815 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE NEAR HEAD OF RIGHT EPIDIDYMIS: SINGLE MASS, 23 X 13 X 5 MM, RED, DARK RED, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE LUMBAR) GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; CECUM AND COLON: DISTENDED UP TO 13 MM; FILLED WITH A DARK RED FLUID -FLUID-ABDOMINAL CAVITY; FILLED WITH A RED FLUID -GI TRACT-DARK FLUID CONTENT; JEJUNUM, ILEUM, CECUM AND COLON: FILLED WITH A DARK RED FLUID -------------------------------------------- 2571 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 816 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2572 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 817 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92774 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 767.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 2573 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 818 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92774 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 767.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MUSCLE, SKELETAL (SM) : -DEGENERATION,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE >UNREMARKABLE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2574 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 819 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92774 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 767.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2575 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 820 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 72; 10 X 10MM; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >SKIN & PELAGE; SORE/SCAB; DORSAL; CONSISTS PRIMARILY OF FOCAL VERIFIED AT NECROPSY: OBSERVED MINERALIZATION,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : NECROPSY: NOT APPLICABLE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -FOCUS, ALTERED HEPATOCELLULAR, SURFACE OF LEFT LATERAL LOBE: SINGLE BASOPHILIC,-MODERATE TAN FOCUS, 2 MM IN DIAMETER (CAP 22) -DEGENERATION, CYSTIC,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -LARGE; BILATERAL: EACH IS 30 X 20 X SEVERE 15 MM SPLEEN (SP) : SPLEEN (SP) : -LARGE; 60 X 16 X 8 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2576 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 821 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 6 MM (RIGHT IS -VACUOLATION, FOCAL,-MINIMAL 6 X 5 X 5 MM) -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 20 X 11 X 7 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE 21 X 15 X 11 MM) >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT 2577 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 822 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATELY SEVERE THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 4 X 3 -HYPERPLASIA, C-CELL,-MINIMAL MM -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 3 X 2 X 1 -HYPERPLASIA,-MODERATELY SEVERE MM PITUITARY (PI) : PITUITARY (PI) : -CYST(S); LEFT ANTERIOR ASPECT: SINGLE -B-ADENOMA,-NEOPLASM PRESENT CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE -MINERALIZATION, GLANDULAR MUCOSA,- MODERATELY SEVERE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT -EDEMA,-SEVERE 2578 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 823 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -MASS #2; LEFT LATERAL THORACIC REGION: SINGLE MASS, 15 X 15 X 8 MM, TAN AND FIRM; CUT SURFACE EXUDES A TAN CASEOUS SEMISOLID; COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 5 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 25) -SUBCUTANEOUS EDEMA; VENTRAL CERVICAL REGION: CLEAR AND GELATINOUS (CAP 23) PROSTATE (PR) : PROSTATE (PR) : -GELATINOUS; DISTAL PORTION: CLEAR -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT -VACUOLATION, TUBULAR EPITHELIUM,- SLIGHT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: 8 X 6 X 4 MM (RIGHT IS 5 -HEMORRHAGE,-PRESENT X 4 X 2 MM) (CAP 24) -DILATATION, SINUSOIDAL,-SEVERE -DIFFUSELY RED; BILATERAL: DARK RED (CAP 24) 2579 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 824 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 6 MM IN -MINERALIZATION,-MODERATE DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2580 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 825 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); MOUTH; WEEK 102; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE TAN, SLIGHTLY RAISED AREAS, -HYPERTROPHY, HEPATOCELLULAR, UP TO 8 MM IN DIAMETER; COLLECTED ON CENTRILOBULAR POLYKARYOCYTOSIS ROUTINE SECTIONS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : KIDNEY (KD) : -MASS(ES); LEFT, CORTICAL SURFACE -NEPHROPATHY, CHRONIC PROGRESSIVE,- EXTENDING INTO MEDULLA: SINGLE MASS, MINIMAL 10 MM IN DIAMETER, TAN AND FIRM; SAME -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT ON CUT SURFACE; COLLECTED INTACT; -B-LIPOMA,-NEOPLASM PRESENT REGIONAL LN (SEE RENAL) 2581 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 826 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT MAXILLARY REGION: >UNREMARKABLE SINGLE MASS, 4 X 2 X 2 MM, TAN AND FIRM (CAP 22); REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT 2582 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 827 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; LEFT: 6 MM IN DIAMETER, RIGHT >NOT REQUIRED TO BE EXAMINED FOR ANIMAL IS 3 X 2 X 1 MM (CAP 25) >NOTE:>NO LYMPH NODE PRESENT MASSES APPEAR TO BE PART OF THE KIDNEY NEOPLASM LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 25 X 25 X 10 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2583 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 828 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2584 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 829 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92777 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 425.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:15 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0202 .0047 % .0109 WEIGHT TAKEN RT ADRENAL (AD1) .0180 .0042 % .0097 WEIGHT TAKEN BRAIN (BR) 1.8515 .4352 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.0608 .4844 % 1.1130 WEIGHT TAKEN RT KIDNEY (KD1) 2.1126 .4966 % 1.1410 WEIGHT TAKEN LIVER (LI) 20.8174 4.8936 % 11.2435 WEIGHT TAKEN LUNG (LU) 1.5024 .3532 % .8115 WEIGHT TAKEN SPLEEN (SP) .6408 .1506 % .3461 WEIGHT TAKEN LF TESTIS (TE0) 1.5350 .3608 % .8291 WEIGHT TAKEN RT TESTIS (TE1) 1.5449 .3632 % .8344 WEIGHT TAKEN LF THYROID/PARA (TT0) .0136 .0032 % .0073 WEIGHT TAKEN RT THYROID/PARA (TT1) .0106 .0025 % .0057 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL 2585 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 830 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92777 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 425.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:15 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2586 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 831 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92778 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0286 .0042 % .0121 WEIGHT TAKEN RT ADRENAL (AD1) .0286 .0042 % .0121 WEIGHT TAKEN BRAIN (BR) 2.3638 .3494 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.7978 .4136 % 1.1836 WEIGHT TAKEN RT KIDNEY (KD1) 2.6827 .3966 % 1.1349 WEIGHT TAKEN LIVER (LI) 30.0439 4.4411 % 12.7100 WEIGHT TAKEN LUNG (LU) 2.2742 .3362 % .9621 WEIGHT TAKEN SPLEEN (SP) .9348 .1382 % .3955 WEIGHT TAKEN LF TESTIS (TE0) 1.0030 .1483 % .4243 WEIGHT TAKEN RT TESTIS (TE1) 1.0456 .1546 % .4423 WEIGHT TAKEN LF THYROID/PARA (TT0) .0242 .0036 % .0102 WEIGHT TAKEN RT THYROID/PARA (TT1) .0422 .0062 % .0179 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL 2587 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 832 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92778 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 13 X 8 >UNREMARKABLE MM; BOTH ARE SOFT EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2588 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 833 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92778 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2589 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 834 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92779 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:36 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 11.8757 4.3268 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2590 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 835 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92779 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:36 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2591 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 836 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92780 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 515.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT SURFACE OF RIGHT MEDIAN LOBE: SINGLE -HYPERTROPHY, HEPATOCELLULAR, TAN AREA, 5 X 3 MM; COLLECTED ON CENTRILOBULAR POLYKARYOCYTOSIS ROUTINE SECTION OCCASIONALLY NOTED,-MODERATE -ROUGH SURFACE, DIFFUSE; ALL LOBES -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MODERATE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL 2592 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 837 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92780 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 515.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -PIGMENT,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND SOFT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL URETHRA: GREEN AND FIRM 2593 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 838 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92780 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 515.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: YELLOW AND >UNREMARKABLE CLEAR CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2594 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 839 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92781 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 14:08 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; PALE; ENTIRE BODY; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; OCCASIONALLY NOTED,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE -PIGMENT, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT CENTRILOBULAR,-MINIMAL NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >APPEARANCE; TREMORS; ENTIRE BODY; MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -SMALL; 30 X 8 X 44 MM -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MODERATE -ATROPHY, CORTICAL,-MODERATELY SEVERE TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 9 X 5 -ATROPHY/DEGENERATION,-SEVERE MM; BOTH ARE SOFT 2595 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 840 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92781 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 14:08 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 6 MM, TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -ATROPHY,-MODERATE STOMACH, GL (ST) : -EROSION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE PROSTATE (PR) : PROSTATE (PR) : -SMALL; 15 X 8 X 4 MM -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE -ATROPHY,-MODERATELY SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 7 X 2 -ATROPHY,-MODERATELY SEVERE MM EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : -SMALL; BILATERAL: BOTH ARE 40 X 5 X 4 -ATROPHY/DEGENERATION,-MODERATELY MM SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2596 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 841 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92781 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 14:08 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2597 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 842 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92782 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 972.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:57 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 103; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERTROPHY, HEPATOCELLULAR, ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: MULTIPLE DARK RED AREAS, UP TO 4 X 3 CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED MM; SOME ARE SUNKEN WITH A STELLATE OCCASIONALLY NOTED,-MINIMAL PATTERN; COLLECTED ON ROUTINE SECTIONS -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL 2598 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 843 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92782 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 972.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:57 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, TAN AND FIRM -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; ALL REGIONS: DIFFUSE >UNREMARKABLE RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID VENTRAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE ABDOMINAL REGION: SINGLE DRY, RED -SUPERFICIAL CRUSTING,-SLIGHT CRUSTED AREA, 4 X 2 MM (CAP 22) -FIBROSIS,-MODERATE URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 844 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92782 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 972.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:57 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 25 X 18 X 15 MM -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 845 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -INFLAMMATION, ACUTE,-MINIMAL >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -HEMORRHAGE,-MINIMAL NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >RESPIRATION; AUDIBLE; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NECROPSY: NOT APPLICABLE MULTIPLE TAN, SLIGHTLY RAISED AREAS, NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT >RESPIRATION; LABORED; VERIFIED AT UP TO 20 X 12 MM; COLLECTED ON ROUTINE NECROPSY: NOT APPLICABLE SECTIONS >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED SLIGHT AT NECROPSY: NOT APPLICABLE -HYALINE DROPLETS, TUBULAR EPITHELIUM,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : TESTIS (TE) : -SMALL; RIGHT: 20 X 10 X 4 MM (LEFT IS -ATROPHY/DEGENERATION,-SEVERE 24 X 12 X 10 MM) >NOTE:>UNILATERAL 2601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 846 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -DARK FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MM -MINERALIZATION, VASCULAR,-MINIMAL -HEMORRHAGE,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-MODERATE MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 -EROSION,-MINIMAL MM -MINERALIZATION, GLANDULAR MUCOSA,- -RED FOCUS(I)/AREA(S); MUCOSA: MINIMAL MULTIPLE AREAS, UP TO 7 X 4 MM -FIBROSIS,-MODERATE CECUM (CE) : CECUM (CE) : -RED FOCUS(I)/AREA(S); MUCOSA: -EROSION,-SLIGHT MULTIPLE DARK RED AREAS, UP TO 13 X 6 -HEMORRHAGE,-SLIGHT MM PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT 2602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 847 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, MOIST MATERIAL -------------------------------------------- 2603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 848 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 849 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92784 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 8:28 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NONFORMED FECES; VERIFIED EYE (EY) : AT NECROPSY: NOT APPLICABLE -DYSTROPHY, CORNEAL IN EARLY STAGES, >EXCRETION; FEW FECES; VERIFIED AT CONSISTS PRIMARILY OF FOCAL NECROPSY: NOT APPLICABLE MINERALIZATION,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL -GLYCOGEN, HEPATOCELLULAR, INCREASED,- SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-MINIMAL 2605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 850 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92784 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 8:28 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 9 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT SOFT, AND LOBULATED STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL TAN ERODED AREAS, UP TO 3 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT DIAMETER URINARY BLADDER (UB) : -HEMORRHAGE,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 851 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92784 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 8:28 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 852 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-SLIGHT -MOTTLED; BILATERAL: RED AND TAN -MINERALIZATION, TUBULAR,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL SPINAL CORD (SC) : >UNREMARKABLE >NOTE:>2 SECTIONS EXAMINED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 853 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -CYST(S); LEFT ASPECT: SINGLE CYST, 4 -B-ADENOMA,-NEOPLASM PRESENT MM IN DIAMETER, FILLED WITH A DARK RED FLUID STOMACH, GL (ST) : -MINERALIZATION, VASCULAR,-MINIMAL URINARY BLADDER (UB) : URINARY BLADDER (UB) : -RED FOCUS(I)/AREA(S); MUCOSA: >AUTOLYTIC MULTIPLE DARK RED AREAS, UP TO 4 X 6 MM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 22) -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL 2609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 854 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DRY RED MATERIAL -URINARY BLADDER-LARGE LUMEN; 22 X 22 X 40 MM, FILLED WITH A DARK RED FLUID -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY BROWN MATERIAL -------------------------------------------- 2610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 855 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 856 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92786 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 829.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:16 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE TAN AREAS, UP TO 4 MM IN -HYPERTROPHY, HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE CENTRILOBULAR POLYKARYOCYTOSIS SECTIONS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT 2612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 857 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92786 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 829.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:16 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -MASS(ES); ALONG DUODENUM: SINGLE MASS, 16 X 12 X 8 MM, RED, DARK RED, TAN, YELLOW, FIRM, AND LOBULATED; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (ANTERIOR MESENTERIC) NORMAL (CAP 23) ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- DARK BROWN AREA, 4 X 2 MM SLIGHT -THICKENED WALL; DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE 2613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 858 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92786 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 829.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:16 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, ANT MES/PANC (AP) ------------------------------------------------------------------------------------------------------------------------------------ 2614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 859 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92787 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 328 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; LIMBS-HIND; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -DEGENERATION / NECROSIS, MIDZONAL,- MODERATE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-SLIGHT -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT SPINAL CORD (SC) : -HEMORRHAGE,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -CONGESTION,-PRESENT 2615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 860 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92787 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 328 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); MID -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL ABDOMINAL REGION: SINGLE RED AND TAN -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT ULCERATED AREA, 10 MM IN DIAMETER (CAP -ULCERATION,-MODERATE 21) URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE >AUTOLYTIC TAN AND FIRM CALCULI, UP TO 4 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; RIGHT: 8 X 5 X 2 MM (FOR -HYPERPLASIA, LYMPHOPLASMACYTIC,- COMPARISON LEFT IS 4 X 2 X 1 MM) MODERATE 2616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 861 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92787 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 328 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 54 X 33 X 30 MM, FILLED WITH A DARK RED FLUID -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 862 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92788 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HYPERTROPHY, HEPATOCELLULAR, MULTIPLE TAN AREAS, PINPOINT AND UP TO CENTRILOBULAR POLYKARYOCYTOSIS 2 X 1 MM; COLLECTED ON ROUTINE OCCASIONALLY NOTED,-MODERATELY SEVERE SECTIONS -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 2618 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 863 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92788 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT TESTIS (TE) : TESTIS (TE) : -LARGE; RIGHT: 27 X 17 X 15 MM (LEFT -ATROPHY/DEGENERATION,-SEVERE IS 21 X 15 X 11 MM); RIGHT IS SOFT >NOTE:>UNILATERAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALONG CAUDAL >UNREMARKABLE MARGIN OF LEFT LOBE: SINGLE TAN AREA, 4 X 3 MM HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2619 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 864 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92788 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2620 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 865 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92789 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 429.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:12 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0241 .0056 % .0109 WEIGHT TAKEN RT ADRENAL (AD1) .0290 .0068 % .0131 WEIGHT TAKEN BRAIN (BR) 2.2120 .5153 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.9168 .4465 % .8665 WEIGHT TAKEN RT KIDNEY (KD1) 1.9639 .4575 % .8878 WEIGHT TAKEN LIVER (LI) 17.7112 4.1256 % 8.0069 WEIGHT TAKEN LUNG (LU) 1.7041 .3969 % .7704 WEIGHT TAKEN SPLEEN (SP) .7950 .1852 % .3594 WEIGHT TAKEN LF TESTIS (TE0) 1.5051 .3506 % .6804 WEIGHT TAKEN RT TESTIS (TE1) 1.6074 .3744 % .7267 WEIGHT TAKEN LF THYROID/PARA (TT0) .0108 .0025 % .0049 WEIGHT TAKEN RT THYROID/PARA (TT1) .0112 .0026 % .0051 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MULTIPLE DARK RED PINPOINT FOCI 2621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 866 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92789 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 429.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:12 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 867 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92790 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 608.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0252 .0041 % .0112 WEIGHT TAKEN RT ADRENAL (AD1) .0215 .0035 % .0095 WEIGHT TAKEN BRAIN (BR) 2.2573 .3708 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.6769 .4398 % 1.1859 WEIGHT TAKEN RT KIDNEY (KD1) 2.5370 .4168 % 1.1239 WEIGHT TAKEN LIVER (LI) 30.5157 5.0133 % 13.5187 WEIGHT TAKEN LUNG (LU) 1.8934 .3111 % .8388 WEIGHT TAKEN SPLEEN (SP) .7560 .1242 % .3349 WEIGHT TAKEN LF TESTIS (TE0) 1.5265 .2508 % .6763 WEIGHT TAKEN RT TESTIS (TE1) 1.5153 .2489 % .6713 WEIGHT TAKEN LF THYROID/PARA (TT0) .0212 .0035 % .0094 WEIGHT TAKEN RT THYROID/PARA (TT1) .0294 .0048 % .0130 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -PIGMENT, TUBULAR,-MINIMAL -HYALINE DROPLETS, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 2623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 868 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92790 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 608.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL RECTUM (RE) : -PARASITISM,-PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN 2624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 869 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92790 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 608.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 870 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92791 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 826.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:12 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 93; 20 X 20MM; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; DORSAL; SURFACE OF RIGHT MEDIAN LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: OBSERVED TAN AREA, 3 MM IN DIAMETER; COLLECTED EOSINOPHILIC CELL,-MODERATE ON ROUTINE SECTION -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 871 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92791 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 826.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:12 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 4 X 4 >UNREMARKABLE MM PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT LUMBAR REGION: SINGLE -B-KERATOACANTHOMA,-NEOPLASM PRESENT MASS, 30 X 25 X 15 MM, RED, TAN, AND FIRM; CUT SURFACE EXUDES A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22); OVERLYING SKIN HAS A SINGLE DARK BROWN AND MOIST ULCERATED AREA, 15 MM IN DIAMETER; MASS AND OVERLYING SKIN COLLECTED INTACT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 872 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92791 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 826.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:12 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 873 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : 101; 50 X 60 MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- >MASS(ES); VENTRAL-ABDOMINAL; WEEK 104; MINIMAL VERIFIED AT NECROPSY: NOT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; SORE/SCAB; AXILLARY BASOPHILIC,-MINIMAL REGION-LEFT; VERIFIED AT NECROPSY: -DEGENERATION, CYSTIC,-MINIMAL OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -ROUGH SURFACE, DIFFUSE; BILATERAL MODERATE -DILATATION, PELVIC,-MODERATE -HYALINE DROPLETS, TUBULAR EPITHELIUM,- SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL BONE, STERNUM (SB) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),- NEOPLASM PRESENT 2629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 874 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -MASS(ES); INVOLVING ENTIRE LEFT >UNREMARKABLE FORELIMB EXTENDING TO AXILLARY REGION: >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS SINGLE MASS, 60 X 40 X 30 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A TAN, DRY CRUSTED AREA, 20 X 10 MM; COLLECTED INTACT WITH MASS TRACHEA (TR) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ESOPHAGUS (ES) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); INVOLVING -B-ADENOMA,-NEOPLASM PRESENT ENTIRE LEFT ASPECT: SINGLE RED AREA, 3 MM IN DIAMETER SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 875 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL URETHRA: GREEN AND FIRM HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 22) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 876 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), THYMUS (TH), NERVE, SCIATIC (SN), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 877 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92793 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 311.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:17 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 12.6977 4.0801 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 878 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92793 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 311.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:17 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 879 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-MODERATE >SKIN & PELAGE; BROWN HAIR COAT; -VACUOLATION, HEPATOCELLULAR, MIDZONAL VENTRAL-ABDOMINAL; VERIFIED AT / CENTRILOBULAR,-SLIGHT NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, TUBULAR,-MODERATE NECROPSY: NOT OBSERVED -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- SLIGHT -NECROSIS, PAPILLARY,-MODERATELY SEVERE SPLEEN (SP) : -DEPLETION, LYMPHOCYTIC,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 880 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL SALIV GL, MANDIB (SG) : -ATROPHY,-MODERATE STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,- MODERATE ILEUM (IL) : >AUTOLYTIC URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY, RED MATERIAL 2636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 881 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO VENTRAL ABDOMINAL REGION: MOIST, BROWN MATERIAL -------------------------------------------- 2637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 882 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 883 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92795 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:00 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0216 .0032 % .0090 WEIGHT TAKEN RT ADRENAL (AD1) .0266 .0039 % .0111 WEIGHT TAKEN BRAIN (BR) 2.4060 .3558 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.1778 .3220 % .9052 WEIGHT TAKEN RT KIDNEY (KD1) 2.2393 .3311 % .9307 WEIGHT TAKEN LIVER (LI) 29.6021 4.3771 % 12.3034 WEIGHT TAKEN LUNG (LU) 1.9817 .2930 % .8236 WEIGHT TAKEN SPLEEN (SP) .7006 .1036 % .2912 WEIGHT TAKEN LF TESTIS (TE0) 2.0765 .3070 % .8631 WEIGHT TAKEN RT TESTIS (TE1) 2.0178 .2984 % .8387 WEIGHT TAKEN LF THYROID/PARA (TT0) .0205 .0030 % .0085 WEIGHT TAKEN RT THYROID/PARA (TT1) .0277 .0041 % .0115 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL 2639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 884 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92795 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:00 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-SLIGHT THYMUS (TH) : -M-THYMIC CARCINOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -HEMORRHAGE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 2640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 885 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92795 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:00 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 886 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92796 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 543.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:00 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0276 .0051 % .0113 WEIGHT TAKEN RT ADRENAL (AD1) .0283 .0052 % .0116 WEIGHT TAKEN BRAIN (BR) 2.4368 .4485 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.1147 .3892 % .8678 WEIGHT TAKEN RT KIDNEY (KD1) 2.1334 .3927 % .8755 WEIGHT TAKEN LIVER (LI) 20.3375 3.7433 % 8.3460 WEIGHT TAKEN LUNG (LU) 1.9590 .3606 % .8039 WEIGHT TAKEN SPLEEN (SP) .8078 .1487 % .3315 WEIGHT TAKEN LF TESTIS (TE0) 1.5539 .2860 % .6377 WEIGHT TAKEN RT TESTIS (TE1) 1.6589 .3053 % .6808 WEIGHT TAKEN LF THYROID/PARA (TT0) .0163 .0030 % .0067 WEIGHT TAKEN RT THYROID/PARA (TT1) .0156 .0029 % .0064 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 2642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 887 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92796 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 543.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:00 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : THYMUS (TH) : -LARGE; 16 X 12 X 4 MM >SECTION EXAMINED; TISSUE NOT PRESENT -GELATINOUS; DIFFUSE: DARK RED PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 888 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92796 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 543.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:00 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 889 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : WEEK 81; 120 X 110MM; VERIFIED AT -MINERALIZATION,-MINIMAL NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; LIMITED USE; LIMB-HIND- -DIFFUSELY DARK; ALL LOBES: BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL RIGHT; VERIFIED AT NECROPSY: NOT -CYST(S); ALL LOBES: MULTIPLE CLEAR -HYPERTROPHY, HEPATOCELLULAR, APPLICABLE FLUID FILLED CYSTS, PINPOINT AND UP TO CENTRILOBULAR POLYKARYOCYTOSIS 1 MM IN DIAMETER; COLLECTED ON ROUTINE OCCASIONALLY NOTED,-SLIGHT SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT 2645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 890 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); LEFT, -B-INTERSTITIAL CELL TUMOR,-NEOPLASM BENEATH TUNICA: MULTIPLE RED AND PRESENT YELLOW AREAS, UP TO 6 X 5 MM MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 10 X 7 X 6 MM (RIGHT IS -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM 8 X 4 X 3 MM) PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT 2646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 891 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 20 X 15 X 8 MM, TAN, LOBULATED, AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT INGUINAL REGION, EXTENDING TO RIGHT LATERAL ABDOMINAL REGION: SINGLE MASS, 135 X 115 X 55 MM (410.3 GRAMS), RED, DARK RED, TAN, GREY, LOBULATED, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 892 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 893 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92798 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 301.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:00 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 13.1989 4.3792 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF TESTIS (TE0) -------- -------- -------- NOT TAKEN RT TESTIS (TE1) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 894 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92798 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 301.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:00 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 895 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92799 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0288 .0056 % .0118 WEIGHT TAKEN RT ADRENAL (AD1) .0286 .0056 % .0117 WEIGHT TAKEN BRAIN (BR) 2.4425 .4765 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.0511 .4001 % .8398 WEIGHT TAKEN RT KIDNEY (KD1) 2.0596 .4018 % .8432 WEIGHT TAKEN LIVER (LI) 23.1666 4.5194 % 9.4848 WEIGHT TAKEN LUNG (LU) 1.9190 .3744 % .7857 WEIGHT TAKEN SPLEEN (SP) .8479 .1654 % .3471 WEIGHT TAKEN LF TESTIS (TE0) 1.7019 .3320 % .6968 WEIGHT TAKEN RT TESTIS (TE1) 1.7440 .3402 % .7140 WEIGHT TAKEN LF THYROID/PARA (TT0) .0094 .0018 % .0038 WEIGHT TAKEN RT THYROID/PARA (TT1) .0104 .0020 % .0043 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 896 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92799 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 897 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92800 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION,-MINIMAL LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE TAN AREAS, UP TO 3 MM IN -HYPERTROPHY, HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE CENTRILOBULAR POLYKARYOCYTOSIS SECTIONS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); INVOLVING ENTIRE ORGAN: -M-CARCINOMA, ISLET CELL,-NEOPLASM SINGLE MASS, 27 X 18 X 14 MM, RED, PRESENT DARK RED, TAN, GREY, LOBULATED AND -INFLAMMATION, VASCULAR,-MODERATE FIRM; SOME SECTIONS EXUDE A DARK RED FLUID; (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED 2653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 898 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92800 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATELY SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL MULTIPLE BLACK AREAS, UP TO 2 X 1 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 899 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92800 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 900 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 53; 15 X 15MM; VERIFIED AT -LARGE; ALL LOBES: THICKENED -GRANULAR CYTOPLASM, EOSINOPHILIC, NECROPSY: OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN CENTRILOBULAR,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -PIGMENT, HEPATOCELLULAR, 56; 70 X 80MM; VERIFIED AT NECROPSY: CENTRILOBULAR,-MINIMAL OBSERVED -NECROSIS, COAGULATIVE,-MODERATE -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT -LEUKOCYTOSIS, SINUSOIDAL,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -NEPHROPATHY, CHRONIC PROGRESSIVE,- -LARGE; LEFT: 30 X 20 X 15 MM (FOR SLIGHT COMPARISON, RIGHT IS 25 X 15 X 10 MM) -DILATATION, PELVIC,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT TESTIS (TE) : TESTIS (TE) : -SMALL; LEFT: 20 X 10 X 8 MM (FOR -ATROPHY/DEGENERATION,-MINIMAL COMPARISON, RIGHT IS 25 X 15 X 10 MM) MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 901 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; RIGHT: CLEAR -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, ABDOMINAL CAVITY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 22) -HEMORRHAGE,-PRESENT -DIFFUSELY RED; DARK RED CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); WITHIN ABDOMINAL CAVITY, -M-FIBROSARCOMA,-NEOPLASM PRESENT JUST CAUDAL TO LEFT KIDNEY: SINGLE MASS, 100 X 80 X 60 MM (135.7 GRAMS), RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL HIND LEFT) NOTED IN CLINICAL OBSERVATIONS PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 902 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PREPUTIAL GLAND (PG) : -LARGE; RIGHT: 40 X 30 X 15 MM (FOR COMPARISON, LEFT IS 14 X 3 X 2 MM); RIGHT IS FILLED WITH GREEN, CREAMY SEMIFLUID (CAP 21); POSSIBLY CONSISTENT WITH MASS (VENTRAL HIND RIGHT) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -FLUID-ABDOMINAL CAVITY; DARK RED SEMIFLUID -PALE MUCOUS MEMBRANES -------------------------------------------- 2658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 903 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 904 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92802 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 949.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT 2660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 905 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92802 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 949.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 2661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 906 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92802 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 949.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 907 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92803 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES, APPEAR ROUNDED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -HYPERTROPHY, HEPATOCELLULAR, MULTIPLE TAN AREAS, UP TO 3 MM IN CENTRILOBULAR POLYKARYOCYTOSIS DIAMETER, COLLECTED ON ROUTINE OCCASIONALLY NOTED,-MODERATELY SEVERE SECTIONS -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL 2663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 908 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92803 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE DARK BROWN AREA, 2 X 1 MM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 909 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92803 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 910 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92804 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 2666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 911 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92804 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 7 X 4 >UNREMARKABLE MM PITUITARY (PI) : -HYPERPLASIA,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 912 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92804 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 913 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92805 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:24 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- >EYES; RED DISCHARGE; EYE-LEFT; MINIMAL VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL 2669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 914 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92805 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:24 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 8 X 3 -ATROPHY,-MODERATELY SEVERE MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HIND FOOTPAD: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE DARK BROWN, TAN, DRY CRUSTED MODERATELY SEVERE AREA, 8 X 6 MM; COLLECTED -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,- MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, DRY MATERIAL -------------------------------------------- 2670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 915 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92805 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:24 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 916 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: NOT OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >APPEARANCE; MISSING; TEETH; VERIFIED CONSISTS PRIMARILY OF FOCAL AT NECROPSY: OBSERVED MINERALIZATION,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- BRAIN (BR) : BRAIN (BR) : ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -MOTTLED; ALL LOBES: RED AND BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >RESPIRATION; LABORED; VERIFIED AT PERIPORTAL,-MODERATE NECROPSY: NOT APPLICABLE KIDNEY (KD) : >RESPIRATION; IRREGULAR; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; THIN; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MINIMAL 2672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 917 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE >UNREMARKABLE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ULCERATED AREAS, UP 1 MM IN -EDEMA,-PRESENT DIAMETER -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT VENTRAL -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT ABDOMINAL REGION: SINGLE DARK RED DRY -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE AREA, 5 X 3 MM (CAP 22) -BACTERIAL COLONIES,-PRESENT -SUPERFICIAL CRUSTING,-MODERATELY SEVERE 2673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 918 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING RIGHT UPPER INCISOR: BROKEN AT BASE -GI TRACT-LARGE LUMEN; CECUM: UP 20 MM IN DIAMETER -------------------------------------------- 2674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 919 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 920 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92807 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 8:28 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 921 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92807 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 8:28 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 2677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 922 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92807 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 8:28 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 923 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 98; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); INGUINAL REGION-LEFT; WEEK -HYPERTROPHY, HEPATOCELLULAR, 98; 10 X 10 MM; VERIFIED AT NECROPSY: CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED OCCASIONALLY NOTED,-SLIGHT >MASS(ES); AXILLARY REGION-LEFT; WEEK -GRANULAR CYTOPLASM, EOSINOPHILIC, 103; 10 X 10 MM; VERIFIED AT NECROPSY: CENTRILOBULAR,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, MIDZONAL >SKIN & PELAGE; SORE/SCAB; AXILLARY / CENTRILOBULAR,-MINIMAL REGION-LEFT; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATELY SEVERE -PIGMENT,-MINIMAL 2679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 924 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED, SOFT AREA, 4 X 3 X 2 MM STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT AXILLARY REGION: -CYST, EPIDERMAL INCLUSION,-PRESENT SINGLE MASS, 12 X 8 X 8 MM, TAN AND -B-KERATOACANTHOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A TAN AND DRY ULCERATED AREA, 5 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 27) URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 925 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 23) MODERATE -HEMORRHAGE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: -GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY FLUID FILLED -B-ADENOMA,-NEOPLASM PRESENT CYSTS, UP TO 8 MM IN DIAMETER (CAP 22) >NOTE:>MASS #2 IS THE ADENOMA MASS #1 -MASS(ES); LEFT VENTRAL ABDOMINAL IS AN EPIDERMAL INCLUSION CYST REGION: SINGLE MASS, 15 X 11 X 8 MM, DIAGNOSED UNDER SKIN TAN, RED AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 14 X 10 X 8 MM, TAN AND FIRM; CUT SURFACE EXUDES A BROWN CREAMY SEMIFLUID; (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 926 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 927 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 85; 70 X 80 MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 928 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MASS(ES); WITHIN CHAIN: SINGLE MASS, -HYPERPLASIA, LYMPHOPLASMACYTIC,- 6 MM IN DIAMETER, DARK RED, TAN, AND MODERATE SOFT; SAME ON CUT SURFACE; COLLECTED >NOTE:>SEE PANCREAS FOR DIAGNOSIS OF INTACT MASS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL MULTIPLE BLACK AREAS, UP TO 2 X 1 MM -HYPERPLASIA, EPITHELIAL, GLANDULAR,- -THICKENED WALL; MUCOSA: DIFFUSE SLIGHT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -B-LIPOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT 2684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 929 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 80 X 80 X 25 MM, TAN AND >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 930 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 931 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -MOTTLED; ALL LOBES: TAN AND BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -HYPERTROPHY, HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >BEHAVIOR; HYPOACTIVE; VERIFIED AT OCCASIONALLY NOTED,-SLIGHT NECROPSY: NOT APPLICABLE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,- SLIGHT SPLEEN (SP) : SPLEEN (SP) : -SMALL; 27 X 6 X 4 MM -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE 2687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 932 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; LEFT: RED FLUID >UNREMARKABLE -DIFFUSELY RED; LEFT: DARK RED -LARGE; LEFT: 30 X 17 X 15 MM (RIGHT IS 22 X 13 X 12 MM) -MOTTLED; RIGHT: RED AND TAN MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 2688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 933 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- TAN ERODED AREAS, UP TO 2 MM IN MODERATELY SEVERE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -THICKENED WALL; DIFFUSE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: TAN, GREEN, AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 17 X 8 X 3 -SECRETION, DECREASED,-PRESENT MM EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 934 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 935 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92811 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/25/98 STUDY DAY OF DEATH: 250 STUDY WEEK OF DEATH: 36 TERMINAL BODY WEIGHT: 608.8 GRAMS DATE AND TIME OF NECROPSY: 12/25/98 11:31 PROSECTOR: STEVE VAN ADESTINE RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; LIMITED USE; LIMBS-HIND; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -LARGE; ALL LOBES: MARGINS ARE ROUNDED -VACUOLATION, HEPATOCELLULAR, MIDZONAL >EXCRETION; FEW FECES; VERIFIED AT -MOTTLED; ALL LOBES: RED AND TAN / CENTRILOBULAR,-MODERATELY SEVERE NECROPSY: NOT APPLICABLE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATELY SEVERE KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 54 X 12 X 6 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT BONE, FEMUR (FE) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),- NEOPLASM PRESENT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 936 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92811 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/25/98 STUDY DAY OF DEATH: 250 STUDY WEEK OF DEATH: 36 TERMINAL BODY WEIGHT: 608.8 GRAMS DATE AND TIME OF NECROPSY: 12/25/98 11:31 PROSECTOR: STEVE VAN ADESTINE RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT BONE, STERNUM (SB) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),- NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 10 X 5 X 4 MM (CAP 21) -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 937 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92811 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/25/98 STUDY DAY OF DEATH: 250 STUDY WEEK OF DEATH: 36 TERMINAL BODY WEIGHT: 608.8 GRAMS DATE AND TIME OF NECROPSY: 12/25/98 11:31 PROSECTOR: STEVE VAN ADESTINE RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) ------------------------------------------------------------------------------------------------------------------------------------ 2693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 938 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92812 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 490.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0252 .0051 % .0116 WEIGHT TAKEN RT ADRENAL (AD1) .0249 .0051 % .0114 WEIGHT TAKEN BRAIN (BR) 2.1817 .4445 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.0210 .4118 % .9263 WEIGHT TAKEN RT KIDNEY (KD1) 1.8963 .3864 % .8692 WEIGHT TAKEN LIVER (LI) 21.4730 4.3751 % 9.8423 WEIGHT TAKEN LUNG (LU) 1.8765 .3823 % .8601 WEIGHT TAKEN SPLEEN (SP) .7606 .1550 % .3486 WEIGHT TAKEN LF TESTIS (TE0) 1.8219 .3712 % .8351 WEIGHT TAKEN RT TESTIS (TE1) 1.7586 .3583 % .8061 WEIGHT TAKEN LF THYROID/PARA (TT0) .0121 .0025 % .0055 WEIGHT TAKEN RT THYROID/PARA (TT1) .0081 .0017 % .0037 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 939 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92812 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 490.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) ------------------------------------------------------------------------------------------------------------------------------------ 2695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 940 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 87; 10 X 10MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >SKIN & PELAGE; SORE/SCAB; DORSAL; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -VACUOLATION, HEPATOCELLULAR, RIGHT; VERIFIED AT NECROPSY: OBSERVED PERIPORTAL,-SLIGHT >APPEARANCE; THIN; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -MITOSIS, HEPATOCELLULAR, INCREASED,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -LARGE; BILATERAL: EACH IS 29 X 18 X SEVERE AT NECROPSY: NOT APPLICABLE 13 MM -DILATATION, PELVIC,-MINIMAL -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); DIFFUSE: TWO MASSES, BOTH -ZYMOGEN GRANULES, DECREASED,-SEVERE ARE 8 X 5 X 4 MM, BOTH ARE TAN, RED -M-CARCINOMA, ISLET CELL,- 2 NEOPLASMS AND SOFT; BOTH ARE SAME ON CUT SURFACE PRESENT (CAP 24); REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-SLIGHT 2696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 941 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATELY SEVERE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 10 MM IN DIAMETER, TAN, DARK RED, AND FIRM; CUT SURFACE EXUDES A TAN, CASEOUS SEMISOLID; COLLECTED; OVERLYING AREA HAS A DARK RED AND DRY CRUSTED AREA, 7 X 4 MM; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) 2697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 942 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 23) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 22) MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SINGLE DARK RED AND DRY AREA, 5 MM IN -ACANTHOSIS / HYPERKERATOSIS,-MODERATE DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 943 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 944 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92814 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 590.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 945 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92814 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 590.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -------------------------------------------- 2701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 946 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92814 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 590.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 947 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: NOT OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >APPEARANCE; MISSING; TEETH; VERIFIED CONSISTS PRIMARILY OF FOCAL AT NECROPSY: OBSERVED MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE 2703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 948 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); LEFT, INVOLVING ENTIRE -HYPERTROPHY, CORTICAL CELL,-SLIGHT GLAND: 10 X 8 X 4 MM, RED, DARK RED, -PIGMENT,-SLIGHT TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE RENAL) ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; LEFT: 3 MM IN DIAMETER (RIGHT >UNREMARKABLE IS 1 MM IN DIAMETER) PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 949 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: DARK RED AREA, 3 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 22) -DILATATION, SINUSOIDAL,-MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 2705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 950 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 951 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92816 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/09/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/09/99 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYROID (TY) : >AUTOLYTIC STOMACH, GL (ST) : >AUTOLYTIC 2707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 952 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92816 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/09/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/09/99 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, THORACIC CAVITY,-PRESENT >NOTE:>DIAGNOSIS LISTED UNDER AORTA LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 3 X 2 X 1 MM (CAP 21) -HEMORRHAGE,-PRESENT -DIFFUSELY RED; DARK RED AORTA (AO) : AORTA (AO) : -MASS(ES); INVOLVING ENTIRE THORACIC -M-LIPOSARCOMA,-NEOPLASM PRESENT AORTA: SINGLE MASS, 35 X 30 X 15 MM, RED, DARK RED, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF DARK RED FLUID -------------------------------------------- 2708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 953 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92816 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/09/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/09/99 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 954 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92817 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 958.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:22 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0360 .0038 % .0151 WEIGHT TAKEN RT ADRENAL (AD1) .0306 .0032 % .0128 WEIGHT TAKEN BRAIN (BR) 2.3859 .2489 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.7116 .2829 % 1.1365 WEIGHT TAKEN RT KIDNEY (KD1) 2.7885 .2909 % 1.1687 WEIGHT TAKEN LIVER (LI) 31.0099 3.2353 % 12.9971 WEIGHT TAKEN LUNG (LU) 2.1009 .2192 % .8805 WEIGHT TAKEN SPLEEN (SP) 1.1429 .1192 % .4790 WEIGHT TAKEN LF TESTIS (TE0) 1.9473 .2032 % .8162 WEIGHT TAKEN RT TESTIS (TE1) 1.8553 .1936 % .7776 WEIGHT TAKEN LF THYROID/PARA (TT0) .0216 .0023 % .0091 WEIGHT TAKEN RT THYROID/PARA (TT1) .0280 .0029 % .0117 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL 2710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 955 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92817 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 958.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:22 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -CYST(S); MID POSTERIOR ASPECT: SINGLE -B-ADENOMA,-NEOPLASM PRESENT CLEAR FLUID FILLED CYST, 2 MM IN -HYPERPLASIA,-MINIMAL DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 956 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92817 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 958.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:22 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 957 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92818 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 720.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 958 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92818 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 720.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 3 X 2 MM -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -FIBROSIS,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 959 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92818 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 720.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 960 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK LIVER (LI) : LIVER (LI) : 25; 60 X 50MM; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT OBSERVED -DEGENERATION, CYSTIC,-SLIGHT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -DEGENERATION / NECROSIS, LEFT; VERIFIED AT NECROPSY: OBSERVED CENTRILOBULAR,-SEVERE >SKIN & PELAGE; SORE/SCAB; DORSAL- -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CERVICAL-LEFT; VERIFIED AT NECROPSY: -N-CARCINOMA,-FROM ADRENAL, CORTEX OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE -THROMBUS, GLOMERULAR,-SEVERE -DEGENERATION TUBULAR,-MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATELY SEVERE -NECROSIS,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 22 MM IN DIAMETER (LEFT -DEGENERATION / NECROSIS, UNILATERAL,- IS 5 X 3 X 2 MM) SEVERE -M-CARCINOMA,-NEOPLASM PRESENT 2716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 961 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 12 X 8 >UNREMARKABLE MM; BOTH ARE SOFT MARROW, STERNUM (SE) : -N-CARCINOMA,-FROM ADRENAL, CORTEX THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -MINERALIZATION, VASCULAR,-SLIGHT MULTIPLE TAN AREAS, UP TO 5 X 2 MM -N-CARCINOMA,-FROM ADRENAL, CORTEX HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 962 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 7 X 2 -ATROPHY,-SEVERE MM EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, ADRENAL,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: DARK RED (CAP -HEMORRHAGE,-PRESENT 22) (LEFT NOT IDENTIFIED) MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); LEFT DORSAL THORACIC >NOT REQUIRED TO BE EXAMINED FOR ANIMAL REGION: SINGLE MASS, 70 X 40 X 40 MM, >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST ABRADED AREA, 12 MM IN DIAMETER; COLLECTED WITH MASS FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SINGLE DARK RED AND DRY CRUSTED AREA, -ULCERATION,-SEVERE 15 MM IN DIAMETER; COLLECTED -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-MODERATE -BACTERIA,-PRESENT 2718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 963 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 964 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92820 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:17 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0240 .0048 % .0105 WEIGHT TAKEN RT ADRENAL (AD1) .0229 .0046 % .0100 WEIGHT TAKEN BRAIN (BR) 2.2863 .4544 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 2.2802 .4531 % .9973 WEIGHT TAKEN RT KIDNEY (KD1) 2.2533 .4478 % .9856 WEIGHT TAKEN LIVER (LI) 17.6865 3.5148 % 7.7359 WEIGHT TAKEN LUNG (LU) 1.8264 .3630 % .7988 WEIGHT TAKEN SPLEEN (SP) .6612 .1314 % .2892 WEIGHT TAKEN LF TESTIS (TE0) 1.7905 .3558 % .7831 WEIGHT TAKEN RT TESTIS (TE1) 1.7871 .3551 % .7817 WEIGHT TAKEN LF THYROID/PARA (TT0) .0120 .0024 % .0052 WEIGHT TAKEN RT THYROID/PARA (TT1) .0131 .0026 % .0057 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT 2720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 965 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92820 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:17 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT STOMACH, GL (ST) : -EROSION,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER AND LOWER INCISORS -------------------------------------------- 2721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 966 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92820 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:17 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 967 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92821 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 782.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:11 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; PAW-HIND- MULTIPLE RED AREAS, UP TO 3 X 2 MM; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / LEFT; VERIFIED AT NECROPSY: OBSERVED COLLECTED ON ROUTINE SECTIONS EOSINOPHILIC CELL,-MINIMAL >APPEARANCE; MISSING; TEETH; VERIFIED -FOCUS, ALTERED HEPATOCELLULAR, AT NECROPSY: OBSERVED BASOPHILIC,-MINIMAL >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; -DEGENERATION, CYSTIC,-SLIGHT VERIFIED AT NECROPSY: OBSERVED -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 3 X 2 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SINGLE DRY BROWN CRUSTED AREA, 3 MM IN -FIBROSIS,-SEVERE DIAMETER; JOINT APPEARS SWOLLEN; -HYPERPLASIA, BONE,-MODERATELY SEVERE COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 968 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92821 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 782.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:11 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 969 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92822 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 485.0 GRAMS DATE AND TIME OF NECROPSY: 11/06/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; ATAXIC; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >EXCRETION; FEW FECES; VERIFIED AT OCCASIONALLY NOTED,-SLIGHT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >APPEARANCE; HEAD TILT; LEFT; VERIFIED EOSINOPHILIC CELL,-MINIMAL AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL 2725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 970 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92822 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 485.0 GRAMS DATE AND TIME OF NECROPSY: 11/06/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 11 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN, LOBULATED, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -LARGE; 35 X 21 X 14 MM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 31 X 21 X 9 -SECRETION, INCREASED,-PRESENT MM -GELATINOUS; BILATERAL: CLEAR AND SOFT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 971 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92822 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 485.0 GRAMS DATE AND TIME OF NECROPSY: 11/06/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 972 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92823 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 791.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:00 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED LIVER (LI) : AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 8 X 4 X 3 -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM MM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 6 X 4 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT 2728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 973 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92823 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 791.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:00 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE -------------------------------------------- 2729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 974 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92823 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 791.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:00 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 975 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 66; 50 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 4 MM IN -DEGENERATION, CYSTIC,-MINIMAL DIAMETER; COLLECTED ON ROUTINE -HYPERPLASIA, BILE DUCT,-SLIGHT SECTIONS -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -GRANULAR MATERIAL; BILATERAL: SEVERE MULTIPLE TAN AND HARD GRANULES, UP TO -DILATATION, PELVIC,-MODERATE 1 MM IN DIAMETER -REGENERATION, TUBULAR EPITHELIUM,- -DIFFUSELY LIGHT; BILATERAL: TAN MODERATE -SEMIFLUID MATERIAL-PELVIS; LEFT: -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- LIGHT GREEN SEVERE -ULCERATION, TRANSITIONAL EPITHELIUM, PELVIC,-SEVERE SPLEEN (SP) : SPLEEN (SP) : -DIFFUSELY LIGHT; TAN >UNREMARKABLE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL 2731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 976 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF -CARDIOMYOPATHY,-MODERATE BOTH VENTRICLES: MULTIPLE TAN AREAS, -MINERALIZATION, VASCULAR,-MODERATE UP TO 10 X 5 MM -MINERALIZATION,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -MINERALIZATION, GLANDULAR MUCOSA,- MULTIPLE BLACK AREAS, UP TO 1 MM IN MINIMAL DIAMETER CECUM (CE) : CECUM (CE) : -RED FOCUS(I)/AREA(S); MUCOSA, >AUTOLYTIC DIFFUSE: MULTIPLE RED AREAS, UP TO 5 X 4 MM COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 977 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL (CAP 24) -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -CONGESTION,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 22) -LYMPHANGIECTASIS,-PRESENT -CONGESTION,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 60 X 55 X 35 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK BROWN AND -LYMPHANGIECTASIS,-PRESENT TAN (CAP 25) -------------------------------------------- 2733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 978 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 979 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-SLIGHT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 980 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -N-SARCOMA,-NEOPLASM PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -MASS(ES); LEFT, INVOLVING ENTIRE -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT GLAND: SINGLE MASS, 15 X 13 X 13 MM, RED, DARK RED, GRAY, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, THORACIC CAVITY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 4 X 3 X 2 MM -HEMORRHAGE,-PRESENT -DIFFUSELY RED; DARK RED (CAP 22) LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 23) -CONGESTION,-PRESENT PERICARDIAL SAC (PD) : -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM PRESENT 2736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 981 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PERICARDIAL SAC (PD) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 53 X 45 X 38 MM, RED, DARK RED, TAN, GRAY, AND SOFT; ON SECTION EXUDES A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; DARK RED, GELATINOUS SEMIFLUID -------------------------------------------- 2737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 982 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 983 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92826 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:01 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 45; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >DISCHARGE; CLEAR ORAL; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >RESPIRATION; AUDIBLE; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-PRESENT MULTIPLE, DARK RED, PINPOINT FOCI -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 2739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 984 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92826 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:01 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE BROWN AREA, 4 X 2 MM RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); LEFT INGUINAL REGION: SINGLE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL GREENISH-TAN, CREAMY SEMIFLUID FILLED >NOTE:>SEE SKIN FOR DIAGNOSIS CYST, 8 MM IN DIAMETER (CAP 21); POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR AND MOIST -------------------------------------------- 2740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 985 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92826 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:01 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 986 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92827 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 745.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:35 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 92; 10 X 10MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >SKIN & PELAGE; SORE/SCAB; DORSAL; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, MULTIPLE RED AREAS, UP TO 2 X 1 MM; BASOPHILIC,-MINIMAL COLLECTED ON ROUTINE SECTIONS -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,- 2 NEOPLASMS PRESENT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT >NOT REQUIRED TO BE EXAMINED FOR ANIMAL POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL 2742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 987 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92827 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 745.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:35 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, CYSTIC, LOBULATED AND SOFT SKIN (SK) : SKIN (SK) : -MASS(ES); MID DORSAL THORACIC REGION: -B-KERATOACANTHOMA,-NEOPLASM PRESENT SINGLE MASS, 15 X 11 X 4 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24); OVERLYING SKIN HAS A SINGLE DRY, TAN CRUSTED AREA, 14 X 10 MM; COLLECTED WITH MASS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT PREPUTIAL GLAND (PG) : PREPUTIAL GLAND (PG) : -LARGE; LEFT: 9 X 4 X 4 MM (RIGHT IS 4 -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE X 3 X 3 MM); LEFT EXUDES A GREEN -ECTASIA, DUCTAL,-PRESENT CASEOUS MATERIAL; RIGHT EXUDES A TAN CASEOUS MATERIAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 988 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92827 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 745.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:35 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 989 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; TESTIS-LEFT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,- MODERATE -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE TESTIS (TE) : TESTIS (TE) : -MASS(ES); LEFT: SINGLE MASS, 120 X 35 -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT X 35 MM, RED, DARK RED, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE LUMBAR) 2745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 990 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -LEUKOCYTES, INCREASED, INTRAVASCULAR,- PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: -HYPERPLASIA,-MODERATE SINGLE DARK RED AREA, 3 X 2 MM STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN FOCI, PINPOINT AND UP TO 2 MM IN DIAMETER JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC 2746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 991 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : >AUTOLYTIC EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : >NOTE:>LEFT NOT IDENTIFIED DUE TO MASS -UNILATERALLY EXAMINED,-PRESENT ON LEFT TESTIS CAUSE OF DEATH (CD) : -HEMANGIOSARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT (CAP 22) -HEMORRHAGE,-PRESENT -------------------------------------------- 2747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 992 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 993 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; SORE/SCAB; VENTRAL- -COMPRESSION, VENTRAL,-PRESENT ABDOMINAL; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -CYST(S); RIGHT CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- SINGLE CLEAR FLUID FILLED CYST, 3 MM SLIGHT IN DIAMETER -CYST,-PRESENT PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); NEAR DUODENUM: SINGLE MASS, -M-CARCINOMA, ISLET CELL,-NEOPLASM 22 X 15 X 10 MM, RED, DARK RED, TAN PRESENT AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (ANTERIOR MESENTERIC) NORMAL (CAP 26) TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 15 X 4 >UNREMARKABLE MM; BOTH ARE SOFT 2749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 994 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM CECUM (CE) : CECUM (CE) : -THICKENED WALL; DIFFUSE >UNREMARKABLE PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 10 X 2 -SECRETION, DECREASED,-PRESENT MM -GELATINOUS; BILATERAL: CLEAR CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT 2750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 995 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -SUPERFICIAL CRUSTING,-MODERATE MULTIPLE BROWN, DRY AREAS, UP TO 2 X 1 MM; COLLECTED PREPUTIAL GLAND (PG) : PREPUTIAL GLAND (PG) : -LARGE; BILATERAL: EACH IS 12 X 6 X 4 -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE MM; BOTH CONTAIN A LIGHT GREEN SEMISOLID MATERIAL (CAP 25); OVERLYING SKIN HAS A DARK RED, CRUSTED AREA, 10 X 6 MM; COLLECTED INTACT WITH PREPUTIAL GLAND JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL JOINT: 15 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT DIAMETER (LEFT IS 9 MM IN DIAMETER); COLLECTED LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; BILATERAL: EACH IS UP TO 10 X -HYPERPLASIA, PLASMACYTIC,-SLIGHT 8 X 3 MM (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -GI TRACT-DARK FLUID CONTENT; DISTAL JEJUNUM AND ILEUM: CONTAIN A DARK GREEN SEMIFLUID -------------------------------------------- 2751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 996 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB), LN, ANT MES/PANC (AP) ------------------------------------------------------------------------------------------------------------------------------------ 2752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 997 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >MASS(ES); EAR-RIGHT; WEEK 47; 30 X CONSISTS PRIMARILY OF FOCAL 20MM; VERIFIED AT NECROPSY: OBSERVED MINERALIZATION,-MINIMAL >SKIN & PELAGE; SORE/SCAB; EAR-RIGHT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MASS(ES); ALL LOBES, DIFFUSE: -DEGENERATION, CYSTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT MULTIPLE MASSES, UP TO 12 X 12 X 10 -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: OBSERVED MM, TAN, RED, AND FIRM; SAME ON CUT -INFILTRATE, MACROPHAGE, PIGMENTED,- >RESPIRATION; IRREGULAR; VERIFIED AT SURFACE; COLLECTED INTACT; REGIONAL LN SLIGHT NECROPSY: NOT APPLICABLE (HEPATIC) NOT IDENTIFIED -N-SARCOMA,-NEOPLASM PRESENT >EXCRETION; FEW FECES; VERIFIED AT -ADHESION(S); LEFT LATERAL, LEFT NECROPSY: NOT APPLICABLE MEDIAN, AND RIGHT MEDIAN LOBES TO EACH >SKIN & PELAGE; OTHER; COLD TO TOUCH; OTHER: MULTIPLE GREY FIBROUS VERIFIED AT NECROPSY: NOT APPLICABLE ADHESIONS; COLLECTED INTACT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL 2753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 998 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BONE, FEMUR (FE) : -N-SARCOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE BONE, STERNUM (SB) : -N-SARCOMA,-NEOPLASM PRESENT THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT >SECTION EXAMINED; TISSUE NOT PRESENT POSITIVELY IDENTIFIED, POSSIBLE REMNANTS COLLECTED LUNG (LU) : LUNG (LU) : -MASS(ES); ATTACHED TO ALL LOBES, AND -N-SARCOMA,-NEOPLASM PRESENT PERICARDIUM: MULTIPLE MASSES, UP TO 25 X 15 X 10 MM, RED, TAN, SOFT, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -N-SARCOMA,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT 2754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 999 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -CYST(S); LEFT POSTERIOR ASPECT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT -I-SARCOMA,-NEOPLASM PRESENT PINNA (PN) : PINNA (PN) : -MASS(ES); INVOLVING ENTIRE RIGHT -M-SARCOMA,-NEOPLASM PRESENT PINNA: SINGLE MASS, 30 X 30 X 17 MM, BLACK, DRY, AND FIRM; CUT SURFACE BLACK, RED, TAN, AND FIRM; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -ANIMAL THIN -------------------------------------------- 2755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1000 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1001 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; LIMITED USE; LIMBS-ALL; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED OCCASIONALLY NOTED,-SLIGHT AT NECROPSY: NOT APPLICABLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- >EYES; RED DISCHARGE; EYE-RIGHT; MINIMAL VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; HYPOACTIVE; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL -ANGIECTASIS,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-MINIMAL 2757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1002 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, >UNREMARKABLE PROXIMAL TO PYLORIC SPHINCTER: MULTIPLE DARK RED PINPOINT FOCI DUODENUM (DU) : DUODENUM (DU) : -RED FOCUS(I)/AREA(S); SEROSAL -INFLAMMATION, CHRONIC-ACTIVE,- SURFACE: MULTIPLE DARK RED AREAS, UP MODERATELY SEVERE TO 2 MM IN DIAMETER; COLLECTED ON -ULCERATION,-MODERATE ROUTINE SECTION COLON (CO) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1003 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY MATERIAL -------------------------------------------- 2759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1004 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1005 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92832 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/12/99 10:12 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT THYROID (TY) : >AUTOLYTIC PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 9 MM, -B-ADENOMA,-NEOPLASM PRESENT SOFT, RED, DARK RED, AND TAN HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1006 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92832 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/12/99 10:12 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ILEUM (IL) : >AUTOLYTIC CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 21) -------------------------------------------- 2762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1007 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92832 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/12/99 10:12 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1008 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92833 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 823.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:26 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; LIMB-HIND-RIGHT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; -NECROSIS, INDIVIDUAL HEPATOCYTE,- VERIFIED AT NECROPSY: OBSERVED MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1009 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92833 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 823.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:26 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 13 X 8 X 8 MM (RIGHT IS -LYMPHANGIECTASIS,-PRESENT 7 X 3 X 1 MM); LEFT APPEARS CYSTIC (CAP 23) JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL JOINT: 14 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE DIAMETER (LEFT IS 10 MM IN DIAMETER); COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1010 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92833 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 823.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:26 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 2766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1011 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92834 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 662.6 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:10 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); SHOULDER-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 95; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-MODERATELY SEVERE SECTIONS -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ANTERIOR -HYPERPLASIA,-MODERATE ASPECT: SINGLE BLACK FOCUS, 1 MM IN DIAMETER 2767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1012 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92834 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 662.6 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:10 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT SHOULDER: -B-KERATOACANTHOMA,-NEOPLASM PRESENT SINGLE DRY, TAN, RAISED, AND CRUSTED AREA, 17 X 10 X 6 MM (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1013 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92834 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 662.6 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:10 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1014 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92835 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/23/99 STUDY DAY OF DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 588.6 GRAMS DATE AND TIME OF NECROPSY: 09/23/99 8:05 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : >RESPIRATION; LABORED; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; RECUMBENT; DORSAL; VERIFIED HEART (HT) : AT NECROPSY: NOT APPLICABLE -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 MM IN -B-ADENOMA,-NEOPLASM PRESENT DIAMETER, RED, DARK RED, TAN, AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1015 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92835 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/23/99 STUDY DAY OF DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 588.6 GRAMS DATE AND TIME OF NECROPSY: 09/23/99 8:05 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1016 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92836 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/10/00 11:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; PALE; ENTIRE BODY; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -DIFFUSELY LIGHT; ALL LOBES: TAN -FOCUS, ALTERED HEPATOCELLULAR, >RESPIRATION; LABORED; VERIFIED AT BASOPHILIC,-MINIMAL NECROPSY: NOT APPLICABLE -NECROSIS, COAGULATIVE,-MODERATE >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : -MASS(ES); INVOLVING ENTIRE RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- KIDNEY AND RIGHT ADRENAL: SINGLE MASS, SLIGHT 65 X 50 X 50 MM, RED, DARK RED, AND -M-LIPOSARCOMA,-NEOPLASM PRESENT SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NOT IDENTIFIED SPLEEN (SP) : SPLEEN (SP) : -LARGE; 50 X 17 X 15 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MISSING/NOT IDENTIFIED; RIGHT: NOT -UNILATERALLY EXAMINED,-PRESENT IDENTIFIED DUE TO MASS -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT 2772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1017 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92836 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/10/00 11:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, KIDNEY,-PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND DRY -ANIMAL OBESE -------------------------------------------- 2773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1018 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92836 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/10/00 11:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1019 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92837 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 461.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: KEVIN BILLINGS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT PERIPORTAL,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- PELVIS: MULTIPLE TAN PINPOINT GRANULES SLIGHT -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1020 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92837 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 461.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: KEVIN BILLINGS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 10 X 8 -B-ADENOMA,-NEOPLASM PRESENT MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- TAN, ERODED AREAS, UP TO 3 MM IN MODERATELY SEVERE DIAMETER -ULCERATION,-MODERATE -RED FOCUS(I)/AREA(S); SEROSAL -INFLAMMATION, CHRONIC-ACTIVE,- SURFACE: SINGLE DARK RED AREA, 4 MM IN MODERATELY SEVERE DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1021 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92837 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 461.