Document xjkNjaRZ7mDnMw4D5GYE3gka6
A R HHG - O &KQ
ACUTE TO XICITY TO FISH
TEST SUBSTANCE ______________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-185F. (1Octanesulfonic acid) (CAS # 2795-39-3)
Remarks: The 3M production lot number was 1-2303. The test sample is
FC-185F, cc 817-19. Current information indicates it is a mixture of 4% PFOS, 36% diethylene glycol butyl ether, 29.29% water, 14% ethylene glycol,
8% Sultone foamer, 8.35% sodium octyl sulfate, 0.3% sodium lauryl sulfate,
and 0.06% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:_________________________________________________________
Method: ASTM -E729-80
Type: Static acute
GLP: No
Year completed; 1982
Species: Pimephales promelas
Supplier: Dale Fattig Fish Farm, Brady, NB
Analytical monitoring: Temperature, pH, and DO
Exposure period: 96-hours
Statistical methods: LC50values calculated using both moving average
angle and probit programs in conjunction with the 3M TRAC Computer
system
Test fish age: Juvenile
Length and weight:
Average length = 38 mm
Average weight = 0.38 g (wet)
Loading: 0.48g fish /L
Pretreatment: None
Test conditions:
Dilution water: Carbon-filtered well water
Dilution water chemistry:
Hardness: 252 mg/L as CaC03
Alkalinity:
225 mg/L as CaC03
Lighting: A daily photoperiod of 16 hours light and 8 hours dark with a 30
minute transition period was maintained throughout the testing period.
Stock and test solution preparation: Test solutions were created by direct individual weight additions. Concentrations dosing rate: Once
000119
S tability o f the test chem ical solutions: Not noted Exposure vessels: All-glass aquaria, 35x20x25 cm containing 16 liters of test solution Num ber o f replicates: 2 Num ber o f fish per replicate: 20 Num ber o f concentrations: five plus a blank control W ater chem istry during the study:
pH range (0-96 hours):
8.0 - 8.2 (control exposure)
7.7 - 8.2 (370 mg/L exposure)* Tem perature range (0-96 hours):
1 7 - 2 1 C Dissolved oxygen range (0-96 hours):
5.8 - 9.2 mg/L (control exposure) 1.3 - 9.3 mg/L (370 mg/L exposure)* * 370 mg/L (second highest concentration) exposure values used because the highest concentration used resulted in total mortality by 24 hours.
Rem arks: The dissolved oxygen concentration was below 60% saturation in all exposure concentrations except the control by 48 hours. This may have contributed to the observed mortality.
RESULTS___________________________________________________
Nom inal concentrations: Bk control, 100,155, 240, 370, and 560 mg/L Elem ent values: 96-hour LC50 - 344 (318 - 376) mg/L
Element values based on nominal concentrations
Rem arks: Testing was conducted on a mixture of as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical components alone.
C O N C L U S IO N S ___________________________________________________
The test sample 96-hour LC50for fathead minnow was determined to be 344
mg/L with a 95% confidence interval of 318 to 376 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking = 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
00010
REFERENCES Test was conducted by the 3M Company, Environmental Laboratory, Lab Request number 7544,1982. O T H E R _________________________________________________________ Last changed: 6/26/00
0001
TECHNICAL REPORT SUMMARY
Data 2/1/82
TO: TECHNICAL COMMUNICATIONS CENTER - 201-2CN
(Im p o rta n t -- I f re p o rt is p rin ted on both sides o f p aper, send tw o copies to TC C .) See " Guidelines fo r C om pletion o f Technical R ep o rt
Sum m ary" available from In fo rm atio n Liaison o r TCC (3 -5 5 4 5 ).
Division
Dipt.Number
Environmental Laboratory (EE & PC) Project
0535 ProjectNumber
Commercial Chemicals ReportTitle
9972210100 ReportNumMr
Aauatic Toxicitv Testing: FC-185F (cc817-19) L 1-2303 LR 7544 T
007
E. A. Reiner ter - 2-3E
Notebook Rafaranca
Employa* Numbtrltl
09362 No.ofPaga*IncludingCovantiaat
SECU RITY
KEYWORDS: Include Lab Code
Qi Ooan
ciosad
3M CH EM ICAL ^ R EG ISTR Y ^
Naw Chemicals Reportad
Ye
E No
* ^ Rc^ti^nune' the acute aquatic LC50, following exposure to varying concentrations of FC-185F (cc817-19) Lot 1-2303.
EE & PC-Div. (Env. Lab)
Toxicity (Aquatic)
______ ____________________________________ _________ ______________
REPORT ABSTRACT: This abstract inform ation is distributed by the Technical Communications Center to alert 3M*ers Company R&D.