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: KEVIN BILLINGS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1022 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92838 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 642.1 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 10:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 64; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: TAN AND BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-SLIGHT >SKIN & PELAGE; BROWN HAIR COAT; -HYPERPLASIA, BILE DUCT,-MINIMAL SCROTUM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; NONFORMED FECES; VERIFIED MINIMAL AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -DIFFUSELY RED; DARK RED -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE DARK BROWN AREA, 7 X 5 MM -ULCERATION,-SLIGHT 2778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1023 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92838 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 642.1 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 10:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 2 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMA- TION,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERIANAL: BROWN AND DRY -ANIMAL OBESE -------------------------------------------- 2779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1024 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92838 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 642.1 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 10:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1025 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92839 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 623 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 8:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; TAN SEVERE -DILATATION, PELVIC,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE -THROMBUS,-PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1026 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92839 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 623 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 8:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFLAMMATION, VASCULAR,-MODERATE STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE DUODENUM (DU) : -INFLAMMATION, VASCULAR,-SEVERE JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; PANCREATIC: UP TO 12 X 5 X 5 >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MM (CAP 22) >NOTE:>SEE PANCREAS -DIFFUSELY RED; PANCREATIC: DARK RED (CAP 22) GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -------------------------------------------- 2782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1027 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92839 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 623 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 8:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1028 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; LIVER (LI) : LIVER (LI) : VENTRAL-ABDOMINAL; VERIFIED AT -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT MULTIPLE DARK RED AREAS, UP TO 3 MM IN PERIPORTAL,-SEVERE NECROPSY: NOT OBSERVED DIAMETER; COLLECTED ON ROUTINE -CYST, BILIARY,-PRESENT SECTIONS -CYST(S); VISCERAL SURFACE OF LEFT LATERAL LOBE, NEAR HILUS: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 2784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1029 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 -ULCERATION,-MINIMAL MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- RED AND TAN ERODED AREAS, UP TO 5 X 2 MODERATELY SEVERE MM -ULCERATION,-MODERATE -THICKENED WALL; DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1030 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST MATERIAL -------------------------------------------- 2786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1031 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1032 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92841 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:16 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, RED, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1033 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92841 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:16 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1034 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92842 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 689.8 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 7:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 77; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; RED HAIR COAT; HEAD- -FOCUS, ALTERED HEPATOCELLULAR, ENTIRE; VERIFIED AT NECROPSY: OBSERVED BASOPHILIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >APPEARANCE; CONVULSIONS; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: LEFT IS 20 X 17 X 5 -ATROPHY/DEGENERATION,-MODERATELY MM, RIGHT IS 20 X 14 X 4 MM; BOTH ARE SEVERE SOFT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1035 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92842 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 689.8 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 7:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC -B-KERATOACANTHOMA,-NEOPLASM PRESENT REGION: SINGLE DARK RED, DRY, AND RAISED AREA, 10 X 8 X 6 MM (CAP 22) URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,- PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, -INFLAMMATION / NECROSIS, FAT,-MODERATE CRANIAL TO RIGHT TESTIS: SINGLE MASS, 25 X 25 X 20 MM, YELLOW, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1036 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92842 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 689.8 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 7:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-NASAL; CLEAR AND MOIST -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED AND MOIST -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 2792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1037 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92843 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 13:44 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFLAMMATION, GRANULOMATOUS,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 3 MM IN DIAMETER -ANGIECTASIS,-PRESENT ILEUM (IL) : >AUTOLYTIC 2793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1038 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92843 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 13:44 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 2794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1039 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92843 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 13:44 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1040 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92844 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/19/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 473.5 GRAMS DATE AND TIME OF NECROPSY: 11/19/99 9:50 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT OF RIGHT MEDIAN LOBE: SINGLE TAN, -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: OBSERVED SLIGHTLY RAISED AREA, 6 X 4 X 4 MM; EOSINOPHILIC CELL,-SLIGHT >EYES; RED DISCHARGE; EYE-RIGHT; COLLECTED ON ROUTINE SECTION -DEGENERATION, CYSTIC,-MODERATELY VERIFIED AT NECROPSY: OBSERVED SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT 2796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1041 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92844 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/19/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 473.5 GRAMS DATE AND TIME OF NECROPSY: 11/19/99 9:50 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 9 X 8 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, GRAY, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X 5 >UNREMARKABLE MM EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY CRUSTED MATERIAL -ANIMAL THIN -------------------------------------------- 2797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1042 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92844 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/19/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 473.5 GRAMS DATE AND TIME OF NECROPSY: 11/19/99 9:50 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1043 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92845 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/12/00 STUDY DAY OF DEATH: 664 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 653.2 GRAMS DATE AND TIME OF NECROPSY: 02/12/00 8:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -VACUOLATION, HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE PERIPORTAL,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE ADRENAL, CORTEX (AC) : -MINERALIZATION,-MINIMAL -FIBROSIS,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1044 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92845 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/12/00 STUDY DAY OF DEATH: 664 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 653.2 GRAMS DATE AND TIME OF NECROPSY: 02/12/00 8:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, DARK RED, TAN -ANGIECTASIS,-PRESENT AND SOFT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,- SLIGHT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -------------------------------------------- 2800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1045 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92845 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/12/00 STUDY DAY OF DEATH: 664 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 653.2 GRAMS DATE AND TIME OF NECROPSY: 02/12/00 8:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1046 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >WART LIKE LESION(S); DORSAL; WEEK 80; -FOCUS, ALTERED HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -CYST(S); BILATERAL CORTICAL SURFACES, -NEPHROPATHY, CHRONIC PROGRESSIVE,- EXTENDING TO MEDULLA: MULTIPLE CLEAR SEVERE FLUID FILLED CYSTS, UP TO 3 MM IN -DILATATION, PELVIC,-SLIGHT DIAMETER -CYST,-PRESENT -LARGE PELVIS(ES); BILATERAL -B-PAPILLOMA, TRANSITIONAL CELL,- -ROUGH SURFACE, DIFFUSE; BILATERAL NEOPLASM PRESENT -LARGE; BILATERAL: EACH IS 32 X 22 X 14 MM -DIFFUSELY LIGHT; BILATERAL: TAN SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 6 MM IN -INFLAMMATION, VASCULAR,-SLIGHT DIAMETER (CAP 24) -THROMBUS,-PRESENT ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL 2802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1047 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT -MYELOFIBROSIS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : HEART (HT) : -LARGE; 30 X 20 X 14 MM -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT 2803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1048 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 3 X 2 MM PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 MM IN -HYPERPLASIA,-MODERATELY SEVERE DIAMETER PITUITARY (PI) : PITUITARY (PI) : -LARGE; 4 X 4 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE -MINERALIZATION, GLANDULAR MUCOSA,- SLIGHT ILEUM (IL) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT SHOULDER REGION: SINGLE TAN, DRY AND CAULIFLOWER- LIKE CRUSTED AREA, 25 X 20 MM (CAP 30) PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN -LARGE; 33 X 23 X 9 MM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 43 X 33 X >UNREMARKABLE 22 MM EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MODERATE 2804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1049 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 22) -HEMORRHAGE,-PRESENT -DIFFUSELY RED -LYMPHANGIECTASIS,-MODERATELY SEVERE LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; RIGHT: 4 X 3 X 3 MM (LEFT, SEE -HYPERPLASIA, LYMPHOPLASMACYTIC,- CYST COMMENT) (CAP 26) MODERATE -CYST(S); INVOLVING ENTIRE LEFT: -HEMORRHAGE,-PRESENT SINGLE CLEAR FLUID FILLED CYST, 6 X 3 -LYMPHANGIECTASIS,-MODERATE X 3 MM (CAP 26) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYST(S); ATTACHED TO CRANIAL END OF -LYMPHANGIECTASIS,-PRESENT LEFT: SINGLE CLEAR FLUID FILLED CYST, -HYPERPLASIA, LYMPHOPLASMACYTIC,- 3 X 3 X 2 MM (RIGHT IS NORMAL) (CAP MODERATELY SEVERE 29) CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -LARGE VESSELS; MESENTERY, DIFFUSE: UP -INFLAMMATION, VASCULAR,-MODERATE TO 6 MM IN DIAMETER (CAP 25) -THROMBUS,-PRESENT AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 5 MM IN >UNREMARKABLE DIAMETER (CAP 23) -FIRM; DIFFUSE URETER (UE) : URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 2 MM -UNILATERALLY EXAMINED,-PRESENT IN DIAMETER (CAP 27) VAS DEFERENS (VS) : VAS DEFERENS (VS) : -LARGE; BILATERAL: EACH IS UP TO 3 MM >UNREMARKABLE IN DIAMETER (CAP 28) 2805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1050 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -FLUID-ABDOMINAL CAVITY; DARK RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1051 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92847 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 579.0 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; RED DISCHARGE; EYE-LEFT; -NECROSIS, INDIVIDUAL HEPATOCYTE,- VERIFIED AT NECROPSY: OBSERVED MINIMAL >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT OBSERVED EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; LEFT -NEPHROPATHY, CHRONIC PROGRESSIVE,- -SMALL; LEFT: 20 X 10 X 10 MM (RIGHT MODERATE IS 25 X 17 X 15 MM) -DILATATION, PELVIC,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 2807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1052 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92847 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 579.0 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF LEFT VENTRICLE: MULTIPLE GREY AREAS, UP TO 5 X 2 MM THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 9 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT LOBULATED, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT, HIND FOOT: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE RED AND DARK RED CRUSTED AREA, MODERATELY SEVERE 11 MM IN DIAMETER, COLLECTED -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1053 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92847 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 579.0 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1054 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92848 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; LIMITED USE; PAW-HIND- KIDNEY (KD) : KIDNEY (KD) : LEFT; VERIFIED AT NECROPSY: NOT -LARGE PELVIS(ES); RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- APPLICABLE MINIMAL >APPEARANCE; THIN; VERIFIED AT -DILATATION, PELVIC,-MODERATE NECROPSY: OBSERVED PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED FOCUS, 2 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 11 X 9 X 6 MM (RIGHT IS -LYMPHANGIECTASIS,-PRESENT 4 X 3 X 2 MM) (CAP 23) JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL: 16 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT DIAMETER; COLLECTED -FIBROSIS,-MODERATELY SEVERE -HYPERPLASIA, BONE,-SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN 2810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1055 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92848 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1056 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92849 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 476.9 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -NECROSIS, COAGULATIVE,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT >BEHAVIOR; HYPOACTIVE; MODERATE; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -GRANULAR MATERIAL; RIGHT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- PELVIS: MULTIPLE TAN PINPOINT GRANULES MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 8 MM, -B-ADENOMA,-NEOPLASM PRESENT TAN, RED, DARK RED, AND SOFT 2812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1057 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92849 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 476.9 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EDEMA,-MODERATE DARK BROWN AREA, 2 X 1 MM -INFLAMMATION, ACUTE,-SLIGHT -EROSION,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1058 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92849 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 476.9 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1059 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : SPLEEN (SP) : -MASS(ES); INVOLVING ENTIRE SPLEEN: -HEMATOPOIESIS, EXTRAMEDULLARY, SINGLE MASS, 55 X 33 X 33 MM, RED, INCREASED,-MODERATE DARK RED, TAN AND FIRM; SAME ON CUT -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE PANCREATIC) PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT -INFLAMMATION, VASCULAR,-MODERATE 2815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1060 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL -CYST,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL MULTIPLE DARK BROWN AREAS, UP TO 1 MM IN DIAMETER PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SPLEEN,-PRESENT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; PANCREATIC: UP TO 7 X 5 X 5 MM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL (CAP 22) >NOTE:>SEE PANCREAS FOR DIAGNOSIS OF -DIFFUSELY RED; PANCREATIC: DARK RED MASS. LYMPH NODE NOT INVOLVED. 2816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1061 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; MOIST, YELLOW MATERIAL -------------------------------------------- 2817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1062 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1063 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92851 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 788.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:53 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); VENTRAL- LIVER (LI) : LIVER (LI) : ABDOMINAL; WEEK 102; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 X 2 -VACUOLATION, HEPATOCELLULAR, MM; COLLECTED ON ROUTINE SECTIONS PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : SPLEEN (SP) : -ADHESION(S); PARIETAL SURFACE -HEMATOPOIESIS, EXTRAMEDULLARY, ATTACHED TO SURROUNDING ADIPOSE INCREASED,-SLIGHT TISSUE: MULTIPLE GRAY FIBROUS -FIBROSIS, CAPSULAR,-PRESENT ADHESIONS; COLLECTED INTACT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 3 MM IN MINIMAL DIAMETER; SOME ARE FIRM -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1064 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92851 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 788.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:53 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : THYROID (TY) : -MASS(ES); RIGHT: SINGLE MASS, 6 X 4 X -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM 3 MM, RED, TAN AND FIRM; SAME ON CUT PRESENT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 23) SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); MID ABDOMINAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT REGION: SINGLE BROWN, TAN, DRY CRUSTED -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM AREA, 6 MM IN DIAMETER (CAP 24) PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE -HEMORRHAGE,-PRESENT -LYMPHANGIECTASIS,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MULTIPLE DARK BROWN AREAS, UP TO 3 MM -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1065 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92851 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 788.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:53 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1066 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92852 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/07/99 STUDY DAY OF DEATH: 475 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 544.2 GRAMS DATE AND TIME OF NECROPSY: 08/07/99 8:05 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT SURFACE OF LEFT LATERAL LOBE: MULTIPLE -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE DARK RED AREAS, UP TO 2 X 1 MM; PERIPORTAL,-SLIGHT >APPEARANCE; HUNCHED; VERIFIED AT COLLECTED ON ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT -DEGENERATION, CYSTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 2822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1067 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92852 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/07/99 STUDY DAY OF DEATH: 475 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 544.2 GRAMS DATE AND TIME OF NECROPSY: 08/07/99 8:05 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE DARK BROWN AND BLACK ERODED AREAS, UP -ULCERATION,-MODERATE TO 6 X 4 MM -INFLAMMATION, CHRONIC-ACTIVE,- -THICKENED WALL; DIFFUSE MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1068 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92852 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/07/99 STUDY DAY OF DEATH: 475 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 544.2 GRAMS DATE AND TIME OF NECROPSY: 08/07/99 8:05 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1069 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92853 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 691.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, CHRONIC-ACTIVE,- DARK RED ERODED AREA, 5 MM IN DIAMETER MODERATELY SEVERE -ULCERATION,-MODERATE 2825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1070 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92853 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 691.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1071 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL -NECROSIS, COAGULATIVE,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1072 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL -CYST,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO >UNREMARKABLE DARK BROWN AREAS, EACH IS 2 X 1 MM JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1073 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ EPIDIDYMIDES (EP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -------------------------------------------- 2829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1074 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV) ------------------------------------------------------------------------------------------------------------------------------------ 2830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1075 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: NOT OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >SKIN & PELAGE; ROUGH HAIR COAT; CONSISTS PRIMARILY OF FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE MINERALIZATION,-MINIMAL >WART LIKE LESION(S); VENTRAL-ABDOMINAL- BRAIN (BR) : BRAIN (BR) : LEFT; WEEK 69; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED KIDNEY (KD) : >APPEARANCE; HUNCHED; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL TESTIS (TE) : TESTIS (TE) : -CONTAINS FLUID; BILATERAL, BENEATH -ATROPHY/DEGENERATION,-SEVERE TUNICA: CLEAR FLUID THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 2831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1076 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, SOFT AND LOBULATED HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); VENTRAL ABDOMINAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT REGION: SINGLE TAN AND RAISED AREA, 3 -B-KERATOACANTHOMA,-NEOPLASM PRESENT X 3 X 2 MM (CAP 21); COLLECTED; POSSIBLY CONSISTENT WITH WART-LIKE LESION AS NOTED IN CLINICAL OBSERVATIONS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -INFLAMMATION / NECROSIS, FAT,-MODERATE NEAR LEFT EPIDIDYMIS: SINGLE MASS, 11 X 4 X 3 MM, TAN, YELLOW AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1077 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF NEAR BASE -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -------------------------------------------- 2833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1078 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1079 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92856 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 606.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; BENT TAIL; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); INGUINAL REGION-LEFT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 99; VERIFIED AT NECROPSY: NOT OBSERVED EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -CYST(S); LEFT CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- SINGLE CLEAR FLUID FILLED CYST, 3 MM MODERATELY SEVERE IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : PANCREAS (PA) : -LARGE VESSELS; UP TO 3 MM IN DIAMETER -INFLAMMATION, VASCULAR,-SEVERE (CAP 24) LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: RED AND TAN >UNREMARKABLE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE 2835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1080 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92856 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 606.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -CYST(S); LEFT POSTERIOR ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -HYPERPLASIA, EPITHELIAL, GLANDULAR,- SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 4 X 4 X 3 MM (CAP 22) -LYMPHANGIECTASIS,-MODERATE LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 5 X 4 -INFILTRATE, MACROPHAGE, PIGMENTED,- X 3 MM (CAP 23) SLIGHT -MOTTLED; BILATERAL: RED AND TAN -LYMPHANGIECTASIS,-MODERATELY SEVERE CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -LARGE VESSELS; MESENTERY: UP TO 4 MM -INFLAMMATION, VASCULAR,-MODERATE IN DIAMETER (CAP 25) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; 20 MM AND 40 MM FROM TIP: TWO BENT AREAS; COLLECTED -BONE MARROW SMEAR TAKEN -------------------------------------------- 2836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1081 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92856 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 606.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1082 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92857 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 570.5 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 9:35 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >EYES; SQUINTED; PERI-ORBITAL; VERIFIED PERIPORTAL,-MINIMAL AT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >EYES; RED DISCHARGE; EYES; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL 2838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1083 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92857 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 570.5 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 9:35 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 8 X 7 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL URINARY BLADDER (UB) : URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE TAN >UNREMARKABLE AND HARD CALCULUS, 6 MM IN DIAMETER PROSTATE (PR) : PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X 6 -SECRETION, INCREASED,-PRESENT MM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 12 X >TISSUE MISSING 7 X 4 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL 2839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1084 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92857 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 570.5 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 9:35 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1085 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); DORSAL; WEEK 87; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 91; 15 X 20MM; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: BROWN AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT OBSERVED -ROUGH SURFACE, DIFFUSE; ALL LOBES -NECROSIS, INDIVIDUAL HEPATOCYTE,- >EXCRETION; FEW FECES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT PERIPORTAL,-MODERATELY SEVERE NECROPSY: NOT OBSERVED -HYPERPLASIA, BILE DUCT,-MODERATELY >RESPIRATION; AUDIBLE; VERIFIED AT SEVERE NECROPSY: NOT APPLICABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -HYPERPLASIA, ISLET CELLS,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 2841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1086 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFLAMMATION, GRANULOMATOUS,-SLIGHT >NOTE:>SOME INFLAMMATORY FOCI CONTAIN REFRACTILE FOREIGN MATERIAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, GRAY, AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT -B-RHABOMYOMA,-NEOPLASM PRESENT 2842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1087 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -CRUSTED AREA(S); LEFT DORSAL LUMBAR REGION: SINGLE DRY BROWN CRUSTED AREA, 5 MM IN DIAMETER; POSSIBLY CONSISTENT WITH WART-LIKE LESION NOTED IN CLINICAL OBSERVATION (CAP 26) PROSTATE (PR) : PROSTATE (PR) : -LARGE; 25 X 20 X 12 MM -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -GELATINOUS; DIFFUSE: TAN SEMINAL VESICLES (SV) : SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: TAN -EDEMA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : MAMMARY, MALE (MM) : -CYST(S); BILATERAL, AXILLARY REGION: -GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY SEMIFLUID FILLED -M-CARCINOMA,-NEOPLASM PRESENT CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) >NOTE:>MASS #1 IS THE CARCINOMA. SEE -MASS(ES); RIGHT AXILLARY REGION: SKIN FOR DIAGNOSIS OF MASS #2. SINGLE MASS, 35 X 25 X 13 MM, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS SEMISOLID (CAP 24); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 10 X 10 X 6 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1088 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1089 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92859 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 423.6 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:49 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREA, UP TO 3 MM IN -HYPERPLASIA, BILE DUCT,-MINIMAL DIAMETER; SOME AREAS APPEAR TO BE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : KIDNEY (KD) : -DEPRESSED FOCUS(I)/AREA(S); -NEPHROPATHY, CHRONIC PROGRESSIVE,- BILATERAL, CORTICAL SURFACE: MULTIPLE MINIMAL AREAS, UP TO 2 MM IN DIAMETER SPLEEN (SP) : SPLEEN (SP) : -SMALL; 30 X 7 X 4 MM -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : TESTIS (TE) : >NOTE:>TRIMMING COMMENT: ONE TESTIS -UNILATERALLY EXAMINED,-PRESENT MISSING FROM WET TISSUE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 2845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1090 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92859 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 423.6 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:49 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT LOBULATED AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : EPIDIDYMIDES (EP) : >NOTE:>TRIMMING COMMENT: ONE EPIDIDYMIS -UNILATERALLY EXAMINED,-PRESENT MISSING FROM WET TISSUE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1091 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92859 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 423.6 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:49 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1092 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); VENTRAL-THORACIC- LIVER (LI) : LIVER (LI) : LEFT; WEEK 96; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NOT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >EXCRETION; FEW FECES; VERIFIED AT EOSINOPHILIC CELL,-MODERATE NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >APPEARANCE; LIMITED USE; LIMBS-HIND; -HYPERPLASIA, BILE DUCT,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR,-MINIMAL >SKIN & PELAGE; ROUGH HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EYES; RED DISCHARGE; EYES; VERIFIED AT MINIMAL NECROPSY: OBSERVED SPLEEN (SP) : SPLEEN (SP) : >BEHAVIOR; ATAXIC; VERIFIED AT -MASS(ES); DIFFUSE: MULTIPLE MASSES, -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE UP TO 10 MM IN DIAMETER, RED, TAN, AND INCREASED,-MODERATE FIRM; SAME ON CUT SURFACE; COLLECTED -M-OSTEOSARCOMA,-NEOPLASM PRESENT INTACT; REGIONAL LN (SEE PANCREATIC) -MASS #2; INVOLVING ENTIRE CRANIAL END: SINGLE MASS, 10 MM IN DIAMETER, RED, TAN, HARD, BONE-LIKE; SAME ON CUT SURFACE, ALTHOUGH MASS WAS VERY DIFFICULT TO SECTION (CAP 25); REGIONAL LN (SEE PANCREATIC) PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 2848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1093 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); ALONG SPLEEN: SINGLE MASS, -M-CARCINOMA, ISLET CELL,-NEOPLASM 8 MM IN DIAMETER, RED, GRAY, AND SOFT; PRESENT SAME ON CUT SURFACE (CAP 23); REGIONAL -INFILTRATION, FAT, INTERSTITIAL,- LN (SEE PANCREATIC) MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING APPROXIMATELY ONE- -VACUOLATION, FOCAL,-MINIMAL HALF OF LEFT: SINGLE MASS, 5 MM IN -N-SARCOMA,-NEOPLASM PRESENT DIAMETER, TAN, HARD, AND BONE-LIKE; MASS VERY DIFFICULT TO SECTION, PORTION OF REMAINING ADRENAL EXUDED OUT OF CORTEX AREA IN AN ATTEMPT TO SECTION MASS (CAP 26); REGIONAL LN (SEE RENAL) TESTIS (TE) : TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 13 X 8 -ATROPHY/DEGENERATION,-MODERATE MM; BOTH ARE SOFT SPINAL CORD (SC) : -NECROSIS / HEMORRHAGE, WHITE MATTER,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -N-SARCOMA,-NEOPLASM PRESENT 2849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1094 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MASS(ES); ALL LOBES, INVOLVING MOST OF LUNG TISSUE: MULTIPLE MASSES, UP TO 15 X 10 X 8 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (SEE TRACHEOBRONCHIAL) -MOTTLED; ALL LOBES: RED, DARK RED, AND TAN -UNCOLLAPSED; ALL LOBES HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE RED AND TAN ERODED AREAS, UP TO 5 MM -ULCERATION,-MINIMAL IN DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -THICKENED WALL; DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, SPLEEN,-PRESENT LN, RENAL (RL) : -N-SARCOMA,-NEOPLASM PRESENT 2850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1095 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, RENAL (RL) : -LARGE; LEFT: 10 MM IN DIAMETER (RIGHT IS 5 X 3 X 2 MM) (CAP 27) LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; (CAP 24) -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LN, TRACHEOBRON (TB) : LN, TRACHEOBRON (TB) : -LARGE; UP TO 8 X 5 X 3 MM (CAP 22) -DILATATION, SINUSOIDAL,-PRESENT -DIFFUSELY RED -HEMORRHAGE,-SLIGHT -N-SARCOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -------------------------------------------- 2851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1096 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2852 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume VIII of IX Page 2853 of 4068 Covance 6329-183 3M T-6295 VOLUME VIII OF IX CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 2855 Individual Anatomic Pathology Data ..................................................................... 2856 2854 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 2855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1097 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92861 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 226.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:46 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.8642 3.4757 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1098 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92861 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 226.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:46 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1099 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92862 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 281.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0474 .0169 % .0231 WEIGHT TAKEN RT ADRENAL (AD1) .0341 .0121 % .0166 WEIGHT TAKEN BRAIN (BR) 2.0523 .7298 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1945 .4248 % .5820 WEIGHT TAKEN RT KIDNEY (KD1) 1.2160 .4324 % .5925 WEIGHT TAKEN LIVER (LI) 9.3278 3.3171 % 4.5450 WEIGHT TAKEN LUNG (LU) 1.2262 .4361 % .5975 WEIGHT TAKEN LF OVARY (OV0) .0431 .0153 % .0210 WEIGHT TAKEN RT OVARY (OV1) .0686 .0244 % .0334 WEIGHT TAKEN SPLEEN (SP) .3610 .1284 % .1759 WEIGHT TAKEN LF THYROID/PARA (TT0) .0145 .0052 % .0071 WEIGHT TAKEN RT THYROID/PARA (TT1) .0135 .0048 % .0066 WEIGHT TAKEN UTERUS (UT) .9292 .3304 % .4528 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR 2858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1100 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92862 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 281.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1101 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92863 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:44 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MISSING; TAIL-DISTAL; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; BLACK SKIN; TAIL; SURFACE OF LEFT MEDIAN LOBE: SINGLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- VERIFIED AT NECROPSY: OBSERVED DARK RED FOCUS, 3 MM IN DIAMETER (CAP MINIMAL 21) -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -IRREGULAR SHAPE -HEMATOPOIESIS, EXTRAMEDULLARY, -LIGHT FOCUS(I)/AREA(S); DIFFUSE: INCREASED,-MODERATELY SEVERE MULTIPLE TAN AREAS, UP TO 6 X 3 MM -FIBROSIS, CAPSULAR,-PRESENT -ADHESION(S); PARIETAL SURFACE TO LEFT OVARY: MULTIPLE TAN AND FIBROUS ADHESIONS; COLLECTED INTACT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1102 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92863 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:44 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); ATTACHED TO DORSAL SIDE OF -B-ADENOMA,-NEOPLASM PRESENT RIGHT ASPECT: SINGLE MASS, 5 X 3 X 2 MM, RED, DARK RED AND SOFT RECTUM (RE) : -INFLAMMATION, VASCULAR,-SLIGHT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PECTORAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 30 X 20 X 10 MM, TAN AND -B-FIBROADENOMA,-NEOPLASM PRESENT SOFT; SAME ON CUT SURFACE; COLLECTED; -HYPERPLASIA, GLANDULAR,-MODERATE REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION URINARY BLADDER (UB) : -INFLAMMATION, VASCULAR,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -VASCULITIS, SYSTEMIC,-PRESENT 2861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1103 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92863 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:44 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ TAIL (TI) : TAIL (TI) : -DIFFUSELY RED; 60 MM FROM REMAINING -INFLAMMATION, VASCULAR,-SLIGHT TIP: DARK RED; COLLECTED LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; PANCREATIC (CAP 22) -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; DISTAL ONE THIRD: MISSING; REMAINING PORTION COLLECTED -------------------------------------------- 2862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1104 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92863 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:44 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92864 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 218.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.3146 3.8124 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 2864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1106 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92864 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 218.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 2865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1107 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92865 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: DAVID SCHUETTE RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LIVER (LI) : -CONGESTION,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -CONGESTION,-PRESENT -PIGMENT,-MINIMAL SPINAL CORD (SC) : -HEMORRHAGE,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED -CONGESTION,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 2866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1108 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92865 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: DAVID SCHUETTE RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 2867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1109 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92865 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: DAVID SCHUETTE RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1110 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92866 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -DEGENERATION, RETINAL,-MODERATE BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); RIGHT ON CORTICAL SURFACE -NEPHROPATHY, CHRONIC PROGRESSIVE,- NEAR CAUDAL POLE: SINGLE CLEAR FLUID MINIMAL FILLED CYST, 1 MM IN DIAMETER -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 8 MM IN DIAMETER 2869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1111 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92866 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 5 MM, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1112 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92866 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1113 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92867 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 104; LIVER (LI) : 15 X 15MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MODERATE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE 2872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1114 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92867 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 4 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 20 X 15 X 10 MM, DARK -HYPERPLASIA, GLANDULAR,-MODERATE RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HEMORRHAGE,-PRESENT COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1115 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92867 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1116 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92868 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:24 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL 2875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1117 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92868 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:24 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN >UNREMARKABLE OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 8 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 1 MM IN -HYPERPLASIA,-MINIMAL DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ABDOMINAL REGION: MULTIPLE -CYST / GALACTOCELE,-PRESENT WHITE FLUID FILLED CYSTS, UP TO 10 X 6 -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE X 2 MM (CAP 22) -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR AND DARK RED FLUID SLIGHT FILLED CYSTS, UP TO 3 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1118 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92868 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:24 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1119 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92869 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 435.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -CYST,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 2878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1120 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92869 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 435.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1121 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92869 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 435.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1122 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92870 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 8:10 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 99; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; MALOCCLUSION; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT PERIPORTAL,-SLIGHT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; ATAXIC; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED -CYST, BILIARY,-PRESENT AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -MINERALIZATION, PELVIC,-MINIMAL AT NECROPSY: NOT APPLICABLE -MINERALIZATION, TUBULAR,-MINIMAL >MASS(ES); INGUINAL REGION-LEFT; WEEK -HYPERPLASIA, TRANSITIONAL CELL,- 105; VERIFIED AT NECROPSY: OBSERVED MINIMAL >APPEARANCE; THIN; VERIFIED AT SPLEEN (SP) : NECROPSY: OBSERVED -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -PIGMENT,-SLIGHT 2881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1123 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92870 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 8:10 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 8 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, GRAY AND FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 30 X 30 X 10 MM, TAN AND -HYPERPLASIA, GLANDULAR,-MODERATE FIRM; SAME ON CUT SURFACE; COLLECTED; -B-ADENOMA,-NEOPLASM PRESENT REGIONAL LN (AXILLARY) NORMAL (CAP 21) >NOTE:>MASSES 1 AND 2 ARE PRIMARILY -THICKENED; ALL REGIONS, DIFFUSE: TAN FIBROADENOMAS A SMALL ADENOMA AND FIRM; COLLECTED ON ROUTINE SECTION WAS ALSO NOTED IN MASS 2 -MASS #2; PERINEAL REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND FIRM, SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (INGUINAL-LEFT) AS NOTED IN CLINICAL OBSERVATIONS 2882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1124 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92870 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 8:10 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: -B-POLYP, ENDOMETRIAL, STROMAL,- SINGLE CLEAR FLUID FILLED CYST, 18 X 5 NEOPLASM PRESENT X 5 MM -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SEVERE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE CONTAINS A TAN MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY MATERIAL -ANIMAL THIN -------------------------------------------- 2883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1125 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92870 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 8:10 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1126 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92871 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:46 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 50 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR, 94; 50 X 50MM; VERIFIED AT NECROPSY: MULTIPLE TAN AREAS, UP TO 3 MM IN PERIPORTAL,-SLIGHT OBSERVED DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / SECTIONS EOSINOPHILIC CELL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 9 MM IN DIAMETER -DEGENERATION, CYSTIC / HEMORRHAGIC,- -SMALL; LEFT: 3 X 2 X 2 MM SEVERE -PIGMENT,-SLIGHT 2885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1127 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92871 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:46 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 70 X 60 X 30 MM, TAN, -CYST / GALACTOCELE,-PRESENT FIRM, AND LOBULATED; SAME ON CUT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SURFACE; COLLECTED; REGIONAL LN -HYPERPLASIA, GLANDULAR,-SLIGHT (AXILLARY) NORMAL (CAP 22) >NOTE:>MASS #2 IS THE CARCINOMA -MASS #2; LEFT PERINEAL REGION: SINGLE MASS, DARK RED, TAN, VASCULAR, FIRM, AND LOBULATED, 60 X 50 X 30 MM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 23); OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST ULCERATED AREA, 10 MM IN DIAMETER; COLLECTED WITH MASS 2886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1128 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92871 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:46 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 15 X 10 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 23) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR AND RED FLUID FILLED MODERATE CYSTS, UP TO 4 MM IN DIAMETER VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT LN, INGUINAL (IN) : -N-CARCINOMA,-NEOPLASM PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>TRIMMING COMMENT: ANIMAL ID MISSING AT TRIMMING -------------------------------------------- 2887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1129 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92871 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:46 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1130 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92872 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 12/28/99 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 41; BRAIN (BR) : BRAIN (BR) : 80 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 83; 30 X 20MM; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE OBSERVED SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 6 MM (RIGHT IS 5 -DEGENERATION, CYSTIC / HEMORRHAGIC,- MM IN DIAMETER) SEVERE OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 2889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1131 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92872 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 12/28/99 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 80 X 75 X 45 MM (138.2 -B-FIBROADENOMA,-NEOPLASM PRESENT GRAMS), RED, DARK RED, GREY, TAN, >NOTE:>MASS #1 IS THE FIBROADENOMA LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DARK RED, TAN AND DRY CRUSTED AREA, 30 X 25 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; LEFT INGUINAL REGION, CAUDAL TO MASS 1: SINGLE MASS, 25 X 25 X 20 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE (COLLECTED); REGIONAL LN (INGUINAL) NORMAL (CAP 21) UTERUS (UT) : UTERUS (UT) : -CYST(S); PROXIMAL END OF LEFT HORN, -DILATATION,-MODERATELY SEVERE WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 9 X 5 X 5 MM 2890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1132 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92872 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 12/28/99 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1133 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92872 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 12/28/99 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1134 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92873 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ALOPECIA; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALONG MARGIN OF -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL RIGHT LATERAL LOBE, NEAR HILUS: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DARK RED AREA, 10 X 5 MM (CAP 23) EOSINOPHILIC CELL,-MINIMAL -CYST(S); PAPILLARY PROCESS: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR FLUID FILLED CYST, 1 MM IN BASOPHILIC,-MINIMAL DIAMETER (CAP 22) -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -INFARCT,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL 2893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1135 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92873 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT SPINAL CORD (SC) : -DEGENERATION, AXONAL,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 5 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BLACK AREAS, UP TO 3 X 1 MM SKIN (SK) : SKIN (SK) : -ALOPECIA-DIFFUSE; ENTIRE VENTRAL >UNREMARKABLE SURFACE: COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); BILATERAL INGUINAL REGIONS: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY FLUID FILLED -B-FIBROADENOMA,-NEOPLASM PRESENT CYSTS, UP TO 10 MM IN DIAMETER (CAP 24) UTERUS (UT) : -DILATATION,-MODERATE 2894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1136 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92873 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -CYST(S); LEFT HORN WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 6 X 3 X 3 MM VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1137 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92873 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1138 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92874 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:26 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 93; BRAIN (BR) : BRAIN (BR) : 40 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SEVERE -B-LIPOMA,-NEOPLASM PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL 2897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1139 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92874 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:26 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, FIRM AND LOBULATED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 40 X 35 X 20 MM, TAN AND -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1140 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92874 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:26 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1141 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92875 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 261.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:25 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- -COMPRESSION, VENTRAL,-PRESENT ENTIRE; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >APPEARANCE; THIN; VERIFIED AT OCCASIONALLY NOTED,-MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : >SKIN & PELAGE; YELLOW HAIR COAT; -MINERALIZATION, PELVIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED SPLEEN (SP) : >DISCHARGE; NASAL; RED IN COLOR; -PIGMENT, INCREASED,-MODERATE VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LARGE; LEFT: 8 X 5 X 3 MM (RIGHT IS 4 -ANGIECTASIS,-MODERATE NECROPSY: NOT APPLICABLE X 3 X 3 MM) -PIGMENT,-SLIGHT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; GREEN >UNREMARKABLE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1142 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92875 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 261.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:25 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 13 X 8 X 5 MM, RED, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE >UNREMARKABLE BROWN, ERODED AREA, 3 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER (CAP 21) URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -DISCHARGE-NASAL; DARK RED AND CRUSTED -STAINS - SKIN/HAIRCOAT; TOP OF HEAD: RED AND DRY -------------------------------------------- 2901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1143 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92875 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 261.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:25 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1144 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92876 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 353.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0292 .0083 % .0133 WEIGHT TAKEN RT ADRENAL (AD1) .0329 .0093 % .0150 WEIGHT TAKEN BRAIN (BR) 2.1997 .6223 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.3059 .3694 % .5937 WEIGHT TAKEN RT KIDNEY (KD1) 1.3705 .3877 % .6230 WEIGHT TAKEN LIVER (LI) 8.7983 2.4889 % 3.9998 WEIGHT TAKEN LUNG (LU) 1.6995 .4808 % .7726 WEIGHT TAKEN LF OVARY (OV0) .0538 .0152 % .0245 WEIGHT TAKEN RT OVARY (OV1) .0463 .0131 % .0210 WEIGHT TAKEN SPLEEN (SP) .5329 .1507 % .2423 WEIGHT TAKEN LF THYROID/PARA (TT0) .0101 .0029 % .0046 WEIGHT TAKEN RT THYROID/PARA (TT1) .0176 .0050 % .0080 WEIGHT TAKEN UTERUS (UT) .5771 .1633 % .2624 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 53; 10 X 10MM; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 2903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1145 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92876 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 353.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-FIBROSARCOMA,-NEOPLASM PRESENT SINGLE MASS, 10 X 10 X 5 MM, TAN, FIRM AND GELATINOUS; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (INGUINAL) NORMAL (CAP 21) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, INGUINAL (IN) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1146 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92876 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 353.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1147 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92877 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, PINPOINT AND -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / UP TO 2 MM; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-MODERATE SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -CONGESTION,-PRESENT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1148 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92877 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 2907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1149 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92877 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1150 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92878 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 510.6 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 10:17 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >MASS(ES); VENTRAL-THORACIC; WEEK 45; -HYPERTROPHY, HEPATOCELLULAR, 100 X 100MM; VERIFIED AT NECROPSY: PERIPORTAL,-SLIGHT OBSERVED KIDNEY (KD) : >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -NEPHROPATHY, CHRONIC PROGRESSIVE,- 60; 35 X 35MM; VERIFIED AT NECROPSY: MINIMAL OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, TUBULAR,-MINIMAL NECROPSY: OBSERVED SPLEEN (SP) : SPLEEN (SP) : -LARGE; 48 X 13 X 9 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 9 X 7 X 5 -DEGENERATION, CYSTIC / HEMORRHAGIC,- MM SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL 2909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1151 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92878 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 510.6 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 10:17 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 50 X 40 X 28 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN AND FIRM; ON SECTION -B-ADENOMA,-NEOPLASM PRESENT EXUDES A DARK RED, CREAMY SEMIFLUID; >NOTE:>MASS #2 IS THE FIBROADENOMA COLLECTED INTACT; REGIONAL (INGUINAL) NORMAL (CAP 21) -MASS #2; MID CERVICAL REGION, EXTENDING TO VENTRAL THORACIC REGION: SINGLE MASS, 95 X 82 X 45 MM, RED, DARK RED, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTIONS COLLECTED; REGIONAL LN (SEE MANDIBULAR) CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 10 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 8 X 3 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 2910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1152 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92878 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 510.6 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 10:17 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1153 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92879 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 312.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 12:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 69; 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT NOT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); RECTUM; WEEK 92; 15 X 15MM; -FOCUS, ALTERED HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED BASOPHILIC,-MODERATE >BEHAVIOR; ATAXIC; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM 2912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1154 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92879 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 312.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 12:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); PERIANAL REGION EXTENDING -CYST / GALACTOCELE,-PRESENT TOWARDS LEFT INGUINAL REGION: SINGLE -HYPERPLASIA, GLANDULAR,-MODERATELY MASS, 33 X 22 X 15 MM, RED, TAN AND SEVERE FIRM; ON SECTION EXUDES A TAN -B-ADENOMA,-NEOPLASM PRESENT SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1155 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92879 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 312.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 12:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1156 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92880 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:53 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0557 .0214 % .0289 WEIGHT TAKEN RT ADRENAL (AD1) .0540 .0208 % .0280 WEIGHT TAKEN BRAIN (BR) 1.9264 .7406 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.0967 .4216 % .5693 WEIGHT TAKEN RT KIDNEY (KD1) 1.1309 .4348 % .5871 WEIGHT TAKEN LIVER (LI) 8.6012 3.3069 % 4.4649 WEIGHT TAKEN LUNG (LU) 1.3026 .5008 % .6762 WEIGHT TAKEN LF OVARY (OV0) .0727 .0280 % .0377 WEIGHT TAKEN RT OVARY (OV1) .0804 .0309 % .0417 WEIGHT TAKEN SPLEEN (SP) .5075 .1951 % .2634 WEIGHT TAKEN LF THYROID/PARA (TT0) .0060 .0023 % .0031 WEIGHT TAKEN RT THYROID/PARA (TT1) .0076 .0029 % .0039 WEIGHT TAKEN UTERUS (UT) 1.1928 .4586 % .6192 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DARK BROWN FOCI, EACH IS 1 MM IN DIAMETER 2915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1157 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92880 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:53 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1158 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92881 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:08 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; MISSING; TEETH; VERIFIED SURFACE OF LEFT MEDIAN LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / AT NECROPSY: OBSERVED DARK RED AREA, 4 MM IN DIAMETER; EOSINOPHILIC CELL,-MINIMAL >MASS(ES); INGUINAL REGION-LEFT; WEEK SUNKEN WITH A STELLATE PATTERN (CAP -ANGIECTASIS,-SLIGHT 99; 20 X 20MM; VERIFIED AT NECROPSY: 23) OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); ATTACHED TO LEFT GLAND: -VACUOLATION, FOCAL,-SLIGHT SINGLE MASS, 5 X 5 X 4 MM, DARK RED, -HYPERTROPHY, CORTICAL CELL,-MODERATE BROWN, AND SOFT; COLLECTED INTACT; -DEGENERATION, CYSTIC / HEMORRHAGIC,- REGIONAL LN (SEE RENAL) MODERATE -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR >UNREMARKABLE FLUID FILLED CYST ON EACH, LEFT IS 4 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER 2917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1159 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92881 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:08 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR -HYPERPLASIA,-MODERATELY SEVERE ASPECT: SINGLE DARK RED AND SLIGHTLY RAISED AREA, 4 X 3 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 35 X 25 X 20 MM, DARK -HYPERPLASIA, GLANDULAR,-MODERATE RED, RED, GRAY, FIRM; SAME ON CUT -B-ADENOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; REGIONAL LN >NOTE:>MASS #2 IS THE FIBROADENOMA (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 22 X 12, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : LN, RENAL (RL) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 26) LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 2918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1160 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92881 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:08 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; RIGHT: DARK RED; -HEMORRHAGE,-PRESENT COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: MISSING -------------------------------------------- 2919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1161 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92881 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:08 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1162 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92882 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:14 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 69; 80 X 65MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -HEMORRHAGE,-SLIGHT OBSERVED -I-CARCINOMA,-FROM PITUITARY >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 100; 10 X 10MM; VERIFIED AT NECROPSY: -HYPERTROPHY, HEPATOCELLULAR, OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 7 X 4 MM (RIGHT IS 5 -ANGIECTASIS,-MODERATE X 4 X 3 MM) -HYPERTROPHY, CORTICAL CELL,-MODERATE -DIFFUSELY DARK; LEFT: DARK BROWN -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT 2921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1163 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92882 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:14 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 8 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, ACUTE,-SLIGHT MULTIPLE DARK BROWN AREAS, 3 X 1 MM RECTUM (RE) : >TISSUE MISSING MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 90 X 75 X 40 MM (129.0 -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT GRAMS), TAN, RED, LOBULATED, VASCULAR, -HYPERPLASIA, GLANDULAR,-MINIMAL AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) 2922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1164 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92882 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:14 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 30 X 15 MM, TAN AND FIRM; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1165 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92882 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:14 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1166 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >MASS(ES); INGUINAL REGION-RIGHT; WEEK CONSISTS PRIMARILY OF FOCAL 75; 20 X 20MM; VERIFIED AT NECROPSY: MINERALIZATION,-SLIGHT OBSERVED -INFLAMMATION, ACUTE,-SLIGHT >BEHAVIOR; ATAXIC; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT 2925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1167 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -VACUOLATION, FOCAL,-MINIMAL IS 4 MM IN DIAMETER) -ANGIECTASIS,-MODERATELY SEVERE -HYPERTROPHY, CORTICAL CELL,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); LEFT LOBE AND -INFILTRATE, MACROPHAGE, ALVEOLAR,- RIGHT CAUDAL LOBE, ALONG CAUDAL MINIMAL MARGINS: MULTIPLE TAN FOCI, UP TO 2 MM IN DIAMETER HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 11 X 9 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN, LOBULATED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, NEAR -EDEMA,-MODERATE PYLORIC VALVE: SINGLE TAN ERODED AREA, -INFLAMMATION, ACUTE,-SLIGHT 2 X 1 MM MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE 2926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1168 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 55 X 36 X 19 MM, DARK RED, GREY, TAN, LOBULATED AND SOFT; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID MATERIAL AND OTHER SECTIONS EXUDE A DARK RED, MUCOID SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CERVIX (CV) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT VAGINA (VA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR 2927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1169 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 2928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1170 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92884 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 13:36 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 65; 25 X 30MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -HEMORRHAGE,-MODERATE WEEK 74; 30 X 25MM POSTERIOR; VERIFIED LIVER (LI) : AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -NECROSIS, COAGULATIVE,-MINIMAL 79; 30 X 20MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK -INFILTRATE, MACROPHAGE, PIGMENTED,- 89; 20 X 15MM; VERIFIED AT NECROPSY: MINIMAL OBSERVED KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MINIMAL >EYES; PROTRUDING; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE NECROPSY: NOT OBSERVED SPLEEN (SP) : >SKIN & PELAGE; PALE; ENTIRE BODY; -PIGMENT, INCREASED,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY, >SKIN & PELAGE; SORE/SCAB; AXILLARY INCREASED,-MODERATE REGION-RIGHT; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : NOT OBSERVED -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-SLIGHT 2929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1171 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92884 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 13:36 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 9 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, GRAY, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,- 3 NEOPLASMS PRESENT SINGLE MASS, 45 X 30 X 10 MM, TAN, -CYST / GALACTOCELE,-PRESENT GRAY, YELLOW, AND CYSTIC; ON SECTION -B-FIBROADENOMA,-NEOPLASM PRESENT EXUDES A TAN AND YELLOW CREAMY >NOTE:>MASS #1 IS THE FIBROADENOMA SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 40 X 30 X 25 MM, RED, TAN VASCULAR, AND FIRM; SOME AREAS EXUDE A BROWN, CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) 2930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1172 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92884 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 13:36 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, YELLOW CREAMY SEMIFLUID FILLED CYSTS, UP TO 10 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #3; RIGHT INGUINAL REGION, POSTERIOR TO MF MASS #2: SINGLE MASS, 45 X 40 X 20 MM, RED, DARK RED, TAN, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL -MASS #4; LEFT AXILLARY REGION: SINGLE MASS, 40 X 15 X 10 MM, RED, DARK RED, BROWN, DARK BROWN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22); OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN, DRY, CRUSTED AREA, 20 X 10 MM; COLLECTED INTACT WITH MASS CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE LN, INGUINAL (IN) : >TISSUE MISSING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN 2931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1173 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92884 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 13:36 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND BROWN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1174 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92885 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 99; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / LOBE ALONG MARGIN: SINGLE TAN AREA, 5 EOSINOPHILIC CELL,-MINIMAL X 4 MM (CAP 22) -HYPERPLASIA, BILE DUCT,-MINIMAL -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERTROPHY, HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 3 X 2 PERIPORTAL,-MINIMAL MM; COLLECTED ON ROUTINE SECTIONS -ANGIECTASIS,-SLIGHT -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT 2933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1175 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92885 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 3 MM IN DIAMETER, RIGHT IS 8 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE >UNREMARKABLE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -HYPERPLASIA, GLANDULAR,-MODERATELY MULTIPLE TAN, CREAMY SEMIFLUID FILLED SEVERE CYSTS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1176 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92885 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ADIPOSE TISSUE SURROUNDING -M-LIPOSARCOMA,-NEOPLASM PRESENT LEFT KIDNEY: SINGLE MASS, 46 X 42 X 37 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH LEFT KIDNEY; REGIONAL LN (RENAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1177 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92885 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1178 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92886 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:50 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; AXILLARY BRAIN (BR) : BRAIN (BR) : REGION-LEFT; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; SWOLLEN; VENTRAL- LIVER (LI) : LIVER (LI) : ABDOMINAL; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED SURFACE OF LEFT LATERAL LOBE: MULTIPLE EOSINOPHILIC CELL,-MINIMAL >EYES; DISCOLORED/LIGHT RED; EYES; RED AREAS, UP TO 2 MM IN DIAMETER; -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE COLLECTED ON ROUTINE SECTION >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -DIFFUSELY LIGHT; BILATERAL: TAN -MINERALIZATION, PELVIC,-SLIGHT >SKIN & PELAGE; BLUE SKIN; VENTRAL- SPLEEN (SP) : ABDOMINAL; VERIFIED AT NECROPSY: NOT -HEMATOPOIESIS, EXTRAMEDULLARY, APPLICABLE INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT THYMUS (TH) : THYMUS (TH) : -GELATINOUS; DIFFUSE: DARK RED AND -EDEMA,-PRESENT SOFT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, FIRM, AND LOBULATED 2937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1179 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92886 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:50 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); VENTRAL -ACANTHOSIS/HYPERKERATOSIS,-MODERATE THORACIC REGION: SINGLE RED AND MOIST -ULCERATION,-SLIGHT ULCERATED AREA, 5 X 3 MM (CAP 21); -FIBROSIS,-MODERATE POSSIBLY CONSISTENT WITH (SORE, SCAB -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM LEFT AXILLARY REGION) NOTED IN PRESENT CLINICAL OBSERVATIONS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM; COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT, WITHIN LUMEN: SINGLE -DILATATION,-MINIMAL CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 24) -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT ADIPOSE TISSUE (AT) : -M-LIPOSARCOMA,-NEOPLASM PRESENT 2938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1180 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92886 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:50 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADIPOSE TISSUE (AT) : -CYST(S); INVOLVING ALL ADIPOSE TISSUE THROUGHOUT ABDOMINAL CAVITY: MULTIPLE CYSTS, SOME ARE FILLED WITH CLEAR AND OILY FLUID AND SOME ARE FILLED WITH RED AND OILY FLUID, UP TO 25 MM IN DIAMETER (CAP 22); POSSIBLY CONSISTENT WITH (SWOLLEN ABDOMEN) NOTED IN CLINICAL OBSERVATIONS DIAPHRAGM (DP) : DIAPHRAGM (DP) : -MASS(ES); VISCERAL SURFACE: MULTIPLE -I-LIPOSARCOMA,-NEOPLASM PRESENT MASSES, UP TO 4 MM IN DIAMETER, TAN FIRM, AND ADIPOSE TISSUE-LIKE (CAP 23); REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1181 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92886 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:50 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1182 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92887 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:02 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0376 .0136 % .0188 WEIGHT TAKEN RT ADRENAL (AD1) .0317 .0115 % .0159 WEIGHT TAKEN BRAIN (BR) 1.9947 .7235 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2699 .4606 % .6366 WEIGHT TAKEN RT KIDNEY (KD1) 1.2956 .4699 % .6495 WEIGHT TAKEN LIVER (LI) 9.7028 3.5193 % 4.8643 WEIGHT TAKEN LUNG (LU) 1.5519 .5629 % .7780 WEIGHT TAKEN LF OVARY (OV0) .0775 .0281 % .0389 WEIGHT TAKEN RT OVARY (OV1) .0731 .0265 % .0366 WEIGHT TAKEN SPLEEN (SP) .4733 .1717 % .2373 WEIGHT TAKEN LF THYROID/PARA (TT0) .0050 .0018 % .0025 WEIGHT TAKEN RT THYROID/PARA (TT1) .0075 .0027 % .0038 WEIGHT TAKEN UTERUS (UT) .7463 .2707 % .3741 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : KIDNEY (KD) : -CYST(S); RIGHT CORTICAL SURFACE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL EXTENDING TO CORTEX: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1183 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92887 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:02 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1184 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92888 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 545.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:52 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, MIDZONAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT / CENTRILOBULAR,-SLIGHT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL 2943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1185 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92888 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 545.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:52 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 5 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; LIGHT GREEN, -LUMEN, EXUDATE,-PRESENT CREAMY SEMIFLUID CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND CRUSTED -ANIMAL OBESE -------------------------------------------- 2944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1186 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92888 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 545.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:52 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1187 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92889 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 367.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:49 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0321 .0087 % .0168 WEIGHT TAKEN RT ADRENAL (AD1) .0277 .0075 % .0145 WEIGHT TAKEN BRAIN (BR) 1.9141 .5211 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1397 .3103 % .5954 WEIGHT TAKEN RT KIDNEY (KD1) 1.2457 .3392 % .6508 WEIGHT TAKEN LIVER (LI) 9.4841 2.5821 % 4.9549 WEIGHT TAKEN LUNG (LU) 1.9131 .5209 % .9995 WEIGHT TAKEN LF OVARY (OV0) .0753 .0205 % .0393 WEIGHT TAKEN RT OVARY (OV1) .0475 .0129 % .0248 WEIGHT TAKEN SPLEEN (SP) .4549 .1238 % .2377 WEIGHT TAKEN LF THYROID/PARA (TT0) .0284 .0077 % .0148 WEIGHT TAKEN RT THYROID/PARA (TT1) .0181 .0049 % .0095 WEIGHT TAKEN UTERUS (UT) .6258 .1704 % .3269 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFLAMMATION, SUBACUTE,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE 2946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1188 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92889 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 367.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:49 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT -B-ADENOMA,-NEOPLASM PRESENT POSTERIOR ASPECT: SINGLE DARK FOCUS, 2 -ANGIECTASIS,-PRESENT MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1189 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92889 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 367.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:49 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1190 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92890 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:55 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -MASS(ES); RIGHT CORTICAL SURFACE: -NEPHROPATHY, CHRONIC PROGRESSIVE,- SINGLE MASS, 5 MM IN DIAMETER, TAN AND MINIMAL FIRM; SAME ON CUT SURFACE; COLLECTED -M-CARCINOMA, TUBULAR CELL,-NEOPLASM INTACT; REGIONAL LN (RENAL) NORMAL PRESENT (CAP 22) SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 7 X 5 MM (LEFT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATELY X 4 X 3 MM) SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT 2949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1191 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92890 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:55 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT MID -HYPERPLASIA,-MODERATE ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER COLON (CO) : COLON (CO) : -DIVERTICULUM(A); NEAR JUNCTION OF -DIVERTICULUM,-PRESENT CECUM: SINGLE BROWN DIVERTICULUM, 5 X 5 X 3 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; -HYPERPLASIA, GLANDULAR,-MODERATE COLLECTED ON ROUTINE SECTION CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-PRESENT 2950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1192 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92890 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:55 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 2951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1193 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92890 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:55 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1194 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92891 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 591 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 8:08 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -RED FOCUS(I)/AREA(S); RIGHT MEDIAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE LOBE: SINGLE DARK RED FOCUS, 4 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >RESPIRATION; AUDIBLE; VERIFIED AT DIAMETER, SUNKEN WITH A STELLATE EOSINOPHILIC CELL,-SLIGHT NECROPSY: NOT APPLICABLE PATTERN; COLLECTED ON ROUTINE SECTION -ANGIECTASIS,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 2953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1195 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92891 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 591 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 8:08 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 21) MODERATE -HEMORRHAGE,-PRESENT CAVITY, THORACIC (TA) : CAVITY, THORACIC (TA) : -MASS(ES); ATTACHED TO PLEURA, BETWEEN -B-HIBERNOMA,-NEOPLASM PRESENT VERTEBRAE AND RIBS: SINGLE MASS, 20 X 10 X 10 MM, RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT WITH SPINAL CORDD; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 2954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1196 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92891 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 591 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 8:08 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1197 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92892 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 688.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; OBES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1198 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92892 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 688.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, RED, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 55 X 38 X 14 MM, RED, -HYPERPLASIA, GLANDULAR,-MODERATE DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 2957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1199 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92892 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 688.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1200 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 74; 10 X 10MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK BASOPHILIC,-MINIMAL 74; 40 X 40MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : THORACIC-LEFT; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : SPLEEN (SP) : -LARGE; 50 X 17 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-SLIGHT OVARY (OV) : -B-GRANULOSA/THECA CELL TUMOR,-NEOPLASM PRESENT 2959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1201 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, GRAY, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 20 X 10 X 10 MM, DARK -CYST / GALACTOCELE,-PRESENT RED, TAN, AND FIRM; SAME ON CUT >NOTE:>MASS #1 IS AN ENLARGED SURFACE; COLLECTED; REGIONAL LN (SEE SUBCUTANEOUS LYMPH NODE AND AXILLARY) GALACTOCELES -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 55 X 50 X 40 MM, DARK RED, TAN, AND FIRM; SOME SECTIONS CONTAIN A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND TAN ULCERATION, 30 MM IN DIAMETER; COLLECTED 2960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1202 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 8 X 5 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (RIGHT IS UP TO 7 X 6 X 3 MM) (CAP 23) MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 2961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1203 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1204 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92894 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 335.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .1249 .0372 % .0583 WEIGHT TAKEN RT ADRENAL (AD1) .0492 .0147 % .0230 WEIGHT TAKEN BRAIN (BR) 2.1413 .6384 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2732 .3796 % .5946 WEIGHT TAKEN RT KIDNEY (KD1) 1.3419 .4001 % .6267 WEIGHT TAKEN LIVER (LI) 10.7609 3.2084 % 5.0254 WEIGHT TAKEN LUNG (LU) 1.7755 .5294 % .8292 WEIGHT TAKEN LF OVARY (OV0) .0539 .0161 % .0252 WEIGHT TAKEN RT OVARY (OV1) .0463 .0138 % .0216 WEIGHT TAKEN SPLEEN (SP) .4753 .1417 % .2220 WEIGHT TAKEN LF THYROID/PARA (TT0) .0096 .0029 % .0045 WEIGHT TAKEN RT THYROID/PARA (TT1) .0161 .0048 % .0075 WEIGHT TAKEN UTERUS (UT) 1.4655 .4369 % .6844 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (FOR -HEMORRHAGE,-PRESENT COMPARISON, RIGHT IS 4 MM IN DIAMETER) 2963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1205 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92894 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 335.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -HEMORRHAGE,-PRESENT ESOPHAGUS (ES) : ESOPHAGUS (ES) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT >TISSUE MISSING TRIMMING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1206 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92894 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 335.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1207 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92895 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : 87; 45 X 40MM; VERIFIED AT NECROPSY: -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT 2966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1208 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92895 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 50 X 40 X 14 MM, RED, -HYPERPLASIA, GLANDULAR,-MODERATE DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1209 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92895 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 2968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1210 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92896 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 528.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:48 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 60 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-THORACIC; WEEK 104; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1211 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92896 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 528.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:48 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 3 MM, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); BILATERAL VENTRAL CERVICAL -M-CARCINOMA,-NEOPLASM PRESENT REGIONS: MULTIPLE MASSES, UP TO 10 X 9 -CYST / GALACTOCELE,-PRESENT X 9 MM, ALL ARE TAN AND FIRM; SAME ON -B-ADENOMA,-NEOPLASM PRESENT CUT SURFACES; REGIONAL LN (AXILLARY) >NOTE:>MASS #1 IS THE ADENOMA RIGHT (NORMAL) (CAP 23) LEFT (NOT IDENTIFIED) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 75 X 55 X 35 MM, DARK BROWN, TAN, SOFT, LOBULATED, AND VASCULAR; CUT SURFACE EXUDES A DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 24) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 2970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1212 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92896 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 528.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:48 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 2971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1213 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92897 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:46 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EYES; SQUINTED; PERI-ORBITAL; VERIFIED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL AT NECROPSY: NOT APPLICABLE MULTIPLE DARK RED AREAS, UP TO 4 X 3 -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / MM; COLLECTED ON ROUTINE SECTIONS EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1214 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92897 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:46 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, LOBULATED, AND FIRM MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT URINARY BLADDER (UB) : -DIVERTICULUM,-PRESENT UTERUS (UT) : -DILATATION,-SLIGHT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT GREEN MUCOID MATERIAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN 2973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1215 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92897 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:46 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1216 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92898 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 284.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:52 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0386 .0136 % .0185 WEIGHT TAKEN RT ADRENAL (AD1) .0332 .0117 % .0159 WEIGHT TAKEN BRAIN (BR) 2.0817 .7325 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) .9699 .3413 % .4659 WEIGHT TAKEN RT KIDNEY (KD1) 1.0336 .3637 % .4965 WEIGHT TAKEN LIVER (LI) 8.3705 2.9453 % 4.0210 WEIGHT TAKEN LUNG (LU) 1.6120 .5672 % .7744 WEIGHT TAKEN LF OVARY (OV0) .0858 .0302 % .0412 WEIGHT TAKEN RT OVARY (OV1) .0637 .0224 % .0306 WEIGHT TAKEN SPLEEN (SP) .4762 .1676 % .2288 WEIGHT TAKEN LF THYROID/PARA (TT0) .0083 .0029 % .0040 WEIGHT TAKEN RT THYROID/PARA (TT1) .0080 .0028 % .0038 WEIGHT TAKEN UTERUS (UT) .4686 .1649 % .2251 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1217 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92898 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 284.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:52 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1218 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92899 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 435.3 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 9:15 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0446 .0102 % .0206 WEIGHT TAKEN RT ADRENAL (AD1) .0404 .0093 % .0186 WEIGHT TAKEN BRAIN (BR) 2.1677 .4980 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.4659 .3368 % .6762 WEIGHT TAKEN RT KIDNEY (KD1) 1.5078 .3464 % .6956 WEIGHT TAKEN LIVER (LI) 13.0348 2.9944 % 6.0132 WEIGHT TAKEN LUNG (LU) 2.0543 .4719 % .9477 WEIGHT TAKEN LF OVARY (OV0) .0505 .0116 % .0233 WEIGHT TAKEN RT OVARY (OV1) .8172 .1877 % .3770 WEIGHT TAKEN SPLEEN (SP) .6569 .1509 % .3030 WEIGHT TAKEN LF THYROID/PARA (TT0) .0086 .0020 % .0040 WEIGHT TAKEN RT THYROID/PARA (TT1) .0095 .0022 % .0044 WEIGHT TAKEN UTERUS (UT) 1.0497 .2411 % .4842 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LEFT; VERIFIED AT NECROPSY: NOT -VACUOLATION, HEPATOCELLULAR,-MINIMAL OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL 2977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1219 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92899 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 435.3 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 9:15 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 8 MM IN DIAMETER THYMUS (TH) : -HEMORRHAGE,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-SLIGHT AND FIRM; COLLECTED ON ROUTINE SECTION -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1220 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92899 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 435.3 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 9:15 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1221 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92900 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/05/99 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >APPEARANCE; MISSING; TEETH; VERIFIED CONSISTS PRIMARILY OF FOCAL AT NECROPSY: OBSERVED MINERALIZATION,-SLIGHT BRAIN (BR) : BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- IS 3 MM IN DIAMETER) SEVERE OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, EACH IS 3 MM IN DIAMETER THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 5 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT FIRM 2980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1222 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92900 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/05/99 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CECUM (CE) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -HYPERPLASIA, LYMPHOPLASMACYTIC,- -LARGE; BILATERAL: EACH IS 8 X 4 X 2 MODERATELY SEVERE MM GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; MISSING, LEFT UPPER INCISOR -------------------------------------------- 2981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1223 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92900 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/05/99 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1224 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92901 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); RECTUM; WEEK 76; 10 X 10MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED SPLEEN (SP) : AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE >EYES; RED DISCHARGE; EYE-LEFT; ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: OBSERVED -ANGIECTASIS,-MODERATE >BEHAVIOR; ATAXIC; VERIFIED AT THYMUS (TH) : NECROPSY: NOT APPLICABLE -DEPLETION, LYMPHOCYTIC (GENERALLY >DISCHARGE; NASAL; RED IN COLOR; COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- VERIFIED AT NECROPSY: OBSERVED MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1225 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92901 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); RIGHT PERIANAL REGION: SINGLE GREEN SEMIFLUID FILLED CYST, 11 MM IN DIAMETER; COLLECTED; POSSIBLY CONSISTENT WITH MASS (RECTUM) NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN WALL: -DILATATION,-MINIMAL MULTIPLE CLEAR FLUID FILLED CYSTS; UP -B-POLYP, ENDOMETRIAL, STROMAL,- TO 3 MM IN DIAMETER NEOPLASM PRESENT -MASS(ES); ATTACHED TO ENDOMETRIUM OF DISTAL RIGHT HORN: SINGLE MASS, 27 X 7 X 7 MM, RED, DARK RED, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH UTERUS; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL: DRY RED MATERIAL -------------------------------------------- 2984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1226 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92901 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1227 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / SURFACE OF LEFT LATERAL LOBE: SINGLE EOSINOPHILIC CELL,-MINIMAL TAN AREA, 9 X 2 MM; COLLECTED ON -NECROSIS, COAGULATIVE,-SLIGHT ROUTINE SECTION -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 8 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1228 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE >NOTE:>SEE SLIDE #16 THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; VENTRAL CERVICAL REGION: -CYST / GALACTOCELE,-PRESENT TAN AND FIRM (CAP 21) -HYPERPLASIA, GLANDULAR,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PANCREAS,-PRESENT CAVITY, ABDOM (PC) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PANCREAS FOR DIAGNOSIS 2987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1229 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE OF COLON AND ILEUM: SINGLE MASS, 30 X 20 X 15 MM, RED, DARK RED, TAN, GRAY, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: YELLOW, RED AND MOIST -------------------------------------------- 2988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1230 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1231 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92903 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 349.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0438 .0125 % .0204 WEIGHT TAKEN RT ADRENAL (AD1) .0435 .0124 % .0202 WEIGHT TAKEN BRAIN (BR) 2.1518 .6150 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2498 .3572 % .5808 WEIGHT TAKEN RT KIDNEY (KD1) 1.2691 .3627 % .5898 WEIGHT TAKEN LIVER (LI) 11.8895 3.3980 % 5.5254 WEIGHT TAKEN LUNG (LU) 1.5288 .4369 % .7105 WEIGHT TAKEN LF OVARY (OV0) .0645 .0184 % .0300 WEIGHT TAKEN RT OVARY (OV1) .0745 .0213 % .0346 WEIGHT TAKEN SPLEEN (SP) .5819 .1663 % .2704 WEIGHT TAKEN LF THYROID/PARA (TT0) .0084 .0024 % .0039 WEIGHT TAKEN RT THYROID/PARA (TT1) .0099 .0028 % .0046 WEIGHT TAKEN UTERUS (UT) .7114 .2033 % .3306 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; SPLEEN (SP) : VERIFIED AT NECROPSY: OBSERVED -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT PRESENT AT EMBEDDING ADRENAL, MEDULLA (MA) : ADRENAL, MEDULLA (MA) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT PRESENT AT EMBEDDING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1232 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92903 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 349.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1233 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 70; 40 X 35MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 73; 30 X 35MM; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-SLIGHT >MASS(ES); AXILLARY REGION-LEFT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, 69; 55 X 65MM; VERIFIED AT NECROPSY: BASOPHILIC,-SLIGHT OBSERVED -NECROSIS, COAGULATIVE,-SLIGHT >WART LIKE LESION(S); MOUTH; WEEK 89; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK SPLEEN (SP) : 97; 30 X 20MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY, OBSERVED INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 X 5 X 4 -HYPERTROPHY, CORTICAL CELL,-SLIGHT MM -DEGENERATION, CYSTIC / HEMORRHAGIC,- -MOTTLED; BILATERAL: RED, BROWN AND MODERATE TAN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL 2992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1234 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : SKIN (SK) : -RAISED FOCUS(I)/AREA(S); LEFT UPPER -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE LIP: SINGLE RED, TAN, FIRM RAISED -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM AREA, 4 X 2 X 2 MM (CAP 23) PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT SINGLE MASS, 45 X 40 X 22 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT ABDOMINAL REGION: SINGLE MASS, 45 X 30 X 20 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED 2993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1235 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION EXTENDS LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 65 X 55 X 40 MM (130.6 GRAMS), RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 26,27); REGIONAL LN (SEE AXILLARY) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE TO 1 MM IN DIAMETER VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY DARK; BILATERAL: BROWN (CAP -HEMORRHAGE,-PRESENT 22) -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; LEFT: 12 X 6 X 4 MM (RIGHT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 4 X 3 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 2994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1236 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 2995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1237 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92905 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 250.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:02 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0460 .0184 % .0219 WEIGHT TAKEN RT ADRENAL (AD1) .0463 .0185 % .0221 WEIGHT TAKEN BRAIN (BR) 2.0984 .8394 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2015 .4806 % .5726 WEIGHT TAKEN RT KIDNEY (KD1) 1.2025 .4810 % .5731 WEIGHT TAKEN LIVER (LI) 7.9055 3.1622 % 3.7674 WEIGHT TAKEN LUNG (LU) 1.4232 .5693 % .6782 WEIGHT TAKEN LF OVARY (OV0) .1031 .0412 % .0491 WEIGHT TAKEN RT OVARY (OV1) .0740 .0296 % .0353 WEIGHT TAKEN SPLEEN (SP) .5258 .2103 % .2506 WEIGHT TAKEN LF THYROID/PARA (TT0) .0066 .0026 % .0031 WEIGHT TAKEN RT THYROID/PARA (TT1) .0153 .0061 % .0073 WEIGHT TAKEN UTERUS (UT) 1.2028 .4811 % .5732 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; SPLEEN (SP) : VERIFIED AT NECROPSY: OBSERVED -PIGMENT, INCREASED,-SLIGHT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 2996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1238 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92905 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 250.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:02 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 2997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1239 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92906 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >MASS(ES); AXILLARY REGION-RIGHT; WEEK CONSISTS PRIMARILY OF FOCAL 95; 40 X 30MM; VERIFIED AT NECROPSY: MINERALIZATION,-MODERATE OBSERVED BRAIN (BR) : BRAIN (BR) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT 95; 20 X 20MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 MM IN DIAMETER (LEFT -ANGIECTASIS,-SLIGHT IS 4 MM IN DIAMETER) -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-SEVERE 2998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1240 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92906 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 30 X 25 X 18 MM, TAN AND -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SOFT; ON SECTION EXUDES A TAN AND -HYPERPLASIA, GLANDULAR,-SLIGHT CREAMY SEMIFLUID; (CAP 22); REGIONAL LN (LEFT AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 47 X 35 X 25 MM, RED, DARK RED, TAN AND FIRM; SAME ON SURFACE; (CAP 24); REGIONAL LN (SEE AXILLARY) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: (CAP 23) -HEMORRHAGE,-PRESENT 2999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1241 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92906 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 3000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1242 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92906 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1243 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92907 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:17 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE RED AND DARK RED AREAS, UP TO -HYPERTROPHY, HEPATOCELLULAR, 4 X 3 MM; COLLECTED ON ROUTINE PERIPORTAL,-SLIGHT SECTIONS -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MODERATE IS 4 MM IN DIAMETER) -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -HYPERPLASIA, LYMPHORETICULAR,- MODERATELY SEVERE 3002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1244 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92907 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:17 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE TAN AND LOBULATED MASS, 15 X 13 X 6 MM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 25) LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM, APPEARS SWOLLEN -HYPERPLASIA,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE GRAY AREAS, UP TO 2 X 1 MM ILEUM (IL) : -HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE),-MODERATE SKIN (SK) : -B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN WITHIN WALL: -DILATATION,-MODERATELY SEVERE MULTIPLE CLEAR FLUID FILLED CYSTS; UP TO 6 MM IN DIAMETER 3003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1245 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92907 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:17 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE LN, INGUINAL (IN) : >TISSUE MISSING LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 22) MODERATE -HEMORRHAGE,-PRESENT CLITORAL GLAND (CL) : CLITORAL GLAND (CL) : -MASS(ES); INVOLVING ENTIRE ORGAN: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE MASS, 21 X 18 X 13 MM, RED, >NOTE:>SEE SKIN FOR DIAGNOSIS DARK RED, BLACK, AND SOFT; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1246 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92907 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:17 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1247 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92908 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:19 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 78; BRAIN (BR) : BRAIN (BR) : 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -FOCUS, ALTERED HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE BASOPHILIC,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -PIGMENT,-MINIMAL 3006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1248 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92908 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:19 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -METAPLASIA, OSSEOUS / CARTILAGINOUS,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL CERVICAL -M-CARCINOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 26 X 21 X 17 MM, -CYST / GALACTOCELE,-PRESENT RED, DARK RED, GREY, TAN AND SOFT; ON SECTION EXUDES A TAN, CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1249 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92908 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:19 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 3008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1250 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92908 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:19 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1251 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92909 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:06 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 98; 80 X 45MM; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -DIFFUSELY LIGHT; BILATERAL: TAN SLIGHT -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-MINIMAL 3010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1252 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92909 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:06 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -N-CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MF MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID INGUINAL REGION -M-CARCINOMA,-NEOPLASM PRESENT EXTENDING TO PERIANAL REGION: SINGLE -HYPERPLASIA, GLANDULAR,-MODERATE MASS, 120 X 50 X 35 MM (127.0 GRAMS), RED, DARK RED, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MF MASS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE 3011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1253 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92909 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:06 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -I-CARCINOMA,-FROM MAMMARY, FEMALE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP -N-CARCINOMA,-FROM MAMMARY, FEMALE 23) URETER (UE) : URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 1 MM >TISSUE MISSING IN DIAMETER (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -URINARY BLADDER-LARGE LUMEN; 40 X 25 X 20 MM -------------------------------------------- 3012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1254 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92909 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:06 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1255 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92910 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 387.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0472 .0122 % .0219 WEIGHT TAKEN RT ADRENAL (AD1) .0441 .0114 % .0205 WEIGHT TAKEN BRAIN (BR) 2.1542 .5559 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.3866 .3578 % .6437 WEIGHT TAKEN RT KIDNEY (KD1) 1.3904 .3588 % .6454 WEIGHT TAKEN LIVER (LI) 10.9820 2.8341 % 5.0979 WEIGHT TAKEN LUNG (LU) 1.7913 .4623 % .8315 WEIGHT TAKEN LF OVARY (OV0) .0259 .0067 % .0120 WEIGHT TAKEN RT OVARY (OV1) .0379 .0098 % .0176 WEIGHT TAKEN SPLEEN (SP) .5683 .1467 % .2638 WEIGHT TAKEN LF THYROID/PARA (TT0) .0124 .0032 % .0058 WEIGHT TAKEN RT THYROID/PARA (TT1) .0103 .0027 % .0048 WEIGHT TAKEN UTERUS (UT) 1.1855 .3059 % .5503 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1256 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92910 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 387.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK BROWN AREA, 5 X 2 MM -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 20) MODERATELY SEVERE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 3015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1257 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92910 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 387.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1258 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92911 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 357.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:05 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -VACUOLATION, HEPATOCELLULAR, SURFACE OF LEFT LATERAL LOBE: SINGLE PERIPORTAL,-MINIMAL DARK RED AREA, 9 X 6 MM; COLLECTED ON -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / ROUTINE SECTION EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL 3017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1259 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92911 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 357.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:05 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 25 X 17 X 10 MM, DARK -CYST / GALACTOCELE,-PRESENT RED, RED, TAN, FIRM, AND GELATINOUS; -HYPERPLASIA, GLANDULAR,-SLIGHT SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1260 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92911 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 357.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:05 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1261 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92912 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 704.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 80; 90 X 100MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ABDOMINAL; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, OBSERVED PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER -HYPERPLASIA, GRANULOSA CELL,-MODERATE 3020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1262 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92912 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 704.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : >TISSUE MISSING LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); EXTENDS FROM RIGHT INGUINAL -CYST / GALACTOCELE,-PRESENT TO LEFT INGUINAL REGION: SINGLE MASS, -B-FIBROADENOMA,-NEOPLASM PRESENT 115 X 75 X 30 MM (190.6 GRAMS), TAN, -HYPERPLASIA, GLANDULAR,-MINIMAL RED, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A TAN DRY CRUSTED AREA, 30 X 20 MM; COLLECTED INTACT WITH MASS VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -HYPERPLASIA, MUCINOUS,-MINIMAL CONTAINS A LIGHT GREEN SEMIFLUID MATERIAL 3021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1263 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92912 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 704.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1264 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92913 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 91; BRAIN (BR) : 50 X 45MM; VERIFIED AT NECROPSY: -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT ABDOMINAL; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 6 X 4 MM (LEFT IS 4 -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT X 4 X 3 MM) -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-SLIGHT 3023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1265 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92913 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN >UNREMARKABLE HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1266 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92913 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 55 X 35 X 30 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST ULCERATED AREA, 45 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1267 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92913 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1268 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92914 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 181.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.2302 3.9878 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1269 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92914 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 181.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1270 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92915 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 292.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 8:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- >EYES; RED DISCHARGE; EYES; VERIFIED AT MINIMAL NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SEVERE -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT 3029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1271 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92915 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 292.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 8:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 10 X 6 -B-ADENOMA,-NEOPLASM PRESENT MM, RED, DARK RED, TAN, GRAY, AND SOFT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CERVIX (CV) : CERVIX (CV) : -FIRM; DIFFUSE >UNREMARKABLE -LARGE; 10 X 6 X 5 MM VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE HINDFEET: SINGLE TAN AND MOIST AREA ON -ULCERATION,-SLIGHT EACH, EACH IS 4 X 3 X 3 MM; BOTH -ACANTHOSIS / HYPERKERATOSIS,-MODERATE COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1272 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92915 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 292.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 8:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1273 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92916 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/28/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 01/28/00 11:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 46; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 70; 10 X 10MM; VERIFIED AT NECROPSY: -HYPERPLASIA, BILE DUCT,-MINIMAL OBSERVED KIDNEY (KD) : >WART LIKE LESION(S); MOUTH; WEEK 75; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: OBSERVED SLIGHT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -MINERALIZATION, PELVIC,-SLIGHT 75; 50 X 40MM; VERIFIED AT NECROPSY: -MINERALIZATION, TUBULAR,-SLIGHT OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT >MASS(ES); INGUINAL REGION-LEFT; WEEK PANCREAS (PA) : 84; 20 X 20MM; VERIFIED AT NECROPSY: -ATROPHY, ACINAR,-MINIMAL OBSERVED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -LARGE; LEFT: 4 X 3 X 3 MM (RIGHT IS 4 -HYPERTROPHY, CORTICAL CELL,-SLIGHT RIGHT; VERIFIED AT NECROPSY: OBSERVED X 3 X 3 MM) -DEGENERATION, CYSTIC / HEMORRHAGIC,- >APPEARANCE; HEAD TILT; RIGHT; VERIFIED SLIGHT AT NECROPSY: NOT APPLICABLE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1274 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92916 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/28/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 01/28/00 11:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 5 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN, LOBULATED AND SOFT; PORTIONS ADHERED TO BRAIN WITH MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); ATTACHED TO LOWER -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM RIGHT LIP: SINGLE DRY, BROWN CRUSTED PRESENT AREA, 2 X 2 X 1 MM (CAP 21) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,- 3 NEOPLASMS PRESENT SINGLE TAN AND SOFT MASS, 16 X 11 X 7 -CYST / GALACTOCELE,-PRESENT MM; SAME ON CUT SURFACE (CAP 25); -B-FIBROADENOMA,-NEOPLASM PRESENT REGIONAL LN (AXILLARY) NORMAL (CAP 24) -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>MASS #1 IS THE FIBROADENOMA 3033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1275 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92916 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/28/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 01/28/00 11:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 21 X 15 X 9 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; ON SECTION EXUDES A DARK RED FLUID (CAP 26); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 31 X 25 X 14 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (INGUINAL) LEFT NOT IDENTIFIED, RIGHT IS NORMAL (CAP 23) -MASS #4; RIGHT INGUINAL REGION: SINGLE MASS, 58 X 43 X 28 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING >NOTE:>PIECE OF TISSUE THOUGHT TO BE LYMPH NODE IS ACTUALLY A PORTION OF THE MAMMARY TUMOR FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -B-FIBROMA,-NEOPLASM PRESENT 3034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1276 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92916 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/28/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 01/28/00 11:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DRY, DARK RED CRUSTED AREA, 6 X 5 X 3 MM; SURROUNDING TISSUE APPEARS SWOLLEN (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 3035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1277 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92916 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/28/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 01/28/00 11:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1278 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92917 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 523.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0691 .0132 % .0319 WEIGHT TAKEN RT ADRENAL (AD1) .0419 .0080 % .0194 WEIGHT TAKEN BRAIN (BR) 2.1639 .4133 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.5698 .2998 % .7254 WEIGHT TAKEN RT KIDNEY (KD1) 1.5702 .2999 % .7256 WEIGHT TAKEN LIVER (LI) 14.4167 2.7534 % 6.6624 WEIGHT TAKEN LUNG (LU) 1.8308 .3497 % .8461 WEIGHT TAKEN LF OVARY (OV0) .0734 .0140 % .0339 WEIGHT TAKEN RT OVARY (OV1) .1023 .0195 % .0473 WEIGHT TAKEN SPLEEN (SP) .6282 .1200 % .2903 WEIGHT TAKEN LF THYROID/PARA (TT0) .0112 .0021 % .0052 WEIGHT TAKEN RT THYROID/PARA (TT1) .0154 .0029 % .0071 WEIGHT TAKEN UTERUS (UT) 1.5030 .2871 % .6946 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1279 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92917 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 523.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); AT CRANIAL END: SINGLE -NECROSIS / INFLAMMATION, ADIPOSE MASS, 19 X 15 X 8 MM, TAN LOBULATED TISSUE,-PRESENT AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -CYST(S); TRIMMING COMMENT: ADRENAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- HAS A SINGLE CYST, 2 MM IN DIAMETER MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE WHITE AND CREAMY SEMIFLUID -HYPERPLASIA, GLANDULAR,-MODERATE FILLED CYSTS, UP TO 6 MM IN DIAMETER; -SECRETORY MATERIAL,-PRESENT COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HYPERPLASIA, LYMPHOPLASMACYTIC,- COLLECTED MODERATE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN 3038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1280 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92917 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 523.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1281 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92918 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/09/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 347.3 GRAMS DATE AND TIME OF NECROPSY: 09/09/99 14:24 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -CYST, BILIARY,-PRESENT NECROPSY: OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 9 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1282 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92918 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/09/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 347.3 GRAMS DATE AND TIME OF NECROPSY: 09/09/99 14:24 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1283 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92918 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/09/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 347.3 GRAMS DATE AND TIME OF NECROPSY: 09/09/99 14:24 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1284 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92919 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 234.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 9.0816 3.8714 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1285 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92919 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 234.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1286 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92920 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 299.2 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:55 PROSECTOR: DENNIS HOFFMAN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR, MULTIPLE RED AREAS, UP TO 3 X 4 MM; PERIPORTAL,-MODERATELY SEVERE COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : KIDNEY (KD) : -CYST(S); RIGHT, CORTICAL SURFACE: -MINERALIZATION, PELVIC,-MINIMAL SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 9 X 9 MM, -B-ADENOMA,-NEOPLASM PRESENT SOFT, RED, DARK RED, TAN AND BROWN 3045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1287 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92920 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 299.2 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:55 PROSECTOR: DENNIS HOFFMAN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 1 X 3 MM -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM; COLLECTED ON ROUTINE SECTION -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -CYST(S); ALL REGION, DIFFUSE: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 20) VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT LIGHT GREEN MUCOID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1288 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92920 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 299.2 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:55 PROSECTOR: DENNIS HOFFMAN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1289 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92921 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 299.9 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 14:22 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 48; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; ROUGH HAIR COAT; BASOPHILIC,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >SKIN & PELAGE; RED HAIR COAT; HEAD- -MINERALIZATION, PELVIC,-MINIMAL ENTIRE; VERIFIED AT NECROPSY: NOT SPLEEN (SP) : OBSERVED -PIGMENT, INCREASED,-SLIGHT >APPEARANCE; THIN; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -ANGIECTASIS,-MODERATELY SEVERE >EYES; RED DISCHARGE; EYES; VERIFIED AT THYMUS (TH) : NECROPSY: NOT OBSERVED -DEPLETION, LYMPHOCYTIC (GENERALLY >SKIN & PELAGE; SORE/SCAB; AXILLARY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- REGION-LEFT; VERIFIED AT NECROPSY: SLIGHT OBSERVED THYROID (TY) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE PITUITARY (PI) : PITUITARY (PI) : >RESPIRATION; LABORED; VERIFIED AT -MASS(ES); 15 X 8 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT NECROPSY: NOT APPLICABLE GREY AND SOFT SKIN (SK) : >UNREMARKABLE >NOTE:>SEE MAMMARY GLAND FOR DIAGNOSIS 3048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1290 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92921 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 299.9 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 14:22 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 20 X 18 MM, RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN IS BLACK AND CRUSTED; COLLECTED WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 20) MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1291 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92921 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 299.9 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 14:22 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1292 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92922 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:53 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0303 .0067 % .0136 WEIGHT TAKEN RT ADRENAL (AD1) .0315 .0069 % .0141 WEIGHT TAKEN BRAIN (BR) 2.2274 .4896 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.3199 .2902 % .5926 WEIGHT TAKEN RT KIDNEY (KD1) 1.4231 .3128 % .6389 WEIGHT TAKEN LIVER (LI) 11.3904 2.5039 % 5.1138 WEIGHT TAKEN LUNG (LU) 1.6016 .3521 % .7190 WEIGHT TAKEN LF OVARY (OV0) .0896 .0197 % .0402 WEIGHT TAKEN RT OVARY (OV1) .0890 .0196 % .0400 WEIGHT TAKEN SPLEEN (SP) .6828 .1501 % .3065 WEIGHT TAKEN LF THYROID/PARA (TT0) .0105 .0023 % .0047 WEIGHT TAKEN RT THYROID/PARA (TT1) .0145 .0032 % .0065 WEIGHT TAKEN UTERUS (UT) .7457 .1639 % .3348 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT 3051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1293 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92922 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:53 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -HEMORRHAGE,-PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1294 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92922 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:53 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92923 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0305 .0081 % .0143 WEIGHT TAKEN RT ADRENAL (AD1) .0273 .0073 % .0128 WEIGHT TAKEN BRAIN (BR) 2.1318 .5691 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.0985 .2932 % .5153 WEIGHT TAKEN RT KIDNEY (KD1) 1.1508 .3072 % .5398 WEIGHT TAKEN LIVER (LI) 9.0597 2.4185 % 4.2498 WEIGHT TAKEN LUNG (LU) 1.3671 .3649 % .6413 WEIGHT TAKEN LF OVARY (OV0) .0361 .0096 % .0169 WEIGHT TAKEN RT OVARY (OV1) .0412 .0110 % .0193 WEIGHT TAKEN SPLEEN (SP) .5812 .1552 % .2726 WEIGHT TAKEN LF THYROID/PARA (TT0) .0102 .0027 % .0048 WEIGHT TAKEN RT THYROID/PARA (TT1) .0114 .0030 % .0053 WEIGHT TAKEN UTERUS (UT) 1.1680 .3118 % .5479 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL- LIVER (LI) : THORACIC-RIGHT; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL -INFLAMMATION, SUBACUTE,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE STOMACH, GL (ST) : -EDEMA,-MINIMAL -INFLAMMATION, ACUTE,-MINIMAL 3054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1296 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92923 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL LUMBAR -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL REGION: SINGLE RED AND TAN AREA, 12 X -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 8 MM (CAP 20) -ULCERATION,-MODERATE MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT CERVIX (CV) : CERVIX (CV) : -MASS(ES); RIGHT SIDE OF SEROSAL -CYST, SQUAMOUS,-PRESENT SURFACE, SINGLE MASS, 4 MM IN DIAMETER, TAN AND FIRM, SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1297 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92923 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1298 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92924 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); LIMB-HIND-LEFT; WEEK 83; 80 BRAIN (BR) : BRAIN (BR) : X 60MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT, CORTEX: -DEGENERATION, CYSTIC / HEMORRHAGIC,- TWO BROWN SLIGHTLY RAISED AREAS, EACH MODERATE IS 2 MM IN DIAMETER -PIGMENT,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT 3057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1299 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92924 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : >NOTE:>THIGH MUSCLE: ROUTINE SECTION TAKEN FROM RIGHT THIGH, DUE TO MASS NERVE, SCIATIC (SN) : NERVE, SCIATIC (SN) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT >TISSUE MISSING SIDE DUE TO MASS PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GREY, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT >NOTE:>ALSO SEE CLITORAL GLAND SEE SKIN FOR DIAGNOSIS OF MASS 3058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1300 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92924 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL AREA, EXTENDING OVER LEFT FLANK: SINGLE MASS, 70 X 70 X 55 MM, RED, DARK RED, TAN, LOBULATED, AND CYSTIC; SOME SECTIONS EXUDE A DARK RED FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21); OVERLYING SKIN HAS A SINGLE BLACK CRUSTED AREA, 40 X 25; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT CLITORAL GLAND (CL) : -ECTASIA, DUCTAL,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1301 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92924 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1302 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92925 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:28 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 84; 80 X 80MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL; WEEK 92; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 15 X 15MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, OBSERVED PERIPORTAL,-SLIGHT >MASS(ES); AXILLARY REGION-RIGHT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 99; 20 X 20MM; VERIFIED AT NECROPSY: EOSINOPHILIC CELL,-SLIGHT OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT >SKIN & PELAGE; SORE/SCAB; VENTRAL- -FIBROSIS, PERIBILIARY,-SLIGHT ABDOMINAL; VERIFIED AT NECROPSY: -HYPERTROPHY, HEPATOCELLULAR, OBSERVED PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 3061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1303 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92925 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:28 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT -HYPERPLASIA,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN LINEAR AREAS, UP TO 3 X 1 MM MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -B-ADENOMA,- 2 NEOPLASMS PRESENT 3062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1304 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92925 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:28 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: >NOTE:>MASS #1 IS AN ADENOMA MASS #3 IS SINGLE MASS, 25 X 15 X 10 MM, RED, A FIBROADENOMA MASS #2 CONTAINS TAN, AND SOFT; ON SECTION EXUDES A TAN BOTH FIBROADENOMA AND ADENOMA CASEOUS SEMIFLUID (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 35 X 20 X 10 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; MID INGUINAL REGION EXTENDING TO PERIANAL REGION: SINGLE MASS, 110 X 85 X 30 MM (155.0 GRAMS), RED, DARK RED, AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS MULTIPLE MOIST BROWN ERODED AREAS, UP TO 25 MM IN DIAMETER; COLLECTED WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; RIGHT; COLLECTED -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1305 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92925 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:28 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1306 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92926 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 203.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:34 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.4195 3.6553 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1307 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92926 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 203.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:34 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1308 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92927 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:15 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 88; 40 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -MINERALIZATION,-MINIMAL OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 91; 80 X 50MM MASSES APPEAR TO HAVE -VACUOLATION, HEPATOCELLULAR, GROWN TOGETHER; VERIFIED AT NECROPSY: PERIPORTAL,-SLIGHT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >MASS(ES); INGUINAL REGION-RIGHT; WEEK EOSINOPHILIC CELL,-MINIMAL 94; 25 X 20MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT >SKIN & PELAGE; BLACK SKIN; VENTRAL- -FIBROSIS, PERIBILIARY,-SLIGHT THORACIC; VERIFIED AT NECROPSY: KIDNEY (KD) : KIDNEY (KD) : OBSERVED -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); INGUINAL REGION-LEFT; WEEK -DIFFUSELY DARK; BILATERAL: DARK BROWN MINIMAL 105; 15 X 15MM; VERIFIED AT NECROPSY: -DILATATION, PELVIC,-SEVERE OBSERVED -DEGENERATION TUBULAR,-SLIGHT SPLEEN (SP) : SPLEEN (SP) : -LARGE; 57 X 15 X 8 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 7 X 7 MM (RIGHT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATELY X 3 X 3 MM) SEVERE -DIFFUSELY RED; LEFT: DARK RED -DEGENERATION, CYSTIC / HEMORRHAGIC,- SEVERE -PIGMENT,-MINIMAL 3067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1309 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92927 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:15 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 5 MM; APPEARS SWOLLEN -HYPERPLASIA,-MODERATE -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE MASS, 70 X 35 X 20 MM, RED, MODERATELY SEVERE DARK RED, TAN, GREY, LOBULATED AND -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT FIRM; SAME ON CUT SURFACE; OVERLYING -HYPERPLASIA, GLANDULAR,-SLIGHT SKIN HAS A SINGLE ULCERATED AREA, 50 X -FIBROSIS,-MODERATELY SEVERE 30 MM; COLLECTED INTACT WITH MASS (CAP >NOTE:>MASS #1 CONSISTS OF INFLAMMATION 22); REGIONAL LN (SEE AXILLARY) AND FIBROSIS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 3068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1310 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92927 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:15 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 50 X 50 X 35 MM, RED, TAN, GREY, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #3; RIGHT INGUINAL REGION AND LEFT PERIANAL REGION: TWO MASSES, RIGHT INGUINAL IS 40 X 25 X 20 MM, LEFT PERIANAL IS 25 X 20 X 15 MM, BOTH ARE RED, TAN, GREY AND FIRM; BOTH ARE SAME ON CUT SURFACE; RIGHT INGUINAL COLLECTED; REGIONAL LN (LEFT INGUINAL) NORMAL (CAP 23); RIGHT NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 10 X 8 X 3 -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE MM (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -GI TRACT-DARK FLUID CONTENT; DUODENUM, JEJUNUM, ILEUM, CECUM AND COLON: FILLED WITH A DARK GREEN SEMIFLUID MATERIAL -------------------------------------------- 3069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1311 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92927 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:15 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 3070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1312 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92928 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 460.4 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >EXCRETION; FEW FECES; VERIFIED AT CONSISTS PRIMARILY OF FOCAL NECROPSY: NOT APPLICABLE MINERALIZATION,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFLAMMATION, ACUTE,-MINIMAL NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >BEHAVIOR; ATAXIC; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1313 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92928 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 460.4 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE ESOPHAGUS (ES) : -HYPERKERATOSIS,-MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 10 X 8 -B-ADENOMA,-NEOPLASM PRESENT MM, DARK RED, RED, TAN AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT RED ERODED AREA, 10 X 4 MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; LEFT: RED AND TAN; COLLECTED -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 3072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1314 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92928 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 460.4 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1315 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92929 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 279.4 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:55 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -------------------------------------------- 3074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1316 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92929 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 279.4 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:55 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1317 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92930 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0457 .0072 % .0214 WEIGHT TAKEN RT ADRENAL (AD1) .0386 .0061 % .0180 WEIGHT TAKEN BRAIN (BR) 2.1404 .3388 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.8673 .2956 % .8724 WEIGHT TAKEN RT KIDNEY (KD1) 1.9836 .3140 % .9267 WEIGHT TAKEN LIVER (LI) 18.5142 2.9304 % 8.6499 WEIGHT TAKEN LUNG (LU) 1.5595 .2468 % .7286 WEIGHT TAKEN LF OVARY (OV0) .0605 .0096 % .0283 WEIGHT TAKEN RT OVARY (OV1) .0605 .0096 % .0283 WEIGHT TAKEN SPLEEN (SP) .6350 .1005 % .2967 WEIGHT TAKEN LF THYROID/PARA (TT0) .0206 .0033 % .0096 WEIGHT TAKEN RT THYROID/PARA (TT1) .0220 .0035 % .0103 WEIGHT TAKEN UTERUS (UT) .9256 .1465 % .4324 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; DORSAL- BRAIN (BR) : BRAIN (BR) : THORACIC-RIGHT; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED >NOTE:>SOME EXTENSION OF THE PITUITARY MASS INTO WHAT APPEARS TO BE A RECESS OF THE PIA MATER. THIS WAS NOT INTERPRETED TO BE ACTUAL INVASION OF THE BRAIN. LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT 3076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1318 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92930 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MODERATE -MINERALIZATION, TUBULAR,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 8 X 7 X 5 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL LUMBAR: -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT SINGLE TAN, DRY CRUSTED AREA, 23 X 7 -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MM (CAP 21) -ULCERATION,-SLIGHT -LIGHT AREA(S)/FOCUS(I); MID DORSAL -HEMORRHAGE,-PRESENT LUMBAR REGION: SINGLE TAN AREA, 25 X 7 -DEGENERATION, MUSCLE, SUBCUTANEOUS,- MM (CAP 22) SLIGHT 3077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1319 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92930 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY SEMIFLUID FILLED -SECRETORY MATERIAL,-PRESENT CYSTS, UP TO 12 X 10 X 8 MM (CAP 20) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1320 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92930 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1321 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92931 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/22/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/22/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 69; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -CONGESTION,-PRESENT -VACUOLATION, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -CONGESTION,-PRESENT 3080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1322 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92931 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/22/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/22/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 15 X 10 X 8 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 3081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1323 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92931 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/22/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/22/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1324 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92932 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 285 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 01/29/99 15:03 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 18; 45 X 35MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; PERIPORTAL,-MODERATE WEEK 25; 50 X 50MM; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -REGENERATION, TUBULAR EPITHELIUM,- NECROPSY: OBSERVED MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -VACUOLATION, TUBULAR EPITHELIUM,- NECROPSY: NOT APPLICABLE MINIMAL >SKIN & PELAGE; PALE; ENTIRE BODY; -KARYOMEGALY, TUBULAR CELL,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE SPLEEN (SP) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HEMATOPOIESIS, EXTRAMEDULLARY, ABDOMINAL-LEFT; VERIFIED AT NECROPSY: INCREASED,-MODERATE OBSERVED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 MM IN -ANGIECTASIS,-MODERATE DIAMETER ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE 3083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1325 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92932 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 285 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 01/29/99 15:03 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -DARK FOCUS(I)/AREA(S); MID POSTERIOR -ANGIECTASIS,-PRESENT ASPECT: SINGLE GREY FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); ENTIRE ABDOMINAL REGION, -M-CARCINOMA,-NEOPLASM PRESENT EXTENDING TO VULVA AND LEFT HIND LEG: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 90 X 50 X 30 MM, RED, -INFLAMMATION, CHRONIC-ACTIVE,- DARK RED, TAN, BROWN, FIRM, AND MODERATELY SEVERE LOBULATED; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK BROWN SEMIFLUID; COLLECTED; OVERLYING SKIN HAS MULTIPLE DARK RED AND BROWN ABRADED AREAS, UP TO 40 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NORMAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; TAN AND MUCOID -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : LN, INGUINAL (IN) : >NOTE:>INGUINAL LYMPH NODE THAT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,- REGIONAL TO MAMMARY GLAND MASS MODERATE IS COLLECTED (CAP 20) 3084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1326 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92932 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 285 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 01/29/99 15:03 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1327 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK EYE (EY) : 64; 50 X 50MM; VERIFIED AT NECROPSY: -INFLAMMATION, ACUTE,-SLIGHT OBSERVED BRAIN (BR) : BRAIN (BR) : >MASS(ES); INGUINAL REGION-RIGHT; WEEK -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE 74; 70 X 70MM; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; MALOCCLUSION; VERIFIED AT MULTIPLE AREAS, UP TO 5 X 4 MM; BASOPHILIC,-MINIMAL NECROPSY: NOT OBSERVED COLLECTED ON ROUTINE SECTIONS -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HYPERPLASIA, BILE DUCT,-SLIGHT ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; BLACK SKIN; VENTRAL- MODERATE ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SEVERE NOT APPLICABLE -MINERALIZATION, TUBULAR,-MODERATE >EXCRETION; NO FECES; VERIFIED AT -HYPERPLASIA, TRANSITIONAL CELL,- NECROPSY: NOT APPLICABLE MODERATE >RESPIRATION; LABORED; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY, >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; INCREASED,-SEVERE VERIFIED AT NECROPSY: NOT APPLICABLE PANCREAS (PA) : >EYES; PALE; EYES; VERIFIED AT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -LARGE; LEFT: 8 X 5 X 5 MM (RIGHT IS 5 -HYPERTROPHY, CORTICAL CELL,-SEVERE X 4 X 3 MM) 3086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1328 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- SEVERE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,- SLIGHT -MINERALIZATION, SEPTAL,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATELY SEVERE TRACHEA (TR) : -EXUDATE, LUMINAL,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM -B-ADENOMA,-NEOPLASM PRESENT -MOTTLED; BROWN, DARK BROWN AND TAN -CYST,-PRESENT 3087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1329 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT DARK RED ULCERATED AREA, 4 X 2 MM -MINERALIZATION, GLANDULAR MUCOSA,- MODERATELY SEVERE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 50 X 40 X 25 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM AND LOBULATED; SAME ON CUT -B-ADENOMA,-NEOPLASM PRESENT SURFACE; SOME SECTIONS EXUDE A TAN >NOTE:>MASS 2 IS THE FIBROADENOMA CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION, EXTENDING TO MID VENTRAL ABDOMINAL REGION: SINGLE MASS, 70 X 70 X 25 MM, DARK BROWN, BROWN, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; ON SECTION EXUDES A DARK BROWN FLUID MATERIAL; OVERLYING SKIN HAS MULTIPLE DRY DARK BROWN CRUSTED AREAS, UP TO 6 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT >NOTE:>CAUSE OF DEATH IS THE ADENOMA 3088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1330 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 22) MODERATE LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; RIGHT: 15 X 8 X 5 MM (LEFT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,- 5 X 3 X 2 MM) (CAP 21) MODERATELY SEVERE -ABSCESS,-MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST, BROWN MATERIAL -------------------------------------------- 3089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1331 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1332 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92934 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 327.4 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -LIPIDOSIS, SUBCAPSULAR (TENSION AT NECROPSY: NOT APPLICABLE LIPIDOSIS),-SLIGHT >APPEARANCE; THIN; VERIFIED AT SPLEEN (SP) : NECROPSY: OBSERVED -PIGMENT, INCREASED,-SLIGHT >BEHAVIOR; ATAXIC; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -ATROPHY, ACINAR,-SLIGHT >RESPIRATION; LABORED; VERIFIED AT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- NECROPSY: NOT APPLICABLE MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3091 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1333 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92934 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 327.4 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; RIGHT: UP TO 4 X 4 X 3 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (LEFT IS UP TO 3 X 2 X 2 MM) (CAP 21) MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DRY, RED MATERIAL -------------------------------------------- 3092 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1334 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92934 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 327.4 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3093 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1335 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92935 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 356.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 11:11 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYE-RIGHT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >BEHAVIOR; ATAXIC; VERIFIED AT -RED FOCUS(I)/AREA(S); LEFT LATERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE LOBE: SINGLE DARK RED AREA, 5 MM IN -VACUOLATION, HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT DIAMETER; COLLECTED ON ROUTINE SECTION PERIPORTAL,-MINIMAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 13 X 10 X 10 MM (LEFT -DEGENERATION, CYSTIC / HEMORRHAGIC,- IS 4 X 3 X 3 MM) SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT 3094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1336 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92935 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 356.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 11:11 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -DILATATION,-SLIGHT -MASS(ES); MID RIGHT HORN: SINGLE -B-LEIOMYOMA,-NEOPLASM PRESENT PEDUNCULATED MASS, 9 X 6 X 6 MM, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 21) VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE 3095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1337 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92935 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 356.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 11:11 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND MOIST -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1338 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92936 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:30 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 63; 50 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, SOFT, AND LOBULATED STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,- DARK BROWN ERODED AREAS, UP TO 3 MM IN MODERATELY SEVERE DIAMETER -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 3097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1339 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92936 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:30 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 55 X 35 X 22 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM, AND LOBULATED; SAME ON CUT -HYPERPLASIA, GLANDULAR,-SLIGHT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION FOLLOWING BLOOD COLLECTION -------------------------------------------- 3098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1340 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92936 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:30 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1341 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92937 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 270.4 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL; WEEK 61; -COMPRESSION, VENTRAL,-PRESENT 15 X 15MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -MINERALIZATION, PELVIC,-SLIGHT >MASS(ES); AXILLARY REGION-LEFT; WEEK SPLEEN (SP) : 64; VERIFIED AT NECROPSY: OBSERVED -PIGMENT, INCREASED,-SLIGHT >APPEARANCE; THIN; VERIFIED AT PANCREAS (PA) : NECROPSY: OBSERVED -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- >APPEARANCE; HUNCHED; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, FOCAL,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 3100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1342 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92937 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 270.4 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION AND CAP 20; POSSIBLY CONSISTENT WITH MASSES NOTED IN CLINICAL OBSERVATIONS CERVIX (CV) : >TISSUE MISSING VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY -------------------------------------------- 3101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1343 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92937 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 270.4 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1344 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92938 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 650.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:32 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; -NECROSIS, INDIVIDUAL HEPATOCYTE,- VERIFIED AT NECROPSY: OBSERVED MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 5 X 5 MM (LEFT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATELY X 4 X 4 MM) SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, RED, DARK -HYPERPLASIA,-MODERATELY SEVERE RED, GREY, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); MID PERINEAL REGION: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY SEMIFLUID FILLED -HYPERPLASIA, GLANDULAR,-MINIMAL CYSTS, UP TO 15 X 10 MM (CAP 22) 3103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1345 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92938 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 650.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:32 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SINGLE RED, TAN, AND MOIST AREA ON -ULCERATION,-SEVERE EACH, LEFT IS 5 X 3 MM, RIGHT IS 17 X -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT 13 X 3 MM, SURROUNDING TISSUE APPEARS SWOLLEN; BOTH COLLECTED ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -GELATINOUS; TISSUE SURROUNDING BOTH >NOT REQUIRED TO BE EXAMINED FOR ANIMAL UTERINE HORNS, AND BOTH KIDNEYS: CLEAR >NOTE:>SEE PERITONEAL CAVITY FOR AND YELLOW (CAP 23) DIAGNOSIS OF MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 3104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1346 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92938 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 650.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:32 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1347 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92939 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 47; 30 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL WEEK 63; 15 X 15MM; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -MINERALIZATION, TUBULAR,-MINIMAL THORACIC; VERIFIED AT NECROPSY: SPLEEN (SP) : OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, >MASS(ES); AXILLARY REGION-LEFT; WEEK INCREASED,-MODERATE 58; 30 X 30MM; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -N-CARCINOMA,-FROM MAMMARY, FEMALE 3106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1348 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92939 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); TRIMMING COMMENT: RIGHT CRANIAL LOBE: SINGLE TAN AREA, 3 X 4 MM; TRIMMED IN BLOCK 11 WITH ROUTINE SECTIONS PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT; PORTION COLLECTED ON BRAIN MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT SHOULDER REGION: -M-CARCINOMA,- 3 NEOPLASMS PRESENT SINGLE MASS, 60 X 40 X 25 MM, TAN, -CYST / GALACTOCELE,-PRESENT RED, LOBULATED, FIRM AND VASCULAR; -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SAME ON CUT SURFACE; SKIN OVERLYING MASS HAS DARK RED AND BROWN ULCERATED AREA, 30 X 20 MM; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 35 X 30 X 20 MM, TAN, DARK RED, RED AND LOBULATED; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN GELATINOUS SEMISOLID MATERIAL; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -MASS #3; RIGHT VENTRAL ABDOMINAL: SINGLE MASS, 30 X 30 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1349 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92939 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL INADVERTENTLY SACRIFICED WITH SODIUM PENTOBARBITAL -------------------------------------------- 3108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1350 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92939 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1351 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92940 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 587.6 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 13:31 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : 50; 100 X 90MM; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MINIMAL >MASS(ES); INGUINAL REGION-LEFT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, 73; 60 X 70MM; VERIFIED AT NECROPSY: BASOPHILIC,-MINIMAL OBSERVED SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 5 X 5 MM (RIGHT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATE X 3 X 3 MM) -DEGENERATION, CYSTIC / HEMORRHAGIC,- -IRREGULAR SHAPE; LEFT: LOBULATED SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER 3110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1352 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92940 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 587.6 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 13:31 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE GREY, INDISTINCT AREAS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGIONAL: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 80 X 61 X 55 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT DARK RED, GREY, TAN, LOBULATED, -B-ADENOMA,-NEOPLASM PRESENT VASCULAR, AND FIRM; SOME SECTIONS >NOTE:>MASS #3 IS THE ADENOMA EXUDE A TAN, CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 128 X 91 X 73 MM (199.0 GRAMS), RED, DARK RED, GREY, TAN, VASCULAR, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID MATERIAL; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #3; LEFT LATERAL THORACIC REGION: SINGLE MASS, 15 X 11 X 9 MM, DARK BROWN, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; LEFT: RED AND TAN (CAP 21) -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1353 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92940 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 587.6 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 13:31 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1354 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92941 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:05 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.4862 4.0494 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1355 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92941 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:05 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1356 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92942 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 11; 85 X 80MM; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,- >SKIN & PELAGE; SORE/SCAB; DORSAL; MODERATE VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL WEEK 50; 35 X 25MM; VERIFIED AT -MINERALIZATION, TUBULAR,-MINIMAL NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 12 X 17 X 75 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: -HEMORRHAGE,-PRESENT MULTIPLE RED FOCI, PINPOINT AND UP TO -INFILTRATE, MACROPHAGE, ALVEOLAR,- 1 MM IN DIAMETER MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT 3115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1357 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92942 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: >NOTE:>MASS #1 IS THE FIBROADENOMA SINGLE MASS, 23 X 28 X 40 MM, FIRM, TAN, WHITE AND LOBULATED; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 50 X 60 X 70 MM, FIRM, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE; OVERLYING SKIN HAS A 35 X 40 MM DARK BROWN, DRY CRUSTED AREA; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1358 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92942 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1359 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -COMPRESSION, VENTRAL,-PRESENT 92; 20 X 20MM; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -CYST(S); VISCERAL SURFACE OF RIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT MEDIAN LOBE: SINGLE CLEAR FLUID FILLED -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: OBSERVED CYST, 1 MM IN DIAMETER; COLLECTED ON -HYPERTROPHY, HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT ROUTINE SECTION PERIPORTAL,-SLIGHT NECROPSY: NOT APPLICABLE -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 MM IN DIAMETER MM -HYPERTROPHY, CORTICAL CELL,-MODERATE (LEFT IS 5 X 3 X 2 MM) -DEGENERATION, CYSTIC / HEMORRHAGIC,- -RED FOCUS(I)/AREAS(I); RIGHT: SINGLE MODERATELY SEVERE DARK RED AREA, 7 X 5 MM -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; GREEN -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT 3118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1360 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 9 X 4 X 3 MM (LEFT IS 5 >UNREMARKABLE X 3 X 2 MM) -DIFFUSELY LIGHT; BILATERAL: TAN PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, BROWN, TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 30 X 25 X 20 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT TAN, LOBULATED, AND FIRM; SAME ON CUT >NOTE:>FIBROADENOMA ALSO ON SLIDE #21 SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM (CAP 21) UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE >UNREMARKABLE CERVIX (CV) : CERVIX (CV) : -LARGE; 10 MM IN DIAMETER -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE 3119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1361 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE-THIRD -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE OF TAIL: MULTIPLE BROWN DRY CRUSTED -ACANTHOSIS / HYPERKERATOSIS,-MODERATE AREAS, UP TO 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1362 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 3121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1363 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92944 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 346.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:16 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0428 .0124 % .0193 WEIGHT TAKEN RT ADRENAL (AD1) .0403 .0116 % .0182 WEIGHT TAKEN BRAIN (BR) 2.2158 .6395 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2156 .3508 % .5486 WEIGHT TAKEN RT KIDNEY (KD1) 1.2941 .3735 % .5840 WEIGHT TAKEN LIVER (LI) 10.8619 3.1347 % 4.9020 WEIGHT TAKEN LUNG (LU) 1.4298 .4126 % .6453 WEIGHT TAKEN LF OVARY (OV0) .0969 .0280 % .0437 WEIGHT TAKEN RT OVARY (OV1) .1016 .0293 % .0459 WEIGHT TAKEN SPLEEN (SP) .6829 .1971 % .3082 WEIGHT TAKEN LF THYROID/PARA (TT0) .0137 .0040 % .0062 WEIGHT TAKEN RT THYROID/PARA (TT1) .0135 .0039 % .0061 WEIGHT TAKEN UTERUS (UT) .7094 .2047 % .3202 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1364 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92944 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 346.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:16 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1365 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92945 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:06 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.9870 3.6589 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1366 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92945 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:06 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1367 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92946 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 565.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 105; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED, TAN -HYPERPLASIA,-SLIGHT AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 25 X 15 X 12 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT DARK RED, TAN AND FIRM; SAME ON CUT -B-ADENOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) 3126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1368 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92946 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 565.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN LUMEN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SINGLE CLEAR FLUID FILLED CYST, 5 X 3 MODERATELY SEVERE X 3 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1369 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92946 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 565.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 3128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1370 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92947 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 349.0 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT BASOPHILIC,-MINIMAL NECROPSY: NOT OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE 3129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1371 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92947 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 349.0 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT LIGHT GREEN, MUCOID SEMIFLUID -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- 3130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1372 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92947 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 349.0 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1373 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92948 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 96; LIVER (LI) : LIVER (LI) : 10 X 10MM; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: DARK RED, AND TAN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: EOSINOPHILIC CELL,-MINIMAL >SKIN & PELAGE; ROUGH HAIR COAT; MULTIPLE DARK RED AREAS, UP TO 4 MM IN -CONGESTION,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE DIAMETER; COLLECTED ON ROUTINE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >APPEARANCE; THIN; VERIFIED AT SECTIONS NECROPSY: NOT OBSERVED KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MODERATE -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN: -HEMATOPOIESIS, EXTRAMEDULLARY, SINGLE TAN AREA, 6 MM IN DIAMETER INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 23 X 20 X 17 MM (RIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- IS 4 X 3 X 2 MM) SEVERE -PIGMENT,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT -DEGENERATION / NECROSIS, UNILATERAL,- MODERATE 3132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1374 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92948 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT >TISSUE MISSING POSITIVELY IDENTIFIED; SUBMITTED WITH THICKENED ADIPOSE TISSUE (CAP 23) LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -EMBOLISM, ADIPOSE TISSUE,-MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL -CYST / GALACTOCELE,-PRESENT REGION: SINGLE MASS, 30 X 17 X 10 MM, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN AND SOFT; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE MANDIBULAR) -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL 3133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1375 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92948 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -EMBOLISM, PULMONARY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 17 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 10 X 4 MM (CAP 21) LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL, DEEP CERVICAL: EACH >TISSUE MISSING IS UP TO 15 X 7 X 4 MM (CAP 22) ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -THICKENED; SURROUNDING THYMUS: TAN >UNREMARKABLE AND SOFT (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1376 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92948 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1377 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92949 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/04/98 STUDY DAY OF DEATH: 229 STUDY WEEK OF DEATH: 33 TERMINAL BODY WEIGHT: 471.1 GRAMS DATE AND TIME OF NECROPSY: 12/04/98 11:46 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK LIVER (LI) : 3; 80 X 60MM; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HYPERTROPHY, HEPATOCELLULAR, CERVICAL-LEFT; VERIFIED AT NECROPSY: PERIPORTAL,-MINIMAL OBSERVED SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1378 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92949 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/04/98 STUDY DAY OF DEATH: 229 STUDY WEEK OF DEATH: 33 TERMINAL BODY WEIGHT: 471.1 GRAMS DATE AND TIME OF NECROPSY: 12/04/98 11:46 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION, EXTENDING TO BILATERAL SHOULDER REGIONS: SINGLE MASS, 90 X 55 X 45 MM (104.9 GRAMS), TAN, RED, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; SKIN OVERLYING MASS HAS MULTIPLE MOIST, DARK BROWN ABRADED AREAS, UP TO 15 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1379 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92949 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/04/98 STUDY DAY OF DEATH: 229 STUDY WEEK OF DEATH: 33 TERMINAL BODY WEIGHT: 471.1 GRAMS DATE AND TIME OF NECROPSY: 12/04/98 11:46 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1380 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92950 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:41 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.1161 3.7119 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1381 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92950 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:41 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1382 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92951 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:03 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 64; 50 X 50MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >SKIN & PELAGE; SORE/SCAB; AXILLARY EOSINOPHILIC CELL,-MINIMAL REGION-RIGHT; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -FIBROSIS, PERIBILIARY,-SLIGHT WEEK 80; 60 X 45MM; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: OBSERVED PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 51 X 18 X 11 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL 3141 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1383 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92951 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:03 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 X 4 X 3 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT DUODENUM (DU) : -B-LEIOMYOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>THE FIBROADENOMA IS MASS #1 3142 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1384 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92951 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:03 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 68 X 58 X 25 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 65 X 41 X 38 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY RED AND TAN ULCERATED AREA, 50 X 30 X 17 MM; COLLECTED INTACT WITH MASS -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MODERATE CERVIX (CV) : CERVIX (CV) : -MASS(ES); WITHIN LUMEN, EXTENDING -B-POLYP, STROMAL,-NEOPLASM PRESENT INTO BIFURCATION OF UTERINE HORNS: SINGLE POLYPOID MASS, 8 X 3 X 3 MM, RED, GRAY AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) 3143 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1385 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92951 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:03 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; RIGHT: UP TO 15 X 7 X 4 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (LEFT IS UP TO 4 X 3 X 1 MM) (CAP 22) MODERATE LN, INGUINAL (IN) : >TISSUE MISSING >NOTE:>TISSUE IS PART OF MAMMARY TUMOR MASS LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER AND LOWER INCISORS -ANIMAL THIN -------------------------------------------- 3144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1386 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92951 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:03 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1387 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92952 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 300.2 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:09 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 63; 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: OBSERVED PERIPORTAL,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL 3146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1388 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92952 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 300.2 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:09 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 9 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 17 X 15 X 9 MM, DARK RED, -CYST / GALACTOCELE,-PRESENT TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -------------------------------------------- 3147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1389 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92952 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 300.2 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:09 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1390 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92953 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 439.