The acute static aquatic 96-hour LCsn values obtained for fathead minnows (Pimcphales promelas) following exposure tc FC-185F in replicated experiments were 339 (not defined) and 372 (333-415) mg/1. The combined 96-hour LC 50 was 354 (0 ) (not defined) mg/1 as calculated using the probit analysis, statistic.
Data generated include LC50 values with 95% confidence
limits
;
Inform ation Initials:
000122
FC-18 5F/ANW Page 2 February 1, 1982
CONCLUSIONS
In these replicated 96-hour LC50 aquatic tests, the calculated values for fathead minnows (Pimephales p r o m e l a s ) were 339 and 372 mg/1. Materials possessing an L C 50 o this order of magnitude are considered to be practically nontoxic based on the NIOSH criteria for aquatic toxicity interpretation. (2 )
INTRODUCTION
The test substance is a foamy, clear, dark amber liquid, soluble in water at ambient room temperature. Tests performed on this material evaluated its potential aquatic toxicity and the data generated form the basis of this report.
ACCLIMATION AND EXPERIMENTAL DESIGN
The test protocol utilized for this study was modeled after that described by ASTM-E729-80. (1)
The fathead minnows (Pimephales p r o m e l a s ) were obtained from a private hatchery (a). Stock fish were held in a flow through system fiberglass holding tank filled with carbon-filtered well-water at 14-16 degrees C. A daily photoperiod of 16 hours light and 8 hours dark, with a 30-minute transition period was maintained throughout the acclimation and testing period. The fish were fed Tetra-Min (b) daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated to test temperature for 2 days prior to use in assays.
Twenty (20) fathead minnows, uniform in size, were tested at each concentration of chemical. All-qlass aquaria, 35x20x25 cm containing 16 liters of diluent plus toxicant comprised a study chamber. Test fish were randomly allocated to each test chamber within 30 minutes following toxicant addition. The fathead minnow experiments were conducted at 19+2 degrees C. Mortality, temperature, dissolved oxygen level, and pH oF all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations were recorded relative to swimming pattern and behavioral changes induced by exposure to the test chemical.
The acute short-term static bioassays were performed on FC-185F using carbon-filtered well water of known composition and constant quality as the diluent (Tables 1,2).
a) Dale Fattig Fish Farm, Brady, Nebraska. b) Commercial Fish Food of known composition.
000123
FC-18 5F/ANW Page 3 February 1, 1982
The 96-hour fish studies were performed in replicate using the following FC-185F logarithmic concentrations: 100, 155, 240, 370, and 560 m g / 1 . This range of dilutions was selected to bracket 96-hr LC50 values predicted from preliminary exploratory toxicity tests.
LC 50 values with 95% confidence limits were calculated usinq both moving average angle and probit programs in conjunction with the 3M TRAC Computer system.
Monitoring equipment used included ASTM thermometers, Orion temperature-- compensating pH meter, a Yellow Springs dissolved oxygen meter, and a Taylor temperature chart recorder.
RESULTS
The fathead minnows (Pimephales promelas) used in these studies had an average weight of 0.4 grams and a length of 3.8 cm. The loadinq factor was 0.48 g/1; the maximum recommended loading factor is 0.8 g/1 (1). Temperature, pH, and dissolved oxygen values and ranges obtained during this study were: 19+ 2C, 7.7-8.2 pH units, and 96-hour dissolved oxygen values ranged from 1.3-5.9 mg/1. These latter values calculated out to a minimal oxyqen saturation of 14-63%.
In this replicated study, the fish 96-hour LC50 values were 339 and 372 mq/1. Chemical possessing an LC50 value in this range are considered to be slightly toxic to the aquatic environment (2 ).
The data reported herein are based on studies performed by H. T. Elnabarawy and R. R. Robideau. Accompanying data sheets comprise original data. A copy of this report is filed in the Environmental Laboratory Archive System, Building 2-3E.
It should be noted that the generated results were reported in the absence of information relative to the "purity" and the "stability" of the chemical substance. The reported LC50 values were calculated on the basis of the "observed mortality rates" and of the calculated initial concentrations of FC-185F in test solutions.
REFERENCES
(1) ASTM-Standard Practice E729-80. Standard Practice for Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates, and Amphibians. American Society for Testing and Materials, Philadelphia, PA 1980.
(2) Registry of Toxic Effects of Chemical Substances, '76 Edition, Christensen, H. E., and E. J. Fairchild, Eds., p. XV and Ci, U.S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, D.C. (June, 1976).