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:23 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR -HYPERPLASIA,-MINIMAL ASPECT: SINGLE BROWN FOCUS, 2 MM IN -ANGIECTASIS,-PRESENT DIAMETER MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1391 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92953 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 439.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:23 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 3150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1392 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92954 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 7:54 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 77; 40 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; RED HAIR COAT; HEAD- PERIPORTAL,-MODERATE ENTIRE; VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; YELLOW HAIR COAT; BASOPHILIC,-SLIGHT VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: BROWN -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 15 X 10 X 7 MM (RIGHT IS >UNREMARKABLE 4 MM IN DIAMETER) -MOTTLED; LEFT: RED, DARK RED AND TAN 3151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1393 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92954 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 7:54 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; RIGHT: 2 X 1 X 1 MM (LEFT IS -B-ADENOMA,-NEOPLASM PRESENT LESS THAN 1 MM) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT RED, TAN ERODED AREAS, UP TO 4 X 2 MM -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1394 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92954 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 7:54 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 50 X 45 X 15 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-NASAL; RED, DRY MATERIAL -------------------------------------------- 3153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1395 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92954 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 7:54 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1396 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92955 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 14:21 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 75; 40 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; DORSAL- -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CERVICAL; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -FIBROSIS, PERIBILIARY,-SLIGHT 83; 20 X 20MM; VERIFIED AT NECROPSY: -INFILTRATE, MACROPHAGE, PIGMENTED,- OBSERVED MINIMAL >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; KIDNEY (KD) : WEEK 84; 25 X 25MM; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MINIMAL >SKIN & PELAGE; YELLOW HAIR COAT; -MINERALIZATION, PELVIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,- >APPEARANCE; THIN; VERIFIED AT MODERATE NECROPSY: OBSERVED -INFLAMMATION, SUPPURATIVE, PELVIC,- >BEHAVIOR; ATAXIC; VERIFIED AT SLIGHT NECROPSY: NOT APPLICABLE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL BONE, FEMUR (FE) : -HYPERPLASIA, CARTILAGE,-MODERATE 3155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1397 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92955 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 14:21 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT VASCULAR AND SOFT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL CERVICAL -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT REGION: SINGLE TAN DRY CRUSTED AREA, 2 -INFLAMMATION, CHRONIC,-MODERATE MM IN DIAMETER (CAP 21) MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 50 X 32 X 15 MM, TAN AND -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT FIRM; SAME ON CUT SURFACE (CAP 22); -HYPERPLASIA, GLANDULAR,-MODERATELY REGIONAL LN (INGUINAL) NOT IDENTIFIED SEVERE -MASS #2; RIGHT INGUINAL REGION: >NOTE:>MASS #3 IS THE CARCINOMA SINGLE MASS, 33 X 24 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; RIGHT INGUINAL REGION, CRANIAL TO MASS 2: SINGLE MASS, 35 X 25 X 20 MM, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT 3156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1398 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92955 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 14:21 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -LARGE, DIFFUSE; BILATERAL: UP TO 6 MM -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- IN DIAMETER SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -------------------------------------------- 3157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1399 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92955 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 14:21 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1400 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92956 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:50 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 82; 40 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; BLUE SKIN; VENTRAL- -RED FOCUS(I)/AREA(S); LEFT LATERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: LOBE: MULTIPLE DARK RED AREAS, UP TO 3 -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NOT APPLICABLE MM IN DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-SLIGHT >SKIN & PELAGE; SORE/SCAB; VENTRAL- SECTION -HEMATOPOIESIS, EXTRAMEDULLARY,- ABDOMINAL-LEFT; VERIFIED AT NECROPSY: MODERATE OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : SPLEEN (SP) : -LARGE; 65 X 15 X 13 MM -HEMATOPOIESIS, EXTRAMEDULLARY, -ADHESION(S); PARIETAL SURFACE OF INCREASED,-SEVERE CAUDAL END TO ADIPOSE TISSUE: MULTIPLE -HEMORRHAGE,-PRESENT GRAY FIBROUS ADHESIONS; COLLECTED -NECROSIS,-MODERATE INTACT -MOTTLED; TAN, RED AND DARK RED PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 3159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1401 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92956 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:50 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATELY SEVERE -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 8 X 5 X 5 -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT MM, RIGHT IS 5 X 4 X 4 MM -HYPERTROPHY, CORTICAL CELL,-SEVERE -MOTTLED; BILATERAL: DARK RED AND -DEGENERATION, CYSTIC / HEMORRHAGIC,- BROWN MODERATELY SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE 3160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1402 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92956 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:50 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, TAN, RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION -M-CARCINOMA,-NEOPLASM PRESENT EXTENDING TO LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 50 X 30 X 25 MM, TAN, -HYPERPLASIA, GLANDULAR,-SLIGHT GRAY, RED, DARK RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (SEE INGUINAL); OVERLYING SKIN IS ULCERATED, 40 X 20 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; BILATERAL: LEFT IS 6 X 5 X 4 -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE MM, RIGHT IS 5 X 4 X 3 MM (CAP 21) LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; PANCREATIC: UP TO 2 X 2 X 1 MM -HEMORRHAGE,-PRESENT (CAP 23) -HYPERPLASIA, LYMPHOPLASMACYTIC,- -DIFFUSELY RED; PANCREATIC: DARK RED MODERATE (CAP 23) -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1403 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92956 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:50 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1404 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92957 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:18 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 63; 100 X 80MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 10 X 6 X 5 MM >UNREMARKABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR -HYPERPLASIA,-SLIGHT ASPECT: SINGLE DARK RED, SLIGHTLY RAISED AREA, 3 X 2 MM STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 4 X 1 MM 3163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1405 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92957 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:18 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ COLON (CO) : COLON (CO) : -MASS(ES); 28 MM DISTAL TO CECUM, -B-LEIOMYOMA,-NEOPLASM PRESENT ATTACHED TO SEROSAL SURFACE: SINGLE MASS, 13 X 10 X 7 MM, TAN, YELLOW, AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE MESENTERIC) SKIN (SK) : SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE RIGHT INGUINAL REGION DUE TO -HEMORRHAGE,-PRESENT MASS ON THE LEFT INGUINAL REGION -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 120 X 95 X 40 MM (237 -B-FIBROADENOMA,-NEOPLASM PRESENT GRAMS), TAN, SOFT, AND LOBULATED; SAME -HYPERPLASIA, GLANDULAR,-SLIGHT ON CUT SURFACE; COLLECTED INTACT; >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); RIGHT INGUINAL REGION: SINGLE TAN CREAMY FLUID FILLED CYST, 20 X 15 X 10 MM; COLLECTED ON ROUTINE SECTION >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGIONAL DUE TO MASS ON THE LEFT INGUINAL REGION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1406 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92957 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:18 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1407 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92958 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 327.2 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 10:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HUNCHED; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -DYSTROPHY, CORNEAL IN EARLY STAGES, >SKIN & PELAGE; ROUGH HAIR COAT; CONSISTS PRIMARILY OF FOCAL VERIFIED AT NECROPSY: NOT APPLICABLE MINERALIZATION,-MINIMAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >EXCRETION; FEW FECES; VERIFIED AT OCCASIONALLY NOTED,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1408 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92958 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 327.2 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 10:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 9 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT LIGHT GREEN AND MUCOID CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 3167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1409 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92958 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 327.2 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 10:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1410 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -MOTTLED; ALL LOBES : RED AND TAN; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE COLLECTED ON ROUTINE SECTIONS -VACUOLATION, HEPATOCELLULAR, >EYES; RED DISCHARGE; EYE-RIGHT; PERIPORTAL,-SLIGHT VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >SKIN & PELAGE; BROWN HAIR COAT; EOSINOPHILIC CELL,-MINIMAL VENTRAL-ABDOMINAL; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -INFILTRATION, FAT, INTERSTITIAL,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT -PIGMENT,-SLIGHT 3169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1411 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, SOFT, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, AND TAN STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 1 X 2 MM -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE -EXUDATE, LUMINAL,-PRESENT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT LIGHT GREEN MUCOID MATERIAL -HYPERPLASIA, MUCINOUS,-MINIMAL 3170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1412 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: MISSING -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -------------------------------------------- 3171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1413 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1414 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92960 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:21 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 72; 120 X 80MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -ANGIECTASIS,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 9 X 6 X 4 -HYPERTROPHY, CORTICAL CELL,-MODERATE MM, RIGHT IS 7 X 6 X 4 MM -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 2 MM IN DIAMETER LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1415 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92960 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:21 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SKIN (SK) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 135 X 75 X 60 MM (291.7 -HYPERPLASIA, GLANDULAR,-MINIMAL GRAMS), RED, DARK RED, TAN, GREY, AND FIRM, SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION, DUE TO MASS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1416 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92960 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:21 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1417 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92961 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 120 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >APPEARANCE; LIMITED USE; LIMB-HIND- -HYPERTROPHY, HEPATOCELLULAR, LEFT; VERIFIED AT NECROPSY: NOT PERIPORTAL,-SLIGHT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT SLIGHT NECROPSY: NOT APPLICABLE -HEMORRHAGE,-PRESENT >SKIN & PELAGE; BLUE SKIN; VENTRAL- KIDNEY (KD) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- NOT APPLICABLE MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 X 5 X 3 MM (RIGHT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATE X 3 X 2 MM) -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-MODERATE -ATROPHY, CORTICAL,-MODERATELY SEVERE 3176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1418 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92961 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 9 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: TWO TAN -ACANTHOSIS / HYPERKERATOSIS,-MODERATE ULCERATED AREAS, ONE IS 10 X 4 MM, THE -ULCERATION,-MODERATELY SEVERE OTHER IS 3 X 2 MM -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -B-ADENOMA,-NEOPLASM PRESENT -FIBROSIS,-SEVERE 3177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1419 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92961 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, EXTENDING INTO LEFT PERIANAL REGION: SINGLE MASS, 140 X 70 X 30 MM, RED, DARK RED, TAN, AND SOFT; CUT SURFACE EXUDES A LARGE AMOUNT OF A REDDISH- BROWN SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT PROXIMAL HORN, WITHIN -B-POLYP, ENDOMETRIAL, STROMAL,- LUMEN: SINGLE DARK RED FLUID FILLED NEOPLASM PRESENT CYST, 5 MM IN DIAMETER -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 3178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1420 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92961 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 10:56 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1421 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92962 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 252.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:17 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0267 .0106 % .0127 WEIGHT TAKEN RT ADRENAL (AD1) .0248 .0098 % .0118 WEIGHT TAKEN BRAIN (BR) 2.1071 .8342 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) .9178 .3633 % .4356 WEIGHT TAKEN RT KIDNEY (KD1) .9077 .3593 % .4308 WEIGHT TAKEN LIVER (LI) 7.6198 3.0165 % 3.6162 WEIGHT TAKEN LUNG (LU) 1.4824 .5869 % .7035 WEIGHT TAKEN LF OVARY (OV0) .0547 .0217 % .0260 WEIGHT TAKEN RT OVARY (OV1) .0614 .0243 % .0291 WEIGHT TAKEN SPLEEN (SP) .4245 .1681 % .2015 WEIGHT TAKEN LF THYROID/PARA (TT0) .0071 .0028 % .0034 WEIGHT TAKEN RT THYROID/PARA (TT1) .0086 .0034 % .0041 WEIGHT TAKEN UTERUS (UT) .4418 .1749 % .2097 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1422 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92962 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 252.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:17 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1423 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -COMPRESSION, VENTRAL,-PRESENT WEEK 68; 60 X 50MM; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK -NECROSIS, COAGULATIVE,-MINIMAL 72; 30 X 30MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK PERIPORTAL,-SLIGHT 83; 15 X 15MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; BLACK SKIN; VENTRAL- MINIMAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: SPLEEN (SP) : NOT OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, >SKIN & PELAGE; SORE/SCAB; VENTRAL- INCREASED,-MODERATELY SEVERE ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -ANGIECTASIS,-MODERATE >SKIN & PELAGE; PALE; ENTIRE BODY; -HYPERPLASIA, CORTICAL,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE 3182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1424 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 33 X 28 X 18 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, FIRM, AND LOBULATED; >NOTE:>MASS #3 IS THE FIBROADENOMA. SAME ON CUT SURFACE; COLLECTED; MASS #2 HAS CONSIDERABLE REGIONAL LN (AXILLARY) NORMAL (CAP 21) SQUAMOUS METAPLASIA -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 18 X 15 MM, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A TAN, CASEOUS MATERIAL; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 73 X 45 X 25 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A LIGHT BROWN, CASEOUS SEMISOLID, OTHERS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED AND TAN ULCERATED AREA, 20 MM IN DIAMETER; COLLECTED INTACT WITH MASS 3183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1425 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATELY SEVERE TO 3 MM IN DIAMETER CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 3184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1426 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1427 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92964 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); VENTRAL-CERVICAL; WEEK 83; -VACUOLATION, HEPATOCELLULAR, 30 X 30MM; VERIFIED AT NECROPSY: PERIPORTAL,-SLIGHT OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 6 MM (RIGHT IS -HYPERTROPHY, CORTICAL CELL,-MINIMAL 6 X 4 X 4 MM) -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE >UNREMARKABLE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #2 IS THE FIBROADENOMA 3186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1428 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92964 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); MID VENTRAL CERVICAL REGION: SINGLE MASS, 35 X 30 X 20 MM, TAN, RED, FIRM AND CYSTIC; SOME AREAS EXUDE A CLEAR FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 32 X 22 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MINIMAL TO 4 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 3187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1429 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92964 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1430 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92965 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 573 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 60; BRAIN (BR) : BRAIN (BR) : 45 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: OBSERVED EOSINOPHILIC CELL,-SLIGHT >APPEARANCE; HUNCHED; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE >EXCRETION; NO FECES; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1431 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92965 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 573 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 X MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GREY, TAN, LOBULATED AND SOFT ILEUM (IL) : >AUTOLYTIC SKIN (SK) : >NOTE:>DUE TO MASS ON LEFT INGUINAL REGION, ROUTINE SECTION TAKEN FROM RIGHT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 58 X 43 X 42 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT GREY, TAN, VASCULAR AND FIRM; SAME ON -HYPERPLASIA, GLANDULAR,-SLIGHT CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -CYST(S); RIGHT INGUINAL REGION: SINGLE WHITE, CREAMY SEMIFLUID FILLED CYST, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION (SEE NECROPSY NOTES) >NOTE:>DUE TO MASS ON LEFT INGUINAL REGION, ROUTINE SECTION TAKEN FROM RIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1432 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92965 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 573 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 3191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1433 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92966 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 15:08 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MODERATE >BEHAVIOR; ATAXIC; VERIFIED AT -FIBROSIS, PERIBILIARY,-SLIGHT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -CYST,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1434 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92966 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 15:08 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1435 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92967 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0362 .0127 % .0201 WEIGHT TAKEN RT ADRENAL (AD1) .0327 .0115 % .0182 WEIGHT TAKEN BRAIN (BR) 1.8011 .6311 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1299 .3959 % .6273 WEIGHT TAKEN RT KIDNEY (KD1) 1.1418 .4001 % .6339 WEIGHT TAKEN LIVER (LI) 8.5559 2.9979 % 4.7504 WEIGHT TAKEN LUNG (LU) 1.4342 .5025 % .7963 WEIGHT TAKEN LF OVARY (OV0) .0683 .0239 % .0379 WEIGHT TAKEN RT OVARY (OV1) .0575 .0201 % .0319 WEIGHT TAKEN SPLEEN (SP) .5138 .1800 % .2853 WEIGHT TAKEN LF THYROID/PARA (TT0) .0095 .0033 % .0053 WEIGHT TAKEN RT THYROID/PARA (TT1) .0090 .0032 % .0050 WEIGHT TAKEN UTERUS (UT) .8051 .2821 % .4470 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1436 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92967 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1437 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92968 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 14:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE,-SLIGHT >EYES; DILATED PUPIL; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -HEMORRHAGE,-MINIMAL NECROPSY: OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -THROMBUS,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES -CONGESTION,-PRESENT 3196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1438 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92968 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 14:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN, LOBULATED AND SOFT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- 3197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1439 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92968 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 14:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1440 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92969 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:08 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.3530 3.5640 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1441 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92969 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:08 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1442 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92970 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:43 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 4 X 4 MM (RIGHT IS 5 -ANGIECTASIS,-SLIGHT X 3 X 3 MM) -PIGMENT,-SLIGHT -MOTTLED; LEFT: DARK BROWN AND TAN OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 5 MM IN DIAMETER, RIGHT IS 10 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1443 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92970 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:43 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, TAN, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, RED, AND SOFT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT -INFLAMMATION, CHRONIC-ACTIVE,- HINDFOOT: SINGLE MOIST TAN ULCERATED MODERATELY SEVERE AREA, 7 MM IN DIAMETER; COLLECTED -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1444 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92970 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:43 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1445 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92971 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 11:07 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 67; 110 X 110MM; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: TAN -NECROSIS, INDIVIDUAL HEPATOCYTE,- OBSERVED MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -VACUOLATION, HEPATOCELLULAR, ABDOMINAL-LEFT; VERIFIED AT NECROPSY: PERIPORTAL,-SLIGHT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >APPEARANCE; THIN; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -NECROSIS, COAGULATIVE,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : KIDNEY (KD) : -CYST(S); LEFT, MEDULLA: SINGLE CLEAR -NEPHROPATHY, CHRONIC PROGRESSIVE,- FLUID FILLED CYST, 3 MM IN DIAMETER MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT 3204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1446 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92971 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 11:07 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 9 X 7 X 7 -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL MM -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 9 X 8 MM, RED, TAN, AND -B-ADENOMA,-NEOPLASM PRESENT SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT 3205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1447 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92971 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 11:07 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 140 X 130 X 50 MM (303.4 -B-FIBROADENOMA,-NEOPLASM PRESENT GRAMS), RED, TAN, GRAY, LOBULATED AND FIRM; CUT SURFACE CONTAINS A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED ABRADED AREA, 50 MM IN DIAMETER; COLLECTED INTACT UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE TO 3 MM IN DIAMETER CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION -ANIMAL THIN -------------------------------------------- 3206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1448 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92971 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 11:07 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1449 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92972 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 272.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 8:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); SHOULDER-RIGHT; WEEK 72; 55 -COMPRESSION, VENTRAL,-PRESENT X 40MM; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: OBSERVED SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 MM IN -ANGIECTASIS,-MODERATE DIAMETER -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 10 X 9 -B-ADENOMA,-NEOPLASM PRESENT MM, RED, DARK RED, TAN, LOBULATED, AND SOFT 3208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1450 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92972 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 272.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 8:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT DORSAL SHOULDER -CYST / GALACTOCELE,-PRESENT REGION: SINGLE MASS, 55 X 40 X 35 MM, -B-FIBROADENOMA,-NEOPLASM PRESENT RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE LIGHT GREEN CREAMY SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 3209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1451 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92972 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 272.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 8:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1452 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92973 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 610.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -MASS(ES); ALONG MARGIN OF LEFT -FOCUS, ALTERED HEPATOCELLULAR, LATERAL LOBE: SINGLE MASS, 7 X 3 X 3 BASOPHILIC,-SLIGHT MM, RED AND TAN; SAME ON CUT SURFACE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL (CAP 22); REGIONAL LN (HEPATIC) NOT -HYPERPLASIA, BILE DUCT,-SLIGHT IDENTIFIED -FIBROSIS, PERIBILIARY,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED -HEMORRHAGE,-PRESENT LUNG (LU) : -N-CARCINOMA,-FROM THYROID THYROID (TY) : -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT 3211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1453 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92973 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 610.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -MASS(ES); LEFT: SINGLE MASS, 10 MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, LOBULATED, AND FIRM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1454 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92973 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 610.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1455 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92974 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:50 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 104; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 9 MM IN -HYPERTROPHY, CORTICAL CELL,-MODERATELY DIAMETER, RIGHT IS 6 MM IN DIAMETER SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- SEVERE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL 3214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1456 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92974 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:50 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 9 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY AND FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PECTORAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 17 X 8 X 6 MM, TAN AND -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM; ON CUT SECTION EXUDES A TAN -HYPERPLASIA, GLANDULAR,-SLIGHT CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY); POSSIBLY CONSISTENT WITH MASS (VENTRAL-CERVICAL-RIGHT) AS NOTED IN CLINICAL OBSERVATIONS URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE -DILATATION,-MINIMAL CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 22) MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1457 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92974 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:50 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 3216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1458 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92975 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 89; BRAIN (BR) : BRAIN (BR) : 70 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 6 X 4 X 3 MM (RIGHT IS 4 -B-ADENOMA, C-CELL,- 2 NEOPLASMS X 2 X 1 MM) PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 6 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND FIRM 3217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1459 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92975 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); EXTENDS FROM LEFT TO RIGHT -B-FIBROADENOMA,-NEOPLASM PRESENT INGUINAL REGIONS: SINGLE MASS, 100 X 75 X 35 MM, RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1460 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92975 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1461 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92976 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK EYE (EY) : 83; 50 X 50MM TWO MASSES APPEAR TO -DEGENERATION, LENTICULAR,-MODERATELY HAVE GROWN TOGETHER; VERIFIED AT SEVERE NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >EYES; RED DISCHARGE; EYE-LEFT; -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT NECROPSY: NOT APPLICABLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- >APPEARANCE; HEAD TILT; LEFT; VERIFIED MINIMAL AT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- PELVIS: MULTIPLE TAN PINPOINT GRANULES MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 3220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1462 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92976 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE RIGHT ADRENAL: SINGLE MASS, 25 X 20 X 17 MM, DARK RED, RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED AS ROUTINE SECTION; REGIONAL LN (RENAL) NORMAL (CAP 23) ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -N-CARCINOMA,-FROM ADRENAL, CORTEX HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT 3221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1463 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92976 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 50 X 40 X 30 MM, RED, TAN, BROWN, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE INGUINAL) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; LEFT: 5 X 4 X 2 MM (RIGHT IS 3 -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT X 2 X 1 MM) (CAP 21) LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -------------------------------------------- 3222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1464 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92976 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1465 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92977 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES, DIFFUSE: RED AND -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL TAN -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -DEGENERATION / NECROSIS, CENTRILOBULAR,-MINIMAL -LEUKOCYTOSIS, SINUSOIDAL,-MODERATE KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -GRANULAR MATERIAL; BILATERAL, WITHIN MODERATE PELVIS: MULTIPLE HARD TAN CALCULI -DILATATION, PELVIC,-MODERATE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HEMORRHAGE,-PRESENT 3224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1466 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92977 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -MASS(ES); LEFT, REPLACES ENTIRE ADRENAL: SINGLE MASS, 26 X 32 X 45 MM, SOFT, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE; REGIONAL LN (RENAL) NOT IDENTIFIED ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT 3225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1467 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92977 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, ADRENAL,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SINGLE DRY, DARK RED AREA, 5 X 7 MM; -ULCERATION,-MODERATELY SEVERE COLLECTED -ACANTHOSIS / HYPERKERATOSIS,- MODERATELY SEVERE -BACTERIA,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR MOIST MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED MATERIAL -------------------------------------------- 3226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1468 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92977 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1469 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92978 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 336.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:29 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0367 .0109 % .0177 WEIGHT TAKEN RT ADRENAL (AD1) .0305 .0091 % .0147 WEIGHT TAKEN BRAIN (BR) 2.0776 .6174 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1629 .3456 % .5597 WEIGHT TAKEN RT KIDNEY (KD1) 1.1663 .3466 % .5614 WEIGHT TAKEN LIVER (LI) 9.9351 2.9525 % 4.7820 WEIGHT TAKEN LUNG (LU) 1.4846 .4412 % .7146 WEIGHT TAKEN LF OVARY (OV0) .0760 .0226 % .0366 WEIGHT TAKEN RT OVARY (OV1) .0671 .0199 % .0323 WEIGHT TAKEN SPLEEN (SP) .5013 .1490 % .2413 WEIGHT TAKEN LF THYROID/PARA (TT0) .0086 .0026 % .0041 WEIGHT TAKEN RT THYROID/PARA (TT1) .0102 .0030 % .0049 WEIGHT TAKEN UTERUS (UT) .5278 .1568 % .2540 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1470 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92978 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 336.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:29 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1471 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92979 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/01/00 STUDY DAY OF DEATH: 622 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 359.1 GRAMS DATE AND TIME OF NECROPSY: 01/01/00 15:19 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 80; 40 X 35MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE SURFACE OF LEFT LATERAL LOBE: SINGLE -VACUOLATION, HEPATOCELLULAR, >DISCHARGE; GENITAL; RED IN COLOR; DARK RED AREA, 6 MM IN DIAMETER; PERIPORTAL,-MODERATE VERIFIED AT NECROPSY: NOT OBSERVED SUNKEN WITH A STELLATE PATTERN; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; COLLECTED ON ROUTINE SECTION EOSINOPHILIC CELL,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 3230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1472 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92979 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/01/00 STUDY DAY OF DEATH: 622 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 359.1 GRAMS DATE AND TIME OF NECROPSY: 01/01/00 15:19 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -ACANTHOSIS / HYPERKERATOSIS,- TAN ERODED AREA, 7 X 3 MM MODERATELY SEVERE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 35 X 20 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT DARK RED, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW, BROWN AND MOIST -------------------------------------------- 3231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1473 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92979 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/01/00 STUDY DAY OF DEATH: 622 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 359.1 GRAMS DATE AND TIME OF NECROPSY: 01/01/00 15:19 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1474 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92980 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:06 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 38; 30 X 25MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; AXILLARY -LARGE; ALL LOBES: MARGINS APPEAR -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT REGION-LEFT; VERIFIED AT NECROPSY: ROUNDED -VACUOLATION, HEPATOCELLULAR, OBSERVED -MOTTLED; ALL LOBES: RED AND TAN PERIPORTAL,-MINIMAL >MASS(ES); LIMB-HIND-RIGHT; WEEK 55; 60 -FOCUS, ALTERED HEPATOCELLULAR, X 55MM; VERIFIED AT NECROPSY: OBSERVED BASOPHILIC,-MINIMAL >APPEARANCE; MALOCCLUSION; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -MITOSIS, HEPATOCELLULAR, INCREASED,- MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 53 X 11 X 7 MM -HEMATOPOIESIS, EXTRAMEDULLARY, -DIFFUSELY LIGHT; LIGHT RED INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN -ANGIECTASIS,-SLIGHT DIAMETER -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL 3233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1475 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92980 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:06 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 6 X 5 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); RIGHT MID ASPECT: SINGLE DARK RED AREA, 3 X 2 MM STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 30 X 20 X 10 MM, TAN, RED -CYST / GALACTOCELE,-PRESENT AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN IS ULCERATED, RED AND TAN, 20 X 12 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION 3234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1476 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92980 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:06 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 70 X 40 X 35 MM, TAN, RED, DARK RED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) VAGINA (VA) : >TISSUE MISSING CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 9 X 7 X 3 MM (FOR -HYPERPLASIA, LYMPHOPLASMACYTIC,- COMPARISON, RIGHT IS UP TO 5 X 3 X 1 MODERATE MM)(CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -------------------------------------------- 3235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1477 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92980 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:06 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 3236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1478 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92981 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 346.0 GRAMS DATE AND TIME OF NECROPSY: 09/03/99 8:11 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; HEAD- LIVER (LI) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT >APPEARANCE; HEAD TILT; LEFT; VERIFIED PANCREAS (PA) : AT NECROPSY: NOT APPLICABLE -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- >EXCRETION; NO FECES; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 6 MM, RED, TAN, -B-ADENOMA,-NEOPLASM PRESENT AND FIRM VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY RED MATERIAL -------------------------------------------- 3237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1479 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92981 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 346.0 GRAMS DATE AND TIME OF NECROPSY: 09/03/99 8:11 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1480 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92982 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:22 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.7810 3.5707 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1481 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92982 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:22 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1482 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92983 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 94; 45 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-CERVICAL; WEEK 96; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VERIFIED AT NECROPSY: NOT OBSERVED -VACUOLATION, HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT PERIPORTAL,-SLIGHT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT SKIN (SK) : SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION 3241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1483 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92983 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 58 X 45 X 25 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, LOBULATED, AND FIRM; -HYPERPLASIA, GLANDULAR,-SLIGHT SOME SECTIONS EXUDE A TAN CREAMY SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS DIFFUSE: COLLECTED ON ROUTINE SECTION >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1484 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92983 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1485 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92984 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 498.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 75; 50 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 75; 50 X 40MM; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- >MASS(ES); AXILLARY REGION-LEFT; WEEK SLIGHT 93; 15 X 10MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, OBSERVED PERIPORTAL,-SLIGHT >MASS(ES); VENTRAL-ABDOMINAL; WEEK 104; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 15 X 15MM; VERIFIED AT NECROPSY: EOSINOPHILIC CELL,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE 3244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1486 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92984 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 498.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 20 X 18 X 8 MM, TAN AND -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION, EXTENDING INTO RIGHT PERIANAL REGION: MULTIPLE MASSES, UP TO 60 X 50 X 25 MM, ALL ARE TAN, RED, DARK RED, AND FIRM; ALL ARE SAME ON CUT SURFACE; PORTIONS COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1487 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92984 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 498.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1488 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92985 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK -COMPRESSION, VENTRAL,-PRESENT 76; 80 X 80MM; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -MOTTLED; ALL LOBES, DIFFUSE: RED AND -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; MISSING; TEETH; VERIFIED TAN -VACUOLATION, HEPATOCELLULAR, AT NECROPSY: OBSERVED PERIPORTAL,-MODERATE >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 82; 20 X 20MM; VERIFIED AT NECROPSY: EOSINOPHILIC CELL,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BASOPHILIC,-MODERATE WEEK 95; 20 X 20MM; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK KIDNEY (KD) : 95; 10 X 10MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- NOT OBSERVED MINIMAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK -MINERALIZATION, PELVIC,-MODERATE 96; 25 X 25MM; VERIFIED AT NECROPSY: SPLEEN (SP) : OBSERVED -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1489 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92985 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, SOFT, RED, -B-ADENOMA,-NEOPLASM PRESENT AND TAN MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 27 X 18 X 15 MM, FIRM, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT LOBULATED, RED, AND TAN; SAME ON CUT -HYPERPLASIA, GLANDULAR,-MODERATE SURFACE; COLLECTED; REGIONAL LN >NOTE:>MASS #'S 1 AND 5 ARE (INGUINAL) NOT IDENTIFIED FIBROADENOMAS. MASS #'S 3 AND 4 -MASS #2; LEFT INGUINAL REGION, CAUDAL ARE CARCINOMAS MASS #2: SEE TO MASS NUMBER ONE: SINGLE MASS, 37 X CLITORAL GLAND FOR DIAGNOSIS 25 X 20 MM, RED, TAN, BROWN, AND SOFT; ON SECTION SOME AREAS CONTAIN A TAN AND DRY SEMISOLID MATERIAL; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1490 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92985 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 22 X 14 X 11 MM, FIRM, RED, TAN, AND LOBULATED; SAME ON CUT SURFACE, COLLECTED; REGIONAL LN INGUINAL (NOT IDENTIFIED) -MASS #4; RIGHT INGUINAL, CAUDAL TO MASS NUMBER THREE: SINGLE MASS, 45 X 30 X 20 MM, FIRM, LOBULATED, RED, DARK RED, AND TAN; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #5; RIGHT AXILLARY REGION: SINGLE MASS, 75 X 75 X 55 MM, FIRM, LOBULATED, RED, DARK RED, TAN, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A 30 MM IN DIAMETER DRY, BROWN CRUSTED AREA; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 23) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-SLIGHT MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT CLEAR MUCOID MATERIAL -HYPERPLASIA, MUCINOUS,-SEVERE 3249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1491 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92985 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE CLITORAL GLAND (CL) : -ECTASIA, DUCTAL,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -HYPERKERATOSIS,-SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING -------------------------------------------- 3250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1492 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92985 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1493 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92986 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 85; 70 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 8 X 5 MM, DARK RED, AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -B-ADENOMA,-NEOPLASM PRESENT 3252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1494 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92986 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: >NOTE:>SMALL ADENOMA NOTED ON REGULAR SINGLE MASS, 100 X 65 X 30 MM (104.7 MAMMARY GLAND SECTION MASSES 1 GRAMS), RED, TAN, LOBULATED, AND AND 2 WERE FIBROADENOMAS VASCULAR; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 23 X 15 X 6 MM, TAN, AND FIRM, SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS SEMIFLUID (CAP 23); REGIONAL LN (INGUINAL) NORMAL (CAP 22) UTERUS (UT) : UTERUS (UT) : -MASS(ES); LEFT HORN, WITHIN LUMEN: -B-POLYP, ENDOMETRIAL, STROMAL,- SINGLE MASS, 35 X 10 X 10 MM, RED, NEOPLASM PRESENT TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 25) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1495 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92986 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1496 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92987 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:46 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0307 .0112 % .0134 WEIGHT TAKEN RT ADRENAL (AD1) .0327 .0120 % .0143 WEIGHT TAKEN BRAIN (BR) 2.2943 .8395 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1962 .4377 % .5214 WEIGHT TAKEN RT KIDNEY (KD1) 1.1435 .4184 % .4984 WEIGHT TAKEN LIVER (LI) 8.9944 3.2910 % 3.9203 WEIGHT TAKEN LUNG (LU) 1.4595 .5340 % .6361 WEIGHT TAKEN LF OVARY (OV0) .0578 .0211 % .0252 WEIGHT TAKEN RT OVARY (OV1) .0622 .0228 % .0271 WEIGHT TAKEN SPLEEN (SP) .6245 .2285 % .2722 WEIGHT TAKEN LF THYROID/PARA (TT0) .0100 .0037 % .0044 WEIGHT TAKEN RT THYROID/PARA (TT1) .0135 .0049 % .0059 WEIGHT TAKEN UTERUS (UT) .5091 .1863 % .2219 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1497 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92987 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:46 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1498 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92988 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); RIGHT MEDIAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 -VACUOLATION, HEPATOCELLULAR, X 2 MM; COLLECTED ON ROUTINE SECTION PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR -HYPERPLASIA,-SLIGHT ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT 3257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1499 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92988 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 3258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1500 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92989 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -INFLAMMATION, ACUTE,-MINIMAL >EXCRETION; NO FECES; VERIFIED AT BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; LIVER (LI) : VENTRAL-ABDOMINAL; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-MODERATE -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1501 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92989 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 5 MM, APPEARS SWOLLEN -HYPERPLASIA,-SLIGHT -CYST,-PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EDEMA,-SLIGHT MULTIPLE BLACK AREAS, UP TO 3 X 2 MM -INFLAMMATION, ACUTE,-SLIGHT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID VENTRAL THORACIC -M-CARCINOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 10 X 8 X 6 MM, -CYST / GALACTOCELE,-PRESENT TAN AND SOFT; SAME ON CUT SURFACE (CAP -HYPERPLASIA, GLANDULAR,-SLIGHT 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; RIGHT HORN: -DILATATION,-MODERATE FILLED WITH A BROWN FLUID -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CERVIX (CV) : -M-SARCOMA, STROMAL,-NEOPLASM PRESENT 3260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1502 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92989 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : -MASS(ES); WITHIN LUMEN, EXTENDING INTO PROXIMAL UTERUS AND VAGINA: SINGLE MASS, 70 X 50 X 35 MM, DARK BROWN, RED, DARK RED, TAN, AND SOFT; SOME AREAS EXUDE A BROWN FLUID; COLLECTED INTACT; REGIONAL LN (LEFT LUMBAR) NORMAL, RIGHT NOT IDENTIFIED (CAP 23) CAUSE OF DEATH (CD) : -NEOPLASM, UT/CV/VA/OV,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, RETICULOENDOTHELIAL,- SLIGHT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -URINARY BLADDER-LARGE LUMEN; 30 X 15 X 15 MM -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION, EXTENDING TO PERIANAL REGION: BROWN AND MOIST -------------------------------------------- 3261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1503 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92989 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1504 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 73; 65 X 55MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 89; 50 X 90MM; VERIFIED AT NECROPSY: EOSINOPHILIC CELL,-MINIMAL OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT 3263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1505 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 5 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT FIRM SALIV GL, MANDIB (SG) : SALIV GL, MANDIB (SG) : -MISSING/NOT IDENTIFIED; NOT -M-SCHWANNOMA,-NEOPLASM PRESENT IDENTIFIED: NOT POSITIVELY IDENTIFIED; -UNILATERALLY EXAMINED,-PRESENT POSSIBLY COLLECTED WITH CERVICAL MASS NOTED MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1506 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: >NOTE:>MASS #1 IS A FIBROADENOMA SEE SINGLE MASS, 65 X 55 X 35 MM, TAN, SALIVARY GLAND FOR DIAGNOSIS OF LOBULATED AND SOFT; ON SECTION, EXUDES MASS #2 A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; EXTENDS LATERALLY ACROSS ENTIRE VENTRAL CERVICAL REGION: SINGLE MASS, 70 X 45 X 36 MM, TAN, RED, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HYPERPLASIA, LYMPHOPLASMACYTIC,- 21) MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1507 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1508 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92991 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 568.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LIVER (LI) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -HYPERTROPHY, HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 3 MM IN CENTRILOBULAR POLYKARYOCYTOSIS DIAMETER; COLLECTED ON ROUTINE OCCASIONALLY NOTED,-MINIMAL SECTIONS -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 3267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1509 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92991 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 568.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, TAN, AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: -CYST / GALACTOCELE,-PRESENT COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-MODERATE -CYST(S); RIGHT INGUINAL REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK BROWN FLUID FILLED CYST, 40 X 25 X 10 MM (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE DRY, RED, AND TAN CRUSTED AREA, MODERATELY SEVERE 4 X 3 MM; COLLECTED -ULCERATION,-MINIMAL -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; UP TO 8 MM IN DIAMETER (CAP >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 24) >NOTE:>SEE PANCREAS FOR DIAGNOSIS OF MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 3268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1510 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92991 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 568.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DARK RED DRY MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1511 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92992 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); DORSAL; WEEK 76; 20 X 20MM; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -COMPRESSION, VENTRAL,-PRESENT 76; 40 X 40MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO >MASS(ES); AXILLARY REGION-LEFT; WEEK NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 78; 40 X 40MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >MASS(ES); VENTRAL-CERVICAL; WEEK 79; SPLEEN (SP) : 10 X 10MM; VERIFIED AT NECROPSY: -PIGMENT, INCREASED,-SLIGHT OBSERVED PANCREAS (PA) : >MASS(ES); VENTRAL-THORACIC; WEEK 62; -ZYMOGEN GRANULES, DECREASED,-MODERATE 30 X 30MM; VERIFIED AT NECROPSY: -ATROPHY, ACINAR,-MINIMAL OBSERVED ADRENAL, CORTEX (AC) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -VACUOLATION, FOCAL,-SLIGHT 81; 10 X 10MM; VERIFIED AT NECROPSY: -ANGIECTASIS,-MODERATE OBSERVED -HYPERTROPHY, CORTICAL CELL,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL-RIGHT; -PIGMENT,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1512 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92992 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MUSCLE, SKELETAL (SM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MODERATE STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT TAN ERODED AREAS, UP TO 3 X 2 MM SKIN (SK) : SKIN (SK) : -MASS(ES); LEFT DORSAL LUMBAR REGION: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO SINGLE MASS, 25 X 20 X 10 MM, TAN AND NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (DORSAL) AS NOTED IN CLINICAL OBSERVATIONS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT LATERAL CERVICAL -M-CARCINOMA,- 3 NEOPLASMS PRESENT REGION: SINGLE MASS, 20 X 12 X 12 MM, -CYST / GALACTOCELE,-PRESENT DARK RED, RED, TAN, BROWN AND SOFT; -HYPERPLASIA, GLANDULAR,-MODERATE CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (SEE MANDIBULAR) 3271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1513 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92992 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN >NOTE:>CARCINOMA FOUND IN MASS #1, PART AND FIRM; COLLECTED ON ROUTINE SECTION OF MASS #2, AND ON THE SLIDE -MASS #2; ALL REGIONS, DIFFUSE: THAT GROSSLY WAS THOUGHT TO MULTIPLE MASSES, UP TO 45 X 45 X 30 CONTAIN LYMPH NODE. MAJORITY OF MM, ALL ARE TAN, RED AND SOFT; ALL MASS #2 IS FIBROADENOMA. THE EXUDE A TAN CREAMY SEMIFLUID; PORTIONS CARCINOMA ON SLIDE #22 HAS COLLECTED; REGIONAL LN (SEE INGUINAL) CONSIDERABLE SQUAMOUS (AXILLARY) NORMAL (CAP 23) METAPLASIA. HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; LEFT: UP TO 7 X 5 X 5 MM; >NOT REQUIRED TO BE EXAMINED FOR ANIMAL APPEARS CYSTIC (CAP 22); RIGHT NOT >NOTE:>SEE MAMMARY, FEMALE FOR IDENTIFIED DIAGNOSIS LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HYPERPLASIA, LYMPHOPLASMACYTIC,- 21) MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 3272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1514 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92992 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA) ------------------------------------------------------------------------------------------------------------------------------------ 3273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1515 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92993 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 271.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0384 .0142 % .0195 WEIGHT TAKEN RT ADRENAL (AD1) .0376 .0139 % .0191 WEIGHT TAKEN BRAIN (BR) 1.9653 .7247 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1048 .4074 % .5622 WEIGHT TAKEN RT KIDNEY (KD1) 1.1773 .4341 % .5990 WEIGHT TAKEN LIVER (LI) 7.6933 2.8368 % 3.9146 WEIGHT TAKEN LUNG (LU) 1.1907 .4390 % .6059 WEIGHT TAKEN LF OVARY (OV0) .0548 .0202 % .0279 WEIGHT TAKEN RT OVARY (OV1) .0542 .0200 % .0276 WEIGHT TAKEN SPLEEN (SP) .5039 .1858 % .2564 WEIGHT TAKEN LF THYROID/PARA (TT0) .0103 .0038 % .0052 WEIGHT TAKEN RT THYROID/PARA (TT1) .0083 .0031 % .0042 WEIGHT TAKEN UTERUS (UT) 1.0577 .3900 % .5382 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -NECROSIS, COAGULATIVE,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1516 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92993 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 271.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1517 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; CONSISTS PRIMARILY OF FOCAL VERIFIED AT NECROPSY: NOT OBSERVED MINERALIZATION,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -INFLAMMATION, ACUTE,-SLIGHT NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >SKIN & PELAGE; YELLOW HAIR COAT; -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -DEPLETION, LYMPHOCYTIC,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1518 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -MASS(ES); ATTACHED TO MID PORTION OF DUODENUM: SINGLE MASS, 33 X 17 X 12 MM, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT -DEPLETION, LYMPHOCYTIC (GENERALLY POSITIVELY IDENTIFIED; POSSIBLE COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- REMNANTS COLLECTED MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, GREY AND FIRM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT STOMACH, GL (ST) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT RECTUM (RE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1519 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ RECTUM (RE) : -MASS(ES); ATTACHED TO SEROSAL SURFACE: SINGLE MASS, 11 X 11 X 9 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE ILIAC) MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT LIGHT GREEN, CREAMY SEMIFLUID MATERIAL HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 13 X 9 X 6 MM (CAP 21) -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -DIFFUSELY RED; DARK RED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT ILIAC: UP TO 7 X 5 X 2 MM -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO (RIGHT ILIAC NOT IDENTIFIED) (CAP 23) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -DIFFUSELY RED; LEFT: DARK RED -MASS(ES); BILATERAL, LUMBAR INVOLVING ENTIRE ORGAN: SINGLE MASS, 40 X 25 X 20 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 25) LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT NOT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO IDENTIFIED) (CAP 24) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1520 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, POPLITEAL (PP) : -DIFFUSELY RED; LEFT: DARK RED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 3279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1521 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV) ------------------------------------------------------------------------------------------------------------------------------------ 3280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1522 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92995 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/98 STUDY DAY OF DEATH: 59 STUDY WEEK OF DEATH: 9 TERMINAL BODY WEIGHT: 264.0 GRAMS DATE AND TIME OF NECROPSY: 06/17/98 10:39 PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; TAIL; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >APPEARANCE; SWOLLEN; TAIL-DISTAL; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -ATROPHY, TUBULAR,-MINIMAL >APPEARANCE; MISSING; TAIL-DISTAL; SPLEEN (SP) : VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, >SKIN & PELAGE; PALE; ENTIRE BODY; INCREASED,-MODERATELY SEVERE VERIFIED AT NECROPSY: NOT APPLICABLE UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT TAIL (TI) : TAIL (TI) : -ABRASION(S)/ULCERATION(S); 120 MM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE FROM BASE: MULTIPLE RED, TAN AND MOIST -ULCERATION,-SLIGHT ABRADED AREAS, UP TO 7 X 6 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; 120 MM FROM BASE: TIP MISSING; REMAINING PORTION COLLECTED -------------------------------------------- 3281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1523 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92995 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/98 STUDY DAY OF DEATH: 59 STUDY WEEK OF DEATH: 9 TERMINAL BODY WEIGHT: 264.0 GRAMS DATE AND TIME OF NECROPSY: 06/17/98 10:39 PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1524 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92996 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:01 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.1652 3.5765 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1525 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92996 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:01 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1526 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92997 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 307.6 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:23 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 74; 30 X 30MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED EOSINOPHILIC CELL,-MINIMAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MOTTLED; BILATERAL: RED AND BROWN -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: -DEPLETION, LYMPHOCYTIC (GENERALLY MULTIPLE DARK RED AREAS, PINPOINT AND COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- UP TO 1 MM IN DIAMETER MINIMAL 3285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1527 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92997 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 307.6 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:23 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 5 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 20 X 20 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT TAN, FIRM AND LOBULATED; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL -------------------------------------------- 3286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1528 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92997 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 307.6 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:23 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1529 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92998 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 316 STUDY WEEK OF DEATH: 46 TERMINAL BODY WEIGHT: 205.8 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DIFFUSELY DARK; DIAPHRAGMATIC SURFACE -HYPERPLASIA, BILE DUCT,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL; VERIFIED OF LEFT LATERAL LOBE: SINGLE BROWN -INFILTRATE, MACROPHAGE, PIGMENTED,- AT NECROPSY: NOT APPLICABLE FOCI 2 MM IN DIAMETER MINIMAL >SKIN & PELAGE; YELLOW HAIR COAT; KIDNEY (KD) : VERIFIED AT NECROPSY: NOT OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT -PIGMENT, TUBULAR,-MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE MARROW, FEMUR (FM) : -CONGESTION,-PRESENT MARROW, STERNUM (SE) : -CONGESTION,-PRESENT SPINAL CORD (SC) : -AXONAL SWELLING,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1530 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92998 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 316 STUDY WEEK OF DEATH: 46 TERMINAL BODY WEIGHT: 205.8 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GREY AND SOFT -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-MODERATE AND FIRM; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -------------------------------------------- 3289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1531 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92998 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 316 STUDY WEEK OF DEATH: 46 TERMINAL BODY WEIGHT: 205.8 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1532 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92999 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/22/00 15:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 59; EYE (EY) : 40 X 40MM; VERIFIED AT NECROPSY: -DYSTROPHY, CORNEAL IN EARLY STAGES, OBSERVED CONSISTS PRIMARILY OF FOCAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED MINERALIZATION,-MINIMAL AT NECROPSY: NOT APPLICABLE BRAIN (BR) : BRAIN (BR) : >APPEARANCE; THIN; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-MODERATELY SEVERE -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT 3291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1533 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92999 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/22/00 15:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 4 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 45 X 35 X 16 MM, TAN, -INFLAMMATION, CHRONIC-ACTIVE,- GREEN, VASCULAR AND SOFT; ON SECTION MODERATELY SEVERE EXUDES A GREEN CREAMY SEMIFLUID; -B-ADENOMA,-NEOPLASM PRESENT COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL) NOTED IN CLINICAL OBSERVATION UTERUS (UT) : -DILATATION,-SLIGHT 3292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1534 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92999 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/22/00 15:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,- -LARGE; BILATERAL: EACH IS 10 X 7 X 2 MODERATELY SEVERE MM; COLLECTED GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- 3293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1535 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C92999 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/22/00 15:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1536 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93000 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:56 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0366 .0137 % .0176 WEIGHT TAKEN RT ADRENAL (AD1) .0430 .0161 % .0207 WEIGHT TAKEN BRAIN (BR) 2.0797 .7772 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1525 .4307 % .5542 WEIGHT TAKEN RT KIDNEY (KD1) 1.1261 .4208 % .5415 WEIGHT TAKEN LIVER (LI) 9.1952 3.4362 % 4.4214 WEIGHT TAKEN LUNG (LU) 1.4706 .5496 % .7071 WEIGHT TAKEN LF OVARY (OV0) .0643 .0240 % .0309 WEIGHT TAKEN RT OVARY (OV1) .0895 .0334 % .0430 WEIGHT TAKEN SPLEEN (SP) .4800 .1794 % .2308 WEIGHT TAKEN LF THYROID/PARA (TT0) .0115 .0043 % .0055 WEIGHT TAKEN RT THYROID/PARA (TT1) .0225 .0084 % .0108 WEIGHT TAKEN UTERUS (UT) .6052 .2262 % .2910 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1537 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93000 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:56 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1538 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93001 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 321.6 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 82; 10 X 10MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BASOPHILIC,-SLIGHT AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT -MINERALIZATION, PELVIC,-SLIGHT NECROPSY: NOT APPLICABLE SPLEEN (SP) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -PIGMENT, INCREASED,-SLIGHT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -HYPERPLASIA, CORTICAL,-SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 3297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1539 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93001 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 321.6 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 17 X 15 X 8 MM, TAN, RED -CYST / GALACTOCELE,-PRESENT AND SOFT; CUT SURFACE EXUDES A TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT CREAMY SEMIFLUID (CAP 21); REGIONAL LN >NOTE:>MASS #1 IS THE FIBROADENOMA (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT PERIANAL REGION: SINGLE MASS, 35 X 32 X 20 MM, DARK RED, TAN, YELLOW AND SOFT; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL 3298 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1540 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93001 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 321.6 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3299 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1541 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93002 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 56; 70 X 55MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 70; 20 X 20MM; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, OBSERVED PERIPORTAL,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED BASOPHILIC,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >SKIN & PELAGE; RED HAIR COAT; HEAD- SPLEEN (SP) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, >RESPIRATION; LABORED; VERIFIED AT INCREASED,-SLIGHT NECROPSY: NOT APPLICABLE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER 3300 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1542 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93002 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; DUE TO LUNG -M-THYMIC CARCINOMA,-NEOPLASM PRESENT MASS; THYMUS NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED LUNG (LU) : LUNG (LU) : -MASS(ES); INVOLVING ENTIRE RIGHT >UNREMARKABLE CAUDAL AND MIDDLE LOBES: SINGLE MASS, >NOTE:>SEE THYMUS FOR DIAGNOSIS OF MASS 48 X 23 X 18 MM, RED, DARK RED, GRAY, TAN, LOBULATED, VASCULAR, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NORMAL (CAP 22); PORTION OF MASS ADHERED TO DIAPHRAGM; ADHESIONS COLLECTED WITH MASS HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 11 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT LOBULATED, AND SOFT 3301 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1543 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93002 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 48 X 30 X 18 MM, RED, -CYST / GALACTOCELE,-PRESENT DARK RED, LOBULATED, AND SOFT; ON -HYPERPLASIA, GLANDULAR,-SLIGHT SECTION EXUDES A BROWN FLUID (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 55 X 40 X 40 MM, RED, DARK RED, BLACK, GRAY, TAN, VASCULAR, LOBULATED, AND SOFT; ON SECTION EXUDES A BROWN FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT GREEN MUCOID SEMIFLUID MATERIAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; LEFT: UP TO 8 X 5 X 3 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (RIGHT IS UP TO 4 X 4 X 3 MM) (CAP 21) MODERATELY SEVERE 3302 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1544 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93002 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, TRACHEOBRON (TB) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY, DARK RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY; POSSIBLY CONSISTENT WITH (RED HAIRCOAT ENTIRE HEAD) NOTED IN CLINICAL OBSERVATIONS GENERAL INFORMATION (XX) : >NOTE:>OCULAR DISCHARGE (LEFT EYE) NOTED IN CLINICAL OBSERVATIONS WAS NOT SEEN AT NECROPSY -------------------------------------------- 3303 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1545 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93002 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3304 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1546 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93003 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 625.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK MULTIPLE DARK RED AREAS, UP TO 4 X 3 -NECROSIS, INDIVIDUAL HEPATOCYTE,- 56; 90 X 90MM; VERIFIED AT NECROPSY: MM, SOME ARE SUNKEN WITH A STELLATE MINIMAL OBSERVED PATTERN; COLLECTED ON ROUTINE SECTIONS -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; SORE/SCAB; AXILLARY PERIPORTAL,-SLIGHT REGION-LEFT; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MINIMAL >SKIN & PELAGE; YELLOW HAIR COAT; -FOCUS, ALTERED HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED BASOPHILIC,-SLIGHT -NECROSIS, COAGULATIVE,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 3 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3305 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1547 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93003 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 625.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -COLLOID, DECREASED,-SEVERE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 105 X 105 X 45 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT TAN, LOBULATED, AND SOFT; SAME ON CUT SURFACE; OVERLYING SKIN HAS A MOIST RED ULCERATED AREA, 35 X 30 MM; COLLECTED INTACT WITH MASS; REPRESENTATIVE PORTIONS COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS-PERINEUM/PERIANAL; DRY YELLOW MATERIAL -------------------------------------------- 3306 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1548 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93003 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 625.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3307 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1549 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93004 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 322.9 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; SQUINTED; PERI-ORBITAL-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; ATAXIC; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- IS 4 X 3 X 3 MM) MODERATELY SEVERE OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL 3308 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1550 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93004 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 322.9 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 9 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, LOBULATED AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HEMORRHAGE,-PRESENT 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 3309 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1551 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93004 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 322.9 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3310 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1552 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93005 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:01 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 51; 40 X 45MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: OBSERVED EOSINOPHILIC CELL,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; ATAXIC; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHORETICULAR,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3311 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1553 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93005 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:01 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -HYPERPLASIA, LYMPHORETICULAR,-SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 9 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -EDEMA,-PRESENT -INFLAMMATION, SUBACUTE,-MODERATE SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 55 X 50 X 30 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, FIRM AND LOBULATED; -HYPERPLASIA, GLANDULAR,-SLIGHT SOME SECTIONS EXUDE A TAN, CREAMY -B-ADENOMA,-NEOPLASM PRESENT SEMISOLID; COLLECTED; REGIONAL LN >NOTE:>SEE ALSO SKIN, AS MASS CONTAINS (AXILLARY) NORMAL (CAP 21) TWO DISTINCT COMPONENTS. MASS 1 -CYST(S); ALL REGIONS, DIFFUSE: IS THE FIBROADENOMA MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3312 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1554 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93005 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:01 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : -HYPERPLASIA, RETICULOENDOTHELIAL,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 3313 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1555 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93005 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:01 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3314 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1556 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93006 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:39 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.9154 3.5657 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3315 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1557 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93006 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:39 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3316 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1558 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93007 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN -HYPERPLASIA,-MINIMAL SKIN (SK) : SKIN (SK) : -THICKENED; ALL REGIONS, DIFFUSE: TAN >NOT REQUIRED TO BE EXAMINED FOR ANIMAL AND FIRM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CERVIX (CV) : CERVIX (CV) : -LARGE; 11 X 10 X 5 MM; FIRM -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3317 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1559 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93007 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3318 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1560 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93008 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:38 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.8828 3.7198 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3319 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1561 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93008 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:38 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3320 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1562 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93009 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 15:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; EYE (EY) : EYE (EY) : WEEK 79; 10 X 10MM; VERIFIED AT -ROUGHENED CORNEA; LEFT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE NECROPSY: OBSERVED -ULCERATION, CORNEAL,-MODERATE >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 91; 25 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; SQUINTED; PERI-ORBITAL-RIGHT; -VACUOLATION, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT APPLICABLE PERIPORTAL,-MINIMAL >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >EYES; SCAB; EYE-LEFT; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, TUBULAR,-MINIMAL NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT 3321 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1563 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93009 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 15:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 7 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 25 X 19 X 12 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 9 X 5 X 3 -HYPERPLASIA, LYMPHOPLASMACYTIC,- MM; COLLECTED MODERATE AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT 3322 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1564 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93009 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 15:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CALVARIUM/SKULL (CA) : CALVARIUM/SKULL (CA) : -MASS(ES); LEFT SIDE OF HEAD, NEAR >NOT REQUIRED TO BE EXAMINED FOR ANIMAL BASE OF EAR: SINGLE MASS, 25 X 24 X 18 >NOTE:>SEE AUDITORY SEBACEOUS GLAND FOR MM, RED, TAN AND FIRM; SAME ON CUT DIAGNOSIS SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -------------------------------------------- 3323 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1565 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93009 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 15:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3324 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1566 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93010 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 15:04 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; BROWN HAIR COAT; LIVER (LI) : LIVER (LI) : PERINEAL AREA; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: BROWN AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; HEAD TILT; RIGHT; VERIFIED PERIPORTAL,-MODERATE AT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT BASOPHILIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -FIBROSIS, PERIBILIARY,-MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN -MINERALIZATION, PELVIC,-MODERATE PELVIS: MULTIPLE TAN AND FIRM -HYPERPLASIA, TRANSITIONAL CELL,- GRANULES, UP TO 1 MM IN DIAMETER MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 3325 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1567 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93010 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 15:04 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 15 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -DILATATION,-MODERATE CERVIX (CV) : -B-POLYP, STROMAL,-NEOPLASM PRESENT VAGINA (VA) : VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, -HYPERPLASIA, MUCINOUS,-SLIGHT 10 X 7 X 7 MM, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -LYMPHANGIECTASIS,-PRESENT FILLED CYST, 4 X 3 X 3 MM (CAP 21) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE 3326 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1568 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93010 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 15:04 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST MATERIAL -------------------------------------------- 3327 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1569 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93010 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 15:04 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3328 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1570 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93011 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 626.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-SLIGHT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, MACROPHAGE, ALVEOLAR,- SURFACE OF RIGHT CAUDAL LOBE: SINGLE MINIMAL TAN AREA, 2 MM IN DIAMETER; COLLECTED INTACT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, -HYPERPLASIA,-MODERATE TAN, GRAY AND FIRM -ANGIECTASIS,-PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN WALL OF LEFT HORN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CYSTS, SOME ARE CLEAR FLUID MODERATE FILLED AND SOME ARE RED FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER 3329 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1571 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93011 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 626.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : VAGINA (VA) : -MASS(ES); ATTACHED TO SEROSAL -HYPERPLASIA, MUCINOUS,-SLIGHT SURFACE: SINGLE MASS, 12 X 6 X 2 MM, TAN, SOFT AND VAGINA LIKE APPEARANCE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3330 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1572 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93011 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 626.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3331 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1573 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 37; 115 X 95MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >SKIN & PELAGE; BLACK SKIN; VENTRAL- BASOPHILIC,-MINIMAL THORACIC-LEFT; VERIFIED AT NECROPSY: KIDNEY (KD) : NOT OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; SORE/SCAB; VENTRAL- MINIMAL THORACIC-LEFT; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL OBSERVED SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MODERATELY SEVERE -DEGENERATION / NECROSIS, UNILATERAL,- SEVERE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3332 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1574 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -THICKENED WALL; MUCOSA >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL 3333 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1575 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION, EXTENDING TO LEFT LATERAL THORACIC REGION: SINGLE MASS, 113 X 85 X 61 MM (214.0 GRAMS), RED, DARK RED, GRAY, GREEN, BLACK, TAN, LOBULATED, VASCULAR, CYSTIC, AND FIRM; SOME SECTIONS EXUDE A GREEN MUCOID SEMIFLUID MATERIAL; PORTIONS COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY, BLACK CRUSTED AREA, 65 X 40 MM; COLLECTED WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS UP TO 10 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 8 X 6 MM, RIGHT IS UP TO 7 X 5 X 4 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3334 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1576 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3335 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1577 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93013 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 262.1 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:07 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; ATAXIC; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE -INFILTRATE, FAT, INTERSTITIAL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 3336 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1578 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93013 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 262.1 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:07 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 6 X 6 MM, TAN AND SOFT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND SOFT; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL -------------------------------------------- 3337 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1579 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93013 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 262.1 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:07 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3338 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1580 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93014 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 253.3 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: JILL PAUS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 72; 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : 73; 10 X 10MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >EYES; RED DISCHARGE; EYES; VERIFIED AT BASOPHILIC,-MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE -PIGMENT, TUBULAR,-MINIMAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED SPLEEN (SP) : AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE >EXCRETION; NO FECES; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -ZYMOGEN GRANULES, DECREASED,- >APPEARANCE; HUNCHED; VERIFIED AT MODERATELY SEVERE NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT IS 4 -DEGENERATION, CYSTIC / HEMORRHAGIC,- X 2 X 2 MM) MODERATELY SEVERE -DIFFUSELY RED; LEFT: DARK RED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 3339 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1581 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93014 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 253.3 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: JILL PAUS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 7 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -RED FOCUS(I)/AREA(S); MUCOSA: -ULCERATION,-SLIGHT MULTIPLE AREAS, PINPOINT AND UP TO 2 X -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY FLUID FILLED -INFLAMMATION, CHRONIC,-MODERATE CYSTS, UP TO 12 MM IN DIAMETER (CAP 21); POSSIBLY CONSISTENT WITH MASSES AS NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT, WITHIN HORN: SINGLE -CYST,-MODERATE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -------------------------------------------- 3340 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1582 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93014 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 253.3 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: JILL PAUS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3341 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1583 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93015 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 11:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; BRAIN (BR) : BRAIN (BR) : 40 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; RED DISCHARGE; EYES; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: OBSERVED EOSINOPHILIC CELL,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN -ANGIECTASIS,-MODERATELY SEVERE DIAMETER -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-SLIGHT 3342 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1584 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93015 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 11:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID ABDOMINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 45 X 30 X 30 MM, RED, TAN -B-FIBROADENOMA,-NEOPLASM PRESENT AND LOBULATED; SOME SECTIONS EXUDE A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF LEFT HORN: -B-POLYP, ENDOMETRIAL, STROMAL,- SINGLE MASS, 25 X 4 X 3 MM, RED, TAN NEOPLASM PRESENT AND SOFT; COLLECTED INTACT WITH -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT UTERUS; REGIONAL LN (LUMBAR) NORMAL (CAP 21) 3343 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1585 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93015 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 11:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : -HYPERPLASIA, STROMAL,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST YELLOW MATERIAL -------------------------------------------- 3344 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1586 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93015 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 11:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3345 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1587 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93016 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 571 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/11/99 7:46 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -HEMORRHAGE,-MINIMAL AT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT APPLICABLE PERIPORTAL,-MINIMAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -INFILTRATE, MACROPHAGE, PIGMENTED,- AT NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT OBSERVED -LARGE PELVIS(ES); RIGHT -DILATATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : PANCREAS (PA) : -MASS(ES); ATTACHED TO CRANIAL PORTION -M-CARCINOMA, ISLET CELL,-NEOPLASM OF DUODENUM: SINGLE MASS, 17 X 14 X 10 PRESENT MM, RED, DARK RED, TAN AND SOFT; ON SECTION EXUDES A RED, SEMIFLUID MATERIAL (CAP 22); REGIONAL LN (SEE PANCREATIC) ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT IS 5 -ANGIECTASIS,-SLIGHT X 4 X 3 MM) -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 3346 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1588 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93016 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 571 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/11/99 7:46 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : LUNG (LU) : -UNCOLLAPSED; ALL LOBES >UNREMARKABLE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, GREY AND SOFT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 12 X -HYPERPLASIA, LYMPHOPLASMACYTIC,- 6 X 3 MM (CAP 21) MODERATELY SEVERE LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 23) -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 3347 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1589 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93016 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 571 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/11/99 7:46 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; UPPER RIGHT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER LEFT INCISOR: MISSING -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3348 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1590 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93017 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 464.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:58 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 33; 60 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -NECROSIS, INDIVIDUAL HEPATOCYTE,- OBSERVED MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE RED FLUID >UNREMARKABLE FILLED CYST, 1 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3349 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1591 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93017 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 464.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:58 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : >AUTOLYTIC HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 6 X 4 X 3 MM (RIGHT IS 4 -B-ADENOMA, C-CELL,-NEOPLASM PRESENT X 3 X 2 MM) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT FIRM SKIN (SK) : >NOTE:>SKIN AND MAMMARY SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO LEFT INGUINAL MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 72 X 65 X 39 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT RED, LOBULATED AND FIRM; SAME ON CUT -HYPERPLASIA, GLANDULAR,-MINIMAL SURFACE; OVERLYING SKIN HAS A RED, TAN >NOTE:>MASS 1 IS THE CARCINOMA AND MOIST ULCERATED AREA, 37 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT 3350 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1592 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93017 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 464.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:58 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT >NOTE:>CARCINOMA GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3351 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1593 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93018 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:57 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0335 .0121 % .0163 WEIGHT TAKEN RT ADRENAL (AD1) .0363 .0132 % .0177 WEIGHT TAKEN BRAIN (BR) 2.0491 .7427 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1939 .4327 % .5826 WEIGHT TAKEN RT KIDNEY (KD1) 1.1675 .4232 % .5698 WEIGHT TAKEN LIVER (LI) 9.0596 3.2837 % 4.4213 WEIGHT TAKEN LUNG (LU) 1.4142 .5126 % .6902 WEIGHT TAKEN LF OVARY (OV0) .0709 .0257 % .0346 WEIGHT TAKEN RT OVARY (OV1) .0673 .0244 % .0328 WEIGHT TAKEN SPLEEN (SP) .4924 .1785 % .2403 WEIGHT TAKEN LF THYROID/PARA (TT0) .0056 .0020 % .0027 WEIGHT TAKEN RT THYROID/PARA (TT1) .0105 .0038 % .0051 WEIGHT TAKEN UTERUS (UT) .5391 .1954 % .2631 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3352 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1594 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93018 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:57 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3353 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1595 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93019 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/22/99 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 292.6 GRAMS DATE AND TIME OF NECROPSY: 02/22/99 13:47 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: NOT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >SKIN & PELAGE; YELLOW HAIR COAT; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS >APPEARANCE; HUNCHED; VERIFIED AT OCCASIONALLY NOTED,-SLIGHT NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >EXCRETION; NO FECES; VERIFIED AT PERIPORTAL,-MODERATE NECROPSY: NOT APPLICABLE KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 3354 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1596 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93019 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/22/99 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 292.6 GRAMS DATE AND TIME OF NECROPSY: 02/22/99 13:47 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -EDEMA,-PRESENT -INFLAMMATION, SUBACUTE,-MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -DISCHARGE-NASAL; BILATERAL: DARK RED AND DRY MATERIAL -------------------------------------------- 3355 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1597 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93019 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/22/99 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 292.6 GRAMS DATE AND TIME OF NECROPSY: 02/22/99 13:47 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3356 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1598 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93020 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/24/99 STUDY DAY OF DEATH: 461 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/24/99 9:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -CONGESTION,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-PRESENT MULTIPLE RED FOCI, UP TO 2 MM IN DIAMETER 3357 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1599 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93020 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/24/99 STUDY DAY OF DEATH: 461 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/24/99 9:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, GRAY, -B-ADENOMA,-NEOPLASM PRESENT TAN, LOBULATED AND SOFT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC MAMMARY, FEMALE (MF) : >SECTION EXAMINED; TISSUE NOT PRESENT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL, >UNREMARKABLE NEAR DISTAL ENDS: DARK GREEN, MUCOID SEMIFLUID CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 20) -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -------------------------------------------- 3358 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1600 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93020 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/24/99 STUDY DAY OF DEATH: 461 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/24/99 9:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3359 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1601 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93021 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 291.5 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -HYPERPLASIA, BILE DUCT,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >SKIN & PELAGE; YELLOW HAIR COAT; PERIPORTAL,-MINIMAL VERIFIED AT NECROPSY: NOT OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3360 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1602 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93021 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 291.5 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 6 MM; -B-ADENOMA,-NEOPLASM PRESENT DARK RED, TAN, GREY, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; -CYST / GALACTOCELE,-PRESENT COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-MODERATE -CYST(S); LEFT PERIANAL REGION: SINGLE CYST, 10 MM IN DIAMETER; FILLED WITH A TAN CREAMY SEMIFLUID (CAP 20) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3361 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1603 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93021 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 291.5 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3362 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1604 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93022 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 87; 60 X 50MM; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR, NECROPSY: OBSERVED MULTIPLE DARK RED FOCI, SOME SUNKEN PERIPORTAL,-SLIGHT WITH A STELLATE PATTERN, UP TO 5 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-MINIMAL SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL IS 4 MM IN DIAMETER) -HYPERTROPHY, CORTICAL CELL,-MODERATE -DARK FOCUS(I)/AREA(S); LEFT: SINGLE -DEGENERATION, CYSTIC / HEMORRHAGIC,- DARK BROWN AREA, 3 MM IN DIAMETER MODERATE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SINGLE DARK RED AREA, 2 X 1 MM 3363 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1605 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93022 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 60 X 50 X 30 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT DARK RED, TAN AND FIRM; SAME ON CUT -HYPERPLASIA, GLANDULAR,-SLIGHT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 30 X 15 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN, NEAR >UNREMARKABLE BIFURCATION: SINGLE BROWN FLUID FILLED CYST, 5 MM IN DIAMETER; CYST RUPTURED AT NECROPSY CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3364 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1606 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93022 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3365 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1607 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93023 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 237.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0484 .0204 % .0235 WEIGHT TAKEN RT ADRENAL (AD1) .0402 .0169 % .0195 WEIGHT TAKEN BRAIN (BR) 2.0570 .8672 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1140 .4696 % .5416 WEIGHT TAKEN RT KIDNEY (KD1) 1.0549 .4447 % .5128 WEIGHT TAKEN LIVER (LI) 7.8631 3.3150 % 3.8226 WEIGHT TAKEN LUNG (LU) 1.1581 .4882 % .5630 WEIGHT TAKEN LF OVARY (OV0) .0665 .0280 % .0323 WEIGHT TAKEN RT OVARY (OV1) .0589 .0248 % .0286 WEIGHT TAKEN SPLEEN (SP) .4895 .2064 % .2380 WEIGHT TAKEN LF THYROID/PARA (TT0) .0077 .0032 % .0037 WEIGHT TAKEN RT THYROID/PARA (TT1) .0074 .0031 % .0036 WEIGHT TAKEN UTERUS (UT) .5715 .2409 % .2778 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3366 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1608 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93023 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 237.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3367 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1609 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93024 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 11:39 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 61; 60 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: OBSERVED EOSINOPHILIC CELL,-MINIMAL >RESPIRATION; IRREGULAR; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; HUNCHED; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3368 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1610 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93024 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 11:39 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 10 X 4 MM, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL ABDOMINAL -CYST / GALACTOCELE,-PRESENT REGION: SINGLE MASS, 65 X 40 X 30 MM, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN, SOFT, VASCULAR, AND LOBULATED; -HYPERPLASIA, GLANDULAR,-SLIGHT SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL -ANIMAL THIN -------------------------------------------- 3369 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1611 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93024 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 11:39 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3370 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1612 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93025 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/23/00 15:03 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT -CYST,-PRESENT 3371 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1613 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93025 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/23/00 15:03 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-MODERATE AND FIRM URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CERVIX (CV) : CERVIX (CV) : -LARGE; 8 MM IN DIAMETER -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: RED, MOIST MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL -------------------------------------------- 3372 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1614 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93025 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/23/00 15:03 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3373 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1615 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93026 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 13:29 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 92; 40 X 30MM; VERIFIED AT NECROPSY: -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >SKIN & PELAGE; BLUE SKIN; VENTRAL- EOSINOPHILIC CELL,-MINIMAL THORACIC-LEFT; VERIFIED AT NECROPSY: -NECROSIS, COAGULATIVE,-MODERATE NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,- >EYES; PALE; EYES; VERIFIED AT MODERATE NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: OBSERVED PERIPORTAL,-MODERATE >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : KIDNEY (KD) : THORACIC-LEFT; VERIFIED AT NECROPSY: -GRANULAR MATERIAL; RIGHT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED PELVIS: MULTIPLE TAN PINPOINT GRANULES MODERATE -DIFFUSELY LIGHT; BILATERAL: TAN -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 48 X 12 X 6 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- SLIGHT -INFLAMMATION, VASCULAR,-MODERATELY SEVERE 3374 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1616 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93026 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 13:29 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -ANGIECTASIS,-MODERATELY SEVERE IS 4 MM IN DIAMETER) -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 4 X 4 X 3 MM, RED, GREY, -B-ADENOMA,-NEOPLASM PRESENT CYSTIC AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,- MODERATE -INFLAMMATION, VASCULAR,-MODERATE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3375 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1617 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93026 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 13:29 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 21 X 19 X 11 MM, RED, DARK RED, GREY, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY, RED AND TAN CRUSTED AREA, 15 X 11 MM; COLLECTED WITH MASS; POSSIBLY CONSISTENT WITH SCAB (VENTRAL-THORACIC-LEFT) AS NOTED IN CLINICAL OBSERVATIONS CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 5 X 4 X 3 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (RIGHT IS UP TO 4 X 3 X 2 MM) (CAP 21) MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER AND UPPER INCISORS -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -ANIMAL THIN -------------------------------------------- 3376 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1618 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93026 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 13:29 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3377 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1619 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93027 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:48 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >SKIN & PELAGE; ROUGH HAIR COAT; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: OBSERVED OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- PELVIS: MULTIPLE TAN AND FIRM MINIMAL GRANULES, UP TO 1 MM IN DIAMETER -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT 3378 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1620 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93027 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:48 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 20 X 15 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN AND CREAMY SEMIFLUID -HYPERPLASIA, GLANDULAR,-MODERATE FILLED CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -HYPERPLASIA, MUCINOUS,-SLIGHT GREEN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -------------------------------------------- 3379 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1621 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93027 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:48 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3380 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1622 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93028 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 338.8 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 14:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 88; 10 X10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 91; 15 X 15MM; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >EYES; RED DISCHARGE; EYES; VERIFIED AT EOSINOPHILIC CELL,-MODERATE NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-MODERATE >BEHAVIOR; HYPOACTIVE; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 3381 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1623 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93028 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 338.8 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 14:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 20 MM IN DIAMETER, TAN, -CYST / GALACTOCELE,-PRESENT RED, AND FIRM; SAME ON CUT SURFACE; -B-FIBROADENOMA,-NEOPLASM PRESENT (CAP 21); REGIONAL LN (INGUINAL) NOT -HYPERPLASIA, GLANDULAR,-MODERATE IDENTIFIED >NOTE:>MASS #1 IS THE FIBROADENOMA -MASS #2; RIGHT INGUINAL REGION CRANIAL TO MASS 1: SINGLE MASS, 15 X 12 X 10 MM, TAN, RED AND FIRM; SAME ON CUT SURFACE; (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3382 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1624 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93028 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 338.8 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 14:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3383 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1625 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93029 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:25 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 33; BRAIN (BR) : BRAIN (BR) : 40 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL >SKIN & PELAGE; ROUGH HAIR COAT; SPLEEN (SP) : VERIFIED AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT >EXCRETION; NO FECES; VERIFIED AT -FIBROSIS, CAPSULAR,-PRESENT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >BEHAVIOR; HYPOACTIVE; SEVERE; VERIFIED -DIFFUSELY RED; BILATERAL: DARK RED -ANGIECTASIS,-SLIGHT AT NECROPSY: NOT APPLICABLE OVARY (OV) : OVARY (OV) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT AT NECROPSY: NOT APPLICABLE FILLED CYST, 5 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 5 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -DARK FOCUS(I)/AREA(S); RIGHT AND LEFT ANTERIOR ASPECT: SINGLE DARK BROWN FOCUS AT EACH LOCATION, LEFT IS 1 MM IN DIAMETER, RIGHT IS 3 MM IN DIAMETER 3384 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1626 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93029 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:25 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 35 X 30 X 15 MM, TAN, -CYST / GALACTOCELE,-PRESENT DARK BROWN, RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -DIFFUSELY RED; BILATERAL >UNREMARKABLE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3385 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1627 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93029 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:25 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3386 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1628 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93030 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- -MASS(ES); LEFT, WITHIN PELVIS: SINGLE SLIGHT MASS, TAN AND FIRM; 15 X 13 X 6 MM; -MINERALIZATION, PELVIC,-MODERATE SAME ON CUT SURFACE; COLLECTED INTACT; -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT REGIONAL LN (RENAL) NOT IDENTIFIED -B-LIPOMA,-NEOPLASM PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; RIGHT: 5 X 3 X 3 MM (LEFT IS 6 -ANGIECTASIS,-MODERATE MM IN DIAMETER) -PIGMENT,-SLIGHT 3387 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1629 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93030 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 5 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, GRAY, AND LOBULATED STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN, SLIGHTLY ERODED AREAS, UP TO 2 X -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1 MM CECUM (CE) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID PERINEUM REGION: SINGLE -CYST / GALACTOCELE,-PRESENT MASS, 20 X 15 X 10 MM, RED, TAN AND -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED; -HYPERPLASIA, GLANDULAR,-SLIGHT REGIONAL LN (LUMBAR) NORMAL (CAP 21) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 3388 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1630 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93030 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE HINDFOOT: SINGLE TAN AND MOIST ABRADED -ULCERATION,-MINIMAL AREA, 3 MM IN DIAMETER; COLLECTED -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3389 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1631 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93030 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3390 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1632 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93031 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 183.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:46 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.4393 4.0511 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3391 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1633 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93031 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 183.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:46 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3392 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1634 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93032 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 284.3 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 7:57 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; ATAXIC; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION,-MINIMAL NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; HUNCHED; VERIFIED AT PERIPORTAL,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; FEW FECES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL 3393 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1635 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93032 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 284.3 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 7:57 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 7 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, GRAY, SOFT, AND LOBULATED MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: TWO >UNREMARKABLE CLEAR FLUID FILLED CYSTS, EACH IS 2 MM IN DIAMETER VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3394 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1636 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93032 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 284.3 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 7:57 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3395 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1637 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 45; 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE >DISCHARGE; CLEAR ORAL; MODERATE; -MINERALIZATION, TUBULAR,-MINIMAL VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT >APPEARANCE; THIN; VERIFIED AT SPLEEN (SP) : NECROPSY: OBSERVED -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT 3396 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1638 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFLAMMATION, GRANULOMATOUS,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 10 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT BROWN, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE DARK BROWN AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 18 X 12 X 5 MM, TAN AND -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 15 X 10 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, PINPOINT AND UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT (CAP 22) 3397 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1639 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOW AND MOIST -GI TRACT-LARGE LUMEN; ESOPHAGUS, DIFFUSE: UP TO 10 MM IN DIAMETER -DISCHARGE-ORAL; PINK FLUID -------------------------------------------- 3398 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1640 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3399 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1641 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93034 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/15/99 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK EYE (EY) : EYE (EY) : 72; 15 X 15MM; VERIFIED AT NECROPSY: >NOTE:>TRIMMING COMMENT: ONE EYE -UNILATERALLY EXAMINED,-PRESENT NOT OBSERVED MISSING AT TIME OF TRIMMING >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 73; 80 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 73; 10 X 10MM; VERIFIED AT NECROPSY: -NECROSIS, COAGULATIVE,-MODERATE OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK -HYPERTROPHY, HEPATOCELLULAR, 73; VERIFIED AT NECROPSY: NOT OBSERVED PERIPORTAL,-MODERATELY SEVERE >SKIN & PELAGE; SORE/SCAB; VENTRAL- KIDNEY (KD) : ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT OBSERVED SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 8 X 6 X 5 -ANGIECTASIS,-MODERATE MM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3400 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1642 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93034 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/15/99 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -METAPLASIA, OSSEOUS / CARTILAGINOUS,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 12 X 12 X 9 MM, RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND FIRM; ON SECTION, EXUDES A TAN >NOTE:>THE FIBROADENOMA IS MASS #2 CREAMY SEMIFLUID (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 95 X 69 X 34 MM, RED, TAN, DARK RED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A DARK RED MOIST ULCERATED AREA, 30 X 20 MM, COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3401 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1643 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93034 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/15/99 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3402 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1644 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93035 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:36 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0344 .0124 % .0175 WEIGHT TAKEN RT ADRENAL (AD1) .0292 .0106 % .0148 WEIGHT TAKEN BRAIN (BR) 1.9688 .7118 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2399 .4483 % .6298 WEIGHT TAKEN RT KIDNEY (KD1) 1.2468 .4508 % .6333 WEIGHT TAKEN LIVER (LI) 8.2187 2.9713 % 4.1745 WEIGHT TAKEN LUNG (LU) 1.2172 .4401 % .6182 WEIGHT TAKEN LF OVARY (OV0) .0804 .0291 % .0408 WEIGHT TAKEN RT OVARY (OV1) .0836 .0302 % .0425 WEIGHT TAKEN SPLEEN (SP) .5892 .2130 % .2993 WEIGHT TAKEN LF THYROID/PARA (TT0) .0051 .0018 % .0026 WEIGHT TAKEN RT THYROID/PARA (TT1) .0094 .0034 % .0048 WEIGHT TAKEN UTERUS (UT) 1.4107 .5100 % .7165 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3403 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1645 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93035 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:36 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3404 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1646 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93036 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 592 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/02/99 15:05 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; OBES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -COMPRESSION, VENTRAL,-PRESENT 83; 20 X 20MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >APPEARANCE; TREMORS; ENTIRE BODY; EOSINOPHILIC CELL,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >RESPIRATION; AUDIBLE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >RESPIRATION; IRREGULAR; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >BEHAVIOR; RECUMBENT; DORSAL; VERIFIED SPLEEN (SP) : AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT >APPEARANCE; CONVULSIONS; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY AND SOFT 3405 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1647 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93036 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 592 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/02/99 15:05 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 35 X 28 X 18 MM, RED, -CYST / GALACTOCELE,-PRESENT TAN, FIRM AND LOBULATED; SAME ON CUT -HYPERPLASIA, GLANDULAR,-MODERATELY SURFACE; COLLECTED; REGIONAL LN SEVERE (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -HYPERPLASIA, MUCINOUS,-MODERATELY LIGHT GREEN SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; -HYPERPLASIA, LYMPHOPLASMACYTIC,- COLLECTED INTACT WITH MANDIBULAR MODERATE SALIVARY GLANDS -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 3406 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1648 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93036 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 592 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/02/99 15:05 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3407 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1649 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93037 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 370.4 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 8:05 PROSECTOR: LYNN HORNER RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 64; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >EXCRETION; NO FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-SLIGHT >APPEARANCE; HEAD TILT; LEFT; VERIFIED KIDNEY (KD) : AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >BEHAVIOR; CIRCLING; LEFT; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3408 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1650 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93037 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 370.4 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 8:05 PROSECTOR: LYNN HORNER RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 16 X 12 X 7 MM, RED, DARK RED, GRAY, LOBULATED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION; LARGEST CYSTS ARE POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS (CAP 20) VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT LOWER INCISOR -DISCHARGE-OCULAR; BILATERAL: DARK RED DARK AND DRY -------------------------------------------- 3409 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1651 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93037 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 370.4 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 8:05 PROSECTOR: LYNN HORNER RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3410 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1652 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93038 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:57 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 53; 115 X 115MM; VERIFIED AT NECROPSY: -HYPERTROPHY, HEPATOCELLULAR, OBSERVED PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT LN, MESENTERIC (MS) : -HYPERPLASIA, RETICULOENDOTHELIAL,- SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM -B-ADENOMA,-NEOPLASM PRESENT 3411 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1653 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93038 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:57 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MOTTLED; BROWN, TAN AND DARK RED SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL THORACIC -CYST / GALACTOCELE,-PRESENT REGION: SINGLE MASS, 110 X 85 X 55 MM >NOTE:>SEE SKIN FOR DIAGNOSIS (292.5 GRAMS), TAN, RED, SOFT, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) UTERUS (UT) : -CYST,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL INADVERTENTLY SACRIFICED WITH SODIUM PENTOBARBITAL -------------------------------------------- 3412 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1654 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93038 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:57 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3413 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1655 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93039 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 587.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 66; 70 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -VACUOLATION, HEPATOCELLULAR, MULTIPLE DARK RED AREAS, UP TO 4 MM IN PERIPORTAL,-SLIGHT DIAMETER; COLLECTED ON ROUTINE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / SECTIONS EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -UNILATERALLY EXAMINED,-PRESENT FILLED CYST, 3 MM IN DIAMETER LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3414 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1656 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93039 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 587.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, TAN, AND -B-ADENOMA,-NEOPLASM PRESENT SOFT SKIN (SK) : SKIN (SK) : >NOTE:>SKIN ROUTINE SECTION TAKEN FROM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL RIGHT INGUINAL REGION DUE TO MAMMARY MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 80 X 70 X 40 MM, RED, -HYPERPLASIA, GLANDULAR,-MINIMAL TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>MAMMARY ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MAMMARY MASS UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE TO 4 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3415 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1657 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93039 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 587.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3416 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1658 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93040 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 296.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 20 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: OBSERVED PERIPORTAL,-SLIGHT >BEHAVIOR; ATAXIC; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 28 X 23 X 14 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3417 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1659 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93040 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 296.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3418 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1660 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93041 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 375.9 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 73; 10 X 10MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT NOT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; RED HAIR COAT; LATERAL- LIVER (LI) : THORACIC-LEFT; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; RED HAIR COAT; LATERA- PERIPORTAL,-MINIMAL RIGHT; VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL >MASS(ES); AXILLARY REGION-RIGHT; WEEK KIDNEY (KD) : 82; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SEVERE >APPEARANCE; HEAD TILT; LEFT; VERIFIED -HYPERPLASIA, TRANSITIONAL CELL,- AT NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT >BEHAVIOR; ATAXIC; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE INCREASED,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT 3419 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1661 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93041 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 375.9 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 10 MM IN DIAMETER, TAN, -CYST / GALACTOCELE,-PRESENT BROWN, RED AND FIRM; SAME ON CUT -HYPERPLASIA, GLANDULAR,-MINIMAL SURFACE, COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-SEVERE CLEAR FLUID -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE ABDOMINAL AND DORSAL THORACIC REGIONS: DRY RED MATERIAL -------------------------------------------- 3420 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1662 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93041 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 375.9 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3421 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1663 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 79; 70 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -I-CARCINOMA,-FROM PITUITARY 79; 10 X 10MM; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -CYST(S); VISCERAL SURFACE OF LEFT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK LATERAL LOBE: SINGLE CLEAR FLUID -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL 82; 10 X 10MM; VERIFIED AT NECROPSY: FILLED CYST, 1 MM IN DIAMETER; -HYPERPLASIA, BILE DUCT,-MINIMAL OBSERVED COLLECTED ON ROUTINE SECTION -FIBROSIS, PERIBILIARY,-SLIGHT >SKIN & PELAGE; SORE/SCAB; AXILLARY KIDNEY (KD) : REGION-LEFT; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL >MASS(ES); DORSAL; WEEK 92; 30 X 30MM; -MINERALIZATION, PELVIC,-SLIGHT VERIFIED AT NECROPSY: OBSERVED SPLEEN (SP) : >RESPIRATION; AUDIBLE; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE INCREASED,-MODERATE >BEHAVIOR; HYPOACTIVE; VERIFIED AT PANCREAS (PA) : NECROPSY: NOT APPLICABLE -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); RIGHT: TWO -ANGIECTASIS,-MODERATE DARK RED AND SLIGHTLY RAISED AREAS, 3 -PIGMENT,-SLIGHT X 2 X 1 MM -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT 3422 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1664 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, EACH IS 3 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, -M-CARCINOMA,-NEOPLASM PRESENT DARK BROWN, TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,- 5 NEOPLASMS PRESENT SINGLE MASS, 15 X 15 X 8 MM, DARK RED, -CYST / GALACTOCELE,-PRESENT TAN, LOBULATED AND FIRM; SAME ON CUT -INFLAMMATION, CHRONIC-ACTIVE,- SURFACE (CAP 21); OVERLYING SKIN HAS A MODERATELY SEVERE SINGLE TAN AND DRY CRUSTED AREA, 8 X 5 -HYPERPLASIA, GLANDULAR,-MODERATE MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 22) 3423 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1665 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -THICKENED; VENTRAL CERVICAL REGION: TAN AND FIRM (CAP 23) -MASS #2; LEFT PERINEAL REGION: SINGLE MASS, 70 X 45 X 25 MM, DARK RED, TAN AND FIRM; ON SECTION EXUDES A DARK RED FLUID (CAP 24); REGIONAL LN (SEE INGUINAL) -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 25 X 15 MM, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN AND CREAMY SEMI- FLUID (CAP 25); REGIONAL LN (SEE INGUINAL) -MASS #4; RIGHT INGUINAL REGION, CRANIAL TO MASS #3: SINGLE MASS, 17 X 14 X 9 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (SEE INGUINAL) -MASS #5; DORSAL THORACIC REGION: SINGLE MASS, 15 MM IN DIAMETER, DARK RED, BROWN AND FIRM; ON SECTION EXUDES A DARK BROWN FLUID (CAP 28); REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL CERVIX (CV) : -EXUDATE, LUMINAL,-PRESENT 3424 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1666 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; LEFT: UP TO 12 X 8 X 7 MM; >UNREMARKABLE RIGHT IS NORMAL (CAP 26) >NOTE:>MAMMARY NEOPLASM ALSO ON SLIDE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3425 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1667 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3426 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1668 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 68; 100 X 70MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK -LARGE; ALL LOBES: MARGINS APPEAR -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 73; 20 X 20MM; VERIFIED AT NECROPSY: ROUNDED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC EOSINOPHILIC CELL,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL; WEEK 83; SURFACE OF RIGHT MEDIAN LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, 40 X 35MM; VERIFIED AT NECROPSY: DARK RED AREA, 3 X 2 MM; COLLECTED ON BASOPHILIC,-MINIMAL OBSERVED ROUTINE SECTION -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK -HYPERPLASIA, BILE DUCT,-MINIMAL 84; 50 X 55MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); AXILLARY REGION-LEFT; WEEK MINIMAL 95; 15 X 15MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT OBSERVED SPLEEN (SP) : >SKIN & PELAGE; SORE/SCAB; AXILLARY -HEMATOPOIESIS, EXTRAMEDULLARY, REGION-LEFT; VERIFIED AT NECROPSY: INCREASED,-MODERATE OBSERVED ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE 3427 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1669 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN >UNREMARKABLE OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR >UNREMARKABLE FLUID FILLED CYST ON EACH, EACH IS 1 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN, LOBULATED, AND FIRM RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 5 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT 3428 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1670 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL -B-FIBROADENOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 23 X 22 X 17 MM, -HYPERPLASIA, GLANDULAR,-SLIGHT RED, DARK RED, BROWN, TAN, LOBULATED, >NOTE:>MASS #3 IS THE FIBROADENOMA AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 65 X 55 X 49 MM, RED, DARK RED, TAN, FIRM, VASCULAR, AND LOBULATED; ON CUT SURFACE EXUDES A BROWN FLUID; PORTION COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) -MASS #3; RIGHT AXILLARY REGION EXTENDING TO RIGHT INGUINAL REGION: SINGLE MASS, 120 X 75 X 70 MM, RED, TAN, FIRM, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #4; LEFT AXILLARY REGION: SINGLE MASS, 20 X 15 X 11 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY CRUSTED AREA, 4 X 3 MM ;COLLECTED INTACT WITH MASS 3429 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1671 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #5; PERINEAL REGION: SINGLE MASS, 43 X 32 X 22 MM, TAN, FIRM, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP SEVERE TO 17 X 10 X 10 MM -MASS(ES); RIGHT HORN WITHIN WALL: SINGLE MASS, DARK RED AND SOFT, 8 X 5 X 5 MM, SAME ON CUT SURFACE; REGIONAL LN (SEE LUMBAR) CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: 11 X 7 X 2 MM (RIGHT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,- 7 X 4 X 1 MM) (CAP 24) MODERATELY SEVERE LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; RIGHT: 14 X 10 X 10 MM (LEFT >TISSUE MISSING NOT IDENTIFIED) (CAP 33) >NOTE:>TISSUE IS MAMMARY GLAND WITH CARCINOMA 3430 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1672 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HYPERPLASIA, LYMPHOPLASMACYTIC,- 34) MODERATE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3431 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1673 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3432 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1674 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93044 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 308.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:37 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; HEAD-ENTIRE; SPLEEN (SP) : VERIFIED AT NECROPSY: OBSERVED -PIGMENT, INCREASED,-MODERATE >APPEARANCE; MALOCCLUSION; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -ANGIECTASIS,-SLIGHT >BEHAVIOR; HYPOACTIVE; MODERATE; -PIGMENT,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE OVARY (OV) : >EXCRETION; FEW FECES; VERIFIED AT -CYST,-PRESENT NECROPSY: NOT APPLICABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN, SOFT AND LOBULATED STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); DORSAL SURFACE OF -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT MUZZLE: SINGLE DARK RED, DRY CRUSTED -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE AREA, 3 X 2 MM (CAP 20) -SUPERFICIAL CRUSTING,-MODERATELY SEVERE 3433 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1675 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93044 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 308.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:37 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM; COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-MODERATE -CYST(S); ALL REGIONS, DIFFUSE: -B-ADENOMA,-NEOPLASM PRESENT MULTIPLE TAN CREAMY SEMIFLUID FILLED -INFLAMMATION, CHRONIC,-MODERATE CYSTS, PINPOINT AND UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN -------------------------------------------- 3434 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1676 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93044 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 308.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:37 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3435 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1677 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93045 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 221.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:15 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.6925 3.9251 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3436 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1678 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93045 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 221.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:15 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3437 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1679 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93046 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : 58; 90 X 90MM; VERIFIED AT NECROPSY: -HYPERTROPHY, HEPATOCELLULAR, OBSERVED PERIPORTAL,-MODERATE >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK -MITOSIS, HEPATOCELLULAR, INCREASED,- 64; 30 X 25MM; VERIFIED AT NECROPSY: MINIMAL OBSERVED KIDNEY (KD) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -NEPHROPATHY, CHRONIC PROGRESSIVE,- CERVICAL-LEFT; VERIFIED AT NECROPSY: MINIMAL OBSERVED -MINERALIZATION, PELVIC,-MINIMAL >MASS(ES); INGUINAL REGION-LEFT; WEEK SPLEEN (SP) : 73; 10 X 10MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY, OBSERVED INCREASED,-SLIGHT >APPEARANCE; PROLAPSED; VAGINA; THYMUS (TH) : VERIFIED AT NECROPSY: OBSERVED -DEPLETION, LYMPHOCYTIC (GENERALLY >DISCHARGE; GENITAL; RED IN COLOR; COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- VERIFIED AT NECROPSY: OBSERVED MINIMAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; PITUITARY (PI) : VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>MASS #1 CONTAINS BOTH FIBROADENOMA AND CARCINOMA 3438 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1680 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93046 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 41 X 25 X 20 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21); OVERLYING SKIN HAS A SINGLE DRY, BROWN CRUSTED AREA, 7 X 4 MM; COLLECTED WITH MASS -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 100 X 80 X 36 MM, RED, GREY, TAN, LOBULATED, VASCULAR AND FIRM; SOME SECTIONS EXUDE A GREEN CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 12 X 10 X 5 MM, RED, GREY, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT VAGINA (VA) : -B-POLYP, STROMAL,-NEOPLASM PRESENT 3439 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1681 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93046 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, 32 X 12 X 12 MM, DARK RED, GELATINOUS AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23); POSSIBLY CONSISTENT WITH PROLAPSE (VAGINA) AS NOTED IN CLINICAL OBSERVATIONS CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT >NOTE:>THE FIBROADENOMA IS THE CAUSE OF DEATH LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERINEUM REGION: DARK RED AND MOIST MATERIAL -GI TRACT-DARK FLUID CONTENT; STOMACH: BLACK SEMIFLUID MATERIAL -------------------------------------------- 3440 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1682 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93046 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3441 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1683 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93047 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 864.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:52 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; OBES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SURFACE OF LEFT LATERAL LOBE: SINGLE -VACUOLATION, HEPATOCELLULAR, TAN AREA, 4 X 3 MM; COLLECTED ON PERIPORTAL,-SLIGHT ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / -RED FOCUS(I)/AREA(S); RIGHT MEDIAN EOSINOPHILIC CELL,-MINIMAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 -FOCUS, ALTERED HEPATOCELLULAR, X 2 MM; COLLECTED ON ROUTINE SECTION BASOPHILIC,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3442 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1684 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93047 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 864.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:52 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -INFLAMMATION, ACUTE,-SLIGHT MULTIPLE TAN AREAS, UP TO 4 X 3 MM -EROSION,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT ABDOMINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 30 X 25 X 15 MM, RED, -HYPERPLASIA, GLANDULAR,-MODERATE TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 3443 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1685 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93047 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 864.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:52 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3444 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1686 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93048 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : CERVIX (CV) : -LARGE; 10 MM IN DIAMETER -HYPERPLASIA, STROMAL,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3445 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1687 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93048 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3446 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1688 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93049 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:20 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: NOT APPLICABLE -CYST(S); LEFT, CORTICAL SURFACE: -CYST,-PRESENT >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED SINGLE CLEAR FLUID FILLED CYST, 2 MM AT NECROPSY: NOT APPLICABLE IN DIAMETER >BEHAVIOR; ATAXIC; MODERATE; VERIFIED SPLEEN (SP) : AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 8 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3447 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1689 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93049 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:20 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY AND RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3448 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1690 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93050 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 14:25 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 76; 50 X 50MM; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED MULTIPLE RED AND DARK RED AREAS, UP TO BASOPHILIC,-SLIGHT >MASS(ES); INGUINAL REGION-RIGHT; WEEK 3 MM IN DIAMETER; COLLECTED WITH -HYPERPLASIA, BILE DUCT,-MINIMAL 79; 65 X 60MM; VERIFIED AT NECROPSY: ROUTINE SECTIONS -HYPERTROPHY, HEPATOCELLULAR, OBSERVED PERIPORTAL,-MODERATE >EXCRETION; NONFORMED FECES; VERIFIED -CYST, BILIARY,-PRESENT AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: OBSERVED MINIMAL >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, TUBULAR,-SLIGHT NECROPSY: OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MODERATE -PIGMENT,-MINIMAL 3449 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1691 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93050 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 14:25 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE PITUITARY (PI) : PITUITARY (PI) : -CYST(S); MULTIPLE CYSTS, UP TO 2 MM -B-ADENOMA,-NEOPLASM PRESENT IN DIAMETER, FILLED WITH A DARK RED FLUID MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 35 X 45 X 55 MM, FIRM, -CYST / GALACTOCELE,-PRESENT RED, TAN AND LOBULATED; ON SECTION -B-FIBROADENOMA,-NEOPLASM PRESENT SOME AREAS CONTAIN A TAN SEMIFLUID; -HYPERPLASIA, GLANDULAR,-MODERATE COLLECTED; REGIONAL LN (INGUINAL) NOT >NOTE:>MASS #1 IS THE FIBROADENOMA IDENTIFIED -MASS #2; RIGHT INGUINAL REGION, CAUDAL TO MASS 1, EXTENDS INTO PERIANAL REGION: SINGLE MASS, 45 X 55 X 70 MM, FIRM, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CERVIX (CV) : CERVIX (CV) : -LARGE; 11 X 13 X 18 MM, FIRM AND -HYPERPLASIA, STROMAL,-SEVERE WHITE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT 3450 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1692 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93050 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 14:25 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; RIGHT: DARK RED (CAP -HYPERPLASIA, LYMPHOPLASMACYTIC,- 21) MODERATE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3451 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1693 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93050 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 14:25 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3452 Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume IX of IX Page 3453 of 4068 Covance 6329-183 3M T-6295 VOLUME IX OF IX CONTENTS Page APPENDIX 6 (Continued) ........................................................................................ 3455 Individual Anatomic Pathology Data ..................................................................... 3456 APPENDIX 7............................................................................................................ 4004 Cell Proliferation Report ....................................................................................... 4005 APPENDIX 8............................................................................................................ 4029 Electron Microscopy Report .................................................................................. 4030 3454 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 3455 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1694 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93051 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:14 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0353 .0147 % .0164 WEIGHT TAKEN RT ADRENAL (AD1) .0352 .0147 % .0163 WEIGHT TAKEN BRAIN (BR) 2.1584 .9008 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.0982 .4583 % .5088 WEIGHT TAKEN RT KIDNEY (KD1) 1.1061 .4616 % .5125 WEIGHT TAKEN LIVER (LI) 7.8763 3.2873 % 3.6491 WEIGHT TAKEN LUNG (LU) 1.4057 .5867 % .6513 WEIGHT TAKEN LF OVARY (OV0) .0840 .0351 % .0389 WEIGHT TAKEN RT OVARY (OV1) .0747 .0312 % .0346 WEIGHT TAKEN SPLEEN (SP) .4849 .2024 % .2247 WEIGHT TAKEN LF THYROID/PARA (TT0) .0101 .0042 % .0047 WEIGHT TAKEN RT THYROID/PARA (TT1) .0106 .0044 % .0049 WEIGHT TAKEN UTERUS (UT) 1.4870 .6206 % .6889 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3456 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1695 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93051 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:14 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3457 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1696 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93052 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 395.4 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:07 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; LEFT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYE-LEFT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; COLD TO TOUCH; LIMBS-ALL; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL BONE, FEMUR (FE) : BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TRIMMING MARROW, FEMUR (FM) : MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: MISSING AT >TISSUE MISSING TRIMMING PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT BROWN, LOBULATED, AND SOFT 3458 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1697 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93052 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 395.4 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:07 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-SLIGHT AND FIRM; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, DRY MATERIAL -ANIMAL OBESE -------------------------------------------- 3459 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1698 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93052 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 395.4 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:07 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3460 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1699 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93053 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 334.9 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 14:37 PROSECTOR: LYNN HORNER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 42; 70 X 55MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT 3461 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1700 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93053 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 334.9 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 14:37 PROSECTOR: LYNN HORNER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 75 X 60 X 23 MM, RED, TAN, GRAY, VASCULAR, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE, TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3462 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1701 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93053 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 334.9 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 14:37 PROSECTOR: LYNN HORNER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3463 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1702 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93054 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 10:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 71; 30 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT >APPEARANCE; THIN; VERIFIED AT -FIBROSIS, PERIBILIARY,-MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : >BEHAVIOR; ATAXIC; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -PIGMENT, INCREASED,-SLIGHT AT NECROPSY: NOT APPLICABLE ADRENAL, CORTEX (AC) : >EXCRETION; FEW FECES; VERIFIED AT -DEGENERATION, CYSTIC / HEMORRHAGIC,- NECROPSY: NOT APPLICABLE MODERATE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT FIRM 3464 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1703 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93054 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 10:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 30 X 20 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3465 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1704 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93054 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 10:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3466 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1705 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93055 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 508.0 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 15:17 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EXCRETION; FEW FECES; VERIFIED AT EYE (EY) : NECROPSY: NOT APPLICABLE -DYSTROPHY, CORNEAL IN EARLY STAGES, >SKIN & PELAGE; RED HAIR COAT; HEAD- CONSISTS PRIMARILY OF FOCAL ENTIRE; VERIFIED AT NECROPSY: OBSERVED MINERALIZATION,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SEVERE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM, RIGHT IS 3 -ANGIECTASIS,-MODERATELY SEVERE MM IN DIAMETER -RED FOCUS(I)/AREAS(I); LEFT: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3467 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1706 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93055 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 508.0 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 15:17 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 4 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -DILATATION,-MODERATE -CYST(S); LEFT WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 5 X 3 X 3 MM VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT TAN, CREAMY SEMIFLUID CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; HEAD: RED DRY MATERIAL -------------------------------------------- 3468 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1707 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93055 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 508.0 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 15:17 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3469 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1708 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93056 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 95; 25 X 25MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL; WEEK 99; -MASS(ES); RIGHT MEDIAN LOBE, NEAR -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 20 X 20MM; VERIFIED AT NECROPSY: HILUS: SINGLE MASS, 7 X 5 X 5 MM, TAN, -VACUOLATION, HEPATOCELLULAR, OBSERVED BROWN, AND SOFT; SAME ON CUT SURFACE; PERIPORTAL,-MINIMAL >APPEARANCE; THIN; VERIFIED AT COLLECTED; REGIONAL LN (HEPATIC) NOT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED IDENTIFIED BASOPHILIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL NECROPSY: NOT APPLICABLE -B-CHOLANGIOMA,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: MULTIPLE CLEAR FLUID >UNREMARKABLE FILLED CYSTS, UP TO 2 MM IN DIAMETER 3470 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1709 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93056 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT >SECTION EXAMINED; TISSUE NOT PRESENT IDENTIFIED, POSSIBLE REMNANTS COLLECTED PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID PERIANAL REGION: SINGLE -M-CARCINOMA,- 2 NEOPLASMS PRESENT MASS, 30 X 25 X 15 MM, DARK RED, RED, -CYST / GALACTOCELE,-PRESENT TAN, BROWN, AND SOFT; SAME ON CUT -B-FIBROADENOMA,-NEOPLASM PRESENT SURFACE; COLLECTED; POSSIBLY -HYPERPLASIA, GLANDULAR,-MODERATE CONSISTENT WITH MASS (VENTRAL >NOTE:>FIBROADENOMA IS ON SLIDE #19 ABDOMINAL) NOTED IN CLINICAL MASSES 1 AND 2 ARE CARCINOMAS OBSERVATION; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 35 X 25 X 20 MM, DARK RED, TAN, AND FIRM, CUT SURFACE EXUDES A DARK REDDISH-BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP SLIGHT TO 2 MM IN DIAMETER 3471 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1710 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93056 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3472 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1711 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93056 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3473 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1712 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93057 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 377.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 11:30 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 68; 75 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -HEMORRHAGE,-MODERATE >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; -I-CARCINOMA,-NEOPLASM PRESENT 15 X 15MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED BASOPHILIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT APPLICABLE -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 6 X 3 MM (RIGHT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATELY X 4 X 2 MM) SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 3474 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1713 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93057 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 377.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 11:30 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, DARK -M-CARCINOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 30 X 25 X 20 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, AND FIRM; CUT SURFACE -HYPERPLASIA, GLANDULAR,-MINIMAL EXUDES A DARK RED SEMIFLUID; >NOTE:>MASS #2 IS THE FIBROADENOMA COLLECTED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL) NOTED IN CLINICAL OBSERVATION; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 80 X 60 X 50 MM, TAN, RED, DARK RED, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF RIGHT -B-POLYP, ENDOMETRIAL, STROMAL,- DISTAL HORN: SINGLE MASS, 12 X 4 X 4 NEOPLASM PRESENT MM, TAN, RED, AND SOFT; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3475 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1714 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93057 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 377.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 11:30 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3476 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1715 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93058 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 235.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:38 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.6075 3.2293 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3477 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1716 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93058 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 235.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:38 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3478 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1717 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93059 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:40 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 3479 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1718 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93059 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:40 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3480 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1719 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93059 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:40 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3481 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1720 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93060 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 473.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:10 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK BIFURCATION OF MEDIAN LOBES: SINGLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- 93; VERIFIED AT NECROPSY: OBSERVED YELLOW AREA, 4 X 3 MM (CAP 22) MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 5 -ANGIECTASIS,-SLIGHT X 4 X 3 MM) -HYPERTROPHY, CORTICAL CELL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE 3482 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1721 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93060 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 473.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:10 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID LEFT ASPECT: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -HYPERPLASIA, GLANDULAR,-MINIMAL SINGLE MASS, 18 X 12 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 3483 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1722 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93060 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 473.