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TABLE 1
WELL WATER QUALITY
SAMPLE DATE: 1/28/82 SAMPLE TAKEN: 3M Bldg. 2-3E, St. Paul
SAMPLE DATE 1/28/82 (TAKEN PROM ROOM 5)
PARAMETER
CONCENTRATION
ENV. LAB WATER QUALITY BOOK REP.
pH Residual Chlorine Ammonia Nitrogen Conductivity M-Alkalinity Total Solids Total Hardness Total Fluoride Dilute COD Total Coliform Standard Plate Count
7.7 <0.02 mg/1 <0.1 mg/1 as N 330 umhos/cm1 at 25C 223 mg/1 as CaC03~ 315 mg/1 252 mg/1 as CaC03 <0.02 mg/1 <0.4 mg/1 Negative 21 per ml
8 9 8 9 8 8 8 9 9 10 10
Aluminum as Al* Arsenic as As* Boron as B* Cobalt as Co* Copper as Cu* Chromium as Cr* Cadmium as Cd* Iron as Fe* Nickel as Ni* Lead as Pb* Silver as Ag* Zinc as Zn* Mercury as Hg*
0.2 mg/1 <0.005 mg/1 <0.2 mg/1 <0.02 mg/1 <0.02 mg/1 <0.03 mg/1 <0.01 mg/1 <0.02 mg/1 <0.02 mg/1 <0.05 mg/1 <0.02 mg/1 0.05 mg/1 <0.2 ug/1
14 14 14 14 14 14 14 14 14 14 14 14 14
PCB's**
ND
95
Total Organochlorine** Pesticides
ND
95
Total Organophosphorus* *
Pesticides
ND
95
* Sample Date 1/28/81 **Sample Date 3/4/81
0001
TABLE 2
ENVIRONMENTAL LABORATORY BLDG. 2-3E
ST. PAUL, MINNESOTA
FOURTH QUARTERLY REPORT, 1981 WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING
PARAMETER
NOV 2
TOTAL HARDNESS* as mg/1 CaCO
248
TOTAL "M" ALKALINITY* as mg/1 CaC03
228
ELECTRICAL SPECIFIC CONDUCTANCE* as umhos per cm2 at 25C
330
TOTAL SOLIDS* as mg/1
320
pH**
7.6
NOV 30 252
DEC 29 256
AVERAGE (+ S)
252 4
226 221 225 4
340 380 350 + 27
1+ i->
300 308 309 7.6 7.7 7.6 + 0.1
STANDARD PLATE COUNT*** per 1.0 ml
2
10-
27 13 13
CRITERIA OF CONSTANT QUALITY
* Monthly ranges should be less than 10% of their respective averages. (ASTM Standard E729-1980.)
** Monthly range of pH should be less than 0.4 unit. (ASTM Standard E729-1980.)
*** Total bacterial densities should not be greater than 500 organisms per ml. (USEPA-600/8-78-017, p. 101.)
000126
ENVIRONMENTAL LABORATORY
AQUATIC TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST)
LR
pe & H C T E . S r f t r ^ t e r i a l Tested F C - IftSF T ft11 - IS lo t j
st Organism FftWEAft MIKIMODSScurce-^^rtlTr, U e R Date Rac'd || /SO/fll Batch > )-j
Cg-wt-CLSSgrams Avg. Size-5l8 cm No. Organisms per dose l Loading Factor
.sh Disease Treatment: M o N f - _____________________________________________________________
ist Solution VolumeJJa. Liters/All-Glass Tank Tank Dimensions: 35x20 cm and 25 cm deep
me Started \'
ZT M _Date Started \fit*jets _ __Date Ccmpleted i / a > / 8 j
.lution Water Carbon-filtered well water (Temp. )/ C., D.O. ^ ^ ppm, pH_ 8 . Z j
)
ivostigator
_________ LC^q
Confidence Limits)
M il Signature) iW fc lT ANALYSIS 3 3 ^
_____ MOv/tM^ AVERAGE. AKMjCE. M E T H O D
(U o U H Cts) C e M f c i (U <LU M S&
(2au>-3g&)**f* ( CoH&vfeb &VU3S>\&-&Uo)VK,
Pank Initial Total
24 Hours
48 Hours
to. Cone. Amount Total No. pH D.O. Total No.
mg/1 Added
Dead
PFTM Dead
72 Hofg"
96 Hours
Total No. pH D.O. Total No. pH D.O
Dead
ppn Dead
Ppn
*0
GJL Contro)
'3L Ccvng
ZA1 AlCl lla . 25S. a t a o
lalao L l 2tS
n ____ 1 2
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5. 2Q. ^ 4 0 1 2 ____1 2 % 2
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Test Temp.