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:10 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3484 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1723 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93061 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 8:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; PALE; ENTIRE BODY; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-SLIGHT NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : -CYST(S); RIGHT, INVOLVING ENTIRE -NEPHROPATHY, CHRONIC PROGRESSIVE,- ORGAN: SINGLE CLEAR FLUID FILLED CYST, SEVERE 40 MM IN DIAMETER -DEGENERATION, CYSTIC, UNILATERAL,- -ROUGH SURFACE, DIFFUSE; LEFT SEVERE -LARGE; LEFT: 28 X 17 X 17 MM (RIGHT SEE CYST COMMENT) SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 6 X 4 MM (LEFT IS 4 -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL X 3 X 2 MM) -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE 3485 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1724 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93061 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 8:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 3 X 3 -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MM -DIFFUSELY LIGHT; BILATERAL: TAN PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,- SLIGHT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 3486 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1725 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93061 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 8:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; LEFT AND RIGHT INGUINAL -CYST / GALACTOCELE,-PRESENT REGIONS: TAN AND FIRM; COLLECTED ON -B-FIBROADENOMA,-NEOPLASM PRESENT ROUTINE SECTION -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>SEE SLIDE #24 UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT, WITHIN WALL: SINGLE -DILATATION,-MODERATELY SEVERE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT -HEMORRHAGE,-PRESENT IS 3 X 2 X 1 MM) (CAP 24) -INFILTRATE, MACROPHAGE, PIGMENTED,- -DIFFUSELY RED; BILATERAL: DARK RED MODERATE LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 9 X 4 X 4 -HEMORRHAGE,-PRESENT MM, RIGHT IS 4 X 3 X 3 MM (CAP 23) -INFILTRATE, MACROPHAGE, PIGMENTED,- -DIFFUSELY RED; LEFT: DARK RED (CAP SLIGHT 23) AORTA (AO) : AORTA (AO) : -LARGE; DIFFUSE: UP TO 4 MM IN -MINERALIZATION,-SLIGHT DIAMETER (CAP 21) -FIRM; DIFFUSE (CAP 21) URETER (UE) : URETER (UE) : -LARGE; BILATERAL: UP TO 3 MM IN >UNREMARKABLE DIAMETER (CAP 22) 3487 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1726 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93061 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 8:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -GI TRACT-DARK DRY CONTENT; STOMACH: FILLED WITH A BROWN CLOTH-LIKE MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3488 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1727 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93062 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 5 X 4 X 3 MM (LEFT IS 4 -HYPERPLASIA, C-CELL,-SLIGHT X 2 X 2 MM) -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 3489 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1728 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93062 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 3490 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1729 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93062 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3491 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1730 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93063 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -I-CARCINOMA,-FROM PITUITARY >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 100; 40 X 30MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >MASS(ES); INGUINAL REGION-RIGHT; WEEK CENTRILOBULAR POLYKARYOCYTOSIS 101; VERIFIED AT NECROPSY: OBSERVED OCCASIONALLY NOTED,-SLIGHT >MASS(ES); VENTRAL-CERVICAL; WEEK 102; -GRANULAR CYTOPLASM, EOSINOPHILIC, 35 X 35MM; VERIFIED AT NECROPSY: CENTRILOBULAR,-SLIGHT OBSERVED -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; SORE/SCAB; AXILLARY PERIPORTAL,-MINIMAL REGION-RIGHT; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED BASOPHILIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, DARK RED, -M-CARCINOMA,-NEOPLASM PRESENT TAN, AND SOFT 3492 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1731 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93063 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 50 X 40 X 30 MM, RED, -CYST / GALACTOCELE,-PRESENT TAN, AND FIRM; SAME ON CUT SURFACE; -B-FIBROADENOMA,-NEOPLASM PRESENT SKIN OVERLYING MASS HAS A MOIST DARK -HYPERPLASIA, GLANDULAR,-SLIGHT BROWN AND RED ULCERATED AREA, 25 X 15 -B-ADENOMA,-NEOPLASM PRESENT MM; COLLECTED; REGIONAL LN (AXILLARY) >NOTE:>MASS 1 IS A CARCINOMA MASS 2 IS NORMAL (CAP 22) A FIBROADENOMA -MASS #2; RIGHT CERVICAL REGION: SINGLE MASS, 45 X 30 X 20 MM, TAN, LOBULATED, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 24) -MASS #3; RIGHT ABDOMINAL REGION: SINGLE MASS, 15 X 15 X 7 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE 3493 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1732 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93063 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; RIGHT: DRY DARK RED MATERIAL -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3494 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1733 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93064 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0300 .0124 % .0148 WEIGHT TAKEN RT ADRENAL (AD1) .0306 .0126 % .0151 WEIGHT TAKEN BRAIN (BR) 2.0301 .8372 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2222 .5040 % .6020 WEIGHT TAKEN RT KIDNEY (KD1) 1.1563 .4768 % .5696 WEIGHT TAKEN LIVER (LI) 9.0107 3.7158 % 4.4385 WEIGHT TAKEN LUNG (LU) 1.3975 .5763 % .6884 WEIGHT TAKEN LF OVARY (OV0) .0856 .0353 % .0422 WEIGHT TAKEN RT OVARY (OV1) .0768 .0317 % .0378 WEIGHT TAKEN SPLEEN (SP) .4442 .1832 % .2188 WEIGHT TAKEN LF THYROID/PARA (TT0) .0098 .0040 % .0048 WEIGHT TAKEN RT THYROID/PARA (TT1) .0076 .0031 % .0037 WEIGHT TAKEN UTERUS (UT) .6495 .2678 % .3199 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DILATATION, PELVIC,-SLIGHT -GRANULAR MATERIAL; BILATERAL: -MINERALIZATION, PELVIC,-MINIMAL MULTIPLE WHITE PINPOINT GRANULES SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3495 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1734 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93064 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3496 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1735 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93065 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 12:35 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 83; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL WEEK 86; 10 X 10MM; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); AXILLARY REGION-LEFT; WEEK MINIMAL 87; VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT >RESPIRATION; AUDIBLE; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -FIBROSIS,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); LEFT: SINGLE -ANGIECTASIS,-MINIMAL DARK RED AREA, 4 X 3 MM -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3497 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1736 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93065 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 12:35 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT -HYPERPLASIA, EPITHELIAL,-MODERATE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN -INFILTRATE, MACROPHAGE, ALVEOLAR,- OF LEFT LOBE: SINGLE GREEN AREA, 5 X 4 MODERATE MM -INFLAMMATION, GRANULOMATOUS,-SLIGHT -HYPERPLASIA, TYPE II PNEUMOCYTE,- SLIGHT >NOTE:>SOME GRANULOMAS CONTAIN FOREIGN MATERIAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 7 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY FLUID FILLED -HYPERPLASIA, GLANDULAR,-MODERATE CYSTS, UP TO 20 X 15 X 4 MM; COLLECTED ON ROUTINE SECTION; POSSIBLY CONSISTENT WITH ALL MASSES NOTED IN CLINICAL OBSERVATIONS VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT 3498 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1737 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93065 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 12:35 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3499 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1738 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93066 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:03 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : LIVER (LI) : 54; 110 X 110MM MASSES ON LEFT SIDE -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ARE MEASURED AS ONE MASS; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: OBSERVED BASOPHILIC,-SLIGHT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -HYPERPLASIA, BILE DUCT,-MINIMAL WEEK 70; 35 X 35MM; VERIFIED AT -FIBROSIS, PERIBILIARY,-MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -NEPHROPATHY, CHRONIC PROGRESSIVE,- 84; 30 X 20MM; VERIFIED AT NECROPSY: MINIMAL OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- SPLEEN (SP) : SPLEEN (SP) : ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -LARGE; 51 X 12 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, OBSERVED INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE 3500 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1739 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93066 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:03 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 7 X 3 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); ALL REGIONS, DIFFUSE: MULTIPLE RED AREAS, UP TO 3 X 2 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 30 X 25 MM, TAN, -CYST / GALACTOCELE,-PRESENT RED, GREEN, VASCULAR AND FIRM; SAME ON -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE CUT SURFACE; COLLECTED INTACT; -B-FIBROADENOMA,-NEOPLASM PRESENT OVERLYING SKIN HAS MULTIPLE RED MOIST >NOTE:>MASS #1 IS THE CARCINOMA MASS #3 ULCERATED AREAS, UP TO 11 MM IN IS AN INFLAMED GALACTOCELE DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) NOTED IN CLINICAL OBSERVATIONS -MASS #2; LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 70 X 50 X 39 MM, ALL ARE TAN, VASCULAR AND FIRM; SAME ON CUT SURFACES; ALL COLLECTED INTACT; REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATIONS 3501 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1740 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93066 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:03 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 25 X 15 X 15 MM, RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A SINGLE MOIST RED ULCERATED AREA, 3 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 10 X 3 X 2 -HYPERPLASIA, LYMPHOPLASMACYTIC,- MM (CAP 22) MODERATELY SEVERE LN, INGUINAL (IN) : LN, INGUINAL (IN) : -LARGE; RIGHT: 10 X 5 X 2 MM (LEFT NOT -HYPERPLASIA, LYMPHOPLASMACYTIC,- IDENTIFIED) (CAP 21) MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3502 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1741 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93066 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:03 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3503 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1742 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93067 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:45 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.1068 3.9061 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3504 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1743 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93067 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:45 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3505 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1744 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93068 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 10:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ALOPECIA; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / AT NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >APPEARANCE; THIN; VERIFIED AT -HYPERPLASIA, BILE DUCT,-MINIMAL NECROPSY: NOT OBSERVED -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 5 X 5 MM (RIGHT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATELY MM IN DIAMETER) SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-SLIGHT 3506 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1745 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93068 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 10:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL NERVE, SCIATIC (SN) : -DEGENERATION,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GREY, LOBULATED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ILEUM (IL) : >AUTOLYTIC SKIN (SK) : SKIN (SK) : -ALOPECIA-DIFFUSE; ENTIRE VENTRAL >UNREMARKABLE SURFACE; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT 3507 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1746 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93068 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 10:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 3508 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1747 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93068 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 10:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3509 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1748 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 86; 45 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -LARGE; ALL LOBES: MARGINS ROUNDED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL 87; 60 X 50MM; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: BROWN AND TAN -VACUOLATION, HEPATOCELLULAR,-SEVERE OBSERVED KIDNEY (KD) : >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK -MINERALIZATION, PELVIC,-MINIMAL 92; 40 X 35MM; VERIFIED AT NECROPSY: -MINERALIZATION, TUBULAR,-MINIMAL OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT >MASS(ES); INGUINAL REGION-LEFT; WEEK -THROMBUS,-PRESENT 93; 50 X 50MM; VERIFIED AT NECROPSY: SPLEEN (SP) : OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 10 X 7 X 5 -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL MM, RIGHT IS 7 X 5 X 5 MM -HYPERTROPHY, CORTICAL CELL,-SEVERE -MOTTLED; BILATERAL: RED, BROWN AND -DEGENERATION, CYSTIC / HEMORRHAGIC,- TAN SEVERE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3510 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1749 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 5 X 3 MM; DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 60 X 45 X 15 MM, RED, -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDES A TAN CREAMY SEMIFLUID (CAP 22); REGIONAL LN (RIGHT AXILLARY) NORMAL; LEFT NOT IDENTIFIED (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 65 X 39 X 15 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (RIGHT AXILLARY) NORMAL; LEFT NOT IDENTIFIED (CAP 21); CONSISTENT WITH MASSES (AXILLARY REGION RIGHT AND VENTRAL THORACIC RIGHT) NOTED IN CLINICAL OBSERVATIONS 3511 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1750 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 55 X 45 X 30 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3512 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1751 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3513 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1752 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93070 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:21 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.9068 4.1861 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3514 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1753 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93070 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:21 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3515 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1754 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93071 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 223.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:05 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0328 .0147 % .0167 WEIGHT TAKEN RT ADRENAL (AD1) .0269 .0120 % .0137 WEIGHT TAKEN BRAIN (BR) 1.9629 .8779 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) .9138 .4087 % .4655 WEIGHT TAKEN RT KIDNEY (KD1) .9168 .4100 % .4671 WEIGHT TAKEN LIVER (LI) 7.5052 3.3565 % 3.8235 WEIGHT TAKEN LUNG (LU) 1.2561 .5618 % .6399 WEIGHT TAKEN LF OVARY (OV0) .0551 .0246 % .0281 WEIGHT TAKEN RT OVARY (OV1) .0550 .0246 % .0280 WEIGHT TAKEN SPLEEN (SP) .4538 .2030 % .2312 WEIGHT TAKEN LF THYROID/PARA (TT0) .0091 .0041 % .0046 WEIGHT TAKEN RT THYROID/PARA (TT1) .0094 .0042 % .0048 WEIGHT TAKEN UTERUS (UT) 1.0898 .4874 % .5552 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT MISSING AT EMBEDDING ADRENAL, MEDULLA (MA) : ADRENAL, MEDULLA (MA) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT MISSING AT EMBEDDING 3516 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1755 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93071 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 223.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:05 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3517 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1756 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 87; 45 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -MINERALIZATION,-MINIMAL OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); INGUINAL REGION-LEFT; WEEK -LIGHT FOCUS(I)/AREA(S); MARGIN OF -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 103; 20 X 20MM; VERIFIED AT NECROPSY: RIGHT MEDIAN LOBE: SINGLE TAN FOCUS, 3 -GRANULAR CYTOPLASM, EOSINOPHILIC, OBSERVED X 5 MM; COLLECTED ON ROUTINE SECTION CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL 3518 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1757 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 5 X 4 MM (LEFT IS 5 -DEGENERATION, CYSTIC / HEMORRHAGIC,- X 3 X 2 MM) MINIMAL -PIGMENT,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 53 X 45 X 26, RED, TAN, -HYPERPLASIA, GLANDULAR,-SLIGHT FIRM, AND LOBULATED; SOME SECTIONS EXUDE A GREEN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 36 X 30 X 15 MM, RED, TAN, AND SOFT; SAME ON CUT SURFACE; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT 3519 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1758 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 5 X 4 X 3 MM CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3520 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1759 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3521 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1760 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93073 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/23/99 STUDY DAY OF DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/23/99 13:53 PROSECTOR: MICHELE MARGGI RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : NECROPSY: OBSERVED -M-ASTROCYTOMA,-NEOPLASM PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT -NECROSIS, INDIVIDUAL HEPATOCYTE,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >RESPIRATION; AUDIBLE; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -CONGESTION,-PRESENT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -CONGESTION,-PRESENT -MINERALIZATION, VASCULAR,-MINIMAL 3522 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1761 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93073 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/23/99 STUDY DAY OF DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/23/99 13:53 PROSECTOR: MICHELE MARGGI RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF LEFT HORN: -B-POLYP, ENDOMETRIAL, STROMAL,- SINGLE MASS, 10 X 5 X 5 MM, RED, DARK NEOPLASM PRESENT RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 21) VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT HEAD, CORONAL (HC) : -INFLAMMATION, ACUTE, EAR,-MINIMAL GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- 3523 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1762 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93073 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/23/99 STUDY DAY OF DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/23/99 13:53 PROSECTOR: MICHELE MARGGI RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3524 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1763 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93074 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 423.7 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:46 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -VACUOLATION, HEPATOCELLULAR, >BEHAVIOR; ATAXIC; VERIFIED AT PERIPORTAL,-MODERATE NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 X 5 X 5 MM (LEFT IS 4 -DEGENERATION, CYSTIC / HEMORRHAGIC,- X 2 X 2 MM) MODERATE -PIGMENT,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 3525 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1764 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93074 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 423.7 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:46 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 7 X 5 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM -RED FOCUS(I)/AREA(S); ALL REGIONS, DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: -ACANTHOSIS / HYPERKERATOSIS,-MODERATE MULTIPLE TAN SLIGHTLY RAISED AREAS, UP -EDEMA,-PRESENT TO 3 X 2 MM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -INFLAMMATION, CHRONIC,-SLIGHT LIGHT GREEN MUCOIDAL MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3526 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1765 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93074 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 423.7 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:46 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3527 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1766 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93075 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:46 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.6760 3.5171 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3528 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1767 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93075 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:46 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3529 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1768 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93076 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MISSING; TEETH; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >MASS(ES); LIMB-HIND-LEFT; WEEK 59; 120 -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL X 125MM; VERIFIED AT NECROPSY: MULTIPLE DARK RED AREAS, UP TO 3 X 2 -HYPERTROPHY, HEPATOCELLULAR, OBSERVED MM; SOME ARE SUNKEN WITH A STELLATE CENTRILOBULAR POLYKARYOCYTOSIS >MASS(ES); AXILLARY REGION-RIGHT; WEEK PATTERN; COLLECTED ON ROUTINE SECTIONS OCCASIONALLY NOTED,-MINIMAL 84; 50 X 50MM; VERIFIED AT NECROPSY: -NECROSIS, COAGULATIVE,-MINIMAL OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; THIN; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE NECROPSY: OBSERVED SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE 3530 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1769 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93076 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 130 X 100 X 40 MM, TAN, >NOTE:>FOR DIAGNOSIS OF MASS #1, SEE LOBULATED AND SOFT; ON SECTION EXUDES SKIN A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 70 X 50 X 30 MM, TAN, RED, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) 3531 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1770 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93076 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL LOWER INCISORS -ANIMAL THIN -------------------------------------------- 3532 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1771 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93076 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3533 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1772 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93077 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:21 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); TAIL-PROXIMAL; WEEK 97; 25 X BRAIN (BR) : BRAIN (BR) : 15MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 101; 30 X 30MM; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -ROUGH SURFACE, DIFFUSE; ALL LOBES -GRANULAR CYTOPLASM, EOSINOPHILIC, >SKIN & PELAGE; SORE/SCAB; TAIL- CENTRILOBULAR,-MINIMAL PROXIMAL; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, OBSERVED PERIPORTAL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : SPLEEN (SP) : -LARGE; 57 X 16 X 11 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT -HYPERPLASIA, LYMPHORETICULAR,-SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT 3534 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1773 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93077 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:21 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 10 X 9 MM (RIGHT IS -DEGENERATION, CYSTIC / HEMORRHAGIC,- 5 X 3 X 3 MM) SEVERE -PIGMENT,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 6 X 5 X 4 MM (LEFT IS 5 -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM X 2 X 2 MM) PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 39 X 27 X 14 MM, TAN, -HYPERPLASIA, GLANDULAR,-SLIGHT RED, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN WALLS OF LEFT HORN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATE TO 3 MM IN DIAMETER 3535 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1774 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93077 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:21 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : CERVIX (CV) : -CYST(S); WITHIN LUMEN: SINGLE RED -HYPERPLASIA, CYSTIC,-MODERATE FLUID FILLED CYST, 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT TAIL (TI) : TAIL (TI) : -MASS(ES); 25 MM FROM BASE: 15 MM IN -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE DIAMETER, RED, TAN, AND SOFT; ON -M-LIPOSARCOMA,-NEOPLASM PRESENT SECTION EXUDES A DARK RED FLUID; COLLECTED; REGIONAL LN (SEE LUMBAR); OVERLYING SKIN HAS A DARK RED MOIST ABRADED AREA, 5 MM IN DIAMETER; COLLECTED INTACT WITH MASS LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP -HYPERPLASIA, LYMPHOPLASMACYTIC,- 25) MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3536 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1775 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93077 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:21 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3537 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1776 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93078 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:58 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 89; 70 X 65MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 94; 40 X 40MM; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OBSERVED EOSINOPHILIC CELL,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK -HYPERPLASIA, BILE DUCT,-MINIMAL 104; VERIFIED AT NECROPSY: NOT KIDNEY (KD) : KIDNEY (KD) : OBSERVED -DIFFUSELY DARK; BILATERAL: DARK BROWN -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERTROPHY, CORTICAL CELL,-SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- SEVERE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 3 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER 3538 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1777 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93078 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:58 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AND RIGHT INGUINAL -CYST / GALACTOCELE,-PRESENT REGIONS: TWO MASSES, LEFT INGUINAL IS -B-FIBROADENOMA,-NEOPLASM PRESENT 50 X 46 X 30 MM, RIGHT INGUINAL IS 80 X 55 X 30 MM, BOTH ARE RED, TAN, SOFT AND LOBULATED; BOTH ARE SAME ON CUT SURFACE; BOTH ON SECTION EXUDE A TAN CREAMY SEMIFLUID; RIGHT INGUINAL COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3539 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1778 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93078 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:58 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3540 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1779 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93079 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 69; 70 X 80MM; VERIFIED AT NECROPSY: -LARGE; ALL LOBES: MARGINS APPEAR -VACUOLATION, HEPATOCELLULAR, OBSERVED ROUNDED PERIPORTAL,-MODERATE >MASS(ES); AXILLARY REGION-LEFT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 101; 10 X 10MM; VERIFIED AT NECROPSY: EOSINOPHILIC CELL,-MODERATE OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 90 X 75 X 40 MM, RED, -HYPERPLASIA, GLANDULAR,-SEVERE TAN, LOBULATED, FIRM AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 3541 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1780 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93079 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 20 X 17 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (AXILLARY) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3542 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1781 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93079 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3543 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1782 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93080 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:49 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED, -HYPERPLASIA,-MODERATE TAN, AND FIRM MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 3544 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1783 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93080 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:49 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3545 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1784 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93081 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -HYPERTROPHY, HEPATOCELLULAR, OF RIGHT AND LEFT MEDIAN LOBES: SINGLE CENTRILOBULAR POLYKARYOCYTOSIS TAN AREA, 5 X 2 MM (CAP 22) OCCASIONALLY NOTED,-MINIMAL -MASS(ES); LEFT LATERAL LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / MASS, 20 X 15 X 11 MM, RED, DARK RED, EOSINOPHILIC CELL,-MODERATE BROWN AND SOFT; SAME ON CUT SURFACE; -FOCUS, ALTERED HEPATOCELLULAR, COLLECTED; REGIONAL LN (HEPATIC) NOT BASOPHILIC,-MINIMAL IDENTIFIED -HYPERPLASIA, BILE DUCT,-SLIGHT -RED FOCUS(I)/AREA(S); RIGHT MEDIAN -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM LOBE: SINGLE DARK RED AREA, 2 X 1 MM; PRESENT COLLECTED ON ROUTINE SECTION -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 3 X 2 MM MODERATE MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -ATROPHY-FOCAL; CRANIAL ONE HALF: 10 X >UNREMARKABLE 4 X 2 MM 3546 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1785 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93081 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF LEFT HORN: -DILATATION,-MODERATELY SEVERE SINGLE MASS, 20 X 7 X 4 MM, RED, DARK -HEMORRHAGE,-PRESENT RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3547 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1786 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93081 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3548 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1787 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; PALE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); DORSAL SURFACE >UNREMARKABLE >SKIN & PELAGE; YELLOW SKIN; PAWS-ALL; OF BOTH CEREBRAL HEMISPHERES: MULTIPLE VERIFIED AT NECROPSY: OBSERVED DARK RED PINPOINT FOCI >SKIN & PELAGE; YELLOW SKIN; TAIL; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES: MARGINS ROUNDED, -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO >SKIN & PELAGE; YELLOW HAIR COAT; LOBES THICKENED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT VERIFIED AT NECROPSY: OBSERVED -MOTTLED; ALL LOBES: RED AND TAN -HEMORRHAGE,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -CYST, BILIARY,-PRESENT NECROPSY: NOT APPLICABLE MULTIPLE TAN SLIGHTLY RAISED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS -ROUGH SURFACE, DIFFUSE; ALL LOBES -CYST(S); ALL LOBES: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 22) KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -MINERALIZATION, PELVIC,-SLIGHT -PIGMENT, TUBULAR,-SEVERE SPLEEN (SP) : SPLEEN (SP) : -LARGE; 65 X 20 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, -ADHESION(S); BOTH SURFACES OF CAUDAL INCREASED,-MODERATE END: MULTIPLE TAN FIBROUS ADHESIONS; -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO COLLECTED INTACT NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3549 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1788 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 8 MM IN DIAMETER -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -DIFFUSELY RED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 2 MM IN DIAMETER THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE DARK RED AREAS, UP TO 5 X 4 NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MM -MINERALIZATION, VASCULAR,-MINIMAL -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR -HYPERPLASIA,-MINIMAL ASPECT: TWO DARK RED FOCI, ONE IS 2 MM IN DIAMETER, THE OTHER IS 1 MM IN DIAMETER STOMACH, GL (ST) : -EROSION,-MINIMAL 3550 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1789 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM ILEUM (IL) : ILEUM (IL) : -DIVERTICULUM(A); SINGLE DIVERTICULUM, -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 8 X 5 X 3 MM (CAP 24) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CECUM (CE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT COLON (CO) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 21) -HEMORRHAGE,-PRESENT LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 8 X 5 -HEMORRHAGE,-PRESENT X 3 MM (CAP 23) 3551 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1790 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) LN, ILEOCECAL (IC) : LN, ILEOCECAL (IC) : -LARGE; 8 X 5 X 3 MM (CAP 25) -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO -DIFFUSELY RED; (CAP 25) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL AND THORACIC REGIONS: YELLOW AND DRY MATERIAL -YELLOWISH MUCOUS MEMBRANES; ALL AREAS, INCLUDING EXTREMITIES -------------------------------------------- 3552 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1791 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT) ------------------------------------------------------------------------------------------------------------------------------------ 3553 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1792 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 87; 100 X 90MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL; WEEK 95; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 15 X 10MM; VERIFIED AT NECROPSY: NOT -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED BASOPHILIC,-MODERATELY SEVERE >APPEARANCE; THIN; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: OBSERVED PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 9 X 9 MM (RIGHT IS -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL 7 X 6 X 5 MM) -HYPERTROPHY, CORTICAL CELL,-SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3554 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1793 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY AND FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL EXTENDING -CYST / GALACTOCELE,-PRESENT TO VENTRAL THORACIC: SINGLE MASS, 120 -B-FIBROADENOMA,-NEOPLASM PRESENT X 110 X 50 MM (209.3 GRAMS), RED, DARK -HYPERPLASIA, GLANDULAR,-SLIGHT RED, TAN, GREY, LOBULATED FIRM, SOFT, AND VASCULAR; ON SECTION SOME AREAS EXUDE A RED FLUID, OTHERS EXUDE A TAN CASEOUS SEMISOLID; PORTION COLLECTED; OVERLYING SKIN HAS A DARK RED CRUSTED AREA, 65 X 35 MM; COLLECT INTACT WITH MASS; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) 3555 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1794 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3556 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1795 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3557 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1796 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93084 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:26 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 6.9911 3.7584 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3558 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1797 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93084 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:26 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3559 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1798 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93085 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 268.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:59 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0315 .0117 % .0147 WEIGHT TAKEN RT ADRENAL (AD1) .0337 .0126 % .0158 WEIGHT TAKEN BRAIN (BR) 2.1358 .7955 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1824 .4404 % .5536 WEIGHT TAKEN RT KIDNEY (KD1) 1.2842 .4783 % .6013 WEIGHT TAKEN LIVER (LI) 9.0653 3.3763 % 4.2445 WEIGHT TAKEN LUNG (LU) 1.1579 .4312 % .5421 WEIGHT TAKEN LF OVARY (OV0) .0745 .0277 % .0349 WEIGHT TAKEN RT OVARY (OV1) .0707 .0263 % .0331 WEIGHT TAKEN SPLEEN (SP) .4904 .1826 % .2296 WEIGHT TAKEN LF THYROID/PARA (TT0) .0080 .0030 % .0037 WEIGHT TAKEN RT THYROID/PARA (TT1) .0106 .0039 % .0050 WEIGHT TAKEN UTERUS (UT) .5732 .2135 % .2684 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3560 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1799 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93085 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 268.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:59 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3561 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1800 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93086 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:26 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 60 X 50MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED -DEGENERATION, CYSTIC,-MINIMAL AT NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT >EYES; DILATED PUPIL; EYES; VERIFIED AT -FIBROSIS, PERIBILIARY,-SLIGHT NECROPSY: NOT APPLICABLE KIDNEY (KD) : >BEHAVIOR; ATAXIC; VERIFIED AT -NEPHROPATHY, CHRONIC PROGRESSIVE,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -MINERALIZATION, PELVIC,-MODERATE NECROPSY: NOT APPLICABLE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 70 X 50 X 20 MM, RED, DARK RED, TAN, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3562 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1801 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93086 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:26 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3563 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1802 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93087 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/16/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS DATE AND TIME OF NECROPSY: 03/16/00 10:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 93; 40 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : 95; 20 X 20MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >EYES; RED DISCHARGE; EYES; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: OBSERVED OCCASIONALLY NOTED,-SLIGHT >DISCHARGE; NASAL; RED IN COLOR; -VACUOLATION, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED PERIPORTAL,-SLIGHT >EXCRETION; FEW FECES; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: NOT APPLICABLE BASOPHILIC,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL 3564 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1803 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93087 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/16/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS DATE AND TIME OF NECROPSY: 03/16/00 10:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 10 MM IN DIAMETER SPINAL CORD (SC) : -DEMYELINATION,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 40 X 35 X 25 MM, TAN, RED -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT AND SOFT; CUT SURFACE EXUDES A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 28 X 25 X 15 MM, TAN, RED AND FIRM; CUT SURFACE EXUDES A SMALL AMOUNT OF A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL 3565 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1804 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93087 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/16/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS DATE AND TIME OF NECROPSY: 03/16/00 10:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; -HYPERPLASIA, LYMPHOPLASMACYTIC,- COLLECTED MODERATE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY -------------------------------------------- 3566 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1805 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93087 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/16/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS DATE AND TIME OF NECROPSY: 03/16/00 10:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3567 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1806 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93088 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/15/99 STUDY DAY OF DEATH: 544 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/15/99 13:23 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; EYE (EY) : UNVERIFIED AT NECROPSY -DYSTROPHY, CORNEAL IN EARLY STAGES, >APPEARANCE; HEAD TILT; LEFT; CONSISTS PRIMARILY OF FOCAL UNVERIFIED AT NECROPSY MINERALIZATION,-SLIGHT >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; UNVERIFIED AT NECROPSY -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT MISSING FROM WET TISSUE -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : ADRENAL, MEDULLA (MA) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL -UNILATERALLY EXAMINED,-PRESENT MISSING FROM WET TISSUE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3568 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1807 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93088 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/15/99 STUDY DAY OF DEATH: 544 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/15/99 13:23 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 9 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, LOBULATED, AND SOFT ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATE MULTIPLE CLEAR FLUID FILLED CYSTS IN EACH, UP TO 8 MM IN DIAMETER CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS -------------------------------------------- 3569 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1808 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93088 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/15/99 STUDY DAY OF DEATH: 544 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/15/99 13:23 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3570 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1809 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -COMPRESSION, VENTRAL,-PRESENT 87; 15 X 15MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); VENTRAL-CERVICAL; WEEK 88; -HYPERTROPHY, HEPATOCELLULAR, 25 X 25MM; VERIFIED AT NECROPSY: CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED OCCASIONALLY NOTED,-SLIGHT >MASS(ES); AXILLARY REGION-RIGHT; WEEK -GRANULAR CYTOPLASM, EOSINOPHILIC, 92; 15 X 15MM; VERIFIED AT NECROPSY: CENTRILOBULAR,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; HEAD TILT; LEFT; VERIFIED BASOPHILIC,-MINIMAL AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -HYPERPLASIA, CORTICAL,-SLIGHT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; CAUDAL END OF CHAIN: -B-HEMANGIOMA,-NEOPLASM PRESENT DARK RED 3571 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1810 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 7 MM IN DIAMETER THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT COLON (CO) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 40 X 30 X 30 MM, RED, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT DARK RED, TAN, CYSTIC, FIRM, AND -HYPERPLASIA, GLANDULAR,-SLIGHT LOBULATED; SOME SECTIONS EXUDE A TAN -B-ADENOMA,-NEOPLASM PRESENT CREAMY SEMIFLUID; COLLECTED; REGIONAL >NOTE:>MASS #3 IS THE ADENOMA LN (MANDIBULAR) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 25 X 15 MM, RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) 3572 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1811 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT VENTRAL ABDOMINAL REGION: SINGLE MASS, 25 X 15 X 12 MM, DARK BROWN, TAN, AND FIRM; SOME SECTIONS EXUDE A BROWN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MULTIPLE CLEAR FLUID FILLED CYSTS, UP MODERATELY SEVERE TO 5 MM IN DIAMETER VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 3573 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1812 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3574 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1813 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93090 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 423.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:53 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL LOBE: MULTIPLE DARK FOCI, UP TO 3 MM -HYPERTROPHY, HEPATOCELLULAR, IN DIAMETER; COLLECTED ON ROUTINE CENTRILOBULAR POLYKARYOCYTOSIS SECTION OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-MODERATELY AND FIRM; COLLECTED ON ROUTINE SECTION SEVERE CERVIX (CV) : CERVIX (CV) : -LARGE; 12 X 8 X 5 MM -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE 3575 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1814 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93090 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 423.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:53 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, 7 X 5 X 3 MM, TAN, SOFT AND PEDUNCULATED; ON SECTION EXUDES A WHITE CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3576 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1815 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93090 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 423.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:53 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3577 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1816 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93091 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 13:16 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); LIMB-HIND-RIGHT; WEEK 47; BRAIN (BR) : 100 X 100MM; VERIFIED AT NECROPSY: -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >APPEARANCE; THIN; VERIFIED AT -ROUGH SURFACE, DIFFUSE; ALL LOBES -HEMATOPOIESIS, EXTRAMEDULLARY,- NECROPSY: OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN MODERATE >SKIN & PELAGE; SORE/SCAB; LATERAL- -DEGENERATION / NECROSIS, ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: CENTRILOBULAR,-MODERATE OBSERVED KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; PALE; ENTIRE BODY; -MOTTLED; BILATERAL: RED AND TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -VACUOLATION, TUBULAR EPITHELIUM,- MODERATE SPLEEN (SP) : SPLEEN (SP) : -LARGE; 55 X 15 X 8 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SEVERE LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -MOTTLED; DARK RED AND TAN -HEMORRHAGE,-PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 2 MM IN DIAMETER 3578 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1817 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93091 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 13:16 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, -N-CARCINOMA,-NEOPLASM PRESENT UP TO 7 X 7 X 6 MM, TAN AND FIRM; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED HEART (HT) : HEART (HT) : -LARGE; 25 X 15 X 11 MM >UNREMARKABLE -DIFFUSELY LIGHT; DIFFUSE: GRAY PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN AREA, 4 X 2 MM HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 3579 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1818 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93091 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 13:16 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT LATERAL LUMBAR REGION: SINGLE MASS, 98 X 75 X 68 MM, RED, DARK RED, TAN, FIRM, AND VASCULAR; ON SECTION EXUDES A DARK RED FLUID AND A YELLOW SEMIFLUID; PORTION COLLECTED; OVERLYING SKIN HAS A DARK RED MOIST ULCERATED AREA, 25 MM IN DIAMETER; COLLECTED INTACT WITH PORTION OF MASS COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: -DILATATION,-MODERATE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 X 3 X 3 MM CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK RED AND TAN >UNREMARKABLE (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3580 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1819 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93091 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 13:16 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3581 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1820 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93092 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 309.7 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 11:30 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK EYE (EY) : 72; 10 X 10MM; VERIFIED AT NECROPSY: -ATROPHY, RETINAL,-SLIGHT OBSERVED BRAIN (BR) : BRAIN (BR) : >APPEARANCE; THIN; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HEAD TILT; LEFT; VERIFIED LIVER (LI) : AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >EYES; RED DISCHARGE; EYES; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: OBSERVED OCCASIONALLY NOTED,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 6 X 5 MM (LEFT IS 6 -DEGENERATION, CYSTIC / HEMORRHAGIC,- X 4 X 2 MM) MODERATELY SEVERE -DARK FOCUS(I)/AREA(S); RIGHT CRANIAL -PIGMENT,-MINIMAL END: SINGLE DARK BROWN AREA, 5 MM IN DIAMETER 3582 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1821 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93092 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 309.7 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 11:30 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 9 X 9 MM, RED. DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GRAY, SOFT, AND LOBULATED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 28 X 20 X 13 MM, TAN AND -B-FIBROADENOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTIONS UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL >UNREMARKABLE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -ANIMAL THIN -------------------------------------------- 3583 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1822 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93092 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 309.7 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 11:30 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3584 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1823 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93093 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 10:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 70; 70 X 70MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; EOSINOPHILIC CELL,-MINIMAL WEEK 89; 45 X 45MM; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); AXILLARY REGION-LEFT; WEEK MINIMAL 91; 10 X 10MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MODERATE OBSERVED -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK >NOTE:>SINGLE SMALL AREA OF HEMORRHAGE 94; 15 X 15MM; VERIFIED AT NECROPSY: WITH PROTEINACEOUS-APPEARING OBSERVED LAMELLAR SPHERULES. >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK SPLEEN (SP) : 94; 10 X 10MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY, OBSERVED INCREASED,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -LARGE; LEFT: 10 MM IN DIAMETER (RIGHT -ANGIECTASIS,-SLIGHT >EXCRETION; FEW FECES; VERIFIED AT IS 5 MM IN DIAMETER) -HEMORRHAGE,-PRESENT NECROPSY: NOT APPLICABLE -CYST(S); LEFT: SINGLE CLEAR FLUID -HYPERTROPHY, CORTICAL CELL,-MODERATE >APPEARANCE; THIN; VERIFIED AT FILLED CYST, 6 MM IN DIAMETER -DEGENERATION, CYSTIC / HEMORRHAGIC,- NECROPSY: OBSERVED MINIMAL -PIGMENT,-SLIGHT 3585 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1824 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93093 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 10:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 13 X 8 -B-ADENOMA,-NEOPLASM PRESENT MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 90 X 65 X 30 MM, RED, -CYST / GALACTOCELE,-PRESENT TAN, VASCULAR, AND SOFT; SOME AREAS -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT EXUDE A TAN CREAMY SEMISOLID; PORTION -HYPERPLASIA, GLANDULAR,-MODERATE COLLECTED; REGIONAL LN (INGUINAL) -B-ADENOMA,-NEOPLASM PRESENT NORMAL (CAP 21) >NOTE:>MASSES 1 & 5 ARE FIBROADENOMAS -MASS #2; RIGHT INGUINAL REGION: MASSES 3 & 4 ARE CARCINOMAS MASS SINGLE MASS, 40 X 40 X 20 MM, DARK 2 IS AN ADENOMA RED, DARK BROWN, AND SOFT; ON SECTION EXUDES A CLEAR DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) 3586 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1825 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93093 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 10:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 25 X 15 X 10 MM, DARK BROWN, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #4; LEFT CERVICAL REGION: SINGLE MASS, 25 X 20 X 15 MM, TAN, FIRM AND VASCULAR; ON SECTION EXUDES A TAN AND GREEN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) -MASS #5; RIGHT CERVICAL REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND SOFT; ON SECTION EXUDES A YELLOW AND TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MINIMAL 3587 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1826 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93093 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 10:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -------------------------------------------- 3588 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1827 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93093 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 10:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3589 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1828 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93094 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 7:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK EYE (EY) : 76; 55 X 60MM; VERIFIED AT NECROPSY: -INFLAMMATION, ACUTE,-SLIGHT OBSERVED BRAIN (BR) : BRAIN (BR) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT CERVICAL-RIGHT; VERIFIED AT NECROPSY: LIVER (LI) : LIVER (LI) : OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN -HYPERTROPHY, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: NOT APPLICABLE SURFACE OF RIGHT MEDIAN LOBE: SINGLE OCCASIONALLY NOTED,-SLIGHT >EYES; RED DISCHARGE; EYES; VERIFIED AT RED FOCUS, 3 MM IN DIAMETER; COLLECTED -VACUOLATION, HEPATOCELLULAR, NECROPSY: OBSERVED ON ROUTINE SECTION PERIPORTAL,-MODERATE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / VERIFIED AT NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE 3590 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1829 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93094 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 7:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT CERVICAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 60 X 40 X 30 MM, RED, -CYST / GALACTOCELE,-PRESENT DARK RED, TAN, LOBULATED, VASCULAR, -HYPERPLASIA, GLANDULAR,-SLIGHT AND FIRM; SOME AREAS EXUDE A DARK BROWN SEMISOLID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21); OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN, DRY CRUSTED AREA, 12, X 10 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3591 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1830 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93094 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 7:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 3592 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1831 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93094 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 7:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3593 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1832 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93095 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/00 16:05 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 79; 40 X 35MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; AXILLARY -VACUOLATION, HEPATOCELLULAR, MIDZONAL REGION-LEFT; VERIFIED AT NECROPSY: / CENTRILOBULAR,-MODERATELY SEVERE OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; MINIMAL 20 X 20MM; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >MASS(ES); INGUINAL REGION-LEFT; WEEK SLIGHT 97; 55 X 40MM; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-SLIGHT OBSERVED SPLEEN (SP) : >EYES; PALE; EYES; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE INCREASED,-SEVERE >SKIN & PELAGE; PALE; ENTIRE BODY; ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; -HYPERTROPHY, CORTICAL CELL,-MODERATE VERIFIED AT NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC / HEMORRHAGIC,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT SLIGHT NECROPSY: NOT APPLICABLE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -CYST,-PRESENT 3594 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1833 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93095 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/00 16:05 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; DUE TO -DEPLETION, LYMPHOCYTIC (GENERALLY ADHESIONS, POSSIBLE REMNANTS SUBMITTED COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SEVERE LUNG (LU) : LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE TAN, -N-CARCINOMA,-FROM MAMMARY, FEMALE RED AND FIRM MASSES, UP TO 13 X 7 X 4 MM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED -ADHESION(S); ALL LOBES ADHERED TO EACH OTHER, DIAPHRAGM, HEART AND THYMUS: MULTIPLE GREY FIBROUS ADHESIONS; COLLECTED INTACT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED , DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND FIRM STOMACH, GL (ST) : >UNREMARKABLE 3595 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1834 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93095 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/00 16:05 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER SKIN (SK) : >NOTE:>SKIN WAS TAKEN FROM RIGHT SIDE DUE TO MASS ON LEFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 38 X 25 X 15 MM, RED, -CYST / GALACTOCELE,-PRESENT TAN, LOBULATED AND FIRM; ON SECTION, -B-FIBROADENOMA,-NEOPLASM PRESENT EXUDED A TAN FLUID; COLLECTED; >NOTE:>MASS #1 IS THE CARCINOMA REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A DARK RED AND DRY CRUSTED AREA, 35 X 25 MM; COLLECTED INTACT WITH MASS -MASS #2; LEFT INGUINAL EXTENDING INTO VENTRAL ABDOMINAL: SINGLE MASS, 65 X 50 X 31 MM, RED, DARK RED, TAN, LOBULATED AND FIRM: SOME SECTIONS EXUDE A RED FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>MAMMARY WAS TAKEN FROM RIGHT SIDE DUE TO MASS ON LEFT UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: >UNREMARKABLE MULTIPLE CLEAR FLUID FILLED CYSTS, 2 MM IN DIAMETER 3596 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1835 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93095 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/00 16:05 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT LIGHT GREEN MUCOID MATERIAL -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 12 X 10 X 3 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,- (RIGHT NOT IDENTIFIED) (CAP 21) MODERATELY SEVERE GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; CLEAR FLUID -------------------------------------------- 3597 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1836 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93095 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/00 16:05 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3598 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1837 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93096 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/98 STUDY DAY OF DEATH: 217 STUDY WEEK OF DEATH: 31 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/98 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT LUNG (LU) : -CONGESTION,-PRESENT -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -MINERALIZATION, VASCULAR,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -------------------------------------------- 3599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1838 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93096 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/98 STUDY DAY OF DEATH: 217 STUDY WEEK OF DEATH: 31 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/98 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1839 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93097 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 8:33 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 63; 40 X 35MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT 69; 55 X 60MM; VERIFIED AT NECROPSY: -NECROSIS, INDIVIDUAL HEPATOCYTE,- OBSERVED MINIMAL >MASS(ES); VENTRAL-CERVICAL; WEEK 78; -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / 50 X 50MM; VERIFIED AT NECROPSY: EOSINOPHILIC CELL,-MINIMAL OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- >SKIN & PELAGE; SORE/SCAB; VENTRAL- MINIMAL THORACIC-LEFT; VERIFIED AT NECROPSY: KIDNEY (KD) : OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; BLACK SKIN; VENTRAL- MINIMAL THORACIC-LEFT; VERIFIED AT NECROPSY: SPLEEN (SP) : NOT APPLICABLE -HEMATOPOIESIS, EXTRAMEDULLARY, >DISCHARGE; OTHER; AXILLARY LEFT MASS INCREASED,-MODERATE BLEEDING; VERIFIED AT NECROPSY: ADRENAL, CORTEX (AC) : OBSERVED -VACUOLATION, FOCAL,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -HYPERTROPHY, CORTICAL CELL,-SLIGHT NECROPSY: NOT APPLICABLE -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE 3601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1840 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93097 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 8:33 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND FIRM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT CERVICAL REGION, -M-CARCINOMA,-NEOPLASM PRESENT EXTENDING INTO RIGHT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 55 X 50 X 22 MM, FIRM, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT LOBULATED, RED, AND TAN; ON SECTION -HYPERPLASIA, GLANDULAR,-SLIGHT SOME AREAS CONTAIN A TAN CREAMY >NOTE:>THE CARCINOMA IS MASS #3 SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 47 X 36 X 23 MM, FIRM, LOBULATED, RED, AND TAN; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1841 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93097 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 8:33 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 62 X 56 X 50 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED, ON SECTION, SOME AREAS CONTAIN A BROWN FLUID; COLLECTED; OVERLYING SKIN HAS A SINGLE DRY, RED, BROWN ULCERATED AREA, 30 X 45 MM, WHICH EXUDES A DARK RED FLUID; COLLECTED WITH MASS; REGIONAL LN (SEE AXILLARY) CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 15 X 8 X 3 -HYPERPLASIA, LYMPHOPLASMACYTIC,- MM, RIGHT IS 9 X 5 X 2 MM (CAP 21) MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1842 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93097 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 8:33 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1843 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93098 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 469.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:08 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 100; VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1844 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93098 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 469.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:08 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -HYPERPLASIA, GLANDULAR,-MINIMAL SINGLE MASS, 10 X 9 X 6 MM, RED, TAN, -M-FIBROSARCOMA,-NEOPLASM PRESENT AND FIRM; SAME ON CUT SURFACE (CAP 22) REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -MASS(ES); RIGHT HORN WITHIN WALL: -B-ADENOMA,-NEOPLASM PRESENT MULTIPLE DARK RED AND TAN PEDUNCULATED MASSES, EACH IS UP TO 5 X 4 X 3 MM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1845 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93098 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 469.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:08 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1846 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93099 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 7:39 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 79; 30 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -MINERALIZATION,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK LIVER (LI) : 72; 20 X 20MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, >APPEARANCE; HEAD TILT; LEFT; VERIFIED PERIPORTAL,-SLIGHT AT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >BEHAVIOR; HYPOACTIVE; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT >EXCRETION; NO FECES; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >DISCHARGE; GENITAL; RED IN COLOR; MINIMAL VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -ATROPHY, CORTICAL,-SLIGHT 3608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1847 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93099 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 7:39 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 14 X 11 X 6 -B-ADENOMA,-NEOPLASM PRESENT MM, RED, DARK RED, TAN, SOFT, AND LOBULATED MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 25 X 25 X 16 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT TAN, AND FIRM; SAME ON CUT SURFACE; -HYPERPLASIA, GLANDULAR,-SLIGHT COLLECTED; REGIONAL LN (INGUINAL) NOT -B-ADENOMA,-NEOPLASM PRESENT IDENTIFIED >NOTE:>THE ADENOMA IS MASS #2 -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND SOFT; ON SECTION EXUDES A CLEAR FLUID; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,- NEOPLASM PRESENT 3609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1848 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93099 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 7:39 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF RIGHT HORN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SINGLE MASS, 16 X 6 X 6 MM, DARK RED, MODERATELY SEVERE SOFT, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT WITH UTERUS; REGIONAL LN (LUMBAR) NORMAL (CAP 21) VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK RED AND DRY -------------------------------------------- 3610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1849 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93099 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 7:39 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1850 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93100 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:10 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1851 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93100 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:10 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 6 X 4 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, TAN, GRAY AND SOFT DUODENUM (DU) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT (CAP 21) -------------------------------------------- 3613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1852 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93100 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:10 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1853 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93101 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:52 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 X 4 X 3 MM (LEFT IS 5 -HYPERTROPHY, CORTICAL CELL,-MINIMAL X 3 X 3 MM) -PIGMENT,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1854 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93101 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:52 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), ADRENAL, MEDULLA (MA), LUNG (LU), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1855 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93102 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 519.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL 3617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1856 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93102 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 519.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 X 5 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, AND SOFT -CYST,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : UTERUS (UT) : -CYST(S); RIGHT WITHIN WALL: SINGLE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- CLEAR FLUID FILLED CYST, 3 MM IN MINIMAL DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3618 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1857 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93102 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 519.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3619 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1858 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93103 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >SKIN & PELAGE; SORE/SCAB; PAW-HIND- -INFILTRATE, MACROPHAGE, PIGMENTED,- RIGHT; VERIFIED AT NECROPSY: OBSERVED MINIMAL >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT >APPEARANCE; MISSING; TEETH; VERIFIED -VACUOLATION, HEPATOCELLULAR, AT NECROPSY: OBSERVED PERIPORTAL,-SEVERE >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, NECROPSY: NOT APPLICABLE BASOPHILIC,-MINIMAL >EXCRETION; NO FECES; VERIFIED AT -HYPERPLASIA, BILE DUCT,-SLIGHT NECROPSY: NOT APPLICABLE -FIBROSIS, PERIBILIARY,-SLIGHT >SKIN & PELAGE; BROWN HAIR COAT; -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL VENTRAL-ABDOMINAL; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT 3620 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1859 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93103 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 2 MM IN DIAMETER BONE, FEMUR (FE) : BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: FEMUR NOT >TISSUE MISSING FOUND AT TIME OF TRIMMING MARROW, FEMUR (FM) : MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: FEMUR NOT >TISSUE MISSING FOUND AT TIME OF TRIMMING THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE -ACANTHOSIS / HYPERKERATOSIS,- TAN ULCERATED AREA, 12 X 7 MM MODERATELY SEVERE -THICKENED WALL; DIFFUSE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -FIBROSIS,-MODERATELY SEVERE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; -CYST / GALACTOCELE,-PRESENT COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-SLIGHT 3621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1860 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93103 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>NEOPLASM IS ON SLIDE #19 UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE HINDFEET: SINGLE MOIST TAN ULCERATED -ULCERATION,-SLIGHT AREA ON EACH, EACH IS 6 MM IN -ACANTHOSIS / HYPERKERATOSIS,-MODERATE DIAMETER; BOTH ARE COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER LEFT INCISOR: MISSING -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL: MOIST BROWN MATERIAL -------------------------------------------- 3622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1861 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93103 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1862 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93104 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:45 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 58; 45 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -INFILTRATE, MACROPHAGE, PIGMENTED,- OBSERVED MINIMAL >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HYPERTROPHY, HEPATOCELLULAR, THORACIC-LEFT; VERIFIED AT NECROPSY: CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1863 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93104 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:45 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 12 X 9 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL -ACANTHOSIS/HYPERKERATOSIS,-MODERATE THORACIC REGION: TWO TAN, RED AND DRY -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SLIGHTLY RAISED AREAS, ONE IS 7 X 6 X -ULCERATION,-MODERATE 3 MM, THE OTHER IS 8 X 6 X 5 MM (CAP >NOTE:>SEE ALSO MAMMARY GLAND 22) >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MASS MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1864 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93104 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:45 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: >NOTE:>THE MASS CONTAINS BOTH CARCINOMA MULTIPLE MASSES, UP TO 30 X 30 X 15 AND FIBROADENOMA THE SECOND MM, SOME ARE TAN AND SOFT; SAME ON CUT MAMMARY CARCINOMA WAS IN THE SURFACE; SOME ARE DARK RED, TAN AND ULCERATED SKIN SECTION SOFT; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MASS CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 10 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 7 X 3 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1865 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93104 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:45 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1866 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93105 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:26 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; SWOLLEN; LIMB-HIND-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- IS 5 MM IN DIAMETER) SEVERE LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK BROWN >UNREMARKABLE 3628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1867 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93105 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:26 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 9 X 9 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, GREY, TAN AND SOFT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM (COLLECTED ON ROUTINE -HYPERPLASIA, GLANDULAR,-MODERATE SECTION) UTERUS (UT) : -DILATATION,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SINGLE TAN AND DRY CRUSTED AREA, 4 X 3 -ULCERATION,-SLIGHT MM; SURROUNDING TISSUE APPEARS SWOLLEN -ACANTHOSIS / HYPERKERATOSIS,-MODERATE (COLLECTED) 3629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1868 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93105 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:26 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1869 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93106 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/09/00 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 02/09/00 11:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MISSING; TAIL-DISTAL; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); AT JUNCTION -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE OF MEDIAN LOBES: SINGLE TAN AREA, 4 MM -VACUOLATION, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; VERIFIED AT IN DIAMETER (CAP 21) PERIPORTAL,-MODERATE NECROPSY: NOT APPLICABLE -RED FOCUS(I)/AREA(S); NEAR JUNCTION -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / OF MEDIAN LOBES: MULTIPLE DARK RED EOSINOPHILIC CELL,-MINIMAL AREAS, UP TO 4 MM IN DIAMETER (CAP 21) -FOCUS, ALTERED HEPATOCELLULAR, -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC BASOPHILIC,-MINIMAL SURFACE OF LEFT LATERAL LOBE: SINGLE -DEGENERATION, CYSTIC,-MINIMAL GRAY, LINEAR AREA, 4 X 2 MM; COLLECTED -INFILTRATE, MACROPHAGE, PIGMENTED,- ON ROUTINE SECTION MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT 3631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1870 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93106 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/09/00 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 02/09/00 11:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY AND SOFT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -HYPERPLASIA, MUCINOUS,-MODERATE SMALL AMOUNT OF LIGHT GREEN SEMIFLUID CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; TIP: MISSING; REMAINING PORTION COLLECTED -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 3632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1871 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93106 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/09/00 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 02/09/00 11:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1872 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93107 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:20 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; MISSING; TEETH; VERIFIED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / AT NECROPSY: NOT OBSERVED EOSINOPHILIC CELL,-MINIMAL >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK -FOCUS, ALTERED HEPATOCELLULAR, 81; 70 X 80MM; VERIFIED AT NECROPSY: BASOPHILIC,-MINIMAL OBSERVED -NECROSIS, COAGULATIVE,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR -HYPERPLASIA,-MODERATELY SEVERE ASPECT: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL CERVICAL -B-FIBROADENOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 80 X 60 X 40 MM, -HYPERPLASIA, GLANDULAR,-MODERATE TAN, RED, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE 3634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1873 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93107 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:20 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR, BROKEN AT BASE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1874 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93108 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/18/99 STUDY DAY OF DEATH: 364 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/18/99 14:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE RIGHT -HEMORRHAGE,-PRESENT ADRENAL, SURROUNDING RIGHT KIDNEY: SINGLE MASS, 20 MM IN DIAMETER, DARK RED AND SOFT; CUT SURFACE EXUDES A DARK RED FLUID; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 3636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1875 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93108 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/18/99 STUDY DAY OF DEATH: 364 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/18/99 14:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -HEMORRHAGE,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1876 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93109 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 259.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0478 .0184 % .0236 WEIGHT TAKEN RT ADRENAL (AD1) .0379 .0146 % .0187 WEIGHT TAKEN BRAIN (BR) 2.0285 .7829 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1768 .4542 % .5801 WEIGHT TAKEN RT KIDNEY (KD1) 1.2596 .4861 % .6210 WEIGHT TAKEN LIVER (LI) 10.0793 3.8901 % 4.9688 WEIGHT TAKEN LUNG (LU) 1.3962 .5389 % .6883 WEIGHT TAKEN LF OVARY (OV0) .0846 .0327 % .0417 WEIGHT TAKEN RT OVARY (OV1) .1074 .0415 % .0529 WEIGHT TAKEN SPLEEN (SP) .5017 .1936 % .2473 WEIGHT TAKEN LF THYROID/PARA (TT0) .0087 .0034 % .0043 WEIGHT TAKEN RT THYROID/PARA (TT1) .0101 .0039 % .0050 WEIGHT TAKEN UTERUS (UT) .8250 .3184 % .4067 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1877 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93109 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 259.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1878 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93110 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:45 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK EYE (EY) : 90; 20 X 20MM; VERIFIED AT NECROPSY: -DYSTROPHY, CORNEAL IN EARLY STAGES, OBSERVED CONSISTS PRIMARILY OF FOCAL >APPEARANCE; THIN; VERIFIED AT MINERALIZATION,-MINIMAL NECROPSY: OBSERVED -INFLAMMATION, ACUTE,-SLIGHT >SKIN & PELAGE; ROUGH HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -MOTTLED; ALL LOBES: DARK RED AND TAN -VACUOLATION, HEPATOCELLULAR, >EYES; RED DISCHARGE; EYE-LEFT; PERIPORTAL,-SEVERE VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >EXCRETION; NONFORMED FECES; VERIFIED EOSINOPHILIC CELL,-MINIMAL AT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT BASOPHILIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN -NEPHROPATHY, CHRONIC PROGRESSIVE,- PELVIS: MULTIPLE TAN GRANULES, 1 MM IN SEVERE DIAMETER -MINERALIZATION, PELVIC,-SLIGHT -ROUGH SURFACE, DIFFUSE; BILATERAL -MOTTLED; BILATERAL: DARK RED AND TAN SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 3640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1879 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93110 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:45 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT SALIV GL, MANDIB (SG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK BROWN FOCI, UP TO 2 MM IN DIAMETER DUODENUM (DU) : -INFLAMMATION, VASCULAR,-SLIGHT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 30 X 20 X 10 MM, TAN AND >NOTE:>SEE SKIN FOR DIAGNOSIS FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) 3641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1880 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93110 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:45 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN VAGINA: -LUMEN, EXUDATE,-PRESENT LARGE AMOUNT OF GREEN SEMIFLUID CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK RED AND TAN -HYPERPLASIA, LYMPHOPLASMACYTIC,- (CAP 21) MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1881 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93110 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:45 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1882 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93111 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:01 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0325 .0113 % .0143 WEIGHT TAKEN RT ADRENAL (AD1) .0326 .0113 % .0144 WEIGHT TAKEN BRAIN (BR) 2.2661 .7882 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1506 .4002 % .5077 WEIGHT TAKEN RT KIDNEY (KD1) 1.1943 .4154 % .5270 WEIGHT TAKEN LIVER (LI) 10.8555 3.7758 % 4.7904 WEIGHT TAKEN LUNG (LU) 1.4316 .4979 % .6317 WEIGHT TAKEN LF OVARY (OV0) .0693 .0241 % .0306 WEIGHT TAKEN RT OVARY (OV1) .0788 .0274 % .0348 WEIGHT TAKEN SPLEEN (SP) .4755 .1654 % .2098 WEIGHT TAKEN LF THYROID/PARA (TT0) .0096 .0033 % .0042 WEIGHT TAKEN RT THYROID/PARA (TT1) .0113 .0039 % .0050 WEIGHT TAKEN UTERUS (UT) .4887 .1700 % .2157 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 3644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1883 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93111 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:01 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1884 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93112 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0342 .0100 % .0153 WEIGHT TAKEN RT ADRENAL (AD1) .0328 .0096 % .0147 WEIGHT TAKEN BRAIN (BR) 2.2296 .6525 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.5592 .4563 % .6993 WEIGHT TAKEN RT KIDNEY (KD1) 1.6358 .4787 % .7337 WEIGHT TAKEN LIVER (LI) 16.7616 4.9054 % 7.5178 WEIGHT TAKEN LUNG (LU) 1.5802 .4625 % .7087 WEIGHT TAKEN LF OVARY (OV0) .0394 .0115 % .0177 WEIGHT TAKEN RT OVARY (OV1) .0495 .0145 % .0222 WEIGHT TAKEN SPLEEN (SP) .4772 .1397 % .2140 WEIGHT TAKEN LF THYROID/PARA (TT0) .0095 .0028 % .0043 WEIGHT TAKEN RT THYROID/PARA (TT1) .0089 .0026 % .0040 WEIGHT TAKEN UTERUS (UT) .8851 .2590 % .3970 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL 3646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1885 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93112 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -HEMORRHAGE,-PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); MID ASPECT: SINGLE -B-ADENOMA,-NEOPLASM PRESENT RED AREA, 2 MM IN DIAMETER -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1886 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93112 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1887 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93113 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:50 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 103; 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 6 MM IN -HYPERTROPHY, HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE CENTRILOBULAR POLYKARYOCYTOSIS SECTIONS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1888 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93113 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:50 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -MOTTLED; BILATERAL: TAN AND DARK -HYPERTROPHY, CORTICAL CELL,-MODERATELY BROWN SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 5 MM IN DIAMETER -HYPERPLASIA, GRANULOSA CELL,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 7 X 5 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT 3650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1889 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93113 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:50 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -HYPERPLASIA,-SLIGHT -CYST,-PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM; COLLECTED ON ROUTINE -B-FIBROADENOMA,-NEOPLASM PRESENT SECTION; POSSIBLY CONSISTENT WITH MASS -HYPERPLASIA, GLANDULAR,-SLIGHT (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATIONS -CYST(S); LEFT INGUINAL REGION: SINGLE TAN FLUID FILLED CYST, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 3651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1890 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93113 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:50 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1891 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93114 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:20 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.8332 3.8144 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1892 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93114 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:20 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1893 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93115 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE TAN AREAS, UP TO 3 MM IN -HYPERTROPHY, HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE CENTRILOBULAR POLYKARYOCYTOSIS SECTIONS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL 3655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1894 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93115 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 7 X 6 X 5 -ANGIECTASIS,-MODERATE MM, RIGHT IS 5 X 5 X 4 MM -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -LARGE; LEFT: 10 X 6 X 4 MM (RIGHT IS -HYPERPLASIA, GRANULOSA CELL,- 6 X 5 X 3 MM) MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT 3656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1895 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93115 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM; COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1896 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93115 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1897 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93116 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 288.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:22 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0353 .0122 % .0165 WEIGHT TAKEN RT ADRENAL (AD1) .0364 .0126 % .0171 WEIGHT TAKEN BRAIN (BR) 2.1339 .7386 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.4382 .4978 % .6740 WEIGHT TAKEN RT KIDNEY (KD1) 1.4407 .4987 % .6751 WEIGHT TAKEN LIVER (LI) 13.8981 4.8107 % 6.5130 WEIGHT TAKEN LUNG (LU) 1.4611 .5057 % .6847 WEIGHT TAKEN LF OVARY (OV0) .0612 .0212 % .0287 WEIGHT TAKEN RT OVARY (OV1) .0620 .0215 % .0291 WEIGHT TAKEN SPLEEN (SP) .4803 .1663 % .2251 WEIGHT TAKEN LF THYROID/PARA (TT0) .0085 .0029 % .0040 WEIGHT TAKEN RT THYROID/PARA (TT1) .0104 .0036 % .0049 WEIGHT TAKEN UTERUS (UT) 1.2623 .4369 % .5915 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1898 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93116 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 288.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:22 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT URINARY BLADDER (UB) : URINARY BLADDER (UB) : >NOTE:>TRIMMING COMMENT: URINARY >TISSUE MISSING BLADDER NOT FOUND AT TIME OF TRIMMING VAGINA (VA) : VAGINA (VA) : >NOTE:>TRIMMING COMMENT: VAGINA NOT >TISSUE MISSING FOUND AT TIME OF TRIMMING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1899 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93116 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 288.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:22 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1900 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93117 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0325 .0083 % .0143 WEIGHT TAKEN RT ADRENAL (AD1) .0369 .0094 % .0162 WEIGHT TAKEN BRAIN (BR) 2.2739 .5817 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.5504 .3966 % .6818 WEIGHT TAKEN RT KIDNEY (KD1) 1.4994 .3836 % .6594 WEIGHT TAKEN LIVER (LI) 16.3241 4.1760 % 7.1789 WEIGHT TAKEN LUNG (LU) 1.6859 .4313 % .7414 WEIGHT TAKEN LF OVARY (OV0) .0392 .0100 % .0172 WEIGHT TAKEN RT OVARY (OV1) .0484 .0124 % .0213 WEIGHT TAKEN SPLEEN (SP) .7833 .2004 % .3445 WEIGHT TAKEN LF THYROID/PARA (TT0) .0150 .0038 % .0066 WEIGHT TAKEN RT THYROID/PARA (TT1) .0061 .0016 % .0027 WEIGHT TAKEN UTERUS (UT) 1.0597 .2711 % .4660 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1901 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93117 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN -HYPERPLASIA,-MODERATELY SEVERE -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1902 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93117 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1903 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93118 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 49; 90 X 90MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >APPEARANCE; MALOCCLUSION; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,- NECROPSY: NOT OBSERVED MODERATE >APPEARANCE; THIN; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS >APPEARANCE; MISSING; TEETH; VERIFIED OCCASIONALLY NOTED,-SLIGHT AT NECROPSY: OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, >SKIN & PELAGE; SORE/SCAB; VENTRAL- CENTRILOBULAR,-SLIGHT ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -PIGMENT, HEPATOCELLULAR, OBSERVED CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -INFLAMMATION, SUBACUTE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 60 X 15 X 10 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE 3665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1904 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93118 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -HYPERPLASIA, CORTICAL,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); TRIMMING -INFILTRATE, MACROPHAGE, ALVEOLAR,- COMMENT: ALL LOBES: MULTIPLE TAN SLIGHT AREAS, UP TO 4 X 3 MM HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 6 X 4 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 3666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1905 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93118 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION EXTENDING LATERALLY TO LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 60 X 30 X 25 MM, ALL ARE RED, TAN, GREEN, LOBULATED AND FIRM; SAME ON ALL CUT SURFACES, ALL COLLECTED INTACT; OVERLYING SKIN HAS A SINGLE MOIST RED ULCERATED AREA, 10 MM IN DIAMETER; COLLECTED INTACT WITH MASSES; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 9 X 6 X 3 -HYPERPLASIA, LYMPHOPLASMACYTIC,- MM; COLLECTED MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL UPPER INCISORS -ANIMAL THIN -------------------------------------------- 3667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1906 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93118 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 13:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1907 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93119 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 15:17 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -INFLAMMATION, ACUTE,-SLIGHT >SKIN & PELAGE; SORE/SCAB; SHOULDER- BRAIN (BR) : BRAIN (BR) : RIGHT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; DORSAL; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT -HYPERTROPHY, CORTICAL CELL,-MODERATE IS 4 MM IN DIAMETER) -DEGENERATION, CYSTIC / HEMORRHAGIC,- SEVERE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 3669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1908 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93119 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 15:17 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -MINERALIZATION, VASCULAR,-MINIMAL MULTIPLE TAN AREAS, UP TO 10 MM IN -INFILTRATE, MACROPHAGE, ALVEOLAR,- DIAMETER MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : SKIN (SK) : -ABRASION(S)/ULCERATION(S); RIGHT -INFLAMMATION, CHRONIC,-MODERATE SHOULDER REGION: MULTIPLE DRY BROWN ABRADED AREAS, UP TO 2 MM IN DIAMETER (CAP 22) -CRUSTED AREA(S); MID DORSAL CERVICAL REGION: MULTIPLE DRY BROWN CRUSTED AREAS, UP TO 2 MM IN DIAMETER (CAP 21) MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT GREEN MUCOID MATERIAL -HYPERPLASIA, MUCINOUS,-MODERATE 3670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1909 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93119 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 15:17 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -HYPERPLASIA, LYMPHOPLASMACYTIC,- -LARGE; BILATERAL: EACH IS 10 X 5 X 3 MODERATELY SEVERE MM; COLLECTED -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1910 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93119 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 15:17 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1911 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93120 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 285.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0343 .0120 % .0164 WEIGHT TAKEN RT ADRENAL (AD1) .0334 .0117 % .0160 WEIGHT TAKEN BRAIN (BR) 2.0929 .7336 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2810 .4490 % .6121 WEIGHT TAKEN RT KIDNEY (KD1) 1.2307 .4314 % .5880 WEIGHT TAKEN LIVER (LI) 12.5676 4.4050 % 6.0049 WEIGHT TAKEN LUNG (LU) 1.6572 .5809 % .7918 WEIGHT TAKEN LF OVARY (OV0) .0448 .0157 % .0214 WEIGHT TAKEN RT OVARY (OV1) .0377 .0132 % .0180 WEIGHT TAKEN SPLEEN (SP) .4264 .1495 % .2037 WEIGHT TAKEN LF THYROID/PARA (TT0) .0083 .0029 % .0040 WEIGHT TAKEN RT THYROID/PARA (TT1) .0103 .0036 % .0049 WEIGHT TAKEN UTERUS (UT) 1.3539 .4746 % .6469 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1912 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93120 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 285.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 3 X 2 MM SLIGHT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -HYPERPLASIA,-MODERATE SINGLE DARK RED FOCUS, 1 MM IN -ANGIECTASIS,-PRESENT DIAMETER MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1913 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93120 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 285.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1914 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93121 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 158.0 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 7:40 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >APPEARANCE; HEAD TILT; LEFT; VERIFIED -HYPERTROPHY, HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >BEHAVIOR; HYPOACTIVE; VERIFIED AT OCCASIONALLY NOTED,-SLIGHT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >APPEARANCE; TREMORS; ENTIRE BODY; EOSINOPHILIC CELL,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, STERNUM (SE) : MARROW, STERNUM (SE) : >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE 3676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1915 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93121 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 158.0 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 7:40 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ BONE, STERNUM (SB) : BONE, STERNUM (SB) : >NOTE:>TRIMMING COMMENT: MISSING FROM >TISSUE MISSING WET TISSUE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL MUSCLE, SKELETAL (SM) : MUSCLE, SKELETAL (SM) : -ATROPHY-DIFFUSE; COLLECTED ON ROUTINE -ATROPHY,-MINIMAL SECTION THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, SOFT AND LOBULATED STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -EROSION,-MINIMAL MULTIPLE DARK BROWN AREAS, UP TO 8 X 2 MM RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1916 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93121 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 158.0 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 7:40 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; JEJUNUM: DARK GREEN SEMIFLUID -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1917 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 67; 60 X 40MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -HYPERTROPHY, HEPATOCELLULAR, 71; 40 X 40MM; VERIFIED AT NECROPSY: CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED OCCASIONALLY NOTED,-SLIGHT >MASS(ES); INGUINAL REGION-RIGHT; WEEK -GRANULAR CYTOPLASM, EOSINOPHILIC, 72; 20 X 15MM; VERIFIED AT NECROPSY: CENTRILOBULAR,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >MASS(ES); INGUINAL REGION-RIGHT; WEEK BASOPHILIC,-MINIMAL 78; 20 X 20MM ANTERIOR TO OTHER MASS; KIDNEY (KD) : KIDNEY (KD) : VERIFIED AT NECROPSY: OBSERVED >NOTE:>TRIMMING COMMENT: BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- >EXCRETION; FEW FECES; VERIFIED AT MODERATELY SEVERE NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MODERATE >EXCRETION; DISCOLORED FECES; YELLOW IN -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT COLOR; VERIFIED AT NECROPSY: NOT PANCREAS (PA) : APPLICABLE -ZYMOGEN GRANULES, DECREASED,-SEVERE >APPEARANCE; THIN; VERIFIED AT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : NECROPSY: OBSERVED -LARGE; RIGHT: 17 X 15 X 12 MM (LEFT -HEMATOPOIESIS, EXTRAMEDULLARY,- >BEHAVIOR; HYPOACTIVE; VERIFIED AT IS 4 X 2 X 2 MM) MODERATE NECROPSY: NOT APPLICABLE -DEGENERATION, CYSTIC / HEMORRHAGIC,- SEVERE -PIGMENT,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT 3679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1918 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION / NECROSIS, UNILATERAL,- SEVERE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT FIRM STOMACH, GL (ST) : -EROSION,-MINIMAL 3680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1919 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN LINEAR AREAS, UP TO 5 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL: SINGLE -M-CARCINOMA,-NEOPLASM PRESENT MASS, 60 X 40 X 32 MM, RED, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT LOBULATED AND FIRM; SAME ON CUT >NOTE:>MASS #1 IS PRIMARILY A SURFACE; COLLECTED INTACT; REGIONAL LN CARCINOMA, HOWEVER, THERE ARE (INGUINAL) NOT IDENTIFIED; POSSIBLY ALSO AREAS OF FIBROADENOMA CONSISTENT WITH ALL MASSES (RIGHT INGUINAL) NOTED IN CLINICAL OBSERVATION -MASS #2; LEFT INGUINAL: SINGLE MASS, 55 X 32 X 22 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATION UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE -DILATATION,-SEVERE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1920 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1921 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93123 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:25 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.0297 4.0391 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1922 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93123 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:25 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1923 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93124 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 72; 70 X 60 MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HYPERTROPHY, HEPATOCELLULAR, ABDOMINAL-LEFT; VERIFIED AT NECROPSY: CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED OCCASIONALLY NOTED,-SLIGHT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -GRANULAR CYTOPLASM, EOSINOPHILIC, WEEK 94; 30 X 30 MM; VERIFIED AT CENTRILOBULAR,-SLIGHT NECROPSY: OBSERVED -PIGMENT, HEPATOCELLULAR, >MASS(ES); SHOULDER-LEFT; WEEK 95; 10 X CENTRILOBULAR,-MODERATE 10MM; VERIFIED AT NECROPSY: OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BASOPHILIC,-MINIMAL 71; 30 X 40 MM; VERIFIED AT NECROPSY: -NECROSIS, COAGULATIVE,-MINIMAL OBSERVED LIVER-OIL RED O (LI0) : >APPEARANCE; THIN; VERIFIED AT -VACUOLES, OIL RED O-POSITIVE, NECROPSY: OBSERVED HEPATOCELLULAR,-MINIMAL >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK KIDNEY (KD) : 97; 20 X 15MM; VERIFIED AT NECROPSY: -NEPHROPATHY, CHRONIC PROGRESSIVE,- OBSERVED MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK -MINERALIZATION, PELVIC,-MINIMAL 102; 10 X 10MM; VERIFIED AT NECROPSY: SPLEEN (SP) : OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE 3685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1924 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93124 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK OVARY (OV) : OVARY (OV) : 102; 10 X 10MM; VERIFIED AT NECROPSY: -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT OBSERVED FLUID FILLED CYST ON EACH, LEFT IS 4 >SKIN & PELAGE; BLUE SKIN; VENTRAL- MM IN DIAMETER, RIGHT IS 3 MM IN ABDOMINAL-LEFT; VERIFIED AT NECROPSY: DIAMETER NOT APPLICABLE MARROW, FEMUR (FM) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -HYPERPLASIA, MYELOID, MARROW,- ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: MODERATELY SEVERE OBSERVED MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 4 X 3 MM MODERATE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED, TAN AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 6 NEOPLASMS PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT >NOTE:>MASSES 6 AND 7 ARE THE FIBROADENOMAS 3686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1925 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93124 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 23 X 16 X 11 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #2; LEFT AXILLARY REGION, CAUDAL TO MASS #1: SINGLE MASS, 20 X 19 X 12 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 44 X 34 X 25 MM, TAN, RED, LOBULATED, VASCULAR AND FIRM; ON SECTION EXUDES A BROWN SEMIFLUID (CAP 28); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 31 X 15 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (AXILLARY) NORMAL (CAP 24); OVERLYING SKIN HAS A MOIST RED ULCERATED AREA, 17 X 15 MM; COLLECTED INTACT WITH MASS -MASS #5; RIGHT AXILLARY REGION, CAUDAL TO MASS #4: SINGLE MASS, 34 X 25 X 15 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 24) 3687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1926 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93124 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #6; RIGHT INGUINAL REGION: SINGLE MASS, 75 X 65 X 34 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE MOIST, RED ULCERATED AREA, 20 X 11 MM; COLLECTED INTACT WITH MASS -MASS #7; RIGHT INGUINAL REGION, CAUDAL TO MASS #6: SINGLE MASS, 21 X 15 X 12 MM; RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #8; RIGHT PERIANAL REGION: SINGLE MASS, 21 X 13 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 3688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1927 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93124 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1928 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93125 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:40 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN -GRANULAR CYTOPLASM, EOSINOPHILIC, -LIGHT FOCUS(I)/AREA(S); ALL LOBES: CENTRILOBULAR,-MINIMAL MULTIPLE TAN AREAS, UP TO 4 MM IN -PIGMENT, HEPATOCELLULAR, DIAMETER CENTRILOBULAR,-SLIGHT -MASS(ES); INVOLVING ENTIRE RIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL LATERAL LOBE: SINGLE MASS, 35 X 25 X / CENTRILOBULAR,-SLIGHT 15 MM, DARK BROWN AND SOFT; SAME ON -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / CUT SURFACE (CAP 22); REGIONAL LN EOSINOPHILIC CELL,-SLIGHT (HEPATIC) NORMAL (CAP 23) -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -NECROSIS, COAGULATIVE,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -VACUOLATION, HEPATOCELLULAR,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT 3690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1929 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93125 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:40 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; LEFT: 4 X 2 X 1 MM (RIGHT IS 5 -HYPERTROPHY, CORTICAL CELL,-MODERATE X 5 X 3 MM) -DEGENERATION, CYSTIC / HEMORRHAGIC,- -DIFFUSELY DARK; BILATERAL: DARK BROWN MODERATE -PIGMENT,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-HIBERNOMA,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 4 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM (CAP 24) -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1930 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93125 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:40 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE CERVIX: -M-SARCOMA, STROMAL,-NEOPLASM PRESENT SINGLE MASS, 23 X 17 X 17 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATELY SEVERE >NOTE:>HEPATIC LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; RIGHT: DARK RED (CAP -HEMORRHAGE,-PRESENT 25) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1931 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93125 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:40 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1932 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93126 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 406 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 221.6 GRAMS DATE AND TIME OF NECROPSY: 05/30/99 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >BEHAVIOR; HYPOACTIVE; MODERATE; CENTRILOBULAR POLYKARYOCYTOSIS VERIFIED AT NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-MODERATE >EYES; RED DISCHARGE; EYES; VERIFIED AT -GRANULAR CYTOPLASM, EOSINOPHILIC, NECROPSY: OBSERVED CENTRILOBULAR,-SLIGHT >DISCHARGE; NASAL; RED IN COLOR; -PIGMENT, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1933 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93126 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 406 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 221.6 GRAMS DATE AND TIME OF NECROPSY: 05/30/99 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 11 X 9 MM, RED, GREY, -B-ADENOMA,-NEOPLASM PRESENT TAN, LOBULATED AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DRY, RED MATERIAL -------------------------------------------- 3695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1934 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93126 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 406 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 221.6 GRAMS DATE AND TIME OF NECROPSY: 05/30/99 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1935 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93127 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 236.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:41 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0366 .0155 % .0181 WEIGHT TAKEN RT ADRENAL (AD1) .0326 .0138 % .0161 WEIGHT TAKEN BRAIN (BR) 2.0193 .8535 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2174 .5145 % .6029 WEIGHT TAKEN RT KIDNEY (KD1) 1.3144 .5555 % .6509 WEIGHT TAKEN LIVER (LI) 11.0447 4.6681 % 5.4696 WEIGHT TAKEN LUNG (LU) 1.3204 .5581 % .6539 WEIGHT TAKEN LF OVARY (OV0) .0707 .0299 % .0350 WEIGHT TAKEN RT OVARY (OV1) .0676 .0286 % .0335 WEIGHT TAKEN SPLEEN (SP) .4388 .1855 % .2173 WEIGHT TAKEN LF THYROID/PARA (TT0) .0078 .0033 % .0039 WEIGHT TAKEN RT THYROID/PARA (TT1) .0096 .0041 % .0048 WEIGHT TAKEN UTERUS (UT) 1.1344 .4795 % .5618 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID 3697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1936 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93127 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 236.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:41 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1937 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93128 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 7:53 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >RESPIRATION; LABORED; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -CYST,-PRESENT 3699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1938 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93128 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 7:53 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, BOTH ARE 1 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 3700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1939 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93128 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 7:53 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1940 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93129 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 296.0 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 8:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; PROTRUDING; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; DILATED PUPIL; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >EYES; DISCOLORED/LIGHT RED; EYES; -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -FIBROSIS,-MINIMAL 3702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1941 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93129 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 296.0 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 8:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 3703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1942 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93129 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 296.0 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 8:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1943 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93130 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 384.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:59 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0281 .0073 % .0123 WEIGHT TAKEN RT ADRENAL (AD1) .0275 .0072 % .0120 WEIGHT TAKEN BRAIN (BR) 2.2869 .5946 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.3284 .3454 % .5809 WEIGHT TAKEN RT KIDNEY (KD1) 1.2841 .3339 % .5615 WEIGHT TAKEN LIVER (LI) 13.9723 3.6329 % 6.1097 WEIGHT TAKEN LUNG (LU) 1.5891 .4132 % .6949 WEIGHT TAKEN LF OVARY (OV0) .0615 .0160 % .0269 WEIGHT TAKEN RT OVARY (OV1) .0384 .0100 % .0168 WEIGHT TAKEN SPLEEN (SP) .7788 .2025 % .3405 WEIGHT TAKEN LF THYROID/PARA (TT0) .0135 .0035 % .0059 WEIGHT TAKEN RT THYROID/PARA (TT1) .0129 .0034 % .0056 WEIGHT TAKEN UTERUS (UT) .5901 .1534 % .2580 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : UNVERIFIED AT NECROPSY -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1944 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93130 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 384.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:59 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1945 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93130 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 384.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:59 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1946 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 58; 50 X 50MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 68; 40 X 40MM; VERIFIED AT NECROPSY: -RAISED FOCUS(I)/AREA(S); ALONG MARGIN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED OF LEFT LATERAL LOBE: SINGLE DARK -HYPERTROPHY, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT BROWN, SLIGHTLY RAISED FOCUS, 4 MM IN CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: OBSERVED DIAMETER; COLLECTED ON ROUTINE SECTION OCCASIONALLY NOTED,-MODERATE >APPEARANCE; HUNCHED; VERIFIED AT -GRANULAR CYTOPLASM, EOSINOPHILIC, NECROPSY: NOT APPLICABLE CENTRILOBULAR,-SLIGHT >EYES; RED DISCHARGE; EYES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: OBSERVED PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -ANGIECTASIS,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1947 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 6 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, DARK BROWN, AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 50 X 45 X 30 MM, TAN, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT FIRM, AND VASCULAR; SOME AREAS EXUDE A WHITE CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) 3709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1948 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 70 X 55 X 35 MM, RED, TAN, VASCULAR, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; BILATERAL: DARK RED MATERIAL -------------------------------------------- 3710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1949 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1950 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- EYE (EY) : LEFT; VERIFIED AT NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; CONSISTS PRIMARILY OF FOCAL 30 X 40MM; VERIFIED AT NECROPSY: MINERALIZATION,-SLIGHT OBSERVED -INFLAMMATION, ACUTE,-MINIMAL >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE 3712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1951 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE SPLEEN (SP) : SPLEEN (SP) : -SMALL; 13 X 5 X 3 MM >UNREMARKABLE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,- MODERATELY SEVERE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, SEPTAL,-SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-SEVERE -MINERALIZATION,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, TAN, AND FIRM STOMACH, GL (ST) : -EROSION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 3713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1952 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: -HYPERPLASIA, EPITHELIAL, GLANDULAR,- MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MODERATE MM -MINERALIZATION, GLANDULAR MUCOSA,- MODERATE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); PERIANAL REGION: SINGLE -CYST / GALACTOCELE,-PRESENT MASS, 35 X 35 X 28 MM, RED, TAN AND -HYPERPLASIA, GLANDULAR,-MODERATELY FIRM; SAME ON CUT SURFACE; COLLECTED; SEVERE REGIONAL LN (INGUINAL) NORMAL (CAP -B-ADENOMA,-NEOPLASM PRESENT 21); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL) AS NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT CERVIX (CV) : CERVIX (CV) : -LARGE; 10 MM IN DIAMETER -HYPERPLASIA, STROMAL,-MODERATE VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: TAN -LUMEN, EXUDATE,-PRESENT MUCOID MATERIAL -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,- HINDFOOT: SINGLE DARK RED, MOIST MODERATELY SEVERE ABRADED AREA, 3 MM IN DIAMETER; -ACANTHOSIS / HYPERKERATOSIS,-MODERATE COLLECTED 3714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1953 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) ------------------------------------------------------------------------------------------------------------------------------------ 3715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1954 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93133 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 399.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT 3716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1955 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93133 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 399.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : -HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE),-MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,- NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1956 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93133 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 399.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1957 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93134 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 473 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/05/99 8:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); SHOULDER-LEFT; WEEK 48; 100 LIVER (LI) : X 100MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT 3719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1958 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93134 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 473 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/05/99 8:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 3 X 2 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL THORACIC -B-FIBROADENOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 118 X 100 X 70 MM (379.6 GRAMS),RED, DARK RED, GRAY, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -------------------------------------------- 3720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1959 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93134 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 473 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/05/99 8:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1960 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93135 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:43 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >APPEARANCE; MISSING; TEETH; VERIFIED CONSISTS PRIMARILY OF FOCAL AT NECROPSY: OBSERVED MINERALIZATION,-MINIMAL >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL 3722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1961 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93135 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:43 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 5 MM (RIGHT IS -ANGIECTASIS,-SEVERE 5 X 4 X 2 MM) -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL 3723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1962 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93135 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:43 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECT: SINGLE DARK RED AREA, 2 MM IN -HYPERPLASIA,-MINIMAL DIAMETER HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY FLUID FILLED -HYPERPLASIA, GLANDULAR,-MINIMAL CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: >UNREMARKABLE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 10 X 5 X 2 MM (CAP 23) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: -INFLAMMATION, CHRONIC-ACTIVE,- EACH HAS A SINGLE DARK RED, TAN, DRY MODERATELY SEVERE CRUSTED AREA, LEFT IS 7 X 5 MM, RIGHT -ACANTHOSIS / HYPERKERATOSIS,-MODERATE IS 4 X 2; BOTH COLLECTED -FIBROSIS,-MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1963 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93135 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:43 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; UPPER LEFT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER RIGHT INCISOR, BROKEN AT BASE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY -------------------------------------------- 3725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1964 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93135 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:43 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1965 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93136 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:50 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -LARGE; ALL LOBES THICKENED, MARGINS -FIBROSIS, PERIBILIARY,-SEVERE ROUNDED -DEGENERATION / NECROSIS, -DIFFUSELY DARK; ALL LOBES: DARK BROWN CENTRILOBULAR,-SLIGHT -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MULTIPLE, TAN, FIRM, SLIGHTLY -ECTASIA, BILE DUCT,-SEVERE INDISTINCT AREAS, UP TO 3 MM IN -STASIS, BILE,-PRESENT DIAMETER; COLLECTED ON ROUTINE SECTIONS -MASS(ES); MARGIN OF LEFT LATERAL LOBE: SINGLE MASS, 5 MM IN DIAMETER, RED, SOFT, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (SEE ANTERIOR MESENTERIC) -ROUGH SURFACE, DIFFUSE; ALL LOBES: ENTIRE LIVER HAS A LOBULATED APPEARANCE KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -PIGMENT, TUBULAR,-SLIGHT -ECTASIA, TUBULAR,-MODERATE -CAST, PROTEINACEOUS,-MODERATE SPLEEN (SP) : SPLEEN (SP) : -CYST(S); ALONG MARGIN: MULTIPLE RED -CYST, SEROSAL,-PRESENT FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER 3727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1966 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93136 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:50 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL OVARY (OV) : OVARY (OV) : -MASS(ES); LEFT, INVOLVING ENTIRE -HEMATOMA,-PRESENT OVARY: SINGLE MASS, 20 MM IN DIAMETER, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE RENAL) THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN FOCI, UP TO 1 MM IN MINIMAL DIAMETER -EDEMA, ALVEOLAR,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE >UNREMARKABLE TAN, ERODED AREA, 2 MM IN DIAMETER JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 3728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1967 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93136 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:50 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -HEPATIC ANOMALY / OBSTRUCTIVE HEPATOPATHY,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 20) -HEMORRHAGE,-PRESENT -DIFFUSELY RED; DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, RENAL (RL) : LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 8 X 5 -HYPERPLASIA, LYMPHOPLASMACYTIC,- X 3 MM (CAP 22) MODERATELY SEVERE -DIFFUSELY RED; BILATERAL: DARK RED LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -LARGE; ANTERIOR MESENTERIC: UP TO 10 -HEMORRHAGE,-PRESENT X 7 X 5 MM (CAP 21) -HYPERPLASIA, LYMPHOPLASMACYTIC,- -DIFFUSELY RED; ANTERIOR MESENTERIC: MODERATELY SEVERE DARK RED GENERAL COMMENT (GC) : -AUTOLYSIS-SEVERE -------------------------------------------- 3729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1968 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93136 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:50 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1969 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93137 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; MULTIPLE DARK RED AREAS, PINPOINT AND -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED UP TO 3 X 2 MM; COLLECTED ON ROUTINE CENTRILOBULAR POLYKARYOCYTOSIS >APPEARANCE; MISSING; TEETH; VERIFIED SECTIONS OCCASIONALLY NOTED,-SLIGHT AT NECROPSY: OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT 3731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1970 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93137 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >UNREMARKABLE DARK BROWN LINEAR AREA, 2 X 1 MM VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE HINDFEET: SINGLE RED ABRADED AREA ON EACH, LEFT IS 4 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING -------------------------------------------- 3732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1971 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93137 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1972 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93138 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0282 .0078 % .0132 WEIGHT TAKEN RT ADRENAL (AD1) .0301 .0083 % .0141 WEIGHT TAKEN BRAIN (BR) 2.1378 .5904 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.3720 .3789 % .6418 WEIGHT TAKEN RT KIDNEY (KD1) 1.3862 .3828 % .6484 WEIGHT TAKEN LIVER (LI) 13.3847 3.6964 % 6.2610 WEIGHT TAKEN LUNG (LU) 1.6428 .4537 % .7685 WEIGHT TAKEN LF OVARY (OV0) .0656 .0181 % .0307 WEIGHT TAKEN RT OVARY (OV1) .0778 .0215 % .0364 WEIGHT TAKEN SPLEEN (SP) .5241 .1447 % .2452 WEIGHT TAKEN LF THYROID/PARA (TT0) .0173 .0048 % .0081 WEIGHT TAKEN RT THYROID/PARA (TT1) .0157 .0043 % .0073 WEIGHT TAKEN UTERUS (UT) .6560 .1812 % .3069 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1973 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93138 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED SLIGHTLY RAISED AREA, -ANGIECTASIS,-PRESENT 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1974 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93138 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1975 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93139 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 300.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1976 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93139 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 300.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 6 X 5 MM (LEFT IS 5 -DEGENERATION, CYSTIC / HEMORRHAGIC,- X 5 X 4 MM) MODERATELY SEVERE -RED FOCUS(I)/AREAS(I); BILATERAL: -PIGMENT,-SLIGHT LEFT HAS A SINGLE DARK RED AREA, 3 MM IN DIAMETER, RIGHT HAS A MULTIPLE DARK RED AREAS, UP TO 7 X 4 MM HEART (HT) : -M-ENDOCARDIAL SCHWANNOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT MID >UNREMARKABLE ASPECT: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; -HYPERPLASIA, GLANDULAR,-MODERATE COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1977 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93139 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 300.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1978 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93140 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-RIGHT; EYE (EY) : VERIFIED AT NECROPSY: OBSERVED -DYSTROPHY, CORNEAL IN EARLY STAGES, >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; CONSISTS PRIMARILY OF FOCAL WEEK 93; 15 X 20MM; VERIFIED AT MINERALIZATION,-MINIMAL NECROPSY: OBSERVED BRAIN (BR) : BRAIN (BR) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT 102; 10 X 10MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); INGUINAL REGION-RIGHT; WEEK -NECROSIS, INDIVIDUAL HEPATOCYTE,- 104; 10 X 10MM; VERIFIED AT NECROPSY: SLIGHT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 3740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1979 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93140 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 2 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GREY, SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -INFLAMMATION, ACUTE,-MODERATE BROWN AREA, 3 X 2 MM -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -M-FIBROSARCOMA,- 4 NEOPLASMS PRESENT 3741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1980 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93140 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 15 X 15 X 10 MM, TAN, SOFT, AND LOBULATED; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 20 X 20 X 10 MM, TAN, SOFT, AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; RIGHT INGUINAL REGION, CAUDAL TO MASS #2: SINGLE MASS, 25 X 20 X 12 MM, TAN, GREY, FIRM; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT VENTRAL ABDOMINAL REGION: SINGLE MASS, 35 X 22 X 15 MM, TAN, GREY, LOBULATED, AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -DILATATION,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1981 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93140 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, AND DRY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1982 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93141 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 9:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; PERINEAL BRAIN (BR) : BRAIN (BR) : AREA; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; NO FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -HYPERTROPHY, HEPATOCELLULAR, >EYES; RED DISCHARGE; EYE-RIGHT; CENTRILOBULAR POLYKARYOCYTOSIS VERIFIED AT NECROPSY: OBSERVED OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL 3744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1983 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93141 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 9:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -ATROPHY,-MINIMAL STOMACH, GL (ST) : -EDEMA,-MINIMAL -INFLAMMATION, ACUTE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1984 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93141 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 9:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST -------------------------------------------- 3746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1985 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93141 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 9:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1986 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93142 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 210.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:31 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 8.2349 3.9170 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1987 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93142 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 210.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:31 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1988 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93143 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 264.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:18 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0368 .0139 % .0168 WEIGHT TAKEN RT ADRENAL (AD1) .0317 .0120 % .0145 WEIGHT TAKEN BRAIN (BR) 2.1882 .8289 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1879 .4500 % .5429 WEIGHT TAKEN RT KIDNEY (KD1) 1.2708 .4814 % .5808 WEIGHT TAKEN LIVER (LI) 11.0191 4.1739 % 5.0357 WEIGHT TAKEN LUNG (LU) 1.2593 .4770 % .5755 WEIGHT TAKEN LF OVARY (OV0) .1022 .0387 % .0467 WEIGHT TAKEN RT OVARY (OV1) .0792 .0300 % .0362 WEIGHT TAKEN SPLEEN (SP) .3885 .1472 % .1775 WEIGHT TAKEN LF THYROID/PARA (TT0) .0076 .0029 % .0035 WEIGHT TAKEN RT THYROID/PARA (TT1) .0087 .0033 % .0040 WEIGHT TAKEN UTERUS (UT) .4416 .1673 % .2018 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1989 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93143 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 264.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:18 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1990 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93144 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 175.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:10 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.9820 4.5472 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1991 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93144 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 175.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:10 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1992 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93145 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1993 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93145 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY RED; BILATERAL: DARK RED >UNREMARKABLE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -CONGESTION,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, TAN AND SOFT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERIANAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 30 X 20 X 15 MM, RED, -CYST / GALACTOCELE,-PRESENT DARK RED, GREEN, TAN, VASCULAR AND FIRM; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: RED; -HYPERPLASIA, LYMPHOPLASMACYTIC,- COLLECTED MODERATE 3755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1994 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93145 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1995 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93146 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:16 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 100; 20 X 20 MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); AXILLARY REGION-RIGHT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 102; 10 X 10MM; VERIFIED AT NECROPSY: -GRANULAR CYTOPLASM, EOSINOPHILIC, OBSERVED CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -DILATATION, PELVIC,-MODERATE -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE 3757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1996 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93146 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:16 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); RIGHT CORTICAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- SURFACE: SINGLE RED AREA, 4 MM IN MODERATE DIAMETER -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT CYST, 8 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 20 X 15 X 7 MM, TAN AND -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT FIRM; SAME ON CUT SURFACE; COLLECTED -HYPERPLASIA, GLANDULAR,-MODERATE INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 12 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN, CREAMY FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1997 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93146 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:16 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : >TISSUE MISSING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1998 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93146 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:16 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 1999 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93147 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 289.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0215 .0074 % .0091 WEIGHT TAKEN RT ADRENAL (AD1) .0259 .0089 % .0110 WEIGHT TAKEN BRAIN (BR) 2.3568 .8133 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2275 .4236 % .5208 WEIGHT TAKEN RT KIDNEY (KD1) 1.2747 .4399 % .5409 WEIGHT TAKEN LIVER (LI) 10.1724 3.5101 % 4.3162 WEIGHT TAKEN LUNG (LU) 1.6689 .5759 % .7081 WEIGHT TAKEN LF OVARY (OV0) .0595 .0205 % .0252 WEIGHT TAKEN RT OVARY (OV1) .0654 .0226 % .0277 WEIGHT TAKEN SPLEEN (SP) .6045 .2086 % .2565 WEIGHT TAKEN LF THYROID/PARA (TT0) .0150 .0052 % .0064 WEIGHT TAKEN RT THYROID/PARA (TT1) .0175 .0060 % .0074 WEIGHT TAKEN UTERUS (UT) .6163 .2127 % .2615 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL 3761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2000 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93147 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 289.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 20) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2001 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93147 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 289.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2002 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93148 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/15/00 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/15/00 8:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT >NOTE:>SEE SLIDE #21 ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-SLIGHT 3764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2003 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93148 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/15/00 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/15/00 8:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR -HYPERPLASIA,-SLIGHT ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : JEJUNUM (JE) : -MASS(ES); ATTACHED TO JEJUNUM, >AUTOLYTIC PROXIMAL TO DUODENUM: SINGLE MASS, 20 X 15 X 10 MM, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MESENTERIC) NORMAL ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 3765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2004 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93148 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/15/00 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/15/00 8:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -NEOPLASM, PANCREAS,-PRESENT -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2005 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 91; 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 100; 15 X 15MM; VERIFIED AT NECROPSY: -HYPERTROPHY, HEPATOCELLULAR, OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT 3767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2006 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 3 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT 3768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2007 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 28 X 20 X 15 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT DARK RED, TAN, BROWN AND FIRM; ON SECTION EXUDES A BROWN FLUID; (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 25 X 15 X 12 MM, DARK RED, BROWN, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (MANDIBULAR) NORMAL (CAP 25) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, -INFLAMMATION / NECROSIS, FAT,-MODERATE NEAR RIGHT UTERINE HORN: SINGLE MASS, 20 X 10 X 8 MM, BROWN, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 26); REGIONAL LN (LUMBAR) NORMAL (CAP 27) 3769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2008 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, MANDIBULAR (MN) ------------------------------------------------------------------------------------------------------------------------------------ 3770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2009 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93150 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 290.3 GRAMS DATE AND TIME OF NECROPSY: 01/19/00 14:21 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED -HYPERTROPHY, HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >DISCHARGE; NASAL; RED IN COLOR; OCCASIONALLY NOTED,-MODERATE VERIFIED AT NECROPSY: OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 3771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2010 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93150 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 290.3 GRAMS DATE AND TIME OF NECROPSY: 01/19/00 14:21 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -------------------------------------------- 3772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2011 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93150 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 290.3 GRAMS DATE AND TIME OF NECROPSY: 01/19/00 14:21 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2012 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93151 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:31 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 79; 70 X 100MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >MASS(ES); AXILLARY REGION-LEFT; WEEK CENTRILOBULAR POLYKARYOCYTOSIS 89; 30 X 20MM; VERIFIED AT NECROPSY: OCCASIONALLY NOTED,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT IS 4 MM IN DIAMETER) -DEGENERATION, CYSTIC / HEMORRHAGIC,- -DIFFUSELY DARK; BILATERAL: DARK BROWN MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT -HYPERPLASIA, GRANULOSA CELL,-MODERATE 3774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2013 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93151 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:31 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT -DARK FOCUS(I)/AREA(S); ALONG ENTIRE -HYPERPLASIA,-SLIGHT LEFT ASPECT: MULTIPLE BLACK FOCI, UP TO 2 MM IN DIAMETER STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO -EROSION,-MINIMAL BLACK AREAS, ONE IS 3 X 1 MM, OTHER IS 2 X 1 MM RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 3775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2014 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93151 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:31 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); INVOLVING ENTIRE INGUINAL REGION: SINGLE MASS, 110 X 83 X 50 MM (243.6 GRAMS), RED, DARK RED, GRAY, TAN, LOBULATED, VASCULAR, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 21 X 20 X 13 MM, RED, GRAY, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE AXILLARY) UTERUS (UT) : UTERUS (UT) : -MASS(ES); LEFT WITHIN LUMEN, -B-POLYP, ENDOMETRIAL, STROMAL,- EXTENDING INTO BIFURCATION: SINGLE NEOPLASM PRESENT DARK RED AND GELATINOUS MASS, 11 X 10 -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- X 9 MM; ON SECTION EXUDES A DARK RED SLIGHT GRANULAR MATERIAL; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 25) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 6 X 4 X 3 MM -HEMORRHAGE,-PRESENT (RIGHT IS UP TO 3 X 3 X 2 MM) (CAP 24) -HYPERPLASIA, LYMPHOPLASMACYTIC,- -DIFFUSELY RED; LEFT: DARK RED (CAP MODERATE 24) LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2015 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93151 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:31 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN -------------------------------------------- 3777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2016 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93151 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:31 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2017 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93152 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 53; 25 X 25MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; HUNCHED; VERIFIED AT LIVER (LI) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >DISCHARGE; NASAL; RED IN COLOR; OCCASIONALLY NOTED,-MODERATE VERIFIED AT NECROPSY: OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, >EYES; RED DISCHARGE; EYES; VERIFIED AT CENTRILOBULAR,-MINIMAL NECROPSY: OBSERVED -PIGMENT, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT CENTRILOBULAR,-MINIMAL NECROPSY: OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2018 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93152 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 8 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT GRAY, AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 30 X 21 X 14 MM, DARK -CYST / GALACTOCELE,-PRESENT RED, TAN, LOBULATED, AND FIRM; SAME ON -INFLAMMATION, CHRONIC,-MODERATE CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 9 X 5 X 3 MM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2019 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93152 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DARK RED, DRY CRUSTED MATERIAL -------------------------------------------- 3781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2020 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93152 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2021 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93153 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:44 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); DORSAL; WEEK 91; 40 X 40MM; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: OBSERVED CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2022 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93153 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:44 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MODERATE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM -B-ADENOMA,-NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); DIFFUSE: -HYPERPLASIA,-MODERATE MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); MID DORSAL THORACIC REGION: -HYPERPLASIA, GLANDULAR,-MODERATE SINGLE MASS, 45 X 36 X 27 MM, TAN, >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT 3784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2023 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93153 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:44 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE HINDFEET: SINGLE RED ABRADED AREA, -ULCERATION,-MODERATE LEFT IS 10 X 7 MM, RIGHT IS 3 MM IN -ACANTHOSIS / HYPERKERATOSIS,-MODERATE DIAMETER; COLLECTED -HYPERPLASIA, BONE,-MODERATE JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: UP TO 10 MM -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2024 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93153 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:44 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2025 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93154 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0398 .0127 % .0168 WEIGHT TAKEN RT ADRENAL (AD1) .0328 .0104 % .0138 WEIGHT TAKEN BRAIN (BR) 2.3753 .7553 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.2854 .4087 % .5412 WEIGHT TAKEN RT KIDNEY (KD1) 1.4335 .4558 % .6035 WEIGHT TAKEN LIVER (LI) 13.5315 4.3025 % 5.6968 WEIGHT TAKEN LUNG (LU) 1.5496 .4927 % .6524 WEIGHT TAKEN LF OVARY (OV0) .0476 .0151 % .0200 WEIGHT TAKEN RT OVARY (OV1) .0551 .0175 % .0232 WEIGHT TAKEN SPLEEN (SP) .5400 .1717 % .2273 WEIGHT TAKEN LF THYROID/PARA (TT0) .0062 .0020 % .0026 WEIGHT TAKEN RT THYROID/PARA (TT1) .0103 .0033 % .0043 WEIGHT TAKEN UTERUS (UT) 1.0091 .3209 % .4248 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL 3787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2026 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93154 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -HEMORRHAGE,-PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM; APPEARS SWOLLEN >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL -SECRETORY MATERIAL,-PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2027 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93154 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2028 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93155 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 224.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:57 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0387 .0172 % .0226 WEIGHT TAKEN RT ADRENAL (AD1) .0397 .0177 % .0232 WEIGHT TAKEN BRAIN (BR) 1.7141 .7639 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.0546 .4700 % .6152 WEIGHT TAKEN RT KIDNEY (KD1) 1.0768 .4799 % .6282 WEIGHT TAKEN LIVER (LI) 9.4384 4.2061 % 5.5063 WEIGHT TAKEN LUNG (LU) 1.3608 .6064 % .7939 WEIGHT TAKEN LF OVARY (OV0) .0660 .0294 % .0385 WEIGHT TAKEN RT OVARY (OV1) .0609 .0271 % .0355 WEIGHT TAKEN SPLEEN (SP) .4271 .1903 % .2492 WEIGHT TAKEN LF THYROID/PARA (TT0) .0073 .0033 % .0043 WEIGHT TAKEN RT THYROID/PARA (TT1) .0086 .0038 % .0050 WEIGHT TAKEN UTERUS (UT) .5279 .2352 % .3080 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN -------------------------------------------- 3790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2029 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93155 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 224.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:57 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2030 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93156 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:21 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -PROTRUDING; RIGHT: 4 MM FROM ORBIT; -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE >SKIN & PELAGE; SORE/SCAB; VENTRAL- TISSUE SURROUNDING EYE IS DRY AND TAN; CERVICAL; VERIFIED AT NECROPSY: POSSIBLY CONSISTENT WITH SCAB (RIGHT OBSERVED EYE) AS NOTED IN CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 53; LIVER (LI) : LIVER (LI) : 40 X 50MM; VERIFIED AT NECROPSY: -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED MULTIPLE TAN AREAS, UP TO 8 X 3 MM; -HYPERTROPHY, HEPATOCELLULAR, >MASS(ES); VENTRAL-ABDOMINAL; WEEK 84; COLLECTED ON ROUTINE SECTIONS CENTRILOBULAR POLYKARYOCYTOSIS 10 X 10MM; VERIFIED AT NECROPSY: OCCASIONALLY NOTED,-SLIGHT OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, >EYES; PROTRUDING; EYE-RIGHT; VERIFIED CENTRILOBULAR,-MINIMAL AT NECROPSY: OBSERVED -PIGMENT, HEPATOCELLULAR, >EYES; SCAB; EYE-RIGHT; VERIFIED AT CENTRILOBULAR,-MINIMAL NECROPSY: OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- >EYES; PALE; EYE-RIGHT; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT 3792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2031 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93156 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:21 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 5 MM IN DIAMETER -HEMORRHAGE,-PRESENT -DIFFUSELY RED; DARK RED -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE BONE, FEMUR (FE) : -HYPERPLASIA, CARTILAGE,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -N-CARCINOMA,-FROM MAMMARY, FEMALE MULTIPLE TAN FOCI, UP TO 3 MM IN -INFILTRATE, MACROPHAGE, ALVEOLAR,- DIAMETER SLIGHT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK RED FOCUS, 4 MM IN DIAMETER HARDERIAN GLAND (HG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2032 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93156 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:21 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ HARDERIAN GLAND (HG) : -MASS(ES); INVOLVING ENTIRE RIGHT: SINGLE MASS, 15 X 10 X 6 MM, RED, TAN, GREY, GELATINOUS AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) SALIV GL, MANDIB (SG) : SALIV GL, MANDIB (SG) : -SMALL; LEFT: 11 X 8 X 3 MM (RIGHT IS -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO 20 X 9 X 4 MM) NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); PERIANAL REGION: SINGLE TAN -M-CARCINOMA,- 2 NEOPLASMS PRESENT AND FIRM MASS, 22 X 16 X 11 MM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL- ABDOMINAL REGION) AS NOTED IN CLINICAL OBSERVATIONS -MASS #2; VENTRAL CERVICAL REGION, EXTENDING TO LEFT PECTORAL REGION: SINGLE MASS, 77 X 38 X 30 MM, RED, DARK RED, GREY, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR); OVERLYING SKIN HAS A SINGLE MOIST, RED AND TAN ERODED AREA, 21 X 20 MM; COLLECTED WITH MASS UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE >UNREMARKABLE 3794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2033 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93156 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:21 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 11 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 7 X 6 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2034 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93156 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:21 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2035 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93157 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 537.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-FIBROMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2036 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93157 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 537.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN, >UNREMARKABLE 17 MM FROM BIFURCATION: SINGLE RED FLUID FILLED CYST, 2 MM IN DIAMETER VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); WITHIN ADIPOSE TISSUE NEAR -INFLAMMATION / NECROSIS, FAT,-SEVERE BIFURCATION OF UTERUS: SINGLE MASS, 25 X 20 X 14 MM, GREEN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) 3798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2037 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93157 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 537.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2038 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93158 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:13 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; BRAIN (BR) : BRAIN (BR) : WEEK 71; 70 X 60MM; VERIFIED AT -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE 3800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2039 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93158 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:13 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT IS 5 -ANGIECTASIS,-SLIGHT X 4 X 3 MM) -HYPERTROPHY, CORTICAL CELL,-SLIGHT -MOTTLED; BILATERAL: DARK BROWN AND -PIGMENT,-MINIMAL TAN -B-ADENOMA,-NEOPLASM PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT -CYST,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 90 X 60 X 25 MM (95.3 -B-FIBROADENOMA,-NEOPLASM PRESENT GRAMS), RED, TAN, SOFT, LOBULATED, AND -HYPERPLASIA, GLANDULAR,-SLIGHT VASCULAR; SAME ON CUT SURFACE; >NOTE:>MASS #1 IS THE CARCINOMA COLLECTED; REGIONAL LN (SEE INGUINAL) -MASS #2; RIGHT PERINEAL REGION: SINGLE MASS, 30 X 25 X 25 MM, TAN, DARK BROWN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2040 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93158 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:13 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, INGUINAL (IN) : LN, INGUINAL (IN) : -DIFFUSELY RED; RIGHT: DARK RED (CAP -INFILTRATE, MACROPHAGE, PIGMENTED,- 22); LEFT (NOT IDENTIFIED) MODERATE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2041 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93158 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:13 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2042 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93159 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 173.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:00 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) -------- -------- -------- NOT TAKEN RT ADRENAL (AD1) -------- -------- -------- NOT TAKEN BRAIN (BR) -------- -------- -------- NOT TAKEN LF KIDNEY (KD0) -------- -------- -------- NOT TAKEN RT KIDNEY (KD1) -------- -------- -------- NOT TAKEN LIVER (LI) 7.2825 4.2009 % -------- WEIGHT TAKEN LUNG (LU) -------- -------- -------- NOT TAKEN LF OVARY (OV0) -------- -------- -------- NOT TAKEN RT OVARY (OV1) -------- -------- -------- NOT TAKEN SPLEEN (SP) -------- -------- -------- NOT TAKEN LF THYROID/PARA (TT0) -------- -------- -------- NOT TAKEN RT THYROID/PARA (TT1) -------- -------- -------- NOT TAKEN UTERUS (UT) -------- -------- -------- NOT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -------------------------------------------- 3804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2043 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93159 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 173.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:00 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) ------------------------------------------------------------------------------------------------------------------------------------ 3805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2044 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93160 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:57 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); NOSE; WEEK 72; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, >SKIN & PELAGE; YELLOW HAIR COAT; PERIPORTAL,-SLIGHT VERIFIED AT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; -FIBROSIS, PERIBILIARY,-SLIGHT VERIFIED AT NECROPSY: NOT OBSERVED -INFILTRATE, MACROPHAGE, PIGMENTED,- >APPEARANCE; THIN; VERIFIED AT MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : >APPEARANCE; SWOLLEN; VENTRAL- -PIGMENT, TUBULAR,-MODERATE ABDOMINAL; VERIFIED AT NECROPSY: SPLEEN (SP) : OBSERVED -PIGMENT, INCREASED,-MODERATE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; PANCREAS (PA) : VERIFIED AT NECROPSY: NOT APPLICABLE -ZYMOGEN GRANULES, DECREASED,-MODERATE >EXCRETION; FEW FECES; VERIFIED AT ADRENAL, CORTEX (AC) : NECROPSY: NOT APPLICABLE -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE 3806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2045 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93160 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:57 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL RECTUM (RE) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-MODERATE SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); ATTACHED TO RIGHT -ACANTHOSIS/HYPERKERATOSIS,-MODERATE NOSTRIL: SINGLE BROWN, DRY, CRUSTED, -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE WART-LIKE AREA, 5 X 1 X 1 MM (CAP 21) -SUPERFICIAL CRUSTING,-MINIMAL CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT >NOTE:>PERITONITIS CAVITY, ABDOM (PC) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL 3807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2046 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93160 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:57 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; INVOLVING CECUM, COLON, AND RECTUM: DISTENDED UP TO 27 MM IN DIAMETER AND FILLED WITH A HARD FECAL LIKE MATERIAL; POSSIBLY CONSISTENT WITH SWOLLEN ABDOMEN NOTED IN CLINICAL OBSERVATIONS -------------------------------------------- 3808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2047 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93160 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:57 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2048 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93161 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- LIVER (LI) : LIVER (LI) : LEFT; VERIFIED AT NECROPSY: NOT -CYST(S); ALL LOBES: MULTIPLE CLEAR -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED FLUID FILLED CYSTS, UP TO 1 MM IN -HYPERTROPHY, HEPATOCELLULAR, DIAMETER; COLLECTED ON ROUTINE CENTRILOBULAR POLYKARYOCYTOSIS SECTIONS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : SPLEEN (SP) : -LARGE; 55 X 11 X 7 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : PANCREAS (PA) : -GELATINOUS; DIFFUSE: CLEAR AND TAN -FIBROSIS,-SEVERE -LIGHT FOCUS(I)/AREA(S); DIFFUSE: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE MULTIPLE TAN AND FIRM AREAS, UP TO 4 MM IN DIAMETER ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL 3810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2049 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93161 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -LARGE; UP TO 20 X 13 X 11 MM; ALL -LYMPHANGIECTASIS,-MODERATE APPEAR CYSTIC MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -ADHESION(S); RIGHT MIDDLE LOBE -INFILTRATE, MACROPHAGE, ALVEOLAR,- ADHERED TO RIGHT CAUDAL LOBE: MULTIPLE SLIGHT GREY FIBROUS ADHESIONS -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT ILEUM (IL) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CECUM (CE) : CECUM (CE) : -LARGE; 60 X 30 X 7 MM; FILLED WITH A -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE DARK GREEN, SOLID, FECAL-LIKE MATERIAL COLON (CO) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE RECTUM (RE) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-MODERATE AND FIRM; COLLECTED ON ROUTINE SECTION 3811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2050 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93161 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -LARGE; UP TO 11 X 10 X 5 MM; ALL -HEMORRHAGE,-PRESENT APPEAR CYSTIC (CAP 23) -LYMPHANGIECTASIS,-MODERATELY SEVERE FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE DARK RED, DRY CRUSTED AREA, 3 X MODERATELY SEVERE 2 MM; COLLECTED -ULCERATION,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -BACTERIA,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2051 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93161 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2052 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93162 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MISSING; TEETH; VERIFIED BRAIN (BR) : AT NECROPSY: OBSERVED -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); INGUINAL REGION-LEFT; WEEK -HYPERTROPHY, HEPATOCELLULAR, 102; 10 X 10MM; VERIFIED AT NECROPSY: CENTRILOBULAR POLYKARYOCYTOSIS OBSERVED OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 3814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2053 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93162 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 MM IN DIAMETER (RIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE IS 3 MM IN DIAMETER) -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 3 -UNILATERALLY EXAMINED,-PRESENT MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 6 X 4 MM MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -RED FOCUS(I)/AREA(S); POSTERIOR -ANGIECTASIS,-PRESENT ASPECT: SINGLE DARK RED AREA, 3 X 2 MM MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL 3815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2054 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93162 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 32 X 23 X 15 MM, TAN, RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING LN, ANT MES/PANC (AP) : LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 24) >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PANCREAS FOR DIAGNOSIS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: MISSING -------------------------------------------- 3816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2055 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93162 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2056 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93163 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 310.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:57 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0311 .0100 % .0147 WEIGHT TAKEN RT ADRENAL (AD1) .0283 .0091 % .0133 WEIGHT TAKEN BRAIN (BR) 2.1210 .6822 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.4342 .4613 % .6762 WEIGHT TAKEN RT KIDNEY (KD1) 1.4052 .4520 % .6625 WEIGHT TAKEN LIVER (LI) 14.7661 4.7495 % 6.9619 WEIGHT TAKEN LUNG (LU) 1.4413 .4636 % .6795 WEIGHT TAKEN LF OVARY (OV0) .0617 .0198 % .0291 WEIGHT TAKEN RT OVARY (OV1) .0585 .0188 % .0276 WEIGHT TAKEN SPLEEN (SP) .4675 .1504 % .2204 WEIGHT TAKEN LF THYROID/PARA (TT0) .0095 .0031 % .0045 WEIGHT TAKEN RT THYROID/PARA (TT1) .0100 .0032 % .0047 WEIGHT TAKEN UTERUS (UT) 1.4084 .4530 % .6640 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DILATATION, PELVIC,-MINIMAL 3818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2057 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93163 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 310.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:57 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -CYST(S); ALONG MARGIN OF CAUDAL END: -PIGMENT, INCREASED,-MODERATE SINGLE CLEAR FLUID FILLED CYST, 2 MM -FIBROSIS, CAPSULAR,-PRESENT IN DIAMETER >NOTE:>FIBROUS TAGS POSSIBLY REMAINS OF CYST WALLS ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL -SECRETORY MATERIAL,-PRESENT UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2058 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93163 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 310.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:57 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2059 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93164 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:08 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 77; 65 X 40MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; BLUE SKIN; VENTRAL- -RAISED FOCUS(I)/AREA(S); -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN -HYPERTROPHY, HEPATOCELLULAR, NOT APPLICABLE LOBE: SINGLE DARK RED AREA, 2 MM IN CENTRILOBULAR POLYKARYOCYTOSIS DIAMETER; COLLECTED ON ROUTINE SECTION OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -CYST, BILIARY,-PRESENT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SEVERE 3821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2060 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93164 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:08 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ KIDNEY (KD) : -HYPERPLASIA, TRANSITIONAL CELL,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, -M-CARCINOMA,-NEOPLASM PRESENT EXTENDING INTO LEFT PERIANAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 65 X 45 X 30 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, TAN, FIRM AND LOBULATED; -HYPERPLASIA, GLANDULAR,-MINIMAL SOME AREAS EXUDE A DARK BROWN >NOTE:>FIBROADENOMA IS ON SLIDE 22; NOT SEMIFLUID; PORTION COLLECTED; REGIONAL NOTED MACROSCOPICALLY MASS 1 IS LN (INGUINAL) NOT IDENTIFIED THE CARCINOMA 3822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2061 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93164 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:08 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -CYST(S); RIGHT INGUINAL REGION: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 20 MM IN DIAMETER (CAP 22) >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF MID LEFT -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- HORN: SINGLE MASS, 3 MM IN DIAMETER, SLIGHT TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2062 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93164 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:08 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2063 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93165 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:53 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC; WEEK 93; BRAIN (BR) : BRAIN (BR) : 30 X 30MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED -MINERALIZATION,-MINIMAL >SKIN & PELAGE; SORE/SCAB; DORSAL- LIVER (LI) : LIVER (LI) : CERVICAL; VERIFIED AT NECROPSY: -MOTTLED; ALL LOBES: RED AND TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >SKIN & PELAGE; SORE/SCAB; DORSAL- CENTRILOBULAR POLYKARYOCYTOSIS CERVICAL-LEFT; VERIFIED AT NECROPSY: OCCASIONALLY NOTED,-SLIGHT OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, >SKIN & PELAGE; SORE/SCAB; DORSAL- CENTRILOBULAR,-SLIGHT CERVICAL-RIGHT; VERIFIED AT NECROPSY: -PIGMENT, HEPATOCELLULAR, OBSERVED CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL 3825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2064 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93165 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:53 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -N-CARCINOMA,-FROM MAMMARY, FEMALE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK -ANGIECTASIS,-PRESENT RED, GRAY, AND SOFT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); BILATERAL SHOULDER -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT REGIONS, DIFFUSE: MULTIPLE BROWN DRY -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CRUSTED AREAS, UP TO 5 X 2 MM; -ULCERATION,-MINIMAL CONSISTENT WITH ALL SCABS NOTED IN CLINICAL OBSERVATIONS (CAP 23) 3826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2065 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93165 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:53 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 40 X 30 X 24 MM, DARK -CYST / GALACTOCELE,-PRESENT RED, TAN, GRAY, BROWN, AND LOBULATED; -HYPERPLASIA, GLANDULAR,-MINIMAL ON SECTION EXUDES A BROWN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, DIFFUSE: MULTIPLE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- CLEAR FLUID FILLED CYSTS, UP TO 4 MM MODERATELY SEVERE IN DIAMETER CERVIX (CV) : CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE ORGAN: -M-SARCOMA, STROMAL,-NEOPLASM PRESENT SINGLE MASS, 30 X 23 X 20 MM, RED, -HEMORRHAGE,-PRESENT DARK RED, TAN, GRAY, LOBULATED, SOFT, AND GELATINOUS; ON SECTION EXUDES A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 22) >UNREMARKABLE LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2066 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93165 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:53 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2067 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93166 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:11 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT MULTIPLE DARK RED AREAS, PINPOINT AND -VACUOLATION, HEPATOCELLULAR, MIDZONAL UP TO 3 X 2 MM; COLLECTED ON ROUTINE / CENTRILOBULAR,-SLIGHT SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / -RAISED FOCUS(I)/AREA(S); ONE EOSINOPHILIC CELL,-SLIGHT PAPILLARY PROCESS: SINGLE BROWN AREA, -FOCUS, ALTERED HEPATOCELLULAR, 6 X 6 X 1 MM (CAP 22) BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); RIGHT: SINGLE -HYPERTROPHY, CORTICAL CELL,-SLIGHT DARK RED AREA, 3 MM IN DIAMETER -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT -PIGMENT,-MINIMAL 3829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2068 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93166 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:11 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, RIGHT IS 3 MM IN DIAMETER, LEFT IS 9 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE WHITE AREAS, UP TO 12 X 10 MM SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -MASS(ES); RIGHT INGUINAL REGION: -CYST, EPIDERMAL INCLUSION,-PRESENT SINGLE MASS, 12 X 12 X 8 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2069 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93166 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:11 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN WALLS, -B-POLYP, ENDOMETRIAL, STROMAL,- NEAR BIFURCATION: SINGLE CLEAR FLUID NEOPLASM PRESENT FILLED CYST, 13 X 5 X 5 MM VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2070 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93166 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:11 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2071 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93167 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 225.0 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 14:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT OBSERVED -MOTTLED; ALL LOBES: TAN AND BROWN -VACUOLATION, HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT PERIPORTAL,-SEVERE NECROPSY: NOT APPLICABLE KIDNEY (KD) : KIDNEY (KD) : >SKIN & PELAGE; ROUGH HAIR COAT; -ROUGH SURFACE, DIFFUSE; BILATERAL -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE MINIMAL >SKIN & PELAGE; YELLOW HAIR COAT; -MINERALIZATION, PELVIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED -VACUOLATION, TUBULAR EPITHELIUM,- >SKIN & PELAGE; PALE; ENTIRE BODY; SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE SPLEEN (SP) : SPLEEN (SP) : >APPEARANCE; THIN; VERIFIED AT -SMALL; 25 X 8 X 6 MM -PIGMENT, INCREASED,-MINIMAL NECROPSY: OBSERVED PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,- MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2072 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93167 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 225.0 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 14:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ESOPHAGUS (ES) : -HYPERKERATOSIS,-SLIGHT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT TAN ERODED AREAS, UP TO 3 X 2 MM -INFLAMMATION, SUBACUTE,-SLIGHT -ULCERATION,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -------------------------------------------- 3834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2073 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93167 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 225.0 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 14:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2074 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93168 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL 3836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2075 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93168 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER -UNILATERALLY EXAMINED,-PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -MINERALIZATION, VASCULAR,-MINIMAL MULTIPLE TAN AREAS, UP TO 6 X 4 MM -INFILTRATE, MACROPHAGE, ALVEOLAR,- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE -INFLAMMATION / NECROSIS, FAT,-MODERATE OF RIGHT OVARY: SINGLE MASS, 18 X 8 X 8 MM, TAN, GREEN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (LUMBAR) NORMAL (CAP 23) 3837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2076 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93168 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2077 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93169 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 232.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:51 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0281 .0121 % .0141 WEIGHT TAKEN RT ADRENAL (AD1) .0250 .0107 % .0125 WEIGHT TAKEN BRAIN (BR) 1.9996 .8589 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1705 .5028 % .5854 WEIGHT TAKEN RT KIDNEY (KD1) 1.1404 .4899 % .5703 WEIGHT TAKEN LIVER (LI) 10.6066 4.5561 % 5.3044 WEIGHT TAKEN LUNG (LU) 1.0841 .4657 % .5422 WEIGHT TAKEN LF OVARY (OV0) .0604 .0259 % .0302 WEIGHT TAKEN RT OVARY (OV1) .0631 .0271 % .0316 WEIGHT TAKEN SPLEEN (SP) .4329 .1860 % .2165 WEIGHT TAKEN LF THYROID/PARA (TT0) .0062 .0027 % .0031 WEIGHT TAKEN RT THYROID/PARA (TT1) .0092 .0040 % .0046 WEIGHT TAKEN UTERUS (UT) 1.3993 .6011 % .6998 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DARK BROWN FOCUS, 2 MM IN DIAMETER 3839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2078 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93169 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 232.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:51 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) ------------------------------------------------------------------------------------------------------------------------------------ 3840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2079 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93170 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 340.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; HEAD TILT; RIGHT; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EYES; RED DISCHARGE; EYE-LEFT; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT -MASS(ES); ONE PAPILLARY PROCESS, -HYPERTROPHY, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE INVOLVING ENTIRE LOBE: SINGLE MASS, 20 CENTRILOBULAR POLYKARYOCYTOSIS X 15 X 10 MM, RED, DARK RED, BROWN, OCCASIONALLY NOTED,-SLIGHT VASCULAR AND FIRM; SAME ON CUT SURFACE -NECROSIS, INDIVIDUAL HEPATOCYTE,- (CAP 21); REGIONAL LN (HEPATIC) NORMAL MINIMAL (CAP 22) -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -M-CARCINOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 10 MM IN DIAMETER 3841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2080 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93170 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 340.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); RIGHT CAUDAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- LOBE: MULTIPLE TAN AREAS, UP TO 10 X 5 SLIGHT MM HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 13 X 7 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GRAY AND SOFT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>SEE SLIDE #19 VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MODERATE 3842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2081 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93170 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 340.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2082 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93171 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 350.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:35 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN ORGAN NAME (GRAMS) TO BODY WEIGHT (%) WEIGHT RATIO STATUS ---------------------- -------- -------- -------- ----------- LF ADRENAL (AD0) .0262 .0075 % .0122 WEIGHT TAKEN RT ADRENAL (AD1) .0341 .0097 % .0159 WEIGHT TAKEN BRAIN (BR) 2.1395 .6102 % 1.0000 WEIGHT TAKEN LF KIDNEY (KD0) 1.1620 .3314 % .5431 WEIGHT TAKEN RT KIDNEY (KD1) 1.1783 .3361 % .5507 WEIGHT TAKEN LIVER (LI) 13.5288 3.8588 % 6.3233 WEIGHT TAKEN LUNG (LU) 1.4643 .4177 % .6844 WEIGHT TAKEN LF OVARY (OV0) .0765 .0218 % .0358 WEIGHT TAKEN RT OVARY (OV1) .0678 .0193 % .0317 WEIGHT TAKEN SPLEEN (SP) .4089 .1166 % .1911 WEIGHT TAKEN LF THYROID/PARA (TT0) .0117 .0033 % .0055 WEIGHT TAKEN RT THYROID/PARA (TT1) .0214 .0061 % .0100 WEIGHT TAKEN UTERUS (UT) 1.2207 .3482 % .5706 WEIGHT TAKEN ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL 3844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2083 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93171 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 350.