ki^um.
tii
icondary Effects (other than Mortality):
-m a r i a x --g&rnM. -TTMtiespnxKfs
EC50_________________________
ENVIRONMENTAL LABORATORY
AQUATIC TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST)
lr_ # 5 3 ^
P0 Tes-q bttR . A CUTE
Material Tested F O l f t S F ftL
-st Organism ^ n t t B ^ M l M K k ^ Source
M g f r Date Rec'd \\J&gfo\ Batch # l 8 L)
/g.Wt. 0, ^figrams Avg. Size * * ^ ^ cm to. Organisms per dose 2 Q^ Loadi_n_g___F__a_c_t_o__r Q- v, .1
g/
Lsh Disease Treatment: _ M o m
3st Solution Volume -Ua-Liters/All-Glass Tank Tank Dimensions: 35x20 cm and 25 cm deep
scondary Effects (other than Mortality) :
Tank No.
Symptoms or Reactions
ontrol\
24-Hr.
JMIR0L \L
1T> /`StjjpP'rtriLiA 23> ^SilWaAfiUA
W d m ^.
WoU
Ko w e
Tk nue cue.
EC50
Total Number Affected
48-Hr.
72-Hr.
96-Hr.
Sfoue. S ____ _
0
5
AJMfi
kifijG
\s
20
20
12j _
Afonie. A/rw Za 2r> te?
IZJ
-- :--- 0 Q C 1 3 S --
ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST)
TYPE TEST^frrtK,
MATERIAL T E S T E D L f > T
TEST ORGANISM,
MINIMAUX
\-23Q3
LC5 0 's (95% CONFIDENCE LIMITS) PROBIT ANALYSIS
ST RUNS
UN CL
74 HOURS
48 HOURS
353*^2
72 HOURS 3 5 3 f(
( W o u m o s .) (.Mo U M I T S )
96 HOURS 334*^2
(Mo limits)
COMBINED 96 HOURS
UN O
4 n S ti (423-523
"S^ZjymfX (335-HlS^
M o ljmxts}
MOVING AVERAGE ANGLE METHOD
:3T RUNS
.u n CL
UN D
24 HOURS
48 HOURS
72 HOURS
96 HOURS
370
sro^ff 3 3 4 ^
(5n-45lw$) fezn-4s\^) (RtrS^Mj^
443 (4s-46(t^l)
383-^2
373
555
(3Me-sfn*$) (52i-3f8^i)
COMBINED 96 HOURS
344 ( 2 > l 8 ~ S "7l
0001^9
ENVIRONMENTAL LABORATORY AOUATIC TO XICITY DATA SHEET EXPLORATORY (R A N G E -F! NO I NG TEST)
I S44
TVPe Test ^ b U . f e u l P S r ftT U L ^ M a te r ia l Tested
T e st O rg a n isB B V TM B to M tV lU fll Sou rce
K te fi.
Da te Rcc' d .u / g p / f K Batch N o.^-
2Avg. W t. f ) X i ______ grans Avg. S i eS , l | CM No. O rganisas p e r dose
5______ ^
T e s t S o lu tio n Volume 3 Lit e r s / A ll- G la s s Tank Tank Dimensions:WIDE-MOUTH 1 GALLON GLASS
11Time Started^ ;
1Date S ta rte d i . / i q / f f o _______ Date Completed^ j i ^ / m ,
D ilu t io n Water C a r b o n - filte r e d w e ll w a te r (Temp. ZJ C. D.O.
ppm, pH /
In v e s tig a to r'~ ~> ^//i^f^ 1,
LC5Q (9S% C o n fia n c e L im its )
inrM.i. pat, inpf//r>/pa i t - ^ - 3 /
Coanents: * I u m n in in U .' W n ^ t fTTv. ~tfe*cr
JM g* * m h=^ uifynrv <VLUW-C. m
) IS A
Cl Q M ^ -
^A M B aair-tyadTEiH B ^
E ffe c ts o th e r than M o rta lity
E^50-
Tank No.
C ontrol IA
2A ."K A
Symptoms o r R eactions
* k l s Prifrsen. J T
5. i A _
(.'omments :
2 4 -H r.
h / A /L A /A A /aa 4 /a jjf
_
T o ta l No. A ffe cte d
48 H r.
72-Hr.
M me A/a/. A /sa/C .
/U ja/ A ftU . i u / G ...
r\P
9-H r.
-
/
V
0001C0