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:35 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); RIGHT CAUDAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- LOBE: TWO TAN AREAS, ONE IS 3 X 2 MM, SLIGHT THE OTHER IS 2 X 1 MM PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 4 X 4 MM; APPEARS SWOLLEN >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-MINIMAL CLEAR FLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -------------------------------------------- 3845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2084 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93171 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 350.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:35 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2085 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93172 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 212.6 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 14:31 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; SQUINTED; PERI-ORBITAL-RIGHT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HEAD TILT; LEFT; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >EXCRETION; FEW FECES; VERIFIED AT -LIGHT FOCUS(I)/AREA(S); VISCERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE SURFACE OF LEFT LATERAL LOBE: SINGLE -HYPERTROPHY, HEPATOCELLULAR, >APPEARANCE; THIN; VERIFIED AT TAN FOCUS, 1 MM IN DIAMETER; COLLECTED CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: OBSERVED ON ROUTINE SECTION OCCASIONALLY NOTED,-MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT -LIPIDOSIS, SUBCAPSULAR (TENSION NECROPSY: NOT APPLICABLE LIPIDOSIS),-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >EYES; RED DISCHARGE; EYE-RIGHT; -MINERALIZATION, PELVIC,-MINIMAL VERIFIED AT NECROPSY: OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT >SECTION EXAMINED; TISSUE NOT PRESENT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED 3847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2086 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93172 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 212.6 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 14:31 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL ESOPHAGUS (ES) : ESOPHAGUS (ES) : -MASS(ES); 11 MM FROM EPIGLOTTIS, >UNREMARKABLE ATTACHED TO WALL WITHIN LUMEN: SINGLE TAN AND FIRM MASS, 2 X 2 X 1 MM; CUT SURFACE HAS A FIRM PLASTIC-LIKE AREA, 1 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 21) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 14 X 13 X 10 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GREY, LOBULATED AND SOFT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : >TISSUE MISSING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY, RED MATERIAL -ANIMAL THIN 3848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2087 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93172 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 212.6 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 14:31 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2088 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93173 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:17 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); SHOULDER-RIGHT; WEEK 88; 20 BRAIN (BR) : BRAIN (BR) : X 30MM; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >DISCHARGE; NASAL; RED IN COLOR; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >EXCRETION; FEW FECES; VERIFIED AT CENTRILOBULAR POLYKARYOCYTOSIS NECROPSY: NOT APPLICABLE OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE 3850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2089 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93173 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:17 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DIFFUSELY RED; LEFT: DARK RED -HYPERTROPHY, CORTICAL CELL,-MODERATE -SMALL; RIGHT: 3 X 3 X 2 MM (LEFT IS 5 -DEGENERATION, CYSTIC / HEMORRHAGIC,- X 4 X 3 MM) MODERATE -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 6 MM IN SLIGHT DIAMETER HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; RIGHT: 4 X 3 X 3 MM (LEFT IS 3 -B-ADENOMA, C-CELL,-NEOPLASM PRESENT X 2 X 1 MM) PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, BROWN, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, VASCULAR AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE DARK RED AREAS, UP TO 3 X 1 MM 3851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2090 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93173 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:17 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT LATERAL CERVICAL -M-CARCINOMA,-NEOPLASM PRESENT REGION: SINGLE MASS, 30 X 20 X 20 MM, -CYST / GALACTOCELE,-PRESENT DARK RED, RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT CONTAINS A LIGHT GREEN SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -DISCHARGE-NASAL; DARK RED, DRY MATERIAL 3852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2091 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93173 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/28/00 STUDY DAY OF DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 03/28/00 11:17 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2092 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93174 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >MASS(ES); VENTRAL-THORACIC; WEEK 100; -COMPRESSION, VENTRAL,-PRESENT 30 X 20 MM; VERIFIED AT NECROPSY: LIVER (LI) : OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 3854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2093 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93174 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: -HYPERPLASIA, GLANDULAR,-MODERATELY SINGLE MASS, 45 X 30 X 15 MM, TAN AND SEVERE SOFT; SAME ON CUT SURFACE; (CAP 22); >NOTE:>SEE ALSO SKIN REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CERVIX (CV) : CERVIX (CV) : -LARGE; 8 MM IN DIAMETER -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2094 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93174 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2095 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93175 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS DATE AND TIME OF NECROPSY: 01/20/00 10:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK EYE (EY) : 75; 60 X 50MM; VERIFIED AT NECROPSY: -INFLAMMATION, ACUTE,-MODERATE OBSERVED -ULCERATION, CORNEAL,-MODERATE >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >EXCRETION; FEW FECES; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: NOT APPLICABLE -DIFFUSELY DARK; ALL LOBES: DARK BROWN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >APPEARANCE; TREMORS; ENTIRE BODY; -HYPERTROPHY, HEPATOCELLULAR, VERIFIED AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; OCCASIONALLY NOTED,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE -GRANULAR CYTOPLASM, EOSINOPHILIC, >BEHAVIOR; HYPOACTIVE; VERIFIED AT CENTRILOBULAR,-MODERATE NECROPSY: NOT APPLICABLE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT -MINERALIZATION, TUBULAR,-MODERATE SPLEEN (SP) : SPLEEN (SP) : -SMALL; 24 X 5 X 3 MM -PIGMENT, INCREASED,-MODERATE 3857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2096 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93175 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS DATE AND TIME OF NECROPSY: 01/20/00 10:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE -CONGESTION,-PRESENT MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE -CONGESTION,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 6 X 4 MM MODERATE HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND FIRM 3858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2097 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93175 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS DATE AND TIME OF NECROPSY: 01/20/00 10:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 60 X 39 X 15 MM, DARK -CYST / GALACTOCELE,-PRESENT RED, TAN, LOBULATED AND FIRM; SAME ON -HYPERPLASIA, GLANDULAR,-SLIGHT CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE LIGHT GREEN MUCOID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DRY RED MATERIAL -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; ALONG ENTIRE LENGTH: DARK BROWN MATERIAL -------------------------------------------- 3859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2098 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93175 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS DATE AND TIME OF NECROPSY: 01/20/00 10:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2099 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93176 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 9:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 72; 30 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-RIGHT; WEEK LIVER (LI) : 79; 10 X 10MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -HYPERTROPHY, HEPATOCELLULAR, >SKIN & PELAGE; BLACK SKIN; VENTRAL- CENTRILOBULAR POLYKARYOCYTOSIS ABDOMINAL-LEFT; VERIFIED AT NECROPSY: OCCASIONALLY NOTED,-SLIGHT NOT OBSERVED -GRANULAR CYTOPLASM, EOSINOPHILIC, >APPEARANCE; THIN; VERIFIED AT CENTRILOBULAR,-SLIGHT NECROPSY: OBSERVED -PIGMENT, HEPATOCELLULAR, >APPEARANCE; HUNCHED; VERIFIED AT CENTRILOBULAR,-MINIMAL NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >EYES; RED DISCHARGE; EYES; VERIFIED AT EOSINOPHILIC CELL,-MINIMAL NECROPSY: OBSERVED -DEGENERATION, CYSTIC,-MINIMAL >SKIN & PELAGE; ROUGH HAIR COAT; -HYPERPLASIA, BILE DUCT,-SLIGHT VERIFIED AT NECROPSY: NOT APPLICABLE -FIBROSIS, PERIBILIARY,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >EXCRETION; NO FECES; VERIFIED AT -INFLAMMATION, SUPPURATIVE, PELVIC,- NECROPSY: NOT APPLICABLE MINIMAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED SPLEEN (SP) : AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT 3861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2100 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93176 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 9:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN, AND SOFT STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE TAN ERODED AREAS, UP TO 5 X 2 MM -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,- MODERATELY SEVERE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT 3862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2101 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93176 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 9:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL REGION: >NOTE:>MASS #1 IS THE CARCINOMA SINGLE MASS, 25 X 25 X 15 MM, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 15 X 15 X 10 MM, RED, DARK RED, TAN, AND SOFT; CUT SURFACE CONTAINS A DARK RED SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY -------------------------------------------- 3863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2102 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93176 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 9:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2103 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93177 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:28 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SURFACE OF RIGHT MEDIAN LOBE: MULTIPLE -HYPERTROPHY, HEPATOCELLULAR, DARK RED AREAS, UP TO 4 X 3 MM; CENTRILOBULAR POLYKARYOCYTOSIS COLLECTED ON ROUTINE SECTION OCCASIONALLY NOTED,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2104 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93177 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:28 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT -HYPERTROPHY, CORTICAL CELL,-MODERATELY IS 3 MM IN DIAMETER) SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-MINIMAL OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-MODERATE PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); DIFFUSE: -B-ADENOMA,-NEOPLASM PRESENT MULTIPLE BLACK AREAS, UP TO 3 X 2 MM MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -------------------------------------------- 3866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93177 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:28 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2106 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93178 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:49 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYE-LEFT; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : SPLEEN (SP) : -SMALL; 34 X 6 X 4 MM -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 3868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2107 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93178 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:49 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 5 X 6 X 7 MM, APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -DARK FOCUS(I)/AREA(S); ANTERIOR ASPECT: MULTIPLE BLACK AREAS, UP TO 3 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-MODERATE AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, DRY, CRUSTED MATERIAL -------------------------------------------- 3869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2108 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93178 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:49 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2109 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 95; 40 X 40MM; VERIFIED AT NECROPSY: -LIGHT FOCUS(I)/AREA(S); JUNCTION OF -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT OBSERVED MEDIAN LOBE: SINGLE TAN AREA, 3 X 2 MM -HYPERTROPHY, HEPATOCELLULAR, >SKIN & PELAGE; SORE/SCAB; AXILLARY (CAP 22) CENTRILOBULAR POLYKARYOCYTOSIS REGION-LEFT; VERIFIED AT NECROPSY: OCCASIONALLY NOTED,-SLIGHT OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 3871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2110 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2111 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 40 X 25 X 20 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS MATERIAL; OVERLYING SKIN HAS A SINGLE MOIST TAN AND ERODED AREA, 8 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) UTERUS (UT) : UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- CLEAR FLUID FILLED CYST, 10 X 5 X 5 MM MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: 10 X 7 X 2 MM (RIGHT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,- 5 X 2 X 1 MM) (CAP 23) MODERATELY SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2112 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2113 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93180 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 578.3 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 56; 110 X 80MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE OBSERVED LIVER (LI) : LIVER (LI) : -LARGE; ALL LOBES: THICKENED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 3875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2114 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93180 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 578.3 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE LIGHT AREAS, UP TO 4 X 3 MM SLIGHT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 7 X 7 X 5 MM; APPEARS SWOLLEN -B-ADENOMA,-NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY, EXTENDING TO -CYST / GALACTOCELE,-PRESENT LEFT ABDOMINAL REGION: SINGLE MASS, >NOTE:>SEE SKIN FOR DIAGNOSIS 130 X 80 X 70 MM (277.8 GRAMS), RED, TAN AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,- PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2115 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93180 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 578.3 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2116 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93181 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID >UNREMARKABLE FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : CERVIX (CV) : -MASS(ES); WITHIN LUMEN, EXTENDING -B-POLYP, STROMAL,-NEOPLASM PRESENT INTO VAGINA: SINGLE MASS, 9 X 5 X 5 MM, DARK RED, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN 3878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2117 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93181 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) ------------------------------------------------------------------------------------------------------------------------------------ 3879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2118 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93182 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 102; 15 X 15MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / SURFACE OF RIGHT MEDIAN LOBE: SINGLE EOSINOPHILIC CELL,-MINIMAL DARK RED FOCUS, 5 MM IN DIAMETER; -CYST, BILIARY,-PRESENT COLLECTED ON ROUTINE SECTION -CYST(S); LEFT LATERAL LOBE ALONG MARGIN: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT STOMACH, GL (ST) : >UNREMARKABLE 3880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2119 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93182 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, ALONG GREATER CURVATURE: SINGLE BLACK AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 19 X 19 X 10 MM, RED, -B-FIBROADENOMA,-NEOPLASM PRESENT DARK RED, GRAY, TAN AND SOFT; SAME ON -HYPERPLASIA, GLANDULAR,-MODERATE CUT SURFACE (CAP 22); REGIONAL LN >NOTE:>MASS #2 IS THE FIBROADENOMA (AXILLARY) NORMAL (CAP 24) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 33 X 21 X 15 MM, RED, GRAY, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2120 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93182 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: OVARY (OV), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2121 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93183 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 772.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 91; 125 X 100MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 4 -DEGENERATION, CYSTIC / HEMORRHAGIC,- MM IN DIAMETER) MODERATELY SEVERE -PIGMENT,-MINIMAL SKIN (SK) : SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL >NOT REQUIRED TO BE EXAMINED FOR ANIMAL REGION DUE TO MF MASS MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2122 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93183 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 772.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS(ES); LEFT ABDOMINAL REGION EXTENDING TO LEFT INGUINAL REGION: SINGLE MASS, 150 X 100 X 50 MM (327.7 GRAMS), RED, DARK RED, TAN, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; PORTIONS COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED, DRY CRUSTED AREA, 30 X 15 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MF MASS UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2123 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93183 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 772.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2124 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93184 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 526.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:28 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >WART LIKE LESION(S); MOUTH; WEEK 102; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE LIVER (LI) : LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE RED AREAS, UP TO 4 X 3 MM; -VACUOLATION, HEPATOCELLULAR, COLLECTED ON ROUTINE SECTIONS PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 12 MM IN DIAMETER LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, GRAY, TAN AND SOFT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 3886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2125 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93184 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 526.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:28 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN, SLIGHTLY ERODED AREAS, UP TO 2 X 1 MM SKIN (SK) : SKIN (SK) : -RAISED FOCUS(I)/AREA(S); ATTACHED TO -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT LOWER RIGHT LIP: SINGLE TAN AREA, 5 X -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM 4 X 3 MM (CAP 22) PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -DILATATION,-MODERATELY SEVERE MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2126 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93184 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 526.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:28 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2127 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93185 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 521.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:51 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT EYE (EY) : EYE (EY) : NECROPSY: OBSERVED -OPAQUE CORNEA; LEFT -DYSTROPHY, CORNEAL IN EARLY STAGES, >EYES; OPAQUE; EYE-LEFT; VERIFIED AT CONSISTS PRIMARILY OF FOCAL NECROPSY: OBSERVED MINERALIZATION,-SLIGHT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -HYPERPLASIA, GRANULOSA CELL,-MODERATE FILLED CYST, 6 X 3 X 3 MM LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : PARATHYROID (PT) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 1 >UNREMARKABLE MM IN DIAMETER) MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2128 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93185 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 521.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:51 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -HEMORRHAGE,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,- MODERATE -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; LEFT: DARK RED, DRY MATERIAL -------------------------------------------- 3890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2129 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93185 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 521.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:51 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) ------------------------------------------------------------------------------------------------------------------------------------ 3891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2130 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93186 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 458.9 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 14:40 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-CERVICAL; WEEK 45; LIVER (LI) : 100 X 110MM; VERIFIED AT NECROPSY: -FOCUS, ALTERED HEPATOCELLULAR, OBSERVED BASOPHILIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2131 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93186 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 458.9 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 14:40 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -CYST(S); RIGHT ASPECT: TWO DARK RED FLUID FILLED CYSTS, ONE IS 2 MM IN DIAMETER, THE OTHER IS 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION, -CYST / GALACTOCELE,-PRESENT EXTENDS TO LEFT AXILLARY AND VENTRAL -B-FIBROADENOMA,-NEOPLASM PRESENT CERVICAL REGIONS: SINGLE MASS, 115 X 90 X 35 MM (187.9 GRAMS), RED, TAN, LOBULATED AND VASCULAR; SAME ON CUT SURFACE (PORTIONS COLLECTED); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS, UP TO 10 X 6 X 5 MM (CAP 21) UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2132 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93186 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 458.9 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 14:40 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2133 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93187 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/00 STUDY DAY OF DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS DATE AND TIME OF NECROPSY: 04/13/00 14:27 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 81; 65 X 60MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 102; 10 X 10MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / >EXCRETION; FEW FECES; VERIFIED AT EOSINOPHILIC CELL,-SLIGHT NECROPSY: NOT APPLICABLE -FOCUS, ALTERED HEPATOCELLULAR, >APPEARANCE; MALOCCLUSION; VERIFIED AT BASOPHILIC,-SLIGHT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL >BEHAVIOR; ATAXIC; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; ROUGH HAIR COAT; MINIMAL VERIFIED AT NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-SLIGHT >BEHAVIOR; HYPOACTIVE; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-SLIGHT >SKIN & PELAGE; PALE; ENTIRE BODY; -HEMATOPOIESIS, EXTRAMEDULLARY, VERIFIED AT NECROPSY: NOT APPLICABLE INCREASED,-SLIGHT >APPEARANCE; HEAD TILT; LEFT; VERIFIED ADRENAL, CORTEX (AC) : AT NECROPSY: NOT APPLICABLE -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- SLIGHT OVARY (OV) : -CYST,-PRESENT 3895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2134 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93187 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/00 STUDY DAY OF DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS DATE AND TIME OF NECROPSY: 04/13/00 14:27 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 8 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,- MODERATE SKIN (SK) : >NOTE:>SKIN AND MAMMARY GLAND SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, -B-FIBROADENOMA,-NEOPLASM PRESENT EXTENDING TO MID ABDOMINAL REGION: -B-ADENOMA,-NEOPLASM PRESENT SINGLE MASS, 70 X 40 X 30 MM, DARK >NOTE:>MASS #1 IS THE FIBROADENOMA RED, TAN, VASCULAR AND FIRM; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 22) 3896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2135 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93187 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/00 STUDY DAY OF DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS DATE AND TIME OF NECROPSY: 04/13/00 14:27 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 15 MM IN DIAMETER, GREEN, BROWN AND SOFT; ON SECTION EXUDES A BROWN SEMIFLUID; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 23) UTERUS (UT) : UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MULTIPLE CLEAR FLUID FILLED CYSTS, UP -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- TO 7 MM IN DIAMETER MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -------------------------------------------- 3897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2136 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93187 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/00 STUDY DAY OF DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS DATE AND TIME OF NECROPSY: 04/13/00 14:27 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2137 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK LIVER (LI) : LIVER (LI) : 70; 100 X 100MM; VERIFIED AT NECROPSY: -LARGE; ALL LOBES: MARGINS APPEAR -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED ROUNDED -NECROSIS, COAGULATIVE,-SLIGHT >SKIN & PELAGE; SORE/SCAB; AXILLARY -DIFFUSELY LIGHT; ALL LOBES: TAN -HYPERTROPHY, HEPATOCELLULAR, REGION-LEFT; VERIFIED AT NECROPSY: PERIPORTAL,-MODERATE OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2138 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: >UNREMARKABLE MULTIPLE BROWN AREAS, UP TO 2 MM IN DIAMETER CECUM (CE) : >AUTOLYTIC SKIN (SK) : >NOTE:>SKIN TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION -CYST / GALACTOCELE,-PRESENT EXTENDING TO LEFT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 130 X 100 X 65 MM (292.7 -HYPERPLASIA, GLANDULAR,-MODERATE GRAMS), RED, DARK RED, TAN, GRAY, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 25 X 16 MM; COLLECTED INTACT WITH MASS >NOTE:>MAMMARY SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 3900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2139 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 10 X 7 X 3 MM (RIGHT IS UP TO 6 X 3 X 2 MM) (CAP 21) -MOTTLED; RIGHT: DARK RED AND TAN -DIFFUSELY RED; LEFT GENERAL COMMENT (GC) : -ANIMAL OBESE -------------------------------------------- 3901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2140 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2141 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93189 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 220.8 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 13:47 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >APPEARANCE; THIN; VERIFIED AT LIVER (LI) : NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, MACROPHAGE, PIGMENTED,- NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT KIDNEY (KD) : NECROPSY: NOT APPLICABLE -MINERALIZATION, PELVIC,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT SPLEEN (SP) : NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE >APPEARANCE; HEAD TILT; LEFT; VERIFIED -HEMATOPOIESIS, EXTRAMEDULLARY, AT NECROPSY: NOT APPLICABLE INCREASED,-MINIMAL PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,- MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 11 X 10 MM, RED, GREY -B-ADENOMA,-NEOPLASM PRESENT AND SOFT 3903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2142 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93189 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 220.8 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 13:47 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EROSION,-MINIMAL DARK BROWN FOCUS, 3 X 2 MM MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -HYPERPLASIA, MUCINOUS,-SLIGHT LIGHT GREEN, MUCOID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -ANIMAL THIN -------------------------------------------- 3904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2143 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93189 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 220.8 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 13:47 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2144 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93190 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 280.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:56 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >SKIN & PELAGE; ROUGH HAIR COAT; -COMPRESSION, VENTRAL,-PRESENT VERIFIED AT NECROPSY: NOT APPLICABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,- MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GREY, AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE -EROSION,-MINIMAL BLACK LINEAR AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2145 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93190 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 280.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:56 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT) ------------------------------------------------------------------------------------------------------------------------------------ 3907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2146 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93191 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 539.2 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:19 PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >EYES; RED DISCHARGE; EYES; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >RESPIRATION; AUDIBLE; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : LIVER (LI) : >RESPIRATION; LABORED; VERIFIED AT -HEPATIC ANOMALY (LOBULE); BETWEEN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE MEDIAN LOBES, NEAR HILUS: SINGLE -VACUOLATION, HEPATOCELLULAR, MIDZONAL >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED ANOMALY, 10 X 10 X 6 MM; COLLECTED / CENTRILOBULAR,-SLIGHT AT NECROPSY: NOT APPLICABLE -ANOMALY, SECONDARY TO DIAPHRAGMATIC >BEHAVIOR; HYPOACTIVE; VERIFIED AT HERNIA,-PRESENT NECROPSY: NOT APPLICABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2147 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93191 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 539.2 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:19 PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 6 MM, DARK RED AND -B-ADENOMA,-NEOPLASM PRESENT SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -CYST / GALACTOCELE,-PRESENT AND FIRM; COLLECTED ON ROUTINE SECTION -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -DILATATION,-MINIMAL CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: CLEAR AND MOIST -------------------------------------------- 3909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2148 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93191 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 539.2 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:19 PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2149 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93192 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 519.3 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : LIVER (LI) : WEEK 91; 70 X 75MM; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT NECROPSY: OBSERVED MULTIPLE DARK RED AREAS, UP TO 3 X 2 -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / MM; COLLECTED ON ROUTINE SECTIONS EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -CYST, BILIARY,-PRESENT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT THYMUS (TH) : THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT -CYST,-PRESENT POSITIVELY IDENTIFIED; POSSIBLE -HYPERPLASIA, EPITHELIAL,-MODERATE REMNANTS COLLECTED THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 6 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT 3911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2150 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93192 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 519.3 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 75 X 60 X 30 MM, TAN, -B-FIBROADENOMA,-NEOPLASM PRESENT LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2151 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93192 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 519.3 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2152 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93193 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 420.9 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:28 PROSECTOR: LYNN HORNER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; RED HAIR COAT; HEAD- BRAIN (BR) : BRAIN (BR) : ENTIRE; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >SKIN & PELAGE; YELLOW HAIR COAT; LIVER (LI) : VERIFIED AT NECROPSY: NOT OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >BEHAVIOR; HYPOACTIVE; VERIFIED AT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / NECROPSY: NOT APPLICABLE EOSINOPHILIC CELL,-MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -VACUOLATION, HEPATOCELLULAR,-SEVERE NECROPSY: NOT APPLICABLE KIDNEY (KD) : >EXCRETION; NONFORMED FECES; VERIFIED -NEPHROPATHY, CHRONIC PROGRESSIVE,- AT NECROPSY: NOT APPLICABLE MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); LEFT: MULTIPLE -ANGIECTASIS,-SLIGHT DARK BROWN AREAS, UP TO 2 MM IN -PIGMENT,-MINIMAL DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2153 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93193 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 420.9 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:28 PROSECTOR: LYNN HORNER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 6 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: -CYST / GALACTOCELE,-PRESENT MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 21) CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED AND DRY -------------------------------------------- 3915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2154 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93193 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 420.9 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:28 PROSECTOR: LYNN HORNER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2155 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93194 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 13:51 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; ROUGH HAIR COAT; EYE (EY) : VERIFIED AT NECROPSY: NOT APPLICABLE -INFLAMMATION, ACUTE,-SLIGHT >SKIN & PELAGE; SORE/SCAB; VENTRAL- LIVER (LI) : THORACIC; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK BASOPHILIC,-MINIMAL 82; 10 X 10MM; VERIFIED AT NECROPSY: KIDNEY (KD) : NOT OBSERVED -MINERALIZATION, TUBULAR,-MINIMAL >SKIN & PELAGE; ALOPECIA; VERIFIED AT SPLEEN (SP) : NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, >SKIN & PELAGE; SORE/SCAB; VENTRAL- INCREASED,-SLIGHT ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -HYPERPLASIA, LYMPHORETICULAR,-MODERATE OBSERVED PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -HYPERPLASIA, CORTICAL,-MINIMAL OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2156 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93194 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 13:51 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED -B-ADENOMA,-NEOPLASM PRESENT AND SOFT HARDERIAN GLAND (HG) : -ATROPHY,-SLIGHT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); ALL REGIONS DIFFUSE: -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT MULTIPLE TAN DRY CRUSTED AREAS, UP TO -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 25 X 7 MM; COLLECTED ON ROUTINE -ULCERATION,-SLIGHT SECTION -ALOPECIA-DIFFUSE; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2157 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93194 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 13:51 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 20 X 15 X 5 -HEMORRHAGE,-PRESENT MM, RIGHT IS 13 X 10 X 4 MM (CAP 21) -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE -DIFFUSELY RED; RIGHT: DARK RED -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -FIBROPLASIA, PERIPHERAL,-SEVERE LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE -LARGE; BILATERAL: UP TO 15 X 10 X 4 -HEMORRHAGE,-PRESENT MM; COLLECTED -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 15 X 10 X 4 MM (RIGHT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 6 X 5 X 3 MM) (CAP 22) -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2158 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93194 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 13:51 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2159 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93195 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/23/99 11:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-MINIMAL THYMUS (TH) : THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: -HEMORRHAGE,-PRESENT MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT 3921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2160 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93195 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/23/99 11:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT TWO MASSES, ONE IS 15 X 12 X 6 MM, THE OTHER IS 11 X 7 X 6 MM, BOTH ARE TAN AND SOFT; SAME ON BOTH CUT SURFACES; THE LARGER MASS EXUDES A TAN CASEOUS SEMISOLID MATERIAL; BOTH COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 3922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2161 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93195 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/23/99 11:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2162 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93196 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 304.7 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 8:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -HEMORRHAGE,-MINIMAL NECROPSY: NOT APPLICABLE LIVER (LI) : >EYES; RED DISCHARGE; EYES; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: OBSERVED -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,- SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2163 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93196 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 304.7 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 8:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT CERVIX (CV) : -HYPERPLASIA, STROMAL,-MINIMAL VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT CONTAINS A DARK RED SEMIFLUID CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -------------------------------------------- 3925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2164 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93196 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 304.7 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 8:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2165 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93197 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 334.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAW-HIND- BRAIN (BR) : BRAIN (BR) : LEFT; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) >UNREMARKABLE >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 93; 20 X 20MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -VACUOLATION, HEPATOCELLULAR, >MASS(ES); INGUINAL REGION-LEFT; WEEK PERIPORTAL,-SLIGHT 95; 40 X 40MM; VERIFIED AT NECROPSY: -NECROSIS, COAGULATIVE,-SLIGHT OBSERVED -HYPERPLASIA, BILE DUCT,-MINIMAL >MASS(ES); AXILLARY REGION-LEFT; WEEK -HYPERTROPHY, HEPATOCELLULAR, 99; 10 X 10MM; VERIFIED AT NECROPSY: PERIPORTAL,-MODERATE OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 12 X 8 X 6 MM LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 6 X 3 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT SKIN (SK) : SKIN (SK) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT >NOT REQUIRED TO BE EXAMINED FOR ANIMAL INGUINAL REGION DUE TO MASS 3927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2166 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93197 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 334.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 215 X 10 X 4 MM, RED, -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT DARK RED, AND SOFT; SAME ON CUT -B-FIBROADENOMA,-NEOPLASM PRESENT SURFACE; SOME SECTIONS EXUDE A BROWN >NOTE:>MASS #2 IS THE FIBROADENOMA MASS SEMISOLID (CAP 23); REGIONAL LN #1 CONSISTS OF GALACTOCELES (AXILLARY) NORMAL (CAP 24) -MASS #2; LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 45 X 35 X 25 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE HINDFOOT: SINGLE MOIST AND RED AREA, 6 -ULCERATION,-MODERATE X 5 MM; COLLECTED -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2167 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93197 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 334.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2168 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93198 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 555.3 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 94; 10 X 10MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- MODERATE PITUITARY (PI) : PITUITARY (PI) : -CYST(S); LEFT AND RIGHT POSTERIOR -B-ADENOMA,-NEOPLASM PRESENT ASPECTS: TWO CLEAR FLUID FILLED CYSTS, EACH IS 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 27 X 27 X 10 MM, TAN, -HYPERPLASIA, GLANDULAR,-MINIMAL WHITE AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2169 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93198 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 555.3 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) ------------------------------------------------------------------------------------------------------------------------------------ 3931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2170 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93199 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 319.8 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 8:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; HEAD TILT; RIGHT; VERIFIED -COMPRESSION, VENTRAL,-PRESENT AT NECROPSY: NOT APPLICABLE LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2171 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93199 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 319.8 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 8:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : -MASS(ES); 12 X 10 X 10 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2172 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93199 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 319.8 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 8:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2173 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93200 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 8:06 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; YELLOW HAIR COAT; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EYES; RED DISCHARGE; EYES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE MULTIPLE DARK RED AREAS, UP TO 3 X 2 -VACUOLATION, HEPATOCELLULAR, >EXCRETION; NO FECES; VERIFIED AT MM; COLLECTED ON ROUTINE SECTIONS PERIPORTAL,-MODERATELY SEVERE NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >APPEARANCE; THIN; VERIFIED AT MINIMAL NECROPSY: OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,- MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MODERATE 3935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2174 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93200 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 8:06 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 8 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT DARK RED, GRAY, TAN AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,- MODERATELY SEVERE MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN -HYPERPLASIA, GLANDULAR,-MODERATE AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: -DILATATION,-SEVERE CLEAR FLUID -LARGE, DIFFUSE; BILATERAL: EACH HORN IS 50 X 13 X 10 MM 3936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2175 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93200 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 8:06 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -------------------------------------------- 3937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2176 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93200 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/19/00 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS DATE AND TIME OF NECROPSY: 02/19/00 8:06 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2177 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93201 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 434.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:40 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MULTIPLE DARK RED AREAS, UP TO 4 MM IN -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / DIAMETER; COLLECTED ON ROUTINE EOSINOPHILIC CELL,-SLIGHT SECTIONS -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR -HYPERPLASIA, GRANULOSA CELL,-SEVERE FLUID FILLED CYSTS ON EACH, UP TO 1 MM IN DIAMETER THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: -B-ADENOMA,-NEOPLASM PRESENT SINGLE DARK RED AREA, 2 MM IN DIAMETER -HYPERPLASIA,-MINIMAL -CYST,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT COLLECTED -HEMORRHAGE,-PRESENT 3939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2178 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93201 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 434.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:40 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2179 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93202 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 436.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:38 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,- MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, MACROPHAGE, ALVEOLAR,- MULTIPLE TAN AREAS, UP TO 6 X 4 MM MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2180 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93202 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 436.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:38 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2181 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 76; 15 X 25MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; VENTRAL- -DIFFUSELY LIGHT; ALL LOBES: TAN -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ABDOMINAL-LEFT; VERIFIED AT NECROPSY: -VACUOLATION, HEPATOCELLULAR, OBSERVED PERIPORTAL,-MINIMAL >SKIN & PELAGE; SORE/SCAB; VENTRAL- -FOCUS, ALTERED HEPATOCELLULAR, ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: BASOPHILIC,-MINIMAL OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT >MASS(ES); INGUINAL REGION-LEFT; WEEK -HYPERTROPHY, HEPATOCELLULAR, 81; 25 X 30MM; VERIFIED AT NECROPSY: PERIPORTAL,-MINIMAL OBSERVED KIDNEY (KD) : KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,- MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE 3943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2182 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 4 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 15 X 15 X 10 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 3 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -B-ADENOMA,-NEOPLASM PRESENT 3944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2183 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: >NOTE:>THE ADENOMA WAS DESCRIBED AS A SINGLE MASS, 20 X 10 X 10 MM, DARK CYST MASS #2 IS A FIBROADENOMA RED, BROWN AND SOFT; CUT SECTION MASSES 1,3, AND 4 ARE CARCINOMA CONTAINS A DARK RED FLUID; COLLECTED; MASS 4 ALSO HAS AREAS WITH REGIONAL LN (SEE AXILLARY) FEATURES OF FIBROADENOMA -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 30 X 30 X 20 MM, TAN, DARK RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -CYST(S); BILATERAL PECTORAL REGION: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 22) -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 10 MM, BROWN, TAN, AND SOFT; CUT SURFACE CONTAINS A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 60 X 60 X 40 MM, DARK RED, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS TWO DARK RED, TAN, AND MOIST ABRADED AREAS, BOTH ARE 15 MM IN DIAMETER; COLLECTED INTACT; POSSIBLY CONSISTENT WITH SORE/SCAB VENTRAL ABDOMINAL LEFT AND RIGHT NOTED IN CLINICAL OBSERVATIONS 3945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2184 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -HYPERTROPHY, ENDOMETRIUM,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS UP TO 20 X -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 11 X 7 MM, RIGHT IS UP TO 12 X 10 X 5 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2185 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2186 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93204 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/21/00 7:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; LIVER (LI) : WEEK 66; 70 X 80MM; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT NECROPSY: OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,- MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2187 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93204 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/21/00 7:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -B-ADENOMA,-NEOPLASM PRESENT -RED FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED AREA, 3 X 2 MM STOMACH, NONGL (SU) : STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE >UNREMARKABLE MOIST TAN ERODED AREA, 3 MM IN DIAMETER JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -B-FIBROADENOMA,-NEOPLASM PRESENT SINGLE MASS, 90 X 89 X 34 MM, RED, -HYPERPLASIA, GLANDULAR,-MODERATE TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2188 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93204 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/21/00 7:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE -HEMORRHAGE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); NEAR RIGHT OVARY, ATTACHED -INFLAMMATION / NECROSIS, FAT,- TO ADIPOSE TISSUE: SINGLE MASS, 25 X MODERATELY SEVERE 12 X 10 MM, TAN, GREEN, RED AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (LUMBAR) NORMAL (CAP 22) -------------------------------------------- 3950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2189 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93204 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/21/00 7:49 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2190 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93205 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 428.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -I-CARCINOMA,-FROM PITUITARY LIVER (LI) : LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); VISCERAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SURFACE OF LEFT LATERAL LOBE: SINGLE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / TAN AREA, 4 X 3 MM; COLLECTED ON EOSINOPHILIC CELL,-MODERATE ROUTINE SECTION -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- SLIGHT OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 11 MM IN DIAMETER THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 5 X 2 MM, RED, DARK -M-CARCINOMA,-NEOPLASM PRESENT RED, GRAY AND SOFT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2191 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93205 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 428.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -HEMORRHAGE,-PRESENT (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2192 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93206 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 236.5 GRAMS DATE AND TIME OF NECROPSY: 04/15/00 7:50 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; THIN; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >EXCRETION; FEW FECES; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE -INFILTRATE, MACROPHAGE, PIGMENTED,- >EYES; RED DISCHARGE; EYES; VERIFIED AT MINIMAL NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -VACUOLATION, HEPATOCELLULAR, NECROPSY: NOT APPLICABLE PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,- MINIMAL -PIGMENT,-MINIMAL 3954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2193 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93206 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 236.5 GRAMS DATE AND TIME OF NECROPSY: 04/15/00 7:50 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, TAN, DARK -B-ADENOMA,-NEOPLASM PRESENT RED AND SOFT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN -------------------------------------------- 3955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2194 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93206 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 236.5 GRAMS DATE AND TIME OF NECROPSY: 04/15/00 7:50 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2195 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93207 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 281 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 204.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 14:02 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK LIVER (LI) : LIVER (LI) : 10; 55 X 55MM; VERIFIED AT NECROPSY: -DIFFUSELY DARK; ALL LOBES: DARK BROWN -INFILTRATE, MACROPHAGE, PIGMENTED,- OBSERVED SLIGHT >APPEARANCE; THIN; VERIFIED AT KIDNEY (KD) : KIDNEY (KD) : NECROPSY: OBSERVED -DIFFUSELY DARK; BILATERAL: DARK BROWN -MINERALIZATION, TUBULAR,-MODERATE >APPEARANCE; HUNCHED; VERIFIED AT -MASS(ES); RIGHT, CORTICAL SURFACE, -N-CARCINOMA,-FROM MAMMARY, FEMALE NECROPSY: NOT APPLICABLE EXTENDING TO MEDULLA: MULTIPLE MASSES, >EYES; RED DISCHARGE; EYES; VERIFIED AT UP TO 8 X 5 X 3 MM, TAN AND FIRM; SAME NECROPSY: OBSERVED ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (RENAL) NOT IDENTIFIED SPLEEN (SP) : SPLEEN (SP) : -SMALL; 29 X 5 X 3 MM -PIGMENT, INCREASED,-MODERATE -DEPLETION, LYMPHOCYTIC,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : -N-CARCINOMA,-FROM MAMMARY, FEMALE 3957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2196 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93207 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 281 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 204.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 14:02 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP TO 15 X 10 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED STOMACH, NONGL (SU) : -EDEMA,-PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: -M-CARCINOMA,-NEOPLASM PRESENT SINGLE MASS, 70 X 40 X 30 MM, RED, DARK RED, TAN, BROWN, SOFT, AND LOBULATED; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK BROWN, TENACIOUS SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK BROWN, RED, -HEMORRHAGE,-PRESENT AND TAN (CAP 20) -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: DISTENDED WITH GAS 3958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2197 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93207 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 281 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 204.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 14:02 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2198 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93208 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 6:55 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >RESPIRATION; AUDIBLE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >RESPIRATION; IRREGULAR; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT APPLICABLE LIVER (LI) : >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED -HYPERTROPHY, HEPATOCELLULAR, AT NECROPSY: NOT APPLICABLE CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -SMALL; LEFT: 3 X 2 X 2 MM (RIGHT IS 5 -ANGIECTASIS,-SLIGHT X 4 X 4 MM) -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE OVARY (OV) : OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR -CYST,-PRESENT FLUID FILLED CYST ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 3 MM IN DIAMETER SPINAL CORD (SC) : -HEMORRHAGE,-SLIGHT 3960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2199 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93208 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 6:55 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MINIMAL LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 7 X 5 MM, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, LOBULATED, AND SOFT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: -LUMEN, EXUDATE,-PRESENT LIGHT GREEN CASEOUS SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT -------------------------------------------- 3961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2200 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93208 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 6:55 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2201 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93209 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 604.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : THYROID (TY) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 -HYPERPLASIA, C-CELL,-SLIGHT X 3 X 2 MM) -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -DIFFUSELY RED; LEFT: DARK RED JEJUNUM (JE) : -M-CARCINOMA,-NEOPLASM PRESENT 3963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2202 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93209 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 604.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ JEJUNUM (JE) : -MASS(ES); WITHIN WALL: SINGLE MASS, 17 X 11 X 10 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2203 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93209 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 604.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2204 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-RIGHT; WEEK BRAIN (BR) : BRAIN (BR) : 88; 30 X 35MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT OBSERVED -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); INGUINAL REGION-LEFT; WEEK LIVER (LI) : 94; 50 X 50MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED KIDNEY (KD) : >SKIN & PELAGE; YELLOW HAIR COAT; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: OBSERVED MINIMAL >EXCRETION; FEW FECES; VERIFIED AT -MINERALIZATION, PELVIC,-MINIMAL NECROPSY: NOT APPLICABLE SPLEEN (SP) : >APPEARANCE; THIN; VERIFIED AT -PIGMENT, INCREASED,-MODERATE NECROPSY: OBSERVED -HEMATOPOIESIS, EXTRAMEDULLARY, >BEHAVIOR; HYPOACTIVE; VERIFIED AT INCREASED,-MINIMAL NECROPSY: NOT APPLICABLE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL 3966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2205 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN AND SOFT SKIN (SK) : >NOTE:>SKIN TAKEN FROM RIGHT INGUINAL REGION DUE TO LARGER MASS ON LEFT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 35 X 26 X 17 MM, TAN, -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT RED, VASCULAR AND FIRM; SAME ON CUT -HYPERPLASIA, GLANDULAR,-MINIMAL SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 58 X 48 X 33 MM, RED, TAN AND SOFT; ON CUT SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED 3967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2206 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : >NOTE:>MAMMARY SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO LARGER MASS ON LEFT VAGINA (VA) : VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: >UNREMARKABLE GREEN MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: YELLOW -------------------------------------------- 3968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2207 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2208 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93211 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 339.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); INGUINAL REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 104; VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL THYMUS (TH) : THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: -HYPERPLASIA, LYMPHORETICULAR,-SEVERE SINGLE MASS, 11 X 10 X 4 MM, RED AND FIRM; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) LUNG (LU) : -MINERALIZATION, ALVEOLAR LUMINA,- MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 10 X 6 X 3 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT 3970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2209 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93211 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 339.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: -HYPERPLASIA, GLANDULAR,-SLIGHT SINGLE MASS, 16 X 12 X 8 MM, TAN AND -B-ADENOMA,-NEOPLASM PRESENT FIRM; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- -MASS(ES); ATTACHED TO WALL OF RIGHT MODERATE HORN, 8 MM FROM BIFURCATION: SINGLE -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT MASS, 20 X 20 X 15 MM, RED, TAN, VASCULAR AND CYSTIC; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : LN, MEDIASTINAL (ML) : -DIFFUSELY RED; (CAP 22) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2210 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93211 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 339.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2211 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93212 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/24/99 8:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; BRAIN (BR) : BRAIN (BR) : VERIFIED AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 4 MM -DEGENERATION, CYSTIC / HEMORRHAGIC,- -SMALL; RIGHT: 3 X 2 X 2 MM SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); RIGHT: SINGLE RED FLUID >UNREMARKABLE FILLED CYST, 8 MM IN DIAMETER 3973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2212 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93212 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/24/99 8:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 7 X 6 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT TAN AND SOFT STOMACH, GL (ST) : STOMACH, GL (ST) : -RAISED AREA(S); MUCOSA, NEAR JUNCTION >UNREMARKABLE OF NONGLANDULAR JUNCTION: SINGLE TAN AREA, 2 X 2 X 1 MM MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: -INFLAMMATION, CHRONIC-ACTIVE,- SINGLE DARK RED AND SLIGHTLY RAISED MODERATELY SEVERE AREA, 7 X 5 X 2 MM; COLLECTED; RIGHT -ACANTHOSIS / HYPERKERATOSIS,- NOT IDENTIFIED MODERATELY SEVERE LN, POPLITEAL (PP) : LN, POPLITEAL (PP) : -LARGE; LEFT: 13 X 8 X 4 MM (CAP 21); -HYPERPLASIA, PLASMACYTIC,-SEVERE RIGHT NOT IDENTIFIED -DIFFUSELY RED; LEFT: DARK RED 3974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2213 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93212 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/24/99 8:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ GENERAL INFORMATION (XX) : >NOTE:>TISSUE ON SLIDE #22 APPEARS TO BE A NECROTIC FIBROADENOMA. AS THERE WAS NO GROSS DESCRIPTION OF A LARGE SUBCUTANEOUS TUMOR, THE TISSUE CANNOT BE DEFINITIVELY ASSOCIATED WITH THIS ANIMAL. -------------------------------------------- 3975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2214 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93212 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/24/99 8:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2215 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93213 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 586 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 265.3 GRAMS DATE AND TIME OF NECROPSY: 11/26/99 13:11 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: NOT OBSERVED LIVER (LI) : >BEHAVIOR; HYPOACTIVE; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE -NECROSIS, INDIVIDUAL HEPATOCYTE,- >BEHAVIOR; ATAXIC; VERIFIED AT MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : >APPEARANCE; TREMORS; ENTIRE BODY; -NEPHROPATHY, CHRONIC PROGRESSIVE,- VERIFIED AT NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; HUNCHED; VERIFIED AT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,- NECROPSY: NOT APPLICABLE MINIMAL >APPEARANCE; HEAD TILT; LEFT; VERIFIED SPLEEN (SP) : AT NECROPSY: NOT APPLICABLE -PIGMENT, INCREASED,-MODERATE >EXCRETION; FEW FECES; VERIFIED AT -HEMATOPOIESIS, EXTRAMEDULLARY, NECROPSY: NOT APPLICABLE INCREASED,-MINIMAL >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : VERIFIED AT NECROPSY: NOT APPLICABLE -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,- >SKIN & PELAGE; PALE; ENTIRE BODY; IS 5 X 4 X 3 MM) MODERATELY SEVERE VERIFIED AT NECROPSY: NOT APPLICABLE -PIGMENT,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2216 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93213 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 586 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 265.3 GRAMS DATE AND TIME OF NECROPSY: 11/26/99 13:11 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, DARK RED, -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, GRAY AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -------------------------------------------- 3978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2217 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93213 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 586 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 265.3 GRAMS DATE AND TIME OF NECROPSY: 11/26/99 13:11 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2218 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93214 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/03/00 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/03/00 7:46 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT 3980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2219 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93214 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/03/00 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/03/00 7:46 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,- MODERATE PITUITARY (PI) : PITUITARY (PI) : -CYST(S); RIGHT ASPECT: SINGLE CYST, 3 -B-ADENOMA,-NEOPLASM PRESENT MM IN DIAMETER, FILLED WITH A DARK RED FLUID JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT URINARY BLADDER (UB) : -MINERALIZED MATERIAL, LUMEN,-PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) -HYPERPLASIA, LYMPHOPLASMACYTIC,- MODERATELY SEVERE -CONGESTION,-PRESENT -------------------------------------------- 3981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2220 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93214 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/03/00 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/03/00 7:46 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2221 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93215 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >BEHAVIOR; HYPOACTIVE; VERIFIED AT BRAIN (BR) : BRAIN (BR) : NECROPSY: NOT APPLICABLE -COMPRESSED (HYPOTHALAMUS) -DILATATION, VENTRICULAR,-PRESENT >APPEARANCE; THIN; VERIFIED AT -COMPRESSION, VENTRAL,-PRESENT NECROPSY: OBSERVED LIVER (LI) : >BEHAVIOR; ATAXIC; VERIFIED AT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NECROPSY: NOT APPLICABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : >UNREMARKABLE 3983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2222 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93215 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN, MUCOID SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -------------------------------------------- 3984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2223 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93215 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2224 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); EAR-RIGHT; WEEK 61; VERIFIED BRAIN (BR) : BRAIN (BR) : AT NECROPSY: OBSERVED -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT >MASS(ES); AXILLARY REGION-RIGHT; WEEK LIVER (LI) : 81; 50 X 60MM; VERIFIED AT NECROPSY: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED -FOCUS, ALTERED HEPATOCELLULAR, >MASS(ES); AXILLARY REGION-RIGHT; WEEK BASOPHILIC,-MINIMAL 93; 20 X 25MM; VERIFIED AT NECROPSY: -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL OBSERVED -HYPERPLASIA, BILE DUCT,-SLIGHT >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; -FIBROSIS, PERIBILIARY,-SLIGHT WEEK 96; 40 X 40MM; VERIFIED AT KIDNEY (KD) : NECROPSY: OBSERVED -NEPHROPATHY, CHRONIC PROGRESSIVE,- >SKIN & PELAGE; SORE/SCAB; VENTRAL- MINIMAL ABDOMINAL-RIGHT; VERIFIED AT NECROPSY: -MINERALIZATION, PELVIC,-MINIMAL OBSERVED ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK -LARGE; LEFT: 9 X 6 X 5 MM (RIGHT IS 6 >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 101; 25 X 25MM; VERIFIED AT NECROPSY: X 4 X 3 MM) OBSERVED LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,- MINIMAL PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 9 X 6 X 4 MM, RED, DARK -B-ADENOMA,-NEOPLASM PRESENT RED, TAN, AND SOFT -HYPERPLASIA,-MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2225 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SINGLE MASS, 70 X 60 X 30 MM, RED, -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT DARK RED, TAN, AND FIRM, SAME ON CUT -B-ADENOMA,-NEOPLASM PRESENT SURFACE; SOME SECTIONS EXUDE A TAN >NOTE:>MASS 4 IS THE ADENOMA CASEOUS SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT AXILLARY REGION, CAUDAL TO MASS #1: SINGLE MASS, 35 X 25 X 15 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (SEE AXILLARY) -MASS #3; LEFT PERIANAL REGION: SINGLE MASS, 40 X 30 X 15 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT INGUINAL REGION: SINGLE MASS, 50 X 30 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CREAMY SEMISOLID; OVERLYING SKIN HAS A SINGLE MOIST, BROWN, ULCERATED AREA, 20 X 15 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : UTERUS (UT) : -CYST(S); WITHIN LUMEN OF LEFT HORN: -B-POLYP, ENDOMETRIAL, STROMAL,- MULTIPLE CYSTS, UP TO 7 X 5 X 5 MM NEOPLASM PRESENT 3987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2226 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ UTERUS (UT) : UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF RIGHT HORN: -HYPERPLASIA, CYSTIC, ENDOMETRIAL,- SINGLE MASS, 10 X 6 X 4 MM, RED, DARK MODERATE RED, TAN, SOFT; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; RIGHT: 15 X 7 X 3 MM (LEFT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,- 5 X 3 X 1 MM) (CAP 25) MODERATELY SEVERE LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -LARGE; RIGHT: 10 X 5 X 2 MM (LEFT IS -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 5 X 3 X 1 MM) (CAP 23) LN, LUMBAR/ILIAC (LM) : LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID -LYMPHANGIECTASIS,-PRESENT FILLED CYST, 2 MM IN DIAMETER (CAP 24) -HYPERPLASIA, LYMPHOPLASMACYTIC,- -LARGE; RIGHT: 10 X 5 X 2 MM (LEFT IS MODERATELY SEVERE 7 X 3 X 1 MM) (CAP 24) PINNA (PN) : PINNA (PN) : -MASS(ES); RIGHT INVOLVING ENTIRE EAR -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM CANAL: SINGLE MASS, 12 X 10 X 6 MM, PRESENT RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE MANDIBULAR) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -------------------------------------------- 3988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2227 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2228 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93217 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 635 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 9:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >NORMAL; NO REMARKABLE OBSERVATIONS; LIVER (LI) : VERIFIED AT NECROPSY: OBSERVED -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : -CORPORA LUTEA, PERSISTENT,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT 3990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2229 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93217 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 635 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 9:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATE LUNG (LU) : LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: -MINERALIZATION, VASCULAR,-MINIMAL MULTIPLE TAN AREAS, UP TO 5 X 4 MM -INFILTRATE, MACROPHAGE, ALVEOLAR,- SLIGHT PITUITARY (PI) : PITUITARY (PI) : -RAISED AREA(S); LEFT MID ASPECT: -HYPERPLASIA,-SLIGHT SINGLE TAN AREA, 3 X 3 X 3 MM VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT -------------------------------------------- 3991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2230 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93217 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 635 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 9:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) ------------------------------------------------------------------------------------------------------------------------------------ 3992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2231 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93218 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 15:10 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >MASS(ES); AXILLARY REGION-LEFT; WEEK BRAIN (BR) : BRAIN (BR) : 50; 20 X 20MM; VERIFIED AT NECROPSY: -COMPRESSED (HYPOTHALAMUS) -COMPRESSION, VENTRAL,-PRESENT OBSERVED LIVER (LI) : LIVER (LI) : >SKIN & PELAGE; SORE/SCAB; AXILLARY -LARGE; ALL LOBES; MARGINS APPEAR -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO REGION-LEFT; VERIFIED AT NECROPSY: ROUNDED NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT OBSERVED -MOTTLED; ALL LOBES: BROWN AND TAN -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT KIDNEY (KD) : KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN -MINERALIZATION, PELVIC,-MINIMAL PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER SPLEEN (SP) : SPLEEN (SP) : -LARGE; 70 X 20 X 15 MM -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2232 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93218 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 15:10 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MARROW, FEMUR (FM) : -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,- MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,- MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),- MODERATELY SEVERE LUNG (LU) : LUNG (LU) : -RAISED FOCUS(I)/AREA(S); ALL LOBES: -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO MULTIPLE DARK RED AND TAN AREAS, UP TO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4 X 3 X 3 MM PITUITARY (PI) : PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED, TAN -B-ADENOMA,-NEOPLASM PRESENT AND SOFT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: -CYST / GALACTOCELE,-PRESENT SINGLE MASS, 55 X 25 X 15 MM, TAN, -HYPERPLASIA, GLANDULAR,-MINIMAL GRAY AND FIRM; SAME ON CUT SURFACE; >NOTE:>SEE SKIN FOR DIAGNOSIS OF TUMOR COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND TAN ABRADED AREA, 30 MM IN DIAMETER; COLLECTED INTACT 3994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2233 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93218 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 15:10 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, AXILLARY (AX) : LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 10 X 7 MM -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT (RIGHT IS UP TO 5 X 3 X 3 MM)(CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -------------------------------------------- 3995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2234 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93218 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 15:10 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV) ------------------------------------------------------------------------------------------------------------------------------------ 3996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2235 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93219 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 761.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:38 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >SKIN & PELAGE; SORE/SCAB; VENTRAL- LIVER (LI) : LIVER (LI) : THORACIC-RIGHT; VERIFIED AT NECROPSY: -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL OBSERVED MULTIPLE DARK RED AREAS, UP TO 5 X 4 -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / MM; COLLECTED ON ROUTINE SECTIONS EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -DILATATION, PELVIC,-SEVERE -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 5 MM (RIGHT IS 4 -HYPERTROPHY, CORTICAL CELL,-SEVERE X 3 X 2 MM) -DEGENERATION, CYSTIC / HEMORRHAGIC,- MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID -CYST,-PRESENT FILLED CYST, 10 MM IN DIAMETER THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE 3997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2236 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93219 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 761.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:38 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN FOCUS, LESS THAN 1 MM IN DIAMETER SKIN (SK) : SKIN (SK) : -CRUSTED AREA(S); RIGHT VENTRAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT ABDOMINAL REGION: SINGLE DRY, TAN, RED CRUSTED AREA, 6 MM IN DIAMETER (CAP 22) MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE -------------------------------------------- 3998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2237 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93219 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 761.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:38 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 3999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2238 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93220 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ *** ORGAN WEIGHTS WERE NOT RECORDED *** ------------------------------------------------------------------------------------------------------------------------------------ OBSERVATIONS LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ >APPEARANCE; MALOCCLUSION; VERIFIED AT LIVER (LI) : LIVER (LI) : NECROPSY: OBSERVED -RED FOCUS(I)/AREA(S); ALL LOBES: -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; MULTIPLE DARK RED AREAS, UP TO 4 MM IN -VACUOLATION, HEPATOCELLULAR, 70 X 60MM; VERIFIED AT NECROPSY: DIAMETER; COLLECTED ON ROUTINE PERIPORTAL,-SLIGHT OBSERVED SECTIONS -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,- MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT -INFILTRATION, FAT, INTERSTITIAL,- SLIGHT ADRENAL, CORTEX (AC) : ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT -PIGMENT,-SLIGHT IS 3 MM IN DIAMETER) -DEGENERATION / NECROSIS, UNILATERAL,- MODERATELY SEVERE -M-CARCINOMA,-NEOPLASM PRESENT 4000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2239 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93220 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ LN, MESENTERIC (MS) : LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN -INFILTRATE, MACROPHAGE, PIGMENTED,- SLIGHT LUNG (LU) : LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: -N-CARCINOMA,-NEOPLASM PRESENT MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM PITUITARY (PI) : PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN -HYPERPLASIA,-MODERATE -DARK FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE BROWN AREAS, UP TO 2 MM IN DIAMETER -LIGHT FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE WHITE FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION -CYST / GALACTOCELE,-PRESENT EXTENDING RIGHT PERINEAL REGION: -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT SINGLE MASS, 105 X 70 X 18 MM, RED, -HYPERPLASIA, GLANDULAR,-SLIGHT DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION 4001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2240 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93220 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ O B S E R V A T I O N S (CONTINUED) LAST CLINICAL OBSERVATIONS NECROPSY HISTOPATHOLOGY ------------------------------------------ ------------------------------------------ ------------------------------------------ MAMMARY, FEMALE (MF) : -MASS #2; LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 23 X 15 X 10 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) UTERUS (UT) : UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; -HYPERPLASIA, LYMPHOPLASMACYTIC,- COLLECTED MODERATE -HEMORRHAGE,-PRESENT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -------------------------------------------- 4002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 PAGE: 2241 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C93220 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE ------------------------------------------------------------------------------------------------------------------------------------ THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) ------------------------------------------------------------------------------------------------------------------------------------ 4003 APPENDIX 7 Cell Proliferation Report Covance 6329-183 3M T-6295 Note: This appendix contains information supplied by Pathology Associates International (PAI) and has been reviewed by the Quality Assurance Unit of PAI. 4004 Covance 6329-183 3M T-6295 4005 Covance 6329-183 3M T-6295 4006 Covance 6329-183 3M T-6295 4007 Covance 6329-183 3M T-6295 4008 Covance 6329-183 3M T-6295 4009 Covance 6329-183 3M T-6295 4010 Covance 6329-183 3M T-6295 4011 Covance 6329-183 3M T-6295 4012 Covance 6329-183 3M T-6295 4013 Covance 6329-183 3M T-6295 4014 Covance 6329-183 3M T-6295 4015 Covance 6329-183 3M T-6295 4016 Covance 6329-183 3M T-6295 4017 Covance 6329-183 3M T-6295 4018 Covance 6329-183 3M T-6295 4019 Covance 6329-183 3M T-6295 4020 Covance 6329-183 3M T-6295 4021 Covance 6329-183 3M T-6295 4022 Covance 6329-183 3M T-6295 4023 Covance 6329-183 3M T-6295 4024 Covance 6329-183 3M T-6295 4025 Covance 6329-183 3M T-6295 4026 Covance 6329-183 3M T-6295 4027 Covance 6329-183 3M T-6295 4028 APPENDIX 8 Electron Microscopy Report Covance 6329-183 3M T-6295 Note: This appendix contains information supplied by the Pathology Associates International and has been reviewed by the Quality Assurance Unit of Pathology Associates International. 4029 Covance 6329-183 3M T-6295 4030 Covance 6329-183 3M T-6295 4031 Covance 6329-183 3M T-6295 4032 Covance 6329-183 3M T-6295 4033 Covance 6329-183 3M T-6295 4034 Covance 6329-183 3M T-6295 4035 Covance 6329-183 3M T-6295 4036 Covance 6329-183 3M T-6295 4037 Covance 6329-183 3M T-6295 4038 Covance 6329-183 3M T-6295 4039 Covance 6329-183 3M T-6295 4040 Covance 6329-183 3M T-6295 4041 Covance 6329-183 3M T-6295 4042 Covance 6329-183 3M T-6295 4043 Covance 6329-183 3M T-6295 4044 Covance 6329-183 3M T-6295 4045 Covance 6329-183 3M T-6295 4046 Covance 6329-183 3M T-6295 4047 Covance 6329-183 3M T-6295 4048 Covance 6329-183 3M T-6295 4049 Covance 6329-183 3M T-6295 4050 Covance 6329-183 3M T-6295 4051 Covance 6329-183 3M T-6295 4052 Covance 6329-183 3M T-6295 4053 Covance 6329-183 3M T-6295 4054 Covance 6329-183 3M T-6295 4055 Covance 6329-183 3M T-6295 4056 Covance 6329-183 3M T-6295 4057 Covance 6329-183 3M T-6295 4058 Covance 6329-183 3M T-6295 4059 Covance 6329-183 3M T-6295 4060 Covance 6329-183 3M T-6295 4061 Covance 6329-183 3M T-6295 4062 Covance 6329-183 3M T-6295 4063 Covance 6329-183 3M T-6295 4064 Covance 6329-183 3M T-6295 4065 Covance 6329-183 3M T-6295 4066 Covance 6329-183 3M T-6295 4067 Covance 6329-183 3M T-6295 4068